LOCUS       DAZUKD010000001        68861 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000001 DAZUKD010000000
VERSION     DAZUKD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.001"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1432)
                     /gene="pepV"
                     /locus_tag="ACG8N9_000001"
     CDS             complement(14..1432)
                     /gene="pepV"
                     /locus_tag="ACG8N9_000001"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4895645.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            1581..2426
                     /locus_tag="ACG8N9_000002"
     CDS             1581..2426
                     /locus_tag="ACG8N9_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4895646.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2430..2870
                     /locus_tag="ACG8N9_000003"
     CDS             2430..2870
                     /locus_tag="ACG8N9_000003"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4895647.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            2920..3114
                     /locus_tag="ACG8N9_000004"
     CDS             2920..3114
                     /locus_tag="ACG8N9_000004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895648.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            3250..3935
                     /locus_tag="ACG8N9_000005"
                     /pseudo
     CDS             3250..3935
                     /locus_tag="ACG8N9_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(4343..5032)
                     /locus_tag="ACG8N9_000006"
     CDS             complement(4343..5032)
                     /locus_tag="ACG8N9_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895649.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(5007..7143)
                     /locus_tag="ACG8N9_000007"
                     /pseudo
     CDS             complement(5007..7143)
                     /locus_tag="ACG8N9_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
     gene            complement(7191..8528)
                     /locus_tag="ACG8N9_000008"
     CDS             complement(7191..8528)
                     /locus_tag="ACG8N9_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4895650.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(8780..9139)
                     /gene="rplT"
                     /locus_tag="ACG8N9_000009"
     CDS             complement(8780..9139)
                     /gene="rplT"
                     /locus_tag="ACG8N9_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4895651.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(9192..9392)
                     /gene="rpmI"
                     /locus_tag="ACG8N9_000010"
     CDS             complement(9192..9392)
                     /gene="rpmI"
                     /locus_tag="ACG8N9_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4895652.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(9464..9988)
                     /gene="infC"
                     /locus_tag="ACG8N9_000011"
     CDS             complement(9464..9988)
                     /gene="infC"
                     /locus_tag="ACG8N9_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4895653.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(10032..10157)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            10310..10942
                     /locus_tag="ACG8N9_000012"
     CDS             10310..10942
                     /locus_tag="ACG8N9_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4895654.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(10944..11849)
                     /locus_tag="ACG8N9_000013"
     CDS             complement(10944..11849)
                     /locus_tag="ACG8N9_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4895655.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(11934..12593)
                     /locus_tag="ACG8N9_000014"
     CDS             complement(11934..12593)
                     /locus_tag="ACG8N9_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4895656.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            12909..13853
                     /gene="mvk"
                     /locus_tag="ACG8N9_000015"
     CDS             12909..13853
                     /gene="mvk"
                     /locus_tag="ACG8N9_000015"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4895657.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            13846..14823
                     /gene="mvaD"
                     /locus_tag="ACG8N9_000016"
     CDS             13846..14823
                     /gene="mvaD"
                     /locus_tag="ACG8N9_000016"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4895658.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            14836..15921
                     /locus_tag="ACG8N9_000017"
     CDS             14836..15921
                     /locus_tag="ACG8N9_000017"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4895659.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            15918..16982
                     /gene="fni"
                     /locus_tag="ACG8N9_000018"
     CDS             15918..16982
                     /gene="fni"
                     /locus_tag="ACG8N9_000018"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4895660.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(17231..18034)
                     /locus_tag="ACG8N9_000019"
     CDS             complement(17231..18034)
                     /locus_tag="ACG8N9_000019"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4895661.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(18054..18896)
                     /gene="kduI"
                     /locus_tag="ACG8N9_000020"
     CDS             complement(18054..18896)
                     /gene="kduI"
                     /locus_tag="ACG8N9_000020"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4895662.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(18912..19919)
                     /locus_tag="ACG8N9_000021"
     CDS             complement(18912..19919)
                     /locus_tag="ACG8N9_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4895663.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(19926..20570)
                     /locus_tag="ACG8N9_000022"
     CDS             complement(19926..20570)
                     /locus_tag="ACG8N9_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4895664.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(20670..21428)
                     /locus_tag="ACG8N9_000023"
     CDS             complement(20670..21428)
                     /locus_tag="ACG8N9_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4895665.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(21555..22706)
                     /gene="fucO"
                     /locus_tag="ACG8N9_000024"
     CDS             complement(21555..22706)
                     /gene="fucO"
                     /locus_tag="ACG8N9_000024"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4895666.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(22788..23099)
                     /gene="rhaM"
                     /locus_tag="ACG8N9_000025"
     CDS             complement(22788..23099)
                     /gene="rhaM"
                     /locus_tag="ACG8N9_000025"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4895667.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(23101..23946)
                     /gene="rhaD"
                     /locus_tag="ACG8N9_000026"
     CDS             complement(23101..23946)
                     /gene="rhaD"
                     /locus_tag="ACG8N9_000026"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4895668.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(23960..25216)
                     /gene="rhaA"
                     /locus_tag="ACG8N9_000027"
     CDS             complement(23960..25216)
                     /gene="rhaA"
                     /locus_tag="ACG8N9_000027"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4895669.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(25213..26673)
                     /gene="rhaB"
                     /locus_tag="ACG8N9_000028"
     CDS             complement(25213..26673)
                     /gene="rhaB"
                     /locus_tag="ACG8N9_000028"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4895670.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            26826..27695
                     /locus_tag="ACG8N9_000029"
     CDS             26826..27695
                     /locus_tag="ACG8N9_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4895671.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(27821..28720)
                     /locus_tag="ACG8N9_000030"
     CDS             complement(27821..28720)
                     /locus_tag="ACG8N9_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4895672.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(28885..29709)
                     /locus_tag="ACG8N9_000031"
     CDS             complement(28885..29709)
                     /locus_tag="ACG8N9_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4895673.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(29720..31015)
                     /locus_tag="ACG8N9_000032"
     CDS             complement(29720..31015)
                     /locus_tag="ACG8N9_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4895674.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(31031..31510)
                     /locus_tag="ACG8N9_000033"
     CDS             complement(31031..31510)
                     /locus_tag="ACG8N9_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4895675.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(31516..32544)
                     /locus_tag="ACG8N9_000034"
     CDS             complement(31516..32544)
                     /locus_tag="ACG8N9_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4895676.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(32561..33667)
                     /locus_tag="ACG8N9_000035"
     CDS             complement(32561..33667)
                     /locus_tag="ACG8N9_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4895677.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(33896..36493)
                     /gene="adhE"
                     /locus_tag="ACG8N9_000036"
                     /gene_synonym="adhC"
     CDS             complement(33896..36493)
                     /gene="adhE"
                     /locus_tag="ACG8N9_000036"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4895678.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(36798..37178)
                     /gene="yajC"
                     /locus_tag="ACG8N9_000037"
     CDS             complement(36798..37178)
                     /gene="yajC"
                     /locus_tag="ACG8N9_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4895679.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(37275..38420)
                     /gene="tgt"
                     /locus_tag="ACG8N9_000038"
     CDS             complement(37275..38420)
                     /gene="tgt"
                     /locus_tag="ACG8N9_000038"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4895680.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            38638..39336
                     /locus_tag="ACG8N9_000039"
     CDS             38638..39336
                     /locus_tag="ACG8N9_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4895681.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(39504..42143)
                     /locus_tag="ACG8N9_000040"
     CDS             complement(39504..42143)
                     /locus_tag="ACG8N9_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4895682.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(42762..43793)
                     /gene="queA"
                     /locus_tag="ACG8N9_000041"
     CDS             complement(42762..43793)
                     /gene="queA"
                     /locus_tag="ACG8N9_000041"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4895683.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            43986..45161
                     /locus_tag="ACG8N9_000042"
     CDS             43986..45161
                     /locus_tag="ACG8N9_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4895684.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            45165..45806
                     /locus_tag="ACG8N9_000043"
     CDS             45165..45806
                     /locus_tag="ACG8N9_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4895685.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            45803..46720
                     /locus_tag="ACG8N9_000044"
     CDS             45803..46720
                     /locus_tag="ACG8N9_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4895686.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            46717..47388
                     /locus_tag="ACG8N9_000045"
     CDS             46717..47388
                     /locus_tag="ACG8N9_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4895687.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(47929..48417)
                     /locus_tag="ACG8N9_000046"
     CDS             complement(47929..48417)
                     /locus_tag="ACG8N9_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4895688.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(48618..48884)
                     /locus_tag="ACG8N9_000047"
     CDS             complement(48618..48884)
                     /locus_tag="ACG8N9_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4895689.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(48884..49147)
                     /locus_tag="ACG8N9_000048"
     CDS             complement(48884..49147)
                     /locus_tag="ACG8N9_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4895690.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            49668..49808
                     /locus_tag="ACG8N9_000049"
                     /pseudo
     CDS             49668..49808
                     /locus_tag="ACG8N9_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(49950..51773)
                     /locus_tag="ACG8N9_000050"
     CDS             complement(49950..51773)
                     /locus_tag="ACG8N9_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4895691.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(52371..55076)
                     /locus_tag="ACG8N9_000051"
     CDS             complement(52371..55076)
                     /locus_tag="ACG8N9_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4895692.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(55382..56329)
                     /locus_tag="ACG8N9_000052"
     CDS             complement(55382..56329)
                     /locus_tag="ACG8N9_000052"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4895693.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            56519..56788
                     /gene="rpsN"
                     /locus_tag="ACG8N9_000053"
     CDS             56519..56788
                     /gene="rpsN"
                     /locus_tag="ACG8N9_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4895694.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(56964..57326)
                     /locus_tag="ACG8N9_000054"
     CDS             complement(56964..57326)
                     /locus_tag="ACG8N9_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4895695.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(57337..58458)
                     /gene="alr"
                     /locus_tag="ACG8N9_000055"
     CDS             complement(57337..58458)
                     /gene="alr"
                     /locus_tag="ACG8N9_000055"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4895696.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(58474..58824)
                     /gene="acpS"
                     /locus_tag="ACG8N9_000056"
     CDS             complement(58474..58824)
                     /gene="acpS"
                     /locus_tag="ACG8N9_000056"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4895697.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(58954..60465)
                     /locus_tag="ACG8N9_000057"
     CDS             complement(58954..60465)
                     /locus_tag="ACG8N9_000057"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4895698.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(60787..62139)
                     /gene="murF"
                     /locus_tag="ACG8N9_000058"
     CDS             complement(60787..62139)
                     /gene="murF"
                     /locus_tag="ACG8N9_000058"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4895699.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(62292..63368)
                     /locus_tag="ACG8N9_000059"
     CDS             complement(62292..63368)
                     /locus_tag="ACG8N9_000059"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4895700.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            63564..65309
                     /locus_tag="ACG8N9_000060"
     CDS             63564..65309
                     /locus_tag="ACG8N9_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4895701.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(65589..66026)
                     /locus_tag="ACG8N9_000061"
     CDS             complement(65589..66026)
                     /locus_tag="ACG8N9_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4895702.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(66010..67383)
                     /locus_tag="ACG8N9_000062"
     CDS             complement(66010..67383)
                     /locus_tag="ACG8N9_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895703.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(67705..68193)
                     /locus_tag="ACG8N9_000063"
     CDS             complement(67705..68193)
                     /locus_tag="ACG8N9_000063"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4895704.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(68215..68817)
                     /locus_tag="ACG8N9_000064"
     CDS             complement(68215..68817)
                     /locus_tag="ACG8N9_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4895705.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        19652 a        14868 c        12608 g        21733 t
ORIGIN      
        1 ttttttagta tttttatttc gccaaacgat agatggcatc tgcatagatt gctgttgctc
       61 tgaacaagtc atcaagactc ataaattcgt ttgcttgatg cattgtatca gtatatcctg
      121 ggaacatcgc tccataagca acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga
      181 tgatttgttc gtagcctttt tctccagtgt ggtcttcata gacttgcaat aatgtagcca
      241 ccaatggatc atccattggt acgtaatgtg gttccattac gcgagcacct cgagtaactg
      301 ttgcgccttc ttgccccact gtttgttcga tgtcactttg caatccgtct actgttacac
      361 cttttggaaa acggaaattc atattgatga agttcgcttc ctctgtacca ttttcttcaa
      421 aagcaaatag tccagcattc attgtcaatt tgcccatttt ttcatcttca taagcgactc
      481 ctagtttttc tccataaaaa tcttcatgga cataagcagc tgctacatgg ataaattgtt
      541 tagcagatcc taggaattca tagttatcta agaacaatgc caagaaagaa cctgcattga
      601 taccagattg tgggcttgca ccatgtgcgc ctttaccaac taattcgatt tttacatacg
      661 tattgtccgc ttcaatcgtt ccgcttactg gatttgcttc gatgaattgg taaaatgctt
      721 cttccatcgc gatagctgca tctgcacttg gtacttgaag tgctgcagtt gctgttcctg
      781 gcaccatatt ttcgcgaagt ccggaaacaa aagatttcaa gacataatct cctgcattgt
      841 cgcctttgaa gcgtaaagct aaagaaacat ttcctttttc accattgatg attgggaatt
      901 cagcatctgg tgagaaacca aaatctggtt cttcttcatg ttcgaaataa taagccatat
      961 cgccccagcc gctttcttca tcgctgccga ctacgaagcg aacttttttt gatagtttca
     1021 agtcaagttc tttaatgatc ttcatcgcat agtaagctgc catacttgga cctttgtcat
     1081 cgcttgatcc gcgtgcaaag atctttccat ccttgattac tggttcataa ggatctgtgt
     1141 cccagccatc tcctgcagga acgacatcca tgtgtccaaa tatccctagc gtttcgtctc
     1201 cttcgccaag atcaatatgc cctgcatagt tatcgacatt ttttacttta aatccatcac
     1261 gttcgccata agctaacata tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat
     1321 ctggtgttac ttttgaatca tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt
     1381 caagcaagtc ttctttacgt gcttctactt ctttttgcca atcaattgtc ataaagttaa
     1441 tcctccactt atttaagtct tcgtgttcat gataccacaa attacagaaa aaaatagttc
     1501 aaaaaatctg acaattcctt ttcttatctg tttttctcta aaaaaaatat acgctataat
     1561 gagtaaaagg aggtttttcg atgctgataa acgaacaact agtgaaaaga gtcatcacac
     1621 ctcgtccaaa gatttcacat aaaggaacat ttggacgtat cgttttgatt ggcggcaatg
     1681 aacaatatgg cggagcaatc attatgagtg ctttagctgc cgtacacagt ggagcgggtc
     1741 tcacgactgt agtaactgat gaaaaaaatc ggagtgctct tcacagtcat ctgccagaag
     1801 caatgattgt agattggacg gatactgacc agtttctttc cgtaataaag caagcagatg
     1861 tactcttgat tggtcccgga ttgggtactt cttctaagag tttggaacgt ttcaaacaag
     1921 tactagaaaa ccaatcaaaa gaacaatggc tggtgattga tggctctgct ttaagtttac
     1981 ttgttgctaa tcatcttttg ctaccctatc ccgaacaaac tgtacttaca cctcatcaaa
     2041 tggagtggca acggttgagt tctctaccga tacaagaaca acgggataaa gaaaacaaaa
     2101 agaagcaaca ggaaatcggt gctgtgctcg ttttgaaaag tcatgagaca aaaatttatg
     2161 gtcaccagca cttctacacc aatcctttag gttctgctgc tatggcaaca ggcggcatgg
     2221 gtgacacttt agctgggatg atcacaggat ttttagccca gtttcctcaa aatgaccaaa
     2281 cgattgcagc agctgtttat cttcatagtt tcattggaga ccagctggca aaagaacaat
     2341 atattgtttt gccgacacaa atcagcgaag cacttccgaa atggatgaat tatttttgta
     2401 aaaaatcaat ggaaaacgag gcatgagtca tggagaaatt tacaggttat gtgcgagaac
     2461 ctttttatta tgaaacagat caaatgggga ttattcatca cagcaattat atccgctggt
     2521 ttgaagaggc tcgggtagcc ttattggtac atctagggtt tccctataaa aaaatcgaag
     2581 aatctgggat catcatcccc gttctggaag taaattgcag ctataaagaa atgatccatt
     2641 acggagaaaa agtgagcatc catccaacta tccaaaagta tacggggaca cggttggatt
     2701 ttagctacga aattgtggga actgaagaca aaaaattaaa aactactggc agtagtaaac
     2761 actgtttttt actttctgat tcgcaacgat tagtgaagct gagcaaagtc aatccggagt
     2821 tagatcagtt attccaagcg tacgcttctt tggatgatgt aaattcttaa taaagaccgt
     2881 ctaagcattt ttcttgtatt gtgtcgagta gttcgtataa tgaaaagaaa aaagcaggtg
     2941 atccccatgt atgtcgttaa agtaatgcac ggttacatcg ataaaactgg ctgtcgcact
     3001 cgggaaaaaa acttagacaa tctgctcatt ttcaaagaca aaaaagaatc cgaagctttt
     3061 gctaaaagaa tcggcggtcg tgtcaaaccg atccaagagg tacgaccaga ttgattcaat
     3121 atttttcttt tcgaggctct aataggcatg cctatcaggg ctttttttgc agactgtgtt
     3181 tttatgccca cacgatttgt aagcgattta tgttataata gattatatct gcatagaaag
     3241 gaaggttaaa tggttatcaa taatttgcaa cagcaagctt atgaatctat acgtaaacaa
     3301 atcatttatt ccgagttagc acctggaagt aaaatctccg ataaagtgtt agaagaacaa
     3361 ctagctatcg ggcgtacacc gattcgagaa gcactgatcc aactgcgcaa tcaagagctt
     3421 gtaaaaacca ttccacagtc cggcacttat gtttcaaaaa tcgatatccg atctgcttca
     3481 ttagcccgtt atacgcggga aaagttggaa aaaccgatcc tgcaagaatg cagtgcgaaa
     3541 atgaccaagc aagatcaagc taaactagaa aaaatactag aacaaaccga tcaggcagta
     3601 atcgctcaaa ataaaaaagc ttttttgatt tggatactga ttttcaccga acttgttatg
     3661 aaattgctgg aaaacgagaa atttgggatt ggctagaaag ttatagtacg cacttgaacc
     3721 gtttcagatg gttacgtttg acgatttcag aattagactg gggtcgagta ttagatgaac
     3781 atcagacaat gcttcaatca atgattgatc ataactttga tgaagtcgga tttctttgca
     3841 cgatgcattt acatatgatt atcgaggagc aagaatacgt gattcataat taccccgatt
     3901 attttgaaga tattcaggac agacccatca aataagaggt tttggaatca gcgttttgac
     3961 ctgagtatta gagaaagaaa cggcaaaata gttcctcata ttttgccgtt ttttttgagc
     4021 taataccgag ggtcagctga ttgcacaccg tttattgaga ggttgtgaaa aagctgccct
     4081 gcatcaagta ataagaacag ccaagtaaaa atagcttctt atgtttttaa cttggctgga
     4141 cttattaccg cagatgcaag cttttgaaca ccgttttcca agaggtcgtg agcctgccgt
     4201 tcatctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg ttgaatattt
     4261 tgacttgtga ccgaggagtt gacaggagaa catcgtttat tcggaaaaaa aggactgtga
     4321 cacgactttt gtcacagtct ctttatttgt aatctaaatc atactccgta catctttttt
     4381 ctattttttt tatttcttct aagatccctt ggctacgaat ttcattatat ggccaaagta
     4441 agactgcctc taatacataa ggcgaaaaat cgctttgatt catctctttt gaaaaactca
     4501 tcgctgctat ctcgctcaat gcacgaatgt ttgattgatg tgtataaaaa ggcaaccgcc
     4561 acagcacaat tttttcgctt tgcgtataaa ttcccttaac agaagcttcg atgtggtgaa
     4621 atagttcatg tccaataatc aaagcttgcg ctgtttcttt agagatcgaa gctagtcctg
     4681 aacattttaa tttttctaac ggctccgtca tcaatcgaat cccttcatct tctgcataag
     4741 ttgcaaaagt gattcgttcg tttatttgct ctgattcaca ggaaacgatt ggaacagcca
     4801 tttgttcaca aatttctcct acatcggcaa aagggtactt ttttttgatg ctacatgccg
     4861 tttcgatacc acaatcgatt gattgttgta taagaatttt ctgcttggac aaaggaatct
     4921 ttttccaaaa taaatctcgc cgaaactggt agaatcccca aatttcttcg gggatctgtt
     4981 gtaattcgtt tagtaactct tctaacttat gtttgtttga tatttccggc aacgacgatc
     5041 acttcttcct ccggttccat agtagatact tccagatcca tcaaaagctg tagttgttcc
     5101 agatccgtcg ctgtaaaatc agagactctt gctcctaagc agagataaaa ttctggtcgg
     5161 gcaatcaatt cagtttgata aactgccata tcttcccaaa gttgttcgac agctgtcata
     5221 taattggctg cagttgtttt catacatgaa gcatgtccac gttggacctt gataaatcct
     5281 ttttgatcga tgatccgaag acttccagca ttttttgaac gattctgttc gccgcataca
     5341 taaaagaaag gtgtattttc caataagcaa acttcatttt tatttaatcg catatcctct
     5401 gcagcaagct ctagtgcttc ttctgttgtg atctccttgg ttagatcggt tgctttcact
     5461 tccgtagaac cagtagcgat agccgttact tttgaagtct gtggatcgat ttctacgtgg
     5521 acttcgatcg attctggtgt agcgcctgat tcaatcgctt tattcatcgc ttcatttttc
     5581 aacgatcgta tatcttcttt acttggcgaa gggatgatac gttctacaac atctcggacc
     5641 attgcaagtg ctacaccaat agaagaaatc acttcggcgt tttcaggaat cgaatatttc
     5701 acacccattt tattactaaa gtaagtaatc aatgaggcag cgccgccccc tacacctaca
     5761 agagaaatct gatctttctc caagtgatac ttgtctgcca attctaaaat caccggttca
     5821 atcttagcat aggatttttc catgatctgt tctgcgatgt cttcaaccgt cgtatgacaa
     5881 taatcagcta atgcttggat ggcttttcgt gcagatggga catttccata agaaaaatgt
     5941 tcttcttgca ctaatccaag gacattggct gcacatgtat ttgtaatagt cacttcttct
     6001 ccgtcttcca tcactacctt gacgtaatca gcagggtctc cttctttagg agagaaaaat
     6061 tcaaccttag gacctttgat tttttctgtc tccgtaaata ctgcataatc tagaccggca
     6121 atatgtgcag aacgtggacc gacatcgatg attcctgact gattagcccg taccatcgaa
     6181 ccgcctgcca cacctagaac ccgaacatct aatgaagaga tatatgttgg gtggccggtg
     6241 gccacctaca attgaataat cgattgctgg acgtccattt ttaatgaccc cgatattggt
     6301 tgttgttccg cctacttcaa aataaacacc attagacgct ctcaagtaca tcaaagagcc
     6361 catgactgat gctgctggac cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt
     6421 gatttccatt actccgccgt ctccgcgcat aatcatcaaa gaaacattga cccctgcttc
     6481 tcgtacggaa tcttctgtag aggttgccgt atctagcatt tttggtaaaa tagatgcatt
     6541 gattgctgct gtccgagttc ttcttgtcaa tccatataat tttgtgatgt cagaagccat
     6601 cgttgtaggg atcgatttta ccgatgctgc ttcataaaca actcgttctg gttccccatt
     6661 atctactcca aatgccatag aagaaaccaa gacctccgct ctttctcttt ctaaagaaga
     6721 aatcgtttct gaaacacggt ctacatttaa gtgtttgaca ggtaagaatg cattgacaat
     6781 ctctattttt ttcttattcc ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa
     6841 aaaaccttct aatccacctt ttgccatccc gattacgccg acttttgcta cgtctccttc
     6901 aatcagtgca ttagtcgctt gtgtggtaga atgtgcaaca aacacaacat cttctggact
     6961 gatattattt tccagcaaac aattttgaaa agactgaacg acacctgcgg ctactccacg
     7021 gacatcatcg tgggttgttt tcactgaaga ttttccgatg atttcgtgcg tcgcattatc
     7081 gattgcgact gctttcgtat gtgtaccgcc aacatcaatt cccattcgca caaaacgttt
     7141 cattttcttt tcctccattc atctaatcgt ttttatcgac gttttgttta ctaggcgttc
     7201 ccgtacaata agaacgtgat caggcataag atcgctccga tgatccaacc agttgggatg
     7261 gatttcttca tatattctcg agaagttact tttgtataac ctaatcccca agcaacccaa
     7321 gattgtgtaa tatctaaatg ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa
     7381 aaggctggcg acatcgttgg attaacagct aatacgacag ctaaaatagc tgagcctgaa
     7441 ccgactagat tcattggacc gcggaaaaat cctagtggtg tcaatatagc aaatacgatt
     7501 gccaagacta atggtgattg cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa
     7561 gcatacgcag ctgaattatt gaacatcgct aaggtcatca agaaaccaac cattggcgca
     7621 acatctactg cgccatctgt aaataatttc gcaaactgac gacagatttc tttataacct
     7681 ccgcgcaatt ttccagtaaa cagcaatgcc cagatacctg ctagaataaa acctaagatg
     7741 attggaagat ctaaaagaac gacccctaaa actggtaata atacggctaa ccatgagatc
     7801 attggtgcgt cgccaccttc agctggtacg tttgctgcca ttgcatagcg tttctttttg
     7861 ttcattgata tattagcgac cgttagtacc acaactaagc ttacaatcat cccaatcata
     7921 ccaatttgga aatagtcatt ataagtatag ctttcagccg ctggattgaa accagcataa
     7981 atcgtttgat attgtttgaa attgacgata ttagcaaaga ttccagccat aatcgagccc
     8041 ataaatgaga acagtgcaac cggtactgaa atacctaatg acatcaagat tggcaacaca
     8101 ataactgcaa tagaaatcac tggtccgatt cctgtcatcg acatgaatat aacagcggtt
     8161 acaatacata gtaagctcat cgtgattctt ggcttgtccc ctccaagttc aactaccttt
     8221 cgaatcaggg tagctgcgat tccgctatct actagaacac gcccgaaaaa ggcaccaaag
     8281 aagacattta ccaagatcga tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc
     8341 aacacatcaa ttactccttg cccttccatc gccgggtttg gtgaaaacgt gtttccgact
     8401 agcgctaatc ccatccatac agtagccatg atggcaaagc cgaccatcaa gttgtagcct
     8461 tttacacagt accagaccat gaaaaagaat gagataacta acaaaatacc aataataata
     8521 tcactcatgt tattcctccg tccattttgt ttttctgata agcaaactaa tatatcagtc
     8581 aaactcattc taacttatgt agttagcgct ttcattaaag ggtgaccata aatgttcagt
     8641 tttcaattaa aaaaccttaa ttatgctatt ttagacttat acgttattgt cctgtttcat
     8701 ttgatggagg attgtaaata aaaaaagagg aaagaacata acttttcgtt atcttctttc
     8761 ctctttataa ttgtttagct tatttagcca aagcttcttt tgcttggtca gccaatgcag
     8821 taaatgctgc tgcatcgtta acagccaagt cagctaacat tttacggttg atgtcgattt
     8881 cagcaacttt caaaccgtgc attaattttg agtagctcaa gccattcata cgagctgctg
     8941 cgttgatacg agcaatccac aatttacgga agtcgcgttt cttttgacga cgatcgcgat
     9001 atgcgtagtt gtatgaattc attacttgtt cttttgctgt tttaaataaa gtgtgttttg
     9061 aaccgtagta accttttgct aattttagca ctcttttacg acgtttacga gttactgttc
     9121 cacctttaac acgtgccatg tataattcct cctaataaaa ataaatcttc agattctgtt
     9181 atttgtactt cttatttcat ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta
     9241 gaaaccatgc ttgctttacg caattgacgg cgttgttttt ttgttttgcc gtggaaacgg
     9301 tgacttgtaa acgcgcggaa gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt
     9361 gctgatccgc ggtgtgtttt ttgttttggc atgactatat ttcctcctca aaatctttcg
     9421 ttatccgact atcggctgtc caacagccag ttagtcattc aaattactta ctgtcgtttt
     9481 tcggtgccag cgtcaagaac atgctgcgtc cgtccatttt cgctttttgt tccactgttg
     9541 caatatccgc agtttcttca gctaagcgat tcaagacttt ttgaccaatt tctttatggg
     9601 taatggcacg gcctttgaaa cggatagaag ctttcacttt atcgcccttt tccaagaact
     9661 tacgtgcatt acgaagtttt gtattgaagt cattcaagtc gatcgtagga cttaagcgaa
     9721 cttcttttac attgatcact ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt
     9781 cgaaacggta tttaccgtag tccatgattc gcgcaactgg tggtttcgca ccaggggcaa
     9841 ctaacacaag atccaagttt gcttgttctg caatattcaa tgcttctgct tttgtcttaa
     9901 ctcctaattg ctcaccatct gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt
     9961 taaccatcat atcctttgct atggtcattc acctccaaat attatgagag aaagaaaagc
    10021 ttaccgaata aacggcaata aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc
    10081 taatagaacc agtgaaaact atctagccca gtgacattat caactaaggc gagaagcggg
    10141 tgcttctgct tttatttctc aaccatcata gtatagcaaa aactaatctt tttgtcaatg
    10201 tttttcatac agtttgtctt ccttttctga aaatgattag attagcagac tttctagcag
    10261 tgattcccgc ttctttgtgc tatactaaag aaaaacgagg tgaacccaca tgaaaaaaat
    10321 cgaacctatt ccaccttctc ttcctcttct tacaaaggga gagttttatt tagaagatga
    10381 ggagatcttc tctggtcttc tgatcaacgg gggtgaccaa agctatctcg atgccaaaaa
    10441 tatggtatta agagagagtc atatccaaaa acttacgatg caaaaaacag ttttacgtca
    10501 ttttgaatgc agtaatgtca tttttgaaaa atgtgatttt tccaatctgg agtggattgg
    10561 tggcagtttc caccaagtcg tgtttcatca atgtaaatta acaggtacga attttgccga
    10621 aagttatctt cgagactgta ctttcactga ttgtattgct tcgatggctt cctttagtag
    10681 tacaaacttg aaagctgtct ccttcgatca ttgtcaatta gaagacagcg aattttatga
    10741 ggtcacttgg aaaaaccttt ttttgtctga caatcagttg accggttcga actggtttcg
    10801 aacttctctg aagggattag actttacgac taatttcttc acagcaatcg ctttatctca
    10861 agattctttg gcgggactaa tcgtcaacca agagcaagca cttgtgatcg cccaagccct
    10921 tggtttgcag atcaaagact agatcaacga atccgccgcc acagtgcaat aagcatccaa
    10981 atagataaga ccaaactgat gatcaaggcg attccccatc cgaaaacgga atgggtaaag
    11041 ggtaactgga gattcattcc gaagaatcct gtcacaatag ttggaacggt caagatcagc
    11101 gaccagaccg tcaaaaattt catcgtgtca ttcaagttgt tattcagcaa attattataa
    11161 gtgccagaga gctggtctaa aatctgcgaa gctaagttcg tcatttcaac tgcttgtcgt
    11221 gcttcgatca gcgcatcgtc caactcttct ttttcctttt ctgacaactt gcgataaata
    11281 gccaatgcct tgatttgttc taaaagtact acattttgtt ttgttcctgt aactaagtaa
    11341 accaaaccga tctctaaatc tgaaagttgc agcagattct tgttcgttgt tttttctctt
    11401 aatttcagat taagccgctg acgttcacta ttcacttcct cgattaaagg aaaaaaggaa
    11461 tcagaaatca aaaacaatgt acgaaacaat aaactgtata cactcatgtc tggcattcgt
    11521 tccaaaagcg atcgaattag tcgaatcacg tactcggtat tcggcgtggt aaatgtaaac
    11581 aaaccgtctt cttttaaaat aaaagcgata ggactagttt cataatgatt atcgactttt
    11641 tgctggtgcg gaacatttga tacaagcagt aacgcttcat caaaagaatc gtactctacg
    11701 cgcgcccgtt catttttatc tagagaataa gtcagcatct cgtcactaat gccatattct
    11761 tcttttaagt ttttaacagc tagcacatcg ttttgctgta cattgatcca aaaattacgg
    11821 tcttgattaa aatagtgttt ttcccgcata ctctatcctc ctgacatttg tttccagtat
    11881 aaagaaaaaa gtcgctcaaa taaaggttta ccctttattt gagcaactgt aaatcaaatg
    11941 gctactttgg tttggcgaat ctctgctaat ttttcttcga tcattttcaa gacttcttct
    12001 tttgcttgag gatcttccac aaaattatag cggtctccat cgatttggat ttttggactt
    12061 tctttgtatt cttcatacca ttgatcgtaa cgtctgttca attctttgta ataatcgtat
    12121 agacttggat caaaggacaa ttgttcatac gaacgaccac gtttttcgat acgctccaac
    12181 atcgtatcaa aagatacacg aatatgaact aataaatccg gatgtttttt atgggcagca
    12241 tagggaagtt cttccatcat gttttccagc agttcatcat acacacgaac ttctgtttct
    12301 gttgctcttc ctagatctgc atttagatgg aaaagcagtg aatcttcata aatcgaacgg
    12361 tccaatacat tattgtcatc tttcattgct tgtttgatgc ttgcaaaacg tttattcaaa
    12421 aaataaattt gcagcaaaaa agcatactgt tcagggtttg cataaaataa gggcaagact
    12481 tcattatcat caatcgattc ataaaatgcc tgactatcaa agtgttcagc catcatttct
    12541 gttaaacttg attttccggc accaattgta cctgctaaaa cgatcacgct catttacctc
    12601 cagctcactt catcactaaa tattgtgttt aactccaacc tttttaaata tacccccaat
    12661 atctagtagt gtcaattgta tttttttcta gtcaaagaag aaaaaaagtc aaaaaattct
    12721 taagaaaaaa gtgttttttc tttagaaaac gtattcttag ttttacttct gagatggttt
    12781 tagtaaaata aaggaggaat gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc
    12841 agcgcaactc gttttgggcg ctttattttt tgcttaaaaa tcacttttac gtgagaagga
    12901 gttagattat ggcaaactat ggccaaggag aatcaagcgg aaagatcata ttgatgggcg
    12961 agcacgcggt tgtttatgga gaaccagcga ttgcctttcc tttctatgca acaaaagtca
    13021 ccgcattcct tgaagagctg gatgcaatgg acgatcaact ggtttcttcc tactattcag
    13081 gaaatttagc cgaagctcct catgcattaa aaaatatcaa aaaattattc attcacttaa
    13141 aaaaacagca tgacatccaa aaaaacttgc aactgaccat tgaaagcacg attcctgctg
    13201 aacgtggaat gggatcaagc gctgcagtcg ccacagcagt cactcgtgct ttttatgatt
    13261 acttagcatt tcctttgtct cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa
    13321 tcgcccatgg taatcctagt ggaatcgatg cagccgctac tagcagcttg cagccgattt
    13381 attttacaaa agggcatcct ttcgactact tttctttgaa catcgatgct tttttgattg
    13441 tcgctgatac aggaatcaaa ggacaaacaa gagaagccgt caaagatgtc gctcacctct
    13501 ttgaaaccca gcctcatgaa actggacaaa tgatccaaaa attaggatac ttgacgaagc
    13561 aagcaaaaca agcaatcatt gaaaattcac cagaaacgtt ggcacagaca atggatgaat
    13621 cacaatcact tctggaaaag ctgacaatca gcaatgattt tcttaatcta ttgatccaaa
    13681 cagcaaaaga taccggggca ttgggtgcca aattaactgg cggtggacgc ggtggctgta
    13741 tgattgcatt agcacaaaca aaaacaaaag cccaagaaat cagtcaagca cttgaagatg
    13801 cgggtgctgc tgaaacttgg atacaaggat taggagtaca tacctatgtt taaaggcaaa
    13861 gcacgcgcat ataccaacat tgctctaatc aaatattggg gtaagaaaaa tgaagaactc
    13921 atccttccaa tgaacaacag cctttcgtta acattggatg cattttatac agagacagaa
    13981 gtcatctttt ctgatagcta tatggtagat gaattttatc tagatggcac cttgcaagac
    14041 gaaaaagcaa caaaaaaagt cagtcagttt cttgaccttt ttcgtaaaga agccggcctc
    14101 tcactaaaag cttcagtaat cagtcagaat ttcgttccta ccgcagctgg attagcctcc
    14161 tctgccagcg ggctagctgc tttagcaggc gcttgcaata ctgctcttaa gcttggatta
    14221 gatgatctct ctctttcaag atttgctcga cgcgggtctg gttcagcttg ccgaagtatt
    14281 ttcggtggtt tcgtcgaatg ggaaaaaggc catgacgact taagttctta cgctaagcca
    14341 gtcccttccg attctttcga agacgattta gcaatggttt tcgttttgat caacgaccag
    14401 aaaaaagaag tgtccagcag aaatgggatg cgtcggacag tcgaaacatc cagtttttat
    14461 caaggctggt tagattccgt tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca
    14521 aaagatttcc aacttctcgg agaaacgatg gaaagaaacg gactaaaaat gcatggaacg
    14581 actttagctg cccagcctcc attcacttac tggtctcctg acagcttaaa agcgatggat
    14641 gcggtgcgac aattgagaaa gcaaggcatt ccatgctact ttacgatgga tgctggccct
    14701 aatgtcaaag ttttagtgga aaacagccat ttatctgagg tacaagaaac atttaccaaa
    14761 ttatttagca aagaacaagt aattactgca catgctggtc caggaattgc gattattgaa
    14821 taaagggaga tttttatgat tgaagtatct gcaccaggca aactctatat tgccggagaa
    14881 tatgcagttg ttgaaacagg ccatccagca gttatcgctg cagtcgatca attcgtgaca
    14941 gtcactgtag aatctgcacg aaaagtcgga agtatccaat ctgcacaata tagtgggatg
    15001 cctgtacgtt ggacaagacg caacggagaa ttagttttag atattcggga aaatcctttt
    15061 cattatatcc ttgctgctat tcgcttgact gaaaagtatg cacaagaaaa aaacattctt
    15121 ttatcctttt atgatctgaa agtaacaagt gagttagaca gttcaaatgg ccggaaatat
    15181 ggattgggtt ctagcggtgc cgtgaccgtt gcaacggtca aagccttgaa tgttttttac
    15241 gcgttgaatt tatctcagtt ggagattttc aagattgcag cactagccaa tttagcagtt
    15301 caagataatg gttcctgcgg cgacatcgct gctagctgtt atggtggctg gatcgctttc
    15361 tcaaccttcg accatccttg gctccaagaa caagaaactc agcattctat cagtgagtta
    15421 cttgccctgg attggccagg tctatccatt gagccattga ttgctcctga agatttacgt
    15481 ttattgattg gttggacggg tagccctgcc tctacttctg atttggtcga tcaagttcac
    15541 cgttcgagag aagataaaat ggtggcttat cagcttttct taaaaaatag tacagaatgt
    15601 gtcaacgaaa tgatcaaagg gtttaaagaa aataatgtaa cgttgatcca acagatgatt
    15661 cgaaaaaacc gacaattact gcatgattta tctgcaatca ctggggtcgt catcgaaacg
    15721 cctgctttga acaaattgtg taatttagct gaacagtatg aaggagccgc aaaatcttct
    15781 ggtgcaggtg ggggcgattg cggaatcgta attgttgacc agaaatctgg cattcttcct
    15841 ttaatgagtg catgggaaaa agcagaaatc actccactgc cgttacatgt ctatagcgat
    15901 caaagaaagg aaaaccgatg aatcgaaaag acgaacatgt ttcactagct aaagcctttc
    15961 atgataaaca aaaaaacgaa tttgacttcg tacggatcat ccataaccct ttgccgcaaa
    16021 tagcagtttc tgatgtcgat ctaagtacac aggcagttgg gtttacacta agcagcccat
    16081 tttatatcaa tgcgatgact ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg
    16141 caatcgtcgc aagagaagca gatttgatga ttgccactgg ttcagtcagc gctgccttga
    16201 aagtcccttc tttagccgat acttatacga ttatgcgaca agaatatcct cacgggaaaa
    16261 tcattgccaa catcggtgca gggacttctg tcgaaagagc caaagaagct atccgattgt
    16321 ttcacgcaga tgccttacag attcatttga atgcacctca agagttggtt atgcctgagg
    16381 gagaccgtga ttttaccaat tggaaagtac ttattcaaga aactcaaaca gccattgacg
    16441 tccccctcat cgtaaaagaa gtcggttttg gcatgacaag agaaacgctc aatgatctag
    16501 ctgctttagg agttcataca gttgatatca gcggtcgaag cggaacaagc ttcacccaga
    16561 tcgaaaacgc ccgtcgctcc aagcgggaac tgagttattt agctgactgg gggcaatcga
    16621 cagtctcttc attgcttgaa gcaaatgaag cagacacttc tatggaaatc ctagcctccg
    16681 gaggtatccg caatgcctat gacatattta aagccctttg tctcggcgca aatgccgtgg
    16741 gtacttcagg aaccgtactg acgcatctga tgaatcacgg cgtagaagaa actattatac
    16801 tgatgaagca atggcaagaa gagcttcgtc tgctttatac tatggtcggc gcaacgaata
    16861 ccgcggccct acaccaacaa tctctgatct tttccggacc agtgaaggat tggtgcgaag
    16921 caagagggat cgacttagtg aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt
    16981 aaagaacact tttgtctcac gctctaaaca cgtacaaacg gcggaagtaa aagcaactcc
    17041 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa ttccttctta
    17101 tttcttggag ttaaatgctt ctgtcccagc ctcttttttc gaataaatga tgtttaatca
    17161 gctgtccttc ggtattcgtt caatccgctg atttcacaac ctctccacta aacagtgtac
    17221 taaagctctt ttaaggttgt tttccgatat aggcaagtat gcctccgtcc acatacaaga
    17281 catggccgtt aacgaaatca gatgcgctag aagccaagaa gacagatggt ccagccaaat
    17341 cgattggctc gccccagcgt gctgctggcg tacgtccaac gataaattga tcgaatggat
    17401 gacgttcacc gttttcttgt gtttcgcgta atggagcagt ttgcggtgta gcaatataac
    17461 ctggtccaat tccattacat tggatattgt attgaccgta ttcagaagcg atgtttttcg
    17521 tcagcatttt taatccgcct tttgccgcag cataagcact gaccgtttcg cgtcctaatt
    17581 cactcatcat ggagcagata ttgatgattt taccgccgcc tttttcgatc atatccggaa
    17641 taactgcttt tgaaacgatg aatggggcat ttaaatccac gtcgattact tggcggaagt
    17701 ctgccgcatc catttcgatc atcggtgtcc gtttgatgat ccctgcattg ttgactagaa
    17761 tatcaactga acctacatct tctttgatct gttgaaccat cgcttgaacc gcttcttcat
    17821 cggtcacatc gcagacataa ccttttgcat cgatgcctgc ttctttataa tgcgctatac
    17881 cttcatcaac tgattcttga ttcaagttat taaatacgat ttttgctcct gctgtagcta
    17941 aagagcgtgc aatctcaaat ccaataccat agacagcacc ggtgactaat gctacttttc
    18001 catctaaacg aaacatatcc atactaaatt ccatcatttt ctctccctta tgcttatttt
    18061 aattgatcca tttgcaccat atccatgtct gtataggtga tattttctcc gcacattgcc
    18121 cagatgaatg agtaattgct tgtacctaca cctgaatgga tcgaccagct tggcgaaata
    18181 atggcttgtt cattgtctac aactaaatgt tttgtttcat ctggtttgcc catcatgtgg
    18241 aacacacgtg tatcttcatt ggcataatca aagtagacat aagcttccat acgacgttca
    18301 tgtgtatgac aaggcatcgt attccaagcg cttcctggtt ctaaaatcgt ataacccatt
    18361 tgtaattgac agctttcgca tacatttgga tggatgtatt ggtagatttt acgttcattc
    18421 agtgtcaggc cttcaccagt ctccattggt tttacttggt cgatactgat ttttacattt
    18481 ggatatttat gatgagctgg aacagaacta atatagaatt tagctgggtt ttcagcatct
    18541 tgagaagaaa atacaacatg tcgtgtttct tttccgatat agtatccgtc ttgttttttc
    18601 atttcttctt tttgcccatc gatctcgatg aatcctggtc caccgatatt gatcacacct
    18661 agttcacgac gctctaagaa gtaatcaaca cctagttctt tgtctaatgt gatttctaat
    18721 tctttttcag taggtgtcac tccaccaaag atcattcgat cgttgtgcgt ataagtcaaa
    18781 ctgattttcc ctggttcaaa taccttttct actaaaaatt cttttctcaa ttgttccgtt
    18841 gaataatgac ggatatcttc tggactgtgt gtataacgtg tttccatttc ttgcattttc
    18901 ttctactcct tctatcgaat gatttttcca gagccgcatg ctaaaaattg tgcgacttct
    18961 tcttgactaa attggttgca gtccccatgg atcgtatgct tcaaggcaga agctgccgta
    19021 gcaaaatcaa tgatttcttg cggagacata ttggataaaa tcccatggag cacgccacct
    19081 gcaaaagcat ctccgccacc tactcgatcc acgattgggg tgattctatg gacttgggat
    19141 tccacatatt catggttcat ccacaatgtc cccttcaatt cattttcact tgctgaatga
    19201 acggtacgct tggtcgaata aaacacctga atattcggga acttttcctg cattttttga
    19261 taataataaa ttagaggttg ttcttctgct tctggcgctt tctcgatacc taacaaataa
    19321 agagcatcca tttttccagc tgagcagata tctactaaag gtaaaagctg atggattact
    19381 tcgctggctt cttgctgtgt ccataatttt ccacgatagt tgatatcgaa gctgatcagg
    19441 cagccagctt tttttgcagc ttctatcacc tgtttcgtca ttttttgcca ttttttagac
    19501 aaagctgggg tgattcctga aacatgaaat atatctattc cttggaataa ctcctctagc
    19561 gaccattcca aatgatccat ttcagcaaaa cttgatccgg cacgatcata gatcactgag
    19621 gcggcgcgtt cgccactacc agtttccata taataagtac ctagtcttga tccaccagat
    19681 aatacctgtt tcgtatctac tccataacat tgcaagtgct ttttgactgc ttttcccaat
    19741 gcgttatcag gtacttttgt agcaaaaatc gcttcatgtc cgtaattagc taaagaaatt
    19801 gctacgtttg cttcccctcc gccataatgg gcagcgaatc gttcacttga atcgatacgg
    19861 gttccctgct ctgtggacaa tcgcaacatg atttctccga gtgtcactac ttttcccata
    19921 agcagctacc tcttgatttc tttcattttt gctgcatact gttgcgcgac ttccgttact
    19981 ttgtcaaaat ctcctgtagc tgcgggtgct aaaaggttcc cacctactcc gactgttacg
    20041 acaccagcat cgaaccactc tttcatattg tccagactca caccgccagt tggcatgata
    20101 ttgacttgag gcatcggcgc cttgaaggca gaaatgatac ttggtccata agcgcttcca
    20161 ggaaatagtt tgacgatatc taccccgctt ttcagcgctg ttttgatctc ggtaatagtc
    20221 atacatcctg gtaagtaagg aatttgatac agattgcaca tctctgccgt ttcttgatca
    20281 aaggaagggc taacaatata ctctgcgcca gctaagatag ccaagcgagc tgtgacagcg
    20341 tctaatactg tgcctgcgcc aatcactatt tctggttggt ctttgtagaa tgacaaaagt
    20401 tctttgatta cttgatctgc ttgagggact gtaaaagtca actcaatacc agttagacca
    20461 cctttgatga ttgcgtggca agcattcaaa gcttcttctt tgctttttcc gcgtaccacc
    20521 gcaatcactc cggcgttttt caatcgagat aaaatatcaa tttttttcat cttctccacc
    20581 tatttcctat ttagaaattt tattttataa cttggaaatc tatacccata ttatacggca
    20641 gaaaagaaaa attgcaaacg cttttttcat tatttttgaa ttctttgctc gatttcgctt
    20701 tttgtttgct tgagggcact gattatttgt tcttttgtct catctgttaa acgatatttc
    20761 ggcatactta cactaaatgc accaatgatt tgttcgtttt tcacgattga ggctcctata
    20821 caaaaaatat cacgttcgat ttcttcatcg tcaaatgcga tgtgggtttc ttttaccgtt
    20881 ttgatttcct ttttcaaccg taatgcattg gtgatcgtat attctgtgta agggatcaac
    20941 ggatgctttt caagataatc agtgtactgc tcttcactga attctgctaa tacggctttg
    21001 cccatcgcag aattgtataa tggtcgggtg attccgattt ttgaagacat gcggatcgtt
    21061 tgatgttttg gatctagttt gttcacgtac aagatctcat tgttgtccaa tacccctaag
    21121 tggatagtct catcgatctt ttgctgcagc tgttctaaaa acggtaaagt caactctacc
    21181 aaatcgattt gttcaatgtt tttattcgcg tagcgaataa gctttgcacc taaacgatag
    21241 tttttctctg aatttttatt tacgtagccg atcatcaaca aagtatctag tattttcaag
    21301 actgtagaag aggtcattcc cacacccttt gcgatcgtct gcaaagagac atcttggttt
    21361 tccgctaaat agtctagtat tttagaggct ttgatcaata cagttccata aggcttttta
    21421 ttttccatat aatattctcc tatttcttta taagaaaaat tatagcggaa ataccatcaa
    21481 tttaaaagag atatccagat ataaactacc atttctcttt acaggcaatt taatgttgaa
    21541 gatccgcttc gtttttacat caaactttga tataaagcaa tgatttcttc tactgtcgtt
    21601 tctcttggat ttcctggtgt gcatacatct cgcaaagcat cttctgcaat tgctggaata
    21661 tctgcttctt ttacacctaa ttcagaaatg gtttctggga ttccaaccgc tttgctcaac
    21721 cgatcgatct caccacaagc cgcttctctt acatcttcta aagacatttc agctgctcct
    21781 ttaatcccta gaacaagagc gatctcacgg tacttttctc cagtatattc tttgttatat
    21841 ttcatgacag ttggcaaaag tgctgcacac gctacgccat gaggaatgtt gtaccatgca
    21901 cttaacgggt gagccatccc gtgtaccagt cctaaaccga catttgagaa gcccattccc
    21961 gcaatatact gtcctaatgc cattttttct cgcccatttt gatcgccatc tacagaagct
    22021 tccaaagaat ggccgatgat ttcgattgct tttaaatgca gcatatcagt catttcccaa
    22081 gcgccttttg tgataaaccc ttcaatagca tgcgtcatag catccatacc tgttgatgca
    22141 gccaattttt tcggcattcc catcatcata tcgctgtcga tgaacgcgac gattgggata
    22201 tcatgaggat caacacaaac aaattttcgg tgatttactt catctgtgat gacgtagttg
    22261 atcgttactt ctgctgctgt tccagaagtc gttggtatag ctaatatagg taaacatgga
    22321 tttttcgtat cagcaacacc ttctaaacta atgacgtctg aaaactctgg attcgtcaaa
    22381 ataatcccaa tggcttttgc cgtatcgatt ggcgaacctc caccgattgc gatcaaacag
    22441 tctgcctctg cctctttaac aaaagccact ccatctttta cattttggat cgttggatta
    22501 ggtacgatcc cgtcgtataa agcataagcg attgctgctt tatctagaag ttctgttact
    22561 tttgttgcta taccgtgctc gatcaatcct ttgtcagtaa tgacagctgc tttgccgaat
    22621 cctcgctgct gaaattcagg gacgatcgac tcaatcgctc cttttccaaa atacgatgtt
    22681 tcatttaaaa tcattcgatt actcattttc tattcctcct aatttgtatt atttttcaca
    22741 ataaaaacga aagaacccca agcaaccggt tgcccagttg cttgttgcta cagttcaaag
    22801 actggcatca attcccaact gactggagag gcatctggat ttgtctccat gatatcttcc
    22861 atgtacttcc accatttttg attgatagat gtatttgcca ttttttccca taacttttca
    22921 tcttcgatca ctaagtaacc aaataacaaa cttgtactcg gatcaagata gatcgaatag
    22981 tcgatgaccc cgtgttcacg aagtgctgta cgcatttctg gccaaagttc atcatgtctt
    23041 tttttgtatt cttcttgttt ttctgggtaa actttcatgc aaaaagcttt tttcaccatg
    23101 tcagacacct gctttcgtat gaacttcctc caaataacct gcttttggag tgacaccaaa
    23161 cgcatctgct aaatgccaca ggttttcgtc cgtgattgtt tgcaagattg gtccttgcgc
    23221 tttgacgtac gtatagactt ctgctgcctt ttctgctgtc tcgatcaaac caaagacttc
    23281 atccatgtct tttcctgacc cgtagattcc atgttgtggc cataaaacca atcgagtttc
    23341 tttcattttt tcagctgttg cttccccaat ctcatttgtt cccggaacca tccacggaat
    23401 gatggaaatt ccttctggaa aaacaactaa gcattccgta cacatttgcc atagcgtacg
    23461 actaaaggaa cgttcgtcta attcatgcgt aaaactcatc gctaatagat gactagcatg
    23521 gcaatgcata acgatccgat tatctgggtc tacttctaaa cgggcgatat gactcatgaa
    23581 atgacttggc aactcgcttg taggaaggca ttcgttttcc agcccccaca aaagttccag
    23641 tgttttgcca tcttcagcaa ctcgaacgat tccaagattc tcggcaggtt cgctcgctac
    23701 atttttaaaa tatcttcctg aaccagttac aataaaatag catcctgcta aacggctggc
    23761 atcaaaaatc atcgagatgc ggcggatcac tttttcagga tccaagaacg gtgtgatctc
    23821 ttcttctttt agtaaataag aaatattccc gccatttctt tcatcccatc ccaagcgata
    23881 aagattcgac gtcgtttcga tcatttcttt cacataaggc gcttgtaaaa tatctatttt
    23941 tttcaatata gccacactcc tattttctta attccaacac gtctttttga tattcctgga
    24001 cttcttccag ccaatccaaa ccaactggaa cttgattttt gtaacagaaa taattccata
    24061 catcggcata cgggaaatct ttcaattctt ccgtataagc cattcttgaa gtaaagtcaa
    24121 aagccagctc catttctttc aatcgctcaa ctggttccag catagctttc agcagtgctt
    24181 tttgtgtatt tctcgtaccg atgacccaag cagcgatacg gttgatcgtt gcatcaaaga
    24241 aatccagacc gatatttgtt ttttccaaca attcgttacg gactaattct ttagcgattt
    24301 cctgcaattc atcatccatc aaaaccacat ggtctgaatc ccaacgcact ggacgggaaa
    24361 catgcaacat gattcctttg ctgaataaag ctaatgaaga taatttattg gaaatcactt
    24421 ctgttggatg aaaatgtcca gcatctagac aaacaagttt gtcacgagtc aacccgtaac
    24481 ccatataaaa ttcatgggaa ccgactgtat aagcttcagc gcctaaacca aatagtttgc
    24541 tttctactgc atcttgtaca taccgttcat ctaactcttc tgtaaaaatt tcatcgagtg
    24601 attccattaa acgttcccgt ggggtaaaac gatcaatcgg attatctttc atcccatcag
    24661 gtacccaaaa gttattgata cttgtttgcc cagtttcttt cccaaagtac tccgcgattt
    24721 tgcggcttct tttcccgtgt tcgatccagt attcacgaac ttctttatct ggatgagcca
    24781 atgtaaagcc gtctttcatc attggatgcg aaaagaaagt cggattgaag tcaagaccga
    24841 tttcttcttt ttttgcccac tctacccatt tttcaaaatg tttcggctca atttcattta
    24901 gatcgacaga ttcttctgta tccaaataga tagcatgaag gttcaatttg tgtttgcccg
    24961 gtatcaacga ataagccttc tccagatctt gtctaagctc ttcaggtgtt cgtgcggctc
    25021 ctggaaaatt ccctgtcgcc atgattccac cggtcaattc gccatccggt gtcaagaaac
    25081 ctttgacatc atctccttgc cagcaatgca tagaaatttt gatgtcctgt atcttttcta
    25141 gtacagcatc tgtatctaca ccgatagttg catatttttc ttttgcttgt tcgtaatttc
    25201 tttcaatcgt gttcatacga ttactccttt gattttattc acgtaccatt ccatcgtgtt
    25261 ttgcttattt tcatctgggt aaaaagtttc acatggaaac gactgtttga ttacatttct
    25321 tgcttgttct atattcgaaa actgacctgt actgagcatt tgcatggcaa cattcccgat
    25381 tgctgtggct tcactaggtc ctgcttctac aatgattccg gcataatctg ctgtcaattg
    25441 attcagtaga cgattattcg atccgccccc tacgatgtgc agcttttcca atttcgttcc
    25501 agttaagtgt tctactttga tcagctcttg tgcgtaacaa agagcaagat tatcatacac
    25561 acaacgagcg atagctcccg atgagacagg gactggctgc tctgtttcaa aacagtattg
    25621 ctgaatctct tctatcatat ttgggggatt caaaaatcga gggtcattga tttccatcac
    25681 ttgctgcagc ggtttttctt ttgcagcaag ctctgccaat tccgcataac tgtactcgta
    25741 ctcttgcatt cgggcaactt cctggatcaa ccacatcccc atgatgtttt tcaaaaaacg
    25801 caccgtgtta tgggcacccc attcattcgt ataattttca gaaaaagcat tgcgagaaac
    25861 attggcgatc tttgtttcaa tccctaaaag cgaccatgtc ccactgctga tataacccca
    25921 acgttctcct tgacctggcg ttcctaatac agctgacgcc gtatcatgac ttgctactgt
    25981 gataaaggtc acatctggca aatcatattt tgaaaattgt tctttttgca gatctcctag
    26041 tatcgttccc ggctctgtca atggtggaaa taattccttc gagacaccga ttttttcaag
    26101 aagttctatt tcccattctt ttgtccctgc attcagcagc tgcatagtag atgcattagt
    26161 tttctccgtc accatgttac cagtaaagac ataccctaaa taatcaggta tcagtaacaa
    26221 tttatgtgct tttgccaaat tttctttttc ttcagcaaac aattggaaca aggtattgaa
    26281 aggctggatc tgaatccctg tctgctcata caacttatct agagaataga tttcagaaaa
    26341 ctgttctact gctccattgg tacgcttgtc ccgataagca atcggatcat ttatccgatc
    26401 cccttgttca tcaatcagac agtaatcgac tgcccatgta tcgatccccg caaaaaggcg
    26461 atcaatccct gtctgcttta atttttcaaa tccaatcaaa atctctttga taagataatc
    26521 gatcttccag cgatcgtgac cgtctttgtt ccgaaagccg tttttgaacc ggtgtacttc
    26581 ttccaagatc aaagaacctt ttgcatccct cgtacttttc attagtcgtc cgcttgatgc
    26641 accgatatcg atggcaacaa atgttggatt catttctcca cctcttttac tttattgttg
    26701 attagcactt tattaataaa gcgttatcat ttattgacaa tgctatcata atacagaatc
    26761 ttatattttg cttcacacta ttttggtata taattgcatt atcttgacaa gttgggagtg
    26821 tttacatgga ttactgggaa aaagccaacc actcatggtc ggaagactca aaaagagtga
    26881 tcttgacgcc tacacaaaga agcaggaatt tattttttta tatccaagaa atcggttact
    26941 tcaaagcacc aatcccttac tatactgaac gagcacattt accttcttac ttgataaagt
    27001 ttactttgtc tggcaaaggt tcgcttacat ataatgataa tcagcaacag ctaaaagctg
    27061 gagacttatt ttttattgac tgccaagaat atcagcgtta tcaaacgatc agcgaagaac
    27121 catgggaaat ggattggatc catttgaatg gtcagaacgt ccaatcattt tatcaggagt
    27181 tcacaaaaga cggccataac accttccatt caacaggaga tccatcagaa aacaagatcc
    27241 accatttgat cacagaattg atccacatgc aacaaggcac gaatgcccgt accgatttcc
    27301 agtcctctgt agctatccac gagttgctga atgaactgat tttgcaaaag tatcagctag
    27361 atttcgaaga agaggatatt cccgcctacg tttacaaact gaagcaatac ttagacgaac
    27421 atttcaaaga aacgattaca cttgatcaat tagaacaaca attcttattg aataaatatc
    27481 agctgagcaa agatttttcc aaatttatcg gttctccacc aatcgattat ttgatcagca
    27541 aaaaactttc ttacgctaaa gaccttcttc ggtatacaga atttacggtt cgaatgattt
    27601 ccgaagaaat agggattgag aattttgctt actttagccg cctgttcaaa gcaaaaacag
    27661 gactatcgcc gagagacttt cgtaaattca gctgattcat ataatatgta cgtcattttt
    27721 tcaaaatatt ttgaaatata tagataaaaa taaaataacc gcagtttctg cttctatgta
    27781 aacaacagca gctactgcgg ttatttttta gtgatcattc tcattgttca tcttcaaaat
    27841 gttcataatg aaccaactgt ttctgtgtat cacgtttcaa aaattcttta ggcgttatgc
    27901 cgaactctct cttgaatgct ttcagaaaat gcggataaga agaaaaacca cttttactat
    27961 atacagcact agagctgcgg tattctcgca acaagtgctt ggcatagatc agctttttct
    28021 tcattacata ctgatgaaac gtaaatccag tgtacttttt aaattctcgt gtgatatggt
    28081 atttgctgac aaaaaactgc ttttccattt cctctaaaga aagcggatga gataaatttt
    28141 ttgagatata ttgtaacatt tgttccacac gctcattagg aactacagca ttctgttctt
    28201 gcgcactctc ttctttttgt acaacttgat tgagaaaaat caagtattct accagagctt
    28261 gttcatattt tatgtcttca ccgtaaattt ttttatcagg aggatggtcg ataaatgata
    28321 atttttgctt caatacttcc tgttctattt gcaatacacg actcctcttc tctcccattg
    28381 gttgaaaaca agccgataga ttagaccatg gcgtggaacg actttctaga aaagaagggg
    28441 tgacaaagat atagacacgc tcgaatacat ctgaagattg gcggacgata cgatgcaaat
    28501 cattggaatg gatgagcagc acacttcctg ctttgatcgt aaattgtcgt ccgttcaaat
    28561 aaaaagtcgc ttctcccttt aatgtagcat ggatctcata gaaatcatga tgatgataga
    28621 tcgttttatt ttggctgacg acatctttga catgatagat ttcatactga tcagaaacca
    28681 tcgtattgac ttgttcaccg ctgattttct tttggagcat gtgccgcacc tccttttgtt
    28741 cacttatgca caattttatt ataccacgtt ttttgtaatc aaaaagagct gcttttatat
    28801 actcttataa aaaagaagag acgtggcatc ttctagcccc gccccctcca ttctaaatat
    28861 tagttaacca acgtcttctt tacattattc aagaagactt cttccataac gaatcccatc
    28921 atcttttgtt tcttcaagaa ccacatacag gtacggacga ttcttcatga tgatttcttt
    28981 gatttttgtg tactcgatca caccgtctcc caaatttacc ggcacgattt tttcctgttc
    29041 gacacgaaaa tctttcaaat gaaccgcaca tatcttttca ccaaaacgtt caaaagcttc
    29101 ttctaccaat tggacttgtt cttgataatc actacttgtg atcaaattgg tcggatctaa
    29161 aatgattcct agatagtccg aatctactgc ttggatcagc tgctctaccc ttgctaaaga
    29221 atacaccgga tgattcaaac ctggttctat ccccaccatc atttggtatt tttctccagc
    29281 ttttaccaag cgacggataa cggacacagc ttcagcaaat gcttcatctg tgaaattttc
    29341 ttccgtatat tggatttccg gcaaaacaca tccagtttcc gaagcaacca tcgacgcacc
    29401 aaaggaagaa gcatagcgaa gatactgctc gaactttgtc agcaactctt gtcgtatcgt
    29461 taaatctgga tgaatcatat tgatatagca acttaaaatt cctacggtca cttcttgctt
    29521 ttctaaaact cgtttgacat aatttcccat cccagaattg atctcatttg cgccagaagg
    29581 tatttcagga aacgaaagac ctaaggcgag ctgcagtgta tgaatccctt gttcttttgt
    29641 tttttctgca agatcttcta tcgtttggac ttcggaaaga tcatgtcctc taattgtaat
    29701 gttcaacata actattctcc taactcagca tattgggtag ccacatgacg atttgcggga
    29761 agtaagtaat caaaaacaag acgactaaga taaccccata aaaaggaaga agcggtttca
    29821 gcattgcctc tgcttttacg cctcctacac ttgcaccgac atatagtcct gtacctactg
    29881 gcggcgtgat ggagcctaca cacagattca tgattgaaaa caacccgtaa tgtacaggat
    29941 caacccctac acttgtgacg attggtaaaa atattggtgt aaaaatcaag atagcaggcg
    30001 ccacatccat aaacataccc accagtaata acacaagatt gactaataac agaataataa
    30061 atttattatc cgttacgccc ataatcaacg aactaattgc ctgaggaata ccggtaaacg
    30121 ccatggcgaa agacatcatg gaagaagtag ccaataagaa catgatcgtc cctgacattg
    30181 ctactgattg aaacaacata ttaggtatat cttttagttt taccgtttta tagtaaaaca
    30241 tagcaatcaa cagggagtaa atgacagcta tcgctgaagc ttctactgct gtaaatatcc
    30301 ccgtcaagat accaccgata atgatgacaa tcaatagaag gctaggaatc gcctctttta
    30361 ttatttttcc agcatgagcc atctgtcctt tttcgacaac agggtattta ttctttttag
    30421 caacgatgaa tgctactagc atgattccta gcgcccacaa agcgcctacg agataacctc
    30481 ccataaacaa agcagagatc gaagcaccac cagcaacaag tgaatacatg ataaaagcag
    30541 tacttggagg aatgaccata cctgtcggag ctgacgcgat attgaccgct gtcgcaaact
    30601 tccggtcata tccttcttcc acttgctgag ggatcaacac tccgccaatc gcggtagaag
    30661 cggcaatggc cgagctagag atggaaccaa ataaagcatt ccctaacaca ttcgtatgag
    30721 ccaaagcccc cggcacgcgc ccgacgaaca acatcgcaaa atccactagt ttcttagcta
    30781 tccctccatt attcatgata atgcccgaga gaacgaaaaa cggaatagcg atcagcgaaa
    30841 aactattgat acttcccacc attttttgag ctgaactaaa agcagaaaca tcaaacggta
    30901 taactaacaa taaagtcatc atcgatgaga atgcaatact tatagcaatc ggcatcccta
    30961 atacaagcaa cacaacaaat acaaaaaata acactaatcc ggcttctatt gccattttat
    31021 tcctccattt ttatattttt tcttgaattg ctggcttgtt gcttaatgta tcgattagat
    31081 tgatcaggct atagataatg atgagtaccc ccgatacagg tactgacaga taaacatatc
    31141 ccataggaat acttaaagaa ggcgagatct gccccattgt caatgtgaca gcattccatc
    31201 cgccatacac catgatcatc gcagcaaata ccagaaataa actttgaaca acaacttcta
    31261 aaattttact ttttttgcca gttaattttt cacttaaaac cgttacggct aaatgtgctt
    31321 tttgtcccac aacatacgcg gaagacaaca tggcaaacca gacaagaccg aatcgaacaa
    31381 gctcttccgt gaccgtattt ggatttccta aaaccgttct ggaaaatact tgccataaaa
    31441 caacagcgac catcccaaca agcaaaacag ctcccaggat ctctaatacc ttgtttaaaa
    31501 tcattttcat cacagctacg ctccttgctc ttggatcttt tgataaaggt ctttgtaggt
    31561 aggatcgttt ttgaatatat catgaagtgg ggcaactgcc tctcgaaacg gtgtattatc
    31621 tacatcattg aattggactc catgttcttc ttgtgctaaa gctttttctt cttcaattgc
    31681 ttttgcaaaa gcttcaattt caaaaaccgt tgattctttc gctgcttctt caatggcttt
    31741 ttcctgctca tcagttagct gttctttgat ggcatcattc atgatgatga tatctggcac
    31801 acgtgtatgc tcatcatagg aatagtattt agccacacca ccatgtccag ctgtatacaa
    31861 gacaaattca ttgttttctg atccgttgat caaattggat tgaagagacg tgtacacttc
    31921 atcacttccc ataggtacgg gcgaaccgcc taaaagctct accattttga tagcggtttc
    31981 gctttgcatc acacggatct tctttccctt caaatcttcc ggcgtatgga tcggaccgtc
    32041 taccatataa aagctgcgct gaccagaatc ataataggtc aatccggtaa atcctaaatt
    32101 tttggtcgat tggtagattg gctccattat ttcttcatta tccatcacct taaagaaatg
    32161 ttcttcatca ttgaatagat aaggaactga aaaaatcgaa taatctttag agaaactttc
    32221 caacgctgag gcacttactt ttgtgaaatc tactgctcct gactgtgtca attcgatcag
    32281 ttcacgttct ccacccagtt gtccatctgg gaaatactgg atctgtacgg aaccatctgt
    32341 tttttctttt actaaacgcc caaattcatt cattgcatca attactggct ggctgttgct
    32401 tgcataagca aagcgcaagg taactatctc atttacatct gcatctgcga cagttgccac
    32461 actagaatta tttccgcagc cgcttagacc tagcagacct aggctaagcg aacttgctgc
    32521 aaacagcagt gatgtttttt tcatatgggt ttcctttctt ttaacaataa gagacgagca
    32581 attctgtcaa acaaagcacc gttaacgact gtccataagg cattgctgtg attccgatat
    32641 tccgataaaa atctaaactg tcccccattc ctgtcccgat tgaaacgttt tgaacttctc
    32701 ctttttcatc gatttgttca agcaatcctt ggatagcacg ataagcgact tctttatatt
    32761 cttgtggtaa ataacgttta tggaccgctt tcaaaatacc gtaagcaaat cctgcagttg
    32821 cagaagattc tacgtaagaa gaaggatcat ccaacaacgt atgccataaa ccgctttcag
    32881 actgataagt ttttaatgca cgcacttgtg cttcaagtgt ttcgatcaat aatttgcgca
    32941 atgcatcttc tttcgataat tctaagattt caatgatctc tggaatagca atcgtcaacc
    33001 agcagtttcc acgagcccaa aatgcttcgg catagttatg atttccttca aacgtccagc
    33061 cgtgatacca caatcctgtt cgtttatcca tcaagtattt gatatggatc ataaactggt
    33121 aacgagcttc ttctaaatat tctgggcgat tcaacagttt cccgattttt gccaaaggaa
    33181 gcactgtcat catcaatgta tcgtcccaca actgattctt attttccggt ccatatgtgg
    33241 catgctgtaa cccgtcttca tttgtccgag gcatgtcatt catgacccat tctgcccatt
    33301 gttctaaata aggcaaatag cgtgaatctt tcgtgtcttc atacaagtaa gccatagtca
    33361 atagcggtgc cattgtattg acgtttttgg gcggagcgcc ttcttccata cgatcctcaa
    33421 accattcatt gaccacttga taagcgcgag gattttttgt catctggtaa tttttataaa
    33481 taccatatag accgacacct tgtggccaat tccacactgt ccagcttttg tcatctactt
    33541 tcagtccatc aaagtccaac aaaaattcac cattatcgtc tttgatcgtt gtcaggtttt
    33601 caacgagcag gtcaattttt tctttcacta attctttagg tacaaaaatc tcttctttaa
    33661 taatcatctt tacgccacct ttttaaacgc tttcatttta tatccttaat atagcagtaa
    33721 taccgtccac tataaatatc gctttttgaa caaaaactgt ctaaaattga cctaaatgtt
    33781 aaataacaaa agtaactttc agttacttgg aaattaaaaa aagaacttgg cgaaacaacg
    33841 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
    33901 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
    33961 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
    34021 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
    34081 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
    34141 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
    34201 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
    34261 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
    34321 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
    34381 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
    34441 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
    34501 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
    34561 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
    34621 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
    34681 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
    34741 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
    34801 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
    34861 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
    34921 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
    34981 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
    35041 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
    35101 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
    35161 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
    35221 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
    35281 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
    35341 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
    35401 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
    35461 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
    35521 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
    35581 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
    35641 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
    35701 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
    35761 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
    35821 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
    35881 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
    35941 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
    36001 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
    36061 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
    36121 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
    36181 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
    36241 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
    36301 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
    36361 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
    36421 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
    36481 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
    36541 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta
    36601 tttttaaata taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat
    36661 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
    36721 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
    36781 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
    36841 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
    36901 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
    36961 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
    37021 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
    37081 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
    37141 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
    37201 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
    37261 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
    37321 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
    37381 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
    37441 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
    37501 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
    37561 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
    37621 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
    37681 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
    37741 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
    37801 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
    37861 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
    37921 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
    37981 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
    38041 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
    38101 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
    38161 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
    38221 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
    38281 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
    38341 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
    38401 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
    38461 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
    38521 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
    38581 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
    38641 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
    38701 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
    38761 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
    38821 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
    38881 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
    38941 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
    39001 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
    39061 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
    39121 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
    39181 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
    39241 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
    39301 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
    39361 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
    39421 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
    39481 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
    39541 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
    39601 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
    39661 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
    39721 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
    39781 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
    39841 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
    39901 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
    39961 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
    40021 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
    40081 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
    40141 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
    40201 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
    40261 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
    40321 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
    40381 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
    40441 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
    40501 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
    40561 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
    40621 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
    40681 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
    40741 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
    40801 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
    40861 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
    40921 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
    40981 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
    41041 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
    41101 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
    41161 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
    41221 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
    41281 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
    41341 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
    41401 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
    41461 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
    41521 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
    41581 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
    41641 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
    41701 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
    41761 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
    41821 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
    41881 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
    41941 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
    42001 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
    42061 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
    42121 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
    42181 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
    42241 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
    42301 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
    42361 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
    42421 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
    42481 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
    42541 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
    42601 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
    42661 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
    42721 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
    42781 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
    42841 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
    42901 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
    42961 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
    43021 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
    43081 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
    43141 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
    43201 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
    43261 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
    43321 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
    43381 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
    43441 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
    43501 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
    43561 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
    43621 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
    43681 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
    43741 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
    43801 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
    43861 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
    43921 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
    43981 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
    44041 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
    44101 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
    44161 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
    44221 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
    44281 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
    44341 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
    44401 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
    44461 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
    44521 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
    44581 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
    44641 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
    44701 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
    44761 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
    44821 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
    44881 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
    44941 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
    45001 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
    45061 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
    45121 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
    45181 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
    45241 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
    45301 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
    45361 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
    45421 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
    45481 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
    45541 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
    45601 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
    45661 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
    45721 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
    45781 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
    45841 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
    45901 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
    45961 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
    46021 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
    46081 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
    46141 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatagttt
    46201 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
    46261 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
    46321 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
    46381 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
    46441 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
    46501 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
    46561 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
    46621 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    46681 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    46741 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    46801 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    46861 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    46921 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    46981 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    47041 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    47101 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    47161 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    47221 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    47281 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    47341 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    47401 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    47461 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    47521 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    47581 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    47641 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    47701 aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    47761 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    47821 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    47881 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    47941 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    48001 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    48061 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    48121 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    48181 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    48241 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    48301 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    48361 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    48421 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    48481 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    48541 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    48601 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    48661 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    48721 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    48781 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    48841 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    48901 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    48961 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    49021 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    49081 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    49141 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    49201 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    49261 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    49321 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    49381 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    49441 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    49501 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    49561 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    49621 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    49681 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    49741 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    49801 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    49861 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    49921 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    49981 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    50041 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    50101 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    50161 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    50221 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    50281 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    50341 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    50401 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    50461 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    50521 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    50581 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    50641 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    50701 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    50761 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    50821 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    50881 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    50941 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    51001 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    51061 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    51121 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    51181 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    51241 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    51301 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    51361 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    51421 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    51481 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    51541 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    51601 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    51661 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    51721 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    51781 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    51841 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    51901 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    51961 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    52021 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    52081 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    52141 gcttgcatct gcggtaataa gcccaaccaa attaaaaaca taagaagctg tttttatttg
    52201 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    52261 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    52321 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    52381 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    52441 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    52501 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    52561 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    52621 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    52681 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    52741 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    52801 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    52861 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    52921 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    52981 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    53041 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    53101 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    53161 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    53221 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    53281 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    53341 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    53401 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    53461 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    53521 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    53581 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    53641 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    53701 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    53761 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    53821 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    53881 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    53941 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    54001 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    54061 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    54121 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    54181 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    54241 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    54301 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    54361 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    54421 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    54481 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    54541 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    54601 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    54661 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    54721 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    54781 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    54841 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    54901 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    54961 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    55021 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    55081 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    55141 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    55201 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    55261 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    55321 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    55381 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    55441 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    55501 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    55561 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    55621 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    55681 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    55741 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    55801 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    55861 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    55921 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    55981 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    56041 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    56101 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    56161 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    56221 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    56281 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    56341 ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt
    56401 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    56461 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    56521 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    56581 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    56641 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    56701 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    56761 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    56821 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
    56881 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
    56941 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
    57001 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
    57061 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
    57121 tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt
    57181 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
    57241 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
    57301 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
    57361 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
    57421 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
    57481 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
    57541 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
    57601 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
    57661 gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc
    57721 tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc
    57781 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
    57841 gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc
    57901 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
    57961 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
    58021 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
    58081 cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc
    58141 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
    58201 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
    58261 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
    58321 atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
    58381 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
    58441 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
    58501 agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt
    58561 cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
    58621 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
    58681 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
    58741 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
    58801 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
    58861 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
    58921 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
    58981 tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct
    59041 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
    59101 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
    59161 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
    59221 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt
    59281 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
    59341 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
    59401 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
    59461 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
    59521 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
    59581 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
    59641 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
    59701 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac
    59761 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
    59821 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
    59881 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
    59941 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
    60001 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
    60061 gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
    60121 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
    60181 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc
    60241 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
    60301 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
    60361 tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac
    60421 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
    60481 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
    60541 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
    60601 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
    60661 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
    60721 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
    60781 agatttttat ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct
    60841 gccttttagc ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt
    60901 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
    60961 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
    61021 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
    61081 taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc
    61141 catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt
    61201 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
    61261 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
    61321 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
    61381 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
    61441 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
    61501 tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
    61561 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
    61621 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt
    61681 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
    61741 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
    61801 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta
    61861 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt
    61921 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
    61981 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
    62041 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
    62101 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
    62161 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
    62221 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc
    62281 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
    62341 cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
    62401 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
    62461 tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
    62521 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga
    62581 atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct
    62641 tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg
    62701 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
    62761 gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc
    62821 atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
    62881 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
    62941 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
    63001 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
    63061 tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc
    63121 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
    63181 tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct
    63241 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
    63301 gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta
    63361 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
    63421 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga
    63481 acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
    63541 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa
    63601 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
    63661 aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc
    63721 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
    63781 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
    63841 tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg
    63901 ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc
    63961 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
    64021 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
    64081 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
    64141 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
    64201 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg
    64261 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
    64321 caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg
    64381 atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta
    64441 gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
    64501 cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
    64561 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
    64621 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
    64681 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
    64741 ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata
    64801 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
    64861 tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac
    64921 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
    64981 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
    65041 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
    65101 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
    65161 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
    65221 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
    65281 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    65341 agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    65401 ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca
    65461 cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc
    65521 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    65581 ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc
    65641 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    65701 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    65761 actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    65821 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    65881 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    65941 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    66001 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    66061 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    66121 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    66181 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    66241 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt
    66301 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    66361 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    66421 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    66481 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    66541 gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    66601 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    66661 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    66721 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    66781 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    66841 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    66901 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    66961 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    67021 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    67081 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    67141 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    67201 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    67261 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    67321 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    67381 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    67441 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat
    67501 aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca
    67561 aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg
    67621 tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa
    67681 tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat
    67741 ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt
    67801 ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt
    67861 tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg
    67921 tactttattt agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc
    67981 agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag
    68041 ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg
    68101 accaatgatc ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc
    68161 cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt tacattataa
    68221 atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca
    68281 cggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc
    68341 tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt
    68401 tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga
    68461 taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc
    68521 cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat
    68581 gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa
    68641 cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt
    68701 gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag
    68761 accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta
    68821 tattccccac atcttttttc atttattaaa actgcctcct t
//
LOCUS       DAZUKD010000002        59580 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000002 DAZUKD010000000
VERSION     DAZUKD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.002"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..421
                     /locus_tag="ACG8N9_000065"
     CDS             191..421
                     /locus_tag="ACG8N9_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4895706.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="ACG8N9_000066"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="ACG8N9_000066"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4895707.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="ACG8N9_000067"
     CDS             1832..2008
                     /locus_tag="ACG8N9_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4895708.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="ACG8N9_000068"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="ACG8N9_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4895709.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="ACG8N9_000069"
     CDS             2624..3091
                     /locus_tag="ACG8N9_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4895710.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="ACG8N9_000070"
     CDS             3176..4060
                     /locus_tag="ACG8N9_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4895711.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="ACG8N9_000071"
     CDS             4174..4410
                     /locus_tag="ACG8N9_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4895712.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="ACG8N9_000072"
     tRNA            4498..4578
                     /locus_tag="ACG8N9_000072"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="ACG8N9_000073"
     tRNA            4585..4656
                     /locus_tag="ACG8N9_000073"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="ACG8N9_000074"
     CDS             4781..4951
                     /locus_tag="ACG8N9_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895713.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="ACG8N9_000075"
     CDS             5171..5878
                     /locus_tag="ACG8N9_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4895714.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="ACG8N9_000076"
     CDS             complement(5937..6581)
                     /locus_tag="ACG8N9_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4895715.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="ACG8N9_000077"
     CDS             6707..6994
                     /locus_tag="ACG8N9_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895716.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="ACG8N9_000078"
     CDS             7102..7422
                     /locus_tag="ACG8N9_000078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4895717.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8817
                     /locus_tag="ACG8N9_000079"
                     /pseudo
     CDS             7503..8817
                     /locus_tag="ACG8N9_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            8814..9533
                     /locus_tag="ACG8N9_000080"
     CDS             8814..9533
                     /locus_tag="ACG8N9_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS4895718.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9661..10212
                     /gene="raiA"
                     /locus_tag="ACG8N9_000081"
     CDS             9661..10212
                     /gene="raiA"
                     /locus_tag="ACG8N9_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4895719.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10434..12968
                     /gene="secA"
                     /locus_tag="ACG8N9_000082"
     CDS             10434..12968
                     /gene="secA"
                     /locus_tag="ACG8N9_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4895720.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13094..14195
                     /gene="prfB"
                     /locus_tag="ACG8N9_000083"
     CDS             join(13094..13165,13167..14195)
                     /gene="prfB"
                     /locus_tag="ACG8N9_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4895721.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14300..14986
                     /gene="ftsE"
                     /locus_tag="ACG8N9_000084"
     CDS             14300..14986
                     /gene="ftsE"
                     /locus_tag="ACG8N9_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4895722.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14979..15863
                     /gene="ftsX"
                     /locus_tag="ACG8N9_000085"
     CDS             14979..15863
                     /gene="ftsX"
                     /locus_tag="ACG8N9_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4895723.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16015..17463
                     /gene="cls"
                     /locus_tag="ACG8N9_000086"
     CDS             16015..17463
                     /gene="cls"
                     /locus_tag="ACG8N9_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4895724.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17800..18657
                     /locus_tag="ACG8N9_000087"
     CDS             17800..18657
                     /locus_tag="ACG8N9_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4895725.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18772..19692
                     /gene="pstC"
                     /locus_tag="ACG8N9_000088"
     CDS             18772..19692
                     /gene="pstC"
                     /locus_tag="ACG8N9_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4895726.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19692..20576
                     /gene="pstA"
                     /locus_tag="ACG8N9_000089"
     CDS             19692..20576
                     /gene="pstA"
                     /locus_tag="ACG8N9_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4895727.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20591..21397
                     /gene="pstB"
                     /locus_tag="ACG8N9_000090"
     CDS             20591..21397
                     /gene="pstB"
                     /locus_tag="ACG8N9_000090"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4895728.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21413..22171
                     /gene="pstB"
                     /locus_tag="ACG8N9_000091"
     CDS             21413..22171
                     /gene="pstB"
                     /locus_tag="ACG8N9_000091"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4895729.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22184..22861
                     /gene="phoU"
                     /locus_tag="ACG8N9_000092"
     CDS             22184..22861
                     /gene="phoU"
                     /locus_tag="ACG8N9_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4895730.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23438..24130
                     /locus_tag="ACG8N9_000093"
     CDS             23438..24130
                     /locus_tag="ACG8N9_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4895731.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24127..24894
                     /locus_tag="ACG8N9_000094"
     CDS             24127..24894
                     /locus_tag="ACG8N9_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4895732.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24994..26562
                     /gene="liaX"
                     /locus_tag="ACG8N9_000095"
     CDS             24994..26562
                     /gene="liaX"
                     /locus_tag="ACG8N9_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4895733.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26859..27179
                     /locus_tag="ACG8N9_000096"
     CDS             26859..27179
                     /locus_tag="ACG8N9_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4895734.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27179..27529
                     /locus_tag="ACG8N9_000097"
     CDS             27179..27529
                     /locus_tag="ACG8N9_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4895735.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27696..28634
                     /gene="hprK"
                     /locus_tag="ACG8N9_000098"
     CDS             27696..28634
                     /gene="hprK"
                     /locus_tag="ACG8N9_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4895736.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28649..29479
                     /gene="lgt"
                     /locus_tag="ACG8N9_000099"
     CDS             28649..29479
                     /gene="lgt"
                     /locus_tag="ACG8N9_000099"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4895737.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29523..30548
                     /locus_tag="ACG8N9_000100"
     CDS             29523..30548
                     /locus_tag="ACG8N9_000100"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4895738.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30563..31501
                     /gene="galU"
                     /locus_tag="ACG8N9_000101"
     CDS             30563..31501
                     /gene="galU"
                     /locus_tag="ACG8N9_000101"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4895739.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31626..32066
                     /locus_tag="ACG8N9_000102"
     CDS             31626..32066
                     /locus_tag="ACG8N9_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4895740.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32069..32671
                     /locus_tag="ACG8N9_000103"
     CDS             32069..32671
                     /locus_tag="ACG8N9_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4895741.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32730..33836)
                     /locus_tag="ACG8N9_000104"
     CDS             complement(32730..33836)
                     /locus_tag="ACG8N9_000104"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4895742.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34046..35047
                     /gene="ccpA"
                     /locus_tag="ACG8N9_000105"
     CDS             34046..35047
                     /gene="ccpA"
                     /locus_tag="ACG8N9_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4895743.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35384..37729)
                     /locus_tag="ACG8N9_000106"
     CDS             complement(35384..37729)
                     /locus_tag="ACG8N9_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4895744.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     RIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38008..38910
                     /locus_tag="ACG8N9_000107"
     CDS             38008..38910
                     /locus_tag="ACG8N9_000107"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4895745.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39123..40898
                     /locus_tag="ACG8N9_000108"
     CDS             39123..40898
                     /locus_tag="ACG8N9_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4895746.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40964..42550)
                     /locus_tag="ACG8N9_000109"
     CDS             complement(40964..42550)
                     /locus_tag="ACG8N9_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4895747.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42801..43025
                     /locus_tag="ACG8N9_000110"
     CDS             42801..43025
                     /locus_tag="ACG8N9_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4895748.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43167..44918
                     /locus_tag="ACG8N9_000111"
     CDS             43167..44918
                     /locus_tag="ACG8N9_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4895749.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44918..46702
                     /locus_tag="ACG8N9_000112"
     CDS             44918..46702
                     /locus_tag="ACG8N9_000112"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4895750.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47028..47879
                     /gene="licT"
                     /locus_tag="ACG8N9_000113"
     CDS             47028..47879
                     /gene="licT"
                     /locus_tag="ACG8N9_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4895751.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47972..49858
                     /locus_tag="ACG8N9_000114"
                     /pseudo
     CDS             47972..49858
                     /locus_tag="ACG8N9_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(49898..50800)
                     /locus_tag="ACG8N9_000115"
     CDS             complement(49898..50800)
                     /locus_tag="ACG8N9_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4895752.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51031..52434
                     /locus_tag="ACG8N9_000116"
     CDS             51031..52434
                     /locus_tag="ACG8N9_000116"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4895753.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52434..53867
                     /locus_tag="ACG8N9_000117"
     CDS             52434..53867
                     /locus_tag="ACG8N9_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4895754.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54012..54938
                     /gene="trxB"
                     /locus_tag="ACG8N9_000118"
     CDS             54012..54938
                     /gene="trxB"
                     /locus_tag="ACG8N9_000118"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4895755.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55152..56879
                     /locus_tag="ACG8N9_000119"
     CDS             55152..56879
                     /locus_tag="ACG8N9_000119"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4895756.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56894..57244
                     /locus_tag="ACG8N9_000120"
     CDS             56894..57244
                     /locus_tag="ACG8N9_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4895757.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57336..58196)
                     /locus_tag="ACG8N9_000121"
     CDS             complement(57336..58196)
                     /locus_tag="ACG8N9_000121"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4895758.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58288..58716
                     /locus_tag="ACG8N9_000122"
     CDS             58288..58716
                     /locus_tag="ACG8N9_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4895759.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58879..59055
                     /gene="rpsU"
                     /locus_tag="ACG8N9_000123"
     CDS             58879..59055
                     /gene="rpsU"
                     /locus_tag="ACG8N9_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4895760.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59082..59528
                     /locus_tag="ACG8N9_000124"
     CDS             59082..59528
                     /locus_tag="ACG8N9_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4895761.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19398 a        10338 c        12771 g        17073 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt ttttttggac aggacagctt
     7801 actgaaaaac aaacggaaat ctcaaagaga atactttgcc attcagaaaa aagacatcat
     7861 cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat tttagtggaa
     7921 actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt ttgtaatgaa
     7981 ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct ccatgggaat
     8041 acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca gctataccgt
     8101 ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc ttataacggg
     8161 gatgcaggat tagctcatgc tgtgcaaaca gcgattaaac cagacggacg attcatctac
     8221 ttaagtgcca cacctgataa aaagttacta aaggagatca agcagacgtt tgacatccac
     8281 aaacttgctt tacgctttca tcggcgtttg cttcctgaac ccgtcttggt tttcatgaat
     8341 aattggcgac aaaaatgcaa gaacaagaag aagataaaat ctttggtgcg gatcattcat
     8401 caattaatta aagaaaacca tatcctttta ttttgtccaa gtatcacgat gatgcatcgt
     8461 ttgaaagcca cacttcaaga ggctcttccg agatatgaga tcacagatgt ttcttcaaga
     8521 gacaatcagc gggcggaaaa agtccagaaa atgcgagaag caaaatatga catactgtta
     8581 accacgatga tccttgaacg tggagtcaca tttgaaagaa tatccgttat cgttttagga
     8641 gcggatcatc cggtttttac taaatcttct ttggtacaaa tcgcaggaag agccgatcgt
     8701 aaagggcctt atacgaacag tcaagtatat tttttctatg aagaaaaaac tgcagctatt
     8761 cgaaaagctt gtcaggaaat caaagaaatg aatgaagaag ggaagaagat actgtgaggt
     8821 gcagttgctg cagtaaagat atcgtccaaa atttaacatt cagcgagatt attggtgttt
     8881 cgaaatacag attacctcag acttgttcag tttgtcgcgc acaatttcat ttattggaaa
     8941 caagaggctg ttctggctgt caaaaaccaa tccatttcaa tctagagagt acgaaaaaga
     9001 aagtaaattc tatccccgtt tactgcagtg actgtcaaga atggaagaag aaatatccga
     9061 cctatgattt tcagcaccgt gccttttttg tgtacgatga aaatatgaag gcctggctca
     9121 atcaatacaa gtttatggga gatatccgac tagcaggcac ctttgcatat aaatggacgc
     9181 agataaaaaa agaatacccg acatacatct attgtccaat acctttatct gaaaaaaggc
     9241 gaacagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaga attccttttc
     9301 agatgctact caaaagagag aagcatctgc tggctcaagc aaaaaacacg cgaaaggaaa
     9361 gattggagat gcctcaacct tttgaacttg cagtcacctt ttctaagata caaaataaaa
     9421 aaatattgat tattgatgat gtctatacaa caggacagac tcttttccac gcagcagatt
     9481 gtttgcagat cgcacagccg aaaatcatcc gaactatttc tttggcgaga tgaattagat
     9541 gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata atagaattaa
     9601 gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg ggtaattttt
     9661 atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat tcgcgactac
     9721 gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga tgcaacggcg
     9781 catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac gatcccatta
     9841 ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag tatcgatttg
     9901 gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa tcgtaaatcc
     9961 cgtgaaacgg gcatgaatac tgcaaatgct gctgctttat tcaatgaaga ggacgaaaac
    10021 gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa accaatggat
    10081 agtgaagaag cagtcttgca aatgaacatg ctgggacata acttctttat ttttgaagat
    10141 gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata cggactgatt
    10201 gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc tactgacagc
    10261 tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt
    10321 tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt
    10381 aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca
    10441 atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg
    10501 caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg
    10561 caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc
    10621 cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta tatccatacc
    10681 acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa atgcgtactg
    10741 gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg actggcgaag
    10801 gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg
    10861 aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa tcttcggatg
    10921 aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa ttaggatttg
    10981 actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt ccgctgaatt
    11041 atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg ccgctgatca
    11101 tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac ttcgtgaaac
    11161 gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc agtttaaccg
    11221 aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac gacattgaaa
    11281 atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc atgcttttag
    11341 atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc actggtcgga
    11401 tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct aaagaaggcg
    11461 tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac tatttccgta
    11521 tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa gaattcagag
    11581 aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc cgtgatgatc
    11641 gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg caagatatca
    11701 aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt gaaacatctg
    11761 agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg aatgcgaaaa
    11821 atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct gtgacgattg
    11881 ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta agagaacttg
    11941 gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc
    12001 gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg tcattggaag
    12061 atgatttgat gaaacgattc ggttcagaac gaatcaaatc tttcttagat cggatgaaga
    12121 tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta gaatctgcac
    12181 aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa tacgatgatg
    12241 tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc atggaagaaa
    12301 aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt gtagtagatg
    12361 cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat tttgccggca
    12421 gtactcttgt ccatgaagat agtattactc ttgcagatat cgaaggaaaa acagctcagg
    12481 agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa gcggctcaac
    12541 taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt gttgtggact
    12601 ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc ggcttacgtg
    12661 cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag atgtttgaag
    12721 atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct gagatccgtc
    12781 aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa gacggagatg
    12841 ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaac aaggtcggaa
    12901 gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat ggaaaaaatg
    12961 caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga attcgtctca
    13021 ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa gtatagtgaa
    13081 agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa tgcaggaaaa
    13141 ggtcacaagc ttcaggaggt ctctttgact tagaccagct ggaagaagac attgcggaag
    13201 cagaagcaaa aatggctgaa cctggttttt gggatgatag cgaagctgct caaaaagtga
    13261 tcagtgaaaa caatgcaaat aaagaaactt atgatcaatt ccattctctt gctgaagaac
    13321 tggaagaaat cgaattgatg gtggagatgt tgcaggaaga acctgatgca gccatgcaag
    13381 aagaagcaga aacacgtatc aatgcgctag atgaaaaaat gaagacatat gaattatcta
    13441 tgttgttaga tggtccttat gatcgaaaca acgccatcgt tgaattgcat ccaggggctg
    13501 gcggaacgga gtcccaagat tggggcagta tgcttttacg tatgtataca cgctgggcag
    13561 agcagcatgg gtatcaagta gagacactag attatcaagc aggtgatgaa gcaggaatca
    13621 agagtgtgac cctactgatc aaaggacaca acgcatttgg ttatctgaaa tcagaaaaag
    13681 gtgttcatcg ccttgtacgt atctcgcctt ttgactcagc gaaacgacgt catacttctt
    13741 tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa atcaatccag
    13801 atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac atcaataaaa
    13861 cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct agtcaggcac
    13921 aacgttcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct aagctttatc
    13981 aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa caaaaagaaa
    14041 tcggctgggg ttcacagatc cgttcttatg ttttccatcc atattctatg gtaaaagacc
    14101 atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac cttgatttat
    14161 ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa ttgaaataaa
    14221 attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta aagactgaaa
    14281 aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt actcaaatgg
    14341 tacgaccgct atccgcaatc tctcaattgt catcgaacaa ggagagtttg tctatgtggt
    14401 tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag aagaaaaagc
    14461 ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa accgcgaagt
    14521 tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat taccaagaaa
    14581 aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac ctcgggatat
    14641 caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag ttcgagtctt
    14701 tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg caatagtgaa
    14761 tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag aaaactcatg
    14821 ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg tgatggctac
    14881 acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg aaaacggccg
    14941 cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc tttttccgcc
    15001 atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt gcttcagtca
    15061 gtgcggtaac gatcacattg actttagtcg gtgtattcat ggcagttatc atgaacgcaa
    15121 ctaaattagc tcaagatata gaaggaaacg ttgatgtctc tgtattcgta gatatcggta
    15181 caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat catgttaaaa
    15241 aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa atgggtgatt
    15301 cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta gtaagtgcta
    15361 ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag aatgtttctc
    15421 gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt gcagttcgga
    15481 catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc atttcgaaca
    15541 cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt ttagtcggtg
    15601 caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc ggtctgatcg
    15661 gtgcgattct tccgattttg atcatcacat ttggttatgc atggtttttc caattgatca
    15721 atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc gtttggaaaa
    15781 tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg atcatttcta
    15841 tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag ttggacagtt
    15901 tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct tttttccctt
    15961 tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga agccgtgaaa
    16021 attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct ttcctttatc
    16081 atcgtttttc gagaaagaaa ggaaactgct caaacatggg catgggtgct cgttttgatg
    16141 ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat ttccaaagag
    16201 aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat tgaagaagac
    16261 aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt agatgtgaaa
    16321 caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa aaatgaaatc
    16381 actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat caagcaggca
    16441 gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg tgaagaagtt
    16501 cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct tcttgatgca
    16561 tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc aggcggagaa
    16621 gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa ttatcgcaat
    16681 catcgtaaaa tcgtagttat tgatgggaaa atcggatata caggtgggtt caatgttgga
    16741 aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca tttgcgtatt
    16801 tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa ttctcagcat
    16861 acaaaagaag taggctattc tcccaaattt tttccgtcca ttcactctac tggtgatata
    16921 gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa aatgacttat
    16981 ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta ttacataccg
    17041 gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa agttcatatc
    17101 caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc tttcgctgca
    17161 gagctaatcg agtatggagc aaggatcgaa acatatgaga atggatttat tcatgcaaag
    17221 acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga tgttcgttca
    17281 tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc ttcacgtgta
    17341 agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga aatgtatgaa
    17401 aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc gccgctcctt
    17461 taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc cttttctaac
    17521 ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag cgaaattaac
    17581 tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat gctattaaaa
    17641 gagacacatt tcaaagccag tatagcatag aaacttttaa tgagatagtt ttttttacaa
    17701 ttatgagaat ttattttgtt atttaatgat tttgattcgt tttcatcaag taatgaaaat
    17761 gataaaataa acatacttta ggattaaagg agatttctaa tgaaaaaact taaattattc
    17821 ttaccaataa taggattagc tattctttta tcaggctgtt caaagtggat tgatcgagga
    17881 gaatccatta cggctgttgg ctcttctgct ttacagcctt tagtagaaac agttgctgaa
    17941 acttaccaga cagaaaatcc tggaaaattc atcaatgtac aagggggcgg aagcggaact
    18001 ggtttgtctc aagtacagtc aggagcagtc gacatcggaa actctgattt gtttgcggaa
    18061 gaaaaatcgg ggatcgatgc ctcagcatta gtcgatcatc gagtagcagt tgttgggatc
    18121 actcctattg tgaacaagga tattggcgta aaagacatca gtatggaaaa cctgaaaaaa
    18181 ctatttacag gcgagatcaa aaactggagt gaagtcggtg gaaaagatca gccagtagtt
    18241 atattgaacc gcgcttcagg aagcggaacc cgtagcacgt ttgaaaaatg ggtattggac
    18301 ggagaaacag ctgttcaggc acaagaacaa gattcaagcg gaatggtgcg ccagatcgtt
    18361 gccgacacac ccggtgcaat cagctatgtt gctttttctt atgtttccga tgatgtagat
    18421 acgttgaatg ttaacggtgt agaaccgaca gatgaaaatg tgacaacgaa caaatggacg
    18481 atttgggcat atgagcatat gtacacaaga gatcatccaa aaaaactgac aaaagaattt
    18541 ttagactata tcttatctga tgaagtacaa gataacatcg ttggagaact aggttatatc
    18601 cctgtttctc atatgaaagt cgaacgtgat tggcaaggaa atatcgttaa agagtaatct
    18661 ttacacaaaa tttacaaact tagctaaact tttacatgtt attaaaacca aattgatgtt
    18721 caatttgtac actgagtgag tatgttaaac ctgattaagg gggagtcatc tgtggaagat
    18781 gtgaaaaaag tattattaac aaaatcaaaa cgttcaaaaa tggaacagcg tggaaaattc
    18841 atcagttttt tatgtatcgc gctgatcgtg ttggttgtat tatctatttt ttattttgta
    18901 gctagtaaag gattggccac cttttttact gataagatca atgtatttga ttttctgttt
    18961 ggtacagatt ggaatccaag ccaaatcgga gcaaatggga aaccaatggt aggggcattg
    19021 cctatgatca caggttcgtt tattgtgacc tttttatctg ctatcgttgc cacgccattt
    19081 gccattggtg cagcagtatt catgacggag atctctccta aaaacggaac gaagatctta
    19141 cagccggtca tcgagctgct tgtaggtatt ccttctgtcg tttatggttt tatcggttta
    19201 tcagttatcg tgccttttgt cagaagtatt tttggcggga ctgggtttgg tatcttagct
    19261 ggtacatttg tgttgttcgt tatgatcttg ccgactgtca caacgatgac tgttgatgca
    19321 ttaaaggcag tgccacggca ctatcgggaa gcttcattag cgcttggtgc cacaagatgg
    19381 caaacaatct acaaagtcgt gctacgagca gccgtgcccg ggattttaac agcagtcgtt
    19441 tttggtatgg ctcgtgcgtt tggtgaagcc ttagctatcc aaatggtcat cggtaatgca
    19501 gcattgatgc caacaggtct tactactcct gcttctacat tgacgtccat cttaacaatg
    19561 ggaatcggaa atacaatcat gggaacagtt gaaaataatg tgttatggtc attagcgctg
    19621 atcttgctat tgatgtcatt attctttaat atcgtgatcc gtatgattgg gaagaaagga
    19681 gagctgaaat aatgaacgca aaacgctcag ataaaattgc tacaggtgtt ttatatatag
    19741 tttcggggat catcgtattg attttagcag ctttactact ttatatctta gttcggggaa
    19801 taccgcacat ttcatggtcc ttcttgaccg aaccttccaa agcctaccaa gaaggcggtg
    19861 gaatcgggat ccagctattt aactcgattt atttgctttt gatcacgatg atcatcagtt
    19921 ttcctatttc tttaggtgcg ggtatctatc tttcagaata tgctggaaaa aactggctaa
    19981 cagatgtgat tcgcacgtcg attgaaatct tgagctcgtt gccttctgtc gttgtcggtt
    20041 tattcggttt cttggttttc gttattcaat tccagtatgg tttctcgatt atttctggtg
    20101 cattggcttt gactgtattc aacttgcctc ttttgactag aacagttgag gagtctttac
    20161 aagcggtcca ttacacacaa cgagaagcag gattggcctt aggtttgtca cgctgggaaa
    20221 cagtgataaa agtggtggtt ccagaagcga caccagggat tttgactggt gtgatcctaa
    20281 gttctggaag aatttttggt gaagcagctg cattgatcta tacggctgga caaagtgcac
    20341 ctgcgttaga ttttacgaac tggaatccat taagtgtttc aagcccgatc agcattttcc
    20401 gtcaagctga aacattagct gtccatattt ggaagatcaa tacagaaggc acgatgcctg
    20461 atggtgtttc tgtttcggct ggcgcttctg ccgtgttgat tattgctgtt ttactgttca
    20521 acttcggcgc tcgtgcgatt ggcaaacgat tataccgaaa aatgacttca gcctaaggag
    20581 aagaagcaaa atgaaaaaat ataactggaa tgaaacaaat gtcatcacca tgccaaaaga
    20641 gaaagaagta gcactatata cggatgatct acatgtctgg tatggcgaca atgaagcaat
    20701 caaaggagtg gatctggaat ttgaaaaaaa taagatcact gcactgattg gtccttccgg
    20761 ctgcggaaaa tctacgtatc ttcgttcgtt gaatcgaatg aatgatggga tcgctaatac
    20821 gaaaatcact gggaaaatca tgtatcaagg cgtagatatt aatgcgcctc aagtggatgt
    20881 ttatgagatg cgcaaacgga ttggcatggt ttttcaacgt cctaatccgt tcagcaagtc
    20941 gatttatgac aatattacat ttgctttgaa acagcacggc gaaaaaaaca aaaagaaatt
    21001 agatgaaatc gttgaaacaa gcttgaaaca agctgccttg tgggaccaag taaaagacag
    21061 cttggacaaa agtgccttgg ctttatctgg cgggcaacag caacgtttat gtattgctcg
    21121 tgcgatcgcg atgaaacctg atatcttgct gctagacgag ccggcaagtg ctttggaccc
    21181 tatttcgaca ggaacggttg aagaaacact tgtaaacttg aaagaagact atacgatcat
    21241 catcgtgacg cataacatgc aacaagcagc tcgtatcagc gactatactg cctttttcta
    21301 tttaggaaaa gtaatggaat atgacaaaac taacaagata tttaccagac caaaaattca
    21361 ggcaaccgaa gattatgtgt ccggtcattt tggttaagac ggaggcctca acatgacaaa
    21421 agagattatt tcatctaaag atcttcatct gtattatgga aagaaagaag cactaaaagg
    21481 gatcgatcta agcttcaatc aaggggaaat cactgcgatg atcggtcctt ctggttgcgg
    21541 aaaatccact tatctgcgtt ctttgaaccg gatgaatgac ctgattccga atgtaacgat
    21601 caccggaagt gttttatata aagacaaaga catctatggt ccaaaaacag atgttgttga
    21661 actaagaaaa gaaatcggca tggtattcca gcagccaaac ccatttcctt tttctatcta
    21721 tgaaaatgtc atctatggat tgaaattaaa aggggaaaaa gataaaaaag tccttgatca
    21781 agtagttgaa gaaagcctga aggcagcgtc tgtttgggat gatgtgaaag acaaattgca
    21841 taaaagtgct ttatctttat ccggtgggca gcagcagcgt gtatgtatcg cacgtgtttt
    21901 ggcagttaat ccggaaatca tcttattgga tgaaccaaca agtgcgcttg atcctgtttc
    21961 tactgggaaa atcgaaagta tgctgttaga attaagagag cggtatacga tgatcatcgt
    22021 gacccataat atgtctcaag cttcacgtat ttcagataag acggcattct ttttagatgg
    22081 tcacttgatc gaatacaatg atacgaagaa gatattcatg aatccagaaa aacaagagac
    22141 agaagattat atttcaggtc gatttggata aggaggaaac ggaatgctac gtacacaatt
    22201 tgaagaagaa ttgctgaatc tccataatca gttctacgaa atggggatga tggtgagcag
    22261 cgccgtgcac aagtcagttc gtgcatacat caaacacgat aaaatcttag cccaagaagt
    22321 tatcgaaaac gatataaata tcaataacat ggaaacacgc ttggagaaga aaagttttga
    22381 aatgatcgca ttgcaacagc cagttacaac tgatttgcgt atgatcatta cggtcatgaa
    22441 agctagctcg gatttagaac gtatggccga tcatgcggtg tctgttgcta aatccacgat
    22501 tcgcgtaaaa gggcagacga gaataccgga aatcgaaaaa gagatctccg atatgtccga
    22561 ttatgtgaaa aaaatggtgg acaacgtcct agttgcttat gtcaaaacag acgaaaaaga
    22621 cgcacgtacg atcgccaata tggatcaaag agtcaatgac tatttcaatc gaatctataa
    22681 tgcaacaatc aaaaatatgc aggaaaatcc tgagacagtc atcagtggaa cagactactt
    22741 gaatgttgcc agttatctag aacggatcgg agactatgtg acaaacatct gtgaatggat
    22801 cgtctacttg gctacaggga aaatcactga gttaaatacg aaccacaatg agattttcta
    22861 aactgaagct gtgattatgc cgtttgttta ggaaaagagg ttgtgaaatc agcggttgtg
    22921 aaaaagctgt tctgcatcaa gtaataagaa cagtcaaaca aaaaatagct cctcatattt
    22981 ttcgtttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgggaaa
    23041 gggtttgtga caaaacatgt cacaaaccct tttcatcatt tcatagaaag gataaagaat
    23101 gcaaaaaaaa attagcatca tcactatatt ttttatttgt gtgcttactt catgggttat
    23161 tcagcatcaa gccgctttat atgaagtacc tgtcatcgag atagaaaaag tacgaataaa
    23221 agaggatcat caattgatta caggaaaact gatgaacacg gatcaaaaag gagaatcgat
    23281 ttatttatcg gttccttatc aaaaaaatca aatagaaaca gcaaatataa aagcgagaca
    23341 aatgtatcta gtccaaatgc atggagatga atggcagcta gtagcaaaaa aaaatgatgg
    23401 ctggcttttc ttcttcacaa gtatcttcat tggcgccatg ttgcttattg gtggaaaaca
    23461 gggtctattt gctttaggaa gtctcatcat caatatcgtt ttacttttac ttttgttgct
    23521 catctttact aaaacagaag ggatttctct attaaatata tctatcctgt tcgtcgttat
    23581 agggatgatt attagcatat tggctttaga tggatggaat agaagcagta taatcaaaat
    23641 ttcagctgca gttacgagtg tttttttagc attttttatt tgtcttttga caatgaatca
    23701 ttggaaagac caaggactgc gctatgagga actgaattat ctgacacgac cgtatcgttc
    23761 tgtttttctt tctagcgtac tgattggaac agctggagga acactcgata ccgttatcac
    23821 agtcattgcg acattagaag aattgactaa gcgacaacca gaaataacag cgaaacagtt
    23881 atggctttca ggaaggcaag tgggtcgaga tgtgattggc tcgatgacca atatcctttt
    23941 gttcgtctat atcagtgggt cagttccatc gcttctttta tatttaggaa atcaatggtc
    24001 gttcaaagaa acgatagaac agcatttatc attggaattt ttacgcgtca ttgctggcag
    24061 cttaggaata gtattatcga tccctatctc ggtcctcttt tttctgactg taaggaggct
    24121 gaaacgatga atgtacttat tatacttgtt gggattttag caatcagcgt gctacttatt
    24181 ggcggtacgc aaggtatgta tattctttta ggcttattca tcaatttagg gatctttttc
    24241 ctgttgttat tcggttatca tcagaagtgg ccgatactgg cattgtcggt cataggattt
    24301 ttattgattg ctgtggttat cttatttttt atcaacggtt ataatctaaa aatgcgtgca
    24361 gcttttgtga gtatactgat ctttttattt tgtttcctac tattgcttcc tatcactgat
    24421 tttttagcaa tccaaggttt tacatcggta gaactagaag aattatctgg cttagataag
    24481 actttagcga ttgattttag attacttacg agatcgttgc ttctgattag tcttagtggt
    24541 gctgttttag atgcgagtgt agcaatttct agtgggacat tcgaagtata tcaggcaaat
    24601 ccgcaattat ctttttacca attgcgccat tctagtttcg ctattgctaa aaaaatattg
    24661 gcgtcaacag taatgacact tctttttgca ttcatgggaa gttctttagc gttgatcctc
    24721 tggtttattg atttagacat tccttttcag caagtgatca atgaaaaatc atttgttcta
    24781 gaatatacga tggctattct taccacattg tccgcactac ttgttcttcc tttaacctgc
    24841 gtgactacag catatctctt tacaaaaaag aaagaacaac tcaagatgga gtaaaagtta
    24901 caacttaagt cctatgacat ttttactgaa tcagtgcata atatggatgt aaggaaaagc
    24961 ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag aaagagaacg tattttagat
    25021 ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag atttattaga aagcatggca
    25081 actgaaaaag acgaaaagca aatcaaaaaa gaagcagaca aggtaactgc tagtcataaa
    25141 gaaaaagatc aggcaagtca attgatcgat aaacttgaaa acggcgaaga agaaatttct
    25201 gagccagttg atccaaagga aagagaaaga caagatcaag aaaacttaga aaagatctta
    25261 gatgaactag caactgaagc gaataaaact tcggcgcgtc tagatgaagt aaatgctcaa
    25321 ttcgctgaca ataaggcagc acgcaatgag aaacaggaag cattgatgca gctgaataca
    25381 aaagaagaat taggcgagtt gacagaagaa gaattagcac agcgtcagac gttagaagca
    25441 gaaatcaaag aattagaagc tagtggcgat acactgttag aagaacaatt gaaattagaa
    25501 gcagaattaa aagatatccg taagaatcaa tggtctgaga aaaaagaaac gtttactgat
    25561 aagtttgaat tgccagatga ttggaaagat caagcaacag atacactgaa tcaagtaggc
    25621 gaaaaaatgt cagaagctgg atcacagtta ggcaaattct tgaagaaaac attccaaact
    25681 gtttcagaaa cagtgaatga caacatggaa tggaaagatg tcagcttacg tgtccctggg
    25741 attgcgacaa cgaaatttga gcatgaattt tactatgaag ctcctgcagc aagtatcctt
    25801 gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt gggacagcaa cgatgtgaaa
    25861 gttgaagcta agatcaaact ttacggaaaa atgggtgctg aaccatttga agcattctct
    25921 gaacggagcc agattgaggt taatgaagat catatctcct tccaaattcc aaataaacgt
    25981 gtgcgtgcag accttgtttt ctacttacct aaacgagtat acgaccacgc agctattaaa
    26041 cttctaaatg gcaacatcat gattgaaaca ttggaagcta aagatattta tacaaaatct
    26101 acgaatggta acatcatcgt ggaccaatta actgcaacga tgctggaagt agaaggtgta
    26161 aacggcaata tcgatattcg aaacgggaac atcttagatt ctattatcga aacagtaaat
    26221 ggtacagtga catttggtgc gacagcagaa aacctttctg tgtctttggt aaatggtgat
    26281 gtacgtctta cgattaaaga agataatttg aaaaaagtag aagcaagttc agtgaacgga
    26341 aatgtcaaag tcgctttacc agatggcatc ggtttggaag gacatgctaa aacaagtctt
    26401 ggaagtatca acagccgtct gtccaattac gaagtagttc gtgagaaaaa agaacggaca
    26461 aaccaaatgt tgcaattccg tcgtgtttca gatggtgaaa tcgcgcaagt gcaactttca
    26521 acgacaacag gaagtatcta tttaaaagat acagataaat aaagaggttg tgaaagagat
    26581 gttcagcctt gagcaataag aagcgatttt gtaaaatagc ttcccctatt ttacaaaatt
    26641 gtgccttatt gccgatagtc tatctcttga acaccgttta ccaagagatt atgaacctgc
    26701 caattatcgc cgagtcacaa ggaataattt cagaaaaagc tcgtcacgtt ttatgaatat
    26761 ggtaatttgt gaccgagaat tttgcagaaa gacaccgatt aagaagaagg ttgtcacaac
    26821 gattttcttc atttaaaata gtagaacaga ggtgagacat gatgaaaaaa aagttgacaa
    26881 aatctaataa aaatgttgtt ttgacaggga ctttagcagg aataggagaa tacatcggga
    26941 ttgatccgac tgttatccgt gtgctttatg tattcctgag cttagttgct tttggaagtc
    27001 caattatttt atacatttta ttagctttga ttatcccatc tgcgcgttct gcacgaacaa
    27061 actatggaca taacaatgca tattatgaaa aaaacaatta tcgtgaacaa actcgtgaac
    27121 aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga cgaatggagt gacttttaat
    27181 gtcgtacttt caacgtattt tagttaatac gttaacattc atatcattgg ctgttctttt
    27241 tcctgcgatg gtccatgtga gcagtatttg gacggccgtt cttgccagct tcgtattgtc
    27301 tgtgttgaat atgtttgtca aaccgatttt gacgatactg tccctgccct ttacattagt
    27361 cacattggga ttgtttacgt ttgttatcaa tgcagcgatt cttaaaatga cctccttttt
    27421 tgtgggagca gaacatttta gtttttcttc attcggtgca gcgttgttag tagctatctt
    27481 gatgtcgctt gtcaatgtaa ttgtcagcga acataacagt agaaaataaa agaaagctgt
    27541 gccgaactat cggcgcagtt tttttattta gaatcaggaa tgcgttttct attacattgg
    27601 taattaaata aaaacgtttt ttttaattgg tagtactttt ggctagacaa tgataaaatg
    27661 atagagaaca ctctaataga aatgaggtag catgaatgtc agaagttgta aaagttagcc
    27721 aattagtgaa aaaattatct ttggaaatcg tgacaggcga cgagaaaagc ttagaccgtg
    27781 tcatcgtaac tggggacatc tcacgcccag gactagagtt gactggttat tttaattatt
    27841 attcccataa tcgtttacag ctatttggca gtaaagaaat cagttttgcg gaacggatga
    27901 tcccagaaga gcgtttgatc atcatgagaa gattatgtag tgaagatatg cccgcattta
    27961 ttatttcaag aggattacca gctccagaag aaatgatcca ggcagcaaat gaaaatggga
    28021 tagctgtact gcgttctccc atctcaactt ctcgtttatt aggagaatta tccagttatt
    28081 tagatggtcg ccttgcccct cgaacaagtg tccacggtgt tttagtggat gtttacggat
    28141 taggtgtact gattcaaggt gacagtggga taggaaaaag tgaaacagcg ttggagttga
    28201 tcaaacgcgg ccatcgattg atcgcagacg atcgagtaga tgtttatcag caagatgaat
    28261 taacggtgat cggagaaccg cctaaaatcc tggaacatct gattgaaatc cgaggaatcg
    28321 ggatcattga tgtaatgaat ctttttggtg cgagtgcggt acgtggctct atgcaagtcc
    28381 agttggctgt ttatctggaa gcatgggcaa aagataagaa atacgaccgt ctgggctctg
    28441 aagacacgac cgttgaaatc gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa
    28501 caggacgaaa cgttgcaatc atcattgaag tagccgcgat gaatttccgt gcacgtacaa
    28561 tgggctttga cgcaacgaaa acatttgaag aaagattaag tcgcttgatt gaagaaaact
    28621 caggcaacga ctagcaagga gtgacaatat gttagcacaa atcaatcgag ttgcttttga
    28681 gcttgtaggg attcctatct actggtatgc attgatcatc gtatcaggta tcattatcgc
    28741 tatgtggctt agcagcagag aggctgttcg ggtaggatta aaagcagatg atgtgacaga
    28801 ttttatgttg tggggattgc ctatctccat tattggcgct cgcttatatt atgttttatt
    28861 tgatcttccc cagtatatag ctgatccaat ccaaatattc aatatccgtt caggaggctt
    28921 agctatttat ggcggactga ttgcaggcgc gatcacgctg tattttttta ctaaatatca
    28981 ttttatttct ttgtggactt ttttagatat cgctgcacca agtgtattat tagcacaggc
    29041 aatcggtcgt tggggaaatt tcatgaacca cgaagcatat ggaccagaca cgacgcgaag
    29101 cttcttagaa agtctgcatt taccgcaatt catcattgat aatatgtata tcgaagggat
    29161 ctatcgtcaa ccaacatttc tttatgaatc actttggagt ttgattggat ttattttgct
    29221 tttgttttta cgaaagaaac cacacctttt gaaaaaaggc gaagtccttt tatgttatct
    29281 aatgtggtat agtttcgggc gtttctttat tgaaggatta cgtacagaca gtctttactt
    29341 tttaggtgtg atccgtattt ctcaattatt atctgctgtg ttatttgtcg gtacattgat
    29401 tctatttatt tggcgcagaa aaaaaggaaa tttaccggat tatgaccgct cattaggaaa
    29461 aaatcaacaa attatctgat ttttagagcg ttaaaaatga aaaaaatttc aggagtggta
    29521 aaatgaatta tagagtagcg gttttaggac caggctcttg gggtactgct ttggcacaag
    29581 tcctcgccga aaacggacat gacgttcgga tttggggaca tcgtgcttca caagtagaag
    29641 aaatcaacac ccaacacacg aatcaacgtt atttaccaga tcattatcta cccgattcca
    29701 tccaagcatt caccaatatg gaagatgcag taaaagacgc agatgcggtg ctattcgtca
    29761 ttccaacgaa agcaatacgt tctgttgcaa aagaactagt gcctaaattg aatacaaagc
    29821 cagtgatcat ccatgcaagt aaaggattag aacagggaac acataaaagg atctctgaca
    29881 tcttgttaga ggaaatccca gcagaaaaaa gaaaagctgt tgttgttctt tctggaccaa
    29941 gccatgcaga agaagtagca aaacatgaca tcactacgat cacagctgca agtacagatg
    30001 caaaagcagc aaaatatgta caagagctgt tcatgaataa ttattttcgg atctatacca
    30061 atcctgatgt gatcggtgta gagatggggg cagctttgaa aaatattatt gctattggtg
    30121 ctggcgctat tcacggctta gggtttggcg ataatgcaaa ggcagcaatc atgacgcgcg
    30181 gattagctga gatcagccgt ttaggtgtag caatgggtgc caacccgctg acctttatcg
    30241 gattaagcgg aataggcgat ctagtcgtta cttgcacaag tgttcattct aggaactggc
    30301 gcgcaggcaa cttgttagga caagggcagc ccctagaaga ggtgctagcc aatatgggaa
    30361 tggtcgttga aggtgtgaat actacccaag cagcgatgga actagcccaa atgatggaaa
    30421 tcgaaatgcc catcactcaa acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa
    30481 cagccgcaaa agaaatcatg ttacgtgatg gaaaaaaaga aaatgaattc tcattaacaa
    30541 ttttttgaag gagcgaatgt atatgaaagt taaaaaagca gttattccag cagcaggatt
    30601 aggaacaaga tttttaccag caacaaaagc tatggcaaag gaaatgttgc caattgttga
    30661 taaaccaaca atccagttca tcgttgaaga agcgttgaaa tcaggtatcg aagacatctt
    30721 gatcgttaca ggaaaagcta aacgaccaat cgaagatcac tttgattcaa atattgaatt
    30781 agaaacaaac ctaagtgaaa aaggaaagac cgaattattg aaattagtag aagaaacgac
    30841 agatgtcaac ttgcatttca tccgtcaatc tcatccaaag ggtttaggac atgccgttct
    30901 tcaagcaaga gcctttattg gaaatgaacc atttgtcgtt atgctaggcg atgatataat
    30961 ggaagacgaa gtaccattga cacagcaatt aatcgatgat tacgaacgta ctcatgcttc
    31021 tacgatcgcc gttatgcaag ttcctcatga tgaaacttca aaatacggaa ttatcaatcc
    31081 aggagaagta ttggaagatg ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc
    31141 aagcaaagca ccaagtgatt tagctattat tggacgttac ttattgactc cagaaatttt
    31201 tgatgtattg tccgagcaac aaccaggagc cggaaatgaa atccaactaa ctgacgcaat
    31261 cgatacattg aataaaacac aacgcgtatt tgcccgtaaa ttcacaggta aacgttacga
    31321 tgtcggtgac aaatttggtt ttatgaaaac aagtattgaa tatggtctaa cacatccaga
    31381 agtaggtgca cctttacgtg agtacatcct gtctttaggt aaaacattag aagcagaagg
    31441 aagcaagatc gatcataatc caaaaggaat aacagaaatc aaagaagaaa agaaaaaata
    31501 accctttttt ctaacgagct tcagctgcta attgaataaa aagtagttga agctcgtttt
    31561 tatttgttta aaaaacacga aaatgctatc cttatagaaa agagagacaa gaagggagtt
    31621 tgatcatgtc aggaggagaa attgcaggat tgatcgcagc aatcgccttt gcgatacttg
    31681 tcgtttttat cgttcgtgtg ttgttgcaag tttctaaaac tttagaaaaa gtagatcaga
    31741 ctgtagcaga agcaaatacg acgatcgaaa tcgtcacaaa agacgtagat cttttgtcta
    31801 gacaagtaga aggcttactg gtcaaaagta atgaactgct cttggatatc aatggaaaag
    31861 tcgcaacgat cgatccattg ttcacagcag ttgctgattt aagtgaaagt gtatcagaac
    31921 ttaatcattc tagtaaaaac atcgcgacga aagtcggcgg aatcggtaaa acgacagcta
    31981 aagcgacggt tgccggaaaa gtcggtggga cagcgatgaa attttttaaa aacaaaaaac
    32041 atactgaaac gacaggagga aaataaaaat ggctaaaaaa ggtggatttt tattaggtgc
    32101 agtagtcgga ggcacagccg cagcaatcgc cgcgttattg ttagcaccaa aatctggtaa
    32161 agaacttcgg gatgaattag ctgcccaagc ggatgatttg atggatatgg cttcagatta
    32221 cacagatatg gcgaaagaaa aatcgactga gttaacaggt attgcaaaag aaaaagcgca
    32281 agatctttct caacaagcag gtgatctagc aggtagtgtc aaacaaaaag taagtgaatc
    32341 agcagatgaa gcaggaagtg aatctgacga tatcttgtta aatcttaaag aacaatcagc
    32401 tgacttatct aatcgtttca aaaaaacggc agaagatatg aaaaaacctg cagatgaatt
    32461 aggaagtatt gctaaagata cttcagaaga tatcatgatt gatgtaaaag acgctgctgg
    32521 taaagcagga gatactgtca atgaaggaat gtcagaagca aaaccagttg cagatgactc
    32581 aaaagaaatg atgaaaatgg caaaagaaga tgcgaaagaa gctaaaaaag aaataaaaga
    32641 tacaatgaca gattcatcta caaatcacta aaatcacaaa tttatagaat aaaaaggtgg
    32701 gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa ttcttttggt
    32761 gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc gatacgtacc
    32821 cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc ttcttcgatt
    32881 actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc gatcccgtga
    32941 cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt tcgtgcgatt
    33001 tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc aagctgtgct
    33061 tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata agcaattgta
    33121 cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa cacaagttca
    33181 ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt tgctcctgca
    33241 aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg gtattcaatt
    33301 tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc aatctcaaat
    33361 gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag agttttgatc
    33421 agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc cgtcaagata
    33481 tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc tacattgcca
    33541 ggctgaccat agcgagcacg aagttctttt ccgatgatct cccaagggtt ttcgctgtct
    33601 aaatagccac ggacatcata cgcccaacta ctgttttgag catcttcaac ttccaaagca
    33661 ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac acgctcatga
    33721 ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat ataagccaat
    33781 tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt attcatttgg
    33841 caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt attaaaagac
    33901 caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata aaacggttgc
    33961 aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt cagaaaacat
    34021 ataaaatatg aataggagaa cacaaatgga aaagcaaaca atcactatct atgatgtagc
    34081 gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca atccaaacgt
    34141 taaaccagca acaagaaaaa aagtcttgga agttatcgat cgcttggatt atcgtccaaa
    34201 cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggtgtaa tcatccctga
    34261 tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg caacaatgta
    34321 caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag tcaatgtatt
    34381 gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc atatcacaga
    34441 cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg gctctattga
    34501 tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa aagatgcagt
    34561 aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg cgttgatcga
    34621 tcccatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg aaaataacct
    34681 accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg gtctatctct
    34741 agtcgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg atgaattagc
    34801 catcggtatt ttggatggtt tattagaccg tggcgtgaaa gtaccggaag aattcgaaat
    34861 catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa caagtatcac
    34921 acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa tgatgaataa
    34981 agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa aaggatctac
    35041 aaaataataa aaggacaacc attcagaggt tgtgtgcctg ccgtttaact ctgcgttaca
    35101 aggagcaatt tcaaaaaaca gcatctcatg tttgatgaat attgtgactc gtgattcgga
    35161 gttggcagga gaacaccgtt tatttgggaa aaggggttat gacaaaattt tgttataatc
    35221 ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc gtatataaaa
    35281 caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag ggactgtgac
    35341 aatactattg tcacaatccc ttattactgt gtattctatt aaatcaatca tctttctcat
    35401 ctgattcttt agttgcttga ttcgctcttg cataagctga agcggtgttc cagtaatctg
    35461 tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc ggtccatttt
    35521 tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta ttgacagtaa
    35581 cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca tcagatgata
    35641 attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct tgattgatca
    35701 gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca gtattgtcat
    35761 cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc catgaatctc
    35821 ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga tttccgttta
    35881 agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca ctacgcatca
    35941 tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg ctttcgtgtt
    36001 tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct tcttcataga
    36061 cgccgccgtt tgctaaagcc tgaaatccgt tggtctgttg aagcgtcgtg acatctactg
    36121 tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc atctttgcca
    36181 agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct tgatataagt
    36241 gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg aaggtgttcg
    36301 atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct tgccaagttg
    36361 ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt gcaggtccat
    36421 aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag gcatggttat
    36481 tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca gttctgttat
    36541 ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac atgtatccat
    36601 aattttcaac gccattttgc aatgcagcat agacatcttt atcgattgtc gaatggattg
    36661 tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca ctgaccgatg
    36721 aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt cttgttgctt
    36781 cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt tgttgtggta
    36841 gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc gtaatttggc
    36901 cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca gaaagatctt
    36961 cttttagcgc gcctgtgttc gtgtatggac tatacgtgat cgggctttgg ggtaatccag
    37021 cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag attctttttg
    37081 cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat ggtgagacat
    37141 tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt gcagaaagaa
    37201 tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc tgtttgacta
    37261 attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca gatactaatg
    37321 ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca tcctctgtgc
    37381 tgataattgc agttttcaat aaaggtgaaa tcttatcaga tgaaatagtc gtgcgcatca
    37441 gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt gacgtttctg
    37501 taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg tcttcgacga
    37561 gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc aaaagaagaa
    37621 cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga acaaaccatt
    37681 tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa agggtcacct
    37741 caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa cagacaaggc
    37801 tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg atctttatta
    37861 taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag aacagggcaa
    37921 agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat tgttagtaac
    37981 aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag catgagcgga
    38041 aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc gacttttatg
    38101 atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat gaatattgat
    38161 gccggaaaag cgtacatggc agaaaaaggt ctaacaaata tcgtcgttca tgccccttat
    38221 atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt ccaatttctg
    38281 agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact tcacccaggt
    38341 gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg cttgaatgaa
    38401 gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg taaaggtacg
    38461 gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa attgaatgaa
    38521 aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa tattaaagaa
    38581 gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg gcttaaagta
    38641 ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca cgccaatatt
    38701 ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc aatgtttgac
    38761 aatatttcga aaatcttgga aactccctat gtaggcaaag ataaaaagaa agcttacgca
    38821 ccatatggaa aagaaatcgc catgttcaaa gcgcaaacat tcgatccaga agtgtttcct
    38881 gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag caaaaaagga
    38941 ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtacttt ttgcagtctt
    39001 tttcatcatg catcttctcc cccctctttt taaatttaga caaacaaggt atactggtag
    39061 aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg aaggacgaaa
    39121 agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat acatacttta
    39181 ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt aaagcaatct
    39241 atggaacaga catgtcggca acctatgatg accagctgct gattcgctat gctcaaaata
    39301 ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa ggaatctcct
    39361 acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt ttgttcggca
    39421 tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatt gtgaagaaca
    39481 agtatcttct tttaagtatc tatttttatt ttttgttttc tccgattagt tttacacatg
    39541 aatattccac tcgaacatat cgtaatgccc tcgtgattcc ggctgttgag atcattatcg
    39601 cttgtttatt agctatttat taccgcaaat acggttctag cagaaaattg ctgccatggt
    39661 ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat tctatctggc
    39721 ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg ctagggcaag
    39781 acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc tcgaaacggg
    39841 aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc gtctatcaat
    39901 ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac cgttccagtg
    39961 gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg aacttgaata
    40021 aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta gccgcatctc
    40081 ctacattcca tactgtttct acccgtattg acgaattata tacgactcac gcgtggactc
    40141 aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg cgagatgtgg
    40201 taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt tggaaaaaag
    40261 tcaaccaaga attagaagaa gcctatcaca agggaacaat caaaaagaaa aaagagttct
    40321 atctgatggg atcaggagat ggaaagacga tgagtgatct gccaatcgta ttcgatttcc
    40381 ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga ggaaattata
    40441 gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt ccgctaatgg
    40501 agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca aaatcagcaa
    40561 aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg ctgattgctt
    40621 caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct gatcgtgcct
    40681 attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg gtttttcttt
    40741 tcggtgtctc atggttttgt tcttatgcgc cagaaaaaaa ggactatttt ttaagtgtct
    40801 acactggtgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg ctagttttca
    40861 gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg tagctagatt
    40921 tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg atttcttcta
    40981 ccgtttcctg caaaagctgt tcatattttt tgtttccttt atataaatca ccaatcgtcc
    41041 atgccaacag gagaaggaca agaacaatga ttacataagc gatgtgatca gccaatgcta
    41101 gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt aatcctcgta
    41161 agttcaagta agttagacta atcacagaga accagccaag aagttttacg atgaatgaat
    41221 ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa ggaatcatcg
    41281 aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta ttcaaagcgg
    41341 catgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat acaggaatca
    41401 cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata tggatgaatc
    41461 cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg ccagaagcaa
    41521 gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca atcaaaatac
    41581 cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag gaaggatctt
    41641 ggacagaacc ggttttgaag acagcgacac ccatgatcaa tagcagtgca ttgactagaa
    41701 aagccattga caactggata ttagaatccc aagtagaaaa acgtacagct tgtgctacat
    41761 cttcttcgtt ttggtgatcg atcttccgag tttgtgaaac tgctgaatga agatataagt
    41821 tatgcggcat gactgttgca ccaataatcc ctaaagctcc agtcagcgga gtttcgccgc
    41881 cgatgaccgg tgagtctgag aatgtatgac tgttagggat aaagccagca aataaactgt
    41941 gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg acgaaaatta
    42001 agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt aagagaacga
    42061 aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa agtaagtaga
    42121 gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct aattcagtca
    42181 agatccataa aatgacacct aatgtcttac ttgtgcgtgc tcggattgcc tgtgccaaat
    42241 ccatttggct cacgattcct aattttgctg ccatatactg taaaagcata gcaatcagac
    42301 tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg gtgatcgaag
    42361 tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt ccagagtaag
    42421 caagaagtgt tttaaaaaaa cttttcccct ttggtacatc aactgttcca tttacttcag
    42481 ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt ttattggatt
    42541 gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta cacctcaaaa
    42601 agaaaaaatt aaagaaaaag attcggtacg cctaattttt tttcatggcg cgtcttgcta
    42661 gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca tgaaactaaa
    42721 gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga gcaattcgga
    42781 aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt gctattattg
    42841 ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg aaagactatt
    42901 tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg tcaatggggc
    42961 aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac caagggaaaa
    43021 aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt ttttatttcg
    43081 taatttgtta gactattcag attattcagc aaaaaagaca agcacagaca atcctaggct
    43141 cttgataaag acaaaggagg agtaaaatgt cgatttttag aaaattaggc tggttttttc
    43201 gtcaggaaaa gaaacattat ttaattgggg ttttctcgct attcgcagtg gccttggtac
    43261 agttagttcc tccaaaagtg attgggatca tcattgatga gatcgccgat aaaaatttat
    43321 caataaagat cattttcggg tggatcttcc tattgattat cgctgctttt ttacaatatc
    43381 tttttcgtta tatctggcgg acgaatattt ggggaagtgc agctcgattg gagaaagaac
    43441 ttcggcagca gttattcaaa cattttacag aaatggacaa tctcttttac caaaaatatc
    43501 ggactgggga tttgatggct cacgcaacga atgatttgaa tgcgatccag aatgtagcag
    43561 gagctggtat cttgaccttt gcagattcag tgattacggg cggaacaacg atcattgcga
    43621 tgattttgtt tattgattgg cgcttaacac tgattgcgct gattcctctg cctttgctgg
    43681 ccgtcacttc acgagtgctg gggtcaaaac tccatgatgc ttttcgtgat tcgcaagaag
    43741 ctttttctag tatcaatgat aagacacaag aaagtatcac tgggatcaaa gtcattaaaa
    43801 catttggaca agaaaaagaa gatctagcgg attttgaaga aaagattgaa gatgcaatta
    43861 ttaaaaacaa acgagtcaat tttttagact ccttatttga tccattcatt accttgatta
    43921 tcggtctgtc ttatgttgca acaattattt taggtggaaa gtatgtcttg gaaggcacga
    43981 tctcgatcgg gcagatggtc tcatttatta gttatatcgg tatgcttgtt tggccgatgt
    44041 ttgcgattgg tcgattgttc aacgtattgg aaagaggaaa tgcttcttat gatcgcgtca
    44101 atgaactttt gcatgaaaag acgcatatcg tggaacaact gggtgcagtg cagaccccgg
    44161 caaaaggcga gctgaatgta gctatttcct cttttaatta tccgaaagaa gaatcaccag
    44221 cattagaaca aatcaagttt gcgatccaag aaggagaaac attaggtatt gttgggaaaa
    44281 ctggtgcggg aaaaacgacg atcatcaaat tactgatgcg tgaatacgat gattatcaag
    44341 ggaaaatcca atttggcgga cgttcgatca aagaatattc attgaatgct ttattaggat
    44401 caatcggcta tgtgcctcag gatcacttct tgttttcgat gaccattcga gataatatcc
    44461 gtttttctaa tccaaaactt tcagaagaag aagtagagca tgcggcttct ttagcattta
    44521 tcaacaatga aatcaaggaa ttaccaaaag gttatgatac cttagttgga gaacgtggag
    44581 tctctttatc tggcggacag aaacaaagga tttcgattgc tcgagcattg atcaaaaatc
    44641 ctgaattatt aatattagat gatgcactct ctgcagtaga tgccaaaaca gaagaagcta
    44701 tcttgcataa tttaaaagaa ataagaaaag agaaaacaac tatcattgca gctcaccgtt
    44761 taagtagcgt gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa atcattgaac
    44821 gaggaaccca tcatgagctg cttgcactag gcggctggta tgaacaaatg tgggagaaac
    44881 agcaattaga agcgaagata gaaggaggcg aggcctgatg gatgaaaatt atcaatcgga
    44941 atggacaaaa tcgattccac ttaaagaaca gaaaaagatc atcaaacgac tgatgaagtt
    45001 cgccaaacct tttagaagaa cgttcatcgt tgctatctta tttgcgtttg ctttgtctgt
    45061 gatcaatgtc ctattgccaa ggatcatcca aacttttatg gatgatcatc tggctaaaca
    45121 gtcggctacg acacaagtta ttttgttttt tgctggtatg tacttatttg gagtcatagt
    45181 aaaaagtatc atttggtttt tccaatggta tttgtattcc atggcctcat tgaagaccta
    45241 tcagtatatt agggtaaaat tatttgaaaa gctgcataca ctaggtatgc gttattttga
    45301 tcagacaccg gcaggatcaa ccgtttctcg agtgacgaat gacacagaaa cgctatttga
    45361 attttggtac gtctttttga tggtattgac tgggatcttt gcagtcgttt cttcattcct
    45421 tgccatgttt caaatcaatg gagaaattgc cttgtggact ttggtgtttt tgccgatttt
    45481 aggaatcgtc atctggtatt accagaaatt cagttctcgt atttaccgaa atatgcggga
    45541 aaaattaagt cagctgaaca cgaaattgaa tgaatcgatt tccggcatgc gtattatcca
    45601 gcagtttcgc caagaagctc gtttagctaa agaatttgaa caagtgaaca atgagtattt
    45661 agacacgcgt tatgcgatga tcaaaacgaa ttcgattctg ttaagtccga tcatcaattt
    45721 tctatatgca ttggcgattg cgttggtgtt gactttgttt gggatcgatg cgttgcattc
    45781 tcccgtagaa gttggactga tctatgcatt tacgacctat gtccagggat tttttaatcc
    45841 aatgtctcaa atgatggact ttttgagcat cttcacagat gggatggttg ctggtagtcg
    45901 tatcttgaag attatggata cggaagaata tgcaccacaa caaaaccctg gagcagatca
    45961 aatgattact tcaggtaaga ttgagtttcg caatgtaagc ttttcttatg atggagagca
    46021 caatgtatta aacaatatct cttttgttgc taatccggga gagaccgttg cattagtggg
    46081 acacaccgga agcggaaaaa gctcgatcat caacgtattg atgcggtttt acgaatttta
    46141 tgaaggtcag atattgattg atgatcatga cattcgagaa tatccaatgg aagagttacg
    46201 taagaaaatg gggctggtct tgcaagatgc tttcttgttt tatggggata tcgcaggaaa
    46261 tattcggatg atgaatccag ctatcacaga cgaacagata cgccatgcgg ctgagtttgt
    46321 tcaagcaaac aaattcatcg atgaattacc aaaaggatat cactcaaaag tgatcgaaca
    46381 tggtgccagc tattctagtg gacaacgaca gctgatcact tttgcgcgaa caatcgtgac
    46441 tgatcctaaa attctagtat tggatgaagc aacagcaaat atcgatactg aaacagaagg
    46501 attgatccaa gaaggattgg caaaaatgcg tcaaggacgg acaacgattg cgattgccca
    46561 tcggctgtca acgattcgag atgcagaatt gattttagtt ttagacaaag gtcatatagt
    46621 agaacgcggg aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag atatgtacaa
    46681 actgcaaagc agtggtatat aaaagatgtt gtgacagtag aattttgacc taagcactag
    46741 agtgaaaata aaacatagct tctcatattt ttcatctgtg agagttagtg tcgtagtcac
    46801 cagatttaaa aaaagattcg acagctagtc agaagtgcct aatagccact aagaaagcgt
    46861 tgacaaaagc atttgctttc gataatataa agaaaacgat acaacttgga tagtgattgc
    46921 ctgatgcaag ctaaacctga tgagtctccc atggtttcat ggacggcttt ttgggttttt
    46981 ctttttgttt tggatacagc cctatacatc atggaaagga gcaaaacatg ctgattgaga
    47041 aagtactgaa taacaacgtc gtaataacca gggatgaaag aaatcaggaa atgatcgtga
    47101 tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg
    47161 acaaaaaatt tcagttgaca aatgacgggc tttcacaaca gttccaagaa ttggcacaag
    47221 cgattcctct tgagtactta gaaatttctt gtgaaatcat tgaatataca aaagaggctc
    47281 tacaagtcca gttgaatgat tctgtgtata ttgcattgac ggatcatttg tatacggcta
    47341 tcgaacgtca caaaatggga atatttgtac caaatgtatt gctgtatgat atcaaacaac
    47401 tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa gaaaatctcg gataaatacc
    47461 acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag
    47521 gcattcaaag ccagcaagac aatacctacc tgtttactat aacaatacag gatattctaa
    47581 atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat
    47641 tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg
    47701 gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat acgtttggtt
    47761 gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg tccacggacg
    47821 aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa
    47881 aaaaggagga taaattaaca agaacgtatg aaataagggt agtgacattc agttggctaa
    47941 accttccact aataaattgg agggaataga aatgggaaaa taccaagaat tagcgagaaa
    48001 aatcgtagaa aatgtcggcg ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag
    48061 gcttcgtttc aaattaaaaa acgaagaaaa agcaaatgat gagatcttga aaaatatgga
    48121 cggtattgta acggtaatgc gagcgggagg gcagtatcaa gttgtcattg ggaatcatgt
    48181 gccagtcgtg tttgaagagg ttataaaagc aggaaacctt acttttgatg aggctgtttc
    48241 aacagaaaaa atgagaccgt ttgatatgct tattgatgga atatcgggct gtttccagcc
    48301 attccttgct atcctagctg ctggcgggat gattcgtgga ctgacagcat tccttgtctt
    48361 cttaggcgcg tttgaccgcg gttctggcac atttgttatg tttgataata tcggtgattc
    48421 tgtattccaa tttatgcctg ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa
    48481 tgaattcgta gggatgttgc ttggcgcagc attaatgaat cctagcttgt cgctggaagc
    48541 tttgagtggt gcagcagaag caccactaac gaccatattt tctggcacta tttttgaagc
    48601 acctatttat caaacagtat tcggcattcc ctggattgcg cgtaactacg cttcttctgt
    48661 cattcccatt atctttattg tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt
    48721 agtacctgag atgatcgcga atttcttcgt tccattcttc accgttctta ttacaatgcc
    48781 tttaggtttt ttactcgttg gaccagtatt cacattcgca actgatattt taatggctgg
    48841 atttgaaaca cttttagcac tttcgccagt tatttatgga gcaatcgtag gcttcttctg
    48901 gcaaattctg gtgatgtttg gtctccattg ggcaatcgta ccaatgggat tgatgcaatt
    48961 ttctgtgaat ggttggcaaa atattatgac acctgttgca gtagtgtctt ttggacaaac
    49021 tgcagcactt accgcactct actttaagct tagaaatcca aaagacaaag caattgcaat
    49081 accagccatt gtttctggta ttgtggggat tacagagcca gctatttatg ggtttacatt
    49141 accacgtaaa aaaatattta tttttacctg tgtcggtggt gcgttagggg gcgcgtatag
    49201 tgggttaatg aatttaactt cttggaatca aggtggacta ggcatcttta caattcccaa
    49261 ctatattaga ccagatggtg atttaacaga tgtgattaat gtattgattg ggatcgccat
    49321 tgcgatggtg gtatccttta cgctgacttt tttcttttgg aaggatgaag caggtgaaac
    49381 agatgatata cagaaaaaat caggtaaaga aattgtaaaa acaccgatac aaggacaaat
    49441 cgctccacta aatgcagcaa aggatgcagc ttttgcacaa ggaacactag gtcgaggaat
    49501 cttgatctac cctgaaaaag gagaagtacg tgcccctttt gacggaacag tcatgaccct
    49561 gttcccgacg aaacaggcaa ttggaatggt ttctgaaaca gggctggaat tgctgattca
    49621 tgtcggattg gatactgtgc agttggaagg gaaatatttt gaaagcttag tgcagcaagg
    49681 ataaagagtg aaacaaggcg atctgttgct acgcttcgac aaaaagaaaa tcgaaaagga
    49741 aggatacagt ttagaaacac ctgtgatcgt caccaattac acggattatt tggatatttt
    49801 ggaaaaccaa ggggagacgg tcgggccaga cgatgcactt atcaccgcac tgacctgaac
    49861 ggttaggtga agaatggact aggcttaact attcttttta ttccacttct ttattttcaa
    49921 aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcgaat ttttgaccga
    49981 cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa ctatataaca
    50041 cgtctagaag ataacaaatt gatgtattga tcgtaaaatt accaattttt gaatacagtc
    50101 gctcgcgtgt cgtaagtcgc aaagcggctg tactcattgc agatagcgtt gaatcaccga
    50161 tactggtcaa agctaaaatt ggaacatgat tttgtttcaa tatttctgcg gtacgaagga
    50221 tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc gtgcaattgt
    50281 acgcttcata gaatgattca ccaaaagtgg tgacaatcgt gacatccatt ttgatgcgct
    50341 tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg aaaatcacga
    50401 ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga tgaatgagag
    50461 aaagtgtatc atgaatcgtc atctgttcta aagaagccat tttatttgcg atcgtcataa
    50521 taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc ccttcaagat
    50581 aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa cccatttttt
    50641 ttgctacacg gatgagtgta gaggggtgga cataacatcg ctcagcaatc gcttggatgg
    50701 tcatttcgcc aagtgctgga gaatcgctta agagaaactg gatcacttca ttttctgaag
    50761 gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct tcttcttatt
    50821 tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat actatataac
    50881 ttaaaataga cattttttga caagatattt acaaaaatcc actagaaaac agtaaatttt
    50941 gtaaatgtta ctggaaagaa acagatttct attgtttgtt ttgtttacaa aaaagataat
    51001 gaaagtgtaa aaaagtaaag gagtgagaac atggcaaaag gaatcaaaat cgctacgatt
    51061 ggtggaggca gcagttacac ccccgagttg atggaaggat ttatcaaaag atatgaggaa
    51121 ttaccgatac gagaaatctg gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc
    51181 gttagtcaga tgtctcagcg tatgtgggat gcttctccgt atgaagtcaa gatctatgca
    51241 actctggatc gggaagaagc attgaaagat gcggattttg ttacgactca gtttcgtgtc
    51301 ggtcaattag atgcacgaat caaagacgag cggatcccta gttattatgg gatgattggg
    51361 caagaaacaa atggtgctgg cgggatattc aaagcttttc gtaccgttcc agtcgttttg
    51421 gcaatcgttg aggacatgaa gcgtttatgt ccaaatgctt ggctgatcaa tttcgcgaac
    51481 ccaagtggga tggtgacaga agcagttatc agatatggca aatgggataa agtgattgga
    51541 ttatgcaatg tgccagtgat ggcgatgatg acagaaccag caatgcttgg caagaaaaaa
    51601 gaagagctga tctacaagtt tgccggtcta aatcatttcc attggcacaa agtagcagat
    51661 aataaaggaa atgaccgcac ggcagaattg attgatcgtt tatacgatga agagaatgga
    51721 ttaccgaaaa atatctttga tatcccgttt ttcaaagaac aattaaaaca aatgaacatg
    51781 atcccttgtg gctaccaccg ctattactat cgtgaagaag aaatgctgga acatgcttta
    51841 gaagaatatc aaacgattgg tacaagagcg cagcaagtga aagaaacaga agcagagcta
    51901 tttgaattat acaaagatcc agaattagac cataaacctg aacaattgca aaaacgaggt
    51961 ggcgcttatt attcagacgc tgcctgtgaa acgattgctt caatctatgg aaataaagaa
    52021 acacagatcg tggtatctac aaagaatgat ggagctgttc ctgacctgcc ggcagattgt
    52081 gtagtagaag tcactgccta tgttggcgca caaggagcga gaaatgtagc gtttggttct
    52141 cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga atatggaatt gctgacgatc
    52201 gaagcagctg tgactggtga ttatgggaca gctttacagg cgtttacgat caatccactt
    52261 atacgatcag gaaagacagc aaaacggatc atggatgagt tgtttattgc acaccagcag
    52321 tatttaccgc aattcaagga gacaatcagc cgattaaaag aagaaggaat cgaagttcaa
    52381 gatgaactgg caagagaatt atcaggtaat acacatataa tggaggaagt ataatgggat
    52441 tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa tcaatgtgag ggcggttatg
    52501 atgaaggtgg acgcggacta gcaaacgtag atgttgtgcc aatcggaaaa gaccgttttc
    52561 ctgttatcac cggaaagaaa aaaatgtttg aatttgatga ggatcatttt tatccagcaa
    52621 aaattgcaat cgatatgtat catcgttata aagaagatat tgctttgttt gcagaaatgg
    52681 ggttcaagac ttatcgaatg tccatcgcct ggagtcgtat ttttccaatg ggggatgaga
    52741 aagagccgaa tgaagctggc ttgaaatttt atgaggatgc ctttaaagaa tgccaaaagc
    52801 acggaattga gccacttgtg acgattacgc attttgattg tccaatgcat ttgatcaaag
    52861 aatatggtgc gtggcgttcc agagaactcg ttggttttta tgaaaatctg tgtcgagtca
    52921 tcttcaatcg ctataaagga cttgtcaaat attggctgac attcaatgag atcaatatga
    52981 tccttcatgc accattcatg ggcgcagggc tgtattttga aaaaggagaa aatgaagaac
    53041 aaatcaaata tcaagcggca catcatgagt tggttgcaag tgccatcgca acgaagattg
    53101 ctcatgaagt agatccggag aacaaagtag gctgtatgct ggcagcagga gcatattatc
    53161 catacacttg caatcctgaa gatgtttggg cttctagaaa atcagatcga gaaaattatt
    53221 tcttcattga tgtccagtca cgaggagaat atcccgctta tgcattgaaa gaaatggagc
    53281 gaaatggcat tcagatcgaa atggaaccag gggatgccgc attgctgaaa gaacatactg
    53341 ttgattttat ctctttttct tattatgctt cccgagtttc gactaccgac aacgaactct
    53401 tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa tccatatctg caagcaagtg
    53461 aatgggggtg gcaaattgat ccattaggct tgagaataac gatgaatgat atttatgatc
    53521 gttatcaaaa accattgttt attgtagaaa acggcttagg tgcagtcgat acgccagatg
    53581 aaaatggtta tgtggaagat gattaccgaa tcgattacct ggcagcgcat atccaggcga
    53641 tgaaggatgc agtagaacta gacggagtcg atctattagg ctatacgact tggggatgta
    53701 ttgatttagt atcagctggt accggtgaga tgaagaaaag gtacggattc atttatgtcg
    53761 atcgtgataa ccaaggaaat ggcacattag cccgttcaaa gaaaaaatca ttcaactggt
    53821 ataaaaaagt aattgattca aatggagaaa atctttcgaa cgattgatca atgatttata
    53881 aaagtctatt gtttttcagc ggatttttcg agtataataa agctattcga gaaacgcaag
    53941 cagaacaata gacttttttt cgcattggct gtaaagtatg cttacttgat taagtaaagg
    54001 agaatctaca tatgtatgat gtaatcgtta ttggcgcagg tcctgctggg atgactgctg
    54061 ctttatatgc ttcacgttca aacttatccg tattgatgat cgaacaaggt gcccctggcg
    54121 gccaaatgaa caatacggca gaaatcgaaa attatcctgg attcgattcg atcatgggcc
    54181 ccgatctagc ttataagatg tatgaaagtg tctcaagatt tgggacagaa aatgcttatg
    54241 gaatcgttca agatatcaag gatcacggca gccataaaga agtcatctgt gaagataaaa
    54301 gttacgaagg aaaaacagtc attatcgcaa caggatgtat ccaccgtaaa ttaggcgtac
    54361 ctggcgagga agaatttgct ggacgaggcg tttcttactg tgcagtctgt gatggtgcgt
    54421 ttttccgtaa caaacgtcta gtagtcatcg ggggtgggga ctcagcagta gaagaagcca
    54481 tctatttgac acaatttgct tctgaagtag tgatcgtcca ccgtagagat gaattacgtg
    54541 cacaaaagat catccaagac cgtgcatttg caaatgaaaa aatttccttt atgtgggata
    54601 cagtcgttga cgaaatcgtt ggaaacgaaa tggtagtgac aggtgtgaaa gcacgtaatg
    54661 tcaaaaccgg caatgaattt gatttagcag cagacggagt attcatctat gttggactgg
    54721 atccattaac tgcacctttt agagaagcag gtttgacgaa cgaagctggc tggattccaa
    54781 cagacaacga aatgaaaacg aatatgccag gcgtttttgc catcggagat gttcgtgaaa
    54841 aagatttgcg acaaatcact acagctgttg gtgacggcgg tattgctgga caacaagtgt
    54901 ataaatactt ggagtcttta gaagaaacaa cagcataagt ttaaaaagag tagaagaacg
    54961 ggttcaattg ttcttttact cttttttttc gcgtgaaatt tactgaatct gatttatgcg
    55021 aacacgtcca acaccttttg ataaaagaat cctttcttga tttaagcgtt ttttggtcct
    55081 catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt tcagaaagag
    55141 gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag
    55201 aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg
    55261 ctttttatgc accgttggaa ttcggtactg caggtatgcg gggaatctta ggacctggga
    55321 tcaatcgaat gaacatctat acggtaagac aagcaacaga aggactagca cgtttcatgg
    55381 atacacaagg tgaagaaaca aaacgccgag gtgtcgctat cgcttatgac tcacgccatc
    55441 agtcaccaga atttgcaatg gaagcagcca aaacactagc acatcacaat atcccgtctt
    55501 atgtattcga aagtctgcgt ccaacaccag agctttcttt tgcagttcgt catttgaaga
    55561 cgtttgcggg aatcatggta accgcatctc acaacccagc ggcttacaat ggatataaag
    55621 tctacggaga agatggcgga caaatgcctc ctgcagacgc agatgcattg acgaaatatg
    55681 tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc tgacgaagaa gctaaacata
    55741 gcgggttgat cacgatcatc ggagaagaag tggacgctgc ttatctagaa gaaatcaaag
    55801 cagtgacgat tgatcgtgaa ttggttgaaa caatggggaa agatttgaaa cttgtgtaca
    55861 cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg
    55921 aacaattcgt actagttccc gaacaagctg tggcagatcc agatttcact acggtcaaat
    55981 cgccaaatcc agaagaacat tcagcttttg aatatgctat acgacttgga gaaaaagaag
    56041 gagctgactt gctgattgca acagatcctg atgcagaccg tttaggcgca gcggttcgtt
    56101 tgccagatgg ttcttatgaa gtattgactg ggaaccaaat cggagcattg tttgctcaat
    56161 atattttgga agcacatcaa tcaagaggca ctttaccaga aaacgcagcg gtattgaaat
    56221 caatcgtttc cagcgaattg cctacagcaa ttgcggaaca ctacggtgct accatgttca
    56281 atgtgttgac agggtttaaa ttcattgctg aaaaaatcca acaatttgaa gaagaccatt
    56341 cacatacttt tatgtttgga ttcgaagaaa gttatggtta tttagttaaa ccttttgttc
    56401 gcgataaaga cgcgatccaa gcattgcttt tattagcaga agtagcagct tactataaaa
    56461 aacaaggcaa aacgttgtat gacggcttgc aaacgatttt tgaaacctat ggctatttcg
    56521 cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa atctctgctt
    56581 tgatggaaaa attccgtaaa gaagcaccga cagaatttgc cggagcaaaa gttattcaaa
    56641 cagaagactt taaagaattg acgagaacag accgtgaagg aaacgtagaa aaactaacaa
    56701 caccgccatc cgacgtattg aagtatacat tggaagaaaa tggctggatc gctatccgcc
    56761 cttcaggaac tgagccgaaa atcaagtttt atattggtgt aaaaggaagc tctacagaag
    56821 acgcagccga aaaagtagcc tcttatgaag cgattatcaa aaagatgaca gaagaataaa
    56881 aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt tcaagttgga
    56941 ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag aaagtcagtc aattattcaa
    57001 aatgttgagt gatcccactc gtcttaaaat tctgctatat ctgaaagacg gggaacaaaa
    57061 tgtgacagca atcacccaag cagtggaaat ggagcagtct gctgtatcgc atcaactacg
    57121 tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag ggaaaagcga ttttgtatag
    57181 tcttgatgat tctcatgtac tggatatttt gaaccagaca ctcaaacatg tggaacatca
    57241 ataacgatag gtgaaaatag ataaacactg gagtgtgaca acgaagaaat ttcgttgtca
    57301 cactccagtg ttttttaaga aaaagacaaa aatgattaat cgcttttttc tgttgtttca
    57361 gttccataat aactatgatc ttctaggtct aaagcattca aaataagcga agccgtttct
    57421 tcaatagata aagatgccac attgatcact acacatccta gtttttcata aagatcattc
    57481 gcaaaatcga gttcagctct aattttttcg atatcggaat aagcagtatc agggtttaat
    57541 ccgtaagagc gcatccgttc tttacggata ttatttaaaa tatctgggtt attcgttagt
    57601 cccacgattt ttttaggatc gatttcccac agctgtttgg gaatatgtgc ttggggaatc
    57661 aaaggtaaat tagcaacttt taaattttta tttgctaaaa acaagctcaa aggcgttttg
    57721 gaagttcggg atacgcctag caaaacgaca tctgcttcta aaaaaccacg aggatctttc
    57781 ccatcatcgt attttacagc aaattccatt gctttgatcc gtttgaaata gttctcattc
    57841 aataaatgga gggcaccagg ttgccgaatt ggcttgatac cagttaaacg ttcaatttct
    57901 gatatcggtg gcgtcatcac atcgaaacta aataattgat gttcttcaca aaaagaacgt
    57961 gttatctcga tcagttcttg attgattaat gtatgcagga caagtgcttt ttcttctaat
    58021 gcatcttgca atgcctgcct taatgttttt tcatctgtca cgaacgttct gcggaatagg
    58081 ttgaactcaa cggtaggata ctgagccata gtagcttgcg ctagttttgt cgcagtttct
    58141 ccagcagagt cggagatgac aaaaaaagta acgatttctg tagaatcgga agacatggat
    58201 aaaacctcac ttgcattttt tttatgttta ttataccata ctctagaatg cgtacttcgt
    58261 aatcattttg atttggagga ataagtcatg aaaaaacaga cattgatcga acaatcatta
    58321 gaaacattga aaaataacgg ttttaaatat actaaaaaaa gagaagcatt gttaacttat
    58381 ttagtgaagc gtaaccgcta cgtatctgct aaagaagttt ttgactatat gaatcaggaa
    58441 ttcaaagggg taagttatga tacgatttac cggaatctcc atgattttgc agagatcgat
    58501 ttactggaag aaaccgattt aaatggtgaa atgaagtttc gttttcattg ctgtcagggg
    58561 gaaatcggcc atcaccatca ttttatctgt acagtgtgcg gaacaacgaa agaaatccat
    58621 atgtgtccaa tgaattattt cgaagaacag ctaggtggat gcacgatcga agggcataga
    58681 ttcgagattt tcggaagatg tgaaaattgt caataaggat ttaaataaaa taacatttga
    58741 taatttaaaa aaaaaagcaa gatttccttg acggtcaaaa cagcattaga tataatattt
    58801 acaggacagt cttgtttttt gagatatatt tcaataacaa ttacggttac tttttaagct
    58861 cggagggagg gaattaacat gtcaaaaaca gtcgttcgca aaaacgaatc acttgacgat
    58921 gctcttcgtc gcttcaaacg ttccgtgtca aaagcgggta ctttacaaga atctcgtaaa
    58981 cgtgaattct acgaaaaacc aagtgtaaga cgcaagaaaa aatctgaagc agctagaaaa
    59041 cgcaaaaaat tctagttctg tgatgaattg gagttggtag tgtgtcacta ctaagtagat
    59101 taaacgatga catgaaagca gcgatgaaag cgaaagataa ggaatcttta caagtcatcc
    59161 gaatgatcaa gtcatctata caaaacgagc agattaaaga aggtcacgac ttgacagaag
    59221 aagaagaact aacggtgttg tctcgcgaaa tgaaacagcg acgtgattct ttgcatgagt
    59281 ttgaagaagc aggacgtgac gatttagctg aaaaagtaaa aagtgaaatt gtaatagtcg
    59341 aaaaatatat gccggaacaa ctttccgacg aagaaattcg acaacttgtt caagaagcaa
    59401 tcactcaaac tggtgcttca tcaatgaaag aatttggcaa agttatgggt gcaattatgc
    59461 ctaaagtcaa aggaaaagca gatggaaacc aagtgaatgc gattgtgaaa gaactattac
    59521 aatcttaatt ttatcattta aactgcggac agtgattgtc cgcagttttt tgtgttcgta
//
LOCUS       DAZUKD010000003        57897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000003 DAZUKD010000000
VERSION     DAZUKD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.003"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(512..1015)
                     /locus_tag="ACG8N9_000125"
     CDS             complement(512..1015)
                     /locus_tag="ACG8N9_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4895762.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1374..2399
                     /locus_tag="ACG8N9_000126"
     CDS             1374..2399
                     /locus_tag="ACG8N9_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4895763.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2722..3600
                     /locus_tag="ACG8N9_000127"
     CDS             2722..3600
                     /locus_tag="ACG8N9_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4895764.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4076..4192
                     /locus_tag="ACG8N9_000128"
     CDS             4076..4192
                     /locus_tag="ACG8N9_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4895765.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5120..5458
                     /locus_tag="ACG8N9_000129"
     CDS             5120..5458
                     /locus_tag="ACG8N9_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895766.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5552..5782
                     /locus_tag="ACG8N9_000130"
     CDS             5552..5782
                     /locus_tag="ACG8N9_000130"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4895767.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6182..6553
                     /locus_tag="ACG8N9_000131"
     CDS             6182..6553
                     /locus_tag="ACG8N9_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4895768.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6827..7519)
                     /locus_tag="ACG8N9_000132"
     CDS             complement(6827..7519)
                     /locus_tag="ACG8N9_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4895769.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7522..8307)
                     /locus_tag="ACG8N9_000133"
     CDS             complement(7522..8307)
                     /locus_tag="ACG8N9_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4895770.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8544..9944
                     /gene="lacG"
                     /locus_tag="ACG8N9_000134"
     CDS             8544..9944
                     /gene="lacG"
                     /locus_tag="ACG8N9_000134"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4895771.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9969..10286
                     /locus_tag="ACG8N9_000135"
     CDS             9969..10286
                     /locus_tag="ACG8N9_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4895772.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10299..11972
                     /locus_tag="ACG8N9_000136"
     CDS             10299..11972
                     /locus_tag="ACG8N9_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4895773.1"
                     /translation="MDGIIKQIEKGKAFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11986..13617
                     /locus_tag="ACG8N9_000137"
     CDS             11986..13617
                     /locus_tag="ACG8N9_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4895774.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14108..15673)
                     /gene="guaA"
                     /locus_tag="ACG8N9_000138"
     CDS             complement(14108..15673)
                     /gene="guaA"
                     /locus_tag="ACG8N9_000138"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4895775.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15732..16655)
                     /gene="coaA"
                     /locus_tag="ACG8N9_000139"
     CDS             complement(15732..16655)
                     /gene="coaA"
                     /locus_tag="ACG8N9_000139"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4895776.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16851..17456
                     /locus_tag="ACG8N9_000140"
     CDS             16851..17456
                     /locus_tag="ACG8N9_000140"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4895777.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17764..18780
                     /locus_tag="ACG8N9_000141"
     CDS             17764..18780
                     /locus_tag="ACG8N9_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4895778.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18921..20222
                     /locus_tag="ACG8N9_000142"
     CDS             18921..20222
                     /locus_tag="ACG8N9_000142"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4895779.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20268..20930
                     /gene="deoC"
                     /locus_tag="ACG8N9_000143"
     CDS             20268..20930
                     /gene="deoC"
                     /locus_tag="ACG8N9_000143"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4895780.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20951..21349
                     /locus_tag="ACG8N9_000144"
     CDS             20951..21349
                     /locus_tag="ACG8N9_000144"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4895781.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21415..22497
                     /locus_tag="ACG8N9_000145"
     CDS             21415..22497
                     /locus_tag="ACG8N9_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4895782.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22709..24271
                     /locus_tag="ACG8N9_000146"
     CDS             22709..24271
                     /locus_tag="ACG8N9_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4895783.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24264..25397
                     /locus_tag="ACG8N9_000147"
     CDS             24264..25397
                     /locus_tag="ACG8N9_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4895784.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25397..26353
                     /locus_tag="ACG8N9_000148"
     CDS             25397..26353
                     /locus_tag="ACG8N9_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4895785.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26452..27618
                     /gene="deoB"
                     /locus_tag="ACG8N9_000149"
     CDS             26452..27618
                     /gene="deoB"
                     /locus_tag="ACG8N9_000149"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4895786.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27642..28460
                     /locus_tag="ACG8N9_000150"
     CDS             27642..28460
                     /locus_tag="ACG8N9_000150"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4895787.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28477..29184
                     /gene="deoD"
                     /locus_tag="ACG8N9_000151"
     CDS             28477..29184
                     /gene="deoD"
                     /locus_tag="ACG8N9_000151"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4895788.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29482..30168)
                     /gene="gpmA"
                     /locus_tag="ACG8N9_000152"
     CDS             complement(29482..30168)
                     /gene="gpmA"
                     /locus_tag="ACG8N9_000152"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4895789.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30340..30921)
                     /locus_tag="ACG8N9_000153"
     CDS             complement(30340..30921)
                     /locus_tag="ACG8N9_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4895790.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31054..31737)
                     /gene="rpiA"
                     /locus_tag="ACG8N9_000154"
     CDS             complement(31054..31737)
                     /gene="rpiA"
                     /locus_tag="ACG8N9_000154"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4895791.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32056..32469
                     /gene="rpsL"
                     /locus_tag="ACG8N9_000155"
     CDS             32056..32469
                     /gene="rpsL"
                     /locus_tag="ACG8N9_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4895792.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32553..33023
                     /gene="rpsG"
                     /locus_tag="ACG8N9_000156"
     CDS             32553..33023
                     /gene="rpsG"
                     /locus_tag="ACG8N9_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4895793.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33117..35204
                     /gene="fusA"
                     /locus_tag="ACG8N9_000157"
     CDS             33117..35204
                     /gene="fusA"
                     /locus_tag="ACG8N9_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4895794.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35376..36563
                     /gene="tuf"
                     /locus_tag="ACG8N9_000158"
     CDS             35376..36563
                     /gene="tuf"
                     /locus_tag="ACG8N9_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4895795.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36970..37278
                     /gene="rpsJ"
                     /locus_tag="ACG8N9_000159"
     CDS             36970..37278
                     /gene="rpsJ"
                     /locus_tag="ACG8N9_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4895796.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37309..37938
                     /gene="rplC"
                     /locus_tag="ACG8N9_000160"
     CDS             37309..37938
                     /gene="rplC"
                     /locus_tag="ACG8N9_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4895797.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37966..38589
                     /gene="rplD"
                     /locus_tag="ACG8N9_000161"
     CDS             37966..38589
                     /gene="rplD"
                     /locus_tag="ACG8N9_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4895798.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38589..38879
                     /gene="rplW"
                     /locus_tag="ACG8N9_000162"
     CDS             38589..38879
                     /gene="rplW"
                     /locus_tag="ACG8N9_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4895799.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38918..39751
                     /gene="rplB"
                     /locus_tag="ACG8N9_000163"
     CDS             38918..39751
                     /gene="rplB"
                     /locus_tag="ACG8N9_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4895800.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39794..40072
                     /gene="rpsS"
                     /locus_tag="ACG8N9_000164"
     CDS             39794..40072
                     /gene="rpsS"
                     /locus_tag="ACG8N9_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4895801.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40095..40442
                     /gene="rplV"
                     /locus_tag="ACG8N9_000165"
     CDS             40095..40442
                     /gene="rplV"
                     /locus_tag="ACG8N9_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4895802.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40456..41112
                     /gene="rpsC"
                     /locus_tag="ACG8N9_000166"
     CDS             40456..41112
                     /gene="rpsC"
                     /locus_tag="ACG8N9_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4895803.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41115..41549
                     /gene="rplP"
                     /locus_tag="ACG8N9_000167"
     CDS             41115..41549
                     /gene="rplP"
                     /locus_tag="ACG8N9_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4895804.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41539..41727
                     /gene="rpmC"
                     /locus_tag="ACG8N9_000168"
     CDS             41539..41727
                     /gene="rpmC"
                     /locus_tag="ACG8N9_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4895805.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41752..42018
                     /gene="rpsQ"
                     /locus_tag="ACG8N9_000169"
     CDS             41752..42018
                     /gene="rpsQ"
                     /locus_tag="ACG8N9_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4895806.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42076..42444
                     /gene="rplN"
                     /locus_tag="ACG8N9_000170"
     CDS             42076..42444
                     /gene="rplN"
                     /locus_tag="ACG8N9_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4895807.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42481..42789
                     /gene="rplX"
                     /locus_tag="ACG8N9_000171"
     CDS             42481..42789
                     /gene="rplX"
                     /locus_tag="ACG8N9_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4895808.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42817..43356
                     /gene="rplE"
                     /locus_tag="ACG8N9_000172"
     CDS             42817..43356
                     /gene="rplE"
                     /locus_tag="ACG8N9_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4895809.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43375..43560
                     /locus_tag="ACG8N9_000173"
     CDS             43375..43560
                     /locus_tag="ACG8N9_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4895810.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43596..43994
                     /gene="rpsH"
                     /locus_tag="ACG8N9_000174"
     CDS             43596..43994
                     /gene="rpsH"
                     /locus_tag="ACG8N9_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4895811.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44026..44562
                     /gene="rplF"
                     /locus_tag="ACG8N9_000175"
     CDS             44026..44562
                     /gene="rplF"
                     /locus_tag="ACG8N9_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4895812.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44723..45079
                     /gene="rplR"
                     /locus_tag="ACG8N9_000176"
     CDS             44723..45079
                     /gene="rplR"
                     /locus_tag="ACG8N9_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4895813.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45100..45600
                     /gene="rpsE"
                     /locus_tag="ACG8N9_000177"
     CDS             45100..45600
                     /gene="rpsE"
                     /locus_tag="ACG8N9_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4895814.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45615..45794
                     /gene="rpmD"
                     /locus_tag="ACG8N9_000178"
     CDS             45615..45794
                     /gene="rpmD"
                     /locus_tag="ACG8N9_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4895815.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45838..46278
                     /gene="rplO"
                     /locus_tag="ACG8N9_000179"
     CDS             45838..46278
                     /gene="rplO"
                     /locus_tag="ACG8N9_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4895816.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46278..47573
                     /gene="secY"
                     /locus_tag="ACG8N9_000180"
     CDS             46278..47573
                     /gene="secY"
                     /locus_tag="ACG8N9_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4895817.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47635..48282
                     /locus_tag="ACG8N9_000181"
     CDS             47635..48282
                     /locus_tag="ACG8N9_000181"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4895818.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48473..48691
                     /gene="infA"
                     /locus_tag="ACG8N9_000182"
     CDS             48473..48691
                     /gene="infA"
                     /locus_tag="ACG8N9_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4895819.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48724..48840
                     /gene="rpmJ"
                     /locus_tag="ACG8N9_000183"
     CDS             48724..48840
                     /gene="rpmJ"
                     /locus_tag="ACG8N9_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4895820.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48858..49223
                     /gene="rpsM"
                     /locus_tag="ACG8N9_000184"
     CDS             48858..49223
                     /gene="rpsM"
                     /locus_tag="ACG8N9_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4895821.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49251..49640
                     /gene="rpsK"
                     /locus_tag="ACG8N9_000185"
     CDS             49251..49640
                     /gene="rpsK"
                     /locus_tag="ACG8N9_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4895822.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49720..50658
                     /locus_tag="ACG8N9_000186"
     CDS             49720..50658
                     /locus_tag="ACG8N9_000186"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4895823.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50686..51066
                     /gene="rplQ"
                     /locus_tag="ACG8N9_000187"
     CDS             50686..51066
                     /gene="rplQ"
                     /locus_tag="ACG8N9_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4895824.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51338..52177
                     /locus_tag="ACG8N9_000188"
     CDS             51338..52177
                     /locus_tag="ACG8N9_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4895825.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52153..53022
                     /locus_tag="ACG8N9_000189"
     CDS             52153..53022
                     /locus_tag="ACG8N9_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4895826.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            53012..53809
                     /locus_tag="ACG8N9_000190"
     CDS             53012..53809
                     /locus_tag="ACG8N9_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4895827.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            54012..54758
                     /gene="truA"
                     /locus_tag="ACG8N9_000191"
     CDS             54012..54758
                     /gene="truA"
                     /locus_tag="ACG8N9_000191"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4895828.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(55213..57882)
                     /locus_tag="ACG8N9_000192"
     CDS             complement(55213..57882)
                     /locus_tag="ACG8N9_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4895829.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        18492 a        10418 c        12396 g        16591 t
ORIGIN      
        1 attttacact tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca
       61 gatttctcta ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta
      121 ggatcaggca gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac
      181 catagaaaat ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct
      241 gagtagggta taaattaaga cggaattcct ttctagattg agatattgac taggaagaat
      301 gttaagattt taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata
      361 gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa
      421 aatagatttt tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg
      481 tgtctattac ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa
      541 ataaaaaagc tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt
      601 ctaacttatt tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc
      661 ctcctgttgt attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt
      721 gaatcaattc tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa
      781 gtctcttgaa attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag
      841 tggtctcacg taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga
      901 cattgctgta agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca
      961 accataatag aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca
     1021 tctcctccat ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa
     1081 gtttaaggac caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg
     1141 gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc
     1201 aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata
     1261 ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt
     1321 ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat agattgttca
     1381 agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc
     1441 tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt
     1501 tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc
     1561 tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat
     1621 cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat
     1681 tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg atgctttctg
     1741 ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt
     1801 ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc
     1861 ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga
     1921 tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct
     1981 tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa
     2041 acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat
     2101 gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca
     2161 cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt
     2221 ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt
     2281 tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt
     2341 gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga
     2401 aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca
     2461 aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga
     2521 actttcaaaa aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa
     2581 taaactttcc tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat
     2641 agaaagttta agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc
     2701 ggataatcag tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag
     2761 aaaataagca atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata
     2821 gagcaaatga ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat
     2881 gaactaatgg atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa
     2941 gaggaacctt ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa
     3001 atttatcaac cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact
     3061 agatttattc atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg
     3121 atttggcatt acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca
     3181 tattttccga aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat
     3241 attccttccg atgcagattt atctcagaat aagataagag atagttacga aagtgccatt
     3301 cgtttttttg aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg
     3361 ttactatcac ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca
     3421 gccgattatc gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt
     3481 ggacgagtag atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca
     3541 aaagagcaat tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa
     3601 gtttagtact tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct
     3661 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
     3721 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttgtttcttg gagttaaacg
     3781 cttctgtccc agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa
     3841 ctagctataa caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa
     3901 acgtaataat gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt
     3961 tttaacaagt tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt
     4021 ttcgatttgt gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga
     4081 aaaaacagat atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa
     4141 acgtgcgctg aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg
     4201 aaacttaatt aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt
     4261 gtgtaaggtt tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt
     4321 agaatctttc ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga
     4381 accacaaagc acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca
     4441 acctcttatt ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt
     4501 gaaacatttt tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt
     4561 tcatgaaata tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat
     4621 aagcagttac gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca
     4681 aaaaaatcag tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac
     4741 gccgaaaaaa tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga
     4801 ataataagtt ttggttttca gataatagtc gataagttgt gtattggaat tttagataca
     4861 gtaataaagt atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa
     4921 attaatggtt aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt
     4981 gtgatactag actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa
     5041 atgaaaaaga agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt
     5101 tagccaagta acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt
     5161 tcgtgctaca gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc
     5221 tagtgagcca attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt
     5281 tatggtggta aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat
     5341 tgaaaaggtg agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat
     5401 cattttaacg gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac
     5461 actagtgaaa tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa
     5521 atcgatcatc aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat
     5581 ttattacgtg aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag
     5641 tcgctactca attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa
     5701 aactgttttt tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga
     5761 ttatgttagt atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg
     5821 ttattgtttt ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt
     5881 atccagagta ttttatttta acttttgtat atttagtaag tagctacaca cttctatata
     5941 aattcagaag ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat
     6001 aatatttttg atttatatag gaaattaaaa atattataga atcaacactc agagaacggg
     6061 ttggttttta atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga
     6121 agttggttac cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat
     6181 aatgagagat tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa
     6241 tgaattatct gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga
     6301 ctatattgat atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat
     6361 aaaagctttt atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct
     6421 agtaggaaaa ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc
     6481 tttgtctatg ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt
     6541 ggaaatcttt taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga
     6601 tgatttttaa gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag
     6661 atagagatga acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat
     6721 ttcactaagg atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag
     6781 gaggtgtgca tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg
     6841 atgtggcaga caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat
     6901 ttagctgtgc ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg
     6961 ttccgatccc cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac
     7021 aatcagccgg agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt
     7081 tcccagggaa agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat
     7141 cgaaaatatc aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg
     7201 gaatctgttg gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct
     7261 cgttttcatt ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg
     7321 cgatctcaga ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc
     7381 gaccatgaat attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta
     7441 tcatatgcag ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat
     7501 caaatataat ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat
     7561 ttgcagatcg acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt
     7621 aatgagcaga tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt
     7681 tttattatca acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc
     7741 tccttcttcg gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa
     7801 tgcttttgta aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc
     7861 ggtatttttc cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag
     7921 aatatcaaaa actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt
     7981 cgccaatagt tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa
     8041 ggaggctgct gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt
     8101 gtgagaaagt tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag
     8161 cagcttgccc gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc
     8221 taattgattg atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat
     8281 agtaagcaat ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca
     8341 tataaacgga caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat
     8401 gtttgttaca atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt
     8461 tgttgacaaa tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat
     8521 tatagatttg aggagagatg tacatgctta aattaccaga agattttatt tttggcggag
     8581 ccacagcagc ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt
     8641 gggatgactt tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc
     8701 atcaatatgc aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat
     8761 ccattgcttg gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg
     8821 ctttttatca tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt
     8881 tgcatcattt tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa
     8941 caatagaagc atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg
     9001 tgtggagtac ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt
     9061 tcccaccagg aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg
     9121 ttgctcatgc ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg
     9181 tagttcactc attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg
     9241 cctttttaga cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt
     9301 attcaactga aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc
     9361 attttcctga agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag
     9421 ggatcaatca ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc
     9481 ataacggtac gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta
     9541 tttataaaga aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat
     9601 atgacttgtt gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg
     9661 agaacgggat ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa
     9721 tcgactattt gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg
     9781 tgaaggggta ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca
     9841 aaaggtatgg cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag
     9901 cttactggta caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg
     9961 aagaaaagat gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag
    10021 gcgacgctcg cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac
    10081 atgttgattc ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa
    10141 caaaaatttt agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc
    10201 atgggcagga tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg
    10261 tactgtacag acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa
    10321 tcgaaaaagg gaaagcattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc
    10381 gtgacggatt tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg
    10441 ctgcggttcc tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga
    10501 aagtctataa ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct
    10561 gcttggctga ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg
    10621 tgatgctggc ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat
    10681 tttcaacgga atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa
    10741 cagttaattt atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag
    10801 ttcctggaac gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg
    10861 cggccgtttt aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa
    10921 tcattacggt gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta
    10981 tctggggagc gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc
    11041 cggcaattgc ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg
    11101 gtgaacaagc ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag
    11161 gaactggggc aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt
    11221 tgaaggcagt tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc
    11281 tatttgcggc acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg
    11341 ttgtaaacgt ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt
    11401 acgttttgcc ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta
    11461 tcttagcttt tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat
    11521 tttgcaaagc atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg
    11581 caaccgagga aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag
    11641 ttatcagcga tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa
    11701 gtgcgatgtt agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg
    11761 caatggcggg tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct
    11821 tagcaccgca agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa
    11881 tcaagctggt tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag
    11941 ctgttcagtt tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt
    12001 tggttgcgta aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa
    12061 actgttgaag catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat
    12121 atggaaaaat ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt
    12181 ttaaaaaata atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt
    12241 aatggagaaa gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa
    12301 cgagcaaaaa atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt
    12361 gatcctggga aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat
    12421 acggcattgt acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat
    12481 cctgatatgg ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag
    12541 tcttggggag aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc
    12601 caagctgttc gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt
    12661 ggcgatacag gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt
    12721 gatttaattg gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc
    12781 aatatgaatg cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat
    12841 gggcatacaa cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct
    12901 gcggggggat atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat
    12961 cggattcagg cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg
    13021 tacaacggaa atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc
    13081 actatgggaa acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca
    13141 agcttgcgtg cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc
    13201 gaaagcgatg gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc
    13261 gctaagggta cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt
    13321 tggcaggatc aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga
    13381 acttacacat tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat
    13441 atcaaaaatt atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca
    13501 aaaattacaa tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc
    13561 aaggcttcag gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact
    13621 gcggatcgtt tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc
    13681 aggaatcgaa cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt
    13741 ttaccaaaac gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt
    13801 caacgatttc gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt
    13861 tgtttaagaa agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga
    13921 tagaaaaaag ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg
    13981 aaacaaatta acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa
    14041 atcaagcatt ttagctattg aagaatgttg atttataggg attccccctc catattatgt
    14101 tggctcttta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
    14161 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
    14221 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
    14281 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
    14341 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
    14401 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
    14461 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
    14521 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
    14581 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
    14641 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
    14701 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
    14761 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
    14821 tgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
    14881 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
    14941 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
    15001 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
    15061 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
    15121 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
    15181 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
    15241 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
    15301 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
    15361 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
    15421 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
    15481 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
    15541 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
    15601 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
    15661 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
    15721 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
    15781 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
    15841 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
    15901 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
    15961 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
    16021 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
    16081 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
    16141 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
    16201 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
    16261 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
    16321 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
    16381 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
    16441 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
    16501 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
    16561 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
    16621 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
    16681 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
    16741 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
    16801 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
    16861 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
    16921 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
    16981 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
    17041 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
    17101 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
    17161 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
    17221 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
    17281 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
    17341 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
    17401 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
    17461 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
    17521 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
    17581 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
    17641 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
    17701 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
    17761 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
    17821 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
    17881 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
    17941 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
    18001 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
    18061 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
    18121 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
    18181 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
    18241 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
    18301 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
    18361 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
    18421 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
    18481 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
    18541 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
    18601 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
    18661 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
    18721 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
    18781 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
    18841 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
    18901 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
    18961 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
    19021 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
    19081 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
    19141 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
    19201 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
    19261 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
    19321 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
    19381 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
    19441 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
    19501 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
    19561 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
    19621 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
    19681 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
    19741 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
    19801 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
    19861 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
    19921 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
    19981 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
    20041 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
    20101 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
    20161 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
    20221 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
    20281 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    20341 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    20401 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    20461 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    20521 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    20581 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    20641 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    20701 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    20761 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    20821 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    20881 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    20941 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    21001 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    21061 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    21121 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    21181 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    21241 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    21301 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    21361 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    21421 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    21481 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    21541 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    21601 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    21661 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    21721 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    21781 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    21841 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    21901 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    21961 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    22021 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    22081 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    22141 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    22201 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    22261 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    22321 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    22381 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    22441 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    22501 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    22561 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    22621 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    22681 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    22741 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    22801 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    22861 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    22921 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    22981 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    23041 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    23101 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    23161 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    23221 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    23281 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    23341 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    23401 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    23461 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    23521 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    23581 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    23641 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    23701 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    23761 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    23821 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    23881 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    23941 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    24001 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    24061 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    24121 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    24181 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    24241 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    24301 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    24361 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    24421 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    24481 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    24541 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    24601 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    24661 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    24721 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    24781 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    24841 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    24901 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    24961 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    25021 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    25081 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    25141 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    25201 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    25261 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    25321 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    25381 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    25441 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    25501 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    25561 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    25621 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    25681 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    25741 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    25801 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    25861 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    25921 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    25981 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    26041 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    26101 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    26161 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    26221 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    26281 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    26341 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    26401 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    26461 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    26521 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    26581 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    26641 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    26701 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    26761 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    26821 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    26881 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    26941 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    27001 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    27061 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    27121 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    27181 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    27241 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    27301 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    27361 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    27421 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    27481 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    27541 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    27601 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    27661 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    27721 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    27781 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    27841 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    27901 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    27961 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    28021 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    28081 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    28141 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    28201 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    28261 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    28321 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    28381 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    28441 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    28501 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    28561 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    28621 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    28681 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    28741 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    28801 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    28861 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    28921 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    28981 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    29041 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    29101 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    29161 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    29221 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    29281 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    29341 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
    29401 agaagttggc aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca
    29461 gtcccttttt tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt
    29521 taattcataa acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga
    29581 gataccttcg atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac
    29641 agttttgttg tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc
    29701 taatgtcact ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg
    29761 acggtcattt gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta
    29821 agaacgacgc cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt
    29881 caaaccttgt aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac
    29941 ccaaagttga tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac
    30001 agaagtgtaa gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt
    30061 tgcttcttcg attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa
    30121 tgcattccat tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc
    30181 ttttaagata ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg
    30241 aagagatttc catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa
    30301 aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat
    30361 ttctttttca ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga
    30421 agaaataaat tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt
    30481 ttttgcatcg atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc
    30541 aactaatgta cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg
    30601 gaaatagatt tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag
    30661 cccgcgatct ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg
    30721 atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa
    30781 atgaacagca gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca
    30841 accaaagtca gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg
    30901 tgcataacca ataattttca tggtgattcc tttctagcag tgattttcta actggatata
    30961 acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg
    31021 tcttttttta ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat
    31081 ctcaacgcct ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg
    31141 ttctactacc ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac
    31201 ttctagatgc agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc
    31261 gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga
    31321 gccataaggg atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga
    31381 agaatcaacg atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa
    31441 agcagcgccg ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat
    31501 tgtcaaatcg actacgggga cttcatcaat actctttaac ggtattccta atttttccgc
    31561 ttgttctttt gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat
    31621 acggcgacca atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat
    31681 catcccgtcc tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt
    31741 tgcgtccacc tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac
    31801 ggtaaaaaga taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc
    31861 ttgttgacat gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct
    31921 gtgaagaaac gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt
    31981 tattttttat cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga
    32041 aggaggaaac aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag
    32101 gtggaaaaat ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa
    32161 actaacgtaa actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct
    32221 aaaaaaccga actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa
    32281 gttactgctt atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta
    32341 cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta
    32401 gatactgctg gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct
    32461 aaagcttaat tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac
    32521 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
    32581 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
    32641 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
    32701 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
    32761 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
    32821 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
    32881 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
    32941 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
    33001 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
    33061 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
    33121 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
    33181 gtaaaacgac tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg
    33241 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
    33301 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
    33361 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
    33421 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
    33481 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
    33541 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
    33601 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
    33661 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
    33721 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
    33781 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
    33841 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
    33901 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
    33961 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
    34021 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
    34081 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
    34141 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
    34201 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
    34261 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
    34321 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
    34381 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg
    34441 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
    34501 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
    34561 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
    34621 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
    34681 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
    34741 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
    34801 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
    34861 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
    34921 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
    34981 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
    35041 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
    35101 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
    35161 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt gaaaccggat
    35221 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
    35281 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
    35341 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
    35401 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
    35461 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
    35521 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
    35581 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
    35641 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
    35701 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
    35761 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
    35821 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
    35881 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
    35941 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
    36001 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
    36061 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
    36121 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
    36181 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
    36241 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
    36301 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
    36361 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
    36421 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
    36481 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
    36541 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
    36601 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
    36661 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt
    36721 attttctaaa tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg
    36781 tgagtttata tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta
    36841 atcagaatga tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca
    36901 cgaacgagcg tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga
    36961 gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt
    37021 ttagatcaat cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct
    37081 ggtccaattc cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa
    37141 tacaaagatt cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg
    37201 aacccaacac caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac
    37261 atcgaaatca aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga
    37321 atcttaggga aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca
    37381 gtaactgttg tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat
    37441 ggctacgaag ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct
    37501 gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa
    37561 aatgttgagc taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca
    37621 ggagacatta ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa
    37681 cgccacggac aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg
    37741 tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg
    37801 ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat
    37861 gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca
    37921 gctgtgaaag ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca
    37981 ttattcaaac aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga
    38041 attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga
    38101 caaggaacac acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg
    38161 cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga
    38221 ggtggcgtag tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt
    38281 cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca
    38341 atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac
    38401 ttatctatcg actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta
    38461 tcagcacgta acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac
    38521 gttgtatcaa atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg
    38581 cttgcataat gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc
    38641 ttgccatgga tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag
    38701 taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg
    38761 tacgtgcgaa attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag
    38821 cgatcgttac attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag
    38881 cactaataga taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc
    38941 tacctcaaat ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac
    39001 acctgaaaaa tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg
    39061 catcactgta cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa
    39121 acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc
    39181 agctaacatc gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa
    39241 aggactagaa gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa
    39301 tgctcttcca ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc
    39361 tggtaaaggt ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga
    39421 aggcaaatac gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg
    39481 ccgtgctaca atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc
    39541 tggacgctct cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa
    39601 tgatcaccca cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc
    39661 tccttggggt caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa
    39721 acttattgtt cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga
    39781 gaggaggttc accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat
    39841 gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg
    39901 ccgttcaaca gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa
    39961 acatgtacca gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc
    40021 tacaagaact tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg
    40081 gaggataaat ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc
    40141 gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag
    40201 atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat
    40261 tgatgtcagc agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag
    40321 taagcgaagc ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag
    40381 gatcagcttc tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat
    40441 aaggagggat aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc
    40501 cgcgactggg atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat
    40561 ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa
    40621 atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt
    40681 attggtaaag gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt
    40741 aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta
    40801 ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa
    40861 gccatccaac gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt
    40921 ctaaacggag cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat
    40981 actttacgtg cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta
    41041 ggagttaaag tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa
    41101 ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa
    41161 aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag
    41221 ctgttgattc acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc
    41281 gttacatgaa acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg
    41341 ccaaagcaat tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac
    41401 cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg
    41461 aagcgttacg tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg
    41521 aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg
    41581 cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca
    41641 ggtcaattag aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa
    41701 actgtgttgc gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa
    41761 ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca
    41821 attaccgttg ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat
    41881 tctaagaaat acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga
    41941 attatggaaa ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa
    42001 gaagcagtta ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa
    42061 aggaggatac acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc
    42121 gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc
    42181 ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt
    42241 gacgtcgtta aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca
    42301 tatattaaat ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga
    42361 acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt
    42421 tcactagcac cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt
    42481 atgtttgtta aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa
    42541 ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc
    42601 gttaaaaaac accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa
    42661 gcgccaattc atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt
    42721 ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta
    42781 gataaataaa agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat
    42841 cttaaagaag taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca
    42901 cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa
    42961 aacttagata aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca
    43021 aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa
    43081 gttacattgc gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta
    43141 ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat
    43201 actttaggta ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa
    43261 gtacgtggta tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag
    43321 ttgttgacac aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct
    43381 aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact
    43441 cgttgcgaac gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt
    43501 tgcttccgcg aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa
    43561 aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag
    43621 attttctaac tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg
    43681 catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg
    43741 ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa
    43801 acggcgaacg tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg
    43861 tcaaagctga cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt
    43921 ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg
    43981 cttacgtatg gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga
    44041 aataaagtcg ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca
    44101 gttaaaggac caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc
    44161 gaaggaaacg aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat
    44221 ggaacaactc gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa
    44281 gcgttagaac taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg
    44341 aacgttggtt actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta
    44401 ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct
    44461 gcaaacattc gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt
    44521 ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca
    44581 tagaagccag cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt
    44641 ttacttgtgc cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa
    44701 taaaaataaa gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa
    44761 gagacatcgt cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt
    44821 ttttcgttct aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct
    44881 agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca
    44941 agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt
    45001 tgaccgtggt ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga
    45061 aaatggacta gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa
    45121 catttggaat tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt
    45181 ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtagga
    45241 tttggtacag gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg
    45301 aagaaaaact tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt
    45361 gcttttagtg gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct
    45421 ggtggaccag ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct
    45481 ttaggctcaa atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg
    45541 aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa
    45601 ggaggacaac aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc
    45661 ctcaaaacca acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg
    45721 ttaagccttc taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg
    45781 ttgaagaaat ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg
    45841 aaacttcatg aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt
    45901 ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt
    45961 tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta
    46021 ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta
    46081 ttgaaccgct ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc
    46141 gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta
    46201 actgtgaaag cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga
    46261 tcaatcgagg tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta
    46321 gatcgaaaat cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta
    46381 ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca
    46441 tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt
    46501 caccatacat tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat
    46561 ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat
    46621 atctgacatt agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat
    46681 acgctcaatt aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga
    46741 ttctaacagc cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta
    46801 tcggaaatgg cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta
    46861 tcagagaaat ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg
    46921 ctatttttgt ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt
    46981 tccaacaagc tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta
    47041 caagcagcta tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta
    47101 gttcgtttat tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg
    47161 aaaactggta caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga
    47221 tttatacgat tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg
    47281 agaaattagc ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta
    47341 aggggactga agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct
    47401 tccttggttt agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat
    47461 caatcggatt aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga
    47521 aacagttaga aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg
    47581 tgttgaggtt tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac
    47641 ctcattttaa tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat
    47701 gcttatggta ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa
    47761 acagctcttg gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa
    47821 gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta
    47881 ttagatggct ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat
    47941 ttgaataaaa aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa
    48001 cgtctagccg gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac
    48061 cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa
    48121 gatgataaac ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca
    48181 atcttggctt attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc
    48241 gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg
    48301 acttgcatta tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc
    48361 gacttctaga gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt
    48421 tccgccattt tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa
    48481 agaagatatg attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa
    48541 agttgaacta gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca
    48601 ttacatccgt atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa
    48661 ccgtggccgt attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt
    48721 atcatgaaag taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt
    48781 aaaggacgcg ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa
    48841 ttggaggtgc tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg
    48901 tagtagtttc acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag
    48961 ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg
    49021 ctatccgtgc agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact
    49081 tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat
    49141 tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg
    49201 ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa
    49261 aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc
    49321 attctacatt caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt
    49381 ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc
    49441 aaatggcagc agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag
    49501 taacagttaa aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag
    49561 gtttagaagt gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc
    49621 caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg
    49681 aacaagacac ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca
    49741 agaatcgcaa aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa
    49801 agagggtatg ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca
    49861 ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa
    49921 ggtgttagag aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg
    49981 tatggccaag aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct
    50041 ggcgatatta tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt
    50101 gtctctgaag gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt
    50161 caagcagatg aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc
    50221 tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa
    50281 gattttgata aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca
    50341 atgagtttgt ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat
    50401 gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg
    50461 gaaatgacga tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca
    50521 ggaatcaata ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt
    50581 aacttaggac gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt
    50641 ttacgtaaag aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa
    50701 ctaggacgca catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta
    50761 atcaacgaac gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa
    50821 aaaatgatca ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc
    50881 gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct
    50941 ttacaaaaac tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca
    51001 cgcattttga agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt
    51061 gtctaaaatc acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt
    51121 ggaagatatt tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag
    51181 taagcactca tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt
    51241 tgaaggcata aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata
    51301 gtgttagtag gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga
    51361 aaagataaat tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt
    51421 tacaattgat aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac
    51481 acttgccaaa acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa
    51541 tcaaatattg gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca
    51601 aaatccagac aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga
    51661 aaatcaaggg attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt
    51721 tagaatggct gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg
    51781 tgttgccatt gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac
    51841 cagtatgcta gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga
    51901 agaaagtcaa ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa
    51961 tcggatacta gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt
    52021 ttctgctggt cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa
    52081 gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat
    52141 ggtggattgg ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca
    52201 ccatttgaac aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact
    52261 gcattagtag ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt
    52321 gttaagccaa caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat
    52381 aataagaatt taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca
    52441 cagctattcg aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc
    52501 agcgaagaag aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa
    52561 agctatttgg aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc
    52621 gctggtgtgc ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt
    52681 gatccccagg gcagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag
    52741 atcactattg tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc
    52801 tatgtgttgg aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac
    52861 attgaatggc taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa
    52921 ttaatggcta aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac
    52981 gctatcgtcc attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta
    53041 tatgcctgga gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt
    53101 ttattttatc ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt
    53161 tttcacatta ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat
    53221 tcgcccattg atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg
    53281 cggagaaact tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg
    53341 cctctttatt ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac
    53401 tacaccgcct ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt
    53461 acggttccca gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac
    53521 attgatggac gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg
    53581 tgaaggaaac ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt
    53641 cagcagcttc aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg
    53701 tggggaagga agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat
    53761 cattgctttt gtattattaa ctgtgctact gatcttttta agaggataaa cgttcagtgg
    53821 gccgctatct attcttttct tgctgatctg tttgcaagaa aggatgcaag cagtgtatga
    53881 caagaaatat tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa
    53941 gaaagaacga aaaaatttta tagaaaaatc attattccag ataacctttt aggaacaaag
    54001 gagagaaaaa catggtacgt tacaaagcaa tcattgcgta cgacggaaca aattttaatg
    54061 gattccaaaa acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa
    54121 aaatggcaaa tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac
    54181 atgcgatcgg gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc
    54241 gtttcgcgct agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt
    54301 cagaagactt ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata
    54361 tagggaaacc acgcagtcct tttcgacgcc attatgcaag ttattttcct tacccacttg
    54421 atttggaaaa aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt
    54481 tttgtgcatc aggaagctcg atagaggata aaatacgtac gatctacgag gctaagatgg
    54541 aggtaaatga aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata
    54601 agatgatacg gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag
    54661 atagtatccc agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacacc
    54721 ctgaaggact ttatttgtat gaagtgaaat atgaataaga ggttatgata gaagtggtca
    54781 gcttcaagaa ataagaaggt atttacgaaa attacttctc aaaattttgc aaaatttcgg
    54841 cttattttcg aagaagctac ttctggaaca ccgtttaagg aagaggtcgt gaaatcagcg
    54901 ttttgacctg agtattagag aaacaaacgg gaaaatagct cctcatattt tgccgtttgt
    54961 tgagctaata ccgaaggtca gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt
    55021 catttaactc tgagtcacaa agagcaattt caaaaaacag cttctcatct ttgatgaata
    55081 tcgtgacttg tggacgaggc gttgataagt gaatcatctt tttgttacct attttttctg
    55141 taaccaaaaa acgatggaac gttgttttga ttgcccttgg tgcggacagt ctgaacaggt
    55201 tccatcgttt ctttattttc tacgtcttct tctgtgtgtg tttcgtggtt gttcttctat
    55261 tgctggatca atcgtgccat aaaaggggcg attgcggtca aggtaccaga cataaagaat
    55321 aaacaatgca atacatagga caaaaaggat tgcacctatc gtaaatcctt caggcaagta
    55381 actgaaagtg acagtatgtt tacctgctgg aacttttatc atcaagaatg cgtctttgaa
    55441 attgctgacc ttgactggtt tcccatcaat tttagctttc catcctttgt cataaggaat
    55501 agttgtcacc aacatggaat cttcagcggt atttacagtc ccagaagctt ttctgccagt
    55561 ggcttccatt tcgacacctt tttctttgat ctgattcatt gcggcttgat aggctacaac
    55621 atctagacca acgactttag gctccatgaa acttatttct ttcgtcccat aaaaacttgc
    55681 agtaaatgtc actgttgtct cttgaggata atagccaaga tcatagtatt gaccagaaat
    55741 gttgatttgc gtttttcgcg atgtaccatt gacagtgatc gtcgcactag aactttctaa
    55801 ttgcgcaaaa ttggttggga acaaacttaa gtaagcttgt gtatttgcag gcacatggac
    55861 ttcccaagtg agctcttttg gctggttatc tgctgtttct gagtaagtga cgccagtcgt
    55921 tgtctttgtg atcgtcgtat tcaactgatt cttcaaagta ggtgtataga atgtaaaata
    55981 ctgttcattt agaccagaca atgtattgat caagtttgtc tgactggtca aattatctgt
    56041 cactggctgc tcaacatcat taacggacaa cggtgcagta aatgctagtg gcaatgcatt
    56101 gacattctga tacagtgtat agtcgccgct ttgaccagct tggcggaagc catatttact
    56161 gaacgtgctt tcgtttttag aaatgttata tttgattcct gtaaacccat ccatcagcaa
    56221 agtattattg ttataacgga tattcaaatt tgtccctctt gaacggaaac ctaatttatc
    56281 caggtaagag gaagaattac gatttcttat agaagaaaac aaactgatac cactgtaacc
    56341 atagttgatc gaatcgtttg cagatactgg atccagattt tccaagcggt aaaacgagtc
    56401 ggaatctttt tttgtttcat ttacaagagc tttcaagtca ggataaggat cagtatataa
    56461 actgcgggaa gcataattcc aatcatcaag aatgccatgg atcatactgt tcgtattgac
    56521 cagtgcttca gcagacatga tcaataataa caatatactc agatgattga atgaaagttt
    56581 tttatgctga taaaatccca cagcaaaagc ataaatccct aagaaaacca ctgtcaatac
    56641 aaaggaagtc atcgtcacat aggtgtagct tccttcaggt ttgatgcccc atgccaccgc
    56701 aaagagagca gctacgacca aaatgattcc taacaacagc ccacgatcgt cttgttctat
    56761 cttttcccat ccttttcctg caagcaggag cactaagaaa gagaacagat aggcataacg
    56821 gaataaaaac atattcggtg catgcatacc atgccagaac aaattcaatg gaacaagata
    56881 aaaacttgcg ataagtatgg cgaaaagact gccgtacaaa aatttatttt tcaacggtac
    56941 tttccgactg gcaaaataaa acagacagag gactaatggt agcagacctg cgtaaacgaa
    57001 cggaatcgat ccgtattttg tcgtgtcata aacaccaatc atatttttca tgattagatc
    57061 cagataagaa gtcgcttctg ttttcaacgt agtgatctga ctcagtgcct cgccatttga
    57121 ccgaagatcc agcactgctg gcaatacaat gatcattgaa gcgccgcctg ccagtaaaga
    57181 agtgatacca taaggaacaa tcgttttttt atattctgac cagtttgtca atagacgggc
    57241 gataaaatat aagaaagaaa atacgccaat cataaaaccc atataaaaac tggataaaaa
    57301 caagagtaaa taactgataa ataaaagttt cggccgttta agatccataa cacgatgaat
    57361 ccccaaaatc actaatggaa gataaaaaaa tgcatccagc cacatgatga tctccgaatg
    57421 tgcagtgata aatgagatca atgcgtaact gacgctaagt gcaacatgcc cccagcgagg
    57481 aatcttgaac gtacgtgcaa gaaaccaaaa agccaatccg gcacagccga ccttaagcaa
    57541 agtcagaaca tacagtgcat ctggcatcat ctggtttgga aaaaagaaca ccagtggcgt
    57601 gaagatcccg cctagatagt aagaaattaa agataaataa ttcaatccta gagaagcatt
    57661 ccatgtataa aaaatacttt gctttccaag aagaacattt cggaagctgg catgaaaatt
    57721 agaaaactgt gaaaaggcat cacttgccaa aatacttctg ctgcttccag gatagattcc
    57781 gatacttaga taaacaagtg ctaaaatcaa aaacggaatc aggaaactag ctgctgcgta
    57841 gcgtccgttt tctctaaaga atgactttaa attttttttc atatttattt tccacca
//
LOCUS       DAZUKD010000004        47470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000004 DAZUKD010000000
VERSION     DAZUKD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.004"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8N9_000193"
     CDS             complement(55..789)
                     /locus_tag="ACG8N9_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4895830.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG8N9_000194"
     CDS             1116..1526
                     /locus_tag="ACG8N9_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4895831.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG8N9_000195"
     CDS             1519..2220
                     /locus_tag="ACG8N9_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4895832.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8N9_000196"
     CDS             2220..3806
                     /locus_tag="ACG8N9_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4895833.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8N9_000197"
     CDS             3825..4046
                     /locus_tag="ACG8N9_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4895834.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8N9_000198"
     CDS             4043..4804
                     /locus_tag="ACG8N9_000198"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4895835.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8N9_000199"
     CDS             5184..5693
                     /locus_tag="ACG8N9_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4895836.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8N9_000200"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8N9_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4895837.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8N9_000201"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8N9_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4895838.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8N9_000202"
     CDS             7320..8171
                     /locus_tag="ACG8N9_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4895839.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8N9_000203"
     CDS             8199..8834
                     /locus_tag="ACG8N9_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4895840.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8N9_000204"
     CDS             8854..9627
                     /locus_tag="ACG8N9_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4895841.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8N9_000205"
     CDS             10035..10832
                     /locus_tag="ACG8N9_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4895842.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8N9_000206"
     CDS             10810..11223
                     /locus_tag="ACG8N9_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4895843.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8N9_000207"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8N9_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4895844.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8N9_000208"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8N9_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4895845.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8N9_000209"
     CDS             16000..16560
                     /locus_tag="ACG8N9_000209"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4895846.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8N9_000210"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8N9_000210"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4895847.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8N9_000211"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8N9_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4895848.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8N9_000212"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8N9_000212"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4895849.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8N9_000213"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8N9_000213"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4895850.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8N9_000214"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8N9_000214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4895851.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8N9_000215"
     CDS             21907..22485
                     /locus_tag="ACG8N9_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4895852.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8N9_000216"
     CDS             22537..23019
                     /locus_tag="ACG8N9_000216"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4895853.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8N9_000217"
     CDS             23136..23510
                     /locus_tag="ACG8N9_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895854.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="ACG8N9_000218"
     CDS             complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="ACG8N9_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4895855.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25687..26277
                     /locus_tag="ACG8N9_000219"
     CDS             25687..26277
                     /locus_tag="ACG8N9_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4895856.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26405..27754)
                     /locus_tag="ACG8N9_000220"
     CDS             complement(26405..27754)
                     /locus_tag="ACG8N9_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4895857.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27922..28662)
                     /locus_tag="ACG8N9_000221"
     CDS             complement(27922..28662)
                     /locus_tag="ACG8N9_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4895858.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28675..30267)
                     /locus_tag="ACG8N9_000222"
     CDS             complement(28675..30267)
                     /locus_tag="ACG8N9_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4895859.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     regulatory      complement(30405..30579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(30835..31131)
                     /locus_tag="ACG8N9_000223"
     CDS             complement(30835..31131)
                     /locus_tag="ACG8N9_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895860.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31307..32032
                     /locus_tag="ACG8N9_000224"
     CDS             31307..32032
                     /locus_tag="ACG8N9_000224"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4895861.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32025..32867
                     /locus_tag="ACG8N9_000225"
     CDS             32025..32867
                     /locus_tag="ACG8N9_000225"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4895862.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32870..33391
                     /locus_tag="ACG8N9_000226"
     CDS             32870..33391
                     /locus_tag="ACG8N9_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4895863.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33644..34207)
                     /gene="def"
                     /locus_tag="ACG8N9_000227"
     CDS             complement(33644..34207)
                     /gene="def"
                     /locus_tag="ACG8N9_000227"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4895864.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34458..34727
                     /gene="rpsO"
                     /locus_tag="ACG8N9_000228"
     CDS             34458..34727
                     /gene="rpsO"
                     /locus_tag="ACG8N9_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4895865.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34915..37041
                     /gene="pnp"
                     /locus_tag="ACG8N9_000229"
     CDS             34915..37041
                     /gene="pnp"
                     /locus_tag="ACG8N9_000229"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4895866.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37551..37817
                     /locus_tag="ACG8N9_000230"
     CDS             37551..37817
                     /locus_tag="ACG8N9_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4895867.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37961..39952
                     /locus_tag="ACG8N9_000231"
     CDS             37961..39952
                     /locus_tag="ACG8N9_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4895868.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40306..41607
                     /locus_tag="ACG8N9_000232"
     CDS             40306..41607
                     /locus_tag="ACG8N9_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4895869.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41737..43107)
                     /locus_tag="ACG8N9_000233"
     CDS             complement(41737..43107)
                     /locus_tag="ACG8N9_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4895870.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43289..43612)
                     /locus_tag="ACG8N9_000234"
     CDS             complement(43289..43612)
                     /locus_tag="ACG8N9_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4895871.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43937..44545)
                     /locus_tag="ACG8N9_000235"
     CDS             complement(43937..44545)
                     /locus_tag="ACG8N9_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4895872.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44875..45591
                     /locus_tag="ACG8N9_000236"
     CDS             44875..45591
                     /locus_tag="ACG8N9_000236"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4895873.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45604..46299
                     /locus_tag="ACG8N9_000237"
     CDS             45604..46299
                     /locus_tag="ACG8N9_000237"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4895874.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46383..47012
                     /gene="udk"
                     /locus_tag="ACG8N9_000238"
     CDS             46383..47012
                     /gene="udk"
                     /locus_tag="ACG8N9_000238"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4895875.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15391 a         8788 c         9878 g        13413 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat
    23881 aaaagaaatg ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt
    23941 atatggagct actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa
    24001 taaacggtgt tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga
    24061 agccattttc aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc
    24121 aataaacagt gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc
    24181 tcttttatca atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa
    24241 aaccgataac tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt
    24301 ccgcaacata taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt
    24361 tcaacccatc aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg
    24421 tacgctgctt aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat
    24481 agatgaacat caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg
    24541 ctaaacaagg aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca
    24601 aatctgtgaa tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta
    24661 atataacgat aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct
    24721 gagccagcgt gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta
    24781 aataagcgag caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga
    24841 tcgtgtcttt agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa
    24901 tgatgatccc aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc
    24961 ctgtgaaaaa agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta
    25021 gtggattcaa aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca
    25081 ggaactttcc aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca
    25141 caaaaaatac aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg
    25201 gtgctaaacc aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga
    25261 taactaagta aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta
    25321 attcatatac acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac
    25381 cgataccggt aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac
    25441 ccaaatgaac gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc
    25501 ctataaaaag atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt
    25561 ttttaaaagt aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa
    25621 aacgacggtt atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga
    25681 atcatcgtga caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg
    25741 agagttcttg tattagagat caagatccct aatacatcta gctttcattc tttatgggtc
    25801 atccgaaata cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg
    25861 aaccatcatc gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat
    25921 atctttttgc tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac
    25981 cctgaagctt tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct
    26041 tttggtatct tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga
    26101 gttttgtcaa aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc
    26161 ggtgtctttt ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg
    26221 attttttcct ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa
    26281 agaggctggg acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa
    26341 gaagcaattt tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac
    26401 tttcttactt cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca
    26461 caggtgttag taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa
    26521 aggccatgta aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat
    26581 gagctttttt gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc
    26641 agataaacaa gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca
    26701 tcacgtaatt caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc
    26761 ctggcacatg atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta
    26821 aagaacgaag catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac
    26881 ttaatacgac taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt
    26941 ctacaaatgg actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa
    27001 aaagagatcc taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca
    27061 acactttttc aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa
    27121 aagcaaaggt taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc
    27181 cgccatgaga ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga
    27241 ggatcatata agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa
    27301 accaaaactc taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac
    27361 ctaatgcaat cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca
    27421 ttccgatggc tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc
    27481 cagtgatgaa agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt
    27541 tattggtgtt tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc
    27601 cagtaataag ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac
    27661 ctaaaaacaa ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt
    27721 tcagtccacg ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt
    27781 ttaattactt ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc
    27841 agaaatttac cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt
    27901 tgtcccaatc agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg
    27961 ttcttctttc ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt
    28021 atccataaaa atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat
    28081 aagcattgtc aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat
    28141 ttcagggtct aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa
    28201 tgcccgagca attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc
    28261 gatataacga tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc
    28321 tttatctcgt ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt
    28381 gttgaacagg ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt
    28441 atatttagga gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc
    28501 cagtaaattg atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt
    28561 cacaacttca cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa
    28621 gctttttctt agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc
    28681 ctgcaaattt gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt
    28741 cttttctacc gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc
    28801 gacgatcgta aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa
    28861 gaacaaatca gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa
    28921 ctcatttcct gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat
    28981 cgtttggcca tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc
    29041 aaaaatcccc ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa
    29101 caaagcggca atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat
    29161 gaccattgct tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag
    29221 tgcatttccg attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg
    29281 gaagacacca atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa
    29341 caaggtaagt ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa
    29401 atcagaaatg atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc
    29461 ttggtccata attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt
    29521 ttggatatcc gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt
    29581 ctggaagcca tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc
    29641 cgttacacct tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt
    29701 tctcattgct gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat
    29761 cacagtatag tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa
    29821 actttttgct tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta
    29881 ataaggatcg gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat
    29941 gatcgcgtcg atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac
    30001 gatgactaat tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc
    30061 gccggcgtaa ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga
    30121 ccagttgaag ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt
    30181 ttcatctgca tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac
    30241 ggcaactgaa accatatttt ttttcatttt tttctccctt tctgattata tgatacacga
    30301 gcggtctaga tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata
    30361 gatataaaaa ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa
    30421 cttagcatca ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca
    30481 agcaaaaaag tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac
    30541 tccacagggt taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag
    30601 ttatttatac aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt
    30661 ttttgaaaaa atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat
    30721 gtgaatcatc tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca
    30781 ataaaaaaag aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc
    30841 tgccgctttc tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat
    30901 ggctaatatt agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata
    30961 tacggctaat gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca
    31021 aagatttcca ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa
    31081 ccaggcgata caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca
    31141 cctcactttt atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg
    31201 ataaattgtt caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat
    31261 aaatttgttc gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac
    31321 agtagacgaa gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc
    31381 aggggtatcc acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg
    31441 gatggagcag ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt
    31501 ctatcaattt gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga
    31561 aatggcacat ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt
    31621 gcacgcaaag gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca
    31681 ttgtcgaaaa tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca
    31741 agcatgcgga ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg
    31801 agtcatggag caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac
    31861 gagttttcaa gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat
    31921 cttagtaatc aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga
    31981 cggagcggtt gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa
    32041 ccgaaaaaga aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg
    32101 ccgaccgtat gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac
    32161 tgcgatccca attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc
    32221 cagttatcaa ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt
    32281 tcaattgtac atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc
    32341 cgaatgtcca gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc
    32401 tggagtacgc aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg
    32461 tcacgccggg tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca
    32521 aatcttttcc cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag
    32581 aggaagaact aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc
    32641 gagagttgat tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga
    32701 aaaaagaaga aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag
    32761 ttgttagtct gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt
    32821 tgatgaaaca ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg
    32881 agagacacgg cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc
    32941 tagcaagttt gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt
    33001 actacgagct gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga
    33061 agaaaccaat actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga
    33121 agaagaactg agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca
    33181 tcccctttac ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc
    33241 ctttatgaga cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat
    33301 ccatctgcat acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact
    33361 agctgaagca ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa
    33421 actattctat catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa
    33481 gatgcgtatc caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt
    33541 tcgattagtg gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat
    33601 aaagctttct tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata
    33661 cgccttcttt tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt
    33721 tgatatggtc gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt
    33781 gtttttcacc attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc
    33841 ctggtacttc tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa
    33901 cagaatggct caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct
    33961 ctgggtcagg acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag
    34021 cagctaagcc aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt
    34081 tttttaagaa ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag
    34141 aaacttcttt tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg
    34201 taatcatgga atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta
    34261 tagtttacca caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa
    34321 tgattaacta cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca
    34381 catgacctgc cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat
    34441 cgaaagaggt gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat
    34501 acgctcgcca tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag
    34561 aaatcaacca cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg
    34621 gcctaatgaa aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg
    34681 ttcaacgtta ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa
    34741 gagtgggatt cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt
    34801 gatcaagcta gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc
    34861 agttttcatt tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca
    34921 gaaaaacaag tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag
    34981 ctagcaaaac aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt
    35041 gcagctgtgg cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat
    35101 gaagaaaaaa tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt
    35161 ccaagtacgg aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt
    35221 gcagaaggct tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac
    35281 tgctcgccag ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc
    35341 ccatttgacg gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg
    35401 aatccaacag tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa
    35461 caagccatca atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt
    35521 gcattgctgt ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt
    35581 caagcagtag ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa
    35641 aaagaaatct ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa
    35701 cttgctcgcg aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag
    35761 aaatttgcag gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa
    35821 gatcttgaaa aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg
    35881 cgtaaattgg atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat
    35941 ggatcaggtt tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct
    36001 ctaggagagc accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac
    36061 cactacaatt tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt
    36121 cgggaaatcg gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa
    36181 gaagaatttc cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct
    36241 tcccaagcaa gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa
    36301 gcgccagtcg ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta
    36361 actgatatcc aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca
    36421 aaagatggta tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc
    36481 ctaactgaag cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact
    36541 tcaacgattg ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc
    36601 cagatcgaac ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga
    36661 atcatcgatg aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct
    36721 tcagctgatg cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa
    36781 gtcaaagttg gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc
    36841 gtcaacttga tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt
    36901 gtcaataaag tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa
    36961 atcgataaac aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca
    37021 acgaaaaatg aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt
    37081 gacaggggga caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc
    37141 ttttttttcg aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt
    37201 taaaaacatt aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt
    37261 tttacaacct ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa
    37321 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcaggt caaaccgctg
    37381 atttcacaac ctctgattca cggtgttcca gaagcagctt ctacggaaat aagctgaaca
    37441 cttttgttac aacttctttt tttctggagt ttcttggcgc atatttttgg agtccttctg
    37501 aataaagcgt aaaataaaag tgagaaatat ggataaagga gggaaaggag atgctgaatt
    37561 ttttatggtc gttatttatc ggcggtatat taggtttgat agctggcgca attttaggaa
    37621 aggaagtccc gggaggcgta tctggaaata tcattatcgg ttttttaggt agttggctcg
    37681 gcgaattttt actaggacca ctgggaccgg aactcggcgg tttttatttg ataccagcat
    37741 tattgggtgc aattttttgc cttgctcttt attcatttgt ggcaagacgt atcagagaac
    37801 gtccaaagaa agaataattt tattcaaata agttgacact tcagtgtgtt acttggataa
    37861 ttagaagcaa tttaggaaag cgggtgtcat ggataagaat atcatctatc attcaagctt
    37921 acctaaagga tatccatata acaagaaatg agggaaaaac gtggcaacaa cgcacaatac
    37981 caaaaagata tcaatgactg gcctgcttgt agcgatggga gtcgtctacg gagacatcgg
    38041 gacaagtccg ctttacgtta tgaaatcgat tgttgaagat aatggcggat tgtcaggaat
    38101 caatcctgat tttgttatcg gatcagtctc attgattttt tggacattga ctctactgac
    38161 aacaatcaaa tatgtcgtga ttgcattgaa tgcagataac catggtgaag gtggaatttt
    38221 ctcactctac acacttgtcc gaaaaggtgg gaaatatttg atcattcctg caatgatcgg
    38281 aggagctgcc ttactagcag atggggtatt gacccctgct gtgacggtaa cgactgcgat
    38341 tgaaggcttg cgaggaattc cgcagttttt tgaccggttc ggtaataacc agaacgtgat
    38401 cgtcattatc actttagtta ttatttttgt tttattcatg gtccaacgct ttggtacaga
    38461 tattgtcgga aaagcattcg gaccaatcat gttcgcttgg tttacttttt taggcgtaat
    38521 gggattaatg aacttcagtc aagactggac agttatccgt gcactgaatc cttattatgc
    38581 tatccagctg ctttttagtg cagataacaa attaggcttg ttcattcttg gaaatatttt
    38641 cctggctacg acaggtgcag aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa
    38701 tattcgtctg agctggcctt acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc
    38761 tgcgtggttg ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga acctgaatcc
    38821 attcttccaa atgatgcctc aaagttggat cgtgatcggc gttgcttttg caacagtcgc
    38881 tgcagtcatt gcttcgcaag cattgattac aggttcgttt acgctagttt cagaagcaat
    38941 caagttgaaa ctgctgcctc ggttaaagat catgtatcct gggaacagta tcgggcaaat
    39001 gtatatccca gcagtcaatc tgattttatg gatcgcctgt tctctagtgg ttgtcacatt
    39061 ccgaacgtct catcatatgg aagctgctta cggtctatcg atcactgtga cgatgttgat
    39121 gacaacagca ctgctttact tttatcttat ccaaaatggg cattctcgtt ggcttgcata
    39181 tttagttacc tttttcttcg gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa
    39241 attcttccga ggcggttttg tagccgttct gattggtttg atcatcttag tcgtgatgtt
    39301 tatttgggaa caagggaaca ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga
    39361 ttatatcccg cagcttagag aattacgtga tgatacttct ttaccaatct atcagacaaa
    39421 tgtggtcttc ttggttcctg atatggctga agataaagtt ggccgacagt tcatctattc
    39481 gattttagat aaacggccta aaagagcacg agtctattgg tttgttcatg tggaagtgac
    39541 ggatgagcca tatacgaaag aatatcaagt agatatgatg gacactgatt ttattgtcca
    39601 agtgaatctg ttcttaggtt tcagggtcca acaggaaatc aacgtatatg ttcgccaagt
    39661 catccatgat ttgatgaaac aaggccgttt gccaaaacag ccacaaagat actctctgac
    39721 accagggcga gaagttggcg atttccaatt tgtcctgatt gatgaagtag tttccaatgt
    39781 gacaactcta ggaaaatggc aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat
    39841 cgctacctcg ccagaaacat ggtttgggct agagtacagc gaagtaaaac atgaaactgt
    39901 tccgcttatc attggaaata ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg
    39961 ctttgacctg aactaatgtc gaatgtcagc tacttgaaca ccgtttatca gaggtcatga
    40021 aaaagctgta ctgcaacaag cagtaagaac gagcagataa aaacagcttc tcatgttttt
    40081 catctgtttg ggcttaatgc cgtagatgca agcttttgaa taccgttttt atcagaggtc
    40141 atgaaaaagc tgtttagcaa caagtaacgc aatgattttg aagtgataag gagatgtggc
    40201 tgaaaaatgc cgcatctcct tttttattcc taaatatgaa tacgcattat tttcaatctt
    40261 tttgtagaaa aagggtacaa tagaaaaaga gaaaggcggg agaatatggt tatcaatttc
    40321 attattttat ttttgatgat tgcaattact tcattttttg tagcaagtga atttgcttta
    40381 gtgaaaatca gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag
    40441 ctagctcttc atgttacaca ccatttagat aactatttat cagctagtca attaggaatt
    40501 acgctaaccg gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa
    40561 ccagtgatcg gtaaactacc ttttagttcg gctattagca gtacaatctc tgtggcgctg
    40621 ggatttattt tagttacgta tatcgatgtg gttattggcg aactgcttcc taagagctat
    40681 agtatcgtca atacggagaa ggtcgtcttg ttcgtagtta aaccacttca ttatttttat
    40741 aaagtaatgt ttccttttat ttgggtattg aatcattcag ctgcagggct agggaagcta
    40801 ttaggtgtgc gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga gctgacatta
    40861 gtcgcattga attcttatga aaaaggagag ttgaccaaag aagaatatta ttatttggaa
    40921 aacgtgtttg aatttgatga taccttggca aaagatatac aagtagaccg tacgtccatg
    40981 caagtattcg aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga
    41041 catactcgct atccggttat cttagaatca aaagacaatg ttttaggtta tgtcaccttg
    41101 ccagatttga tcaagcaatc ttataaagat gaccagctga cagtagaaca gttgattgaa
    41161 gagccgatcg taacaactga aacgatccct ataaaaaaat tattgacgat catgcgaaaa
    41221 aagggtaagc atattgctat cttaaaagat gaatacggag ggacaagcgg cttagtgacg
    41281 attgaagata ttttagaaga aatcgtcggg gaaatccgag atgagacaga tttggacgaa
    41341 gcattgatag cggaacagtc agatggctct tatatcatct ccggcaaact aacattagat
    41401 gatttccagc gttattttca tgtagagatt ccagagtttg aagaaacgaa ttttactaca
    41461 cttgcgggct ttgcttctag ccgatataaa gaaataaaag caggaacaat cattgagatt
    41521 gcatcattcc gtttcacggt attagaatac cagcatgcac atatcgatta tttcaaagta
    41581 gagtccacgg aaagaaaaac agaataaaag aaaaaaggct gtgacatttt ttgtcacagc
    41641 ctttttgtta tctaaaaaac ctgttttcgg taaacgaaga agatattttc aataaaagtt
    41701 ttcattttta gccgcaacga tttcttttaa tcaaggctat cttgcttctg catgttctaa
    41761 tgtgaaatgt tcctcttcac ccatgtgttt caattgtttc atcgaacgca tataagagta
    41821 aagcaacacg gcaacagaac cgatccaagc taaaggagaa gctgctgctg ctcctgggta
    41881 tccccacatt cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt ccatcatacc
    41941 ggcgatcgta gggatcttgc tttgtccaag cccttgaagt gtgtagcgta aaatgaacag
    42001 gactgctaag atccagtaaa gagacccatt gatattgaag taggtttgtg ctaattcaaa
    42061 tacacgtgtt tctgatgggt taacaaacaa ttgaacaaag aaatgaccga agaaaatgac
    42121 gatagcacct gctaagagac taaatccgac actcatcagt aaagtttgac gtaccccttg
    42181 caagattcgg ccatattctt tcgcaccata gttttgtgca gtgaaagttg ccattgcaat
    42241 accaaatgac atcatcggct gatttgcaaa ttggtcgata cgaccagcag cggcttgagc
    42301 agctacgaca tctgtcccta gactatttag tgctgcttgt aacacgatag caccaacagc
    42361 gatgatcgaa gattgaaacg ccattggcaa agcagcattt aggtgtacag caaactcttc
    42421 tttttcaaat ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata
    42481 cactagacac agtacgctgg aagaaacttg tgcgattact gtagcaatag ctgcaccttc
    42541 cacacccatg ccaaaataga taataaagat caaatcaagt acgacgttga tgatgaccgc
    42601 aatgatcaga aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag
    42661 caaattgaaa ctgactgatg caaagatccc taataaaata attgaaataa atgtttgtgc
    42721 ttgatcaagt atttcaggag gagtttgcat caattgaagc atcggacgaa taaacaggag
    42781 acttaggaca gttaaaatca cagtgacgac aagactaatg acaacactag cagcaaaact
    42841 tttcttcaga ccccgataat cttttgcacc gtaacgttgt gcggtaataa tggctaaacc
    42901 agctgttaaa ccttgggcaa agccaatgat caaaaatgtc aggcttccag ttgcaccaac
    42961 cgctgccagc gcatttttac ctagcgtctg acccacgatg atcatatcga tcatattgta
    43021 gaactgctga aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc
    43081 ggggcttcct tttgttaaat cgcgcatctt ttaaactcct tatatcttta aaatttagtt
    43141 agtagtatat agcgtttatc ctgagaatac caattagaaa tcaatgataa tttatgaaac
    43201 tccataaaaa gaagccaaga attgagtcaa agaatcaaca acttggcctg gtctgtaaaa
    43261 taagggacat ccacgtttat ctatcacctt aatattgtct aatctcactt ggtaaaagga
    43321 tcgtttctct cgtaccgtca tcataggcgt atacttgagc aggaaattgg ttcgaatagg
    43381 caaacggcaa atcaatatcc atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca
    43441 cttttccacg attttcgatc aaatcaaagg ttaatatatt ttctaatgga atgactccct
    43501 taagatctag gtcaataata aaccatatac tgtcaatcaa ttcatctggc aaacttgata
    43561 cgacaccaac tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac atctgtttaa
    43621 cctccttcga atcaacgaat gttttatttt tataattaat cctaggataa actagaaata
    43681 cttacttcaa cgagggttca ttctaataaa gaatcaggaa aaatgcaaca ataattgaat
    43741 actttttttc aaagtataaa tttttataaa ggtacgataa aagtatgcga ggaaaagctt
    43801 ggtaaattag gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa
    43861 taaacgatta gaaataaaaa aggcgtgtta aaagctgctg acccgccgct tctaacacca
    43921 cctgattgtt tcagtattat tcttctgttg aaccgatcac ttctggttcg ccaccttcac
    43981 ctgtgctttc ttcttcttca ctttgagcga ctacagcagc gatttgctct tcactgtcgg
    44041 tcacgaattt gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa tcacctaatt
    44101 ccaagtttgt cacgtcaact tcaattcgtt caggcaattt gtctggtgta gcagatacga
    44161 ctactgtata caagttttga gctaacactc caccagattt agcacccact gattcgccaa
    44221 caagaacgac ttctgtttcg acttctgttt cttccgacat gtttacagat agaaattctg
    44281 catgttcgaa atgattggta aaagtgttcg tttggatctt gtgaaccaat gtattgactt
    44341 tttgtccatc gatgtccatc gtgatgactg tgttcgctcc atgttcacgc aacaatttgc
    44401 ttagtgtcaa accatcgaat gagatagggg tactttctac tttataccca taaacaacag
    44461 ctggaacttt cccttcgtga cgaagtttgt ttcttaatga acggggacgt acttctcttt
    44521 tacttacttc taatgatact gacataacca aaattcctcc ttaaaatgtg actgtctgct
    44581 ttggtaacgg acagatttta tttaagtgaa ttctaaaggc taatctcgtg cacatcatcg
    44641 aaggcagggc gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc ttagaaagtc
    44701 aggattcaac ttttctccac caggtcatta cgctgtgtat gagaaatcca attcacttta
    44761 ctatttaacc gtaacgtgaa ctgttaatta agtcaaggaa taactatgat gatgtatttg
    44821 caagcaagaa tattcaaggt ataattaatt agtagtaata agaaaggaaa cagcatgcgt
    44881 ttagataaat taatagaaga aaaattagaa acgacacgca aggaaatgaa gcggctgttt
    44941 gctttgaagc aagtgatgat agatggcaca gtcgaatttc gacaaaatcg aaatgtagat
    45001 agtttactgc atcaaatcga agtagcaggt gaacacttag aaacaaatga gtcctattat
    45061 cttctcaata aacctgaaaa agctgttaca gctgtatcag atccagaaaa gacgacagta
    45121 atcgatctga ttgcaagtgc cgatcgtttc caaccattat atccagtggg aagactagat
    45181 agagatacaa caggactatt gctgatcact tcgaatggac aattgggata tgaaatgcta
    45241 cttccagaaa aaaaggtttc aaagacttat caagtagttg tgaacgaaag ggtgacaccg
    45301 gcagacgtaa cagcatttga aaatggaatc gtattcgctg atgggtatca atgtaagccg
    45361 gctgatttaa aaatactcaa agcagacaag caccaaagta cggttttatt gacgatccaa
    45421 gaaggaaaat tccatcaagt caaaaagatg ttcttagccc aaggaaagaa agtgaccgca
    45481 ttgaaacggc taagcatggg accattggaa ttagatgaac agctaggagt aggcgagtat
    45541 cgatcgttga cattagcaga gatgaaaaag ttaagtattt attttaaata ggaggtcaaa
    45601 aagatgaaat ataaaacttt attgtttgat gtagatgata cattgttaga ttttcaatta
    45661 actgaagcca atgctttaag tgctttgttt gcagaacaag gaatcacatt gacaccagaa
    45721 atcgaaacca gctataaggc tttgaatcat catttatggc aggagtttga aaaaggcaga
    45781 ctatcacgag aagaaataat aggcagtcgt tttgggttac ttttccagca atttgggaaa
    45841 acagtagata gtgatcaaat ggaaaaacgc taccgccact acttgaacca aggtcatgat
    45901 cttgttccag gaagtctaga aatcctgaaa aaggtccatc cgcatgctga tctttatatt
    45961 gtgacgaacg gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg aatgcttgat
    46021 tacttcaagg atatctttgt ctcagaagct gtcggagcgc aaaagccgat gaaagaattt
    46081 tttgatcatg tcttttccca cattcctaat tttgataaaa agcaaactgt gatcattggt
    46141 gattctttga cttctgatat caaaggagga aatatggcag ggatcgatac gatttggttc
    46201 aatagaaata aattaccaga aatcgaagaa atccagccta cttatcgaat cgattcatta
    46261 gaggaacttt attcattatt ggaaattgaa aaggattaat tttcagaaaa atatagcatt
    46321 ttcgtgtttt ttcgctataa tagattagaa tgaaaaaacg gatacgaaag aggttttagt
    46381 caatgacaaa aagcaagcca attatcattg gtgtgacggg tggatccgga agcggaaaga
    46441 caagtgtcag ccgtgcgatt ttcaacaact tcccggacca ttccatcatg atgcttgaac
    46501 aagattctta ttacaaggat cagagccatt tgagttttga agaacgattg aacacaaatt
    46561 acgatcaccc atttgccttt gataatgatc tgctgattca gcatgtaggc gacttactga
    46621 actataaagc cattgaaaaa cccgtgtatg actatgtagc gcatacaaga agtcaggcaa
    46681 cgatcattca ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt ttagaagacg
    46741 aacgtctgcg tgacttgatg gatatcaaag tatacgtaga tacagatgat gacattcgta
    46801 tcattcgtcg tatcaaacgg gatatggaag aacggggacg cacattggac tccgtgatcg
    46861 aacaatattt gacagttgtt aaaccgatgt atcatcaatt tatcgaacca acgaagcgct
    46921 atgcggatat cattgttcca gaaggcggag aaaaccacgt tgctattgat ttgattacaa
    46981 caaaagtagc tagtttctta aaccataaat agtgcgcagc gaaacagttg aacggactgc
    47041 gaccgtttga aaagcaagga ttctgctttt tgaacagtca ccagccaact gtttttttat
    47101 ttataacaag aggttgtgaa aacagcatct tgacctaagt attaaaaaga agaaacggaa
    47161 aatagctgtt tatattttac cgttttttga gctaataccg aaggtcagct gattgagcac
    47221 cgtgaagtag aggttgtaaa aaagctgtct ttgcatcaag cgataagagc atccaaataa
    47281 aacagctcct cacgtttttc gttgggttgg gcttattatc gtagatgcaa gcttttgaac
    47341 accgtttaac gagaggttgt aacagaagtg gtcagatgca ataaataaga agaaacttac
    47401 gaaaattatt tctcgaattt gtgtgagatt tcgacttatt tttttgaaga agctcttttg
    47461 tcgcaccccc
//
LOCUS       DAZUKD010000005        46202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000005 DAZUKD010000000
VERSION     DAZUKD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.005"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(448..978)
                     /locus_tag="ACG8N9_000239"
     CDS             complement(448..978)
                     /locus_tag="ACG8N9_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4895876.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1083..1634
                     /locus_tag="ACG8N9_000240"
     CDS             1083..1634
                     /locus_tag="ACG8N9_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4895877.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1787..2260)
                     /locus_tag="ACG8N9_000241"
     CDS             complement(1787..2260)
                     /locus_tag="ACG8N9_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4895878.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2398..3870
                     /locus_tag="ACG8N9_000242"
     CDS             2398..3870
                     /locus_tag="ACG8N9_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4895879.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3867..4577
                     /locus_tag="ACG8N9_000243"
     CDS             3867..4577
                     /locus_tag="ACG8N9_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4895880.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            4929..5213
                     /locus_tag="ACG8N9_000244"
     CDS             4929..5213
                     /locus_tag="ACG8N9_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4895881.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(5494..5913)
                     /locus_tag="ACG8N9_000245"
     CDS             complement(5494..5913)
                     /locus_tag="ACG8N9_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4895882.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(5915..6499)
                     /locus_tag="ACG8N9_000246"
     CDS             complement(5915..6499)
                     /locus_tag="ACG8N9_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4895883.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(6546..8021)
                     /locus_tag="ACG8N9_000247"
     CDS             complement(6546..8021)
                     /locus_tag="ACG8N9_000247"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4895884.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8202..8513
                     /locus_tag="ACG8N9_000248"
     CDS             8202..8513
                     /locus_tag="ACG8N9_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895885.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(8628..10964)
                     /locus_tag="ACG8N9_000249"
     CDS             complement(8628..10964)
                     /locus_tag="ACG8N9_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4895886.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            11106..11774
                     /locus_tag="ACG8N9_000250"
     CDS             11106..11774
                     /locus_tag="ACG8N9_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4895887.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11777..12820
                     /locus_tag="ACG8N9_000251"
     CDS             11777..12820
                     /locus_tag="ACG8N9_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4895888.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13057..13938)
                     /locus_tag="ACG8N9_000252"
     CDS             complement(13057..13938)
                     /locus_tag="ACG8N9_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4895889.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            14097..14825
                     /locus_tag="ACG8N9_000253"
     CDS             14097..14825
                     /locus_tag="ACG8N9_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4895890.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            14981..15298
                     /locus_tag="ACG8N9_000254"
     CDS             14981..15298
                     /locus_tag="ACG8N9_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4895891.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            15291..15899
                     /locus_tag="ACG8N9_000255"
     CDS             15291..15899
                     /locus_tag="ACG8N9_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4895892.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            15954..16745
                     /locus_tag="ACG8N9_000256"
     CDS             15954..16745
                     /locus_tag="ACG8N9_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4895893.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            16883..17371
                     /locus_tag="ACG8N9_000257"
     CDS             16883..17371
                     /locus_tag="ACG8N9_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4895894.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            17422..18294
                     /locus_tag="ACG8N9_000258"
     CDS             17422..18294
                     /locus_tag="ACG8N9_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4895895.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            18470..18832
                     /locus_tag="ACG8N9_000259"
     CDS             18470..18832
                     /locus_tag="ACG8N9_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4895896.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            18841..19593
                     /locus_tag="ACG8N9_000260"
     CDS             18841..19593
                     /locus_tag="ACG8N9_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4895897.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            19607..21409
                     /locus_tag="ACG8N9_000261"
     CDS             19607..21409
                     /locus_tag="ACG8N9_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4895898.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            21686..22726
                     /locus_tag="ACG8N9_000262"
     CDS             21686..22726
                     /locus_tag="ACG8N9_000262"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4895899.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(22778..24922)
                     /locus_tag="ACG8N9_000263"
     CDS             complement(22778..24922)
                     /locus_tag="ACG8N9_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4895900.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(25279..26652)
                     /locus_tag="ACG8N9_000264"
     CDS             complement(25279..26652)
                     /locus_tag="ACG8N9_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4895901.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSSKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(26652..27047)
                     /gene="rbsD"
                     /locus_tag="ACG8N9_000265"
     CDS             complement(26652..27047)
                     /gene="rbsD"
                     /locus_tag="ACG8N9_000265"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4895902.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(27075..27992)
                     /gene="rbsK"
                     /locus_tag="ACG8N9_000266"
     CDS             complement(27075..27992)
                     /gene="rbsK"
                     /locus_tag="ACG8N9_000266"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4895903.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(28100..29113)
                     /locus_tag="ACG8N9_000267"
     CDS             complement(28100..29113)
                     /locus_tag="ACG8N9_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4895904.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(29194..29793)
                     /locus_tag="ACG8N9_000268"
     CDS             complement(29194..29793)
                     /locus_tag="ACG8N9_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4895905.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            29979..30680
                     /locus_tag="ACG8N9_000269"
     CDS             29979..30680
                     /locus_tag="ACG8N9_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4895906.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            30690..33401
                     /locus_tag="ACG8N9_000270"
     CDS             30690..33401
                     /locus_tag="ACG8N9_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4895907.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            33852..35480
                     /gene="treC"
                     /locus_tag="ACG8N9_000271"
     CDS             33852..35480
                     /gene="treC"
                     /locus_tag="ACG8N9_000271"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4895908.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(35540..36721)
                     /locus_tag="ACG8N9_000272"
     CDS             complement(35540..36721)
                     /locus_tag="ACG8N9_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4895909.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            36973..38496
                     /locus_tag="ACG8N9_000273"
     CDS             36973..38496
                     /locus_tag="ACG8N9_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4895910.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(38565..39293)
                     /locus_tag="ACG8N9_000274"
     CDS             complement(38565..39293)
                     /locus_tag="ACG8N9_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4895911.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(39306..40586)
                     /locus_tag="ACG8N9_000275"
     CDS             complement(39306..40586)
                     /locus_tag="ACG8N9_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4895912.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVALIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(40727..41329)
                     /locus_tag="ACG8N9_000276"
     CDS             complement(40727..41329)
                     /locus_tag="ACG8N9_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4895913.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            41574..41780
                     /locus_tag="ACG8N9_000277"
     CDS             41574..41780
                     /locus_tag="ACG8N9_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895914.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            42092..42811
                     /locus_tag="ACG8N9_000278"
     CDS             42092..42811
                     /locus_tag="ACG8N9_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4895915.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(42892..43575)
                     /locus_tag="ACG8N9_000279"
     CDS             complement(42892..43575)
                     /locus_tag="ACG8N9_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4895916.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            43793..45979
                     /locus_tag="ACG8N9_000280"
     CDS             43793..45979
                     /locus_tag="ACG8N9_000280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4895917.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        14945 a         8203 c         9229 g        13825 t
ORIGIN      
        1 tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt
       61 tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
      121 ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga
      181 tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag
      241 cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct
      301 gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
      361 aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc
      421 tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta
      481 ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc
      541 attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt
      601 tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa
      661 agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag
      721 tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt
      781 gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg
      841 gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca
      901 ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa
      961 ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac
     1021 atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac
     1081 tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga
     1141 tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa
     1201 aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc
     1261 aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga
     1321 aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg
     1381 aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta
     1441 ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa
     1501 cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc
     1561 atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta
     1621 accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa
     1681 acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt
     1741 agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta
     1801 acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg
     1861 tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta
     1921 aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac
     1981 ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa
     2041 atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt
     2101 ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc
     2161 ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt
     2221 gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc
     2281 tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg
     2341 aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg
     2401 agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt
     2461 gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt
     2521 cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt
     2581 ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt
     2641 tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt
     2701 acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga
     2761 gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga
     2821 tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta
     2881 tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc
     2941 gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat
     3001 cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag tttactttac
     3061 ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt
     3121 gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc
     3181 ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta
     3241 acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt
     3301 ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct
     3361 atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca
     3421 gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca
     3481 gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg
     3541 ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg
     3601 gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg
     3661 ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta
     3721 gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta
     3781 gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct
     3841 gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg
     3901 cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt
     3961 tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg
     4021 gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag
     4081 gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc
     4141 gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag
     4201 tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa
     4261 acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag
     4321 tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt
     4381 tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc
     4441 aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac
     4501 aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag
     4561 aagcaaaagg cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc
     4621 atcaagcagt aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct
     4681 tagtgccgta gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt
     4741 taactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt
     4801 gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca
     4861 agacttttgt cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga
     4921 gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca
     4981 agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag
     5041 ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg
     5101 tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa
     5161 acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt
     5221 tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt
     5281 agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact
     5341 cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg
     5401 gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact
     5461 caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata
     5521 ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc
     5581 taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg
     5641 gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt
     5701 ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg
     5761 catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt
     5821 taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag
     5881 tttggttact tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat
     5941 gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt
     6001 ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc
     6061 gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa
     6121 tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc
     6181 ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat
     6241 cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat
     6301 caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg
     6361 cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta
     6421 ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc
     6481 gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc
     6541 attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa
     6601 tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg
     6661 aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc
     6721 attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact
     6781 acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct
     6841 ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt
     6901 tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca
     6961 tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga
     7021 ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg
     7081 tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca
     7141 gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt
     7201 gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca
     7261 tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat
     7321 agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct
     7381 ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt
     7441 tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct
     7501 tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag
     7561 actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa
     7621 atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg
     7681 tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga
     7741 ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt
     7801 ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct
     7861 tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat
     7921 tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt
     7981 tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt
     8041 gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa
     8101 agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta
     8161 tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt
     8221 catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa
     8281 acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat
     8341 ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga
     8401 ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt
     8461 atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa
     8521 ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac
     8581 tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc
     8641 aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc
     8701 gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca
     8761 ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca
     8821 ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg
     8881 cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt
     8941 tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc
     9001 cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc
     9061 atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa
     9121 tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata
     9181 cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca
     9241 actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat
     9301 tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat
     9361 aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca
     9421 gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt
     9481 tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa
     9541 tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta
     9601 ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca
     9661 agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa
     9721 cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta
     9781 tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga
     9841 ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt
     9901 acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg
     9961 acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca
    10021 tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt
    10081 tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg
    10141 attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt
    10201 cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag
    10261 ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca
    10321 tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc
    10381 atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt
    10441 tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt
    10501 gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc
    10561 atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct
    10621 ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca
    10681 atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca
    10741 ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga atcataatta
    10801 tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc
    10861 gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt
    10921 tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg
    10981 tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata
    11041 aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga
    11101 gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt
    11161 ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga
    11221 aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg
    11281 atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga
    11341 ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt
    11401 atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac
    11461 gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg
    11521 cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc
    11581 tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata
    11641 tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa
    11701 ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata
    11761 aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag
    11821 tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt
    11881 gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt
    11941 cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg
    12001 tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt
    12061 ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt
    12121 caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag
    12181 tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca
    12241 agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat
    12301 cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg
    12361 tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt
    12421 ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa
    12481 ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat
    12541 aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat
    12601 tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt
    12661 tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc
    12721 actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg
    12781 agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta
    12841 aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt
    12901 cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga
    12961 ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct
    13021 ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata
    13081 ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata
    13141 actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac
    13201 tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc
    13261 gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag
    13321 tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc
    13381 gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa
    13441 atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc
    13501 aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac
    13561 ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa
    13621 acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt
    13681 cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata
    13741 tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa
    13801 tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt
    13861 gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc
    13921 gaatttatgg atatccatca tattcctcct acttattcca ataaagaata ctttaacaca
    13981 aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata
    14041 attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc
    14101 caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag
    14161 taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt
    14221 cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg
    14281 ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg
    14341 gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga
    14401 cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta
    14461 cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta
    14521 aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt
    14581 ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta
    14641 tttatccatt gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga
    14701 tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac
    14761 ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt
    14821 tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt
    14881 cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga
    14941 ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc agattccaac
    15001 tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc
    15061 cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt
    15121 gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa
    15181 ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga
    15241 atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat
    15301 atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga
    15361 tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg
    15421 aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag
    15481 aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa
    15541 tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg
    15601 ttattggggg actattggtt gctttactag cgctcatttt tagtatctat gttttatgta
    15661 tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat
    15721 cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat
    15781 ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg
    15841 ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa
    15901 ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta
    15961 ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg
    16021 ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca
    16081 aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga
    16141 taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga
    16201 ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac
    16261 aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata
    16321 atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg
    16381 gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct
    16441 cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact
    16501 tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt
    16561 tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata
    16621 ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa
    16681 agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag
    16741 attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt
    16801 gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa
    16861 agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac
    16921 agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct atgatccaga
    16981 caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt
    17041 gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt
    17101 accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa
    17161 atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga
    17221 ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc
    17281 cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg
    17341 tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga
    17401 taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca
    17461 gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg
    17521 tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg
    17581 ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga
    17641 caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa
    17701 atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg
    17761 gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag
    17821 gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt
    17881 cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga
    17941 ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta
    18001 gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct
    18061 agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt
    18121 gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc
    18181 aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca
    18241 gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact
    18301 ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt
    18361 tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg
    18421 ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact
    18481 tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta
    18541 cgtagttatg ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt
    18601 ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc ctggctatac
    18661 gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa
    18721 aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt
    18781 cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa
    18841 atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa
    18901 aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc
    18961 atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa
    19021 ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa
    19081 ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa
    19141 agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa
    19201 gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa
    19261 agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg
    19321 atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt
    19381 gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt
    19441 cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat
    19501 ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa
    19561 atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt
    19621 atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt
    19681 tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa
    19741 aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg
    19801 gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa
    19861 agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt
    19921 ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg
    19981 attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt
    20041 tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt
    20101 attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg
    20161 tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt
    20221 actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca
    20281 agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca
    20341 ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa
    20401 attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct
    20461 ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt
    20521 taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa
    20581 tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta
    20641 tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc
    20701 attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc
    20761 tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga
    20821 acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc
    20881 acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga
    20941 aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa
    21001 ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg
    21061 attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt
    21121 gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg
    21181 cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat
    21241 cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc
    21301 atacaaagat atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt
    21361 actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc
    21421 tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg
    21481 gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca
    21541 atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc
    21601 gttactcacg gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa
    21661 tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta
    21721 tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc
    21781 acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt
    21841 tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat
    21901 ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac
    21961 agacccggac actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg
    22021 gcattataca gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc
    22081 agatatggaa aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa
    22141 cgatgataca tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc
    22201 tacaaatcat gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat
    22261 actttggttg aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga
    22321 agtagtttca gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt
    22381 ctttgccagt gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt
    22441 gaaaaaacaa tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc
    22501 gatccaagta aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga
    22561 tggaccggaa atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca
    22621 agaactgcca ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca
    22681 agtacgcgag tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaaaag
    22741 ggagtgtgac agtgtcacat ccccttttta atttattcta tttctttaaa ggcagatcaa
    22801 gccagaatga tttgtctaaa cttaacaagg ttagtacagg aacagttgca gctttaactg
    22861 tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa tcccagattg
    22921 aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa aattctaatc
    22981 ctgtgattgg cacgccatct gttgtccatt tgattggcac atcgaatcgc ttagacatat
    23041 tgaggatttc agcgtttgct tcttcagttg ttgctgcttc ttttttcggt tcatcatatt
    23101 ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg cgatctgtag
    23161 cataacgtcc ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc gacttccaag
    23221 ttggtttgta gaaatcagga ttgccagtca gtccattttt gatcaagtcg gcatagtctt
    23281 ccaaactttc cttgtaagaa ggatatttac ggaattgtgc gcggatttgg tagctttgtc
    23341 cggaactatc ttgttcaagt gtattgaagg tcacgctgct tcctttatat gatcctttga
    23401 taccaaaaag gttatgattt ggttcgcttg ataataagct gtttccagat ccactttcaa
    23461 ggatagcttg agccatcatg actgatgcgt agattccttc tttttgcccg atttcctgtg
    23521 catcatctgc aatctttttc aagaactgat cagttgtttg gttttgactg aaatggaacg
    23581 agccatctaa tccatctaaa atagatgagc cgcctgtcag cgaacctgaa gcagcaggta
    23641 aacctgctaa gtttactcgg acagcaaaat caggcgtgaa atctgaaata gaagttactc
    23701 gaactttatc tcctggttga ggcgcatgga taaattgtcc gttcccaata tagataccta
    23761 catggtgagt aacaccggaa gaaggtcccc agaagacgag gtcgccaggt tgggcctgac
    23821 ttactggaat ctgcgtacct gcatattgct gttcgcctgt ccagccaccg atataatgac
    23881 cggttgcttt catatatacg taataagtca aaccagaaca gtcaaaagta tttggacctt
    23941 ttgctcccca tacatatggt tttccaagat gcttgaatgc ttctcgaacg attgcttgtt
    24001 ggacattcgt tgtatttgta ggcaatacag catcaattgg tgattttaca acaaaatcag
    24061 aagaagcaga agatggattc tctataccgc tttgatttgc tgtatttgga cgagatgaag
    24121 cattgttcgt tccgccattc gatgaaccat tgtttggatg gtttggttct ctctgagtat
    24181 ttccattgtt attcgatcca gatgggtttg tttgattcga agaattcccg ctgttcgaat
    24241 tattgttatt attgtttgaa ttatttgaac cattattatt gttgttatta cccacatctg
    24301 gtttgtttgg cgtttccgga tctggtgtac tagggacttc ttcactagga tcagttggtt
    24361 tctctgttga agtatccggt ccagctttaa attctgcagt aatagataaa ttgttcgtac
    24421 ctactgtaaa ggtctttgca tcataattta cttctttggc accattaaca gcgactgaag
    24481 caatttgttc accttcagct ggcgtaatag tgtaagttac cttcgtttcc gctaataaac
    24541 cttctaccgt tccactttcc gtaatatcca aaggttgctg ttcaggggaa tccgctgtcg
    24601 aagcagtcag ttggatctta ccaatcaaag aaggagcaat tgtaagggaa tatgtctcgg
    24661 ctggcttttc attttctgat ggatcagccg gatcaatttc agttggctcg gtcgatcctg
    24721 ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga atccgttgtt tcactagtag
    24781 ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc agctatttga ttatctacgg
    24841 caaatacggt attggtcgac aaaacagacg gagcaatcag ccaagtagca gctagcaaac
    24901 gtatcgtttt tttatttttc ataataaact agtccttttc ctttcattga ttttctgtct
    24961 tattcagcat tcattagaca ttttttggaa cagataatcg tgtcgaaaag ctttttcttt
    25021 tcataaatct gtctgtgtat tctaaataaa acaatttgta aataagcaac gagcattcat
    25081 tcgtttttct ttgacgttca ttttattgca tagaccacct ttgtttgggg ataaacaagt
    25141 ggggataaaa atggttatgc atatgtaaat tataccaaag tttggaaagt atcacaaaaa
    25201 tttataaaaa taatgatcct aaaaagaaaa agactgaaat atgtgataaa catctcatac
    25261 ttcagtcttt ttcattaact attcataaga cattaggtct tcatcaaacc cgctaacgag
    25321 agtagtgatc attgctacga ctagactaac agccagtcct gctaagtacc agaaaaaagt
    25381 accggaaatg aatgcaggca atgtggtcag actaccaaat acaaaagcga tcgctttcac
    25441 ttgggcaaat cccattacag ctccaccagc agctgcacca atgcaagcag caacaaatgg
    25501 ttttttcaat gggatcgcaa ttccgtaaac agttggttcg gtcactcccg caagtaaaat
    25561 cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc gcagaaatac
    25621 acctaaagct gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa cgattcggtc
    25681 gaacccataa gcagagatat tactgatcat gacagggttt acagcccatt ggataccaaa
    25741 tattaccaaa atattccaac caccagcgat gattgcgcca ctgatcggtc cgttaatatc
    25801 catcatccaa gtaattgcgg cagctaaacc ttcgccaagg taaacacccg caggcccgaa
    25861 taccattaac gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa atactaattg
    25921 catattttcg cgaatatatt ttttgaggaa tttttcaaca aaagaataaa agaaaatacc
    25981 taaaatcgcc ggcatgacag tagacgtata ctgcatcagt actacaggaa tccccagaaa
    26041 atcagtagct gctttgctac tgtctgaaag taaagaggtc agattcggtt caatcagtgc
    26101 tgcagcaatg acagccgcaa tatatccatt cactttaaac gttttggcag cagaaaaagc
    26161 aagaaagatt ggcagaaaat aaaagacggc attcgacgca gcagacaaaa tatgataagt
    26221 tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa acaaccagac cttttaatat
    26281 accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca aaccaaatag
    26341 tttttcgatc ggatttagct tttctttcgg agctgaatga tcgatttgac gcgtcaattc
    26401 cttgtaagca ggagccaatc cctgatccat gattatttct gcatatctat cgagtaaaat
    26461 gaaatactta atggaaggga tttgctgttt taatttttct gcattggcta ctggctgttt
    26521 tttcaatttg atacgaagcc gagtcgcaca tggaaggaca gaatcaatat tgtcttttcc
    26581 tcccaactgt tcaatcagca tatgaatctg ctcggacagt accatctgtt caggattcat
    26641 tgtcgtttcc attagaagac cactccactc actaatataa tattggcgta aggtgtcatt
    26701 tcgcctgtac gaataaatgc atgacagtta ttcaactctt ttttcatttc ttcatgtggg
    26761 ataaagctaa tcggtgtatc tggtaacaat aatttgattt tttccaatat ttccgggttc
    26821 attgtcttta tttcttccgc caattcaatc gattgtacct caagttcttc taaaacgttt
    26881 tctaaaacag ataaaaaact tggaagaccg tttgagacag ccaaatctat tttttcagta
    26941 ccaaagggga caggcatgcc tgcatccccg atacttaatt tatcaaagtg ccccatttgt
    27001 gcgattactc gtgaaatatc tgaattgatt acttttcctt ttttcatgtt tgtttcttcc
    27061 tctcattgtt aattttagac tgctgattgt tctaaagctt cgcggtaagg gatagaaggt
    27121 tgcgcaccat aagactgcac agcaacagaa gaggctagat ttccataaag gatcgcttct
    27181 ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa tgtatcacct
    27241 gcggctgttg tgtcaacggc atccacttta aaagcaggga cgataccgtg ttctttttct
    27301 gtatgataga aagcgccggc actgcctagt gtaatgatta ctgttccaat acctaattca
    27361 tggagttttt cggctgctgc gaccaaactg ttatgatctg tcactctgat tcctgtaatg
    27421 atttctgttt ctgtttcatt tggtataatg atatctgtct tttcaagtag ttcagtagga
    27481 atcgtttcac gagctggtgc aggattcagg atcgttgttt ttcctgcgtc ttttgcaata
    27541 gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact gagcaataat aaagtcactg
    27601 tttgtaatca gttgcttgtt ttttaggact tcttgttcat ggaaagcatt atttgcccct
    27661 gcatggatca agatactatt ttctcctgct gcgtctacca taatcattgc ttgtccggtt
    27721 gtggcgcctt ggatttcagc tatcccagat gtatcgatgt tttcttttgt cagtagatct
    27781 aataactgtt gtccttcgct gtcagcccca acgcctccaa taaaagatgt ccgtgcgcct
    27841 gaacgtttgg ctgccactgc ttggttggca cctttacctc cgccagcgtg aaaaatctca
    27901 tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta aacgaagggt agtgtccata
    27961 ttaatacttc caattactgt aaccgtgttc attttacaaa cctccaacta tctcatttat
    28021 tttgatggga aaattatttt ttaaaacgtt ttagtaaaac gttttaatag ttcgataaaa
    28081 atatagcaca ccttgaaagc tatttcaagg gtgcagttga aaatcttttt tctaatcgta
    28141 caggtaatct tttttcttcc cattcttttt ctgggaattg gatccgatct aacaataact
    28201 tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt ttcggcttga
    28261 tatactcgca catatctata ttatcatagc caataatgct gtagtcttca gggatgcttt
    28321 ttccagcttc ctctaatccg cgatataatc caaaagctaa ttcatcattt aaagcaaaca
    28381 caccagttgc agcagaatca agaagttctg ctgtgatctg gtagccacct tgttttgaga
    28441 actgtgtagg taagaggatc agttggtcgt gagggatttg gtaaacatca agagcagatt
    28501 taaacccctc aatccgagcg tggacatttt ccggtggatt ttcaggatag acaagggcga
    28561 tcgtttgatg tcctagacta agcaagtgca tgcccgctag gtaaccaccg cgaaaatcgt
    28621 ctgttcgtac ggcatcacta aatccttcag attttttctg atccaaaaca atgaagggcc
    28681 ggccttgttt tttcaaattt tcgttgattg catctgtaga gacagcagaa gtcgcgataa
    28741 tgaatccatc cacacctcga cgtgtgagct cggctagata ttcgatttct ttttgatgat
    28801 cagaatcagc attacataat atcgtgacga aattctgctt atacaagata tcctcaattc
    28861 ctcgcatcag tgtactgaag aatggattcg tgatatcagg aacgagtaca ccaattgttt
    28921 tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct aattcttctt
    28981 tggcagctaa aactttctga acagttttcg gactgaattt ctccgtattt ccatttaata
    29041 tcaaggagac agttgtgatt gaaacgccgg aaagtttagc gacttctttg attgtgatct
    29101 tttttttcac catgttcata ctcctatcta aatcctgtgg ttattttccc atgaaatcaa
    29161 aaagaagtaa atgaaaatca cgtgtgccag tacttatttg ctttgttgga tagcttcgat
    29221 tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat atctttttta tgaacaattg
    29281 atgggattct ttgtcacgat cactgcaagc atcttcagca agcacgatat ggtacccgtg
    29341 ttgaaaggca tctaatgcag tagcatatac accgtttgtt gtcgctacac cgcttaaaat
    29401 gatagtttcg attccacgcc ggcgaagttg aagatcaaga gaagtaccaa aaaaagcact
    29461 tggattgtat tttgtgatag tcaacatatt atctgtatct ttcaaagaat ccttcaaaag
    29521 taaatcagtg aattctggag gtacagaagc agtcattcca cccatatcag tttgttgact
    29581 caaataaggg aaagtggaga tgtctacgtt cactaaaact ttcaaggcag cggtacgttt
    29641 caatgttctt actaacaaat cattgtttgt cagaatagat gctgcagtgt gtggagagag
    29701 tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg acaaaagcag tttttgataa
    29761 atcaaaaatc atattttcct ttaaatcatt catgatgttc cttctttcaa ataacaatgt
    29821 tttttatgta agtaaatcat aaatcgttta tttgtcaact aaattaaagg attcctttct
    29881 ttagtctaga gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat tcgatgcata
    29941 gatatagcga gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc gaagtaaaaa
    30001 atgaatacaa acgatacaaa atgggagaaa cgaccattac agcaaatgac gatatttctt
    30061 tttcaattga aaaaggcgaa ttagtgatta tacttggtcc aagtggagca ggaaagtcaa
    30121 cgattcttaa tattttaggt gggatggata cacctgatga aggtcagatc attattgatg
    30181 acacagatat tgcgcaattt tcagataaac agcttactgc ttaccgacga acagatgtag
    30241 ggttcgtttt ccaattctac aatctcgttc ctaatctaac agcaaaagaa aatgtggaat
    30301 tggcaacgga agtttctcct gatgcgcttg atcctgtaga ggtattgagg caagtgggcc
    30361 tagctcatcg attgaataac tttccgtccc aattatcagg aggcgaacag cagagggttt
    30421 caattgctcg tgcactagct aaaaatccca aacttctttt atgtgatgaa ccgacaggcg
    30481 cgctggattt tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct agcagacaac
    30541 acggcaatac cgttttgatc attacacata attctgcttt agcgcccatc gctgatagag
    30601 tcattcacat taatgatgct aaagtaaggt ctgttgaatt aaatgatcat ccttcatcca
    30661 ttgatgagat tgtatggtag ggggatttta tgaaaacaag aacatatctt aaagcaggtt
    30721 ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat catccttatt gtcatgctag
    30781 gaacactttt atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa acgatggatc
    30841 actatgttgg tacagcagga ctttctgatt tgcaaatcgt ttctactggt ggcttgacac
    30901 aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca ggaaaacaga
    30961 tctattattc caatactggc aaaaacgaag tgattcaaat attttcttat aacaaaaata
    31021 acaagcagaa caagctacaa ttaactgatg gaaaactccc ggaaaaacca aaccagcttg
    31081 tattggatga aaaagcggaa gatgaagggt ataaaatcgg tgatatttat cagattgatt
    31141 cagatgaatt gaaagaaaaa gagtatacca tcacgggctt tgtccgttct ccattgttca
    31201 tcaacaattt ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat tattttgtct
    31261 atttacctga atccgttttt aaaacagata tttattctgt cttatatctt gattttacaa
    31321 acgtaaagaa tttgaatacg tacagcaaag cttacaaaga taaaatggag caaaatcaag
    31381 aaaaggcaga aaagtattta caagatcgac ccgaagaacg cttagaagag ctgaaaaaat
    31441 cggccaacga agaattagaa ccggcacagc aaaaaatagc ggatggcaaa gcacaaacaa
    31501 cgcaagcaag aacacggtta gaagatgcta aaaaacagtt agcgcaacag tcagcaacta
    31561 tccagcagct tcctgaagcg caacgaacaa tcgctgttca aacattatct caacaagaag
    31621 ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga acgtgaactg gcctctgcag
    31681 aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt aactaaacca tcatacctat
    31741 acaatgaacg ttcagaaaat gtcggttttc aagaatttgg tgatctgtct gatcgtatcg
    31801 ctgcgattgc caatgttttt cctgttttct ttttctttat tgccgcattg atcaccttta
    31861 cgacaatgac acgtatggta gaagaaaacc gcagggaaat cgggacatta aaggcgttag
    31921 gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta gcctcaggga
    31981 taggtattgt tttaggaacg atattaggaa cgaatctttt gccaaggatc atttttgagc
    32041 tgtctaacga gcggtatgat attgggagtg cgcttatttt ttatgactgg ccgccgattt
    32101 tacaagcagc tgccgcattc ttcattgccg catttggcgc tgcaatgatc gttttattca
    32161 aagatttaag agaacggcct gctgcattgc ttcaaccgaa agcaccgaag ccagggaaac
    32221 gcatttttct tgaatacatc actccgctgt ggtcacgcct tagctttaat caaaaaatca
    32281 gttaccgaaa tctcttccga tataagtcaa gaatgcttat ggcgattatc ggaattgctg
    32341 gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt tctgtttcag
    32401 ccaaacaatt tggaccgatc attgactatc aagcaatcgt tacattggat tcatccacgg
    32461 aagatagtcg tcaaaaagca gcggatatgc tgcagaagca agaaaaagta aatgagatcc
    32521 tagctattca tactcaaacc gtagaattga gaaaaaaagg acatgcttcg caaagtctga
    32581 cagtgatcgt accaagtaaa acagctgatt tggatcgttt tatccattta actgatcttg
    32641 aagagaaccc acgctccttg cctaaaacag gaatcgctat tacgcaaaaa gtagcagaac
    32701 tatttgactt atctacagga gatcatattt cgctttatga tgaagatcaa aaagaatgga
    32761 aagtcgaagt aaaagaaatc gtgaaaaact atctaggtag ctttatttat atgtcccctg
    32821 aatattatgc ttcagttact gatcaatcga tgatggataa tgcatttttg ctgcgtacag
    32881 ataagatgac aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc acagacgctg
    32941 tgacaaatac gtctttcatg tccaaacaaa tcgaaacgca agaagaatca atggcgaact
    33001 tagactcggt tgtcttaatc tttgtcgtat tatcaggact gctggctttt atcgtcttgt
    33061 ataatctaac taatatcaat atttccgagc gtgtacgcga attatctaca ataaaagtcc
    33121 ttggattttt tgataaagaa gtcacgatgt acattgttcg agaaaatatc atctttactc
    33181 ttttaggaat catcggagga tttggtgtag gatatatatt gactgacttc atcttgcaac
    33241 aagcgtctat ggagaatgtc atctttccat tagtgatcac atggtgggct tatgtcttat
    33301 cagcaggatt aactatcgta tttacggtta ttgtgatgat tgttacccat ttcaaattaa
    33361 agcatatcga tatgattgat gcgttgaaat cgaatgaata aaaacagagg ttgtgaaatc
    33421 agcggtttga cctgagtatt agagaaacaa acggcaaaat agtttttcat attttgccgt
    33481 tttttgagct aataccgaag gacagctgat tgaacaccat gaatcagagg ttgtaaaaaa
    33541 gccgtcctgc atcgagcaaa aagaatactc aaataaaatt agctcctcat gttttacatt
    33601 tgagaagact gattgccgta gataaaagct tttgaacacc atttatagaa gaaacaagaa
    33661 tattttgcta ttttaaacaa gaggatagac aaatacatgt ctgatctctt gttttttatt
    33721 gggcaacaga cctcttagaa tgtataaaga taaattttac aaacgctttc ggttcagtta
    33781 taataataaa tgataacttg tatatataag ttgtggtagg taataaatca gttgaatgga
    33841 ggtaaatatc catgacattt aaagacaaag tcatttacca aatctatccc aaatcttaca
    33901 aagacagtaa tggtgatgga attggagatt taaaaggaat caaagaaaaa ctcccgtatc
    33961 tgaatgatct aggcatcgac atgatttggc taaaccctat ttatccaagc cctcaaaaag
    34021 acaacggtta tgatatctct gattacacag ccatcgatcc tatctttggt acgatggagg
    34081 aattcgaaca gctaatagcc gaagcaaaga aataccggat agaaatcatg ctcgatatgg
    34141 tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa agcactagca ggggaccagc
    34201 attatcagga cttcttcatt ttaagagaag agccaacgga ttgggtatca aaatttggtg
    34261 gaagtgcttg gtcgccattt ggagatacgg ggaaatatta tttgcactta tatgatcgga
    34321 cacaagctga cttgaactgg cggaaccaag cagtaagaga ggaactattc aaagttgttc
    34381 gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat gttatcggta
    34441 aagacgaaca attgaaaaat aataaagaaa atgatggcaa acctgaatat acggacaaac
    34501 caatcaccca tacgtatcta aaagaattga accaagcaac gtttgggacg gattccacta
    34561 tcatcacagt cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg tatacagaac
    34621 ctagtagaga agaattatca atggtcttta atttccatca tttgaaagtc gattatgaaa
    34681 atgggaataa atggtctact cctccatttg attttgcaga attaaagcga ctatttcata
    34741 catggggaga aaagatgagt gagggtaacg gctggaatgc tctatttttt aataatcatg
    34801 atcagccacg tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa caaggagcag
    34861 agatgctagc aaccatgatc catttgaacc gtgggacgcc ctatatttat atgggagaag
    34921 aaatcggcat gatcgaccca gactattcat cgatttccga ctatattgac gtagaaagtt
    34981 tgaatgctta taagttactc cttgaacaag gattcacaga aaaagaagcg tttcgtcgga
    35041 tcaaagcaaa atctcgcgat aactctcgaa cacccatgca atggtcagac acagaacaag
    35101 caggatttac tacagggaaa ccttggttaa aagttggagg caagctagaa gagattaatg
    35161 tagaaaaaga actatcttct gaagattcta ttttgtccta ttacaagaaa ttgattcggc
    35221 tgagaaaaac atatccaatc gttgcacaag gagattatca tgcttatgaa gcaaatcatc
    35281 cggaagttta tggctttctt cgccaatttg aaggacagca gctgcttgta ttaacaaatt
    35341 tctatgataa agatacaagg atcgccatcc cagaaggatt cacagaagcc aatattctta
    35401 tcgataatta ttccgatact gttcttcatc aaagtctgga gctaaaacca taccaggcaa
    35461 ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag ctattctcgt
    35521 aagatcgacg ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa gagttttcat
    35581 actctccttt atcgccataa aatttattgt agcgttgttt gaatttacgg aaaataaaag
    35641 taacgagtaa tttgactaac gtatagatag gaataccaaa aataacgccc acaattccca
    35701 ttagatcacc catgacaagt aaaacgatta agatcgtaat cggatggatc tgtaaacgat
    35761 cacccattac tcgaggaatc accaagtcac cgtggagaag ttgtacggca aaccagacaa
    35821 tgacaaattt gacagccata aaagtagagt cttggaatgc aatgatcaaa cctggaaaga
    35881 aggcgataaa cggcccaata tatggaatga tacataacaa cccagcagaa atagctaaaa
    35941 cactagcaaa ttctaatccg atcagtaaga aacaaaccca gtagactgct ccaaggataa
    36001 atgaagcaat gatttgcccc tttagaaatg aacccaattg ttgattagca atctttgtca
    36061 aatttttaaa atctgtacga aatgctggtg ggacaatcgc taaaactgac tgataaaatt
    36121 tttgaggatc tctcaaaagg aaaaaagcga caattggacc agtaaataaa gtaataaaaa
    36181 tagctgtaac agttgaaaaa atgttgctga tccgttgtgc gccactttcc caataatttc
    36241 caataaatcc agctatatca gacgtcacgt cgtcaataga agcgaaaaat tgattaaatg
    36301 aatcggcaaa aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa tcttgaaaca
    36361 aatctggcaa actactgatc aatgtctttg cctgttcttc aagaaaagga ataagccata
    36421 tgcttccaag ccccaataaa aataaaataa tcacataaac gataatgact gacaagttcc
    36481 tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa aagataatac aagatcagtg
    36541 caaacaataa tgggggcaag atagtagcga taatgattcc gacaggcttg aatataaacg
    36601 agatctgatg gaaaaaataa atgatcagcg caagtaagat aactattcct aaaacatagt
    36661 aagaggtttt tcctcctaaa aagcggatca aacgattcgt tgtatctgtt tcttttttca
    36721 ctctttttcc tcctttatct ataaattcat tatagacaaa agcgggtata aaaggaaaat
    36781 gatagaggtt gattgcaaaa atatcgtcaa tcattcatat aaactttctc atcaattaaa
    36841 ataagaaaac gcttgacagt tcttttaacc atatgataaa ttaaatcctg ttgctaaaac
    36901 gtaactatta cggtttgcga aaagcgaatc ggaacattca aatgaaagaa gatgagaaga
    36961 ggagtgtcat aaatgaaacg attgataggt atattggcca tgctggtttt agcaggtatg
    37021 ttaactgctt gcggggcaag tgggaaagcc gaggacagta aagaaaaatt gagcgtgatg
    37081 acgacctttt atcccatgta tgattttaca aaagcaatcg tcggggatga aggagaagta
    37141 gagttattga ttccagcagg gactgattcc cacgattatg aaccttctgc taaagacatg
    37201 gcaaagatcc aagatacaga tatctttgtt tataatgatg aaaacatgga aacatgggtc
    37261 cccgcaatcc aaaaaacatt acaagaaggc aatgtccata cgataaaagc gacagaaggt
    37321 atgcttttac ttccgggaag tgaagaagga catgatcatg accatgaaca cggggaagaa
    37381 ggccatacac atgagctaga cccacatgta tggctagctc caagtttagc aatcaagcag
    37441 gtagcaaaca tacgtgatca gctgattgaa gcttacccag aaaaacagga agtatggaca
    37501 aaaaatgcag cagcctatac cgagaaactt caagcactcc atcagcttta tcaagaaacc
    37561 tttaagcaag caaaacaaag atcttttgtc acgcaacatg ctgcattcaa ctatctagcg
    37621 ttagaatatg gattgaatca agtatcgatt gctggtcttt cttctagtga agaaccgtct
    37681 gcggcgagaa ttgctgaact gaaacacttt gttaaagaac atggaatcaa ctatatttat
    37741 tttgaagaaa acgccaaaga tagtattgcc agaacgttag caaacgaagc aggtgtatct
    37801 ttagaagtat tgaatccttt ggaaggattg acgaatgaac aaatagaaaa tggagaaaac
    37861 tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga gacagaaaac
    37921 ccgctagaag atactttgac ccccaaaaaa gaaaagagcg tctacaacgg ttattttgaa
    37981 gacgaggatg tacaagatcg ctctctttca gattgggcag gaacttggca aacagtagat
    38041 acgtatgtag aagatgggac ttttgatcct gtttttgaat ataaagaaaa attgaatcag
    38101 gataagacag cacaagaata taaagaatat tatacgaagg gctatcaaac agatattgct
    38161 tcaatcaaga tcagtgagaa agagatggca tttacgttta agaatggaac aacgaaacgt
    38221 tcaaattatc gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa ccgtggcgta
    38281 aggtatctat tcgaagcaga agataaggaa agtggcgctt ttagatatat tcaattcagt
    38341 gatcacacga ttgcaccggc tgactccgaa cattttcatt tatatgcagg aaatgaaagt
    38401 caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga tacaatggat
    38461 ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa ccgaagggtt
    38521 tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga aatcaatttc
    38581 tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac ttctctggtt
    38641 tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg acaacggata
    38701 aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa ttgggcatga
    38761 gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata ttggatgtct
    38821 ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc tttttggatt
    38881 tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt ttcttttaaa
    38941 tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt taagattttc
    39001 atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc taattcacgg
    39061 atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat atctaaaaag
    39121 taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc cggatcagag
    39181 gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt gatcaccgtt
    39241 gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat catatcatta
    39301 ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc aattgatgtt
    39361 tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa gccaaggcct
    39421 tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt taacggtaat
    39481 gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt gagtatggtc
    39541 aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc caagataata
    39601 ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg ctcaggaaaa
    39661 tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga cagtaaacta
    39721 cggatctcag gaactttcat attatccaac atacttagat tggccggaaa aaggttcatc
    39781 atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc taagtcgcct
    39841 tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt gaattctctc
    39901 aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct gacttgttgc
    39961 tctaataact gatcagctaa tttcttatta gagattttcc aataataaac caagatgatc
    40021 agggcaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac atcaggtgtt
    40081 tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat caatgtcccg
    40141 atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga tttttttaaa
    40201 caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata ctgcaatgaa
    40261 tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct tgttaagtga
    40321 tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg ttgacgcgaa
    40381 gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc agtaaaaatc
    40441 cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac tcccgcaaac
    40501 agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta aatcaaatat
    40561 tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt accaaattat
    40621 agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag aactcatacc
    40681 aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa tgattgtttt
    40741 tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc aacgtagcta
    40801 tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag aaaaataacg
    40861 taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg ggacgtttct
    40921 cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt aacaaaaagc
    40981 agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga tgtttcatca
    41041 ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat tcagagtcgg
    41101 aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg attcctgttt
    41161 ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt ccaagattat
    41221 tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct tccgatttgt
    41281 ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc tcatacgtac
    41341 ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca taaaaagtgg
    41401 ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa gaaccttacc
    41461 aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt gctttttcga
    41521 gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt cttatggaaa
    41581 atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta tttgaatcac
    41641 caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt gtcatgaaat
    41701 gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt gtacgcaaat
    41761 caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag gttgtgagtc
    41821 tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca tgttttatga
    41881 atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa aaagggctgc
    41941 gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg gctactttta
    42001 gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa atgaagtagt
    42061 aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca ttgttgcagg
    42121 aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt tagaaacaaa
    42181 acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt ctaatccaga
    42241 aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg caatcaaaga
    42301 tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata gtatgctgtt
    42361 tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt atgaaggtct
    42421 tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac cagatctact
    42481 ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa gaggccggga
    42541 atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac tcaatcgacg
    42601 atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg atggggacaa
    42661 atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa tcaagatgcg
    42721 ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta caaataaccc
    42781 gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt gctcactcgc
    42841 agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag atcaaatagt
    42901 gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg ccatgatcaa
    42961 aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac agaaaacaag
    43021 gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa atcgatcaaa
    43081 atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc cttttaaatt
    43141 tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag ctacccactg
    43201 gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat ggacgggtgt
    43261 caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc gccagaaatg
    43321 tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc ctaagaaaaa
    43381 gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa atgcttggat
    43441 gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt ggatataagt
    43501 ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac gcccctcgtg
    43561 aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc cttatcttag
    43621 cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta aaatcatatc gcttcccatt
    43681 ttcattttta gaaaggtctg ttagtataaa gaaaaaatag acaacgaaac tttaaataac
    43741 caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc agttgaatga
    43801 aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt ataaaaagct
    43861 tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg cttatggcga
    43921 agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa acagctcttt
    43981 ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa aagcagctat
    44041 cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg gcaaaacgca
    44101 aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg aagaacgcat
    44161 actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag tagataattt
    44221 cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt ttaggatata
    44281 caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag gagacttttc
    44341 aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta aatataatct
    44401 ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact attggcagaa
    44461 tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg attccattga
    44521 taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg aactccaaga
    44581 acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc tgaaaagcgc
    44641 tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc tattcattga
    44701 tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga caaaggaaaa
    44761 caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat tcagaggatc
    44821 agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc ttttcttatt
    44881 aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga tcagctataa
    44941 taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag gtgttatgga
    45001 agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac aaattcagac
    45061 atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat cagcttcaca
    45121 aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac tggaagcaga
    45181 tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc aaaaaattgt
    45241 tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc aattgctccg
    45301 gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact tttctcctaa
    45361 atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt taaaatcgaa
    45421 atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc atcaagaaat
    45481 ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa tcgaccagag
    45541 tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa gactccaagc
    45601 cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga ttgcttacag
    45661 acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag gtcttagtta
    45721 ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg gagcgacaga
    45781 aaagaaagta atcgacttta atgaaataaa agcagaacca gtaacgacga tagaagaaga
    45841 acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc tatgtgtacc
    45901 tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa ccgggagtcg
    45961 tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt ttcaagaaag
    46021 aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct tattttcgaa
    46081 gaagctattt ctggaacaca gtttattcgg aaaagggagg tgtgacaaga attttgtcac
    46141 acctccctta tttattttga aaatgagaat actattatca aaatagagaa tatatagaaa
    46201 aa
//
LOCUS       DAZUKD010000006        43626 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000006 DAZUKD010000000
VERSION     DAZUKD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.006"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..2647)
                     /locus_tag="ACG8N9_000281"
     CDS             complement(266..2647)
                     /locus_tag="ACG8N9_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4895918.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2637..3284)
                     /gene="recU"
                     /locus_tag="ACG8N9_000282"
     CDS             complement(2637..3284)
                     /gene="recU"
                     /locus_tag="ACG8N9_000282"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4895919.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3348..3887
                     /locus_tag="ACG8N9_000283"
     CDS             3348..3887
                     /locus_tag="ACG8N9_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4895920.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3977..4396
                     /gene="gpsB"
                     /locus_tag="ACG8N9_000284"
     CDS             3977..4396
                     /gene="gpsB"
                     /locus_tag="ACG8N9_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4895921.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4418..4785
                     /gene="rnpB"
                     /locus_tag="ACG8N9_000285"
     ncRNA           4418..4785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8N9_000285"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4994..6160
                     /locus_tag="ACG8N9_000286"
     CDS             4994..6160
                     /locus_tag="ACG8N9_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4895922.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6206..7702
                     /locus_tag="ACG8N9_000287"
     CDS             6206..7702
                     /locus_tag="ACG8N9_000287"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4895923.1"
                     /translation="MKEKEFLQEVKEINLLQKALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7783..8466
                     /locus_tag="ACG8N9_000288"
     CDS             7783..8466
                     /locus_tag="ACG8N9_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4895924.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9111..10301
                     /locus_tag="ACG8N9_000289"
     CDS             9111..10301
                     /locus_tag="ACG8N9_000289"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4895925.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10403..11356
                     /locus_tag="ACG8N9_000290"
     CDS             10403..11356
                     /locus_tag="ACG8N9_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4895926.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11425..12774
                     /locus_tag="ACG8N9_000291"
     CDS             11425..12774
                     /locus_tag="ACG8N9_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4895927.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12957..13280)
                     /locus_tag="ACG8N9_000292"
     CDS             complement(12957..13280)
                     /locus_tag="ACG8N9_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895928.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13409..14572
                     /locus_tag="ACG8N9_000293"
     CDS             13409..14572
                     /locus_tag="ACG8N9_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4895929.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14642..15991
                     /locus_tag="ACG8N9_000294"
     CDS             14642..15991
                     /locus_tag="ACG8N9_000294"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4895930.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16036..16467)
                     /locus_tag="ACG8N9_000295"
     CDS             complement(16036..16467)
                     /locus_tag="ACG8N9_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4895931.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16855..19497
                     /gene="alaS"
                     /locus_tag="ACG8N9_000296"
     CDS             16855..19497
                     /gene="alaS"
                     /locus_tag="ACG8N9_000296"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4895932.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19697..20293
                     /locus_tag="ACG8N9_000297"
     CDS             19697..20293
                     /locus_tag="ACG8N9_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4895933.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20415..21563
                     /locus_tag="ACG8N9_000298"
     CDS             20415..21563
                     /locus_tag="ACG8N9_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4895934.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21601..22014)
                     /locus_tag="ACG8N9_000299"
     CDS             complement(21601..22014)
                     /locus_tag="ACG8N9_000299"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4895935.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22093..22524
                     /locus_tag="ACG8N9_000300"
     CDS             22093..22524
                     /locus_tag="ACG8N9_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4895936.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22623..22856)
                     /locus_tag="ACG8N9_000301"
     CDS             complement(22623..22856)
                     /locus_tag="ACG8N9_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4895937.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23039..24709
                     /locus_tag="ACG8N9_000302"
     CDS             23039..24709
                     /locus_tag="ACG8N9_000302"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4895938.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24856..25242
                     /locus_tag="ACG8N9_000303"
     CDS             24856..25242
                     /locus_tag="ACG8N9_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895939.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25229..25927
                     /locus_tag="ACG8N9_000304"
     CDS             25229..25927
                     /locus_tag="ACG8N9_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4895940.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25932..26396
                     /gene="lspA"
                     /locus_tag="ACG8N9_000305"
     CDS             25932..26396
                     /gene="lspA"
                     /locus_tag="ACG8N9_000305"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4895941.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26393..27304
                     /locus_tag="ACG8N9_000306"
     CDS             26393..27304
                     /locus_tag="ACG8N9_000306"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4895942.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27539..28078
                     /gene="pyrR"
                     /locus_tag="ACG8N9_000307"
     CDS             27539..28078
                     /gene="pyrR"
                     /locus_tag="ACG8N9_000307"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4895943.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28092..29375
                     /locus_tag="ACG8N9_000308"
     CDS             28092..29375
                     /locus_tag="ACG8N9_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4895944.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29388..30314
                     /locus_tag="ACG8N9_000309"
     CDS             29388..30314
                     /locus_tag="ACG8N9_000309"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4895945.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30333..31613
                     /locus_tag="ACG8N9_000310"
     CDS             30333..31613
                     /locus_tag="ACG8N9_000310"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4895946.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31618..32694
                     /locus_tag="ACG8N9_000311"
     CDS             31618..32694
                     /locus_tag="ACG8N9_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4895947.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32699..35884
                     /gene="carB"
                     /locus_tag="ACG8N9_000312"
     CDS             32699..35884
                     /gene="carB"
                     /locus_tag="ACG8N9_000312"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4895948.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35938..36714
                     /locus_tag="ACG8N9_000313"
     CDS             35938..36714
                     /locus_tag="ACG8N9_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4895949.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36711..37643
                     /locus_tag="ACG8N9_000314"
     CDS             36711..37643
                     /locus_tag="ACG8N9_000314"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4895950.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37640..38356
                     /gene="pyrF"
                     /locus_tag="ACG8N9_000315"
     CDS             37640..38356
                     /gene="pyrF"
                     /locus_tag="ACG8N9_000315"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4895951.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38363..38992
                     /gene="pyrE"
                     /locus_tag="ACG8N9_000316"
     CDS             38363..38992
                     /gene="pyrE"
                     /locus_tag="ACG8N9_000316"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4895952.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38980..39294
                     /locus_tag="ACG8N9_000317"
     CDS             38980..39294
                     /locus_tag="ACG8N9_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4895953.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39332..39973
                     /locus_tag="ACG8N9_000318"
     CDS             39332..39973
                     /locus_tag="ACG8N9_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4895954.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40016..40708
                     /locus_tag="ACG8N9_000319"
     CDS             40016..40708
                     /locus_tag="ACG8N9_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4895955.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40705..41607
                     /locus_tag="ACG8N9_000320"
     CDS             40705..41607
                     /locus_tag="ACG8N9_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4895956.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41765..43471)
                     /gene="efbA"
                     /locus_tag="ACG8N9_000321"
     CDS             complement(41765..43471)
                     /gene="efbA"
                     /locus_tag="ACG8N9_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4895957.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13975 a         7688 c         9483 g        12480 t
ORIGIN      
        1 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
       61 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
      121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
      181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
      241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
      301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
      361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
      421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
      481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
      541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
      601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
      661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
      721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
      781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
      841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
      901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
      961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
     1021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
     1081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
     1141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
     1201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
     1261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
     1321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
     1381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
     1441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
     1501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
     1561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
     1621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
     1681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
     1741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
     1801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
     1861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
     1921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
     1981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
     2041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
     2101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
     2161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
     2221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
     2281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
     2341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
     2401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
     2461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
     2521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
     2581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
     2641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
     2701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
     2761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
     2821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
     2881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
     2941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
     3001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
     3061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
     3121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
     3181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
     3241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
     3301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
     3361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
     3421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
     3481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
     3541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
     3601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
     3661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
     3721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
     3781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
     3841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
     3901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
     3961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
     4021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
     4081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
     4141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
     4201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
     4261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
     4321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
     4381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
     4441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
     4501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
     4561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
     4621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
     4681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
     4741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
     4801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
     4861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
     4921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
     4981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
     5041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
     5101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
     5161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
     5221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
     5281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
     5341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
     5401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
     5461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
     5521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
     5581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
     5641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
     5701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
     5761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
     5821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
     5881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
     5941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
     6001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
     6061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
     6121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
     6181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
     6241 aatcaatctt ctacaaaaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
     6301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
     6361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
     6421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
     6481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
     6541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
     6601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
     6661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
     6721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
     6781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
     6841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
     6901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
     6961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
     7021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
     7081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
     7141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
     7201 ttatcgttta ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
     7261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
     7321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
     7381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
     7441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
     7501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
     7561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
     7621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
     7681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
     7741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
     7801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
     7861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
     7921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
     7981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
     8041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
     8101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
     8161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
     8221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
     8281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
     8341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
     8401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
     8461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
     8521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
     8581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
     8641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
     8701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
     8761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
     8821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
     8881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
     8941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
     9001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
     9061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
     9121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
     9181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
     9241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
     9301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
     9361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
     9421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
     9481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
     9541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
     9601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
     9661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
     9721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
     9781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
     9841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
     9901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
     9961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    10021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    10081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    10141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    10201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    10261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    10321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    10381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    10441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    10501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    10561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    10621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    10681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    10741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    10801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    10861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    10921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    10981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    11041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    11101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    11161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    11221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    11281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    11341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    11401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    11461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    11521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    11581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    11641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    11701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    11761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    11821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    11881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    11941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    12001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    12061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    12121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    12181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    12241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    12301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    12361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    12421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    12481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    12541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    12601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    12661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    12721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    12781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    12841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    12901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    12961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    13021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    13081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    13141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    13201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    13261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    13321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    13381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    13441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    13501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    13561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    13621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    13681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    13741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    13801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    13861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    13921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    13981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    14041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    14101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    14161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    14221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    14281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    14341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    14401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    14461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    14521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    14581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    14641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    14701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    14761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    14821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    14881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    14941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    15001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    15061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    15121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    15181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    15241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    15301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    15361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    15421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    15481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    15541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    15601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    15661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    15721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    15781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    15841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    15901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    15961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    16021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    16081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    16141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    16201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    16261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    16321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    16381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    16441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    16501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    16561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaattaa gtacgaaaag
    16621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    16681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    16741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    16801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    16861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    16921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    16981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    17041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    17101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    17161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    17221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    17281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    17341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    17401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    17461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    17521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    17581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    17641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    17701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    17761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    17821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    17881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    17941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    18001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    18061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    18121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    18181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    18241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    18301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    18361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    18421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    18481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    18541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    18601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    18661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    18721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    18781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    18841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    18901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    18961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    19021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    19081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    19141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    19201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    19261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    19321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    19381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    19441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    19501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    19561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    19621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    19681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    19741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    19801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    19861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    19921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    19981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    20041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    20101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    20161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    20221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    20281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    20341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    20401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    20461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    20521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    20581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    20641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    20701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    20761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    20821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    20881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    20941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    21001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    21061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    21121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    21181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    21241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    21301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    21361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    21421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    21481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    21541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    21601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    21661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    21721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    21781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    21841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    21901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    21961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    22021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    22081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    22141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    22201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    22261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    22321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    22381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    22441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    22501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    22561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    22621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    22681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    22741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    22801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    22861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    22921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    22981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    23041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    23101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    23161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    23221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    23281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    23341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    23401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    23461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    23521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    23581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    23641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    23701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    23761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    23821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    23881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    23941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    24001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    24061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    24121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    24181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    24241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    24301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    24361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    24421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    24481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    24541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    24601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    24661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    24721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    24781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    24841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    24901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    24961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    25021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    25081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    25141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    25201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    25261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    25321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    25381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    25441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    25501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    25561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    25621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    25681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    25741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    25801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    25861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    25921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    25981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    26041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    26101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    26161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    26221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    26281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    26341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    26401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    26461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    26521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    26581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    26641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    26701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    26761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    26821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    26881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    26941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    27001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    27061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    27121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    27181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    27241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    27301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    27361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    27421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    27481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    27541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    27601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    27661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    27721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    27781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    27841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    27901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    27961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    28021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    28081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    28141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    28201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    28261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    28321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    28381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    28441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    28501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    28561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    28621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    28681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    28741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    28801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    28861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    28921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    28981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    29041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    29101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    29161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    29221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    29281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    29341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    29401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    29461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    29521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    29581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    29641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    29701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    29761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    29821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    29881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    29941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    30001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    30061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    30121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    30181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    30241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    30301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    30361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    30421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    30481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    30541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    30601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    30661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    30721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    30781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    30841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    30901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    30961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    31021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    31081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    31141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    31201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    31261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    31321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    31381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    31441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    31501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    31561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    31621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    31681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    31741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    31801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    31861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    31921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    31981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    32041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    32101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    32161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    32221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    32281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    32341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    32401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    32461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    32521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    32581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    32641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    32701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    32761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    32821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    32881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    32941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    33001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    33061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    33121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    33181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    33241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    33301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    33361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    33421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    33481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    33541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    33601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    33661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    33721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    33781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    33841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    33901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    33961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    34021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    34081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    34141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    34201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    34261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    34321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    34381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    34441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    34501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    34561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    34621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    34681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    34741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    34801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    34861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    34921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    34981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    35041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    35101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    35161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    35221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    35281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    35341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    35401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    35461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    35521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    35581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    35641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    35701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    35761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    35821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    35881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    35941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    36001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    36061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    36121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    36181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    36241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    36301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    36361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    36421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    36481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    36541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    36601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    36661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    36721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    36781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    36841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    36901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    36961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    37021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    37081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    37141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    37201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    37261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    37321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    37381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    37441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    37501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    37561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    37621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    37681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    37741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    37801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    37861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    37921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    37981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    38041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    38101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    38161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    38221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    38281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    38341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    38401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    38461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    38521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    38581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    38641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    38701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    38761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    38821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    38881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    38941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    39001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    39061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    39121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    39181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    39241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    39301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    39361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    39421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    39481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    39541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    39601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    39661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    39721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    39781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    39841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    39901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    39961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    40021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    40081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    40141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    40201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    40261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    40321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    40381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    40441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    40501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    40561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    40621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    40681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    40741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    40801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    40861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    40921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    40981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    41041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    41101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    41161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    41221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    41281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    41341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    41401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    41461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    41521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    41581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    41641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    41701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    41761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    41821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    41881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    41941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    42001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    42061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    42121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    42181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    42241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    42301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    42361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    42421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    42481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    42541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    42601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    42661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    42721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    42781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    42841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    42901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    42961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    43021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    43081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    43141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    43201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    43261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    43321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    43381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    43441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    43501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    43561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    43621 ttaggt
//
LOCUS       DAZUKD010000007        42310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000007 DAZUKD010000000
VERSION     DAZUKD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.007"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..295
                     /gene="nrdH"
                     /locus_tag="ACG8N9_000322"
     CDS             71..295
                     /gene="nrdH"
                     /locus_tag="ACG8N9_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4895958.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            454..2613
                     /gene="nrdE"
                     /locus_tag="ACG8N9_000323"
     CDS             454..2613
                     /gene="nrdE"
                     /locus_tag="ACG8N9_000323"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4895959.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2663..3628
                     /gene="nrdF"
                     /locus_tag="ACG8N9_000324"
     CDS             2663..3628
                     /gene="nrdF"
                     /locus_tag="ACG8N9_000324"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4895960.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3717..4856)
                     /locus_tag="ACG8N9_000325"
     CDS             complement(3717..4856)
                     /locus_tag="ACG8N9_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4895961.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5165..5878)
                     /locus_tag="ACG8N9_000326"
     CDS             complement(5165..5878)
                     /locus_tag="ACG8N9_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4895962.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6132..6833)
                     /gene="nagB"
                     /locus_tag="ACG8N9_000327"
     CDS             complement(6132..6833)
                     /gene="nagB"
                     /locus_tag="ACG8N9_000327"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4895963.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7067..8599
                     /locus_tag="ACG8N9_000328"
     CDS             7067..8599
                     /locus_tag="ACG8N9_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4895964.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8790..9992)
                     /locus_tag="ACG8N9_000329"
     CDS             complement(8790..9992)
                     /locus_tag="ACG8N9_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4895965.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10074..10763)
                     /locus_tag="ACG8N9_000330"
     CDS             complement(10074..10763)
                     /locus_tag="ACG8N9_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4895966.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10869..11237)
                     /gene="rplL"
                     /locus_tag="ACG8N9_000331"
     CDS             complement(10869..11237)
                     /gene="rplL"
                     /locus_tag="ACG8N9_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4895967.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11290..11793)
                     /gene="rplJ"
                     /locus_tag="ACG8N9_000332"
     CDS             complement(11290..11793)
                     /gene="rplJ"
                     /locus_tag="ACG8N9_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4895968.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11997..12124)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12203..12892)
                     /gene="rplA"
                     /locus_tag="ACG8N9_000333"
     CDS             complement(12203..12892)
                     /gene="rplA"
                     /locus_tag="ACG8N9_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4895969.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12996..13418)
                     /gene="rplK"
                     /locus_tag="ACG8N9_000334"
     CDS             complement(12996..13418)
                     /gene="rplK"
                     /locus_tag="ACG8N9_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4895970.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13761..14429
                     /gene="sdaAB"
                     /locus_tag="ACG8N9_000335"
     CDS             13761..14429
                     /gene="sdaAB"
                     /locus_tag="ACG8N9_000335"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4895971.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14442..15314
                     /gene="sdaAA"
                     /locus_tag="ACG8N9_000336"
     CDS             14442..15314
                     /gene="sdaAA"
                     /locus_tag="ACG8N9_000336"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4895972.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15405..16505)
                     /locus_tag="ACG8N9_000337"
                     /pseudo
     CDS             complement(15405..16505)
                     /locus_tag="ACG8N9_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(16597..17484)
                     /locus_tag="ACG8N9_000338"
     CDS             complement(16597..17484)
                     /locus_tag="ACG8N9_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4895973.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17561..18106)
                     /gene="nusG"
                     /locus_tag="ACG8N9_000339"
     CDS             complement(17561..18106)
                     /gene="nusG"
                     /locus_tag="ACG8N9_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4895974.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18207..18377)
                     /gene="secE"
                     /locus_tag="ACG8N9_000340"
     CDS             complement(18207..18377)
                     /gene="secE"
                     /locus_tag="ACG8N9_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4895975.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18401..18553)
                     /gene="rpmG"
                     /locus_tag="ACG8N9_000341"
     CDS             complement(18401..18553)
                     /gene="rpmG"
                     /locus_tag="ACG8N9_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4895976.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18715..19359
                     /gene="cbpA"
                     /locus_tag="ACG8N9_000342"
     CDS             18715..19359
                     /gene="cbpA"
                     /locus_tag="ACG8N9_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4895977.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19415..19918)
                     /locus_tag="ACG8N9_000343"
     CDS             complement(19415..19918)
                     /locus_tag="ACG8N9_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4895978.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19931..19977)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20515..21480
                     /locus_tag="ACG8N9_000344"
     CDS             20515..21480
                     /locus_tag="ACG8N9_000344"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4895979.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21579..22751)
                     /locus_tag="ACG8N9_000345"
     CDS             complement(21579..22751)
                     /locus_tag="ACG8N9_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4895980.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22738..23883)
                     /locus_tag="ACG8N9_000346"
     CDS             complement(22738..23883)
                     /locus_tag="ACG8N9_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4895981.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(23897..24821)
                     /locus_tag="ACG8N9_000347"
                     /pseudo
     CDS             complement(23897..24821)
                     /locus_tag="ACG8N9_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            24938..26017
                     /locus_tag="ACG8N9_000348"
     CDS             24938..26017
                     /locus_tag="ACG8N9_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4895982.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            26014..26642
                     /locus_tag="ACG8N9_000349"
                     /pseudo
     CDS             26014..26642
                     /locus_tag="ACG8N9_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(26752..26979)
                     /locus_tag="ACG8N9_000350"
     CDS             complement(26752..26979)
                     /locus_tag="ACG8N9_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4895983.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(27082..29670)
                     /locus_tag="ACG8N9_000351"
     CDS             complement(27082..29670)
                     /locus_tag="ACG8N9_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4895984.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(29690..30349)
                     /locus_tag="ACG8N9_000352"
     CDS             complement(29690..30349)
                     /locus_tag="ACG8N9_000352"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4895985.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(30398..30901)
                     /gene="trmL"
                     /locus_tag="ACG8N9_000353"
     CDS             complement(30398..30901)
                     /gene="trmL"
                     /locus_tag="ACG8N9_000353"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4895986.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(31122..31988)
                     /locus_tag="ACG8N9_000354"
     CDS             complement(31122..31988)
                     /locus_tag="ACG8N9_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4895987.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            32097..32738
                     /locus_tag="ACG8N9_000355"
     CDS             32097..32738
                     /locus_tag="ACG8N9_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4895988.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(32800..34161)
                     /gene="mgtE"
                     /locus_tag="ACG8N9_000356"
     CDS             complement(32800..34161)
                     /gene="mgtE"
                     /locus_tag="ACG8N9_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4895989.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(34179..35078)
                     /locus_tag="ACG8N9_000357"
     CDS             complement(34179..35078)
                     /locus_tag="ACG8N9_000357"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4895990.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(35080..35877)
                     /locus_tag="ACG8N9_000358"
     CDS             complement(35080..35877)
                     /locus_tag="ACG8N9_000358"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4895991.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(35855..36559)
                     /locus_tag="ACG8N9_000359"
     CDS             complement(35855..36559)
                     /locus_tag="ACG8N9_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4895992.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            36690..37265
                     /locus_tag="ACG8N9_000360"
     CDS             36690..37265
                     /locus_tag="ACG8N9_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4895993.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            37383..38060
                     /locus_tag="ACG8N9_000361"
     CDS             37383..38060
                     /locus_tag="ACG8N9_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4895994.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(38139..39947)
                     /gene="pepF"
                     /locus_tag="ACG8N9_000362"
     CDS             complement(38139..39947)
                     /gene="pepF"
                     /locus_tag="ACG8N9_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4895995.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(40123..41121)
                     /locus_tag="ACG8N9_000363"
     CDS             complement(40123..41121)
                     /locus_tag="ACG8N9_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4895996.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(41347..42000)
                     /locus_tag="ACG8N9_000364"
     CDS             complement(41347..42000)
                     /locus_tag="ACG8N9_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4895997.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        12791 a         8803 c         7473 g        13243 t
ORIGIN      
        1 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
       61 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
      121 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
      181 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
      241 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
      301 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
      361 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
      421 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
      481 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
      541 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
      601 tttctatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
      661 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
      721 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
      781 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
      841 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
      901 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
      961 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     1021 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     1081 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     1141 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     1201 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     1261 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     1321 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     1381 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     1441 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     1501 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     1561 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     1621 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacgga tatctcatgt
     1681 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     1741 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     1801 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     1861 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     1921 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
     1981 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
     2041 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
     2101 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
     2161 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
     2221 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
     2281 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
     2341 gcttatgata tggacatgcg taaagtaatc gatgtttatg caatggcaca acaacacgtc
     2401 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
     2461 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
     2521 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
     2581 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
     2641 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
     2701 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
     2761 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
     2821 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
     2881 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
     2941 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
     3001 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
     3061 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
     3121 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
     3181 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
     3241 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
     3301 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
     3361 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
     3421 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
     3481 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
     3541 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
     3601 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
     3661 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
     3721 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
     3781 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
     3841 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
     3901 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
     3961 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
     4021 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
     4081 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
     4141 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
     4201 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
     4261 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
     4321 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
     4381 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
     4441 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
     4501 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
     4561 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
     4621 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
     4681 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaactgc cgaaagcatt
     4741 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
     4801 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
     4861 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
     4921 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
     4981 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
     5041 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
     5101 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
     5161 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
     5221 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
     5281 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
     5341 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
     5401 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
     5461 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
     5521 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
     5581 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
     5641 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
     5701 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
     5761 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
     5821 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
     5881 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
     5941 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
     6001 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
     6061 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
     6121 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
     6181 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
     6241 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
     6301 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
     6361 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
     6421 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
     6481 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
     6541 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
     6601 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
     6661 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
     6721 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
     6781 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
     6841 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
     6901 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
     6961 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
     7021 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
     7081 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
     7141 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
     7201 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
     7261 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
     7321 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
     7381 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
     7441 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
     7501 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
     7561 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
     7621 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
     7681 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
     7741 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
     7801 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
     7861 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
     7921 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
     7981 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
     8041 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
     8101 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
     8161 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
     8221 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
     8281 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
     8341 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
     8401 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
     8461 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
     8521 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
     8581 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
     8641 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
     8701 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
     8761 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
     8821 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
     8881 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
     8941 ccaataaagt acataaacat aaatacagta ttatttcggt tgcttgctgc ttcacctagc
     9001 ccttgtactc gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc taataaaata
     9061 atagctaaaa gcaaaatggc caaatggctt ccgccaacca ataaagcaat aaagcttatt
     9121 gtttgcataa caagtcccat atacacgatt tttctttcag aataagaatc tgccagtcgt
     9181 cccacgatag gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg
     9241 aatgttcccc aattgtaggc gggactgctg atataaaaaa tcaacgtaga ccaaaagatc
     9301 gaaaatgttc caaacatgaa aaatccactg actgccgctt ctcttaatag acgttgagaa
     9361 gcaactaaat gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt actactttgc
     9421 tggacagaac gcggcatttt ccagtaaagc agaagtgtaa gaattccgac tgcacataaa
     9481 gcaaataaat agatcatccg ccaagaaaaa agactggcaa caataccaga taccgtccga
     9541 gaaagcaaaa tccccacgag taaaccgcta agcaatgtac ccatgacctt ccctcttgct
     9601 tttggtcccg ctagtaccgc accgtaagga atgatgattt gcggcacgat cgaaagtaat
     9661 cctattaaaa aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac
     9721 agagagacgg cagataacgc tgccattcga atgatcaatt ttggacgatc caccacgtcc
     9781 cctaaaggaa ctaaaaacag taaccctaac gcatagccta attgggtcaa catcgtcaat
     9841 atcccgactg cacttgtgct aacagataaa ctagctgcta cttttgttcc aatcggctgg
     9901 atgtaataca tattggcaac aacaacgcca caagtgattg ataatagtaa aataagcgaa
     9961 gttgttaaag gttttttttc attactcttc attgatttcc cccatttcct atcttagttt
    10021 acaacttatg gaaaagattg gaaatagaaa aatacaacta ttcgccacac ttattatttg
    10081 tgcttttgtt ttccttgttt tttcttgcga tctttataaa tgaagacaac taaccctact
    10141 agcacagtaa cacttgcaac caacgtgact gcagttttgt tatatccgtg aaacagagct
    10201 tctccaccac acgcatacaa cacagacgaa gggatgactc caatcacgat cgacaatatg
    10261 atttgctgta gaggcaattt catcgcatct gcagcaaaat tgaccataga agatggaata
    10321 accggtatca tatagcctaa cattacacct atttttggat gtttcatttg tcggattgct
    10381 tgcacccaat gattcgtttc ggttttctta tctaaaaatt ttaagcgatg cataaggaaa
    10441 attgcagtga tgttaccaag agcattaccc aaaatgttga tgatcgcacc tgtcagtggt
    10501 ccaaagctca aaccagcaat gactccaact actgaagtag gaatacctgg aacagcacac
    10561 atcaccgctg tcaatacgat caagagtatc gatgcaaaag tcccatgcga acgaatatgt
    10621 gcgagtagct ctgccttact cgctctcgga tttaaaaata attggatgtc tgtctgatat
    10681 tctaaataca accgatagcc cactaccagt atcagaatga ttcccaacga gatcaatatc
    10741 aatcggatta ttttcttttt catatttcct cctgaaaaaa atctatattc attaaataat
    10801 agtttaacat aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat
    10861 agtaaaaatt atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct
    10921 tcagcttctt ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct
    10981 ttagcttctt tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt
    11041 tggtctccag ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg
    11101 ccaccagcag cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata
    11161 gctttaacta ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt
    11221 tcaatgttca atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt
    11281 atgcggcaat taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc
    11341 cacgttgcgg actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt
    11401 tggcaatttt gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc
    11461 gcctttgatt tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc
    11521 gactacatct tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc
    11581 ttcaaggcca gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc
    11641 tacgttagca tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg
    11701 gtagtcaacg actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat
    11761 tgctgctttt tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga
    11821 tggaagagtt ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt
    11881 attattcatc acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa
    11941 ctcctttcgc tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt
    12001 cacaataaaa aactctatgc caccgcagac atagagggac tattgaatat attcaataaa
    12061 cgtcctcggt aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat
    12121 taacctcaac tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat
    12181 caatcaacat tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa
    12241 atgttgttgt gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt
    12301 ttagcaatac atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa
    12361 cttttccgat tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag
    12421 ctttcacttc gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg
    12481 gcattaagcc ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag
    12541 gtgttgcaac aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt
    12601 cgtcacctac gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa
    12661 aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt
    12721 gttggtctgc ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa
    12781 attttgcaat gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac
    12841 ttgaatcaac tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc
    12901 ctccttgatt gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga
    12961 gccaactgag aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt
    13021 cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc
    13081 atttttaatt cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg
    13141 tttggttcgc ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc
    13201 ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg
    13261 atcaaacctg cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg
    13321 atacccgctt gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga
    13381 atttgcaatt taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg
    13441 tgatgtggtt aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat
    13501 ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt
    13561 ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttattctaa
    13621 aaaaacaaca atttttcgtt ctcatttctt cttttatgtt acacttgcgc tagcatggtt
    13681 caacagatga aagcaaacaa taacacggtt ttttagaccc ttgtacaaag attcctacat
    13741 aacaaaatga ggtggagaaa atgaaagatt taaggtatag aagtgtcttt gatattattg
    13801 gacctgtgat gattggtcca agtagctctc acactgccgg agctgctaga atcgggaaaa
    13861 tcatacgtag tatatttggc gaacagcctg attctgtaga tatttatcta tatgaatcat
    13921 ttgcaaaaac ctatcggggg cacggaacag atattgcatt agttggcggc ctgttaggga
    13981 tggaacccga tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg
    14041 aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc aaactctgta aagatgcttc
    14101 taaaaaaagg aacacgaaag ctttcagtca ctggcatttc gatcggcggt ggcaatatcc
    14161 agatttctga attgaatgga ttcaaaattt ctttaagcat gggtactcct accttgatcg
    14221 ttgtccacca agatgttcca ggtatgattg ctaaagtgac tagtatactt tctgagacca
    14281 atacgaatat cggcactatg acagtgacac gggaatcaaa aggtgaaaat gccattatga
    14341 taattgaagt agatgatcct caagtagaag aaacggtaaa aagactcaaa cagctgccaa
    14401 atatcgacag cgtaaattat ttcgattaag gagctaaaaa aatgttttta tcgattgaag
    14461 aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt gcagttgaaa
    14521 tggaaatgag tggccgttcc cgtagtcaca tcatcgagat catggaaaaa aacctctcag
    14581 taatggaaca atcaatcaac gagggaaaag aaggtgttac ctccgtcaca ggaattaccg
    14641 gtggtgatgc aaaaaaatta gatgattatt taaatgaggc agacttttta agtggagaaa
    14701 caatcttaaa agctgtacgc aatgctgtag ctgttaatga agtcaatgca aaaatggggt
    14761 tgatttgtgc tacaccgact gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg
    14821 tccgagatcg gcttgatttg acacatgaac agcaattgga attcttattt accgctggcg
    14881 ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag
    14941 cagaagttgg ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca aaaaatggga
    15001 ctcctcatca agcagcacaa gcagtagcca ttacattgaa aaatatgatg ggactgattt
    15061 gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt
    15121 ctcaagcttt tatctctgct gatatggcat tagcgggtat cgaaagtgtc attccgccgg
    15181 atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa
    15241 cagcagaagg tggcttagct gttacgccaa ctgctaaacg acttgcggaa gagattttaa
    15301 ataagagggc gtgataaagc tccttaattt cgagtattaa gagattagtt atcttcataa
    15361 caaaaaacga agagaggact aattgccctt ctcttcgttt tttattaatc catcaatttc
    15421 gtatttttta cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct
    15481 ttgttcaggt gttcaaaaac agatgttact ccttctgccc cacctagatt caacccataa
    15541 atcactggtc ggccaatcgc aactaagtca gcaccacttg ccagagcttt gaagacatgc
    15601 tctcctcgac ggacaccaga gtcgaaaatc actggtacac gtttgttgac tacttcggca
    15661 atttttggca atacttcaaa ggacgcagga cctccatcta gttgacgtcc accatgattg
    15721 gatacccaga taccatctgc ccctgcagag atagcgatta ctgcatcttc tggcgattgt
    15781 attcctttta cgataactgg aagatgggtg atttctttga ttgttttgat gtcatctggt
    15841 gtcaagcctt gttttgcagc tgcataaatt tctgcgattc cttttccttc gccattacca
    15901 cttgcacttt gttcgctata cgcagctaag ttaggcattg gtaatggaaa ttggaattga
    15961 ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa aatgatagct
    16021 ttagcccctg cttctactgc tttgttcaaa atgaactcat tgaatctgtc gtctttactc
    16081 atatataatt ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc
    16141 gtatttgcat aagtgctgat ggaaaagatt gatccagcag cagctactcc tttagctgta
    16201 tcagcttccc ctttttcatg tgccaatcct tgggccgctg atggcgcttg gataatcggt
    16261 gtatctaatt caattccgaa aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat
    16321 atccgtggca tgattttctt tgtattaaaa gaagtcgtat tttctttcat cgtccactca
    16381 tcttcagaac caccgcgaat atagccaaaa gctcctgctt ccatgcgctc ttttacacga
    16441 ccttctagtt aggcaatgtt tacaatatcg attggatgtt cttcattact tgtatgataa
    16501 gtcatagtag atccttcttt catcttcttg attacatata aatcatacta aacttacaaa
    16561 aagtaagcaa acatttactc tcagatactt ttatcgctat tctttccaaa taaatcgact
    16621 tacaagctga tctacttctt ggttttcatg aagcatacta tgagaggcat tttcacctgt
    16681 cactactttt tcctctattt catttccgcg ttgcttcaac aacgcataga ccgctaatgc
    16741 actgtttaac ggtactgttc catcactcaa gtctgtttca tctagttgac cagcaatcaa
    16801 gaaaaatcga gtggttatag gaatattacc gatcatttgc tggtaatctt gatagcggtt
    16861 agaaacgaca gcaggcccct ttgctaactc atctgtcaga gactgcgcac tatcatcgac
    16921 aaaatcatta aaaggcgcgc caattgctgt taatgtctga acttgtggca atgacgtatc
    16981 ctgtccatac gttcctaaat agcgtagtgc actaactcct cccatggaat gccccactat
    17041 gttgacttta tctatgccgt agttttcttt cagatacaaa agacaagttt tgatccattc
    17101 tgcttgattc cactcgttat ttttattatc ttcaaacaat acttggacca tcggattgtc
    17161 tgcttttcca gacaaatctc ctgttgcttg tatttgtcca tctgttccta ctgttaaaac
    17221 caattctttt actgcttgct gatttttttc caaacgccgc aacatccctt taaaggaatt
    17281 ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt ctatcacttt
    17341 actggattct gccgtttttt ctttatcaga aacttcagta gtatttatct tcgtcgtctg
    17401 gcaaccactt aacgcaaaca aactgataag gactaaagct gctgcagttg cacgtttgca
    17461 taaatttttg attggttctg tcatacacct cttcctttcc atatagaaaa aagaaagact
    17521 tgaaaggcca atcatatgat cgacatttca agtctcttta ttaatcgatt ttatctactt
    17581 gatcgaaatc aagttctgta ctggtttcac gtccaaacat atcgatgttg actttcaatt
    17641 tttctttttc tccgtcaact tctgttactt gtccttctaa tccagcaaat gcgccttcga
    17701 tgattttcac gacttcgcca acttctactt ctagatcatt ttgtcgcagg ctcataccga
    17761 tagaacgcaa gatatgattg acttcttctg gaagtaatgg tgctggtttg cttccggctc
    17821 cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg gacaacatac catgaagcgt
    17881 ctgtcatgat catttcaaca agtacgtatc ctgggaatgt tttatgaacg atttcttttt
    17941 ctttcccatt tttgacttca gtttcttttt cttcaggaac aacgacacgg aaaataaagt
    18001 cggccatttt catactttgc gcacgagatt cgatatttgc ttttactttg ttttcatatc
    18061 ctgaatatgt atgcaagaca taccagtttt tttcaaaaga ttccattgaa ggctcctcat
    18121 ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt ttctttcgct tcattatagc
    18181 actaaaagta cgttgtggct agcgagttat tttaaaatcc agccaaataa gctttggatg
    18241 gctgtatcca ttacgtaaaa catcaatccg aaaataatag aagtttcgat gactactaac
    18301 gtatctttac ggagttgttt tctgcttggc caagttactt gtttgatttc atcttttacg
    18361 ctttttagaa atttcatgag acaacctccc ttgctactgt ttatttcgtt tctttatgta
    18421 atgtatattg attacaatat ttacaaaact tattgatttc caaacgttcg ccgctttttc
    18481 cttcactgac tgattttgtg tagttgcgag acccacaaac ggaacaagcg agtgctgctt
    18541 ttttctttgc catttacttc tgcccccttg ctgagaatca gacttatact tcataacagt
    18601 atcacgtaac tttttacttg tcaatttgtg aaataaacta tctattgttt tggataaaat
    18661 tatgatatga ttaatttgaa attaagctaa aatgagacaa ttggaggtat agcgatgcta
    18721 ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta
    18781 gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct
    18841 tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat
    18901 ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac
    18961 ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc
    19021 cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc
    19081 tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag
    19141 caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt
    19201 attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct
    19261 aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa
    19321 gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa
    19381 cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg
    19441 cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg
    19501 caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa
    19561 tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt
    19621 tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa
    19681 aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa
    19741 ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa
    19801 actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca
    19861 tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt
    19921 tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg
    19981 aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa
    20041 aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt
    20101 aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag
    20161 aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta
    20221 gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc
    20281 cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac
    20341 ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt
    20401 tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa
    20461 ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa
    20521 ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca
    20581 gaaagaattt taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa
    20641 tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg
    20701 atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac
    20761 ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct
    20821 tatattccag caggttctgg agatgatttt gctcgagggg caggccttcc aaaaaatccc
    20881 aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct
    20941 tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta
    21001 gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac
    21061 aacttaggat cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga
    21121 tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt
    21181 acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg
    21241 tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg
    21301 ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca
    21361 acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa
    21421 atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa
    21481 taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt
    21541 gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt
    21601 tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca
    21661 aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg
    21721 agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct
    21781 attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa
    21841 ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa
    21901 gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga
    21961 taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag
    22021 ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt
    22081 cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaatgctaga
    22141 ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag
    22201 gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct
    22261 ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct
    22321 tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta
    22381 gaaaaggcat ctgggcgact ctgtatcttg atcgtcggca tctttcctac ttcgacttgt
    22441 tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca
    22501 tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat
    22561 ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct
    22621 tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg
    22681 ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta
    22741 taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat
    22801 aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag
    22861 tttatacaaa ctttcgctga tcaagttgac gagatttaat tgaccaacaa tcggaatatg
    22921 aagatcaatc caatacttca cttgttccga ccccatcata ccagcaaaaa agctcatcgc
    22981 aatcgtcact gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt
    23041 accaaccaat gtacctaaca aaatcgcaca caacgacccc agagcggtaa caatcatcaa
    23101 ccattgccaa cgttcaccaa agtctacttg atagataaaa cgaaagacag ctaacataat
    23161 ataaatctct gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg aaactaacga
    23221 ttgctttcta ggaatcaagg acaagcgaat accatttgac gattgatttg cttgctgatc
    23281 ctgtacattg cggacccccc acatcgtccc gtagagaatc gccattccaa tcagagtgaa
    23341 gaaataaaaa cttttgaaac tcatattacg acctgcatga acttcttgaa aagatacttc
    23401 ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc tccacttctg ccactttact
    23461 cgaaattata ccaatacgct gtaaaaattg atccatgacg tttttcatca acgtctgact
    23521 cattcctgct tgacccacat ggagcgaaac agatgaacca tcaatgagat aaaaaccagc
    23581 caaatcatct gcatttagct tttcattcgc ttctacttcc gatagttcct gtacttcaaa
    23641 taatggttta tcattgactt cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc
    23701 tgtttgatcc gctttgatta caattccagt attaatcgtt tcgagattgc caccttgatc
    23761 aatctcgcca aaggccgcca taaacatcaa tcctaaaaga attggaaagg ccaatgtcca
    23821 aaacaacatg gatttatttc gcagcagtat ttttgtgcga tatttataaa aatgccaaaa
    23881 cattatttcc cctcctctag tctctcaaat ccttgccagt aatctctaaa aagacatcat
    23941 tcaaggacgc ttctttggtt gctatttttc cgtaactgat cttttcttct tgtaagattg
    24001 taagaatcgg caacaatact gtatttagtt cagtagttgt tacttggagc tgaccttttt
    24061 tataagcgat tccatcagaa gctattgttc gctgacaaca cgttttctgg gagtaatggg
    24121 atgtcgatcg tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct
    24181 ttagcaatca cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct
    24241 tccatgtaat gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt
    24301 tctaaaattt tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt
    24361 aaccgcggct tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta
    24421 agttctcctg gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca
    24481 atcaaggctt tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt
    24541 tcttgcacac ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc
    24601 ttgacagcat aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca
    24661 taatttaaca aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggtcct
    24721 aacagaccta agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa
    24781 tctccatagc gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa
    24841 atttcttgtt gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa
    24901 accacacatg acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa
    24961 cgaaactagc tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc
    25021 tatgcaagtt atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc
    25081 tacaaagcca aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga
    25141 tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga
    25201 ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa
    25261 ggtcgccttt gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg
    25321 aacgaccgat cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca
    25381 agagttatcg gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca
    25441 aattgtggaa gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct
    25501 gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa
    25561 agaaccatta gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag
    25621 tgtccatgac ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc
    25681 tgattttcct tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca
    25741 gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca
    25801 tgccaaaacc gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac
    25861 gaatgatggc accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg
    25921 tcaacgtgtt caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca
    25981 gttaacgatc attttaccaa gggagggaca ccaatgataa cagtagtcat tattgagatg
    26041 atccatttgt gacgaaatcg ttacaaacca ttttagaaag cacgaaagag atccgtgtgt
    26101 taggcattgg tcattgtgcc aaggatgcgc tagtcttata cgaaacccat cgtcccgatg
    26161 ttttgctgac ggacatccgc atgcctaaac aaacgggcat cgatgctgcc aaagaaattt
    26221 tggctaaatt tcccaaagcc atccttttgc ttttgacgac attcaaagat gaagaataca
    26281 tccgcgaagc attttccatc ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga
    26341 ttattccttc tgtcaaatcc gcttataatg gacaggccgt atttggtaat gaaatcatcg
    26401 agactttcac tcaaatgatg aagaataaac cgacgattga tagtcatgct tatgcttctt
    26461 ttagtgaacg tgagcttgca atcatcaaag aagtagctgc cgggaaaaac aataaagaaa
    26521 ttgcagatgc cttgtacttg agtgacggca ctgttcgcaa ttacatcagc caattactag
    26581 aaaagctaga tttacgggat cgcacgcaat tggctatcta tttttatcag catttgcaat
    26641 aagtcttatc taacacaaaa aaacctgaat gatgctcctt cgcatcattc aggttttttt
    26701 cacatcttct attcaattgt cccgcaaacc atttcctcac gattgaaaga atcaaggatt
    26761 cgcagggctg ccaggattcg ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt
    26821 gtctaacgtt ttttctagtt ttgattcttc acgatcgtct cgttctacat caggactttc
    26881 attcaatttg aattcttttt ccatctcatc taacttttta aagtcattgc cagattcttt
    26941 tgctaatttc gctaatttat cttcggtttc tttagccata aagtaacgcc ccccttttga
    27001 tttcagctta gcatatcaca ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt
    27061 agctatttct catctttaaa atcaagcatt ttttgccggt ataaaatcgt agggagaact
    27121 gttttccata ccaatcatcg gatctactaa ctggttatcg atcacttctg ctgtaagaac
    27181 gaaatcttta gaaacagcag gttctacctc ttctttcttt tcttctccaa caaatgaaga
    27241 aaacagatct ggttccactg tttccattac ttggtttttc gtttcattcg tcccactgtc
    27301 atcttctaca ggtagctttt cttttacaac ccgtggctct tttgtatagt tcccatcttc
    27361 aaatggatca tttccgacca aagaatcttc ataagctgct agtttggtcc ggagagtgat
    27421 attcatttta tcattatcca tgatccttga tttcaacgta gtcatccggc ttgctgattc
    27481 attcgctaca caagtgacat aggcttgctc ttcccctaac aaaatcaaca gttccttact
    27541 gcgagtcact gccgtgtaga gcaaattacg ctgaagcatc cgctgatatt gatggaccat
    27601 gggcaaaata accatcttga attcactgcc ttgtgattta tggattgagc aacaataaga
    27661 aagagtgatt ttattccatt cgtttctttt ataagtgacc tcatttgcat caaactggat
    27721 aactaattca tccactttat cttcagaatc ttttgccaag atgatacccg tgatttctcc
    27781 catatctcca ttgaatacat tcaattcggg agtattgact aattggagga ctttgtctcc
    27841 tatccgataa acggtgtcgt tccaatgtac ttcctttttt ccaggtttcc ctggattgaa
    27901 tatctcctgc atcattttat ttaacgcatc gatccctgca ggtccccgat acattggtgc
    27961 taatacttgg atatcttgtg cacggaatcc cttatttttc gctttttcca caattttaga
    28021 gatcaagggt tcaatttgat aagcatcact tcggaaatag gaacgatctt tttgattctt
    28081 tgtgaaatct tttggcagac gtccttcttt gatctcatgg gcaagaggaa taatactaga
    28141 accatcacct tgccgataaa tctgattgag ttccattttg ggaatttgtt cgatttccaa
    28201 aagatcatgg agtacttgac caggaccgac agagggaagc tgatctttgt ctcctactaa
    28261 gataacttgc atattcgtcg gaatcgcttt gaataatgtg ttagccagcc atgtatcgac
    28321 catagacatc tcatcaacaa tcaaaagtcc gccttctaat tctttcgtac tgactgaagg
    28381 tgttttttcc cgtccattca gccccagaag acgatggatc gtactggcag gcagacctgt
    28441 cgtttcgttc attcgtttag ctgctcggcc agttggtgct gcaagtagaa taggaaaaat
    28501 ttcttgggta tattttgaag gatccaattc cagtccgttc aactctgcaa aaagattgac
    28561 gattccgttg atcacagtag ttttccccgt acctggtcct cctgttaaaa taaatagagg
    28621 ggaacggatt gcttcagcga tagcttgttc ttgcgaaggt ccatactgta tgccgaatat
    28681 tttttcgata cgccgaatat tcttctgcac ttctttttca ggataatgga tttctttttt
    28741 acgactgaga aggcgctgga tcgaattacc gatcccccat tcagaaaagt acaaactgtt
    28801 ttcgtaaaga ttagtctctt cttgttgtat ctttccttct tcaacaagtt ggataattac
    28861 agtagcaacc tgatcaggct ggatttcaat tgggcgactt gtttctaaaa tacggatcgt
    28921 ttgctgaagc aatgtctccg ccggtacgta tgtatctcct gtttgaactg catgctgaaa
    28981 aatctgatgc aagatagctg ctcgaattct tctcggtgaa tctgctgcaa taccgatttg
    29041 ttcggctaga ttatctgcac gtttgaaccc gattccttct atgtcttcga ctagctggta
    29101 cggattttct tcgatgatat ccaaagactc gttcttatag gcctggtaga tactgaaagc
    29161 taactgacta ccgaacccgt aacgattcag tcccatgatc acttgatcca tcccgtggtt
    29221 tagccgaatc gtctcagcaa gcatttctct ttttggcttc gtcaaaccgg agatttgttt
    29281 taataccgaa ggatcagcaa tgattttttc gatagcatct tctcccagca attctacaat
    29341 tttttctgct gttttcttcc cgattcctgg gaatttttcg ctggaaagat acgctacgat
    29401 cccatttgca gttgttggct tttcttgctc atatctttcg agtttaagct gccttccgta
    29461 tttaggatga tccactaatt ctccataaaa acgataagtc tcattttctt gaatatcgcc
    29521 gaaacttccg gtgactacaa tctctttttc acggtaatca gctgtggttt cactgatgtg
    29581 taccaaaagc actttgtaaa aattactagg attttgaaaa aaaatcgcgg caactgtacc
    29641 aacgaaataa ggcatttctt ctggctgcat cggaagccgc ctccttcttc tatagtaaag
    29701 gttctggtgc tgtttcagtt cctaaaaaat ctgtttggtt ccatactttc agttcatatg
    29761 gcaataatct gcaaggctct tcagtctcta aaatcgtcaa actactattg aacaatccac
    29821 ccatttcacg caactcactt agctcttttc ctgtcatcca ttgaatggct gcagttaaag
    29881 aagctccatg tcctacgaat aaaagtgggc catctttatg ttgggcaacc gcatctgcta
    29941 caacagtttc gatacgtgtg attgccgatt caatcgtttc tccgtgaaaa ggagaaggat
    30001 catacagatc taaatgatta cgcaaattcg gcagattatc cggatatttc tggtacattt
    30061 catcgatcga ctgaccttct aaagtaccca gccctagttc tcttagctta tctgtataaa
    30121 aaatctctac cggacgagca agctctttgt tgataccttc tgctgtatca cgtgctcgct
    30181 ttgctgtact ggaataaatt gctttaaaag gaacatattt tacctttttc ccaaatgctt
    30241 tgatttcttc gtaactttgt ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc
    30301 tttcctgatt ccattgtgtc ttcccatgtc gggtaaaata tagttccata cgcagcaaac
    30361 tcctgtcttt cttttatttt actctataat aaactgtcta atccagcttg tcattttcat
    30421 agtgatgcgt caattctaaa ttagggaatc cttgctggcg aagtgcttca tacactatca
    30481 ttgcagctgt attcgataaa ttcaatgagc ggacatgctc atcattcata gggatgcgta
    30541 aacacttttc ttcgttttca cgcataaatg tttctggcaa accggttgtt tcttttccaa
    30601 ataaaaagaa gtgctcttct ccatcagcaa aattttcgtc actatatact ttatgggcaa
    30661 attttgagat caaatgtagt ttacgattac ctaaaaatgt taaaaaagct tctaaattac
    30721 tgtggtaagt aatatttaca tcattccaat aatccaaccc tgcccgtttt agatgtttgt
    30781 catcagttga aaaacctagt ggctcgatta aatgaagcgg agtattcgtg gctgcacacg
    30841 tacgggcaat atttccagtg tttgcaggga ttaatggttc aaataaaacg atatgattca
    30901 tcaggatcat tcctctcata tttatgttcg tttctatttt ctgacaattt gatttttgca
    30961 taaaaaaaaa cgtatcaact cggtgtcagt cactgcgagt cgttacgtta gtgaagctct
    31021 tctcactaac ttttatcaga acaggaacct gataaaaacc aatgaaaaaa caacttgttg
    31081 ctaatataac accctcaact tttttttgca acctctactc attaaaaact gctagaaaaa
    31141 cgttttctct tagttccttt taataattct atgtcaattg taatcttctt caaaggattc
    31201 ttatataagt ctgctttgat tcgctcttct acctgccatt ctaaaggtga catggccaaa
    31261 agatcttttt gattttcctc tggtatctca aattcatagg ttacacgctg acgttcagtt
    31321 aatttcattg cttcagcaaa cttggctact acacgttcat tggaataact ctgtttttca
    31381 ggttgattcg gataaaaagc ctgacgtagt tcctttaaat aattttcttg tggcaccacc
    31441 ttcaacactt ctccatcttc tgagagtaca cgttggaatt cttggtaatg agaaggcgaa
    31501 aagatattta aaaccgcgtt gaatgtttga tcagcaaacg gcaaattggt caaatcagct
    31561 acacaccaaa atgcttcggc agattgttct gtagctgcgt agactccttc ttttgaaata
    31621 tcaaatccaa cagcattttg aatctttttc aattttgaaa gttcattcaa gaagctacct
    31681 tctccgcagc caacatctaa tagcgagact tcttcaggta aatgctgcgc aatagtctca
    31741 ataacgggct gatacatgcc agattggatc attctacgcc ttggaacgaa cattccttga
    31801 tcgtattctg ttttaaccgc atgggataaa aaataaagtg ttcctttttt ggaaaggtca
    31861 aaacggtgat tatttttgca tatcaagcca ttatcagact tttttacttc ttcttggcaa
    31921 accggacagc ggaacagctg atgattttct gccaaaaact ggcgtcctcg gtcaattttt
    31981 tttaacatag ttccccctca tttctgtttt atcttaccgt tagatgctca ctttggcaac
    32041 tgctaaaaat atgatacaat gcctagaaga aagtgaaaga taaaggagaa gattagatga
    32101 tcaaagaatt ttgcgctgaa aactacacat ctattcctct agcaattgct agaggagccg
    32161 atcggatcga attgtgtgac aacttagcgg tcggtggcac aaccccgagt actggtgtga
    32221 tcgaagaaac gttaagttat gctggggaaa aagatatccc cgtaatgacg attattcgtc
    32281 ctagaggggg cgacttcgtc tataacgata tcgaattgaa aatcatgcat actgatttga
    32341 ttgaagcaaa gaaattaggg acggatggcg tagtatttgg ttgtcttact ccttcaggct
    32401 ggctagacga agaggcctta gaactattga tcgaaacagc agaagggttg cagatcacct
    32461 tccatatggc ttttgacagt attcctgaag atcggcaatt cgaagcaatc gattggctgg
    32521 tagaacatgg tgttgaccgt atcttgactc atggcggtcc tgctgacaaa actattgtag
    32581 aaaatttccc acggcttaaa gaattgattg cctatacaga tggccgctta atcattttgc
    32641 ctggtggtgg tgtcactgca gagaatgcag cacatgtagc taaagaattg aacgtaagcg
    32701 aagtacacgg aaccaaaatc gtggatcttc agttataatc ttataaacaa aaaaacattt
    32761 gcaacaggca taatgcttat tgcaaatgtt tttgtttttt tacataaaat agcccataaa
    32821 taaagaagca atattgaaat aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa
    32881 gggaccgctt gctactgctg gatcaaatcc tagacgatcc attagcatcg ggattaaact
    32941 acctgcaaga ttcgccactg tgattgcaaa tagcatagcc atccctatga caaaccccaa
    33001 gataaaatta tgtttccaaa aacctacgat tagaaaaatc gtgatccccg taacagctcc
    33061 tgtgacaacg ccggtcaaga cttcagccaa aacaagtcga acaaatccat gatctttctc
    33121 atcagaaatc gcaagtcgac gaacagccac agctagcgac tgggttccag cattaccggc
    33181 agttcctgta atcagcgaaa tgaagactgc taaaatactc gcttcactta ccaagtcttc
    33241 atagtgactg attaaagaag cggtcgacat acctaaaaac aacagagtga tcagccaagg
    33301 caaacgtcga gacgctgcct ttactggatt ttcattaatt tcttccacat tgaccccggc
    33361 aagtcctgaa taatcgctag cagcttcatc atcaataacg tcgatgatat catcgactgt
    33421 gacgattcct aacaaatgat catcataatc ggtaacagga agagctaaga agtcataatc
    33481 tctaaatgtt tgtgccacat cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc
    33541 gctcatcaga tcagaaatca ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat
    33601 aacccctact agctgacctt cttgattaac gacatagatg taatagattg tttcagctac
    33661 atctgcttcg ttcttcagca cgtacatcgc agagcgaaca gtttgattgg ctacgatcga
    33721 aacaaattca gttgtcatga tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt
    33781 gatttcactg gcatcgtctg ccgacatcaa acttaaatat ttagcaattt gctttttatc
    33841 cagcgtatta agcaagtcaa cagcattatc tgtatacatc tcagaaagca tatcagctgc
    33901 atagctagga cgcatttcat ctaggtattc tttcatatgt tcgttgtctt cttcaatcac
    33961 atcgaacata tccgctaatt ctttaggcga cagataggaa tagatcagct gcctgttctc
    34021 ttctgtaaga gattggtaga attgcccctg ctcatagata tgcatctcta aaaaactgtt
    34081 tctgaatgcc tgaatattat tttcccttaa atcttgagtc aactgggcaa atctttcctc
    34141 taattcttga ttttcatcca aaccaatctc acctcgcctt attcttcaaa ccgtctcaca
    34201 actgcctcca tgtctgcggc taaaggctgc ttgaactcca gcaactcttc tgtaaatgga
    34261 tggtaaaaac gcagatcata acaatgaagg gcttgtcttt ttattcccag atccattcgc
    34321 ccgccataca tatcatcacc gagcagggca cagccaattg cggaaaaatg aactcttatt
    34381 tgatgtgtcc ttcctgtatg cagctggata tcaactaacg cgatttctgt tagtcgcttc
    34441 tctatccagt actcagtctg tgccgaacgt ccttcatcag agaccattcg cttcaatagc
    34501 gaagaataat ctcgggcgat cggcaaatcg atatgtccat gtgccgataa atctgaaagc
    34561 cttccactga ctaaggcctg atattttttc acaaacttct tttcacgaag ttgcatatct
    34621 aattttgcgt gcgcaaatcc atgctttgca aaaagcatca aaccagatgt atcccgatct
    34681 agtcgcgtca ccacatggat tacttggttt tcgtaacctt gttttttata ataagccttt
    34741 acacgattag ccattgtccc atttggatgg tattgggcag gaatagacga aacgcctgct
    34801 ggtttattta ctaccagaac gtgttcatct tcatacacga tatcgatcgg tgtttcatcc
    34861 aaaagtacgg tttcatgttc tccttcagca ggaatcacaa tggttacctt atcatcctta
    34921 gctagagaaa acaagacatt ttctacttga tcattaactt tgatttgacc gccttgaaat
    34981 tttattttcg ctaacagacc ctttgagatc cctttttctt ttaaaaaata ttttacttgc
    35041 tgagaatgat ctttctcata atcccatgta aactgcatat caatcctcac taataaaggc
    35101 atctttcaca cgatgccaaa aatgcatatg tcgataagaa gcaaaatgga tccgttcttc
    35161 tgctattcga taataaattg agcggatatt tgcttttgcg acattcaatt gatcaaccgt
    35221 tacaagataa tcttctgtat tttccaattt tatttctagc cattcatcat gggcaatcac
    35281 gatcggagaa cccaaagtac gaaacactcg attattcagt gaagcaatct ctgccaactg
    35341 aaaggcattg atactaggat ggatcacagc accgccgaca cttttattat atgctgtcga
    35401 accagtaggc gtagaaatcg tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt
    35461 gatgtataca tcggctacca tcgtacgatt ggcccgttta atcgttgatt cattcagtgc
    35521 taaaaaatgc tggtccggtg tttcatccaa ataactgatc cgaacatcca gcaagggata
    35581 actaacactt tgttcccggt tcgttcgtaa gctgtctact agttcttcca gctcgtaatc
    35641 ccgccaatcc gtgtaaaacc ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt
    35701 cagacaatga ctgtagtgat ggaacgccga caataatgtg ccgtctccgc cgactgagat
    35761 gaccaattct ggctcatgat catctcgttg aatccctgct tgttctaaaa gtgcgttcaa
    35821 acgttctgtc acttctatag tctttgcttc tttattatga acgatcgcta ctttcatccc
    35881 ctgcctcctc tctttcgctt tctttcttca aaagcgttcg atagtagaca tcatctgatt
    35941 ttcctcttcc atgggagaat aaccgctgtg cttcttggat ttcttcacgg atactggaca
    36001 tctcttcatc caactgatag gcagcttctg ccgcacgctg aagtcgttca ctcatttctt
    36061 ctggaaaaac gccttgatat ttataattca gcgaatgctc aatcgttgcc cagaaattca
    36121 tcgcaagagt ccgaatctgg atttccgcca aaattttctt ctcaccgctg atcagctgta
    36181 caggatactc gatcaccaga tgataagaac ggtagccact tgctttttta tttgtgatgt
    36241 aatctcgttc ttggagaact ttcatgtctt ttcttttacg gatgatctca acaacttgat
    36301 ggatatcttc tacaaactga cacatgatcc ttaacccggc aatatcagac atttcttctt
    36361 ctaaacgtcc cattggaata tgccgtaatt gtgattttgt tacaatacta tcgactggct
    36421 tgactctccc agtgacaaat tcaatcggga catgtcgatt ttgttcacgg aactgtttgc
    36481 ggatcccccg cagtttcact ttcaattctg atacagtttg ttcatatggt gctaaaaatg
    36541 cttcccaatc tcgttccata gctgatacct ctacttcatt aacattcatt gtttattcta
    36601 ccatatatgc aaagtttttt cgtactcttg atttatgcat tgaccaaaaa aagaaaatta
    36661 ggcaaaatag acaaaaagga gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat
    36721 ctatgctgac aaagcaagag tacgagatat tcatcgctca ctatcaatta acagataaag
    36781 atttccgtgt acagactaat atttatttcg ataccgttga tgaacagctg aagaaacaaa
    36841 attgtggcct acggatccgt ttcgttggtc atcaagctga atatacatta aaaacacctg
    36901 ctgaaaaagg gcgtctcgaa acaacggata cctttataac agaagaagca gaagcattta
    36961 tcttcgaaaa acgtctgcct cgttcaggcg cagtttttca aaaactttct gatttgaata
    37021 tcgatcctgc ttcattaatc caaaccggaa agcttacaac aaaacgggct gaattcccta
    37081 ttgaagaagg acttttagct attgatgaaa gctggggaga caatctacac gattttgaac
    37141 tggaactaga ggtaggggat gcatctgtgg gaaaaattgc ttttataaat tttttaaaac
    37201 gtttttcttt accttatcgt cctgctaaga acaagatcca acgtatgctt gaagctaaaa
    37261 attagaaacc tttcattttc acaaggcact tgagttcatt tttttacttt tttctgttaa
    37321 gttataggat gtgaaagaat ctagtcacta gttgtataga atctattttg ggggagattc
    37381 atatgatcga aatctattta tttgttaatc ctttagggga atactgtttc gaaatggaac
    37441 aacagttatt acaatttatc gaagaagagt acgggacacc atcaaaagaa aagatgcaat
    37501 ttcgctttct tcctttggtc aatttgcaga caattgggga tgtcatgcaa caaaaaggaa
    37561 tttctcagaa caatcttgag gaacgtaatc acttattctc aatgacttat tctgcagcat
    37621 tggactgcaa agcggcgcag tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat
    37681 tgcaagaagc agtcggctgt aatgacatac catattctca agaacttgct gaatccatat
    37741 ttgaagaaat cggcggcgat ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta
    37801 aagaagtatt cttttctgat caaaaaattg ctcgggaaat gggcatcaca aaacaccctt
    37861 ctgcagtcgt ttacaattat gcctgcgacc gtgattttgg tgttttagta gaagtaaaag
    37921 gtacaagtag cctttggaga atcacagagc tgtgtaaaac aacagataac tcatatcaag
    37981 tttttaatgg tgaacattta catacaaatg attctcagtt ccattcaaga actagccatt
    38041 tacacttgct ttctaattaa aaaaatacgt cgactccctt acaagagagc cgacgtattt
    38101 ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt ataaacgatc gatcaattct
    38161 tctaattcat ttagacgttg ttcaaacatc gacattgcat cttcaatata tgcagcctgt
    38221 gtcatatcaa ctcctgcttt tttcattact tccacaggat aatcgctgtt acctgctttt
    38281 aggtaagcca agtagttctc tagtgcctct ggttcctggt ttagtatttt cttcgccagt
    38341 gctgaagcag cagaaaagcc agtcgagtat tggaaaacat agtaattata ataaaaatgc
    38401 ggtatccgtg accattcgaa tttaatttct gggtcttctt cgactgctgg accataatat
    38461 tttgcattca atttaccata actatcactt aggtattcac tagtcaatgg cacacctttt
    38521 tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt
    38581 cctttaaacc catccaagta atggttgagt acataagccc gtacacgagg gtctttttct
    38641 gtttccaata aatactccgt taggatattt tcattcgttg tcgaagcaat ttctgctaaa
    38701 aagatggagt agtcgccgta cacataaggc tggttcgaac gagtgaaata actatgaaca
    38761 ctatgtccca tttcgtggac aagcgtaaat agctgatcca gcgtatcatg ccaattcaat
    38821 aaaatatatg gatttgtgtc atagcttccc gaagaataag catcgcttcg tttcccttta
    38881 ttttcgacaa catcgatcca acgttcagag aatgcttttt ctacgatggc catgtattct
    38941 tcacccattg gtttcagtgc ttctaaagct ttttcttttg cttcttcgta cgtaaaggta
    39001 attggagctt cccctaagac cggtgtataa agatcataca tgtgcagttt ttccacttct
    39061 aataaacgtt tccgtaattc catgtatcga tgtaacaaag gcaaatgctt gtttaccacg
    39121 tctaccaaag tatcgtatac actttcagga atatgattat tgctcaaaga cgcttctctg
    39181 gcagagctgt aattacggac tttcgcttta aaattatgtc cttttatatg tgtgcctaaa
    39241 gtagaagcaa atgtatttct aaattgttcg taaacactgt acaatccctt aaatgctgct
    39301 tcgcgcaccc ttcgatccgt gctttctagc aactgaccat acacgccatg agataattgg
    39361 actatttcac cattttctcc ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca
    39421 gcaaatgtat cacttgaagc atcaaagatt tcacctgctc cagcaagaag agattcctgc
    39481 tcagcagata agacatgggc tcgattatct actatttgct ggatataatg gcgatagact
    39541 tccaattttg gttcttcttt gaaatattgc caaatctggt catctgacaa ttgcaatatt
    39601 tctggttcaa accaggaaac ggcttcacta actttcgaaa acagactgct tgctcttgca
    39661 taaagcgctt ggtaatctgt atttccagtg tcttgatcgt ttttaaggtg cgcatataca
    39721 taaatgactt cagtttggcg ataaaccctc aatacgaatt caatcgcatt taaaaattga
    39781 gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt gttttaactc ttctgataat
    39841 tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt
    39901 aaattttcag gcaattcttc tcgttttggc aactgcttta cttccatgaa atccactcct
    39961 tttcttccct tagctctatc tatcaaaata gatcatagtc taacatccac atgtcacagc
    40021 atctaacttt tctactcatt catgtgtctg cttccatcaa ttttagcata actaaatctt
    40081 gttttacata gaaaaccgat tcctagccta tttttatact tactattttt tcaaagaaga
    40141 caagtgtatg cattcgttaa aaaactgctg atagaccatc tcttcatcaa tcataggaaa
    40201 aagccattcc cttttatgtt cttttatttg gcaaagccat gagttgaaat caggaacttt
    40261 ctggttttcc aaggcttctt cgtacaacat tcgataaaga aaaacctgtt cttgtaaata
    40321 aaagaaaaac cgactttttt gatagatcca cgatgataga tgcaaaagat gtttatgacg
    40381 caagtagcat tgttcttgta tggatactgt attcttttgc ttgtgaataa gttggcgtgt
    40441 cagccatttc ttcccatctt cactcacttt ttctattttt ttcatttggt tcattttaat
    40501 ttcgttacta gtgaatattt ctttaaggtc cctactagaa aaagtccagc agctactgca
    40561 aaaggaaata tgccctgata cagtctctgt aatatgatga tacaaataaa gcttttgttt
    40621 cttgagatca gctttccagc aatgcactcc tctttttcta ttatagtagc agcaactttt
    40681 ttctatcaat gagaattgcg attgtccaag aaaagaatta ccaagtatcc accatacttg
    40741 gtaactgtta ttcaaataat tttgggttct ttcaataaat ctctggtgag acagtctaga
    40801 acattggatt tctattgcta gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg
    40861 caaataggct tccaaaaata ctggttctac tgactgtccc agccaatcca tgaagcattc
    40921 ctttaactgt aagtgctctt ttgattctct ttcactaaag accgaacagt tatctgattt
    40981 ttgatgggca aaatgagcac attttttctt tccctttttc aaaatcactc tttttcgaca
    41041 gccacgacaa taatatttct ttcctttttc tgcatgttta gcaaaacagc acttattata
    41101 ttcattcatt gcaagcagca tttttctccc tctgaactta tttacgtcta agaatttatt
    41161 tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca agtaataaga acagctaaac
    41221 aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt accgcagatg caagcttttg
    41281 aacacattta tacgaaaaaa agactgcgac acaatttttg tcacagtctc ttactataat
    41341 gctgatttat acaaaataaa atttcgttcg ctcgatagca ttacgcgcca tcagacattg
    41401 tccatattca ttcaatgttt ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg
    41461 agcaatctga ttctctacat ccgcttggtt gagattttcc atccagaagt gaacgactag
    41521 gtaatagcgc tcttcgaata aatacaaatc agtccaagca gactctagtt cgacatctgt
    41581 tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc
    41641 ttctagggcg cttccagata attcttcctc cggatcgctg ccaataatct gctgacgaag
    41701 aaagtcactc acatcttctg aatcaacagt agaatcttct gattgagaaa tttcttcatc
    41761 cggaagattt ttactgataa acagttctaa tccgtctcct tttggcaaaa cttggaaagt
    41821 gacagcttcg cttcctcgga actcctcttc gatatcaact tcttccaaaa tactataaaa
    41881 gaaattttcg atttctttgt gattgccaag taaatccaaa aatgaaacgc cccgttcctc
    41941 taaatcttca tgaccaatta gtacacggat cgtattttca ttaatatgtt ccatttccat
    42001 tttgctacac ctcactttgc cacaatgttt aatatagatt cattgtagcg aaatcagctt
    42061 aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa taaaaccagc ggatttttca
    42121 acctttcaag ctatcttatt taactataac atttttggca gacgaatact atatagccga
    42181 cttttcagct aattttccct ctctattttt cgtgcaatca tcatcttcta ctaccttttt
    42241 atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt
    42301 aatagtcttt
//
LOCUS       DAZUKD010000008        41129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000008 DAZUKD010000000
VERSION     DAZUKD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.008"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..369
                     /locus_tag="ACG8N9_000365"
     CDS             142..369
                     /locus_tag="ACG8N9_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4895998.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            385..1551
                     /gene="hemW"
                     /locus_tag="ACG8N9_000366"
     CDS             385..1551
                     /gene="hemW"
                     /locus_tag="ACG8N9_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4895999.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKYGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1786..2829
                     /gene="hrcA"
                     /locus_tag="ACG8N9_000367"
     CDS             1786..2829
                     /gene="hrcA"
                     /locus_tag="ACG8N9_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4896000.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2923..3486
                     /gene="grpE"
                     /locus_tag="ACG8N9_000368"
     CDS             2923..3486
                     /gene="grpE"
                     /locus_tag="ACG8N9_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4896001.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3537..5366
                     /gene="dnaK"
                     /locus_tag="ACG8N9_000369"
     CDS             3537..5366
                     /gene="dnaK"
                     /locus_tag="ACG8N9_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4896002.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5517..6683
                     /gene="dnaJ"
                     /locus_tag="ACG8N9_000370"
     CDS             5517..6683
                     /gene="dnaJ"
                     /locus_tag="ACG8N9_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4896003.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7051..7788)
                     /locus_tag="ACG8N9_000371"
     CDS             complement(7051..7788)
                     /locus_tag="ACG8N9_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4896004.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7790..9292)
                     /locus_tag="ACG8N9_000372"
     CDS             complement(7790..9292)
                     /locus_tag="ACG8N9_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4896005.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9551..11545
                     /gene="uvrB"
                     /locus_tag="ACG8N9_000373"
     CDS             9551..11545
                     /gene="uvrB"
                     /locus_tag="ACG8N9_000373"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4896006.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11555..14371
                     /gene="uvrA"
                     /locus_tag="ACG8N9_000374"
     CDS             11555..14371
                     /gene="uvrA"
                     /locus_tag="ACG8N9_000374"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4896007.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14634..15899
                     /locus_tag="ACG8N9_000375"
     CDS             14634..15899
                     /locus_tag="ACG8N9_000375"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4896008.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15903..16634
                     /locus_tag="ACG8N9_000376"
     CDS             15903..16634
                     /locus_tag="ACG8N9_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4896009.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            16769..17653
                     /gene="rapZ"
                     /locus_tag="ACG8N9_000377"
     CDS             16769..17653
                     /gene="rapZ"
                     /locus_tag="ACG8N9_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4896010.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17650..18648
                     /gene="yvcK"
                     /locus_tag="ACG8N9_000378"
     CDS             17650..18648
                     /gene="yvcK"
                     /locus_tag="ACG8N9_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4896011.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18682..19617
                     /gene="whiA"
                     /locus_tag="ACG8N9_000379"
     CDS             18682..19617
                     /gene="whiA"
                     /locus_tag="ACG8N9_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4896012.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20185..20841
                     /locus_tag="ACG8N9_000380"
     CDS             20185..20841
                     /locus_tag="ACG8N9_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896013.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21020..21585
                     /locus_tag="ACG8N9_000381"
                     /pseudo
     CDS             21020..21585
                     /locus_tag="ACG8N9_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22284..22805)
                     /locus_tag="ACG8N9_000382"
     CDS             complement(22284..22805)
                     /locus_tag="ACG8N9_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4896014.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            23003..26683
                     /gene="nifJ"
                     /locus_tag="ACG8N9_000383"
     CDS             23003..26683
                     /gene="nifJ"
                     /locus_tag="ACG8N9_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4896015.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            26872..27759
                     /gene="cdaA"
                     /locus_tag="ACG8N9_000384"
     CDS             26872..27759
                     /gene="cdaA"
                     /locus_tag="ACG8N9_000384"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4896016.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            27756..28925
                     /locus_tag="ACG8N9_000385"
     CDS             27756..28925
                     /locus_tag="ACG8N9_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4896017.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            28949..30304
                     /gene="glmM"
                     /locus_tag="ACG8N9_000386"
     CDS             28949..30304
                     /gene="glmM"
                     /locus_tag="ACG8N9_000386"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4896018.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(30376..32181)
                     /gene="glmS"
                     /locus_tag="ACG8N9_000387"
     CDS             complement(30376..32181)
                     /gene="glmS"
                     /locus_tag="ACG8N9_000387"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4896019.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERYSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            32591..32881
                     /locus_tag="ACG8N9_000388"
     CDS             32591..32881
                     /locus_tag="ACG8N9_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896020.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            32894..33919
                     /gene="sppA"
                     /locus_tag="ACG8N9_000389"
     CDS             32894..33919
                     /gene="sppA"
                     /locus_tag="ACG8N9_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4896021.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            33922..34581
                     /locus_tag="ACG8N9_000390"
     CDS             33922..34581
                     /locus_tag="ACG8N9_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4896022.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            34738..35670
                     /locus_tag="ACG8N9_000391"
     CDS             34738..35670
                     /locus_tag="ACG8N9_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896023.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            35824..37113
                     /gene="tig"
                     /locus_tag="ACG8N9_000392"
     CDS             35824..37113
                     /gene="tig"
                     /locus_tag="ACG8N9_000392"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4896024.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            37360..38610
                     /gene="clpX"
                     /locus_tag="ACG8N9_000393"
     CDS             37360..38610
                     /gene="clpX"
                     /locus_tag="ACG8N9_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4896025.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            38693..39280
                     /gene="yihA"
                     /locus_tag="ACG8N9_000394"
     CDS             38693..39280
                     /gene="yihA"
                     /locus_tag="ACG8N9_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4896026.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(39354..39509)
                     /locus_tag="ACG8N9_000395"
     CDS             complement(39354..39509)
                     /locus_tag="ACG8N9_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4896027.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            39645..40973
                     /locus_tag="ACG8N9_000396"
     CDS             39645..40973
                     /locus_tag="ACG8N9_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896028.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        13523 a         7110 c         8954 g        11542 t
ORIGIN      
        1 gaagaatcat gagtgtaaaa aaatcatcca agaagtcgat tcttggattt tttagtggat
       61 ttgttcgcca aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc
      121 tgagcactta aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta
      181 atgccgaagg tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc
      241 tccgatgaca ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct
      301 tatttcaatg agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt
      361 tcgacgtaag gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt
      421 tctgcgtaca ttcatatccc attttgcgag catatctgct attactgtga ctttaataaa
      481 gtttttttag aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaga
      541 ttaatgctgg aaagtcatcc tacagcgaat ttggaaacac tttatgtagg aggcggaaca
      601 ccaacttctt tatctgctaa acaacttgat cgactattat caggtgctag agagatcctg
      661 ccttttcaaa atggtaatga gtttactgta gaagcgaatc ctggtgattt gacccgtgaa
      721 aaacttcaag tgatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt
      781 gacaatcgct tattgaaaaa aattggacgt aaacatactg cagaagatgt gtatcaaaca
      841 atgcaatttt tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta
      901 ccgggacaaa cactagaagg atatcgggat actttggaaa aagctttggc tttggatctg
      961 cctcactatt ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta
     1021 cgacaaggaa gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg
     1081 attgaagcaa tggaaaagta tggacgtcac caatatgaaa taagtaactt tgggctctcc
     1141 ggacatgaat ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga
     1201 gctggtgcta gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac
     1261 tatttacgcc cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga
     1321 aaaaatcaaa tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa
     1381 aaacatttct atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg
     1441 gaattagagc aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga
     1501 gggacttttt tggggaacaa tgtgtttgag cgctttttat ttgaaaaata gtttgaagaa
     1561 tatttgtttt ttatcgaaac ttgaaaagaa aaagtttaga aagtttgaca atgaggtatt
     1621 gaagtattga tataactgga tttacatcaa ttatttagaa ttagcactct tgtgtttaga
     1681 gtgctaaaaa atggagtctt tctcttgaca ggagaagggc ttcttgctat atttataagt
     1741 gaattagcac ttagaatata tgagtgctaa tgagaggtga ggaagatgtt aacaaagcga
     1801 caaaacgaca ttttacgtct gatcatccaa cactatacga gtagtggcgt gcccgttggt
     1861 tccaaaacat taatgtctga gggagtcaag gccagctcag caacgattcg caatgatatg
     1921 aaagcattag aagatgaagg attgctgttg aagactcact cttcttctgg gcggattcca
     1981 tctgctttag gctatcgtta ttacgtggat catttgttaa aacctgctcg cgtagcagaa
     2041 gatgatttgc tgctgattcg tcagtctcta ggcagagaat ttcatgaaat caatgagatt
     2101 attaagcaat ctgctgagat attatctgag ctgacaagct atacgacttt ttcattggga
     2161 cctgaaataa aagaccgccg actgactggc tttcgtatcg tacccttgaa taatcgacag
     2221 attattgcta tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt cactcttccc
     2281 gaatatatgg gaagtcagga actagaaaaa atggtacaga ttgtaaacga ccgtctagtc
     2341 ggagatcctc tgatgacggt gtaccataaa cttcgtacgg agattccgat gattcttcat
     2401 aaatatttcc agacgacaga agggatgtct cagttatttg acaccgttct tggtcatgtt
     2461 tttgaagaaa aggtttatgt cagtggacaa atgaatctct tggattatga gccccgtcaa
     2521 gatcttgatc agttcaaatc gatgttttct tttatgaaag actcagacgg acttacacaa
     2581 atgatcactc cgatggatag caatattcat atcaagattg gtgctgaatt aggcaatgat
     2641 ttattgcaga atatgagtat gattcaagca agttacgaga tagatggtca tggaagaggt
     2701 acaatagcct tgttagggcc tactagtatg ccttattctc ggatgttcgg tctagtcgat
     2761 gtttatcgaa gagaactagc agctaaactt gctgattact accgctcgct tgacttgtca
     2821 aactcgtaat gtgaataacg aagctatttg cagctgatcg ttctctcttt cttagtaaga
     2881 gcaatcaaac agctgactga ataatgaaag gaagactgta tcgtgaaaga aaatcaagaa
     2941 gaactagata aagaaatgac agatgctcag ccagaaccgg aaatcgatgt cgaagctgca
     3001 gaagattcgg gtatttcaga agctgaagcg gaagaattcg aaacagcgaa attaaaagct
     3061 gagttagaag aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc aaatatggca
     3121 aatcgaggaa aaaatgagcg tgagcaatta caaaaatacc gttcacaaga tttagccaaa
     3181 aaattacttc catcaattga caacctggaa cgtgcattag ccacagaagt gtcagatgat
     3241 caaggggcag gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg taacgcactg
     3301 gaagaagaag gaattgaaaa aatccctgct aaaggagaag cctttgatcc taatcttcat
     3361 caagcagtac aaactgttcc agcgactgaa gatacaccag ccgatacgat tgtagaagta
     3421 ctacaagaag gctacaaatt acatgatcgc gtgttaagac caactatggt cattgttgca
     3481 caataattgt gcggatataa aacttacact ttcaattaaa gaaggagacg ttaattatga
     3541 gtaaaattat cggtattgac ttaggtacga ctaactctgc agtagcagta ttggaaggaa
     3601 acgaagcaaa aatcatcgct aacccagaag gaaaccgtac aacaccttct gttgtatcat
     3661 tcaaaaatgg agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg acaaacccaa
     3721 atacgatttc atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta gatgtagaag
     3781 gtaaatcata tacaccacaa gaaatctcag caatgatcct tcaatatatc aaaggattcg
     3841 cagaagacta tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca gcttacttta
     3901 atgatgcaca acgtcaagca acaaaagatg ctggtaagat cgctggtcta gaagtagaac
     3961 gtatcgtcaa tgaaccaact gcagctgctc ttgcctatgg tttagataaa acagatcgcg
     4021 acgaaaaaat cttagtattc gaccttggtg gcggtacttt tgacgtatct atccttgaac
     4081 taggagacgg tgtctttgat gtattgtcaa cagctggcga caaccatcta ggtggggacg
     4141 actttgataa caaaatcatc gactacatgg tagcagaatt caaaaaagaa aacagcattg
     4201 acttgtctca agacaaaatg gctttacaac gcttgaaaga cgctgctgaa aaagcgaaaa
     4261 aagatttatc tggcgtatca agcacacaaa tcagcttgcc atttatcaca gcaggagaat
     4321 ctggaccatt gcatttggaa atgacattga cacgtgcaaa atttgatgaa ttaacagctg
     4381 acttagtaga acgtacaaaa gtaccagtac gtcaagcgtt aaaagatgca ggtctgtcac
     4441 aatcagaaat cgacgaagtt attttagtcg gtggatctac tcgtatccct gcagttgtag
     4501 aagcagtacg taaagaaaca ggaaaagaac caaacaaatc agttaaccct gatgaagtag
     4561 ttgctatggg tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa gatgttgttt
     4621 tacttgacgt tacaccattg tcattaggta tcgaaacaat gggtggagtg ttcactaaat
     4681 tgattgaccg caatacaacg attccaacaa gtaaatcaca agtattctca actgcggcag
     4741 ataatcaacc agcagtagac atccatgtat tacaaggtga acgcccaatg gctgctgaca
     4801 acaaaactct aggaagattc caattgacag atattccagc agctcctcgt ggtattccac
     4861 aaatcgaagt aacattcgat attgataaaa acgggattgt aaatgtatct gcgaaagatc
     4921 taggtacaca aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta acagacgaag
     4981 aaatcgaacg tatggtgaaa gatgcagaag caaacgctga agcagataaa gctcgtaaag
     5041 aggaagttga cctacgtaat gatgtagacg cattgctatt ctctgtggac aaaacattga
     5101 aagaattaga aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca gctcgtgacg
     5161 aattaaaagc cgctgttgaa gcaaacaaca tcgaggaaat gaaaactaaa cgcgatgcat
     5221 tgaacgaaat cgttcaaaac ttgactgtta aactatacga acaagctgca caacaacaag
     5281 ctcaagaaaa tccagaagca gctcaaggcg gagcagatga tgtcgttgat gctgattttg
     5341 aagaagtcga tggcgacgat aaataatcag ttcactgaat ttcgagagaa ggccaaagtt
     5401 gctactttgg ctttttcttt tgaaaaatag tttgaagtat cggtttttgt ttcaaacaaa
     5461 atatgatatg ataacaagcg acgtgaaaat gatgagaacc aataggagga aagcctatgg
     5521 caacaaaacg tgattattat gaagtcttag ggctctcaaa aggagcatca gaagatgaaa
     5581 taaagaaagc ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat aaagaacctg
     5641 atgcggaaga aaaattcaaa gaagtatcag aagcatatga gattttaagt gacccgcaaa
     5701 aacgcgctgc atacgaccaa tacgggcatg caggcacaga tccaaattac ggtgctggtg
     5761 gagctggtgg cggatttggt ggattcggcg gcttctctgg tggcggcttc ggtggttttg
     5821 aagatatctt cgactctttc tttggaggag gcggtcggtc agtcgatcca aatgcgcctc
     5881 gtcaaggtgc tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg atttttggtg
     5941 tagaaaaaga aatcaagtat aatcgggaag aaatctgtca tacttgtggt ggaaacggtg
     6001 caaaaccagg cacacaacca actacttgtc ataaatgtca tggtgcaggt acgatcaatg
     6061 tagaacgcca aacgccactt ggacgagtga tgagccgtca aacttgtgat gtttgtcatg
     6121 gcacagggaa agaaatcaaa gaaccttgtc caacctgtca tggctcagga catgagaaga
     6181 aagcacattc agttaaagtg aatgtaccag ctggtgtaga agaaggacaa caaatgcgtt
     6241 tagctggtca aggagaagct ggagaaaacg gcggaccatt tggtgatttg tatgtggttt
     6301 ttcgtgtaga agaaagtgat atctttgatc gtgaaggttc tgaaatttac tatgaattgc
     6361 ctttaagttt tgtacaagca gctttggggg atgaagtcca agtaccaact gtccatggag
     6421 atgtgaaatt gaagatccct gctggaacac agacaggtac gaacttccgt ttacgtggaa
     6481 aaggcgctcc gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa gtaaaactga
     6541 tcacaccgaa aaatctaaat gaacaacaaa gagaagcatt acgtgcattc gctgaagctg
     6601 gcggcatcaa agttgaagaa cagcaagaag acggtttctt cgataagttt aaagatgctt
     6661 ttggcgggaa aaagcggaag tagtttttct agtaaaaata taaagtagac ttgcccaaat
     6721 tttctatttc tagtaaaaac gctaagtcat taataattaa aaaccagacg aacaatcata
     6781 cgttcgcctg gtttttttgt agttgtcatg tttgtaccaa caaaaaagcc agctttagaa
     6841 taagataatc atcatagttt ctgaaggtgg tatcgactat gtacttgata tcgtcaatga
     6901 accgatggta ctttcttatg cgatggaaca ggtagagcga ttgcttgttt gtgcaggaga
     6961 acaagcgatg cggctggtac agttaagtga aaaaacaaga aactaaaaat gcccggtcgt
     7021 catgaagcga ccgggcattt ttggttttta ttaaatattt aatactttat tcaagaaatc
     7081 ttttgtccgt tcattttgcg gattttcaaa gatttgttga ggcgttccgt cttctaaaaa
     7141 gtttccgtcg tcaatgaaca tgacacgatt ggccacttct ttagcgaagc ccatttcgtg
     7201 cgtaacgata accatcgtca taccttgttc tgctaacttt ttcatgacgt tcaagacatc
     7261 accgaccatt tcggggtcga gtgcagatgt aggctcatca aaaagcatga tgtcagggtt
     7321 catcgctaga gcacgagcaa tagccacacg ctgcttttgt ccaccggaaa gcatttcagg
     7381 atacatatct tttttatctt ctagaccaac tgtctgcagc aatttgattg cttttttctc
     7441 tgcttcttct ctagattctt tcttcaggtc catcggtgct aaaatgatgt tttccaaaat
     7501 agaaagatga gggaataggt taaagtgctg gaataccatt ccgatatgtt gtctcaccaa
     7561 gttgatatct gtgcttttat ccatcaagtg ggcgccgtca ataataattt caccgtcaga
     7621 aggttcttct aaacgattca agcaacgcaa aaatgtactt tttcctgaac ctgaagggcc
     7681 aatgatacag acgacatctc cttcgttgat ggacacattg atgtcgttta atactgtgtt
     7741 atcgccgtat tttttgacta aatgctcaac taagattttt tctgccatct tacttcacct
     7801 tcttttctaa aacttttgct aattttgtca atgctgtgat caaaatcaag tacatgatcg
     7861 caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc aacaatgatc ttaccagttt
     7921 gcagcaattc gactacaccg attgcggaaa tgatcgttgt gtcttttaat gagataacga
     7981 actgattgac gaatgatggg atcatgatgc gtatcgcttg cggcaaaatg attttttgca
     8041 ttgtccgatt ataagaaaga cctaaacttc tggaagcttc catttgtccg acaggaacgg
     8101 cgttgatccc gcctcgaaca atctctgcga tataagcact tgcgttaaga gtcaaggtga
     8161 taacaccagc aaggaagtca ggaatcgtaa atccaacgat accaggcaac ccaaagaaaa
     8221 taaagaacgc aagaaccatc attggaattc ctcggatgat atcgacataa atactagcaa
     8281 ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc aaaaatcaca ccgattacta
     8341 tagccaaagc aaaggagatc aaagttagga gaattgttct ccaaagtcct ttcaacaatg
     8401 cttgatagtt gttttggata agtccaagaa atgttgattc atcagcagtg gaagactcac
     8461 tggaagtttc tgaaccatcc gctacatatt ctttaataat cttatcgtat tcgcctgttc
     8521 tttttaattc tttcaatcct tcattgaaca tattaagaag ctcaggattt tgtccttttt
     8581 taacggcaaa tgcatatttt ccgccagatt cttttttgat cggtgtttcc aattcttgtc
     8641 cttgtttgat cgcataaccg attaccggat aatcatccat cattgcatcg attgcgccaa
     8701 cttttaaccc gtcgtacaat tgatcagcag catcaaaata tttgatagaa tagccatatt
     8761 cagattcatg gtcgcttaaa aattcagcac tttcggtacc aacttttgct ccgactgttt
     8821 ttcctttcaa atcttcataa gacttgatcg aagtatctcc ttttttgaca gctaattgga
     8881 tcccgctatc aaagtaagaa tcagaaaaat catagctttt ctttcgttca tctgttacag
     8941 tcattccggc aatcattccg tctgcttgtc ctgattccaa agcttgcaaa gcagcagaga
     9001 aaccacggta gtccattcgg atgttaaatc cttgcagttt ggcgatgttt tccatcaatt
     9061 ttacatcgat gccttcgtac tcgccctttg cgttttgaaa ttcaaatgga gcaaaagcag
     9121 agtcactggc aattacgtat acgtcttttt tgggctctat tttttgcatt gtctcgcttg
     9181 tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac atgtccaaac gcaagtgtca
     9241 caagtcctgt cattaagaaa acaaatgagt gaaataaatg ctttcttttc ataatcaacc
     9301 tcctaaattt ccaatatttt attattatag gcgaaaagtg ctgaatcaga caagactgta
     9361 agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt atgttataaa acgtaacatg
     9421 cataataaga taaaaaagtg gcgaaagtat gttcggtgtg gtataatttt ttagtaagga
     9481 atgcctttgc tttccttttc tttttattgg tcgtaaaatg aaaaggaatg aatgaaaagg
     9541 aggacgaaga atgattgaga gagatacctc ccgacacttt gagcttgtct cgaaatacca
     9601 accggctggt gatcagcctg aagcgattaa ccaattagtg gatggcgtag tcggcgggaa
     9661 aaaagcacaa atcttattag gtgcgactgg aacagggaaa acatatacga tttccaatct
     9721 tattgagaaa gtgaataaac caacactaat tattgcccat aacaaaacat tggctggtca
     9781 gctgtacggg gaattcaagg aatttttccc taacaatgct gttgaatatt ttgtttctta
     9841 ttatgattat tatcagcctg aagcttatgt gccttcaagc gatacgtata tcgaaaaaga
     9901 ttcaagtgta aatgatgaga tcgacaaatt acgacattcg gctactagtt cattattaga
     9961 aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt tttggattag gttcgccttt
    10021 tgagtaccaa aaacaagtag tttcgattcg acagggagca gaactagatc gcaatcagtt
    10081 gattcgtgac ttagtcagta tccaattcga aagaaatgat atagattttc agcgcggtcg
    10141 ttttcgagtg cgcggtgatg tcgtggagat ttttccggct tcaagagatg aacgtgcctt
    10201 acgtgtggag ttttttggag atgaggtcga acgtatccga gaagtcaatg cactgacagg
    10261 tgaagtttta ggagaaacgg aacacgtggc aatcttcccg gcgactcact ttgtgacgaa
    10321 tgatgaacat atggagcatg ccgtagcgaa tatcaaagcg gaattagaac agcgtttaac
    10381 tgtcttgcga aatgaaaaca aacttctgga agctcaacgt ttagaacagc gaaccaatta
    10441 cgatattgaa atgatgttag aaatgggcta cacgtcggga attgaaaatt attctcggca
    10501 tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta ttggatttct ttcctgaaga
    10561 ttttttgatc gtggcggacg aatcgcacgt aaccatgcct caagtcagag gaatgtataa
    10621 tggtgaccga gcgagaaaac agatgctagt agattacggt ttccgtttgc catctgcttt
    10681 agacaatcgg ccacttcggt tagaagagtt tgaaaaacat gtcaatcaga tcatttatgt
    10741 ctctgcgacg ccaggaccat atgaacatga acaaacagat accgttatcc aacaaatcat
    10801 ccgtccgaca gggctgcttg acccagtgat cgaagttcgt ccaatcatgg gacaaatcga
    10861 tgatctagta ggcgaaataa atgaacgagt cgaaaaagac cagcgggtgt ttgtaacgac
    10921 attgacgaaa aaaatggcag aagacctcac agattatttc aaagaattag ggatcaaagt
    10981 caaatatttg catagtgata tcaaaacgct ggaacgaaca gaaatcattc gagatcttcg
    11041 attaggtgaa tttgatgtgt tagttggaat caatttgctt cgtgaaggat tagacgtacc
    11101 tgaagtttcc cttgttgcta tcttggatgc ggataaagaa ggctttttac gtagtgaacg
    11161 ttccttagtc caaacgattg gacgagctgc tcggaatgaa gaaggtaaag taatcatgta
    11221 cgcagacaaa gtaacagatt ctatgcgtct agcgatggat gaaacatcaa gacgccgtac
    11281 gattcagcag aagtacaatg aagaacatgg gatcgttcca aaaacgatca ttaaagaaat
    11341 ccgagatttg atttctatta cgaaagaatc ggaagatgat acaaaagaag cggtacaagt
    11401 ctcgtatgaa gaaatgacga aagaagaaaa agatacgtta ttgatgaaat tagaaaaaga
    11461 aatgaaaaat gcggcgaaag cattggattt tgaaacagcc gctaatgtgc gagatatgat
    11521 tttagaatta aaagcttcaa agtaggagga caacatggct aacgataaaa ttacgattca
    11581 cggtgcacgt gcccataact tgaaaaatat agatgtaacg attccacgag ataaattcgt
    11641 ggtcgttaca ggtttgtccg gttctggaaa aagttcccta gcatttgata cgctgtacgc
    11701 tgaagggcag cggagatacg tagagagcct ttctgcttat gctcgtcagt ttttaggaca
    11761 aatggataag ccggacgtag atagcatcga cggattgagt ccggcgattt ctattgatca
    11821 gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact gtgacggaaa tcaatgacta
    11881 tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt cccaacgatc atatcgagat
    11941 taccagtcaa tcagtagatc aaatggcaga tcaagtaatg gctttgcctg agcgcacaaa
    12001 gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga caacataaaa aaatattcga
    12061 acgaatccaa aaagagggat acgtccgcgt tcgagtcgat ggtgagatct atgatttaag
    12121 tgaagtgcct gagctggaga aaaacaaaaa acatgatatc gcgatcgtca tcgaccggat
    12181 cattgtgaaa gaagggattc gttctcgtct atttgattct ttagaggcgg ctctgcgatt
    12241 ggcagacggc tatgctctgg ttgacgtgat cggtgaagaa gaaatgttgt ttagcgaaca
    12301 ctatgcctgt ccttattgcg gatttactgt tggcgaacta gaaccaagac tgttctcatt
    12361 caatgcgcct tttggagctt gtccagactg tgatggttta ggtgtcaaat tagaagtcga
    12421 tcaagattta gtgattccag atagaacgaa gacacttagt caaggtgcta tcgttccatg
    12481 gaatccaatc agctctcagt attatcctca aatgttagcg caggcttgtg aaagtttcgg
    12541 tatcgattta gatacaccat tcgaagaatt accggaagag catcaggata tcatcttgaa
    12601 tgggacagat gatcttttcc atttccatta tgaaaatgat tttggtggcg ttcgcgatgt
    12661 agaaacgact ttcgaagggg tattgaagaa tatcgagcgt cgttatcatg atacgaacag
    12721 tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa ttaacttgtc agacatgtaa
    12781 aggttatcga ttgaatcccc aagctctatc tgttcaaatc aatggaacga atatcgggca
    12841 aacaaatgag ttgtctattc aaaaagcagt agatttcttc tcaaacttaa cgttatctga
    12901 acaagagaaa gtaattgcca aaccgatttt aaaagaagta aatgacagac tttcattctt
    12961 ggaaaatgtg ggactagact atctaacttt gagtcgcgct tccggaacat tatctggggg
    13021 agaagcgcag cgaatcagat tggctacaca aattggatcg aatctttctg gagttttgta
    13081 tattttagat gaaccatcga tcggattgca tcaacgcgac aacgatcgac tgattggctc
    13141 attaaagaaa atgcgtgatc ttggcaacac cctcgttgta gtcgaacatg atgaagatac
    13201 gatgtgggca gcagattatc tgattgacgt cggacctggg gccggagaac aaggcggaca
    13261 aatcgtggct gcaggcactc ctgaagaagt agctaatgat gaaaattctt tgacagggaa
    13321 atatctttca gggaaaaaat caatccctgt accaaaagaa cgccgtaaag gtaatgggaa
    13381 agcaatcaag atcactggtg cttcagaaaa caatctgaaa aatattagtg ttgaatttcc
    13441 attaggagaa tttgtagcag tcacaggtgt gtctggttct ggaaagagta cattggtcaa
    13501 tagtatctta aagaaatcgt tagcgcaaaa attaaataag aattctgcta agccaggtaa
    13561 attcaagaca atttccggct acgaaagtat cgaaaagatc atcgatatcg atcaaagccc
    13621 aatcggccgg acgccgagaa gtaatccagc gacttataca agtgtatttg atgatatccg
    13681 tgggttattt gctcaaacga acgaggcaaa aatgcggggt tataagaaag ggcgttttag
    13741 tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg gatggaatta ttaagatcga
    13801 aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta tgtcatggca aacgatataa
    13861 ctctgaaaca ttagaagtgc attacaaagg aaaaagcatt gctgatattt tggaaatgac
    13921 agtagaagat gctgtagaat tcttcaagca cattccaaag attcatcgca aactgcaaac
    13981 gattgttgat gttggcttag gttatgtgac gatggggcaa ccagcaacga cattgtccgg
    14041 tggtgaggca caacggatga aacttgccag tgaattgcac aaaatctcta atggaaagaa
    14101 tttctatata ctagatgaac caacgacagg acttcatagc gatgacatcg cccgcttgtt
    14161 gcatgtatta caaagattag tagatgctgg taacacagtt ttagtgattg aacacaatct
    14221 agatgtaatc aaaacagcag attatatcat tgatttagga ccagaaggtg gagaaggtgg
    14281 aggaacgatc cttgcgactg gaacaccaga agaaatcatt aacgtaaaag aaagttatac
    14341 aggtcactat ttgaaaaaaa taatggtata aagaggatag atagctgaga tagttaaaaa
    14401 aacataaaaa aatgaatttt tgcttgtttt attaatatca gtgttctaga atcattacat
    14461 ggtagttctt tctacactga ttggtataga caaaaacttc ctataagttc aatccgttca
    14521 aaaaagaatt gagcaatcgg aggagtttcc tttgattgct tttttcttta tggtatgata
    14581 ttccttgtgt agttggaaaa gaaaaacaag agattatgag taagtgagag ataatgatga
    14641 acagtattga aaatgaagaa tttatcccta tattattagg aagcgacatg aacgtttatg
    14701 gtatggcacg ttcttttaat gaagcatacg gcaaaatctg ccaagcatat gcttcggatc
    14761 agttagcccc tacccgctac agtaaaattg tcaatgtaga agttattcca ggatttgata
    14821 aagaccctgt gtttatcgag acgatgcttc gtttggcaaa agaaagatat tctgataaga
    14881 gtaaaaaata tcttttgatt gcttgtggtg atggatatgc agaattgatc tcccaacata
    14941 aacaagagct gtcagaatat tttatctgtc catatattga ttattcttta tttgaaagac
    15001 tgatcaataa agtaagcttt tatgaagttt gtgaagaata tgatcttccg tatcctaaaa
    15061 cattgatcgt cagagaagaa atgttagtaa atggacattt ggaacaggaa ttgccttttg
    15121 aattcccagt tgctttgaaa cctgcaaaca gtgtggagta tctttctgtc caatttgaag
    15181 gaagaaaaaa agcttttatt ttagagacta gagaagagtt cgatttaatt ttaggccgga
    15241 tatacgaagc cggttataaa agtgaaatga ttgtacaaga ttttatcccg ggtgatgata
    15301 gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca tcaagttcgg atgatgtgtc
    15361 ttggtcatcc actgttagaa gatcctacac cagcatcgat tggtaattac gttgtgatca
    15421 tgccagatta taacgaaaaa atctatcaaa ccattaaggc atttttggaa aaaatcgaat
    15481 atacaggctt tgccaatttc gatatgaagt atgatcctag agatggagaa tataaattat
    15541 ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt gacattgaac ggcttgaatc
    15601 tggctcgttt cgtcacagaa gatcgagtgt ttaacaaacc ctttgttgaa acgacctatg
    15661 gaacgaatca atcagataaa gcaagattat ggatgggtgt gcccaaaaaa atctttttag
    15721 aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca aatgatcaaa gaaaatcgtt
    15781 acggtactac tgtgttttat gaaaaagacc ggtcaatcaa gcgctggttg ttgatgaaat
    15841 atatgttcca taactatatt ccaagattca aaaagtattt tcatgtgaaa gaaggctagt
    15901 tcatggagaa ttttttcagt attttaggcg gaatgggcac gatggcgaca gaaagttttg
    15961 ttcgcttgat caaccaccga acgaaagcta caaaagatca agaatatttg aattatgtct
    16021 tattcaatca tgcaacagtt cctgatcgta cagcttatat tttagatcga tcagaagaaa
    16081 atccaatgcc gtttctgctg gatgatattg agaaacaaaa tttattgcag ccgaacttca
    16141 ttgtgttgac ttgtaacacc gcacactact tttttgaaga attacaagcg gcaacggata
    16201 ttcctatcct tcatatgcca agagaagcag caaatgaact tgtccgtcag catacgacag
    16261 ggagagtagc gattctagga acagaaggaa gtatgaaagc tggaatctat gaacgcgaag
    16321 tcaaaaatct tggattcgaa acagtgattc ctgatactgt tcttcaagaa aaaatcaatt
    16381 atctgatcta tcatgagatc aaggaatcgg attatttgaa tcaagagcta tattacgaaa
    16441 tcttagaaga agcggtagag cgcctaaact gtgaaaaagt tatcttaggt tgcacagaat
    16501 tgtcattgat gcatgaattt gctgaagata accattatcc agtgatcgat gcccaatcta
    16561 ttttagcaga tcggacgatt gaacgagcgc tagctgaaag aagcaaggca ttggatacag
    16621 catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc taaaagatat ttggtccaaa
    16681 tctttttgta aaagaaaaag aaaactggta atgatacgcc cggaatgata taattttact
    16741 atgcacaatg tgagaggagt tcgtaattat gacagataat ttgcaattag tcatcataac
    16801 aggtatgagc ggtgcaggga aaacagtagc tatccaaagt tttgaagata tgggctattt
    16861 ttgtatcgat aatatgccgc ctcgattgat tccaaaattc tgggaattaa tcaaagaatc
    16921 aggcaaagtc acaaaaatgg ctttaggagt ggatttacgt tctcgttcct tctttgaaga
    16981 aatccaaaac atgctgatcg aaatcgaaaa tacgaacttc atcgacactt ctatcttatt
    17041 tttagatgct tcagacgaag aactagtggc tcgctacaaa gaaacaagac gagcacatcc
    17101 aatggcgatg gacggtttga tcactgaagg gatacgtaaa gaacgggcga tactagacga
    17161 cttgaaagcc caagcttccg tgatcattga tacgacgaca ttgacaccac gacaattacg
    17221 tgaaaaaatc aatgagtcat tcaaatcaaa gggagaccaa ggatttcgga tcgaatttgt
    17281 atcatttggt tttaaatacg ggttgccgat cgattcggat attgtgatgg atgtgcgttt
    17341 tttgcccaac cctcattata tcccggaact aagaccattg accggaaaag atcagccagt
    17401 ctatgattat gtgatgtcct ttgatgaaac gcaatcattc taccataaat ttttggatct
    17461 tctggaaact gtattgcctg gatacattaa agaaggaaaa agcagcttga cagttgcaat
    17521 cggctgtaca ggcggtcaac accgttctgt ggcattgact gaacgtttgg caaatgcttt
    17581 agggaaaaac tataaagtca atgtgacaca tcgggacaag gataaaagga aagagacggt
    17641 gaaccggtcg tgaaaatgaa aacctaccgg ataagaaaac caaagatcgt agtggtcggc
    17701 ggtggcactg gtctgccagt tatcctgaaa agtttgcgaa atcaaggagc agatatcaca
    17761 gcagtagtga ctgttgcaga tgatggggga agcagcggtg caatcagaga atcgatcgct
    17821 atggctcctc ctggtgactt gagaaatgtc ttagtggcgt tatcggatat gccacaattc
    17881 tatgaagata ttttccaata ccgtttcaaa aaggaagatc agtttttagc caatcatacg
    17941 atcggtaatc tgatcattgc cgcagtttcc gagatgcggg gaagcactta tgaggcgatt
    18001 caattattag caaaaatgat gcatgtagat ggacatgttt atccttcttc agaaactcct
    18061 ttgaccttgc atgctgtctt taaagacgga acgactgcag taggagaatc taaaatcgcg
    18121 atcgaccgta aaaccattga ccgtgtgtat gtctgcaaca caaatgataa aagtgaagcc
    18181 aaagcggctc gaaaagtcgt ttcagcaatt atggacgccg acatggtcgt tttgggaccc
    18241 ggaagtttat tcaccagtat ccttccaaat ttagtgatac cagaaatcgg cgaagcaatg
    18301 ctcaagacaa ctgctgaaac cgtctatatc tgtaatatca tgacccaaaa aggggaaacg
    18361 gaacatttca cagatgccga tcatatacgt gttttacaca agcatttgaa tcagcctttt
    18421 atcgatacag tattggtcaa tacggaaaaa gtgcctgaag attatatgga cccagagatc
    18481 tatgatgagt atctagtaca agtcgcacac gacttcaacg gattgagaaa tgaaggatgt
    18541 cgagtgattt ccaccgactt tttggaattg aaagatggtg gtgtcttcca cgatggagac
    18601 aaagtagtag aagaattgtt ccgtttagtt ttcggttcaa aatattaagc aatttattgt
    18661 tggagaaagg aggagaatag gatgtcattt gctgcagatg tgaagaaaga actaactggt
    18721 ttggaagttc atcgagaaca tgcgaaagcg gaattagctg ctttgctgcg aatgaacggc
    18781 tctttaggta ttgtgaacca gcagtttatt ttgaacgtcc aaacagaaaa tgctgcaatt
    18841 gccagacgta tctattctct tttgaaagat cattatcagg tacgaagtga gctactcgtt
    18901 cgtaaaaaaa tgaaattaaa aaagaacaat gtctatatcg tccggttgaa gcaaggcaca
    18961 aaagatgtgt tgatggatct tgatatcatg gatggtatga tgttcaatgg acacgtttcg
    19021 gacgagatta tgggaaattc gcaaaaaatg cgttcttact tacgtggtgc ttttatggcc
    19081 tcaggttcag tcaataatcc ggaaacgagc cgttatcatt tggaaatatt ttctatttat
    19141 gaacagcata accaagacat ttgcgatatg ttaaattatt acggtctaca tgctcgaacg
    19201 cttgaacggc gcaatggcta tatttcttat ttaaaaggag cagaaaaaat tgctgatttc
    19261 ttaacattga ttggtgcaac caactcaatg ctccgatttg aagatgttcg aatcgtacgg
    19321 gatatgagaa attcggtcaa tcgtctagtc aattgtgaga cagcaaatct gaacaagacg
    19381 attgatgcgg cttctaaaca aatcgaaaac atcgaattga tcgaagcaaa ggtcggtcta
    19441 catgcccttc ctgaaaaact tcaggaaatc gcagaacttc ggcttcaaca tccagaggtc
    19501 agcttgaaag aattagggga aatgattccc tcaggtgcga tatcaaaatc gggaattaat
    19561 caccgaattc gcaaaataaa tgaatttgcg gaaaaactgc gtgaagaagc agtttaatcc
    19621 aaagaaaaaa ctgatacatg aagcaggaga tgcgaatgta tcagtttttt ttaaaagtta
    19681 tacttgcttt tcttccgtca aactataatt ccatctttac tggagtaaca taatttggaa
    19741 aggcttctat tgtttttaat acaatcgtat aaaaagcggg gagacaactg aatgaagatt
    19801 ttagttttgt acataaattt ttgcttaata tagaagtcct gatatagata agatcaagta
    19861 tttatctata actggcctga ttaattaaaa aactggaagg aactgctcga tcatccttga
    19921 gaactgctct tcttacttct ggtcaagatt ctcgtggttg gtggataagg atggttgata
    19981 agttcattgg attagctagt cgaaaacaac caggtgtaat taatgtagaa gttttaacaa
    20041 ggggatggag tgatttattc agtgataatc ataagttcat aaatagtaat tgagatgtat
    20101 ggttggaata cgttatacct gaacttgaac taccaagatt tggtttagaa tttgagaatc
    20161 gatgtttata aagggagggc aaaaatgcgt ttttttacaa cttttactat cattatgatt
    20221 gcagtacttt ttatttttct agatatagca aagcgaaata cagcattttt attatatcgg
    20281 gttttacttc gagcaggtct cataacattt atatcgatcg ttggattttt tctattcaca
    20341 gttattgtat ttatttggag aactccagca ccaccattgc cagagattac atatggagaa
    20401 tttcctttta gacttgagta tgaattgaat gaagaattac atgtgataga agacacgtta
    20461 atagttgaat ttgatggatt tggaatgaac gaaggcatag ggagatatcg tagatggaca
    20521 agtcgtttag ccagcggaga agatttagtg ttgctgttag aagttagtga taataagcaa
    20581 atattttatt ttccaggacc tgctaattat tacatgggag ataggttgaa tggttataat
    20641 catacttttc ctagtgcttc tttcattgaa agggaaagag gcattactag aagggatatt
    20701 ttacatgaca aagaattatt agaacaattt ggtccgcttg atcaaaatac aataaatgaa
    20761 gaagaattgt taaatcaata taatataagg ctagtgaatt gggaaatatc tgaaccgatt
    20821 gttaacaatt ttggtgatta gaaaacaatt acgataaata gaaacaagcc aaatcatcag
    20881 caagtaaaag ctgatgattt ggcctttctt atacgagtag taggaattca tagcgaatat
    20941 tattacgatc agtagatatt ttgatattaa aggagtatta cgaactagta tgtgagaatg
    21001 tagaggtgat ttgcgaataa tgagaaattt atcaaataga aacaaaatat taatcattat
    21061 tgttgttata gctgtgtttc atctgggaac gaatgcggtt ttaagtagaa taatattggg
    21121 tcctaagcca ccaagacctg cgattacccg aggggagttt gattttagat tagaatatga
    21181 agtagatggg gaaaggattg tgatagaaga tacaattgtt gcattatttg atggattttc
    21241 tgcagatgca ggtagcatgg catggtatcg cacgtggcgt ttgcatttgg cgagtgacaa
    21301 gagggcacaa caattattgt tacatgagtt agatgatggt cgaaaaatat attatcattt
    21361 tggaggagct ggattttata tggggaagtt agtagcagtg taactgtaac aaggaaagat
    21421 ttgccaggtc ttggcattcg aaactccata ttttatatcg agcgtgggag agatacatct
    21481 attgggaaca ggaaagggtt aactcttgaa gagttatata atgactttgg aatccgtctt
    21541 gttagttggg aacacaatcc accaatagaa aatcgctttg aataatcgaa atctattaat
    21601 ccatttcatc agccagtgaa tgttgtaatg gaacaatttg gtttagtgtt caaaaccatt
    21661 gaaatactag gctgtgatca gcaaaaatgt atctgaataa aaaagagagc aactattaaa
    21721 tgagaggagt taggagttgt tagctttata cgaatcaaag tggcaaatac agatgcgtat
    21781 gcatttgtgt ttgccatctt gcgtttggta gacgagatag gtataagcta aagaaacaac
    21841 ttgtttaaaa taaacgacct agctcaacaa acgaccatct aatcaactaa cagcatacat
    21901 aaaagcaact atttttgaag tagaattgaa atcgggataa cttaaaaagt gattgggata
    21961 ctagtgtgaa tcaccgtaca taaatattac tcgttaaggg aatttgagaa attttgtatg
    22021 aaaatttaag aattagttag tatgcgcaag tcaaaaatat gaataaatta tttggtggag
    22081 ataatttgct cagcttgtca taagaaatga gataatctgg cagatgcaag cttttggaca
    22141 ccatttattc ggaataagga ggctgggaca gaagcgttta actccaagaa ataagaagga
    22201 gttgcttctg cttccgccgt ttataggtgt ttagagcgtg agacaaaagt gttctttact
    22261 tttgtcctac gctccttatt tttttatttt gtttggatca attcttttaa ttcctctatt
    22321 gaaatagatt catcaaagat ttctttaccc aagatgacag tcggaacaaa tcgaatatgt
    22381 gcttgctcag cttcgttgat gacagcttgg ctcagctgtt cgtctttttg ttctgtcagc
    22441 tttagtttat ctactgcaaa atccgctact tcttggagag aaagatgttt ccactcgtct
    22501 tgagtatcga atatttgttt gatctctttg atcgcttttt gaccatcgct gattgtgaga
    22561 taccggtgca tcacgtttcc tcgctgcaag ctttcttttt ctttatcgaa caatttgatc
    22621 actcgctgca attgcccgct ttggactgct tcttctaata gctcataaga ttcttcaaac
    22681 cattgtcggc aatacggaca agataggttg atgaattcga ccattttttt aggagcattc
    22741 gatgaaccat aaaggattcc tttttgtgta tttgttttcg ttgcatcaat cactgatata
    22801 tccatgttaa aacacctctt tcgcttatag tttatacatg tctgacaaaa aaaggcaacg
    22861 atttagactg tttgtctaaa aattagatga ccaattcaaa tagacatatg tgaaatattt
    22921 ttataaataa aagaaaaaat ggaatgtttt acgttataat ggaacagata ataagagaga
    22981 atatgaacaa ggatgatgaa tcatgcgaaa aatgaaaaca atggatggca atacggcagc
    23041 cgcatatatt tcttatgcat ttaccgagat tgctgcaata tatccaataa cacccagttc
    23101 gaccatggct gaattagtag atcaatggtc agcagcagga aaagaaaatg tattcggtca
    23161 accagtaaaa gtagtagaaa tgcaatccga agcaggagca gctggtgtgg tccatggatc
    23221 attgaaaact ggtgcactga caactacata taccgcttct caagggctgc ttttgatgat
    23281 accgaatatg tacaagattg ctggggaact actgccaagt gttttccatg ttgctagtcg
    23341 ggcagtgacg acaaatgcgt tgaatatttt cggagatcac ggagacgtga tggctgcaag
    23401 acaaacagga tttgctatgt tagctgaaag cagtgtacaa gaagtgatgg atttagcacc
    23461 tgttgcccat cttgcttcta ttgaaggaga agtacctttt ttgaatttct ttgatggatt
    23521 ccgtacgagt cacgagattc aaaaaatcga agttttggaa tatgaagaat tagctcctct
    23581 gatcaatcag gagaaattag cttcatttcg taaacgaggg atgaatccca atcatccgac
    23641 tgttagtggt acgaaccaga atccagacat ccattttcaa caacgggaaa cgatcaaccc
    23701 ctattatgat aaattacctg aaatcgttca aaaatatatg aaagagatca accgattacg
    23761 aggaacttct tatgacttgg tgacttatta cggagcagac gatgcggaag aagtcattgt
    23821 ttctatggga tcagttgcac agacgattga acaaacgatt gattacttga atgcaaacgg
    23881 acggaaaaca ggttttttga atatccatct ttatcgtcct tttccagtgg aaacattcgt
    23941 gcaaaagttg cctcatacag taaaaacaat tgctgttctt gatcgaagta aagaaccagg
    24001 ggcaggtgga gaacctctct tattagacgt tcaaagtgcg ttatatgatt ctgaacttcg
    24061 cccggctgtt attggtggtc gctacggatt ggggtctaaa gatgtgactc ccgaccaaat
    24121 ctcagcagtt tttgatgaat tgaaaaaaga cccaagcata cgcaaaaaac ggtttaccat
    24181 agggatcgtg gatgatgtga cttatcaatc tttggaacca aaggaaagtc tggatttgac
    24241 agaaccgcaa acattccagg ccaagttttg gggatttgga tctgacggaa cagttggagc
    24301 aaataaatca gctatcaaga ttattggaga tcacaccgat aaatatgcgc aaggctattt
    24361 ttattatgat tcgaaaaaat ctggcggtct gactgtctct catcttcgtt ttggagatac
    24421 gccgatacgt tcagcgtatc ttgtcgagca tgcggacctt gttgcctgtc acacaccagc
    24481 ttacctgcat agctatgact tggtcaaagg attgaagcca ggtggcattt ttttattgaa
    24541 tactctttgg agcgacgaac aattagaaac gcatctgccc ttaaaattaa aacgttactt
    24601 ggctgaaaat aacattagat tctatacgat caatgccatg agattggccc aagaagtcgg
    24661 tttgggacgc cggatcaata cggcaatgga aacggctttt tttaaattag ctgacatcat
    24721 tccttttgat gaggtgcttc cgctgctaaa agaagaagcc ctgaaaagct atggacataa
    24781 atcaatggct atcgtagaaa aaaacataca agcaatcgat cggacgaccg aactgcttca
    24841 tcaagtcgcg atcccagacc attggagaac gttggagatg cctgaaaaaa tcaataaaag
    24901 gaacactggt tatatacaag aaatacttga accagtgaat gcacaggaag gaaatcaact
    24961 ctctgttggt acacttgtag caaatggaat gacaactggc gaaatgcctt taggaacggc
    25021 ttctatggaa aagcggggcg tcgctttaga agtacctgag tggattagtg atcgctgtac
    25081 gatgtgtaat gaatgtgcct ttgtttgtcc tcatgctgcc atccgtccat ttttagcaga
    25141 tgaagaggaa atggaagaag cgccagaagg atttatcgtg agagaaatgc gaggcgcaga
    25201 cggcgtaaaa taccgtatcc aagtttcagt ggaagactgt acaggttgtg gtctgtgtgt
    25261 agaggcttgt cccgcaaaag aaaaagcttt agtgatgaag ccttatgaag aacagaaaga
    25321 acaagctgtg aattgggctt ttgcaatgac attaagacaa aaagcaaatc cagtcaaagg
    25381 aaagaatact gtcttgtcta cacaattcaa tcaaccgctt cttgaattct cgggcgcttg
    25441 ctcaggctgt ggcgaaacac cttatgtaaa attattgaca caaatgttcg gtgatcgaat
    25501 gatgatcgcg aatgcgacag gctgttcttc tatttggggc ggtgcttctc ctgcttctcc
    25561 ttatacgacc aatgaatgtg gtcaagggcc tgcatggtca aattctttat tggaagataa
    25621 tgctgaattt ggttatggta tgcttctagc ggcacagact cgtcgcgagg ctttggcaag
    25681 ttcaatcaaa gaagtattgc caaaagtatc agaagaacta cgagaattga tggaagactg
    25741 gttggtacac atgtttgaga gcgaaggaac gcagcaaaga gcagcaaaac tgcaagctgc
    25801 gatcgaaagc gaacgatcaa ttgtccctga actggaggat atatacaaga gaaaggatct
    25861 attcgtaaaa aacagccaat ggatgattgg tggagacgga tgggcttacg acattggttt
    25921 tggcgggatc gatcatgtac ttgccagcgg agcagacgtg aatattcttg ttttggataa
    25981 cgaagtctat tccaatactg gcggtcaaac ctcaaaagca acaccagctt cggcaatcgc
    26041 taaattttcg gctagtggaa agtatgcttc aaagaaagat ctaggaatga tggcgatgac
    26101 atatggaaat gtttatgtgg cgcaaattgc ttcaggtgcc aatcaaatgc agacgataaa
    26161 ggcttttgaa gaagcagaaa agtatcctgg accttcttta atcattgcct atacgccttg
    26221 tatcacacat ggtttgcttg gtggaatgaa agaatcgctt aaagaagcaa aagaagctgt
    26281 ttcttctggc tattggtcat tatatcgata taaccctgca atggtcgaaa aaaacaagaa
    26341 cccaatgact ttggactaca aaaaacctga tttctctgct atgcctgatt tcatgaggaa
    26401 acaagttcgc ttttcctcgc tagagaacgc aaatccaaat cttgcaaaag ctttatttga
    26461 aaagactatc caagacgcta aaacacgttt ttacaactat gctgcactga cgggagaatt
    26521 agaaaaaatc aagaaacgac ttgaaccaga ggaatctatc gatgtacccg ttaagactag
    26581 aaaagaaaaa acgacggacc ttgaagcaga agaaagaaga gctgccagag cagaacgacg
    26641 taaaaaaatg gaacaagcta caaaagaaaa aaaggaaaaa taacagaaaa aatataatga
    26701 ataattctct gaagtttaaa aatatttatt gcagagaaaa ataaaacgca tacttgcaat
    26761 tttcctgaaa attatgcgtt tttctcaaga aaaatacgag cagatcagcc tctaaaatta
    26821 aaacatatga tatactagag aggaaatctc aaaagagaag agaggctttt tatgtcattt
    26881 caaatcgggg aactttttaa tttggactat tggcagcaaa tgttttccgg tgacttgttc
    26941 tctctgaatt ttttggtgaa catacttgat attctggttg tctggtatct tgtatacaaa
    27001 ctgatccaac tgctgcgagg cacaaaagcg attcagcttc tgaaaggtgt agctattttt
    27061 attgttatcc gaatcgtagc ggaaatcatt ggtctgcaca ccttgtcttg gctgatgaac
    27121 caagtcatta cgtacggggt gatagctgct atcgtgattt ttcagccgga aatccggaga
    27181 ggactggaac atcttggacg cagttcgctt ttcaaacaat caaaaagcga acagcaggaa
    27241 gatgagaaga tgatcttgtc ttttgacaaa gccatccaat atatgtccaa gcgaaaaatc
    27301 ggtgcgctaa ttactatcga acggcatact ggattagatg agtatattga gacaggaatt
    27361 gcattagatg cagatatcac tggagagctg ctgatcaata tctttattcc taatacaccg
    27421 ctgcatgacg gtgcagttat tgtaaaagaa ggaaagatcg cagtagcaag cgcctatctt
    27481 cctctatcag aaagtatgtt gattccaaaa gaattcggga ctcgacaccg ggcagctgtc
    27541 ggtataagcg aagtcagcga cgcgattacc atcgttgtct ctgaagaaac tggggatgtc
    27601 agcattacat tagataatga actgatggct ggcttatccc agcaagaata tttggcaatt
    27661 ttgcgtagag aactgattgc tgaagagaaa acaaaaaaca agaaaaacgt gattcaatct
    27721 ttcctagacg gtatgactaa gggaggggat cgcaaatgat ctcaaaagag aaacggacaa
    27781 gcattcttta cagttttatt gcattgctgt tcagtattgc gctgtacttc aatgcaaatg
    27841 gccagtccgt tccaaatacg ctttctggaa atgaagcgta taatcaaaca gttactgggg
    27901 tgccaatcaa gatttcttat gattcaaagc gctactatat ccatggatat gaaaatgcag
    27961 tgactgtcaa actttccagt gcaaaccgag ttcagttgaa tgcagaggca aacgaagata
    28021 cacggatgtt ccgcgtcaca gcagatatca ctaagctggg cgaaggaaca catgaggttc
    28081 cgctaaaaat tcaaaatttg agcagtgctg tcacagcaaa gatcgaacca tcgactatta
    28141 cagtaacggt tgagaaaaag gtaataaaag atttcgatgt ggaaacgttg attccttcaa
    28201 cgttcacacc atctggatat gaacttgatg atacttccgt tagtcctaaa aaagtctcta
    28261 tcactacagg tgacaaaaca ttggcagaaa tcaaacgagt agtagctgat gtggacgctt
    28321 cgatggtcac agatgatggg attaatagtg aggtcccaat acaggcgctg aatgcagcag
    28381 gggaagtatt aagtatcgtc tctgatccag tgcaagtaac agtgagagct gattcgatca
    28441 agccaagcaa gtcggtacga ctttatggga tacagcaagg aacaccagct gccggggtag
    28501 aaagctatga tttcagtttt tcaaaactgg aagcagaagt aagcggctct agcgatttgc
    28561 ttgcatcgat cggtgacagt attgctgtac cgatcaatgt ctcaggtatc aatcatcgaa
    28621 caactcgtaa aattgaaata ccagtagaag aggggttaag tatcaagccc gaatcagtta
    28681 gtgtggagat tacacccgtg ttgcaaacgt caagcagtag ctctgcttcg acgagcagtc
    28741 ggacacaaga aaatacaagt acgagtagaa gtatttcatc tgctgattca ggaacggaac
    28801 catcgacaac aaccatttct tccagttcgg aatctgggac aagcagctcg accacaagta
    28861 cagaagaaac atcagaaaca agcgaatcaa cacaaatgag cagaacaaat tcctcagaaa
    28921 attaagttaa aggagtaatt ttacaactat gggtaaatat tttggaacag acggcgtacg
    28981 cggtgaagcc aacaaggaac ttacgccaga acttgcattt aagctgggac ggtgcggagg
    29041 ttatgtccta agccagcatg aagaagaagg aaaacgccca cgcgtgttag tagggcggga
    29101 tacaagaatc tcaggtcaaa tgttagaaga agcgctgatt tcaggacttt tatcggtggg
    29161 tatcgaagtg ttccagctag gtgtgatctc tacaccaggt gttgcttatc tgacacgttt
    29221 gcaaaaagca agcgcagggg tcatgatttc tgcttctcat aatccagcac aagataatgg
    29281 aatcaaattt ttcggtgcag atggttttaa actagtagat gaccaagagc tagaaattga
    29341 agcattatta gatgctgaag tggatgaatt gcctcgtcca tcagctgaag gattgggaac
    29401 tgtagaagaa tttccagaag gcttattgaa atattctcaa ttccttgtac aaacgatcaa
    29461 cggtgacctt tccggtttga ccatttgtgt cgatgcagca aacggagcaa cttcaacaag
    29521 tgttaatcgt ttgtttgccg atcttgaaac agatttttat accatgggca caaatccaga
    29581 cgggttaaat atcaatgatg gcgtaggctc tacccatcca gaacgtttag ctgagatggt
    29641 cgttgaaaaa ggagcagatg ctggactagc tttcgatggc gatggcgatc gtattattgc
    29701 ggtggatgaa cttggaaaca ttgtcgacgg tgataaaatc atgtttatct gtgctaaata
    29761 cttggcagaa aaaaatcgat tgaaaaaaga tacgatcgtt acgactgtca tgagtaactt
    29821 aggattccac aaagcggtgg aaggaatcgg tttgaaagat gtcgttacac aagttggtga
    29881 tcgttacgtt gtagaagaaa tgcgcaaaaa cgattataac tttggcggcg agcagtctgg
    29941 gcacatgatt ttcttagatt ttaacacaac tggagatggg atgctttcag gaattcagtt
    30001 actgaatatc atgaaacaga caggtaagaa attatcagag cttgcagcag aggtaacaat
    30061 ctatccgcaa aaattagtga atatccgagt aagtgataaa aacggtgcga tggatgttcc
    30121 ggcaatcaaa aaagtgattg aagaggcaga agctgaaatg ggatcagaag gaagaatcct
    30181 agttcgtcct tccggtacag aaccattact tcgagtgatg gcagaagcgc ctactgatga
    30241 gaaagttaat tactatgtcg ataaaatcgc ggcagtagtc aaagaagaaa tcggactgga
    30301 ttaagagata ttttcataaa aaataaaaga acaaggacca gtgtcagatg tctgacactg
    30361 gtccttgttc ttttattatt ctactgtcac tgatttcgct aaattacgtg gtttgtcaac
    30421 gtcgaatccc cgtaatagcg ttgcgtaata tgcaagtaat tgagtcggta cgactgtggt
    30481 tagagtagtt agataagggt ggacagttgg tagcaccagc tggtcacttt ccttttctaa
    30541 tccctctgag acaataacta gtgttttagc gccacggctt tcgacttctt tcaggtttcc
    30601 tcgagtatgc gcaccagtca cttcttcact gatgattcca atcactggtg ttccttcttc
    30661 gatcaaagca atcgttccat gtttcaattc gccagcggca aaaccttctg cctggatata
    30721 actgatttct ttcaatttca aagcagcttc aagagaaacg ttgtagtcag cgccacgtcc
    30781 aatgtaaaag gcatttcttg tatcgctgag atatacttct gccaactctt ctaaataatc
    30841 tttttgggcg atgaccgatt ccatcgcatt agcaactaaa ctcaattcat gagtgatatc
    30901 gaatacaaga gaagtttcaa attctttttt atcaccgata gctttggcta ataaagttag
    30961 tacagctatt tgtgcagtat aagctttcgt tgatgctact gcgatttcgg gtccagcatg
    31021 caatagcagt gtaaaggatg cttcccgcga aagagtagaa ccagcaacat ttgtaatcgt
    31081 aagtgaaggg tagccgagac ggttgatttt taccagaact tggcggctat ctgctgtttc
    31141 gccactttga ctcaagaaaa taaagaatgg tttttttgaa agaagaggtg tgttatagcc
    31201 aaattcactg gctaaatgaa cctcaacagg gatctgtgtc aatttttcca gagtctgttt
    31261 acctgctaac cccgcatggt aactagttcc gcaagcaacg atatagattc gatcgctatc
    31321 agacaatgta tcgaccagct tttgatcaag agagacacga ttgtcttctg ttacgtattt
    31381 ttgagcgata cgacgcatga taataggctg ttcgtcgatt tctttcagca tataaaaagg
    31441 ataggtacct ttctcgatat ctgaagaatc tacttgtgct tcgaatgaat aacgttctac
    31501 gatgtcacct gcaaaatttt gaataataac tttttctgat gtgacggtaa ccatctcttc
    31561 atcttcaatc tcaacgaatt ccttcgtatg agcaagcata gccattgcat cgctggcaat
    31621 cacattgaat ccatcgccga gtccgattaa tagtggactt ttatttttag cgacgtaaat
    31681 aacatcaggt gctgtccgat caatcaaagc gaaagcgtaa gaacctttga tgacccgcaa
    31741 agcttttaaa aacgcctctt gcgttgtagt atccttcgcg aaagtttcaa ttagatgagc
    31801 gataatctca gtatcggtct caccaataaa gtggtcattt gctaaataag caattttaag
    31861 ttcttcaaag ttttcgatta cgccattgtg aacgagcacg aagcgtccac tttgggaagt
    31921 atgtggatgg gcgttttctt cagaaggttg cccgtgagtt gcccagcgtg tatgaccaat
    31981 acctatattt cctgtagtat caatatttat tttgtttttc aaattttgaa ttctgccttg
    32041 tgctttgacc agataatcag ttgtgccatc tgagacaaag ataccagctg aatcataacc
    32101 acggtattct aatttttcta gaccgtcaat caatcctggt gttacatttt ttaatccaat
    32161 cattccaaca attccgcaca taaaaaacat cctactttct ataatgaatt cgtatgaatg
    32221 cttccagctg caggaaaatc cttttgtttt atctttgcag atattttttg tggattttct
    32281 gtagagtgca gtccctaggg ccatccgccg aatctttcga taaaccctct cctcgtcatc
    32341 tgtttacaca gacctggcgc tatcctaaaa ggtataacta tcctcctctt ttgtttttta
    32401 tctggaaaca tttaaaatca taaccttttg cgaatgttct tgtcaacaaa aaaagaataa
    32461 aaaataaatt ggtatatatc aaatgctttg tcaaatgctc tttttatgtc aaaaaataat
    32521 gggtttttat ctatcatctt tttcaggagt tcggtacaat agaaagtaat cggatagagg
    32581 aggagtaaaa atggattcaa aaattgagat catgacactg ggaatgttaa aaaaacaatt
    32641 gtccaaattc gaagcatcag ctggtgtatc agatgatacg aaaatatttt tagatactgg
    32701 ctgggatagc atccaagaaa tcgcacctga tgccttggaa gttgctgaag ctcgtgagtt
    32761 tacggtagaa gatgaattga caaaagagtc gttttccggg tatgcgcggg aagaaaaagc
    32821 agagcggttc gacacttctg agcagtcaga aactgtgatc gtcataaaaa acttgtatta
    32881 gaaaggagaa agaatgaata agagacgttg ggcagcagtt ctgattgctg caggattatt
    32941 tgtggtttcg ttagtttcag ctagtttgac taagcctcag gaaaacgatc aaattgaggg
    33001 attgaacagt tggttatatg gggatgaaga actagcttca aatgtgttgg aagagggaga
    33061 cagtacgcaa aaaatcgtaa aattatcagt agatggaacg attgctgcgg gagggtcttc
    33121 aggtttattt gcttctgaag gttacaatca taaaaatttt atggaacaac tgaaggcgat
    33181 tgaagaagat ccaacagtca aaggtatctt tttagaagta aattctcctg gtggcggtgt
    33241 ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt cgtaaagaac atgatatccc
    33301 aatgtatgtc agcatgaaaa acatggcagc tagcggtggc tactatattt ctgctcaagc
    33361 agataaaatc tttgcaacag aagaaacagt gaccggctca attggtgtga tcatgtcagg
    33421 attgaattat tcaggacttc tagaaaaact gggtgtggaa gatacaacag ttaaaagcgg
    33481 agcgttgaaa gatatgggtt caggaacgcg accagagaca aaagaagaag aagcggtgct
    33541 tcaagcatat atcgacagcg catatagtcg ttttgtagca atcgtcagcg agggacggaa
    33601 taaatctgaa gaagaagtca aaaaaattgc tgatggaagg atttatgatg gtgtccaagc
    33661 aaaagaagta ggtttagtcg atgaattggg atttcctgaa gatgctttaa aagctatgcg
    33721 aaaggaacaa cagcttgaag atgcggaact agtagaatat tcttccacgc catctgggtt
    33781 tgctagtaca tggttaggaa gtaaactagc agaattccag ggattgaagc cttctgagac
    33841 tagtcagatc ttatccgttc ttgagaatct agggacagcg gaatcaccaa aagcaatgta
    33901 ttattacgga ggtgagtaag catgtcagag ctttcaaatg aaaataagga acaagaaaaa
    33961 gataagcaac agcagcctgc tgtaggacct gactccaact tttcccaaaa ggtactgagc
    34021 tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa agaaacaacg taaatggaaa
    34081 aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg attttcctaa gtatttctat
    34141 gcaggttttt ttatccgaat gtttgctttc ttggtcgatc ttttgtgtat cggagctatt
    34201 acgcgtttaa cacttggtat tgccaaaaat ctaggttggc ttaatttttc agattcttat
    34261 ctgagtatct acggattcac agccttactt atttatctag cgtattttat tttattgacg
    34321 aaattcaatc atggtcaaac gattgggaaa atgatctttg gtattcgagt catcagcttt
    34381 gatgaagaag aacttagctg gtcaaccgtt ttagttagag aaggtgcttg ccggttcatt
    34441 ctaaaatttc ctttgctgat gttagggtat cttccagcaa tctttaatca gaaaaagcaa
    34501 catctaggcg attttttcac taatacaagt gttgttacga tcaatctgat caaagcattc
    34561 aaccaggaag tcaacagctg atgagcaata aaaagcagta acagttgaaa gcaccttcta
    34621 aaagtcagat ctaaaatcta gattttagag ggtgcttcag ttatttttaa ttgtcatgct
    34681 ttttttgttt tgctactatt gaacatgagc tgaacatgag caagaggagg acactgcgtg
    34741 aagccgattg attttccaga aaaatatgag aatttaatgc gagtagcaca acaagcacta
    34801 gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac gagcttacga gttgaaagaa
    34861 agctttgagg ctaatagcct gcttgttttt tgtctttacg agttggacga gaaaaaagaa
    34921 gcattgaagc aagcactctt gcatgaaaaa cagtatcttc aaaatgagga gtttgctgaa
    34981 ttttattttg acttgttaat cagtacacaa gattttttat atgctagaaa attgatagca
    35041 agtacagatt tttatgaagc tttcgagcag cgggtcattg aaaaaatcca atttgcagaa
    35101 gaattatccg gacaaatgga gaggcagaag atcaaggcac tccacaataa atcggaggaa
    35161 cttccttcgt tggaacctgc acgtcagctt gcgtctattg atgagatcga acagcttcct
    35221 tatcatgagt ttatccaaac agcgagtaaa cttattgtat cgccagaggt ccatatttta
    35281 gcaagggcaa aattactgga gacacttagg caattgaacg aatgccatcc tgttttctat
    35341 ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag atctgccaaa gccgcagcag
    35401 caggcaagtt accagcaatt atgtgtattt tcagatcgtt acggtaatga agatgcgctg
    35461 cttagttccg tgttaaatga agaattcacg ttgcaatctg caattgttta tcctgtctat
    35521 gatacataca tcgaagaccc gaagcgctgg tttcaactga ctgtagaggc gtgtacagga
    35581 aaaacgatgt atgaaggtac agaagatgaa aaacaagatt ttttaaaaaa aagagaaaaa
    35641 attttgcagc aaatgatctt cttccattag attttatcag tgaaaaagat aaaaatgtta
    35701 tgaaaaacaa ggattctatc cattcaatat gtttacaatt tgaaagggtt agtgtactat
    35761 ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa tttctaaact cggagggaat
    35821 taaatgactg caacatttga aaaaaaaggc acgaatgatg gtgttttaaa atttacgatt
    35881 gcgcaagacg aaatccaaaa aggattaaca actgcgttta ataaagtgaa aaaaagcttg
    35941 aacgtacctg gattccgtaa agggaaagtt tctcgtcaag tattcaaccg tatgtacggt
    36001 gaagaagctt tatatgaaga tgcattgaat gctgtattac cagaagctta tgaagctgct
    36061 gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa tcgatgttga aagcatggaa
    36121 aaaggtcaag actgggtaat cactgctgaa gtaacagtga aaccagaagt aaaattagga
    36181 caatataaag atctagttgt tgaaaaacaa gaccgtgaag taacagatgc tgacgtggat
    36241 gcacgtatca aacgtgaaca agaagcacaa gctgaattag ttatcaaaga agatgctgca
    36301 gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct ttgtagatgg cgaagcgttt
    36361 gatggcggaa aaggcgaaaa ttactcactt gaattaggtt caggctcatt cattccaggt
    36421 tttgaagatc aattagttgg tcataaatca ggtgaaagtc tagatgtaaa tgtaactttc
    36481 ccagaagatt atcaagcaga agatcttgct ggaaaagaag cagtcttcaa agtgacaatc
    36541 catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg aattcgcaaa agatgttgat
    36601 gacgaagtat caacattaga cgaattgaaa gaaaaatatc gcaaagaatt agtagaaaca
    36661 aaagaaaaag cagcggacga tgcaaaagat gaagcagcga tccgtatggc tgttgaaaac
    36721 gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg aagtacatcg ttcaatggac
    36781 gaattcttga acaacatgca acgtcaaggt atctctcctg aaatgtacta tcaattaact
    36841 ggttctacag aagaagactt gcataaacaa ttcgaaggtg aagcagaaat gcgcacaaga
    36901 acaaatcttg tgatcgaagc aattgcagct gctgaaaatc ttcaagcaac agaagaagaa
    36961 atcaatgcag aaatccaaga attatcagaa acatacaaca tgccaatcga acaaatcaaa
    37021 cgcgtgttga cagaagatat gctggaacat gatgtgacaa tgaaaaaagc aatcgaattg
    37081 atcacaggta cagcgacaga agcgaaagaa tagaaatcaa gtaataaatc atttctgaag
    37141 aagctacttc tagaaaattg tttattcgaa ataagggact gtgacacgat tttgtcacag
    37201 ttccttattt tttgttaaga gtaaaaaatg caaaggtata ggtcagttga tcctttgtta
    37261 aacacttttt ctttttacta aactatgtta tgatagtaaa agactgtaaa gtctttgata
    37321 gtttcttgca tactttttgc aagttaaggg gtgaagacca tgtatgacaa tccaagtagc
    37381 aatgagaccg tacgctgttc tttttgcggg aaaacccaag aagaagtgaa aaagatcgta
    37441 gctggtccag gggtctacat ctgtaatgaa tgtatcgacc tatgtaaaga aatcattgat
    37501 gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag tccctaaacc actagaaatt
    37561 cttgaagtat tgaataacta cgtgattgga caagatcgcg caaaacgctc gttagctgtt
    37621 gccgtataca atcactataa acgtgtcaat cagcaagcac aagaaaacac agatgatgtt
    37681 gaactgcaaa aaagcaatat ctgcttgatc ggacctacag ggtctggtaa aacattttta
    37741 gcacaaacat tagcaaaaac attgaatgta ccttttgcaa tcgcagatgc gacaagtttg
    37801 acagaagctg gttatgtggg ggaagatgta gaaaacattt tgcttaaatt gcttcaatct
    37861 gctgattaca atgtggaacg tgcagaaaaa gggatcatct atattgatga gatcgataaa
    37921 attgcgcgta aaagcgaaaa tgtatctatt actcgtgacg tttccggcga aggtgtccaa
    37981 caagccttgc tgaaaattct agaaggaact gttgcaagcg tgccacctca gggaggacgt
    38041 aaacatccac atcaagaatt tattcagatt gatacgacga acatattgtt catcgtaggt
    38101 ggtgcatttg atggcatcga aacgattgtg aagaatcgta tgggcgaaaa gacgattgga
    38161 tttggtacac aaaaccaaaa actttctgaa gacgaaagtg tcatgcagca tatcattcct
    38221 gaagatttac taaaattcgg attgatccct gagtttatcg gacgattacc tgttatggca
    38281 gctttagaga aattgacaac agatgatctg gtacggatct tgactgagcc gaaaaatgcg
    38341 ctagtcaaac aatatcaaaa acttctttct ctagataata ccgaacttga attcgaacct
    38401 gaagcgttga gagccattgc gaaaaaagca atcgaacgca ataccggtgc tcgaggactt
    38461 cgttccatca ttgaagaaat catgatggat gtgatgtttg atatcccttc aaatgaaaac
    38521 atcgaaaaag tgattatcac aaaagaagca gctgaattgt ctggtgagcc aacagtaatc
    38581 tataaagatt cagagaaaaa agcgggttga tgaaaagagg ctgccgtaaa agtcattttg
    38641 gcttttgcga cagccgcttg ctttattcat caccttgtta aggagagaga ccatgaaagt
    38701 ccatcaagct gaaattatca taagtgcagt ggcaccaaaa caatatccag atacacaatt
    38761 gccagaaata gctctggcag gacgatcaaa tgtagggaaa tcttctttta tcaacacatt
    38821 gattgatcga aagaatttag cccgtacttc cggtaagcca ggaaagaccc agacattgaa
    38881 tttttatttg atcgagaacg cattacattt tgtcgatgtt ccgggatatg ggtatgcaaa
    38941 agtatccaaa actgagcgag caaaatgggg aaagatgatc gagacatatt taacgactag
    39001 agaccagcta cgtgcggtcg tttctttagt agatttgcgt cataagcctt ctcaagaaga
    39061 tatccagatg tatcaatttt tgaagtatta tgatatccct gtaattgttg ttgccactaa
    39121 ggcagataag atccctcgtg gtaaatggaa caagcatgag agcgaaatca aaaaagcgtt
    39181 ggattttgat cgcaatgaca cgtttattct cttttcttcc gttacaaaac agggaaaaga
    39241 agaagcgtgg gcagcaatcg aacaagcaat tgaatcataa aaagaataaa aaagacttac
    39301 cagtcaaact ctttcataaa agtttggctg gtaagtcttt ttttgagctt aacttacttt
    39361 tgtttcttgt cttttttctt ttcattttgt ggctgtggtt ttagaaatcg atcgatctct
    39421 tcttttggtg caactgtttt ttccggatcg acagcaaatg attcgaaaag tttatctaaa
    39481 ttatctgtac gttcaaattt caaccccatt gtaaacacct ctttgtttct ttttacttag
    39541 tctatcatgt aatcgtccaa attggtttct aagacataaa gttttagttt ttctttatcg
    39601 ttggttataa ttaaattata aaaagatttt aaagaggtga gaaaatgatt ggcgaagata
    39661 aattattgat gactatccag ctcacagact taaatgaaga agaaaaaaaa caggaaataa
    39721 gcgattgggc tattttgaca cgtctgttga tccagccaac atttatccgc tctttcacac
    39781 aacaagcgga tccgtatgat cttcgaaagc ttcaagaaaa aataatgcta gcatcggtaa
    39841 gagacgaaag ttggttagtt gtaggaaatg acgagaacga atgtagtttt cgcttggaag
    39901 ataatcaatt attgataaaa aatgtattgt ctatctctgt tttcaaagaa aagcaatttt
    39961 tgattcgaga cttcattcag aaaaaaatga ttgctcatgg ggtgttcgcc tatatgcgag
    40021 cttatagcga gtttatctat cacaatacaa aacagatctc tcaacggcta ttgtttgaga
    40081 aaaaggatga aatcgaacat ttacctaaaa tgaaacaaca aaacggggaa gtagtagtcg
    40141 attgcaacca atttccagga tacgatcttt tttaccaagg tttatgtttc acttcgtgtt
    40201 gggaaatgta ttatagtcgc tattatcatc aaatcatccc taaaccgatt tttctcgaca
    40261 tccaacaagt agaaaaagtg aaggaattag aaaacgaagt catatgtatc cagctttatc
    40321 gagacccctt taactggcaa aagccaaaca atcaaatgtt tcaatcctat tttagggacc
    40381 agcttgggtt tgatcatctt gcttgggaca atggtgtcgg tttattgaaa ccgccattcg
    40441 ttgaatacat ttacactgac catacgatcc aatctgtcca gtatcagaat gcacaaatgc
    40501 aacctgttcc taaaaagaat gcttcatttt ttgtaacaaa aagttatgat attcaacaag
    40561 gagattataa agaaaggcgt gttcgcggaa cactaaatgc tcaggcgtat tttccttggg
    40621 ttgacgagaa tcgctcgcgc atgatgtgct ataaagttat cgatccgact gttgctttgg
    40681 ataatggaat cgaagcttat tgttattata ttagggaata tctagaagta gaggtaacag
    40741 atgaaaaata tcaagagtat cttttatcct taagaatcta tgttccttca gaatccctgt
    40801 cagaattacc gttaaaggaa atcaaacata gactctctga tgttaccttc aaacgaataa
    40861 agaaaaagcg gcgaagtatc caattcgatg tcaagaaagg tgagcagcat ctgcgtgtgc
    40921 aatttttaga ttaccgagaa ttagaacaat tgattacttt acaaaaaata tgaggtgaag
    40981 aaaatggata aacaactcta ttatcgaata atggttccgg catcattatt atccgtctgt
    41041 ctcttttttg ttcctaattt tcctattcga tcagtcatcg gttttttggt catgtttggc
    41101 gggatgggac tttattatta tcttcttca
//
LOCUS       DAZUKD010000009        40726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000009 DAZUKD010000000
VERSION     DAZUKD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.009"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1240
                     /locus_tag="ACG8N9_000397"
                     /pseudo
     CDS             72..1240
                     /locus_tag="ACG8N9_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1366..2445)
                     /locus_tag="ACG8N9_000398"
     CDS             complement(1366..2445)
                     /locus_tag="ACG8N9_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4896029.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2548..2988)
                     /locus_tag="ACG8N9_000399"
     CDS             complement(2548..2988)
                     /locus_tag="ACG8N9_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4896030.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3045..3455)
                     /locus_tag="ACG8N9_000400"
     CDS             complement(3045..3455)
                     /locus_tag="ACG8N9_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4896031.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3635..4069)
                     /locus_tag="ACG8N9_000401"
     CDS             complement(3635..4069)
                     /locus_tag="ACG8N9_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4896032.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4169..5017)
                     /locus_tag="ACG8N9_000402"
     CDS             complement(4169..5017)
                     /locus_tag="ACG8N9_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4896033.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5406..7172
                     /locus_tag="ACG8N9_000403"
     CDS             5406..7172
                     /locus_tag="ACG8N9_000403"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4896034.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7274..8533
                     /locus_tag="ACG8N9_000404"
     CDS             7274..8533
                     /locus_tag="ACG8N9_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4896035.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8680..9981
                     /locus_tag="ACG8N9_000405"
     CDS             8680..9981
                     /locus_tag="ACG8N9_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896036.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9984..10835
                     /locus_tag="ACG8N9_000406"
     CDS             9984..10835
                     /locus_tag="ACG8N9_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4896037.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10848..11663
                     /locus_tag="ACG8N9_000407"
     CDS             10848..11663
                     /locus_tag="ACG8N9_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4896038.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11814..12980
                     /locus_tag="ACG8N9_000408"
     CDS             11814..12980
                     /locus_tag="ACG8N9_000408"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4896039.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13210..13857
                     /locus_tag="ACG8N9_000409"
     CDS             13210..13857
                     /locus_tag="ACG8N9_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4896040.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13941..14885
                     /locus_tag="ACG8N9_000410"
     CDS             13941..14885
                     /locus_tag="ACG8N9_000410"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4896041.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15020..16471
                     /locus_tag="ACG8N9_000411"
     CDS             15020..16471
                     /locus_tag="ACG8N9_000411"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4896042.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16492..17328
                     /locus_tag="ACG8N9_000412"
     CDS             16492..17328
                     /locus_tag="ACG8N9_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4896043.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17328..17987
                     /locus_tag="ACG8N9_000413"
     CDS             17328..17987
                     /locus_tag="ACG8N9_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4896044.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18113..18802
                     /locus_tag="ACG8N9_000414"
     CDS             18113..18802
                     /locus_tag="ACG8N9_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896045.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18808..19764
                     /locus_tag="ACG8N9_000415"
     CDS             18808..19764
                     /locus_tag="ACG8N9_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4896046.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19808..20320
                     /locus_tag="ACG8N9_000416"
     CDS             19808..20320
                     /locus_tag="ACG8N9_000416"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896047.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20576..21124)
                     /locus_tag="ACG8N9_000417"
     CDS             complement(20576..21124)
                     /locus_tag="ACG8N9_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4896048.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21213..22394)
                     /locus_tag="ACG8N9_000418"
     CDS             complement(21213..22394)
                     /locus_tag="ACG8N9_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4896049.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22630..24138)
                     /locus_tag="ACG8N9_000419"
     CDS             complement(22630..24138)
                     /locus_tag="ACG8N9_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4896050.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24297..24488
                     /locus_tag="ACG8N9_000420"
     CDS             24297..24488
                     /locus_tag="ACG8N9_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896051.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24691..25608
                     /locus_tag="ACG8N9_000421"
     CDS             24691..25608
                     /locus_tag="ACG8N9_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4896052.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25601..26674
                     /locus_tag="ACG8N9_000422"
     CDS             25601..26674
                     /locus_tag="ACG8N9_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896053.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26822..27340
                     /locus_tag="ACG8N9_000423"
     CDS             26822..27340
                     /locus_tag="ACG8N9_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896054.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27321..27713
                     /locus_tag="ACG8N9_000424"
     CDS             27321..27713
                     /locus_tag="ACG8N9_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4896055.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27789..28724
                     /locus_tag="ACG8N9_000425"
     CDS             27789..28724
                     /locus_tag="ACG8N9_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4896056.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28857..29636
                     /locus_tag="ACG8N9_000426"
     CDS             28857..29636
                     /locus_tag="ACG8N9_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4896057.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29629..29946
                     /locus_tag="ACG8N9_000427"
     CDS             29629..29946
                     /locus_tag="ACG8N9_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4896058.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30190..31800
                     /locus_tag="ACG8N9_000428"
     CDS             30190..31800
                     /locus_tag="ACG8N9_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4896059.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31957..32805
                     /locus_tag="ACG8N9_000429"
     CDS             31957..32805
                     /locus_tag="ACG8N9_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4896060.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33036..33515
                     /locus_tag="ACG8N9_000430"
     CDS             33036..33515
                     /locus_tag="ACG8N9_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4896061.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33548..35776
                     /locus_tag="ACG8N9_000431"
     CDS             33548..35776
                     /locus_tag="ACG8N9_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4896062.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35905..37548
                     /locus_tag="ACG8N9_000432"
     CDS             35905..37548
                     /locus_tag="ACG8N9_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4896063.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(37621..38166)
                     /locus_tag="ACG8N9_000433"
     CDS             complement(37621..38166)
                     /locus_tag="ACG8N9_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4896064.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38189..38674)
                     /locus_tag="ACG8N9_000434"
     CDS             complement(38189..38674)
                     /locus_tag="ACG8N9_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4896065.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(38664..39476)
                     /locus_tag="ACG8N9_000435"
     CDS             complement(38664..39476)
                     /locus_tag="ACG8N9_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4896066.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(39615..>40726)
                     /locus_tag="ACG8N9_000436"
     CDS             complement(39615..>40726)
                     /locus_tag="ACG8N9_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4896067.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13519 a         7388 c         7970 g        11849 t
ORIGIN      
        1 cagtaaacat ataggtagaa tctctactat cttatttctt gcatacaaaa aaatcaagga
       61 gtgagagagt tatgagatat tcaaccatct taatatcaac atttgcagcc ttaagcttat
      121 cgcttactat aaatcagaca gtccacacag aggagataga aacagccaaa tggacagaaa
      181 ccaagtgggc caacgtcaat cgaattacct ttcaggatga attttcatcc ttcaattcag
      241 gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa agcaaaaatt gactattcag
      301 gagacttagc acctatacta caatcagctg ttacatttga gaatgttgat cccgctttgt
      361 cgattggcta tgacgagaac actttgacta tccaacacaa gcaagcgata tttaccttca
      421 ccgttacatt aaatcaggac ttgttaaaac cagcgctttt tactgtcaag gtatcagatg
      481 gactaccgaa cgatcatcat catttaacgc cgtatagtca gcggcagaca attgagcaag
      541 attcagctat cgactcagga ggagatcttg ttgaagaacc aacagataaa ccagaaaatg
      601 aaaataagcc agaagtaccg cctacagaaa atccagacgg agaacagaaa ccggaaatag
      661 aacctggaga agaacccgat acagaaactc aaccagagcc agacaatgaa tcaaaacctg
      721 aaataacccc tggagaaaaa ccagatgtag acccagaaga aaaaccagat gtgactccag
      781 aaccagatac ggattctgga aaccaaacag taccggaaac aaatccagac acagataatg
      841 aaactgaaaa tccagaaaaa ccagaggtag atccagaaga aaaaccagat gtgactccag
      901 aaccagatac ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa aaaacaattc
      961 aagaggatgg aaaaaaagag agtaaaaaat caaatctagc aattctaaaa ataaatgaag
     1021 agcagctgaa taaaaaatca cgaatatttg atagcgccca atcagctgaa accttaaaaa
     1081 gtagcaagga tacaaccttt gctgtcctga aacgaaaaat aaacagctac caaaatccgg
     1141 agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta tagcaacaat
     1201 gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata atgtctgtga
     1261 acgcattttt caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc agattgaaat
     1321 ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag ggatatctgt
     1381 ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa agaaggtaaa
     1441 gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa caagtaagac
     1501 cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg gattcagcac
     1561 ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa catctcggat
     1621 gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa cacctgctac
     1681 tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat gaaagccaaa
     1741 tacagccaag gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa attgagcttc
     1801 taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat cttgaaagaa
     1861 ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt tcttttcaat
     1921 cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt ttcctatatc
     1981 ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa aatcgtttcg
     2041 ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct gatgctcgta
     2101 aaaattaata acggactgga tcatgtcaaa tgtttgatga ataagcatcg ggatatactt
     2161 tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag ctaaaacacc
     2221 tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca aataagtaaa
     2281 gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa caaaaacgac
     2341 aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct gataaacggt
     2401 caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat gatggaatat
     2461 gaaaataaga aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg agttggtttt
     2521 cctcataaaa tcgttcatct agcagtttca gccctttttc tggtcttgat gaaaattatc
     2581 tagcattttt cgggtcagat gttgcagtgt atccaattct tcctgttcaa gtggaagata
     2641 agacttgatc ttttgtggaa taccttctgc ttgtttttgc aacgcagcac cttgagcagt
     2701 cagcgtaaca tttacgattc tttcatcacc cgtttcacgc tgacgattaa tataattttt
     2761 ttctgctaac ttttttagta agggagtcag cgtaccagaa tccagacaca aatcatttcc
     2821 tagctctttc actgaagtct ttttcttttc ccataaaact aacatcacaa gatactgggt
     2881 ataggtaaga ttcagcggct tgaggagggg agtgtagtgt ctaatgattt ctttagaggc
     2941 ggcatataaa ggaaaacata cttgattaac taaatataga tcttccattt ttccacctcc
     3001 gctgttttac tatagtgtat catagcggat ttacacaaaa aagtctattc actaactgct
     3061 ttcagagtaa cttctatatt tccagctgta gcttttgaat acggacacac tttatgcgct
     3121 gcctctaata attcttgcgt ttcttccaga gaaagacctt cgatagatcc ttcaatctct
     3181 acacctaaga caaagctcgt ttcactttct gaataaaggg aaacttgtgc actgacagta
     3241 gaagccccag agatattttt ttgctgtttg atcaattcta atgcactgtt aaaacatgca
     3301 ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg tttaccagga
     3361 gctgcaatat tcaattcgaa tgtacgatcg ggattatgca cttctccaga gcgtccgcct
     3421 gtgttgatca ttttcgtaga atacattttt ttcataaaat taccaccttt aattatattt
     3481 tatacaattg aattttacac aatataattg tggaaagcaa agaaatggat tggcataaga
     3541 taaataagag aaagcacatg aaacagacgc gctttctcga atagcaaaca tatacaaaaa
     3601 atatcagaga caaaaaataa tctcatcata aacattacat atatttctca aaaagttgtt
     3661 ttctttcttc aaaaattact tcacgatgga cctgatgaag catccataaa taaccaaaag
     3721 gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct
     3781 ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat
     3841 tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt
     3901 catccagcat atggaatctc gtaccataca aggtaaaaat gacttcatta ttgcctctag
     3961 ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg
     4021 ctttcaaact atcatttaat acaaaatcga tttccgttcc tttatgcata aagatctcct
     4081 ctccttttca gtcgaacagt tactagattc ttttattata ccagctaaag aaaacgcatt
     4141 caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc
     4201 ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca
     4261 atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact
     4321 ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta
     4381 agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat
     4441 tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat
     4501 aaagaaactc ttctattcca aaaataaaca gattaagaga aacaacgaat gcgtctgtaa
     4561 aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg
     4621 tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg
     4681 cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg
     4741 caatgattaa aggaaaaacc aagcttccga aaagtacttt aaaaaagggt tttttaccta
     4801 aaaatagaag agctagtacc aacataacga tattcaacac taaaataact atagctcggt
     4861 caaagcctaa agcagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat
     4921 gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct
     4981 taatgtatgt ccatataaat tgtctcgttt ctttcattta ttcacttcct taattcatta
     5041 ataatactct tatcttatta taacttactt ttcaccttaa gttaacttta attaataaaa
     5101 aaattaggct agtctaaatt attatgtttc attcttctag aatatacttc tacgccaaaa
     5161 actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag ataaattcag
     5221 gacactaaat agtacaggaa gtagactaaa acttttaaaa gttttaagtc tatctatttg
     5281 gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca tttttgaaag
     5341 cgttgtcttt tgcttctcta tcacttatag tgatgacgtc atattaaaac acaaggagga
     5401 aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca tttctatata
     5461 cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg
     5521 tgctatgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa
     5581 tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg acgagcgaaa
     5641 caagtcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac tgtttttacg
     5701 gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat ttttattttc
     5761 gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta aaagaaacag
     5821 tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat gaccccgaac
     5881 acactttacc ttggggaagt aaagatccag accgagaaga ctttttcggg ggagatttgc
     5941 aaggtgtgat ggatcatttg gattacttga cggaccttgg catcaatggc atctattttt
     6001 gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat tatgaggtgg
     6061 atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca cataaaagag
     6121 ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca ccacagtggc
     6181 aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac atccgttttt
     6241 tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat atcccatatg
     6301 atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca gaagtgcaaa
     6361 aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat ggatggcgtc
     6421 ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa gcagttacag
     6481 aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa gcttggctgc
     6541 aaggggatga attccatgca gtgatgaatt atgcatttac agattcgatt aaagattatt
     6601 ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc
     6661 tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat gatactgcca
     6721 ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt
     6781 ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg actggtgaag
     6841 atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat ctagagctat
     6901 tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt atttctgaag
     6961 gttctactca gtggctaata gtaaatgatc gtgaagataa gctctatttc acacggaaac
     7021 tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg gtagtagaac
     7081 aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg aaagcagaga
     7141 tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca acaagaaagc
     7201 gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt tgaatacaaa
     7261 ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt taatctcttt
     7321 aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag aaggggaagc
     7381 cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt atataaacga
     7441 aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag aaaaggacat
     7501 gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac ctgacgttat
     7561 gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa
     7621 actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt atgatggaaa
     7681 aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca aagatttagt
     7741 cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca gtcgttttgc
     7801 ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg acttttatta
     7861 ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc
     7921 ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg caacagattg
     7981 gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag acttcgccag
     8041 ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc aagcgcaaac
     8101 atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa ataatggaca
     8161 gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa
     8221 agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag aaaaattctt
     8281 tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa
     8341 cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga tgccaaacat
     8401 cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg cagcatcagg
     8461 ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg aagccataga
     8521 acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa caggtgcttc
     8581 agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca ttatcttaaa
     8641 agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga taaaacaagc
     8701 aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg gcagtttgta
     8761 aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt cattggtcag
     8821 atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag tataccaatg
     8881 gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat tacaatcatt
     8941 ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa
     9001 gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg tgatgaaggt
     9061 tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat cattttccca
     9121 gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat tccaccagcc
     9181 agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt aagctcatat
     9241 cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg tgcaacaact
     9301 ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt tattaaattc
     9361 aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt tattactatc
     9421 atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca agtgattcct
     9481 ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac ggacccgaac
     9541 tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta tatctatgtg
     9601 atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc aaaaatcgac
     9661 ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt cctagtggca
     9721 gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat gatctatctg
     9781 tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga tattcttatt
     9841 tcctggatct ataaacttac gactggaact tctccgcagt attctatggc ttcagcaatc
     9901 acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa aaagacaaat
     9961 gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga ttccaaaaac
    10021 gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt atcatctatc
    10081 cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct tttagtttag
    10141 atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa acactttacg
    10201 ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta caagtgactg
    10261 tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc aaatcaagtt
    10321 tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact gccttttatg
    10381 tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg atctatatag
    10441 gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact gtaccaatag
    10501 atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt ttccagattg
    10561 tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt atgggacctt
    10621 ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag actgttgctg
    10681 tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt tttgctgctg
    10741 gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa aagaactttg
    10801 ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg caaacatttc
    10861 cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga cgcaaactat
    10921 tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg gtcatgcctg
    10981 tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt ctatccgttc
    11041 attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta tctgaaactg
    11101 ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta ggtgtgtctt
    11161 tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag gaacaatggg
    11221 tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat agcccatctt
    11281 tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa agagaatttt
    11341 atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt cttgtcctat
    11401 ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg acaagaaaaa
    11461 gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg aatgttatgg
    11521 gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt atcttgatgc
    11581 taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc aaaacaagat
    11641 tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa
    11701 aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt
    11761 cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa atcatgttag
    11821 agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc gaagtaaatt
    11881 attgggacgg tacaacgaaa caatacggag atacggacac tcctccccaa atcaaaatca
    11941 atatccatga agaaatccca atgaaagaga ttacaagtaa cgcttctttg gcactaggag
    12001 aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg atcaacgatg
    12061 cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg ctgccaaaga
    12121 aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat gacttaggta
    12181 acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct tatttcaata
    12241 cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc ttagacaaac
    12301 tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga acattgatgt
    12361 tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt gaagaacaat
    12421 acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc cgtgtattat
    12481 taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt
    12541 tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa gatttattga
    12601 aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt gcgacaaatg
    12661 cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact gaattagttg
    12721 ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt cgtgactacc
    12781 aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag
    12841 aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt gcagcttcat
    12901 ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga aacaatgaca
    12961 tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt taaaaagaag
    13021 ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga ttgtctctaa
    13081 agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta atttttaaac
    13141 atgacagaaa attttttctt cgttatactt gtaatagaat actattttat aacaaagggg
    13201 atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt gtttttagct
    13261 gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag tagcgaacag
    13321 cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc tagccagaca
    13381 aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc
    13441 gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt agatagtgat
    13501 tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga
    13561 attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga tcgagatgaa
    13621 caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat cacgattgag
    13681 gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga ttgggatttg
    13741 gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca
    13801 tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa
    13861 tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga caaaataact
    13921 tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat atctttgcca
    13981 atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta gatgaactgg
    14041 ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa cgaaaaggaa
    14101 atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg ggacttccaa
    14161 acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc
    14221 aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc
    14281 ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta tcttgtccca
    14341 atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa acactgaaag
    14401 aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat tttgattttg
    14461 ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat gtcaatgcta
    14521 tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta gtcatcaaac
    14581 caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct ttggcgaatg
    14641 ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca ggtgggattc
    14701 ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta cagattggta
    14761 ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa ttaacgcaga
    14821 ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta cgcaccattt
    14881 catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa aacactcgtc
    14941 tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg aaaataacag
    15001 aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa
    15061 agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga agcaaaatca
    15121 tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg agctattgat
    15181 tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa tgctcggtca
    15241 agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac accgagcttc
    15301 tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt ttggacaaac
    15361 caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg gcccagcaag
    15421 aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg ctgctgcagt
    15481 ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta ttagaatacc
    15541 cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc cagttggtcc
    15601 atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag tttctatgag
    15661 agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc catatcaagt
    15721 atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt
    15781 tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa aaaaagctgt
    15841 agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac tagatggtcg
    15901 taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa tgatccgctc
    15961 gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca tttcttggac
    16021 tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc tgcatgtatg
    16081 ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat tcctttcacc
    16141 aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg cgataagagc
    16201 acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg tttcttccat
    16261 gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga ctggacaacc
    16321 agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa tccaatttat
    16381 ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg aagcaaatca
    16441 tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag aatgaagaaa
    16501 aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg cggtacagct
    16561 gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga attagtagta
    16621 gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga attagttggt
    16681 tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat ctcctttaaa
    16741 gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg
    16801 aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga gactttcaaa
    16861 tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa
    16921 gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg tttagcggaa
    16981 aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta tttaagcgat
    17041 gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa aatggccgta
    17101 tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa
    17161 agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga tggcgtatta
    17221 aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt tcaaactgat
    17281 gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg tactttcctc
    17341 taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc ttggtataca
    17401 cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg cttttgacag
    17461 ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat ttatcgttct
    17521 ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta ccttatcaat
    17581 taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca tttattggtg
    17641 aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct ggtttagctt
    17701 ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct tttcgaatct
    17761 cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg ttagcagcaa
    17821 caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg acattcgatt
    17881 atttttctct ctatatttta gttgcactga tttattgggt catttgtatc gggatcgaaa
    17941 aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa atagaaatgg
    18001 tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc ctagtatttt
    18061 aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac
    18121 cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc aaggcggctt
    18181 ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc ttagctgctt
    18241 gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga caaaagcaga
    18301 ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg ttttaccaag
    18361 taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt tagtgtatcg
    18421 tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg cgaaaaaagc
    18481 agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca tggcttcttt
    18541 attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt ttccaacaga
    18601 tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt ctaacgaacg
    18661 atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt tgcaagaaga
    18721 agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga tccgtcggtt
    18781 gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg aaaacaattt
    18841 gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa aatccaacca
    18901 gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa
    18961 aagcaccatc caatcgctgt gatccaaaat ccccaagtag gacaattcaa attattcgat
    19021 cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc acagatctta
    19081 acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt tttcgatgga
    19141 cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga ttcgaaattt
    19201 ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt
    19261 gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga tgactttttt
    19321 agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga tttcacgatc
    19381 gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat ccatctaacg
    19441 tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag taacgactct
    19501 gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa atggatcatg
    19561 cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc tctttaccag
    19621 caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca tcatttggaa
    19681 cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc aaaagttcag
    19741 gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat agaaaagagg
    19801 aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg tagaaatcat
    19861 taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa acgggcagaa
    19921 accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg tattaatagt
    19981 ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg tttacacaaa
    20041 aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc gtgtcgcttt
    20101 agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt tgttgacgat
    20161 tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta atgaaccgat
    20221 ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat ttccttttga
    20281 acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc atctgagcaa
    20341 agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca
    20401 gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca aatttttgtg
    20461 gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc gagaggtcgt
    20521 gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga tacatttata
    20581 tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg tttgacacga
    20641 ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct acataaaccg
    20701 ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc gattgcaatg
    20761 tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac tcatataaat
    20821 gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt ttctttttaa
    20881 tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct gcaaactcat
    20941 ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc tcttgatctt
    21001 tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa
    21061 tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca tagcaataat
    21121 tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa atatttccaa
    21181 agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt cacaatagca
    21241 cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt tgcgacaaga
    21301 tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat agaagagaag
    21361 ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt aaaatggaaa
    21421 ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt agtcgccatg
    21481 atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc aagaggggca
    21541 aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa gatagcattg
    21601 atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat cgccacatgg
    21661 aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt gttttcttca
    21721 atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga agccagaata
    21781 taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc gatcagtgcc
    21841 atataagatc cgacgatcga catagaaact gtagacatag ccgatacact catggtgaac
    21901 aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac ttccgattgt
    21961 cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc gttgatttta
    22021 cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata gcgtagaata
    22081 ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa aggtgcactt
    22141 cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga
    22201 tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt acgtaataat
    22261 acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa tttgattgct
    22321 tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc aataatacct
    22381 aataaataac tcaaataatc actcctattc caaattccgt tttattttac ctgttttgca
    22441 aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa cgcattcatt
    22501 ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa
    22561 gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt
    22621 aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc
    22681 gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc
    22741 ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac
    22801 tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg
    22861 aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat
    22921 gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc
    22981 catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac
    23041 agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg
    23101 gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg
    23161 attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg
    23221 acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc
    23281 tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt
    23341 ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac
    23401 tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt
    23461 ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt
    23521 ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa
    23581 ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt
    23641 tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac
    23701 tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt
    23761 gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa
    23821 taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc
    23881 ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc
    23941 agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag
    24001 tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat ataatttgtc
    24061 atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc
    24121 aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt
    24181 gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc
    24241 aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga
    24301 aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt
    24361 atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag
    24421 aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta
    24481 gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac
    24541 tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc
    24601 ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat taataagtga
    24661 aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat
    24721 gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga atcgtcttct
    24781 ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag
    24841 gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc
    24901 cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg
    24961 atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac
    25021 atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc
    25081 ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag
    25141 aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag
    25201 gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga
    25261 caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa
    25321 attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct
    25381 tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga
    25441 gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc
    25501 ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat
    25561 gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc
    25621 agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga
    25681 agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa
    25741 aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc
    25801 ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat
    25861 cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt
    25921 gatcgaatca gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga
    25981 accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa
    26041 accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag
    26101 acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt
    26161 accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga
    26221 gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt
    26281 ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt
    26341 agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc
    26401 acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt
    26461 tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca
    26521 agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt
    26581 cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga
    26641 attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc
    26701 attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc
    26761 ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg
    26821 aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag
    26881 gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt
    26941 actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag
    27001 tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct
    27061 gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg
    27121 agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct
    27181 gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat
    27241 gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta
    27301 caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca
    27361 gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg
    27421 aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt
    27481 cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt
    27541 ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga
    27601 aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag
    27661 agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat
    27721 aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg
    27781 aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg
    27841 ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca
    27901 tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg
    27961 gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa
    28021 aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg
    28081 tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg
    28141 ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga
    28201 aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc
    28261 gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct
    28321 tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca
    28381 gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt
    28441 atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt
    28501 ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg
    28561 agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg
    28621 tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat
    28681 acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa
    28741 ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg
    28801 aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga
    28861 acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa
    28921 gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc
    28981 tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat
    29041 ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat
    29101 tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt
    29161 ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt
    29221 tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga
    29281 ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg
    29341 ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga
    29401 aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg
    29461 tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat
    29521 ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag
    29581 atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta
    29641 tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg
    29701 atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta
    29761 ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg
    29821 gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa
    29881 acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg
    29941 ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc
    30001 tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa
    30061 agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg
    30121 tccttctatg attttttgct ataataggga agatgtttat tttatgaaag aatagttgag
    30181 ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt
    30241 ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat
    30301 caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc
    30361 ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca
    30421 aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt
    30481 gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc
    30541 agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg
    30601 attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg
    30661 ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt
    30721 acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca
    30781 gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga
    30841 aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac
    30901 cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc
    30961 attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt
    31021 ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat
    31081 aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata
    31141 ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt
    31201 tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca
    31261 ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg
    31321 ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat
    31381 caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa
    31441 gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc
    31501 ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta
    31561 gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta
    31621 tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca
    31681 atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc
    31741 ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag
    31801 gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc
    31861 gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat agttataagg
    31921 tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat
    31981 agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc
    32041 cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat
    32101 tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa
    32161 gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat
    32221 ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc
    32281 ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg acacatggta
    32341 gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc
    32401 gatcgaatcc gagataatac ctacgcttat ctgattgtag atgatttgaa caatttagta
    32461 cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg
    32521 atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa
    32581 gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc
    32641 aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg
    32701 cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca
    32761 catttaggtg aaaaagccat cggcattgcc atttcagcgc aataattcct tttattcaaa
    32821 aaacatacca aaaatattcc aacatcatct tggactttct acatgttact aatgagcgtg
    32881 taaaagaacg agatggtgtt tttatttcta tcaatgaagc tctttttctt tgcaatcctc
    32941 acttcaatcc tctataatgt cgcatatagt ttagtaaatg agtaaactac taatttactt
    33001 atttactaaa aataaaagga tgaggagtta aaataatgga aataccaaca aatttaaaac
    33061 ataaaccagt aatcgttgta gaagactatg ataaagttga tggaagaaat gcattaaata
    33121 cggatgcaaa agggctttca ctgggccttg ctcagtggaa tgatcgtgga aaagtcgata
    33181 tctcaggcaa ggtatggcga catactggag aaaaatggtc acgacagtcg gaagaattac
    33241 caatcacacg tattttagat ttagcgatct tagctgcaca aggaagttta tacttccaag
    33301 atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc agtcgtagac atcattggat
    33361 tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa tccaaagatc gatgaagaca
    33421 tcattttgta ttacgataca ctacaaaaag acggagaact gattggggaa cgattccgta
    33481 ttttaaaacg tcttttagat gagctaggat attaatagac agagatgatc aaaaggggaa
    33541 gagaagcatg gagaatttct cactaacaga aaaccgaacc ttttcacggc tagaaaagca
    33601 aatgatctgg aagaaaccga aaagccatcg gacaagtgca gaagaatggc ggatcgcctc
    33661 cgaaatcaaa gggaattggc aagatcctga aatgaaaatc agtaacgtac ttttggaagg
    33721 agatgccggg tcggggaaga cacaattagc caaagcacta tctgctgatc tgcaacttcc
    33781 ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca gatgttttcg gagccttatt
    33841 acctgtagta gattctaata aggaacaaga ccaagaatta ctagaagcta tttaccaaac
    33901 agatacactt gaagctgtac tgacactgat ccagaaccat tacggcattg atcctttttc
    33961 tgctaaagaa aaattagcag agctagttca aaggatcgaa gctgattatc cagatgcaat
    34021 ccattacagc tattaccctt ctgaaatcgt tcgagcgatt gaaaaaggat atttattaga
    34081 gattcaagag ccgactgtca tccgagatgc ctctgttctt gtggcactta actcagcgct
    34141 cgaaccaaat gggatgttga atttaccgac aggaatcatt cgtcgccatc cagattgcgt
    34201 cattgtgatc actacaaatc gaaattacca aggaaatcgc ccattaaatg aatctttaag
    34261 agatcggatg caacatgcgg aaaaaatgga tcttcctgcc ttagaagtca tggcagaacg
    34321 tgcgatggcg aaaactatga tccaaaaaca agaacttctc ttaaagatgg cagagattat
    34381 ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa ggtgtagcag gaatgcgttc
    34441 ttatttttat tgggcaaata cctataagca aggacaagat ttactccagt cgatttatcc
    34501 aaaagttttg tataaattat ccactgattc agatgaattg gcaattcttg aacaagcttt
    34561 gtctgacagt ggattgctac tggaacttca ggacatattg agaaaagaaa aatggggtca
    34621 tcctcaatct gagcatacaa aaggaagaac cattagcgcc gaagaagcaa atgaacgaaa
    34681 catcactgaa aatgctgaag aaattctgcg cttagatgaa aagaaatcgt tgtctgctga
    34741 agaaccaaat gagggagaag agaaacaaga agaaacaata gaaaaaaacg agattcctcc
    34801 tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa aatcaaaaag aacaacaaac
    34861 aggagatgat tctcaagaaa tgtctgcttc tgacatggaa gcacatgata aggaaatcaa
    34921 gaagcaactg aataaagaag cgcgacaaat catgaaggaa acgattcatg aaaaagaagg
    34981 tctcatcgtt caccgaccag ctttccaagg aaaaacgccg gaagcacgtg agctacacat
    35041 gcaagtaatg ccaatagtag aatcacttag ccggcaaatt ttagagttac tggataatga
    35101 acaaacagag acgtaccaaa aaggaaaata cgaaggtcag cggttcaatg caagcaaagt
    35161 agcatatggt gatttgagaa attttgacaa aaagaacccg ccacatgagc aaccttcttt
    35221 agcggtagct gtacggatcg atgaatctgg gtcaatgata cgggatgatc ggatcgaagc
    35281 ggcaaaaaaa gcagcgattg ccattgccga attttctaag aaagtcgata tcccattgtt
    35341 gatttacgga gatacagcag accgttcgtc aagagaaaag acttctttgt tttcttataa
    35401 agaatttgaa gacggatttc aatggttaaa tgaaaaatta gtcactatga aaccaaggca
    35461 aaacaatcgt gatggtgctg cacttcgttt agcagcagaa aaattaaaca gacaatccgc
    35521 aacaaccaaa ctgttgctta atattagtga cggtcagcca aaagcgttgc cggattatac
    35581 aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa gaatatgaac gtcaaggaat
    35641 cctatttgtc tccgcagcta ttggccaaga caaagaagaa atcaagtcga tctacggtga
    35701 aagtcgtttt gtagatatta ctgacttgaa tgaatttccc aagcaaatga ttcaactgat
    35761 tgcccgctat ctatgataaa ataaagctca acgaacgcac ttagcagatt cgttgagctt
    35821 ttcgtttcta gttaccggta acaaggatac ttctaaaata gaatcggtta taatcaagag
    35881 ggaccgttaa aggagtgaaa cgatatgata caagaacaaa aatggtggaa agaagcagta
    35941 ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg gcgatggtca aggagacatc
    36001 aatgggatcc gtgaaaaact taattatttg aaagatcttg gtatcggctt tatctggatc
    36061 aatccgatct atcaatctcc atttgtcgat aatggttatg acgtaagtga ctatcaagat
    36121 atagaagaga gattcggaac gatggaagat tttgatttat tgctaaaaga agcacatgaa
    36181 ttagggatca aagtgattat ggatcttgtc atcaaccata gttcggatca gcatcaatgg
    36241 tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg attattacat ttgggtcgac
    36301 ggggtagatg gcaaagaacc gaataattgg acatcgattt tcggcggttc tgcttgggag
    36361 tattctcatg aaactggaca gtattatctg catgtttttg caaaagaaca accagactta
    36421 aattgggaaa gcgagaaatt gaaagaagaa ctattcaata tgatccgatg gtggttggat
    36481 aaagggatag atggatttcg tttagatgcg atttctcatg taaaaaaaga tgaatattca
    36541 gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg tctcaggaat cgaagaacat
    36601 ttgaccgatt tgaaacatgt ttttgaagag tacgatatca tgactgtcgg agaagcaagt
    36661 ggtgtcactg cagaagaagg accacaatgg gtagggaaag acggttattt cgatatgata
    36721 ttcgaatttg atcatatcca tatctggcag caagaaaaag aagggcaatt agatgtcctt
    36781 aaattgaaac ttgcattaag cgcttggcaa acttcattgg atggtatcgg atggaacgct
    36841 ttatatatgg aaaaccatga tgttcctcgt gccgtttcag tgtttggaga tactcgtccc
    36901 gatttttggg ctatgtctgc aaaagcaatc gctatgatgt atttcttttt gcaaggtacg
    36961 ccatttattt atcaaggaca ggaaatcgga atgacgaata tgccctttga atcgatcgat
    37021 caagttgatg cagtagattc gaaaagactt tataaaaggc tgcttgcgga aggtaagaca
    37081 agagaagaag cattggatat cattcgtgaa acaacgagag acaacagcag gacacccatg
    37141 caatggactt cggaacaata tgctggtttt tctacacatg aaccttggct gatcactaat
    37201 ccaaatacca aaacgatcaa tgtagaacaa caagaatatg aaccagagtc ggtcctgcaa
    37261 ttctataaga acatgatccg cattagacaa accaataaag gtctgatata cggttcttat
    37321 aaagaatatc ttcatgaaca tccacaactt tatgtctatg aaagatattt agaagatgaa
    37381 caatatctta ttatggtaaa cttaacagaa tcacttgctg actatgaact tccaaaagaa
    37441 gcagatcagt cctggacatt actcttgtca aattcctcta gcggagaatt tgaagctaaa
    37501 ggcatactag caccttatga agctcggtta tataaaacaa acaagtaaaa cttcctcgtt
    37561 aaagttcatt agttggactt tgcgaggaag tttttcagat tgctctgtca attactcatt
    37621 tcaataattc gctctaggac caccgatata tttaggacga gagcgaaggg ctgtcacgtg
    37681 agcttgtcca agggttttca aacgaggtct gacaggtact tggacatgtt tgatatgcat
    37741 accgattgat gtgtcaccga tatcgatacc ggcttgtcca actacgtgtt ccacttcgac
    37801 aggatcattg aactggtcaa aagcagcaac agaacaagca cctccagcat gtagtgcagg
    37861 aacaacagag acaatttcga aattttttgc ttctgccact gttcgttcta ctactaatgc
    37921 tcggttcaaa tgttcacaac cttggacagc tagcgcaatt tctttaggat ccaacaactc
    37981 ttttaacgta cggatgatcc attgaccaac ttccgcactg gaattttttc caatatgtcc
    38041 tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga tcaccttttt tcaagtgtgc
    38101 ctcagccaat atgtcattaa ctatttcagt caattggtct tttaattctt tttctgacaa
    38161 cttcattttc taaaaccttc tttttcaatt aatattgctt ccgtgcttga atacgttgaa
    38221 gacttgcagt tataggaaag gcaagtccca ttccaatcag attttggaag atgtttcctg
    38281 gtatagaagc cactccagcc ggccaatcat ataaaatcca accggcgata ccatagccga
    38341 tgatcataac taagcttcct aataaaagcc ctaatcccca catacgagta gaagatttgc
    38401 tgaaagaacc agcaacatat ccttgtaacc cgtggatcac taatgaaaaa actgcccact
    38461 gcggatatcc tgaaatcaaa tcgatcaata gaccactgga agcaccaaca actaatccac
    38521 ccgttggacc ataaagaaaa gcagcggtat agatcccggc ctcgcataat gtcacaaatc
    38581 cattcgtagc cggtacaggg atcaaaattg ttaatgaaag ggcaactgtc aacgcaatca
    38641 gcatagcata gatcgttaat tgcttagttt tcatttctca ttcctccttt ttgaataccg
    38701 ccagaccaga cacttccggt atgatcgttc atcagcacgc cattagaaat acaatgggca
    38761 acaaattttt tactttctgc tactgctttg gtaaaatctg cccctaacac aagattcgct
    38821 gttattgcag aagaaaaggc acaaccggcc ccgtttactg ttgtagtgtc gattttttta
    38881 tgagaaaata cctcataatg atcaattgaa tagagaagat ccacagcttc atctccagcc
    38941 atccttgtgc cgcctttgat cacaatgttt tttgttcctg aagcagtgag ttctttagcg
    39001 agatggatca tttctgttaa cgaagaggga agagcttgac cagaaagtaa agcagcttct
    39061 gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt cttcgatgag ctgcgaaata
    39121 tacaattgat tgatcacagc ttcagtttct ttgaatgcca aaacagggtc taaaatgatt
    39181 gggatatttt gttttgtttt tatgaagtct cgaacaacca tgattgcttc acttgaattc
    39241 aataacccaa ttttgatacc atctaatgaa gtcattttat caatcgaaac taactgtcgt
    39301 ttaagcaatt ccaaagatat agattccagc ataaatcgct cattaatatc tagtgcccct
    39361 atagaagtca aggcgcttag tccgaataga ccaaaattct caaaagtttt taaatctgtt
    39421 tggattcctc caccagcaaa tggatcagaa ccgccaattg tcaagattct tttcataaca
    39481 ttttcctcca tgaataagag tataacgaaa ttttcaaaaa agaaaaacag ccaattggca
    39541 tatttttatc tcatccaatt taaaaaaaca aaaagaaaag aaagaccaaa atggatcttt
    39601 ctttccttaa atagttagga cttttgtgat tttaattctt tctcgatttg aagccgttgc
    39661 actttcattg tagctgtccg cgggattgca tcaaaatcac ggatgatagg gacgttcaag
    39721 cgaggaagtt catggacttg ctcccaccat cgattccaat ccatttcttt atcaggagct
    39781 aaagcaataa tcggttgtgg agaatgattt ttatccctta caatcactac ttctgccaaa
    39841 aaatctaacg aatctaataa aaagtcttca atagctaatg tactattgat cgtttcgatc
    39901 aaatccactt gccgatcttt caaaaatagg tggccatgct tatccataaa accgtaatct
    39961 ccgctatccc accaatcgcc ataaacattt tcctggaacc gtgcatcttc cttgtaataa
    40021 gtcaaagccc gtcctttaga cagtaattgt atatgaccat ctgtgttttc aggtaggaca
    40081 ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt ccagtccaac gcccatatct
    40141 cttgcatcac ttgttttcaa actctctagt gtatgagcct taagaatcat tggtccgcat
    40201 tcactttggc catatacttg aagaaatacc gcatcctgtg ccatcgacgt tcgcaaaaat
    40261 gtcttcattg tctggttgtt tatagcatca aaagtggagt gatagtatcg gatacccgaa
    40321 aaagcttctg gatgctcctt agcagtaaaa cgccactgaa caaagttatt cggatgggtc
    40381 tctagtgcaa ttggccggtg agcttccaac atatgaacaa cttttgaact ttgtgcgttt
    40441 gctaaaggca tcataggaaa tcccatggac atcaaagaag agataccaat attgaatctg
    40501 gaatgcactg gagagatgtg aaaagcgatt aatttctttt ccgaaatttt tgtgaaaact
    40561 gttttttgcc atttcgtccg ccatcccata gaatgagcag aatgacaaat aagtttaggg
    40621 ataccagttg taccagaagt atgggtcata taagaaattt catttttgtc taaaaatgat
    40681 tgagaaacag acaaagcagg ttgttccatt aaataaagaa taggaa
//
LOCUS       DAZUKD010000010        39647 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000010 DAZUKD010000000
VERSION     DAZUKD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.010"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..603)
                     /locus_tag="ACG8N9_000437"
     CDS             complement(58..603)
                     /locus_tag="ACG8N9_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4896068.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(615..1307)
                     /locus_tag="ACG8N9_000438"
     CDS             complement(615..1307)
                     /locus_tag="ACG8N9_000438"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4896069.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1326..1610)
                     /gene="macP"
                     /locus_tag="ACG8N9_000439"
     CDS             complement(1326..1610)
                     /gene="macP"
                     /locus_tag="ACG8N9_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4896070.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1623..2186)
                     /locus_tag="ACG8N9_000440"
     CDS             complement(1623..2186)
                     /locus_tag="ACG8N9_000440"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4896071.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2414..3076
                     /locus_tag="ACG8N9_000441"
     CDS             2414..3076
                     /locus_tag="ACG8N9_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4896072.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3200..4396
                     /locus_tag="ACG8N9_000442"
     CDS             3200..4396
                     /locus_tag="ACG8N9_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4896073.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4497..5873)
                     /locus_tag="ACG8N9_000443"
     CDS             complement(4497..5873)
                     /locus_tag="ACG8N9_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4896074.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5910..6776)
                     /locus_tag="ACG8N9_000444"
     CDS             complement(5910..6776)
                     /locus_tag="ACG8N9_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4896075.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6865..7890)
                     /locus_tag="ACG8N9_000445"
     CDS             complement(6865..7890)
                     /locus_tag="ACG8N9_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4896076.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7908..8582)
                     /locus_tag="ACG8N9_000446"
     CDS             complement(7908..8582)
                     /locus_tag="ACG8N9_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4896077.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8766..9659)
                     /locus_tag="ACG8N9_000447"
     CDS             complement(8766..9659)
                     /locus_tag="ACG8N9_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4896078.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9733..11430)
                     /locus_tag="ACG8N9_000448"
     CDS             complement(9733..11430)
                     /locus_tag="ACG8N9_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4896079.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11423..12736)
                     /locus_tag="ACG8N9_000449"
     CDS             complement(11423..12736)
                     /locus_tag="ACG8N9_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4896080.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12736..16677)
                     /locus_tag="ACG8N9_000450"
     CDS             complement(12736..16677)
                     /locus_tag="ACG8N9_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4896081.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16786..17244)
                     /locus_tag="ACG8N9_000451"
     CDS             complement(16786..17244)
                     /locus_tag="ACG8N9_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4896082.1"
                     /translation="MKGLKLKILQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17542..18060)
                     /locus_tag="ACG8N9_000452"
     CDS             complement(17542..18060)
                     /locus_tag="ACG8N9_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4896083.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18062..18679)
                     /locus_tag="ACG8N9_000453"
     CDS             complement(18062..18679)
                     /locus_tag="ACG8N9_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4896084.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18676..19464)
                     /locus_tag="ACG8N9_000454"
     CDS             complement(18676..19464)
                     /locus_tag="ACG8N9_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896085.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(19526..20437)
                     /locus_tag="ACG8N9_000455"
     CDS             complement(19526..20437)
                     /locus_tag="ACG8N9_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896086.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20478..21812)
                     /locus_tag="ACG8N9_000456"
     CDS             complement(20478..21812)
                     /locus_tag="ACG8N9_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4896087.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22020..22850
                     /locus_tag="ACG8N9_000457"
     CDS             22020..22850
                     /locus_tag="ACG8N9_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS4896088.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23094..23343)
                     /locus_tag="ACG8N9_000458"
                     /pseudo
     CDS             complement(23094..23343)
                     /locus_tag="ACG8N9_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23534..23728)
                     /locus_tag="ACG8N9_000459"
     CDS             complement(23534..23728)
                     /locus_tag="ACG8N9_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896089.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24233..24418)
                     /locus_tag="ACG8N9_000460"
     CDS             complement(24233..24418)
                     /locus_tag="ACG8N9_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896090.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25268..25609)
                     /locus_tag="ACG8N9_000461"
     CDS             complement(25268..25609)
                     /locus_tag="ACG8N9_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4896091.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25609..25839)
                     /locus_tag="ACG8N9_000462"
     CDS             complement(25609..25839)
                     /locus_tag="ACG8N9_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4896092.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26126..26464
                     /locus_tag="ACG8N9_000463"
     CDS             26126..26464
                     /locus_tag="ACG8N9_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896093.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26669..27231)
                     /locus_tag="ACG8N9_000464"
                     /pseudo
     CDS             complement(26669..27231)
                     /locus_tag="ACG8N9_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27963..28544)
                     /locus_tag="ACG8N9_000465"
     CDS             complement(27963..28544)
                     /locus_tag="ACG8N9_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4896094.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28727..29353)
                     /locus_tag="ACG8N9_000466"
     CDS             complement(28727..29353)
                     /locus_tag="ACG8N9_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4896095.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29375..30706)
                     /locus_tag="ACG8N9_000467"
     CDS             complement(29375..30706)
                     /locus_tag="ACG8N9_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4896096.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30861..31262)
                     /locus_tag="ACG8N9_000468"
     CDS             complement(30861..31262)
                     /locus_tag="ACG8N9_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4896097.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31348..31554)
                     /locus_tag="ACG8N9_000469"
     CDS             complement(31348..31554)
                     /locus_tag="ACG8N9_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896098.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31649..33151)
                     /locus_tag="ACG8N9_000470"
     CDS             complement(31649..33151)
                     /locus_tag="ACG8N9_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4896099.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33169..33624)
                     /gene="argR"
                     /locus_tag="ACG8N9_000471"
     CDS             complement(33169..33624)
                     /gene="argR"
                     /locus_tag="ACG8N9_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4896100.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33722..34339)
                     /locus_tag="ACG8N9_000472"
     CDS             complement(33722..34339)
                     /locus_tag="ACG8N9_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4896101.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34560..35933
                     /locus_tag="ACG8N9_000473"
     CDS             34560..35933
                     /locus_tag="ACG8N9_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4896102.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            36008..37120
                     /locus_tag="ACG8N9_000474"
     CDS             36008..37120
                     /locus_tag="ACG8N9_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4896103.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37192..38829)
                     /locus_tag="ACG8N9_000475"
     CDS             complement(37192..38829)
                     /locus_tag="ACG8N9_000475"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4896104.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38997..39605)
                     /locus_tag="ACG8N9_000476"
     CDS             complement(38997..39605)
                     /locus_tag="ACG8N9_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4896105.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11370 a         8439 c         6888 g        12950 t
ORIGIN      
        1 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
       61 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      121 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      181 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      241 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      301 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      361 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      421 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      481 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      541 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      601 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      661 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      721 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      781 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      841 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      901 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
      961 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1021 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1081 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1141 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1201 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1261 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1321 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1381 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1441 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1501 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1561 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1621 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1681 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1741 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1801 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1861 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1921 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     1981 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2041 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2101 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2161 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2221 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2281 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2341 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2401 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2461 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2521 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2581 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2641 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2701 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2761 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2821 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2881 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     2941 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3001 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3061 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3121 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3181 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3241 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3301 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3361 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3421 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3481 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3541 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3601 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3661 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3721 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3781 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3841 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3901 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     3961 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4021 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4081 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4141 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4201 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4261 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4321 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4381 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4441 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4501 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4561 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4621 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4681 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4741 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4801 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4861 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4921 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     4981 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5041 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5101 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5161 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5221 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5281 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5341 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5401 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5461 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5521 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5581 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5641 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5701 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5761 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5821 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5881 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     5941 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6001 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6061 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6121 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6181 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6241 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6301 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6361 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6421 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6481 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6541 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6601 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6661 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6721 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6781 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6841 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6901 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     6961 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7021 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7081 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7141 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7201 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7261 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7321 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7381 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7441 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7501 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7561 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7621 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7681 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7741 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7801 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7861 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7921 tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg
     7981 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8041 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8101 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8161 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8221 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8281 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8341 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8401 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8461 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8521 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8581 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8641 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8701 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8761 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8821 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8881 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     8941 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9001 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9061 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9121 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9181 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9241 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9301 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9361 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9421 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9481 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9541 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9601 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9661 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9721 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9781 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9841 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9901 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
     9961 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10021 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10081 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10141 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10201 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10261 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10321 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10381 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10441 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10501 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10561 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10621 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10681 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10741 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10801 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10861 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10921 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    10981 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11041 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11101 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11161 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11221 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11281 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11341 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11401 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11461 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11521 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11581 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11641 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11701 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11761 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11821 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11881 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    11941 cattagcagg agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa
    12001 aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga
    12061 agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt
    12121 cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct
    12181 gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa
    12241 ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc
    12301 cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg
    12361 tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag
    12421 gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa
    12481 atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct
    12541 cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc
    12601 gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca
    12661 tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac
    12721 acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata
    12781 ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga
    12841 tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat
    12901 tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc
    12961 ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac
    13021 ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg
    13081 aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa
    13141 gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat
    13201 ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg
    13261 gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa
    13321 ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc
    13381 ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac
    13441 ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat
    13501 agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg
    13561 agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc
    13621 agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc
    13681 accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa
    13741 cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag
    13801 atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta
    13861 aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct
    13921 ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga
    13981 atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc
    14041 ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga
    14101 aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc
    14161 aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg
    14221 gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc
    14281 ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt
    14341 cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc
    14401 gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt
    14461 ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct
    14521 gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc
    14581 cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata
    14641 taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa
    14701 attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt
    14761 gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa
    14821 atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc
    14881 ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt
    14941 cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg
    15001 ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga
    15061 tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg
    15121 gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt
    15181 gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa
    15241 tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa
    15301 accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata
    15361 agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt
    15421 attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc
    15481 ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac
    15541 tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa
    15601 aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt
    15661 agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc
    15721 cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt
    15781 ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa
    15841 tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga
    15901 actgatttgc tcatggttat tgatagctgt actattatgg taaaaattca ccataccgat
    15961 tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat
    16021 caacgcagca tttttagttt gactattggt cccagcggtt aattcactca tactcgctga
    16081 cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac
    16141 atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct
    16201 gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact
    16261 gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc
    16321 ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc
    16381 cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc
    16441 tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt
    16501 tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat agatatattc
    16561 ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga ataactggcg
    16621 ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct tttccatctc
    16681 attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat tttcttgtaa
    16741 ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    16801 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    16861 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    16921 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    16981 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    17041 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    17101 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    17161 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    17221 aatcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    17281 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    17341 tcaaatgata tcctttttag ttaaaaaaca ccaattatac aacatttatt attaaaatta
    17401 aattataaaa ttttataacg ttctcattta acaaaactta agttattaag acaaatgaat
    17461 aaacaactac gctttcctcg aaacaaaaaa ataaagcaca aatgcgcatt tacgcactca
    17521 tgctttattc gtacaattaa ttcagataac ggcagcgcat aggatgccaa taacacttcc
    17581 taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa agtaaaaaaa tcctcgaata
    17641 agcgatcaaa caagcaaata aaaaggctaa tattcctgca actggactta attcgaacaa
    17701 cgcagctact gataaaaagg ctagcgctgt atgtcctgaa ggaaaagaag aagtcttcgg
    17761 tttacttgtg taaaggacaa gccgacgatt tttttcataa ggtcttttcc gtcttgtcgt
    17821 ttttttgata aaaagcaaac caacaaaaaa atagatcaaa ctgccgataa gaatggctag
    17881 accatattct ctagtatcag gtatcaagac taaaacagca gcaaggagca accaaataaa
    17941 accaccatta gagatattac tgataaattt caaaaaattg atttttcgac tatccgtttt
    18001 ccgttgcttg gcgaacttgt tgataatact atcatcgatt ttagcaatca aacgcaacat
    18061 gttattcctc ctttttaaga accttttctt gcgtacgatg gataaaaagc aggacttgaa
    18121 aataaaacaa gaaactgaaa cctaataaag caaaatagat tggtcctgca aaccagaatg
    18181 cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc gcttttgaaa tcattaaaaa
    18241 acaagaaaaa tgcagtaatc caagaagctt ctttttgttc cagttgaagc aagattttgt
    18301 aactaaagag aagcgcgctt aatagaagca aaaacaacgt agtatacatc aatgtatagc
    18361 caattccccc accccaatag cggaaaatct gataatcgat caatagaaga agaaaataaa
    18421 cacttgctaa actatcttgc tttccataaa gagagaagtt ctcacgaaac tttttccaga
    18481 atagtgaacc gagctttttc tcttcatttt ttttgtaatg ataaatcata tacagaactg
    18541 ttcgacttgc agttcctaat gtgaaaacag gcaagacgca caggaaccat agtagctgta
    18601 aatggataag acgtggaatc cattcaatga tttctaagaa ataatcattg gcttttttac
    18661 caatcgatcg attcatcatc cttttgaacc tcctgtcaaa tccatccctt caatgaaata
    18721 acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag acaagtgttg ctcctgccat
    18781 cagataattc cattgtgtcg ttgcttggtt ctgaaaaact tgcaagccaa tctgtaacgt
    18841 gtatttttct tgatcttgga tatataacag aggacctaag aaatcattcc aagctgcgtt
    18901 gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc aatggaagca tcaaatgact
    18961 ccaaatatac aaatgattcg ctccgtcgat ttcagcagct tcgatcaatt cattattgat
    19021 cgataagtaa aattgacgca tcagaaatat attgaacgca ttgccgaaga acgatggaac
    19081 gatcaggggc aaataagtac ctacccagcc gatcttggag aataatacat attgagggat
    19141 catcgttaca aaccccggga tcatcatcgt agaaagaacc aaagcaaaca tcagcttttt
    19201 cccaggaaaa tcaagcttcg caaatccata agcaataaaa gaatttgaaa ggacattacc
    19261 gatcactact agcacagtga tgaataatgt atttcttgca tagcgccaaa aaggaaaggc
    19321 ggcaatcgta tccagatagt tttcccatac gattttttct gggaaaaagg tcggaggata
    19381 acgaacgatt tcttccattg gtttcaatga agtaaacacc atccaaagca atggaatgat
    19441 caaaatgatt cctattactg tcatgaggac ataacttagt atttttttgt ttgccgattc
    19501 acactggtct ttttgaaata atccatcatt tgccgtcacc tccagaataa tagacccact
    19561 taggtgcaag cttcaaattg acaaacgtca gaaccaacac gatcagaaac aggatcattg
    19621 acatagccat cgcataaccc atatcaaaag ctacgaacgc cttgttccac atatgcagat
    19681 tataaaaaag caaagaattt gcaggtccgc ctgttccatt tttcgtcatg acataagctt
    19741 cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa ataataggag
    19801 tgattaatgg caaagtaata cttttgaact ttccccaaac gcctgctcca tcgatttctg
    19861 ctgattcata taaagatcta ggaacatttt gtaatgtagc taagtaaagt agcattgctc
    19921 ctccagaact ccatattttc atcaaaatca acgcaggttt cgtccattgt ggatcaaaca
    19981 gccaagctgg tccagggata tggaaccagc ccagaaatgt gttgatcaat cctgcagatg
    20041 gagaaagcag ctgcatccat aagatgtaga ctgctactcc tgataagact gccggtaaat
    20101 agaaagctgt acggaataca ctgattcctt tgattttttg attgagtact gtggctaaaa
    20161 acacactgaa aatcgtcgtc agcggtacat tgaagatgac gaaataaacg gtgttgtata
    20221 acgatttcca aaataaatca tcgtgaacga acattctttt gaaatttgct agaccaatga
    20281 aattcatctt ggatgtcaaa ttataatcgg tgaaactacc aataactgaa aaaagcatag
    20341 gccctagcat aaataccaag aacccgataa taaaaggcga gataaataaa taggcccatc
    20401 ttgcttcttt tttctttcgg atagataaac gcttcaaatt ttcttgcctc ctctgtacct
    20461 agacagtcaa aaatggatta ttgattttgc ttgactaagt tttctactgc tttttgtgca
    20521 tctgccaatc cttcttttgg tgattttgtt cctaaaagaa tctggtcaat ctgttcattc
    20581 actaatgtcg tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt atttttaaag
    20641 ttttcgtccg ccatttgata gatcattttt cctttatccg ttaggttgtc attattcact
    20701 aaattctcat tggctgtctt gctcgccatg atatcaaaac ttttttcgcc gaatttttct
    20761 tgtacttctg gagaagaaag atatttgatg aattcataag aggcctcagg atcttttgct
    20821 ccgtatggta cttccaagac gaatccgcca ccccacgacc aatggcctga accttcttct
    20881 ttttctggga gctgcatcac gccaaaatca aaatcatccg gtgcattttc catcaaacta
    20941 gagtaataat tgatattttg ggcacgcatg gctactaatc cggaaataaa tggatcagat
    21001 acaccagacc caaattcggc ttctaattta ttgatcgtat ttttcccata ataatcttgc
    21061 cagtcttgga tccactccaa ggcttccact ttatttttag tatcaatctt gactttttca
    21121 tctttgtcaa accaactagt cccgttatct gcgtttagcg cccatacatc agcccctaaa
    21181 ttccataatg gataaaaacc gatgcgtgta aattcttttc cttctttttt atctagttta
    21241 cgtgctgttt cttctaattc atcccaagtt gtcggtagat cttgttcact gattccagct
    21301 tctttaaata tctttttatt ataaaaaatg acttgtgtat ctgtattgaa tgggatcgca
    21361 taaggatcac cttcatattc tactgtatcc caaagctgcg gataaaaatc cttagaagtg
    21421 tctttttcta catatttgga gagattagtt gcttgctgtg cttctgctct ttgggcaacg
    21481 gaatttatgt cctgtacaat aacatcgggt gcatttccag ctgttattgc agataaagat
    21541 ttagtccaga tgtcacccca tggctgatac ttataattta catggatctc gttttgtgat
    21601 tgattgaaat cctcaatgat ttcttcgata gcttcttttc gtgcaccgga tccccaaaag
    21661 gaccagaaat caatattttt tcttccatca cttgtttttt cagaagaatt tgctgcactt
    21721 gaactggcat cgcttgcatt acttgatccg catcctccta ataaaaacaa tgctgaactt
    21781 gccagcaacc ctgtaaacaa taattttttc atattttacc tccaaatttt aatgtaaccg
    21841 atgccggttt taatataacg atatcatttt gttctgaaaa taacaattta taaggtatga
    21901 ttctaaaaaa attattaatg ataccaatac tttatataat tcgcgagcgc tttctttgaa
    21961 ttttttaatc tttcgtagca gaatgagaat gtattaaaaa tagattagga ggaattttga
    22021 tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg aatccattaa
    22081 tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg cgagatgagt gtagctatgc
    22141 agtacctgtt ccaaggctgg agctgccgag gagaagaaaa atataaagat atgctgatgg
    22201 acattgcaac agaagaaatc ggccacgtgg aaatgttggc gacaatggtt gctcgtttat
    22261 tagagggtgc gcctgttgaa gatcaagaag cagcgatgca aaaagatcca acaatcggtg
    22321 ccattatctc tggaatgaat ccacagcacg cgatcgtagc tggcttagga cctcgtcctg
    22381 ctgacagcgt gggcaatcca tggagcggtg cctatatcat tgcgagtggc aacttgttag
    22441 cagactttag agctaattta aatgccgaat cacaaggaag acttcaagta gcacgtattt
    22501 acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt gcaagagata
    22561 gctatcatca aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa
    22621 tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa agacattgct tatgacttct
    22681 ataacttctc agagggagac caaagtagtg aaggttcttg ggctagtggc aaagcactgg
    22741 atggcgaagg cgaatatcaa tggttgaaag aacctaagat cgaaggcgct gcaccaaaat
    22801 tagaaccagt aacaccaaaa atgtatggga cacctccatt caagaaataa atatctaccc
    22861 gtagaaacac ccgttttttg ttccacacgc tttgtgagca aaactgacta aagtcattaa
    22921 tcataaccac ccttcaaaag ggtggttttc tctagagcta taagctcaaa tcaccggcca
    22981 gcgcctaaag acgctggctt tcatatttta tgttcaagcc atattgcctt tgccagtttt
    23041 gcccacctct taaaaggttt atgtactact ccttagccct taaaaggatc gtcttactct
    23101 cgtacactca gcttatctaa cgcactatca tgtttttctt gttcttgaat gtactttttg
    23161 atggtcgctt cattttgtcc tacttttgtc acatagtatc cttccaccca aaagtgtcga
    23221 tacccaaatt tatattttag atttgcacaa tctcttgatg atctcctgaa tattggctcg
    23281 atattgatta taaattattt ttcttctata ctttggtgtg aatacaatgt ggtatttaca
    23341 catctacttt gtacgggcta aactatttcc ctttattgct ccacccgcat agcaagtggt
    23401 tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc
    23461 gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt
    23521 acggcaatct attttatttt tttgattttt tataaatgga aaatgcttca agaagtccaa
    23581 gaaataaagc cgtataaaaa caagaaccta tgaaatcttt atttattaga tattcaacag
    23641 aaatcccgat aacactagcc aacaatatat ttatcagaag tgtaaaataa ggatgctttt
    23701 tatagaatag aactagtgtt gttttcatag actctccttt cttcatattt ccaattaatc
    23761 acaaattcac ggcattacta gaaaaattaa aggggcgcag aagtggaaaa atactcttcg
    23821 actttcccta caaaccaggc catcttgtgt ttaataacta taccaaatct aaggtacctc
    23881 taaatctaaa ttccatttgt ggcggtgacc tccaattttt aactcttcta tctcccatcc
    23941 agtatacata accttgatac ttcgaccact tatatatcga acacacaaat acctatccat
    24001 tgcaggagta ctacctaact tagttgctac ttgcatctaa ataacagctg aaaagatagg
    24061 acttactctt agtaaagtat ctgaactatt aatttttaaa ctatcatttt tatcctcaac
    24121 tacatgcaca tctgttgcaa cttcactttc tgtctcatcc agataaatga ttaaggaatc
    24181 gggaattgca atatcttcag ctgacgctgg aatagttgag ctaaattagt catcatatct
    24241 ttattataaa ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat
    24301 cattaataaa tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca
    24361 acatataccc tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc
    24421 atagacactt ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa
    24481 aaagtgactc aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt
    24541 acctaaaagc tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac
    24601 gggatgtaat caattagaga ataaactaat cataaaaggt acttttacat tcataattgt
    24661 cgctttgaga ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca
    24721 agtattggtg tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg
    24781 ctaatttctg tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc
    24841 gcatgatatg cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc
    24901 ttgatcccaa attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc
    24961 taaagagttt agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta
    25021 aagtattgtc gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga
    25081 cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat
    25141 tctttagttt aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt
    25201 tacttggcct ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt
    25261 acaatgctta aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta
    25321 agttttctat ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa
    25381 ttacttgtcc ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta
    25441 tagtggaagt aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag
    25501 agacgactag tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa
    25561 tccatacaat atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc
    25621 tctcccttca ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc
    25681 accactaaat ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac
    25741 aacgatatat tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac
    25801 tacagaactt ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa
    25861 agtatactat ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg
    25921 ttttgtttaa agtatatctt attggatata aatagaaata aattgtagcc agttcatttt
    25981 ttctttaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
    26041 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
    26101 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
    26161 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
    26221 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
    26281 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
    26341 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
    26401 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
    26461 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
    26521 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
    26581 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
    26641 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
    26701 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
    26761 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
    26821 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
    26881 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg
    26941 gaaaacacaa cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc
    27001 tgcacgggca ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat
    27061 ttccggagtg aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa
    27121 aacttctcca gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata
    27181 ataattctga aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc
    27241 tcccaatctt tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc
    27301 aatttttgta gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct
    27361 cctgccaact cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga
    27421 aattgttcct tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt
    27481 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca
    27541 caaaattttt cttaatactc gaaactaagc gactttttca caacctcttc cttaaacggt
    27601 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agcagctatt
    27661 tttatttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa
    27721 cggtgttcaa tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    27781 ttttgccgtt tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca
    27841 cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag
    27901 caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat
    27961 tattaaagaa ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta
    28021 agtagtgata gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc
    28081 aacgctttgg cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg
    28141 atattttgtt tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat
    28201 tcttcatcca ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg
    28261 atctgtgtct catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc
    28321 tgttttccag catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca
    28381 tggatcaccg ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt
    28441 cctgtatcag aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa
    28501 ataagatctt tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt
    28561 catttctcat agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa
    28621 aaaagactgt gccaaattat ctgacacagc cctcactata aaactgttct catatagcta
    28681 gatgttcatt gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct
    28741 ctaatacttc ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg
    28801 catccattgc ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc
    28861 cactctctac caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa
    28921 gaattactgt gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc
    28981 caaagatttt atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt
    29041 cgataaaatc ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca
    29101 tctgctgctc aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg
    29161 ggtaaaaata atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca
    29221 tttcaatcat attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc
    29281 caccgcctgc tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt
    29341 tattattgaa catggtggat tctccattcg ttatttattt taatacatca tttagagctt
    29401 cactcattgt cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat
    29461 tcatagctag cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc
    29521 ctagaatcaa gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta
    29581 atacttgtgc tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt
    29641 gtgctttggc ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac
    29701 tgtaaggcac agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca
    29761 taatgattcg ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc
    29821 caatcgccca aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc
    29881 gatctgttag tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc
    29941 cagttgcagc caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg
    30001 tgacggcagc tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt
    30061 cgattcccgc atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg
    30121 gaagaaattc agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg
    30181 atgcaaattc caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt
    30241 cctgatccat cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg
    30301 gtaaaatgat tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt
    30361 tttcaccatt attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca
    30421 tcactgtccc agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg
    30481 tcaatttttc tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa
    30541 tcaatgattt agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg
    30601 acttttcaac gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac
    30661 cgccctttcc aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc
    30721 ttttttacct accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc
    30781 atctgcttta tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg
    30841 gttcgatttc ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt
    30901 gctttggagt aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg
    30961 ctgatgccat cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa
    31021 ttataaagag agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc
    31081 atatgttgta ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga
    31141 tttgttcctt ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt
    31201 gcctcgactt ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc
    31261 attgtactgc tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa
    31321 gtaaggcgct ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat
    31381 ttttccaaaa aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata
    31441 aatagcaatc ttttctacat aatcttttat atgattgatt ttaagataca tatataccag
    31501 aaagttgacc ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc
    31561 ctccttttgc tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc
    31621 aagcaactag cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa
    31681 aatgtccgaa taattttgct aattttacta acgccaactt gttctttccg aattcatcat
    31741 tttctatctg taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt
    31801 ttccgtatgg ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca
    31861 tccctttttc ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc
    31921 gtggttcatc gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact
    31981 ctttgatttg ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga
    32041 tctttgcgat gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc
    32101 cgcttgtttc gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat
    32161 tgattacatt tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga
    32221 atctttgtag atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt
    32281 ttaatacgat tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag
    32341 cactgacaac tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat
    32401 ttgctaaaac agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat
    32461 gattaggaaa tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc
    32521 tattcaatac tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt
    32581 tccaaccatt tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta
    32641 aatcttgcgc ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt
    32701 ttattttttg ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg
    32761 aggtaaagaa ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg
    32821 cagcatcgca cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta
    32881 attcgaaatc gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca
    32941 gtgctggtcc gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat
    33001 cggttgattt cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca
    33061 tgtctgtcgt caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg
    33121 atccattttg cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat
    33181 tttttccagc ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa
    33241 tgtatctaca ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt
    33301 cgttaccaca tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg
    33361 gatacgcaaa acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga
    33421 tggctggtta aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt
    33481 catttctcga atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag
    33541 ccgcgcaagc agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag
    33601 tgcttgtcgc tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat
    33661 aagtaatatt tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt
    33721 gctaaccttt ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc
    33781 ttggatcgct gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg
    33841 cgatgaatgc ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta
    33901 agatttgggc acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt
    33961 ccggaagttt gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag
    34021 gctgtcggat catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat
    34081 acttttcttg cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag
    34141 gaatcagcca atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg
    34201 ctactcttcg tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg
    34261 attttttagg aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac
    34321 gccattcaat tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca
    34381 tacctctttt tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa
    34441 taagtgaagt aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt
    34501 tctattttta taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa
    34561 tgacaaaaca aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt
    34621 ttgcgggcgt attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt
    34681 ttgatttaac gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga
    34741 tcattgttcc gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag
    34801 cttcaaatct gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg
    34861 tcctattgtt tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt
    34921 tccatattat cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta
    34981 ctttgacaac ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac
    35041 attttacatg gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta
    35101 tcggctggtt tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa
    35161 gcgttttagg aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa
    35221 cagtttccag ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc
    35281 gaagtaaaaa agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc
    35341 gcttgttttt gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct
    35401 tgccgaattt ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc
    35461 ttcccggcgc agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat
    35521 atggtccgaa gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat
    35581 tattcctact cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata
    35641 tgatcggtat cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg
    35701 aagaactaca aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg
    35761 tggctacttc attagtagcg gtcattatca attacgtcca gcatcatact tcccataact
    35821 atgaagtgtc tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc
    35881 tcgtaagctt tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat
    35941 atacggcagg tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg
    36001 cttttttatg gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag
    36061 ttacttagat acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac
    36121 taaagctagc gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta
    36181 tcgtctggct aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga
    36241 tcaaacgatt tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga
    36301 attggctgtt cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg
    36361 gctttcacct gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg
    36421 tatcagtggt ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt
    36481 agcacaaaaa caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc
    36541 ttggctcatc aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg
    36601 cccggcactt aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa
    36661 agggcagctc ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat
    36721 gcttgacgga gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac
    36781 ccatgaaaac gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac
    36841 taagagtaca gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt
    36901 ccgtgtaggt acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga
    36961 tgatccaagc ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta
    37021 tatcggctat ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca
    37081 aaaggacatc tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta
    37141 aggatcccga gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa
    37201 tttctgaatc aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat
    37261 ctgagcttca atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga
    37321 cgtatcctca aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg
    37381 accaaacgac ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt
    37441 ttcaggtttg atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg
    37501 ctgtttcaaa aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa
    37561 aggattagca aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc
    37621 cgcgtctgat cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt
    37681 gatcttgata attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt
    37741 ttcgctaaga tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt
    37801 tccgtttcgt atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc
    37861 cacattctga caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt
    37921 tttatcaaag taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa
    37981 agcatcgtca tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt
    38041 tgattttcca gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc
    38101 atattgtttc atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc
    38161 aagcgtcaaa gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt
    38221 attatggaga gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg
    38281 atcacaaaag atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa
    38341 gatcttcgaa ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc
    38401 ttctggaatc agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt
    38461 tgtatggtaa aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta
    38521 tttgctttgt tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac
    38581 aatgcttcca tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc
    38641 atgtgagatt gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc
    38701 tgtcttccta tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga
    38761 tcgaatactg gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt
    38821 tgtactcatg gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat
    38881 tcgagttttt atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa
    38941 aaaccgagaa cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag
    39001 taccagccgt ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt
    39061 acatattgtt ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa
    39121 gcatctaatt gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat
    39181 tctttttgtg cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg
    39241 gcagcaggag ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct
    39301 gtttcagttg tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca
    39361 tttgaagttg ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa
    39421 tttttttcag caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta
    39481 gataatgaat cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct
    39541 acaaacattg cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa
    39601 ttcatattaa actccttttt ttctccgttt tttattcttt cgacgct
//
LOCUS       DAZUKD010000011        38333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000011 DAZUKD010000000
VERSION     DAZUKD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.011"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..1109
                     /locus_tag="ACG8N9_000477"
     CDS             39..1109
                     /locus_tag="ACG8N9_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896106.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1124..1792
                     /locus_tag="ACG8N9_000478"
     CDS             1124..1792
                     /locus_tag="ACG8N9_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896107.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1812..2390
                     /locus_tag="ACG8N9_000479"
     CDS             1812..2390
                     /locus_tag="ACG8N9_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4896108.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2791..3702
                     /locus_tag="ACG8N9_000480"
     CDS             2791..3702
                     /locus_tag="ACG8N9_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4896109.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4080..4997)
                     /gene="rnhC"
                     /locus_tag="ACG8N9_000481"
     CDS             complement(4080..4997)
                     /gene="rnhC"
                     /locus_tag="ACG8N9_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4896110.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5199..5636
                     /gene="zapA"
                     /locus_tag="ACG8N9_000482"
     CDS             5199..5636
                     /gene="zapA"
                     /locus_tag="ACG8N9_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4896111.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5694..6242
                     /locus_tag="ACG8N9_000483"
     CDS             5694..6242
                     /locus_tag="ACG8N9_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS4896112.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6357..8717
                     /locus_tag="ACG8N9_000484"
     CDS             6357..8717
                     /locus_tag="ACG8N9_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4896113.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8804..9118
                     /gene="trxA"
                     /locus_tag="ACG8N9_000485"
     CDS             8804..9118
                     /gene="trxA"
                     /locus_tag="ACG8N9_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4896114.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9300..11078
                     /gene="uvrC"
                     /locus_tag="ACG8N9_000486"
     CDS             9300..11078
                     /gene="uvrC"
                     /locus_tag="ACG8N9_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4896115.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11223..12122
                     /locus_tag="ACG8N9_000487"
     CDS             11223..12122
                     /locus_tag="ACG8N9_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896116.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12115..13377
                     /locus_tag="ACG8N9_000488"
     CDS             12115..13377
                     /locus_tag="ACG8N9_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896117.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13538..15435
                     /locus_tag="ACG8N9_000489"
                     /pseudo
     CDS             13538..15435
                     /locus_tag="ACG8N9_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
     gene            complement(15765..16484)
                     /locus_tag="ACG8N9_000490"
     CDS             complement(15765..16484)
                     /locus_tag="ACG8N9_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4896118.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16674..16721
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16821..17153
                     /locus_tag="ACG8N9_000491"
     CDS             16821..17153
                     /locus_tag="ACG8N9_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896119.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17140..19143
                     /locus_tag="ACG8N9_000492"
     CDS             17140..19143
                     /locus_tag="ACG8N9_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4896120.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19162..19632
                     /locus_tag="ACG8N9_000493"
     CDS             19162..19632
                     /locus_tag="ACG8N9_000493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4896121.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19653..20237
                     /locus_tag="ACG8N9_000494"
     CDS             19653..20237
                     /locus_tag="ACG8N9_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS4896122.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20252..21238
                     /locus_tag="ACG8N9_000495"
     CDS             20252..21238
                     /locus_tag="ACG8N9_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4896123.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21228..21539
                     /locus_tag="ACG8N9_000496"
     CDS             21228..21539
                     /locus_tag="ACG8N9_000496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4896124.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21561..23342
                     /locus_tag="ACG8N9_000497"
     CDS             21561..23342
                     /locus_tag="ACG8N9_000497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4896125.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23335..24711
                     /locus_tag="ACG8N9_000498"
     CDS             23335..24711
                     /locus_tag="ACG8N9_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4896126.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24718..25353
                     /locus_tag="ACG8N9_000499"
     CDS             24718..25353
                     /locus_tag="ACG8N9_000499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4896127.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25383..26738
                     /locus_tag="ACG8N9_000500"
     CDS             25383..26738
                     /locus_tag="ACG8N9_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4896128.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26930..28324)
                     /locus_tag="ACG8N9_000501"
     CDS             complement(26930..28324)
                     /locus_tag="ACG8N9_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4896129.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28416..29387
                     /locus_tag="ACG8N9_000502"
     CDS             28416..29387
                     /locus_tag="ACG8N9_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4896130.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29384..30166
                     /locus_tag="ACG8N9_000503"
     CDS             29384..30166
                     /locus_tag="ACG8N9_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4896131.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30335..30706)
                     /locus_tag="ACG8N9_000504"
     CDS             complement(30335..30706)
                     /locus_tag="ACG8N9_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4896132.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30840..31283
                     /locus_tag="ACG8N9_000505"
     CDS             30840..31283
                     /locus_tag="ACG8N9_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4896133.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31326..32135)
                     /gene="yidA"
                     /locus_tag="ACG8N9_000506"
     CDS             complement(31326..32135)
                     /gene="yidA"
                     /locus_tag="ACG8N9_000506"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4896134.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32154..33521)
                     /locus_tag="ACG8N9_000507"
     CDS             complement(32154..33521)
                     /locus_tag="ACG8N9_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4896135.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33505..34335)
                     /locus_tag="ACG8N9_000508"
     CDS             complement(33505..34335)
                     /locus_tag="ACG8N9_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4896136.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34498..34935
                     /locus_tag="ACG8N9_000509"
     CDS             34498..34935
                     /locus_tag="ACG8N9_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4896137.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            35011..35631
                     /gene="rpoE"
                     /locus_tag="ACG8N9_000510"
     CDS             35011..35631
                     /gene="rpoE"
                     /locus_tag="ACG8N9_000510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4896138.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35820..36326
                     /locus_tag="ACG8N9_000511"
     CDS             35820..36326
                     /locus_tag="ACG8N9_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4896139.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36343..37242
                     /locus_tag="ACG8N9_000512"
     CDS             36343..37242
                     /locus_tag="ACG8N9_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4896140.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37329..38213
                     /locus_tag="ACG8N9_000513"
     CDS             37329..38213
                     /locus_tag="ACG8N9_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4896141.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        12611 a         6548 c         8194 g        10980 t
ORIGIN      
        1 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
       61 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
      121 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
      181 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
      241 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
      301 aaacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
      361 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
      421 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag
      481 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
      541 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc
      601 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
      661 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
      721 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
      781 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
      841 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
      901 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
      961 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
     1021 gcttgttgat gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
     1081 aaatcgatcc gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt
     1141 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
     1201 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
     1261 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
     1321 caatgatcaa gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga
     1381 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
     1441 attattagta gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga
     1501 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
     1561 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
     1621 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
     1681 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
     1741 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
     1801 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
     1861 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa
     1921 aagttgggat cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata
     1981 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
     2041 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
     2101 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
     2161 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
     2221 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
     2281 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
     2341 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
     2401 tagggagaaa atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc
     2461 tttatatagg tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat
     2521 atttttattt attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa
     2581 ttgtttgtag aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa
     2641 ggagatttca ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta
     2701 cggttatatc ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct
     2761 tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt
     2821 gcaatcatta tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt
     2881 ggacaaacgg tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat
     2941 acagaacaag agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac
     3001 tcgctaacta ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa
     3061 acaaaaaatg cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa
     3121 gatggaacca aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg
     3181 attgtattag atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa
     3241 acagaagaaa aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat
     3301 gctggagcaa cggtttattt gacccatact acagatgaat tagtacagct tggagatata
     3361 tgcgactaca gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg
     3421 gaatatgcga atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca
     3481 ttagcacaga caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc
     3541 actgggaact atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt
     3601 tatatgaata atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt
     3661 gctgcaagtt tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa
     3721 caaaaatagc tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt
     3781 aaacaccgtt taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca caaggagcaa
     3841 tttcaaaaaa cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca
     3901 tgagaacacc gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag tattagagaa
     3961 acaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc gaggatcaga
     4021 tgattgaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat
     4081 tattttttta gtagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt
     4141 tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt
     4201 ccagctcctg acggaagggt gattccagct tcagctgatg ctttgtctaa ctcttccaaa
     4261 aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta
     4321 gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa
     4381 tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt
     4441 tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat
     4501 tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt
     4561 ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta
     4621 acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt
     4681 agcggaccga aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg
     4741 atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc
     4801 ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta
     4861 taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta
     4921 tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg
     4981 ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca
     5041 cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa
     5101 tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac
     5161 taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct
     5221 acaaagcggt gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata
     5281 tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac
     5341 aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga
     5401 agaaacaaga aagaatattg gagctggaag aagaaacagc agaattaaag aaaaaaatga
     5461 tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc
     5521 gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac
     5581 aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc
     5641 cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga
     5701 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
     5761 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
     5821 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc accccaacat
     5881 caaagttagc attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag
     5941 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
     6001 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
     6061 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
     6121 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg
     6181 agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt
     6241 aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc
     6301 tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga
     6361 ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg
     6421 tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa
     6481 cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg
     6541 ggatccctat acctaaaatt gaaaatatcc gtccccatat gaaacggatt gagatcggag
     6601 cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat
     6661 tgaatcgttt cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt
     6721 gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg
     6781 aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc
     6841 ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa
     6901 agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac
     6961 aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggtcagactt
     7021 tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg
     7081 ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc
     7141 gtcaagaaat catccacaat acttatgtga ttggtaaaat ggatctgatg aatgcaaaag
     7201 cacgatttgg taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg
     7261 tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca
     7321 caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga
     7381 tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg
     7441 caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac
     7501 agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt
     7561 tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc
     7621 cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt
     7681 atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata
     7741 cgattaatgc cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga
     7801 ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag
     7861 aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga
     7921 tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt
     7981 ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg
     8041 aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg
     8101 cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc
     8161 aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc
     8221 aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga
     8281 aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa
     8341 acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc
     8401 tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg
     8461 aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc
     8521 taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta
     8581 ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc
     8641 atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa
     8701 ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga
     8761 aatgctatac tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac
     8821 agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac
     8881 ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga
     8941 tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca
     9001 attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa
     9061 agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa
     9121 acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg
     9181 cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg
     9241 attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga
     9301 tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga
     9361 tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg
     9421 tccgctcata ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg
     9481 aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc
     9541 tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt
     9601 tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag
     9661 acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga
     9721 tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc
     9781 tttactatca tttgggacaa tgcctgtgtc cttattattt cgacgtagat ccgaaagtct
     9841 atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt
     9901 cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag
     9961 aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata
    10021 ctgatttact ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc
    10081 aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct
    10141 ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc
    10201 atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt
    10261 tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg
    10321 cgagtaagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg
    10381 aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac
    10441 gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg
    10501 tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca
    10561 acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga
    10621 ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag
    10681 atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg
    10741 taaaaaacga taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac
    10801 cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc
    10861 gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag
    10921 atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt
    10981 tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg
    11041 tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa
    11101 agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat
    11161 aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag
    11221 aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg
    11281 tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga
    11341 agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat
    11401 ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac
    11461 ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag
    11521 ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa
    11581 aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc
    11641 tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt
    11701 tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt
    11761 catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga
    11821 tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat
    11881 ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg
    11941 aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc
    12001 ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta
    12061 accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt
    12121 aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg
    12181 atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct
    12241 caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag
    12301 caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa
    12361 gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat
    12421 gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg
    12481 atacaacaac aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca
    12541 ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt
    12601 gattcaaaag gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt
    12661 tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa
    12721 gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca
    12781 caaaagcatt tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca
    12841 ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa
    12901 aatatattag acggtatctc attggataac ttgattggac cattcttgtg gtattctctt
    12961 ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg
    13021 gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc
    13081 ggatacatgc ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc
    13141 acttcgtata ttccattctt atcttcatat attatgccga ttcgcttagc aaatgagaca
    13201 gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg
    13261 atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta
    13321 tggagttcat tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac
    13381 aatcctaaaa tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac
    13441 ctattttatt tattttacta tattggatat atattcattg aaaatagtgt ataacaactt
    13501 tataatattg agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact
    13561 ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat
    13621 tttgcagctt ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc
    13681 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
    13741 ttctgaacaa atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga
    13801 atatgcatta acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt
    13861 gatcgatctt ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt
    13921 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg
    13981 aaaaaaagac tatgtaaaaa aatcattgaa caacttgttt tgatgaagga agaagaaaaa
    14041 tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt gcatatcgtt
    14101 ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat catggatttt
    14161 tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc gtattgcggc
    14221 tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta tttatttgaa
    14281 ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca tcaaacttat
    14341 cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag agagcaagaa
    14401 gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga acatcagtta
    14461 ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa aatcgactat
    14521 gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac ttgttttcaa
    14581 acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt gattgatagt
    14641 ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt tttgatcgaa
    14701 aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg tatacctgta
    14761 gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
    14821 ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc aaaagaagat
    14881 ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt attcggcaag
    14941 agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca cgtcgagaaa
    15001 agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttagcagaa
    15061 ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt aaaagtaaaa
    15121 aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg aggtttatcc
    15181 aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa ttcttatggt
    15241 ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc aaaaaaatcc
    15301 gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact caatcgtgat
    15361 acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct gttggactat
    15421 cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga gactgagaca
    15481 aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa ctcctcggtc
    15541 acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc cttgtgactc
    15601 agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag ccagtttctc
    15661 ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat ttttcttaat
    15721 actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa ttcattcaca
    15781 aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc cggatgccat
    15841 tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaac gagtccatcg
    15901 ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg atgatggtaa
    15961 gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga aacaatcttg
    16021 acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac tgaccacttt
    16081 ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa tagttggagt
    16141 ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat cagtgcctgt
    16201 tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc ttccatgtaa
    16261 agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg tccaatcagt
    16321 tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg agcatctaaa
    16381 actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa agtatctgtt
    16441 gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat tccccctaaa
    16501 gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt gcccctatac
    16561 ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa ttcatgacga
    16621 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    16681 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    16741 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    16801 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    16861 cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag atctatgcaa
    16921 cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa ctaaaacagt
    16981 tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa atgaaattga
    17041 aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag catcagagtc
    17101 aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    17161 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    17221 atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtggag
    17281 aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggtaat
    17341 cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    17401 aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc caattattcc
    17461 gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa gtgggaacaa
    17521 ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    17581 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    17641 agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga cgtttatatg
    17701 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    17761 gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    17821 gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat tgttcacgag
    17881 cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca agaatttgaa
    17941 tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga acggtttatc
    18001 catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    18061 ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt cgaaacacca
    18121 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttgtcgaa
    18181 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaaca
    18241 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttat
    18301 ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct accacgcggg
    18361 atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat ctggggattg
    18421 atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat ccgcttgcct
    18481 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    18541 ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat caggagaaaa
    18601 gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat tgggatcgtt
    18661 attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct tggcgctggg
    18721 ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac gccgtctaaa
    18781 gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta tatcggtgat
    18841 ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat tggagcggca
    18901 ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg gatccttttg
    18961 atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta tgtccatggc
    19021 gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg acgtgccttt
    19081 gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa aaacaataaa
    19141 taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg tggaatggta
    19201 tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc agctaaaggt
    19261 gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga aaaattcggg
    19321 caggctctga ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt cgttattgcc
    19381 tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact gaactttcta
    19441 ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca aggaaaagtc
    19501 gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    19561 tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt cctattagtg
    19621 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    19681 agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag cgaatgaaga
    19741 tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa aaacaaaaag
    19801 aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc cagcaagtag
    19861 aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg tttgcagatg
    19921 cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc aaaaatgcat
    19981 tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa tcagcagcct
    20041 atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg atggttttaa
    20101 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    20161 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    20221 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    20281 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    20341 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    20401 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    20461 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gttatacttt
    20521 ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg accatttgta
    20581 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    20641 aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    20701 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    20761 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    20821 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    20881 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    20941 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    21001 ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga aagatgatta
    21061 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    21121 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    21181 aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    21241 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    21301 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    21361 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    21421 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    21481 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    21541 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    21601 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    21661 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    21721 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    21781 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    21841 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    21901 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    21961 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    22021 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    22081 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    22141 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    22201 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    22261 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    22321 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    22381 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    22441 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    22501 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    22561 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    22621 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    22681 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    22741 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    22801 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    22861 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    22921 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    22981 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    23041 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    23101 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    23161 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    23221 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    23281 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    23341 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    23401 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    23461 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    23521 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    23581 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    23641 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    23701 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    23761 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    23821 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    23881 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    23941 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    24001 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    24061 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    24121 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    24181 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    24241 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    24301 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    24361 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    24421 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    24481 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    24541 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    24601 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    24661 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    24721 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    24781 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    24841 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    24901 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    24961 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    25021 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    25081 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    25141 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    25201 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    25261 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    25321 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    25381 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    25441 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    25501 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    25561 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    25621 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    25681 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    25741 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtga
    25801 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    25861 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    25921 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    25981 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    26041 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    26101 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    26161 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    26221 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    26281 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    26341 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    26401 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    26461 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    26521 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    26581 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    26641 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    26701 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    26761 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    26821 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    26881 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    26941 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    27001 aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat
    27061 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    27121 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    27181 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    27241 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    27301 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    27361 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    27421 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    27481 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    27541 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    27601 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    27661 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    27721 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    27781 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    27841 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    27901 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    27961 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    28021 cttcattggt agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc
    28081 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    28141 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    28201 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    28261 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    28321 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    28381 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    28441 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    28501 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    28561 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    28621 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    28681 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    28741 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    28801 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    28861 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    28921 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    28981 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    29041 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    29101 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    29161 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    29221 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    29281 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    29341 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    29401 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    29461 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    29521 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    29581 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    29641 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    29701 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    29761 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    29821 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    29881 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    29941 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    30001 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    30061 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    30121 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    30181 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    30241 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    30301 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    30361 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    30421 cagtaatctc aagaccagca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    30481 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    30541 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    30601 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    30661 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    30721 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    30781 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    30841 tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    30901 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    30961 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    31021 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    31081 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    31141 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    31201 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    31261 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    31321 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    31381 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    31441 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    31501 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    31561 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    31621 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    31681 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    31741 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    31801 tcatcgatcg aatggagatg actgccgaca cggcgtgcca ttacttcgat ctctaggaaa
    31861 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    31921 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    31981 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    32041 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    32101 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    32161 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    32221 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    32281 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    32341 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    32401 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    32461 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    32521 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    32581 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    32641 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    32701 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    32761 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    32821 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    32881 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    32941 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    33001 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    33061 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    33121 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    33181 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    33241 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    33301 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    33361 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    33421 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    33481 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    33541 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    33601 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    33661 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    33721 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    33781 ttgataaaat cgcttcatga cttgcccacg tttttcctga ttaccgttga cacttaagta
    33841 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    33901 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    33961 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    34021 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    34081 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catcttccag
    34141 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    34201 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    34261 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    34321 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    34381 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    34441 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    34501 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    34561 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    34621 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    34681 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    34741 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    34801 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagc agggaaggtt
    34861 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    34921 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    34981 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    35041 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    35101 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    35161 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    35221 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    35281 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    35341 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    35401 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    35461 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    35521 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    35581 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    35641 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
    35701 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
    35761 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
    35821 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
    35881 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
    35941 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
    36001 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
    36061 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
    36121 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
    36181 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
    36241 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
    36301 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
    36361 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
    36421 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
    36481 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
    36541 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
    36601 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
    36661 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
    36721 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
    36781 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
    36841 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
    36901 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
    36961 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
    37021 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
    37081 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
    37141 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
    37201 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
    37261 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
    37321 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
    37381 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
    37441 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
    37501 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
    37561 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
    37621 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
    37681 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
    37741 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
    37801 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
    37861 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
    37921 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
    37981 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
    38041 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
    38101 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
    38161 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
    38221 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
    38281 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAZUKD010000012        37834 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000012 DAZUKD010000000
VERSION     DAZUKD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.012"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..3337
                     /locus_tag="ACG8N9_000514"
     CDS             59..3337
                     /locus_tag="ACG8N9_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4896142.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3878..5785)
                     /locus_tag="ACG8N9_000515"
     CDS             complement(3878..5785)
                     /locus_tag="ACG8N9_000515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4896143.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5915..6790
                     /locus_tag="ACG8N9_000516"
     CDS             5915..6790
                     /locus_tag="ACG8N9_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4896144.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6870..7844
                     /locus_tag="ACG8N9_000517"
     CDS             6870..7844
                     /locus_tag="ACG8N9_000517"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4896145.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8024..9169
                     /locus_tag="ACG8N9_000518"
     CDS             8024..9169
                     /locus_tag="ACG8N9_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4896146.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9306..9650
                     /locus_tag="ACG8N9_000519"
     CDS             9306..9650
                     /locus_tag="ACG8N9_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4896147.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9921..11207
                     /gene="efmA"
                     /locus_tag="ACG8N9_000520"
     CDS             9921..11207
                     /gene="efmA"
                     /locus_tag="ACG8N9_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4896148.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11345..11944)
                     /locus_tag="ACG8N9_000521"
     CDS             complement(11345..11944)
                     /locus_tag="ACG8N9_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4896149.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12348..13469
                     /gene="mnmA"
                     /locus_tag="ACG8N9_000522"
     CDS             12348..13469
                     /gene="mnmA"
                     /locus_tag="ACG8N9_000522"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4896150.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13715..15136
                     /gene="arcD"
                     /locus_tag="ACG8N9_000523"
     CDS             13715..15136
                     /gene="arcD"
                     /locus_tag="ACG8N9_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4896151.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15150..16268
                     /locus_tag="ACG8N9_000524"
     CDS             15150..16268
                     /locus_tag="ACG8N9_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4896152.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16328..17620)
                     /locus_tag="ACG8N9_000525"
     CDS             complement(16328..17620)
                     /locus_tag="ACG8N9_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4896153.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17770..18330
                     /locus_tag="ACG8N9_000526"
     CDS             17770..18330
                     /locus_tag="ACG8N9_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4896154.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18459..18836
                     /locus_tag="ACG8N9_000527"
     CDS             18459..18836
                     /locus_tag="ACG8N9_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4896155.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18769..20841
                     /locus_tag="ACG8N9_000528"
     CDS             18769..20841
                     /locus_tag="ACG8N9_000528"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4896156.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21052..21999)
                     /locus_tag="ACG8N9_000529"
     CDS             complement(21052..21999)
                     /locus_tag="ACG8N9_000529"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4896157.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22245..22709
                     /locus_tag="ACG8N9_000530"
     CDS             22245..22709
                     /locus_tag="ACG8N9_000530"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4896158.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22771..23814)
                     /locus_tag="ACG8N9_000531"
     CDS             complement(22771..23814)
                     /locus_tag="ACG8N9_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4896159.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24191..25156)
                     /locus_tag="ACG8N9_000532"
     CDS             complement(24191..25156)
                     /locus_tag="ACG8N9_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4896160.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25390..26235
                     /locus_tag="ACG8N9_000533"
     CDS             25390..26235
                     /locus_tag="ACG8N9_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4896161.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26426..27613
                     /locus_tag="ACG8N9_000534"
     CDS             26426..27613
                     /locus_tag="ACG8N9_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4896162.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27619..27975
                     /locus_tag="ACG8N9_000535"
     CDS             27619..27975
                     /locus_tag="ACG8N9_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4896163.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27977..28315
                     /locus_tag="ACG8N9_000536"
     CDS             27977..28315
                     /locus_tag="ACG8N9_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4896164.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28391..28647
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28710..29744
                     /locus_tag="ACG8N9_000537"
     CDS             28710..29744
                     /locus_tag="ACG8N9_000537"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4896165.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29737..30423
                     /locus_tag="ACG8N9_000538"
     CDS             29737..30423
                     /locus_tag="ACG8N9_000538"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4896166.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30447..31295
                     /locus_tag="ACG8N9_000539"
     CDS             30447..31295
                     /locus_tag="ACG8N9_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4896167.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31492..32265
                     /gene="sufC"
                     /locus_tag="ACG8N9_000540"
     CDS             31492..32265
                     /gene="sufC"
                     /locus_tag="ACG8N9_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4896168.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32278..33564
                     /gene="sufD"
                     /locus_tag="ACG8N9_000541"
     CDS             32278..33564
                     /gene="sufD"
                     /locus_tag="ACG8N9_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4896169.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33561..34796
                     /locus_tag="ACG8N9_000542"
     CDS             33561..34796
                     /locus_tag="ACG8N9_000542"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4896170.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34783..35253
                     /gene="sufU"
                     /locus_tag="ACG8N9_000543"
     CDS             34783..35253
                     /gene="sufU"
                     /locus_tag="ACG8N9_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4896171.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35258..36649
                     /gene="sufB"
                     /locus_tag="ACG8N9_000544"
     CDS             35258..36649
                     /gene="sufB"
                     /locus_tag="ACG8N9_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4896172.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36747..>37834)
                     /locus_tag="ACG8N9_000545"
     CDS             complement(36747..>37834)
                     /locus_tag="ACG8N9_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4896173.1"
                     /translation="TYLGKDPKTGKDIRTKRRRFKSKAEAQTALNRLLVDFKEKQEFK
                     INVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAFGELRLDKISVSYCQKQV
                     NEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLPRKIEEEQPLKFYTKDQL
                     QLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDIDYFNKTITIGKTIAQDEF
                     NQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILYGYNTNSPKQFLFTTNTN
                     KLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFESGASIKEVQERLGHKDIK
                     TTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT        12298 a         6970 c         8058 g        10508 t
ORIGIN      
        1 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
       61 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      121 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      181 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      241 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      301 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      361 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      421 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      481 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      541 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      601 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      661 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      721 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      781 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      841 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      901 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
      961 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1021 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1081 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1141 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1201 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1261 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1321 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1381 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1441 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1501 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1561 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1621 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1681 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1741 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1801 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1861 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1921 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     1981 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2041 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2101 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2161 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2221 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2281 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2341 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2401 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2461 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2521 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2581 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2641 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2701 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2761 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2821 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2881 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     2941 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3001 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3061 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3121 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3181 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3241 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3301 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3361 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3421 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3481 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3541 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3601 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3661 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3721 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3781 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3841 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3901 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     3961 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4021 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4081 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4141 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4201 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4261 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4321 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4381 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4441 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4501 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4561 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4621 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4681 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4741 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4801 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4861 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4921 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     4981 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5041 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5101 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5161 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5221 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5281 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5341 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5401 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5461 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5521 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5581 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5641 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5701 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5761 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5821 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5881 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     5941 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6001 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6061 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6121 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6181 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6241 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6301 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6361 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6421 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6481 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6541 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6601 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6661 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6721 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6781 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6841 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6901 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     6961 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7021 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7081 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7141 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7201 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7261 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7321 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7381 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7441 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7501 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7561 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7621 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7681 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7741 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7801 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7861 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7921 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     7981 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8041 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8101 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8161 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8221 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8281 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8341 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8401 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8461 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8521 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8581 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8641 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8701 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8761 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8821 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8881 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     8941 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9001 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9061 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9121 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9181 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9241 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9301 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9361 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9421 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9481 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9541 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9601 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9661 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9721 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9781 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9841 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     9901 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     9961 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10021 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10081 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10141 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10201 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10261 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10321 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10381 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10441 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10501 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10561 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10621 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10681 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10741 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10801 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10861 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    10921 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    10981 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11041 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11101 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11161 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
    11221 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11281 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11341 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11401 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
    11461 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11521 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11581 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11641 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11701 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11761 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11821 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    11881 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
    11941 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12001 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
    12061 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12121 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12181 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12241 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12301 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12361 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12421 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12481 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12541 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12601 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12661 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12721 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12781 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12841 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    12901 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
    12961 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13021 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13081 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13141 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13201 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13261 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13321 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13381 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13441 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13501 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13561 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13621 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
    13681 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
    13741 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13801 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13861 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
    13921 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    13981 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14041 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
    14101 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14161 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14221 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14281 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14341 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14401 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14461 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14521 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14581 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14641 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14701 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14761 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14821 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    14881 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
    14941 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15001 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15061 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15121 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15181 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15241 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
    15301 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15361 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15421 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15481 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15541 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15601 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15661 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15721 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15781 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15841 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    15901 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    15961 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16021 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16081 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16141 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16201 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16261 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16321 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
    16381 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16441 tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa
    16501 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
    16561 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
    16621 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
    16681 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16741 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16801 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
    16861 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    16921 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    16981 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17041 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17101 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17161 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17221 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17281 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17341 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
    17401 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17461 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17521 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17581 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17641 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
    17701 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17761 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17821 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    17881 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    17941 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    18001 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18061 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    18121 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    18181 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    18241 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    18301 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    18361 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18421 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18481 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    18541 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18601 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18661 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    18721 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    18781 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18841 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    18901 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    18961 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19021 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19081 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19141 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19201 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19261 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19321 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19381 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19441 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19501 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19561 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19621 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19681 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19741 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19801 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19861 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    19921 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    19981 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20041 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    20101 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    20161 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20221 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    20281 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20341 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20401 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20461 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20521 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20581 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20641 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20701 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20761 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20821 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    20881 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    20941 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    21001 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    21061 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    21121 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    21181 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    21241 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    21301 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    21361 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    21421 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    21481 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    21541 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    21601 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    21661 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    21721 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    21781 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    21841 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    21901 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    21961 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    22021 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    22081 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    22141 tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag
    22201 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    22261 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    22321 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    22381 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    22441 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    22501 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    22561 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    22621 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    22681 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    22741 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    22801 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    22861 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    22921 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    22981 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    23041 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    23101 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    23161 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    23221 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    23281 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    23341 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    23401 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    23461 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    23521 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    23581 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    23641 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    23701 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    23761 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    23821 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    23881 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    23941 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    24001 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    24061 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    24121 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    24181 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    24241 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    24301 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    24361 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    24421 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    24481 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    24541 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    24601 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    24661 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    24721 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    24781 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    24841 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    24901 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    24961 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    25021 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    25081 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    25141 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    25201 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    25261 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    25321 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    25381 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    25441 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    25501 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    25561 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    25621 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    25681 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    25741 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    25801 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    25861 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    25921 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    25981 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    26041 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    26101 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    26161 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    26221 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    26281 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    26341 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    26401 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    26461 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    26521 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    26581 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    26641 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    26701 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    26761 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    26821 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    26881 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    26941 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    27001 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    27061 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    27121 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    27181 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    27241 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    27301 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    27361 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    27421 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    27481 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    27541 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    27601 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    27661 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    27721 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    27781 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    27841 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    27901 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    27961 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    28021 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    28081 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    28141 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    28201 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    28261 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    28321 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    28381 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    28441 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    28501 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    28561 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    28621 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    28681 aaaaattata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    28741 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    28801 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    28861 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    28921 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    28981 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    29041 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    29101 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    29161 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    29221 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    29281 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    29341 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    29401 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    29461 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    29521 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    29581 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    29641 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    29701 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    29761 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    29821 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    29881 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    29941 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    30001 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    30061 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    30121 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    30181 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    30241 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    30301 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    30361 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    30421 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    30481 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    30541 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    30601 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    30661 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    30721 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    30781 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    30841 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    30901 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    30961 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    31021 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    31081 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    31141 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    31201 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    31261 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    31321 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    31381 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    31441 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    31501 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    31561 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    31621 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    31681 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    31741 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    31801 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    31861 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    31921 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    31981 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    32041 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    32101 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    32161 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    32221 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    32281 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    32341 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    32401 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    32461 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    32521 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    32581 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    32641 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    32701 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    32761 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    32821 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    32881 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    32941 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    33001 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    33061 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    33121 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    33181 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    33241 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    33301 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    33361 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    33421 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    33481 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    33541 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    33601 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    33661 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    33721 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    33781 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    33841 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    33901 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    33961 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    34021 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    34081 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    34141 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    34201 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    34261 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    34321 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    34381 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    34441 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    34501 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    34561 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    34621 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    34681 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    34741 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    34801 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    34861 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    34921 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    34981 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    35041 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    35101 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    35161 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    35221 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    35281 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    35341 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    35401 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    35461 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    35521 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    35581 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    35641 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    35701 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    35761 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    35821 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    35881 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    35941 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    36001 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    36061 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    36121 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    36181 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    36241 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    36301 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    36361 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    36421 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    36481 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    36541 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    36601 cgaactcaat cgattgatca gttatgagat ggaagggtct gtaggttaat aactatttca
    36661 ttgctcgaaa cgttgatatt aaagcgtttc gagcgttttt ttatttgtat tttaatggtt
    36721 ggctacgttt ttggctacgt ttggaattat tcgcccataa attttgcaaa tcggtcacca
    36781 gttttcttaa cagattgtgg tgtgacatga gcatagatat tcatggtagt tttaatatct
    36841 ttatgaccta atcgttcctg tacttctttt atcgacgcac cactttcaaa caataagcta
    36901 cagtgggtat gcctaaatcc atgtggagta atttgaggct ccatcggtgt ctttttgtaa
    36961 atccagtcta accaatcgtt tactacttgg gggtagtaca acttgtttgt gtttgtagtg
    37021 aaaagaaact gttttggtga attcgtgtta tagccatata agatcattat tttcatttgt
    37081 tcttgttgcc aaatacgcag ttgtttaagt gtaaaatcat caagctgaat cgttcttgat
    37141 gaatttttag ttttaggtac ttgtagaact acttgattaa actcgtcttg agcgatagtt
    37201 ttaccaattg ttattgtttt attgaaatag tctatatctt cccattgaag cgccaatgct
    37261 tcgcttttac gcattcctgt ataagctaat aaacggaaaa aagtgtacag tttaactgaa
    37321 ttattttctt tagtattctg taaaaacagt tgtaattgat ctttcgtgta aaacttcaag
    37381 ggttgttctt cttcaatttt tcttggaagc aatgttttag acataggatt atccttacag
    37441 acttccattg ctactccaaa tttaaatatt tgagcagttg tttttctcaa gaaaccatat
    37501 tgttttaatg ttttgtgcca ttcattcacc tgtttttggc aatagctaac acttatttta
    37561 tccaatcgta attccccaaa agcaggtaat atatggtttt tgcaaaaacg ttgagcaacg
    37621 gcaacagaag acggttttac tcttgttctg tagttatcaa tccataattc atacaactct
    37681 tgaaaagttg taacattgat tttaaactct tgtttttctt taaagtctac aagtagtcta
    37741 ttaagtgctg tttgcgcctc agcttttgat ttgaatcttc tacgctttgt acgtatatct
    37801 tttccagtct tgggatcttt ccctaaataa gttt
//
LOCUS       DAZUKD010000013        37797 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000013 DAZUKD010000000
VERSION     DAZUKD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.013"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8N9_000546"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8N9_000546"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4896174.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8N9_000547"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8N9_000547"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4896175.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8N9_000548"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8N9_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4896176.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8N9_000549"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8N9_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4896177.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8N9_000550"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8N9_000550"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4896178.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8N9_000551"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8N9_000551"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4896179.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACG8N9_000552"
     CDS             complement(8557..9027)
                     /locus_tag="ACG8N9_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4896180.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACG8N9_000553"
     CDS             complement(9073..9498)
                     /locus_tag="ACG8N9_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4896181.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACG8N9_000554"
     CDS             complement(9565..9834)
                     /locus_tag="ACG8N9_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4896182.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACG8N9_000555"
     CDS             complement(9845..11440)
                     /locus_tag="ACG8N9_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4896183.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8N9_000556"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8N9_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4896184.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVSDQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8N9_000557"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8N9_000557"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4896185.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACG8N9_000558"
     CDS             15906..16880
                     /locus_tag="ACG8N9_000558"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4896186.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACG8N9_000559"
     CDS             17050..18450
                     /locus_tag="ACG8N9_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4896187.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACG8N9_000560"
     CDS             complement(18528..19250)
                     /locus_tag="ACG8N9_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4896188.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACG8N9_000561"
     CDS             complement(19329..19835)
                     /locus_tag="ACG8N9_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4896189.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACG8N9_000562"
     CDS             19958..21757
                     /locus_tag="ACG8N9_000562"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4896190.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23399)
                     /locus_tag="ACG8N9_000563"
     CDS             complement(21825..23399)
                     /locus_tag="ACG8N9_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4896191.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="ACG8N9_000564"
     CDS             complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="ACG8N9_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4896192.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="ACG8N9_000565"
     CDS             complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="ACG8N9_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4896193.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25176..25814)
                     /locus_tag="ACG8N9_000566"
     CDS             complement(25176..25814)
                     /locus_tag="ACG8N9_000566"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4896194.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25815..26465)
                     /gene="rpe"
                     /locus_tag="ACG8N9_000567"
     CDS             complement(25815..26465)
                     /gene="rpe"
                     /locus_tag="ACG8N9_000567"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4896195.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="ACG8N9_000568"
     CDS             complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="ACG8N9_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4896196.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27542..29611)
                     /gene="pknB"
                     /locus_tag="ACG8N9_000569"
     CDS             complement(27542..29611)
                     /gene="pknB"
                     /locus_tag="ACG8N9_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4896197.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29608..30348)
                     /locus_tag="ACG8N9_000570"
     CDS             complement(29608..30348)
                     /locus_tag="ACG8N9_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4896198.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30361..31719)
                     /gene="rsmB"
                     /locus_tag="ACG8N9_000571"
     CDS             complement(30361..31719)
                     /gene="rsmB"
                     /locus_tag="ACG8N9_000571"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4896199.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31719..32657)
                     /gene="fmt"
                     /locus_tag="ACG8N9_000572"
     CDS             complement(31719..32657)
                     /gene="fmt"
                     /locus_tag="ACG8N9_000572"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4896200.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32650..33141)
                     /gene="def"
                     /locus_tag="ACG8N9_000573"
     CDS             complement(32650..33141)
                     /gene="def"
                     /locus_tag="ACG8N9_000573"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4896201.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33156..35564)
                     /gene="priA"
                     /locus_tag="ACG8N9_000574"
     CDS             complement(33156..35564)
                     /gene="priA"
                     /locus_tag="ACG8N9_000574"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4896202.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35817..36122)
                     /gene="rpoZ"
                     /locus_tag="ACG8N9_000575"
     CDS             complement(35817..36122)
                     /gene="rpoZ"
                     /locus_tag="ACG8N9_000575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4896203.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36127..36741)
                     /gene="gmk"
                     /locus_tag="ACG8N9_000576"
     CDS             complement(36127..36741)
                     /gene="gmk"
                     /locus_tag="ACG8N9_000576"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4896204.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37228..37512)
                     /locus_tag="ACG8N9_000577"
     CDS             complement(37228..37512)
                     /locus_tag="ACG8N9_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896205.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37530..>37797)
                     /locus_tag="ACG8N9_000578"
     CDS             complement(37530..>37797)
                     /locus_tag="ACG8N9_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HFS4896206.1"
                     /translation="RLLTELAILLERGDIHEELDRLAIHVQKLDELIEAQCPVGRELD
                     FLIQEMNREINTIGSKSNAIEIKNQVVQSKTILEKIREQVQNIE"
BASE COUNT        10212 a         8229 c         6888 g        12468 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta ccataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgacacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    22501 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22561 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22621 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22681 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22741 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22801 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22861 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22921 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22981 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    23041 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    23101 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    23161 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    23221 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    23281 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    23341 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    23401 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    23461 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    23521 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23581 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23641 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
    23701 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
    23761 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
    23821 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
    23881 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
    23941 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
    24001 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
    24061 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
    24121 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
    24181 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
    24241 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
    24301 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
    24361 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
    24421 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
    24481 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
    24541 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
    24601 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
    24661 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
    24721 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
    24781 gctcattttc ggcataagtt gaaaaaagat tatggacaga atctactcca tttcgtaccc
    24841 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
    24901 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
    24961 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
    25021 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
    25081 caaaaggtaa gttgctcctc ttaagctttt ttttagcaaa taatagattc tgtcattttt
    25141 agcctgaaag acaaattttt ttatttgttt atggatcaat ctttgctttg aataactgca
    25201 atcataccat ctgaaaaaga aaattctgcg gtatctgaaa gaaattcgtt acttgcataa
    25261 gatgtcggat attctacttc tttattttcc aatacatact tgctgccttt taatgtaaga
    25321 ttcgagacgg gagtcaagca gcaataagct aaatacttca tatctggtat ctgtcttact
    25381 atatgctctc caggcaaata ataagacaaa taattttgac aatctctgat aataagctga
    25441 tgggcaaagg gtttgaagcg aggttccata cctaaccaga gattggctaa aagatgatcc
    25501 aatcttccgc ccgtagctcc aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt
    25561 tttaagatac ctaactgtgt atctgtatcg tctttttctg caggggaacg ggtatactct
    25621 ttggcttttg caaatacttc cttctgctct tcgttcgaca atgaatcaaa atcacccact
    25681 gccagatcta ctgggtagcc tgcttgaagc aggtacagcg caccacggtc tatcccgaca
    25741 taggcatcgt atttggacag cagagactgc ggccagtttt gcggattacc tccagcagct
    25801 aataaaattt tcatttagtc taatgcctct tttaatcgtt cgatttgtcc gactggattt
    25861 tcggctccat agatatatga tcctgcaacg aagacatccg cccctgcatc tttacagatt
    25921 tttgctgttt ctggaacgat tccgccatct acttcgattg cataagtcca attgttctct
    25981 ctacgtagat cagcgagttc cgtaattttt tctactgttt cttcaataaa gctttgtccg
    26041 ccgaacccag gattgacagt catgactagt acttgatcag ccaaaggcaa aacatgtttg
    26101 atcatggaga ctggtgttcc tggattgata actactccag ctttaactcc taagcttttt
    26161 gtcatttgaa gtgcccgatg aatatgagga gttgcttcga cgtggacagt aatgatatct
    26221 gctccagctt ttgcaaatgc tggaatgtag ttttctggat caacgatcat cagatggacg
    26281 tctaacggta atttagtaac aggacggatt gcctgaacaa tatttggccc taatgtgata
    26341 tttgggacaa atcggccatc cattacatct acgtggatgt aatctgcgcc tcctttttcc
    26401 actaactcaa tgtctctttg taaattggca aaatctgcac tcaaaatgga tggtgctatc
    26461 ttcattcttg tttcctccta ttttcgttta ccttcttttt ttgcatacat tggttttctt
    26521 ttttcgattt cttgtaagaa ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct
    26581 gtttctactc tatgttttac ttcgcagcca ggttctttag catgcatgca ctctctaaat
    26641 ttgcaatgga cagaagcttc tacaaattca ggaaattgtc tcggcagttc tcctgcttca
    26701 attgtcaaaa agtcaatcga gctaaatcct ggtgtatctg ctactaaccc gccatagata
    26761 ggaatcagtt ccacatgacg tgttgtatgc cgtcctcttc caagtgcatc agaaatctct
    26821 cctgttgcta aattcagttc cggtgatatt tgattcagta gcgtcgattt tcctgctcca
    26881 gactgaccca taaagaccga tagccgttct gggaagtaac ggatcagttc tgtcgtatca
    26941 cccgtatgat tgggtacaat caccggatag cctattttag tataggtttg cttgatctct
    27001 tcgatttttt ctgggtcgtc tgacaagtcg gtctttgtca aaaaaataat cggttcgatc
    27061 gactcgtatt ccaaggtaac tagaaagcgg tcaagtagat tataagaaaa tgcaggctct
    27121 acgatacttg tgacaatgat tccaagatca acatttgtga ctggtgggcg aactaattga
    27181 ttttttctcg gcaagatttc cagaagatag ccgtctgttt gattttcact ctcaaagatc
    27241 acctgatctc cgacaagcgg cgtgattttc cgattccgga aatttcctct tcctcttgtt
    27301 tgataggtct ctccgtttgc gtatacatag taaaaaccgc tgattgcttt tctgatttgt
    27361 cctttgataa gtgccacctc cgtttttgtt tattttacca tacgaagagg aaagtaggtg
    27421 ttattccatg caaacttctc aaagtatatg gcttgaaaat tgatcaaaag agatagaaac
    27481 acaaaaaaga gaatgcaaca aatcaatgtc gcattccctt ctgatatatt ttattcagca
    27541 cttattttga actttctgag gtgctagctg atgaacttgt ggagctggaa gattcttctt
    27601 ttgaagagtt gcttgtgcta ctggagctac ttgagctgct agaactgtct gaagagtctt
    27661 tttcagaact agaagacgct ggatctggtc ctttagagta aattacgttc acaaccattc
    27721 ctttggtaat tgtcgttcct gcccctggta ctgtacgaat cactttatct ttatccaccg
    27781 tgtttgaata ttcggaagtc tcatgcccga tatattctgc accgacacta gctaaataac
    27841 tttgtgcttc gctttttgta tagccggaga tatcattcaa tttaggctct gttccttggc
    27901 ttacgacaaa attgatgtaa gtttcttctg ctacaacttc acccgaggct ggatcctgac
    27961 tgatgatcga acctgatggt acagtgtcgc tatagtcata agtagcacta atattattga
    28021 tattgaatcc ttgtgtaaca agccaattac gaacagtttc gtagttttct cctacatgac
    28081 tactcaaagt cactgtttct ggtcctttgc tgactgtgaa agtaatcgtg tctgttttag
    28141 gatcgacttc tgtatcttct gatggagatt gcgaaatgat cattccgctt tctacctctg
    28201 aactgaattc ctcttctttt tgaatcaagt cagcagaaaa ccctaattct ttcaaacttg
    28261 cttcggcatc ttcataactt ttaccactgt aatcatctaa tttgattttt ttcgtgccgc
    28321 tactaatata cagagtgact tcgcgttttt gcttgactgt cgttcccgct gccgggtctg
    28381 tcttcactac attaccttct tcgattttgt catctgcaat ttcttctgtt tttgttgcta
    28441 cttctaattt cgcttttgcg agtgtttctc tagcagaagc ttcagaaaga tcagaaacat
    28501 caggaacaac aacttctcct cgcccgccag aagcaaagaa tacagctgtt ccgattccta
    28561 aaacagcaag aatagctaat aggatcagcc accatttttt ctgtcctttc tttttaggat
    28621 caggcagaac ttcttcctcc tctggtaagg tttcgttttc tggcttgtct tttggcagag
    28681 gcatagattt gaatgaatca ggcattggtg catcttccgt aaggggtgtg ataacttttg
    28741 tttcattatc catcacatga ggctgccatt tttcttcgtt tgcacgagct ggtgataata
    28801 ctgtagaaag atcatcagac atttcctcgg ctgacttata acggtctgca ggttctttag
    28861 ccgttgcccg taaaatcacg ttttccagtg cttgtggcac atttggatca agtgctttta
    28921 tagatggaag atcatcttga aaatgtttta aagcaattgt tacagcagat tcaccatcaa
    28981 acggtacgct acccgtcaac atctcataaa ggatgatccc taaagcataa atatctgact
    29041 gcttcgttgc catacttcct ctggcctgct caggggacag ataatgaaca gatcctagca
    29101 tcgtattcgt ctgcgtgatc gatgtttcag ataaagcgat agcaatcccg aaatccgtga
    29161 tttttacaac accttcatta tcgatcagta cgttttgggg tttcaaatct cggtggatga
    29221 tttgatggtt atgagctaaa gaaatagcag aaaggatctg ctgcataata tttactgctg
    29281 tttcataagg aacaggataa tgtgtttgga tatatcgttt caagtccatt cctttgacgt
    29341 attccatcac cagatactgc atgttgtctt cttcaccaac atcgtaaaca ctaacaatgt
    29401 ttggatgaac gagttctgtt gctgctaaag cttctctctg aaatcgtcga atcgcagtct
    29461 ggtcattttg aaagtcaaat cgtaagactt taactgctac atcacgatcc aagattaaat
    29521 cgtgcgccaa aaatacattt gccataccac cgctaccgat atttccagtg atttgataac
    29581 ggccatttaa ttttttgccc aactcgatca tacactgtcc tccttatatt cgataagaag
    29641 aacggtgata ttatccgctc cgcctgcttc attggcacga tcaatcagct cagttacttt
    29701 atgagacaat gggccttctt gattgatgat cgttcctatt tcttcttcag acagcatgtt
    29761 cgtcaagcca tctgaacata acagcagtcg atcttttagt tgccaaagat gagtagaaac
    29821 atctacttcg actgttccag gcatgccgac agaacgtgtt aaaatatttt tccttggatg
    29881 gttcactgcc atttcttcgc tgatttctcc tgatttgact agttcattga ctaaagaatg
    29941 atcctccgtc aattgcatga ttttatcttc acgaatcaaa taagctctgc tatccccaac
    30001 attggcaatc gtgaaccagt catctgacag agcggccgct acgattgttg ttcccatacc
    30061 gttatattcc ggctgttctt gtccacgctg ataaatcagc atattttctt cttgaatcga
    30121 ttgaatgaac cattgggcga ttttttcatc gtcaacagct tcttgttttt cccaagcttc
    30181 gcctagattc gttaccgcca tctgactggc aatgtctccg gcacgatgac cacccatccc
    30241 gtctgctaat acagcaagct tgagacctga acggctggta aatacactgg catagtcttg
    30301 atttgtgtta cgtcgtcttc ctacatctga ttgatattca atctgcattt tttcacctca
    30361 ttactttttg cgcaaacaac aaatgaaaaa tccatctgtg taaaactgat gcggatacaa
    30421 agtcaacatt ttctcttcga ttgatgattg tagcatagga ttggtcaaaa catcaatcct
    30481 ctcaaaatca gggtgtcttt ccaagaaagc agaaacaacg tcttggtttt cttcttttaa
    30541 gatcgtacaa gtgctataag tcagaatacc ggaagacttt aaagttggtg cgcagctttc
    30601 taaaatttcc aattggattt ttggtaaatt cgctaattca tccggctgtt ttttataacg
    30661 aatatctggt tttcgccgaa gcagcccaag acctgaacaa ggcgcgtcta ctaaaatccg
    30721 atcaaactgc tctgcttcga actgtttact gacttctcta gcatccatct tttgagcgta
    30781 gacagcagaa gagacaccta gacgtgctgc attatcttca atgagtttga ctttgtgttc
    30841 gtgtacatct aaagcgacca cttctccacc gcatgacgga tctaaaaaag aagcaatatg
    30901 cgtcgtctta cctccgggtg ctgcacaggc atctaagaca tgatgttcag gctcgatttg
    30961 catagacggc gctacaagca tcgaactttc atcttggatc gtcatgacgc catcgtggaa
    31021 caatgagcta gaagctaaat gccctttttt cgctactata ccgtatggac tgacttggct
    31081 tttttcagct gtgatctctt cttcttgcaa aacagtgatg gcctgatctc tggaaagtcg
    31141 gcgtgtatct actcgggcac tagcatggct aggctgatat agcgataaac cgagcttttc
    31201 agtttcttcg tatccgattt gatccaccag tcgtttcgtc atccatagag gcaggctgat
    31261 tttgacagaa agtctttcga taggatcagc aatgccttgc agatctggtg ctccttgacg
    31321 ctgataattc ctcaagacac catttacaaa tttccctgtc ccaggatttc cttttacttt
    31381 tgcgatttcg accgcctcgt ttaaaatagc atgtgcaggg actttatcta aaaattctaa
    31441 ttggtataca gaaagataaa gaagcatctt gacccagtca tccacttttt ttgctttagc
    31501 gatcaatggt gcaacgtaaa attccaatag tcgctgatgg ctaatcgtac catagatcaa
    31561 ttctgtaaat aaacccacat ctttttgatt tagctcgctt tgtttgatca tctcattcaa
    31621 caaaagattc gagtaggcgc cacccttgtt cactcgctct aatgtcagta atgccatata
    31681 tcgtactgat tttttcattt ttttagggat ttttttcatt atttcacctg ctgaccaatc
    31741 tgaacttgct gaccgcttcc gttaaggaaa tcatgtacgg cctgttttcc tttaccagct
    31801 ggctgtaatt cattgatggc taagacggtc tgtttaccgc aagcaatcca tagatttttt
    31861 ttgtctttcc ggatgatcgt accaggtgct ttgtctgtag tttctgccaa tacttcaacc
    31921 gataataatt tccagcgtat gccctcatag gtcgtgaatg caattggcca aggacgcatt
    31981 ccgcgtactt ggttgtcaat ttcttctgca gttttatgcc aatcgattgc ttcttgttcc
    32041 cgtgtgatgt ttggcgaaaa agttgcttct gactcgtctt gaggtactgg tttttgtcca
    32101 tccaaaatat tgggcaatgt ttctagcaat agctttcttc ctaaaaggct aagcttttca
    32161 aacatcgtcc ctacatcatc ttgtttagtg attggtatgc tttcttgcgc atagattccg
    32221 cctgcatcca tttttttgat catttccatg atcgttacac cagtttcttt ctccccattc
    32281 atgatggcat aatgaacagg cgcacctccc cgatatttag gaagtaaaga agcatggaca
    32341 ttgattgccc ctaattttgg tacttgcaat aacttttcag gcaagaattg gccaaatgcc
    32401 gcagtgacga tcacatcagg caccaattca atgatttctt ccatttctgg cgaaccggag
    32461 attttctctg gctgcagcac acggataccg tgcttcagag ccgctttttt taccggtgtc
    32521 ggcgtaatta ctcgtttccg cccaactgga cggtcgggct gtgttaccac tgcctggatc
    32581 tcgtaacctg attctatcag cccttcaaga atcggcacag aaaaagcagg tgtgcccata
    32641 aatactagct tagtcatcaa gatgttcctc catatattct tcaagttctt cttccgggat
    32701 ctgctgtatc attttctcaa tgaacaagat accgtctaag tgatcgattt catgctggaa
    32761 ggcacgggct aaatagccaa aagccgtcac ttctatctct tctccgtctc gatcataata
    32821 acgcacggtc acttcatctg ctcgcttgac cgttccatag acatgcggaa tactgagaca
    32881 accttctacg tcaaggcttt caccttttgc ttcgatgatt tctgggttaa tcagttcaaa
    32941 tttctcgcct tcatctactt cgatcacagc aattctcttg ttttgtccta cttgaggagc
    33001 agcgatccct attccgtcat tcgcaatcat tgtctcatat aaattgtcta ataaagcgat
    33061 tgtctcgtcc gtgattacat cgattggctg agcagttctt tttaatttat cattagggtg
    33121 gatcaatata ggatagcgca taatttggcc ctttcttaaa taaagtttaa tggttcgttg
    33181 tcaattgcca caaaaagacc ttgtcgctgt tctttctggc ttccattcaa aatttcatcc
    33241 aaaacatgag gaagattcgg ttcatgttta tatttgatga ttagttgata ataatatcgg
    33301 tttttcactc tcaaaatcat gctgggcgtt ggccctaata gcaagctttg agggcttaac
    33361 acttctttta gctgattggc aatctgaaag attttctttg ccgctacttg ttcttcagga
    33421 tggctggcgg ttattttcac agtaaagtaa taaggaggat agcctccacg atgccgtaca
    33481 tgcatttcct gctgataaaa ctgctcataa ttttgttttt tagccaattc tatggcgtaa
    33541 tgttgaggat tgaatgtttg gatcacgact tcgcctgctt tttccgctcg ccctgctcgt
    33601 cctgaaactt gggtaagcaa ttggaatgta tgctcactag aacgaaaatc cggtaaattc
    33661 agggaggtat cagcattcaa caccccaact aatgtcactt ctggaaagtc caaaccttta
    33721 gcaatcattt gtgtccctaa taatatatct gcttcttttg caccgaattt ttgtaaaatc
    33781 tgttcgtgtg ccccttttct tcgagtggta tctacatcca tacgcaaaat cctagcttgc
    33841 ggataaagtt ctcggagttc ctcttctact ttttgagtac ctgttccata gtagcggatt
    33901 ttattgccgc cacaattagg acagcgattg ggaataggtt cttcatgtcc gcaataatgg
    33961 cagcgcattg aacgtgtatc catatgcagt gtaagagaga tatcgcaatt aggacatgga
    34021 agaacaaatc cacagtccct acacatcaca aaagacgaat atcctcgacg attgagcagt
    34081 aggacagttt gttctttttt gtctaatcga ttttgtattt tttcctgcaa attagcagaa
    34141 aaagtagagg tccgatgatt ctgaaactct tcacgcatat ctacgatctc aatagctgga
    34201 agctgtgcag ctgctttggc tcggtggttt aactgcaata actgataaac gcctttttga
    34261 gctcgtgctc ttgattctaa agagggcgtc gcgcttccta gaactattgg gcaatgatga
    34321 tatttactgc gccaaattgc tagatctctc gcatgataac gcggtgtctc atcttgtttg
    34381 taacttgatt catgttcttc atcgataatg atcagcccga tatttttcag cggggcaaaa
    34441 atagcagatc gtgcgcctac tactacttgc gcttcttccc gctcaatctt gcgccattca
    34501 tcatattttt caccttggga aagtccgcta tgtaagaccg caactgattc tccgaagcgt
    34561 cctttgaatc gctcgaccat ttgcggagtc aatgcgattt caggcactaa cataatagcc
    34621 gttttatttt ctgacaaaac atcagcgatt gcctgaagat atacttctgt tttcccgctg
    34681 ccagtgattc cctccagcaa aaagacctga tctttttgct gctgacctgc tgtgatgatt
    34741 tgttcgacag catttttttg ttctgcattc agttccaatg ccgtcgtttg gtcaaatact
    34801 cggtctttat aagggtcgcg ataacgttct gattctataa actccagcca gcccttttct
    34861 tttccttgat tcaagacagc agtactgatt cctttatttt tgaaataagc aattggttct
    34921 tcttggctca gttcagataa acacaaaaga agttcttttt gtttcttggc attttgccgt
    34981 aattttgccc attcttcttc gatttgcttt tcagttaaca aagaacggac caaacgaatc
    35041 gtttttactt tatttcgtgt atggacttca tatttgatat ctactaattg ctcttggcgc
    35101 cagcgaatca attgagaaag gctgccttct tcttctgctt ccttccagga aatcacttct
    35161 cgctgatcaa acaatacctg aacttctggt gtgtctgcca aaggatagat caatttatca
    35221 tattctgctt tcatcacact cggaagcatc gtttgtagac aagtgatttt gaatgcgtaa
    35281 gtaatttttt tcatataatc agctaatgaa agtaattctt cattgaccac cggagcaagg
    35341 tctagcaaac gaataactgc cttcaaagat ggattagctg attcttctga tttttggata
    35401 gccataacaa atccttggac atgacgattg ccattaccaa aagggacttc tactcgcatc
    35461 ccgacttgca aagctgtttc catttcagag ggaaccaaat aagtgaatgg ctgatcggtc
    35521 tgcatagtag gtacgtccac aatgacttca gctgttaatg tcatggttcg tttcctcctt
    35581 tttttgtctt ttcttatcaa gcgacaaaaa gttccttgcc tattttacta gagatcgatg
    35641 gatttgtctt atcttctgcc aaatcctaac aaaaaattaa cagcagataa gaaaaaaaag
    35701 aactgtgtca aaaagttcag acacagcctt ttctaccaga caataagtgg cgaatgtttg
    35761 aaaaggaaac agctgggttt gcttttcttt ttagatgttc gctctatttt tctttattac
    35821 attttttgct cttcacggat acgtgattcc aattcgtgtt gttcacgctc tttttgtgct
    35881 tgacgttcct cttcttccat cttcaaacgt tcacgtttta attcaggatg tggatcattg
    35941 actacatttc ctgcatcgat ttcttctaaa gcttgaccga cgctttttac tgaatcaaaa
    36001 gaatctagag taggctgtgc acttgcatcc aattcatgcg cgcgtttact tgctaaaatc
    36061 actagagaat attttgaatt tacacgatct aatagtgagt caattgaagg ttttaacatc
    36121 ataagactac atctccttta acattttaat atatttaccg attacgcgat ctactcgaaa
    36181 atgttcactg gcaataatgt ttttgattcg ttcaactgct agcggcactt catcatttac
    36241 aacagcatag tcgtaaagag ccatcatttc gatttcttct tttgcaactt tcattcgttt
    36301 ttcgattact tcatctgcat ctgttcctcg gcctacgatc cgtgatttta attccgctaa
    36361 atctggcgga gtcaaaaaga tgaatacacc atcgggcact ttttctttga cttgttttgc
    36421 tccttggact tcaatctcca agaaaacatc ttttccttca tccaatgtct tgttgacata
    36481 tgacaaagga gttccataat aattcccgac atattctgcg tattccagca tctctccagc
    36541 ttcgatcatt gattcaaact cttctttgct tcggaagaaa taatcaacgc cttcgacctc
    36601 cccttcacgc attttacggg tagtcattga gatcgaatac tgaaaatcat tatcctcact
    36661 ctcaaaaatt gcttttcgta ctgtgccttt tcctacaccg gatggaccag acaacacgat
    36721 taataatcca cgctctgaca tgacgacgtc ctttcaacta gtctttttgt acttatcgtt
    36781 tattttgcac tttttttctg caactttcaa ggttatcttt aattatagct agaaattggt
    36841 aaaaaagcta ccctgtacca agaggtcgta aaaaagctgt cctgcatcaa gtaataagaa
    36901 cagccaaaca taaacagctt ctcatgtttt tcgtttggtt ggggttatta tcgcagatgc
    36961 aagcttttga acgccgtgta taagaggtcg tgaagaagcc gattagtttc gagtatgaag
    37021 aaaaattttg tgaaaatagt ttctcacatt tttgcataaa tttggcttaa taccgagaaa
    37081 atggtttttg aacatcactt attcaaaata aaaaaagatt gtgacacgaa ttttgtcaca
    37141 acctttaaat gtattcctag agcaaagaag ctaagcgaag aaatccaatt ctgtttattt
    37201 attgtatttc tttgttcagc atccttgcta aaagcccata ttggacaaag tattcactac
    37261 acccatgatc tcatcttttc gagaacgtgc tttttctgtc ggatcttcta cctcttctgt
    37321 gtcaattcga acaataaatt tccgattcat cgtcacgtaa gcaccgtcgt tgatcaaaat
    37381 aaacagttcg ttctcattat agtctgcaca ggcatctttc catacgtcaa agccgttttt
    37441 ttcagaaggg atatcccgtt taaccacagt ttttccactt aattcgtgga acgttacgtc
    37501 ataatatttc attatctcta cctctttcgt cattcaatat tttgtacttg ttctctgatt
    37561 ttttctaaga tcgtcttaga ctggacgact tgatttttaa tttcgattgc atttgatttg
    37621 gaaccgatcg tgttgatttc ccgattcatt tcttggatca ggaaatctaa ttcccgacct
    37681 acaggacatt gtgcttcgat aagttcatct agtttttgta catggatcgc cagacgatcc
    37741 agttcctcgt gaatatctcc gcgttccaac aagatggcta gctccgtcaa caaccgt
//
LOCUS       DAZUKD010000014        37135 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000014 DAZUKD010000000
VERSION     DAZUKD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.014"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..936)
                     /locus_tag="ACG8N9_000579"
     CDS             complement(61..936)
                     /locus_tag="ACG8N9_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4896207.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1045..2814)
                     /gene="aspS"
                     /locus_tag="ACG8N9_000580"
     CDS             complement(1045..2814)
                     /gene="aspS"
                     /locus_tag="ACG8N9_000580"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4896208.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2829..4121)
                     /gene="hisS"
                     /locus_tag="ACG8N9_000581"
     CDS             complement(2829..4121)
                     /gene="hisS"
                     /locus_tag="ACG8N9_000581"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4896209.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4515..5462
                     /locus_tag="ACG8N9_000582"
     CDS             4515..5462
                     /locus_tag="ACG8N9_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4896210.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5551..6810)
                     /locus_tag="ACG8N9_000583"
     CDS             complement(5551..6810)
                     /locus_tag="ACG8N9_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4896211.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(6981..7427)
                     /gene="dtd"
                     /locus_tag="ACG8N9_000584"
     CDS             complement(6981..7427)
                     /gene="dtd"
                     /locus_tag="ACG8N9_000584"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4896212.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7438..9651)
                     /locus_tag="ACG8N9_000585"
     CDS             complement(7438..9651)
                     /locus_tag="ACG8N9_000585"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4896213.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9884..10648)
                     /locus_tag="ACG8N9_000586"
     CDS             complement(9884..10648)
                     /locus_tag="ACG8N9_000586"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4896214.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10649..11596)
                     /gene="prmA"
                     /locus_tag="ACG8N9_000587"
     CDS             complement(10649..11596)
                     /gene="prmA"
                     /locus_tag="ACG8N9_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4896215.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11609..12097)
                     /locus_tag="ACG8N9_000588"
     CDS             complement(11609..12097)
                     /locus_tag="ACG8N9_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4896216.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEAKQQNEADTYLPYP
                     SY"
     gene            complement(12063..12734)
                     /locus_tag="ACG8N9_000589"
     CDS             complement(12063..12734)
                     /locus_tag="ACG8N9_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4896217.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12872..14158
                     /locus_tag="ACG8N9_000590"
     CDS             12872..14158
                     /locus_tag="ACG8N9_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4896218.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14230..14424
                     /gene="ssrS"
                     /locus_tag="ACG8N9_000591"
     ncRNA           14230..14424
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8N9_000591"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14502..14786
                     /locus_tag="ACG8N9_000592"
     CDS             14502..14786
                     /locus_tag="ACG8N9_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896219.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14859..15338
                     /locus_tag="ACG8N9_000593"
     CDS             14859..15338
                     /locus_tag="ACG8N9_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4896220.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15560..15703
                     /locus_tag="ACG8N9_000594"
     CDS             15560..15703
                     /locus_tag="ACG8N9_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4896221.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16048..16176
                     /locus_tag="ACG8N9_000595"
     CDS             16048..16176
                     /locus_tag="ACG8N9_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896222.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            16281..17864
                     /gene="abc-f"
                     /locus_tag="ACG8N9_000596"
     CDS             16281..17864
                     /gene="abc-f"
                     /locus_tag="ACG8N9_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4896223.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18301..19485)
                     /locus_tag="ACG8N9_000597"
     CDS             complement(18301..19485)
                     /locus_tag="ACG8N9_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4896224.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19510..20517)
                     /locus_tag="ACG8N9_000598"
     CDS             complement(19510..20517)
                     /locus_tag="ACG8N9_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4896225.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20689..21048)
                     /gene="comGG"
                     /locus_tag="ACG8N9_000599"
     CDS             complement(20689..21048)
                     /gene="comGG"
                     /locus_tag="ACG8N9_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4896226.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21020..21445)
                     /gene="comGF"
                     /locus_tag="ACG8N9_000600"
     CDS             complement(21020..21445)
                     /gene="comGF"
                     /locus_tag="ACG8N9_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4896227.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21408..21755)
                     /locus_tag="ACG8N9_000601"
     CDS             complement(21408..21755)
                     /locus_tag="ACG8N9_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896228.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(21727..22197)
                     /gene="comGD"
                     /locus_tag="ACG8N9_000602"
     CDS             complement(21727..22197)
                     /gene="comGD"
                     /locus_tag="ACG8N9_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4896229.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22172..22450)
                     /gene="comGC"
                     /locus_tag="ACG8N9_000603"
     CDS             complement(22172..22450)
                     /gene="comGC"
                     /locus_tag="ACG8N9_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4896230.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22447..23496)
                     /gene="comGB"
                     /locus_tag="ACG8N9_000604"
     CDS             complement(22447..23496)
                     /gene="comGB"
                     /locus_tag="ACG8N9_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4896231.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23453..24382)
                     /gene="comGA"
                     /locus_tag="ACG8N9_000605"
     CDS             complement(23453..24382)
                     /gene="comGA"
                     /locus_tag="ACG8N9_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4896232.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTIW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24514..26268
                     /gene="ade"
                     /locus_tag="ACG8N9_000606"
     CDS             24514..26268
                     /gene="ade"
                     /locus_tag="ACG8N9_000606"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4896233.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26366..27028)
                     /gene="trmB"
                     /locus_tag="ACG8N9_000607"
     CDS             complement(26366..27028)
                     /gene="trmB"
                     /locus_tag="ACG8N9_000607"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4896234.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27098..27880)
                     /locus_tag="ACG8N9_000608"
     CDS             complement(27098..27880)
                     /locus_tag="ACG8N9_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4896235.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28084..29295)
                     /locus_tag="ACG8N9_000609"
     CDS             complement(28084..29295)
                     /locus_tag="ACG8N9_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896236.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29288..30025)
                     /locus_tag="ACG8N9_000610"
     CDS             complement(29288..30025)
                     /locus_tag="ACG8N9_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896237.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30163..30597
                     /locus_tag="ACG8N9_000611"
     CDS             30163..30597
                     /locus_tag="ACG8N9_000611"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4896238.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30590..30934
                     /locus_tag="ACG8N9_000612"
     CDS             30590..30934
                     /locus_tag="ACG8N9_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4896239.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31114..32124
                     /locus_tag="ACG8N9_000613"
     CDS             31114..32124
                     /locus_tag="ACG8N9_000613"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4896240.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(32193..33137)
                     /locus_tag="ACG8N9_000614"
     CDS             complement(32193..33137)
                     /locus_tag="ACG8N9_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4896241.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(33121..35841)
                     /locus_tag="ACG8N9_000615"
     CDS             complement(33121..35841)
                     /locus_tag="ACG8N9_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4896242.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(35838..37058)
                     /locus_tag="ACG8N9_000616"
     CDS             complement(35838..37058)
                     /locus_tag="ACG8N9_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4896243.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
BASE COUNT        10921 a         7744 c         6555 g        11915 t
ORIGIN      
        1 actgcagaca aacgaacgtc tgtctacagc agtatttcat gaatttttgt caaactcgat
       61 ctaaatgcca tcctcataga aacgaccaag tatctggacg ttttctgcaa tacttacaaa
      121 agcttgtgga tcagaagctt tcattgcttc tctaagtaga ggcagttcgt atcgtgtaac
      181 gatcgtcatc aatactgtct gcttatcatg cttataagca ccttctgctt cattgatgat
      241 cgtgatccct ctacgcatac agttttggat ttcatttact actttttctg gatgtttcgt
      301 tacgatcatt acctgcatct tcttttgttt agtataaacg acatcagtta cttttccact
      361 gacgaaaata gacaacgtac tgtaaaacat atattgccag ccaaatagaa ttccggcaac
      421 aaaaacgatc aatgcattga agtagatcga aatagaacca atcgactttc ctgttttttt
      481 acgaatcgtg atactcacga tatctagacc gcccgatgat aggccgtttc tcaacgctag
      541 cccaatacct agtcccatca cagccccccc aaacaacgca cagatgattg gatcggtaga
      601 taggactgtt tccggtaaaa tatggataca aagggaggtc aaagtgactg tgataaaagt
      661 gaaaattgta aacttcttcc cgatttttaa ccacgccaat acaaacaaag gtatattcag
      721 taaatacaaa gttgttgata ccggtacttc gaatcctatc agtgattttg acaaagtcgt
      781 caaaatctga gctagaccag taataccact tgaataaata tgtcctggct gataaaagaa
      841 attcaatgcg actgatgcga tcaacgcata aacgatagat accgaaagct tggtcgtgta
      901 atcatgataa gcccaatttt cgaatgtttt tttcatggaa aagccccgct ttatatgtta
      961 tggcttcatt ataaagtgtt tttcaattaa atcaaggttg tcaaaagaag cgtattcgac
     1021 aaatcctttt taccttttta tcggctattc ttctatattc gttacatcaa tattcaattc
     1081 gaacaattgt aaaggcgaaa ccatacttgg tgcatcactc atcacatctg ctgcttttcc
     1141 gtttttagga aaggcaatga cttcacgaat attatcttct ccggctaata acattgcaaa
     1201 acggtccaat cctaatgcaa taccgccatg tggagggaat ccgtaatcta aagctgttaa
     1261 caagaaacca aattgttctt ccatttgttc ttttgtaaag cctagagttt ctagtacttt
     1321 ttcttgaagc tcacgagtat gaatacggat agaacctccg cctaattcat aaccgttcaa
     1381 gacgatatca taagcttctg catagacttt tgcagggtct tcagccaata acgggatatc
     1441 ttcttctttt ggcatcgtga atggatggtg agcagagaca tatcgacctt cttcttctga
     1501 aaattcaaat tgtggccaat cagttaccca taagaaattg aatttcgatt catcaattag
     1561 tcctaattct tttcctaaac gtgtccggat agcacctaat gcagcagcga cgatttcact
     1621 tttatctgcg ccaaacatca gcaagtcacc tggttttgca tctgttgctt taatgattgc
     1681 ttctgttgct tctcccatga atttagcaat tggacctttc aatccgtctt cttctacttt
     1741 cagccatgca agccccttag ctccgaattg tcctacatat tgacctaact gatccatgtc
     1801 ttttcgtgag tagcgatctg cagcgccttt agcattcaat gctttcacga caccgccgtt
     1861 ttcaagagcc atttggaaga ctttgaattc tacttctttg actgtatcgc ttagatcgat
     1921 caattccata tcaaaacgtg tatccggttt gtcgctgccg tagcgagcca ttgcttcatc
     1981 ataggtcata cgaggaaatg gcaatgtgac ttcgataccg cgaacttctt tcattacctt
     2041 cgcaatcaat ccctctgtgt aagtttggat ttcttctgct gttaagaacg ttgtttcaat
     2101 atctacttgc gtaaattcag gctgacggtc tccacgtaaa tcttcatcgc ggaaacaacg
     2161 aacgatttga taataacggt cgaatccagc attcatcagc aattgtttaa acaattgagg
     2221 agattgcggc aacgcataga aatgtccagc atggacacgt gaaggaacta ggtagtctct
     2281 tgcgccttct ggtgtagatt tccctaggta aggtgtttcg atatcgagaa agtcattatc
     2341 atctaaataa tgacgaatcg cttttgttgt cgcatttctt aatttgatgt ttttagtcat
     2401 ttttggacga cgcagatcca aataacgata tttcaaacgt atttcgtcac tgattgcttg
     2461 atcatcttca atttgaaatg gcggggtttt tgcttgattc aaaatcacga tttcacttgc
     2521 aatcacttcg aattcaccag tggccatttt aggatttact gcattttctc cgcgtcttct
     2581 aacttcccca gtcacttcga tcacgtattc actgcgacat ttatccgcga tttcccatgc
     2641 ttctttgttg atttcaggat taaagacgat ttgaacgatt ccttcacgat cacgtaggtc
     2701 gataaagatt actcccccaa ggtctcgtct tttttgtacc catcctttta atgtaactgt
     2761 ttgttctaat tgttgttcgg aaactaatcc gcaatagatc gttctttgtg ccatttattt
     2821 ttttcctcct actcgaataa agtcatttca tcatagactt catcaaaatg ttcatatatc
     2881 tcttctaatg gaaaactttt ttcttttcgg cttgccattg ctttgacatt aatcgtttga
     2941 ctagcaagct catcttcacc gatcaccaat actaatttgg cattcgcttt atctgctgtc
     3001 ttaaattgcg cttttgcttt acggttcatg aagtcgcggt cagctgaaaa accaaagttt
     3061 cgaatcgctt ggaccacttt caatgcttcg atatttgttt cgtctccgag tgcgaccacg
     3121 taagcatcca actcattaaa tgcagggata actacttcct ctgcctccat agtcaagagt
     3181 aatcgctcga tacccattgc aaatccgaag ccaggtgttg aaggaccacc tagttcttcc
     3241 actagattgt cataacgtcc gcctgcgcaa atcgttgctt gtgcccccat ttttggtgcg
     3301 tcactcatga tttcaaaaat cgtatgtgtg taataatcta gtccgcgaac catattactg
     3361 tcgatttcat aaggaacagc caatgcatcc agcatggaaa caacggtatc aaaatgtttt
     3421 tgagcaggct cgcttagata atctaagata gatggagctt ctgccacaaa tttttgatct
     3481 cgtttgtctt tactgtccaa tacgcgtaat ggattttcat gaaggcggcg tttagagtct
     3541 tcactcagct ctgattcaaa aggtaaaaga tagtcgatca atgcttgacg ataggcttga
     3601 cgcgtttctt tatcacctaa tgaattgatg actagccgga tttgcgtgat ccccaactgt
     3661 ttgaagaaat caagtgccat caccatgctc tccacatcag ttgcagggtt ttctgatcca
     3721 aatgcttcta ctccgatttg atggaattga cgtaaccgtc ctttttgagg gcgttcgtag
     3781 cgaaacattg gtcccatata aaaagttttg aacggtttcg catactctgg gccgaacaat
     3841 ttatgctcaa caaaagaacg aacgattggt gctgtacctt ctggtcgaag cgtcacatgg
     3901 cgttcccctt tgtcataaaa atcatacatc tcttttgaaa cgatatccgt tgtatcccct
     3961 acacttcggg atatcacttc atagtgttca aaaatcggtg tacgcatttc attatactga
     4021 taatctctga ataataaacg tgctgtttct tcgacaaact gccatttttc tgattcgcct
     4081 ggcaaaatat catttgttcc ttttggtctt tggaaactca tagttttctc tccctttctt
     4141 agctatgtaa ataaaaaaat cacccttgtt catcatgaac aagggtgaat gaaattcata
     4201 cacggtacca cctaattttg aaagatttct ctttcctctt acgaataacg ctcgtacgcg
     4261 ggacggcttt tcactcgtcc atctccagag tgtctttagg ctaatgaaac tttagatcat
     4321 tttcagcctc gatgatcctc tcttgtaaag tttcaaaaac ttactcgctc tttcattgac
     4381 attcgatatt acttataacc tactaaaaac cagtctcaaa gtcaagagat atctgactat
     4441 ttcccctcac aaaaacgttt tagttcagct ttttggtatt ttgttttaga ctagacaaaa
     4501 aggaggaatc agctatggca gaaataagaa tcggacattc acaagtctac gcagaacagc
     4561 ttggattagg agcaaatgcc gttggaggac ataatctttt tgacggtttg gaagatgaga
     4621 caggaaaaca agtagttcgt acagctttga atagcgggat caatttgatc gataccgctt
     4681 atgcttacgg aaacggccgt tccgaagaac tgattggcga ggtactgaag gaaaaagaat
     4741 atgatcgctc acgtgtcgtc atcgctacaa aagctgctca tgtaccaaat aaagggcgaa
     4801 catttgataa ttctcctgag tttctcaaac aatcggtgga agatgcacta aaacgtctgc
     4861 aaactgatta catcgacatt ttttatatcc actttccgga tgaaagcaca ccaaaaaatg
     4921 aatcagttgc tacattgcac gaattaaaag aagcaggaaa aatccgagca gtcggtgtgt
     4981 caaacttcac gttagaacaa ttgaaagaag cgaatgcaga tggatatgtc gatgtagtag
     5041 aagataaata cagtttgatc catcggcagg ctgaaaaaga attattccct tatcttgaaa
     5101 aaaacaaaat cagttttgtt ccctattttc ctcttgcttc cggtttgctg acaggaaaat
     5161 atgaacttgg tgaggaaaaa caatttggtg aaggagatcc tcgaaaaaga aatccagatt
     5221 tccaaggaga acgtttcaga gaaattttaa ccgctgtcga tgtacttcgc cccattgcta
     5281 aaagatacca agccactcct gcgcaacttg ttttagcttg gtacatgaag aatccacgag
     5341 tgtctgtagt tatacctgga gctaagcgtc cagagcaagt atctgacaat gtccaagctt
     5401 tagatttgca tttatccaat gaagattatc aaaccattga tgaagcattt cgtggatttt
     5461 gatcaattga ccttatcaat ttatagtaat atgaaaaaag gaagatcagc actgtttgat
     5521 cttccttttt ttcgataagt ttatgctaat ctaattcttg taaagtagcg ctcggctatt
     5581 cggataagtc ggaaactgat tgaatggaat cgtccaatct tgttcaggta cagagaactc
     5641 aaacttattt actagctgat cggaaaacac acgtagacta tgaagagtga tccattctcc
     5701 tgcacagcga tgcatttgtc gaaaatttcc cccgccttgt gcaatcatct catattcttc
     5761 tttataggaa atatcttttg ctttccctac ataccgtttg atcataaagg aatctggcgc
     5821 ttcaactgtt cgttcatcat ggtctgttcc atataaatct aaaataaccc aactgccttc
     5881 tggtatacga taaccatcta cttctacttc tttcagcgaa atcgcaggta gcattgggaa
     5941 gaaaggataa tagcgtctca tttcttgaat aaacggatct tgtaacgtcg aaaagtcatt
     6001 ttttaattgc tgatacaaat ctggtctact gaaaagtgca tgtcccatca atgccgccca
     6061 cacagttaga gcaaccgttg gacggatgat gttcaataat tcaacagctg ccacttccag
     6121 aggtaaaagc tgaccatcta aatctgtggc attggcaaaa gcatataatg ccacattttc
     6181 ttttccagcc acaggatttt gtcgagcttc ttcaatcaag ccttgcgccc atttttctga
     6241 ttttttgcga ttctcgacac cttttatgtg atcaatcgga gaagtaaacg ttccgctgat
     6301 catagagatt tgatattctg ctaatttttc gacctcatct ttagaaatcg ctgataaatt
     6361 gatacctgcc cactcgcaaa tagatgtaaa gaaaaccatc ttacttaaat caaatagttc
     6421 aaattgacca tgctgtgctt caagtgcttg tgtcagattc ttatctaaga tacgatgata
     6481 atcttccatt ctttccggtg tcattagatc cataaaaata tttttccgat ggtgatgagc
     6541 agctccatcc aaagtctgta ctccaccttg accaaaaagt gtcttcaata ctaattttgg
     6601 catagcacct tttcgtttga aattccttgg atcataaaat acttttgctg ctgatgatcc
     6661 gtagatcgtg atagcttctt tattgaaaat ctttgctttg acgacgggag cgtccgcttc
     6721 atggcgtaac tcctccaaca tgttgtatcc tttttgatac aattttttta aatctgtgat
     6781 tttgatatct actactggta cttctttcat cttcagcaac ctcctataaa aaataacttg
     6841 tataatttaa gcttaacttt ttttccgact gctgacaaaa gaagcgcttt ttgcagacaa
     6901 aaaagccact taagttagag tttatcagtc taacttaagc gacgtgtttc atagttttta
     6961 ctagtgaaaa ccagattact ttatttgttt ttagaatcaa taatgatcgt tacaggtcca
     7021 tcattgacga gagaaaccga catatctgct ccgaattgtc cggtagcgac tgtgatgccg
     7081 cgacttctga ttccttcatt gaatgattcg tataaaggaa tagctgtttc tggtcgtgcg
     7141 gcttccacaa agcttggtcg atttcctttt ttcgtatccg caaacagtgt gaattgagag
     7201 atacttaaaa tttgtcctcc cacatcttct acactaatat tcatttttcc ttgttcatct
     7261 tcaaaaatac gcatttgtgc aatttttttt accagatagt ccacatcttt ttgcgtatcg
     7321 gtttcatgaa tgcctaataa aacaagtagg ccgtagccaa tttttccaat ttcttgctga
     7381 tcgactgaca cagcagcttt acttacccgt tgaacaactg ctctcatatc tgtcctccta
     7441 tccattcgtc cggcgtacac tgtagacatc aggtactgct ttgattttat ccacgatact
     7501 ctttaggtgg gataaattct ggataccaac cgtcaaatgg atcgtcgcca ttttatcttt
     7561 attcgtccgt gcttcaacgc tgttgagatt tttcgttagc gcattgactg tttgaagtac
     7621 atcattcaat aagcctgaac gattatagcc gtaaatttcc aaatctgcat catactcttt
     7681 tcgtgtattc gatgtatctt cccattcgac ttcaatcaaa cgttctgcta catttggttt
     7741 gtcaggctga acattcggac aatctcgacg atgaatggat atccctctgc ctttagtgat
     7801 ataaccaacg atatcatcac caggaatcgg attacagcag cgactgatac gaattagtaa
     7861 gttttctacc ccttgaatga caacgccacc ttcatgacgt actttcattt tgtcaggttc
     7921 ttttttcggc tgattcatga tttcttctgc ttcttgcttt tgctgctcga ttttttgttc
     7981 tttacgttct ttttcagtca gacgattggc catcgtcaag gggctaactt ctccataccc
     8041 tacagctgca tagagatcat cttctgtttg ataattaaaa cgatcgagtg cttcttgcag
     8101 tttgttcttc gtcaaaatat ctttaggcgt aaatcctaga tctgtaatac atttgaccac
     8161 ggattcgtgg cctttgatca cattttcttc tcgatcttgg gctttgaaga aacgtttgat
     8221 cttatttctt gctttgctag tagcaactaa tttcaaccag tcgcgacttg ggccaaatga
     8281 atttggagaa gtcatgatct caatgatatc tccgtttttc aatttgtaat caagctgcac
     8341 cattttgcca tttacttttg caccagtggt tttgttaccg atatctgtat ggatactgta
     8401 tgcaaagtcc aatggtccgg atccttttgg taactcagta acgtctcctt ttggtgtgaa
     8461 gacgtagact ttatcactaa agatatctcc ttttacgcct tccataaatt cagaagcatc
     8521 atagctttcg tcttgaagtt cgagtatctc atggaaccag cttaattgtt tcgtcatacc
     8581 atctggttct actttttcat tttttccttc tttataggcc cagtgtgcag caaccccgaa
     8641 ttcagcgatt tcatgcattt cctgtgttcg gatttgaatt tctaccggat ttcctgccgg
     8701 accgatcacg gtagtatgca aagattggta catattagct tttggcatgg caatataatc
     8761 cttaaatctg ccgggcattg gtttccattt tgtgtggatt gctcctaata cagcataaca
     8821 atccttgatt gaatctacga tcactcgaat agctagcaag tcataaattt cgttgaactg
     8881 ctttttctga tctttcattt tacgatagat tgaataaatg tgttttggcc gtccatagat
     8941 ttctgcaaaa atccccagct cttctgtcgc tattcgaata tcttcaacag taccacttac
     9001 gtatttttca cgttcttctc tcttcgtctg catcaagtgg acgatacggt aatactgttt
     9061 tggatttaga taacgaagtg ctgtatcttc taattcccat ttgatccggc tgatccctag
     9121 acgatgtgca agaggcgcat agatttctaa agtttcctga gcgatccttc tttgcttatc
     9181 ttctcgcagg tgcttcaacg tacgcatgtt atgtagtcta tcagctaatt tgaccatgat
     9241 aactcgtaaa tcttgagcca ttgctagcag catctttcgg tggttttctg ctagctgctc
     9301 ttcgtgggat ttatatttta ttttccctaa tttggttacg ccgtccacta acatagcaac
     9361 gtcatcgcca aattcttctc tcagatcttc taaagtgatt tctgtatctt caacaacatc
     9421 atgtaagaag cctgtagcca ctgtatgggg atccattttt aaatctgcta atatacctgc
     9481 gacttggata ggatgaataa tgtagggttc gcctgatttt ctaaactgtc cgtcatgtgc
     9541 agcagtcgcg tattcgcatg ctttctcaac aaatgctaca tgttcgggac ccatgtattg
     9601 actaacgagc tttatgacac cgggtcccgt taaaatttct tcttttgtca caggaatcga
     9661 tccccctttc ttttttcggt aaaacgggaa tcgggacaaa tatctgtttg ataattgtcc
     9721 caaaatccgt gcagttatac gtttttttac ggaaatgttc gctttttaaa agctttttca
     9781 aacgattttt cgtatccatt gtataaataa ttgtttctat tatacaaatt gttgactatt
     9841 ttttcaattc tttctgtcga tactctttta ttaatgaaga acgttagctg aaagaaggat
     9901 tcattaattc ccattgatag ctagcagcac ttaataaata aagaggtgct gtttctgtac
     9961 gaaggattct tggtcctaat ccacaaagta tccctttgtg ttctacaaaa taatcgactt
    10021 catcgcggga taatccacct tctggtccaa agacagctaa gattcgacta tctggttttg
    10081 tttcactaag aattttggct gcatttgatt tttcgccttg ctttgctgat tcctcataag
    10141 caaccaagac cacgtcataa tcatctagcc gctcaaataa atcttgtttc gtttccaata
    10201 actctactga tggcacgatc tgccgatggg attgttccgc tgcctcttga gcaatctttt
    10261 caagccgttg ttgtcgtttc atacgttttt ttgcatccca tttaacgaca gaggctgatg
    10321 ccgggaaacc aagaaaagct tgcgctccta attcagtgga tttctgaacg atccaatcca
    10381 gcttttcccc tttaggatag ccactcgcta tggtaatcga acttggcagt tccttttgat
    10441 tttcttcttt tttaatttct ttcagataaa cagcctcttc agtaatctcc gtgatttctg
    10501 caacaatggc tatttgatca ttgaatgcca aaaaaacttc atcctcgggt tccatcctca
    10561 taacccgtac aatgtggtga tagttttccc cttctaaaac aaaatagtct tttgattcat
    10621 atgattcttt taaaaaatac cgctgcatct attccacctc tggttttttc aaaataattg
    10681 cataccagtc tttttgctgg aaaatttgat ccacgataaa cccttgttct aacatggcat
    10741 ccagaaccat ttgcttcttc tcttcaatga ttcctgaaac gatcagcgtg cctgtttgct
    10801 tcagtaaacg ccaagcatct gggatcatca ataagatgat atctgctaaa atgttcgcaa
    10861 cgattacatc actttcaatc tcgattcctg tcaaaagatc gttagctgat acatggacat
    10921 ctttggctat ttcattcata tccatatttt ctttcgccga acgaacggct acttcgtcta
    10981 aatcaaaggc atagacatct tttgcaccaa gatattttgc tgcaatactt agcacgcccg
    11041 acccagttcc cacatctagc aatgtttcac caccacgcaa gactgtttct agtgctgtca
    11101 atgtcaagga agtcgtggga tgagtgcctg tgccaaatgc cataccagga tctagtgtga
    11161 taattttttc tagaggatct tctgtctggt agttttccca gcttggcgca accgtcagca
    11221 ttcgcgtaat acgcaccgga tgatagtatt tcttccaggc tgttgcccag ttgctttcct
    11281 ctacttcact aactgttact ttattttttc caatagctag accaaactca ggtaattgtg
    11341 tgatacgatc tttgataaaa ggcaagattt ccggtaagaa agtcgtttcc ggaaaataag
    11401 ccatgactag tgcgccttct ttcagcgatg taaaatcatt tttatctaaa atctcaccat
    11461 attgatccga ctgaaaatgt tcaacatcta aagaatcttc aatcgcgaca ccactcgccc
    11521 ctgcctccat caaaatatta gcaactgctt cgactgcttc actttcagtt tctactttta
    11581 cttcattcca tttcatatct gtcactcctt aataacttgg gtacggtaaa taagtatctg
    11641 cttcattttg ctgttttgct tctactaatt ctgctagttg ttgattatcc cagtgaagct
    11701 cgaatacagc ttcgtcatcg tcttcatcca aaaaatcaag caaatcgctt tgcccttgag
    11761 ctaatacttc atttagatat tccactaaag aatcagctac tgattttgcc aacccttttt
    11821 ttccttcata agggattact gctagataat tgcgttcatc aaaaacagat ttctgaggat
    11881 taaaaagaag tacgccgtct tcaaattcga taattggttc ttgcgaaacg atcccttctg
    11941 catcatcaat ctcaaattgt gctttatttt ctgcaaacaa gcgaacaacg atctcgatcg
    12001 tatgattttt tgtatcccaa tctaaagcta catcatagtc tgtcacttgt ttttcaatta
    12061 tttcatctaa atatgttaat agatccttct ttttcatttt ctttcctcca accccaatct
    12121 ttctcgattt ggttttttct ttgtaaagag atataaggat ttcccgacac aacaaaacgc
    12181 aacggctttt ctgtccatac acctttgttt gggataccga ttctaggcaa agcattgatc
    12241 ttttttggtg tctttttttt ctcaaaaact aagcgtaacg aactatctgc tatagactga
    12301 ccataaagtt cttgaggaat tgctaatgct tccactaatt ttcccggacc gttgctgata
    12361 tctggaccag tttttccgcc acgattttta ctcatttgat caatcattgc ggcgggttcg
    12421 attgcacgta tcattactcc ttgaggaatc ccttctggct gagtaatgat gttcaaaatc
    12481 cgatgagtat gcatcgtgta aaggtagatc gtacctggtt tttcatacat tgcccgcaca
    12541 cgcggtgttc ttcttaaacc atagctatgg gcggcctcgt cttctggacc taggtatgct
    12601 tccgcgtcta caatatatcc agctaatctt ccttctggtg tgtcatgctc caaatacatc
    12661 cccaataaat cccgtgccac ttctggcgtc gttttattcc aaaaaaattc atctatcttt
    12721 tgtttatccg tcatttttct cacctacttt atcatacaca atattagttg gataagctat
    12781 gaaaacaaaa caacagaagg ctgttctttc taggcatctt gctaaatatg atataattaa
    12841 gggacgtaaa aacaagaagg aggaaaatcc tatgcaacag ccattggctt atcgcatgag
    12901 acctcgaacg atcgatgaag tcgttggtca gcagcatcta gtcggggaag gaaaaatcat
    12961 acgccgcatg gtcgatgctc gcatgctctc ttccatgatt ctatatggac ctcctggtac
    13021 tggaaaaacg agtatcgcaa gtgcgatcgc cggctctact aattatgcat ttcgcatgct
    13081 aaacgccgct acagattcaa aaaaggatct acaagttgta gcggaagaag caaaaatgag
    13141 tggtacagtg atcctcttat tggatgaagt acaccgatta gataaaacaa aacaggattt
    13201 tctgcttcct catttagaaa gtggccgaat catcttgatc ggagcgacta ctgagaatcc
    13261 ttatattacc atcaatccag cgatccgcag ccgtacacag atctttgaag tcaaaccatt
    13321 aacagaacaa gacatccagt tggcagtcga acatgcttta agggataaag aacgcggcct
    13381 tggacaacaa gcaatccagt tagacgaaga tgctcttctt cacctttcgc gtgcaacgaa
    13441 cggcgatttg cggagtgcat tgaatgggct ggaactggct acactatcga ctccttcaga
    13501 taaagaagga aggattcatc ttacactgtc gatcattgaa gaatgtgtcc aaagaaaagc
    13561 tcttacccat gacaaaaatg gagatgctca ttatgacgtt atttcggctt ttcaaaaatc
    13621 gattcgtgga agtgatgtgg atgctgcact tcattatctc gcccgtttag ttgaggcagg
    13681 tgatctggca agtatttgcc ggcgtctgat ggttatcggt tatgaagata taggtttagg
    13741 taatcctgct gctgcggctc gtacagtgaa cgctgtatta gctgctgaaa aattgggatt
    13801 gcctgaggca cgtatcccac tagcagacgt cgtcgtcgat ctttgcttgt cgccaaaatc
    13861 caattctgcc tacatggcat tagatgcagc acttgctgat attcgagagg gcaaagctgg
    13921 agatgttcct gatcatttga gagatagcca ttacaaaggt gcgaaatcac tcaaccgtgg
    13981 cgtgggttat cagtatcctc atcattttga tcaggcttgg gtcaatcagc aatatttacc
    14041 agataaactg aagaatgctc agtattatca gccaaaagat acagggaaat atgaacaggc
    14101 tctgggtcaa cagtattacc gtataaaaga gtggaaaaag catcctccaa aaaactagac
    14161 aagaaaacgg ttctgtttct gcgagctctc tcattgctct ttttaaattc atatgctact
    14221 atagaggtgg gaaatctgtg atgttcgaat tgtcctcttt gtgtgttgac cgaacatttt
    14281 tattgatatc ttgggacccg tcttgtgtca ctgctggtaa catgccttat catttggtag
    14341 ttcgaagcta cgaccatggg acccacctgc ttgaattcgc gggatcaata ctacaggaca
    14401 aacaacggca ttaacggaga ttaccatctt ttaaagaata aagcaatttg ctcttggcaa
    14461 attgcttttt ctatttgttg cttattttgg aggatttgtt catgattagg attttaatga
    14521 ttatcgccac tttagtatta ttattcgtta gctattattt atttaataaa caggatatct
    14581 tttttgtatt gatcaacaaa aatgataaaa atcaaggatt cctacaattt tacggtgctg
    14641 catatgctgt tttaggcgta atgggtattt taacagcttt tttcaatcaa cgctttattg
    14701 ccttggtctt tttactgatc gtaattattg tttctgccac cttcagtatc cgatttgcaa
    14761 aaaaaattgc tgaaccaaag cagtaaataa ttctcttttt ccctaaagtg ttctacaata
    14821 aaagagtaca atacgattga cttgaggagt gaatgcttat gttacaacaa tacaaaaaaa
    14881 tcatggttgc cgtagatggt tctgatgaag ctgaattagc ttttaaaaag gcggtcaatg
    14941 tagctatccg aaataacgga gaacttctat tagcacatgt cattgacaca cgttctttcc
    15001 aaaccgtttc atcctttgat ggaatgcttg cagaacaagc tactgaaatg gcaaagcaaa
    15061 cattggcaga ttatgaaagc aatgcgaaga aagctggctt gaataatgta acctctgtcg
    15121 tagaatatgg ctcaccaaaa caaattattg ccagagaaat cccagaagat aatcaagtag
    15181 atttgattat gttaggagcc acaggattga atgctgtaga acgtttattt attggttctg
    15241 tttctgaata tgttattaga aacgcagctt gcgatgtgct tgttgtccga actgatttag
    15301 aaaaccaatt gccagctaaa gaccacacca aagcttaaca cgtagacgac ctgccaacta
    15361 tcggcaggtt tttttgcaca aaaaaccgtg ataaaaacaa aagacgcggc aagcactcat
    15421 cggtctttat aatgtttaaa ataaagggaa aacagtcaat gagttcaagt aaaataaaaa
    15481 aagtgaacac cagggcgatt agtcgccctg gtgtggttta aaacgaccta agcatgccaa
    15541 agcttattgg ttcgtcaatg tggaagtttc cgatgaatct gatacgaaaa aatatgaagt
    15601 atcgatggcg gataccgatt ttatcgccaa actaaaattt tatttcgggt tcgggtaccg
    15661 cgagaaggca agtgctacat tcggtaaatc attcaagaat tgatgaaagg ctaccgcttg
    15721 ccattgcaac cacaacgata ctcctgaacg cgatgttggt gagatttcca aagagaagag
    15781 ctgtttaatg caacggtatt aagcaaatgg caaaaggaag tcatgtagac aaagtttttt
    15841 atcaaacacc atatgatttc gcctgaacgc tggtttagct cagaatacaa tgatgtggtc
    15901 cacgagacat taccctcagt cagataaaaa aagctctctg acagaattac acatcgataa
    15961 atattaagta aatgtcagcg ctagttgaac tttctgacac tttcaggtac actggaaaag
    16021 acaactacac gaaggaggga aaatactatg atgcgcatga ctaaaataac aagtaatgaa
    16081 aattaccaac gataccatat cgcatttagc catgcaattg gttttacagt tgctgttgac
    16141 gggacaagtt tgtccaaaac agttgaattc acgtaaaatc aatggccatc tcaaggtatt
    16201 tttctcatga taatctgagt ttaaataaca caggccaggg ataaccatgg cctttttgag
    16261 tggtctcaag gagagagatc atgatcatac aagcaatttc tattaaaaaa acaatcggcg
    16321 ggaatccatt atttgaggat ttatcctgtc aaattgaagc tggtgaaaaa attgccttag
    16381 tcggtatcaa cggaactggc aaatcaacct ttttacaaat cttgaccggc agagaaggtg
    16441 tcgacagcgg tgttatcagt cgcaaaaaag gcttaaagat cgggacggtc gaacaagaac
    16501 ttacagtttc cgaagcgacc gtcaaccact acttgctcca ttctgccgct gagatacagg
    16561 acctcaaaca gcaaatgagt cgctacgaag cattgatgac agaaccagat acaaacttag
    16621 agaagtgtct ggcccgttac ggtgagctgc agcatcgctt tgaagaacta ggcggttacg
    16681 tattagaaga ccggattgca gcgatcttgc aaggcttagg gattccacac cgtcaggaag
    16741 cacggttaac agaacttagc ggaggcgaac gagttaaagt cgctttagcc aaaattttag
    16801 cagccgatgc cgatttactt ttactcgatg aaccgaccaa tcatttagat cttaacagta
    16861 ttcgctggtt ggaaaactat ttacagaata caaaaaaatc ggttctggtc gtttctcatg
    16921 atcggcaatt tctcgatcaa gtcaccacaa aaacatggga actggaagaa ggtgcattga
    16981 ttgagtatcc cggaaactac agccgttttc gagtcttgaa agaagctcgc ttggctgagc
    17041 tgacaaaaaa ttacgaactg cagcaaaaag aagtccagcg gttgaaagtc atgattcgtc
    17101 gttttcgtca gtgggcacat gaaggagaca atgagtcctt cttcaaaaaa gccaaagaac
    17161 tggaacgccg cctggcgaaa ctgacactcg tcaaaccgcc acctcccccg aaaaatcggc
    17221 tgcaaagctt gtctaatggt ggaaaatctg gcaaagaagt tttcatcatt caaaacttgc
    17281 accagcagta cgctgatcaa gtactattca aagacagcag ttttgcagtc tatcgtggag
    17341 atcatttagc catcatcggc gacaatggtg caggaaaatc aaccttactc aaattattac
    17401 tcggtgagga aaagccttac ggtggcacca tcaaattagg tagtagcttg caaatcggtt
    17461 atttgccgca gcagctgcag tttgccgatc ccgatagccg tttgctggct tacgccatca
    17521 cattgactgg aaacgaagaa gtcgctcgac ggcaattagc caaaagtggc ttttatcaaa
    17581 gcgatgtggg taaacggatc aaagatttat ccggtggtga aaaaatccgt ttggcgttgc
    17641 tgaaactctt tttagaaaaa atcaacgtcc tgattttgga tgaaccaacc aatcacctcg
    17701 acagctatgc tcgagaagaa attgaagaaa tgctgcaaga ctatcaagga acgctcctag
    17761 cagtcagcca tgaccgctac tttttgcagc aacacttcca agaagcactc gttattgatc
    17821 aggagcagat cagccgccag ccgctcggcc aactgattga ctaggacgtt ttttcgcaac
    17881 aaaaaacttg attttcatac ctgtgctttc acgattcagg caaagtacag gtggaaaatc
    17941 aagtttttat tattcgatca atgaatgttt cggatcggtt ttctagctaa acataaagcg
    18001 aagaataaag tcgacagccc acgaggctga tctaaaaatg ttttttcttt ttacatatgt
    18061 aatgtccctt ataaagatga atgaaaataa aatattggga ttttcctgaa aatcaataaa
    18121 taaaatgcca gtcttttccc ttttgaccac ttttccaagc aaaattccct aagaagatcc
    18181 tgtaaaaaag aagggaaatt gaagttaaat taccattttg cggtcttctc cgcaaaaaaa
    18241 acacatgcaa gaattccctc ttgcatgcgt ttcatctttt atcctaacga atgctttggc
    18301 ttagccgcgc agacgctcca catcacgggc aatcattagt tcctcatctg ttgggatcac
    18361 taatactctt acttttgatt cttttgtaga gatatctact tcttcaccac gcaattcatt
    18421 caacgctgga tcgatttcac aaccaaacca agacatacct ttgatgattt cactacgtac
    18481 atgtccgtca ttctcgccga taccagcagt aaagacaatt gcatcaacac cattcatcgt
    18541 tgtcacataa ccgccgatgt attttcggat gcggtccacg aaaatgtcat aagctaaacg
    18601 aatatgttct ttttcatagt tcttttcaag atcacgcata tcactagaca atcctgttag
    18661 gcctagcaga ccagatttct tgttcaaaac attgatcatt tcatcaatat caatctctaa
    18721 tttttccatt aaatatggga gaacagctgg atcgatatca cctgaacgtg tacccattgt
    18781 caccccagct aatggtgtga atcccataga agtatccatg gatttacctc catcgactgc
    18841 tgtgatcgat acaccatttc ctaagtggca agtgatgatt tttagttctt cgatcggttt
    18901 gttcaataat tttgccgcac gttccgacac ataacgatga cttgtgccat ggaagccata
    18961 ttttcttact ttgaaatctt cataataaga caccggtaag ctatataaat aattttgctt
    19021 tggcattgtt gagtggaaag atgtatcgaa tacagccaca ctcaaaatgt caggtaaaat
    19081 ctttttaaac gctttgatcc ccattaagtt tgcaggatta tgcaaaggag caaattctgc
    19141 taattcagca atgcggtcca ttactttctc atcgatcact actgaatccc cataagtttc
    19201 tcctccgtga acaacacggt gccctactcc ggtgatttca tcataagagc ttaaaatttg
    19261 cagttcaatc aactgatcta acagcatttt tactgctact tcgtggtttt cgatatccat
    19321 gatttgttcg aatttttgat tatcgccgta cttgatggta aaaatagaat cttgtaaacc
    19381 gatacgttcg acgatccctt tcgcaactac ggtttcttct ggcatttgat ataattgcca
    19441 tttcaaactt gaactcccag cattgattgc aattgtttta gacatatgac tctccttctc
    19501 gttgaatgat tacagagcag atgatttcca gtttttaaac tcgttgaaaa attccgtcac
    19561 cttctctggt tcttttaatg aagcaagttt cacaagcaag acttctttta cttgttgtgc
    19621 ttgtggtcct tttttctgta agatcaaaat acttttttgc gattgtttat tacggaacaa
    19681 ttcatctggc aattgaatca ttccttgaag ataaccttct tctttaaacc attttttgat
    19741 ttcttcactt tgatccgttt ctaaaaatcc actcggcatc aaaaacaaac caaaaccatc
    19801 cggcttcaca tatttcattg attgctctag caataaatga tgtgcataac tatggccttc
    19861 ttcggtactt gttaaaaact cttttgcttt ttcgtcattc ggataataac cgattggcaa
    19921 atcgctgatt gcaaaatcga caggatctat taataattcc tgcaacccgt cttgatgaaa
    19981 gtattgaaca tttgcttgtg tcaaatcact tgtagaagct gcgacagcca acaatgtatc
    20041 atcaatatcc acaccaattc cttctgtttg gtaccccgca ttcgataggt ttaaaagaac
    20101 tgttaataac aagttgccca ttcctgccgc aatatccaga atcttaaccg gtgctttttt
    20161 atttgtgtat agttgttcaa taagaaaaac aaataaaaaa ccgatactat caggagtgag
    20221 ttgatggttc gcctgcaaat gttctgtctg gctgcccttc aataaaagta attgagacag
    20281 tctgcgccat tcttctggct cgaaagaaag tttcttcaac tcttcataaa gagctgtgat
    20341 ccgttgcgtt gtctcttttg tcggcactcc atccaccaca cgtacttgat aatcatctat
    20401 taaattttct gcattttcga cataggcttc taaaaaggaa gttcccaatg cattctgtaa
    20461 aagctgaata gcttccaggt tttgattaaa cccttgttcc attttttctg gtaacatggt
    20521 gcacctcatt tctagatata ttataacaat tttttcacct gtttaattct atcgaacaat
    20581 tgacaaaatt tcaatcgttt tcactgtttt ttatttgtga aacggcaaac atttttttat
    20641 taaaaacagt gttataacag aaaagagggc gttttcacga acattccatc attctgtact
    20701 agtatctgta tcatttttta cttcttctgt gaaccgaaag ataaacggtg agacttccac
    20761 ctctaagaat agggactgtt gtacccactg atatgttaca gatccagttg tgtagtaaaa
    20821 aactccttcc gaactttcgg caagcgtaga ctgttccgat aaagccatct gcttcattat
    20881 ttttgctata taataatttt tgattcgtat attaagatcc attgacaaac ggtagctttc
    20941 taatgctgcc atcaataaaa acgtacataa aagaaataaa agcaagacag aaaataaaat
    21001 agagccctta taacattttc tactgatcgt tttttgtcca tatggcatat ccatcttctc
    21061 cattttcaaa gtgtacataa aaagaaattg tgtgttcttt ttccaataat tgaagctcag
    21121 tgatacttgt taacataggt tgatgtccgc cggaagttct tcttttacgt atcattgcct
    21181 gatatctttc gatgatatac tgtttatcct cgatcgtgta gtgcagttgc tgatttttca
    21241 cagagacatt gtgactgctc ttgagttctt cttctaattg tgctaaaaat attagccact
    21301 ctttttgatc tttacgctgc aaaaattcac ttacttggat ggtttgctga atcaaaagat
    21361 tgaatgtgag caatagactg ctcaatatca tcagtccaat tagacattca atcagcgtaa
    21421 atgctggaat tacttgtttt ttcaatactg atcgcttctt tctccatatt gacaaacact
    21481 ttttcgatta ctccattatt atcgacaatt tggatatcgt atgggatcat ttcttccctt
    21541 cgctctggtc ttttgtgata catccgctgc gtcttgatat cttcataaat cattcgaata
    21601 aatagcagtt cctccccact tttcttttct gcctgaagca aaaacagctg gccaaagctc
    21661 actaagtaga cacagacaga agcgatcata agcgctgtaa gggactccaa caaaacaaat
    21721 cctgttttat tcaattcttt ttgtatagcg accactccct atctggaatt gatacgtaat
    21781 cgtccgctct ttttcttccc aataaaattg ataagctttt agtgaacttt cattcccggt
    21841 tctagcagca aaagtaatag aagcatggcg tttcaaggta atctcatcag gaatatctaa
    21901 tacttcccaa tttattttat ctggattcgg aacatgaaaa atgatttgat tctcttcact
    21961 attccaataa aagcctgttt ggatctgatt cacaatagcg attttttgcg tagaatagat
    22021 tcgtttttca aattggccga aaaattgata gactgccaat tcattcttcc atgcagaaaa
    22081 tgcgagaact ggaaaagaaa ggatcgttgt aagtaataaa atcagtaaga gtgattcaaa
    22141 caacgtatat cctgatgcta aaaccttacc ttcaaggccg ctgatagatt tctgcatgtt
    22201 ctcgtttgat atagccttca ttcaacaatt cctccacagt aggaactctt ccattatttt
    22261 gaagcgcata gagctctttt tgtgtgtcta ccaactggat gatcgcttca cgactttctt
    22321 ggtctacatg attcctataa cgcgacaaat tagggacgaa caacaacact aatacactga
    22381 tgatcagtaa gacgattagc atttcgatca atgtaaaacc gctataataa gatatttttc
    22441 tctttttcat ggcaaaacct cctctatgct tccgtaaaca ggtaataaaa cagcggcata
    22501 aactaataaa atcaaagccg ctacgcccaa aaaaatgatt ggctgaataa aatgcatcca
    22561 gtgatgaaga cgtttgaaaa gctgttttct tgtccattcg ctgtaaaaaa tcagttcttt
    22621 acctaaactt ccctttgcct ctccctgtaa aacaatttta ctgaattctt ctgatagaaa
    22681 acgataattc tggaactgct cagccaatga catacctgcc tccaatcctt gaatcaactt
    22741 agaggcaaga tgctgaatca gactttcttg tttcgtttcc tttaagcaga ctatgatttg
    22801 ccttaattcg aaaccttcat gaaaaagctt tccaatctct agtgacaaat aagccgattg
    22861 atacaaggaa tatatcggtc caatcaataa ttgtctgctt atccattcac tctgatcaac
    22921 aggagacaac ttcatcagac gataccttac tagtaaagcc aatagagtaa ccgctaggat
    22981 gaatagtaga acataccaat gaaagacttg tatcagctga ataccccaat gctcttgcat
    23041 gaccattcct gaaccaataa gctgaggcaa aacgaacaac cgcatactaa acaagatacc
    23101 tagcaaaaag gtgagcaata gcatcggata tgcaagcagc tttttcaaat cattgcgaaa
    23161 tccttcaaca agccgaaact gtttggcaat ttccaacaaa gttgtcggca gattcccatg
    23221 acgttcagct aactcgatct gtaccagttg ctctgctcca tatcctattt gcgaaagtac
    23281 ctcactaaac gcttgccctt cctttagctg atgttggata tttgccaaaa taacaggtgg
    23341 aaacgaacgg atcgtgagta ataaataaag ggattcttgc agactaaaac cagataaaag
    23401 cagttctccc atggtttgtg caaactggtt ttgctggctt cttgttaatc ctttagaata
    23461 gttctgactg ccaaatattt tcctgagctt cttcataact ttcctcccat gattttttta
    23521 ctgattcttc ctccataaat tgataactcc ataaaacaga aggatggtga ttggcatctt
    23581 ccaaaagttc ttgataaatg acaccagcta aacattcaga tagctcttct tttttctttg
    23641 tcagttcatg gattctgacc aatgtacctt caagattgcg tgcatgtacg gttgcaaaaa
    23701 cccggtgccc tgttagtgct gctcttaccg cagcatgtgc agttgattca tctcttatct
    23761 cgccaatgat tagcaagtct ggacgatgcc gtaatgcgag tttcaaaagt gtatcataag
    23821 tctgtgctat tttcggattg atctgtagct gcagaaaaaa cggttcttct atctctacag
    23881 gatcttcgat agtaatcact tgcaattttt cttccaaagc caaccgatac attagagaag
    23941 tcttgcctga tccgactggt ccgctgaaaa ggtataatcc acgtctgacg gttttctgta
    24001 aaatagttgc aaaatctcga gaactaaagc aacgatgggg tgtttcaaaa gtaccgtata
    24061 atagccgaat cactaaactc tctcgttgaa ggtaatctcc tacagaagac aaccgcagac
    24121 gaacttcctg tttttctaaa gaataagtaa atgcacctaa ctgagctttt cttttttctc
    24181 cgatatccat ctgtcccaaa tacttaaatc ttgagatcag ttgctgtcca tctgccgaag
    24241 agactttctt ccagattgtt cgtttttttc catagcgaaa atagatgtaa aatacttctg
    24301 aagctggcaa tatatatacg tcttgggcat gtttggcaaa actttcactt atcatttgat
    24361 ccgataatcg ttttatttcc attattctcc cctccacaaa aaacatacgt ctttttgatc
    24421 tttttgtttc aaataaaatg aatttacaaa aaaatattgg caaaagccga atttatgtta
    24481 tacttataaa aatttatctt gggaggcact ccattggaaa caacattaag gaaatatatc
    24541 gaagtagcaa gtaaaaaaaa gcctgctgac ctagtgatca agcatgctaa gatcgtcaac
    24601 gtctttacga aagaaatcat ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga
    24661 atcggtgaat acgaaggaaa aagcgtacat aatgctaatg ggcaatatct agtgccagga
    24721 ttcatcgatg gccatgtgca tatcgaaagc tcacttttat caccaagcga attttcaaaa
    24781 gtatccttgc ttcatggagt tactactgta gtgacagatc cccatgaaat aggaaacatt
    24841 gctgggagta caggtcttga gtttatgata gaagatgcaa gacaaacact catgaatatc
    24901 ttttttatgc taccttcttg tgtacctgta accccttttg agaccaatgg tgcaaattta
    24961 aatgcaaaaa aattagcccc ttttttacaa aaacccgaag ttttaggctt agctgaagtc
    25021 atgaactatc aagcagtagc aaataatgaa acagacatcc tagaaaagat ccaattgatg
    25081 caccagcata agaaaaaaat tgacgggcat gcagctggta tcggcatgga agagttaaat
    25141 gtttatcctg cagcaggtat tcggacggat catgaagcta caactgcaaa agaagctaaa
    25201 gaacgtttgg atcttggtat gtatttaatg gttcgagaag ggaccgttgc aaaagatctg
    25261 gcatcgctac taccggcgat cacacctgaa aattcaagaa gatgcttctt tgtcaccgat
    25321 gataaactga tcaatgattt agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct
    25381 attgcacttg gaatcgatcc attacaagcc attcagatgg caacattgaa tgcagcagaa
    25441 tgttttgatt tgaaaacact aggtgcgatt gcccctgggt atcaagctga tttcttctta
    25501 acagacgatc ttaccaagct gccaattcat gaagttttct ataagggaac attagttgtt
    25561 caatctgggc aactccaaga ccatttattt gctgatcatt caaattcata tacgtgttgt
    25621 ctgccaaaac taaacgctca accattaaat ccacattcgt tagagctgct tctttcttct
    25681 tcacaagccc atgtgatcga aattatcccg aatagtctag tgactaaaga tttggtagaa
    25741 aaagtggacc gacaaggcga acgattcatt ccttcaatcg aaaaagacca attaaagatt
    25801 gcggtgatcg agcgtcacca tcaaacggga aacattggat taggcatagt gaaaggattt
    25861 cagctgaaac aaggagcgat agcgacaacg attgcccatg actcgcataa tctagttgtc
    25921 gttgggacaa acgacgagga tatgctgtat gctgctaatc ttttgattca aaaaggtggc
    25981 gggatgaccg ttgtcaacaa acagcaggaa atagcttgtg tttctcttcc tataggcgga
    26041 atcatgacac aggaaccttt ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa
    26101 gcacatcaac taggagcagc taaaaccttt gatccttttt tgacactttc tttcttaaca
    26161 ttatctgtca tacccgaatt gaagatcacc gacaaaggat tattttcatt ttctcgcttc
    26221 caattgattc caccttgtgg aaaaaccgat gaaataaata cattttaaat caaataaatc
    26281 ccagaaaata cattacatct gtaatgttca ttttctgaga tttatttatg gatattaatg
    26341 tttttatgaa agcagaaagt tagtttcaca gacttttctt tttggtctta tcttgaaatt
    26401 gagcttctac tcgataaata cgttgacctt ttgaagaaaa tttttcttca tactccgtca
    26461 taatattgcc ttcaaattga ctttgatgta aatctaacca tacctgtttg aggatcatgc
    26521 catattgaga aaaacttgcc agagaatact caaacaatcc ttgattatct gttttgaaat
    26581 ggatttctcc atttggacga agaatctgtt catctacagc aagaaaagtc ttatatgtca
    26641 atcgccgttt ttcatgacgt ttttttggcc atggatcgga aaaattcaga tagatttgat
    26701 ctacttcgct gtcagcgaaa tactctgtca acgctgatcc atccacatgt agaagtttca
    26761 ggttaggaag cggttgttcg atcaacttgt ctagtgcaat cgaaacaaca ctgacctgca
    26821 tttctactcc aatataattg atttcaggat gggcttttgc cattccagtg atgaactggc
    26881 ctttccccat accaatctca atgtggattg gatgatcgtt ttcaaacaat tcattccatt
    26941 ttcccttcca caatgttgga tcactaatta caaaattcgg gtgagccgct aacatttccg
    27001 ctgctcccgg acgatttcgg acacgcatta tttatctcca ttcctttcct ctaattaaaa
    27061 aggtctggcc aaatctccgc agagtttggg ccaaacctca gccactgaaa attcgtttta
    27121 attgtaaaat ttcttcgttc atccgtcgat gatcttcacg tttgcaatat ctggtgattt
    27181 cttggagcaa attcatggcc gcataccaat gaaatttttc aagcatttcg tcagtcgggc
    27241 gtatgccata actgatcagc cactgattcc agctagacag tggcacataa tttcctaaaa
    27301 tcgttgcaat atccaccgca gggtctgcaa acatgattga atcccaatcg actagatata
    27361 aataattttt acatactaac caattacgat ggtttacatc tccgtgtact actgataagt
    27421 tttccttatc tattgacggg atctgctcac gaagaaaacg atggacaatt tgcagaaatt
    27481 gatttttctg gagttcttcc ggtaattctt tttcatattt ttgcagcatc ttctccggcg
    27541 tcatgacacg accaccgata cgggtcagca tggttttaag agaatttgaa tgatgtaaat
    27601 ggtacaaaac atcaatcacg tcgtttcttt gaccaatctc attaggagtc agcaagcgac
    27661 cttccagcca ctcttgtgct gtcaaagtat cgccattccc cgttcgtttt gtccaaacaa
    27721 gttttggtgt aattccttcc cttgaaagag cagccaacat cggagtcgtg tttcgtttaa
    27781 taaagacttt gtcctcatca cgcatgccta tgtatgtttt tcctgtgtcg cctttgatgg
    27841 gacgtaaccg ccactcttgg tctaactgaa actccatcat gcattctccc gtcctcttac
    27901 tctttgtttt gcagtactta ttattctagt cgcctgaaca ctacacgtca agcacatata
    27961 cgttcttttt catttaattg ccattcaaaa aaccagcttt tctcaaaaaa aatagaacga
    28021 acagcattct tactgtcaac gctctatttt tcattctcat ttttacccta cacagctttg
    28081 ttttcagtct gacatcttct tcaaacgagt tggtacatat agtttaacaa agagcatagt
    28141 aacaatcaaa taagccgcta gcggaaccca tcgctgctgc ccatctaata cgactgcagc
    28201 aatcaaacca aaaataaccg acgcgataat taatacccat gtgagtagat actgaatcgc
    28261 ctgcttcttc tgattcttat ggattggata aagctgaacg agaaccatat agcggaactg
    28321 gttcgctaaa ggtaacagtt ggaaaccgat caaatataaa aataagaaac cgataatcag
    28381 actaaaataa aagtcgacta ctccggcaat caataaccca cctataatta ctaatcggaa
    28441 ataaagacca ctaaaatcag aacctcttgc caatcttctt gcaaacaagt aaagataagt
    28501 gttttttgat tctcttttaa tatgagtcaa tataggatcc aaatagcgtc ttcttttgat
    28561 ctttgccgta atttcaggaa catctgtgaa taaattgata aattgataga tctgatgcaa
    28621 cctatgttct tctacatgca ccattttctc ccaatccaat cctttttctt gttgcttcca
    28681 caaaaaccag taatatagac ttgcctgtac taatgcacct gctattcctg cccatattag
    28741 ccaataaaat gatagaaaca aaaagatcag agaagaaaaa aaccagccta atttccaata
    28801 acgaataact tgttgatcta gtcgaaacaa ttctgctctc ttgatttgca gatgactgaa
    28861 tttcaaactc cataatatga tcagatagac gaaaaacatc gaaaaggatt gctccgtact
    28921 aaccaccact aatggcatgg ccaatccgca tgccaatatc aaaaagatca acggaaaaat
    28981 gcaagaatag cgaaaagctc gttccaaata ggtttgcatt tctttttctt taggaagtaa
    29041 aaaaacaata tctgctgcct ctgcaaaagt agccatctta cccacgaata aacagagcat
    29101 ccaaaataca gagattatga gccctccaat tgcaaacggt ggctgcaggg ttttcaacca
    29161 attagaataa tataatccca caccgccaat caagaaaaga caaactaaaa caaaatgatc
    29221 gttcaataca taacgcatat atttcatcat tttcttttga tgtcttgcta aacgtttttg
    29281 aaaaaaacta ctcatgtgcc tgctcctttg ttaaagcaat atagatttcg tccaaagaag
    29341 aatccggcag ctgaaattct tttctcaaca tctcaagaga accgtctgct cgtatctctc
    29401 cgttatgtaa gaccacaaaa cgatcacaat atttttctgc agtagctaaa atatgcgtag
    29461 acatcaaaat accggctcct tcttttttca tttctaccat caattcaagc aatgcattga
    29521 tagctagtgg atccaagcct aaaaatggtt cgtcgatgat atataaagaa ggctcgatca
    29581 aaaaggcgca taagatcatc actttctgtt tcattccctt tgaaaaatta gctgggaacc
    29641 agtcaagacg attagataac cgaaaagtct ccaacaactt ttctgctctt ttcattgcta
    29701 tatccatagg tatatcgtag gccatcgcag tgacttcgat atgttctctc aatgtcaatt
    29761 cttcatataa agaaggagtc tctggaatat acccaatttg tttgcgatat tcttccgtat
    29821 ttttctttaa ggatagatga ttgatttcta tcgttccttt tgaagcttcc agcagaccga
    29881 taatgtgctt gatggtcgta cttttccctg ctccgttcag tccaatcaac cctactatct
    29941 ctccaggctg tacatcgaaa ctgatatctt ttaacacagg gagatgtccg tacccccctg
    30001 tgacatgatc gataattaaa ctcattcttt ttcctccgcc atttattttt atttattata
    30061 ccacgaagaa aatgacggta ttttgttttt ttccttacaa tagctttaac ttatggtaaa
    30121 gttaagctga agtatttatt ctttatagaa aggatgtttt atatggaaaa ctgtattttt
    30181 tgcaaaatca tcaatcaaga aatcccaagc tataaaatat atgaagacga caaggtctat
    30241 gcttttttag acatctctca agcaacgaaa ggacacacat tagttgtgcc taaacgtcat
    30301 gtagcagata tttttgaata tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct
    30361 aagattgctc aagcattgga aaaagctttt ccagaaatga aaggtcttaa tatcttgaat
    30421 aacaaccgtg aactagccta tcaatctgtt tttcattcac atatccattt agtccctcgc
    30481 tacagcaaag aggatgattt ttctattcac tttgtcaatc atcaagatag ttatggatcg
    30541 gaagaactaa aagctattca agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa
    30601 ctttttcaaa ggactgttat ttggagctgc tgctggcact gtcggaggtc tgttattggc
    30661 gcccaggagc gggaatgaaa caagaaagaa actgatcgaa gaattggaag aagcaacaac
    30721 tctgacaaat gatttaaata acagcctgaa tcattttaaa agcgcattga tcgaaacaaa
    30781 agaaacagct gaatctgtga ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga
    30841 ttttaaattc caagccgaac cacgtatcac acaaatcaat gaacaagtag aaaaactaaa
    30901 agctgatctg ccagagtatc ctgaacaaat ataataatga ttattttctc aacagattta
    30961 actatataag tgagaaaagt ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg
    31021 tttgaaacta ccacgacaac gtttttaaag gaaatgggat atggtatagt taatatataa
    31081 aaataataac taattacagg agtgcaaatt ttaatgaaaa aaaaatcaat tattttggca
    31141 gctactagtg cgttggctgt tttaactcta gcagcttgtt caggcgatac taataaagat
    31201 atcgcaacga tgaaaggcgg aacgatcact gtatctgatt tctatgatga agcaaaactt
    31261 gaatcatcga accaatcctt agtacaacgg atgatcattt ataaagtatt caataacaaa
    31321 tatggcgata aagtaaccga taagcaagtg gatgcagaat acgacaaaca agcgaaatca
    31381 ttaggtgata cttttgaatc acaattagaa gctgctggat atacgaaaga tacttataaa
    31441 gaatatattc gtaacaattt agcttttgaa gctggcttga aagctcacgt agatattaca
    31501 gatgacgatt tgaaaactgc ttggaaatct ttccatccag aagtagaagc acaaatcatc
    31561 aaattatcaa gtgaagatga agcaaaagat gtgaaaaaat cagcggatga tggagatgat
    31621 ttctctaaat tagccaaaga caaatcaact gatacagaga caaaagaaga tggcggtaaa
    31681 gttaaattcg actcaacgac aacaactatt cctgcagaag taaaagaagc agctttcaaa
    31741 ttgaaagacg gtgagatctc cgatgttatt actacaacaa atcctacttc ttatgcgaca
    31801 gaatactacg ttgtcaaaat ggtgaaaaac caaaataaag gcaatgacat ggataaatac
    31861 aaagatcaac taaaagatat cgctactgaa actaaactaa gtgacaatgc attcacaact
    31921 aaagtaatcg gtgaagaatt aaaagatgca aatgtaaaaa tcaaagatga tgcctttgaa
    31981 aatgtgttga gtgcattcac aacaacttca tcaagtacga aagactcaag tgaaactaca
    32041 gcttcaacaa agagttctga cacaaaatca actgattcaa caaaagaatc ttcaactgaa
    32101 gaaacaactg attcatcaaa ataaaagaaa acaaaaaaag cgaagactta tgtctcgctt
    32161 tttttgtttt gaatgaaaaa aataaatttt tgctataaat cttttggtac atagaaactt
    32221 cggttgtcca taccgaaaat ccgatcggtg tattttcctg gttctgtttg acgaatcgaa
    32281 gtcaacatca tctgcatcga agcatcgata ttgtcgattt gatgtaatat ttctgcttcc
    32341 attattcttg gacgtacagg tgagccatat tctaataagc catggtgtgc taaaaccata
    32401 tgacgtaata caagaacatc ttcgtcagct tcgctgattt tcaacgcaag acaggcttta
    32461 gttatttctt cgtcaatcaa taccagatga ccgatcaaat ttcctgcaag cgtatattct
    32521 gtcgacatag gaccggaaag ttcaatcacc tttcctaaat catgcaaaat gataccagca
    32581 tacaacaatg ctcgattcag ctcaggatat tcatcggcga ttgatttccc taagtgaagc
    32641 attgtcaaag tatgataagc taatccactg gcaaatgcat gatggtttct ttttgctgcc
    32701 ggaaactcaa aaaattgttt ttgatattta ttaagtagat agcgaacaat acgattccaa
    32761 tgcgcattgg tgatctcaaa caacaattga ttgatttctt cttccatttc ttctttcttc
    32821 aaaggggctc tttccatgta taaactaggc tctcctggct catctaccct tgttaagcgc
    32881 atatgaagaa tctttacttg agggtttcct tgataaacct ctcttttacc gtttagaaaa
    32941 acgactgtgc cggctgtaaa tttattgatt tcctcctccg aagcatccca gtatttacca
    33001 tcgatagtac ctgaagtatc ttggaaagta aaagcaataa atttttttcc gtttttagcg
    33061 attcttacat ctgcactttt gatcaggaca aattgttcaa actgctcatc gattactaaa
    33121 tcacgaattt ttttcattat ttgctccttc taattgtttt acgatttggt tcatctcatg
    33181 gtaatagctt accatttctt tatcagaaga aaaacaaatt atttgatact tttctccaaa
    33241 tttagctaaa agttcagcta attgatactt tcgctgatga tcataatgaa gccagccatc
    33301 atcaatgatg atcgggcata tcgaacggtt agcttctaat gagaggtaag caaatctcat
    33361 cgccataatc agctgatctt tcgtacctgt agataactca tagatgggaa attctgtata
    33421 tccgtctgtt aaagtcagtg tatcattttt cagtaatatc ttctgataat ttccttttgt
    33481 taaaatacct aaataatcac ctgctgctct taacaactgg ggtaattgtc tttcactcat
    33541 ctctgttgaa agatcaccta agaatgtccc ggcgatttca agtgctcccc attcctccag
    33601 taattcctgt aattttgctt tttgccggct agcttgttga taaagttcgt ccaatgtgcc
    33661 gtctctttgc aactcttcta cttgaagttg gagtcgttgc cgattttcac ttgcttcttt
    33721 ttcttcttgc tggagttggg attgcttttc tttcagttgg cgcagtctta cagataattt
    33781 ctcttttgtc agctcttcag gaaatagtgg agtaagcatt tccgataatt cattgtatct
    33841 tttcatctgc tgttcggctt gtttggcttt ttgtaaaaag acaggtactt ccgaaggata
    33901 agataagcct gcttgagtca attgttcgct gtattcttgt atccatactt tttgtttctt
    33961 tttcaattga ttgatttctt gcttcaataa agtattttgc tgatagcttc tggcaaattt
    34021 catctgttcc atctcttgta taaaacggga aagcaatacc attttttctg aaattgtctt
    34081 atcttgtatc ggtaaccatt ctttaacaaa ctcaaatttt tggtcgattt gtcgtagttt
    34141 ttcttgtatt tcctgttctc ttttatgttt ccgctcaaat acgccatata atttctgata
    34201 atctatgact tcctgtccat attctttcat gagtttgagc gtatccatct ttcctaagtg
    34261 ccgatgatcc gcaaaacgat atacttcttg ctgaagaatc tgtttttctt ctccagttct
    34321 cttttgtttt tcttctgctt cagctaattg agctaaggct agatcaagtt gtcctagttt
    34381 ttcttgccac attcctttta tctcagagaa atcattttta gttttaattt ttggttgcct
    34441 tttgtagaag aaatagccaa tcatgcctgc accaattagt aaaaggatca ctttcaaggg
    34501 cattggtaca aatatacctg caatgattat tgctccgcca gccatcagca tatacggttt
    34561 agatgaagag actggtgcta tctctgactg attttgtaat aatgctggat aggtctcttc
    34621 caagtcattc acttctgctt caatcatctc ttgctgttct ttagctattt ggagacgcac
    34681 agaaatctgg ctttctagat cagcaatcct tctttcttca gccagaagat gcttccattc
    34741 ttcttcatat aaaaattctt ccggtaacgg atttttctgc caatgccacc tttgttccaa
    34801 aatcttcaat tctgaatttg tttctctagt ctcaatttgt aatctttgcc attcttcgtt
    34861 taattgttcc gctgtaaatt gaagttgctg gagatctttt agttcctgct cctgctctaa
    34921 ataaaaataa tatctggctg actgctgatc catcccgcta tgcttttcca gtgttgcttc
    34981 taattcttcc aaacgctcat ttgtttgctg ataccgtttg gagaaagact ccagatctaa
    35041 aagaagttgc tcatcaggaa gacctgtagt gctgtttttt ttgagagatt gccattcttc
    35101 atataaagaa aaattaagtt cttgctgtct ggctaatgaa aattgtttgt tcgcctcttt
    35161 taattgtgcc aataactgct gctgttgcga ctcgtattcc tgttcttgtt ggatcaactt
    35221 gccaaaataa gcttcctgtt cttgtttttg atgaattttt tcttgcagct ctttccattc
    35281 atttaatttt tgattgatta gtaatttttg gccttttttc ttgaataatt gttgtgcttt
    35341 ctgataatat tcttgccgct tttggaatac ttgagaactg cccgtgatgc ctaaagaaat
    35401 cagcgcatca tgtagctctt tttctctcaa atgatctagt tccatcaact gctcttgctg
    35461 gaaagtaaat acctgctgga ataactcttt cgtcaaaggt gcgatcagtt tttctagtaa
    35521 ctcatcactt cccacttgat cccccagcca caccttcgat tttccgcgat tttgctgctt
    35581 gtagcgctcg acagcaaatt caccatatac aggatgttta agccatattt tgccgccata
    35641 ggctgagccg tcttttggtg tataatcctt ttttttacgg ccttttgaag ggaaaccaaa
    35701 taatattgct tgaataaatt gataaatcgt tgattttcct gcttcgtttc ccccatagat
    35761 cagctgattg cctgaataaa agtcaaaact tttttgacgc cattttccaa aagcagcaat
    35821 ctcgatcctt aaaatcttca ttcctgatcc tcctcaaata aaaaatcttg attaagcagc
    35881 tgcttggctt tttcaagtgt ttcttcttga tattccggca gttcgctcaa tattctagcc
    35941 gcttcttgat gactatatac ttcatttaaa atttgttgga aatcttgagg tgtctgatag
    36001 tattgaaata actgttccat caatttagca gaagcagcaa gtggggtctt cgtataaaaa
    36061 gtattctcta tgagagaaat acgccaaata aagaaatcat ttatcctatc tgagaattcc
    36121 tctaacaaat agtctaatag ctctcctgat tgtatccgtt ccaaaacatc tgtattgaga
    36181 tggtcataat cttttaattt tatctcaatt aaactaaatc catcctctac ttttgaaagc
    36241 tgctgacgaa tgtgaacaat gacctctttt gtcgttcttg ctttcctcaa tgatatttcc
    36301 tttgtcttcc aatagacctc tgctacttta atcggatgta tttggcaact tcctttactt
    36361 aattcaacta gtagaataga tgtagaagct tgttcctttt tagtatgacc ttgaggcgca
    36421 cctggataaa caattgtttg cttttcattt aagaccgttg gcacatggat atgccctaaa
    36481 gcccaataat cgtatccctt ttcttgcatc tttgagggtt ggaaaggagc atagtttcct
    36541 ttttgagaga cgccaggttc cccgtggtac aacccaatat ggtaatctgt gagttctcta
    36601 gaaggaaatt ccatcacttt gtctttttga atccacggtt gacgataact aaatccactg
    36661 agagagacag tttctccact ttttatcgtt attttttttg tctctaccgt ttcggatgta
    36721 aataaatgga catttttagg aaaggagaac caataccgct ctttttgata gaaatcatga
    36781 tttccgaata ttataaatac aggtatatgg ttcttttcta accgtttcat ctgttctgaa
    36841 aaatgtttct gtatttttaa tgaaggacga ttttgatgaa aattatctcc tgcaagtaaa
    36901 acaaaatcaa cgttatttat tattgcctgt tcaatgatat ttgataaaac ggtcagattc
    36961 gtcttcaata gtttttcctg tacagtttta tcaagagttg tcagtccttc aaatgaccga
    37021 tccatatgta aatctgccgc atggataaat ttcaccatta aaaatttcct cctttttttc
    37081 ttttatcata ccattttcaa tgtttttttt catcaataac cgaaaatttg ttcgt
//
LOCUS       DAZUKD010000015        36981 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000015 DAZUKD010000000
VERSION     DAZUKD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.015"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..543
                     /locus_tag="ACG8N9_000617"
     CDS             130..543
                     /locus_tag="ACG8N9_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4896244.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            680..940
                     /locus_tag="ACG8N9_000618"
     CDS             680..940
                     /locus_tag="ACG8N9_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896245.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            1058..1336
                     /locus_tag="ACG8N9_000619"
     CDS             1058..1336
                     /locus_tag="ACG8N9_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896246.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFF"
     gene            complement(1515..2708)
                     /locus_tag="ACG8N9_000620"
     CDS             complement(1515..2708)
                     /locus_tag="ACG8N9_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4896247.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2792..2992)
                     /locus_tag="ACG8N9_000621"
     CDS             complement(2792..2992)
                     /locus_tag="ACG8N9_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4896248.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3416..3649)
                     /locus_tag="ACG8N9_000622"
     CDS             complement(3416..3649)
                     /locus_tag="ACG8N9_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896249.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3656..4087)
                     /locus_tag="ACG8N9_000623"
     CDS             complement(3656..4087)
                     /locus_tag="ACG8N9_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4896250.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            4533..5216
                     /locus_tag="ACG8N9_000624"
     CDS             4533..5216
                     /locus_tag="ACG8N9_000624"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4896251.1"
                     /translation="MSLKGKIPPEVKIQAVEDYLAIRKGSTQIREELGIRLSTFQAWL
                     RKYQTEGPAGLHPKKHVTSYPSSLKLAAVEDYMGGGGSLDAMCRKYGISSHAVLQQWI
                     TLYNQGHREFKTRRAREETDMAEKEKLSQEQKLQAVLYCLEHQLDYRRTSEQYKITYQ
                     QIYSWVKKYQEQGEAGLLDRRGRRRPPSEYSEEEKAAAKLRQLEAENRRLQMENDFLK
                     KLNELEGRR"
     gene            5189..6103
                     /locus_tag="ACG8N9_000625"
     CDS             5189..6103
                     /locus_tag="ACG8N9_000625"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4896252.1"
                     /translation="MERTGGEAVNRLTRRRKVYEAIHTLRQEKGYEIASLCALSGIPR
                     CSYYKWLHREESASEKENTLLLEEIIRLYSEVKGIYGYRRMTLNVNSRLKRNYNHKRI
                     YRLMKSVHMQAVIRRKKKHYVILEPRVTAENVLNREFTANGPNEKWLTDVTEFKLING
                     KKAYLSAILDLHDKSIVAYALGQSNNNQLVFDTFDRAVRANPGAHPLFHSDRGYQYTN
                     RQFKAKLDGIHATQSMSRPGRCIDNGPMEGFWGILKSEMYYLRQFHTFEELKKAIDEY
                     IDFYNTRRLQAKLKGLAPLAYRNQTLVA"
     gene            complement(6355..6963)
                     /locus_tag="ACG8N9_000626"
     CDS             complement(6355..6963)
                     /locus_tag="ACG8N9_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4896253.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(6969..7997)
                     /gene="vanB"
                     /locus_tag="ACG8N9_000627"
     CDS             complement(6969..7997)
                     /gene="vanB"
                     /locus_tag="ACG8N9_000627"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4896254.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(7990..8961)
                     /locus_tag="ACG8N9_000628"
     CDS             complement(7990..8961)
                     /locus_tag="ACG8N9_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4896255.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(8958..9785)
                     /gene="vanW-B"
                     /locus_tag="ACG8N9_000629"
     CDS             complement(8958..9785)
                     /gene="vanW-B"
                     /locus_tag="ACG8N9_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4896256.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(9803..10609)
                     /locus_tag="ACG8N9_000630"
     CDS             complement(9803..10609)
                     /locus_tag="ACG8N9_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS4896257.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(10780..12123)
                     /gene="vanS-B"
                     /locus_tag="ACG8N9_000631"
     CDS             complement(10780..12123)
                     /gene="vanS-B"
                     /locus_tag="ACG8N9_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4896258.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(12126..12793)
                     /gene="vanR-B"
                     /locus_tag="ACG8N9_000632"
                     /pseudo
     CDS             complement(12126..12793)
                     /gene="vanR-B"
                     /locus_tag="ACG8N9_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
     gene            complement(13006..13179)
                     /locus_tag="ACG8N9_000633"
     CDS             complement(13006..13179)
                     /locus_tag="ACG8N9_000633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896259.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            complement(13411..13578)
                     /locus_tag="ACG8N9_000634"
     CDS             complement(13411..13578)
                     /locus_tag="ACG8N9_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896260.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(13736..13921)
                     /locus_tag="ACG8N9_000635"
     CDS             complement(13736..13921)
                     /locus_tag="ACG8N9_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4896261.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            14066..14437
                     /locus_tag="ACG8N9_000636"
     CDS             14066..14437
                     /locus_tag="ACG8N9_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4896262.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            14695..15024
                     /gene="mobC"
                     /locus_tag="ACG8N9_000637"
     CDS             14695..15024
                     /gene="mobC"
                     /locus_tag="ACG8N9_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4896263.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            14985..16313
                     /locus_tag="ACG8N9_000638"
     CDS             14985..16313
                     /locus_tag="ACG8N9_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4896264.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            16310..16681
                     /locus_tag="ACG8N9_000639"
     CDS             16310..16681
                     /locus_tag="ACG8N9_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4896265.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            16864..17100
                     /locus_tag="ACG8N9_000640"
     CDS             16864..17100
                     /locus_tag="ACG8N9_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896266.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(17400..17603)
                     /locus_tag="ACG8N9_000641"
     CDS             complement(17400..17603)
                     /locus_tag="ACG8N9_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4896267.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(17607..18551)
                     /locus_tag="ACG8N9_000642"
     CDS             complement(17607..18551)
                     /locus_tag="ACG8N9_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896268.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(18552..22472)
                     /locus_tag="ACG8N9_000643"
     CDS             complement(18552..22472)
                     /locus_tag="ACG8N9_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4896269.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLPNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(22621..24702)
                     /locus_tag="ACG8N9_000644"
     CDS             complement(22621..24702)
                     /locus_tag="ACG8N9_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4896270.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(24699..25928)
                     /locus_tag="ACG8N9_000645"
     CDS             complement(24699..25928)
                     /locus_tag="ACG8N9_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS4896271.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(25918..26169)
                     /locus_tag="ACG8N9_000646"
     CDS             complement(25918..26169)
                     /locus_tag="ACG8N9_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS4896272.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(26192..28183)
                     /locus_tag="ACG8N9_000647"
     CDS             complement(26192..28183)
                     /locus_tag="ACG8N9_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4896273.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(28215..30614)
                     /locus_tag="ACG8N9_000648"
     CDS             complement(28215..30614)
                     /locus_tag="ACG8N9_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS4896274.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(30544..30966)
                     /locus_tag="ACG8N9_000649"
     CDS             complement(30544..30966)
                     /locus_tag="ACG8N9_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS4896275.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(30980..31516)
                     /locus_tag="ACG8N9_000650"
     CDS             complement(30980..31516)
                     /locus_tag="ACG8N9_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS4896276.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(31553..32416)
                     /locus_tag="ACG8N9_000651"
     CDS             complement(31553..32416)
                     /locus_tag="ACG8N9_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS4896277.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(32508..32723)
                     /locus_tag="ACG8N9_000652"
     CDS             complement(32508..32723)
                     /locus_tag="ACG8N9_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS4896278.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(32800..34608)
                     /locus_tag="ACG8N9_000653"
     CDS             complement(32800..34608)
                     /locus_tag="ACG8N9_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4896279.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(34605..35081)
                     /locus_tag="ACG8N9_000654"
     CDS             complement(34605..35081)
                     /locus_tag="ACG8N9_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS4896280.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(35156..35368)
                     /locus_tag="ACG8N9_000655"
     CDS             complement(35156..35368)
                     /locus_tag="ACG8N9_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4896281.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(35390..36355)
                     /locus_tag="ACG8N9_000656"
     CDS             complement(35390..36355)
                     /locus_tag="ACG8N9_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS4896282.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
BASE COUNT         8340 a         9814 c         9344 g         9483 t
ORIGIN      
        1 aagatagcct ttgtatcgta ttagatgaaa tattcacaaa gctaaccatt atacttgcat
       61 ttttttcaaa atagagttat tacgtaaata gggataaata aaaaatactt attgaaagga
      121 tgatcaaaaa tggcaaatgt acctagcatt cgcgacatgt tccctgattt caatgacgtg
      181 tttagtccag cattcaatga ttttttaggt gtttcaagtt atccaaaagt agatttagtc
      241 gaaaatgaaa aagaatacaa actaactgct gatatgcctg gctgtgacaa agaagacacg
      301 acagttgaat attcagataa tactttgacg atctccgcca atcatgagtc ccatacagaa
      361 gataaagaag atggcaacta tgtacggaaa gaaagacatt cggtctctta caaacgttca
      421 ttctatcttc ctaatgttga tgaagaaaaa atcactggaa cctttaaaaa cggtgtgctg
      481 aaattggtat tacctaaaac agcttatcag ccaaaagaaa caaaaaaaat tgagttaaat
      541 tagaacttat cgcctctcga tgaatgaggg gcgattttta tatatttgga taagaacgag
      601 tatgtttaaa ttttcaaaat gagcaaaaaa atattttgtt tttacgataa agatgttatg
      661 atctctcttg gtaaaaaata tgggagttga tcatttgatt gtcttaagta aacttttgtt
      721 ttggatacca tttatcataa tgaccagtgc tttcttttac ttattcaaaa ggcctaacaa
      781 atacttactc ttatctgctt gtccgatgat ctattttatc cataagatta gaggatttta
      841 ttattacgac tctggtatct cattgttttg ctacgaaatc ggcttattag cttcggccat
      901 ctttttcata tttgtgttag aatatttcag aaaaaaataa acttccagtc agttttggct
      961 ggaagttttt tatttggtgc agatcttttc agctgttatt catacaagtt gatccatttt
     1021 tctattatta agtacatacc attgttactc tttgaatgtg ggggagaaaa aaataaaagg
     1081 agctggtatt ttgttagtta tgagtaaagc tttgttttta cttccctgca cgttcatgtt
     1141 tattgtttta tgttatttca aaagaagaaa aaaatggtgc tggatcgatg ttctactatt
     1201 ccttcccttt ttcccgctta tttattttct tgaagaaata tgggatgata tggcatgtga
     1261 ttctgctttt ctttttagtc attcattggg gcttatgatc tctcttacga tctttgccat
     1321 tgtgctattt ttttaggagg aaaattatat gggattttag attatctttt tagaagttct
     1381 tgaatgtcgg tatagcccgt gtttccgggc ttttccggca tttttgatat tgttagatac
     1441 ttacaaatgt tcttattacc tctcaccatt tccccctcaa aagtagtaaa tggagtagta
     1501 aaaccggcct tgtgtcaggc tgccagccgt tccatttctg cccttgcgga atcgaatgtg
     1561 gcgtgtgcat aatagttcag cgtcatggtg atattggaat gtcccataat gtactgcaag
     1621 gctttggggt tcattcccgc attggctaac cgggtgcaga atgtatgacg catagcatgg
     1681 ggcgtcgtcg ttttcggcaa cgcttcttca tggctcttgt tgtacttctt cacaagcctg
     1741 ccaaacatcc cgccataatc taccgccgtc atgggatagc cgttctgttt gagaaacagg
     1801 aaattggcat acccgtcaat aatgaacggc tttgcacctt tgcggttttt tatcactcgc
     1861 tggaacgcct gatacacttc ctcattcatg gggatttttc tgacgccgct ttgggtcttg
     1921 ggttcgtcaa tatagtaacc gacgccggta tttctcaaaa gctggtggct cacgatgatt
     1981 acccggtttt caaaatccaa atctttgtcc gtcagtccgc acagttccga aatgcgaagc
     2041 cctgtcccca gcagaataac gattgcgtca taatgctttt ggtaaacctt atcaccttga
     2101 ataaaggaca gaaaactttc ttcctgtgct ggtgtaagag gcacctttgg ttccgtatcg
     2161 tcgtccagaa cgtcactaag ctggaagtca aacgggtttt ttcggatgca atcgtcctgt
     2221 atggcggtgt aaaaagccgc tttcagggaa cgcttgtcgt tattgatggt cttataagaa
     2281 aggccctttt ctttcatccg aagcgcccat tcctttgcgt cggacagctt cacactgtca
     2341 atcgggcagc tccccagccg atcttgttcc agaatacgca tcagccgttc ccggcccagt
     2401 ttggtgttgt gcttcacatt gccccggtgc ctgatctgct tggcgtagag ctggcagacg
     2461 gtcattttct tgccgatggt gtctatcccg tcgtcaaggt ctttctggat ttctttctcc
     2521 ttttcccgga gtgaaatatc ctcccgttta cctgccgggg tcttatccgt aggcactaac
     2581 ttccatgagt aaacaaactg cggttttcca aaggtatcaa catatttgta agcatatcgt
     2641 ccgtcttttc tctggctctc tccagaccgc aaaacgcgat ttttgctgtc tcgtcttttt
     2701 tctgacatct cgtcatgctc ctttccatga cggaaagagc cgcatatatg cttataaatt
     2761 attataacat atctgcggcc ctttttcact attagattgt gtccaaagtg tcaattattt
     2821 tctcaaactg cttgcgtttg atctggatgc ggttgccatt cataatgacc cagccagagg
     2881 ccggattttc ctcggccagc ttacgcaatt tgttttctcc gatacggaaa tatttagacg
     2941 cttcctcaat cgtcagcgtg tatttttccc aaataggcac atcatggttc ataatgcagc
     3001 cccctttcac aaattcaaag gtttgtgtga tttaggggca tagacggacg gctgctggca
     3061 cagctccacg ggactttcac ccccgcgtgg ttctcacgcg gccctaccca ttgcctgcga
     3121 cgcttttaac gctcggactg tggctataag ggagtatcat agtcctgcct gtacgtcgtc
     3181 gcccgcaagc cgccacgctc acattgcggc gtggtgttcc tcgctccgct tttcccgtag
     3241 ggaaaagtat catggcgcac cagccgtccg gctcggcaca gacagaatgt gtccgtatgc
     3301 cctatgatgc gccgccgtta tcttgcgggc atatttatca aggtgcgatc aggccaccgg
     3361 gaaaacaccg aaacggggaa agggtaagcc gtttcatgct gccggggccg gttcgtcagt
     3421 tcgtgacgat agcgtggatg agcttgattt ccagacggcg cttcatggcc tcgtccagac
     3481 acacatgcgg atagccgttt tcgtcataga gcgtccgggt acacagcttg ttcatgtaac
     3541 cgctgtaata ctgcaaaacg cgctctacgg ctccggcatc cccagcgcgg gcggcctcga
     3601 tcaccgaaag gggcaaaagc tcaatgccct tgtagttcgg gttcttcatc cgcgtctaac
     3661 ctcccttcgg catcaacgcc tttagctgtt tccgcagctc cgataaggta tttgtcctgt
     3721 gccgctgaac ggcactccgg gacattccca caaggccgcc gatctcacca tctgccatat
     3781 ccagaacaca gtgcaggatt aaaatactct gctccatgct gggcagcgcg gcaaaggctt
     3841 cggcaacaag ctcattgttg atatgtaggt catagccatg cgacgaaaac gtaaagctgt
     3901 cgctggggta gtgatctact gtacagagct tgtccaaatc tgcctgtgac aaagagctta
     3961 gtgacacttc acggtcacgg cggcgcttca tttcagccaa gtagcttttg gcctcgttac
     4021 gcagtaccgt cttgcaaaaa gcatcgaaca tgcaccgcgc ttcatagtcg cgggggatag
     4081 catccatctt ctcaccccct ttctgtgggg gacttaggtg gttctctccc tttccgctaa
     4141 caagactttg acaaggccgc cagctacccg ctaaatacct cctttttgaa aatttctcaa
     4201 aaaatttttt tggggacagc cgggccgcaa aagacgacaa aaatcgcccg gcaggtcgca
     4261 aacctaccgg gcgagaaaat gctttgctat gctgctgatt tacatggtat agtacatact
     4321 cataaccgga atagccctgt ggaggggcaa ggcttttttt gagtggtgga agtggattag
     4381 agcatggcaa tagcaaaaca gcatagcggc atcctcctat gggccgccac accttttgct
     4441 gccagaaaag aacggcggcc ttgattttga tatgctccct ctgaagtaga cagcaaaaaa
     4501 attaaactgt cgaaacggag ggagcaatta tcatgagttt aaaaggcaaa ataccgccgg
     4561 aggtaaagat tcaggctgtg gaggactacc tagcgatccg taagggttcc acacagatcc
     4621 gggaagagtt aggcatacga ttgagcactt tccaggcatg gctgcggaaa tatcagacgg
     4681 aggggcctgc gggtctgcat ccgaaaaagc atgttacaag ttatccatcc tcactgaagc
     4741 tggcggcagt agaagactac atgggcggag gaggctcgct ggatgccatg tgccgcaaat
     4801 acgggatttc ctcccatgcg gttttacaac aatggattac gttgtataat caaggccata
     4861 gagagttcaa gacacgcaga gcgcgggagg aaacagatat ggccgagaaa gaaaaattat
     4921 cgcaggagca aaagctgcag gcagtactat actgcttaga acatcaactg gactaccggc
     4981 ggaccagcga acagtacaag atcacctatc agcagatcta tagctgggtc aaaaaatatc
     5041 aggagcaggg agaagctggg ttgcttgacc gccgggggag acgccgccct ccttccgaat
     5101 acagcgagga ggaaaaagcc gcggcaaagc tgcggcagct ggaagcggaa aacagaaggc
     5161 ttcaaatgga gaacgatttc ttaaaaaaat tgaacgaact ggaggggagg cggtaaacag
     5221 gctcacacgc cgcaggaagg tctatgaggc gatccatacg cttcgtcagg aaaaaggata
     5281 tgaaatcgca tcgttatgcg cgttatccgg gattccccgc tgttcttact acaaatggct
     5341 tcaccgggaa gaaagtgcct cagaaaaaga aaataccctc ttgctggaag aaatcatccg
     5401 tttgtacagc gaagtaaaag gcatctacgg ttatcgccgt atgacgctga atgtgaacag
     5461 ccggctcaaa agaaactaca accacaagcg gatttaccgc ctgatgaaaa gtgtacatat
     5521 gcaggccgtg atacgccgaa agaaaaagca ctatgtcata ttagaaccgc gtgttaccgc
     5581 ggaaaacgta ttgaacagag aatttacggc aaatggtccc aatgagaaat ggctgacaga
     5641 tgtcacagaa ttcaaactta tcaatgggaa gaaagcatac ctgagtgcga tccttgatct
     5701 ccatgacaaa agcatcgttg cgtatgcact gggccaatcc aacaacaatc agcttgtttt
     5761 tgacaccttt gacagagcgg tgagagccaa ccccggcgcc catcctctgt ttcacagcga
     5821 caggggctat cagtacacca accggcagtt caaagcaaag ctggacggta tccatgcaac
     5881 gcagagtatg tcccggcccg gccgctgcat cgacaacggc cctatggagg gcttctgggg
     5941 gattctcaaa tctgaaatgt actacctccg gcaatttcat accttcgagg aattgaagaa
     6001 agccatcgac gaatacatag acttttacaa tacaaggcgg cttcaagcta agttaaaggg
     6061 cctggctccc cttgcttaca ggaaccagac cctcgtggcg taatttttct tttttcatct
     6121 gtctacttga cagggggcgg ttcatttctc aaaaccgccg ccagcaaaat acaaaatggg
     6181 cgtgggaaag gtctgcccaa cagcggtttt tttcttttct gccgctgggc agattgaata
     6241 ctataatttg caatgtatta caaaagatgc tttaaccact ttccagtaaa agattgtcca
     6301 tgaccagcat agattttatc aaatccaaaa tctgatacaa aaccaatact ttcattatga
     6361 aacggcaaaa ttaaaatagg tatcggggta tggctcatca atcaacttat agtgccacca
     6421 ttcaaagcga taagactgaa atccgctgct ttccatgatt ttacgcaagc atcttcgatt
     6481 ttgtgcctct gcatccccta tcccttttgc tgtatgatgc gaccgttcgt ccataaaatc
     6541 gaagttactt cccattgaaa caagttcccc tgtatccagg tggtagagcg taagatcaat
     6601 tgcgcttcca cggctatggc tggattgtga ggccacataa ccctttgtaa tcaactcggc
     6661 tcgctctata ttggggtaaa atttttcttt tgtgagattg tcctccggtt gcgctgccca
     6721 acgcaggaag cagtccaccg cagttcttgg ccgatatcca tcccataaaa gcaagccata
     6781 gccaagcgca gccgcatgga tttgtgcctc gcgcagagca aacgccacgg cctttgtgcc
     6841 gatgatgcga ttcacctcat acccatccac cggttttcct gtgaagttat cccatgtggc
     6901 gtacttggca tcccaacgga cgccatgcaa catttcatct aaaaacaaaa aaccattttc
     6961 catacttatc acctctttaa cgccaatgta atcaggctgt caatcagtgc aggaagcgtg
     7021 attcctgcgg cggccatcat acgtgggtag cggctgtacg atgtaaaacc gggcagggta
     7081 ttgacctcat ttagaacgat gccgccatcc tcctgcaaaa aaagatcaac acgggcaagc
     7141 cctctgcatc caagcacccg atatactttc tttgccgttt cctgcacccg atttcgttcc
     7201 tcgaccggaa tgtctgcggg aactgtaatc atcgcatttt ctgagccttt ttccggctcg
     7261 ttttcctgat ggatgcggaa gataccgtgg ctcagccgga tttgatccac ttcgccgaca
     7321 atcaaatcat cctcgttccc catgaccgca cacccgacct cacagcccga aatcgcttgc
     7381 tcaattaaga tttttccatc atattgtcct gccgcttcta tcgcagcgtt aagttcttcc
     7441 gtaccgttta ctttggttac gccaaaggac gaacctgacc gtgccggctt cacaaagaca
     7501 gggtaggtaa gcgcacccgc ctccggcttg tcacctttat caatcatttg aaattcggga
     7561 acggcgatgc ccgcattttt tgtaagaatg taggccagtg atttgtccat gcaagctgcg
     7621 gagctttgaa tatcacagcc cacatagggg ataccagaca atacaaacag cccctgtatc
     7681 gcaccatcct ccccgcattt gccatgcaaa accgggaaag ccacatcaat acgccgtgtt
     7741 tcgtattcgc tttctttcat gacaagcagc ccatgcgttt tcctatccgg ggagagtatg
     7801 gcggggagac tgtcggcttc ccattccgta catggcttct tgcatagctt ccatacaccg
     7861 ttttttgtaa ttccgatgta gtgcggatcg aatttttccg tatcaatgtt cgcagcaatt
     7921 tctattgcgg attttaccga cacatcatgt tcctccgagc aaccgccgaa gatgattgcg
     7981 acttttattc tattcatgct gtaaactcct ttcaaaattg agacaattcc tgattgtttt
     8041 ttctgtagta tcctgcagca cacgctcagt gtagtacgcc gtatggggtg tgatgatcac
     8101 attcggcatc cttaaaagct gcgacaaaaa gggatggtca agcactttct gcgagcaatc
     8161 ggtataaaca aactgatcct cgccctccaa cacatccagt gccgcaccac ccagctttcc
     8221 gctttccaac gcctccacca gcgccccggt atcgacaagc gctccgcgcc cggtgttgat
     8281 taaaaatgcg ccttgcttca tctctccaat ttgcttctga ccgatcagat ggcgggtatc
     8341 cgcacaaagc ggcacatgga gcgtaacaat atcgctgttt tttagaagct catcaagctg
     8401 gacataatct gcatccattt ttcggctgtt atcataggcc agcacatggc atccaaatcc
     8461 ccgcagcctt ttgacgaccg cttgcccgat atggccggtt ccaataactc ccacagtcat
     8521 atcccccagt tctttccccc ggatacgatc caatctgaaa tcttgttgcg ccacggcgtg
     8581 catggtgggt tttgcacccc gtatggccat cagcatcagc atcaaagcat aatccgcaac
     8641 gctgtccggc gaatacgcaa ccgtgccaac cgagattccc attctctcgg cggcagtcgt
     8701 atcaatgtga tcgcagccga tgctgcgggt agaaatgtat tttaccccga cctttctcag
     8761 cgcaagaatt gtcgcctcgg aaacctcgga cttatggcct acgctaacgc attgattgcc
     8821 agcggccaat tttgcgttgt ctgccgatat cgcatcgcta atcagcgtag ggataatatg
     8881 aaaatccggc gataaagcgc ggaaagcatt tgcctcatcc tgttcgcatc caaaaacagt
     8941 aatgcccaca ctttttctca ttggttctcc tcctgaatat ccacactttc cgggagggga
     9001 taacagattt tgcatttgtt tgtataaagc agcttgcaat ctattatttc cccggtacct
     9061 aaggcaactt gcatccgttt aacctgggca tattcataaa tcccgccact ttcacggaca
     9121 tactggatac tgccatttgt aattttgtat aatgcttggg gctctttgtc ggcggacacc
     9181 tgaccgatga ttttctcatc atctaaggtc acccatattt ggtaggtgca gtcggtatcg
     9241 tttcgcactt ttaaatcaat ccagccctct gaaatggttg catccacccc tttaatctcg
     9301 tcactgtttg gctctggaaa ctcctttact tcgtgacctc tgcgctggat aattgtcaat
     9361 ggcgtatgca ggaacatcca aaatagtaaa ttgctcatct ggcacatacc gccgcccgac
     9421 atggtggtga gtttaccatt ggtcaccgta aggccgtctt tatagggggt atctttgtcc
     9481 gcatggcgta ccagccacca gaaagaaaag gtttcccccg gtcttatcaa taagccgttt
     9541 aaggtctttg ctgccagctt taagttgaac accttgtttt cctggtatat catgtcaaat
     9601 ccggtattgt ggttgtataa tgcgcaatcc gtttcaaaga gcaaatggga aagcgatttt
     9661 tcccctatcg tctgcgcata gcgacagccg tcaaatctca ttcccgcata aaagcacatt
     9721 tttctctgcg cccgtctcat tggaagcagg aacgggaaac gctgtgtcaa tctttttctg
     9781 tccataacat aacatccttt tctcattttt ctggttttaa atattcctca aggcaaaccc
     9841 cttggttgta catctctgtg gcagcttcga tgccaacata tcggtaatgc cacggctcgt
     9901 tgctcacacc ggttatctct gtcttgtctg gtgggtagcg acgaataaaa ccaaaacgat
     9961 agctgttttc atccagccat ctataaacct cgttgcctgt tgaatgaatt ccgtccgcat
    10021 tgatatccac agcaagacca agctgatgct cgctcgttcc cggcacggcc acccaagttt
    10081 ctgcttccgc tttagcctgt gcagaggtgt agcctttcgc cttatattcg gcaatttttt
    10141 catccataat ttcttgctgt ttttctgttg ttcggtatcc ggatgcgaca atcgggtaaa
    10201 ctccatcagt tcttgcggca tcaaacaaat cttgaagata gggagaaatc cgaatatcta
    10261 tccgctcacc atttgatagt tgctcaagct ccacatcgta ctgtgcgggg atggggttct
    10321 gcctgttcac taaaatcagg ctccattcct gttcatctcc ctgttctttg gcggtggcaa
    10381 gagcgttttc ctccaaattc tccactacgt cagtgctgct gcctgtatga gttgcaggga
    10441 tgggcggtat ctgtgttgcc tccaatacgg aagccagtct ccaccccaaa aacagcgtgc
    10501 agactgtgaa cgaaataatg aacgcacata gaaaagcccg cttttccacg gattgtttca
    10561 tatgccgttt gtgatgatcc gcattggaat gatagttgct tttttccatt tgatcgaatg
    10621 cctcctttgc aataaaacta cgatttgtgg ctggtacgat tgttattatg gcaagcgcat
    10681 ctgtactttc cctgtagcca gtctgtagtt ttcctgtagc agcaaaaaaa gaccggcagg
    10741 gttatacctg tcggtcaaaa tcatttaagt tttaaatatt tacaatgttg atgtgagcgg
    10801 taaatccagc cagaacaaaa cgccatccga ggtgttttcc agcgcatact ggaggctcat
    10861 ggcatccagc gttttttgta cgatggcaag tcctaaaccg cttcgcccac tttttctgct
    10921 tcgcgcctga tcaatgcgat agaatggggt gaacagcctt ggaagcgcag tatcatcaat
    10981 gtgaacgccc atgttcaaaa caaaaaggcg gcatttttca gcaccaggct cactccatat
    11041 ccgtacctcg cctccctgcg gcgtgttctg aactgcattc aatatgacat tggatagtgc
    11101 cttttggagc agcctcggat cggacaggac aatttgcccg gctggaatat ctgtgacgaa
    11161 ccgctggttg tttgcctctg ccaaggtttg aaaatcgggc agcaactcgg caaccgtgcg
    11221 ccctatgtcc aacggttcag ctatgggtac gattctccca tcattcaggc tgacaagctc
    11281 cagtatttcg gaaatgattt tgccctgcct atccatcatt ttgatgcatt cgcgcagata
    11341 cttagaatga tctttgtagt caccgatatt ttcaagcatt ccctccaaca gaacgcttgt
    11401 agccgcgatg ggcgttttta gctcatgaga agctgccgca aagaaatatc gctgtgtttc
    11461 ctccaactca tgttcccttg cgatttcatc ctccagcctt gcgatggttt ctttcagcct
    11521 gacatacatg gaatgcatat catgagccag tgcgccaagc tcatcctttc gctccagcgg
    11581 cggcggtaca tctttcaggt ttgccatttt attcgcactg tccgctaagg ctttgatcgg
    11641 cgttgtcatt tgccgcgcaa agatatacgc gcataaaagg ctgaatacaa ccattatcgc
    11701 aatcataaca attccccgaa ttgtcagccc ttgataaagc aatcccacac ctgccttgct
    11761 ttgagcaaca atcgaaatat tatcatctct atgtaccaca taaagaaagt cgggtctaaa
    11821 actatttgat gtattggcat ttggtgtggc atagagtacg cttccctctt tatcttcaat
    11881 ataaaactca aaggattggt tattgtagtg aaacagccct gccacctctt gaatatcaag
    11941 cctatcgcta ttctgaatca gttccaccaa tggctgatag gattttactg tttgctgtaa
    12001 ttccatcact ctgaaataag acacaaattg ctgtgcaaac agtgttgctg ttacaccgac
    12061 aagcagtagc aggacaatga tcgtatagga aaaaacctta atgaaaatcc cttttctttc
    12121 cattattatg attcctccaa tcggtatcct acaccgcgaa tggttttgat gatattttcc
    12181 ggcagcttgg cccgcaaatt tttgatatga gtgtgggagt gtggactgtg ctgccatcac
    12241 cctcaaagtc atatccccat atacgtgaca aaatgatttc atgcgttaag gttctgcctt
    12301 tgttctgcac cagcagcaaa aggatttcaa attcttttcg tgtcaggggc agctctgtac
    12361 cgtcacaaag taccgtgaaa tcctccggca gaagtgtcag cctgccgaca cggatttcct
    12421 ttgccagcgc accgctgcgc cgcagcaggg cttccacccg ctttagtaaa atctgcatct
    12481 tgaatggctt tgttacatag tcgtctgcct ctgcatcaaa cgcccggatt tggttttcgt
    12541 catccgacag ggctgtcatc atcagaatgg gagtatcatt tttcgcacga aattcacgca
    12601 aaagttcatg cccgttcata ccgggcagca taatatcaag aataaccagt tgataagtgt
    12661 tttcgtaaaa cttggtgtat gcctcatttc catctgtgca ggcatccacc tgatatcctg
    12721 cctcagccag aaacgccctt actgtattac agatatgatc atcatcctcg acaagtagaa
    12781 ttcgtatcga cataaaaatc cctccatttt aagaagataa cataacagtc tgtagtttcc
    12841 ctgtagtttc aaagcgattt cttttcggat tttaatcatc cgtcattggg atactcgctt
    12901 cttttcatgg tgtatgtatc tatattattg gtttaaacga attaaatcaa gataataata
    12961 attaacacct tcttaatgtt tgggatgttt cgattacagc ggttgtcaaa acatcccgat
    13021 aatactggca agcaatggat cagaatatcc tttacggata ttctgatcca ccacttgccc
    13081 gcctgcggcg ttctcgtttt gcttgttggg gacaccccaa gcctcagcaa aatgcgcggc
    13141 ctcttttttg cgaatgcacc ttgcggtatg gcttttcaca caggcagggc ctgtgtgtac
    13201 tccctccggt cgctcttgcc gcactccgcg cagcagattc agtcctaata ccgttcaaga
    13261 cagtagaaaa tcagcaagtc tatattcctc atatcttcat catccgtgtc cttgcagtta
    13321 caggaaatgt aataatcgcc aaatgtaaat cccagaatat tttcctcaaa aagccgtacc
    13381 tccccggtat taaaatcaat caccccatac tcaaaggcgc attagaccag cagttatcca
    13441 atgtctggag acttcaccat aaagaatcca gatgccggcc ttttcaattc taccatatac
    13501 ggctgataca gcagcagctt cctcgtcttt gtttcgggca ttttccattt ctgtcatgat
    13561 aaagtttcgc ttcttcatac atccatgtgt aaatcttttc tttctgtttc agatgagaat
    13621 actttttatc ggtctggagc agcctgccat ctatcatctt atagtttttc cgtgacaaaa
    13681 taccatgctt tctctctctt caaaaccata tccaaaatgc gccggtttca ttatactatt
    13741 cagtaaccgc ctttcctttc tttgtacgcc tgtcaatcgg cttttccgca gttttgggaa
    13801 tgagccagat cgtcgccatc tttacagcgc cggggatgcg tcccgcagca caatagtagt
    13861 ttatccatcg tgggggcaca ccccatttct cgcctgcctc tttcaacgtc atgtagtcca
    13921 tagtacacct ccacagggta cattataatt cttttgctcg aagaataaaa gaaacaaaat
    13981 gtgaaatata aactttaaga aaggtgcata taccaacatt ccacgggcag agtatgaaat
    14041 atacaatgta agtgggaggt gcgatatggc gaatattgaa gattgtcccg gctttgaaac
    14101 tttcggcgcg gatgtgaaag cggcccggca agcaaagcgc gtttcgcgca aagcaatggc
    14161 agagaaaata aatattgact ggcgctatct tgccaacatc gaaaacgacg gggccattcc
    14221 cagtctgccg gttgtcatac agcttattaa agcctgcggg cttcctgtgg aacgctactt
    14281 taacccggag atcatgcggg aggaaagcga actgcggcaa agggtcagcc acaagctgaa
    14341 actctgcccc gaagaatacc tgccgatcat cgagggcgcg attgacagcg ccatactaat
    14401 tgaaaagccg aagaagaatg aaacggagga tgtgtaactg gcatcctctg ttttactttt
    14461 agcgggagct gcacagcgta gcttctattt ttttgcactt ttttctttcc agagggctat
    14521 tttgcgggta gtttggggct ttggaaacgt cttgttagtg gagaaacagt aacgtagcaa
    14581 gcatagggag tgtagccact cccggaaaaa cggcctgccc cgtccctgcc gggccgcgtc
    14641 cgttttgctt gttggggaaa tccccaaacc cctgaatgga aaggacggtg aagcatggca
    14701 aacagaaagc gaaaaatcgt gctgcgcgtc cctgtgacgc cggaggaacg ggctatgatt
    14761 gaacagaaaa tggcgctgct gcatacgaag aacttttcag cctacgcccg gaagatgctc
    14821 attgacggct atatcgtcaa catggacacc agcgacatcc gggcgcagac cgccgagata
    14881 cagaagatcg gcgtcaacgt caatcagatt gcaaagaggc tgaacggtat ggggccggtg
    14941 tacgcacagg acatcgagga tataaagggg gcgctggcgc agatatggca gttacaaaga
    15001 tacatcctat caagtcaacg ctgaaaaaag cccttgacta catcgagaac ccggacaaaa
    15061 ctgacggcaa gctgctggtg gcctcctttg cctgctctta tgaaacagcg gacattgaat
    15121 ttgaaatgct gctggcgcag gcataccaaa agggcaataa tctgggccac cacctgatac
    15181 aatcctttgc ccccggcgaa gccacgccgg aacaggccca tgagatcggg aagcagcttg
    15241 ccgaggaagt gctgcaaggg aaatatccct atgtgctgac tacccatatt gataagggcc
    15301 acgtccacaa tcacattatt ttctgtgccg tggatatggt aaatcagcgc aagtacattt
    15361 ccaacaaaca gagctatgcc tatatccggc gcaccagcga caggctgtgc aaggaaaacg
    15421 gattgtccgt ggtgaagccc ggccagagta agggcaagag ctatgccgag tgggacgccc
    15481 agcggaaagg gacgagctgg aaagcgaagc tcaaagcagc cattgacgcc gctatcccgc
    15541 aggcaaaaga ttttgatgat ttcctgcggc tcatgcaggc gcagggctat gagatcaagc
    15601 cgggaaagtt tatttctttc cgcgctcccg gacaggaacg ctttacccgc tgcaagacat
    15661 tgggggaagc ctacaccgag gaagccatca aagagcgtat caaaggccgc gttatcacca
    15721 gagcgcccaa agagcgcaag gggatttctt tgcggattga tctggaaaac aacattaagg
    15781 cccagcagtc ggcaggctat gagaggtggg cgaagctcca caatttgaag caggccgcaa
    15841 agactttgaa tttcctgact gaacatggga tagacaccta cccggatttg gaaagcaaag
    15901 tggccgagat caccgccgcc agcgacgagg ccgccgctac cctcaaagct atggaacacc
    15961 ggcttgcgga tatggcggtg ctgataaaga atatttctac ctacaagcag ctctggcccg
    16021 tggctatgga ataccgcaat gcagcggata aggcaaaatt ccggcgtgag catgaaagca
    16081 cgttgatttt gtatgaagct gcggcaaagg ccctaaaggg acagggtgtg aaaaagctcc
    16141 ccgatctgta cgccctgaaa gccgagtaca agcgattggc cgaggaaaag gaacggctgt
    16201 atgagaagta cggcgaagcg aaaaagcaga tgcaggaata cggcatcatc aagcagaatg
    16261 tggacggcat tttgcgtatg acgccgggaa aggaatggac gcaagagcta tgagtattcc
    16321 acgaatgaat taccctcaat atcgcaaggc ccggaagctg acgcatgagt gctgcaacta
    16381 ttgtgacggc aactgcctgt tgctggacga cggcgaagaa tgtatttgcg tacagagcat
    16441 ttcctattcg cttctgtgcc gctggtttaa ggccgctgtc ctcccgctgg atgcggccct
    16501 gtatgccgag atcaccaaag accgggacga agtgaaacgc tgctgtgagt gcggggccac
    16561 gttcacgccg aagtctaacc gggccaaata ctgcgcggcc tgctccaaac ggatgcgccg
    16621 ccgcaaagag gccgagcgcc agcggaaaag atactggaaa cctacgcact tagagctgta
    16681 aaagccctta tatttcaagg cttttccggc atggtcagcg gggtatgtgg gtgttttatc
    16741 ctatccccgc cgaagtgctg ttctaaatgc gtagaattta ccagcagaaa agcgccgttt
    16801 tctctggttc catgcttccg aatatggtat catagaaaaa atcagtctga cgggggtatc
    16861 aggatggacg gacaagaaaa gctgctggac tatgaaacga tcaaagccgc cgttgccggt
    16921 gagaggtggg ccacggaaaa agtgttggcc cattatgctg actacatcaa cgagctttcc
    16981 acggtggaga tcagacaggc aaacggcaaa aagaagaaag tcattgacga agatatacgg
    17041 cagcgcattt ccctgaaact gctggaagca ttgccgagct tcccgctgga acagagatag
    17101 acggcaagag ggctgcaccc tcttacgccg ctttgcggct atcacctgca cacccctgaa
    17161 acagatacac ccgctgcgcg gctatatctg tttcaggggt ctaattatag ctgcaaccta
    17221 cgcatttaag ggattgaaaa ccgcccgctg caaggcttct cccgcatgtt tgcaggggca
    17281 agcgaatact tcatcctatc cccgacaata ccgggttcta aatgcgtagg aataggcaaa
    17341 aagaaaagac gccggacgca agcgaccaca tggccgccgc gcccggcgtc tgctgttcgt
    17401 tattcgtctg tgaagtggaa cttctggccg tcatagtcgg catccgtcat gcgttccagc
    17461 ttggaaaggg tctgctgggc ctgcgcccgc atatccccat ccataaccgg catgacggcg
    17521 gtgagcctgt ttatcgttct gcgccggtct gcattgtggt acatacagat caggttttct
    17581 tcctccacgg taaagtgcat cattccttat acctccaatt cattcttttt ggttctggcc
    17641 gccgcctttt ttgggctggc ggcctgtttg ctgtcggata gctgcttgcg gatagagggc
    17701 cggggcctgc gaatactcac atcccggcgc tcgtcccggt taatcacggc atacacgcca
    17761 tgcctgcctt tcatcccggt aaaacacagg gatttatagg gcagcatggc aaagagccgg
    17821 tcattatcct tggtggaagc aatcgccgca aagtgcgggg agatctccac catgaaatgc
    17881 gttttattgg ggctgttagg cgcggacaga tctttcaggc tgtccacaat acgccgggcc
    17941 tcggtttcaa tccggctgtc atgcaaagcg gtgacgtgga ctttgaaatc gccggggacg
    18001 gcccgccccc ggctgtgctg ctccgtccgt agcgcctcct gcagcacagc cgggtcatgg
    18061 ggctgggcct catagtacaa aaagtttccc aatacccggt cagggcttcc gtcaaaacgc
    18121 tggctctccg ggatgtcacg ggtgccatgc tcataaaaga gccggttcac actcaccggc
    18181 tgggcggttt caatgacgtg ccggaaatgc tgcggatagt ccagctcata gagattgccc
    18241 cggatagtgc cgccctgtgt gccggtcagc tccaccgcgt aggccagtat cttgtcacgg
    18301 gtctgctccc cgtaaaattt ccatgtgttg tactgtcggg tgtctttcag gaaaacatca
    18361 cgttcccgga aacagtacgt cccggagggc cgggacatcc agagcagggt cttgtctgcg
    18421 gcctgatcgc tggcggcatc ccgcctgata atgtctttgt caatgccaaa gtcactttga
    18481 taaaacgcgg tgttctgccg catgatctgt tccagcgcgg ccagaacgtc cacattttca
    18541 aacttctgca tttactctac ctccaatccc tttgattttt cccgcgccgg tggctgcttg
    18601 gcctgttcgg tcttggccgc gttgagctgc gcccggatag agggcttttc tttctccctg
    18661 cgggccgcaa gctgtaaggc gctgggcttt tcgccggaaa ggggctggat gcacttgata
    18721 cggtcagcga cgcggattgt ggtgtcgttt aactgccgct gccatgcccc ggccttgggc
    18781 gaccagcgga aaccgttgct tttcagctcg tcgcgtacag cagcatccgg cttcccctca
    18841 aagaagattt gcaggcggtt ggccacccgg ttgatctcca cagtgccgcc gtcaaactcc
    18901 catcccacat aggccgcctc cttttggcgg gtcaattcct caatgcggcc ctttaaccgg
    18961 cgtatctcgg cgttattgtt ggggagctgc caggactgga acggtttttt ctcatagtgc
    19021 cagcccgacg ccatatccgc tttcaggttt tcaatgcctc tttcggtcag atgggggcat
    19081 ccgtccagcg tcccatgctt gcggtagtag gcattgacgg ccttcatcaa ctcctgcgac
    19141 gcttccagct tttccagttt cttttccagc ttggaaacgg cctgcggatc gtcggcgctg
    19201 ataccaccca tgccggtgct gcggattttg tcaagcaagc cctgaatgta ctgaaattcc
    19261 tccatgttgc ggtcactggc ggcgttctgc ttttctttcg cccgtaccgg gaaattggac
    19321 ggcccggcaa tcaggacaga ggggacgcgg gcggtaatct catagccctt attcatgttc
    19381 tcggccagct tgcgggcgta ggtgtcaagg aggctgtcta tcttttcgtg atactctggc
    19441 tccacacgct ttttctggcg ctccgcaatt tccacggcct gatcgacata gtgccggtat
    19501 tccgccgtgg cgctgccggg ccggtagtca gaaaagctca tggcttcctt ggcgcggcgg
    19561 gcggctccct cattgatggg ataatagcgg acagccgttt gcgggggcgc agcctccggg
    19621 ggttcctcct gtgtctggcc ggaagcaact gccacatcct caaaagatag ctgttcgggt
    19681 tcctccggct gcggttcggg cggcgtattg ttgataatgc cgtcaatcat gttttcgttc
    19741 tgctccgtag acagttcggc ggtacgcagg ggattttccc gcaaaaactc cggcacttcc
    19801 atatagccga agctgtctac atagtgggcg gtgtcctgtc cattctggtg cagcaccacc
    19861 acgtcggaaa cggaaaggct gtgtccccta aaatctgccg ggtggtcgat gttgaatgtg
    19921 cggtaaatat cctccaaaga cgtgctttta tccagcggtg ccgtataaac aaggtcatag
    19981 ttggcactgt ccacggccag ccctgcggcc tgtaaacgct cgtatggctc aaaccgatag
    20041 tcgcgggttt cctccccgcc tcgaagctgg tagatggaaa aggtgtcctg ttggggtgct
    20101 tcatagccag ccgccgcgta ttcctccacg gtcataccgg ctgcggcggc ctcctgcgcg
    20161 atctcggcct gtacctgtgc tttgtagtcc tgcaagctct ggtcgaagtc agccagtttc
    20221 ggcggctccg gcaactgata ctggccctga ttgagcatgg ggcggcactc ctgcacataa
    20281 gagagaagcg cccccaaata ggccgtttcc tccggcgtca gggtttcccc ggactgtaag
    20341 cggccctgcg ccgtctgttc catttgggca aggctgcaat gcagcttcat ataggggaca
    20401 aactcgccta acagcatatc ccgatgggcc ttgtcctgct cccagccctc tatgccggtt
    20461 gtagcgatta catgcttttt ccagttttcg tcctgcgcgt aaaattggtg gtagctctgg
    20521 atatgttcga tcagggaacc gtccctatct ccaaaatcct gccgcccctc gtagctgtcc
    20581 ggctcgccct gaaagataca gtcaatgcgg aacgcggttt tgtcatagcc tgcctctgcc
    20641 ggatatgctg catccagacg ggcaaacagc gcgtcggcct catgcaacgg catttgctgg
    20701 ccgtccttta gctgggggct ttcgctccat agcacagtca caagcggctc ggctgccgga
    20761 tcaagggccg gggcctgttc ttcctcggcc tgcgccgggg ccgctgcctc cggggtaacg
    20821 tcaaccgtga cggcctcctg ttcctgccgg gcctcgcgct cctgctgaag ctgggcaaaa
    20881 tggccgtcga tctcggttat caattcgctg gccgtggccc ggatggtttc aagggaggct
    20941 ttcagctcgg ataactccct gccgctgctc caatcggcca catagccaaa agaatagtcc
    21001 gacgtgtcca gcccgtaata ctggcagacg gtataggcca cgctttcggc ctgtacctcg
    21061 cgggtgctgc ggtctggccg ggccgcctgt tcctcggcgg gggcctcccg gtcaatggcg
    21121 tggagcttgg catgggcaat ctcatggatg gcggtcttta gggtctgcaa ttcgctcatg
    21181 ccctcgttga tggcaatgcg ctgttttgcg tagaagcagc ggcccttggc cccgtctgaa
    21241 atcggctcaa tgcttatggg atagggtgat gtccgttcca gcgcggcaaa gaaatcctga
    21301 tactgctgca cgtcgccggt aagttcactg atatagggca tgggaaaatc cttgccctcg
    21361 gtctggctga tgtcaaagac tgtcaccacc ttaaaggccg gaaccgtcac ttcctgttct
    21421 tcggtcacgg gcttgccgtc cgcgccgatc acgggctgct gggtggccgg gtcgattttc
    21481 tccatctgct gcttaacctt gaacggggcc ggggccagta tcttaatgcc tttctcgccc
    21541 cgcttcacat ggcggtcaaa ttcatcctgc cacctgttat agcccgccac aatcgaaccg
    21601 ccctgcatgg cgatcagcag cgtattgttc agactgtaat cgtgcatctt ggacatagcg
    21661 gataagtaag ccatatagcg gctactttca aaaagctcct gtacgccggt ttccagacgg
    21721 tcagtaatca ctttcatgcg ttctttgctg ttcatgccgt ccagcatgat cgggataacc
    21781 gggggcggcg ctttgtcggg gctttcggat gcctccggct gctgggcctg cggttcctcc
    21841 cggtcagctt ccggctgggg gtagtgcatc acccggtatt cctccggcac cggctggccc
    21901 tcatggacgc gctgccattc gtcgccgctt ctcaccagat agccgtactg ggtaaaaacg
    21961 ccttgttcct gctgggcaat gtggcgtcca aactgcgccg tatcaatccc ggctttccat
    22021 tcctccggca tatccaccat gccggaacgg ttcaggtagt agtcgcccaa ctgtccatcc
    22081 cccctaacgt cgggaagatg cacatagtaa tccacatttt cggtgtagtc gatgatctgg
    22141 ccgatattct caaagccgcc acggggattt tgcagggcgg cgttcagctc tgcgatctcg
    22201 gcggcgtcaa gctggcccag ccgggcggca aggaaattca gctcgtccac gtcggcggcc
    22261 agcaccatgt catagggaat ggcaaggtgt ctgtcctccg ggcaggaata cccgttgata
    22321 aaaaaatcct gcggattgtc ggcagtaatg ccaacatccc gcagggcggc ttgcagcgtt
    22381 tcggttgtgg tgggcaaatc cagccagacg ccttgtttgc cctgttccaa aaactgccgg
    22441 ttatggactt ggatagaaaa tacttcgttc acgcggtcac tcctttctct gttctgtttc
    22501 aaaaaatagg gggttgggga cttccccaac aagcaaaaat ccccggcccg gcagggacgc
    22561 ggccggggga ttttaccggg agtgtagcca cactccctat gcttgctgtg ttactgtcct
    22621 ttagctcctg tgttcaatgc ggtagttgac gtacttgccc ccggtatcag ccaaaagcac
    22681 cgtcccgtcg taggttttgc cggtctttac ggaatagagg ccctttacct tgactttgcc
    22741 ggttttgagc agcgcagcgg cgatcttcgg ggtgaacgct ttcttgcgtt cctcaaaaaa
    22801 tcggtcattc ttccacatga cgaactggca ggcccggttt ccgcagtagt agtttttctt
    22861 gccctcatag acagcctcac cacagcgggg acacgtcccg ataggaacac gctcggcctg
    22921 aaacaatttc tgcttttcct cactgataca ggaataggtc tgcaccagct cccgcgcctg
    22981 cgtctcgatg ccgtccataa attcgtcagg atcggcctgc cccttggcga tctcggtcag
    23041 ccggttttcc cattcggcag taagctgggg agaagtaagc gcctccggca gcaccacagc
    23101 cagattgatg ccgtccttgg tgggaatgag ctgcttgcct tttctctgga caaagcccat
    23161 ctgcaccagc ttttccagaa tggcggcgcg ggtggccggt gtgcctaatc ccttgcgctc
    23221 ggcatcctcc ggcatatcct cggccccggc ccgttccata gcggacaaaa gcgtatcttc
    23281 ggtgtagggc ttgggcggcg tggtgaaatg ctcggtgaca gaggcggcca cgtctgcaaa
    23341 ggtctggccc tcgcaaagct ccggcagctc ccgcaccgcc tcggcgtcct cgtcggcgtc
    23401 ggtctttaag gaagaacgga accggcgttc aatctctttc cagccggggg aaaggacggt
    23461 cttgcccttg gcggtaaact cctgcccggc acaggtaaat gtggcggtga cggcctcata
    23521 gatatgcggc ccggccacgg cacataagag cttgcagcag atcagggaca gcagcttttt
    23581 ttcactctcg gccagagtgc caaagccctg cttcacaaat tcagcggtgg ggataatggc
    23641 gtggtggtcg gacacctttt tgctgttcag gatgcggtca aactgcgggg aaaggttcag
    23701 gcccttggca aacggcacca gctcccacag gccggacaca aggctttcca cggcgggcag
    23761 catatcgtcg gtaagatatt gactatccgt tctcggatag gtcagcagct tctttagata
    23821 cagtgcctgg gcatagtcaa gggtctgctt ggcggtatag ccgaaaagcc ggttagcctc
    23881 ccgctgcaag gtggtaaggt catagagctt gggcggctgt tccgtctttt tctcccgcgt
    23941 cacggaaacg caaacggcct gcgcttttgc acaagccgcc ttgacggtgt ttgcctcggc
    24001 agcgtcggaa aagcggctgc ttaccgcgtc cgcgccgccc gccgtgatat ggacgatgtg
    24061 atatttctct ttcttgaaat cggtaatctt ggcgtccctg tccaccagca tttttaaggt
    24121 cggcgtctgg acgcggccca cagtcaggtt cttgtggtac aggacggaaa aaagccgggt
    24181 ggcgttaatg ccgatcagcc agtcggcctt ggcccggcac agggccgatt gatacagcgg
    24241 atcatagtcg ccgcccggtt tcaggtgggc aaagccgtcc ttaatagcac tgtcctccat
    24301 agaggaaatc caaaggcgct caaagggctt ttggcatccc gccatctggt agacgaaacg
    24361 gaaaatcaat tccccctcgc gcccggcgtc ggtggcgcac acaaggccgg tcacgtcggg
    24421 gcgctgcatc agcgaacaga gaacgtcaaa ctgctgtttc ttgtcgtcgg ggacgatgta
    24481 ctgccatgtt tccgggagaa tgggcaaatc ctcatagcgc cattttttga aacgctcgtc
    24541 atagcgtccg gcgtcggcaa aggacaccag atgtccgatg caccagctca ccagatagcc
    24601 gttcccctcc aaatagccgt cgtgtttctc cgtggccccg atcacgcccg caatgctgcg
    24661 ggccacgctg ggtttttccg cgattacaag tttatatgtc attcctcgtc ctcctgttca
    24721 ctcacaaaat cagcttccgg gtcaaactcg gtttcgggtt cctcgtcctc caaatcctcg
    24781 tcctccccaa aatcatactc gcccaaatcg tcgctgccgg tggtttcagc cttgggcttt
    24841 ttgaacttga aatagtaaag ggccgcaccg ccgcccgcca gcgccaccag caggaacacg
    24901 atcaggccgc ccgcgccgcc cttgggttct tcctccgggg cttcgggttc ctccggctgg
    24961 ggttcctgcg gctccgggcc gctgcactcg gtcaggttgg ttttacatac cgggcaattc
    25021 gtgttcacag ccccggcctt gcatttcgtg gtacaggagc agacagcagc gggggtgacg
    25081 gtttccccgt cctccatcag cgtcagcaga tcggcttcat ccacctgatt taagaaatgg
    25141 acagtttctt ttccctcgtc gtcccggtca ataatcagat aaaacacatt gccgtttttg
    25201 gtttccacgg tgataaactg cttgccattt ttcgcggcgc tgccgatgtc gtcaatcagt
    25261 gacaggttcc cgtccgggga cagggcggcg ctggccccgc cgccattgaa cagttccagc
    25321 agagaagcaa tttcctccgg gtcaatggag agggaaaaac cgccctcgcc ggtagccgct
    25381 acttcctttt cgctgacggt catatagtcg gaatggacat agccgatgcg ctccggcaag
    25441 agaattttaa tccagtcgcc gtctgtgccg actacctcaa ccgtcgtccc attgggaagc
    25501 tgggtgaacg cggtcttgtc aagccccgcg ccggtgcgga cattcagcct gccgccgttg
    25561 gtggtgacgg tgccggtctg ttcaggttct tttttgtcgg tgccggtggt gatctggatg
    25621 ccgtcgtccg tggcggcaat gctcaccttg gaaccgaaca gcgcggaaaa caggtcggtg
    25681 atgtccttgc cggtcagagg gccggtatct ttttcggcct cggtttctgt cccgtccgtg
    25741 gcggtgccgg tttcgtctgt ttcaggctcc aaaccgcccg tagcgggttc ggttgtagcc
    25801 tccggcagcg gggccggtgc cgggtcattg ccgcctgcaa gggcggtaac gggaaaggcc
    25861 gtgacgcaga gcatcacggc cagaagtgcg gggagaatac gggaaatccg tctggtctta
    25921 ttcttcatgg gcattgtcct cctgttcatg gtaaggggtt gcggcagggg cggcctgcat
    25981 tgcgccgctg cgtaagaacg cggcaaattc ctccggcttc atgttcatgc cgcgcacaag
    26041 ctggatgatc tgcaaattct cctgttcggt tttctgcgtt tccagctccc gaagctgttt
    26101 ctgaaactcc gcgatcttag acttggtttt ctggatgtcc ttttcgatgc ggtcaagttt
    26161 ggttgccata attgaaagct cctttctggc gttaaggtaa acgcccgtaa ctgtaaaaat
    26221 gggattgcca gtagtttgaa gtaaggttcg tgtaggaaat cgggttcccg caatgcagca
    26281 tcacattgtt gcccacatac aggccgacgt gggacacgcc gggggtgtcg taggttccct
    26341 tgaaaaatac aaggtcgccg ggcttggcct gcgaactgga aacaggggtg cagatgttat
    26401 ataggccctg cgcccccagc cgtcccacgt tccatccact atgattgatt acccacgaaa
    26461 tgaagccgct gcaatcaaag ctggtgctgg gagagctgcc gccccacaca tagggataac
    26521 cgatatattt ttccgcctct ttgagcatgg cggcaaaggt ggcatcctcc aaagcctccg
    26581 ggggtacgtc gtagtaggtc ggcggcgtca gggtggaggc gttggggtac tggctgccgg
    26641 aaaacagatc aggccggttt cctagcgtgg acatatacag ggaatagaaa cttagcgttt
    26701 cctcgtccat gatatagact ggcacatggg acaggtcgaa gttttccagc gtgacgtagc
    26761 agatgtaata gtcatacggc acttggtagg tgtctgtatg ggtgttgccc tccgcgtccg
    26821 tccatgtatc ggtttcggtg cggtaacggg tttctaccac gacgttttct gtcaggatat
    26881 attgcttgtc aaagagcatg gacaaatcac cctgtacctc cccgatggta aatatcccgt
    26941 catggagcgc cgacagaatg gaaatcagca cataggggtc atggctgatc tcgtcaaggt
    27001 caaaatggta ttcgtcatag tcatgggtgg cctcgtagtt gtccagatag tcttgcagct
    27061 cggcttccag ctccgcgtag gcgtcctcgg cggccaacat atcgctatcc tcggaaaagt
    27121 aagaggacgc cgtgatgccg cttccggcgc tgccgaacat ggcggtgcag gactgtaagc
    27181 cgccgatgat aagcagcatc aaaagcccca acacaaggac ggccagcgcc cctttccagt
    27241 ggcgggccac aaaggaggcc gcccgttcac tttctttggc tgcggccttg gccgctttcg
    27301 tggaggcccc ggctgtcttg gccgcgccct gtgccgccgc gcctgcggtc tggcccgctg
    27361 tccgggcggc cttggcgtac tggcgcttga tctgctgttt ctgccagaac cgggaaatgg
    27421 ggttgctggc cgcctgtgcg atctccgggt tatcccgcaa agatttctgg tagaaatact
    27481 ccgcgttggc agacaatagc ctgcgctcgg ccttttccgc gtcccagtag ggtttcagcc
    27541 tgtggtgccg gatggtgctg cggatggctt ttccggcctg ccgctcggca agttcctccc
    27601 ccttatggcc gccctccaca cccacgtttt catgctcgac ttcatggatt ttcccgtggc
    27661 cgaatagaaa aacctcggaa acaggacggc ctgccggatt gggtttcagg gagggaggcc
    27721 ccttgtcctg tttgtcaaag tgcaggacgg tctttgcttt gccgcttgcc tcgtcaaaga
    27781 cggtttcctt gaccgggacg cgcttcttgg ggatggcggc gcgggcctta tccagcttat
    27841 cggcccgttt gtccgatttt cggatatagg gctgtaaatc cgggtcggcc cgttcttcct
    27901 ccgacagttg gagccgggcg gatggccggt gcagggcagc gtccccctcg gcggccacag
    27961 ccccggcctg cttcgcggct ttcttggcct tatggccgtc atggatggcg tcggcccgtt
    28021 ccagcagctt atcggcggtg ccgcccggcc ccggcgcgga caaatcctgt tcggcctccc
    28081 ggctggaaat gttctccacc tcgccggtgg cctgattttc cagcgtcagc ccgtcgcggg
    28141 tcatggcctg cgtcactttc gccttggctt tcagctcttt catggtggtt cacctccttt
    28201 gtggcgtcgc cgggtcatac tcccgccacc tcgtttaagc gggtcgtcat aatcttgtaa
    28261 agctgggtgt tgtgcgggat ggggttggta aagggcagca ccacgttttc aaagatcaaa
    28321 aggccctcgc cgggttcgga attgtcgatg tacttctgct gttcggtgga caaattcaag
    28381 cgttccgcaa ggatttttct atccccggac gcctgattga gcagcgtaat gaagtcgctg
    28441 ttttcaagga tattttcaat ttccggggag gaaagcaggt ctttgacgtt ctgggtggca
    28501 cctgttggaa tgccgcccca tttcctaaac cgcttccaaa tctccgcaga ataggccgcc
    28561 gtctgttcct ctttcaaaag caaatggaac tcgtcggcaa aataccatgt ggcctttcct
    28621 tggcttcggt tggccgtgac gcgcccccat atctggtctt ggacaatgag catccccagc
    28681 tttttgagct gcttgcccag ctccttaatg tcaaaggaca caagccggtt gccgatgtcc
    28741 acgttggtac ggtggttaaa tacgttcagg gagccggaca catacaggtc aagggcctgc
    28801 gccacccggt cagcctccgg gacatgctga tctaacaacg ctttgtggag gtcggacagg
    28861 atcggcatat tctccgggcg ggggtctgcc agatagggcc ggtagatcac gcgcacagcc
    28921 cggtctatca cggtcttttc aatggcttcc agcccgttct tgccgcccat gacaagctca
    28981 cagaacgaca gcacaaagtc ggatttcagg gccagcgggt tatcgtcctc cgaataattc
    29041 aggttgatgt ccagcgggtt cacaaagtcc ttgctggtag gcgacagccg gattacctgt
    29101 ccgtggagcc gtttgacaag ggggtaatat tcggcctccg ggtctgaaat aaaaatatcg
    29161 tcaggcgtcg ttaagaacac actgacgatc tcggatttac agctcatgga tttgccgctg
    29221 ccgggggtgc cgagcttcaa ggcattgggg caacgggcct gcttcctgtc cagcataatc
    29281 atgttgcggg acttggcgtt gatgccgtaa tacatggccg cgccgccctg aaaaagctcc
    29341 tgcgtcacaa aggggacgaa cacggccacg ctggaagtcg taaggctgcg ctgtatcttg
    29401 atctgattga cgcccagcgg caggctgctt gcaaggccct gttcctgctg gtagtccagc
    29461 cggacaaggg gacagttatg cttttgggcc acgcctgcgg cttggaacac gtcgttattc
    29521 agcttttgct tggtgtcggc cacgttgagg acaaggaacg tcaactggaa cagccgttca
    29581 ttgcgggtct gcaactttgt cagcagcttc ttggcgtcct cgccgtaggt ggcaaggtcg
    29641 ctgggaagaa tgtccatatc gtaaccgctg cggacggcct ttttctgctc cgcaatcttc
    29701 atagcgtcaa ggtcggtaat tttgcgcttg atggtcttga tcgccttggt ctgttcaatg
    29761 gcctgcacat ggagattgac taaaacaccg ctttccgtat tgaggaagtc ggccagcata
    29821 tcgtccgaca gctccgggga caggatttgc aggaaactta ccgcgccgta tttgccgccc
    29881 atgccgaatg tctttgcatt gccgaagcac agggaggacg gggcaataaa atccttggtg
    29941 gagaggccgg acggggccag ccacttccag tcaaaattga atatctcgcc gtccgggtgg
    30001 tacacgccat gcagcaggtt cagccgttcc cagccgtcca tcacatgggc tacggccccc
    30061 atgctcttga aatagcctaa caggtcaaag ccgatgcgct tcaatctggc tcgcgccgct
    30121 ttcacgcttt cggcctcgat ggtgaatgtc aggtatttgg tctttaccat gccgttgttg
    30181 ccgtttgcaa gctgcttttg cagaatgccg gtgtactcgg cccggatgtc gtcgaagtcg
    30241 tccccctgcg gcgcgatctc aaagctcttg gcgtactgca ccgggtcaac tttccgattg
    30301 aggaaagaaa actggatatg aatggaggcg tccacatagt tatacaggtc gcacagtttt
    30361 tcaaagatag ccgtctgcgt gtccggctgg gcaagctgat agctgatgtc cgcaaactca
    30421 atgcacttgg aaaagacgcc gggcgatacc cggcacacgc cgtccgggta catgacctcg
    30481 taggggatgc tctgctgcgc cgtgtggggc ctgccgtcgc ccttatattt acggattacc
    30541 gcgtcaattt cttttcgctg ggcgcgggtc agctttgttt tttcggtttc tcttgggatt
    30601 tcctttttgg acaatggcct ttacctcctg ttccatctgg tattgccgca caagggcggt
    30661 gtaggtgttg tcggtctgat agggacgctc ctttggctgg atgaagcagc actggatgat
    30721 ctgcataatc accacttcaa ggggctggcc gtgacgctcg tacatggcaa gcaaaaagcc
    30781 cggcagcatg gcaaatacca tgagcagggc cgccgcgctg ttggtaagat agtcacgggt
    30841 gagaaaatat aaggggacgc cgatcaccag cgccccggaa aaacagatca gttgccgttt
    30901 ggtaagccca aacacaacct tgcttttcac atgggttaag tccttgggga cggttacata
    30961 agccatttgg cacctccttt cacgtcagga taaggtcaca ttccacctcg ttcccccaca
    31021 cgtcccagcc gggggttttc tgtctggcaa acagctccac gcggggcaaa tcgcccgcca
    31081 gctctacgat tttttcacgg gtcacatccg gctttttgct gtgttgttca atcgggctga
    31141 taataaactg atggacagag gcagaacgac gcttcggctt gccgtgtgtt gccagcaggc
    31201 agatttccgc gtttcccctt gtccagcggc ccagcccgta aaaccatgta aggcttttac
    31261 ggttctgttt cagccataca aaagccacgg tcttgaatga aaagccccac gctttgatta
    31321 accgcagagc ttccggcagc atggggaatg tggcccacaa aaacagcagg cagtctttgg
    31381 acgccagccg ttccaccggc agggcgcaca gctcgtcaat gctcatggtg gaatagtgat
    31441 tttccgctgc gccctgtacg tttttgcagt cataccgcca cggcgggtca gcgtaaatga
    31501 tgttgtacgt tgaaataaac aagttcctcc tgtgtatgct tggattttgg gatcaatgcg
    31561 ccccgaatac ggcctttgat atactgccgg tcttgaacag gcagaagcac agcagcaccg
    31621 tatagcccat acagccccag attgcgccgg atatgtcgga agcggaaccg atattctgta
    31681 tcagcaccgc gtagataccc accacaacca tgattaaaaa ggcttggaag cccagcgcca
    31741 gcagggattt cagataccct tggcccatgc cgcgccattc cgcgttgccc attgtggcaa
    31801 gggggatagg ccccagcgac gtgacaacat agatttccac catgcggcca taggtcacaa
    31861 ggaaaatgca gatggaaagg atattcatgg tgaggcccac aacaagggtt tggaaccaca
    31921 gccccaaaag agcgcccacg ctcatagcct ccaattggct ttcaaggtct ggtatcaggg
    31981 tgttaaaatc aatggatgtc tgcccgataa tcacaccggc gctttgattg acgacgcttt
    32041 gggtggcgtc gaatacgccc atgacgatgt tccatgtgtt cgttacgatc aggattgcaa
    32101 acgcggattt gaatatccag cggaagaaca tggagctgtc aaagtcgtgc atattgttct
    32161 tttccgttat catctggata agctcatagc acatgacaag ggcgagtatg gccccggcta
    32221 tggggacgat cacggtttcc gacaggctgt gcagcatatt gaaaataccg ctgttccatg
    32281 cctgcggggt tcgtcccacg tcggcggcga tttctccgac tttggcattt accgtatcga
    32341 acagcccgga caggttgccc gtgataccgt ccatgaggat gccttttatc cattcttcaa
    32401 tgatttcact aatcacgggt agcacctcct ttcccgcgcc ctcccggtct ggccgggagg
    32461 acgcggaggc gattagttaa gggtttggac agggacaaag gccaggatca gcccagcagc
    32521 ccggacagca ggggaaccag agtgacaccg atcagggcca cgccgccgcc agcgacaagc
    32581 tgcttgacac cctgcgattt tgcccccgga ttgtcctgcc cgtatccctc caacagattg
    32641 atgccgcccc atacgcacag agcgccgccc agtccgatca cgatagtctg caaagtagta
    32701 actgcggaat taaaaaatgc cataaattta cctctttctg ccggtttccg gctcataatg
    32761 attttttgat tttgttgttg gaatgaaaaa agccgctgtt tattcagcgg cggcgtcctc
    32821 gtccgacagg tcgatttcat acaggtcgaa tacttcatcc tgcgttacca cttgctgccg
    32881 tttccggcgc gtgatagtca aaaatctgtc cacgtcaaag gtattcttct tgtcggcgtc
    32941 ggcaaggtat ttgtaccggg gatgcttggt aatatcgaat ttctccgata aaaacgggcg
    33001 cacaccccgt agctgcaaga tacacttgcc gccgtccatc acggcaattt cgtcttggga
    33061 catcaactcc tttcctaatt tttgatagtt caatccgtgg gacgtttggc ttccccggtt
    33121 ctcgctggtg ttgaaactgt caatcgtttc tttccccagc gcatcggaaa tctccttggc
    33181 atttttgccg cgcccactaa ggaaaagcac actatcgcag ttatccgaaa tgatctcggc
    33241 ggcgtccttg taaatcgctt ttagctgcga ctggctctgc aaaataatag aggccgatat
    33301 ttcacggctt cgaatggtgg caatgagttt atcgaaattg ggtatttggc caatgttggc
    33361 aaattcatcc aaaatcaggc gtacatggac aggcagccgc ccgccgtaca catcatcagc
    33421 ccggtcacaa agcaggttga ttagctggct ttggagcata gccagaatga aattaaaggt
    33481 cgtgtccgta tcgctcataa tgagatacag caccgtcttt tcgtccccga tggtgtccag
    33541 ctccatttcg tcgtcctcca tcagctcgcg tacctcccgg atgtcaaagg gcgcaagtct
    33601 ggcaccgcat gaaatcagga tggaagaacg ggtcttgcct gcggacagca ggaatttctt
    33661 gtactggcgc accgcaaaat ggtcggggtc tttctgctcc aaacgctcaa acatgagatc
    33721 gacggggctt tgaaactccg ggtcgtcctc gcgggcttca ctggcgttta tcatttccag
    33781 cagcgttgag aagttcattt cctccgctgg ggcctcgtac cagatatagc cgatcagggc
    33841 cgaataaaac aaccgttccg acttcaccca aaaatcctcc gctgactttt ccccctcgcc
    33901 cttggtgttg gaaatcaggg tatttaccag ctttagcacg tccttttccg aatggatgta
    33961 gacaaagggg ttgtacttca tggacttgga aaagttaatg gtattcagga ttttaatgcg
    34021 gtagcccgcc tgctggaaca tcctgccggt ttccagaacc aaagtgcctt tcggatctgt
    34081 caccacaaac gaagtcggat agtccgtggt cggaaagcat tgcatcaggt tgggtttcac
    34141 gaaaaatctc gttttccctg aaccgctgcc accgatcacc agcacgtttt tattccgcgc
    34201 tgtcttgggg tctttgggcc tgccggtcat ggtaagccgt tccgtttggg tgagcaggat
    34261 attattttca aacttggggt cgatgtaagg cgctatgtct ttggccgtcc cccagcgggc
    34321 gctgccatac tccatgcctt tgcggtattt cttcacgttc ttgctcttgc agtacaccac
    34381 cagccgcacg atcacggcac cggcgatacc gatacacagg tcaaacaggt gcaggcttgg
    34441 cagcgggttg gcaaaagccg ccgcaaaacc atccgcaaaa tgcaggagct tccccgacag
    34501 gtcagcacca gcggccagcc ggtacgcctc cccgcatttc gcaaacaggt acacaaaaat
    34561 cagatacggg aggttcagga tcagcaattt ctttacgtca ggcttcacag ttccagcccc
    34621 ctttccttga ttttctcctt ggcccgctgc ttggtctggg ccgccgcctt ttgcttcaca
    34681 gcggacaaaa ccttgcggat agagggccgc tgctccttgg aaagcgtctt ggcggtaaac
    34741 tcccgaaacg ccagatcaag gttatctgca tccttggcct tgaaaatcac aacgtagtgg
    34801 ggcggctggg tggtcttatc ttttcgtaag gtaaaatcca cgccgtattt cttggcgcat
    34861 ggcttgaacg ctccaatgtt ggcctcggtg atctcaatgt tggagagatc agccccatgc
    34921 tttttaagct gctttaggct ctgctggccg tggtgcagcg tgtttttgcc cttggcccgg
    34981 tgttgcaggt actttttcag cgccgcttgc agcacttgag ccgtgagctt gcccgttttc
    35041 atggacaggg caatggtctt ttgagtaact tcttcctgca atcgcatccc tccttgtcag
    35101 aaagtcaaaa gcggcgtcta aggcggaacc accagacgcc gcaggtagat ataggtcaaa
    35161 tccgcacccg caggccggaa aggtcgtcgg cccggatgcc gacaagatac cagttttcgg
    35221 ccatttcaat gcgggtgggc ctccatttgc cgccgatcat cacgtcgaaa cactccccgc
    35281 aatgcaggcc gccgtaatag tcggccagat caaagcggat gtcgtaacgg tctgttcgct
    35341 cgtcaaaaat caatgcgccc tgcttcatag ggtcgtcctc cttttctcgt tacaggcttt
    35401 cgccctcgcc gcaggaatag tccggcaggc cggatttagg ctttccccaa tcgggctgtg
    35461 ccatgtcgtg cgcgacaagg gccgtgtagt agttggaaat ggtgctgggg gcgttgaaca
    35521 gcatagcccg caggtattgt ttgatattgc ggactttcgt tgtgttctcc cgcagacagt
    35581 caaggacaaa gcggatatgt tcgccggtca gcttcatgta cttggatttc accagctcgg
    35641 cagggtagtc gtccccggcg acacggattg tctttcgggc ggtgcagacg gtttccagca
    35701 tcaggtctac gatttcgtca agctgttcac ggtcaatgct cctgtcctga atgagatagg
    35761 catactcgat gttctccaaa atgatttccc gataaatccg tacagcctca tcctttcctg
    35821 tccttttccg ttccggcggc gtagccgctg cctcgccctc cataggggca ggggtcgggg
    35881 atgggatagg aatggaatgg gtactttgtc catctgtaat tattttttct ttttttgata
    35941 agtcagttct ttgtacttct ttatttaatt gcgttggatt ttccgacgcc ggattatccg
    36001 atgtcggatt ttccaacacc gggttatccg atgttggatt ttccaatacc gggttttcca
    36061 ataccggctg ttcgtagatg gtatagacca tatcggccat tttgcctttt gcgtcacggc
    36121 cctgctcacg ccggatatat ccagcttttt caagctccca taccgctgtc ctgatcgcgt
    36181 cgatgctttc ccggttgata taggacagtc cttttagggt atagtcccaa tcctccggca
    36241 aggacaacat ctgcgacagc aggcccttgg cctttaaggt caggttctta ttccgcaggt
    36301 ggtgatttga catcaccgta tagcctttgt tcttttccac acggaaaact gccatagcgt
    36361 tgttcgctcc tttcactgtg aatttgaccg ctgcatagtc ggtcagcttg cccggctgat
    36421 agggacactc tgcaaactgg cagaattgat atttccaatc cgggcggtaa aatcggcagg
    36481 tgccgcaatc ctccggcgcg tccgccccgc cgccgtcgta gtgatcgaaa ccgggctttt
    36541 cctgcatcag ccgttcaaac gcccggtcag ggccggaagt aaatagcatg gcgtcgcctc
    36601 ctttctctat ttgggcataa aaaagcggcg tcctgttctc ccatgacggg aaagaggaac
    36661 gccgcgttta cggtgtcgta ttcaatttat gatgtgccgt atttggggtg gcgggtatta
    36721 gcagtatggt tcagcatata tgcggctcaa tccgaaagta gtaaattggt agtaaattga
    36781 gttctcatat cctgcgaaat gcccgtattt ccggggtttt cagcgataac ctaaaatttt
    36841 tgaataaaaa ataatgtatt aaaatgtatt aactcgtttg tcccaacttc caaaacatat
    36901 ttttgaaagt tgggtgttat cttttttgaa gtataaaaag atctcaaatc aatccacttt
    36961 actttcttat tatgttattc a
//
LOCUS       DAZUKD010000016        36188 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000016 DAZUKD010000000
VERSION     DAZUKD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.016"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..2177)
                     /locus_tag="ACG8N9_000657"
     CDS             complement(42..2177)
                     /locus_tag="ACG8N9_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4896283.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2260..3915)
                     /locus_tag="ACG8N9_000658"
     CDS             complement(2260..3915)
                     /locus_tag="ACG8N9_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4896284.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(3917..4231)
                     /locus_tag="ACG8N9_000659"
     CDS             complement(3917..4231)
                     /locus_tag="ACG8N9_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4896285.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4401..4661
                     /locus_tag="ACG8N9_000660"
     CDS             4401..4661
                     /locus_tag="ACG8N9_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4896286.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            4675..4989
                     /locus_tag="ACG8N9_000661"
     CDS             4675..4989
                     /locus_tag="ACG8N9_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4896287.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5719..6273)
                     /locus_tag="ACG8N9_000662"
     CDS             complement(5719..6273)
                     /locus_tag="ACG8N9_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4896288.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6399..8328)
                     /locus_tag="ACG8N9_000663"
                     /pseudo
     CDS             complement(6399..8328)
                     /locus_tag="ACG8N9_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(8634..9350)
                     /locus_tag="ACG8N9_000664"
     CDS             complement(8634..9350)
                     /locus_tag="ACG8N9_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4896289.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9351..11174)
                     /gene="glpO"
                     /locus_tag="ACG8N9_000665"
     CDS             complement(9351..11174)
                     /gene="glpO"
                     /locus_tag="ACG8N9_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4896290.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11188..12684)
                     /gene="glpK"
                     /locus_tag="ACG8N9_000666"
     CDS             complement(11188..12684)
                     /gene="glpK"
                     /locus_tag="ACG8N9_000666"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4896291.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(12820..14280)
                     /locus_tag="ACG8N9_000667"
     CDS             complement(12820..14280)
                     /locus_tag="ACG8N9_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896292.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14270..15565)
                     /locus_tag="ACG8N9_000668"
     CDS             complement(14270..15565)
                     /locus_tag="ACG8N9_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4896293.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(15897..16574)
                     /locus_tag="ACG8N9_000669"
     CDS             complement(15897..16574)
                     /locus_tag="ACG8N9_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4896294.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16604..17695)
                     /locus_tag="ACG8N9_000670"
     CDS             complement(16604..17695)
                     /locus_tag="ACG8N9_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4896295.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(17796..18230)
                     /locus_tag="ACG8N9_000671"
     CDS             complement(17796..18230)
                     /locus_tag="ACG8N9_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4896296.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(18814..20271)
                     /locus_tag="ACG8N9_000672"
     CDS             complement(18814..20271)
                     /locus_tag="ACG8N9_000672"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4896297.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20268..22163)
                     /locus_tag="ACG8N9_000673"
     CDS             complement(20268..22163)
                     /locus_tag="ACG8N9_000673"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4896298.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22221..23996)
                     /locus_tag="ACG8N9_000674"
     CDS             complement(22221..23996)
                     /locus_tag="ACG8N9_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4896299.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24046..25542)
                     /locus_tag="ACG8N9_000675"
     CDS             complement(24046..25542)
                     /locus_tag="ACG8N9_000675"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4896300.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25736..27358)
                     /locus_tag="ACG8N9_000676"
     CDS             complement(25736..27358)
                     /locus_tag="ACG8N9_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4896301.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(27686..28999)
                     /locus_tag="ACG8N9_000677"
     CDS             complement(27686..28999)
                     /locus_tag="ACG8N9_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4896302.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29244..29468)
                     /locus_tag="ACG8N9_000678"
     CDS             complement(29244..29468)
                     /locus_tag="ACG8N9_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896303.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29488..29910)
                     /locus_tag="ACG8N9_000679"
     CDS             complement(29488..29910)
                     /locus_tag="ACG8N9_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4896304.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30052..30762)
                     /locus_tag="ACG8N9_000680"
     CDS             complement(30052..30762)
                     /locus_tag="ACG8N9_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4896305.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(30780..31277)
                     /locus_tag="ACG8N9_000681"
     CDS             complement(30780..31277)
                     /locus_tag="ACG8N9_000681"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896306.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31314..31766)
                     /locus_tag="ACG8N9_000682"
     CDS             complement(31314..31766)
                     /locus_tag="ACG8N9_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4896307.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(31901..32506)
                     /locus_tag="ACG8N9_000683"
     CDS             complement(31901..32506)
                     /locus_tag="ACG8N9_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4896308.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(32956..33576)
                     /locus_tag="ACG8N9_000684"
     CDS             complement(32956..33576)
                     /locus_tag="ACG8N9_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4896309.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33578..34447)
                     /locus_tag="ACG8N9_000685"
     CDS             complement(33578..34447)
                     /locus_tag="ACG8N9_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4896310.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34470..34877)
                     /locus_tag="ACG8N9_000686"
     CDS             complement(34470..34877)
                     /locus_tag="ACG8N9_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4896311.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(34896..36185)
                     /locus_tag="ACG8N9_000687"
     CDS             complement(34896..36185)
                     /locus_tag="ACG8N9_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4896312.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        10250 a         7772 c         6055 g        12111 t
ORIGIN      
        1 gttcttttta tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat
       61 acgacaatgc ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc
      121 tcatctccat cgttttcaaa tggatataag attacatgat caaattccag tccttttgct
      181 agactcaaag gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct
      241 tgtagcgttt gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga
      301 gaaaccaaaa tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat
      361 tcgggttctt ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca
      421 taggttttga aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac
      481 cgatggatcg gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa
      541 gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat
      601 tcgatcaata gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag
      661 cgaaatgctg gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact
      721 gcttcatcaa cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa
      781 attctttcaa acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt
      841 tttcgatatt ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca
      901 tcagaaatca atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga
      961 ttctgcacag caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt
     1021 gcctttttcg ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat
     1081 attttttcga tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata
     1141 aaattcggat gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg
     1201 ctacgcaaac gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc
     1261 tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc
     1321 agtggatttc tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga
     1381 ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga
     1441 tacgcaatac gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag
     1501 atattttgac ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg
     1561 atagccttca tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga
     1621 atagctacag acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt
     1681 ctttctttaa taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc
     1741 atgttattgt aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct
     1801 gcatcatctg tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga
     1861 aagtctacaa cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc
     1921 tgattttctg cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca
     1981 atcatcgaaa aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca
     2041 gccatttctt tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt
     2101 tcgttttttt tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt
     2161 ttttgtgtgt tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt
     2221 acctctttta agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag
     2281 ttcttcaggt cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta
     2341 gccagactgc ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac
     2401 gatatattct tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat
     2461 atgtttggct tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa
     2521 aatttttcct tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc
     2581 ttctgcaatg ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa
     2641 tggcggtgca tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat
     2701 tgctgttgct aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc
     2761 ataaatctcg ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg
     2821 ataatagctt gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc
     2881 tttggctgtt ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat
     2941 tgctgttcca ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg
     3001 tctaccttga cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg
     3061 ctgttttaca acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg
     3121 ttcgtctact aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg
     3181 gttatcagga cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc
     3241 atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac
     3301 ttctactcct tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt
     3361 tggaagaata tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt
     3421 ttctgccatt tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt
     3481 ttctaatgca tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc
     3541 ggcttctgtt atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa
     3601 tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc
     3661 gggaaagatg gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa
     3721 tgattctggc gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata
     3781 aggcaagccg caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc
     3841 atcttccatt aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac
     3901 aataacgatt ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac
     3961 cacgtacatt cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc
     4021 tcatccctga ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta
     4081 tactttgtaa tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg
     4141 ttctaacatc aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta
     4201 tcgattttat tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa
     4261 tacctcatgg ggtatattaa acaggagctt tattaatatg tcaaccagaa tgactcttca
     4321 tttttttcac tactttttat atttgcataa aataccgata aggggtatta tctaattata
     4381 aaaatagagg agaatgtgtt atggagattg ataataaaaa tatcctgaac cgtttgaaac
     4441 gtacagaggg acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg
     4501 atgtcatcac tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg
     4561 ttgcagataa tctgaaaaat tgctttgaga aacctgatag taatccagaa gaacaagcgg
     4621 ctaaactaga acaagcgatc aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa
     4681 gtcgttttcc atattgatga attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac
     4741 ctgataaaag cttctccaga aacgacgatt gtcgtatctg taaatggcat cgcgattacc
     4801 ggttatctag actctgccaa tgcagaattc ttagatttgc aaggagtcgt ttttcacgct
     4861 tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt ctttacctga acaagtaatc
     4921 gttgtaccag caggtgtcct tgatttagtt gaactgcaat cccaaggtta tgcttatatc
     4981 aagccttagt cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt
     5041 tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca
     5101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt ggttgggctt
     5161 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt
     5221 tgacctaagt attagagaaa aaaacgggaa aatagctcct catattttgc cgttttttga
     5281 gctaataccg aaggtcagct gattgaacac cgtttaagga agagattgtg aaaaagtcgc
     5341 ttagtttcga gtattaagaa aaattttgtg aaaatggatt tctacatttt tgcataaatt
     5401 tggcttaata ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag
     5461 cggtttgacc tgagtattag agaaacaaac ggaaaaatag ctgctcatat tttgccgttt
     5521 tttgagctaa taccgaaggt cagctgattg aacaccgttt accaagaggt tgtgagccta
     5581 ccatttaact ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt ttttggttcg
     5641 gttggcttat tatcgagaag tagctactgg gagtctattt atcaaaaaaa ggaatgcgac
     5701 ataggtcaca cccctttttt attttctttg aaacatcttt cggtactcga cagcatacaa
     5761 gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa atgactctca cccacagcgt
     5821 tggtaattct gggaaaagca gcaaaaggat catgccaaca tagatgacag cagtcgctac
     5881 tttgccatac cacttcgctc cacttaattt gatattttta gatagcccga ttaatccagc
     5941 tacaaacata aagccttctt ttatcaagaa tagaccaaac aacagccaaa gccacggtcg
     6001 ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag
     6061 caccttccct aagtctgtta cctgattata tctccgagca atgaccccgt ctagataatc
     6121 agacaaacct gagacaacaa tgaccgctcc tgctatataa aatgactgtc tcagcacaaa
     6181 ataaagataa atcggcagca aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg
     6241 tatttcttta cgccagtctg tgcttaattt cattttatcg ccctacctcc tcagcactct
     6301 tttcttttta ttttagctta cacggggaat aaaaccaatg atttcactgt ttttattttt
     6361 ctttacagac actgatatta tgagctaatt tgagataatc acttgctgtt ttcatcgttt
     6421 actcttcttt cttcctgtcg tttgcgcatt tcttttacta gtactttatg agcttttttt
     6481 gatcctcgct ctaggtcacg ctgccgttta cgtgttttat aatcgataaa cagactgtct
     6541 aaatcgtaac ctgtcgggta cagatcttct gctgaacgca ccagacgaac acgctgacga
     6601 ggcacgtgga ttattttttt gtctaaatat acatcaatcg tatcttcacc tatatcttca
     6661 taaactaacg cttctttttg gtattcattg acaaatatcc gatcaccttt tgcaaagagc
     6721 tgacgttctt ttttattctc cttcctattt gtaactgctg tcttaggaag aaattctttt
     6781 ttctgtgtcc gataggcttt ttccgccaag taacgctctg cttgatggat caatttcatc
     6841 gacatatcca ttttatgagc aatccacaaa gcttggcttt ctcctgtttc ttccaacccg
     6901 caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc
     6961 ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc
     7021 cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc
     7081 tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat
     7141 agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg
     7201 gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg
     7261 aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt
     7321 tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac
     7381 atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg
     7441 ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc
     7501 aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc
     7561 tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt
     7621 taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt
     7681 tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat
     7741 aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt
     7801 tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa
     7861 ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat
     7921 tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa
     7981 caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct
     8041 aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg
     8101 ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa
     8161 gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga
     8221 gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc
     8281 ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt
     8341 tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa
     8401 cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac
     8461 aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat
     8521 tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac
     8581 ttaactatag caagacacat tttggttgtc aaactttagt atgttttata gatttacacc
     8641 attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc
     8701 catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat
     8761 cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga
     8821 ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt
     8881 tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca
     8941 tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca
     9001 tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa
     9061 gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc
     9121 atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata
     9181 tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc
     9241 tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg
     9301 atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat ttatttctcc
     9361 ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc
     9421 gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg
     9481 atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg
     9541 cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga
     9601 ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc
     9661 ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct
     9721 tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc
     9781 tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg
     9841 cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat
     9901 aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt
     9961 gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa
    10021 actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa
    10081 atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga
    10141 agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga
    10201 ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct
    10261 ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa
    10321 accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt
    10381 ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc
    10441 gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt
    10501 gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga
    10561 tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg
    10621 gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag
    10681 acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct
    10741 ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg
    10801 tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt
    10861 ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt
    10921 tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca
    10981 tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat
    11041 cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca
    11101 ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc
    11161 ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt
    11221 tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt
    11281 gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc
    11341 tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac
    11401 attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac
    11461 tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac
    11521 ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt
    11581 caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa
    11641 tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc
    11701 gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac
    11761 aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt
    11821 caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc
    11881 tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc
    11941 ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg
    12001 cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt
    12061 tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg
    12121 actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca
    12181 agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac
    12241 atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt
    12301 tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga
    12361 ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat
    12421 tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc
    12481 tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc
    12541 atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat
    12601 gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc
    12661 catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta
    12721 actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt
    12781 ctttttttga cagtttttta ttatgaagct attttttaat caattctttt cttttttcag
    12841 cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata
    12901 atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc
    12961 ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga tcagatattg
    13021 ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt
    13081 tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag
    13141 ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc
    13201 cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg
    13261 ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg
    13321 gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat
    13381 cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc
    13441 atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat
    13501 cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg
    13561 atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc
    13621 gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg
    13681 tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg
    13741 tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa
    13801 tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat
    13861 ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga
    13921 ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa
    13981 atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa ttttttccaa
    14041 acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt
    14101 tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt
    14161 tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag
    14221 gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat
    14281 aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa
    14341 atagctcatg ttcttctaac tgttcaaacg tccatccttc ttctactgca attgtcagtt
    14401 ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt
    14461 cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt
    14521 ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta
    14581 cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct
    14641 ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta
    14701 ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa
    14761 tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat
    14821 cattcaatgt taattgttct tcccacatct gattgtcggg acgagaatga ttagccagca
    14881 aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg
    14941 tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta
    15001 ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt
    15061 aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc
    15121 ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt
    15181 tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt
    15241 gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa
    15301 cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc
    15361 ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt
    15421 ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat
    15481 atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg
    15541 acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct
    15601 aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg
    15661 atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta
    15721 ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta
    15781 taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga
    15841 aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa
    15901 ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct
    15961 gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt
    16021 gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt
    16081 gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca
    16141 gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct
    16201 tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac
    16261 attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca
    16321 ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa
    16381 tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga
    16441 ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca
    16501 cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg
    16561 atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt
    16621 tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgctttt tcaaaaggtt
    16681 tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga
    16741 tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc
    16801 gattgcggat cccaccgaca ccaactaaag gaatctttcc ttgaatcgtg ttatggacca
    16861 cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta
    16921 atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt
    16981 cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga
    17041 atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa
    17101 aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag
    17161 agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg
    17221 attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt
    17281 cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata
    17341 caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa
    17401 taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca
    17461 tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg
    17521 cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat
    17581 cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc
    17641 ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca
    17701 ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta aaccactcga
    17761 ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt
    17821 gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat
    17881 gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct
    17941 gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct
    18001 aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct
    18061 ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc
    18121 agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc
    18181 acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct
    18241 ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt
    18301 ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg
    18361 ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt
    18421 tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
    18481 taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc
    18541 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga
    18601 gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt
    18661 gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt
    18721 gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac
    18781 aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat
    18841 gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc
    18901 gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct
    18961 gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat ctttgatagc
    19021 ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt
    19081 tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt
    19141 ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca
    19201 gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg
    19261 gaccccttcc caattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga
    19321 aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt
    19381 gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac
    19441 atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc
    19501 acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat
    19561 aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg
    19621 gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg
    19681 gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt tatttttata
    19741 gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca
    19801 tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg
    19861 ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc
    19921 attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata
    19981 gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc
    20041 atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg
    20101 ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa
    20161 tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg
    20221 attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc
    20281 cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg
    20341 aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat
    20401 tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact
    20461 gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc
    20521 tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact
    20581 tccccgttta cagaactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct
    20641 aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct
    20701 gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg
    20761 aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa
    20821 acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa
    20881 gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg
    20941 taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat
    21001 aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa
    21061 aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc
    21121 gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac
    21181 ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta
    21241 aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga
    21301 cctacaacta ataaaataat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa
    21361 tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat
    21421 acagctaaaa ttgctggaat aaccgtactt gtataccttg ctagaatcac gggcatccct
    21481 agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct
    21541 gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc
    21601 aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt
    21661 cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa
    21721 ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa
    21781 gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt
    21841 ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac tacgacttga
    21901 aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta
    21961 tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg
    22021 ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc
    22081 atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag
    22141 ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa cgaaactgta
    22201 caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt
    22261 atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga
    22321 tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata
    22381 agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag
    22441 attagttgct gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag
    22501 taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa
    22561 atctgataaa tctcccaaga ttcaaactca tctagaacac taggtagata aatcaaatca
    22621 tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg
    22681 tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc
    22741 atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca
    22801 atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa
    22861 taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt
    22921 aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta
    22981 tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca
    23041 tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt
    23101 agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat
    23161 agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat
    23221 aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct
    23281 aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc
    23341 tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc
    23401 ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa accttgcgtg ttggatcacc
    23461 gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt
    23521 agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa
    23581 atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt
    23641 tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa
    23701 tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca
    23761 gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc
    23821 tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa
    23881 gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac tacaagaatt
    23941 tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag
    24001 gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga
    24061 tctatattca aggtttttac cattagactg gataatttgt ttataatatt caaaactatc
    24121 ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc
    24181 atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa
    24241 gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt
    24301 tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag
    24361 tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat
    24421 gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt
    24481 caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt
    24541 tttatagaca gcgctacgat aataactaaa tgcaatataa tctgccgtgt tagcttgcaa
    24601 gcactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc atgtcctgta
    24661 aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt
    24721 aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc
    24781 agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta ataattgagt
    24841 agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatcactt tttttgttag
    24901 tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata
    24961 aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg
    25021 gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg
    25081 ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac
    25141 tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc
    25201 ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg
    25261 aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa
    25321 atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt
    25381 tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt
    25441 cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc
    25501 tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta
    25561 tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt
    25621 cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt atatataaca
    25681 aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa acgatttatt
    25741 ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt
    25801 tcagaggata atttgacaca ataaccctta aagcttcacg tggaatagtg aacgtctgct
    25861 tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg cgagaatagg
    25921 caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg atgatgtcat
    25981 gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct tgatctgcta
    26041 acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat ggaattccag
    26101 tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca ttgtctcttc
    26161 ctttcgcctt gattgattcc aagatttcct cttttgtaaa tccagaagaa atcctttcgt
    26221 tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt gggcaattcg
    26281 tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata tggagcaaga
    26341 tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa cgagaaacaa
    26401 ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca cctaaagatg
    26461 tttgccattt ggataatact tttttcagtt cagagatact caagggtttc aaatcccatt
    26521 tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc atggataatt
    26581 catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc catgtttcgc
    26641 caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga agatactcat
    26701 gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc atatcgatca
    26761 cgtccatacg aaagccccca atacctttat cgatccagaa attcatcatt tgataaatct
    26821 cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta tacaagtgca
    26881 gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat attgagcgca
    26941 atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc cgataaggat
    27001 tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta tgattcacca
    27061 ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt tcttccattt
    27121 cttccatcgt accgaattct gccatgattg cttgataatt actgatatca tacccattgt
    27181 catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct aaatacgcca
    27241 gataatccaa cctggaaata atcccccgca aatctccaat accatcttga tttgaatctt
    27301 gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt tgttccatga
    27361 ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt agtctctctc
    27421 actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa cagaataaaa
    27481 caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat ttatgcaggg
    27541 aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc ccttggtatt
    27601 agctcaaaaa acggcaaaat ataagaaact attttttcgt ttttttctct aatactcagg
    27661 ccagaacgct gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccacaa
    27721 atacaaagaa cagaccatac atgattggag atacttcttt tcctctttta gctgctaaca
    27781 tggtaattgg atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag
    27841 gcatgccgat caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt
    27901 gtcgcaaaga ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg
    27961 aagtaacaac tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag
    28021 taatagctgt gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg
    28081 caccaacagg agaagtacca aataaggaac ccgcaagcat agctgttgaa tcagaagcta
    28141 aagctttccc tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc
    28201 caactaaagt tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc
    28261 acatttgaag agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca
    28321 aacttggtgc tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta
    28381 aaatcgttgt agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga
    28441 tagcagttac gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta
    28501 atcctactaa tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag
    28561 cgataaatag accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa
    28621 tgatcatttc ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata
    28681 cgccggctaa tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa
    28741 aagcattgat cccaagagca ggtgccgttc caattggata acgggctaat acccccatta
    28801 aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc
    28861 cagaagcacc taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg
    28921 taaatcctgc cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg
    28981 aaagtatttt ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt
    29041 taaaatttcg atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag
    29101 taaacaaaaa attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta
    29161 tctcaaagtt catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag
    29221 tttcttcatt ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt
    29281 tcttctttga tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct
    29341 gtatcgacca tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct
    29401 cgaagcttct cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt
    29461 agatccactg tttttcctcc tttttattca atcagacgac tcagcttttc ctataacaaa
    29521 ataattgcct tcagggtcag aaaaattgaa agtcaagatg ccattgttat ctgtgatttc
    29581 tcctggttct tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga
    29641 aggcggtggt cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc
    29701 taattctaat tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat
    29761 tgtttcagaa atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc
    29821 attgacataa agcatcacac gtgttttttg tatcatcatt tgacccttct ttctatttga
    29881 ttttctttcc tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt
    29941 tgatactatt caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt
    30001 ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc
    30061 tgcttctgat tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc
    30121 gattttttcc ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat
    30181 ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc
    30241 acgccagttg agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg
    30301 aagtgccgga gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt
    30361 tcctaaatgc agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa
    30421 agacaaggaa atactattat agatatctac aagcggattg attgggaaaa attcccgttc
    30481 ttgatttgca cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa
    30541 tgtggtaaat gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt
    30601 aaggtaattt cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact
    30661 ttcttttgtt acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg
    30721 aaatacttcc cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat
    30781 tagaataatt gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca
    30841 tgttcgctgt atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt
    30901 tcccaaacac cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta
    30961 tctagtaaaa atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg
    31021 atttctaaaa aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg
    31081 taacccgcag gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc
    31141 tcttctgtaa gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt
    31201 gaattatctt ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct
    31261 acatctgaag gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag
    31321 atccttttat tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta
    31381 tttaaaagat attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca
    31441 ttggagtagt tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga
    31501 tagagtttct tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat
    31561 tctaatttct tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt
    31621 ttttcttgga tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt
    31681 gttaaatcga cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt
    31741 ccgatgtctc gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt
    31801 tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat
    31861 aactagtaaa gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt
    31921 ttttgttttc taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca
    31981 actcttctat actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct
    32041 cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat
    32101 ctttttgttt attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg
    32161 tttcttcata gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc
    32221 caccaggaag tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt
    32281 ttttgatata ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga
    32341 gcgcttttat ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg
    32401 tccggatctc ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt
    32461 gagaaagtgc gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt
    32521 ttttagcaaa tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata
    32581 aatataatga aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt
    32641 cgacttctct ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc
    32701 ccaccaaacg aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag
    32761 gacagctttt tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa
    32821 gtcacaatat tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga
    32881 gctgaacgac aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat
    32941 taagccattt ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg
    33001 agtgtaatcc acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa
    33061 tttgtcatat tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt
    33121 ccctttcgtt cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa
    33181 atgacgatct gattcacgat cacgtttgta ccgacgttgc cataacactt caaagtctgc
    33241 tgttaggcga atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc
    33301 tttttgctta tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata
    33361 caaatcaagt gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc
    33421 ttccaaattg ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat
    33481 ggcatacata tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc
    33541 aggactacca gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg
    33601 gactttccaa gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat
    33661 ttgctggaat cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt
    33721 tagctacttt cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt
    33781 tattgatcaa ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg
    33841 ggacatcaac tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt
    33901 tgaattgtcc atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta
    33961 caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc
    34021 cttcttctgt accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg
    34081 catattctac tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat
    34141 catacatgac agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg
    34201 cgtggtctaa gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat
    34261 tgactaacac atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg
    34321 gcgaacccat ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat
    34381 taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt tgttacttct ttcaatgttg
    34441 tatacatctt ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt
    34501 gcaatttgtc gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca
    34561 gcacgagcag catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct
    34621 ccgatcgtct gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca
    34681 tacaattcag aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct
    34741 tgggtagtgg aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct
    34801 tctggcgata gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttttttc
    34861 ttaaaaaatc caaacatcat tttccctact ttcaattaaa atgctttcaa aactgggctt
    34921 aagatccagg catataagag tgctgcggct actgtatcta catcggttgc tgtagcattg
    34981 ttgaatccca tcgaattaaa gatcgttact agcaaagcgg gcaataatgt aataaagaaa
    35041 ccatgagcaa taccgccaat gatcgctcct cggcgtccgc caactgcgtt gccaaatatc
    35101 cctgctgtcc ctccggcaaa gaaattggtt agcataccag gtaaaatcat cgctaagccg
    35161 aacatgggca aagtaaacat ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa
    35221 atgacagcat ttgggctata aggaaagaaa actggacaat ctaaggcagg gatcgcatca
    35281 ggaactaatt tcatagctat ccctcgaaat gccggcacga tttcacctaa cagtagacgt
    35341 acaccagcta gcaatacata cacacccact acgaattgga tggcttgtaa aaaggcaaac
    35401 atgatatagt tttgatctcc tgataattca gaagcgaatg gttcgcctgc aaataaaaca
    35461 gtgatgatat atagcgggac catcacgacc atgacagaca aataagtatc ttgcagaaat
    35521 tcgaaatgtg tcggcaactt gatatcttcg atcgactttt tattttcgcc tttctcacca
    35581 aaaagcttag caacaccggc ttcaaataag tacccgatcg tacaaaaatg acctaaagca
    35641 atatcatttg atccagtgat tttacgaatg atcggttgcg ctactgccgg catagcaatg
    35701 gcgaaacaag ctccgataaa accaccgacc aagatcaata cgatccctcg cagaccggca
    35761 aaatagccaa agactgtcgt catcgttgcc atccataaaa ttgcttgacc agttaaaaaa
    35821 atgtacttcc attttgtgaa acgagccaaa ataatattga atacaaaaat ggctaagaac
    35881 gtcagtgcaa tatctctacc taaacccaaa tcattcattg cttgaccatt gatagattca
    35941 atagaaggga tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt
    36001 aaacttccaa cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt
    36061 aacgtgccag agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca
    36121 atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg
    36181 cccatgtt
//
LOCUS       DAZUKD010000017        35506 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000017 DAZUKD010000000
VERSION     DAZUKD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.017"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            307..627
                     /locus_tag="ACG8N9_000688"
     CDS             307..627
                     /locus_tag="ACG8N9_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4896313.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            781..1686
                     /locus_tag="ACG8N9_000689"
     CDS             781..1686
                     /locus_tag="ACG8N9_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896314.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1683..2456
                     /locus_tag="ACG8N9_000690"
     CDS             1683..2456
                     /locus_tag="ACG8N9_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4896315.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2637..4061)
                     /locus_tag="ACG8N9_000691"
     CDS             complement(2637..4061)
                     /locus_tag="ACG8N9_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4896316.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4272..4718
                     /gene="nrdI"
                     /locus_tag="ACG8N9_000692"
     CDS             4272..4718
                     /gene="nrdI"
                     /locus_tag="ACG8N9_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4896317.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4783..6765)
                     /locus_tag="ACG8N9_000693"
     CDS             complement(4783..6765)
                     /locus_tag="ACG8N9_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4896318.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6779..7675)
                     /gene="murQ"
                     /locus_tag="ACG8N9_000694"
     CDS             complement(6779..7675)
                     /gene="murQ"
                     /locus_tag="ACG8N9_000694"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4896319.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7678..8739)
                     /locus_tag="ACG8N9_000695"
     CDS             complement(7678..8739)
                     /locus_tag="ACG8N9_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4896320.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8950..9477)
                     /locus_tag="ACG8N9_000696"
     CDS             complement(8950..9477)
                     /locus_tag="ACG8N9_000696"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4896321.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9544..11046)
                     /gene="eat(A)"
                     /locus_tag="ACG8N9_000697"
     CDS             complement(9544..11046)
                     /gene="eat(A)"
                     /locus_tag="ACG8N9_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4896322.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11304..12424)
                     /locus_tag="ACG8N9_000698"
                     /pseudo
     CDS             complement(11304..12424)
                     /locus_tag="ACG8N9_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            12550..13491
                     /locus_tag="ACG8N9_000699"
     CDS             12550..13491
                     /locus_tag="ACG8N9_000699"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4896323.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13641..14537)
                     /locus_tag="ACG8N9_000700"
     CDS             complement(13641..14537)
                     /locus_tag="ACG8N9_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4896324.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14806..16146
                     /locus_tag="ACG8N9_000701"
     CDS             14806..16146
                     /locus_tag="ACG8N9_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4896325.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16364..16807
                     /locus_tag="ACG8N9_000702"
     CDS             16364..16807
                     /locus_tag="ACG8N9_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4896326.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17311..18729)
                     /gene="nhaC"
                     /locus_tag="ACG8N9_000703"
     CDS             complement(17311..18729)
                     /gene="nhaC"
                     /locus_tag="ACG8N9_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4896327.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19132..20355)
                     /locus_tag="ACG8N9_000704"
     CDS             complement(19132..20355)
                     /locus_tag="ACG8N9_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4896328.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20386..21225)
                     /locus_tag="ACG8N9_000705"
     CDS             complement(20386..21225)
                     /locus_tag="ACG8N9_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4896329.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21242..21922)
                     /locus_tag="ACG8N9_000706"
     CDS             complement(21242..21922)
                     /locus_tag="ACG8N9_000706"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4896330.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21924..22997)
                     /locus_tag="ACG8N9_000707"
     CDS             complement(21924..22997)
                     /locus_tag="ACG8N9_000707"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4896331.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23053..23291)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23334..23567)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23644..24315)
                     /locus_tag="ACG8N9_000708"
     CDS             complement(23644..24315)
                     /locus_tag="ACG8N9_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4896332.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24402..27050)
                     /gene="mgtA"
                     /locus_tag="ACG8N9_000709"
     CDS             complement(24402..27050)
                     /gene="mgtA"
                     /locus_tag="ACG8N9_000709"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4896333.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27392..27566)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27739..28176
                     /gene="ndk"
                     /locus_tag="ACG8N9_000710"
     CDS             27739..28176
                     /gene="ndk"
                     /locus_tag="ACG8N9_000710"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4896334.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28198..29064)
                     /locus_tag="ACG8N9_000711"
     CDS             complement(28198..29064)
                     /locus_tag="ACG8N9_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4896335.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29224..31317)
                     /locus_tag="ACG8N9_000712"
     CDS             complement(29224..31317)
                     /locus_tag="ACG8N9_000712"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4896336.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32066..32689)
                     /locus_tag="ACG8N9_000713"
     CDS             complement(32066..32689)
                     /locus_tag="ACG8N9_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896337.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32848..34245)
                     /locus_tag="ACG8N9_000714"
     CDS             complement(32848..34245)
                     /locus_tag="ACG8N9_000714"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4896338.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34277..35248)
                     /locus_tag="ACG8N9_000715"
     CDS             complement(34277..35248)
                     /locus_tag="ACG8N9_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4896339.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT        10209 a         7440 c         6228 g        11629 t
ORIGIN      
        1 gcccgataat atatagaaat aaggtctagt tactgttttt tttaaatgat taataaataa
       61 caacttcttt ctatgaaatt gcaaagcatt catcttcttt tccgctaacg atatatccgt
      121 cagtgcttgg tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta
      181 tactacagaa aaagatatac aatctgccac cgggtaggat aaagaggatg atcattccat
      241 taggtctttg aagggatgat tttcctcttt ttgtgtattt ttttatagtt aaggaggaat
      301 gtaagtatgg catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg
      361 atgatgaaat ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattggc
      421 atgattgcta gtttttactt tttgtcaaag gcaatcaaaa gtttgccgat gagtctctct
      481 tatcctatct ggacagggat tggcgcagtt ggttcggtca taattggtgt ggtcttgttt
      541 catgataagc tgaatctttc cacttggttc tttgtgggga tgctactcat cagtatcatt
      601 ggcatcaaga ttactagcgg ccactagaca cttggaataa gtactgatca gaaaaaataa
      661 gcctttttat ttaatattca atttttttat gaaaaaataa gaaataagtc caatgaccta
      721 gcgacttctc cactcatcag gtgtatgctt gtactatcta tagaaaagga gtgaatattt
      781 ttgaaaagtc tttcggtcca agaattatcc aagaactttg gtcacaaaca agccctcagc
      841 gatgtcagtt ttagcttatc ccctggcaaa atcgttggat tagtaggtcc taatggcgct
      901 ggaaaaacaa cgatcatgaa agctatttta ggactgatcc attactcaga tggaacaatc
      961 tgtattggtg aaaatgagat ttctccctct agtcataaag ggttggaaca agtaggagca
     1021 ttgatcgaat acccgggtat ctatccattc ttaacaggct atgatcatct gaagttattc
     1081 tctgaaacca acgatgtatc tgctatcgac actatcgtga atcagctcaa gatggaaaag
     1141 tacattcaca aaaaagcgaa atcctactcg ttgggaatga aacaaaaatt gggaattgct
     1201 cttgccttac tgaaccatcc gtctatcgtt attttagatg agccgatgaa cggtttggat
     1261 ccacaagcaa caagagatgt gcgaaaaatc attaccgact tggctactca gggcacctct
     1321 ttcttgatct ccagtcactt actcagcgag cttgaaaaaa tggtcgatga gctgatcctg
     1381 atcaatcaag gaaagatcat caaacaatgt acaatggctg aattgaacca aacagcgcaa
     1441 gatttcatcg ttatctctac aacaaatgat ctagctgctc gaacgatctt gacagaagct
     1501 ggatatactt tggaagatcc tgataaaatc aagcttttaa aagaaaacga agaattatta
     1561 ggaaccgtga tccagctatt gtctgcacaa aatatccatg tgacagacgt ccagcatctg
     1621 acgaatgatt tggaatcttc tctattggaa attctggatt cagtaaaggg ggaagaagaa
     1681 aaatgacatt gcttgtaaaa caagaattat ttaaattgat caagaaaaag tcaactgctg
     1741 ttttatctgt tctattagtt gttttgctca taggaacagc acttctagcc aaaaaataca
     1801 cgacgatcat tgatccagtt gaaatgactg ctcagctttt tagtgccaca tcttggattg
     1861 tctttatcat gattgcagct gcaagcacga tcatttcgat ggaagcacaa tatggtacat
     1921 tgaaaaatct cctttatcga aagtattctc gaggagaaat attagtaagc aaatggatca
     1981 cattagttat ttattctgtt tatttatacc ttttagctat tattgtcact gtgttgatga
     2041 aactgattct tttcccttcg atttccttca cggagaaggt ttctactggt caaacactga
     2101 tccaaagctt gttcacttat actttgggta gttatatcgg tttatggctg attttaagtt
     2161 tagtgttgat gattgcttgc tttatcaata gttccggcgc atccatctca gcaggaatcg
     2221 tcttttattt tgcttcgtca atcatatctg gtatccttat tgcactcatc caaaaatggg
     2281 agtggatcaa atggaatccg atcagtatgt tgaatctgca aaaccaaatt ggaaacgaag
     2341 agatcatgaa gcaattgact catctttcta ctaatcaaat gctattcggt aatttagctt
     2401 acatcgtttt gttcttagca ttaggatatc tcgtatttaa gcgaaaaaat gtatgatgac
     2461 tcatcaaaaa agaggctgtg aaagagacgt tcagccttat tgccgattgg ctgtctctta
     2521 aacaccgttt attctgaaaa aggagctgtg acaacatttg tgtcacagct cctttcctag
     2581 tatgtttttg aataaacgac aggctcacaa cctctcttta tacttctttt atacttctat
     2641 acgtgaagca tcccagtggc aaataaaacc attgcaatca ctccgaccat cgtcacacct
     2701 ataagtaaaa tcttatctaa gtggatatgt tgtacaggct cgttttgctc cttacgtgct
     2761 ttgaggtaaa aataaatgcc tggcaagaaa agaacaagaa caagcattac ttcattaaag
     2821 gctacgacta gcatagccca tacttgaaag gcacttgcta aaagccctac taaaaattgg
     2881 acccattctt tccgctgata agaaagtttc atttggtaca atcctaagaa aagccacgtg
     2941 aagaaaatag ctgctgtacc aagtgtgtaa gcaaagttat aagcactgtc agtgaataat
     3001 aatgaaagta aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc
     3061 ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc cgtcttcagc catgtttccc
     3121 attgtttcaa tcggtaagat cgtccatgag agccaaacac caactaaaga aatgatcaaa
     3181 ccgccgttga tcacgactgc tccccattta ccaaccatca tttcaaacac atagcccata
     3241 gcaggctgcg ccttgatcgt ttgcagctga ttcatcgtca ttgtcccata tggaagcatg
     3301 gaaaccaaga cgtaaataat aatcaagccg aatatcccta gaatcgttgc tttcccaaca
     3361 tctgattgtt tttttgctcg tgaacctacg acagaggctc cttcaatacc aacaaatacc
     3421 caaagcatca ccatgatgca actcaatact tgactggaaa acgggatcgg ctgtccattc
     3481 gcattcaata taatgtcgct agaaaagtta tcaatgaatt gttggatcga aaacgtgctg
     3541 atagctacta caataaataa aaataatggc actaatttac agaatgtgac gatagcattg
     3601 acgaaactag cattttcgac tccccgattc actagccatg catatcccca taaaaagact
     3661 gtactcatca cgatcgatgc cagattttgc ccaccttcaa atactgggaa gaaatatcct
     3721 aatgcgctca tcaacaatgt tgcaaaagct acgttgccta accagcatgt gagccagtaa
     3781 ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc
     3841 ccgcctttta aatctggacg ttttttattg atattattga tcgacaagga gagcatcaac
     3901 attgctgttc ctacgagtac ccaactgaaa atagctgggc cgccagccga tccggagatg
     3961 tccgtcataa taccaaatac gccacctcca atcgctgaac caattactaa ggcagcaagt
     4021 ggaattaaac caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaaata
     4081 aaaccgcttt tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac
     4141 tttaatagaa atataattct accggctttt ttctcattat acaagcaaag cgcctagtcc
     4201 ctttccaaat tacaccacca aaaaacttaa aaaagatgct atactaaaag gaaagaattg
     4261 gaggatgatc catgaacatt ttatatatat cgatttcagg aaatacgcgt gcctttgcca
     4321 aacatctagc agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta
     4381 ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt
     4441 tcgttcctac ttatttagac ggcggaaatg gtttagataa cggtgataca gaaatcttga
     4501 ccgaaacgat gcgtgaatat ctggaacatg aagacaatca taaactttgt cttggtgtag
     4561 tcggtagcgg aaataaaaac ttcaataatc agtattgtct aacagccaaa caatatgcac
     4621 aaacatttgg ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga
     4681 cacgaattta tcagatttta gcagaaaaca ataaataata aaaaagaaga agctgaaaaa
     4741 tagatttcag tttcttcttt cgtttctatt gtttctatca tattaagctt tgacatccat
     4801 cacaggtgct tttgcttttg ctttacctgt tttttccaag acaaagtttt ttacacgatc
     4861 catattggtt accaagacga tcatggatgt ttctttgcct gctgctttga tcgcttctaa
     4921 gtcagcttct gctacaagcg tatcaggtgt cacttgctgc ccttctgtta ctttcacatc
     4981 aaatggcgta ccgcctaagt caactgtgtt gattcccata tgaagcaaga tttctaaacc
     5041 atttggcgtt gtgatcccaa tagcgtgttt cgttgggaag acagagctga ctaccccagt
     5101 aactggagaa tagatatgtc cgttttctgg ttctacaaag tagccttcac ccatcatctt
     5161 ttgtgcaaaa actggatcac ttgctttatc taaagaaaca actgttccgt cagcaactga
     5221 tgagaggata gtcacacttt cagctgaagt ttgaggtact actttttcag gcacttcatt
     5281 tactgtggct gtttcagtcg ctaattcttc tttttctaaa cggtctttag gaataccgaa
     5341 gaagaatgta gcaacgaagc caccagcata agctgccaat aatccaagga tgtagcctaa
     5401 ccatttaccg tcagagatca atgggatcaa agcggctcct gaaggtccaa tagcgatagc
     5461 cccgacattt ccgatcattc cgatcactgc cccgccgata cctccaccga tacaagcagt
     5521 aataaatggg cgtcctagag gtaatgttac tccgtaaatc aatggttcgc cgattcctag
     5581 gatcccgact ggtaaagctc ctttgatcat ttctgttaat tctttatctt ttttgcagcg
     5641 aatccataat gccaatgctg cacccacttg tcctgcacca gccatcgcaa gaattggaag
     5701 caatagcgta cgaccagttt catcaatcat ttgcacatgg attggagtca aaatttgatg
     5761 cagtccgaac ataaccatcg gcaagaataa tgtgcctaga acgaagccgg caaatgctcc
     5821 accaacgcca agtacccagt tgatcccacc gaccatgcca tcagaaatga aacctgctaa
     5881 aggcatgatg aagaaaattt caatcagacc gatggcaatc aatgaaagag ttggtgtaac
     5941 gatgatatct accgcatcag gtactacttt atgcagtttt ttctcaacga ttgacaatag
     6001 ccaaacagcg aagatgacac ctaggatccc accttgtcca gctgcaagag cttcaccagt
     6061 aaataagttt ttcagtggtg cttccggatt catccctgtc agcatcacaa cggccccgat
     6121 aactccacca agagttggtg ttgcaccaaa aacttgtgca gcattgatac ctgtatagat
     6181 aactaggtag ctaaacatcc cgtttttcaa gatgttcatc acatcgatat attgttgcca
     6241 tgttgctgca tcaagtgttc cggctgtgac taagtttgct aaaactgcgg caatccctgc
     6301 cacgatccca gtaccgacaa atgcaggaat cattggcaca aagatattag aaatgtcttt
     6361 taataccgat ttgattttag atggtttttg tttcgatttt tgtgccgctt tcatttcttg
     6421 tgcccgttcg tttacacggt cttttccaga tgctgatgaa tttgctgtag ctgtacttac
     6481 agaagaaagt ttttctccta atttcacgcc tgccaaatca accatttgct gtgctacttt
     6541 gttcactttc ccaggtccga tgatgacttg aagctgttca tcttctacta caccaagcac
     6601 tcctggaatc gcttttaatt cagagacatt tacaagatca tcattgatga tcttcatacg
     6661 cacacgagtc atgcagtgga caatgctttc tacattgccc atgccgcctg cccctttata
     6721 aatttgtttt gccagttctt ccaaagtcag ttcgtttttt gccatggtct tttccccctc
     6781 ataaggtccc ttttacaaat ccgtttgctt cagctaattt tgcttgagct tcctctttat
     6841 cactatttgt caaaatcatg acgatagcca gcttaacatt ttgatctgct tcttcaaagt
     6901 acttttcagc tgtttcgtaa tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt
     6961 cgattagttt ttcatttgtt ggtttgacat cgaccatcaa gttattgaat actttaccta
     7021 taccaatcat actgatagta gaaatcatat tcaagataag tttttgtgct gttcctgact
     7081 tcaatcgagt agagcctgtc aaaaattctg gacctgcatc gacttcgatt gggaaatccg
     7141 cgtgtttact gatttcggct tgtttattgc aagaaatcgt tcctgtgaca gcaccgatcg
     7201 attttgcgta atctaatcca ccgatcacat atggtgtacg accgctagct gcgataccaa
     7261 taactatatc attttttgtc aaattcagct tgattagatc ttcttgccct agctcataag
     7321 aatcttcggc cccttcgaca gctacagtca ttgcttcttc tccaccagca attagaccta
     7381 tgaccatttc cggttctaca ccgaatgtcg gtacgcattc agcagcatct aacacaccta
     7441 gtcttccact tgttccggct cccatataaa tcagacgtcc acctttttta aaggactcaa
     7501 tcgtgttagc aatcactggt tcgatacttg tcagttcttt ttcaaccgca gcggccactt
     7561 tctgatcttc tttgttcatt agttgaacgg cttgagaaat actcatctca tctaatccaa
     7621 aagtttcttg gtttctgcgt tccgttgtta ggttctctaa attgattttg ctcattttta
     7681 tcttccttct tttcttataa attgatagtt cataccagct ttgatttggt tgatcaatgg
     7741 tagctcttct ttgataactt tagctactac attcactttt tcatccgctg gcaaatcata
     7801 tttggttaat tggatctcac ccatatagcg caaatacttt tcgttatcta aagtaataga
     7861 ccctttcata cgcgtagctg tatttcttgc tgggataggc ggaatctctc gaaaacgtgc
     7921 atctacgctt cgtatgacat ctcttgcatc gtcttgccga ttcgtatgct tccctaatat
     7981 atattcgtaa aattcttcat ctacaggttc cgtatggagc aagactgtct gttctttttg
     8041 gaaagaagca aattgctcct gaacgttttc tgacagacct gcatctccga tgtaaacaat
     8101 atccgtgtta cttgctaata gatctagagc gcctgcaaga ggatggatcc ctcgatgttc
     8161 ttccaaagtt ggcaatcctt gatatagtgg gcctctcaac tctgtatctc ctggtacaaa
     8221 accttggaca aggagacctt gaattttgag ccattgattt tttttctgat accattcttt
     8281 gtccagccct gtttcgggac gtggataata attatgccaa gcttcaatct gtgagaaatc
     8341 agcttctgct tctttcaatt catcgatatc tttaggcgtg atcgtgctag cattcaaact
     8401 gattttcaat tgatgtgacc agtcggcaat ctgctgattg gaaatatgat aatccattct
     8461 taaacctgtt acaccaattg ctttcaaagg cgttagatct tctaatgaaa aacccgctcg
     8521 ttccaatgcc tctcctgaaa tatcgaccat caattctagt ttattctttt tcgcttcgct
     8581 tcctagttcg atcaatcgct tttgatatgc cgaaacatca tcttctggta tatgcatgga
     8641 agtgaaaata ccgacaaagc cattggatgc catttggcgg atatactgtt tcttctcttc
     8701 tgacaaagaa tcgttcataa atattgaaaa gccaaacata ttatccctct ttttctgtat
     8761 ccgtcttcat ttctatgact agagtatata tctaaagaaa caaaatttca atacttttat
     8821 taaaataatt aaatttcatt tctaaaggtt gagtttatat tatcaaaaga attgtcataa
     8881 cctaaaaaag gcactgctaa gcaaaagcag tgccttgaaa tattttattt aaataacaca
     8941 aaaacataat tactcataag aaaccaattc tgtattatct accatgaatg taaagaaccc
     9001 ctcattaggc atcggatgaa aataagcatt gatcactctt atcaaggcat tatggcaaac
     9061 taacagatag acattttctt cgtcttcgat gcattctttc aataaaggaa cgatccgtgc
     9121 atacacatct aagactgact ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt
     9181 ttgaaagttc tcatctttgc ttggcagtcc atcataatcc ccaaaatcca tttctaccaa
     9241 tcgttcatca acggtcagcg gcaagctgag acgatcagca actgcttgtg ctgtatctct
     9301 tgctcgttga agaggggaat ggatgatttt cgtgattgga acaggcaaat ctactacctt
     9361 ttctgctagt ttctgtgcct gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc
     9421 gcaaactaat ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg
     9481 attctctcct tcgttggcaa agttcccctt aaccatacct tgttgttctt gagattacaa
     9541 gggttagatt ctctcaagag aaataatctc agtagatact ttatccagga aggtttgatc
     9601 atgttccact agtaacatgg caggtttcac gttcaagatc agttgttcta attgttcttg
     9661 attgaagaca tccaaataat tcaatggttc atcccatgta tatagttcag ctggctgtga
     9721 taaagattta gccaattcca cttttttccg ttggcccata ctcatctgct cgatcttgtt
     9781 atgaaaaaca tctctttcca tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc
     9841 tacttggttt ttctctgcaa attccgccaa cgttcctcga ttgtcttcat aattttggct
     9901 tgcataacta atgctcagat gttttggctg gtatttttct cctataacct tgccgttgaa
     9961 tgcccccaga agataatgga taatggatga ctttcctgca ccgttaggac ctgaaatcgc
    10021 cacccgctga tgaggctcga ttgtaaaatg aattggctcg aataacagat tttcataccc
    10081 taattgaaga tcttctacgc ttaaaagtcg cttatggtga gacgcttggc tattcatcgt
    10141 caaagaatcg atatattcga tatcttttaa tagtttttct ttctcgctga tctgggtctc
    10201 catccgattc acgattgctt tggatcgttt catcgtccgt gcagcatcag cacccactgc
    10261 tcctttattc actcgtctag attcagtatc gatgaatccg acttgtttct ttgttttatc
    10321 tccttctcgg gaacgagacc attcggcttt ttcagctgct gttttcttta gcctactgac
    10381 ttcttttttc aatttttcat tttgagccat ttcaaattca tcacgaagtt ttttctgttc
    10441 ttcatagata gagaaattcc cttgataaag ttccagttga cttttttcga ttgctaaaac
    10501 atggtccact acttcatcga taaatccccg gtcatggctg accacgatga agccttgttt
    10561 tttctttttc aaataagccg ctacttgttt tctaccagag atatccaaat gattcgttgg
    10621 ttcatcgatt aacgggaaat gagtgtcatc cacaaataaa agtgccagta ggacttttgt
    10681 cttctctccc cccgatagtg tgctgaattc tctccataag atttcaggat ctgtttgcat
    10741 caattggagc tctctttcga tttcccatat ctcaaaatcc gtaatatcat ttaacacgta
    10801 ataggttaaa cgttctttat cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg
    10861 gattacttgc ccttgataag gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc
    10921 tcgaccgttt cgtccgataa gtcctagttt ccattgtgtg tcaaaattta gatttgcttg
    10981 ttcaaataat aatgtgcctt ggctgtcgta gccgaatgtc agatttttta tttcgatttt
    11041 agacattttc catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag
    11101 gatgcaaggg ttggatacac acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg
    11161 aaaatctgca cactgcttcc ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa
    11221 tcgacttaat ttttcaatgc acggatccct cactttgtcc tatatttgaa attacgatag
    11281 cataaagaaa atgattttgt caactacttc gggaggatgg tgaaaaatct gctaagtgtc
    11341 tgtcctaaga tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct
    11401 attggaccca aatgaatact taagacaatt gggagaaaat cagaaagtcc ttctagctct
    11461 tggtctctac ttccgcaaat agcgattatt ggttttcgat atttttttgc aagcactgct
    11521 acaccgtatg gaacttttcc gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg
    11581 atgacatctg cttgttcgat ctttttttcc aatccagtga tttggctgac taaatcgaaa
    11641 ccagaaacca tcttcgctcc caacaaaaaa gggctccacc aataccgcct gctgctccac
    11701 ttcctggaat atcatctaaa tccacctccc agaaatcttt tatttgctga gcaatctttc
    11761 ctgcttgttt atctaagaaa gctagcgtcg tatctattcc tcctttttgt cttccaaatt
    11821 gccaagcagc tccttgttga cctgtatatg gattggtaac atctgcagcg ataatcaatt
    11881 ttactttaga aacaagaacg tttcttcgat caaaatgaag ttttttagta gataataaag
    11941 gatttccttc tgtgtaccct tctaaagaag cacccaagga ttggagtaat cctagaccac
    12001 catcgctaca cccgcttcct cctaaagtga caataatttg ttctacttct tgaagcagtg
    12061 catctttaat aagttctcct aaggcataac tggaagcaag tcggatagtt tgatccgttg
    12121 gtttatcaag tagatctaac cccaatgctt gtgcagattc gatgactgcg gtgttttttc
    12181 cttgccaagt tgtcaataaa tagctgattt ttcgctgacg gaataagaga tccatcccat
    12241 caatggtacg ccatgttccc ctgagcgcgt aatagattgc ttctgcagta ttttctccgc
    12301 catctgcaat cggcacattg atttttcttc ctttacgatc ttcccagcca tttagaactg
    12361 cctgatttag ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc gccaaaatat
    12421 ccatctgtat ccctcacttt cttcttaagt aagacacttt ttttcatttt tttcaaacaa
    12481 aacaatgcta ttttcttcca tttttctata tactaaaagg gaactcattg aataaaggag
    12541 agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa tgggcagccg aatcggattg
    12601 atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc tcacattgaa
    12661 gccattcgta caaatgggtt gattgctgac tttaacggta aagaagtggt cgcaaaactg
    12721 ccaatctatt caccagaaga aatcatcgaa agcaacgaac acgtggactt gatcgtagca
    12781 ttaacaaaag ccaatcaatt agatgatatg ttttgttcga tccaatccat tattactgac
    12841 aatacttatg ttttgtgttt actaaatgga ttaggtcatg aagatgtact ggaaaaatat
    12901 gtgccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct agctggtccc
    12961 ggaaaagtca cattgttagg tgatggagaa atcgaactag agaacttgga accagaaggc
    13021 gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa tccgatctat
    13081 agccataacg ttcgttattc catttggcga aaagcttgtg taaatggaac attgaatggt
    13141 ttatgcacga ttttggattg taatattgct gaactaggcg cgcaaaaagc ggcagagagc
    13201 atggttcgaa caatcgtatc tgaatttgct tctatcgcag caaaagaagg aatcatacta
    13261 gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa catcgggttg
    13321 cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga aatcgactac
    13381 atcaatggtg ccatttggcg aaaaggacaa aaatatgata ttgctacacc atattgtgcc
    13441 tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata aaaggctctc
    13501 tgatgagaat cagctgaatc agcagctggt tctctttttt taagcccaat aaaagatcaa
    13561 aaagaaaaca caactctcat gatgccataa gaattgtgct tgtttgtttc agtacataat
    13621 ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg agaggtcata ttcgtattgc
    13681 tcataaatag tggaatcgtt agtgataaat cgttttgccc aattttctgc atcataaaat
    13741 tccaatagct ctgataggat tggcttaaat ggtgtatgca gaaattccgg taattcatct
    13801 aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata atgagcaatc
    13861 gcttgattat ccgtcactag tttcttttga aaatacatgg attcaaatgt ccgcaaagtc
    13921 actccttgct gaccctcccg caacagttcc agaatcccgc gcgattcccc tgtttttttc
    13981 aggtactctt cataagtaag atatccaggt tgttcattcc ctcgatgtgg aagaatgaaa
    14041 tagttggtcg taacatctct ttttataaat tcctgcttat agctttctgc tcgttctttt
    14101 ctcccattat tcgttgcacc aaaaaataga tccgtctttg gttcgctaga agaaaaattc
    14161 atatgtttcg aataaaatga actattatgt ttcagcccaa gttcttttgc ttccaaaaaa
    14221 tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag catcgcctcg
    14281 tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt aaatgggcgc
    14341 acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag ctgataattt
    14401 gccaactgtt gtctagctga tttaatgaat tccgactgta gtaaatcttt tccggaccaa
    14461 acatctaaag agcggatgga aggcaaaaaa taaagtgatt tattttttgc aactagcagt
    14521 acgtcatttt tgttcatctt ttccgcctca ctttctctgt cactttttcc tatgatagcc
    14581 tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa tttaacattt
    14641 gtatgaaaat atgatgaaat aaatcattct tctattaaaa ttcgcctaaa agttttctga
    14701 atagttgtaa ttgtctgaaa atttgttatc attagtcatt gttaaatatt gctatttatt
    14761 cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga agataaaaaa
    14821 ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg agcaatcggt
    14881 gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc tgtattgatt
    14941 gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg aaaaatcgtt
    15001 ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta tctaggtgct
    15061 cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa tatcatcgct
    15121 gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc agcctgggta
    15181 tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt acggttattc
    15241 gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt actgatcatt
    15301 tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc tacccttcag
    15361 caattgactg accatggtgg gtttactcct catggtatga agggaatcat cagttctttg
    15421 cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt aagcgaagca
    15481 gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag aatcatttcc
    15541 ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac tttagcaggc
    15601 acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt tgcagcggat
    15661 atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg tgtgtatgca
    15721 tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag aaaactagct
    15781 gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag tgttctttat
    15841 cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct ggcaggttcc
    15901 ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt tgtactggca
    15961 ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc tgtcttagga
    16021 ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt gactggcctg
    16081 ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt attgacgaat
    16141 gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc tattgaagaa
    16201 aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt ctcatgacct
    16261 cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct ccatgctcat
    16321 gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa agtattggaa
    16381 gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt acttgaatgt
    16441 ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt tgatgatggg
    16501 tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa acaaaaagat
    16561 aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt ttatggtacg
    16621 aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc atcattctat
    16681 cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga
    16741 agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga tcaaagaaat
    16801 ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta tcagcttcaa
    16861 gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa aagctgccct
    16921 gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg tttggttagg
    16981 cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta aaatcagcgt
    17041 tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt gccatttttt
    17101 gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt gaaaaagtcg
    17161 cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt tgtataaatt
    17221 tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg gattgtgaca
    17281 aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg tcttgcttac
    17341 tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg cactgaccat
    17401 cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga gtgtaggaat
    17461 accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt agttgattac
    17521 tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg ctttttcata
    17581 agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac caataaaaat
    17641 atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt tcgtcatctt
    17701 tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca atccacctaa
    17761 tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc ctccgcggct
    17821 aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt cgatgacccc
    17881 agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta ctgctacagc
    17941 gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca gtaacattaa
    18001 aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa catcagtcac
    18061 ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa
    18121 gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt gtttgaacaa
    18181 atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag acatcttatc
    18241 tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta agcctagtgt
    18301 tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg cactccctac
    18361 agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact ggacactgat
    18421 caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc atacagcaat
    18481 caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga tgccattgat
    18541 gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca acatgaccat
    18601 tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa cgccaatcgc
    18661 aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg gtgttccttt
    18721 ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa gttaagatat
    18781 cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa aagacctgtt
    18841 ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat ctgtttttca
    18901 ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc
    18961 ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc gtgaaaagtt
    19021 gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa aatatttttt
    19081 catttttctg aaaattattt ttataacttt cacacacaag tttatttttt actagaaagt
    19141 agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga ttctttctaa
    19201 tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg ctgcaatcgc
    19261 aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg tgtgtccggc
    19321 aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt ggctgacatt
    19381 tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt catctggaac
    19441 acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa cgatgacatc
    19501 atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta tcgcagcacc
    19561 tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat ttcctgcacg
    19621 taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc ctccgtctgt
    19681 aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt tgattccatt
    19741 aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg ctgctgcatg
    19801 ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt ctgtcgtttt
    19861 cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa aaaaaccttc
    19921 tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg gggtgccata
    19981 tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg cggctaatac
    20041 ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta aagcatcgaa
    20101 ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg catcaaaggc
    20161 tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta attgtttaat
    20221 caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt ggatatctag
    20281 agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt ggtttgttga
    20341 gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt ttctcccgaa
    20401 tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt tgactgtgtc
    20461 ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat cttcttttct
    20521 tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta agaagatggc
    20581 atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca ctgaaatgtc
    20641 tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt ctagattttt
    20701 tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat ccactttgat
    20761 caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat cattagggat
    20821 tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag aataaattcc
    20881 cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt ctttattgta
    20941 attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat ctgctaaagc
    21001 cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt tctccttttt
    21061 caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc ctagttttac
    21121 gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg ccaaccaaat
    21181 aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt ctcctccact
    21241 ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa aaaactaaga
    21301 ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta taccctctcg
    21361 atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta gtcagtccga
    21421 tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct tctaacaaat
    21481 acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct tcgatcaccc
    21541 caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga acaactccaa
    21601 aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag aaacgagtag
    21661 tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata ttgatcagct
    21721 tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt gtcactacta
    21781 acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa aaagacatat
    21841 atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca ttgggcaaat
    21901 atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat acgactatct
    21961 aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata atccaatgct
    22021 tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa cggcgtttct
    22081 tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt gctgtacaga
    22141 tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac cagccattta
    22201 ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc ttggtcaaga
    22261 tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct gaagatctgg
    22321 atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt gttgcagatt
    22381 tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa ctggcgattc
    22441 aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc actggttgct
    22501 tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc tactcgttgt
    22561 ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa accgacaagt
    22621 tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga atactttaaa
    22681 gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg ctgaaagatc
    22741 ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact cagattcgtc
    22801 tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac acgaactaac
    22861 gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt ttcaatcgtt
    22921 aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaaca
    22981 tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc aagaaaatga
    23041 aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc aagctgtgtt
    23101 cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc gcagccgtca
    23161 tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat agtcgttcct
    23221 tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac ttaccatttc
    23281 aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt cagtaaaagg
    23341 acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc caacatctca
    23401 aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc tacttatcga
    23461 caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca ccagccagag
    23521 cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat tttgttgttg
    23581 ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt attttttcta
    23641 tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat cttttccttc
    23701 cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa aactggaatc
    23761 aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta ctaaagaatc
    23821 ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg tcagataagg
    23881 aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt ccaagtaatc
    23941 agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg tcgtagcaat
    24001 gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat atggttttaa
    24061 gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg ctcggacttc
    24121 ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt gtttccagtt
    24181 ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt gctgaaatcc
    24241 ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat ctaaatcaaa
    24301 tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag tttagcacga
    24361 ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag ttcttggaat
    24421 cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat gactgtagca
    24481 aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc accaaaactt
    24541 gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa catggcaaat
    24601 gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag cactaaggtt
    24661 tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa aatcactcgc
    24721 tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat aacaaaatac
    24781 atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta aaccatgaat
    24841 tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt atccatccga
    24901 tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa aagttgtaaa
    24961 ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa catattgcca
    25021 aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt ttctcgtcca
    25081 gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac gtctgcagat
    25141 tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac tttcattgca
    25201 ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt acgtaaagct
    25261 gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg gtcttcagct
    25321 acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac caattcattg
    25381 atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc cccagtcaga
    25441 acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt tgtttctttt
    25501 ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc tttgactgaa
    25561 aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg taagccatct
    25621 tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt taatggtgta
    25681 acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat cgcgcctttt
    25741 gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg acgacgttca
    25801 aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc taaagtttct
    25861 gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg atagtagcta
    25921 tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc catatgatat
    25981 tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa cacatcgatt
    26041 gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt ggccatcaca
    26101 cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg tgttagacca
    26161 actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt aagcccattg
    26221 atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat ggatgtttta
    26281 tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc tgctacatga
    26341 ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc actgatgaca
    26401 ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc gtcttcacat
    26461 actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg ggaaatgaat
    26521 aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc tgaaagccga
    26581 acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc attacgtttt
    26641 acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt ggaacgtacg
    26701 gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat ggctacaccg
    26761 aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga aataattcct
    26821 aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc tacaatgaat
    26881 ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga acgttcgatt
    26941 tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc ttgtttcgtt
    27001 tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat ctcaatctct
    27061 cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat tcatcggatc
    27121 gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg cctttcctct
    27181 agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta ctaagtaatt
    27241 ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga ccacctcctt
    27301 tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa atactcagcg
    27361 aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga gttttgacac
    27421 tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc ttatctcgac
    27481 aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct atgtttgtat
    27541 aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca actaaaaaag
    27601 aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac acgttatatt
    27661 cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata gaaatatagt
    27721 aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga cggcgtgaga
    27781 cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc cattgctgaa
    27841 atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca cttgactgag
    27901 cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt ttatcttgtg
    27961 ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac aaaagctgcc
    28021 gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga aaacatcatt
    28081 cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt cccgccattc
    28141 gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac tgctggtcta
    28201 gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt gattctttcc
    28261 taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt cttcccctaa
    28321 agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga tcactgtact
    28381 tctttcttta tgtgataagc tccgtttcat atcttcattc acactacgtg cctttttcaa
    28441 ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga gatactgctc
    28501 atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat aattgttctc
    28561 aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt caaattgacc
    28621 atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac gtaaaaattc
    28681 aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa ccaaataata
    28741 cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat aaggatcaaa
    28801 gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta ttggttcatc
    28861 cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct ctaaaaatga
    28921 tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga ctagagaagt
    28981 cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga ctcgaagttt
    29041 cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt tttccacatt
    29101 caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa tgtttttttc
    29161 ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg ttggtttgat
    29221 tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat ataagttggt
    29281 tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt tttgcttttc
    29341 gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat caatcgtctc
    29401 aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat ctgaaagact
    29461 taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc ctgctgcttt
    29521 cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga cggtgtattt
    29581 gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag aagataataa
    29641 gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta gtaatgtcgg
    29701 caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat aaccaggcat
    29761 atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc atgtatctgg
    29821 atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc caaaatgatc
    29881 cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat acgtaaaaaa
    29941 ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta ctgtgctatt
    30001 gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag gcgtgtggtt
    30061 ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat attgggcaga
    30121 ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg attcatcaaa
    30181 ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat ctctattcca
    30241 aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt gtccaaggat
    30301 cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg agccttgagg
    30361 taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc cctgtcctac
    30421 ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac tatttaaaaa
    30481 cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga aactttctaa
    30541 atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat ttggctctgc
    30601 ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg catgcgcgcg
    30661 aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat cgattcctag
    30721 atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac ggttggcttc
    30781 gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag cgatagccat
    30841 cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc aaaccagtag
    30901 cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca tcaaagaaaa
    30961 ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt tgactgtaat
    31021 gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg tatttaaaat
    31081 atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca aagaaaggcc
    31141 aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact ggatcgtacc
    31201 ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc agaaaaagaa
    31261 aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt gtgtcacgta
    31321 tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga aaatttccta
    31381 aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt ccacttttca
    31441 cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa gtcttgtggt
    31501 gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca aaaaaagagg
    31561 ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct ttttcacaac
    31621 ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa tatttataaa
    31681 acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg gcaggctcaa
    31741 gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat gtcaggacgc
    31801 taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca
    31861 accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct cagagttaaa
    31921 cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg gtattagctc
    31981 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    32041 acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt ccttgtgatg
    32101 actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt tgtccactgc
    32161 cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca gttgttttct
    32221 tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact tttttgtcta
    32281 ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact ttgtcgccaa
    32341 catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt ctgagagaaa
    32401 tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct tttggcaaat
    32461 aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc caccaataag
    32521 ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt ttttctgttt
    32581 ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa ataagcagta
    32641 agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg ctttcctcca
    32701 ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa gacaaaaaag
    32761 ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa tggagaattt
    32821 cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat ctgatacgtc
    32881 tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta agtctactga
    32941 aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca tacatgtcca
    33001 catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat atttcagatg
    33061 agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt tgataaatcg
    33121 tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat ttccataatg
    33181 atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc atccgcgata
    33241 aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag gcattggacc
    33301 gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt aataattgat
    33361 acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat ggatcacagg
    33421 catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc cttttacagc
    33481 tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt taacatcttc
    33541 agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac caataacacc
    33601 accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg cttcatgata
    33661 gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc gatataaata
    33721 acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta ctttcgaacc
    33781 aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt ctcggttcaa
    33841 ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat tcacaaaggg
    33901 ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa cggcttcttc
    33961 gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg ttcgaaatga
    34021 attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat aaacttgcga
    34081 ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt accaataatc
    34141 aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg ggccacttgc
    34201 tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag cacctctttt
    34261 tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca atcacttttt
    34321 cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct cgttccccga
    34381 ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg tttttatcta
    34441 aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct tgattgaaac
    34501 attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt gccccttttt
    34561 ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct gtaaagacct
    34621 ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa cgttctcttg
    34681 ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct tgttcaccat
    34741 gatgagcgat cacttttgct acactttctc gatcattcac taatattgta tcaatacctg
    34801 ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg agcatttcaa
    34861 atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca tgcttcatca
    34921 ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt aataataagc
    34981 tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac ccaaaacggc
    35041 tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc aaccctttag
    35101 caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt ttcatgttct
    35161 tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa acaccagcca
    35221 tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta catttgattt
    35281 ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc ttgtcatcaa
    35341 tatccatata actaaagtct tcagtagagc aacttcctct cactattaat tatacttaaa
    35401 caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg caacactttt
    35461 ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaatt
//
LOCUS       DAZUKD010000018        35201 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000018 DAZUKD010000000
VERSION     DAZUKD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.018"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..185)
                     /locus_tag="ACG8N9_000716"
     CDS             complement(<1..185)
                     /locus_tag="ACG8N9_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896340.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRT"
     gene            347..1456
                     /gene="pdhA"
                     /locus_tag="ACG8N9_000717"
     CDS             347..1456
                     /gene="pdhA"
                     /locus_tag="ACG8N9_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4896341.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1459..2436
                     /locus_tag="ACG8N9_000718"
     CDS             1459..2436
                     /locus_tag="ACG8N9_000718"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4896342.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2461..4104
                     /locus_tag="ACG8N9_000719"
     CDS             2461..4104
                     /locus_tag="ACG8N9_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4896343.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4112..5518
                     /gene="lpdA"
                     /locus_tag="ACG8N9_000720"
     CDS             4112..5518
                     /gene="lpdA"
                     /locus_tag="ACG8N9_000720"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4896344.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEITILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5680..5955
                     /locus_tag="ACG8N9_000721"
     CDS             5680..5955
                     /locus_tag="ACG8N9_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4896345.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5961..6728
                     /locus_tag="ACG8N9_000722"
     CDS             5961..6728
                     /locus_tag="ACG8N9_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4896346.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6896..8725
                     /gene="typA"
                     /locus_tag="ACG8N9_000723"
     CDS             6896..8725
                     /gene="typA"
                     /locus_tag="ACG8N9_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4896347.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            8986..10758
                     /gene="recQ"
                     /locus_tag="ACG8N9_000724"
     CDS             8986..10758
                     /gene="recQ"
                     /locus_tag="ACG8N9_000724"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4896348.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10872..11174
                     /locus_tag="ACG8N9_000725"
     CDS             10872..11174
                     /locus_tag="ACG8N9_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4896349.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11177..12340
                     /locus_tag="ACG8N9_000726"
     CDS             11177..12340
                     /locus_tag="ACG8N9_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4896350.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12366..15794
                     /locus_tag="ACG8N9_000727"
     CDS             12366..15794
                     /locus_tag="ACG8N9_000727"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4896351.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISPGDLLIEVTEK"
     gene            15815..16939
                     /locus_tag="ACG8N9_000728"
     CDS             15815..16939
                     /locus_tag="ACG8N9_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4896352.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16920..17366
                     /locus_tag="ACG8N9_000729"
     CDS             16920..17366
                     /locus_tag="ACG8N9_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4896353.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17350..17688
                     /locus_tag="ACG8N9_000730"
     CDS             17350..17688
                     /locus_tag="ACG8N9_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4896354.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17807..18367
                     /gene="rsmD"
                     /locus_tag="ACG8N9_000731"
     CDS             17807..18367
                     /gene="rsmD"
                     /locus_tag="ACG8N9_000731"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4896355.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18364..18855
                     /gene="coaD"
                     /locus_tag="ACG8N9_000732"
     CDS             18364..18855
                     /gene="coaD"
                     /locus_tag="ACG8N9_000732"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4896356.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18845..19888
                     /locus_tag="ACG8N9_000733"
     CDS             18845..19888
                     /locus_tag="ACG8N9_000733"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4896357.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20047..20775
                     /locus_tag="ACG8N9_000734"
     CDS             20047..20775
                     /locus_tag="ACG8N9_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4896358.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20891..21136
                     /locus_tag="ACG8N9_000735"
     CDS             20891..21136
                     /locus_tag="ACG8N9_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4896359.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21133..21351
                     /locus_tag="ACG8N9_000736"
     CDS             21133..21351
                     /locus_tag="ACG8N9_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4896360.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21487..22026
                     /locus_tag="ACG8N9_000737"
     CDS             21487..22026
                     /locus_tag="ACG8N9_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4896361.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22019..24292
                     /locus_tag="ACG8N9_000738"
     CDS             22019..24292
                     /locus_tag="ACG8N9_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4896362.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24330..25376
                     /gene="holA"
                     /locus_tag="ACG8N9_000739"
     CDS             24330..25376
                     /gene="holA"
                     /locus_tag="ACG8N9_000739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4896363.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25465..25719)
                     /gene="rpsT"
                     /locus_tag="ACG8N9_000740"
     CDS             complement(25465..25719)
                     /gene="rpsT"
                     /locus_tag="ACG8N9_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4896364.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26109..26387)
                     /locus_tag="ACG8N9_000741"
     CDS             complement(26109..26387)
                     /locus_tag="ACG8N9_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896365.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPECLY"
     gene            complement(26649..26777)
                     /locus_tag="ACG8N9_000742"
     CDS             complement(26649..26777)
                     /locus_tag="ACG8N9_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896366.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(26857..27312)
                     /locus_tag="ACG8N9_000743"
     CDS             complement(26857..27312)
                     /locus_tag="ACG8N9_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4896367.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27450..29378)
                     /locus_tag="ACG8N9_000744"
     CDS             complement(27450..29378)
                     /locus_tag="ACG8N9_000744"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4896368.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(29575..30408)
                     /locus_tag="ACG8N9_000745"
     CDS             complement(29575..30408)
                     /locus_tag="ACG8N9_000745"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4896369.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(30600..31304)
                     /locus_tag="ACG8N9_000746"
     CDS             complement(30600..31304)
                     /locus_tag="ACG8N9_000746"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4896370.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(31327..31953)
                     /locus_tag="ACG8N9_000747"
     CDS             complement(31327..31953)
                     /locus_tag="ACG8N9_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4896371.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            32279..34654
                     /locus_tag="ACG8N9_000748"
     CDS             32279..34654
                     /locus_tag="ACG8N9_000748"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4896372.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(34678..35169)
                     /locus_tag="ACG8N9_000749"
     CDS             complement(34678..35169)
                     /locus_tag="ACG8N9_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4896373.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
BASE COUNT        11672 a         6213 c         7345 g         9971 t
ORIGIN      
        1 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
       61 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
      121 gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
      181 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
      241 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
      301 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
      361 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
      421 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
      481 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
      541 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
      601 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
      661 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
      721 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
      781 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
      841 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
      901 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
      961 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
     1021 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
     1081 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
     1141 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
     1201 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
     1261 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
     1321 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
     1381 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
     1441 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
     1501 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
     1561 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
     1621 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
     1681 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
     1741 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
     1801 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
     1861 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
     1921 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
     1981 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
     2041 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
     2101 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
     2161 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
     2221 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
     2281 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
     2341 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
     2401 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
     2461 atggcttatc aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
     2521 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
     2581 aatgacaaat cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt
     2641 gttccagaag gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga
     2701 cacgaagaca atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg
     2761 gctgctgaac ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa
     2821 tttaagttac cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc
     2881 aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca
     2941 gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc
     3001 actgtagcaa atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca
     3061 ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca
     3121 gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt
     3181 caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc
     3241 agagtaacaa aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg
     3301 aaatcagaag cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa
     3361 ccagctgcac cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca
     3421 atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca
     3481 cacgtgacat tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt
     3541 aaagaagttg ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct
     3601 ttaacagcga ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa
     3661 gaaatcgttt ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta
     3721 tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc
     3781 aatgagaaag caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga
     3841 acaattacga tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac
     3901 tatccagaag tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct
     3961 gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc
     4021 gtagatggag caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca
     4081 gagctattaa tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt
     4141 agatacagtt gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga
     4201 aatgggacaa aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt
     4261 cggctgtatt ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga
     4321 ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga
     4381 atggaaagaa aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa
     4441 acataaggtt gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt
     4501 cattcatcca gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc
     4561 ccgtccaatc gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg
     4621 attagcattg aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc
     4681 cgaattgggc ggagcgtacg caaacttagg agcagaaata acgattcttg aaggctcacc
     4741 acaaatttta ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa
     4801 aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag
     4861 cgtgacagtg aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat
     4921 ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga
     4981 agtaggagaa cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat
     5041 ctatgccatc ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc
     5101 aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc
     5161 ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa
     5221 agatgctggt atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt
     5281 atcgcttggt aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct
     5341 tattggcgcg caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc
     5401 aatcgaatca ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct
     5461 tggcgaaatc gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt
     5521 tgaataatac aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc
     5581 aataatatta ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta
     5641 tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca
     5701 cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg
     5761 gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt
     5821 aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct
     5881 tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg
     5941 agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag
     6001 aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc
     6061 gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc
     6121 attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg
     6181 atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg
     6241 aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt
     6301 tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata
     6361 aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg
     6421 tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca
     6481 tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc
     6541 acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg
     6601 aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg
     6661 aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag
     6721 aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat
     6781 tttctcgaaa aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc
     6841 agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa
     6901 ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac
     6961 attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga
     7021 acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa
     7081 cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga
     7141 cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga
     7201 tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca
     7261 tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt
     7321 cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt
     7381 tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga
     7441 tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt
     7501 cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt
     7561 aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc
     7621 attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt
     7681 tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg
     7741 tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc
     7801 tatccttcac attgacgaac caacattgca aatgactttc ttagttaata attcaccatt
     7861 tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga
     7921 acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt
     7981 ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata
     8041 cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga
     8101 accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc
     8161 cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg
     8221 tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat
     8281 gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg
     8341 tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac
     8401 ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt
     8461 atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac
     8521 gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa
     8581 aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg
     8641 cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga
     8701 aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta
     8761 taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc
     8821 aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat
     8881 ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc
     8941 ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta
     9001 aaacaatact ttggctatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc
     9061 attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac
     9121 cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg
     9181 atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc
     9241 tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt
     9301 ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga
     9361 tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat
     9421 ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta
     9481 attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta
     9541 aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa
     9601 gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat
     9661 caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt
     9721 aaaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca
     9781 gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt
     9841 ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct
     9901 ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc
     9961 atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa
    10021 agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg
    10081 tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg
    10141 tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca
    10201 cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg
    10261 aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct
    10321 acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg
    10381 atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa
    10441 agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc
    10501 cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg
    10561 gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa
    10621 gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc
    10681 caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct
    10741 aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa
    10801 tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga
    10861 ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag
    10921 cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca
    10981 aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg
    11041 ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac
    11101 gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg
    11161 gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat
    11221 cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc
    11281 gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt
    11341 ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa
    11401 aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt
    11461 gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat
    11521 ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa
    11581 aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt
    11641 tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt
    11701 tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt
    11761 aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg
    11821 aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc
    11881 tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa
    11941 cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga
    12001 agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat
    12061 tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc
    12121 aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt
    12181 atttgtcaat cttggaggga ttactggcgt tattcctttg acagggatca cgttcccatt
    12241 tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa
    12301 catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg
    12361 gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg
    12421 cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg
    12481 tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg
    12541 cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc
    12601 atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag
    12661 gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag
    12721 caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg
    12781 cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg
    12841 cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag
    12901 agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg
    12961 ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg
    13021 gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag
    13081 tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag
    13141 ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg
    13201 aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca
    13261 cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg
    13321 atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca
    13381 ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa
    13441 tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg
    13501 cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg
    13561 ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg
    13621 gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag
    13681 gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac
    13741 gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag
    13801 gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca
    13861 caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg
    13921 tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc
    13981 atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga
    14041 ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg
    14101 tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa
    14161 tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt
    14221 atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat
    14281 ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac
    14341 gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa
    14401 gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg
    14461 gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc
    14521 gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata
    14581 ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg
    14641 acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt
    14701 atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa
    14761 accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc
    14821 ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac
    14881 aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg
    14941 tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata
    15001 tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa
    15061 cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg
    15121 gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac
    15181 gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa
    15241 aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct
    15301 ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa
    15361 catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa
    15421 cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt
    15481 tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta
    15541 cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt
    15601 ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt
    15661 tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag
    15721 tcgcacatgt atatgtgtct tcaggagaca ctatttcgcc tggtgacctt ttgattgaag
    15781 tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct
    15841 gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact
    15901 tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca
    15961 ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa
    16021 ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta
    16081 tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac
    16141 tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat
    16201 gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac
    16261 agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga
    16321 taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt
    16381 gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct
    16441 tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg
    16501 aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca tatacaatca
    16561 aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga
    16621 ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac
    16681 gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt
    16741 agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata
    16801 ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg
    16861 cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag
    16921 tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg
    16981 ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg
    17041 aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg
    17101 aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc
    17161 gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa
    17221 cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg
    17281 aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag
    17341 gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc
    17401 gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat
    17461 gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca
    17521 gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat
    17581 atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc
    17641 gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg
    17701 agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta
    17761 tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc
    17821 aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac
    17881 agataaagta aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt
    17941 attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag
    18001 tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga
    18061 aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga
    18121 acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa
    18181 acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc
    18241 agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca
    18301 tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa
    18361 tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat
    18421 acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact
    18481 tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct
    18541 catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa
    18601 gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa
    18661 cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta
    18721 gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc
    18781 ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa
    18841 tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat
    18901 cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga
    18961 tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct
    19021 tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc
    19081 ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa
    19141 tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa
    19201 acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa
    19261 aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa
    19321 aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac
    19381 aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga
    19441 gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga
    19501 atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat
    19561 gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat
    19621 tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa
    19681 aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat
    19741 ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc
    19801 tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc
    19861 gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga
    19921 gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga
    19981 aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa
    20041 ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg
    20101 atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg
    20161 atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga
    20221 gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat
    20281 ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat
    20341 atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat
    20401 gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt
    20461 tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca
    20521 gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct
    20581 cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga
    20641 tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg
    20701 gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc
    20761 gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg
    20821 gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat
    20881 aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct
    20941 ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca
    21001 agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat
    21061 tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa
    21121 aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt
    21181 atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa
    21241 gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt
    21301 atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg
    21361 gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt
    21421 aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata
    21481 taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat
    21541 aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta
    21601 tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg
    21661 atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg
    21721 tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta
    21781 cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca
    21841 tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat
    21901 gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac
    21961 caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat
    22021 gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat
    22081 gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat
    22141 ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag
    22201 cataacggta tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac
    22261 atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat
    22321 ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga
    22381 agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg
    22441 cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt
    22501 caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca
    22561 aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc
    22621 tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc
    22681 aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct
    22741 atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc gacgtatcgg
    22801 atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat
    22861 tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt
    22921 caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct
    22981 tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag
    23041 ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt
    23101 cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt
    23161 aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt
    23221 gttatttctc acactcttca atcgctggct gccggaaact ttacttttgc tgatagaaga
    23281 gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg
    23341 acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag
    23401 gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt
    23461 gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga
    23521 cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca
    23581 gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa
    23641 tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca
    23701 cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa
    23761 aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc
    23821 tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta
    23881 tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg
    23941 tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat
    24001 ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca
    24061 tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg
    24121 gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac
    24181 actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg
    24241 ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc
    24301 atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt
    24361 tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt
    24421 ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg
    24481 acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagctgaag
    24541 cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt
    24601 taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg acggcttatt
    24661 tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg
    24721 aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt
    24781 tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa
    24841 tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga
    24901 tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag
    24961 aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg acccaatata
    25021 ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg
    25081 aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa
    25141 aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc
    25201 catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac
    25261 agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag
    25321 aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag
    25381 aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa
    25441 aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc
    25501 gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac
    25561 agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt
    25621 tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct
    25681 tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt
    25741 aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg
    25801 ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt
    25861 cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat
    25921 agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt
    25981 tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat
    26041 acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta
    26101 tcctttgatc agtaaaggca ttctggtgta ccgattattt taatgtattc atcgatattt
    26161 tttatcaaca ttttttttat gaccaactgg ttccgacggt tccatttgcc acttacttcc
    26221 agcacatatt tatttagggg caaacgtaag atatattttg ccaaatagcg atctttaagc
    26281 aggccattga tcacacggtc ctcaagctgc aagttgatcc cagtcagcat cattggctgt
    26341 tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag tttccacaag cgtcttcctt
    26401 tcttttcaaa gtaacctgaa aacaggcact aattcttcca ttttgatatt cgttgaagtg
    26461 gatgcgatca ttctttcttt tgtccactgt aatctagtat aacaaaaaag agcagaagag
    26521 ccaaaaagaa aaaagagcat cgaattctat aggaagtctc attggcacgg cctaaattat
    26581 tacaaaacat tgattaaaaa aagcccctgc atcgcaggag catcccctat atcgttttaa
    26641 taggtcaatc aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa taatagataa
    26701 gctaagccca gaatcaaaag taccgctaga agaaaccagc cgattgttcc tatgaaatac
    26761 tttaattttt cgaacattgt atcacctcgt tgtcagtata tcacggatca gtaaattggg
    26821 ggtgttaaga atctcctaaa atagacaaaa tttgttctat tttcctaaag caaatacttg
    26881 taagatacag taagtgatac cagaagcaat gaccggtccg gcggcgattc ctcttaagaa
    26941 gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat cagaagcaat
    27001 caatcctaca cccttggaag ataagacagc cactagccca ccgcaaaaaa cagctaccca
    27061 gccaataggg gatttaaagg cttctaatag atcacgtaga ccgatttctc ctgttgcgat
    27121 agggattaga atagccgctg tgatgatcgt caccccaata ttgattcctt tgccttgaag
    27181 ccaagggaaa aaggaacggg aggctggtat gagcttcata atcaaaacaa aagctgccgc
    27241 tatcatcaga ctttgatttt tagctaaaat tgctacaagt aaaataattc ctaaaaaaag
    27301 ccaactttcc atgaaatata ttcctcactt tcttcgaaac aatcataaca tgtttttaga
    27361 aagttacaaa agtaggaatt catccttcaa gacctattca taagctgttt ttttcataaa
    27421 ataaacaaaa tcataaaaaa acactttgct tatttgacag aagacagccg cgctttgtat
    27481 agtggacaag acttacattt ttttgaagaa tcagaaagat caggtaaagg tcttagtaaa
    27541 cgcaagccat tcagaataac caatattgtg cttccttcgt gtcctacaac tccaagaggt
    27601 agatttaaaa attgcaggaa gttagaagca atcaacaaga gaataacaag aatcgaaaaa
    27661 ataatatttt gtttgatgat ccgccgcatt tttaaagata gccgatgact gtagacaaat
    27721 ttatttaagt cgcttttcat taagacgatg tctgcgatat ccatagcggc atctgtccct
    27781 tgccccatag cgacacctaa agaagcggct gctaaagcag gcgcatcatt gactccatca
    27841 ccaaccatcg catttacttg gtaaagttca ttctcttttt tgactagttg tgttttttct
    27901 tccggagtac agcttgcata gtaatgttca atccctactt gttcagcaat ggcctttgct
    27961 gtgccagaat ggtcgccggt tatcatactt gtatggatgt tttgggattt aaagtaggaa
    28021 attacttgtt gggtatttgc ttttggaata tctagcagac ctaaaacagc aattaactgc
    28081 tgatctttag aaagataaat cacggttttg ccttgatttt ccaaacgttc gatcatttcc
    28141 gcaatctctt tgctgattat catcgtctca ggatcatatg cgtgcttgcc aactttccat
    28201 tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa tttcttctat tggtaagtat
    28261 tggatttctt caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc aagtggatgc
    28321 gtcgtttttt gttccatagc aacaagaagt tgttgtgcca aatccttttc atcaaagaaa
    28381 aagctgtcag ttacaactgg ttttccttta gtgagcgttc ctgttttatc aaaggcaatc
    28441 gcttttaatt cagaaagctt ttctaaatgg ataccgcctt tgattaaaat cccatttttt
    28501 gcagaatttg aaagagcagc tagagaagct ggtgtagcag atgcgacaag cgcacaagga
    28561 gaagccacaa ctaataatac cattcctcga taaaagcttt catcccaact ccaacctagg
    28621 aaaaagtggg ggagaaggat cattagagga acagcaagca aaatcaattt tacataatta
    28681 ttttcgattc gttcgataaa tgaagctgtt tgagttggcg tattctgcgc ttcttctact
    28741 aattggataa tcttagcaaa gacagtttcg gaactcgttt ttgttactcg aatcgtgatg
    28801 ggatttcctt gattcagcgt tcctgcatac atctcatcgc ctaaatattt ttctacaggc
    28861 acagattcac cgcttaatgc agactcatcg atcgttgcct gtgtgctttc taaaaagcca
    28921 tctatcggaa cagctgctcc tttagcgaca aataccaagt tatccacctt tagttgagaa
    28981 acatctactt cttgcatttg tccattttct tttaataatt gggctttttg tggttgaaga
    29041 ttcatcaaag cagtaatttc tttttgactt ttattagttg tgtattcctc taatgcaccg
    29101 cttaaacaaa agatgaatgt caacattgct ccttcaaagt aattaccgat caaacaggca
    29161 ccaatagcag ctaaagccat caataaatcc acattcagtg tccgatcatt ccacaattct
    29221 ttcaagcctt ccgatgtttg tttatatcca ccgatgataa tggcactgat ataaaaaagt
    29281 ggggtgatgg taagagagaa tcggtcgaag ataaaaccga agatcataaa taaaaggcag
    29341 ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg ttattagacc tccttgtcaa
    29401 aacaaggata gcataataaa aatgtaattg ataataataa cagctaaatg agaacgataa
    29461 tggttatcat ttggtgatta agcttgatgt tgtttttatt atttataatg attattatta
    29521 aagaaaataa aaaaccagaa catttctcaa ataagagaag tgttctggtt ttttctacat
    29581 tgcaataaac caataaccaa tcaaaatgat accaaggata atacgatacc agccaaaagc
    29641 ggtaaagtcg tttttcttca aatagcttag taagaatttg atagctaaga cggaaaccac
    29701 aaaagatacg aatgttccta caagaagaac aacagtttct tccattttga atgaattccc
    29761 ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca aacatgacag gaatccctaa
    29821 gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca atcaagatag atcctaggat
    29881 cgttgcgcca gaacgggatg ttccgggaac aagtgccaag acttggaata acccaacaag
    29941 aagagctgct tggtacgtaa agtcattcag agaagtccaa cgaggtgttt tgtttttgtt
    30001 gcgtttttcg acaataataa aggcaatacc gtaaacgatc aaaacaatgg caactggtaa
    30061 gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc aaaccaatga tgatggctgg
    30121 tatgcaagca acgatcactt taaaccaaag tgtccacgta tctttttttt ctaccgttga
    30181 tttacttgga gaaaatgggt ttaacttgtg aaaataaaga acgacgacag ccaagatcgc
    30241 ccctaattgg ataacgacat tgaacatcga cataaattgt gaactggcat ccagtttgat
    30301 gaattcatct gctaggatca aatgaccagt actactgatg ggcagccatt ccgttattcc
    30361 ttcaataata ccgagaatca ctgctttgat aatatttgca aaaaacataa attccttcct
    30421 tcccaattaa gatacatctt atagtatacc gttatttttt gagaaaccca atctgttttt
    30481 taatattaag aaaaacttga gaaataaagc agataagtac acgaagagaa aaaagaagac
    30541 aaagccctcg ttcagacttt gccttttatg attctctggt aaaaattaaa atatccccat
    30601 tatagaaacg agtcgcgact atgtgttttc ccgttatcga tataagacgt atcattgatg
    30661 ccgtggatag aaaatcgacc gtttgcataa gtgacctttg ttacgctgcc atttaaaagc
    30721 ccagcacgaa caggctgttt aggatcgatc agattaagca aaaaggcgat cgtcaaacca
    30781 tgagatacga ctaaaacatt tccaccacca tttttttcgc agtgatgggc gatatcttca
    30841 aatcctgtcc atacacggtc tcgcaatgtt tcataagttt ccgcccaccc agcggtatct
    30901 aagttgtata cagcattagc aatttgttca aaagaaactt ctgtggtaaa catttcatca
    30961 tatgttttaa aattcaatac acgagggatc acaccccaca tttccatgtc atatccacct
    31021 tcaagagacc cgaaacacca ttctctaatc cttttttcaa taaaataagg aatttctttt
    31081 cccttagaat gttctcctag aataatacgg gcagtttcga ttgcacgacc actgtcacta
    31141 gaataagcaa atgaaaagtc gacatttttt aacccgcgac ctaaataatg aatgccttct
    31201 tgtccttgct ttgtgagggg tgtatcacac cagccttgta ctcgttcaat tgtattgaac
    31261 atcgtttttc cgtgtcgtac gatataaagt gtagtttctg tcattttatt ctcctcctaa
    31321 gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc tgttcaatcg tcgttgcatc
    31381 gccatgtcct ggatagactg caaattcatc aggtaataca aacagctggg tccgaatact
    31441 atgaagtaat tgttctaaat tccctgtata cagatcggtt ctaccgatac ttcctctaaa
    31501 cagggcgtcg ccagaaacga caaaatcatc aaaaataaag ctgacgcttc ctatagaatg
    31561 ccctggggta gggacgacgc gaaaagacat gtttcctaaa gtataagttt tcatttcaaa
    31621 ttcatattct gcaggcgaga caataacatc agcaatatca tcatgtctac cgagtccaga
    31681 aagattcaaa atcggatcac ctagccattt ttgctctagt gggctgacat aaactgggat
    31741 ttgataatac gcgcgtaaag gttccactgc accgatatga tcgtagtgag tatgggtaag
    31801 caaaatcgct accggctgtt ggtttgtttt tttgatttct tgtttgattc gttccgcatc
    31861 ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag accaaataac agttttcttc
    31921 aatcattcct gtttttaatc tttcgatatg catgtgcatt cctccttgat agccaaaact
    31981 tctttcatcc attagtataa cgcattcatt gctaggaaac aaaaacaggt acttatttct
    32041 ctttacttat ctgaattatc agactatatg aaaagtattt ggatattcta aagagaagga
    32101 aagtaagttt tcatagcatt ttaatatgtc cttgttacat tagattcatc caaaaatcca
    32161 aattctatat cctatatccc tgcctaatca gcagggtgtt tttttatgtt tggaaagaga
    32221 gctctttttg ctaaactagg ttttttcagg taatcttaga gagaaagagg tggtggaaat
    32281 gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa tttattttgc gacgtggaag
    32341 tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa ggagcgagaa tccaccgcaa
    32401 gctccaaaaa gatgcgggag aaaactatga aaaagaagtc tttttaaaaa caacagttca
    32461 attagatgaa tattacctga tcgttgaagg ccgtgcagac ggtattttca aaagagacgg
    32521 tatttattat atagatgaaa tcaaaacctc cgaaccacga tttgaagatt tagaaccaga
    32581 acaagtggaa cttttctttc atcaagcaag ggtgtatgcc tatatctata gtcaagaaca
    32641 tgatttacaa gaaataaatc tgcaattgac ttacttccag acggcagaag aagtgatcac
    32701 gagaaaggtt gaacatcaga caagagagca gttagaaact ttttttaaaa agctgactga
    32761 agattataaa gaatggcttg tttttcaaga aaactggcgc actgtacgca atgcctcttt
    32821 gatggctttg aaattccccc atgatgagta taggaaaggt cagcgtgaac tagcggtagc
    32881 tgcttataaa acgatccgta cgaaacagaa attgtttgta gaagcaccta ccggaacagg
    32941 aaaaacaatc tctacgttat tccccgcttt gaaagctgta ggggaagaag agggagaaaa
    33001 gatcttttat ctaacggcta aaacaataac tagacaagta gctgaagatg ctatgacagc
    33061 attaaaagat acaggagctg aagtaaaaag tgtcactttg acggcgaaag acaagatctg
    33121 ctttttagat gagacgacct gtaatccaga tcagtgccca tatgccaatg gttattacaa
    33181 tcggattaat gaaggtttat gggatctttt gaatcatgag aatcagatca ctcgtgaagt
    33241 catcgaaact tatgcaagaa agcatacact ttgtcctttc gaactatcat tagatgtcag
    33301 tatctggtgt gacgtaatca ttggggatta caattattta tttgatccaa ctgtttattt
    33361 aagacgtttt tttgaagatg aaaaaaacga ggactatctg tttttgattg acgaagcaca
    33421 taatttggtg aatcgttccc gagagatgta ttcggcagaa ctatcttatg aaaaaacgaa
    33481 acgaagtaag gaagctatac caaaggaatt caaaaaactt catcgccgtt tcaacaaact
    33541 attaaaagaa tttgattcga tccgagaaat agcaaaagaa gatcattggg attatcatca
    33601 tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat caattaagcg aaaaaattaa
    33661 agaatggctg gcagaatttc ctgagcaccc atcacaagaa caattgcttc cttattattt
    33721 tgatttgctt cattttttga aggtcagtga attttatgat gatcactacg aaacaacagt
    33781 cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt attgatccaa gcttgtttct
    33841 tgagcagtcg ttggataaag gaaaaagcag tattcttttt tctgctagtt tttcaccact
    33901 ctcttattat caggaaacac tggggggaca aaaaagtctg gcttacaaac tgccgagtcc
    33961 tttcccagaa ggaaaccagc aagttttcat cgcaaattac ctagagacga cttatcgaaa
    34021 aagggaacac agtctggcaa aattagtaga gacgattcat gcttttgctg atggaaaggt
    34081 cgggaattat ttcgttttct ttccttctta tcaatattta gatcttgcgg tggaagcatt
    34141 cagacagcaa tatcccgaca gcaatgtgct aatccaagaa acggacatga atgaggaaga
    34201 acgcgaacga tttttaacaa aatttcaaac aaatcccgct gaaacattga ttggattttg
    34261 cgtacttggc gggatttttt cagaaggaat cgatttgaga ggggaccgat tgatcggcag
    34321 tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag caggaactga tcaaatcata
    34381 ttatgatgag aaggaacacc ttggatttgc ttacgcttat cagcttcctg gaatgaacaa
    34441 agtcttgcaa gctggtggtc gagtgattcg tggcatggcg gatcaaggaa tcgttgtatt
    34501 ggcagatcaa cgattttcta gtttttctta tcgacgttta ttcccacaac attggcaaac
    34561 agcaagagaa gtacgaagtg ttggggaatt ggaagaagga atacagcgtt tttggttatc
    34621 aaaagaagca gtaaatcaaa aaaacgagga atgacagacg tcagcctcgt tttttgatta
    34681 acgatagtta gtgaattgca actcgattgg taagtcaagt ttacgtaata aagcaatcac
    34741 agcttgtaga tcatcacggt tcttacctgt cactctaatc tgatcttctt ggatttgtgc
    34801 ttttactttc agtttttcat ttttgatagc tgtagtgatt ttcttcgcgt tttcacgatc
    34861 gattccgttg accaaatcgg cagtttgtcg agctttgcca cctagagcgt gttctgattc
    34921 ggaaaaatgg atatttttga ttggcacatt tcttttgatc agcttaccaa ataatacatc
    34981 tttgatctgt tcgattttga aatcatcatc gctcaataca actagattgc tgttctctaa
    35041 tttgatttct actgtagaat ccttgaagtc aaagcgattg gacaattctt ttgttgtttg
    35101 ctgaatcgca ttttttactt cttctgtatt gacttctgat acaatgtcga agctagcatc
    35161 ttttgccata ggaaccctcc tgatatacat ataaatattt a
//
LOCUS       DAZUKD010000019        34564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000019 DAZUKD010000000
VERSION     DAZUKD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.019"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1183)
                     /locus_tag="ACG8N9_000750"
     CDS             complement(44..1183)
                     /locus_tag="ACG8N9_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4896374.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQHFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(1297..1881)
                     /locus_tag="ACG8N9_000751"
     CDS             complement(1297..1881)
                     /locus_tag="ACG8N9_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4896375.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(2008..2439)
                     /locus_tag="ACG8N9_000752"
     CDS             complement(2008..2439)
                     /locus_tag="ACG8N9_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4896376.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(2457..2777)
                     /locus_tag="ACG8N9_000753"
     CDS             complement(2457..2777)
                     /locus_tag="ACG8N9_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4896377.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3071..3211
                     /locus_tag="ACG8N9_000754"
     CDS             3071..3211
                     /locus_tag="ACG8N9_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896378.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            complement(3208..3387)
                     /locus_tag="ACG8N9_000755"
     CDS             complement(3208..3387)
                     /locus_tag="ACG8N9_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896379.1"
                     /translation="MNKKTDEIAADLVVAWLNHESQVSTKGESISPKEIAQAYLDIHY
                     AVIYGQLPEDRKNDD"
     gene            3468..4214
                     /locus_tag="ACG8N9_000756"
     CDS             3468..4214
                     /locus_tag="ACG8N9_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4896380.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            4229..4432
                     /locus_tag="ACG8N9_000757"
     CDS             4229..4432
                     /locus_tag="ACG8N9_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896381.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            4448..4786
                     /locus_tag="ACG8N9_000758"
     CDS             4448..4786
                     /locus_tag="ACG8N9_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS4896382.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(5002..5481)
                     /locus_tag="ACG8N9_000759"
     CDS             complement(5002..5481)
                     /locus_tag="ACG8N9_000759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896383.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            5526..6233
                     /locus_tag="ACG8N9_000760"
     CDS             5526..6233
                     /locus_tag="ACG8N9_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4896384.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            6226..6354
                     /locus_tag="ACG8N9_000761"
     CDS             6226..6354
                     /locus_tag="ACG8N9_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896385.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            6412..6753
                     /locus_tag="ACG8N9_000762"
     CDS             6412..6753
                     /locus_tag="ACG8N9_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896386.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            6746..7492
                     /locus_tag="ACG8N9_000763"
     CDS             6746..7492
                     /locus_tag="ACG8N9_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4896387.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            7498..8184
                     /locus_tag="ACG8N9_000764"
     CDS             7498..8184
                     /locus_tag="ACG8N9_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4896388.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            8187..8936
                     /locus_tag="ACG8N9_000765"
     CDS             8187..8936
                     /locus_tag="ACG8N9_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896389.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            8948..9217
                     /locus_tag="ACG8N9_000766"
     CDS             8948..9217
                     /locus_tag="ACG8N9_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896390.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            9222..9386
                     /locus_tag="ACG8N9_000767"
     CDS             9222..9386
                     /locus_tag="ACG8N9_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896391.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            9379..9681
                     /locus_tag="ACG8N9_000768"
     CDS             9379..9681
                     /locus_tag="ACG8N9_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896392.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            9678..9839
                     /locus_tag="ACG8N9_000769"
     CDS             9678..9839
                     /locus_tag="ACG8N9_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4896393.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            9836..10141
                     /locus_tag="ACG8N9_000770"
     CDS             9836..10141
                     /locus_tag="ACG8N9_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896394.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            10141..10497
                     /locus_tag="ACG8N9_000771"
     CDS             10141..10497
                     /locus_tag="ACG8N9_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4896395.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            10457..10702
                     /locus_tag="ACG8N9_000772"
     CDS             10457..10702
                     /locus_tag="ACG8N9_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896396.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            10699..11118
                     /locus_tag="ACG8N9_000773"
     CDS             10699..11118
                     /locus_tag="ACG8N9_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4896397.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            11115..11672
                     /locus_tag="ACG8N9_000774"
     CDS             11115..11672
                     /locus_tag="ACG8N9_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4896398.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            11669..11965
                     /locus_tag="ACG8N9_000775"
     CDS             11669..11965
                     /locus_tag="ACG8N9_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896399.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            12042..12455
                     /locus_tag="ACG8N9_000776"
     CDS             12042..12455
                     /locus_tag="ACG8N9_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4896400.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            12764..12916
                     /locus_tag="ACG8N9_000777"
     CDS             12764..12916
                     /locus_tag="ACG8N9_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896401.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(12913..13188)
                     /locus_tag="ACG8N9_000778"
     CDS             complement(12913..13188)
                     /locus_tag="ACG8N9_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896402.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            14046..14213
                     /locus_tag="ACG8N9_000779"
     CDS             14046..14213
                     /locus_tag="ACG8N9_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4896403.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            14239..14583
                     /locus_tag="ACG8N9_000780"
     CDS             14239..14583
                     /locus_tag="ACG8N9_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4896404.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            14588..14869
                     /locus_tag="ACG8N9_000781"
     CDS             14588..14869
                     /locus_tag="ACG8N9_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896405.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            14972..15286
                     /locus_tag="ACG8N9_000782"
     CDS             14972..15286
                     /locus_tag="ACG8N9_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4896406.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            15264..16958
                     /locus_tag="ACG8N9_000783"
     CDS             15264..16958
                     /locus_tag="ACG8N9_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4896407.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            16978..18156
                     /locus_tag="ACG8N9_000784"
     CDS             16978..18156
                     /locus_tag="ACG8N9_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4896408.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            18119..18805
                     /locus_tag="ACG8N9_000785"
     CDS             18119..18805
                     /locus_tag="ACG8N9_000785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4896409.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            18805..19965
                     /locus_tag="ACG8N9_000786"
     CDS             18805..19965
                     /locus_tag="ACG8N9_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4896410.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            19975..20850
                     /locus_tag="ACG8N9_000787"
     CDS             19975..20850
                     /locus_tag="ACG8N9_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896411.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            20847..21158
                     /locus_tag="ACG8N9_000788"
     CDS             20847..21158
                     /locus_tag="ACG8N9_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896412.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            21148..21501
                     /locus_tag="ACG8N9_000789"
     CDS             21148..21501
                     /locus_tag="ACG8N9_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4896413.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            21491..21892
                     /locus_tag="ACG8N9_000790"
     CDS             21491..21892
                     /locus_tag="ACG8N9_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896414.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            21885..22289
                     /locus_tag="ACG8N9_000791"
     CDS             21885..22289
                     /locus_tag="ACG8N9_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896415.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            22301..22909
                     /locus_tag="ACG8N9_000792"
     CDS             22301..22909
                     /locus_tag="ACG8N9_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4896416.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            22929..23291
                     /locus_tag="ACG8N9_000793"
     CDS             22929..23291
                     /locus_tag="ACG8N9_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896417.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            23294..23476
                     /locus_tag="ACG8N9_000794"
     CDS             23294..23476
                     /locus_tag="ACG8N9_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896418.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            23494..26826
                     /locus_tag="ACG8N9_000795"
     CDS             23494..26826
                     /locus_tag="ACG8N9_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4896419.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTAISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKVQNDNASAWR"
     gene            26877..27614
                     /locus_tag="ACG8N9_000796"
     CDS             26877..27614
                     /locus_tag="ACG8N9_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4896420.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            27624..29915
                     /locus_tag="ACG8N9_000797"
     CDS             27624..29915
                     /locus_tag="ACG8N9_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4896421.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREVA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            29939..32137
                     /locus_tag="ACG8N9_000798"
     CDS             29939..32137
                     /locus_tag="ACG8N9_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4896422.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPYQPNLLVEPYNMCREYPNENIADPKVKF
                     PIESGDHQIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLT
                     DVWSLTFTPTNVVAMPKLFRVYQYPRSTVGACQIDWLKIEKGNTRTPNISEYKYRGTG
                     MRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEV
                     DTTAKDAQTKANELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVG
                     ETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVAYGVD
                     ALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD"
     gene            32215..32457
                     /locus_tag="ACG8N9_000799"
     CDS             32215..32457
                     /locus_tag="ACG8N9_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFS4896423.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            32473..32670
                     /locus_tag="ACG8N9_000800"
     CDS             32473..32670
                     /locus_tag="ACG8N9_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4896424.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            32681..33700
                     /locus_tag="ACG8N9_000801"
     CDS             32681..33700
                     /locus_tag="ACG8N9_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4896425.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            33890..33963
                     /locus_tag="ACG8N9_000802"
     tRNA            33890..33963
                     /locus_tag="ACG8N9_000802"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33924..33926,aa:Ile,seq:cat)
BASE COUNT        12161 a         5339 c         7056 g        10008 t
ORIGIN      
        1 gatacagggg tttgttttgt gtttgtgcca aaaatgtgcc aaactagtta tcatttttat
       61 ataaactatc gaacacagca ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
      121 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
      181 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
      241 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
      301 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttaa
      361 cttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
      421 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
      481 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
      541 cccatgttaa tcctagaagt tcagcaaatc gcattccagt agcaccagca actaacacga
      601 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
      661 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
      721 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgatg
      781 ctctaatatg gttattaaac ttaataacgg tggattttac atggtctttc gcatacttat
      841 ttaaaaagtg ttgatatcca actctatcta aatcagaaat caataccgct ggcatatatt
      901 tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt
      961 ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc
     1021 ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt gcttcgcctt
     1081 ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa
     1141 tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa tctactctct
     1201 ttctctgata caataggcac tataaagaag cctactgtat aggtttgttt ttcttagaac
     1261 acgcttgctt tggacggtgg gcgtgttttt tatttattat acagttgctt taaaaatagc
     1321 tgctttttca aatgatacta aaggagagaa gtggatttct atttctccct tttggtttaa
     1381 cgtgaaatga gcagttacat ccatttgttt tcctggagca acagatccca ttgtattttc
     1441 gttggcatat gtctctgatt ttttgtcatc tggtccatat acttccacat ctgtacctac
     1501 agggatatct gaatcgccat cattttttac ggtataagta atttttacta cttgtgcagg
     1561 ttggttttct tcaaattgat ttctttcatc agttagttct acactattta gtgtatattc
     1621 agcatcacca acttttacag tgtcaccaat cttatagaag gtatcgcttt tttcttcttt
     1681 agaagatgaa gcagaggttg aagatttagt tactttttcg ccaccattat cacttgcttt
     1741 attactatct gatccaccat ttaaagcaga accaataatt ataattaaaa ttactgctaa
     1801 tacccaaaac catacttttt tgtaaaaagg tttactaact ttatacattt ttccgtcttg
     1861 acccataact ttttttgcca tttaaatatt cctcacttct tgttataata tatttgcgat
     1921 ctcagaaatg aggtatgagt ccgtgtgcca gcacgggctt ttttaatgtt ttgctgaaat
     1981 cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag aatatttttt
     2041 cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct gaatagaata
     2101 atactccata aatttttcga gattaaactg agattcatca gtcagttcgt tctcaatata
     2161 gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt ttgagtgaaa
     2221 agttgtagaa gtatcgtaca aaactgaata ttcaaaatga gaagcggtga aatgcgcaag
     2281 ttcatgagat aaatgaaagg cttctgcagt ttcgccatat agattttcat tcaaaaaaat
     2341 gattctaggc ttcggataat agaagccagg ctctttcatt tccatgtaaa ctactttcaa
     2401 attgtattcg ctcaacattt ctttcaattt caaatacata caacccatca ctccaactat
     2461 tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc cttagatatt
     2521 tcttttccat caaaagaaaa aacagtatcg ttttctgata aatccacatg tttaggggtt
     2581 tctttttctt cgcgccccag aagatagtct acagagacat cgaaatagtc agcaatttct
     2641 tgcaaacgtt ctgtagaggg gtttgagttc ttaagtctat ataagacgtt tttaccatag
     2701 cctaggtctt cttcaacttt atttaatgat tttccctgtt ttttcgctaa ctcttttatt
     2761 ctctcaaacg ttatcatatc aatattcctt tcgttcaaga aaaaatattt aactttattt
     2821 gttaaaattc gttgactatt ttaacaaaag gtgttaatat cattcttgta aacaagttta
     2881 acaactaaaa agacaacaaa aaacactatt gattaataaa tgccaaccgc caagaaagct
     2941 tttaaaatca atgtttatat gtcttattta actatgttct gattttaaca ctgtatgtta
     3001 atttagtcaa cgcagttttt ttaaaagttg ttaaatttgt ttacgaatat aaaagaaagg
     3061 agagaagaat atggaaaaaa cagtctcggc aaaaatcgaa gatttgaaat tagacatact
     3121 gaaacaagca aaagtggcga tggaacacgc ggtagataaa gaagactctg ccatggttgc
     3181 agccatagca gagattttag ctcacgttta gtcatcgttt ttacggtctt ctggaagctg
     3241 accatagata acagcataat gaatatctaa ataagcttga gcaatttctt ttggcgaaat
     3301 tgattcgcct ttagtagata cttgtgattc gtgatttagc catgcaacaa ctaaatccgc
     3361 agcgatttca tcagtttttt tattcatact tattcaccac cttatgcatt atttcagtag
     3421 accacttact gataagaaaa ttatatcaaa gaaaggaatg aacaaaaatg aacacaccac
     3481 aaatttttag ttttgaacaa catgaagtaa gaacgtttct agaaaatgac attccgtatt
     3541 tcgtagcaaa cgatgttgcc aaaactttgg gatacaaaaa tcccagtaaa gccaccaatg
     3601 atcattgtaa aaaatcaatt gaaacatggg gtaacgattc gttaggtcgt cgtcaaaaat
     3661 tcaaagttat tccagaatca gatgtttacc gcttgattat taaatcgaac ttaccaagcg
     3721 ctgaaaaatt tgaggcctgg gtaatggaag aagtccttcc aacaatcaga aaaacaggta
     3781 gctattcaaa cgtacctcaa agttttgcac aagcattgcg tttagcagca gatttagaag
     3841 aaaagaacca attactcgaa caacaaattg ccgagtacga accaaagatt agctacttag
     3901 atacgattct ttcatcgaca gatacggtag ctacttctca aattgcagct gattacggaa
     3961 tgtcggcaat tgctctaaac aaattgctaa acgagttagg tgttcaacat aaagttagcg
     4021 gacaatggat actttaccga aaacatatga accaaggata cacaaaatcg cacacaagtg
     4081 agattccgaa agccgatggc ggcactaaag ttgtaatgaa taccaaatgg acacagaaag
     4141 ggcgagtgtt tatttacaac ttattaatcg cagagggcta ttaccctcaa atggatttag
     4201 aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga
     4261 tagagaaaaa atcaaagaat atgtcgaaag ttcgaagttc ttaacttaca ccatgcttgc
     4321 tgaaagattt ggaattagca aaagctactt atctttaatt ttaaacggta aaaagacttc
     4381 tgcagaagca aatagaatca tagattcgat tattactatg tacgaattgt aaagggagga
     4441 acagctaatg caatatctag aagcgaaaat cccagttcca gaaggctatg taattatctc
     4501 ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc
     4561 agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa
     4621 cccaagatat agaaatatta tcgatatcga aaaaaataaa aatggatttg tctattatcc
     4681 agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac
     4741 aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga gcgctagtat
     4801 tcagcgcacc gctaatcatc tacgctttag gtctatgggg tagtagacaa gcattaatcg
     4861 gcacgatcgt ttacatgctt tggattttca ttgggcttga tgaagctgag tacagagcga
     4921 aaaagccagt cgggagggac tgactaaaaa agtctaatgt ctataagaaa ggtttgacaa
     4981 taaatgaaag aattattttt tttaataact ttcatcattt tttctatcag tgttttctac
     5041 attaaccttt gcgttgctag atttgctttg aaaagtttta ggaacagcaa acgaaaatag
     5101 agctataaaa gatacgaatg tagtagctac gttaaattgc tctttagaaa acgtcaatgt
     5161 cattggaatg ataattgata tcgatgagaa aatctttgcg ataataacta tccatttttc
     5221 aattataaac atttttaaat taagcaccat tagacaaaat gtagttccta tatagaggat
     5281 aaatgaagat agaaataaac atattagtcc agttttaaaa aaagagaagt aatctatgtg
     5341 ggcaaattct gttaatatgc ttttttgatt taacacaatc aaactactgt tggacaaaaa
     5401 tgacagtaag gaaagagttg ctccgattac taaataaaaa atagttgtta acaatattat
     5461 attatcaatt tttctgctca tgttttcacc ccaccagaat tatatcaaaa aggagagaag
     5521 aaataatgca agaattagta attttgaaaa ataaagaagc tgtgactacg agcttacaag
     5581 ttgcagaaag cttcgataaa aagcacagac atgttttgac ggctattgat gaactaaaag
     5641 agggggttgc cgaaaattgg gcagacctat tttgggaaga tacttatgtt catcctcaaa
     5701 ataaacaatc ataccgaatt atttatatga atagagacgg cttctctcta ttagcaatgg
     5761 gcttcactgg taagaaagct ttaagtttta aacttcaata tattgaggcc ttcaacaaga
     5821 tggaaaaaga attaaaagac caattgccat caattccagc aactaaacga gaaatggcat
     5881 tacttgctct agctgcaaac gaagaaacga atgaaagagt agacgctatc gagtctgatt
     5941 taaacgattt gaagaacaat caacttctag ctgaaccaga ttaccgaaca atttctaata
     6001 tggttcgcaa caaaattaga gttatttgta atcagcagca tttaaatagc aaagcaaaag
     6061 cagaattgtt taaagacttg aatggcggaa ttaagcgaat cactggagca gtagctagaa
     6121 atcgtatcaa ggcaaaacag ttcgatgatg ttatcgattt tattaacaat tggatgcctt
     6181 ctacagcgac gatgacaatt attaagcaaa tggagttgtt agaggatgaa tagagctgaa
     6241 gcgctaaaaa taggcgtaat aattgctaat cgctggtgga gacacaataa accaaacatc
     6301 ctaagccaac aacatattga taagcaaaaa gcatggcaac aaataaaaaa gtgactcagc
     6361 cgaccaaagc aatgagtcac aaagaaaata catctaagga gatgttacca catgaaaaaa
     6421 gaactttcca ctctagatca atatttgact gatcctagtt ggggcaaatc gaatatcaag
     6481 gaaacaagca atcgaaaaat cagacgtaat cttttgacgg atgaagaact agcatgtgat
     6541 caagacgatt tgggaaattt tgtgagtatt tgggatcatg tctatcttat ccatttatcg
     6601 aggaagtcca aaaaaactga atatatctat gtcatcgaag atggcttgat tgatgcgcta
     6661 gaagagtatg acagagataa cttgattgat atctcttatt acggatcagg taagaaatac
     6721 attgctgaaa tggaggcaga atttgatgag tgaaaaggaa caacctttga aaaatagaag
     6781 tgataatacg ctttttaata ccttatacaa aataaatgtg aaagatgtta ctgaaaaacg
     6841 aaataacctc acttatcttt cgtgggcatg ggcttgggca gaagttagca aagtgtgtga
     6901 ggcagtagat tatgaaatct atcatgatcc agaaacatat ctgccgtatg tctttgataa
     6961 gaaaacaggc tatatggttt ttaccagtat cacagtcaac ggagtaaagc gtgacatgtg
     7021 gttaccagtc atggatggtg caaacaaggc aatgaaagat gagccatata cctacgaagt
     7081 caatgattat cagtggaata acgaaacgaa gaaaaaagag attgttggaa aaatcgaaaa
     7141 gcgagttgaa gcagcaacta tgtttgatat caacaaaacg atcatgcgct gtcttgtaaa
     7201 aaatctagca atgtttgggc tagggctata tatatttgct ggcgaagata tgccagaaga
     7261 tgtctcgatg cttgaaccag ctactcaaag aagcaaaaag ctattcttgg atgctttaca
     7321 actggttgct aacaagtacg aaaaatcaat tgatgaagca attgttgcat tgactgatgc
     7381 ggcttctata accgctgatg acagtaaatg gaccaagaga gacttgggca ttctaaaacg
     7441 aggcgttaac tggattgaag atcagtacag agaagaaaca aaagagaagt gatatgagtg
     7501 tttaaaccat tgatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata
     7561 ggtgcaacga tcaatgaaga agtgaacatt gatcgactaa agacgatgta tgacggatat
     7621 gatggcgatc ggattattga agttcgattt attgatccac gtcgatttac tgtgcagcaa
     7681 cgaaacttca tctatgcgct gataggcgat attttcatcg atacaggcat gccaacggac
     7741 ttctggaagg aattctttta cttccgtttt gaaggcgtca cagggcgcaa aataagcctc
     7801 aaagacgaat cgaatacaac tgtgagtgat gctaatgtct tagcaaatat catcttagat
     7861 ttcatctttg aacatcatat tcctttcaaa gaaggctatg agattttacc agcgaatcaa
     7921 gaatattact tctacaaatg catcacaaaa agagtctgct gcatctgtgg caaaacagga
     7981 gctgatatcg atcactttga caaagcgcta ggaagacgaa agcgcaaaga agttgatcat
     8041 tcagagtaca catttgcagc actctgcaga atccatcaca cagagaagca caaaataggt
     8101 gtgattaatt tcaaaaataa atatcaaatc aaagggatca aattaaacca gaaaacaatc
     8161 aaaaagttaa atattggagg gtaaaaatga cagaacatcg aagttattat gcgattatac
     8221 cagccaacgt aaggtacgac aaaagactta aaccaaatac taagttgtta tacggagaga
     8281 taacggcctt gtgtaatgaa agaggctttt gttgggcagg caatgagtac tttgcagatt
     8341 tatatggtgt gaataaagag accatatcgc gatgggtaag tgatttgatt aagtttggat
     8401 acttgaatcg ggaaatcatt tacaaagagg gtaccaatca aataatcaat aggtacctac
     8461 gaattaatca ataccctatt gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag
     8521 ataataatac atctattaat aatacattta ataatacaaa agaatatata agagagttac
     8581 cgccttcgaa aaaatcgaag gctaagcccg tccgtcataa atacggagag tataaaaatg
     8641 ttcttttgtc agatgagcag atggagaaac tcaaaacaga attccctaat gattaccaag
     8701 agcgaatcga acgactgtca gagtattgtg aatcatctgg taagacttat aaaaactatt
     8761 tggcaactat tcgaagttgg gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg
     8821 gatacaagcg cacaggaaga cgagagaagc ttcctgaatg ggcaatcgac caagaagcct
     8881 atctcaagaa aaaagcgcta gaacgagcta atagacaatc aaaagcacca ttctaagagg
     8941 tggaaaaatg aagatcgatt atctagaact aattaatgaa atagcgaatt ataaaaaggg
     9001 cgaggaatta gacgtcctga gagacgtata tgatcaactc gaagaagctg gaattgaagg
     9061 aattaagaat gatcgttcga gttggagtaa gctcagatac tatttcgcac tttatatcga
     9121 tacaacacaa ttaagaaatt tagcatatac caaattacta tttgttgatt gtgttaaagg
     9181 cttgcaaaaa catcttaatg aacttgagca ggtgtaatca gatggaccta aagacattta
     9241 cagcacagat tgaactaatg catcaagaag ctttaagaca aagtgtatcg tacgaagaca
     9301 agtggctcaa cacgttccat ggcggacgtg agagcgcact tgatcaagta ctcaaattac
     9361 tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact
     9421 tattcagaag attggcaaaa tgacaaagaa gctgcttcag aagtgatgag gcttggaaga
     9481 gcgatgtggg cagacaagag caacaagcca agaccacgaa aaatcgcaat ttggcacggt
     9541 gacaaacttc tagtgatagg gacagctgaa cagttagcaa gtctcacagg cttgcacgag
     9601 aaaatcgtga ggaaaagagc aaggtgtggc tacacagacg ctaagaagag aacgtttaga
     9661 tacgtggagg aatcatcatg acaacagaag aagtgattca aatgcgtatt cgaagccttc
     9721 agcgtgagat tgacgatctg gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa
     9781 aggcaatcga tttgcacata gagaatttaa gaagggaaat ccatcgattg gaggaatgag
     9841 cgtggataag aaagcagcaa tgaaaagaat tgctgaatta accaagtcag aatcttggca
     9901 agaagacaaa gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa
     9961 gcccaaacgg agaacgccga gaaaaattgc aatctggcat gatgaccgaa ttttagtaac
    10021 aggtactgct gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag
    10081 agctaggagc ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata
    10141 atggatctca ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag
    10201 aaagataaaa aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga
    10261 gcaggagtgc gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac
    10321 gaaattcaaa aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag
    10381 aaacttcatc aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat
    10441 ttgaggagga cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat
    10501 cgaatcagga gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa
    10561 gcgccagatt attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt
    10621 cgaagaatca aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa
    10681 atacgaggag gacagcgaat gataccgaag tttagagcgt gggataaacg aaagaacgta
    10741 atgagagatg tagccgtctt gcattttact aaaaacggca aaacaaactt tattgaatat
    10801 tggataaatc ctaccgaatt gaaatcatat cacgtgcgaa acatcgacct catgcaatcc
    10861 acaggaatga aagataagaa tggtgtggat atatttgaag gggatatagt attagtcagc
    10921 gtgcgaaatg gcttcgatta cttagataat aaagtctgta ttgtcaaaaa ttcaatagat
    10981 tattccggat tagtttgtgc cactgttgat gaagacttag agtatcaaat ttttaacaca
    11041 gagctgtttg aagaatacac gtacgaagtc accggaaata tatacgagaa tagcgagtta
    11101 ttggaggaac agcgatgaat aaacaggaag caatcaaaaa gttagaaagt attaaagcga
    11161 taggaaatga tgcaatagct gcttgctata acgagagtat aaattcaggt attacgttaa
    11221 tgaaaaaaat agacgaaccg cagaaaccag ttattccaca actcgtggcc ggttggcttg
    11281 agaaatctac ggaccctttt acaaaagctg aaaaaatagc gtatttaatc aaatctaaag
    11341 atggtgattc atattatttc tgtgattggt ttgtacgaga tggcatagtg acgcaagagc
    11401 aaggagaaga attactcgct tgggcaacaa gacaatcata tgaaacacta ttgagcctat
    11461 acaacggcta cgaggttgag aaagagccgt tatgggcaat aaagaatgcc gatggaaact
    11521 atcttactaa atgtgcttta tggggaaaag atggagtaaa ttatagtttt gaatgcaatc
    11581 catctcatcg attgcttttc actgataaag caacagcgga tgctgcagca ttgttggtga
    11641 atggaacagt ggaagaggtg gtagaaagat gaaactaaaa gacggatttt acgctagcag
    11701 tcacggtatc ggcggtttaa tgctagatat gccgacaaag aaccctaaaa cacgcaagaa
    11761 atcaaaattc aaagtcggtg acatggtccg ctgtgaagca gaagagttcg tttatccgtt
    11821 cagaggatac gttaaaaaga tactgtcaaa ctcagcaatc attcgtattg aaaatacaat
    11881 gaagtgtgat cgttcgactg caaaacacaa gcattattta gctgtagcga gattagttga
    11941 catggaagta atcaagagca aataaaaaaa gccggatcgc tccgactgat tcaataaatc
    12001 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
    12061 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
    12121 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattataacg gatatgccga
    12181 aggcaccgaa gcatggcaat aaggcagaag acgcgattat tcagatgatg gatatagaaa
    12241 cggagagaga tgcgattcta gcggctttga tggctcttag tctgattagt cgtcagatac
    12301 tctactacag cttctgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg
    12361 gttattcaga aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag
    12421 catataaaca cggaaaaata attgcttata aataatttgg cggttttttg gcggaatgat
    12481 ggcggttttt agacatttac cagtgttatt atggtagtgt cgaaagatta ggaaacagga
    12541 tcgacaaaat aaacgaaggg aggcaatctc cctcatcgtt ttaaacttct tcttagatag
    12601 acagcaacta attatgaaga aaaggatgtg aattcaactc ctcctaaatt cttcatcggt
    12661 tgctgtttat tttgattaga cagcagcact cacaaaacta aacatgttct tgtcgtcttt
    12721 caactgctgc tgtctattaa tttatgtatt ggagggaaga aaaatgaaat tatcagtaga
    12781 aggcactcca gaagaaatac aagaattgct ccaagctatt ggtagtagca aggagcaaaa
    12841 ggtaagtgtt tctaattcag atattgacgg tatttgcaat tgtttaaaca gagaaaaaat
    12901 atctaatcct tactaagaga aaatatgttc gttaatgaat ttccaatttt cctctgtagc
    12961 tctaccttga taattttttt gcagttcaca gatgaataag gaatcgttat tatcaagtac
    13021 tgtatttaat ttttcacaca tttgatttgg attcaaatta gttttaacta accaaaaaga
    13081 tttttgtagc tttatatatg ccccaaattc ttttattatt tcaaatactt tatcatactt
    13141 ttgccctggg ttattaagat cataactaat catgtagggt ttatacatgt tgtcttgcac
    13201 atccttgttt tatatttcag cggaccactc gctgataaat aaaattatac gcttagtatt
    13261 tattttcaca atattaattt atcgtaataa cttttgagta aatagaacaa aaaacctgca
    13321 ctagtttccg ctagtacagg cagtgactat atcggttgat aatctagcat atattaaaaa
    13381 taattgcaag aaaagaaatt tatagtggtt tataccaaat tatcaaagca atgttatttt
    13441 gttgctgtct attgtttttt aattattcac acgataacta aaggtgggtg aagagaaatg
    13501 attccattaa taatttcaat ttttgcgctc tgtcttaatg tctatatgat tggatttaaa
    13561 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
    13621 aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat
    13681 tccttgccag cgacattaaa tgcctatgac ggttacgact accgaaaaaa gatcgttaag
    13741 aagctatacg gtgctacgta cggcaatgta gtaagtgtgc tatctgtgta caccaccaag
    13801 cttcggtcac tgtggcggaa gtagaagacg cagcggtaaa tggcgagtag cctcgtgaga
    13861 gcctggtaag ttctcgtgag tggtgcaatc ccactccagc gactttaagc aaccgaggca
    13921 tcggcggttt aaaaatatag gggtgcgcaa tttcgtacgc gttttgtgca tcgtgcaagt
    13981 tactattaca tattagatca ctctttgagt ggtcttttta tttttgcaca aaggaggaaa
    14041 caacaatgta tagaccacaa tacttagaac agaagtatga agtaatcact gttcataaag
    14101 gtaatggcga aaaagtatat gagtatagaa gaccaataaa gagcgataca tataaacgaa
    14161 aggaaaacaa tgaagttatt ccattgtatg gcaaaagaat agctaagcat taaataagat
    14221 tgcgaaagga gacggaacat gaccgaggaa ttctatagat ggctattaca gttgacaaga
    14281 gaagatcgtt tggttaagtt ctatcagtct cctaaatggc gcaggcttag agagaaagcg
    14341 atgaaacgag atcactatga atgccaagag tgtagaagac taggtaagta tcatagagta
    14401 gagaacgttc atcatataaa ggaagtcaag gatagacctg acttagcttt agatttagat
    14461 aatcttattt gtttatgtgt tgaacatcat aatgaagttc atggcagata tcttacagca
    14521 ttagataaac aagagaagaa gatagaaagc tttgctaact tcgatgcaag tgaaaggtgg
    14581 taagtgcatg atcatcaatg ataatggcag agagtatgat acagaaaaga ttgaagagta
    14641 ttcatcttat actcagggat taattaaacg tttgatatac gttcgctatg taggtattag
    14701 ggatctgtta tcagataact gttgtagtaa atacaaagtg aatcaagtaa gagaagcgtt
    14761 gaataaagat aataacgtcg aaagaataaa aaatgttttt ggatatagta ttgaagagat
    14821 taattattac attgacttcg ctgaagcttt cattccgatg gtgagataac ccccccttaa
    14881 aataaatcgc aaattttttg ggggtgatga aacggagggg gctgtcagga aaagagattt
    14941 tttcgaactt tatcatgaaa ggagggctaa aatgtttaaa aacgaattgt ctcaaaatcg
    15001 ctacagagaa aaattacgcc gctctttaat aagccaattg gaaagtcaga aaacaaatat
    15061 tgagccattc ttagataatg ttgatcgtta tatcagttta tgggaaacgg cgatatcact
    15121 ggaagaagat atatccgaga acggcattag actggagaat ggtaaaaaga atgaatcagt
    15181 agcgttgctt gtttctgtca acaaacaaat gggattgatg ttggataaac ttgccattac
    15241 tcctgaattg gtaggtgaag caaatgaatc aattcctgag ttataagcat attgaaaatt
    15301 ggttcaaagc tatagaagaa ggcactatca aggtatgcaa agagcaatta ttgctaaaaa
    15361 attatctaga agaaagagtc tttactagag aagatattta cttcgataag cagatggtag
    15421 aggattcaat caatatacca gcacaatact ttccattcga attaattccg tgggaaaaat
    15481 ttctacaatg ttttatttat ggtgttcgat ggaaaaaaga taaaacacta gtgttcaata
    15541 gatatctttc attaatggga cgtggtaatg gtaaaactgg ttttgcttct tggaacaact
    15601 tctttctact aaccgctaaa cacggtatta aaaattatga tattgatatc tatgccaata
    15661 atgaaagcca agcaaagact agttttgatg atgtatttaa agtaattaaa gatcatcctg
    15721 atttagataa aaaagtattt aaagctacga aggaagttat tcaaaatatc gctacaaaca
    15781 gcaaacttcg ttataacacg gcaaatgcta gaacaaaaga tgggaagcga ccaggtgcaa
    15841 accgctttga tgaaattcac gaaaatgaag attattcaat gataaatgtg gctacttctg
    15901 gtggtggtaa aattcgagat tatagagaat tttatgatac aactaatggt catgttcgtg
    15961 gtggtccgct tgatgacatt atagaagaat caaaaatgat tctttctgga gaacttggaa
    16021 ttgacaagga tggagcagaa ttttctagtt tgtttccatt tatttgtcgc ttggataacg
    16081 ataatgaagt tgatgatccc gacatgtggg aaaaagcttg tccaactatt aattacaatg
    16141 cagatttaaa acggaaaatg tttcaagaat actctcaaat gcaacgtaat gctggtttaa
    16201 gacttacgtt catgaccaaa cgaatgaaca gacctatgga agatacacga tttgctgttg
    16261 cttcatatga tgatgttctg catacgaaag aaaaagaatt tcctgaaaaa atggatgaag
    16321 tgataggaac agtcgatttt gctgatagac gagattttgc cagcgttggg ttgctaggaa
    16381 aatacgataa agatgtttat tttacacaac atacttttat ccacgaatca gcccttcgat
    16441 tacaaaacat caaacgagag gttatagata tttctataga tcaaggaaaa tcacagatcg
    16501 ttcatggaaa aaatatagaa gctgattata ttgtaggttg gtttcttgaa atgagtaata
    16561 aatattatat taaaaaaatc gctatggata tgtaccgtgc aaaaatattg aagcccgctt
    16621 tagaagaagc aggttttact gtggaaattg ttcgaagcgg atctgttaca catggtatgt
    16681 taaaagatct ggttgatgac ctttttatta atcaacgttt attttttggt gacgatgcga
    16741 ttatgcgttg gtattgcatg aatgtatatg aagagcatat ttctaatgga aatatacgct
    16801 atgaaaaaat agaacctgaa actagaaaaa cggatggctt tttttcattc cttcatggtt
    16861 tgaatttttt agatgatatt tatgattctg ctcctgtaac agtcacaaat agctcagtag
    16921 aaaatacagg aactggattt actcctctag tattctaact tgaaaggagg tgagaaagtg
    16981 gggatttttc aaaaggcggt aggatacttc acaaaaaaag caacggttcc tttagaagaa
    17041 tacttttgta aattgcaagt tgattttgtg tatcgaaaat ttgcgattga aacttgtatt
    17101 gatttgattg caaatgcgat gagtaaagcg gaattcaagt catatgaaga tggaaaaaat
    17161 aaaaagaatg atctttacta taggctgaat gtagctccta ataagaaaaa taatgcaaca
    17221 gaatttagaa aaaaactgat caggagatta atattctaca atgaagtatt gatcgtttct
    17281 ccgtctaata attctagcga aatatttatt gcggatagtt gggatgtcac agaatatgca
    17341 ttgaaagatg atgtgttttc tcaagtgcaa attaacaaca tagtccttga tagagaattt
    17401 ctagaaagtg atgttatcta tataaaatac gcagatcaac aaattaggca actagtcgat
    17461 gcgtattatc aagcgtatgg gaaactcatt tctagtgcca tgaatgttta caagcgctct
    17521 aacgctcgta gatacgtact gaaagggaat ttattccgac cgcaagacaa tacaacacaa
    17581 gatcaaatca ataaaatgat gacatcacaa tttaaggctt ttatggaagc tgataatgca
    17641 ggtgcggtat ttcaattaca aaatgagtac acattagaag atttcagcgg aaacttccaa
    17701 agcaattcaa gagatataaa aaacttaata gacgacatct ttgagatgac agcagcagcg
    17761 tttcacgttc cgaaaaacct actaaaggga gacatgagtg ggttatcgga tcaagtggac
    17821 gcttttttaa tgttcgaaat cataccgatt gctgaactta ttcaggatgc gtttaacgct
    17881 agtctctatg aagtagaaga atacttgtca gggaattttg tacgtgtgga tacaactatg
    17941 atcaagatta ctagcttcaa agatttggtt gacgctattg atgtaggcat tagaaatgga
    18001 gtatttacaa tcaacgaagg aagagaacgc gttggaaatg atcgctctga taaggcgatg
    18061 gcagatgaaa tatttataac taaaaacaat caacaagtat cgaaaggagg tgaggcgaat
    18121 gacgacaatg aaaacatttc tagcagtaaa gaatgaaggc gcagtaccgc aaatttttat
    18181 tcagggattt attggttcta gttggttctt tgaagggaat actgacaagg gaatcaaaaa
    18241 tattttggat agtctaggtg atcaagaaga aattgaagta gtaattaatt caaacggtgg
    18301 agacgtattt caagggattg ctattgggaa cttacttaag tcaaataaag caaaagttaa
    18361 cgttgtgatt aacggcttag ccgctagtgc tgcttcaatt atcgcaatgg ctggcgatac
    18421 tataaaaatc tacaacaatg cacaattgat gattcaccgc gcttccacat acggagaagg
    18481 aaatgtcgat gacttccgta cgattgctga ccaactggaa tcaattgata aatcggtaaa
    18541 ggcttcatat aaaacacgat tcaatggcac agatgaagca ttgcaagaac ttcttgaaaa
    18601 agaatcgttt atggatgcag aaacagcttt gagttatgga ttggtcgatg aaattatcga
    18661 tgcagaaaat agctcaggta ctgaagctaa aaaagaacaa agcgttgaag aaattttgaa
    18721 tgacgttaaa gaaaaaagag cagaaaaaat tgctgcattt acagcagcat taaataaaac
    18781 atttggacaa ggagatgtaa aataatgaca gttaaaaatt taaaaggtgt aacagctgca
    18841 agcgaccaat tgatgaaagc ttttaaagat ggtaacgaag aatcttttag cgcagctatg
    18901 gtaagcttat ctaaggaaat tcaggataaa attttagaag aagcaacagc aaaaaatcaa
    18961 gatcaattag tattaatgaa ccgtggtcag cgtgtattaa ctacacaaga aacaaaattc
    19021 tataacgaag tagtgaaaaa cgaaggtttt gcaggggtcg aagaattagt gccagctact
    19081 gtatttgaac gtgtatttga agatttagaa caatctcatc cactattgca aaaaattact
    19141 tttgttaaca caactggtgt aacagaatgg attgtgtcac gtggagtcaa tccagcatgg
    19201 tggggtaaac tttgcgaagc tgttaaaaaa gttttagata atggctttga tgtaattaac
    19261 atgaagcagt tcaagctatc aggttatatt cctgtatgta aggcaatgct tgatttaggt
    19321 ccagtatggt tagatcgtta tgtccgtact gttttagtag aatcattgag aattgcatta
    19381 gaacaagcaa ttgttgatgg tactggtaaa gatatgccag tcggaatgat gcgtgacatg
    19441 agcaaacaaa ctagcggaga atatgctgaa aaaaaagcag aacctattac agctttagat
    19501 gctgtaacta tgggcggttt gatggcgcga ctatcaaaat tcaatatcga aggtgtgaat
    19561 gatccgattt atcgtaatgt gaatccttct gatgtggtcc taattgtgaa tccaactgat
    19621 tactggtcta aagtattccc agctaagact gtactaactg ctaatggaga atacgtacaa
    19681 gtattgccag taccagtttc agatttgcag tcaacggctg tgccagaagg aaaagcagtt
    19741 attggggtag cctcagatta cttcatgggt gtaggatcta cactaaaaat tgaagcttca
    19801 gatgaatacc attttgttga agacgaacgc atttatctag ctaaacaata tgcaaacggt
    19861 caacctaaac gtaacgatag tttcattgta ttagatatta gcgctttggg aactactact
    19921 acaactacaa aaccaacaac cacaacaact acaacacaag cgtaggtgat cagaatgaag
    19981 tatattcttt gtcagccggc aatcaatcgg tttaaatggg agcttgaagt ttgtttaact
    20041 aatctgaaga aactaggaat caaagatatc gtattgcttt tcagcagaca cgatgatcag
    20101 attcctattt tttttgagaa ggaatatggt gttgaagttc atgtgtacga tgatctgcgg
    20161 gacgacaaag agtatattcc ttcgattaaa ccatatttat ggtggaaata tttagaagaa
    20221 gatcattcgc gtgaggacga ccgatatttc tatatcgatt cggatgtcat tttcaataaa
    20281 agaattaatt tgcgcaaatt gccttctaaa gatgatgttt ggtattgtag cgactgctgt
    20341 agttatctaa gtcttgatta tattagaagc tgtgaaaacg gagaaaatat tctaaaagat
    20401 atggcaaaca ttgtaaatgt tacagtagaa tctttggaaa ctataaacac taattcagga
    20461 ggcgcacagt gggttattaa ccgtcctaaa gcgaattatt ggaaaaaggt ttatctggat
    20521 tctaatcggc tatatcgcta ccttagaggg caaaaaacaa atatacaaat ctggacagcc
    20581 gagatgtggg cacagctttg gaacatgatg tatttcaata ttggtcctaa agttcacgag
    20641 gaattagact tttgttttgc tactgatcca atagaaaaag ttaaagaagt aaaaatctta
    20701 cacaatgctg gagtaacaac aaacgatgaa gatttatttt tcaaagggag atacgtgact
    20761 tctacgcctt ttgatgaaga tttatcattt gtaaacaaga aaaaatgctc ttacgcatat
    20821 gttaaagcaa ttaaggcggt ggttagatga cgcctgaaca agtgactgaa gaattgctaa
    20881 tagctgtgaa ggataatatt tacgttacct ggaacgaaga agatgagtca attaaaaaga
    20941 tgatagctaa aaatgctgtt tatcttcaaa gtaaagtgag tacaacactt tctttttctc
    21001 ctgaaagctt agaatacgga ttgctaatcg aaagatgtag atacgactgg aatcgtgctt
    21061 tagatgagtt tgaacaaaat ttcgctagtg agttattagg tttcattcaa cattatgcgc
    21121 tacaagaata tattgcaggt gatgtgaatg gcgaataatc gtagactcga agaaacattc
    21181 aacgatggtt ggttaaagat tttgacgcaa actaccaaaa gaaatgaact aggaaaaaag
    21241 attggtgtag aagatacaga aatcacttct ttaaaattta gaaatctttc catgagagat
    21301 agtgatataa cagctatgga tgcgatggga tcgaaattaa ctaagaaagt aaagactcca
    21361 tttcatccaa tcgccaagaa atttaataaa gatcaatatt ttatcgtaat cgatagtatg
    21421 cgttacaacg ttatctatgc cgattacgat aatttttata tctattttta tcttgaaagt
    21481 gtgggtgaat atggtgattg ataattctaa agaaaaagaa cgtttaaata agcaaatttc
    21541 tgctatcaaa acttccttag aagagcattt tggcctcaaa ctctttcaag actccgtagg
    21601 cgaggatgag ctacctgatg attttaatta cttcattctc gaaacaggag aaatagaaat
    21661 gatcactgag ccaaaatata gcgtgggtca aaatctatat ctaactttct attcagaaaa
    21721 tagagaagat ttaacaggag actcactaga tattatttca ttgattcaaa atcgttcgat
    21781 tcgttttcag agaatggatc ccaatcattt aaaactagag aaccaagatc gctatatcga
    21841 tcaattggta tttacgttta gacgattatt gaagagtgat tgtcatggct aaaaatagtt
    21901 gggagctaaa aataaatgga catgatgaac ttcttgtgcg gatggaacgc tattcaagcg
    21961 agagcgaacg actgattaac gaagcattga aatcaaaagg ttcggctatt gcagtggata
    22021 ggattacaga aaaaattcct gtttctgaag cagatttaag aagagggcac caacacgcaa
    22081 aaaatagtcg tccacttaag actcaataca ttaatttggg tttcatcatt agacctacaa
    22141 gaaaatttga gtatttaaaa tatcctgatt tggggatagg tacttctaaa agaaatcagc
    22201 cggacgaatt tatgagaaga ggattaggtc ttgcacttga tccaattaca gaacttctga
    22261 ttcgtcaatt cgataaatta aataaatagg gggaacaaca atggctaaaa caaaaactgt
    22321 agtaacaacg ttcgataacg taagtatcaa acgaattgct tttaatttta agaacgcagg
    22381 aaatgcaatc gcaacgaatt gtaatggaca attagatggc gaaacagaaa tgcaaacggt
    22441 ggttaaaaaa tgtggagcga cagaagtaaa atcaaaatct aaaccaatca atatgacggt
    22501 aacaattact gcacatgtac cgatggaagt ttatcgacgt ttcaatgggt tgaaacaaga
    22561 tggacgtatt aaaccaggca tttactctta cggtcctgat tccgtaggcg aagatttctc
    22621 acttgctgca gagatcgtgg atgacttcga agaaaataga aagttagttg gtatgttagc
    22681 atgcacttcg aatacaggat taacattctc tattgaaaat ggtgcggatg aagtagctgc
    22741 gttagaacta gaagcaaaag ttatgcaaga tgaatttggt aaattttatc atgaagcaat
    22801 tgttgcagaa cttgaagaag acttaacaga tcaatggatg acgaatctat ctgctgatgt
    22861 gattaaaaaa acttcagttg tgacaactac ggccactcaa tcacagtaaa aaaaacggag
    22921 gtagcgaaat gaacgaagat tactcaaaaa ttgaactaaa cgatggaaca attttgaatt
    22981 tagaacctaa actgaatatc aagaaattat tgatgatcaa tagagatttt aacacagacg
    23041 agtttgcaaa aatgtcgatg ggaaaaggct ctatggatat tacagttatt caaggtgcaa
    23101 aagccgtata tgtagcttat cgtcaagcga acatggtcga ttacatttca ttcgatgaat
    23161 ttatcgataa atgggatttt gatatggagg ttgcagtagc tgtatacagt actatgatgt
    23221 tcaaacaagc acgtgatgcc tatcaaaaag aattcgaaaa agcgaataag gaaaaaaagc
    23281 ttcaaaagta aaaatgccaa agctcttagt tgaaacgtgg gtcgatgtct attcgatgtt
    23341 gaccgacgtt ttttctatgc cttcagattt ggttttaagc gatatctgtt tagatgacat
    23401 tttgcaaatg gcttacaaca agagcgctta tgaaggatgg aagaactatg caataaatca
    23461 atcccagaaa aactaaagga aaggaggtaa aaaatggcta aaaagagaac ggaagcagaa
    23521 gtaactttca tagctaacga tgacggattg aaatctacgt taaaagaaat cagtgctgaa
    23581 ttaactaaaa atagagcaga attaaaacta gaacaagctc aattacaaca gactggttct
    23641 gaatcagaca agttaggaag taaattatct tctttagaaa agcagtatga attacaaagt
    23701 caaaaagttg aagtaactag tcaacgttta gccaatgcca aaaaatatta tggagaaaat
    23761 tccaccgaag ttcagaaact tgaaagagaa ctgattaatc aacaaacagc tcagcaaagg
    23821 ttatctaatg atatagataa aacgagtaag gcattggctc aatctaaagg tgaatttaaa
    23881 acgtatgagt caactatgaa agacttagat aatgagcaac aacatctaaa gtcaagcgca
    23941 tcattagtag aatccgaata taaaaaatgg caagcaactg ctggccagtc tgctacagaa
    24001 tctgaaaaat tagcaaaagc acaagaatat gtcggcaaac agagtgatat tgctgaacaa
    24061 aaaatcgaag tgctaaaaaa acaattagat gccacacaaa aagaatttgg agaaacatct
    24121 accgaagctt tagaaatgaa aactaagctt aatgatgcgg aaagagaatt cgaagaatta
    24181 agtaatgctg ctaaaaacgt tgatacctcc acagtagatg atatcggcaa aaaacttgat
    24241 atgggtaatt taatggaagc tactgatcac ttgtcagtga tcggagataa gcttattgat
    24301 gtaggtagta agtctattga agcagctgga aaagcacaag ctatgcaggc ccaatttaaa
    24361 caagtctttg gctctttaga aggggaagca caggacgccg ttgagggaat ggctgaagaa
    24421 tttggaatgt taccaaatac gatcaagcct gtttttacac aatatacgtc aatgtttaaa
    24481 ggacttggat atgataccaa agaagctatg gagttagctg gtgatagtac tcagttagca
    24541 gcagatgcag cagcttttta cgataagtct atggatgatg ctagtgaatc tcttaattca
    24601 tttataaaag ggaactacga aggtggggag caaataggtt tatttgctaa tgatactcaa
    24661 atggcagctt atgctgttaa gcataatttg ataccagcga ctgaaggagc aaaaaaagcc
    24721 agtgaagagt cattgttagc tgttgaaaaa gcacaatcta agtatgctga tgctattaag
    24781 aaacatggtg aaggatcttt agaagcaaga gaggctgctt taaaacttaa agatgcgcaa
    24841 gataaaataa atgaagaatt aggcccacaa acgcaaaaat ggtctgattt agatgaggct
    24901 accaagcaag cagttcgagt tcaatatgct gaagatatgc aaaaattagc cggtgctaca
    24961 ggtcaggcta gtagggagtc tgatggttta gaaaaccaaa tgacaagagc aaagcaagcg
    25021 ttagaagatt tttatgcctc attgggtgaa gatatactgc ctgtgtttat caaagggttg
    25081 caagcaggag cgaaagcttt gcaaggatta gccgaatggt ggagtaaact tgatgggcca
    25141 atgaaaaatt tcattttagc tcttggagga attctagcat tattaagcac attagctcct
    25201 gttataaccg cagttgttac gatagttggc acatttggtt ctacagtttt gctgccaata
    25261 ataggaatta tagcgggcgt tgcagctgta atagcgattg taattacagc gtttcaaaac
    25321 tggggcgcaa tcactgactg gtttagtgat ttatggaaaa aatttaccga ttggttaggt
    25381 gatacctggg aaagtataaa agaaagtgcc tcatcagttt gggatggggt taaagaaacc
    25441 tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga
    25501 gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagagact
    25561 tggcaagcat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg
    25621 attatttggg cagacattgt aggaattgtt caaataccat ggacattaat aacgtcattg
    25681 attcaagctg gtattaatat tatcgtgggt atttttgatg tagctggaca gttattgggc
    25741 gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaatac ttgggatact
    25801 atgacacaat ggataagtat cgcttggaat gggattgtaa ctacattcca tactatattt
    25861 gatccagtag tggcatggtg gaatggtata tggacagcta ttagtactac ggcttcaaat
    25921 atttggaatt caattagtgc aacagcttct agtatttgga acagtatcaa gaatacaatc
    25981 actagcttgg tacaagcagc tgctacagta attcaaaata tttggtcaac tgtatctagt
    26041 tggttaggtg gaatttggaa ttcaatcagc tctacagcat caaatatctg gaatagtgtg
    26101 actagtagta taagcaatgc tataaacgca gctaaaagtg ccattcaaag tgtttggaat
    26161 agtatatctt cgtggattag cggaatttgg aacggtatca aaaatactgc tttgaatctt
    26221 tggaatggaa ttacaagcac tattagctct aaagtaaacg atggaaaaaa tgcaatttca
    26281 agcggttggt ccaatctaac aggtattgtt tccgacatat tcaataatgt taaaagtaca
    26341 attgctaaca tatgggaagg catcaaaaag actgttagcg ctccaattga ttggattaga
    26401 gacaaaatca gtggcatttt tgataatttg aatatttcta taccacatat tccgttaccg
    26461 gaatttatca tggaaggcag ctttaacccg ctaaaaggtc aaatccccca tttgcgtgta
    26521 aaatggcatg ctaaaggagg tatctttact aaaccaactt tactaggtgg aatgaacggt
    26581 gtcggtgaag caggacctga agcagtttta cctttgaaaa gatctgtttt gcaagaaatt
    26641 ggtgatcgta tcttgagtag cacatcagtt tcatctaggg cacaaacgat tcaacctgtg
    26701 aataactacg aattcaattt cacaattgat ggtaacgcag atgaggttac tatgaagcaa
    26761 acaactcaac aaatcattga tagcattaca aaagttcaaa atgataatgc ttcggcatgg
    26821 cgttaaacag gagagtattt ctcctgtttt tttagtttta aaaaggatgt gaaaaaatga
    26881 ctgattgtat acattctata atcgatggat ttcctgatta tttgcataaa ttggctttag
    26941 cggaaagacc aaccatacct tctccaaaaa gacagagagt tgaaacttct gttttaggac
    27001 ggctaggtgg cttagtacaa gattactcgt ttgaagacat gtcgtttaca ttgcactata
    27061 actatttaga agatgtggaa gaccatcaag cattcaagca atcgttttat atcatgcgtc
    27121 attggttaaa ctatgcaaag aaattagaat tctctgatga tcccaacgtc tattatgtta
    27181 tccagactat cgatattggg gatgcagaaa acgatattgt tgaatgggga gagttcgatg
    27241 taaatatcac tgcgaaacca ttcgcaagag ttcaagaaga tgtacctata accgtagata
    27301 aaccacagtc atttaacttg ctgaataata gtttagaaga aagttttcca aagattatca
    27361 tcactccttc agctacttca tgccaattca ccttaaatga ttatgtgttt agttttgaag
    27421 gcttagtagt gggaactgac atagtcattg atagtgattt gatgctttgc tacgaagagc
    27481 aatcggacgg agatatttta gatcggtcca acaaaatgaa gaccatgcaa tatccaacgt
    27541 tgcaagtgga tattaatcat tttaattgta ctggtttgag caaaatacaa atttatcgta
    27601 atgggttaag gtaggtgaaa tagatgatcg ataatttact aactatttac gataaaaatg
    27661 acgcgaataa tttagctgaa catttatatg atacgcaagg tttaggcgct ttgtcagact
    27721 ggttaacagc tactgttagc aataaactaa acggagccga gatatttcag ggtacttatt
    27781 caataaacgg aactaatgca gatttgattg tagaaggacg tattattcag tgttatgtag
    27841 atgaaaatcg agcaaaacag cgtctacgga tttattatgc aaaaacttct gtaataggca
    27901 atacgataga agtaaaagct gaacctattt tcaatgatat aagaaaatcg gtgttgaata
    27961 aatatgacag cggaacagaa aaaatcactg ctactcaggc atggcaaaac gcaaaagttt
    28021 tagcgaaacc agctatccct tcgcagtttt ctttctcgtc attagtagat acgcttgcta
    28081 atgtgaagat agaaaaggcg aattttttag aattctttgg tggaaaagag ggatctattc
    28141 tagatcgatt tcatggtgag tttctaaaag ataataacac attacgtcat gaaaaaagtc
    28201 taggtacgga tcataaaatc aaagcgattt atactaaaaa cttaactggt cttgacttag
    28261 agatagatgc tcaaagcgtt ttagttggag tttatccatt cattagcagt tcttcagaag
    28321 gagaagacga gatcactcta ccagaagaag ttattttcac ggattacgtg gatgattatc
    28381 ctgctggata tgtttctttt gttgatttta aagacaaagc gactgatgta gccacattaa
    28441 gggaagttgc taaagactgg ttgaaaacaa acatagataa acaaaaacca caagtgagtg
    28501 gttctattga attagtacca ttgaggcatc aaagaggcta tgaaaaattt gttgatctag
    28561 aaaaagtttc gatgggtgac ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt
    28621 cagcgagaat cgtggaatat acgtttaatg ttttaactaa ttcatacgat aaattagttg
    28681 taggaaacgt caaaacaaac ttcttagaaa atacagagaa taatgtcagc aatttgatta
    28741 atgatgccat tgatcaattg aaaaatgttg gcgaaatcag tgatttaatc aatgatattg
    28801 tagatcatca aactgatatg attactggtc aaaatggtgg ttatgtttta ttagatccta
    28861 aagaagcgcc tagtcgtatt ttgattatgg acacaccaga taagaatacc gcaaggaatg
    28921 ttttacaaat caacaatgct ggtattggtt tttctaaaac tggcattaat ggaacatatg
    28981 aaacggcatg gacgttagat ggcggattca atgcctcgtt tattacggct ggtgaaatag
    29041 taggaattac tattagaggt actacattaa ttagtgatgg tgctgattat agaacaagta
    29101 ttgctaatgg caaaatgact tggtactcaa aaaaagttaa caaagatatt atggagctag
    29161 aagcacgtga ttatgtaagt gctgatgccg gtattgtatc atacaccatg aaaactggtg
    29221 gtggtttcat gattagaaat ccacagggta acttggtttt tagtacgtgg gataatggta
    29281 ataacagacc gtttctatct tttggtgcgc ccaatttcag gtatagcaat gctagttatg
    29341 taacttctgg cgacggtagt tctttaagca ttaatggtag tgcgggtaac tcatgggaat
    29401 ttaaggtagc tggtaggact atgaaattta ctagtgatgg tatgctaacg ttaccaggtt
    29461 gtttttttgg ttcatgggaa gatgggaaac ttgctaggtt tgaacaatca acggtacaag
    29521 tatataaaga ttttactgtt agaggtacta aaaactcaac tgtaccaaca gaacattatg
    29581 gacaacgact attgaacgct tatgaaactc cagaatatta tttcgctgat tatggggaag
    29641 ccgttacagg tgacgatggt aaagttcgtg ttgatattga ccccatgttt gctgagacag
    29701 taaatctaag tcggtatatg acacatgtga cacctacaga actagttttg tgtgctgtta
    29761 ctcatgaaga tattgaccat ttcatcattg aaactagtaa gccaaacgta ttagttagat
    29821 ggaatttagt ggcacaccgt ctagggtatg aagatattag attaaaagag gatacagcat
    29881 atgatagcac agtgcttgac caaaaacgtt tttaaaacga agacaaggag gtatataaat
    29941 ggctagcagt ttatataatt tggctttaga tttcagcaaa gaattaaact acaccaaagc
    30001 tattatggct cgtcagggtg ataaagggat tacggtgacg gttaaaccat atttaaatgg
    30061 cttgcagatg gatacgagtg gcggaacatt tactttaaaa ggaacaacac catctaaccg
    30121 ttacgtagat aatgttgcaa ctagcgtaac tagtgaagaa gtcacgtttt ctcttgatgg
    30181 cacatttatg agtgaagcag gatattataa acactgctac gtagaatata gaaaagacaa
    30241 tcaaattcta acaacgcaag atatcatttt tttctcacta ggagtgtctg acatttcgca
    30301 aggtcaagcc gatgaatatg tttcgcaatt agaagagttg attcgaaagt ataatgaaac
    30361 ttttgatgct tttatggctg aaactaaagg tagagtggat agcttaaatc aacagattac
    30421 tgatttaact ggtcaagcta aaacgctaca agacaagtta gatgctctga aagaagaaat
    30481 ttctaagtta ggtaacttac aagtgatgta cagtaacagc atcgatttcg ggaactatga
    30541 ttatagtggg aatcctaatt tgttaagtaa gctatcatac gacttaattg aaaatcaaaa
    30601 tacttcagct ggaacacttt ctaaaggtga aaactcgttt aaatataata agatatcagc
    30661 tgaaacggaa ggtggagtag agttatatta taaacgaaga ggtatagcta actggttacc
    30721 ctctaataaa acgcttgtaa tgaccgttaa acttagagct ggagttgact atagtccagc
    30781 tgacggaaaa aaaatactga ttagatatag gtttgttgac aatggaactg gcaagattgt
    30841 tttagactta cctattaaca gtaattcgat aactcaggaa tggaaagagt ttagtattac
    30901 tggaactact ccaacatttg gcccgcaagc ataccatcct tggatacaat ttagggctca
    30961 agatgggata cttggggaaa tagaaatgag ctatgacatc aaaatcgaag aaggtccaac
    31021 agccacacca taccagccta acttattggt agaaccttac aacatgtgtc gcgaatatcc
    31081 taacgaaaat attgccgatc ctaaagttaa gttcccaatc gaatctggcg accaccaaat
    31141 atatcaaggt tacacagaag aagagcttat gataggtcaa acgtatacta tcacgcttaa
    31201 aggaacaaaa cccgcaagtc aaacctttgt agcgtataat cattggactg ctcgtttagg
    31261 agaactaaag ccggttgatg ggttgacaga cgtatggtct ctaacattca caccaacgaa
    31321 tgttgtggcg atgcctaaac ttttccgtgt ttatcagtat ccacgatcaa cagtaggcgc
    31381 atgccaaatt gactggctca agatcgaaaa aggcaacaca cgaaccccga atattagtga
    31441 gtataaatat cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga
    31501 tctagcacca gaatatgtcg aagataattt ggccacagat attaaaattt ctgaaattac
    31561 tggtaaagca aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc
    31621 ttcaattaat gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgaattaga
    31681 caataagatc gatgaacgca ttaatgatac agctactacc gcattttatg gcgcacaagg
    31741 tgaagtctct aacaaaacga aagtagcgga gtatggagta ggaacagggt atactactac
    31801 agctgtagga gaaacacatt tcgaacgtca atctaacgga tacgttaaat gcttaaaagc
    31861 tggtaagtat tcaattagtg cgcaaatacg tgtgcaattg ggcgggaaat atgcttcatg
    31921 gttatatact gatttatata aagatggaac ttctgttgat aatttagtag cttatggggt
    31981 cgacgccttg caaaatagat ttgcagctag cggaaacatt gtaactgatt tagaagtaaa
    32041 cgatgtacta aatatgcgta ctgaaatcgg cgttgcagat ggaattttac aatttacatc
    32101 ttgccgtact ttagtcttga ctagactatc tgattaatac aatatttata attttgctat
    32161 caacacgctc aaaggagggt gttttttatt ttgcaatgaa aggaggctag ttggttgaaa
    32221 gacgaagcaa tacaagacgt ggtagaacgc ttagtgcgta ttgaaacgaa actggataat
    32281 tacgaattat tacgtgaaaa agcggaaagt gcaaaagata gagcggatca ggcatattct
    32341 attgcgctta ataatgcaga agacatcaaa gagatgaaag ccaataataa atggtcgtgg
    32401 ggttacatga tcggtttagg cattacaatc attggctatt tcttgactaa attgtaaagg
    32461 aggtgagaag aaatggtttt accagataag tattatcaag tcattaaatg gacagtttta
    32521 acagtattgc cagctgcatc tgtattagta gcgacgttag ggaaagcgta tggatggaat
    32581 ggaacagata tgacagtact cactatcaat gcagtagcaa catttttagg tgttatcact
    32641 ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat
    32701 tactgcgatg agcctattaa cggctctttt tttattgcca attaatgggt ttgcctatac
    32761 tatcaacaat gaatttaatt tgggcgcaaa tgaaggtagc tcacaagtag caaataatca
    32821 gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta
    32881 tatgcaacgt tcatggacta gtgcttacac tgcttacatt gtgggtgatg gcggaattgt
    32941 ttaccaagtt ggacaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgccaa
    33001 cagtcctgtg cagattgagt tacaacacac acataataaa gcaacttttg aaaagaacta
    33061 taaagcatac gttgaattgg cgagagattc cgctaagaaa tacggtattc cgcttacatt
    33121 ggacacgcct tataaccaac caggaatcaa atcacattta tgggtaacac aaaatatctg
    33181 gggagatcat actgatccat acggttatct ttctgaaatg ggcgtaagta aagaaaaatt
    33241 agcatatgat ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc
    33301 tgtcattgat ccaacacgag ctggtgcagc taatcctaca ctgacagatg gaacgaatta
    33361 cgcccacatt gatcagtttg gagaaatcga aaatgcaaat ttgcatgtag ctggatggca
    33421 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
    33481 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
    33541 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
    33601 cgtcatgatg cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt
    33661 ccatgacaaa cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga
    33721 gcattacata actatattga caactataaa aatcattcga taaaatagtg atgttatcgc
    33781 atatcttcac tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg
    33841 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
    33901 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
    33961 acattaacgt agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa
    34021 gctaaagctt ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga
    34081 ttaatgaaag gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat
    34141 ttttaaatag aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc
    34201 gcaccccaaa ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc
    34261 attaaaaagc ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg
    34321 tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat atagatatca
    34381 tcatattaca caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata
    34441 aaattcgttc cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc
    34501 aacgaaagta tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc
    34561 agtc
//
LOCUS       DAZUKD010000020        33148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000020 DAZUKD010000000
VERSION     DAZUKD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.020"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG8N9_000803"
     CDS             <1..277
                     /locus_tag="ACG8N9_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896426.1"
                     /translation="MNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSF
                     FVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(411..536)
                     /locus_tag="ACG8N9_000804"
     CDS             complement(411..536)
                     /locus_tag="ACG8N9_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4896427.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(670..1563)
                     /locus_tag="ACG8N9_000805"
     CDS             complement(670..1563)
                     /locus_tag="ACG8N9_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4896428.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1745..2671)
                     /locus_tag="ACG8N9_000806"
     CDS             complement(1745..2671)
                     /locus_tag="ACG8N9_000806"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4896429.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2756..3517)
                     /gene="pflA"
                     /locus_tag="ACG8N9_000807"
     CDS             complement(2756..3517)
                     /gene="pflA"
                     /locus_tag="ACG8N9_000807"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4896430.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3761..5992)
                     /gene="pflB"
                     /locus_tag="ACG8N9_000808"
     CDS             complement(3761..5992)
                     /gene="pflB"
                     /locus_tag="ACG8N9_000808"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4896431.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKSNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6269..8719)
                     /gene="parC"
                     /locus_tag="ACG8N9_000809"
     CDS             complement(6269..8719)
                     /gene="parC"
                     /locus_tag="ACG8N9_000809"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4896432.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8731..10776)
                     /gene="parE"
                     /locus_tag="ACG8N9_000810"
     CDS             complement(8731..10776)
                     /gene="parE"
                     /locus_tag="ACG8N9_000810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4896433.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10942..11376)
                     /locus_tag="ACG8N9_000811"
     CDS             complement(10942..11376)
                     /locus_tag="ACG8N9_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4896434.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11503..12141
                     /gene="plsY"
                     /locus_tag="ACG8N9_000812"
     CDS             11503..12141
                     /gene="plsY"
                     /locus_tag="ACG8N9_000812"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4896435.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12273..13148)
                     /locus_tag="ACG8N9_000813"
     CDS             complement(12273..13148)
                     /locus_tag="ACG8N9_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4896436.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVIRYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13230..14021)
                     /gene="codY"
                     /locus_tag="ACG8N9_000814"
     CDS             complement(13230..14021)
                     /gene="codY"
                     /locus_tag="ACG8N9_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4896437.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14039..15439)
                     /gene="hslU"
                     /locus_tag="ACG8N9_000815"
     CDS             complement(14039..15439)
                     /gene="hslU"
                     /locus_tag="ACG8N9_000815"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4896438.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15453..16001)
                     /gene="hslV"
                     /locus_tag="ACG8N9_000816"
     CDS             complement(15453..16001)
                     /gene="hslV"
                     /locus_tag="ACG8N9_000816"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4896439.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16038..16943)
                     /gene="xerC"
                     /locus_tag="ACG8N9_000817"
     CDS             complement(16038..16943)
                     /gene="xerC"
                     /locus_tag="ACG8N9_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4896440.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17141..18517)
                     /gene="trmFO"
                     /locus_tag="ACG8N9_000818"
     CDS             complement(17141..18517)
                     /gene="trmFO"
                     /locus_tag="ACG8N9_000818"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4896441.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18486..20564)
                     /gene="topA"
                     /locus_tag="ACG8N9_000819"
     CDS             complement(18486..20564)
                     /gene="topA"
                     /locus_tag="ACG8N9_000819"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4896442.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20686..21543)
                     /gene="dprA"
                     /locus_tag="ACG8N9_000820"
     CDS             complement(20686..21543)
                     /gene="dprA"
                     /locus_tag="ACG8N9_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4896443.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21599..22366)
                     /locus_tag="ACG8N9_000821"
     CDS             complement(21599..22366)
                     /locus_tag="ACG8N9_000821"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4896444.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22359..23219)
                     /gene="ylqF"
                     /locus_tag="ACG8N9_000822"
     CDS             complement(22359..23219)
                     /gene="ylqF"
                     /locus_tag="ACG8N9_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4896445.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23480..23878
                     /locus_tag="ACG8N9_000823"
     CDS             23480..23878
                     /locus_tag="ACG8N9_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4896446.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24115..24657
                     /locus_tag="ACG8N9_000824"
     CDS             24115..24657
                     /locus_tag="ACG8N9_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4896447.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(24740..25291)
                     /gene="lepB"
                     /locus_tag="ACG8N9_000825"
     CDS             complement(24740..25291)
                     /gene="lepB"
                     /locus_tag="ACG8N9_000825"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4896448.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25443..25670)
                     /locus_tag="ACG8N9_000826"
     CDS             complement(25443..25670)
                     /locus_tag="ACG8N9_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4896449.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25667..26185)
                     /gene="msrA"
                     /locus_tag="ACG8N9_000827"
     CDS             complement(25667..26185)
                     /gene="msrA"
                     /locus_tag="ACG8N9_000827"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4896450.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26205..26918)
                     /locus_tag="ACG8N9_000828"
     CDS             complement(26205..26918)
                     /locus_tag="ACG8N9_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4896451.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(26921..27817)
                     /locus_tag="ACG8N9_000829"
     CDS             complement(26921..27817)
                     /locus_tag="ACG8N9_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4896452.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(27975..28817)
                     /locus_tag="ACG8N9_000830"
     CDS             complement(27975..28817)
                     /locus_tag="ACG8N9_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4896453.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            28949..29602
                     /locus_tag="ACG8N9_000831"
     CDS             28949..29602
                     /locus_tag="ACG8N9_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4896454.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29666..30193)
                     /locus_tag="ACG8N9_000832"
     CDS             complement(29666..30193)
                     /locus_tag="ACG8N9_000832"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4896455.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30213..31160)
                     /locus_tag="ACG8N9_000833"
     CDS             complement(30213..31160)
                     /locus_tag="ACG8N9_000833"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4896456.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31172..33055)
                     /locus_tag="ACG8N9_000834"
     CDS             complement(31172..33055)
                     /locus_tag="ACG8N9_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4896457.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         8734 a         6976 c         5736 g        11702 t
ORIGIN      
        1 catgaatggt ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt
       61 tagccaattg aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag
      121 cttgttttcc ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt
      181 cgcaggaaat ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag
      241 aaaaaacaaa caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt
      301 ttttagtttt aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga
      361 ctcgccattc gtttcttagt gattctggat tatcaaatgg acttcctaat ctaatcagct
      421 agttcttccc ataaaatgcc atacttgact gaatcataat attgattatt taaaaatcta
      481 acttttctta tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct
      541 ttgtttccag accatgtagt gaatctaata ggtggtagat aggcaaaata ctcaaataat
      601 tcttttaacc ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt
      661 actgctctct taggttttac tcactaaaac tgttccatat tctttgatat aagaaaatgc
      721 atgcggtata aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccatttc
      781 ctccattgtc aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac
      841 aataggtaat tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga
      901 ctgatggaaa atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat
      961 aacttctatt tgatcaaaaa agtacgattc atttacgtct ccaataagtt cggctaattc
     1021 tttgaatgcc ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt
     1081 aagcgggaag tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt
     1141 tgtcctcagc aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg
     1201 ttcatcttcg aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac
     1261 agagagtctc ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta
     1321 atctttagga tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa
     1381 tcctgcatcg atcaataaat caagaaaatg ggaaaattta ttttgcgaaa attctttttt
     1441 caattctctt agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga
     1501 aaaaagcgga tcatcggtca gtttttgata aaagttttcc ttattttttg aataatagtt
     1561 catatatgtt ctcttttcta tccgttttat tttacttaaa aggcttttac gtaaaaagga
     1621 ggaagaacaa gagtcataat tgactgttat tctcctccat ttcttcggcc tttagtatgt
     1681 tctatgtttc tttttatatc aatcgcttat ccattcattt tgaatgcttc atactaccat
     1741 ttgattagct aaatacagcc gtcaattgag gtacgacttg ttttttacgt gaaacgacac
     1801 ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa agcttcttct actttttctt
     1861 ttcctttacc agcaacaagt aattctgaat cactgtctaa gatgtttgtg acaactaata
     1921 cgaacagatc gtagccattt gctttcattt catcttccat tgctgcttct agctctgctt
     1981 gtcgatccat cacttcatta aggtccactg tattgacttg agcgatacga acgttggaac
     2041 tgcccattgg gaaacttttt gcatccaaat caagaagaac cgcagctgat ttatcactta
     2101 gatttgttcc tgctttcaac atttcaagtc cataagtgtt tagatcgact tcagcgattg
     2161 ctgcaagttc ttttgcagct gcttcatctt caggcgtaca tgttggtgat ttgaacaaca
     2221 atgtatctga aatgatagct gagagcatga ttcctgcgat ttctttagga attgttactt
     2281 ggttttcttt atatagtttt aaaataatcg tacttgtaca tccaactggt tctgcacgat
     2341 agtacaatgg attagctgtt tcaaagtttg caatacgatg gtggtctact acagctaaaa
     2401 tatttacttc gctgatatct gaaatacttt gttggaattc attatgatcc acaagcatga
     2461 catctgcatt ttctgcttta gctgactcta ctactcgtgg tgcagatagc tgaaagtggt
     2521 ctaatgcgta ttgtgtttct tcattaggtg tacctagagc aactgcttct gtttctttac
     2581 ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc gattgcatct gtatctggat
     2641 tttggtgacc aaaaactaaa atttttgaca tgtcattcgc tcctttgtga tttctcctat
     2701 ttaataatag caaaaaaacg gaaagaagca atacttatct tctttccgat gcgacctatc
     2761 gcgttaaata ttttgtatac tcttctgtat gaagcaatcg tttagcattt tcgacacgtt
     2821 cttttgtagg tggttcgatc ccttctaatg gatatttcaa accaagtgtt tcccacttgt
     2881 attttcccat cgtatgataa ggaagaatct ctactctgtc tacatttgac aaggtttgaa
     2941 taaagttgtc tagtcggatc aaatattcat cataatcact tcgctcagga actagtacat
     3001 ggcgaatcca aaccggtttg ttgatttttg acaaatattg agccatttct aaaatatttt
     3061 cattgtccca atgcgttagt tttttatgct cttcattatc gatgtgtttg atgtcaaata
     3121 acagtaaatc tgtatacttc atcaattctt gaaaacgtga aaagaaaggt tcttcatacg
     3181 taaaaggttt tccgcatgta tccaatgtag tatgcatctt ccgctctttt gcttttttaa
     3241 atagttcgat caaaaaatcg atctgcaata gaggttctcc accactaaca gtaataccac
     3301 ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc taaaagttca tctgctgtgt
     3361 attctttccc tccgccaata ttccaagtat ctggattgtg acaaaattgg caacgcatgc
     3421 ggcatccttg cataaacacg acaaagcgca gtccaggtcc gtctactgaa ccgaaagttt
     3481 cgatagaatg aacatagccg attgtttttt cttccattta agttcacatc ttttctttga
     3541 tttcatttta agtacaagtt agctgtcgaa gcgaagcttt ctgaacaaaa tttttcgcaa
     3601 ttgccgcttc gttatgtgta aaatttacag ggacggaaca tacaagtcct gcccctgtaa
     3661 atcgtttctt tcaaataagc atactgatta ttcgattttg atgcgatggc tattcaacgt
     3721 cctttcgacg ctaagtcgaa aaactttttt cacgaacttt ttacattctg tcgtgtgaag
     3781 ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc acggaattta actgcataac
     3841 cagatacccg gatcgtcaag ttcggatatt tcattggatt ttccattgca tcacgcaata
     3901 aatcattact gaatacattg acattcaaat gataagctcc ttttgagaag tatccatcta
     3961 agacattacg caagttttcg attcttgtat cttgttcttt tccaagacct gaaggattga
     4021 ttgtttgggt atttgaaatt ccatccaatg cacagccgta attcatttta gctgttgagt
     4081 tcaggctagc taataaccca ctttgttccg cattgtaact tggatttgct cctggcgcaa
     4141 gtggttttcc agcttcacgg ccatcaggag tatttcctgt atttttaccg taaacaacat
     4201 ttgaagtaat cgtcaataat gaagttgttg gttttgagtt gcggtaagtt ttatgacgtt
     4261 tgatttgtcc catgaagtat tccaagatcc atgaaactaa ttcatctgca cggtcatcat
     4321 catttccgta tttagggaag tcgccatcca cttcaaaatc aactgctatt cctttttcat
     4381 tacggattgc ttttactttt gcatatttga tcgctgacaa gctgtctgcc gcatgagaga
     4441 tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga atccatcaaa gccagctgag
     4501 ctgcttcata agaatattta tcatgcatat aatgaatgat gttcaacgta ttgacatata
     4561 gttctgctaa ccaatccatc atattcatga agttatgttt cacttcttca aagtctagta
     4621 tttctgatgt gattggacga tattctggtc caacttgggc gccagtcact tcatcgactc
     4681 cgccattgat ggcatataat agtgttttag ccaagttagc acgagcgcca aagaattgca
     4741 tgtcttttcc gacaactgtt cctgatacac agcaagcaat cgcacaatca tcagaacccc
     4801 attgttcacg aagctgatta tcattttcga attgaatcga agaactttcg atagcgattc
     4861 tgctagcata tgttctgaat ccttctggaa ggtgttcaga atacaaaaca gtcaagtttg
     4921 gttctggtga aggtcccata tttgttaatg tatgaagcag acggaaatca ttctttgtaa
     4981 ctaatgaacg accatctaat cccatacctg caatggataa agtagcccag atagggaatc
     5041 ctgagaataa ttggttatat tcaggtgtac gcgcgaattt gaccatacgt aatttcatta
     5101 ctaagtggtc gatcaattct tgtgcttctt tttctgtgat tactccatta tccaaatcac
     5161 gttggatata gatatcaagg aatgctgaga tacgtccgat tgacatcgca gcaccatttt
     5221 gtgatttgat tgctgctaaa tatccgaagt atagccattg gacagcttct tttgcatttg
     5281 cagctggttt agaaatatca tagccatatt tcgcagccat ttctttcatt tgcgctaatg
     5341 cacgatattg ttcagtgatt tcttcacgta aacgaatcac ttcttcactc attgtatgat
     5401 aaccagtcat atcatgatct tttttctttt gttccatcaa atagtcgatt ccatacaatg
     5461 ccacacgacg atagtcaccg atgatacgtc cgcgaccgta agcgtctgga agccctgtaa
     5521 tgattttgtt tttacgagca gcacgcattt cagcagtata agcgtcaaat acaccttgat
     5581 tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc atcgtttggt gtatagccat
     5641 tgctttttag cgcattgtta gccatattga ttccgccgaa cggcatgaat gcttgtttca
     5701 aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc tttatccaaa tagcctgcat
     5761 cgtgtgaagt aatagttgat acgatttctg tgtccatatc atagacaccg ttacgctcga
     5821 attggatatc atttgacttt tgcaattcat cccataattt ttctgttgct tctgttgggc
     5881 cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt attttgaata aagtctctag
     5941 tgtctacgcc ttctttccat ttggtacctt taaatccatt ccattgctcc attgtatgga
     6001 cctcctaaat ttctgtttta tgtaacagtt tttacgtgat tagtataaca cagcacccta
     6061 tctttgcaag ggttttcttg ttttttattt aacaattttt atgttttatt tacatatatt
     6121 taaatcaacg ttgacatacc aacatttaag cattgtgaac actttcatct ttttttgcaa
     6181 acgtataagg atttattaaa aatgaatgaa aaagagacgc cacctgtatt aatacagatg
     6241 gcgtctcttc attaaaatac tacaacagtt acgactcgat gaaaatctca ggagcatcga
     6301 taacttcgat gacttctcca ccttttttct catccatcgc aaatgatcca ttcgatgttc
     6361 gatcacctat agggaaatca gttgggtcaa tcgttaactg ctgccctttt tgattcatca
     6421 aggttatttc ttttgatctt ccttccgaca tatagataat acggtgcgga ttttttttca
     6481 gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt ttggctgatt tcttgagcaa
     6541 gcatgcgttt aacagctcct cgctgagtaa cgattacgat cggcgtatca ccttctgcta
     6601 acacaagcag cccgttgata atcctatctc catctttcaa attcatagac ttcactccag
     6661 ctgcctttgc tccaacaacg ggaacatctg ttaaaggata gcgcaaaccc attccttgat
     6721 gcgttactaa gaaaacatct aagcctggct gatcttcttt cagatacaca gcaatcagtt
     6781 catcttcttc cgcctttagc ttcatacagc tgagcggacg gctcttatag gtgcgccatg
     6841 gttcgaactc tgtcattcgt gtttgcttga tcaatccatt cttgctgatg aatacaaatg
     6901 ttttctctgc ttgaattttt tgatatggga aaacagctaa aatcgattcg tctacagaca
     6961 gattggagat tgtttgagaa atgtgctcac cagcatcttt ccatttcaaa tcgggtagtt
     7021 catggacagg gcggtagatc acatttgctt tatttgtgat cagaagaaca tggtccaatg
     7081 tactcaattc acctgagtat agcaagtagt cgccttctcg catcccgatt tcttctggtt
     7141 tggaagcact ataagaacga aggcttgttc gcttcacata gccttctcgc gtcacactga
     7201 caattacgtc ttcttgtgcc actaagactt gcgtatcaat tttgatttct tcgatttcgt
     7261 cttcgattgt cgttagacga gcggaagaat aatttttttt cacttctcgc aattcttttt
     7321 tcataacaga gaaaagctct ttgtcatttg aaaggatttt attcaactct gtgatttgcg
     7381 caatcaagct ttcggattcc ttttgaagct ctgtgatatc cgtgttcgtc aaacggtata
     7441 attgcaatgt gacaattgct tctgcttgtt cttctgtaaa ttgaaagacg tcgactaaat
     7501 tctttttggc gtcttttttg tctttacttt cacgaatcgt tgcgattact tcatccagaa
     7561 tcgataaagc tttcatcaat ccttctacga tatgctgtct tttttgagct ttggctaaat
     7621 cgaattgact acgttttgtt atgacttgct ttcgatgctc gatataactt cttaaaatgt
     7681 ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc gaccatattg aaattgtaat
     7741 tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat tccttgagca ttggcgtctt
     7801 ttttcaattc gacgacaatt tgcagacctg ttctatcact ttcatctcgt acttcagcta
     7861 ttccatcgat tttcttattc aaacggatct catccatttt tttcaccaag gtcgctttat
     7921 ttacttcata tgggatttct gtgatcacga tttgctgttt attacccttc attgcctcta
     7981 tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt ttcataagct tttttgattt
     8041 catcttttcc ttgcaagatc cctcctgttg gaaagtcagg tccaggaata tattccatca
     8101 atttttcaag gctcgcttgt ggatgatcga tcatatagat tgtgccatcg atcacttcag
     8161 ctaagttatg agtcgggatt tctgttgcat atccagcaga tatacctgtc gatccattga
     8221 cgagaaggtt tgggtatcga gctggaagga ctgttggttc tttttctgta tcatcaaaat
     8281 tccacacgag atcgactgtt tctttctcga tatctgctag catctctcca cttaacttgg
     8341 ataatctggc ttcagtataa cgcatcgcag caggtggatc accgtccata ctcccgttat
     8401 tcccgtgcat ttcgatcagt acttcccgca acttccagtc ttggctcatg cggaccatcg
     8461 cttcataaat actgcggtca ccatgagggt gataattccc catgatattt ccgacagatt
     8521 ttgctgattt acggaatcct ttgtcatacg tattgccatc tttgttcatg gcaaataata
     8581 tccggcgttg cactggcttc aatccatcac ggatatcagg cagtgcacgt tcttggataa
     8641 tatatttgga ataacgtccg aagcggtcac ccatcacttc ttctaaagtt aattcttgta
     8701 cttcttgtcg attttccatc aggtgacctc ctattctact tcaaataact gattgttttc
     8761 atttttatct gctcgttctt catctaataa atcattagaa actgaaggag agatttcagt
     8821 atcctctttt ttatctaaga tactgccatc ttcttcgagc gtaaattgca cgtgattttc
     8881 gatccatttt cttcgaggtt ctactttgtc acccatcagt gtggttactc gacgttcagc
     8941 ttgcgcagca tcatcgatac ggacgcggat caacgtacgg gaagtcggat ccatcgttgt
     9001 ttcccacaat tgttccgcat tcatctctcc aagtccttta tatcgctgca gcatatatcc
     9061 tttgccgact tttttaatca ttgctgccaa ttcgtcatct gtccacgcat attcaatgac
     9121 ttgcttcttt ccttgtcctt tggatacttt atataaaggc ggtaacgcga tatatacttt
     9181 cccagcttca atcagcggtt tcatataacg ataaaagaaa gtaagcagta atacttgaat
     9241 atgggcgccg tctgtatccg catcggtcat gatgattact ttgtcgtaat tacagtcttc
     9301 gatagaaaac tcaggaccga ctcctgcacc aatcgtatag atcatcgtat tgatttcttc
     9361 atttttcaag atatcttgca ttttagcttt ttcggtattg atgacttttc ctcgtaaagg
     9421 tagaattgct tggaactttc tgtctcttcc ttgtttagca gatccgccag cagaatcccc
     9481 ttcgactaaa tacaattcat ttttcttagg gtttcgggat tgtgcgggtg tcaattttcc
     9541 tgaaagaaga gattcgcctt ttttacgctt cttcccattt cggctttctt ctctggcttt
     9601 acgtgctgct tctctcgctt ctctggcttt gattgcttta cggaccaaca tctggctcat
     9661 ttcgctattt tcctgcaaat aaaagcccat ttgttcactg atgacgttat ctactacggt
     9721 acgagcaact ggcgtaccaa gtttttcttt tgtttgccct tcaaattgca gaagattttc
     9781 aggtacacgg atagaaagta cagcagctaa cccttctctg aaatcactac cttccagatt
     9841 cttgtcccgt tctttcagca aaccaacttt tctcgcatat tcattataag atttcgtcat
     9901 cgccgctttc ataccagctt catgggttcc cccatctttt gtccgaacgt tattgacaaa
     9961 agataaaaca ttttctgaat aaccgtcatt gtattgataa gcgacctcta cttcgatacc
    10021 ttctttttct cctgaaaaat aaacaactgg tgtcaatgta tctttttctt cattcagata
    10081 atcaacaaat tctttgattc cttcttcgta atgaaaaatt tccttgactg gttcttctcc
    10141 acgaagatca gaaagagaga ttttaacgcc ttttagaaga aaggcagatt ctcttaaccg
    10201 ttcggctaaa atttcatatg aaaatttcgt tgtactgaat attgtatcat ctggtaaaaa
    10261 atggacagtc gtcccgtttg ctttttttgt ttttcctatc tttttcaaag taccgtcagg
    10321 tttaccgccg tttttaaatt tctgctgata ctctacgcca tctctaacga tcgttacaat
    10381 tagccatttc gataaggcat ttaccacact ggctcctaca ccgtgcagtc caccagaagt
    10441 tttgtaccct ccttgaccaa actttccgcc agcatgcagc acagtaaaaa tcacttcaac
    10501 tgttggtatt ccggaagcat gcattcctac aggcatccca cgtccgctat ctgcaacagt
    10561 tatactattg tcttcatgga tggtcacatc aatctcactg ccataacctg ataatgcttc
    10621 gtcaactgca ttatcgacga tttcatagac taaatgatgc aagcctcggc tgtcagtgga
    10681 tccaatgtac atccctggtc tttttctaac tgcctctaat ccttccaata cttggatgga
    10741 cgagtcattg tattcgttgt ttactttttt tgccaaggca aacgctcctt caataatact
    10801 ttgcggcaaa accgcaccac gattatcata ctagaaaagg tcgtgaaaaa aaagagggta
    10861 gacgagaaat caattttaat tatctcctaa ccgctttttc gacattcccc tctatcgatc
    10921 atctcttctt tgtggtcatt tttatttttg gttttctttc aacttaccaa gttcaatctt
    10981 aatacaacga tccattacga tatctgtacg tcctgctttt cttagcatct cagctgcttc
    11041 tttgttttca atccctaatt gtgcccagaa cactttagca tcagttttca aaaaatcacc
    11101 tgctatttca ggtaaaaatt cactccgtct aaatacatct acgatatcga ttggatcatc
    11161 gatttctgtc aaattcgcat agactttctc gcctagaatt tcttcaccag ccaataatgg
    11221 atttactggt atgattttat atccattgtc ttgcaatacc tgtgcaacca tgtaacttgt
    11281 tcgttctggt ttattgctca agcctacgac ggcaatattt tttgcttctt ccaaatagtt
    11341 gaaaatcgtt tcttgtgttg gattttttac agacatttct gccacccctt gcttttcttt
    11401 ttattccatc tattatactt gtatctttca ttttttcaat tgttcctgtt acctattcat
    11461 gctaaaataa aggatagttt tcaacttgaa agagggtaaa aaatgaaatt gatcttatta
    11521 cttattttag catatctttt aggttctatt ccttccggtg tctggattgg caaattgttt
    11581 ttcaaaaaag atatccgaca acacgggagc ggtaatactg gaacgaccaa tacatttcgt
    11641 gtattaggca aaacagccgg gattgttgtc ctgcttatgg atattttaaa aggtacattg
    11701 gctacttgtt tgccgatgat cttccacctt tctgggatta accctctttg gtttggggta
    11761 tgcgccattc ttggccatac atttccgatt tttgctgggt tcaaaggagg aaaagctgtt
    11821 gcgacaagtg caggaatgct tttaggattt aatcctgtat tttttgttta ttcttgtatc
    11881 atctttatcg ttagtttatt ttgtacaagt atggtgagtt tgagcagtat gatcagtgca
    11941 gtattgatca ctttatctac gatcgtcctt ccttatgtag ctcctgtgat tttggctaag
    12001 cccaattggt tactgacgat cattgccttt ttagtttctt cctttatctt ttatcggcat
    12061 aaagacaata tcaagcgtat cagaaaccat actgaaagtc gaatcccgtt tggtttgaat
    12121 agcagcaaga aaaaatcctg atactttttc attccaaaga gataaaacgg gagtgtgaca
    12181 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
    12241 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
    12301 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
    12361 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
    12421 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
    12481 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
    12541 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
    12601 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
    12661 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
    12721 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
    12781 gtgatgccgt ctcctcctaa ttcatacctg atcactaatt caaaatcaaa tggatacttt
    12841 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
    12901 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
    12961 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
    13021 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
    13081 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
    13141 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
    13201 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
    13261 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
    13321 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
    13381 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
    13441 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
    13501 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
    13561 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
    13621 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
    13681 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
    13741 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
    13801 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
    13861 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
    13921 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
    13981 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
    14041 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
    14101 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
    14161 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
    14221 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
    14281 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
    14341 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
    14401 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
    14461 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
    14521 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
    14581 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
    14641 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
    14701 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
    14761 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
    14821 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
    14881 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
    14941 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
    15001 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
    15061 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
    15121 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
    15181 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
    15241 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
    15301 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
    15361 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
    15421 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
    15481 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
    15541 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
    15601 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
    15661 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
    15721 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
    15781 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
    15841 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
    15901 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
    15961 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
    16021 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
    16081 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
    16141 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
    16201 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
    16261 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
    16321 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
    16381 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
    16441 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
    16501 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
    16561 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
    16621 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
    16681 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
    16741 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
    16801 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
    16861 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
    16921 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
    16981 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
    17041 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
    17101 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
    17161 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
    17221 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
    17281 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
    17341 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
    17401 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
    17461 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
    17521 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
    17581 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
    17641 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
    17701 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
    17761 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
    17821 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
    17881 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
    17941 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
    18001 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
    18061 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
    18121 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
    18181 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
    18241 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
    18301 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
    18361 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
    18421 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
    18481 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
    18541 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
    18601 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
    18661 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
    18721 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
    18781 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
    18841 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
    18901 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
    18961 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
    19021 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
    19081 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
    19141 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
    19201 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
    19261 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
    19321 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
    19381 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
    19441 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
    19501 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
    19561 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
    19621 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
    19681 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
    19741 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
    19801 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
    19861 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
    19921 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
    19981 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
    20041 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
    20101 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
    20161 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
    20221 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
    20281 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
    20341 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
    20401 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
    20461 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
    20521 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
    20581 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
    20641 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
    20701 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
    20761 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
    20821 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
    20881 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
    20941 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
    21001 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
    21061 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
    21121 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
    21181 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
    21241 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
    21301 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
    21361 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
    21421 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
    21481 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
    21541 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
    21601 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
    21661 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
    21721 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
    21781 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
    21841 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
    21901 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
    21961 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
    22021 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
    22081 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
    22141 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    22201 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    22261 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    22321 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    22381 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    22441 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    22501 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    22561 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    22621 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    22681 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    22741 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    22801 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    22861 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    22921 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    22981 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    23041 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    23101 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    23161 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    23221 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt ttttcttttt
    23281 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    23341 ccagtcctca ttctatcaca tcttcacttt tttgatgatg taaattttta ttttggtttc
    23401 tatatatgaa tctaaatttt tgacaacgtt tcctaaataa gagatgatag acgaacaaaa
    23461 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    23521 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    23581 tgaagaagct ggttcaatgg tatgggatga cactttttgg gtaatgcttc tctctcatga
    23641 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    23701 tttgatttcg tttagtgttg aaaacgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    23761 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    23821 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    23881 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    23941 tctccgcctc tatttttttc attcgtacaa tctctttttt acagtttata aaagcagtct
    24001 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    24061 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
    24121 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
    24181 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
    24241 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
    24301 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
    24361 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
    24421 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
    24481 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
    24541 gtgatttttc taggtagagt catcaggaat atgatccaaa tccctattca aataatggtt
    24601 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
    24661 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
    24721 ttgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
    24781 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
    24841 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
    24901 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
    24961 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
    25021 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
    25081 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
    25141 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
    25201 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
    25261 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct cgttttttca
    25321 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
    25381 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
    25441 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
    25501 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
    25561 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
    25621 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
    25681 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
    25741 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
    25801 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
    25861 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
    25921 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
    25981 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    26041 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    26101 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaaacagc
    26161 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    26221 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    26281 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    26341 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    26401 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    26461 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    26521 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    26581 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    26641 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    26701 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    26761 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    26821 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    26881 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    26941 ctagccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    27001 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    27061 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    27121 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    27181 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    27241 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    27301 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    27361 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    27421 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    27481 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    27541 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    27601 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    27661 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    27721 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    27781 acaaaacgag gatcagatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    27841 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    27901 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    27961 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    28021 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    28081 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    28141 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    28201 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    28261 cgtatgttca aaacactaga cagcaaacca gttgttcgac tgattcttcc accttttacc
    28321 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    28381 tcttcaagga tttcctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    28441 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    28501 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    28561 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagataaa ttctccaatc
    28621 ggtggttggc ttgttttggg cagatttttt gcttttgcca tcatttccat aaatctttcg
    28681 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    28741 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    28801 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    28861 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    28921 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    28981 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    29041 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    29101 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    29161 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    29221 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    29281 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    29341 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    29401 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg gtgttatttt
    29461 gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct tgaattctat
    29521 ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt
    29581 ctctgtttta ttctttactt aataaaaaaa aataaagcta tggatgggac agctctaaat
    29641 aacagctagt ctatccatag cttttttata tcgtcaattc gcctgtcccg ctttttacag
    29701 gattctcatt acgctgatac gtttcaaatt gatgcggata acgatttttt tcatctattt
    29761 ctccttgact agtattgatc agtgaccaat cagaccagtc aacaggtgga aaataagcat
    29821 ctccatcgaa tacatgatgg atgactgtac ggtataatat cgtacaaaat ggcagaaatt
    29881 cttgataaac agctgaccct ccagcgatga aaaagatacc gtctgtttct ttttccagag
    29941 caagcacgtc ttccacacta tgcatgacat gcacgccttc tgctttatag gaagaatctc
    30001 tcgtcaaaac aatcgtctga cgattcggca acggacggct acccatccct tcgaaagttt
    30061 ttcgtcccat cactagtgta tttgcttctg tcatttgttt gaagaatttc agatcatttg
    30121 gcaaacgcca aggaagacgg tcttcttttc caatcaatcc gttttcatcc tgtgcccaaa
    30181 ttgcagctaa cattttctca tcctttctga ttctaaacag cgataggagc tttgatcgcc
    30241 ggatgtgggt catatccttc taattggata tcttccatct caaaatcaaa tactgactgc
    30301 ttttcctgat tcaaaactaa agtcggaaat gcgcgtattt ctcggctcaa ttgttctttc
    30361 atttgttcga tatgattgat ataaagatga gcgtctccta atgtgtgaac aaattcgcca
    30421 accgctaaac ctgtttcatg tgcaattagg tgagtcagca acgcataact tgcaatgttg
    30481 aacggaacgc ctagaaacac atccccactg cgttgataca attggcagct tagccgtccc
    30541 tcttgaacat aaaattgaaa catcgtatga caaggaggca aagccatgct aggaacatct
    30601 tccggattcc atgcagaaac gatcaatctt ctagaatcag gagtcttttt gattgcttcg
    30661 atcacttctt tgatttgatc gataaagcca ccgtcttttg tttcccagtg acgccattga
    30721 tagccgtaga tgtgtccaag atcgccgtat gtttcagcaa aggaatcatc ggttaggatc
    30781 ttctcgcaaa aaagcttgct ctgcttttta tattcttcat tgaattcagg atctgtcaaa
    30841 catctatggc caaaatcagt catatctggt ccattatagt ctggactttt gatgtagcgt
    30901 tcaaatgccc attcatccca aatatggttt ttattttgta atagatagcg gatattcgta
    30961 tctcctttta aaaaccaaag caattcgctt ttgatcaatc caaaaggtac tcgtttcgtt
    31021 gttaacaaag gaaacccttt ttgcagatca aaccgcattt ggtatccgaa taagcttaat
    31081 gtgcctgtgc ctgttctgtc ttctttacga tgcccttctt ccaacaattt tcttcctaag
    31141 tcaagatacg cttgttccat cctttttcct cctaatcttc tacccattca ctcaagtatt
    31201 cccaccgtgc cattttttct tcaagttgtg cttctgtttc agatacgtcg ttttgcaatt
    31261 cttgtaatcg agtgaaatta tctccttgat gattcatctc ttcttggagc aatgagattt
    31321 tttcttcaag ttctgcaatc tcatcttcaa tcgtttccca ttctttttgt tccatgtaag
    31381 ataatttctt cttttcagca ggttccttgc tctgtatttt tggtttcgct ttttcagcag
    31441 gctgctgggt ctctttttgc ttcagtaaat aactggacat gtttccgtaa taaagttctt
    31501 gttttccgtt tccttggaat actaataact tatctgctac cttgtccaag aaataacggt
    31561 catgggaaac agtgatcaca gcacctggaa aacttctaaa ataatcttcc aaaatcgtca
    31621 atgttgctat gtccaaatca ttggtcggtt catccaataa taaaacatta ggttgctgga
    31681 tcagcagttt caacaaaaat aaccggcgtt tttctccgcc tgaaagtttg cctatcagtg
    31741 tcccatgcat aaatcttgga aataaaaatc gttcaagtaa ttctgctact ccgatttgac
    31801 tgccatctgc tgtttttgct tcttcagcag cttcttgtaa gtaagcaatc atccgtttat
    31861 ctggtgccat ttcttcattt tcttgtgtat aatacgcaag atgaaccgtt tcaccgatag
    31921 acattgtacc gctatccagg ggaattctgc cagctagaat attaagcaat gtcgattttc
    31981 ccgcaccatt tttaccagta atacctagtc tttcccgaga ttgaatcagt aaatctaaat
    32041 gttctagcag tgtttggtca tttatacggt aactgccatc tttgatttcc agtacttttt
    32101 tccctaaacg ttgtgtcgct aggttcagct ctaaatcatc ttcttggttt acttggtaca
    32161 agttctcctt tagatcttcg aaacggttga tccgtgcttg ctgcttggtg ccacgtgcct
    32221 gtacgccagc cctcatccaa gctaattcct gtttataaag ccgcttgcgc ttttcttctt
    32281 gttctacttc tacacgatca cgttctgctt tttctaagac gtaagcttca taatttcctt
    32341 tatactcgta taattttcca aatgaaagtt cgaagattcg gtttgctact ctatctagaa
    32401 aataacgatc gtgggtaacc atcaatagtg cgccacggta ttgtttcaaa tagttttcca
    32461 accactcgat cgcttcataa tctaaatggt ttgttggttc gtctagtaat agtaaatcgg
    32521 gttcatcaat caacacttgc gccagactaa ctcgtttttt ctgaccacct gataaagtac
    32581 tgattttttt atctaattca ctgattccta atttttgcaa aataatcttt gcgttggtat
    32641 ctgtagtcca agcatcttct ttattcattt gttcttctgc tttagcataa cgtttttgca
    32701 catcagggtc ttctccgttt tcccctagtt cgatcaatgc ttcctcatat gctcgaacag
    32761 tttggatcaa ggggctgttt ccttgaaaga ctgcttggag aacagttaat tcatcggaaa
    32821 attcagctgc ttgagaaaga taaccaatac ggtaatcagt tggataaaac actgtttgcc
    32881 gatcaccgtc gccgctatcg ataccggcaa taatatttaa gagacttgtt ttacccgtcc
    32941 cgttcgtccc aatcaacccg atacggtctt tttcgtggac caaaaaagaa atctggtcaa
    33001 acaaatcttt ttcaccataa gtttttttga ggtcagttac ttttaattct ttcaacatgt
    33061 tccctcctta tcttggatat tgtatcataa aaatagtgaa cttcaggaaa atttttttgg
    33121 tatttgttct ttttgtgttc tcaaaaaa
//
LOCUS       DAZUKD010000021        32048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000021 DAZUKD010000000
VERSION     DAZUKD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.021"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8N9_000835"
     CDS             6..245
                     /locus_tag="ACG8N9_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896458.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8N9_000836"
     CDS             312..521
                     /locus_tag="ACG8N9_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896459.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8N9_000837"
     CDS             581..1252
                     /locus_tag="ACG8N9_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4896460.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8N9_000838"
     CDS             1305..3281
                     /locus_tag="ACG8N9_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4896461.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG8N9_000839"
     CDS             3294..4049
                     /locus_tag="ACG8N9_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4896462.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG8N9_000840"
     CDS             4046..4870
                     /locus_tag="ACG8N9_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4896463.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG8N9_000841"
     CDS             4880..5128
                     /locus_tag="ACG8N9_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896464.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG8N9_000842"
     CDS             5171..7270
                     /locus_tag="ACG8N9_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS4896465.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG8N9_000843"
     CDS             complement(7308..9365)
                     /locus_tag="ACG8N9_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFS4896466.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG8N9_000844"
     CDS             complement(9479..9691)
                     /locus_tag="ACG8N9_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896467.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG8N9_000845"
     CDS             9878..10345
                     /locus_tag="ACG8N9_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896468.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG8N9_000846"
     CDS             10342..10584
                     /locus_tag="ACG8N9_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS4896469.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG8N9_000847"
     CDS             10602..10904
                     /locus_tag="ACG8N9_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896470.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG8N9_000848"
     CDS             10976..13030
                     /locus_tag="ACG8N9_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896471.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG8N9_000849"
     CDS             13030..15642
                     /locus_tag="ACG8N9_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4896472.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG8N9_000850"
     CDS             15639..18170
                     /locus_tag="ACG8N9_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4896473.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG8N9_000851"
     CDS             18222..18554
                     /locus_tag="ACG8N9_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896474.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG8N9_000852"
     CDS             18555..19148
                     /locus_tag="ACG8N9_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896475.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG8N9_000853"
     CDS             19311..21005
                     /locus_tag="ACG8N9_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS4896476.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8N9_000854"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8N9_000854"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4896477.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG8N9_000855"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG8N9_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG8N9_000856"
     CDS             24078..25283
                     /locus_tag="ACG8N9_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4896478.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMS
                     IINTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG8N9_000857"
     CDS             25299..25943
                     /locus_tag="ACG8N9_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896479.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG8N9_000858"
     CDS             25954..26760
                     /locus_tag="ACG8N9_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896480.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27011
                     /locus_tag="ACG8N9_000859"
                     /pseudo
     CDS             26782..27011
                     /locus_tag="ACG8N9_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27557..27832
                     /locus_tag="ACG8N9_000860"
     CDS             27557..27832
                     /locus_tag="ACG8N9_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896481.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27874..28296
                     /locus_tag="ACG8N9_000861"
     CDS             27874..28296
                     /locus_tag="ACG8N9_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896482.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28315..29907
                     /gene="mobP2"
                     /locus_tag="ACG8N9_000862"
     CDS             28315..29907
                     /gene="mobP2"
                     /locus_tag="ACG8N9_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS4896483.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29919..30254
                     /locus_tag="ACG8N9_000863"
     CDS             29919..30254
                     /locus_tag="ACG8N9_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896484.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30401..30595
                     /locus_tag="ACG8N9_000864"
     CDS             30401..30595
                     /locus_tag="ACG8N9_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896485.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30627..>32048
                     /locus_tag="ACG8N9_000865"
     CDS             30627..>32048
                     /locus_tag="ACG8N9_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4896486.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKK"
BASE COUNT        11834 a         4966 c         6548 g         8700 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 cttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattactga agacagtgcc tattataaaa
    24721 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
    24781 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
    24841 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
    24901 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa
    26461 gacgaagatt atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcaa
    26881 gaagtgaatt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
    26941 ctttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg
    27001 ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa
    27061 tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct
    27121 aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa
    27181 ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat
    27241 atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca
    27301 caaataacct taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt
    27361 gttaggtagg ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat
    27421 ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg
    27481 tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta cacatatccc tacacataat
    27541 aaatttggag gaaaatatgg acttattaat tagaaatatt gaagaaaagt atattaataa
    27601 aattgacaaa cgttgtgaag aacttacggt caagactggc aaaaaatgga gtcgaaatca
    27661 atacctaaaa gttttgatcg aaaatgattt tgatcacgct cttctgaatt ataaaaaaga
    27721 ccaatttgat cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaacttt
    27781 agaagaatat attgcaacaa ataatcaatt aattggtttg ttaattgaat aataaaaata
    27841 gtattgtgaa tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta
    27901 tatctttacg atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata
    27961 tctaattata gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa
    28021 aaaataaatc caattcaaat taaattgaat gcaatgggaa aagaaatttc tattttaacc
    28081 gaaatggttt cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca
    28141 aattcaactg tttatcaaga agcagaagaa cgtgtggaag gaaaaattaa atccaatcaa
    28201 accaaaaaat attcttctaa gttaaagaaa ccagtgaatg aatctgttca taccagagta
    28261 gatccatttg atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat
    28321 aaatcgcctg cagttattct tacttgtcaa tttaccttgc ccaataaaaa atctttttca
    28381 acatacattg attatatgac aagagaaaaa gcactggaag aaatggaacg acgtacgccc
    28441 gaagaagaac aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa
    28501 ggagaaactt tttcggaaag taagacggat aaagaattca gtgaaacgtc gaatgaagct
    28561 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa
    28621 tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagg
    28681 gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa aatatcaaac agagccacat
    28741 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
    28801 agtgatctga aggagatcaa acaaatcttc caaagaggac aacaaaaagg ctcaatcatt
    28861 tatcaagatg ttatctctca cgacaattcg tatcttgaaa aaatgggctt gtacgatccc
    28921 aaaacagata ccttagatga agaaggtttg aaagcagctg gaagaaaaat gatggagaca
    28981 ttattcgaga aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac
    29041 actaagcata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccgtat
    29101 actgagattg aaaatggtgt gagttatatc gtaccgaagg ggaagagaag acaagcaaca
    29161 ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac gtttctctta tgaaaaggat
    29221 cgaatgatcg gtatggagaa acgaaattta ttgacacgaa aaagtgattt gcgtaacacc
    29281 ttaacaaaag tagtaaaaga accagcacgt tacgatcgac acgccatgaa cttattaaat
    29341 gaagtatata aaaccctacc tgaaaaacgt tcggaatgga attatggaga tgaaaatcga
    29401 accaagcttt caccatttac aaggggaaaa cttgatgagt taaccaaata tgttttaaca
    29461 aaagaacctg gatatcaaga atatatacag atttcaaaag cattgaaaga agaagccaaa
    29521 agcctttatg gggaaagcaa aagagaaaca aagaatgcag aaaaaaacgc cctctttgat
    29581 ttaaaaaagc gcacaggaaa tgcgatttta acagaactga agagacagga tagtcaaata
    29641 aaaccattaa tagaaagtgt caatcagatg caatctattt acaatgaacg atcctttcga
    29701 acactcaaac agttgccaac aaatgaatac aagtatagtt atcaagaatt cgtaaagcgt
    29761 aggaaacaat tccaacaaag agttgtcacg cagcgaagct tgcgtcaatt aaatcgaaca
    29821 attaatgcac atgaagaaaa ataccacgca agtaaagcat atgaagagca acagcgaaga
    29881 atccaaatgg aacaagaaag acaataggag gaagagaaat gaaacaatat ttagatcaat
    29941 ggaaagtcat agaaggatca ctaaaagaag aacgaatcga acaacttcct gattgtttag
    30001 aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa cgaacagttt gatccaaatc
    30061 aatttttagt tgttgagtac ccagctactg gtgtgtattg ttgtaatcat gtgaagggtg
    30121 aaaaatattt tattattcaa gaatacgaag gtaagttagc gccttattat actacttggg
    30181 aaataaatga agagggaatc aataatttcc catgtgaaag cattgaagag tctatttctc
    30241 tcacggaatg ttaaataaat agagacgtaa aagagtcctg aacgtgattg tccaggactc
    30301 tttttcaatg aattgttttc cgccaattca ctgagtggtg cgattcaatt ttattatatc
    30361 aaaagaaagt gagattccaa agtgaaggga ggaagaagaa atgactcaga caacgattgg
    30421 tgttgtggtt gaaaaaagca gggataatct cgtttttgtg acagaaattc aaacaggtag
    30481 agcttttgta gtcacagaca aagctgcaaa agcgtatcaa aatggcaata ttttaacgtt
    30541 aaatctgact acgaagatgt tcgttgatgc agtagaaaat tatccatttg tttagacaaa
    30601 cgatatttta caaggaggaa acggacatgg cagaagaaaa agaaccacta gataatagtc
    30661 ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa cgaattaaat aatcaatttc
    30721 aaaagctatt tgatcaccaa aaattgacaa atgggcaacc aatgaatgac aaacgaaatg
    30781 gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa aatacaaagt ttgagagaag
    30841 agatcaagca acaagaaaaa caggtagaaa aacttgaaag acaagaagag ctaaaggaaa
    30901 tgggctataa caagtacggc ggactggata tgacgatcga taatattcca agaatcaaag
    30961 aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgccacg atccgaaaat
    31021 atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa atcagaaaaa ggaaaagaaa
    31081 atatcctccc tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaaa
    31141 atccaaccat ttattttttg aaaggctatc gaaaagttgc tttagaactg aatgaaaacg
    31201 ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa gcagctgttc
    31261 gtgaattact tgaagaggct gcaccaactg attctctgaa ggagaaagaa aaagatcatt
    31321 tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt tatggtgggg
    31381 agatcgtaac attagaattg atcgatcaac ttaaaacgtt ggataaccaa tacgctgcag
    31441 aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta gtcgatcaga
    31501 tccgaatgga tctaggggat ggcgtcaaca gtaatcaccc catctattta gagttagcaa
    31561 aattgtgcga acaagtacct gagaaggaac aaaaacaatc ggtcaaaaat gaacctgttt
    31621 cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca aaagggctat caaaaacgaa
    31681 cggcagaaga gatcaaacaa gaagttaaat ccctttcgca acaggcactg gatgcggtga
    31741 aaaagcatac ccattcttct gaagacgtca aagaactttt gaactttatg tctcgttttc
    31801 cagaacgatc ctttagaaat caattattga ttgaggaaca atttccaggt gcaacggctt
    31861 gtttaggtcg tgccgcgttg aaaaaagaag gaatctatat caaaaaaggg gaacaaggga
    31921 acaagatctt tgtcagaaaa acggtcaaag gattttatga gaaaggacgt ggctttgttc
    31981 gagaaacaga agctacacca gaagataaaa aacgaatcga aagtggtcaa atcgaagtga
    32041 tcaaaaaa
//
LOCUS       DAZUKD010000022        30951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000022 DAZUKD010000000
VERSION     DAZUKD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.022"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..3800)
                     /gene="rpoC"
                     /locus_tag="ACG8N9_000866"
     CDS             complement(147..3800)
                     /gene="rpoC"
                     /locus_tag="ACG8N9_000866"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4896487.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3879..7496)
                     /gene="rpoB"
                     /locus_tag="ACG8N9_000867"
     CDS             complement(3879..7496)
                     /gene="rpoB"
                     /locus_tag="ACG8N9_000867"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4896488.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8224..9192
                     /locus_tag="ACG8N9_000868"
     CDS             8224..9192
                     /locus_tag="ACG8N9_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4896489.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9210..10115
                     /locus_tag="ACG8N9_000869"
     CDS             9210..10115
                     /locus_tag="ACG8N9_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896490.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10112..10918
                     /locus_tag="ACG8N9_000870"
     CDS             10112..10918
                     /locus_tag="ACG8N9_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896491.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11075..12043
                     /locus_tag="ACG8N9_000871"
     CDS             11075..12043
                     /locus_tag="ACG8N9_000871"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4896492.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12360..12884)
                     /locus_tag="ACG8N9_000872"
     CDS             complement(12360..12884)
                     /locus_tag="ACG8N9_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4896493.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13171..14982
                     /gene="scm"
                     /locus_tag="ACG8N9_000873"
     CDS             13171..14982
                     /gene="scm"
                     /locus_tag="ACG8N9_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4896494.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15137..16123)
                     /locus_tag="ACG8N9_000874"
     CDS             complement(15137..16123)
                     /locus_tag="ACG8N9_000874"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4896495.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16146..16712)
                     /locus_tag="ACG8N9_000875"
     CDS             complement(16146..16712)
                     /locus_tag="ACG8N9_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4896496.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16726..17142)
                     /locus_tag="ACG8N9_000876"
     CDS             complement(16726..17142)
                     /locus_tag="ACG8N9_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4896497.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17599..19533)
                     /locus_tag="ACG8N9_000877"
     CDS             complement(17599..19533)
                     /locus_tag="ACG8N9_000877"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4896498.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19835..20770
                     /gene="pplA"
                     /locus_tag="ACG8N9_000878"
     CDS             19835..20770
                     /gene="pplA"
                     /locus_tag="ACG8N9_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS4896499.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20860..21963
                     /locus_tag="ACG8N9_000879"
     CDS             20860..21963
                     /locus_tag="ACG8N9_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4896500.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21976..22929
                     /gene="menA"
                     /locus_tag="ACG8N9_000880"
     CDS             21976..22929
                     /gene="menA"
                     /locus_tag="ACG8N9_000880"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4896501.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23743..25089)
                     /gene="gor"
                     /locus_tag="ACG8N9_000881"
     CDS             complement(23743..25089)
                     /gene="gor"
                     /locus_tag="ACG8N9_000881"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4896502.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWKASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25223..26476)
                     /locus_tag="ACG8N9_000882"
     CDS             complement(25223..26476)
                     /locus_tag="ACG8N9_000882"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4896503.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26482..27405)
                     /locus_tag="ACG8N9_000883"
     CDS             complement(26482..27405)
                     /locus_tag="ACG8N9_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4896504.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27626..30118)
                     /locus_tag="ACG8N9_000884"
     CDS             complement(27626..30118)
                     /locus_tag="ACG8N9_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4896505.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(30125..30592)
                     /locus_tag="ACG8N9_000885"
     CDS             complement(30125..30592)
                     /locus_tag="ACG8N9_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4896506.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(30728..30801)
                     /locus_tag="ACG8N9_000886"
     tRNA            complement(30728..30801)
                     /locus_tag="ACG8N9_000886"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30765..30767),aa:Arg,seq:acg)
     gene            complement(30859..30932)
                     /locus_tag="ACG8N9_000887"
     tRNA            complement(30859..30932)
                     /locus_tag="ACG8N9_000887"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30896..30898),aa:Pro,seq:tgg)
BASE COUNT         8983 a         6592 c         5774 g         9602 t
ORIGIN      
        1 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
       61 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
      121 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
      181 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
      241 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
      301 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
      361 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
      421 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
      481 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
      541 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
      601 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
      661 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
      721 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
      781 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
      841 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
      901 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
      961 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
     1021 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
     1081 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
     1141 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
     1201 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
     1261 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
     1321 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
     1381 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
     1441 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
     1501 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
     1561 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
     1621 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
     1681 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
     1741 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
     1801 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
     1861 cctaagtctt tcatacggtc aagcatctta gaagtttctt ttactttgaa tcgtttgaag
     1921 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
     1981 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
     2041 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
     2101 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
     2161 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
     2221 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
     2281 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact
     2341 ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
     2401 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
     2461 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
     2521 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
     2581 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
     2641 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
     2701 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
     2761 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
     2821 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
     2881 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
     2941 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
     3001 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
     3061 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
     3121 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
     3181 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
     3241 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
     3301 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
     3361 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
     3421 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
     3481 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
     3541 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
     3601 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
     3661 aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
     3721 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
     3781 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
     3841 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
     3901 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
     3961 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
     4021 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
     4081 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
     4141 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
     4201 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
     4261 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
     4321 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
     4381 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
     4441 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
     4501 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
     4561 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
     4621 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
     4681 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
     4741 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
     4801 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
     4861 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
     4921 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
     4981 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
     5041 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
     5101 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
     5161 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
     5221 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
     5281 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
     5341 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
     5401 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccatc atgtttacat
     5461 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
     5521 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
     5581 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
     5641 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
     5701 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
     5761 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
     5821 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
     5881 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
     5941 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
     6001 gtcaatccgc caggtcctaa ggcgaaaaga cgacgtttgt gtgtcaattc gccaagaggg
     6061 tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
     6121 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
     6181 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
     6241 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
     6301 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
     6361 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
     6421 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
     6481 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
     6541 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
     6601 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
     6661 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
     6721 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
     6781 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
     6841 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
     6901 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
     6961 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
     7021 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
     7081 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
     7141 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
     7201 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
     7261 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
     7321 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
     7381 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
     7441 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
     7501 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
     7561 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
     7621 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
     7681 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
     7741 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
     7801 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
     7861 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
     7921 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
     7981 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
     8041 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
     8101 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
     8161 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
     8221 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
     8281 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
     8341 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
     8401 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
     8461 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
     8521 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
     8581 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
     8641 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
     8701 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
     8761 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
     8821 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
     8881 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
     8941 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
     9001 ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca
     9061 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
     9121 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
     9181 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
     9241 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
     9301 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
     9361 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttaggga
     9421 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
     9481 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
     9541 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
     9601 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
     9661 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
     9721 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
     9781 ctaatggatg cattgctctt tctggctcct tgcttgcgca aaacaacggc tttgctgatt
     9841 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
     9901 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
     9961 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    10021 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    10081 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    10141 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    10201 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    10261 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    10321 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    10381 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    10441 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    10501 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    10561 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    10621 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    10681 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    10741 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    10801 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    10861 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    10921 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
    10981 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
    11041 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
    11101 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
    11161 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
    11221 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
    11281 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
    11341 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
    11401 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
    11461 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
    11521 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
    11581 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
    11641 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
    11701 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
    11761 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
    11821 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
    11881 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
    11941 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
    12001 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
    12061 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
    12121 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
    12181 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
    12241 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
    12301 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
    12361 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
    12421 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
    12481 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
    12541 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
    12601 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
    12661 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
    12721 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
    12781 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
    12841 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
    12901 tatttatcct acttactttt tcctaattct aggataccta cttatatgtt actacacacc
    12961 cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga
    13021 tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt
    13081 aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg
    13141 aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta
    13201 gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt
    13261 cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt
    13321 gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga
    13381 aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt
    13441 ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc
    13501 atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca
    13561 cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat
    13621 ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg
    13681 aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat
    13741 tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta
    13801 ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca
    13861 aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca
    13921 ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc
    13981 tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca
    14041 atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga
    14101 acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc
    14161 gacaatggta cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat
    14221 agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac aagtgatagc
    14281 acggatacat cagcttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg
    14341 gacacatcag cttcaagtga cagtacagat actacctcta ccacaagtga cagcacggac
    14401 acgtcagctt caagcgacag tacagatact acctctacca caagtgatag cacggacaca
    14461 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagcac ggacacgtca
    14521 gcttcaagcg acagtacgga tacatctacc acaacaagtg atagcacgga cacgtcagct
    14581 tcaagtgaca gtacagatac tacctctacc acaagtgata gctcagattc atctaccact
    14641 acaagtgata gcacggacac gtcagcttca agtgacagta cagatacatc ctctaccaca
    14701 agtgatagct cagattcatc aacttcaagc gatagtacag acacgtcgtc taccacaagc
    14761 gatacacgtc atgcaagtgt ctccttctca aaaacaagtt cggataacag gacgaacttt
    14821 aacttaacta ataaaacaag caatgacaat ggaaacaaat caaaaagagc acttcctaaa
    14881 actggaagtc aatcgaataa ttggataaca ctcgcaggcg ttatattact tgtaatcggt
    14941 ttaagaatca cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa
    15001 aaagagcaac gtcgctaacg gacgttgctc tttatttttg ctttttatca taaatacaag
    15061 aactacaatt caatttgtca aaaaacacgt agatttcctt ctgagaaatt tatttacgta
    15121 tcgaaccttt ccctattcag tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat
    15181 tctcttttgt tcgaagagag gtggcaggta atttctttat ttccttcaaa gccttttctg
    15241 tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc tgtatgttca acgattttgc
    15301 gtacggcgtc gacatcttct tctgtcattg ccgctttctt ctctaaataa ggtagtagct
    15361 ttggtttgtt tttttgcaaa gagtagatca acggtaaaga atagacccct tgacgcatgt
    15421 cttctagtac aggtttgccg atttcctggc tctcttttgt ataatctaaa atatcatcga
    15481 taatctgaaa ggccatcccg atagaaagtc cgatatctcc tgcttttttt gcgaagcgct
    15541 cgcttgttcc actttcaaaa gcgccaacag aacaacttaa ttggaacagt tcagctgtct
    15601 tacctgaaat attttccaaa tattctttta cagaaagttc ataattatag cgatcatcca
    15661 tctgacctaa ttcgccattc aatacttttt ccatactgcg agaattcaat tggagacttt
    15721 tcaaggaagt ggagtaatca gacaatagtt taaaacagca gacaaataaa tagtctcctg
    15781 catagacaga aacgacatta ccaaactgac tacggatatt aggctgacca cgtcgcagat
    15841 ccgcttcatc aacgatatca tcatggatca gtgtggccgt atggagcatt tcgatagaag
    15901 ctgccaaagc gatggctttg tctcgatctt gctctggtcc aaattgtgag aacaatagct
    15961 gataggcagg acgcaacagc tttccgccgg catggatcgt ttctttgatt gcttgttcga
    16021 caggtttgtt tttcaggcgg acagtttctt ccatccgttt tagtgtttct gtcaattctt
    16081 tacttaaagc aggatagttt tcccacattg gatgtagctt cataaaaaaa ctccttgtgt
    16141 gacgattatt ttttggtaaa cattgggctc catttttgtt tggttccctg ctcataatct
    16201 ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt
    16261 gcaatccctg ataaaatccc taaaactgaa aggactggca agtacaacat gactgtccat
    16321 gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat tatgcatgaa accgccggcc
    16381 gcactaattc cgatgatact gacacgtttt ggtcctaatt gcttgaccaa taacattcca
    16441 aaataactaa gcaaagcacc actcatacta taaaggaatg tggaaatcgt tccgcctagc
    16501 aaagtagtca ggaatagtcg tagccaaagt agtaagaagc tgtctcgttt tttcatggta
    16561 aaaatggcaa cgatcgtgat tagattggct aagcctagtt ttgcaccagg cgcaaaagaa
    16621 aaaggataag ggatcatatt ttccaataaa ccaatgatca ctccttgtgc aacaagtagc
    16681 gaaatataaa tcattttttg taatttactc atggtgtgtg aaggtctaat aaatcagact
    16741 gtcttcgtca cctccatctg ttgaacggac ttcgatcact aatttatgtg gcaaacagac
    16801 gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg cagatctggt catcgcaatc
    16861 tgcttctttt atccgtactc gatcgccatc tatctcgatc aagttataat ctccgtgttc
    16921 atctttgtaa gtatatgtgt tgctctcttc tccagccatc aaagggaatg ttttgatttc
    16981 tgtgccatcc acacgcagta ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc
    17041 aaaaatcatg actggtaaaa aagaaagaac agtcaaaagc aaaataataa aaacatccca
    17101 aggtttgagc agactttttt cagcaaattc tttaaggctc atttttgttc tcctttttcc
    17161 agattaactc acccaatttt agcataaaaa caagaggttg tgacacctgt gtcttgcttt
    17221 gagtaataag aacgaagaat agaaaacagc tttccatgtt ttctaattct ttgggcttat
    17281 taccgagaag cagcaggtgg aacaccgttt ataacaagag gttgtgacag aagtgggcaa
    17341 cttcaagaaa taagaaggaa tttacgaaaa ttacttttca agtttttgtg aaatttcggc
    17401 ttatttttcg aagaagctac ttctggaaca ccgtttataa caagaggttg taacagaggt
    17461 tgtgacacct gtgtcttgct catggaggct tatctcgggc atcttattcc tttaatttta
    17521 cataaaaagg aggctgtgac ggtttgtctc agcccctttc tattgaaatc ttgcattcaa
    17581 ataacgttct ttatctattt atttcttgcc gtactgggaa cttggtctac cgtaccacca
    17641 attacctagt gtacgttgta gccatggaat tacccagcga tccaacccaa tcgcacgtcc
    17701 ggaaccgttc atcaatgcga aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat
    17761 gcctgataag cagaacgtga tcaccaatac aatcgttgtc gcactgcata gccatgtgaa
    17821 taatccagca atcaatgcta atgccaaggc aatttcaaag aataccatga atttttggaa
    17881 gaacattgct acatcattgt ttggcatgat cacttgcatg atctttgtga accagtctgg
    17941 tgacttctca atcacagcca ttggttcttc accgtatgca taactcaaac caaaatgtgc
    18001 agcttgagtc gctgcttctc cggcacttgc ccctgctccg ctggcagctg tggttgcatc
    18061 accagcagca gaagcaccac tggcaacatc tgttacattg tattcccatg tccaagggaa
    18121 cacactgttt ccagtgaacc atgatccttc accaaaccaa gtacttggtt tgaacacttc
    18181 accattccca atgatttttt tggaagcttc aacgaaccaa actaaaccat agaaaatacg
    18241 aagtggcaca ctccataaga cattgccgta acgagaagaa tgtcctcgag tcacgttgcg
    18301 atcatctttg atatggaaaa actcatgcat taaatattgg aacatataat aaccagaacg
    18361 aatatcaaag aagtatttca aattcacgat atgtttcatt acgatagcta aaaatccgct
    18421 taaatggatc ttatcaaata ggttagctac cccccatttg gcaccaatag aaaccataaa
    18481 tccttgatag tttcctttaa atttatgctt ttctccgcct ttgatcgaag ccacgatatt
    18541 cgctgcggct gtgtgtcctg tttgttcagc agcttgaacg atctgcggtg tcggtgttcc
    18601 tgggaattct tcataaaaga ctaaatcacc aatgatatag atgtttttat cttcgtagcc
    18661 ttttgcctgc atatattcat tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa
    18721 gtcagcagcg tcagaagttc ctttgacacc agctgtccaa atcagtgtgt gagtcggaat
    18781 gttgcgtccg tctttcaatt tgatatgatc agcagccact tctacgattg gtgcattcaa
    18841 taataattcc acgttgtgct tacgcaaata gcgttcagct tttgcagcat catttcgatc
    18901 caacatattc aagatcgtcg gcattgcctc tacgaccatc agtgtgattt cgtttggatc
    18961 taatttataa tctttggcta ggcgattttt ccagtcgatc agttccccaa tcatttcaat
    19021 accggtaaat ccagaaccac agacaacgaa agtcagcatg gctttacgca gttcatcatc
    19081 tggttccaat gcagcttttg ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc
    19141 ttcaaatgac caaagggtga atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg
    19201 ctctccgccc attccaagga tcagctgatc aaaaggatag gaacctaatt tggtcttgac
    19261 gatttttcgt tctttatcga taccgattac agtgtctgtg acaagttgta cattcttttg
    19321 acgagcaaac aaatgctgca gatcgtattg gattgcttct ggttctacac gtccgccagc
    19381 tacttcatgc aactcggtca tcatagtatg gtacgaatga cgatcgatca gagtaattgc
    19441 tacatcttct tttttcagtt tctttgccaa gtattttgta gctgaaacac cggcatatcc
    19501 tgctcctaca acgacaatat tttgctttgc cattgatcat ccgctcctta tagactatct
    19561 atataatgaa tatatgataa ttcttttagt ttgatactta ttacacaatc tccatatatt
    19621 atatagatgt tctgttcttt tgtctaactc cttttggatt tttttcaaat tttaaaataa
    19681 agcgtttttt cacgaaattt gctgaaagaa aacgtttgaa atattacatg ggttatttat
    19741 caaaaatagt tgacttatag gctaaaaaga ttagaatgta taatgaatca caaagaaaaa
    19801 ttgtggacta gaaatggaat ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg
    19861 atttgccgta ttagcatttt cagctctagt gttaggcgct tgcggcgcag atgacaacgg
    19921 aagctcaaac tcttcttctg aatcatctac agcacaatct tcgactgcaa aaagcacaac
    19981 agaatcatct gcaaaagttg tagctggcgg agatttgcaa gacggcacat ataaattaga
    20041 agaaaaaaat tattcaaatg gttaccgtgc agtctttgaa atggttgtaa aagacggcaa
    20101 aatcactgaa tcaaaatatg ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc
    20161 agaatacaac aaaaacatgg aagcaaaatc tggcacaaac ccagaaaaat acattcctga
    20221 gttgaatgac caattcttaa aagcacaaag cgcaagcggt gtggaagtcg taactggagc
    20281 tactcactca tctgaaagct tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc
    20341 tggtaacaca gatacaatcg aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa
    20401 gcttgaagaa aagaactact ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg
    20461 cggcaaagta actgaatcaa actttgatta tatcgacaaa gacggtaaat caaaacaaga
    20521 tgatacagaa tataatgaaa atatgaaagc aaaaagcggt acagaaccaa aaacttacat
    20581 ccctactctg aacgacgaat tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa
    20641 tgtagaagtt gttacaggtg ctacacacag ctcacattca ttcatcatgt acgcacaaca
    20701 attagtgaat gcagctgaaa aaggcgacac tcaaacaatc gaagtagata atatcgtaac
    20761 agaaaaataa tcacataatc atactaataa aagttggagg gtgggacgat agccagatta
    20821 ggcagtcgtc tcacctttca aaatgaagag aggaaaaaga tgcggaaaaa actactattt
    20881 tccttaatca cactgggcat ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat
    20941 aaaacaacca ctgaaagttc agcagctacc aagatattga aagatccata ttctgatgaa
    21001 cagttcttgt tagggactta cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca
    21061 ttgaaacctg ccttcgatcg agtaaaagaa cttggtgata aaatcacgat caatcaaaaa
    21121 ggttctgaga tcgatgaagt gaatgagcaa gctgggatca aaccagtaaa agtttctgat
    21181 gacgtctaca cgttagtcaa gcgggcctac gaatatagcc aagattctca aggcggattc
    21241 gatatggcga ttggtgcaat cacacaatta tggcgaatcg gttttgatga cgccagaaaa
    21301 ccttcccaag aagaaatcga tcaggcattg aaattagtgg attatcataa gatcgaacta
    21361 aatgacaaag aaaaaactgt ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca
    21421 atcgccaaag gttacattac ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc
    21481 actgccatcg ttgacttggg cggtaatgtc tatgtcttag gccacagccc aagaggcgaa
    21541 aaccaagact ggacagtcgg catccaagat ccgaacatgg ctcgcggatc tgttttaggt
    21601 tctattaaag aacggaacaa aacattagtt acctctggaa tttacgagcg ttatttagaa
    21661 gtagatggca aaaaatacca tcatttgttt gatccaaaaa cgggttatcc gttcgacaat
    21721 gatatcgcaa gtgtcacgat cataacagat aaatcaattg atggtgacgg gttgtctaca
    21781 gcagtctttt caatgggtgt gaaaaaagga ctggaatacg tcgaaagcga attgaataac
    21841 gggacggaag cgatcttcgt gacaaaagac gataaagtat atgtgaccga tccaatcaaa
    21901 gacaccttca aattatcgga ggactcccat tacacgatgg gtgaccgttc tgaactaaaa
    21961 taaggagtac gaaccatgac actaaaaatc tttttagaag tagttgaaat acgaacaaag
    22021 gtggctagtg tatttccttt tgcggtaggg gtattattct ctatcgcttt ttttcatgag
    22081 atacattggc taaacacttt tttatttttt attggaatgc tagtttttga tttagcgact
    22141 accgctatca ataattacat ggattatgaa aaggctcatt ctgaagtata taagtatgaa
    22201 gaaaatgtca ttggccgcag aaatctatct cctgtattga ttcgaaacat gattttagct
    22261 atgattgcat ttgtttttgt tatcggcgtt attctaacga tcaatacggg ttggctttta
    22321 cttttgatgg gatttatctg ttgttttatt ggagtattct atacattcgg accgattcct
    22381 ttatctcgaa tgcctttagg ggaaattttc agtggggtga ctatgggcct tggaattttt
    22441 gccatggtcg tttatgtcaa tacattcggt tcagatgtct atgatctgac agtcaacttt
    22501 tcatctggac aatttgcttt ggtaggaaat ctttgggcta tagcagcaat cgtattagct
    22561 tccttaccct tggtgtttac gattgccaat atcatgttgg ctaataattt gcgggatctt
    22621 gaccgtgata ttgaaaatca tcgctataca ctagtttttt atatcggacg tcctgcggga
    22681 acagtcttat tccagctatt gatgtatgct tgctatttcg tgattttaat ggggttgttg
    22741 agcggtatct atgaatggcc gatcttggtc accttcttga ccttgccaat tgtgaagaag
    22801 catttggatc aattcaagca aacgttaccc cagcctatca gtttcagcta ttccatcaaa
    22861 aatatggtct tattcaatgc cagttatgta ttggggctat tactaacaat cattatgcaa
    22921 gccatttaac agcatgaaga ggttgtgaaa aaactgccct gcatcaagta ataagaacag
    22981 ccaaataaaa atagctcctt atatttttcg tttggttggg cttattaccg cagatgcaag
    23041 cttttgaaca ccgtttatca ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa
    23101 aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact
    23161 ggcttttgaa caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag
    23221 aaacaaatgg aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca
    23281 gctgattgaa cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag
    23341 tccctttttt cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg
    23401 gtaactatat ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag
    23461 tcacactttt ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc
    23521 aatattttga acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa
    23581 gataatcaaa atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt
    23641 acataaataa ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca
    23701 tggtttgttc aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa
    23761 ttcttccgct cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc
    23821 catcttgata gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg
    23881 taagccgacg attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg
    23941 ataatcattt aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt
    24001 ttcgccatat ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg
    24061 cgtaaagacg actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa
    24121 taaacgttct gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc
    24181 gatcacatca ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt
    24241 gataaaccct tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt
    24301 cggtacacgt ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa
    24361 tagcaccgta tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt
    24421 ttttattcca gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt
    24481 tctaagtggt cgttcttttc gatatgccca catgacttca ctacctaaac cacaaaatac
    24541 acctgaaagt tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc
    24601 tgttaattca aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt
    24661 acttgcacgg cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac
    24721 ttcgatcgtt tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct
    24781 atctaatcct ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac
    24841 tagaccggcg aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg
    24901 ttcaattgtt tccaaaatcg tacttgcttt ccacataact tttttaggta cacaaccgac
    24961 gttgacacat gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat
    25021 ccctgctctg tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata
    25081 tatttgcatt ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct
    25141 ttttctacag aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa
    25201 cagggatata tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg
    25261 gctgtgcgac tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc
    25321 cttcttcatt atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa
    25381 attcgatttc atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg
    25441 cctcatcata agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac
    25501 cgaataattg ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt
    25561 gtgctacttt ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt
    25621 ccatataact gtctacagcc gctttataga catttgacac cgtagataca taatggatcg
    25681 atttcattct tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat
    25741 gttcgatcat tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat
    25801 cagttaaact gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac
    25861 aagattgaga acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc
    25921 atcgaccaga ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg
    25981 tatttttacg aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt
    26041 ccaacccttc ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg
    26101 ataaatggat cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag
    26161 aaacaatcac tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat
    26221 cttcatttcc ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt
    26281 ttgcaaaagc aactccttca gccatttctt cataagtaaa attacctgca cggcttctta
    26341 aaccatatgc attcccgcca atatatactg catctgctcc ataatggatc gcggttttta
    26401 acttttccag tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg
    26461 tcatttcttt catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt
    26521 atctaattca cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt
    26581 ttcttgatcc cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat
    26641 gtttacgaat ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg
    26701 ctgataaagt tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt
    26761 gccatgacta tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa
    26821 aagacctctt tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag
    26881 cagtggacgt ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat
    26941 atcgagtttt tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc
    27001 tccaactgcg ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc
    27061 agcatcataa atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc
    27121 tgtatcacca actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg
    27181 tacatttttc attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc
    27241 atacttgtgt gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag
    27301 acgcaatcca aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg
    27361 ttctgcttgt tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta
    27421 caacttcata atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg
    27481 gatttttgaa aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata
    27541 taaaaagaga ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa
    27601 aaattcagct acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc
    27661 gaacattcaa tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa
    27721 tttctccgcc aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac
    27781 ggattggacg tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag
    27841 atggtgtgat tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca
    27901 tgatttggac gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat
    27961 cgattcgatt caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt
    28021 gcatggcttt gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc
    28081 gaatttgtgt cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt
    28141 ttctaccttt agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa
    28201 cgtctggatg tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa
    28261 ttttttctgt caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta
    28321 atcggcttgt actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt
    28381 cacttccaaa catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg
    28441 gtccaaggaa catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac
    28501 ggcggatcgc acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca
    28561 gaagtccttc cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga
    28621 caccagtcca ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac
    28681 cttgctcctg ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt
    28741 cttgtctgcg taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt
    28801 cttgtactag ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt
    28861 gatccaaacg gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt
    28921 gacgagaagt gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga
    28981 tctccactcc atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt
    29041 cttcaggagt tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt
    29101 tttcgatata tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc
    29161 gagctaacgc cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac
    29221 caattaaggt atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt
    29281 cgatgacctt tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag
    29341 atcccatatc aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa
    29401 tgattttttg agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca
    29461 gtacagggtt attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg
    29521 aacgaccaac aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta
    29581 atgaatctag cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc
    29641 tacgacgatt cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa
    29701 cttgacgggc ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac
    29761 tttcatcttt taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt
    29821 tcttcacttc gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa
    29881 agactccttt agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat
    29941 aaatttccgt ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct
    30001 cgaccactaa ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg
    30061 cttcttcttg tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct
    30121 aatctcactt cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac
    30181 gtgtatgatc ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca
    30241 agtttccttc tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag
    30301 catttttctc actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt
    30361 tatctgaaat ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta
    30421 ctagataacc acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa
    30481 cacagttaaa gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca
    30541 gaattttctt tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca
    30601 ttccctttga ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa
    30661 cgctttttgt taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc
    30721 gtatcgacgc gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct
    30781 atccagttga gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa
    30841 aaagcaagat aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa
    30901 ccaagtgctc taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DAZUKD010000023        30097 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000023 DAZUKD010000000
VERSION     DAZUKD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.023"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..2436)
                     /locus_tag="ACG8N9_000888"
     CDS             complement(73..2436)
                     /locus_tag="ACG8N9_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFS4896507.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2601..3105
                     /locus_tag="ACG8N9_000889"
                     /pseudo
     CDS             2601..3105
                     /locus_tag="ACG8N9_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3213..3560)
                     /gene="rbfA"
                     /locus_tag="ACG8N9_000890"
     CDS             complement(3213..3560)
                     /gene="rbfA"
                     /locus_tag="ACG8N9_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4896508.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3587..5893)
                     /gene="infB"
                     /locus_tag="ACG8N9_000891"
     CDS             complement(3587..5893)
                     /gene="infB"
                     /locus_tag="ACG8N9_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4896509.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5906..6217)
                     /locus_tag="ACG8N9_000892"
     CDS             complement(5906..6217)
                     /locus_tag="ACG8N9_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4896510.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6214..6507)
                     /locus_tag="ACG8N9_000893"
     CDS             complement(6214..6507)
                     /locus_tag="ACG8N9_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4896511.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6529..7704)
                     /gene="nusA"
                     /locus_tag="ACG8N9_000894"
     CDS             complement(6529..7704)
                     /gene="nusA"
                     /locus_tag="ACG8N9_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4896512.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7728..8201)
                     /gene="rimP"
                     /locus_tag="ACG8N9_000895"
     CDS             complement(7728..8201)
                     /gene="rimP"
                     /locus_tag="ACG8N9_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4896513.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8345..12697)
                     /locus_tag="ACG8N9_000896"
     CDS             complement(8345..12697)
                     /locus_tag="ACG8N9_000896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4896514.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12909..14618)
                     /locus_tag="ACG8N9_000897"
     CDS             complement(12909..14618)
                     /locus_tag="ACG8N9_000897"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4896515.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14686..15954)
                     /gene="rseP"
                     /locus_tag="ACG8N9_000898"
     CDS             complement(14686..15954)
                     /gene="rseP"
                     /locus_tag="ACG8N9_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4896516.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16115..16915)
                     /locus_tag="ACG8N9_000899"
     CDS             complement(16115..16915)
                     /locus_tag="ACG8N9_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4896517.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16912..17724)
                     /locus_tag="ACG8N9_000900"
     CDS             complement(16912..17724)
                     /locus_tag="ACG8N9_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4896518.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17919..18476)
                     /gene="frr"
                     /locus_tag="ACG8N9_000901"
     CDS             complement(17919..18476)
                     /gene="frr"
                     /locus_tag="ACG8N9_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4896519.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18479..19201)
                     /gene="pyrH"
                     /locus_tag="ACG8N9_000902"
     CDS             complement(18479..19201)
                     /gene="pyrH"
                     /locus_tag="ACG8N9_000902"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4896520.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19337..20218)
                     /gene="tsf"
                     /locus_tag="ACG8N9_000903"
     CDS             complement(19337..20218)
                     /gene="tsf"
                     /locus_tag="ACG8N9_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4896521.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20317..21099)
                     /gene="rpsB"
                     /locus_tag="ACG8N9_000904"
     CDS             complement(20317..21099)
                     /gene="rpsB"
                     /locus_tag="ACG8N9_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4896522.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21458..21937
                     /locus_tag="ACG8N9_000905"
     CDS             21458..21937
                     /locus_tag="ACG8N9_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4896523.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22154..23245
                     /gene="phnW"
                     /locus_tag="ACG8N9_000906"
     CDS             22154..23245
                     /gene="phnW"
                     /locus_tag="ACG8N9_000906"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4896524.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23238..24023
                     /gene="phnX"
                     /locus_tag="ACG8N9_000907"
     CDS             23238..24023
                     /gene="phnX"
                     /locus_tag="ACG8N9_000907"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4896525.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24369..26060)
                     /gene="argS"
                     /locus_tag="ACG8N9_000908"
     CDS             complement(24369..26060)
                     /gene="argS"
                     /locus_tag="ACG8N9_000908"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4896526.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26482..27429)
                     /gene="arcC"
                     /locus_tag="ACG8N9_000909"
     CDS             complement(26482..27429)
                     /gene="arcC"
                     /locus_tag="ACG8N9_000909"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4896527.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27544..28563)
                     /gene="argF"
                     /locus_tag="ACG8N9_000910"
     CDS             complement(27544..28563)
                     /gene="argF"
                     /locus_tag="ACG8N9_000910"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4896528.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28654..29883)
                     /gene="arcA"
                     /locus_tag="ACG8N9_000911"
     CDS             complement(28654..29883)
                     /gene="arcA"
                     /locus_tag="ACG8N9_000911"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4896529.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8036 a         6463 c         5351 g        10247 t
ORIGIN      
        1 agttttgtct cacttaattt gacaaataat acaatgtaaa aaattctgtt ttgcgggatc
       61 gattttatca ttttattggt caagattttt ccatttccaa tccaatactt ctggcagatc
      121 aataccattt tcacgaatat agttttgatg ttcttgtaat ttctgatcca ttttttccgc
      181 aaaatcttct gcttgacttc ctttgatttt taaagctgcg tcttttgcca aatgataacg
      241 atcaagttca tttactacac gcatgtcaaa tggtgtcgtg atatctccat tttctttgta
      301 gccatgaatc attaaatgat ggttatgacg atcaaagaaa atcgtgcgga ccaatgtttc
      361 ataaccgtgg aaagcaaata agatcggttt gtcttttgta aaatattgat caaattcttc
      421 atcgctcaac ccacgcggat cttgacttgg atgacgtaat ttcaataaat ctacgacatt
      481 gatgaaccga atttttagtt ttgggaattc ttccagcagc aaagttacag ctgctaatgc
      541 ttctaagtta gattccgttc ctgcagcagc aatgaccaat tcaggttcct cctcttttgc
      601 tgtcgaagcc caatcaatga ttttcaagcc ttcattcact aagacggctg cttcttctgc
      661 tgagtaaaat tgagcacgcg gatgtttgga agagatgatc aaattgattt tttctgacga
      721 gcggaatgct ttatccatga ctgcgagcaa tgtatttgca tccgcaggca gatattcacg
      781 gatgaactcc gccttctttt ctgccaaatg tgtcaatatt cctggatctt ggtggctata
      841 tccattgtga tcttgctgga agactgttga gcttgcaatg agattcagcg aaggataatc
      901 ttttcgccaa gaaaggtcat gtgatttccg tatccatttg aagtgctgag tcaacataga
      961 gtcaaccact cgcaagaatg attcgtaact tgcaaaaaaa ccatgccgtc cagtcagaac
     1021 gtacccttct aagaaccctt catcttggtg ttccgataat tgactgtcga tgacgcgacc
     1081 agatggcgaa agccattcat cattttctgg ttcgattttt tccatccatt ggcgatttgt
     1141 cgttttgaaa acttgattca atcggtttga ttttgtttca tctggtccaa atatacggaa
     1201 attttctgga tttttctcaa tgatctcgcg aacgaattta ccaagttcgg tcatatcttc
     1261 tgctttaacg gagcctggtt tttcaaattg caaagtatat tttttccaat ctggcataat
     1321 aagcggtctt ggatcgattc caccattggt gataggattt tttgccattc tttggtcacc
     1381 agtaggcgca atttctctga tttcttctaa aatacgtcct tgtgcatcaa ataatttttc
     1441 tggttcatag gatttcagcc aacggagtaa agcatctgca tgatccatat gttcttgatc
     1501 tacaggaatc ggtacttggt gtgctcggaa ggttccttca attggttccc cgtcccattc
     1561 ttttggaccg gtccatcctt ttggtgtgcg tacgatcaat actggccatt ttggcatcac
     1621 gacatctgtt gctgtcttca ggcgagcttc tttttggatg gatttgattt tctcgattgc
     1681 ttgatccatt ttttcagcca taacaggatt taatttttct ggatcatttc cttcaataaa
     1741 gaaaggctcc cagccaagtc cgttaaagta atttgctaat tctccatctg atttacgtgc
     1801 caagatcgtt gggttggcta tttttgcacc atttaaatga aggatcggta aaacagctcc
     1861 atctatcact ggattgataa agacatttga gaaccagctt ccagctaaag gaccggtttc
     1921 tgcttcacca tcaccaatca cgacagcaga gattacttca ggattgtcta atacagctcc
     1981 gactgcatgt gaaagagagt atccaagctc tccgccttca tggatagaac ctggtgtttc
     2041 tggtgctgca tgagaagcaa tgcctccagg aaatgaaaat cgcttgaata atttttgcat
     2101 tcctgtctca tcttccgtta cttctggata gatctctgtg taacttccat ctaaataagc
     2161 attcgatacc atgacttgtc cgccgtgtcc tggcccttcg atataaaaca tgttcaaatc
     2221 atatttattg atgactcggt tcaaatggac ataaataaaa ttttggccag gaatcgttcc
     2281 ccaatgaccg attggatgtt ttttgacatc ttctggtttc agtgtacgtc tcagtaaggg
     2341 gttatcttta agataaagtt gcccaacaga aagataatta gcagcacgcc accaagccgt
     2401 cattttatca aagtattctt tactagaata atccatacat aacactcctt ttcattatta
     2461 ctcttattct agcgcagata ctcccaattg aaacctaata agcacttctg ttcatttgtt
     2521 tggattttat atctagccaa atgaacaggt ataaaaaacc acgaaatttt tcctttcgct
     2581 gacgtttttt acattgatcc aaaaaacttg tcgttatcac aatggctgtt ccgcagttca
     2641 tttcgctcct catcatgcat aatttgctga atgacgctag gccagttaac acattgttgg
     2701 aaaactgggg ctggatcgag caattccttt cctgattgat ccgttttatg taaaagtctc
     2761 tgttatcttt gtcaatatgt gaattggtat cccagctact atgctaattt ctacagggat
     2821 tatccaaaat ttggatcgtg accaaatcga agcggcgtaa tcggaaatat caataacttc
     2881 aacgtgattt ttcttttaac tgtcaacgga ccgacaaact caaactttta tggtgcaggt
     2941 tccactgatc ttctagttac ttggctatac ctgactgtca ctaaaataga ttacaatcta
     3001 gcttccgtta tcggtatctt gatctttatc tatatgcaaa aatttatata ctatagaaaa
     3061 aagaaagtcg aattttttac ttttcttttt ctcttttttg gttagctgac acgctaccag
     3121 actaaagtca tggaactctt aggtaatacg tcttaatgaa aacaaaaaaa tcccgcaacc
     3181 cctgatttca caggatttgc gggatttttt gattagtctt tgttcaagtc gcgaagcaat
     3241 tcatcgatat ggttcccata gacaactgat tcatcaagtt caaagaacaa ttcaggtgtc
     3301 ttataaatac ttagattgtg ccctaactct cgacgaatca atcctgatgc tttattcaat
     3361 ccatcttgtg cttttttctt atctgatgct aagtcagata aaatgctgta atagatcgtt
     3421 gcttgctgta agtcccccgt aactttcaca tctgtgatcg ttacgttctc cacacgaggg
     3481 tcacggacct ttttgcgcaa aatatcattg acttctttta agatttcttg accgactcgg
     3541 cgatcacggt agttagccat aatgattggc ctccttttat cagtttttag tcatttttga
     3601 cttcttccat gataaatcct tcaattacgt catctacttt gatgtcattg aatttttcaa
     3661 tcatcgcacc acattcataa cccattttga cttctttcac gtcatctttg aatcgtttca
     3721 gacttgcaag ttggccttca tagattacga ttccgtcacg gattacacgg acaccgctat
     3781 ctctacgaat atatccttct gttacatagg cacctgcaat cgtaccaact ttagaaactt
     3841 tgaaggtttc acgaacgatc atttgaccgg tgattttttc ttcaaattca gggtctagca
     3901 tccctttcat cgcagtttcg atttcttcga tggctttata gatgatacgg tgaagacgaa
     3961 tatcgacttc ttcagtatca gcttgcactt ttgcctgcgg tgtaggacga acgttgaaac
     4021 cgatgataat agcgttgctg gctgctgcta aggttacatc actttcattg attgctccga
     4081 cagctgaatg gacgattttt acacggacac cttccacatc aatcttcttc aatgatgcag
     4141 ccagtgcttc tgctgaacct tgtacatctg ctttgatgat tacgttaact tctttcaatt
     4201 cgccttcttt caggctttca aataagttgt ccaaagtcac acggcttgtg gcagcacggt
     4261 tttccaataa tgcacgtttt gcgcgttctt cacccgctgc acgtgctgtc ttttcgtctt
     4321 cgaagactac gaaacggtca cctgcttgag gtacatcatt caagccagtg atttctactg
     4381 gcgttgcagg tcctgcttct ttatcgcgac ggccaagatc gtttgtcatt acacggacac
     4441 gtccgtaagt attaccgaca acgattgggt caccaacgtg aagtgttcct tcttggacaa
     4501 gtaaggttga cacaggacct ttacctttat caagacgtgc ttcgatcact gttccgattg
     4561 cgcgttgtgt tggatctgct tttaaatctt ctacttctgc gactaacagg atcatttcca
     4621 gcaattcttc gatgttttgg ccgaatttcg ctgagatctc aacaaaaatc gtttctccac
     4681 cccatgcttc aggaattaat tcatactcac ttaattcttg cataacatgc tgaggatttg
     4741 cacctggttt atcaatttta tttacagcga cgatgattgg aacgcctgca gctttggcat
     4801 ggttgattgc ttcaactgtt tgtggcatga cgccatcatc tgcagcaaca actaagatcg
     4861 tgatatctgt gatacttgct ccacgtgcac gcatacttgt aaaggccgca tgtcctggtg
     4921 tatccaagaa agtgattggt ttaccatcaa tatcgatctg gtaggcaccg atatgctgtg
     4981 tgattccgcc ggcttcgccg cttgttacac gtgaatggcg caatgtatcc aataaggttg
     5041 ttttcccgtg gtctacgtgt cccataatcg ttactactgg cggacgtgag actaatttat
     5101 ctggatcgac ttcttctgcc tcgaagaatt tatcgatatc agcaatgtca acttggactt
     5161 tttcttgtgg ttccatgcca tagtccgtcg ctaacagttc aatcgtatct ttatccagtg
     5221 cttggttttg attgaccatg acaccaagca taaataattt tttaatgatt tctgctggct
     5281 cacgatgaat ttttttcgca atatctgcta cgttcatacc ttcagtatac tctaatacat
     5341 ctggcaattc acggaatttg cgtggtggta cagctggttt agttgattgt tgctgtttgc
     5401 cttttttacc ttttttatta taacgattac ggttattatt attaaatttg ccgcgattgt
     5461 tattgtttgg tttgttgtta ttgttagtac ggttttgatt gccgccttgg ctcgttcctt
     5521 gacgattttg gctgctggta tttcccgtat tattttggtt tgttgttggt ttttttcctt
     5581 gattcacttg accgctccct tgaccgcgat cttgataact gcggttattt ttattttttt
     5641 gttgattgtt cgttgtttct tttttacgtt gatcgttcgg ctgttgattg gctggttttc
     5701 tgcttgcttg ttgtgcagct tgttgtttcg tgcttgggtt tttgaacgct tgttgcaatt
     5761 tcttctcatc actgctagta atcgcaccca tatggttttt cacatcgatg ccaagttgtt
     5821 gtgctttttc cacgacgtct ttactagatt ttttgagttc ttttgctaat tcatagattc
     5881 ttttgttgcc catgcaatca ccttcctaac cttcaattag ctccttggtc ttcttcgcaa
     5941 atccggcatc cattattccg aataccttgc gaggttttcc gatcatctgc gtgatttctg
     6001 cttcagaaaa tagctcaaag caaggaactt cataatatga actcttatct tttatctttt
     6061 ttcttgtatt ctcacttgca tcggttgcca caaacaccaa ttttgctttt tgtttgcgga
     6121 tttcagcaat ggtcagttct tcacccgtga ttagttttcc cgcgcgcatc gctagaccga
     6181 tgagattcat ggctttttgc cgattaactg tattcatttt ccaaataact ctttacgtgc
     6241 tttttggtga gtgacatagt caagaagttc ttgataaaac tcatctgtta atgttgtttc
     6301 gagtactcga tcgagaattt ttttatccca agcttgttgt acttcttccg gctcaagtga
     6361 gacatacgct cctcgtccag gcatttttcc agttggatcg atcgatactt ccccttcttt
     6421 tgaacgggtg attcggacca tctctttttt aggtttcatt tcacctgaaa ccacggattt
     6481 gcgtaaagga attttccttt gtttcatctc tttgcctcct tactaaagtt aagcttctgg
     6541 tttttcttcg accgtttcat tgttgaatgc aattgtttca tattcatcgt ctgttaaatc
     6601 tgactgaacg attgcttcat catgcagttc ttctgtttct ttttgttgtt cttgattttc
     6661 ataaaattcc gtcatgtcag attctgattt gatatcgatt ttatggttag tcaatttagc
     6721 agctaaacgt gcattttgtc cgcgtttacc gattgctagc gataattggt agtctggaac
     6781 gacaactgtg caggcctttg gatttgtctc atcgaagatc acatccatga cttgtgcagg
     6841 attcaatgca tttgcgatga atacagctgg atcttcattc cattcgacga tatccatgtt
     6901 ttcgcctttt aattcattga cgattgcttg aactcgttgt ccctttggcc cgacacatgt
     6961 accgactgca tcgatatttg gatcatttga gcgaactgcc acttttgaac gatcgccagc
     7021 ttcacgggcg atgctgacaa tttcaacgat tccatcatag acttctggta cttcttgttc
     7081 aaataagcga cgcaataggt ctggatggct gcggctaaca aatacttgcg gacctttaga
     7141 cgtattttct actcgggaaa cataaacttt gattcgatca tgtggttgat aaaattcatt
     7201 tggcatttga tcttgtttag aaagaactgc ctcaatttta ccaagattga cataaatata
     7261 acgtttatcc tggcgttcga cgattccttg catgatgtct ttttcataag cactgaattc
     7321 gttataaata atcgttcttt ctgcttcacg tacacgctgt aaaataactt gtttagccgt
     7381 ttgcgcagcg atccgtccaa aatcttttgg tgttacttca aaacggatcg tatcaccgat
     7441 ctcatatgct ggattgatta aaagtgcatc tttcaatgag acttccaact gtgaatccat
     7501 cacttcttcc gttacttctt ttaccgcata gacatggatc tttccttttt tctgatcaaa
     7561 ttccacttcg acgttttgtg cttggccata gtgacgtttg tatgcagaaa ccaatgctgc
     7621 ttctaaagca tcgatcacga tttcttttga gatccctttt tcagcttcta aagcgtctaa
     7681 cgcatttaac atttctttac tcattttttt ctgattcctc tccgtgatta aaattggata
     7741 gctaagcgag cttttgcaat attttttcgg tcaaaagtca attctttttc tcgtgttttt
     7801 atgcgtattt tcaatgtaag ctgttcagca tcaaatgatt gaagaaatcc ttcatattgt
     7861 ttttcgccat ctactggttg gtataaggag acatggacat aatcaccaag tgccttttca
     7921 tagtcgcttt cttttttcaa tggccgctct gctcccgggg aggatacctc caggaaataa
     7981 gcttgaggaa tcggatctgg atctgctgca tcaagttttt cactcagttt ctcactgaca
     8041 aaggcgcatt cttcgatatc gataccgcct tctttgtcaa taaatactcg taagaaccag
     8101 ctttttcctt cttttacaaa ttcgacttcg acaagttcaa aattttgttc atctaaaatc
     8161 ggtgtcacca tttctgtgac tgtttcaacg acgctactca aaagtttcgc ctccttaaaa
     8221 attaagtgcg taattccatt aaaaagagtg agcgaaaaat ccgctcactc acagttagta
     8281 tcattaccta taagaactat accatactct gcttaaaatt acaagcagac aggaaaacag
     8341 atgcttatag catatcgaat aacgataatt gattttcatc tggtagatct ttcaatacgc
     8401 cattgtcgtt catatattcg atcaatgttt ttgaaacttt accgcgggtg gcaagatctt
     8461 ctttggataa aaatggaccg tcttttcttg cttcaacgat ttgtttcgcc acgttggttc
     8521 ccaaacttgg gacagctcta aatggtgcga tcaacgtatc accttcaata acaaagttaa
     8581 cggcatccga tttatacaaa tcaatcattc caaatttgaa tcctcgttcc agcatttcat
     8641 tggctaactc cagcactgtc agttgattct tttcttttac ggaagcatct aatcccttgt
     8701 ccgtgatttc tttcattgct tgtttcacag catcttttcc tttgcacatg gagactagat
     8761 caaagtcgtc tgcacgtaca gaaaagtatg cacagtaata caaaataggg aaataaacct
     8821 tgaaataagc gacacgaaga gccatcaata cgtaagctgc agcatgggct ttcggaaaca
     8881 tatatttgat ttttgaacaa gaatcgatat accagtcggg cacattattt tctttcatcg
     8941 ccgatagata ggtttctcgc aattcatcag ggatcttgtt ccattgtcct ttacgaacag
     9001 tttccatgat tttaaatgcc atcccgctat ctaatccagc atggatcaaa taaaccataa
     9061 tatcatcacg acagccgatt acttccgcca gtgtcgcatc tcctcgtcga atcaattctt
     9121 ccgcatttcc taaccatacg tctgtaccat gagatagtcc ggaaatttgc agtagttctg
     9181 caaaagttgt aggatgcgtt tcttctaaca tccctcgaac aaaacgagtg ccgaattctg
     9241 gaatacctaa tgtccctgtt ttggaataaa tttgttcctg acttactcca agtacttcag
     9301 gaccggcaaa tatccgcatc acttccgggt catccgtagg gatcgtttgt gggtcgatcc
     9361 ctgataaatc ttgaagcatc cgaataacag ttgggtcatc atgccctaga atatctaatt
     9421 tcaatacatt atcgtgaatc gagtggaaat caaaatgagt cgttttccat tctgaatttt
     9481 gatcgtctgc tggatattgg atcggcgtaa aatcatagac atccatataa tcgggaataa
     9541 cgatgatccc ccctggatgc tggccggttg tccgtttgac tccagtagcg ccttttgcta
     9601 atctgtcgac ttctgcactt cgaaactgca gattattgtc cctttcgtat ccttttacat
     9661 agccatatgc tgttttgtcg gcgactgtac caatcgttcc ggcacggtaa acatagtctt
     9721 cgccaaacaa tactttcgta tagttatggg cttctgcttg ataatcacct gagaagttca
     9781 aatcaatatc gggcactttg tctccatgga atcctaaaaa tgtctcaaat ggaatatcat
     9841 gaccgtcttt gtttaagcgt gcaccacatt ttggacattg tttttctggc atgtcaaatc
     9901 cagaaccata cgtaccatct tcaaaaaact cggaatattg gcactctgga caataataat
     9961 gaggggctaa tggattaact tcagtgattc ctgtcatcgt cgcaacaaag ctagagccaa
    10021 cagagccacg tgaacctact agatagccat cttcattact tttatgcact agtttttggg
    10081 cgatcagata aataaccgag aagccattac cattaataga attcaactct ttttctaatc
    10141 gtttttgaat gatctcaggt aacggatctc cgtacatttg ttttgctttt gtgtaactca
    10201 attctgatat ttcatcttct gaacctggaa tcttcggtgt ataaagttca tctttgactg
    10261 gaatcacttc ttcgcagata tctgcgattt tattcgtatt ttctactacg atctctttcg
    10321 ctgtttcttc ccctaaaaag tgaaatgcag ttagcatctc atcggttgtt cggaaatgga
    10381 catccggcaa actatgacga ttcaacggat tcgctccgcc catagaattg atcaaaattt
    10441 ttcggtaaat ggcatcttct tcgttcagat aatgtacatt acctgttgca acaacgattt
    10501 tatccaaact ttttccgatt tctaccaaat tttggataat ttcttccaaa tcatgttcat
    10561 tttttaccaa ttcttgctcg atcaaaggcg catagaccgc ttttggcata acttcaatat
    10621 aatcatagaa ttttgctcgg tttcttgcct cttctacccc tttttgcatc atggcttcga
    10681 atatttctcc cttatcacaa gctgagccga tcaatagatt ttcacgcatc tttttcaatt
    10741 gggagcgtgg tattcgtggg acgcgttcga agtattcgac attcgacatt gaaatcagtt
    10801 tgaacaaatc tttcagtcct gcctgatttt ttgctaaaat cgttacatgg aaaggtcgag
    10861 cccttttgta agaatctcct tctccgatat gctcatttag ctgatcatga taaagcatgt
    10921 tatgattgtc cattgcctct ttcacaaaga tccatgctaa gtgaccagtt gcttcagcat
    10981 catagattgc tcggtgatgc tgttccaagc taacaccaaa ctttttagat aacacaccta
    11041 atccgaaacg tttgaattga ggatacagat atctagctaa ttccaacgta tcaatgactg
    11101 gattggccgc ttcaggaata ccataccttg cgtagctagt attcaagaac cccatatcaa
    11161 aagccgcgtt gtgggctacc aaaattgtat cttttgaaaa ttcaaggaac atccgcaata
    11221 cttcttcttc cgactttgac cctcgaacca tttcatccgt gataccagtc agatctaccg
    11281 ttgttcgtga caatggatgt cctggatcga taaattcatc aaagctttcg atcacatttc
    11341 ctttatacat tttgactgcg gctagctcga tgatcgtgtc atatactgca gacaatcctg
    11401 tcgtttccac gtcaaatacg acgtatgtgg cttcactcaa tgcttcatgt gcatcattat
    11461 aagcaattgg gacaccatca tctacgacat tcgcttccac gccgtataag attttcacac
    11521 ctgctttttt tccagcacta tgtgcttccg gaaaggcttg cgcaccacca tggtctgtaa
    11581 tggcgatagc tcggtgtccc cattttcctg cttgtgccac aagatcagag atgctgtttg
    11641 ttgcatccat cgtactcata ttgctatgaa catgaagctc tactcgtttt tccccttcag
    11701 gagcataatc tttgcgaggt gtatgtttga cttctatgat atcttgcgca ttcatgacca
    11761 aatcacggac aaacgtatct tcttgaatac ttcctcgtac ctttaaccag ctaccgacac
    11821 tgatcgcatc aaatacttgt tcatcttttt cgccgttcga gaattttttg acgataaatg
    11881 atgaagtata atctgtgatt ttcaaagtca agatcttgcg ttttgaacgt agttctctga
    11941 cttctttgtc gaatacgtac ccctcaagtg tgactcttcg ttcttcttca acgatactga
    12001 tcattggtgt cgttggttca tcggctggaa tatttcgtcc tagctggatg ggtccttcaa
    12061 gagctggcga ttgctctttt ctttcctttt tcttttgttc gtggacaact agactttcag
    12121 ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg ttcttcaaaa agtttcaata
    12181 cacgttccgc ttgctgctca tccacttctg gttcgatacg aaattttggg aatccgtaac
    12241 tgacgaacaa ctcttctact aatggcaagt attgttgctt caaataagaa atagctcctg
    12301 tactgtcgat tggcaagatg actttacgat cttttatcac aggtacctgc gttttcaata
    12361 cttgctgtgc tagtggtgta tcgcattgct gattttccaa agcttgggcc cagtaatctt
    12421 gcaagagctg ttcatcgaat gtctggtcat ctgctaagat attcagtttg acttgtgcaa
    12481 tatctttaaa agcgatagtc agatgctgag tcaatgaccg atatagcata ataggcaaaa
    12541 ttttggaaaa atgtagcgta aattcccata aacgcgattt acgatggacc actacactat
    12601 ccatttcacc agcagtaagc agtggatgat ttttttctgc ttcctctagt tggatttgtt
    12661 ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga tatacgccct ttctgatgag
    12721 aaacaatgca attgatcgca tcttatcctc ttctttttca gtacctttta gtatactcgt
    12781 tttttctgag ttttacatta aaaaattgga actgcgattc aaatcgcagt tccaatttta
    12841 aaagcgaatc atcagattgt caaaaacagc tgattcagct atttttattt tctgtgtttt
    12901 aacgcttatt attcgccttg gttcaagagg attggtaaag tattcgccaa ttcttcttta
    12961 cgtacttcaa ccatttcgcc agttttcttg attttcactt ctacgattcc atccactgct
    13021 tttttaccga cagtgattct gattggacag ccaatcaaat cagaatccgc gaatttcact
    13081 cctgcgcgtt catttctgtc atcaaccagt acttcatagc cagcttttgt catcgcttct
    13141 tctagttcat tcgtcaggct tgtttggtat tcgtctttta gattcatttg gacgatatgc
    13201 agatcaaatg gcgcaattcc ttttggccaa ttgattcctt gttcatctga atgttgttct
    13261 acgattgctg ataataaacg acttacaccg ataccataac agcccataat cacaaatttt
    13321 tctcgtccat tttcgtctag tacagttgct cccatagatt cactgtaacg agtgccgatt
    13381 ttgaatatat gtccgatttc gattcctcga gtaaaggcaa gaacaccgtt gttgtctggt
    13441 gaaggatctc cttcttgtac aaaacgaaga tcttcataag aaagcacagt gaaatcgcgt
    13501 tttggattca catttttata gtggaatcct gtttcgttcg ctccagtaat ggcattcgct
    13561 aaatcttgga cataacggtc tgcatatact tttacgtcat ctgaaacacc gactggtccg
    13621 acagaaccaa actcagcacc tagatatttt tgtgcttctt cttctgttgc ttcttctaaa
    13681 aagtcagcag ataaaaagtt tttcagtttt acgtcattga cttcatggtc tccacgtacc
    13741 aaaacaagga caggttgttc atccgcaatg aacaatacag acttgatgat tttttgaggt
    13801 tcaacagaaa agaaatctgc tacttcgtca attgatttta cgttaggtgt agctactttc
    13861 tccaattcaa gctgtgtttc atgcgatttt tttgatacat agtagcttgt agccatctcc
    13921 aagtttgcag cataatcact ttcagtcgaa taacagatcg tatcctcccc gatttctgag
    13981 atcgccatga attcctttga gtctttcccg cccattgcgc cgccgtctcc gatgatcgag
    14041 cgaaattcta agccacaacg cttgaatact tcggtatagg ctttttcgta atctttatat
    14101 gtctcgtcca aactttcttc cgttgcatgg aaagaatatg catctttcat gataaactct
    14161 cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac gatatttagg ttggatttga
    14221 taaagattta aaggtaattt cttatatgaa ttgatttcat tacgcatcaa atcagtaaaa
    14281 gtttcttcgt gagttggtcc aaggatcatt tttcgatcat tgcggtcttt gaaacgatac
    14341 aagtttggtc catatgtttc gtaacgcccg gattcttccc aaagttctgc tggcaagatg
    14401 gcaggcatca gcatttctac cgctccgatt ttttcaaatt cttcccgcat aatgcgtttc
    14461 agtttttcca atacacgatt tgctaatggc agatatgaat aaatacccgc tgatacttgg
    14521 cggatatatc cagctcttag caagatttga tggcttaata cttctgcatc atttggaact
    14581 tcgcgtaatg ttggaatcaa catttttgat tgtctcattc tatatactcc tttatttcct
    14641 caaaatagtt atcgtttgtt attataccct atttgtcaac tttttctaga aaaagaatcg
    14701 ttgaatatcg ttccatgtaa ctagcaccat caacaacatt aaaaatccaa agccagctaa
    14761 tgtcaatatt ccttcttttt cttgacttaa tggtttcccg cgaatccctt caaaaatatt
    14821 caagactaat ttcccgccat ctaaggcagg aatcggaagc agattgacga ttccgagatt
    14881 cattgaaaga agcgccatca aactaataac agttgttaca ccttgatttg ctgcttctga
    14941 agacaattgg tacatcatga caggtccgcc taatttatct aaactgaaac ctgtaaatag
    15001 tgatcctaag gctttgaaaa tttccaatga accgctgaaa gcttgacgcg tccctccaat
    15061 gatcttatcc ataaaacctg tattcattgg ggcttttata ccaagttggc caactttttc
    15121 gccatttgac tctacagatt tcggtgtcac gtcgacagat tgcacctgac cttctcgttc
    15181 gatcttaaag tctaatgttt tacctggatt tttcgtaatg actgttgtca gatcattcca
    15241 tgaatggatt tcttttccgt ccacactgac tacctcatca ttttctttta agcctgcttc
    15301 tgccgcagct ccattaggca tgatctctcc tacgcgatta gtgttcgtca cttgaacacc
    15361 accttgcata aatgccagaa tgatgaataa cacgattgcc aaaataaagt tattcatggg
    15421 tccagcaaag ttggtcagca tacgctgcca caatttagcc gattggaact gtacgtcttt
    15481 tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc gtcgcatcgt gatcaacagg
    15541 gtaggttact acttctgttt cgtctccatt cacataacca gtgatagtca gctcatcttc
    15601 taaatcataa cggctcaatt ccatcgggat agcatttgtc aattggattt tcttacttaa
    15661 atttatcttc tctacgatgc cgtcagaatt tagtaaaaga gaaagaggca ttcctggtgc
    15721 catttctgtt tcatcatccc cattgcctgc cattcgaaca taaccgccaa taggtaatag
    15781 gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca taaattttcg gacccatacc
    15841 gatagcaaat tcgcggacga ggattcctga tcgtttcgca aagaagaaat gaccaaactc
    15901 atgaacaatc actaatatac caaaaacgat gataaatgtc agaatcgttt tcatagcttt
    15961 tttctccttt acttgttttt tgtgtttttt ataaaacaac gataaaacac tataatttta
    16021 ccatagtata ccaaagacac caatagtatc aatataatga aaaataaaaa cctgcgtctt
    16081 ttatgaaatt cacaaagaaa cgcaggtata cttactaaat aatgccaaat aaatgcatga
    16141 taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat tccaccatgg cctggtaaga
    16201 tatttcccga gtctttcaca tcataatgac gtttgattgc agattcgacc aaatcaccaa
    16261 attgtccgac tgctgaaaaa ataatcgtca tcagaagcat gacaaacata ccatgattga
    16321 atagttcttt tgctgggtat agcaataaat aaagaaatgc gactacaaca gcactaccga
    16381 taccaccgag cgcaccttca atcgttttgt tgggcgaaat atcaggactt aacttatgct
    16441 gaccataacg tcgaccaacc atatatgctc cgatatcagt tgcccaaaca ataaatagac
    16501 caaatatcaa aacagccaag ctttctgttc gtgcattgac aaaattctgg aaacctattc
    16561 ccacatacaa gctgacaagc acaggaaaac ctgcttcatc aatcgtatac gtatttttag
    16621 aaataactga tgttcctaaa agaatcataa ctataagata aaataacata aatgtactta
    16681 cattctctgg aagaaagaaa aaccatcttt cttttggcaa aacaagtaac actgcaccaa
    16741 cggcggataa aactccttca aaacttaaga gtgtcaatcc tttcatccgg aacagctcgt
    16801 aaactcctac taccgctaag attgctgctg taagctcaac cgctatgcct ccgatccata
    16861 taatcggaat aaacaagact agcgccacag ctgctgtgat cacacgttgt ttcatgactg
    16921 atcccctttc tcatcttctg ttttttttac accgccgaat cgacggtctc gattttgata
    16981 agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca ggccaaagcg cttttgtaaa
    17041 atacagctca ctatatgcga tctgccataa cagaaaatta ctaattcgct cttctccact
    17101 tgtacggatc atcagctcag gatcttgtaa ctctttaggc aagaaacctg tcattaaatg
    17161 cttagcaatc aattcatcat cgatttcttc tgcgtgtatt tctccttttg ctacttcttc
    17221 ggcaatttct tggacagcag tcacgatctc tgctctactt ccataattca aagcaaaatt
    17281 caataccatt ccagtattgt tttttgtttg ctcaattgca cgtcttacag catcttgcgt
    17341 atgttctggc aatacgtttt cataacccat gacatgaact ttgacatttt ctttaatcaa
    17401 ttctggtacg aacgtatcaa agaagtcgac aggtagttgc atgagaaaac ttacttcatc
    17461 ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc gtcaacactt tcactccaag
    17521 ccttgatgct ttttttgtga ctttctttac tgtttccatg ccttctttat ggccagcaac
    17581 tcgtggcaaa cgacggtttt gcgcccaacg accattgccg tccatgatga tggcaatgtg
    17641 ctgaggaatt ggtccatcgg tattaaattg aaattcgctt ttttcttgaa tatatttgtt
    17701 tttttgcgga aaaaaacgta acattctttt tcctcctata cttaaaagat tcccactatc
    17761 ttaagtatta tagcaatatc taactgtttt tcctatgaaa tcattgtttt tttatttaaa
    17821 aactgaataa ttttttcttt ttttaatttt taaataaaag aaatataaat aaaaacagag
    17881 gaagcggaat gatccgcccc tctgttttta tgattttatt aaacttccaa taattctttt
    17941 tctttatcag cagcgatctt atctacttct ttaatgctgt tatctgtcaa tgtttgtacg
    18001 tctttttcta atccgcgcaa atcatcttcg gtaatatcac catttttttg ttgtttttta
    18061 tattcatcga tcgcatcacg acggatgtta cgaatcgcga tttttgcatt ttctgcttct
    18121 tttttcacat ctttagctaa ttcttttctg cgttcttctg tcagttgcgg gataactaag
    18181 cgaatgacat ttccatcatt ggttggactg attccgatat cacttgccat aatagctttt
    18241 tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca ttaaaacacg tgcttctgga
    18301 atttggattg atgccatttg gttcaatggt gttggcgcac cgtagtagtt cacagtaata
    18361 cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt gcccaagttc acgttgaaga
    18421 ttttgtgctg cttttttcat tttttctttt gcttctgtca taattgcatc tgccattttt
    18481 atttccccct tacagttgtt ccgatatttt cgcctaagat tgcacggcga atatttccgt
    18541 gttcatttaa attgaagaca agcagtggaa tatcattatc catgcttagt gaacttgcag
    18601 ttgaatccat gacttgtaat cctttggcaa taacttccag atgagtcagt tcttcaaatt
    18661 ttactgcatt tgcatctact ttcggatcag cagaataaac accgtctaca ttgtttttag
    18721 ccatcaggat aacatctgcg ccgatttctg ccgctcgtaa tgctgctgtt gtgtctgtcg
    18781 agaagtacgg gttgccggta ccgcctgcaa agataacgat ccgtcctttt tctaaatgac
    18841 gttctgcttt acggcgaata tatggctctg cgatttgacg catttcaatc gaagtttgca
    18901 cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa agctaaggca ttcataactg
    18961 tagctaacat acccatgtaa tcagcttgag cacgttccat gcccatttgt gctccgattt
    19021 gtccgcgcca gatatttccg ccacccacaa cgattgccat ttcaacgccc agctcgtgaa
    19081 cttctttgat ttcttggatg atttctttga ttactggtgg tttgattcca aatcctgcat
    19141 ccccagctaa agcttctcca ctcaatttta agactacacg ctgatatttc ggtgtgacca
    19201 tgttcattcc tccaatattt tctattgcca ttttaccata cttagatgat ttcgacactc
    19261 taagactggt ttttcttgtt ttgcttaaaa aaagggagcg cacaaaccgt gcgttcccgt
    19321 ctgttattca acagaattat tttttcactt ggctcataac ttcttctaca aagttatctt
    19381 cgcgtttttc gataccttcg ccaacttcga aacgtacgaa tgttttaaca gttgcgcctt
    19441 ttgatgcaac gtatttttca acagtcatat caggatcttt aacgaatggt tggtccacca
    19501 aagcgatttc tgctttgaat ttcttcaagc gtccttctac cattttttca acgatattcg
    19561 ctggtttgcc ttcgttcaat gcttgttctg taagaacagt tttttcatgt tctaattcag
    19621 cttctggaat ttgtgattcg tttacataac gaggattgat agctgctacg tgcatagcta
    19681 cgtctcttgc aactgtttcg tcagttgttc catctagaac agtcaatacg gcaatacgtc
    19741 cgcccatatg caagtatcca ccaaatgcag cattgtcgtc tttttctaca acttcaaagc
    19801 gacggaagct gattttttct ccgataactt gagttgcttc gatcaagtca gattcaattg
    19861 tacctttatc tgttttgatt ttcattgcag cttccatgtc tgctggtttg ttttcagcaa
    19921 caagttctgc aatttctttt actaaatctt ggaacatttc gtttttagaa acaaagtctg
    19981 tttctgagtt tacttcaacg attgctgcag tgttaccttt cacagcaact gaagccaatc
    20041 cttcagctgc aatgcggtca tttttcttcg cagcctttgc cattcctttt tcacgtaaaa
    20101 gatcaactgc tttttccatg tcgccttcta cttcaactaa tgcttttttc gcatccatca
    20161 ttcctacgcc agtcatttcg cgtagttctt ttaccatttt agctgtaacg tctgccatgt
    20221 ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc ttcaaaggag agtgaggctt
    20281 gtcgcccagc ctttgaaaca gctccatcat tcaaaattat tctgctgaag aattgtcgcc
    20341 ttcaacaaca tctacgattt cttcgattga tgttgctgtg ttttcttctg tgaaatcttc
    20401 ttcaactact tgatcttcac cttggtttcc ttcgatgaaa gcatcagcca tttttgaagt
    20461 aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg attactacgt cgatttcatc
    20521 tggatcgcag tttgtatcaa ccatagctac gattgggata tttaatttgt gtgcttcttg
    20581 aacagcaata cgttctttac gtgggtcaac gatgtacatt acatctggga ttcttggcat
    20641 atcagcgata ccgcctaaga atttttcaag acgttcacgt tctttgttca aaccagcaac
    20701 ttctttttta ggtagaactt caaaagttcc atcttcttcc attgcattga tttgtttcaa
    20761 acggctgata cgtttttgga tagtatccca gtttgtcaat gttccgccta accaacgatg
    20821 gttaacgaag tattgtcctg cacgagttgc ttcttctttg attgcttctt gtgcttgttt
    20881 ttttgtacct acgaataatg ctacgccgcc ttcttcagca acgctcttca tgtaatcata
    20941 agctgcatct actaatttaa ctgttttttg caagtcaatg atgtagattc cgtttctttc
    21001 tgtgaagata tatttcttca tttttggatt ccagcgacga gtttggtgac caaagtgtac
    21061 gccggcttct agtaattgtt tcatagaaat tactgccatg tttgtttcct ccgatttggt
    21121 tttatttttc ctcttcttat cttctacctt gccggcaaac tttttcaagc acctaccgac
    21181 aatccaataa gaatgtggat ttgctgatgc ataaagcacc ctcagctatt atacagacaa
    21241 aagatccgta tcgcaagagc aaatatactt ttctgctcaa cattcaatag tttacctgaa
    21301 agataaaaag aattcaagaa aaacagtaga tttctctttg tttctttatt atcctttttc
    21361 cataaaataa ttttaggtat aaaagacgaa tattattggg caatccttct tttttttgtt
    21421 atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg cgcaaagctc aaagacattt
    21481 gctgattaaa caagtcattg aagaattccc gatccgtacg caagaagaac tattaaacaa
    21541 acttgggcat tatggcgtta ccgccacgca ggcaacgatt tctcgagata tccgtgatct
    21601 aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc ttcgtattgt ttcgcgaaaa
    21661 aggaactcct gagactagga ctgaggaaga ggatcggctg gttcaaatga ttgaagacat
    21721 tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc aataccttgc cagacaatgc
    21781 tcatttattt gcatctgttt tagatgagat caaacctccc tctattgtca gtacaattgc
    21841 tggattcgat acgatcatgg tcatctcgga aaatgaggag gacgcaaaac gagtagaaga
    21901 atttcttcac catccaactg aagtatcact tttttaatca gagcacgtat tcctaatatg
    21961 gaatgcgtgt ttttttattt tcaatatttt ccttattaaa taaaccttta cactctttac
    22021 aaaaatacat cattttttat tcaatttttt caacaccttg attgaatcac aataatcatc
    22081 atttatattg gatgttaaca agagcagctt cttgctcttt gtgtatttca aaaaagaaat
    22141 gaaaggaaat catatgtata aattattaac tcctggacca ttgacgactt ctgaaacagt
    22201 gaaaaatgct atgctgatcg atcattgtac ttgggatcaa gaatacaaag aaattacaca
    22261 atggataaga aaaaaattat tggatcttgc tcaagtatcg gaaggaatat acacaacggt
    22321 cttaatgcaa ggtagcggct cctttggggt agaatcggta ttaagttctg tgatcaggcc
    22381 agaagatact gtactgattt gttcaaacgg cgcatatgga caacgaatga tcaacatgtg
    22441 cgaatgtctg aaactgaact ataccgttta tgaggtatcg gaacatcaaa tcctccaagc
    22501 tcatgtgatt acacagctga ttgaagagaa tccctctatc tccgcactag caatggtcca
    22561 tagcgagact acttccggta ttttgaataa tttggaagag attgctgctg tcacacaaga
    22621 aaaagatctt ttgtttatag tggacgcgat gtcaagcttt ggtgggatac cgattcgtgt
    22681 agccgaacta gggattgatt ttttgatttc cagctcaaac aaatgtattc aaggtgttcc
    22741 aggcttttct tttgtacttg ctaataaagc ggaactagaa aagcgaaaaa atgtgagtcg
    22801 aagtttgtca cttgatctat atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg
    22861 gcgtttcact tcccctaccc atacggtttt ggcttttcaa caagcgctga aggaactaga
    22921 ggcagaagga ggaatcgaaa ctcgatattc acgttatctc gccaataatc atgtactacg
    22981 acaaaaaatg acagagatgg ggttccagcc tttcattcaa gaatatcagg ggccattcat
    23041 cacctctttt ctttacccta aggactggcg atttgatttt aatcattttt atcagtattt
    23101 aaagcaaaac ggatttgtga tctatcccgg gaaaatcagc gatcatgaat gttttcgtat
    23161 cggaaatatt ggcgagatct atccggaaga tattttggaa ctgacagcaa ttatcaaaga
    23221 ctataaggag aacgttaatg attaaaactg taatttttga ttgggcagga acaactgtgg
    23281 actttggctg tatggctcct gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc
    23341 aactgactga caaagaaatc cgcgaaccga tgggaaaatt aaaatgggat catatccaac
    23401 agcttctttc cctcccttct gtcaaaaaac aatggatagc tatttatgga caactacctc
    23461 aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt aaagatttgg
    23521 tcaagcattc taccttgaaa cccgacacat tggaaacgat cgaacggttg agaaacgctg
    23581 ggatcaatat tggttccacg actggattta ctgctcctat gatgacaatc gtagcagaaa
    23641 ccgcaaaaaa agaagggtat tcaccagact tcatggcaac ttctgaagac gtcgaagggt
    23701 atggtcgacc gtttccttat atgatccata aaaacatgca gtacttcaaa aacaagtcgg
    23761 ttgatgaagt aatcaaagtc ggagatacgg tctcagatat ccaggaaggg aaaaatgccg
    23821 gagtgttgac agtaggtgta attgaaggta gttcgctaat ggggatttct tatgatgagt
    23881 accaaaaatt agagatgggg aaaaaagatc gtttaaaaca gtctgttgct cttagattcc
    23941 aacaagctgg cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt
    24001 acgctgacaa gaatccagaa tgacttttat acaaaatcag aggtcgtgaa aaagctgtac
    24061 tgcatcaagt aataagaaca gccaaacaaa aatagctcct catatttttc gtttggttag
    24121 gcttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa agggagctgt
    24181 gacacgactt ttgtcacagt ccccttattt acaaacaaac agtgtttaag agacaacctc
    24241 tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac gataaaattt
    24301 gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga tttcacattc
    24361 tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc ttctttcaat
    24421 aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc atcagctaaa
    24481 atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc atgtttcgca
    24541 atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta tttttgaact
    24601 aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg
    24661 acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg cacataaggt
    24721 ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt attcagtcga
    24781 tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc tacagcatct
    24841 ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg gattgcttca
    24901 tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt tttaccacct
    24961 tgcgtgatca aaccaaatgg aatatggcgc atatcgtcag accagtcaaa gcccatttcc
    25021 ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac tacatataga
    25081 gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc acgtgtaatg
    25141 tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata agcagatagg
    25201 tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa aagtttgacg
    25261 atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa acgtacttcc
    25321 agcagatcat agattttatt gaattctttc atcgattcat cacggaacca ttgccataat
    25381 tgaatagcct cttcatcgcc ttcttccaga cgtttgaacc aagcacgagc ttcttcattt
    25441 aattctggtt ctgtttcagc tacttcatgg aattggacat ataaacgaag caattcattg
    25501 attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac gatcagtttt
    25561 ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata accgattttt
    25621 tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc cattgagatt
    25681 ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt cccgatattg
    25741 ctatccccat aatgttcttt ttctttcact actgtttgca aaactttttt gctgataagt
    25801 tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc aaagttggca
    25861 gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg gtaaactttt
    25921 gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg attttctagt
    25981 aattgttcga cttgttccag tgttaaatca tctttgacag cgtcatagac agcttttgcg
    26041 acaagatctt tattattcat ctttattcct cctaaagtgc tcccttgatt gttaaacaaa
    26101 aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat ttcccgcggt
    26161 accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga cgaccgtctg
    26221 tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact
    26281 tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat cttagtctta
    26341 ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt ctgataaaaa
    26401 aacaatccag atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt
    26461 aaatacgaaa gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa
    26521 aagattctcg atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac
    26581 aaatgcaata gctgcctcta ctttcggaag catgcttcct ggagcaaatt gcttttcatc
    26641 aatgtattgt ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg
    26701 gtaattgaca tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat
    26761 ttcggctaat ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc
    26821 agtagctgtt tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg
    26881 atcgaccagc gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac
    26941 tacttttctc catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat
    27001 ttgttctttg gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa
    27061 tgaaggatca ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat
    27121 ccctttttcc ttcagtactt cgcccatggc attttgtaac cagtaaccga tagatccttc
    27181 tgtcattgca acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg
    27241 ctgctgaatc aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc
    27301 tccttgttcg ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc
    27361 ttttgcactt gcatccgtag acaagattgc attaccccct aaagcgacaa ctacttttcg
    27421 tttggccatt tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag
    27481 ccagcgtttt gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta
    27541 tgcttaaacg cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga
    27601 gtgcatacga ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc
    27661 tgttacttcc atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt
    27721 tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt
    27781 ttctaccatt tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt
    27841 atcttcttca cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc
    27901 tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc
    27961 cattttgacg atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc
    28021 tcctaaaatt gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca
    28081 gtaagcaaca gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc
    28141 gatcatttgt gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa
    28201 ttctgctagt tcctctacca ttttttgact gaatccacgg aattcgattc catcaaacat
    28261 acgacctagt actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt
    28321 tgcacctaga tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg
    28381 agtacgtgta gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg
    28441 tgggatacca cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa
    28501 ttcttctttt gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt
    28561 catattaaac attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg
    28621 cagctgacag taagaccgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg
    28681 ttggctcatg cagcgtggac caccacggcc tcgtgacaat tcacttgaat tgatttctaa
    28741 cactttgatg ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac
    28801 tacaacacct ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc
    28861 aacgatctca tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc
    28921 tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc
    28981 gattgtaaag acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt
    29041 atcgtgatct accattgtaa atacggtatc caaatgcatc attgcacggt tgtttggaat
    29101 tttgattgct aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt
    29161 ttctaaagct ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa
    29221 gatcaattcg tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat
    29281 tcctttattt gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg
    29341 acgacgtgct tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg
    29401 gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc
    29461 tgacagatcg tatagactgc tagaacgtac ttcaatatct tttgtacgaa ccccagccat
    29521 gattttatct accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct
    29581 tacagcattc gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac
    29641 attgcctgcg tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc
    29701 attttgcaat gtctttgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat
    29761 atcatcgaac aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg
    29821 ccttttcagt aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc
    29881 catgttgtgt tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca
    29941 ctttcataat tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa
    30001 ataattaaac gtttttctat ttttaagcgt aatttattca ttttttattc tgtatacact
    30061 ttcaaaaaaa tatttattca ttgaaaaaaa ttttttg
//
LOCUS       DAZUKD010000024        29531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000024 DAZUKD010000000
VERSION     DAZUKD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.024"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..910
                     /locus_tag="ACG8N9_000912"
     CDS             41..910
                     /locus_tag="ACG8N9_000912"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4896530.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1075..2346
                     /locus_tag="ACG8N9_000913"
     CDS             1075..2346
                     /locus_tag="ACG8N9_000913"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4896531.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2343..3638
                     /gene="rho"
                     /locus_tag="ACG8N9_000914"
     CDS             2343..3638
                     /gene="rho"
                     /locus_tag="ACG8N9_000914"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4896532.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3737..4000
                     /locus_tag="ACG8N9_000915"
     CDS             3737..4000
                     /locus_tag="ACG8N9_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4896533.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4445..4894
                     /locus_tag="ACG8N9_000916"
     CDS             4445..4894
                     /locus_tag="ACG8N9_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4896534.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4951..6369
                     /locus_tag="ACG8N9_000917"
     CDS             4951..6369
                     /locus_tag="ACG8N9_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4896535.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6369..7229
                     /locus_tag="ACG8N9_000918"
     CDS             6369..7229
                     /locus_tag="ACG8N9_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4896536.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7403..8176
                     /locus_tag="ACG8N9_000919"
     CDS             7403..8176
                     /locus_tag="ACG8N9_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4896537.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8323..8688
                     /locus_tag="ACG8N9_000920"
     CDS             8323..8688
                     /locus_tag="ACG8N9_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4896538.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8836..10044)
                     /locus_tag="ACG8N9_000921"
     CDS             complement(8836..10044)
                     /locus_tag="ACG8N9_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4896539.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10063..10341)
                     /locus_tag="ACG8N9_000922"
     CDS             complement(10063..10341)
                     /locus_tag="ACG8N9_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896540.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10534..11190
                     /locus_tag="ACG8N9_000923"
     CDS             10534..11190
                     /locus_tag="ACG8N9_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4896541.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11632..12429)
                     /locus_tag="ACG8N9_000924"
     CDS             complement(11632..12429)
                     /locus_tag="ACG8N9_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4896542.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12426..13610)
                     /locus_tag="ACG8N9_000925"
     CDS             complement(12426..13610)
                     /locus_tag="ACG8N9_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4896543.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13635..14882)
                     /locus_tag="ACG8N9_000926"
     CDS             complement(13635..14882)
                     /locus_tag="ACG8N9_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4896544.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15097..16842
                     /gene="dnaX"
                     /locus_tag="ACG8N9_000927"
     CDS             15097..16842
                     /gene="dnaX"
                     /locus_tag="ACG8N9_000927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4896545.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16862..17176
                     /locus_tag="ACG8N9_000928"
     CDS             16862..17176
                     /locus_tag="ACG8N9_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4896546.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17201..17797
                     /gene="recR"
                     /locus_tag="ACG8N9_000929"
     CDS             17201..17797
                     /gene="recR"
                     /locus_tag="ACG8N9_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4896547.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17861..18505
                     /gene="tmk"
                     /locus_tag="ACG8N9_000930"
     CDS             17861..18505
                     /gene="tmk"
                     /locus_tag="ACG8N9_000930"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4896548.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18520..18849
                     /locus_tag="ACG8N9_000931"
     CDS             18520..18849
                     /locus_tag="ACG8N9_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4896549.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18863..19801
                     /gene="holB"
                     /locus_tag="ACG8N9_000932"
     CDS             18863..19801
                     /gene="holB"
                     /locus_tag="ACG8N9_000932"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4896550.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19837..20661
                     /locus_tag="ACG8N9_000933"
     CDS             19837..20661
                     /locus_tag="ACG8N9_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4896551.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20654..21001
                     /locus_tag="ACG8N9_000934"
     CDS             20654..21001
                     /locus_tag="ACG8N9_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4896552.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21070..21942
                     /gene="rsmI"
                     /locus_tag="ACG8N9_000935"
     CDS             21070..21942
                     /gene="rsmI"
                     /locus_tag="ACG8N9_000935"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4896553.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22051..23172
                     /gene="dinB"
                     /locus_tag="ACG8N9_000936"
     CDS             22051..23172
                     /gene="dinB"
                     /locus_tag="ACG8N9_000936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4896554.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23226..23828)
                     /gene="nrdG"
                     /locus_tag="ACG8N9_000937"
     CDS             complement(23226..23828)
                     /gene="nrdG"
                     /locus_tag="ACG8N9_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4896555.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24143..26332)
                     /gene="nrdD"
                     /locus_tag="ACG8N9_000938"
     CDS             complement(24143..26332)
                     /gene="nrdD"
                     /locus_tag="ACG8N9_000938"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4896556.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26658..27425
                     /locus_tag="ACG8N9_000939"
     CDS             26658..27425
                     /locus_tag="ACG8N9_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896557.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27415..29457
                     /locus_tag="ACG8N9_000940"
     CDS             27415..29457
                     /locus_tag="ACG8N9_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896558.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLITGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9470 a         5313 c         6180 g         8568 t
ORIGIN      
        1 taacaaattt agttttacaa aacttaggag gaattttatt atgccagtag tatcaggagc
       61 tgaattttta aaagctgctc gtaaaggcgg ttacgctgtc ggcggattca acacaaacaa
      121 cttggaatgg actcaagcaa tccttgaagc ggctgaagca aaacaagcac cagtacttat
      181 ccaaacatca atgggtgcag ctaaatacat gggtggttac aaaatctgta aagatttaat
      241 cactgattta gttgaagcaa tgaacatcac tgttccagtt gctatccact tagaccatgg
      301 tgattacgaa gctgctttgg aatgtatcga attaggctat acttcagtaa tgttcgatgg
      361 ttcacatcta ccatttgaag aaaacttgaa attagctaaa gacgttgttg aaaaagcaca
      421 tgcaaaaggt atctcagttg aatgtgaagt tggttcaatc ggcggagaag aagacggaat
      481 catcggtaac ggcgaattag ctgatattga agaatgcaaa caaatggttg ctacaggtat
      541 cgactactta gcttgcggta tcggtaacat ccatggtcaa tatccagaaa actggaaagg
      601 attagctttt gaccacttac aagcaattgc tgatgctgta ggtccagaca tgcctttagt
      661 attacatggc ggatcaggta ttcctcaaga tcaaatcgaa aaagctatct caatgggtat
      721 ctcaaaagta aatgttaaca ctgaattcca attatcattt gctgcagcta ctcgtaaata
      781 tatcgaagct ggtaaagata aagaaggtaa aggattcgac cctcgtaaat tattagctcc
      841 aggtaaagca gcaatcgttg aagatgctaa atctcatatc gactggttcg gttcagctaa
      901 caaagcgtaa gcatccacat aacagatacg atatatagga atggagaacg acttttgaag
      961 tcgctctcca ttctttttta cttaaaacgg cagaccatgc tcgtttcttt tttttatcta
     1021 tgataaaata caagcatatc catgaaaaaa gattaataaa caaggtggaa acagatgaaa
     1081 aaaatcgtaa tcaaaggaaa ccgaccttta gctggtgaag taacgatcag cggtgcaaaa
     1141 aatagtgcag tcgcattgat tccagctgca atcttagctg attccccagt gatcttggaa
     1201 ggtgtgccgg acatacaaga tgttcattca ttaattgaaa ttttagagat catgggcgca
     1261 actgtccatt tttccaacaa catactagaa atcgatcctc gagaagtcgt atcgattccg
     1321 atgccgcacg gaaaaatcaa tagtttgcgt gcttcttatt attttatggg aacactgctt
     1381 ggtaaatttg gcgaagcggt agtaggcctg ccaggtggct gttatttagg ccctagaccg
     1441 attgacctac acgtcaaagg atttgaagct ctaggtgctg aagtgacaaa tgaacatggt
     1501 gccatgtatt tacggacaga aaataaagga ttgcgtggca atcggatttt catggacgtg
     1561 gtttcagtcg gagcaacgat caatgtgatg ttagctgctg taaaagcaaa aggacagaca
     1621 gtaattgaaa acgctgcacg agaaccggaa atcatagatg tggcaacttt actgaataat
     1681 atgggggcaa aagttcgagg agcgggtaca gatgtgatcc gtatcgaagg agtcgaaaca
     1741 ttacatggct gtcggcattt catgattcct gaccgcatcg aagcaggaac ctatctagca
     1801 ttagcagctg cagtaggaaa tggtatcaaa gtgaaaaatg tgatctttga acatttggaa
     1861 agctttatag caaaattgca ggaaattggt gtccatatga aaatcagcga agatgaaatc
     1921 gaagtttacc cctcgaaaga attaaaaccc gcaaacatca tgacctatcc ttatccagga
     1981 tttgccacag atctgcagca gccacttact gcgttactat tgatgactac aggcacttca
     2041 gaaatcatcg atacgatcta tgcgaaacgt gtgaaccatg tgccggaact agcacgaatg
     2101 ggcgctgata ttaccgtgga aggaaatatg atcatcgtga atggtccaaa taaacttcac
     2161 ggcacagaag tcgtggcttc tgatcttcgg gcaggtgctt gtttagtgat tgctggacta
     2221 ttggcagaag ggacaacgac gatttacaat gtagattata tcttacgcgg ttatgatcat
     2281 attatcgaga aactaacagc cttaggggca tcaattgaaa tgatagagga agtgggagct
     2341 gaatgagcga ttatttgaca atggcagaac tagagaacaa aacgttaaaa gaagtttata
     2401 gttatgccaa agaatttaaa attccttatt acagtaagat gaataaaaag gaattatctc
     2461 tagctgtgat tcgtgcacaa gcagagaaac aaggatttta ttatatggaa ggaatattgg
     2521 atatcgttgg tcaagatggt tacggttttt tacggccaat caattatgga ccaagtgcag
     2581 aagatatcta tatttctgcc tcacaaatca gacgattcag tttgagaaat ggggacaaag
     2641 tggcaggaaa agcccgtcca cctaaagaat cggaacgtta ttatggtttg atgcacgtgg
     2701 aaagcgttaa tggaaaagat ccggaagaag caaaagaacg tccgcatttt ccagccttaa
     2761 cacctcttta tccgcaaaga cagcttcaac tggaaacaac accgcaaaga ctatctaccc
     2821 gaatgatcga tatcttttct ccaatcggtt ttggtcagcg tggattgatc gtcgcaccgc
     2881 caaaagcagg gaaaacttct gtattaaaag aaatcgcaaa tggtatcacc gaaaactatc
     2941 cggaagtaga attaatcgtt ctactgattg atgaacgacc ggaagaagta acggatctag
     3001 aacgaagcgt gaaaggcgac gtggtatctt ctacatttga tctgcagccg caaaaccata
     3061 ctcgagtttc tgaattagta ttggaacgcg caatgcgttt agtcgaagat aaacgagatg
     3121 tcgttatctt gatggatagt attactagac ttgctcgggc ctataactta gtcgttccac
     3181 caagcggacg tacattgagt ggcgggattg acccagccgc attgtataaa cctaagaaat
     3241 ttttcggggc agcaagaaac atcgaagaag gtggcagttt gacgatttta gcgaccgctt
     3301 tagtcgatac aggaagccgt atggatgacg tgatctatga agaattcaaa ggaactggaa
     3361 atctagaatt acaactttca cgtgaattat ctgaacgtcg gatcttccca gccatcgata
     3421 tcaaaaaatc aggtacccga aaagaagaat tattgatgcc aagtgaagaa ttagaagaaa
     3481 tttggaaact tcgaaaaaat atgactggag attcattaga atatacggaa caactgatcc
     3541 gctttttgcg taaaacaaaa aacaatcagc aatttttcaa agaatttcaa gatgtctcct
     3601 ttggtaaaaa aaatccgacc agaaatctga aaagataatt gcaaccttga ctgaaacatg
     3661 ttaagatgtt aatgttgtaa atcgacaaat caactctggt tcagcaaatg gagcagggca
     3721 aaggagcgaa aaaatcatga aagaaaacat ccatccagat taccacccag tagtattcat
     3781 ggattcaaca actggtttca aattcttgtc aggttcaaca aaaacttcac aagaaacagt
     3841 tgaatgggaa gacggtaata catacccagt catccgtgtc gaagtaactt ctgactcaca
     3901 tccattctac actggacgtc aaaaattcac acaagcagac ggacgtgtgg accgtttcaa
     3961 caaaaaatac ggtctcaaag acgcaaacgc tgcggaataa taaaaacgct gataactcaa
     4021 cgtttagcag actatctgat agggatagtc tgttttttta tgttttttgc caatttaaaa
     4081 cttcgtttga tgctgaaaaa gtcgtaggat cataatgcac aaaacaatga aaaactagta
     4141 tatagtttta attatccact tgataaaaac accttatatt aacgaaatga agaggggtta
     4201 tttcgaagta aaaaaccatt tacctaaaat tagaagctaa gctttccttt caaggaaggc
     4261 tttttgtttt gttatagact tgcgattgtt tgtgagctag atcgttcatt ttaatgactt
     4321 atacacgcgg atgatgggtc tttttcctct gttcattcat aatattgaac ggtacagcta
     4381 gcgatgctat actgacctca gttccaagaa aacgttaaca ttttttgtgg gggagaggtt
     4441 ttttatgagg tttgatcaat tgatcgcaga agaactgatt gttttcgatc catcgataga
     4501 ttcaaaagga tcattatttg agcgaatagg ggatgtgttg aaaaaaacaa ataaggtaag
     4561 aaatgttcaa aaagtcgtta aggcactatt caaacgagag gaggagatct ccacaggaat
     4621 agaagatgga tttgggattc cccatgcaaa aagcaaatat gtgacaaaac cagcactgtg
     4681 tttttttcat acagccaaat taacggacta tagcgggata gatgaaatgc caattgaata
     4741 tatttttgcc atcgtagttc ctgaaaaatc agcagatatg catctagaga ttctcagcac
     4801 actctccaga cgattgatga atgcagattt tagaaaggga ataaaagaag ccactacgcc
     4861 agcagatatc atcagacaga ttagtcaaga atgaaaatag tagagtcttt gtccgagtat
     4921 cgatagtgaa gaaaaaggag gaaaataatc atgaaaatcg ttggtgtaac tgcttgcccg
     4981 actggaattg ctcatactta tatgtcggca gaaaaattag aagtaacagc aagaaatcta
     5041 aaccatcaag caaaattcga aacacaaggt gtaaaaacag aaaatcagtt atctgaacaa
     5101 gaaatcaaag aggcagatgc aatcattctt gcagtagata aagaaatcga attggaccga
     5161 tttgccggga aaaaagtaaa gcgtgtctct acaagccgag caatcaaaga accgcaagta
     5221 gtaatagacg aagcattgag agatattggc gtattcgttg tgtctaatga aaaagaacca
     5281 gctgcaaatg agaaaaagcc gacaatctat aatcatttca tgaatggtgt gaattacatg
     5341 cttccatttg tgatcgcagg cggaatcatc atcgcactga gttttgcctt tgggattaca
     5401 gcggctgatc ccaactcagc agactataat gtgttagcag ctgcattttc aaggatcgga
     5461 ggagatacag cgtttgccat gatggttcct gcgttaggcg cggggattgc tacatcgatt
     5521 gctggaaaag caggttttgc cccaggaatt gttgccggat tactggcctc tacaggtggt
     5581 tcaggttttc ttggcggaat gattggtggc gtgctagcag ggtatatctg tgattttctg
     5641 gcaaataaaa ttagcgtaaa aaaagaattt tctgctattt atcagctgat tgtcgtcccc
     5701 tttttatcga tcttaattat tgggcttttg atggtatttg tgattgaaca accgatcaca
     5761 tgggtgttgg atgctttgac aaattggctg aatagcttgg gaaatacatc cgggctattg
     5821 ttcggattac ttatcgggat catgatggca gctgatatgg gtgggccgat caataaatcg
     5881 acttcgactt tctcgatcgg tttgatgtca gctggagtaa atgcaccgat tgctgcttgt
     5941 atggctgcag gaatgacacc tcccctaggt attgctttag ccactttatt attcaaacat
     6001 aaatttacca aagaagaacg gacttcaggt caatcatgct ggattttagg cgcttcgtat
     6061 attacagaag gtgctatacc gtttgcggta tctgacccga tccgcgtgat acctagtttg
     6121 atgacaggtt cagcagtcgc tgcagggatt tccatggcag taggagttac ttcaatggca
     6181 cctcatggag ggatttgggt gatgctaatt ccaaatgtta tgaacaattt acctatgtat
     6241 gtcttggcaa ttgcatcagg tacagtcact acagccttta tgcttggact attgaaaaaa
     6301 acagtgaaag agaaaaaaca agacaagtgg acgaaaaatg aaaatctaac aaatgaggtg
     6361 gaagcgtaat gttatataca atgaaagagt tattgaaagt ggctaaagag aatcaatttg
     6421 cagtaccagc cttcaatatt tgcagctatg atatgatgaa agcaatcatc gaagaagcag
     6481 agagattaga agcaccagta atcttagaaa ttcatcctga tgaaatcgcc tatttaagtg
     6541 atgagtttgt tgcggcagta agagcctatg cttatacaag taaactgccg atcgttatcc
     6601 acttggatca tggcggtacg atagaagatg tcttacgggc gattcgaaac ggctattctt
     6661 ccgtgatgat cgatgcgtct ttgaaatcat tcgacgacaa tgtacagatt acgaaaaaag
     6721 tggttgcgtt agctcatgcg gtaaatgtct cagtggaggc tgagcttgga acgattggga
     6781 ataatggttc tgctgaagga ggtgctgaaa caatcattta tacagatcct gatcaagcga
     6841 agatttttgt gaaagaaaca ggaatcgata cacttgctgt agcaattgga acagcgcatg
     6901 gcttgtatcc gaaaaacaga actccaaaat taaatatcga cttactgaaa gaattgaacc
     6961 aacaaatcga tcttccattt gttttacatg gcggttcagg taatccggac gcagaagtca
     7021 gcgaagcagt aaaatacgga gttgccaaag tgaatttgag ctcagatctt aaaagcgtct
     7081 ttttcaatgc tttgagagaa atattgagtg aaaatccatc gatgtacgag ccgaatgcaa
     7141 tttatccagc agctaataaa aaggtacaag aagtggtcaa acataaaatg gaaattttga
     7201 acacagtagg caaggctagt ttatactaaa aataaaaatg tgtggtcaag agggtatgac
     7261 aatagtcttg tcataccctc ttaaccacac acgatgttca gaagtagttt tttcgaaaaa
     7321 tttcttctta tttctcgaag ccaatcactt ctgtcacaac ctcttttttc ctgttactat
     7381 ataaagtaaa tggggtgatt ttatgttgaa tgaagtggag aatagacagc aggagattgt
     7441 gaattatcta aaaaacaacc aatttgcaag agtcaccgat ttgatcgagt tagtaaatta
     7501 tagcgaagcc acgattaaac gtgaccttgt ttttctggaa aaaagtggat tgatccgtag
     7561 agttcgcggt ggcgccatgc ttattgacaa tcgaaaaatt gacgtgcctt acctgatgaa
     7621 gatcaccaaa ttagaagaag aaacagaaaa acgtaagatt gcagatacag cttcttcttt
     7681 gattcgagat gatatggtct tattcttgga ttccagtaca acctctttac acttagtcag
     7741 aaacctagca agatttgatg gtttgcagat cattacgaat ggcatcgtca cagctgctat
     7801 gttgagtgaa tatacaacgg caaaagtatc tgtattggga ggggcaattg ttccgaaacg
     7861 agcaacaatc aacggttcaa aagcctataa cgatgcattg tcatattatg cggatttagc
     7921 gataatgagt tgtcgcgggc tagatttcag ccaaggagta acagaaactc atgaaggaga
     7981 ggcaatggtc aaaaaagctt ttagaaaaca atcagaccgg cttgccgttt tagttacaca
     8041 gcaaaaaatt gcacataaat tcatgcatca agccattgct tttcatgaaa tcgattatct
     8101 ggtgacaagt aaagaactga caaaagaaca gtatgcgaaa gtaaaggaac accatatcga
     8161 atgtctgtac ggataaatta ttacgtgaat gttctaagtg gcttcctcat ttttagatag
     8221 gaccttatct ttgctcgctt ctctcagccg atggtataat aatgaaaagg ttatcttgtt
     8281 agataatctt acttttttat ccaggagaaa ggagtgcaga aaatgaaaca tgtgaaggcg
     8341 ttggtagtga aggcaatcat gatctgggcg attctgtggg tcgtgctgac aggtttatac
     8401 ggtgttagct ttatggattc aacgattgtt ggggtaatta ttgtagtcat gatctatgtg
     8461 ctaggggatc tgttgatctt acgtaaagta gggaacatcg cagcgacgat cgcagatgct
     8521 ggttcagctg cagttatctt gtggctctat ctatatttta tgaatgatac agtcgatatt
     8581 tggatgaaag tattgattcc tgctctattg atcggtgtgg cagaatggtt cttccacaag
     8641 tggttgctaa gtcaaggcat cgtaccagat gaacggaaaa tggaataaat caacagttaa
     8701 actattctta tcgataaatg aaagctttaa aaataaatag atgtttaatt aagaataatc
     8761 ataaactatt tttactcaaa aaaccgctca gattattttc agatctgaac ggtttttttt
     8821 atctattcat caagatcaaa tttcattttt caatgattca atcaattcgc cctctgcttc
     8881 atgactatgt tttcggccat taacaagata gagtgctgta catatcaatg gaatgactcc
     8941 ggcaatcaca tacaatcctc taaatgaagt aagagatttt agcatcccca tcaaataagg
     9001 accaacgcct aagcctaggt ctaaaccaat aaagtaggta gataaagcaa tactgattcg
     9061 atgttcgcgg acgattttta aacaaacagc ctgtccattt gacataaacg taccataacc
     9121 taggccgact aatgctccag agataagcaa tgtcacacta ttcgtcgtta cactcagtaa
     9181 aaataatcct gctgttaaga ataaatagct aggatacatc acgtattgtt ctcctttgac
     9241 atcgaaaatc cttccagtca atggacgtgt aaatgtgatg accaaagcat atacgacgaa
     9301 gaaaaatgac ccagcagcaa ctaagtggat tacttttgta taagaagata aaaatgataa
     9361 aacacttgaa taagaaagac ccatcaaaaa ggcaattcct gtaatgaaaa gtgctttctt
     9421 ttcaataaaa ctattgatat tccatgagcg aagagatatt ttttgttctt cagataatgg
     9481 aatatttttt acaggaaaga caaggcaggc aatcgtaaca aagaagacca gtacaattga
     9541 aaaccagata atgaaacgaa aatcagtcat attcagcaaa atcattccta aaaatggccc
     9601 aacagcggca gctaaactag tacttaatcc ataataattg atgccttccc cttttttaga
     9661 tgcagggata tatgcagtaa cgatagcatt tgtagctgta gaaactgtac cgtagccgaa
     9721 tccattcaag aatcgaacta aatacaatgc cccgatagtt ggcgtataaa gatatgctaa
     9781 tgttgaaagg agatagaaga tcgcgccgcc tcttaaggta aacttccgac caaatagttc
     9841 taactgtttc cccatgaata agcgtgccag caatgttccg atgatataga tcccagaagc
     9901 aaaacctgct tggctcaagc tggcgtgtaa ggtgtcttgc gcaatgactg ccatgatgac
     9961 catcaacaga taataaatca ggtagacaat aaaattgatc aatgtgattc caataaaata
    10021 tttattaaat aatttctttt ccatgtatgt tatacattcc ccttaagctg aatatgcttt
    10081 taatttttgt atttgaactt ctacatcgtg ataattggat ggatagcgat caacaagttg
    10141 gcgaataaat aaatctcttg tagacataaa cagatttaaa aattcttcgc attcgtttgt
    10201 tggattctca tccactgctt tgatcaattc gatcgagttt tgatacgcat catttaaaaa
    10261 gactaaagaa gctttcatat caaatcttgt ttgatatgct gtttctaatc gattaatgat
    10321 tgtgttcatc cagtaattca tgttttttcc ctctattctt aaaaaatttt acttctgcag
    10381 tataacgaat aaaaaaaagg aaaaaaatcg cataagtgat ggaaaaaggt acttaacaaa
    10441 tgttaatatc attagtaaaa tacctttgga ctttaaaaag ttttaaagta ctgtatgatc
    10501 tttttagatg aaagtttgga tgggggagca tcattggata gtagagtctt aaaggaatat
    10561 ttggataaaa atgattttcc gattatacgc aaaggatatc ataaatattt gacattcgaa
    10621 gggatggaag attcccatac gtatatcttg aaagagggaa cggtaaaagc aagtgttatt
    10681 tcaaatgatg ggagagaatt taaccttcgc tatatcaaag atcttgaaat cgtctctctt
    10741 ttaaaagacg aatattcaca attcatcgat tcgccgtata atattcgagt tgaatcagaa
    10801 tatgcagagt tttatcgaat caatcgtgtg cgcttttggc aaaatatcaa ccaagaccca
    10861 aagctacaac actatgtaaa agagtattac cgctatcgtc tgctttattc gatgaaaaaa
    10921 atgcagcaaa tgctattaaa cggaaagctg ggagctgttt gtacgcaact ttacgaatta
    10981 tatgatttat tcggtgtgcg gacagaagaa gggatgctga tcgatttttc gatcacgaat
    11041 gaagaaatcg ccaaattttg tggcatctca actgctagca gtgtaaacag aattttaaaa
    11101 caactgaaaa aagatggtat tatcaccgtt aaagaccatc atatcgttat tttgaatatg
    11161 gacttgttag aagacaatat tatcttttga tgaacaaaga ggttgtgaaa tcagcgtttt
    11221 gacctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc gttttttgag
    11281 ctaataccga gggtcagctg attgaacacc gtttaccaag aggttgtgag cctgccgttt
    11341 aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg
    11401 acttgtgacc gaggagttgg taggagaaca ccgtttacca agaggttgtg aaaaagctgt
    11461 tctgcatcaa gtaataagaa gagccaagta aaaacagtct cttatgtttt taacttggct
    11521 ggacttatca ccgtagatgt aagcttttga acgccgttta tttacaacaa aaggggctgt
    11581 gacacgattt ttgtcacagc cctttttttg cgaacattca tttagcaatt tttagaaact
    11641 tctgccgcca ccgccaaaag tcccgccacc gctagaatgg gtagtacttc cgccaccgcc
    11701 gcttccagga ggtgggcttt tggggattct tcttgtcgtg acaaaagaat ttgtcagacg
    11761 atcggttttt tcagttaatt tgatactgga tttttctcgg aatggatatt tgtagatgcc
    11821 tgatttcaat tgataacgcg aaacagtcac aaaataaaag acaagactaa ggataagagc
    11881 caaaacaaca gcaatgatga tttctactgt cgtcaaaacc ttatagcgtg taattttgcc
    11941 agtttcttcg tctacacgat aatgtcctcc tggaactccg tcttccacat atgttgctga
    12001 tttagacagg taagcttttg cagcattgta ataggcagca tttgtcatct gatcataaac
    12061 atcatctaag atcgaatcga ttctgctatc agtcaaataa tcgatcatat taccagaagt
    12121 cgaaatgtag atttcacgct gtcccatatc taatagcaat actgagccat tttgatcatt
    12181 tccgactgcg tctcttaagt aattatcagc aaagtctctt ggttcttctg aattagatgt
    12241 agtcgttacg atcaagactc gtcctttgat tttttcattc agcttttctg cctgttgttc
    12301 caaagattgg atttgatctg ttgtgaataa cccagcctca tcatttaccg tagctgtatc
    12361 tgcatgagta gccaccggcc agccaacact tactagcaaa agaaagaata aaaaaaattt
    12421 tttcatcata tcaagtaacc tccgatcaga aagaggatcg ctagcccagc gaaaatccca
    12481 agagcggtca gtcccaattt tttctggctg atcggcagga caccgctgac ctttcctgtt
    12541 tgtccattca ttgcgtagta gtagactttt ttacttgagt cattgcttcg ataagtaaca
    12601 agccataccg gcagtaatac gtaattattt acttcgtttg tgattgaagc agaagttcgc
    12661 acaccggtca atgttgtata tccatttgcg gtatctttta acagcttctc tgaatagtct
    12721 tttaattcag attgaatctg tgatttgatt gcctcatatt cgatatctct tttttctgct
    12781 tggaacccgg ctaaatactg gcttttaaaa tcaattgctt tatctaacgg gaaaggttgc
    12841 acgccttcga ccatcttttg ttgcgtattt tttgataagg cattttttac tagttcttta
    12901 aaagaaagct tgcctgctct tgaaacagca aattgcttcg tttctgtgta ttcgatgtca
    12961 cctatccgcc atatgcggat aactgtacca ttcgcctgca tggcaccatc tacttcagca
    13021 tctgtagccc aataagggaa atacacacct gtcaacttat cgatttgatc cttattgaaa
    13081 aaagctttgg gaacaaacca ttttttcttg gtccaagcga gaaatctgtt gattgcttct
    13141 tctttctcga cagcaaaagg aagtacttta tttggcaaga attctccgct taaacgtccc
    13201 gatagtacga cgggattatg gcagtagtaa caataagtcg cagcagttgt cgcatcagtc
    13261 acgatctctg caccacagtt gggacaaaga aaaagatcta tcgctgcggt attatctgca
    13321 gattcgtccg ctgtatctga aaaaccgcct gcctcgtcaa ccgctttgcg ctcggcttca
    13381 ttcatatcgg caagctgttc ttgggctgta aaagtaaaat cttcggtagg tgaaggttct
    13441 tctttgcttt cttcgccagc catacgcgct tctttttgct tttgctcata ttggctgact
    13501 tcttcttctg tatagatatt caggcagtat tcgcaatgaa atttttggtc tttgggatca
    13561 aaagtcaacg gaccgccgca attaggacat ttatgtgtaa gaacatccat cagctgcaca
    13621 cctttctaaa taaattaatt aacggggatt cctttaaacg gtttaccaca gtttggacag
    13681 aatttaggga tgccgttgga aagatccacg atctcattgc attcactgca tcgcatttgt
    13741 agcttaggct gaacgttttc tacaggtttg gcagctccgc agttggaaca gaattttccg
    13801 gaattttctg tcccgcaaac tggacatgtc caagtatccg catttccttg tgcagtttgt
    13861 tgattttgtt tttctgcttg ttgtttgatt tgttcttggt tattttgagc tgcttgagaa
    13921 agataagaac catttgcatt cattcccata cctacaccca taaagccggt cattgctcct
    13981 gcttcatttt ttccggctgc ttccatacca cgtgcaatgg aaccttgcac gtatccttca
    14041 cgtacgctag gatcacctag catcgcccct tcgttacgca tattgatcag tttcacagaa
    14101 tcatcggtgt aagaaatgct ggctacagcc acagaaacaa tctccatccc acgtaattca
    14161 cgccaagaat catccaatgc agtatccatg tatttgctta attccaaact cttagatggt
    14221 acataagaaa tccgttgacc atctgcagac atctgattga ttgctgattg tagcgctgtt
    14281 aggaattcag ctaaatactg ctcattgata tcattgattt ccacttgtgt tttgttttta
    14341 gggattgcgt ttgtataaaa taaaatcggg tcaacgacat gaatagaata agtgccatgt
    14401 gcccgtaaga ataattctgc gttatagaaa ttgtcaaagt agttcaacgg cgcgcttgta
    14461 ccgaatttga ttccctttat ttcttggaga ttgatataaa aaacttgttg tttctgcggt
    14521 gtgacgccgc caaatttgaa tcggtcaaaa gtttcagcaa ttgcttcttt cagtgttcca
    14581 ttgaacatgg aaggagctgc gtcattttta atggtgtaat aaccttcctc agcggtataa
    14641 tcgatgattt ttccaccatc gaccaaaagc atcatcatgt ttggatagac atggacgact
    14701 gttccgtctg ttaaaacatc ttctgttcct ttacgattgg agccacgttt gtcatctttg
    14761 cggactttga caccttttgt catgaccgtt gtatcactca tattgtctgg ttcgatgact
    14821 tctagccatt gatctgccag accgccgcca accatacttg ttgctgcttt gataagaccc
    14881 ataaaaaatt cctcctctga ttcagctagt gccaaacaat tattgtttat ttattatacc
    14941 ataggaagat gtgaaacttc tcggactaga gacggaattg aaatggaaaa ttgcgtattg
    15001 tttaaatacc tgaaaaagcc gagaaggata gtagtttttt tacctaatat cacgtataat
    15061 aagagatagt gaatttttta aaaaaggggg atccttatgg catatcaagc actttatcga
    15121 gtctggcgtt cgcagcgttt cgaagacatc gttggacaaa aagctgtgac acagacacta
    15181 aaaaatgcta ttgtttccca caaaacctca catgcctact tgtttacagg acccagagga
    15241 accggaaaga cgagtgcagc aaagattttc gcaaaagcaa tcaactgtcc gaacagtaaa
    15301 gacggtgagc catgcaacga atgcgaaatg tgtcgatcaa ttactgctgg tacgcaagaa
    15361 gatgtgatcg aaatcgatgc tgccagcaat aacggagtcg aagaaatccg atttatccgt
    15421 gatcgggcaa attatgcgcc tacaaaggca gcgtacaaag tgtacattat tgatgaggta
    15481 catatgcttt caacaggagc tttcaatgcc ttgttgaaaa cattggaaga gccaaaagaa
    15541 agtgtcatat tcatcttagc aacgaccgaa cctcacaaaa taccagctac gatcatttca
    15601 cggactcagc gatttgattt taaacggatc aatacggcag atattgtcga acatcttgaa
    15661 tttatcttga aagaatcgac tatcagctat gaaccagctg cattgaatgt gattgcccgt
    15721 tctgcagaag gcgggatgcg ggatgcattg agtattcttg atcaggcaat ctcgtttagt
    15781 gaaggaacaa ttactttaga tgatgccatg caagtgacag gtagcttaac tgacgaaatg
    15841 atggacagtt acttgagtgc ttgcgtgaaa aaagaagtac cagaagcatt ggaaacattg
    15901 aaccaaattc tcgcagccgg caaagaatca cgtcgctttc tagaggatat gttacaatac
    15961 tgccgtgact tgctgatgta tcagcaagca cctaacctag ttgaggaaca atcgagtacg
    16021 atcagaagta catttacaga attggcacaa actgtttcag cggatcagct gttttcaatg
    16081 ataaagattt tgagcgatac tcaaaatgaa attcgtttta ccaatagtgc cgctatttat
    16141 ttggaagtcg ctacagtcaa acttgcacag ctaccgtctg cttctgttcc ttcagcagat
    16201 gtagcatttg cagatgatca gatagtaaaa cagctacaac aagagattaa ggaactaaga
    16261 aacgaaatca aggaactacg cagtaataca ggaattgaga atacaaaagt acagccggta
    16321 cagcaaacaa atcataaaaa acagacaagt acctatcgtg taccgacgga acgagtatat
    16381 aaagttctgc gggaagcgac aagacagcat ctgaaccaag taaaagaagt atgggaagac
    16441 ttgctgatga gtctatctgt cactcagcga gcaatgctta aagcaagcga accagttgca
    16501 gctggaccta caggactagt gattgcgttt gattatgaga ttgtttgtca aagagcagca
    16561 aatgacgaag aattgcagct ttcggtacac aacaacttaa gccgaatgat caaagattat
    16621 gcgcctgatc ctgtttatat tacaagagaa agctggccag aacttcgaag aaattatttg
    16681 acgcaggcac aagaagaggg aacagaaaac tcttttgctt ctgaagactc agaaggagaa
    16741 atcgagttgc ttcctcaaga acaagaagta ccagaagaaa cacttgtagt aaaagcagag
    16801 gaactgtttg gctccgaagc tgttacagtg gtagatgatt aataaaaagg agtagataat
    16861 tatgatgcgt ggaatgggca atatgcaagg catgatgaaa caagtacaaa aaatgcaaaa
    16921 agaaatggca gaagcacaag agctgctaaa taaaacagag tttacaggta aagccaccaa
    16981 tgaattggtc aaagcggttt ttacaggcga tcgtcgcatg aaagatatcc aaatcaatga
    17041 agaagtcgtt gatcctgatg atatcgagat gcttcaggat cttgtcgtaa tggcagtcaa
    17101 tgatgcgatc gcacaaattg atgtggaaac tgagaaaaaa ttagggaaat acgcaaaagg
    17161 tttgccagga ttttagtttt aaactgaagg agtaatgaat atgcaatatc cagaaccaat
    17221 tgcgaaactg atcgacagct acatgaaact tccaggaatc ggtcagaaaa cagcgacaag
    17281 actcgccttt tatacgatcg atatgaaaga tgaagtagta aatgaatttg ctaaatcttt
    17341 gttaagtgta aaacgcgatt tgcatttttg tagtatctgc ggaaacatca cagaagaaga
    17401 tccctgcgaa atatgtagaa atccttcacg tgatcgaagc gttatcctag tggtcgaaga
    17461 gccgaaagat gtaatggcaa tggaaaaaat gcgagaatat cacggacttt atcatgttct
    17521 ccatggtgtg ttatcaccga tggaagggac aggtccagaa gacatcaata tttcttctct
    17581 gttgcagcgg ctgcatgatg cagatgtaaa agaagtgatt atcgcaacaa atgcaacaac
    17641 agagggagaa gcaacagcta tgtatctctc acgtctgatc aaacccgcag gtatcaaagt
    17701 gactcgtcta gcacatggtc tgtctgtagg ctctgatatc gagtatgcag atgaagttac
    17761 tttattgaaa gctgtcgaag gaagacgtga gttataaatt tctaattaag tgttttttca
    17821 gatacaatat aaaagcagca caagagatgg gggcaacaat gtgaacggac tatttattac
    17881 aatcgagggg cctgatggtg caggaaaaac cagcatcttg aatgaacttt accctctttt
    17941 gaaagaagta gcaaaaacag aaatcattca aacaagagaa ccaggaggca ttccgattgc
    18001 tgaagagatc cgagcagtga tccttgatcc aaaaaatgat cggatggatg aacgtacgga
    18061 agcactgctt tatgcagcag caagaagaca acatctagtc gaaaaggttc tgcctgctct
    18121 ggcaaaaggg aaaatcgttt tatgcgatag atttgtcgac agttctcttg cttatcaagg
    18181 tgcaggccga cgcatcggcg ttaccgagat tgctcggttg aatgagtttg ctacagaagg
    18241 tactacgccg gattttaccc tttatcttga cgtagattca gacaccggat taagaagaat
    18301 caagaaaaac agacaaaatc aaatcgaccg tttagattcg gaaggtttgg aattccacca
    18361 gcgtgtacgt catgcttact taaagctggc agaagagaat ccggaaagaa tccacaaagt
    18421 tgatgcaaga aaaagctttg aagaagtgct gcaaacaagt tatcatacaa ttattgaaca
    18481 gtatccgcaa ttttttgaaa actaggaagg tgaacgttta tgaaaattat tttagcgatt
    18541 atccaagaca aagacagcaa ccgtttatca aatgaattga ttgatgcaaa tatccgtgcc
    18601 acaaaactat catcgacagg cggcttcctt aaggctggaa acagtacgtt tatcgtgggt
    18661 attgaagatg accgtgtaga agaagcattg gaaatcatca aaaaaacgtg tgaatcaaga
    18721 aagcaatttg tatctacacc tgttacactg gatatttcga tggatggggg cgtcccttat
    18781 ccagtagaag tagaagtagg cggtgccaca gtcttcgtct tacctgttga aggattccat
    18841 caattttagg aggagctgag ctatgaatca agaaataaac ttggctcaaa tgcaaccgat
    18901 cgtgtatcag cagcttcaac ggagttttga gcatgggcgt atcgctcatg cttatctttt
    18961 tgaaggggag aaaggaacag gaaaacatga agtaggtata tggcttgcac agcacctgtt
    19021 ctgtacccaa atgaaagaca atctgccttg tggaaaatgc aacaattgtc agcggatcga
    19081 aaagaaagaa catccagatg ttttggttat tgaaccggaa ggacaaacca tcaaagtcga
    19141 tcagatcaga cgtttgcaga cggaattcag tcgcagcggt tacgaatcaa gaaaaaaagt
    19201 ttttttgatc aaagaagctg aaaaaatgaa tagcagtgcg gcaaacagtt tgttgaaatt
    19261 cttggaagag ccccctggag attttttagc ggtgttagaa acagatgcag taggaagaat
    19321 tctccctact attcaatctc gttgtcaagt tctgcatttt caagaacttt cgaaagaagc
    19381 actgatccat cgattgcaag aagagcagat tccattggaa aaagcaaaac ttttggcttt
    19441 cttaacaaac agtttaggaa aagcagttga aatctctcaa gatgaatggt ttaatgatgc
    19501 taaggacttg atttatcaat ggttcgttta cttgcaaaag caagatactc aagcctttat
    19561 ctatgtccaa aaaaaattgg taaaatcatt taaagaaaaa acacaacagt ttacaggatt
    19621 atctgtcttg atgttttatt atcaggaagc acagcgtcaa gcagtagcaa acggcgaata
    19681 tggcaaagtg aaacagataa accagataat tgaacggata ttgatcgcag agcagaaact
    19741 acgcagcaat gtcagttttc aggctgttgc ggaacagttc gttttgcata cgatttttta
    19801 gcttgccgca aaggatgatg atcaaggagg atggaaatgg tagaagtagt aggtgtccga
    19861 tatagagatg caggtcatat ctattatttt gcaccaggag actctgaata tgtttatagt
    19921 gacaaggtat tagtcgaatc tcagcaatcc ttacatattg ctactgttgt ccttcctaaa
    19981 aaagagattg atcccaatga ccttcctgaa gctatccgtc cgatcttgca taaagcgtca
    20041 caagctgaat tagaaaaaga caaaaaaaac aaaaaagatg cttatgatgc attgaaaatc
    20101 gcaaaagaaa aagtgcagtt gcgtgagttg gaaatgaaaa tggtcaaagc cgaatacaca
    20161 tttgatcgaa ataaattgat tttttctttc acagcggacg ggcgaattga ctttagagaa
    20221 ttggtaaaag atctggccgc tgtatttcgt actcgtattg agcttcgaca aattggtgtc
    20281 cgcgatgaag caaagataat aggcggaatt ggtccatgtg ggcgtccatt atgctgttct
    20341 tcttttttag gcgatttcgt gcctgtatca atcaaaatgg caaaagatca aagtttatct
    20401 ttgaatccaa caaaaatttc cggtttatgc ggacgcttga tgtgctgtct gcaatatgag
    20461 agcgaagaat acgagacagc gaaaaaagaa ctccctgact ttggaaaagt aattgctaca
    20521 cctgatggta aagggaaagt cgtagggttg aatctgctta gccgtatcgt tagtattcgg
    20581 ctgactggac gggaaaatcc tgtcgaatat gaatgggatg aattgaaaga atttatccaa
    20641 gtaggtgaga tgaatggata agagatcatt atacgacgga ttgaatcaat tagaatcaga
    20701 gttgagaaat acattaacag aacttgttga aatgaagcat gctttgcatg agatcgtaga
    20761 aaaaaataca actttggaga tggaaaatca acgtctgcgg gaacacctgc gggaattgaa
    20821 ccaagttgca caagctccag ctgaaaatgt gaagcaggaa ctttctaagt ctcgtatgaa
    20881 tcttgaaaaa atttatgaag aaggattcca tgtctgctac gatttatatg gatcaagaag
    20941 ggacaatgac gagccttgtg cgttttgtct agaagtgatc tatcgcgaaa gcggtcgatg
    21001 accaataaca agaggagact ggagcggaga aatactgctc cagtcttttt attaaggaga
    21061 ttgtgacaga tgtataatca aaaaagtttc aagggaaatc cggtatatgg ggatttatat
    21121 cttgtaccga cgccaattgg aaatttagaa gatatgagct acagaagtgt tcgtatgctg
    21181 caagaatccg atcttatcgc aagtgaggat acgcgtaata cacaaaagtt attgaatcat
    21241 tttgaaatcc atacacccca aaaaagttta catgaacata attataaaga acgtgtccct
    21301 caattgattg ctttgttaaa agaaggaaaa acgattgctc aagtcagtga cgcaggaatg
    21361 ccttccatca gcgatcctgg acatgagcta gttttagcct gcataaaaga agggatttct
    21421 gtgatctcta tcccaggacc tacagctggg atgacggctt tgattgcttc agggctagtg
    21481 cctcaaccat ttttatttta tggcttcctt ccacgtaaga aaaaagaaca gaaagaaaca
    21541 ttgaaaaagc taaaagaaca gacagttacg ctgatttttt atgagtcacc ttatcggatc
    21601 actgctacat tgacgaattt cttagaaatt tttggaaatc gccaagtcgt tttgtgtcgg
    21661 gaattgacaa aggtccatga agaatacctt agaggaagta cggaagagct actcgcttat
    21721 atcgaagaac atccagtaaa gggtgaatgt tgtctgcttg tagaagggaa tttgttcgaa
    21781 atgccagtga cggaagttga tgaagagttg ggaacgttaa aggaacaagt agaagcaaag
    21841 atcgcttcag gcgaaaaacc aaatgctgct atcaaagcag tagcgctgaa aaatgggctg
    21901 aaaaaacaag aagtctaccg tcagtaccat gaattagact gagatagtat aagttgtcta
    21961 taatgaaaga ctaatgagga aatcagctac taacttttca aatctcttga acgcgaacga
    22021 atgttcttgt ataatgaata agaggtgagg atgatggagc tgcaatttcc tttacataat
    22081 gatacatcaa gaaaaatcat tcatattgat atggatgctt ttttcgcttc ggttgaagaa
    22141 cgagatcatc ctgagtttcg cggacatcca ttagtaattg ccagacaccc cagtgacact
    22201 ggtgggcgtg gagtagtgac tacagcaaat tatgaagcaa gaaaattcgg aattcattca
    22261 gcaatgagtg cacaaaaagc gtatgagctt tgtccgcaag ccatctttaa gcctggcgac
    22321 catcataaat acagcgatat ttccaaacaa gtacgggaag tattctatcg ttatacagat
    22381 attgtggaac cagttagtat cgatgaagcg tatcttgatg tgaccgaaaa taaaatcaat
    22441 tgcaagtcag ccatcaaaat cgcccgaatg atccaagcag atatttggga gagtgtccag
    22501 ctaacatgtt ctgcgggagt aagttacaat aagtttttgg ccaaattagc atcagactat
    22561 caaaaaccta gaggaatcac ggtagttgcc cctcaagacg ctgtgccatt tttaaaagct
    22621 ttgccaattg aaaaatttca tggggtaggg aagaaaactg tacctagaat gcatgagcta
    22681 gggatttata caggaaaaga cctatatgaa tgcacagaaa tgatgttgat tcgaaatttt
    22741 ggaaaaatgg gctattctct ttatcgtaaa gtcagaggaa tccatgattc tccagtaaac
    22801 gttacaagag agcgaaaatc tgtgggaaaa gagcatactt atgggcagcc gttgcaaacg
    22861 gaagaagcag tgctgactca gttgcgccaa ctagctgaaa aagtagaaga agcactgaga
    22921 agagtccaaa aacatgggaa aacagtagtt ttgaaggtca ggtacacgga ctattctact
    22981 gtgaccaaaa gagtgacatt gccagaatat atctataaaa aagaagcact cttttatcaa
    23041 gcaagcctta tatgggagga aatcttggga gtagaaaaag gaatccgatt gttagggatt
    23101 actttgacga atctggaccc gatgacttat gaaaacatcg tccttccatt atgggaaaat
    23161 caagaaatat aaaaacagct ttgagagttg aaaccggaaa tccgttttct ctcccaaagc
    23221 tgtttttata tcaagtcttg ctttttgatt tgttcatagg tttctgttgc atccgtacat
    23281 ttttcccaaa tcacaacttg attggattct aaagattttt ggacgtcaat gatccgctgg
    23341 ttgctacttc cgcgaaattg tagttttagg tcacgtttgc ttaattcaaa gcgtccgtca
    23401 acaaggatat cgatttgcga aaggagttct aatttatctt ctgaatcttg tagcaattca
    23461 tcaaaggtat agcctgtcca tgaccagata tcttttgtcg taccgaactc ttttcggata
    23521 cgtcgtacga cttgcagaca aacatctgta ttcaaaaaag gttcgccacc aagtaaagtt
    23581 aatccttgca cgtaatcgtt gcgcaagtca gcgatgattt gttcttctaa ttcaggtgtg
    23641 aatggtttgc catatcggaa attttgtgca gctttgttga agcagccttc gcaggcaaat
    23701 aagcagccac taacgtaaag actgttgcgg acaccttcac catcgacaaa attaaacggt
    23761 ttgtagtcag caatatagtt ttggctgtag tcttttgcta accattcttt tggttttgga
    23821 tttctcataa aaaaacttct ttcatttaag gttcaaagat atagctattg aagataaaat
    23881 ctactcgacc acataaataa gaacaaactg acctaaatcc attgttcaag aaagcttgtt
    23941 cttattttga tagagggcgt tcaaagatac aaagccatcg aaagagggtg gtgaaaaaag
    24001 acttgtcata gcctcttatt ctgaataaac ggtgttcaaa agcttgcatc tgcagtaata
    24061 agcccaacta aacgaaaaat atgaggaact atttttgttt agctattctt attacttgat
    24121 gcaggacagc tttttcacaa cctcatttca tgtgtttcac acgtgaggaa atttctttat
    24181 ggcggccatg taccatcgga cgagcttgcg gatttcctaa atagccacaa gtacgtttga
    24241 ctacatcaca tgtttttgga tcatgatttc cacattctgg acattcgaac cctcgttctg
    24301 tcggtttgaa atcaccttcg aaaccacatt gatagcaatg atcgattggc gtattcgtac
    24361 caagataccc gattttgtca tatgcaaaat cccatacagc ttctaaagct ttcggatttt
    24421 gttggagcat agggtattca cagtagtgga tgaatccacc tgaacaatat tttggataat
    24481 ccttctcaaa ttccaatttt tcaaatggcg ttgggttttt acgcacatca tagtggaaac
    24541 tgtttgtata atattcttta tctgtgatat tttcgatgat gccgaatttt tctgtatcca
    24601 aacggcagaa acggtcagtt agactttcac ttggagttga atagacactg aaatgataac
    24661 catattcatc cccccatgca tctgcattag cttttaattc tttgacgata tcaagtgtga
    24721 aagcttttgc ttctgggttt gtttcccatt cgccaccgaa gaaagctgaa gcaacttcat
    24781 acaatccaat gtagcctaac gatacagtag ctcgacggtt tttgaataat tcattgacag
    24841 aatcggtacg tgaaagacgt ttcccgaaag caccatacat atataaaatt ggcgcatttg
    24901 ctggaatggc ttctttacaa cgttctacac gatagagaag cgcatctttc atgatttcta
    24961 agcggtcaga cagtaactgc cagaatttat tctgatctcc ttttgattcg atcgcgattc
    25021 ttggcaaatt caatgtaaca acacctaaat tcattcgtcc gacatttact tcttgaccat
    25081 tttcgtcttt ccacccttgc aagaatgaac gacagcccat aggtactttg aaacttccag
    25141 ttaattcaac gattttatcg taattcagaa tatcaggata cattcttttc gttgcacatt
    25201 ccaatgctag ttgtttgatg tcgtaattag gatcttcagg gtttaagttc acaccttttt
    25261 tcaaagtgaa gatcagttta gggaagatag ctgtccgctt ttcactccct aatccattga
    25321 ttcgaatctg taaaattgct ttttgaattt ctctttcaaa ccagttttct cctagtccaa
    25381 atcctaatga agtgaaagga gtttgtccat ttgaagtaaa taaggtattg atttcatatt
    25441 ccaaactttg catagcatcg aagatatctt ttttggtttt tgcttttgca tattcttttt
    25501 ggcgttctgg cgaatcgatc cattcttccg cgtcttttag atgtttttta tagttcaatt
    25561 ctgcaaaagg agcaagcaat tcatctgttc ggtcggcaga acagccacca tattgacttg
    25621 acgctacatt ggcaataatc tgtgagattt gtgctgtggc ggtctggata gattttggcg
    25681 attcgacttc ggcattgccg attttgaacc cattgttcag catgcctttg aaatcaatca
    25741 agcagcaatt cgtcatcggt gtataaggat gataatccaa atcatggtaa tggatatcgc
    25801 ctttttgatg cgcatttgct atatgtggtg gcaacatttt cagtccgatt gattttccaa
    25861 cgatcccggc tgtcagatct cgctgtgtat tgaaaacatt actgtcttta tttgcgtttt
    25921 cgttcacgac agtttggtcc ttgttgatta gcttttcaat cgaaaagttg atatctgttg
    25981 ctttactgcg agcaaaatct cgttttgtac gataattgat gtactcttct gctaattcat
    26041 attcgttttt ttctagtaat atatgttcca cgatgttttg aatctcatag attttgacat
    26101 tttcagcaaa ccggcttttg atttcttgga caaccaagtc tgcaatcatt tgaatttttt
    26161 cataagtaag tggtgataat gagccgtgaa ttttcttttc tgctttgatc agtgcttcat
    26221 tgatcttttg ttcatcgaaa tcaacatggc ggccatcgcg tttgatgact ttcatctggg
    26281 atgtctctgg ctgctgagta acgacagttt tttcttcata atttactttc attatcttca
    26341 actcctgcta aattgttctt gtctatcata aaacaaaatc ggggaaggag caactaaata
    26401 tcggctatat atagtattca atgtgctgta taattggatt tctatactat atgtagtgta
    26461 tattttattg tgaaaaaagg tgaaaacttg tagctggtgg atttgttttt tgcatattta
    26521 attttgctga ataaaataat tgaataaaat tttttattca gtccaagtaa aagtgacaaa
    26581 aacataagag caaattattc gtagatatgc tactatgcaa ataaatagaa atgaaaaatg
    26641 aaagaggagg agaagaattg gaaaagatga tcgaagtgaa acatctaagt aaaagctacg
    26701 gcagcaaact ttctcccgtc aaagccttag ataatatctc attcacagta gaagaaggcg
    26761 agtttgttgg gattatgggc ccaagtggtg caggaaagac aacgctgatg aatatcttgt
    26821 ccacgatcaa tttgcctacg atgggcagtg tctcgatcca aggaaaagaa atcacaaaaa
    26881 tgaaaaacag tgagctcagt gactttcgtc gaaaaaaact aggattcatt tttcaagagt
    26941 tcaatttgat cgatacccta aatgcaaaag acaatatcct attgccccta gcagttgaac
    27001 ggatgactaa agaggaaatg gaaaagagag taagacatgt agctcaattg ttgaatatag
    27061 aagaactgct caaacggtat cccgatgaac tttctgtcgg acagagacaa cgaattgcag
    27121 ctgctagagc acttgttgtt cagccacaag tgatttttgc agatgaacca actggctctt
    27181 tggactcgaa gtcggctaca gaattgttga actacttgaa gaccatgaat caaaaagaaa
    27241 aagcaacgat actgttagtc acccatgatc catatacagc aagctactgc gatagaattt
    27301 tatttattaa agatggtgtg attttttctg aagttgtcag aagagggaca agaagagagt
    27361 tctttgagaa agtaatagac atgcaggcga cgattggcgg aggaggaaaa atgaatgctg
    27421 tataaactag ccgttaaaag tttttttcgg cagtcacgag gctatcttgt ttattttttc
    27481 agtttgactt tatccgtaat gatctattat tccttcagcg caatgaccta cgatcaatct
    27541 ttagtcagaa gagcaagtca agatacttcg atagatggtg gattgagttt tggtggattt
    27601 atcattactg ttgttttgct ttttttcgta ttcagtgcca accgattttt cttgaataga
    27661 aggcaaaagg aaataggcat ctatcagctt tttggtatga ataaactgca gatatcaggt
    27721 atatatgtgt tagaaatcat gataattgga ttgttcgcct gtatttctgg gatattactc
    27781 ggtattattt tttccaaact cttttcaatg attttggttc gaatgatgga tatggatctt
    27841 actagtccat tttttatctc cattccatca gtagtagata ctttatttgc ttttttcatc
    27901 atccttttag cagtatctgt atattcaata tggaaaatat ggcgctatcc aatgattcga
    27961 agttttggag aaaaagaaca agcagatagt tcaacaatgc gcttcaggac aaggcaccga
    28021 ttactcgcag tcatcgggtt actactttta acgagcggat attttggagc tagtcatttt
    28081 agggaaatca cttcatggat gatcagttat cattatcttg atacattagt tttctttttt
    28141 ccttttttaa tttttttcgt ttgtgtggtc ggtacttatt tgtttttctg ttatacattt
    28201 cggttatttt tgatcatatt gagtaaatgg aagatttatt actatggtat caattttttg
    28261 ataacgggta acacgcaagt tcatttgctc aaaagttggc gtacaaattc tctgattaca
    28321 gtcattctcg gaatttcttt aatgacaatt ggcggcacgg ctagtatctc tactatttta
    28381 tcctaccagg agaaagtttc ctccccgatg gactatcagt ttgatgttga gacagagaaa
    28441 aaaatcaagc cgattctagc agaggaaggg cagaagataa ctaaagaaat caccttgcat
    28501 tataaagtca ttgggagtat ctatagtcaa aatctcgaag aacctatgtt tcaaactgct
    28561 aatctgatca cggaaacaca atatcaagcc ttcagaaaag ttaatccaga tttgccgaac
    28621 atttctttga aatcacctgg tcatactgtt ttgcttgatc aaatgcaaac tctttttcgg
    28681 aatatttcat tatatggatc tagtattgga ctgccagata atcaagaatt gaagattcaa
    28741 caaatgcttc caagtgtatt aggtgacgaa acaatggtat atctaagtcc tgtactgatt
    28801 gtagatgata ccgtttttga aaaagcaaaa ggtgtggatt atcaagtagt caatgtagat
    28861 gtatcaggtg gaaataatga aaaaatagat gaacgactga gcaatgacct gtcgataaag
    28921 tggctcaatg ctgtttatta ttcttatgat atcgtaaatg gaagtctaca aggagaaatt
    28981 agcacaaaga aacttccgga tgaccaaatg aaggaagcac aatttagcca agaaagctct
    29041 cgtttgaatt tctcttccag ctattcgaaa ttgcgtgcag ttaatcggcg aatcggtatc
    29101 aatatttttg tcacactatt tgtcgggatg atcgtgatta ttgctactgg tagtattttg
    29161 acagtccggc aattagcaga agcagaggaa gaaaaagaaa attatcacct attgactaag
    29221 ctggggattc cacaaagaag gatcagacgg atgatcttcg agcaaaacgc acttaccttc
    29281 tttccaccca tgatattagg gatcactcac gcagtatttg caatcaatgt cttcactcag
    29341 tatataaaaa ctgccgatta ctggttggct tatcttgtga gtggcttatt gatccttatc
    29401 tatcttttgc tttatgtcat tacgagtcag ttatattgtc ggattatcga ggaatgatat
    29461 acggaataag acaagataat aaatagaact aaattttgaa taccatttat ttggaataag
    29521 aggttgtgac a
//
LOCUS       DAZUKD010000025        29144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000025 DAZUKD010000000
VERSION     DAZUKD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.025"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..365)
                     /locus_tag="ACG8N9_000941"
     CDS             complement(45..365)
                     /locus_tag="ACG8N9_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4896559.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            complement(395..712)
                     /locus_tag="ACG8N9_000942"
     CDS             complement(395..712)
                     /locus_tag="ACG8N9_000942"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4896560.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            complement(864..3566)
                     /locus_tag="ACG8N9_000943"
     CDS             complement(864..3566)
                     /locus_tag="ACG8N9_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4896561.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            complement(3629..4906)
                     /locus_tag="ACG8N9_000944"
     CDS             complement(3629..4906)
                     /locus_tag="ACG8N9_000944"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4896562.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            complement(4906..5919)
                     /locus_tag="ACG8N9_000945"
     CDS             complement(4906..5919)
                     /locus_tag="ACG8N9_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4896563.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            complement(5935..6663)
                     /gene="sfsA"
                     /locus_tag="ACG8N9_000946"
     CDS             complement(5935..6663)
                     /gene="sfsA"
                     /locus_tag="ACG8N9_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4896564.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            6840..7169
                     /locus_tag="ACG8N9_000947"
     CDS             6840..7169
                     /locus_tag="ACG8N9_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4896565.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            7353..8000
                     /locus_tag="ACG8N9_000948"
     CDS             7353..8000
                     /locus_tag="ACG8N9_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4896566.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            8015..9541
                     /gene="zwf"
                     /locus_tag="ACG8N9_000949"
     CDS             8015..9541
                     /gene="zwf"
                     /locus_tag="ACG8N9_000949"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4896567.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(9590..12388)
                     /gene="ileS"
                     /locus_tag="ACG8N9_000950"
     CDS             complement(9590..12388)
                     /gene="ileS"
                     /locus_tag="ACG8N9_000950"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4896568.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(12650..13357)
                     /locus_tag="ACG8N9_000951"
     CDS             complement(12650..13357)
                     /locus_tag="ACG8N9_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4896569.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(13378..14160)
                     /locus_tag="ACG8N9_000952"
     CDS             complement(13378..14160)
                     /locus_tag="ACG8N9_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4896570.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(14209..14430)
                     /locus_tag="ACG8N9_000953"
     CDS             complement(14209..14430)
                     /locus_tag="ACG8N9_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4896571.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(14511..15110)
                     /locus_tag="ACG8N9_000954"
     CDS             complement(14511..15110)
                     /locus_tag="ACG8N9_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4896572.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(15124..15801)
                     /locus_tag="ACG8N9_000955"
     CDS             complement(15124..15801)
                     /locus_tag="ACG8N9_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4896573.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(15818..17059)
                     /gene="ftsZ"
                     /locus_tag="ACG8N9_000956"
     CDS             complement(15818..17059)
                     /gene="ftsZ"
                     /locus_tag="ACG8N9_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4896574.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(17082..18407)
                     /gene="ftsA"
                     /locus_tag="ACG8N9_000957"
     CDS             complement(17082..18407)
                     /gene="ftsA"
                     /locus_tag="ACG8N9_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4896575.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(18563..19783)
                     /locus_tag="ACG8N9_000958"
     CDS             complement(18563..19783)
                     /locus_tag="ACG8N9_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4896576.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(19798..20886)
                     /gene="murG"
                     /locus_tag="ACG8N9_000959"
     CDS             complement(19798..20886)
                     /gene="murG"
                     /locus_tag="ACG8N9_000959"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4896577.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(20912..22273)
                     /gene="murD"
                     /locus_tag="ACG8N9_000960"
     CDS             complement(20912..22273)
                     /gene="murD"
                     /locus_tag="ACG8N9_000960"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4896578.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(22287..23249)
                     /gene="mraY"
                     /locus_tag="ACG8N9_000961"
     CDS             complement(22287..23249)
                     /gene="mraY"
                     /locus_tag="ACG8N9_000961"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4896579.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(23275..25467)
                     /locus_tag="ACG8N9_000962"
     CDS             complement(23275..25467)
                     /locus_tag="ACG8N9_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4896580.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(25468..25869)
                     /gene="ftsL"
                     /locus_tag="ACG8N9_000963"
     CDS             complement(25468..25869)
                     /gene="ftsL"
                     /locus_tag="ACG8N9_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4896581.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRRVK"
     gene            complement(25874..26833)
                     /gene="rsmH"
                     /locus_tag="ACG8N9_000964"
     CDS             complement(25874..26833)
                     /gene="rsmH"
                     /locus_tag="ACG8N9_000964"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4896582.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(26854..27285)
                     /gene="mraZ"
                     /locus_tag="ACG8N9_000965"
     CDS             complement(26854..27285)
                     /gene="mraZ"
                     /locus_tag="ACG8N9_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4896583.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(27455..27823)
                     /locus_tag="ACG8N9_000966"
     CDS             complement(27455..27823)
                     /locus_tag="ACG8N9_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4896584.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            27994..28950
                     /locus_tag="ACG8N9_000967"
     CDS             27994..28950
                     /locus_tag="ACG8N9_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4896585.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            29023..29139
                     /locus_tag="ACG8N9_000968"
     CDS             29023..29139
                     /locus_tag="ACG8N9_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4896586.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
BASE COUNT         8174 a         6161 c         4979 g         9830 t
ORIGIN      
        1 ccgttcaaaa atttttcttc ttccgttcaa aaaagaagtt gtatttattc gctgtatacg
       61 cgacgataaa gttcgatgat ttcttttgct aaatcagtaa acgcaataga tgtcattaag
      121 tgatcttgac tgtgaacagt caataatgtc acttgcatat gatttccttg tgcttcttgt
      181 gtcaacatgt ctgtttgtgc atgatgcgct tgtataagtg attcttctgc atcagcgatt
      241 ttcctatccg ctaactcaaa atcaccgatt ttagcagccg caatcgcttc cattgcatcg
      301 ctctttgcat taccgccaaa catgattaac cccatgatgg cttctagatt ttgttgatct
      361 tccacgttgt tataccccca actgcatact tgatttattt cattagattt tctgcttgtg
      421 caagaacttt ttctccgttc atcatgccat agtcttgcat attgatgaca tctaatggaa
      481 tacctttagg cgcaagtttt tgttcaaatt gagctttcat gaaacgtact tgaggaccta
      541 ataataatac atcgacattc ttagattcta aattattatc tgcatctgag gcagagaccg
      601 caaaaatatc ggcttccatc cctttttctt ctgctgcttt ttgcattttt gtgaccaata
      661 aactcgtact catccctgct gaacaaacta acatgattgt tttttttgcc atttttaaca
      721 ctccttttat ttttttacac ttttatataa cgcaagaagc atgccaataa aaaaaagagc
      781 tgaaatagcg ttttcatgct ttttagctct tttttatagt gcgcacacag atgtttagtg
      841 tgtaaacagt atgattagtg tgttcatttt tttattttat tttcaagaaa catttgtgta
      901 agaaatgcga tatcatcttc tggaattttt acctgatatt ttcgttctag tgccatcaag
      961 tttgcccgaa cgatatccat ttccaaacga taaatttttg agaaaataga cagtttctca
     1021 aattgccgat ggctttcttg tttgatcaat ccgtctaaca tgaaggctaa atggataacg
     1081 attccagtat ctactccagg ttccacgata atcgacaaat cggtctggat ctgctgaact
     1141 gtcttttgca atgaacggat cagttcatca atagaaaaga catcgcttaa tgatccagca
     1201 agtgaatcga caattttagc tgtcgggatc tcatcatcag ctatacgctt taaaatattt
     1261 agtttttcat catcaaaaac gtcataggct gaaaaaaatg ggatattttg ataatcaaca
     1321 tcaactgttc ccgcaattgc tttgatctca tattcttcca ataaggcatc gatgtgtttt
     1381 ttgaatgatt cacgttctaa aaactgcatc tggatcagtt ctaccttatt ttgattaatc
     1441 actggcataa tacgctgata taatttggca gctactcctt caccagtaaa gcatgtcacg
     1501 attacggctt ttttacgctg taaagtttca gacttttccg ctttgaactg atcacgtacg
     1561 atcgattcaa aggaaagctg gatattttga taaatatctt ctaagcttct tccaacactt
     1621 gccatccgta tcgcttctat cacaatcatg gtacttgtca tcgtgatagc tttcgtccga
     1681 atgccagtct cttcataaat caaatcactg aaggtgttta atgatcccat atctgttaac
     1741 aagagtaggc cattcgtcaa atgctcttga tagtttttga catattgtaa cacttcttca
     1801 tacattgttt gaacttccat aaccagtggc atattaaaag ctttccctac ttttgttcct
     1861 aatagttctt gtgccatctc caacatgctt gaagcagtct gtctgccgtg catcaatatg
     1921 agaacatcta ctttgttttc tttaacaggc tcactttctc ctagattgat agaaagaaac
     1981 atactaataa agccgatttc atcaaaagga atctcaactt gttcttcttc ttcaatgatc
     2041 gttgaaagat cgattgctac ctggaattcc ctcttaaaat ttttccgtac gttattcaaa
     2101 tctgggtgga cgatcatatg accttctctc acacgatcca atgtactttg cagatgcaaa
     2161 gcaaaagcaa aacgagcttt ttcattataa atacggtcta accgtttttc agccacatca
     2221 tacaattgat tggttaaccg ccagatttca tctggcaaaa gatctttggg aatcgaactt
     2281 ttagtcaatt ctttgacata tgtctggaaa taagcatcca catcttttga aatgagggtc
     2341 tctagatcaa tgttctccac accggtttcg gaaagagaag tgactttttc ctcaatatcg
     2401 ttatagacct gcatgtttcg ttctggatct tgtgaccaga caacatcagc aaatcctggt
     2461 tcaaatgtca aatactgact tttgctgtcc atgaatcggt cgagacgttc cggtatttcc
     2521 ttgattttca gtaatccttt ttgtacttga agggaaagtt cttcttttcg aatgattagc
     2581 tttttctcat gatgagtacg gtaatgtaaa aaagattttg cacagactag tttcaagtct
     2641 cgtttcactt gaccgatatt tccttccgca tcataaagca tgaaggcaag aatcgcttct
     2701 ttttcgatat cgatacgttg attcaaacga ttcgcttctt gtttgataaa aagagagatg
     2761 atttcataac gctcatccag tgaacgtgaa gcaagattcg gcaaggtgat tgccatagga
     2821 atccgtcgat tgaacgtaga taagaaactt tctgaagatt cagtcgtcgc cccaatgatc
     2881 tgaacagatg cttcatgtac ttgtgcactt tctccaagcg gacggtaaat acctttatca
     2941 atgaaagtaa acagcatttc ctgcccttcc ggcggtaaac ggtggatctc atctaaaaac
     3001 aatataccgc cgtctgcttt ggctattaga ccgggactat cttgagctgc tcctgtgtaa
     3061 gccccttttt tgatcccaaa gatgtgtcca aagagcagtt gcggattttg agcataatcc
     3121 gcacaattga aagaaacaaa tggcgcatct gcagaaagca tctctgaatc gattgcaaaa
     3181 tgatacatac attcagcaaa catcgacttc cctgtaccag tttctccaaa aataatcgta
     3241 tgcaaccccc gcggtggata aagaatcgca gcttttgcct gctgtatgct gacttttaaa
     3301 gaatcatttt ctccaacgag tgtttcaaaa gtcacttgcg tagaagagtc gacatgcact
     3361 tcatcagatt ttgacttttc cgttggcaaa gagtaaacga ctggtcggcc agcggttttt
     3421 acaatatgat gggccttgta tagttcactt aaatatcggc ttgcattgct tcgatcgata
     3481 gttaagcgct cagctacatc acttgctgtc aatccttgtt ggttttctgt caatagctgc
     3541 aagatctctt cttgacgttc tttcacataa cccctcctct ggcaaccaat tttgttcccg
     3601 ttttcataat catagcataa aaaggcgctt atttgcgcct tgctccttta tttctctctt
     3661 tttttgattt atttttaact ttttttctta ttttttcttt gttatattct gggttctgct
     3721 cctttccaga aaaagttttc gcatactttt tatttactgc aggagctgca tcttctttgg
     3781 ttggctgctc agtttgcagc ttacctccgt ataaaaaaat ctcttcaagt gttatatttg
     3841 atggacgtaa aagccgtttt aaatctctta gattattatc ttgtacgagt gtaattacta
     3901 ttccttcatt acccattcga ccagttcttc ccgaacgatg caaataagct tcttcactta
     3961 acggaacttc tgcattcact acataaggaa gacctgtaat atctaaacct ctagccgcca
     4021 catctgtggt caaaagtgct ctgaccattt tctttttgaa ttgttctagc gctgcttttc
     4081 ggagtagctt gttctgatca gaggccagac ttcctaccgg tacacctcta tacagcaatt
     4141 tttcttccat tacgcctaat tcagtcagtt gattaaagaa aatcagacca tgaaaatctt
     4201 ctatatgcgt gagacgtctc aagtagtcat ctttctttcg tatcggtaca cgtaaaaagt
     4261 aatgtctcag tccttttcga gaatcgtcct ctgctgtcac gtcaattgtc tcaaacgttt
     4321 tcccagtgat tttttcaatg ctttcaaggg aacgatctgc tgtcgctgaa aaaaaagcta
     4381 gctgatactc tgttggagct tgatgaagaa tttgatcaat gatttggctg ttcccagcgt
     4441 caaatagctg gtctgcttca tcaaatacca ttgttttcac ttgatgtgct tttatttttt
     4501 ttgctttcat caattccaat attcttcctg gtgtaccaat caagatttct ggtttttcct
     4561 tcaaaccctc aatctgacgt ttgacgttgg ctccgcccac caaagattgg actttcaact
     4621 ctttgtcttt tccccattca cgtgcaacat ctgctacttg aatagctagt tcttgagaag
     4681 aagcaaaaat gaccaacgca cttgcttcac ctggttccac gtttagtagt agaggccaaa
     4741 gataagccag tgttttacct gaacctgtag gagaaaccgc tactatactt tgtttattac
     4801 ttagtggctg aaaaactgct cgttgaataa gagaaggtga gataaaccct ttatcctcat
     4861 attttttctg ccaattttcg ggaaatactt gtacaaattc gttcattaaa aatcttcctc
     4921 attatctgca tcgaatttga tgccgccatt ttttcttaaa tcataaatca cttggttcac
     4981 atttctagct agctcgatcc actcttcata caacaatccc caagctggat ctttcggatg
     5041 agccatgaca ttggcaaaat cctctgcttc atctgccatt ggattttctg cagctgttac
     5101 cgccaaatca atgatttctt tatgcgcgcg atcatgaaat tgtgcttgtt caattgcatt
     5161 gactccattc atcactagcg ttcctgagtc aaaataaatc tctgacggta aataagaatc
     5221 accgatttta cctggctgga tcgatacatc aaaattctca taacgtaaga tcccccatcc
     5281 aagaccatct acaccagtag aaatctttct tgggaaataa tggacttctt ttggcattcc
     5341 aaaccaagca acagctgcat atacaaggta aacacctaaa tccatcaatg caccacctga
     5401 gaaatgagtc gagaagatat tcggttcttc tccatctaat acttggtcat agcgcgaaga
     5461 atatttcata tagctgaaat ttgcaccgat tatctcgtct tttaatggta agtaatcggc
     5521 aatcttttta aaacctgatt catggatatt tcttgctgct tcaaaaaaat acacattgtg
     5581 ttcatttgcc aaatggatga tttcatccat ttcttttggc gtcgaaaaag ctggcttttc
     5641 aactatcaca ttttttcctg ccaaaattgc ttgcttagct tgactaaagt gaagactatt
     5701 cggagaagca atataaatgg tgtccatatg agtaatattg aaaaaagtct tcaaatctgt
     5761 tgcgaattcg atatcccctt cataatgaga accaaattcc tgtgcttttt ccaaatgtct
     5821 agaatatacc gctgtcaatt catatttttt tgtgcttaaa gctgcttgga caaattgatg
     5881 agtaatccaa tttgttccga taatacctaa gttgatcatt attttacctt ctttctattg
     5941 tataggatct tcaaaaggga tatctacatc aaatggaatg gcctgcttga cagttacctc
     6001 atctgccgat acttgacaat tataggcgag gatttgaact ccgctttgct gcgcatgtcg
     6061 aaccatatca gctagtgctg gttgcatttc ttcatggatc gttgccagat gaagatgttc
     6121 gaactgagca ataaacaaga cataagcggc ataaccatct tgtgcggcat acgttaattc
     6181 tcctatatgt ttcaatcctc tgagtgtggg cgcatcagga aaagcaccaa tcgctttatt
     6241 ttcaagtgtc atacctttga cttcaacaaa tgctttttgt ccgaccaccg tctctaaata
     6301 aatatcaaat ttggagtgaa gataagttac ttcacgcttg tataccgtaa atggaccagt
     6361 taatcctggt aaaaggatcg tgccgtctaa aataccatca attgccagcc gattaggcaa
     6421 ttgactatcg atattaaacc agtcaccctt cttttttaca gcaatcagat cataggcggt
     6481 tttccgttta ggtccaggag caaatacaat agccacttca gcgcctggta tcaggacctc
     6541 ttttcctcta cctgtatttt tgacatgcgc aataattatt tctccgtctt ttttcataca
     6601 atgtgctata aaccgattcg ggcggtcaat aaaaaaagct atttccactt gctgatactt
     6661 caaattatgt cacctttctt cctactatgt atcctatcat aatttgtaga aaaacagtaa
     6721 aaaaaccagt cgaaagtctt agagaaattt tagcttttat tcggtacaat gaaataaaga
     6781 agaaagaata tgtctatgcg attattttct cataaaggag tgatttggtg ataaaaaaaa
     6841 tgatctattt tcttggttca ctcattaccg ttctctcaag ttttatttac ttgaaaaaga
     6901 aaaaacaagc ggaaaagtca gaaggactct caaagtatca aggtacatgg ttctttagtg
     6961 atagccagca aaaacaacaa ttacagatta catctgatta cacgtttttg atcaacggtc
     7021 agtctcttgg cacatctgtg gtagaaatag atgaatatcg tttagtcgta cgtgatcagt
     7081 atggttttta tcttatcttt tcttatcccg aacaaactct ttatgacgaa tctgcagaca
     7141 cttcatataa gttgttatct gaaaaataga tccctcccaa tgtatatata tatcctttat
     7201 aaacgaaaga atgagtcttc tctatcatct ttcgtttttt gttgtaaaaa cgtctaaaaa
     7261 tcctttgttt tttatttaga aaaatagtca attaacgttt tttttgttac acttactaat
     7321 gatggttttt taccatgaaa ggatgatcga atatgacacc aaatcgagaa gattatttga
     7381 aaatcatttt agaactaggt ggagatacaa cgaaagtcaa caataaacaa atcgtatcta
     7441 gtctagatgt ctcagctgca tctgtcagtg aaatgatttc taaacttgtg aaagaagagc
     7501 ttgtagaaca ttccccttat caaggtgtac aattgactgc aagcggactg caaaaagcta
     7561 gcagtctgat tcgcaaacat cgcttatggg aagttttttt agttgaacat ttagactata
     7621 gctggaatga agtccatgat gatgctgaag tattggaaca tgtgacttcg gaacatcttg
     7681 cagatcattt agaaaactat ttgaatcatc ctgaacattg tcctcatgga ggatcgattc
     7741 ctaaaaaaga ggaagttgtc catgaagaac gccgacaaac attggagtcc tatcctgtcg
     7801 gtacaaaagt gcgtatcgca cgagtgcttg atgaaaaaga attgttggat tacttagtca
     7861 ctattgacct gaatatcaac gaagaatatg agatcgttga catcgcagct tatgaaggac
     7921 cgatcactat ccaaaatgat agaaagaaaa tccccgtcag ctttaaggcg gccagcacga
     7981 tttttgtcga taaaatataa aggagtgtat ccacatgtct aatgaaaaaa atgtattgtt
     8041 tacgatattc ggcggtacag gtgacctagc ccagcgtaaa ttgtacccgt ctttgttccg
     8101 cctttacaga aaaggaaatt taggcgagca ttttgctgta atcggaaccg ctcgccgtcc
     8161 ctggagcgat gagcactacc gtgaagttgt caaagaaaca atccgtgcat tgaatccttc
     8221 agatgaagaa gcaactacct ttgcaagcca tttttactat caatcccatg atgtaaatga
     8281 ttcaacgcat tatcagacat taaaagaatt gtctgaccga ttagatggac actatcaatt
     8341 aaacggtaac cgtgtctatt atttggctat ggcacctcaa ttttttggaa cgatcgtttc
     8401 tcatttgaaa tcacaaaata ttatgacaaa aaatggcttc aatcgtttga tcatagaaaa
     8461 accgttcggt tctgattacc agtctgcttt tgaattgaac cagcaaatca gagaagtatt
     8521 cccagaacaa gatattttca ggattgatca ttatttagga aaagaaatga tccaaaacat
     8581 ctcagctatc cgttttgcca ataatatttt tgaggctcaa tggaacaatc gctatatcga
     8641 caatatccaa attacttttg gcgaaaacct cggtgtagaa gatcgcgggg gctactacga
     8701 tcatagtggc gcacttaaag acatggtgca aaatcatatt ttacaggttg tagcattact
     8761 tgcaatggaa ccgccagtag cattttccga aaaagaaatc cgtacagaaa agatcaaagc
     8821 attgaaagct atccgcattt ataacgaaga agaagtgtta gaaaacttcg ttcgcgggca
     8881 atatgctcaa ggtgaattgg atggtgtaca attcaagggc tatcgcgaag aagacaaagt
     8941 ggatgctcaa tcagagactg aaacattcgt cgcaggcaaa ttcactatcg ataacttccg
     9001 ttggtcaggt gttcctttct atgtacgtac aggcaaacgt ttgactgaaa aagggactcg
     9061 gatcaacatc gtattcaaac aagttcctgt taatgtattc aaaacatctg tcgatgaacc
     9121 atgtgacgat acgacacttc cacctaatgt tctaacgatc tatattcagc caactgaagg
     9181 attttcttta tccttgaatg ggaaagaagt agggcaagga tttgaaacgg aaccaatcaa
     9241 attggaattt agaaatagtg cagaaatggt tgaaaatagc ccagaagctt acgaaaaatt
     9301 actattagat gcgttgaatg gtgatggaac gaacttctct cattgggaag aagtcgctca
     9361 atcttggcat atcgtggatg tcattcgaaa agcttgggac caaacacaac cggattttcc
     9421 caactacaaa gctggatcaa tgggacctaa agcagctttc gaactacttg aaaaagacgg
     9481 attcgaatgg atctggcaac cagataactg gtatcgtgaa cgtggaaaat taaacgattg
     9541 ataacaaaag ggggtgtgac aaagaacatg tcacaccccc ttttcccttt tacttttctt
     9601 cgaatccttc agctacagct tcaggataga agtcttcaac gatttttgca caacgaccgc
     9661 atagatgagg aagtttttca tctactccta catctttacg aacttggcga cagcgatcgc
     9721 aagtttcccc gtctgctttt tccactgtaa tagcaacatc atcaaatacc atagcgttat
     9781 ctggcacttg ttcatctgct gccataactt caaagtagtc tggtgaaacg atcaataatt
     9841 gtgcaatatc tgcatccaaa gcttttaaca tttcacgaac ttgttgtttt ggataaacag
     9901 ttagttttgc ttccaaagat ttaccaatca atttgctgtt tcttgcttct tccaatgctt
     9961 ttaagacatt atcacggaag tccatgaaag cagtccaggt atccatcaat tcttcttggt
    10021 ttgcaaactc ttcataacca ggaaactcgc ttaactgaac gtactcctct tcttctttca
    10081 ggaatgacca gatttcttct gctgtatgcg gaatgatagg agtcaataat tttgtcaaag
    10141 ctacagctgt ttgatagaaa acagtttgca tgcaacgacg ttgataatca tcttctgctt
    10201 caatataaac tacatcttta gcaaaatcca ggtagaatga cgatagatca accgtcaaga
    10261 agttcataac tgtacggtaa atatgcatga agttatattt gtcgtaccct tcttctcgga
    10321 tttctttgat tacttggttc aatcgaacgg tcatatattt gtctacagaa cgtaaatcat
    10381 tgtacgaaac agtatctttt ttaggatcga aatcttctgt attcgctaat aagaaacgca
    10441 tcgtatttct gattttacga tatacttctg ccacttgact taagatatcc atagaaacac
    10501 gaacatctgc ttcataatct acactagata cccataagcg aaggatatct gcaccaaatt
    10561 gtttgatcac tttttctggt gcaatgatgt ttcctaaaga tttactcatc ttacggcctt
    10621 caccatctaa agcaaatcct tgagaaataa ctgatttgta tggtgctaca ccattgatag
    10681 ccacactagt cgtgatactt gagttgaacc agccacgata ttggtctgat ccttctaaat
    10741 acatatctgc tgggaatgat aattctggtc gctgacgtaa aacagcttca tgagaagaac
    10801 cagaatcaaa ccagacatcc atgatatctt tttcttttgt aaattcgcca ttaggagaac
    10861 ctgggtgggt aaatccttca ggaagaagtt ctttcgcttc tttttcaaac cagataattg
    10921 atccatgttc agcaaataat tttgccacat gttcgattgt ttctggtgtg atgattgctt
    10981 ctccattttc agcatagaaa atcggaagtg gtactcccca tgcacgctga cgggaaatta
    11041 cccaatcgcc gcggtcacgg atcatattat acaaacgtgt tttaccccat gggataaccc
    11101 actctacttt ttctacttca tccaaaatat tctgacggaa tttatcgata gatgcaaacc
    11161 attgtggagt tgcccgatag atcacaggct ttttcgtccg ccaatcgtga ggataactat
    11221 gagtaaagaa atctagcttc agcaaagctc ctttttcttt taatagatca gtgatcatag
    11281 gatttgcttt gtcataaaag ataccttcaa atccaggtgc ttcttctgta aacaatcctt
    11341 ttgagtctac aggtgacact acagatagtt tatatttgct accagcgata tagtcatctt
    11401 ccccatgtcc tggagcagta tggactaatc cagtaccagc gtctaaagta acatgatcgc
    11461 ctaaaatcac aagtgattca cgttcataga atggatgact tgcagtcata tattccattt
    11521 cttgtccagc aaattctttt agaactgtta cattttccca accgatttct gattggactg
    11581 tttctagcaa atctttcgct acgacaaact tacggccatc ggcttccacc aatacatacg
    11641 taaattttgg atttacagca ataccttggt ttgctggcaa tgtccatggt gttgtcgtcc
    11701 aaatgacaaa agctgtatct tcatcaagaa tatctttgcc gtctttgaca ttgaaagcta
    11761 caaaaataga tggagatttt acatctttat attcaatttc agcttccgct aaagaagact
    11821 cacttgacgg agaccagtaa attggtttca atcctttgta aatatagctt ttttcagcca
    11881 ttttaccaaa tacgcgtact tgtgcagctt cataagaagg atctaatgta acataaggat
    11941 gttcccaatc tcctgcaact cctaggcgtt tgaaatctgc tcgttgctta tctacttggg
    12001 aaagtgcata ttcacgacat ttttccaagt attctgccat ggacatttct ttacgtttca
    12061 cgcctttatt agccaatact tgttcaatcg gcaatccatg tgtatcccat cctggaacat
    12121 atggtgcacg atagccagac attgattttg aacgaatgat aatgtcttta ctgattttat
    12181 tcaatgaatg tcctaaatgg atattaccat tggcatatgg aggtccatca tgaagaacaa
    12241 agctaggttt tccttcgttc aactcttgtc ttttttggta gattcgattt tcttcccatt
    12301 ctttttgcca ctcgccttct cggtttggca aattaccacg catagggaat tttgtttttc
    12361 ctaattgcaa ggtttctttc attttcattc aaccatacac tcctttattt gttttccgga
    12421 cacaaaaaag catgttcagc cgcaagggac gaacatgccg tggtaccacc caaatttagt
    12481 tgcatctatc tagtgcaaac tctctgtgac caataacgag gtcagccgct gcttcttact
    12541 ctaatttcgg aaaagcaggt ctcaagtaga ggatctttca ccatgcagtg tcttaccgaa
    12601 cttccactct catcggctcg ctttaagaat aacaaagtgc ttttctgttc tactcgtttt
    12661 cattttcacc aggcacgatc gagatatcaa tagcctgtgt atcaaatgcc gtcataggtg
    12721 cttctatgac tcctgtgtct gagtttacat cagtatcatt ttcattgtca agctgttttg
    12781 ctaaaacttc tttgatcact tcatggctgt cagtcacgta gctggagaat ggttgtaaaa
    12841 tttcatccca ttccggactt ttcacttgtt ctaattgaga ctcaagcatc aagctgatac
    12901 gttgatggaa tactcgtgtt tttttcttaa gatcttcagt ttctgtcgca agctgtcttg
    12961 ctttttctgt tgcatctgtc aagatttttc ttgctttttc ttctgcatca gttgttaatt
    13021 gatgtgcacg tttttcagcg ttcgcaacta actcatctgc tttgttttgt gcagaagtaa
    13081 cgatcacttc ggattccttg cttgcacttg tttttacttt atcagccgta tcttgagcta
    13141 cgatgattga ttggttcaaa gcatctttta attcattgaa gtactcaagt ttttcttcag
    13201 aatgtttgac tgctttttct aattctcgat ttctttgagt caattcctca tagtcacgaa
    13261 caactaaatc taagaaatca tctacttcgt cttgattata tccccgcatt tttgtcggga
    13321 atgtcttatt ttggatatct aatggagtta aagccatgag taaaccacct ttttatttta
    13381 tttacgtaat acaccgaaca gtaccctgta cttttctttt ttcgtcttcc cctcaagttc
    13441 ttggatctgg actctgccat aacctctgac tgaaataata tctaatagtt caagtacaaa
    13501 atcaggtctt gtattttctg tccagttcac ttttacttta ccagcttcga tcagctgctt
    13561 ggaccgctgt ctggaaatat tataaatctc agagatcaca ctgtctagtc gcatggaact
    13621 aactgttcct ctttcttgtg tccagctatc tttcggcata atcatttctg tgtatttgat
    13681 tggttccaag cgtacagcaa cctttccaat ctttgttact tgtgcagcaa caaaatgatc
    13741 cacctcattt gcaataaata cttgccagcg atcaccgtcc gagatgatat cgccaaagta
    13801 agagcgtttg atccccgttc ccactaaagt tccaaggatt ttcccatgac ttaggctggc
    13861 aaatttgcta ggataatgga tttcatacaa tgctgtttca aaatcttcct ctacaggttc
    13921 gtaataatcg gggaaaatca aacatctttt tctttctgcc tgttcatatc cgccataaaa
    13981 catgaaacgc aaatcactgc tttgtcgaat cagtgtttct aaaatatagg attgtcttgg
    14041 gtcaagaaat tcggtcagat agggtgcata ttgtccttga acctgttcaa tccaatcgcc
    14101 taccgaatcg ataaatgtac gttcatctgc ccgaaagtgc tggtatacat ttgcatccaa
    14161 aattttcccc tcctatctat tctgagaata aagattttgt tgcttcaatc aataaactaa
    14221 atggacagtt atccatataa cgatctgtgc taaaccttgt acggctaagt tcaagatcag
    14281 gattgcccct aaaattgtga aatccaaggg tccaatcctc aaaggcagac gatcgaacaa
    14341 actgatatac ggttcgcaga tcttagctaa taatcgtcct agccctgatt gatacccgcc
    14401 tggaaaccaa gagagcaatg catataccat caagataatg ctatagaaat aggctgcccg
    14461 ttgaagtaaa tgaagtaata gtgcaatagt ataaatcgta gttcctcctc ttacagatca
    14521 aacaaattcg catctgcaaa agattgagcg gttgaactgt caatctccat cccagtcggt
    14581 gtacaaagaa aaatctcatc tccaacacgt ttgatatctc catctagtgc ataaactgtt
    14641 cctgttagaa aatcaacgat tcttctcgct tgtttttctt ctactaaatg aaaattcact
    14701 aaggttgctt ctccagcaat gattcgttta gcaatcgcca tagcttcaga ataagctctt
    14761 ggttccatta cagtgatttt tccagcaaga tttgctttag gttgtgtaga ggttcttgaa
    14821 gctgttcttt ctttcgctac agtttttgta ggtgattgat gtagttcaat tacttttttc
    14881 ttttcagccg gctgaatact ttcatgcgtt acaggacgtg aagtcgccgt tttttgtttt
    14941 tcatgagtat tcgcagcaga tacagaatga gttgattgat tctgtgttgt atttttttta
    15001 gttggctgta cagccttttt tgttgtttca ggataatctt ggtattcgta ctcttcctca
    15061 tcagctaatc cgaaaaaatt agcaattgct cctctgttta aaagcggcat ttttgttcct
    15121 cctttattcg tctttgaata gcgctgttcc tacacggata aatgttgcac cttcttcaat
    15181 cgcaatttga aagtcttgac tcatccccat acttaattca ctacatggtg catgggcata
    15241 accttttgct tgaacaagat cacgcaattc tttcagcgta ccaaacactt tatggatttg
    15301 ctcatcagaa gcttctttag gcgccatagt catcaaccca acaaccttga tttttgtgca
    15361 ggcagctaaa gattccacaa acggtaaaag ctcatctggt gaaattccat gttttgattc
    15421 ctcaccagaa acatttactt caataaaaca ggataattct ttctctaacc gcttctggat
    15481 ttcttctgct aatcttaaac tgtccaacgc atgaaagtaa tcaatttcat ttacaatcaa
    15541 ttttaccttt ctgcgctgta aattaccaat taaatgccac tttatttcag gaaaatcaaa
    15601 taaatcttgt ttcttttcta ataatttatc tactcggttt tctgcacaat tcataacgcc
    15661 agctctaacc aattcttcag cttgagcgga ttcaacagat tttgttacag ctactaatgt
    15721 aacttctttc ggatcacgat gaacaagagc acacgaatcc tgaatttctt gcttgatttt
    15781 ctgcaagttg tcagcaatca tgtgctcttc ctctccttta ttttttacga cggaaaaacg
    15841 gtggcgtatt taattcatca tcgccatgag attttgtttc ttcgcggttg aatgtatcaa
    15901 attctttttt ttcgatattg tcaaaattgg aatcatcaac tctaggacga acattttgtt
    15961 cacgtctgat atcccaatcg ccaaaactgt tttcttcttc tgaagaagtt ggtttagctt
    16021 gatccatatc taatacagtc ttttgagcag gtgattgcat ttgtgcttgt ctagctggtc
    16081 ggctagactt gcgttccttt tttgattcat cgatacctgt tgcgatgacc gtcacacgga
    16141 tttcatcgcc catttcttca ttgatagatg tacctagaat gatatttaca tcgccagttg
    16201 ctgcatttgc tacgatatct gaagcatctt gtgcttcgaa caatgtcata tccaaaccgc
    16261 ctgtgatgtt aagaagtact tgttcagctc catcaataga tgtttcaagc aatggagagg
    16321 agattgcttt tttcgttgct tctactacgc gatcttctcc actagccaca ccgatcccca
    16381 tcaatgctgt accttggttt tccatcactg ttttcacgtc agcaaagtca aggttaacgt
    16441 aacctggcgc tgtgatcaaa tcagagatac cttgtacacc ttgtcgtaaa acattatctg
    16501 cttcacggaa agcttcaagc atcggtgtct ttttatctac aacttctaat aaacggttat
    16561 ttgagataat taataatgta tccacatttt ctttcaaacg agcgatacct tctgcagcaa
    16621 aacggccacg tttcggtcct tcaaaagtga atggacgtgt cacgacacca acagtcaatg
    16681 cgcccaattc tttcgcgata cctgcaacga ttggtgcagc acctgtaccg gttcctccac
    16741 ccattcctgc agtgatgaaa atcatatctg caccgtctaa agcttcgcgt aaggattgtt
    16801 cactttcttc tgccgctttt tgtccaactt ctggctgaga accagcacct aaaccacgag
    16861 tatatttggg gcctaattgg ataactgttt cagcttttga attttttaat gcttgcacgt
    16921 ctgtgttggc tgtaatgaat tcaacacctt taacgttttc ttcaatcatt cggtttacag
    16981 catttccacc gccaccaccg actccgatga ctttgattac tgcgccgtca ttgatattat
    17041 tatcaattga gaattccata tcttattcct cccaggatga attaatcaaa aatatttgta
    17101 aagaaaccct tgattttatt ttttacattt tctccggact cttttggttc cggctcatcg
    17161 tagattgttt cttcaggtgc ttcatagctc tcatatgtgt ttgttgtttc ttgttcaact
    17221 accatttgac gagctgctga ggtttcaccc gtaacagcaa tttttgccaa ttgatacact
    17281 tcactcaagt ttgctgaata atccacgata cttattacat ttgtaaatac cgggtttctc
    17341 agccccatgt gatttggaac atacaatttt acatttacgc cgaagatttc ttgtgccaag
    17401 tccacaacac caggaagact agcagctccg cctgtcaata cgacgccacc tggcaactct
    17461 aatgcttcga tttggtccaa tgcatctttt gctttgttga aaatctgttc cattcgagct
    17521 gaaataattt ctgacaagta gcgttcatcg actttcactg gttctgactg accaatcaca
    17581 tctactggaa attcttcatc tggtgatgtc cgttctgggt atgcatcccc gtagtttatt
    17641 ttcaacgctt ctgcattgtt aaatgacgta tttaatacaa tagagatatc tttagtgaca
    17701 aattcgccac cttcttggtc aagattggta aacttcaatt gcttatcatg cataacagcc
    17761 gttgttgttt ggccgccacc catatcaatg acgatcgtac caaaatcttt ttctccgtca
    17821 gaaagaattg attcagtcag cgctaaagga gtaatcacca tttcgttgac gattaatccg
    17881 gcattttcca cgcatttacg tatgttatgg atgatcgttt taggtcctgt aaacaacaga
    17941 ccgtacattt ctaatcttac gccgatcatt ccacgaggat ctttgattcc ctcgaaccca
    18001 tcgacagtaa aatcttgtgg caatattgac acgatttgac gttcaggagg tattgaacga
    18061 acaagggcag ctgaagccac atttctgaca tcttcatcgg tgatttcctt tgaatctccg
    18121 ttaactgcaa tcattccctg gcaattttct acttcaagca tgtttgctgg caaaccgaca
    18181 cttacgcctt tgatctggat acctgctttt tcttctgctt gtcgtactgc tcgttgtatc
    18241 gcttggaccg ttttatcgat atcaacgata atgcctcggt taatcccttc tgattttgcg
    18301 tttcctactc caataatatt catttggctg tcgatatatt cagccacgac aactttaaca
    18361 gatgtcgttc caatatcaag gcctacatac attcctgttt ttgccatgaa tgggattccc
    18421 tcctacttac tattccatat acaagcgaac aactagcgtc cgttttaaaa tatacttatt
    18481 aatagatata ttcactgctt ccaattctac cataaattac tggtttttag aaagtacatt
    18541 cttacttttt aaagttgttt tatcaatttt catttaaact tgacggagtt gtacttggat
    18601 tatcttctat ttgacttgtt tcacttgaat catttgaatc agatgatccg tttgtgctag
    18661 acgatccagg tgtttgtgct ttttgcgttt ctgcctcttc acttgattcg tttgtttgat
    18721 ccgcattttc ttcactttca gttgtttctt ccgattggcc cgattcttcc gtttcttgag
    18781 tgctaccgta aggatgcgag aagataccaa cttccatgtc gatcacacct ttttcatcca
    18841 tatctttggc tacttgcgaa taatatttca tttggttcgc aagattattg atatttacaa
    18901 taacttgatt gccgtcattc atattcaatt ggatcagatt tttattgctt gctcttggcg
    18961 tgtacttgat ttcagaaatt gctttttgta attctgatga taactgattg tattgctttg
    19021 ttaattcttt gatttttgca gagtctttga actcttccaa gatagggaga ttttttgtag
    19081 gatttgctac tttttcatct aatactgttc cattttcaat cactgggtag tattgattgt
    19141 tttttgcgat caaagcaatc tctttatatt ctgttacatc caaatcgaat tcattcatcc
    19201 ctttgaaatg tatatttgct gtttcgattc ttggctgtgc ttttttaagg cgatcgatat
    19261 aagtagatcg atgaaaatac tgtccccaaa cattttctcc taatgacaat tttgtatcag
    19321 agatgatttc ttggctgttg actgtcttat tgcctgatac tgcaactgct tgtaatttgc
    19381 tataaggaga aacataatag atcaaaaaaa tcagcggtat acccaataca gtgatgatca
    19441 aagctaaccg acgaaatagt actttgtgcc gttgactttt taaattgggc agccggttca
    19501 aaaaagaacc gttatatggt ccaccgtttt tcttttgctt gatctcttca aaaaattcga
    19561 tttttttctc ggactggctt tcttcttgct gttcctcttg aaccgttttt tcttcttcag
    19621 aatctgaaag gggtgtatct aatgttatgt cttctttttt tggctcttgg tttttcatct
    19681 cttgttctgc cagatatttt cggttagcct tttgccaagg agtcaaattg ttttcatcag
    19741 aagacggatg agacaatttt tttggttctt ttttcttact cactaagacc accgccttta
    19801 ttttttgatt atttgtttta ctaagtcata aagacgtttt gaagcatcag ggattccttg
    19861 ttctttagaa gctcggcaca tttgcttttg caattcttca tcacccatga tttcgttgac
    19921 tgtctgactt agtgattcac ctgttagttc attatctgca atcatttttg ctgctcctgc
    19981 gtgaacaaga ctcattgcat ttttagtctg atgatcattc gtaacatatg ggctgggaac
    20041 caacactgcc ggtaacccca gagctgtgaa ttctgcgatc gaagttgcac cagcacgccc
    20101 caccagcaga tcactggaag ccatgacctc tgccatattt ttgatataag gctggacact
    20161 aatattttgt atttcaggat ttattttatt taggatctct tcataatatc tatctcctga
    20221 agcatacaaa acttggtaag aagaaggaat atattcactt aaaaaaactg atactgcttg
    20281 attgatttta agtgctcctt gactaccgcc aaaaatcaag actgttttct tttctggatc
    20341 caaatcaaat gttttcaaaa tatctgattt tttagtatct gctacttctt gtgctcgagg
    20401 attacctaca agaactgctt tgttttcagg aaaaaaggaa gccgcatctt taaatgaaag
    20461 agcgatccga tccacatatc ggcttaaaaa tttattggta acacctggta cactgttttg
    20521 ttcatggata actgttggaa tacccatttt ggctgcagcg tacactaccg ctccggaaac
    20581 atatcctcca gtaccgataa cgatatctgg ttgaaaatca ctgaggattt tttttgcttg
    20641 acggatactc ttaagaaaca atcggaccgt cttgatgtta tgcatggaaa tttttcgttt
    20701 aaatccttga atctccaagg tatggaaagg cattcctgta tcagggacga ttttgttttc
    20761 tagacctcgt tttgctccca catacatgaa ttcagcattc ggttcttgag tctttacata
    20821 attgacaaag gcaagagctg gataaatatg tccccctgtt cctccgcctg taaccaaaat
    20881 tttcattttt tatcgctcgc ttctactttt tctattttaa tcggttcaca gcttccatga
    20941 atctgtttcc tctagtttca aaatttgggt attgatccca gctggcattc gctggtgata
    21001 ataaaactac atcaccatct gtactgtttt ctaatgctaa aggaactgct tgttctacag
    21061 aatcggctgt tactattgtc tcgatgcctg ctttttttcc agcatccatc aacttttctt
    21121 ttgtttggcc aaatacgacc attgctttga ttccttttaa tgaaggaatt aattcatcaa
    21181 atgtatttcc tctgtctaat ccgccagcca ataacacgac cttagacgtt tcaaatccac
    21241 ttagagccat tttagtcgct aaaatattgg ttgcttttga atcattataa aattttctgc
    21301 cttggatttc tcctacatat tgcgtacgat gtggtacgcc atggaaaaaa tgcaacgttt
    21361 ctcgtatagc ttcatttgat ataccataca attttgctac agaaatagca gctagtgcat
    21421 tttctacatt atgcttacct ggtacgccaa gttcagatat ttccattatt ttttcttttt
    21481 tatagtatat tgagtaatcg tcagcataca caccgtcttc caacacttct tttgtcgaga
    21541 aaggcagtac gcgtgctttt gttctttgcg ctaattcttg tagttctgac tgattccagt
    21601 taaggatcag ataatctttt tctgtcatgt ttttttgaag gttccattta gctttgacat
    21661 attcttttct tgtaccatga taatcaatat gtgcttcata gatattggta atcaccgcta
    21721 tatgagggcg gaaatctgtg attcccatga gttggaagct agacaattcc atcacaattt
    21781 tatcttctga ttttgcttct tgcgctacac cacttgctgg atacccaata ttacccgcta
    21841 aacgtgcaat tccttgatct gctcctgcat tcagtagtag tcctgtcatc gttgttgtcg
    21901 ttgtttttcc gttcgttcct gtaatcccaa taattgggca ttccgctact tcgtaagcta
    21961 gctcaacttc cgtaattacc ggaataccca tttcttgggc ttttgctact aaaggatgac
    22021 tgtagggaat ccctggattt ttgaccatca atgaaaattc ttcatccaac aattcgattg
    22081 gatgactacc tgttattact tttatcccta aagaaagcag ctcttgtgct tccggatttt
    22141 cgtcaaaagg ttttccatca ttaacggtga caagtgcgcc caattcatgt aataatttgg
    22201 ccgcacttac tccgcttttt gcgagtccta agacaagcac tttcttattt tcatatgttg
    22261 atattttttt catttcccgt catctcctac caaacgatcc ataatgtgat caacgaagtc
    22321 acaatgctga tcaaccaaaa aactgtatca atcttccact ccgaccatcc gcacatttca
    22381 aagtgatgat gaattggaga cattttaaaa attcgttttc ctgtaagctt aaaggaagta
    22441 acttgaagca tcacacttgc tgtttccata acatagatca aaccaatcaa aagcaatgtc
    22501 cattcttggt ttagcattat agaaattgcc gctaaaagtc cgcccaacgc taatgaaccg
    22561 acatctccca taaaaatctt ggcaggtttt ctgttgtaga cgaagaaacc cagtaaacct
    22621 cctaaaacac ttaagcaaat gattaataca tcatactgat gctgatgcca agcaattaga
    22681 ccgtaagctg caaaagagat acttcctaag ccagcaacta atccatcgat gccatctgtc
    22741 agatttactg catttgaaaa tccaacaagc cagaagagag cgaaaatacc ataaatgatt
    22801 cctaaaggta gatctagtcc gaagaaattc aatacacctg gataaccttc agaacggtaa
    22861 acaagataaa agatgattcc ccctagaatc tgtccaatca atttctgctt cgagttcagt
    22921 cccatgtttc ttttcttgaa tatttttata aaatcatcaa gaaagccgat tactccgtat
    22981 aacgctaata caaaaagcaa gatgaaaaga gtaggagtta gctgtttttg ccaagctcca
    23041 acccaaattc ctgtcaaaat agagccgatc agaaacacta agccgcccat tgtaggagtt
    23101 ccggctttgc tattgtgcca ctttggtccc tcctcacgaa tcgcctgacc ttgctttttc
    23161 atttgaaaat aaccaataaa tagcggcatt gcggcaattg tcattgcaca actgctgaca
    23221 atgggaatca atgcttgtgt ccattccatg ctcttttctc tcctatttct cttcttattc
    23281 ttctaaagta aaactaagtt tgtcttcagt gattttttca tatggagcaa gttcttgttt
    23341 gacacaataa ccatctccat caaaactaac ttcaataccg aggattttcc ccagcttcat
    23401 gatatctgct tttgaccatc ctgttacgtc aggcatcagc ttatcaccac cagtatatag
    23461 gatcagtttt tctccagaga tcaactgatc accgttggca gtagattgtt tttgtacttt
    23521 tttaccatta ccgatcacta caggttgcag tccacttttt tgagcatcag ctgctgcaac
    23581 gtccgcttca agattacgat aatcggctac ggatactttt tctgtttttg tttctgtatc
    23641 tgaatcttct tcagtttctt taaaatccat tgcccgtttc atcaatggat ttgcgatttt
    23701 agccaatgcc attcgatcat atgtcttagg gtgtttcatt gttagataca agacatagtc
    23761 tggatcttca gaaggaacca tctccacaat cgaatacagg taggctgtat ctcctgtttg
    23821 atatccccct gtatcagaag cgatttgggc tgttccggtt tttgccgata cattgtatcc
    23881 aggtacacta tatacaccat aagcactacc gtagttttcg ctttcgacaa catctctcat
    23941 atattcacgg actttttctg ttgtttcttt tgaaaaaggt tgccctagaa cttctgtttg
    24001 agttgttcgc tcttctccag tttggggatc caccactttg ctgatatatc gaggttgaat
    24061 cattgtaccg ttattcgcaa tagaagtgaa agctttcatc atttggaaat tcgtcacacc
    24121 taccgcttgt ccataagcac tcattgctcg gtcgacaata tttaatgtag gtaaagcacc
    24181 gtttacttca tcatctaatc cagaatgagt actttgtcca aatcccagtt tttgtaaata
    24241 gttataccat cgtccgccta atcgctgttc cagtatgacc atcccgacgt tacttgacca
    24301 tgaaagagct tgacgcatgg tcagcacacc tttttcacca aagtcatggt cattgatcgt
    24361 agcatcttct acttggattt ttccagattg gaaggtttca ttctcgttga attctccttc
    24421 ttcgatagca gctgcagtag tgaaaacttt catggttgat cctggttcat aactatcttg
    24481 taccaagaaa ttacgccaga tagcatcttt tccagtcaat ccttccattg tttcaggatt
    24541 gaaagtaggt cgttgtccca ttgccaagat ttctccggtt tttgctttca tcaggactgc
    24601 tgtcaattct tctggctgat attcttcatt tacttgatcc atcagtgttt ctaaataact
    24661 ttgtagtctg ctgtctaaag tcgtgtagat atcttgtccg tcgacagctt tttcttcttc
    24721 tgccactgta cctggcagag gattttgaaa attatctttt tgataaataa tcttaccatc
    24781 tttaccgctc aagatatcat tgtaagcaga ctccaaacct agtttaccaa ctaagccatt
    24841 ctcatctttg tctggaactg cgtatccaat aaaatgagaa gaaaagacgc catttggata
    24901 catccgagcc tgatggtcga caaaataaag accagcgaca ccttcttttt tcatgtccgc
    24961 ttcgatgttt tgcttggttt cttgggagat atttttccca tagctaccaa attcaacttg
    25021 atacagctct ttttttgcgc cgtcctctaa aatattcagt gcatcttttt tgtctataga
    25081 aagattctta tgcaggattt ctgctatttt atcaaaattc ttttcttcaa catacagttt
    25141 tttatcccca gaggtatatg tttttgagag tatcgcttta attgaataag aggaggcatc
    25201 ttctgcgagc gccacaccgt tacgatcata aatcgttcct ctttttgctt ttactacttc
    25261 acttccttgg tacaactgtt ttgttttttc agctaatgag gtaccagcaa catgacctcc
    25321 aataacgata tacgagaaac ggacggcaaa taaaaagaac aatccaatac tcgtagcaaa
    25381 taaaatgata cctacttttt tgcgattgtt catcggattt agatttttct tttccataaa
    25441 ccgccgaatt ttatttttaa gactcattta cttcactctc cttaaattgt cgtcattaat
    25501 agacaatcct ttttcttctg cgatttgttt aatccgttca gtacgagaaa gttcgttttt
    25561 ttcttgttct aaactcgttt tttcttgctt tttatcattg atttctgttt gtattgacgt
    25621 gatttctttt tcagctccgc tgatatttgt gcgcagcata actgttaaaa cacataaagc
    25681 aacaatagca atcgccacgc ttgccacagc aattttttcc aaagcagaaa tctgctttaa
    25741 tcgttttgcc ggtgattgtg gaagaatgat ttcttgattc gaagaagaat caggcatttg
    25801 ttgttccggc tgatccacca attcaggtaa atcatattcg taatcttgca ttttctttaa
    25861 ttctgccatc attctactcc tctttcattc ttattttttc tgcaatacgt aactttgcac
    25921 tgcgtgatcg attgttttct gttagctctt cctctgaagg aaggattggt ttacgagtaa
    25981 tgactttcag ttctggttgg aattcgtcag gtatgacagg aagtcccggt ggtagatctt
    26041 gtaccgtact atattcttta aacatacttt tgacaattct gtcttccaat gaatggaatg
    26101 tgattacgct gattcttcca ttgactttca ataaccggat tgcctgttct aaagaggcct
    26161 cttcggcacc gagttcgtca tttactgcaa tccgcacagc ttgaaaaatt ctttttgcag
    26221 gatgcccgcc ttttcttctt gcgggagctg ggatgacttg tttaatgatt tcgacaagct
    26281 caccagttgt ttggattggt tttgtttttc gtactcgttc gatttcacga gcaatttgtt
    26341 tggaaaattt ctcctcgcca tagcggaaga aaatctttac taattcatgg taggaatatt
    26401 cattaattac gtgataagcg gataaaggag ctgactgatc catgcgcata tccaacggtg
    26461 catcttgatg ataactaaat ccacgttctg cttcatctag ctgaggagag gaaaccccta
    26521 gatcatacaa aatgccgtct accttatcta catgctcact caagcgttct tctaattctc
    26581 tgaagttcgc tttgataaat gtgaccatac cttgttcgac ataagaagct aaacgcagct
    26641 tggcgtgatc gattgctttt tgatcttggt caaacgcata cagatgccct ttagaactta
    26701 gttggcttaa taaatattca ctatgtccgg caccgccgag tgtacagtct acataaacgc
    26761 catccggttt gatgttcaat ccatctaccg tttctttcaa catgactgta tagtgttgaa
    26821 atgtttcagt cattagtcag cctcttccta ttcttatagt ccgaaatcaa tcatactttc
    26881 agcaatttcg tcaaagttct cttctgctgc tgcagaaaaa tcttgccatc gtgtctcatc
    26941 ccaaatttca attcggttag atacaccgat gactacgcaa ccttttgtca aagctgcata
    27001 atttcttaac gtgctgggta tattgattcg tccttgttta tcaagttcgc attcagtggc
    27061 agccgaatag aagaatcgca caaaagtacg tgcgtctttt ttagccaacg gcatttcgtt
    27121 cagtttagct tccagatttt cccattcgct catcggatag ccgaaaagac agccatccaa
    27181 tccgcgtgtc agaacaaact tttcgccaag ttcttcacga agttttgagg gcacgattaa
    27241 acgtcctttt gcatcaatat tatgttggaa ttcacccatc aacattgaaa gccccccatt
    27301 cctccactaa ctaaaattag tctaccacaa tcccccactt tctaccacct tttctgggaa
    27361 atccgggaac aacttttttt attcataaac atttttttaa aaatcaagct gcaaaaagcc
    27421 ttttaaaaga ctttttacag cttttctatt tttttcatag gtaagaaagt atgttcacaa
    27481 tgatcaagac agcgtacaag acgattgtca acagaaaaac caaacgccaa gtcatcttga
    27541 aataacgcgg ataaataatt tcttcataat aatatgcttg gaaaacagca atacaaattc
    27601 ccattagcaa aacagaaata aagaaaaacg gtaagataga aattgtccct gtatttctag
    27661 acaatgcatg cataccgccc cacaaaaaag gaatcgccaa atcaggggct tttattttaa
    27721 aacgacgatt taacgaaaat aattttacaa gaaattggca accaaacaaa acgattaccg
    27781 ggtaaatata ccaaaacaag ataagtggtg tgaatgtgcc catgaacgta aactcctttt
    27841 ttatcgttgc ttcaagtata ctgtatatac tttacacaac tgtttcattt tatttaaatt
    27901 tatgcagtta tattctatat cttacaaaca cattccatgt attccaattc cctatccgtt
    27961 ataataaaag aaacaggcaa caggaggaaa atcatgatca aatattttac attagataat
    28021 gagaaactgc gtaataccgg aaaggaaact gaccaaacga tctggtattg tgtagaacgt
    28081 cctaatcaag aagagattca tcagctgaca catcaatttg aaattccgtc agattacatc
    28141 acttccatat tggacgatgc agaaaattcc cgtacagaag aattcaatca atcaaagttg
    28201 acaaagccag cgttattatt gttacaatat ccttttgcta aaacaagtcc tagcggttat
    28261 tttcaagtgg atacataccc gctctctatt attttgacgc ccaaaaagaa gcttatcaca
    28321 attacgaatc atgaaccact gtttttaaag aaagtcttta gccagacctt tccaaaaagt
    28381 gatatttcgc ctaatctcaa tatcttcttt caattcattt ggcaggtcac actctcttac
    28441 aacagttatt tagctacgct tcaatctcaa tgcgatactt tggaagccaa acttcaagta
    28501 tccactgaaa acaaacaact ttatcaaatc atggatatcc aaaaaagcct cgtttatttc
    28561 agagaagcga cttctgctaa tttagatgca ttgaaagaac tagcgcaaaa taaacttttc
    28621 caagaaaatc atgctttgaa aaatcattta cgtgatgtat tggtggaaac agagcaggca
    28681 atgactactt ctcgcattca attaaaacta gtagaacaaa tgaatcagac tttttcagca
    28741 atcgtatcga ataatctgaa taatattatg aaaattttaa cttctttaac gattatttta
    28801 actataccga caatcgtcgg cagtatctat ggaatgaaca ttaagttacc tattgctgaa
    28861 agggatgatg cctttatatg gctagttcta ttaacattat ttatttgtat cgtaactacc
    28921 tattatttaa aaaaagggaa gtttttataa aaaagccctc ttgtggaaat ggaaaaaaga
    28981 ttgtacactt gaatcaacta attaatgacg aggttgaaaa caatgcgtga aaaaaaacaa
    29041 acaagtactt ttgctaaagt aacaaaattt gtcatttgga caatgctgat tttaacgatt
    29101 ggatctgttg tattgactgc tgtaatgagt gtcatgtaaa aata
//
LOCUS       DAZUKD010000026        28374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000026 DAZUKD010000000
VERSION     DAZUKD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.026"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      142..239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            355..936
                     /locus_tag="ACG8N9_000969"
     CDS             355..936
                     /locus_tag="ACG8N9_000969"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4896587.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            939..2246
                     /locus_tag="ACG8N9_000970"
     CDS             939..2246
                     /locus_tag="ACG8N9_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4896588.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2662..3150
                     /gene="purE"
                     /locus_tag="ACG8N9_000971"
     CDS             2662..3150
                     /gene="purE"
                     /locus_tag="ACG8N9_000971"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4896589.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3143..4267
                     /gene="purK"
                     /locus_tag="ACG8N9_000972"
     CDS             3143..4267
                     /gene="purK"
                     /locus_tag="ACG8N9_000972"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4896590.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4280..5575
                     /gene="purB"
                     /locus_tag="ACG8N9_000973"
     CDS             4280..5575
                     /gene="purB"
                     /locus_tag="ACG8N9_000973"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4896591.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5755..6132)
                     /locus_tag="ACG8N9_000974"
     CDS             complement(5755..6132)
                     /locus_tag="ACG8N9_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896592.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6388..7116
                     /gene="purC"
                     /locus_tag="ACG8N9_000975"
     CDS             6388..7116
                     /gene="purC"
                     /locus_tag="ACG8N9_000975"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4896593.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            7116..7370
                     /gene="purS"
                     /locus_tag="ACG8N9_000976"
     CDS             7116..7370
                     /gene="purS"
                     /locus_tag="ACG8N9_000976"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4896594.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7372..8043
                     /gene="purQ"
                     /locus_tag="ACG8N9_000977"
     CDS             7372..8043
                     /gene="purQ"
                     /locus_tag="ACG8N9_000977"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4896595.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            8044..10266
                     /gene="purL"
                     /locus_tag="ACG8N9_000978"
     CDS             8044..10266
                     /gene="purL"
                     /locus_tag="ACG8N9_000978"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4896596.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10251..11690
                     /gene="purF"
                     /locus_tag="ACG8N9_000979"
     CDS             10251..11690
                     /gene="purF"
                     /locus_tag="ACG8N9_000979"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4896597.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11713..12765
                     /gene="purM"
                     /locus_tag="ACG8N9_000980"
     CDS             11713..12765
                     /gene="purM"
                     /locus_tag="ACG8N9_000980"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4896598.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            12762..13340
                     /gene="purN"
                     /locus_tag="ACG8N9_000981"
     CDS             12762..13340
                     /gene="purN"
                     /locus_tag="ACG8N9_000981"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4896599.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13337..14872
                     /gene="purH"
                     /locus_tag="ACG8N9_000982"
     CDS             13337..14872
                     /gene="purH"
                     /locus_tag="ACG8N9_000982"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4896600.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14888..16162
                     /gene="purD"
                     /locus_tag="ACG8N9_000983"
     CDS             14888..16162
                     /gene="purD"
                     /locus_tag="ACG8N9_000983"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4896601.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLTGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16284..17198
                     /locus_tag="ACG8N9_000984"
     CDS             16284..17198
                     /locus_tag="ACG8N9_000984"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4896602.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17331..18896)
                     /locus_tag="ACG8N9_000985"
     CDS             complement(17331..18896)
                     /locus_tag="ACG8N9_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896603.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18889..20340)
                     /locus_tag="ACG8N9_000986"
     CDS             complement(18889..20340)
                     /locus_tag="ACG8N9_000986"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4896604.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20354..21370)
                     /locus_tag="ACG8N9_000987"
     CDS             complement(20354..21370)
                     /locus_tag="ACG8N9_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4896605.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21590..22678
                     /gene="chvE"
                     /locus_tag="ACG8N9_000988"
     CDS             21590..22678
                     /gene="chvE"
                     /locus_tag="ACG8N9_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4896606.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22732..24258
                     /gene="mmsA"
                     /locus_tag="ACG8N9_000989"
     CDS             22732..24258
                     /gene="mmsA"
                     /locus_tag="ACG8N9_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4896607.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24260..25465
                     /gene="mmsB"
                     /locus_tag="ACG8N9_000990"
     CDS             24260..25465
                     /gene="mmsB"
                     /locus_tag="ACG8N9_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4896608.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25722..26699
                     /gene="guaC"
                     /locus_tag="ACG8N9_000991"
     CDS             25722..26699
                     /gene="guaC"
                     /locus_tag="ACG8N9_000991"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4896609.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26963..>28374
                     /locus_tag="ACG8N9_000992"
     CDS             26963..>28374
                     /locus_tag="ACG8N9_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4896610.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEK"
BASE COUNT         9080 a         4798 c         6457 g         8039 t
ORIGIN      
        1 acaaaaaaat aagtaaacca cgttgtcagt ttcagaaagt agtgttagac tgttttcaat
       61 atttgaaaca acatttttta ttcaaatatg gcaaactgcc ttcacgtgca aaacagttgc
      121 aagaaaatga atttacacta ttattagttt tacataccta cttatatatc gtcataatat
      181 ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga ttggctattt
      241 cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa catagtaggt
      301 ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa taaagtgaaa
      361 gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt attaaaagtc
      421 gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg aaaacggttt
      481 gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc ttctgggatc
      541 gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc tagaaaagca
      601 aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt tacgaaacaa
      661 gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa agtattgatt
      721 atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt atgccagcaa
      781 gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca agatggacgt
      841 cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc ttccttgacg
      901 aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa acacaaaatg
      961 gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga gcagtagcgg
     1021 tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact tatttgattt
     1081 caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt aaccgatttt
     1141 tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct ccactgatct
     1201 tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct tctgggatct
     1261 ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct cctatagtaa
     1321 caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc gagaaaatgg
     1381 gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta cttgcatttg
     1441 tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt cgttcaatcg
     1501 cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga atgattgatc
     1561 tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat tttggtaaac
     1621 caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc agcatggtag
     1681 aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt ggagaagaag
     1741 aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt attttcaata
     1801 cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg ggaatcaaaa
     1861 caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta ttccctaaaa
     1921 tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg ttagtcatgt
     1981 tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc agcaatgata
     2041 aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat gttacgccga
     2101 cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc atcgtaatga
     2161 gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa gatgaagaaa
     2221 tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa tcagcgtttt
     2281 gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc gtttgtttga
     2341 gctaataccg agggtcagct gattgaacac cgttgaagga agaggtcgtg agcctgccgt
     2401 tcatctccga gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg ttgaatattg
     2461 ttccttgtga ccgaggagtt ggcaggagaa caccgtttat ccataaaaag gaatgtgacc
     2521 taacttttgt cacattcctt ttttttatct ttaataaaca cgaacaataa aatgtttttc
     2581 tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta
     2641 gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca
     2701 gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa
     2761 aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct
     2821 cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt
     2881 atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta
     2941 aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact
     3001 acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc
     3061 atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact
     3121 gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat
     3181 cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa
     3241 agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag attggcatat
     3301 tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat
     3361 tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt
     3421 tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt
     3481 tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat
     3541 ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta
     3601 tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct
     3661 tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat
     3721 catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca
     3781 ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga
     3841 ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg
     3901 ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc
     3961 tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat
     4021 tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat
     4081 ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga
     4141 ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat
     4201 cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg
     4261 ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg
     4321 gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg
     4381 ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt
     4441 tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac
     4501 acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag
     4561 tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg
     4621 ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac
     4681 agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact
     4741 tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg
     4801 agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat
     4861 tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatttcta ctcaagttct
     4921 ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga
     4981 acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt
     5041 ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc
     5101 agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa
     5161 tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc
     5221 tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct
     5281 aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag
     5341 ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct
     5401 tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga
     5461 agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta
     5521 tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag
     5581 agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc
     5641 cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat
     5701 tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc
     5761 gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc
     5821 gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt
     5881 gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata
     5941 aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa
     6001 tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt
     6061 atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat
     6121 tgcatcttcc atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta
     6181 atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg
     6241 ataaaacacg aacattaaaa gaatctaatg ccttaattgt acatattttt gttgatttat
     6301 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
     6361 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
     6421 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
     6481 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
     6541 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
     6601 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
     6661 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
     6721 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
     6781 atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg
     6841 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
     6901 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
     6961 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
     7021 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
     7081 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
     7141 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
     7201 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
     7261 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
     7321 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
     7381 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
     7441 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
     7501 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
     7561 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
     7621 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
     7681 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
     7741 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
     7801 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
     7861 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
     7921 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
     7981 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
     8041 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
     8101 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
     8161 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
     8221 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
     8281 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
     8341 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
     8401 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
     8461 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
     8521 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
     8581 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
     8641 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
     8701 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
     8761 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
     8821 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
     8881 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
     8941 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
     9001 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
     9061 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
     9121 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
     9181 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
     9241 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
     9301 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
     9361 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
     9421 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
     9481 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
     9541 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
     9601 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
     9661 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
     9721 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
     9781 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
     9841 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
     9901 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
     9961 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
    10021 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
    10081 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
    10141 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
    10201 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
    10261 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
    10321 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
    10381 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
    10441 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
    10501 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
    10561 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
    10621 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
    10681 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
    10741 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
    10801 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
    10861 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
    10921 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
    10981 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
    11041 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
    11101 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
    11161 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
    11221 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
    11281 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
    11341 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
    11401 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
    11461 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
    11521 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
    11581 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
    11641 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
    11701 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
    11761 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
    11821 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
    11881 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
    11941 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
    12001 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
    12061 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
    12121 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
    12181 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
    12241 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
    12301 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
    12361 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
    12421 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
    12481 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
    12541 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
    12601 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
    12661 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
    12721 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
    12781 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
    12841 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
    12901 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
    12961 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
    13021 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
    13081 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
    13141 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
    13201 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
    13261 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
    13321 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
    13381 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
    13441 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
    13501 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
    13561 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
    13621 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
    13681 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
    13741 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
    13801 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
    13861 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
    13921 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
    13981 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
    14041 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
    14101 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
    14161 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
    14221 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
    14281 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
    14341 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
    14401 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
    14461 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
    14521 tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg tgaaatctaa
    14581 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
    14641 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
    14701 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
    14761 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
    14821 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
    14881 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
    14941 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
    15001 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
    15061 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
    15121 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
    15181 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
    15241 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
    15301 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
    15361 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
    15421 tagtagcaaa aaaatcgtcg tagaagagtt tttgacagga gaagaattct cccttttggc
    15481 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
    15541 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
    15601 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
    15661 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
    15721 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
    15781 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
    15841 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
    15901 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
    15961 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
    16021 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
    16081 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
    16141 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
    16201 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
    16261 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    16321 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    16381 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    16441 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    16501 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    16561 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    16621 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    16681 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    16741 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    16801 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    16861 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    16921 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    16981 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    17041 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    17101 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    17161 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    17221 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    17281 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    17341 ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta
    17401 aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata
    17461 cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa
    17521 tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat
    17581 gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata
    17641 aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga
    17701 gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg
    17761 attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct
    17821 ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga
    17881 gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat
    17941 aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct
    18001 tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt
    18061 atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca
    18121 atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct
    18181 acctgataac tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg
    18241 aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa
    18301 taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat
    18361 acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca
    18421 ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa
    18481 acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt
    18541 tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt
    18601 acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga
    18661 gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt
    18721 tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca
    18781 ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt
    18841 tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt
    18901 gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg
    18961 gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt
    19021 gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc
    19081 tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa
    19141 ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt gacggacgta
    19201 cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga
    19261 aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat
    19321 ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc
    19381 ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc
    19441 ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa
    19501 atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt
    19561 caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt
    19621 aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga
    19681 aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa
    19741 gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt
    19801 taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac
    19861 acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat
    19921 gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg
    19981 ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag
    20041 tgtatttgta ttatttttgc tctctcgaat atcattccta agttcttcta ggatattttc
    20101 ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac
    20161 tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg
    20221 ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa
    20281 tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat
    20341 gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga
    20401 cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat
    20461 aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg actacacaat
    20521 tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga
    20581 tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca
    20641 tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc
    20701 gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag
    20761 tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg
    20821 attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt
    20881 ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg
    20941 cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat
    21001 tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta
    21061 atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg
    21121 caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga
    21181 gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc
    21241 agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg
    21301 tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa
    21361 cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg
    21421 gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag
    21481 acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc
    21541 tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa
    21601 atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg
    21661 tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa
    21721 atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg
    21781 ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga
    21841 aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt
    21901 aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt
    21961 actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt
    22021 atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac
    22081 gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt
    22141 gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac
    22201 gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga
    22261 taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga
    22321 tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa
    22381 acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc
    22441 cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga
    22501 aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga
    22561 taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa
    22621 ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga
    22681 ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat
    22741 tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc
    22801 gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga
    22861 aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa
    22921 atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc
    22981 atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt
    23041 tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa
    23101 acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag
    23161 attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt
    23221 ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg
    23281 agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg
    23341 aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag
    23401 acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt gggcagagat
    23461 ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa
    23521 gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg
    23581 catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa
    23641 ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag
    23701 gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat
    23761 gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg
    23821 acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta
    23881 actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt
    23941 gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca
    24001 caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat
    24061 gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct
    24121 tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt
    24181 attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg
    24241 aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa
    24301 actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt
    24361 agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa
    24421 tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt
    24481 agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc
    24541 aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg
    24601 tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat
    24661 accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt
    24721 tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta
    24781 tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat
    24841 catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt
    24901 atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt
    24961 actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct
    25021 tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat
    25081 ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat
    25141 cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc
    25201 tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc
    25261 ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat
    25321 tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt
    25381 agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta
    25441 taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca
    25501 agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag
    25561 actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt
    25621 tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat
    25681 gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg
    25741 aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa
    25801 cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga
    25861 ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc
    25921 ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta
    25981 cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag
    26041 aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta
    26101 ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg
    26161 ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg
    26221 ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct
    26281 ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg
    26341 gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca
    26401 tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg
    26461 gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa
    26521 atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg
    26581 aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca
    26641 ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa
    26701 aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag
    26761 ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg cagatacaag
    26821 cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta
    26881 tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat
    26941 agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta
    27001 tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag
    27061 tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt
    27121 ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg
    27181 gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat
    27241 gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca
    27301 gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc
    27361 gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga
    27421 tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga
    27481 attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt
    27541 gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt
    27601 ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga
    27661 agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt
    27721 gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga
    27781 aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac
    27841 ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa
    27901 tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt
    27961 gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc
    28021 gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa
    28081 aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga
    28141 ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg
    28201 ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat
    28261 tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct
    28321 ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa agtg
//
LOCUS       DAZUKD010000027        27703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000027 DAZUKD010000000
VERSION     DAZUKD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.027"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..1169)
                     /locus_tag="ACG8N9_000993"
     CDS             complement(225..1169)
                     /locus_tag="ACG8N9_000993"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4896611.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1465..2409)
                     /locus_tag="ACG8N9_000994"
     CDS             complement(1465..2409)
                     /locus_tag="ACG8N9_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4896612.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2630..3844
                     /locus_tag="ACG8N9_000995"
     CDS             2630..3844
                     /locus_tag="ACG8N9_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4896613.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3866..5881
                     /locus_tag="ACG8N9_000996"
     CDS             3866..5881
                     /locus_tag="ACG8N9_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4896614.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5964..7337)
                     /locus_tag="ACG8N9_000997"
     CDS             complement(5964..7337)
                     /locus_tag="ACG8N9_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4896615.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7363..7647)
                     /locus_tag="ACG8N9_000998"
     CDS             complement(7363..7647)
                     /locus_tag="ACG8N9_000998"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4896616.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7649..9664)
                     /locus_tag="ACG8N9_000999"
     CDS             complement(7649..9664)
                     /locus_tag="ACG8N9_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4896617.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9843..10148)
                     /locus_tag="ACG8N9_001000"
     CDS             complement(9843..10148)
                     /locus_tag="ACG8N9_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4896618.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10237..11268)
                     /locus_tag="ACG8N9_001001"
     CDS             complement(10237..11268)
                     /locus_tag="ACG8N9_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4896619.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11475..12350
                     /locus_tag="ACG8N9_001002"
     CDS             11475..12350
                     /locus_tag="ACG8N9_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4896620.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12502..13344)
                     /locus_tag="ACG8N9_001003"
     CDS             complement(12502..13344)
                     /locus_tag="ACG8N9_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4896621.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13436..15313)
                     /locus_tag="ACG8N9_001004"
     CDS             complement(13436..15313)
                     /locus_tag="ACG8N9_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4896622.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15310..16731)
                     /locus_tag="ACG8N9_001005"
     CDS             complement(15310..16731)
                     /locus_tag="ACG8N9_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4896623.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16734..20123)
                     /locus_tag="ACG8N9_001006"
     CDS             complement(16734..20123)
                     /locus_tag="ACG8N9_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4896624.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20380..21771
                     /locus_tag="ACG8N9_001007"
     CDS             20380..21771
                     /locus_tag="ACG8N9_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896625.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21844..22491)
                     /locus_tag="ACG8N9_001008"
     CDS             complement(21844..22491)
                     /locus_tag="ACG8N9_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4896626.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22499..23185)
                     /locus_tag="ACG8N9_001009"
     CDS             complement(22499..23185)
                     /locus_tag="ACG8N9_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896627.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23191..24264)
                     /locus_tag="ACG8N9_001010"
     CDS             complement(23191..24264)
                     /locus_tag="ACG8N9_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896628.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24486..25160
                     /locus_tag="ACG8N9_001011"
     CDS             24486..25160
                     /locus_tag="ACG8N9_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4896629.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25165..26505
                     /locus_tag="ACG8N9_001012"
     CDS             25165..26505
                     /locus_tag="ACG8N9_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4896630.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26654..27640)
                     /locus_tag="ACG8N9_001013"
     CDS             complement(26654..27640)
                     /locus_tag="ACG8N9_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4896631.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8072 a         5240 c         5030 g         9361 t
ORIGIN      
        1 cggaaggatt ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca
       61 acagattaaa tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac
      121 cattctgttt atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca
      181 gctgttaagc agttttagga tttttagttc atattcattg tttcttataa tacagtgttc
      241 aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt
      301 gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag
      361 atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg
      421 ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct
      481 tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat
      541 tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca
      601 gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg
      661 acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc
      721 aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata
      781 tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc
      841 atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga
      901 gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat
      961 tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa
     1021 aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta
     1081 gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg
     1141 attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt
     1201 tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt
     1261 tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa
     1321 tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa
     1381 aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat
     1441 atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt
     1501 tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc
     1561 gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt
     1621 ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata
     1681 gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt
     1741 atcgatataa tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa
     1801 gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa
     1861 cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg
     1921 cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc
     1981 ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa
     2041 tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc
     2101 tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat
     2161 ctctatcctt tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc
     2221 aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat
     2281 atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc
     2341 taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc
     2401 gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc
     2461 ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt
     2521 tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata
     2581 accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa
     2641 taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc
     2701 agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt
     2761 tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt
     2821 tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat
     2881 ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg
     2941 actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta
     3001 tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc
     3061 agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt
     3121 agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga
     3181 aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc
     3241 taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc
     3301 gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa
     3361 tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca
     3421 gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt
     3481 attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat
     3541 ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta
     3601 cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc
     3661 accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt
     3721 cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag
     3781 gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa
     3841 ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg
     3901 gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg
     3961 aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa
     4021 gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta
     4081 tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa
     4141 agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg
     4201 agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc
     4261 tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa
     4321 agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg
     4381 gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac
     4441 tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct actataagca
     4501 aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc
     4561 agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa
     4621 acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga
     4681 agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat
     4741 ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga
     4801 aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa
     4861 tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac
     4921 gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt
     4981 gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat
     5041 tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa
     5101 agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga
     5161 aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga
     5221 ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa
     5281 tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca
     5341 gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata
     5401 tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca
     5461 aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga
     5521 atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa
     5581 aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg
     5641 tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt
     5701 tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa
     5761 agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag
     5821 ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata
     5881 aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa
     5941 gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt
     6001 ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc
     6061 aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca
     6121 gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag gataaataat
     6181 ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg
     6241 ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca
     6301 ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag
     6361 aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca
     6421 tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg
     6481 atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt
     6541 ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca
     6601 aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc
     6661 gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa
     6721 cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg
     6781 gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga
     6841 gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa
     6901 tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt
     6961 gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg
     7021 atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc
     7081 actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa
     7141 gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg
     7201 acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga
     7261 ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa
     7321 ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat
     7381 cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt
     7441 ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc
     7501 catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc
     7561 tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc
     7621 actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata
     7681 tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct
     7741 ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga
     7801 acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca
     7861 aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat
     7921 cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac
     7981 tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc
     8041 agttttttgg ttgatccgat aattggatca tttcttctgt taataattca gatagcatag
     8101 gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga
     8161 gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt
     8221 cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca
     8281 gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat
     8341 cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt
     8401 gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt
     8461 cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt
     8521 ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa
     8581 aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt
     8641 ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag
     8701 gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca
     8761 tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga
     8821 cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga
     8881 tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa
     8941 ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat
     9001 cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag
     9061 ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta
     9121 gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc
     9181 caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta
     9241 atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat
     9301 cttccacttc taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa
     9361 cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca
     9421 aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca
     9481 aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca
     9541 catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct
     9601 ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt
     9661 tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg
     9721 tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa
     9781 tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc
     9841 gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct
     9901 tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct
     9961 tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg
    10021 ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact
    10081 tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa
    10141 tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt
    10201 atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg
    10261 aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat
    10321 gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc
    10381 accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa
    10441 tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat
    10501 tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta
    10561 aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat
    10621 atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg
    10681 acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt
    10741 atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg
    10801 tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc
    10861 gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca
    10921 ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt
    10981 ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata
    11041 ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca
    11101 aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga
    11161 tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg
    11221 atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc
    11281 cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt
    11341 ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa
    11401 agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat
    11461 ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga
    11521 cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga
    11581 ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa
    11641 aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat
    11701 tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca
    11761 ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat
    11821 tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa
    11881 tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg
    11941 tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag
    12001 ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt
    12061 atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa
    12121 atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat
    12181 atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata
    12241 cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca
    12301 aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc
    12361 tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca
    12421 ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata
    12481 tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag
    12541 atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg
    12601 tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg
    12661 gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt
    12721 cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt
    12781 gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc
    12841 taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga
    12901 aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc
    12961 acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc
    13021 agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg
    13081 ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg
    13141 gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc
    13201 ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac
    13261 aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag
    13321 aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa
    13381 gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa
    13441 tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg
    13501 caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat
    13561 ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag
    13621 taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat
    13681 agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa
    13741 ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata
    13801 aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt
    13861 ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga
    13921 ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc
    13981 ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc
    14041 caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct
    14101 cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat
    14161 acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga
    14221 atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag
    14281 aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg
    14341 ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg
    14401 ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt
    14461 ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt
    14521 caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat
    14581 gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt
    14641 attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg
    14701 cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta ccgactgtat
    14761 ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg
    14821 cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc
    14881 cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta
    14941 aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag
    15001 tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat
    15061 aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat
    15121 cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt
    15181 tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga
    15241 agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt
    15301 tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct
    15361 tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag
    15421 aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc
    15481 ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga
    15541 aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc
    15601 gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc
    15661 gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc
    15721 taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac
    15781 agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc
    15841 cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc
    15901 agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg
    15961 tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa
    16021 aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt
    16081 tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct
    16141 aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg
    16201 atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac
    16261 catattctct gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc
    16321 tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt
    16381 gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc
    16441 gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc
    16501 tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc
    16561 tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt
    16621 gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg
    16681 tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca tatttagctg
    16741 ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct
    16801 actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga
    16861 ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga
    16921 tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt
    16981 agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa
    17041 ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct
    17101 ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt
    17161 accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc
    17221 atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa
    17281 tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca
    17341 ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc
    17401 tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc
    17461 tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga
    17521 ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt
    17581 accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct
    17641 tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct
    17701 ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt
    17761 aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc
    17821 aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa
    17881 tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta
    17941 ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg
    18001 tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata
    18061 tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact
    18121 tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga
    18181 ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg
    18241 tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca
    18301 ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag
    18361 atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga
    18421 cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt
    18481 ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg
    18541 tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct
    18601 gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga
    18661 tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc
    18721 gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca
    18781 ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa
    18841 aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa
    18901 aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat
    18961 agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa
    19021 ggtgtaatgg attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg
    19081 ttactgtagc tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg
    19141 ataccgctat ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc
    19201 cgattattgt cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg
    19261 atatcttttt gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt
    19321 gtttcttttg catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg
    19381 atatacgaat gggtccgatc attaggatct ccattccaag aatttatacc atcccattga
    19441 tttgttccgt ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa
    19501 ctggctttag gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta
    19561 gatggcacac tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt
    19621 tcgttttctt ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta
    19681 tctttcttat tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg
    19741 tttgcctggc tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta
    19801 gtgtttgatg agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct
    19861 ccttcttttc tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc
    19921 ttttcagcac tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga
    19981 tccgaaatga cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct
    20041 attgcagata atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc
    20101 agtttcttcc ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg
    20161 aatagtgaaa attattaaaa taaacaggcg ttacataatt ttatttattt taatatgata
    20221 attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt
    20281 tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa
    20341 aaacatatga tcaggttgtt ggaaggaaac tatagaaaga tggaaaaaat cattcgaatt
    20401 ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca
    20461 gaatctacat ttgtaaggta tatcgaagag atcaaaatcc gctggggaac ggatgtaacg
    20521 attcaatctt cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc agcttattta
    20581 aatgttctga tggaaatgtc gcaaagttct acaaacagtc gtttattaaa tgagattatt
    20641 gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag ccggtcttct
    20701 tttacacgta agttgaaaca atgcaatcaa attttagaaa gctatttatt aaaagtgatc
    20761 gttgaccaag gttttcagtt aactagcctt aaaagtgagc tccagttgcg tatctttgtt
    20821 acctttttct ttttaagtta ctacggaaga cgcacgttgc catttccaat cgaaaaaaag
    20881 aagatcaaag aaattatggt caagaataat tgtcagatga atttgtttca ggaagaaagc
    20941 ccatatgagc aatctttctt tattatgtat tttgttgtgc atctcatgag agaatcccaa
    21001 ggaactccat ttgaatggaa agctggagac tcttatgtac agttgataaa tatcgataaa
    21061 aaagacttct taatcttgaa aaatctctta ggaaaattga agtacagcag ctatataaaa
    21121 gtagtgaact attttgtcaa tacgattttt tcagaatttt cggagaaaga aaaaaatgaa
    21181 atcagtgagc agatacgaaa aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa
    21241 gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt
    21301 ttcccatacg atacgactca tttgacgatc cgtgcaactt actttgcaca gcagatgcaa
    21361 caccgccaga tacgtctata tgctggattt cgtgcagaac ttcaatttta ttccactcta
    21421 ctgaatcaga atctgattta ttatttatct agctatatct tttggatgct tatcggtaat
    21481 cctgaaattc accattatac ttctgataaa aaaatactga ttatcagcga cttaagtctt
    21541 cgacatagtc aatatattga agaatatatc agtgatattc ttgctgtgca taaaattcac
    21601 tctgagacaa ctgctattac tgaagatcag ctgtcgaaat acaatcttct cgagtatgat
    21661 ctgatcgtta cgaatcaacc cattttgaat gcccatgttc ctcatatatt gattgatgat
    21721 agtgtgtcat tcgcaaatga agaagaactc atccgcttat tcgagttatg aataatcaaa
    21781 aaacagctat gtataaacca ccctgataaa aatttcagga caaatacaca tagctgtttc
    21841 tttttattgt attccttcag caatccaacg agatttatag cgaaagtctt ttaacaactc
    21901 ttcttttgtc caattatttg caatgagatc cgtcaaagtg ttcattacga gggtcatgga
    21961 aaagtatagg aaactaactg cttcttccga cgattgaatg gagaaatgat tgattcttaa
    22021 taattccagc catttttcgt tcattggaga atcagccgat agttctctac gtttggttgt
    22081 ctgctgatca tttcgctggg ttaacaagta taacctttgc caatagatac tttcgtgatc
    22141 taagtcgcag caccactcta aatatttttc gatccccaaa aagaaatgtt gtttgttttc
    22201 gctaatgaat tttaagatat cttgatggac tatgtgagaa ctgcttttga tcgtatacaa
    22261 ataagcatct tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca tccccatctt
    22321 gtcaacgatt tctgagactt tgacttggct gatgtgctgt gagcaaaata tctccagtaa
    22381 taggtcatcg attctctgtt tctttgcgtt cgataaattg aaaaaagttt cttttggcat
    22441 ccacatctct cctttatctc tgattatacg tggatagccc ttcattgtca tgaaggattc
    22501 attccttctt tttctcttct tctgtcaagt acccatcttc cattcgataa acgcgatcac
    22561 tccattgaat catacggaca tcatgagtaa ccataatagt cgctttttgt ttctcatgag
    22621 cttctttaac tagtaatttg acgacttcat atgcatgatc tgtatccaaa ctagctgttg
    22681 gttcgtctgc taagatcaag cttggctcat tatataaagc gcgagcaatt gccactctct
    22741 gtctttctcc accagaaagg tctttaggat aactattttt aagtgactgg atatctaaag
    22801 attgcagcaa ggaatcgatt tttgttgaat cttctttttt tgaagtaact tggtcaacta
    22861 gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt agagctttgg agaataaatc
    22921 ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt agtgagtgac
    22981 cgttgatcac tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag
    23041 tacttttacc tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc tttacataaa
    23101 aatcaattgc tttcaaagct gagacctttg tatgaccaga accaaatgtt ttggtaactt
    23161 ttttcatcgt taatattttt ttcattttta ttaacctcct attgctgtaa ttgggtctac
    23221 tttggttatc gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc
    23281 actatatagc aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag
    23341 actagtcaaa taagcaaaca aaagagcaag cagaacgcct accaccccaa caataaagga
    23401 ctgtgctact aaagatttag cgatgaagaa atttttgatc ccttgtgctt tcattactcc
    23461 aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca cggcagcaac
    23521 cactaagaac aaaaagtata tcatcgcatt taaggtcata ttttgggcag aatagccagg
    23581 aatactttca atgaatgtcc caatagttaa taactgtaga cccttcgatg ctttttcttt
    23641 cgaaatttta gctgaatcct ttattacgat cccattgatc ggctgttctt cttcagaagc
    23701 aaacggctgt tgtccatact tcaaagcagt ccacgtatct agggacgcat agatgacggg
    23761 agaaactgtg taataagttt caggaaagat accgacaatc gtcaaaggat cgtcgtaact
    23821 gccaattttg atcgtatctc caattttata gcctgaatca gccagatttt gagagatgat
    23881 gatttcattc tttgttttaa attcatgccc tttcgtgagt tttggtaata aaaatgcttc
    23941 tttcgttgtg ccgaaaactg taacattttc tttgtttttt cctttgattg ctccgctgta
    24001 caatccgatt ggtgctttct ctcctccttc aatgtatttc agatctcctc ttgttaattg
    24061 tgaagctgca aatacttgat ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc
    24121 atcgattgct tttttaaact cttctgaaag tccattcgct agtcctgaca acatgaatac
    24181 gacatacgca atcaagaaca taataccgac gatcaagcca tatcgtaatt tagaatagcg
    24241 aatttctttg atagctagaa acatttgttt ttcctcccta tgatagattg atagtgacaa
    24301 aatgtcactt gttgaatagt gtaactctaa agtgacaatt tgtcacttgt gttgtttttt
    24361 tcttatcaat aaattgtcta agacatcttt cgataaaacc ttaacatgag tcataaaaaa
    24421 tttatttaag ccttatttat gctttccatg ataaactaaa gtgaccaaat caatgaggtg
    24481 ggaaaatgat taaactatta gtagtagaag acgataaagt attatccaat aatataaaag
    24541 aaatactcgc tgcaatcggc gagataacac aagtttatga tggtagtgaa ggactgttcg
    24601 agttggaaag tggagtttat gatcttgcaa tcttggattt gatgatgccg gaaatgaatg
    24661 gatatgaagt gttagcaaaa atacggaaag aaaaaataca gactccagta ttgattttga
    24721 ctgcaaaaga tagtttggaa gataagattg aaggatttga acgaggtgca gatgattatc
    24781 taacaaaacc tttctatcga gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct
    24841 cgctaggtct ctttgatgaa aatgttctga agtataagga tatcagttgt gatttaaaaa
    24901 gcaatgaagt cttgtataaa gaacaagttt tgcccataca aggaaaagag tttgaattgc
    24961 tcgtttattt tttacaaaat aagggaatca ttttgacaaa agagcagata tttgaccgga
    25021 tctggggatt tgattctgat acgacgatta cagttattga agtttatatg agcaacttac
    25081 gaaaacatct gcgaagtact ggattggata aagaaatcaa aaccttacgt aatgtcggtt
    25141 atatactgca aagggtgtga tgcaatgaat ggagatatta cacgaaagca acgtagtcgc
    25201 ttttttgcga caaatgtagc agcatttgct tttatctttt ttcttttggg attcattact
    25261 ttacagatat taaaccaatc tgcatacaga gaaacagatc tgtcattaga gaaaataaat
    25321 tttgattctc gaacagtaca attagagata caaagatatc agcaaggcga tcccttttta
    25381 aacccgactc aaacggaaca aaacagaaca aacatggaac agccattaat ggaaccatca
    25441 aatgaccgtt ttaacactca agtgatcttg tggtctaaaa atggtgagat attgaataaa
    25501 gcagctattg gtgggcgttt caaccaaatc aaagaattaa aaatggatac gaaaaatctg
    25561 aataccatcc aaaatattga attaaatgaa gacagtggct catctgccct ctctttccgt
    25621 tcgattacga aaaaagcgga gacgaatcaa gatggcgttg cctacatcca aattttagaa
    25681 aatacaaatc aaatcaagaa ttcattagcg acttttcgca cgattatgat tttgtgtatg
    25741 attgtttttt ggattctctc tattggtatc agttattact tgtctagtct aagcatgcgc
    25801 cctattattc tttcttggag aagacaaaaa gaatttgttg agaatgcttc tcatgaattg
    25861 cgcacaccgt taacgattat tcaaaatagt ttggaacatc tttttaccaa gcctaatcat
    25921 actattattg aagaatccga aagcatcgct caagcattgt cagagacgag gcgcctcact
    25981 ggattgactt cggatttgct gacaatcgct cggaatgatt ctgatcagca gctgctttca
    26041 aagcaaatga tcaatacaca agaatatatc gaacaacttg ttaaaccatt tcaagagatg
    26101 gccatcatag atggaaaaga gtttattctc cataacgatg caacattaga agtaccaatt
    26161 gatgaaaaaa aaatccatca agttttggtt attttgctgg ataatgcatt aaaatatacg
    26221 aatccagaag ataagatcat tttggattca tctgttaacc ataaagaatg gctgattcac
    26281 gtaaagaata ctggcccttc tatcagtgaa gaagatcaag caaggatttt tgatcgattt
    26341 tatcgggaag atcaatctcg ggcgaaagag actggcggat acgggcttgg cttggcaatt
    26401 gcaaaacagg taatcgaaca gcatcatgga cagatttctg tatccaatta tcttccacaa
    26461 ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa aataatgctt atccctaaaa
    26521 aagggctgtg acaaaaatct tgtcacagcc cttttttgtt gcgaataaac ggtgttccag
    26581 aagtagcttc ttcggaaata agccgaaatt tctttaagct gaccacttct gtcacaacct
    26641 ctttagctta cgcttacgat tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt
    26701 caatatccaa ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct tcatgttctt
    26761 ctttatgaat cccaacttct tcacgaataa ctgtatgttt gcttacatct acttgctctt
    26821 ctttgattgg gatagtaatc gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt
    26881 gatctgttac tggtttgcgt tcgatgacga tttcttcacg tttgaccggt acttctaccg
    26941 tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg
    27001 tatttacatc taatttttct tcttttagtt gaattgtatc atcatctaga tttggtttgc
    27061 gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg
    27121 gatcagttcc tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac
    27181 ctacgtccat tggatctgtt ccagtgccag tttctaatcc agtccccatt gtgcttgctg
    27241 ggctaacagt atcttgtgta tccattgtat cttctgttgt ttctggctga cgatagcctt
    27301 ttactgtgat gacaaatttt ccatcagaaa tatctcctct atattgatat aaaggatcat
    27361 tttctggtgt agccgtctca gaatcatagg tatcaaaaga aaaagtatct tttatttttt
    27421 cccataaaga acggtcttct tcaccatcca tccgattttc atctgtttca acatctatac
    27481 cagacaatcc gtcaaatcct agtctagatg ctgcttcatc agtagtataa ataacgatat
    27541 catctcgttc atacccttcg ttacgcaacc gttcaattac tgataatgct tcttccctcg
    27601 tactatagct tccgatgacc gttaaatttt tctcttccat gtaaattcct cctagcaaat
    27661 agatttatga ttggtatatt gttacttata aatttattct ata
//
LOCUS       DAZUKD010000028        25939 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000028 DAZUKD010000000
VERSION     DAZUKD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.028"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..513
                     /locus_tag="ACG8N9_001014"
     CDS             <1..513
                     /locus_tag="ACG8N9_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4896632.1"
                     /translation="EIYFKMKKYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFP
                     ETKQVLEMLKDNGGRHFILTHRLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPAS
                     LNYLIDTFHLERTDTMMIGDRRLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNE
                     ILTLQSIIKK"
     gene            569..955
                     /locus_tag="ACG8N9_001015"
     CDS             569..955
                     /locus_tag="ACG8N9_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4896633.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            1168..2559
                     /locus_tag="ACG8N9_001016"
     CDS             1168..2559
                     /locus_tag="ACG8N9_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4896634.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            3170..3538
                     /locus_tag="ACG8N9_001017"
     CDS             3170..3538
                     /locus_tag="ACG8N9_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4896635.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            3718..4659
                     /locus_tag="ACG8N9_001018"
     CDS             3718..4659
                     /locus_tag="ACG8N9_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4896636.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            4728..5459
                     /locus_tag="ACG8N9_001019"
     CDS             4728..5459
                     /locus_tag="ACG8N9_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4896637.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            5603..7783
                     /locus_tag="ACG8N9_001020"
     CDS             5603..7783
                     /locus_tag="ACG8N9_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4896638.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            7803..8285
                     /locus_tag="ACG8N9_001021"
     CDS             7803..8285
                     /locus_tag="ACG8N9_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4896639.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            8301..9062
                     /locus_tag="ACG8N9_001022"
     CDS             8301..9062
                     /locus_tag="ACG8N9_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4896640.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            9193..10527
                     /gene="murC"
                     /locus_tag="ACG8N9_001023"
     CDS             9193..10527
                     /gene="murC"
                     /locus_tag="ACG8N9_001023"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4896641.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            10834..11319
                     /locus_tag="ACG8N9_001024"
     CDS             10834..11319
                     /locus_tag="ACG8N9_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4896642.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            11312..11995
                     /locus_tag="ACG8N9_001025"
     CDS             11312..11995
                     /locus_tag="ACG8N9_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4896643.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            12121..14766
                     /gene="polA"
                     /locus_tag="ACG8N9_001026"
     CDS             12121..14766
                     /gene="polA"
                     /locus_tag="ACG8N9_001026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4896644.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            14811..15647
                     /gene="mutM"
                     /locus_tag="ACG8N9_001027"
     CDS             14811..15647
                     /gene="mutM"
                     /locus_tag="ACG8N9_001027"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4896645.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            15611..16240
                     /gene="coaE"
                     /locus_tag="ACG8N9_001028"
     CDS             15611..16240
                     /gene="coaE"
                     /locus_tag="ACG8N9_001028"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4896646.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            16292..16624
                     /locus_tag="ACG8N9_001029"
     CDS             16292..16624
                     /locus_tag="ACG8N9_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4896647.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            16936..17427
                     /gene="nrdR"
                     /locus_tag="ACG8N9_001030"
     CDS             16936..17427
                     /gene="nrdR"
                     /locus_tag="ACG8N9_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4896648.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            17450..18823
                     /locus_tag="ACG8N9_001031"
     CDS             17450..18823
                     /locus_tag="ACG8N9_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4896649.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            18823..19749
                     /gene="dnaI"
                     /locus_tag="ACG8N9_001032"
     CDS             18823..19749
                     /gene="dnaI"
                     /locus_tag="ACG8N9_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4896650.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            19878..20318
                     /locus_tag="ACG8N9_001033"
     CDS             19878..20318
                     /locus_tag="ACG8N9_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4896651.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            20462..21163
                     /locus_tag="ACG8N9_001034"
     CDS             20462..21163
                     /locus_tag="ACG8N9_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4896652.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            21160..22281
                     /locus_tag="ACG8N9_001035"
     CDS             21160..22281
                     /locus_tag="ACG8N9_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4896653.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            22296..23528
                     /gene="pepT"
                     /locus_tag="ACG8N9_001036"
     CDS             22296..23528
                     /gene="pepT"
                     /locus_tag="ACG8N9_001036"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4896654.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            23660..24568
                     /locus_tag="ACG8N9_001037"
     CDS             23660..24568
                     /locus_tag="ACG8N9_001037"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4896655.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            24603..24875
                     /locus_tag="ACG8N9_001038"
     CDS             24603..24875
                     /locus_tag="ACG8N9_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4896656.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            24886..25932
                     /locus_tag="ACG8N9_001039"
     CDS             24886..25932
                     /locus_tag="ACG8N9_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4896657.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         8668 a         4512 c         5680 g         7079 t
ORIGIN      
        1 gaaatctact tcaaaatgaa aaagtattct actagttatt tgatcaatga atcaaacttg
       61 aatgccagag agtttaatga attatttcac cgatatgaaa aagaaagtac ggaagtttcc
      121 cgtccatttc cagaaacgaa acaagtactg gaaatgctaa aagacaacgg cggtcgtcat
      181 ttcatcctga cccaccgttt gacagaatca acatggggcc tgttaaaaga acatcgattg
      241 gcacatctga ttgaggaagt agtagggatc gatcaggact ttcctcgtaa acctgatcca
      301 gcatctttaa attatttgat cgatactttt catttagaaa gaacagacac gatgatgatc
      361 ggcgatcgtc gtttagatat cgaagcaggg aaaaatgcag gagtcgctac ttgtctttac
      421 gacatcgatc attttctggg tgagatcccg gcagattatg tggtaggaaa tctgaatgaa
      481 atccttacct tgcagagcat cattaaaaag tagtatcccc tttcaaagga gatatgttat
      541 aattttcata aaggagtgat aaaattatat ggaaatcaga agacaaaaag aagttgtaga
      601 agcatttcat tatgatatga gattgccgaa ccaagaagta gaaacagatt tgaaagtagg
      661 cttctcgcct ttaaaagaaa cgatcgaaaa ctatccgaaa gaacatagca tcgtaggagc
      721 acgcttagag ttccgtttag tgtttgatac atatgtttta tctggaagtg tgagtcagat
      781 caatcatatc ctcaatcgga aaattgaagg tcaaaatgat gtcacacaag aagaagtgga
      841 cgagctagta gcaccgttat tcgatatcgt acgccgaatg tcttatgagg tgacagagat
      901 cgccttagat cagcctgggg taaaattgaa ttttcaatca gaaaaacaag aatagacagt
      961 aaaaacatac ctgcgataga ttaaagctat cacaggtatg tttttttatt actaattgta
     1021 agcaaaaaga atgtcatttc gataaaaatc gtctaaaata taaaagtcac tgaaaaaaat
     1081 tttttcagga ggatggttga agaatcattc tcgggaacct ttacttcctt cgatggactt
     1141 gtcattaata atatgaggag gagaaaaatg acaacactca tcacagtttt agtggttcta
     1201 gtttttgcgg cattattata tgtgttctat cgtatgcaaa agcgccatac aaaattttca
     1261 acacgcgtct ttacagcatt aggagttggt atcgtctttg gcggtctgct tcaattgatc
     1321 tttggtgtag acgacaaagt cgttactcaa tcaatggaat ggatcgggat tgtaggtcaa
     1381 ggatatgtat cattcttgca aatgctagtt atgcctttag tgtttatttc gatcattggt
     1441 gcattcacaa aaatgaaagt ttctgaaaag ctagggaaaa ttagtgcgac tgtattgact
     1501 tctttacttg gaacaacagc tattgcagca ttgatcggaa ttggttcaac aatgctgttc
     1561 ggactccaag gagctaaatt cacacaagga gctgcagaaa cagcacgaat ctctgaactg
     1621 gcaacacgtc aggaaacagt tgaaaactta tcgattccac agcagatttt atcctttatc
     1681 cctaaaaacg tttttgctga tttagcaggt agtcgtgcaa caagtacgat tggagtcgtt
     1741 attttcgcgg catttgtcgg aatcgcttat ctaggcgtga aaagaaaagc gccaaaagaa
     1801 gcagcattct tttcagattt agtcgacagt ctgtataaaa tcattatgcg gatcgtgacg
     1861 cttgttttgc gtttgacacc ttatggagta tttgctttga tgacaaacgt tttggcaaca
     1921 agtgattttg cagctatcgt caatttaggt aaatttgtcg ttgcttctta cgtagcattg
     1981 cttgctgttt tgattgtcca ctcactgatt ttgattggtg caaaagtcaa tccaatcaac
     2041 tatttcaaaa aagcctttcc ggtattgagc tttgcgttta cttcacgttc aagtgctgga
     2101 gctttgccat tgaatatcga gactcagaca aaagctttag gagtagatga tgcaacagct
     2161 aatttctcag caagttttgg tttgtctatt ggacaaaacg gctgtgctgg cgtttatcca
     2221 gctatgttgg caacgatcgt cgcaccagca gctggagtga atgtctttag tttggaattt
     2281 attttgatgc ttgtggcaat tgtaacaatc agttcttttg gtgtagctgg agtcggtggg
     2341 ggcgcaacat ttgcttcact gatcgtttta ggggcaatga acttgccagt agcaatcgtt
     2401 gggttagtta tctctgttga accattaatc gatatggcac gtacagcagt aaacgtcagc
     2461 ggtagcatgg tagctggtat cgtgacaagt gcacgcatca aagatttaga tcgcgatgtc
     2521 atcaatgatg attctaaagt gatcgatgtg aatgcatagt caaaaagagg ttgtgataga
     2581 agtggtcagc tttaagaaat aagaagaagt ttcctaaaaa tagtttctca aatttttgtg
     2641 aaatttcggc ttatttccga gaagctactt ctgaaacacc gtgaataaga ggttgtgaaa
     2701 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttacg
     2761 tttggttgag cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt
     2821 gagcctgccg tttcactctg agtcacaaag agcaatttca aaaaatagct tctcatgttt
     2881 gttgaatatt gtgtcttgtg accgaggagt tgtcaggaga acaccgttta ccagaggttg
     2941 tgacttgttt ttagtcacga cctctttttt tatttattcc atataaatac acattattta
     3001 ttccgaaaag tgaaagcgca aataacaaaa acaaccaact tatttattat taaagaaaag
     3061 cgtatttaag taggacgatt ctgttgagag ctttcgtaga ttttcccatt ttcctatggt
     3121 aaactgacaa agttgagaat gttagaacag atcgtttagg aggaaacaga tgtttacgaa
     3181 tgaaataaaa atcatgcttt atgtggacga tgtagaaaaa aatgcaacgt tttggagagc
     3241 agttggcttt gcggaaaaag atcgtcaaga aatggatggg acgcttataa ttgaagtagg
     3301 agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg agtcccattc
     3361 acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata tctttggact
     3421 atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc ttggagaaga
     3481 atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag gtaaataata
     3541 gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt aattcaaata
     3601 ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa ataaatcgtc
     3661 aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa gaaatacatg
     3721 gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga ggaatttgcc
     3781 aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct agtagctgaa
     3841 gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta tatgaaagca
     3901 atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag cgaaggacat
     3961 gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa gctagaagag
     4021 gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt taaagaagct
     4081 attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata tgacttggga
     4141 cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc agactggatc
     4201 cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac tggtggcatt
     4261 cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt cggacagctg
     4321 catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt atgggacggc
     4381 aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga taaacaaatc
     4441 gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg tagcaatcct
     4501 gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt aggagcatgt
     4561 agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg aatctctcca
     4621 gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt tttgattgtc
     4681 gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg aaatcattga
     4741 tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt attcactatt
     4801 atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga ggtcctcgta
     4861 caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc atttacctcc
     4921 ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac tggtatgcgc
     4981 cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca ggggcatcgg
     5041 aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca gaactgatta
     5101 aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt gtccatgccg
     5161 gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt atttggataa
     5221 gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt ggacatacga
     5281 ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag gatcataaaa
     5341 tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt tttgacaagg
     5401 aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat ggaatctgac
     5461 taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt tatagagaat
     5521 attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa gaagcaattt
     5581 ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca ttcagcttgt
     5641 acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta atttgttaaa
     5701 tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg gaagtcttga
     5761 tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact tagaaaaacg
     5821 taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg agttagaagc
     5881 agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc catacaaaca
     5941 aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac tcgcgaagtg
     6001 gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata ttgacaaaga
     6061 aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta ttgctgaaca
     6121 agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata acaaaggtat
     6181 gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg aaatgtatta
     6241 tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta ctaatcgtgg
     6301 agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa ttttagctta
     6361 cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg tcagagaagc
     6421 ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac tgcgcaatga
     6481 attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc tccgtaatct
     6541 tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg cttatcgtac
     6601 tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg aagttattta
     6661 tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt ttatccaatt
     6721 aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca gtcgggaatc
     6781 tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt acgctatcgt
     6841 caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag aatttccgca
     6901 tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag atcctttagc
     6961 tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac atgacgtttc
     7021 tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta accaagtagg
     7081 tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat tgaataaaac
     7141 aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg cacgaactca
     7201 attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg gattcttgcg
     7261 agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa gttattctat
     7321 tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta cagaagaagc
     7381 agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat cagtcggaga
     7441 agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata tgcgtgatga
     7501 gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga aaccagggat
     7561 ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg atatcggtgt
     7621 gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca agcatcctac
     7681 agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg atacgaaaaa
     7741 aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag gagtaacaaa
     7801 aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa agcttccagc
     7861 ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc gggcgctatc
     7921 atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca gacgaagaga
     7981 cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta gctggaagag
     8041 ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga gggttgcgct
     8101 atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc caagattgtt
     8161 ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt ggaagatgtc
     8221 atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca caaaaaatca
     8281 gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa gactggaatc
     8341 agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg acggcaccta
     8401 cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca caaatgtatt
     8461 taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg actagaatcc
     8521 tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt gaaagtgaaa
     8581 tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt ctgatagaat
     8641 cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt cattccttag
     8701 gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc aggactcgag
     8761 caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct gtgccatttg
     8821 tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa ctgaatcatc
     8881 aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt ctccatgacg
     8941 atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat atatcagagg
     9001 aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt tctcgtactt
     9061 gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt ttcaatcgtt
     9121 tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc tagataagag
     9181 aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat caaaggttca
     9241 ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca aggatcagac
     9301 attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac gattctgcca
     9361 tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc atttcctgat
     9421 tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg ctatcacgac
     9481 tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc tcacggaaaa
     9541 acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac aagttatcta
     9601 attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt tgaggcctgt
     9661 gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac caatatcgat
     9721 tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt ccaaacaatg
     9781 gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt acgcaaatta
     9841 aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat ccaagcacga
     9901 aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga tgaatttgtt
     9961 gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt aggtgttatt
    10021 gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat gctgactttc
    10081 ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt agtcgatgat
    10141 tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca aaaatatcca
    10201 gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat tgcattgatg
    10261 gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga tatttttggt
    10321 tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa aatcaaaaaa
    10381 ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca tgatgcagta
    10441 gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga aaaactttta
    10501 agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat aaaaatagaa
    10561 agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg ataaacggtg
    10621 ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga gaagctgttt
    10681 tttcttggct gttcttatta tttgatgcag gacagctttt tcacgacctc tgtaaactat
    10741 aggaatctgt taaatcaagg gagcagaact tgccttttac gctacttttg gtacaataat
    10801 gtatgattaa aaataaactg gagggtttct atcttgaaca taggtaagcc gagaaaacta
    10861 ggaaaaacaa taaatgaaat cgaggaaggc gactcgttat ccttaacaga atctattgaa
    10921 gataaccagc ttttgctgta tttgggattg acgaatgatg caaatccttt gtatatccaa
    10981 catgactttg cacaaaaaac agaatatcaa cagccaatcg ttccatcagt gatgctgatg
    11041 gggatcatca caagtgcaat ttcaaagcat cttcctggac caggatcaca tgttgtcaat
    11101 ttttctgtca attttattga accggttttc cattatgaaa cactgacttt tcagtttgag
    11161 gtcataaaag tagataagat gaaggatgtg gtgacgattt ctgtagaagc ggagaatacg
    11221 aacgaagatc gtgtgttgga tgctgtcgtt atggtccaac cgccacaagt tcctgaattg
    11281 gaaatagcaa tcgaaagtga aggtgaattg aatgaataat ccaagtacat caatgtcagg
    11341 tatcaatcgt ttttatgcaa aagtttatgg atttttaggt atgggacttg cccttagtgc
    11401 agtcacttct tacttggctc tttgggtatt tcctttgcaa ttagcaactt ttatcaataa
    11461 ttttccacta ggctttgtgg gtctgtggat cgttgaactg attttagtaa tggttctagg
    11521 cgtaaaagca caaaagaatc cgagcttagc aatcggtgga tttattgcct actctctttt
    11581 gaatggcata acattagcgg ttactcttgc atactacgat attggaacag taacgcaagc
    11641 tttcttgaca gctactttga tgttcatggg gatgtctgtt gtcggagtaa caacaaaaag
    11701 agatcttagc ggggttggaa gagcaggaca ggcagcttta atcggagtga tcattgcact
    11761 agtcttgaac atcttcattt tgagaagtag cgtagttgag ctgttcatct ccctactgtt
    11821 agtcgtcatc tttgcaggga ttacagctta tgataaccaa agcatccgta atatctatgt
    11881 ccagacaaac ggaacagcac atagtggaat tgctgtattc atggcattga agctgtattt
    11941 ggactttatc aacttgttct tggcgtttct gagaatcttt ggacggaaca actaaaaaag
    12001 aaaaaaattc atcagttggg gctataccga attatttggt atggtcccgc tttttctttt
    12061 cgcattccgc aggattcttt gttagaatga ataagaactt tacagtaagg agttaagaag
    12121 atgacaaaaa ataaattatt actcgttgat gggaacagtg tggcttttcg cgcattcttt
    12181 gcgcttcaca attctcttga acgtttcaaa aatagaaacg gtcttcatac aaatgcaatc
    12241 tatgcattca atacgatgtt tgaaaatgtt atgcaaaaag agcaaccgac tcatgttctg
    12301 gttgcgttcg atgcaggaaa aacgactttt cggacagaaa tgtatgcgga gtacaaaggt
    12361 ggaagatcca aaacacctgg cgaattcaaa gaacaaatgc catatatacg tgatttgttg
    12421 acaggactag gggtacagta ttacgaattg cccaattatg aagcagatga tattatcggt
    12481 actttggcag aaaaagtaga caaagatcaa tttgatgtcg tcgtgctttc aggtgatcgg
    12541 gatttgactc aattagctag taaagaagta aaagtggata tcactgtaaa aggtgtcagc
    12601 gatatcgaaa gctatacacc agaacatgta gcggaaaaat atgatgggtt gacgccaaaa
    12661 cagattattg acatgaaggg actagccgga gatgcctctg acaatatccc cggtgtgact
    12721 aaaatcggtg aaaaaacagc tatcaagcta ttgaaacaat atggctcagt cgaaggaatt
    12781 tatgaacata tcgatgaaat gaagcaaagc aaaatgaaag aaaatctgat aaatgacaaa
    12841 gagcaggctt tcttatcgaa aaaattggcg accatcaata cttcagcgcc tgtggatgtc
    12901 tctatcgact cattgaagta tgaagggaaa aatctcgata aactcgttcc gttttataaa
    12961 gaaatggatt tcaatcagtt cctttctaaa ttgaatatcg tagaagaaga agtaaaaatg
    13021 gatgatattt tgtttgaagt cgttcatgaa ttcaaagagg aaatgtttac tacggatatg
    13081 gcgttgtacg tggaaatgat gggcgacaat tatcatacag aggaaatcgt cggagtagcg
    13141 tgggggacag aaaagaaaat ttatgtcacg aacgatctat ctgtattcga aaaccgagcg
    13201 ttccattctt ggatcactga tccgactcgg ctgaaaaaag tatatgatgc gaaacgaact
    13261 tatgttgctt taaaccgcta tactggaaaa acgaaaggaa tcgattttga tgtcttgcta
    13321 ggtgcatacc ttctcgatac gaatgaaaaa agcacagata tcgaaggaat agctgctcat
    13381 tacggataca acgatatcca atcagatgaa gctgtatacg gcaaaggggc gaaaaaaggt
    13441 ttaccagaag aagaggaact cttttttgct catttagcgc gaaaagtagc tgcaatcaat
    13501 gcgttgacac caaaattgag ccaagaactg gcagaaaaaa accaagaaga tcttttccgg
    13561 aaaatggaac tgccattgtc tatcattttg gcagagatgg aaataagcgg gatcaaagta
    13621 gatgcgactc gcttacaaga aatgaagggc gagttctctg ctcgcttaag agagatcgaa
    13681 caaaaaatct acgaagaagc cggcgaagaa ttcaatttga attcaccaaa gcaattagga
    13741 gtgatcttat ttgaaaaaat gggattaccc gttatcaaaa aaacaaaaac tggatattcc
    13801 acagccgtag atgtactgga acagcttcgt gaacaagcac caatcgtaga ggatatcctc
    13861 acctatcgtc aaattgcaaa aattcaatct acctatgtcg agggactgct gaaagtgatc
    13921 caatcagatg gaaaagtgca tacccgttat gtccagacat tgacccaaac aggacgatta
    13981 agctctgttg atcctaattt gcagaacatc ccgattcgat tagatgaagg gagaaagata
    14041 cgtcaggcat ttgttcctag agaaaaagac tggctgattt attcttctga ctattcacaa
    14101 atcgagttac gcgtacttgc gcatatctca aatgatgaac atttgaaagc tgccttccta
    14161 gaaggtcaag atatccatgc cagcacagcg atgcgtgtat ttggaatcga aaaggcagag
    14221 gatgtcacac caaatatgcg taggcaggca aaagcagtga attttggtat cgtgtatggc
    14281 atctctgatt acggactttc acaaaatcta ggaatcagcc gaaaagcagc acagcaatac
    14341 atcgatacgt actttgaaaa atacccaggt gtcaaagaat atatggaaag aatcgtacgc
    14401 gaagcaaaag accaaggcta cgtcgaaacg ctgtatcacc gccgtcgata tctgccagac
    14461 atcaattcaa gaaattacaa tatccgttct tttgcggaac gaacagctat caatacacca
    14521 atccaaggaa gtgctgctga tattttgaaa atcgcgatga tcgaattgga taaacgatta
    14581 aaagaaacag gtcttcaagc tacgatgctg ttacaagttc atgatgaact tgtcttcgag
    14641 gtaccggaaa aagaacttga atcattagat aaattggtga aggaagttat ggaacaagct
    14701 gtttctcttc atgtaccact gatcactgac agcagttggg gaaaaacgtg gtacgaagca
    14761 aaataaacat tgtttaagac agagcaatat aatgaaggtg aaaagaaaaa atgccagaat
    14821 taccagaagt agaaaccgtt cgtaaaggat tggaacggtt agtagtagga aaaacgattc
    14881 aaaaagttca ggtcttgtgg cccaagatca tcgagcaacc agagacaccc atctttgagg
    14941 cttctcttgt tggcgaaacc atccaatcca ttggacgtag agggaagttt ttaatttttc
    15001 atctcgatca ttgtgaactg atctcacact tgcgaatgga aggaaaatat caatttacga
    15061 aagaaaatac accaatcgat aaacatacgc atgtactttt cttttttgag gatggaagcc
    15121 aacttcgcta caacgatgta cgaaaatttg gtcgtatgac aatcgtcgaa aaaggagcaa
    15181 gtgcaactta ccgaggaatc atgaaattgg gacctgagcc tttgccagat agcttcttat
    15241 tagcagattt cgcaaatgga ctgaaaaagt cccacaaagc aatcaagcca ttgctgctag
    15301 accaacgtct agtgactggt ctaggtaata tttatgtaga tgaagcatta tgggaagcga
    15361 agatccatcc agaacaaccg gcgaacacac tgcgccccaa agaggtagaa caattacgtc
    15421 tatcgatcat tgatgttttg gatcgtgcaa tagaagccgg aggtaccacg attcgcagtt
    15481 atttgaatgc attaggagaa tcaggcggat ttcaagtagc actgcatgtc tatcagcaaa
    15541 ctggcaaacc atgtatacgt tgtggaacgc cgatcgtgaa aacaaaagtg gcacaaaggg
    15601 ggacgcacta ttgtccgaat tgtcaaaggc taaaaatcgc aaagtaggat ttgttttagg
    15661 tttgacgggt ggaatagcta ccggtaaaag tacggcagcc aaagtctttc agtctcatgg
    15721 atttcctttg atcgatggcg atgtgattgc cagagaaact gttgagcctg gaacgcttgc
    15781 tctgaggaaa attgtatcag tgtttggcca agaggtattg cagcctgatg ggcaactaga
    15841 tcgagggaaa cttgggatga ttgtttttcc atcaaaggaa aaacggcaaa aacttgatca
    15901 gctattagat ccgtttatcc gaaaagcgat aaaagatcaa atcgcctctc taagttccaa
    15961 tcatccttta gtaattgtag atatcccatt gctctacgaa ggtcattatg accactatat
    16021 ggatgcagta gctgtcgttt atactacgcc agaaactcag cttcagcgtt tgatgaaacg
    16081 taatcaactg acgctagaac aagcgcagca gagaattagt agtcaacttc caatcgaaga
    16141 aaaaaagcag cgtgcggata ttttgttcga caacaatgga acaaaagaaa aacttgtggg
    16201 acaaatcgaa aattggcttt ttgctaatcg ctttctttga gtatctgtgt caattttgct
    16261 atcctgagta tatagaaagt gagggatagc gatgggatca ttaaaaaatc aaaaggataa
    16321 agtcgtaggt aacgcaaaag aaaaaatcgg cgagatgctc aataaagaaa atctaatcga
    16381 tgaaggtcgt acgcaatcag aaacagcaga agaaaaagaa aagcagtacc aagcaagaat
    16441 cgatgaaata gaagaccagc aggcagaaga agaagcgaag cggcaagatt tgactcaaga
    16501 acgaaaaaat ccgacacctg atcgtagtaa aacaccgctc ccagaaagtg accacattcc
    16561 cgatgagcaa aaaatcgagg aacagcgaat gaaacactat acaggaatcg aagagaagtt
    16621 ataaaatagt aaaaaatcga gatgtgactt gaagcagatg agaaggtgtt catctgcttc
    16681 aagttttttg tgctagatga ctaaatcagc ttttgtgtaa gttatttctc agtaatcaag
    16741 gtgatcagta ttggaaaatt gaaatttagt aaggacaggg gacacaatta cgcatgaaag
    16801 tagaaaaaat tgttcttata tccgtgtttt ttgggtagga cagtcgcgtt tgtgatttgt
    16861 ttcgtgttat aatacaacta gttgtaaaga aagacacatc ctttatataa aatgcgatgc
    16921 aagaggtgag acaaaatgag atgtcccaga tgtcaacata ataattcacg agtaatcgac
    16981 agccgtcaag cagatgatgg acgtgcgatc cgtcgccgtc gcgaatgtga gaattgttcg
    17041 ttccgattca ctacatttga acgaattgaa gctgctccgt tattagtaat caagaaaaac
    17101 ggggaacgcg aagagttcaa tcgcgataaa attttaagag gactcatccg ttcagcagaa
    17161 aaaagacctg tagctatgga acaaatggaa caaatcgtca accatgtcga aaaccgcgta
    17221 cgcagtctcg gagaaaacga agtatcgacg aatctaattg gtgaatatgt gatggaagat
    17281 ttagttgatt tagacgaaat cgcttatatt cgttttgcaa gtgtttatcg ccagttcaaa
    17341 gatatgtctg ttttcttaaa agagttgcaa gatatcgtgg ataaagcaaa agaaaataat
    17401 aaagagaata ataaagaaaa caattaagaa acaaaaagga ggcatttcct tggaaaatgc
    17461 ttggaaagag cttcaaccaa aaaatattta ccaaggaaga aaagcggctc cgctgagaga
    17521 agacagcaag aacgccttgc tgtatcttta tcagccacta atcggcggag aagccttgag
    17581 tatctatttc accctgttat cagaaatctc tcaagaatct ggcaaaggtc ctgaagggat
    17641 gcatgcagat ctgcttagta gtctaggctg cggtatcccg caattttatg atgcaagaaa
    17701 aaagctagaa ggaatcggct tactggatgt ttattttaaa gaagatagtg atctgggacc
    17761 tcgttttatt tatgagctgt tagaaccact cattcctaaa gcatttttca aagactctgt
    17821 tctggctttt cttcttctgg aaaaagttgg tgaacgacga ttccagaagc ttacccaatt
    17881 ctttgaacct aaaaggcata cagcagaggg atatcaaaaa atcacaaaaa aatttttaga
    17941 tgtttatcgc ttcaatgaag cagtgtacgc tgctaatcaa gaaaacatcg aatcgctgaa
    18001 taaatctttt gaagaatctg aatcagtagc attacttgaa gcagattcga cattcgattg
    18061 gcagtttttg aatgattggc tggcaaaaaa acatattgca agtcttgatg atccaatcat
    18121 caaacaagtc gacatgtatc atcagcttta cggtttcgat gaattgacac ttggcgaact
    18181 gatcagtcaa tcttatgact ttagtgaaca aaaagtatct gaaaaagaac tgcaaaaagc
    18241 cattttggca atgcagagac gtccggtgaa gcaaacaaaa gaaacagttg cggtttctga
    18301 aaacagtaat gaaccctcgt caaacgaaca tttgacaaac gcagcgcttc agctgataaa
    18361 agaagcacag agcattccac cgatgaaata tctggaagca atcaagtcag aaaaaggcgg
    18421 atatgtttct aaacaagaaa actggttact gcaagatctt gtttctcaat caggtctttc
    18481 cagtagcgtg atcaatgttt tgctgaatta tgtactagtg atcaagaata atgctagctt
    18541 aaatgccagt tatgtaaata cagtggcaaa tgaatgggca caaaagaaaa tcgccacagc
    18601 agaagaagcg atccagcaca ttcggcaaat aagtaatcaa gcaaaacaat caaaacaaaa
    18661 gaagcaaaca aattatcaaa ctggcaagcg aaatgttcga cgggaaaagc ttcctgattg
    18721 ggtcaaccag ccaaaagatg aaaaacagat cagcagcgag aaaaaagcag agattgaccg
    18781 ccgttttaaa gaatacctgg caaaaaagga gggtgatatc tagtggaaga cgtaggcaaa
    18841 gaattgaaga ggatcatcag caaacgcaat tatcaagaaa agtatgctga aatgatcgct
    18901 gaagtcttaa atgatgcgga tgttcaagct tttctaagcg cacaccaaga agaattgaca
    18961 caagcggata ttgaaaaaag ttacgctaaa ttgtatgaat tcgtgcaaga aaagcgtaaa
    19021 tatcaaaaaa acgatccaac gatgatcgct cccggctatg agccgaagct gacgttgaat
    19081 tttcattatg tggatgtcac ttatgtacca acggaagcat tgttagcacg gcaaagacaa
    19141 gaagaaatca gaagccgagt aaaagcaatg gatatgccaa aagatattca atcggcttct
    19201 tttgaaaact ttgaacgtac agatggacga ggatatgcga gcttagaagc cctagatttt
    19261 gtagagaaat atactgctga tccaaaaggg tatcataaag gactgtatct agtaggaagt
    19321 tttggtattg gaaaaacata tttattagga gcaacagcga gagaattagc gattgctgga
    19381 ttcaccacga cgctagtcca ttttccgaca tttgccgttg agatgaaaca ggcaatcgga
    19441 aaagatcaag tggcagaaaa gttagatgca gtgaaacgtt cacctatttt gatgatcgat
    19501 gacattggtg cagatgcgat gagtagttgg atacgagacg aagtattcgg tgttatcctt
    19561 caatatcgga tgcaggaaca gttaccaacc ttcttctcat cgaattttac aatggatgaa
    19621 ttggaacagc atttgtccgt cactcaacgt ggagatgaag agccgctaaa agcgaaacgg
    19681 ataatggaac gcatacgtta tttgacaaaa gaaatcgaaa tgactggtcg caaccgacgc
    19741 aatggttgaa cagttgacaa gtccaactga catttgacct agtagtaaaa gcaggtcagc
    19801 gaatcattat atggacagaa atgaccggag acaatcaccg caacaagtaa tgcagaaact
    19861 taaacggagg attttttatg agggctattt tttctagtat atctgtagtt ggtctatttt
    19921 ttcttttagc aggctgcggt agcgcagata gcaacacagg ttctgttgat tcgattgatg
    19981 cgggtcaaac agtaaaaaca agcagtacga aaacggagag acaaagcata tttgtcggaa
    20041 cgattgaaag tatcactcaa gtagaagcag aagaagaaac gcttcagttg aaactcgtcg
    20101 atgtcatggc caaagaagat ccagcgaata tgggaacgtc attcagcaat gacggagtga
    20161 tcttaaatgc tactgctgaa aaaataagcg tggacataaa tgaattgaaa atcggtgaca
    20221 aagtccaatt tacattagtt gagatgccga tcatgacgat gtctatccca ccgcaagttc
    20281 ctggagattc gattattgat gtttcattga acaaataacc aataacattt tttcaaaaat
    20341 aagaaaatgt tgttttatag aacgaaaagg tatatttttt aaggagatat gggaattttt
    20401 ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa ggagtgaagc
    20461 tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg tccctgaatc
    20521 cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat tgatattaag
    20581 aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg aatcagcacg
    20641 taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg caaatggtct
    20701 agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta tgggaggttc
    20761 actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg gacaagaacg
    20821 tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc aagaaaatca
    20881 gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg aaatcattgt
    20941 agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt tcgggccatt
    21001 tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc ttcaacaaag
    21061 aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc aaaaacaaat
    21121 cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag cggacgcact
    21181 ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg agacccagtc
    21241 ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac attagacgtt
    21301 cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat tgctaaacat
    21361 cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga aaaaatgtat
    21421 gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat ggatattatt
    21481 tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga gagttattta
    21541 gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt ggatcatgca
    21601 cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga ttataccggt
    21661 acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc acaaccagcg
    21721 attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt tattttgccg
    21781 gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta tgaggaacct
    21841 gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg acgagtaggt
    21901 gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga agcgttccaa
    21961 ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa gcgcattgcg
    22021 atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag agcagatgtc
    22081 tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc tgggttgatt
    22141 gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat cgagaaattt
    22201 gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga aacaaataca
    22261 aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt ttgccacgtt
    22321 ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg actccatcga
    22381 ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag ttaggcttag
    22441 ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca agcaatgttg
    22501 atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat tttaatgcag
    22561 taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc gtactagatg
    22621 cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat tatgaaggcc
    22681 acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa tcagggattg
    22741 cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct cacggtacca
    22801 tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa ttcgatgttg
    22861 agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt gagttgcagt
    22921 atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat gtacatccag
    22981 ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt cataatcagc
    23041 ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc catttgatgg
    23101 cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat catgatcgtg
    23161 aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg aatcagcgat
    23221 ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg aaagaaatca
    23281 ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag cttggtatct
    23341 ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct tacatgggta
    23401 ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc gaatttgttt
    23461 ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa ttgaacacaa
    23521 agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg atggaatgtc
    23581 atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc ttttttgaag
    23641 agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa gttcagggaa
    23701 agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa agcagccaga
    23761 attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat tagtcaagaa
    23821 agcagaagcc atagaagctg atactttagt aattataggt ggagacggca cgattcatca
    23881 tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac ttccaggtgg
    23941 aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag aagctgctga
    24001 cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg atgaagtcat
    24061 tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca gtcaagaaga
    24121 aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat tgctgaagaa
    24181 gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa aatcccaact
    24241 gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg actcagcgac
    24301 cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt tccgatttat
    24361 tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca ttacttattt
    24421 cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc gaacggatgg
    24481 agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat taacagttta
    24541 ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt ggaagaagag
    24601 taatggatgt attgttacac aatcctttgt ggttttacct tttcattgtg aatttttact
    24661 tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg cgtgttcctg
    24721 agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg atctctcgaa
    24781 aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct gcaggtgtac
    24841 ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc gtctaaagga
    24901 acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc cattatttat
    24961 tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac cagtgttgtt
    25021 gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg gcgctcaatc
    25081 aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt cttgacctct
    25141 agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat ctcggagatt
    25201 tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac agcactccat
    25261 atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt catccaattc
    25321 tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg tatccttttg
    25381 acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt tcaaatattt
    25441 tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg ggtggataca
    25501 tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca agacaggact
    25561 gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta tgtacttcct
    25621 tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat ttcgtttatc
    25681 agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg ttcctttgaa
    25741 tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt ttcttcgctt
    25801 ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt gtatgtgatt
    25861 gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga gaataaagaa
    25921 aatcaagggt aggtctttt
//
LOCUS       DAZUKD010000029        25532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000029 DAZUKD010000000
VERSION     DAZUKD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.029"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..2196
                     /locus_tag="ACG8N9_001040"
     CDS             136..2196
                     /locus_tag="ACG8N9_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS4896658.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2279..2848
                     /locus_tag="ACG8N9_001041"
     CDS             2279..2848
                     /locus_tag="ACG8N9_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896659.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3023..3829)
                     /locus_tag="ACG8N9_001042"
     CDS             complement(3023..3829)
                     /locus_tag="ACG8N9_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896660.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3967..5481
                     /locus_tag="ACG8N9_001043"
     CDS             3967..5481
                     /locus_tag="ACG8N9_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4896661.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5884..6867)
                     /locus_tag="ACG8N9_001044"
     CDS             complement(5884..6867)
                     /locus_tag="ACG8N9_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4896662.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6872..7519)
                     /locus_tag="ACG8N9_001045"
     CDS             complement(6872..7519)
                     /locus_tag="ACG8N9_001045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4896663.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7604..8236)
                     /locus_tag="ACG8N9_001046"
     CDS             complement(7604..8236)
                     /locus_tag="ACG8N9_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4896664.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8252..8803)
                     /gene="coaC"
                     /locus_tag="ACG8N9_001047"
     CDS             complement(8252..8803)
                     /gene="coaC"
                     /locus_tag="ACG8N9_001047"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4896665.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8800..9537)
                     /locus_tag="ACG8N9_001048"
     CDS             complement(8800..9537)
                     /locus_tag="ACG8N9_001048"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4896666.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9534..10523)
                     /locus_tag="ACG8N9_001049"
     CDS             complement(9534..10523)
                     /locus_tag="ACG8N9_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4896667.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10781..11323
                     /locus_tag="ACG8N9_001050"
     CDS             10781..11323
                     /locus_tag="ACG8N9_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4896668.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11340..12425
                     /locus_tag="ACG8N9_001051"
     CDS             11340..12425
                     /locus_tag="ACG8N9_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896669.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12437..13243
                     /locus_tag="ACG8N9_001052"
     CDS             12437..13243
                     /locus_tag="ACG8N9_001052"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896670.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13244..14071
                     /locus_tag="ACG8N9_001053"
     CDS             13244..14071
                     /locus_tag="ACG8N9_001053"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896671.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14068..15141
                     /locus_tag="ACG8N9_001054"
     CDS             14068..15141
                     /locus_tag="ACG8N9_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4896672.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15569..18265
                     /locus_tag="ACG8N9_001055"
     CDS             15569..18265
                     /locus_tag="ACG8N9_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4896673.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18330..18830
                     /locus_tag="ACG8N9_001056"
     CDS             18330..18830
                     /locus_tag="ACG8N9_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4896674.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18842..19336
                     /locus_tag="ACG8N9_001057"
     CDS             18842..19336
                     /locus_tag="ACG8N9_001057"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4896675.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19357..20166
                     /locus_tag="ACG8N9_001058"
     CDS             19357..20166
                     /locus_tag="ACG8N9_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4896676.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20163..20984
                     /locus_tag="ACG8N9_001059"
     CDS             20163..20984
                     /locus_tag="ACG8N9_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4896677.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21035..23134
                     /locus_tag="ACG8N9_001060"
     CDS             21035..23134
                     /locus_tag="ACG8N9_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4896678.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23131..24249
                     /locus_tag="ACG8N9_001061"
     CDS             23131..24249
                     /locus_tag="ACG8N9_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4896679.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24288..25166
                     /locus_tag="ACG8N9_001062"
     CDS             24288..25166
                     /locus_tag="ACG8N9_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4896680.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25312..25509
                     /locus_tag="ACG8N9_001063"
     CDS             25312..25509
                     /locus_tag="ACG8N9_001063"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4896681.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8064 a         4449 c         5408 g         7611 t
ORIGIN      
        1 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
       61 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
      121 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
      181 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
      241 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
      301 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
      361 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
      421 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
      481 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
      541 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
      601 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
      661 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
      721 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
      781 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
      841 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
      901 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
      961 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     1021 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     1081 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     1141 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     1201 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     1261 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     1321 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     1381 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     1441 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     1501 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     1561 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     1621 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     1681 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     1741 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     1801 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     1861 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     1921 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     1981 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     2041 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     2101 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     2161 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     2221 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     2281 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     2341 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     2401 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     2461 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     2521 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     2581 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     2641 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     2701 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     2761 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     2821 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     2881 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     2941 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     3001 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     3061 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     3121 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     3181 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     3241 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     3301 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     3361 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     3421 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     3481 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     3541 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     3601 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     3661 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     3721 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     3781 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     3841 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     3901 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     3961 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     4021 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     4081 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     4141 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     4201 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     4261 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     4321 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     4381 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     4441 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     4501 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     4561 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     4621 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     4681 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     4741 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     4801 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     4861 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     4921 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     4981 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     5041 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
     5101 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
     5161 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
     5221 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
     5281 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
     5341 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
     5401 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
     5461 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
     5521 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
     5581 ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
     5641 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga aaaagagcgt
     5701 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     5761 gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
     5821 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt gatcagcatg
     5881 attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt ccatatattc
     5941 atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc gaatcttgcc
     6001 attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg ctttatactg
     6061 atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa tcaatacacc
     6121 aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc aacgtccatc
     6181 aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat ccaaaataac
     6241 atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt gttttttata
     6301 gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt ctgatcctgc
     6361 agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg ctgttcccac
     6421 accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc caagccagta
     6481 aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa catagtctag
     6541 cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg cataaccacc
     6601 tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat cacttttatt
     6661 ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt tcaaagagac
     6721 tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt tgatcagtaa
     6781 ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat ctacagaacc
     6841 aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat tcttggaatg
     6901 ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa atatccgttt
     6961 cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct
     7021 tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc aatgtgactg
     7081 gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca taatgtttag
     7141 taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca taaacagcac
     7201 tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt ttgattgcgc
     7261 catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa atcaatacat
     7321 cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca aaagagacat
     7381 acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc cctgcaaaaa
     7441 tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac
     7501 ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc attcaatagt
     7561 aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta gtaagagaag
     7621 ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga cctgcgacga
     7681 ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa acagcattta
     7741 aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc atattcatca
     7801 ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa acggaatctg
     7861 ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc accccaatca
     7921 agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct gttccgatga
     7981 ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg ctacggatca
     8041 tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct aaaacgatcg
     8101 aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata gggataaaac
     8161 ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt
     8221 tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta agagtcagca
     8281 tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa tctccacaag
     8341 ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat gtttccagat
     8401 tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca atcaattttg
     8461 gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca atgccgtttg
     8521 ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca gcttgttttg
     8581 ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct gtatggactg
     8641 cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg cttttagaca
     8701 taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca gcgcttttgt
     8761 aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt cctccagtct
     8821 aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt ccactgttcc
     8881 atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc
     8941 gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca gaagagattc
     9001 ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct gccattcacg
     9061 tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct cggtgttaga
     9121 agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat tgtccagttc
     9181 ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa aactaaaagc
     9241 aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt cttttttcgt
     9301 tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa tcgttatatt
     9361 ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg tggtatccgg
     9421 attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat
     9481 agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa tcttcattta
     9541 tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag tttagatgct
     9601 gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc aatctgcaaa
     9661 tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac agaatcaatt
     9721 ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag tttgattccc
     9781 ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag aagactgctt
     9841 agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc taaagctttt
     9901 gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag actttttagc
     9961 catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc tagtttatgt
    10021 aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac aactttttta
    10081 tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt aaaccctgct
    10141 gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac taaccagtct
    10201 cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag cccaaaacca
    10261 gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac gacagttccg
    10321 gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga ctcgctcatt
    10381 gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac ttcattttca
    10441 gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc ctttattacc
    10501 tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc ttttatcata
    10561 ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa
    10621 aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc agcaaaaaaa
    10681 attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt ggtagtaaac
    10741 aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg gtgagaagtt
    10801 aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac gcacagattt
    10861 gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa tggaaacatt
    10921 cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc aagaagcagc
    10981 gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg aagaaaacgg
    11041 gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct
    11101 gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg aaacgtttat
    11161 ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg tggcaaaagc
    11221 aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa
    11281 acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg ggtaaaaacg
    11341 tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat gaaacagtat
    11401 taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt ggtccttccg
    11461 gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct acagaagggg
    11521 acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga caagtaaata
    11581 cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac gttgcttttg
    11641 gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc
    11701 gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa
    11761 gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg ctattagatg
    11821 aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa ctgcgggatc
    11881 tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa gaagcgttgg
    11941 caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt ggtacacccg
    12001 tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg gagagcaata
    12061 tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag caatttgaat
    12121 gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc ccagaggatt
    12181 tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc
    12241 gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg atggtccatt
    12301 caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca gaagatatcc
    12361 atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac agctacgaag
    12421 aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt atattttgtg
    12481 gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt tttttgatat
    12541 gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga catcgctttc
    12601 tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc tgatcatcag
    12661 ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc tgatgctgat
    12721 tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg ggattttcag
    12781 tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga aacagatatt
    12841 attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat ttatgattat
    12901 gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta gtagagattt
    12961 aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat taaatggtgt
    13021 gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc tgactcgtct
    13081 gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt tcttggttac
    13141 acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta tgttcatcgt
    13201 gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg
    13261 gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct tctatctgat
    13321 tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta cttgggaaaa
    13381 ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata cgttcatgct
    13441 ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta tgggaatcta
    13501 ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca tcttattagt
    13561 ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac taggattcgg
    13621 attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa tcgtcgtctt
    13681 gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct
    13741 tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt ctccaggaat
    13801 catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg ttacattttt
    13861 cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg caagacaagg
    13921 aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca tgatattggt
    13981 cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag
    14041 agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt tttaggtatt
    14101 ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg
    14161 agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc tagtttgatc
    14221 acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga ttctaatgaa
    14281 gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat accaagtgaa
    14341 tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca ttcgaagatc
    14401 aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc agataatcag
    14461 tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa attctttgat
    14521 gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga cagtttgatg
    14581 ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg ctattctttg
    14641 aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca tttaacacca
    14701 aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga agaagcctcc
    14761 gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga acatttacat
    14821 tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat gccaaaaacc
    14881 gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc ggaaaatgcg
    14941 gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa agcgttattg
    15001 ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat ctcccatctt
    15061 gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct attcttagaa
    15121 ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta tcaacgttct
    15181 aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc aaaagcggat
    15241 gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta ccagagaaaa
    15301 gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa gattgaaaaa
    15361 gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt ctgtaatcta
    15421 ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa acataaaaat
    15481 catattagat aaaaactaaa ttggtagata aattattttg tcagatatct gaggcaaata
    15541 ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa tatagaatag acatattgca
    15601 gccgatagtt tattatgctt ttagacacat taaactggag gaaaaaatga aaaagagcgc
    15661 taaagatcga gtttttgatg agctgatacg actaacaggg aatatgtctt ctgtcactgc
    15721 cgagaaaatt tcgaagaatc taggtatctc tcgtcagaat gccagccatt atttgacgag
    15781 attagtggaa gataaaaagg tcgagaaatt accgggaaag cctgttttgt ggaagccttt
    15841 agatgaatat gcggttgtag ataatacgga gcaaataaac gaagcctttc attctgtagt
    15901 tggtcatgac ggaagtctga gagaggtcat tcaaaaatgt atagccgctg tcaaatatcc
    15961 gcctaacgga ctcagtgtat taataaatgg agccacgggt gttggaaaaa gttttttagc
    16021 cacaaaaatt tttgaatacg ctatccatga gcaaattatt gaaaaagatg caccatttgc
    16081 tatcttaaac tgtgccgatt atgcggacaa tcccgaactg ctttcggcaa cactgtttgg
    16141 atacaagaag ggggccttta cgggggctga aaaggatacc gatggtttat tggcaacggc
    16201 caacaacggc tatctttttc tcgatgaggt ccatcgccta tcgaaagaaa atcaagaaaa
    16261 gctctttcta tttattgata ctggaaacta tcgtccagtc ggtgaaaatg taaattggca
    16321 ttctgctaag gttcgtttta ttttcgccac gacggaaaaa ggtgaaaatt atttattaga
    16381 cacctttgat cggcgtattc aaattagtgt tatgttgcca acttttgaag atcgccccat
    16441 tcgagaacga ttagagctga ttcaattatt ttttcaaaat gaagcggatg ttttacaaaa
    16501 agatattatt gtttcaagag aagccttgtc tttaatgtta tctcatccct ttagtgggaa
    16561 tattggtaag ctgaagaata tcattaaaat cagttgtgcg gatgtgtata gtcgaacaca
    16621 agaagaacca ttaacgattg ggaaaaatga gatcttgatt cagttaaata tgctggaatc
    16681 cgaagtagaa agcttgccga ttgctagttt attgataaag aaaaatcaaa agataaaaac
    16741 ggatcgaatc aaaccgtttg attatgattc gattttggaa aaaattactc agctgataaa
    16801 tgaaggtaat ttcaatgaaa atttttcttc gataaagaat gaagtacagc atttggtgca
    16861 tcacattgat aaggattctt ttttatcaat gagtagaaaa ttgatgattc agatgtttag
    16921 aaaggtctgg gtatccgttt taggaaaaac gtacggattg acgacgagtg gacctgttgc
    16981 agaaattatt agcagatcct atgtcatttc cccaagctat aagacagact taaccgggat
    17041 aaatcgtaca ttagctgcaa atctacctcg gacgacttac ttatgtaatc agttcttgga
    17101 gcatttacca ccgctctcag aaaaggacga gaactttcta agactgctta ttgtcgtgac
    17161 tttatctgat tatgtagatg aatccatcga gctgaagggc ctgttgcttg ctcacggaga
    17221 tagtacggct agcagcattc aagcagtagt gaatcaatta tgcggcaatt atgtttttga
    17281 agcgttggat atgccagtag agagtaatgt gtcagagatt gtcgaatcga caaggaattt
    17341 cgtgaaacgg caagtgaata cagatagtct agtgctgatc gtagatatgg gctctttaaa
    17401 tcaaatttac acacagatca agaacgattt aaaaggggag ttacttcttc tgaataattt
    17461 gacgacttcc attgcacttg atattggctt gaagatgacg atgaatgcgc catttaaaca
    17521 gattgcggag gatgcagcag acaagtattc aatcaatgtt caatactttg aaggcttttc
    17581 tcaaagtagc aacattatca tttcctgtat gtcgggtttg gggatttcgg acaaattgaa
    17641 agatgttttt cgtcatcatt tatcggatga atccatcgaa gttttcacga aggactacag
    17701 agaactacgt gaattaatcg accaaaatga tgaaacttat tttgacaaga caaaattggt
    17761 catcacgact tcggatcttc cacgttcctt ttcaattcct aatattaatg tttatgacat
    17821 cttagacggt gatggagcag ccaatttgga gcatattttg agtcctgaaa tgaagccggc
    17881 agaatttaac cgcttgattc aggagctagt aaaattcttt tcgttggaag gaattgcgga
    17941 ccgtttgaac tttctgaacc caacgatcgt gatgagtgaa gtggaaacag tcatttctaa
    18001 atatgaaaat tactatcatt tcactttaaa tggccgtgta aaactaaatc tctatatgca
    18061 tacggcattg atgatggaac gtttattctt ggctcggaca gaaaaatctg aagaaccgaa
    18121 ttatgatttg aaaccagatg aagatgaatt ttattatgtt tctagaagta tttttcaatc
    18181 aatggagatg aaatataatt ttaaagtcaa tgcttatgag ctttcgctgc tctatgaatt
    18241 attaggaccg tatattcaaa aataaagtgt ctaaaaaagt gtctaatctg ttttttttag
    18301 gttagacact ttttttaatg tgaattaacg tgattaaacc aatgttttta aaagttggca
    18361 caggaattgc ttatataaaa gtgaacagaa agaaaggaat taaccatatg aagaaaattc
    18421 taatagcgac tcacggtcac ttagcttcag gctttcttag cagtattcaa ttactgacgg
    18481 ggaaggttca agagattacg gtgattaatg cttatgtgga tgagtgtgat tttgaacagg
    18541 agttgaatgt atttgtaaac caggtgaacg agcaggatca gatttttgtt tttaccgatt
    18601 tatttggcgg aagtgtcaat cagaagatta ccaaaacctt tttggagaaa gagattgctg
    18661 taactatttt gtctggcttt aatctgccaa ttctgctcga aatagtattg gcaaccaagg
    18721 aattaacaaa ggaggatatt caggaactgg taaataaatg ccaacaggaa ttaaagatga
    18781 ccattcttga tgaggtacat gtagtaaatg aagaggaaga ttttttttag gaggaagaaa
    18841 aatgattaaa gcagtgcgaa ttgatgaacg attaattcat ggacaagtag cgatgacttg
    18901 gacaaagagt ctaaatttaa ccgggttagt agtagcaagc gatgaggcgg ctaccaacga
    18961 tattcaaaag atgacattga agatggctgc accatcgaat gtcaaagtca ttattaaaac
    19021 agttgatgac gcgattcatt tgttaaaaga tccgcgggcg aaggacatgc gcttgatggt
    19081 tttagtccct aatgtcaaag atgcggtaaa agttgcgaaa gctttcccaa cggaaatcga
    19141 attaatgaac gtggggaatg ctgggaaaat gacaccgcca tcggaagata agaaaacctt
    19201 atcaaaggaa gtgatgttaa cgacgtcaga aattcaagac ttaaaagaat tagttgaact
    19261 ttatccagat accgtttttc agcctacacc ggcgatggaa aaacgttcag ctatctctat
    19321 tttgagtgat ttttagaaaa aggaaggaga aaaattatgt atgatgcatt tgtagtcgct
    19381 ttggccgttt ttattggagt tgctggacat gaaatgttcg ggatgtccat gctgagtcgt
    19441 ccgattgttg ttgctccgtt agcaggagcg ttgatggggg atttgcagac ggggttgatt
    19501 gtcggggcgt cactagaagc catctttatg ggtgtcgtca atatcggaat ctctagtacg
    19561 gcggagcctt ctttggctgc tgggctggct acagcgttcg caatcaaaat gggaggaaac
    19621 gtggatgcga ttattcccat tgtctttcca ctagcagtat taggattaca gttaatgaat
    19681 atgatttttt cttttgtatg tggaccgatg gctgctaaat ttttcaaatt tgcaaaatct
    19741 ggtgatcaaa aaggaatcat caatctgcac ttctttatgt ggtttgtcca ctatgcgctt
    19801 tatgctctta ttccattctt tgccgtttta tttggagcag atgttgtcaa aagcgtcttg
    19861 gataatattc cagaggtcat catgaacgga ttaacagtcg ctggaaatct gctgccggca
    19921 gttgggatgg cgatgctgtt acggatgctt tggagtaaaa atattgctgt gtggtttttc
    19981 tttggggccg ttgttatggc gtatctcgag ttaccattga ttgcaattgc cgcagttgga
    20041 acgattattg caattgttat agcccaaaga gatctacaac taaaaaatat atctgttcat
    20101 gcagttcaaa gcgatgctgc tgtaaatgca acgcttgacc aagaagagga ggatttcttt
    20161 caatgaaatt agctgatgga atttctaaag atgagaaaaa attggttcgg aagatgtttt
    20221 ggcgctcgat gactatgtat gcttcggtga atccggtgac gatgggcggc ggtggtttcg
    20281 cctattctat gatgcccttc attaatcatt tctacaaaag tgaagaagat cgagctgcgg
    20341 cgctggaacg tcatacacag tatttttcga cgaccattcc ttgcgcgtcc tttgtcatgg
    20401 ggattgcggc atcgatggag aaggaaaatt ctgaaaaagc tggttttgac gtggaaagta
    20461 tcaacgcgat caaaacgagt ttgatgggac cattggctgg aattggcgat tcattgttct
    20521 ggggagtttg gcgtgtcgtt accgcgggac tagcgatcaa cttagccaat gccggaaata
    20581 ttttggcacc aattatcttt ttagttatgt tcaatatacc taattacttg atgcgatact
    20641 atggcggcgt tttaggctat tcattaggct ctaaatatat tgaaaagctg tatgcgaacg
    20701 ggttaattga aattctgaca aaagctgcta gtatcttggg tctgatcatg gtaggtgcaa
    20761 tgacgatcag tatggtcaaa ttcaataccg ttttgagctt ttcaatgaag ggcgaagaac
    20821 taatgaatgt ccaagaaatc ctcgatagta tttttaaagg aattgtaccg ttattggtaa
    20881 cattgagctg tttcaaatta ctagataaaa aagtcggcgt catcaaaatt atttttggaa
    20941 tggtcgtctt gggaatttta ttgtcattac ttggaatcgc atagtaggaa gctgcagcta
    21001 ctaggctgca gtttttttag caaggaggga aaagatgact attaatgaag taatttattc
    21061 acccaaacac ccatttttta atgctgagtt aaacgacgga aatgtgacta cttcaaacga
    21121 atacagctac tatcggaata ttcctgatgt aaagggattg accttcgatg taagtaatca
    21181 aaagacatta gcaaaccttg atgtttgcgg gacgattcaa acgttgacct tttatcgaga
    21241 aaatttacta actgaggaaa aaccaggtgt ttgggtcaat aaacagctct ctcaaaccaa
    21301 ttcgttatcc ttaaaggttg aagtaaacgg gattgtctac catttgtcag aatcggatca
    21361 tcgtattgaa gtcgatgtga ttcaagactg tttgcctaga tatattcatc attattcgac
    21421 gtttaaggtc attgtagtat cctttgctcc catactggct aataagcgtt taagtatgct
    21481 tgttcaacag gtttacattg tgaatgaaac acacgaaagc cttcaagttt cagtgaaaga
    21541 tgtgccttcg tatcaagaga agtattcgga tcagcaaaat gtgttgattt ctcaaaaagg
    21601 aaataaggac acgatcgcac aacaggaagt ggcaagcttc gccattgctt ttgtggaccc
    21661 aaatgctttt gaagaagtaa tgacttttca ggaatctgat atcgaaaaat ggctgaagga
    21721 aacattgaac tattttgctc agctatttgg gcagctgtca atgccaaacg atagagttgt
    21781 ccatctctat cgtcgtgcac tttatcaaag cttttctagt ttcggtatga atcgacaggg
    21841 acaggtcgtc ggcagcaatt ggggcagcta tcctgctact aatcgaattt ggaataagga
    21901 tatgtattac gcatcattgc cattcaccat gtttgaaccg gagttatgtc aaaaaacggt
    21961 tctatggttt gatcaatatg gcgttaagtt tcctggttca aaatttacag gaggtatcaa
    22021 tcattctctg agtaattcgt tatccagtgc gttactggct agtctttatt ttgaacatac
    22081 tggggataca aaattctttg agcggtgtcc gcaggttcta ctgaatgctt caagaattat
    22141 tgaggacata ttagctcaac gtcggtcagg ggaaccaatg ctttttccat ctgtttggtt
    22201 atcagacgcc tttgcacttg gaaagtatca taccggcagc aatctttgtc tttggaaggc
    22261 ctgtattgga cttgcagaaa tttttgaagc cctagaacaa ctgagtctta gcaagcgata
    22321 caaaaacatt gcctgtaaac taaaggaatc aatttgtaaa caaatgacta tcgatggtcc
    22381 ttttggtgag caatttttgg agggaatcgg tgatgaggag aaaacaacct attcagtgaa
    22441 gcactatcaa aaaccgattc ttgagcaagg gttaatcttt ttaagtgatg tgattgtgga
    22501 tggaaaaatt aatttattga tgcatgatgg agaagaatca gacactacgt tgatgccttt
    22561 ttatcaattt ttagataata aagatcattt atatcagaac accatggcct tttctgctag
    22621 ttctttcaat ccaacctata gcgaggaaat caaaggcatc acttggggat tagaatccgg
    22681 cgccactttt cctggcttta tcactgtttt aatgagcgag ctgcacaacc ctcaagcttt
    22741 cgtaacccga ttagaagaac taatttgttt ggcagattta gacggttcgt ggtggtggtg
    22801 gccgtatcgc ttagaggctc aacaaggaga agtcgtgcga gactttggct gcggcaaatg
    22861 cggctgggca tcgggtcttt ttgtcagcct aatgatcagt caatatttcg gattgaaatt
    22921 caaaaaagga gtattgcaaa ttgatcctgt tgataattta acgttttctt ggaagaattt
    22981 gaagttcggt caagccttca tttcgattga atgtacgcaa tccgagttga gtatttcaaa
    23041 tttgtcagaa aagccattga aaataagtga tacgaaaaaa attcttcacg tagaaaaggg
    23101 aaaaacgatt cacattcaaa ggagaaaaag atgagtatac taaaaaatat tacccaattt
    23161 aaaaaaatcc agagtcgagc aatcactccc gaaaatccaa caggcgagaa aggtaaagct
    23221 gcgatgttgc caagcgagct aggaccaagc cgcaaaggtc gtggcgcaat tccgctgact
    23281 tctggtgaag aaactgtgat tgcggatatt actggaacgg gaataattcg tcatatgtgg
    23341 atgacgattc gtgatcgaac agaatttggc agttttgttt tacgtgattt aatcttaaga
    23401 atttattggg atgggtcgga tagccctgct gttgaaagtc ctttagggga tttcttttgt
    23461 aatgggtttg gagagcgttg cgaggtgaac tcgttgccga tagtggttaa tcctacaggg
    23521 ggctttaatt cctatttcga aatgcctttc caaacatccg ctaaaattac gatcgagaat
    23581 ttacatccga aggatatgtc ctcatttttt tatacgataa attacgcttt ggtggacgaa
    23641 ttggaagaaa atagcctata ttttcacgca aattggcatc gggaacgcat taccaaatta
    23701 caggaggatt ataagttaat tgatcgatta aagggtcaag gctattacgt gggtacttac
    23761 cttgcgctta cctgcctaga acgttattgg tggggcgaag gcgagtttaa attctacatt
    23821 gatggggata gtgagtatcc gaccgtaacc tcaacaggtt cagaagatta ttttggcggg
    23881 gcgtgggcct ttcatgagta cgatgagttc ggacgtccac atgcgaaaaa ctattcaaca
    23941 gcctttttag ggtatcctta ccaatcaaat agagatgcca caagagatcg ttttgaaacg
    24001 ggaaaattga atgccgttca tgcctttggt gacgacgcaa tgccacgaca tggtctttat
    24061 cgttggcatc ttcaagaccc catctgtttt accgaagatt tatcggtcac tttacaacaa
    24121 atcggcaacg atgatgttcg cttatttgag cggcaagacg atgttgctag tgtcgcttac
    24181 tggtattcaa cagaagcttc aggaaatact aaacgtcatt tcactgatcg agaacgcttg
    24241 ccgcgttaat gaagtgtttt tcacatattc gaagggagga acctatgatg atatatgggg
    24301 caattgaagc tggagggact aaatttgtct gtgcagtcgg tactgaggaa ttaacgattt
    24361 tggaaagagt cagttttccc acaacaactc cagttgaaac aatgaagcag gtcattgatt
    24421 tttttaaacc ttttaaggaa caattggcag gcatcggggt cggctgcttt ggtccaattg
    24481 atattcaatg caaatcaacg agtttcggtc atatcacatc aacaccaaaa cttgcttggc
    24541 aaaatttcaa ttttgtagga acgctaaagc aatatttcaa tattccgatt gaatggacaa
    24601 cagatgtcaa tgcggcttgc tatggtgaat atgttgctgg ttctggcaaa gggttagcag
    24661 gtgttgcata cttcactgtg ggaacaggta tcggaggtgg tgccttgttc aatgggactt
    24721 tcgtggaagg gttcagtcat ccggaaatgg ggcatatgtt gatcaagcga catccaaagg
    24781 acaatttttc aggaaactgt cccttccacc aagattgttt agaggggatg gcagcaggac
    24841 cagcgattga aaaacggttg ggtgtaaaag gccagaattt attggctgac gattcgtttt
    24901 ggcagattga agccttttac cttgcccaat gtgcatacaa tacaacactg atgttttcgc
    24961 cagatagaat tatttttggc ggtggtgtaa tgaaacaaga acacatgaag aaaaaagttc
    25021 aggataaatt tgttgagctt ataaatggat atgttgaaat accaccaatt gatagctaca
    25081 ttattacgcc cgaattaggt gataatgccg gaataatcgg cggattagca ttggccagaa
    25141 aagcggtaag aaataagcaa ccataattaa tgaatgtttc attgatactc atgtcaaaag
    25201 tatttaaccg agtaatcaaa tgatcaaatg aagttggata aagcgaaaat gaattcaaat
    25261 aaaaaggctc taagtcaaat cgtgttgttt tcattaaatc actgaaagga aatgcttctg
    25321 cgtttccttt tttctttttg ttgtgcgctg tataccttga ggatattcac tggtggcgta
    25381 cacttaaata gtaatatatt gtgttacttt atgtctgtgg taatagttat agttgcttac
    25441 caatgtatgt tcatttttcg aaatgttgac ttcataatcc ttggcttaat gtttgaattt
    25501 ttttggtgat agattttttc agccgtataa aa
//
LOCUS       DAZUKD010000030        25502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000030 DAZUKD010000000
VERSION     DAZUKD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.030"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8N9_001064"
     CDS             133..3660
                     /locus_tag="ACG8N9_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4896682.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8N9_001065"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8N9_001065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4896683.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8N9_001066"
     CDS             7448..7789
                     /locus_tag="ACG8N9_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4896684.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8N9_001067"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8N9_001067"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4896685.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8N9_001068"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8N9_001068"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4896686.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8N9_001069"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8N9_001069"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4896687.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8N9_001070"
     CDS             13000..13737
                     /locus_tag="ACG8N9_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4896688.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8N9_001071"
     CDS             13751..14566
                     /locus_tag="ACG8N9_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4896689.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8N9_001072"
     CDS             14579..15226
                     /locus_tag="ACG8N9_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4896690.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8N9_001073"
     CDS             15242..15898
                     /locus_tag="ACG8N9_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4896691.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8N9_001074"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8N9_001074"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4896692.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8N9_001075"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8N9_001075"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4896693.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8N9_001076"
     CDS             18240..18758
                     /locus_tag="ACG8N9_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4896694.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8N9_001077"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8N9_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4896695.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8N9_001078"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8N9_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4896696.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8N9_001079"
     CDS             20691..21743
                     /locus_tag="ACG8N9_001079"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4896697.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8N9_001080"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8N9_001080"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4896698.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8N9_001081"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8N9_001081"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4896699.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG8N9_001082"
     CDS             complement(23383..24144)
                     /locus_tag="ACG8N9_001082"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4896700.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACG8N9_001083"
     CDS             24333..25427
                     /locus_tag="ACG8N9_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4896701.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8447 a         4424 c         5384 g         7247 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DAZUKD010000031        25299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000031 DAZUKD010000000
VERSION     DAZUKD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.031"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1866
                     /locus_tag="ACG8N9_001084"
     CDS             226..1866
                     /locus_tag="ACG8N9_001084"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4896702.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2020..3342
                     /locus_tag="ACG8N9_001085"
     CDS             2020..3342
                     /locus_tag="ACG8N9_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4896703.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3404..3835)
                     /locus_tag="ACG8N9_001086"
     CDS             complement(3404..3835)
                     /locus_tag="ACG8N9_001086"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896704.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3899..4735)
                     /locus_tag="ACG8N9_001087"
     CDS             complement(3899..4735)
                     /locus_tag="ACG8N9_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4896705.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4925..5227)
                     /locus_tag="ACG8N9_001088"
     CDS             complement(4925..5227)
                     /locus_tag="ACG8N9_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4896706.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5253..5684)
                     /gene="ruvX"
                     /locus_tag="ACG8N9_001089"
     CDS             complement(5253..5684)
                     /gene="ruvX"
                     /locus_tag="ACG8N9_001089"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4896707.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5681..5947)
                     /locus_tag="ACG8N9_001090"
     CDS             complement(5681..5947)
                     /locus_tag="ACG8N9_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4896708.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6201..6926)
                     /locus_tag="ACG8N9_001091"
     CDS             complement(6201..6926)
                     /locus_tag="ACG8N9_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4896709.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7292..7362)
                     /locus_tag="ACG8N9_001092"
     tRNA            complement(7292..7362)
                     /locus_tag="ACG8N9_001092"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7328..7330),aa:Gly,seq:ccc)
     gene            7479..8231
                     /locus_tag="ACG8N9_001093"
     CDS             7479..8231
                     /locus_tag="ACG8N9_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4896710.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8247..8726
                     /locus_tag="ACG8N9_001094"
     CDS             8247..8726
                     /locus_tag="ACG8N9_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4896711.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8977..10557)
                     /locus_tag="ACG8N9_001095"
     CDS             complement(8977..10557)
                     /locus_tag="ACG8N9_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4896712.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10550..11293)
                     /locus_tag="ACG8N9_001096"
     CDS             complement(10550..11293)
                     /locus_tag="ACG8N9_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896713.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11469..12245)
                     /locus_tag="ACG8N9_001097"
     CDS             complement(11469..12245)
                     /locus_tag="ACG8N9_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4896714.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12493..13329)
                     /locus_tag="ACG8N9_001098"
     CDS             complement(12493..13329)
                     /locus_tag="ACG8N9_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4896715.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13340..14269)
                     /locus_tag="ACG8N9_001099"
     CDS             complement(13340..14269)
                     /locus_tag="ACG8N9_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4896716.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14461..14814
                     /locus_tag="ACG8N9_001100"
     CDS             14461..14814
                     /locus_tag="ACG8N9_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4896717.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14888..15544)
                     /locus_tag="ACG8N9_001101"
     CDS             complement(14888..15544)
                     /locus_tag="ACG8N9_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4896718.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15770..17704
                     /locus_tag="ACG8N9_001102"
     CDS             15770..17704
                     /locus_tag="ACG8N9_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4896719.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17790..19013)
                     /locus_tag="ACG8N9_001103"
     CDS             complement(17790..19013)
                     /locus_tag="ACG8N9_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4896720.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19007..20878)
                     /locus_tag="ACG8N9_001104"
     CDS             complement(19007..20878)
                     /locus_tag="ACG8N9_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4896721.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20899..22017)
                     /locus_tag="ACG8N9_001105"
     CDS             complement(20899..22017)
                     /locus_tag="ACG8N9_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4896722.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22100..22933)
                     /locus_tag="ACG8N9_001106"
     CDS             complement(22100..22933)
                     /locus_tag="ACG8N9_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896723.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22926..23843)
                     /locus_tag="ACG8N9_001107"
     CDS             complement(22926..23843)
                     /locus_tag="ACG8N9_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896724.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23866..24045)
                     /locus_tag="ACG8N9_001108"
     CDS             complement(23866..24045)
                     /locus_tag="ACG8N9_001108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896725.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24070..25194)
                     /locus_tag="ACG8N9_001109"
     CDS             complement(24070..25194)
                     /locus_tag="ACG8N9_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4896726.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7806 a         5043 c         4305 g         8145 t
ORIGIN      
        1 tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga gatacgcata
       61 gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg atttcttttg
      121 actctattac tcatataaat aaaatttatt atacctgctc tttttgacta tatcctcatc
      181 tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct tacagggcta
      241 aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca
      301 aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca acaatctgtc
      361 caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat cttcttaatg
      421 aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca tttagttgaa
      481 ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt
      541 ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa tatcaaagca
      601 agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac agatgcagaa
      661 ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat cggtaaattg
      721 atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt atcaatcgac
      781 gctggtacta ataacgaaga attattgaat aatccattat acttagggaa tcgtcatgct
      841 cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc agctactgaa
      901 ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc tgcaacgatc
      961 ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg cacaggaatc
     1021 gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt gaaagatcaa
     1081 acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat ccttttagat
     1141 gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat
     1201 aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat cccattcgct
     1261 cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc agttgtaaaa
     1321 gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt cactgaagaa
     1381 atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt gtctaatcca
     1441 acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg aaaagcttta
     1501 gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta ccaaatcggt
     1561 caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc ttcaacagct
     1621 acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat
     1681 gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga attctctcaa
     1741 acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc
     1801 acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa atctttagaa
     1861 gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa
     1921 aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct tctctttggc
     1981 ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc
     2041 gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat taccggcgta
     2101 agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc tttaggaaaa
     2161 ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa tttatttcat
     2221 tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc cgtttttgcg
     2281 atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt agtaaaatcc
     2341 acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc cttgattact
     2401 ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc
     2461 gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg tatgcttatc
     2521 ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc tggtggtgtt
     2581 ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac ggacgcttct
     2641 tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc tatcattggt
     2701 gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa tggtaaatta
     2761 atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc cattccacaa
     2821 ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat ctgtaactat
     2881 tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat ctgtaaaatc
     2941 gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa cttgattgtc
     3001 aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa tctaaatgta
     3061 ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt
     3121 tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga atcaactatt
     3181 actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt cttatctgca
     3241 gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg tggtgccatc
     3301 gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa aatacctgat
     3361 catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg attttcaaaa
     3421 atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta
     3481 ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc gcttcttcta
     3541 cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac acagcaagtg
     3601 ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct tgtacatagc
     3661 cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt tctggctcct
     3721 tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat cctaattgtt
     3781 ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc aacatccaaa
     3841 tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac tccttttttc
     3901 acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct tctaatgttt
     3961 gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca atcgcttgaa
     4021 aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc
     4081 caataacagc agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac
     4141 ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat
     4201 atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg
     4261 gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca catataaggt
     4321 accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag aagaacgcaa
     4381 aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa
     4441 ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca acagctccac
     4501 tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact tgtcgcacag
     4561 tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc cctgcaacag
     4621 aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc atcccggaaa
     4681 tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca atcatcttgt
     4741 atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt attatacagt
     4801 aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt agtgctaaac
     4861 gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta tcgattcttt
     4921 tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata tcccattctg
     4981 cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat
     5041 aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa agaacatagt
     5101 tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat gtttcatttc
     5161 cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa tggttatgtt
     5221 ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc tgagccgtct
     5281 aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt tcgtttcgct
     5341 cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt tagtcgttca
     5401 tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata tgacatggaa
     5461 gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac tacaaaacgg
     5521 tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc ttcttctgcc
     5581 tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc gctcacagca
     5641 acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc actccatgat
     5701 ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga cggcgaatca
     5761 aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc aagtagccga
     5821 caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca atcgctaatg
     5881 tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa
     5941 aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc tacaccctta
     6001 ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa taaattaaga
     6061 aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc tctttccata
     6121 aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat aaagttcata
     6181 aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt ctcataattg
     6241 atggtgtagc catcttgaac attgtagata aagacattga attcaatttt tttgtcttca
     6301 atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc gaaatgagga
     6361 gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat atcattttcg
     6421 tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt tttaatgtta
     6481 ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa ccattgtccg
     6541 tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc tctcttttca
     6601 gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt caatttgtct
     6661 atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt tgtaaactcc
     6721 ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg cacatccgct
     6781 acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa gatatctgaa
     6841 tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac gatccccacg
     6901 atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt ccctaaatct
     6961 taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa aaaaataaaa
     7021 ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc tctactcaat
     7081 cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt aatactcaaa
     7141 actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc atctgcggta
     7201 ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc ttattacttg
     7261 atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga acccgcgaca
     7321 tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa aacagtatac
     7381 gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat gttttgtagt
     7441 aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa gaattagatc
     7501 tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat gcggagatgt
     7561 atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc cgtttggtca
     7621 gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc atccgaatgg
     7681 aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat gatctttcac
     7741 gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg caagcaatcg
     7801 accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca gtgatcagtg
     7861 gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact ttagtcattg
     7921 gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc aaatttttat
     7981 cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta cgttttggct
     8041 taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt cctggagttc
     8101 aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga gtttctcgcg
     8161 gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt attttccagc
     8221 ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt tcttttagtt
     8281 ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca ttgatttgtt
     8341 gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa ataggcggat
     8401 ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc gatcttttct
     8461 ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt cctctagtcc
     8521 ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat cgagaagcgg
     8581 ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt cttgtattat
     8641 cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca atcatgatga
     8701 aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt gtgaaagaga
     8761 cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta accgcaggag
     8821 caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc tcacattttt
     8881 acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc tgtaaaaaag
     8941 gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt tccaaaacgt
     9001 tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga tcacaggaat
     9061 attcactaac caaaatggat aaaacaaagc caacattccg tataagacga taagaataat
     9121 actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg aacctctgtt
     9181 aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc tagcgaaata
     9241 acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag ggataaaaaa
     9301 caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta aaaaactctg
     9361 caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga ttggcaaaga
     9421 acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa aagaagtctg
     9481 attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga ttaacttatt
     9541 atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa aaatgattgg
     9601 agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta ctaattgagc
     9661 attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc tttttatttt
     9721 gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca gctgttttac
     9781 agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata aaccttgttg
     9841 agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg gatgccgatc
     9901 tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc cagcaaatgc
     9961 gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt ttttaaacac
    10021 tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa taatcaaaaa
    10081 tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat cagctcgcca
    10141 tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg tcaaagcaac
    10201 taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca aaaatacttg
    10261 caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag ttaaacctaa
    10321 cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa agttcatagc
    10381 aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat tttgagacaa
    10441 aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat tcgtgagctg
    10501 tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct tatacatacg
    10561 gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct agcgtttcat
    10621 caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca cctttcttca
    10681 aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta gaaaaaatga
    10741 ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat gctgcttgag
    10801 gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc agcaacgtgc
    10861 cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt tcattttgat
    10921 gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt ttttgtgtgt
    10981 ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat tgtaaaaaaa
    11041 tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga ttttccgtca
    11101 atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta actaaacttt
    11161 taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt tccccttgtc
    11221 ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta cttacattga
    11281 tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt acccttcgtt
    11341 attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac gactaggctg
    11401 ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga tatgagtctt
    11461 tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt tagtttatca
    11521 atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat ttcgtttact
    11581 ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc tttacataaa
    11641 tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag aatctcttca
    11701 tctttcaata ctaggcctat tttattttta taacctggca tgctcattag ataaaggatt
    11761 tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca cctttttttc
    11821 gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg gatgcttttt
    11881 ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat ctttcgatta
    11941 ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca cttttcattc
    12001 ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa atcgatgtct
    12061 aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac aaattgtata
    12121 acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct ggattcttcc
    12181 aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt
    12241 accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata caacttttca
    12301 acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca cctttgaaac
    12361 cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa tgaatacatc
    12421 atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt
    12481 tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag agactttctc
    12541 agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat agtctgattg
    12601 gatggttcct tctattttat accattcatc atttttaacc gttactcctt ccggcatatg
    12661 cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga atcggaacaa
    12721 gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat agctgatttt
    12781 tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta aatagttttc
    12841 atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat tcatttggtc
    12901 taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt gtgtctgcat
    12961 ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg cttctacgat
    13021 cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca aagctagcag
    13081 tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat cgtgctcgtg
    13141 tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta aagccaaaag
    13201 agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga tatattgatc
    13261 gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata aaatcaaaaa
    13321 ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta agggcaatca
    13381 gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc agtagatttt
    13441 tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg ccgaacagac
    13501 ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc gtaaaggcca
    13561 ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc ataattctag
    13621 ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa taacgacctg
    13681 tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg atcagctgat
    13741 tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg tgattttctg
    13801 ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag gcaagccaga
    13861 caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc atctgccaag
    13921 aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc ggagcagtca
    13981 gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga tgaacgatcg
    14041 gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag
    14101 agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa aatacttgta
    14161 aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata acgatcgata
    14221 agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg agtttcctcc
    14281 tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg
    14341 ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg actttttctg
    14401 ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg gagaataaaa
    14461 atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc atccgttgcc
    14521 gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc acgactagag
    14581 gaacttctca cacattatga tattccggca cttcatttgg atgcatttga aacaccagaa
    14641 gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa
    14701 gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct actagatgaa
    14761 ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa ataatcgttg
    14821 tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt
    14881 tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc aagaaataga
    14941 tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct gacggagcat
    15001 tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt aactgattga
    15061 ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt tgttggatct
    15121 tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg atgcgcccaa
    15181 cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt tgatggaatt
    15241 tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt
    15301 gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca taaccatacc
    15361 cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca ctatctactt
    15421 gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta tttaaaatcc
    15481 ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata atatgatctt
    15541 tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat catagcagta
    15601 tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc tttattcatt
    15661 tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact actaattgaa
    15721 aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca tgatattatt
    15781 acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa acatacattt
    15841 agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac
    15901 tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa
    15961 aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg aaacagtttg
    16021 gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct
    16081 agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt atttaaaaga
    16141 atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg aaaatgaaat
    16201 tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga aaatcgatac
    16261 tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc aacaccctga
    16321 cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt cctggcttga
    16381 gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc gttatttttt
    16441 agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc attacaaagg
    16501 taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat ggaaggctta
    16561 tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga accttgttcg
    16621 cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct
    16681 ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag aaaagccttc
    16741 tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg tcaccttatc
    16801 tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag gtccgaatgg
    16861 tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta tccgtggaaa
    16921 agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag ctgatctgca
    16981 tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc cagaaaaaga
    17041 gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc
    17101 tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga tgaacaaaga
    17161 aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca aagaagtatt
    17221 agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg accgatattt
    17281 catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca aagtctatct
    17341 aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt
    17401 ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt attaccaaag
    17461 caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg ttgaagaaga
    17521 aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa
    17581 cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg ctagacaaga
    17641 tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag aatatgaaaa
    17701 ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt tttttatttt
    17761 atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt actagtcatt
    17821 ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt taccccatta
    17881 tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc cgttgtagca
    17941 tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa gttcttttct
    18001 gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat gatcaccata
    18061 ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca ataacaaatg
    18121 gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa atcaaaatat
    18181 ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca tgcttggcct
    18241 ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct tggttttcca
    18301 tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac taaataatct
    18361 gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc agtttcttca
    18421 ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct accttgttgt
    18481 gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca aaaacgctga
    18541 agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc cgttatttta
    18601 taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt agtgataatt
    18661 tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg ttgaaataaa
    18721 ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc ttctgtccaa
    18781 ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg gaattgatac
    18841 tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg ctcgatctca
    18901 ttattccatt ctttttccat taattcttta taacattcat ttatccattt ttctggatgt
    18961 attatctctt caaacatcac tttatcaagc atttccccac cagcctttag cataatttta
    19021 gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca aaatcaattt
    19081 tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca accactcctg
    19141 ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata aaagtgtttt
    19201 cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt cgtttaatag
    19261 tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta tagttttcct
    19321 tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca aaccctgtct
    19381 catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc cagccttcaa
    19441 aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta gaggcaagta
    19501 cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt ccataggttt
    19561 tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga atccctagca
    19621 ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa aaaccaaatt
    19681 gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa tttggcccta
    19741 attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc aaattggctt
    19801 ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc gtccaatact
    19861 catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat ggagataacg
    19921 tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata ttcaggatac
    19981 ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg actaacccct
    20041 tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac caaaagcttg
    20101 aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca tcttccgcaa
    20161 aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc tggctaatgg
    20221 gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca aaaggattat
    20281 aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga taacaatttt
    20341 ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca aatttttgga
    20401 taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat tcatattcaa
    20461 aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat tttttccata
    20521 ataattgttc atgatgatat aacgctaata acattgcggg catttctacc atacgctggt
    20581 ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca gcttttattc
    20641 ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc cccgctcccc
    20701 ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct accccttgat
    20761 gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt tgatttatct
    20821 ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga tgtttcattg
    20881 tattttctcc tctttttact accaaatagc taattccgac tgttctaatc gaagtaagcc
    20941 ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt ctctatggta
    21001 tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg catagtgctt
    21061 ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc gtacagagga
    21121 aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac tggtatcaat
    21181 attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt cgattttctg
    21241 aataaaattt actgctatct tttctgctat agctagatag tacggatttt ttgtgtaacg
    21301 ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac aagaattctc
    21361 attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat caaattctgc
    21421 gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat ctgcatgctg
    21481 ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat acagtaatgg
    21541 taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc atgctactaa
    21601 atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta accctagttg
    21661 tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta agaataaaaa
    21721 acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata attgttcttg
    21781 ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct gccataaaat
    21841 accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta cgtctgtata
    21901 ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt ccgcatctat
    21961 cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt cattcatgga
    22021 tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg aactagatgg
    22081 ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc ttaaatttag
    22141 actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa attagcataa
    22201 ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg ataattaagg
    22261 gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat tgattccaac
    22321 ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc ggtacagcga
    22381 ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc atcagtgctg
    22441 taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg gcaatcgaag
    22501 ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg taaaataaag
    22561 gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg aacaacagtt
    22621 tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa ctggctgtag
    22681 ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag ggataattat
    22741 atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg aaattgattc
    22801 cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat aaatatggca
    22861 tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg ctttgtatct
    22921 tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa tataacagat
    22981 acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat ttcaaaactt
    23041 gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt ggaagtccca
    23101 aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa aactgtggcc
    23161 attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg aaatttcgat
    23221 gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag accagctaaa
    23281 aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat tgatattcgt
    23341 atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt caaaaatcca
    23401 ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc caccgttgat
    23461 gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa ctgtgctgaa
    23521 ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga tcctgccatg
    23581 ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat agctagataa
    23641 ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa agcaaacgag
    23701 ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt gaccaatcca
    23761 taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt tattgtcttg
    23821 cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat caccagttaa
    23881 ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat tttcaaaatc
    23941 acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat ctgaataagt
    24001 ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt catagtattt
    24061 ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg ccaatgagtt
    24121 agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg tcagcataaa
    24181 tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat aaaaacttga
    24241 tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca gtggagccat
    24301 gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat accagccgcc
    24361 agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac cgtagaaatg
    24421 tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg cttctttagt
    24481 attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg ctagcatgtt
    24541 gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac cagttttttc
    24601 ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt caaaatctac
    24661 acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa aatcactagc
    24721 ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt tttgatcata
    24781 aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa aagcatcttg
    24841 aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat ccggcatatc
    24901 ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg aagctacatt
    24961 cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg cagcattttc
    25021 aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg ctttttttat
    25081 cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg cacttaagct
    25141 gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt tcatgtctat
    25201 cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat taacggaatt
    25261 agtaacacaa atatttatat aaaaaaatat tttacttgt
//
LOCUS       DAZUKD010000032        24955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000032 DAZUKD010000000
VERSION     DAZUKD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.032"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..383
                     /locus_tag="ACG8N9_001110"
                     /pseudo
     CDS             115..383
                     /locus_tag="ACG8N9_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1055..4455
                     /locus_tag="ACG8N9_001111"
                     /pseudo
     CDS             1055..4455
                     /locus_tag="ACG8N9_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            complement(4808..5656)
                     /locus_tag="ACG8N9_001112"
                     /pseudo
     CDS             complement(4808..5656)
                     /locus_tag="ACG8N9_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5809..6921
                     /locus_tag="ACG8N9_001113"
     CDS             5809..6921
                     /locus_tag="ACG8N9_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4896727.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(6999..7292)
                     /locus_tag="ACG8N9_001114"
     CDS             complement(6999..7292)
                     /locus_tag="ACG8N9_001114"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4896728.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7460..7774
                     /locus_tag="ACG8N9_001115"
     CDS             7460..7774
                     /locus_tag="ACG8N9_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4896729.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7795..9774
                     /locus_tag="ACG8N9_001116"
     CDS             7795..9774
                     /locus_tag="ACG8N9_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4896730.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9787..10113
                     /locus_tag="ACG8N9_001117"
     CDS             9787..10113
                     /locus_tag="ACG8N9_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4896731.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10129..10647
                     /locus_tag="ACG8N9_001118"
     CDS             10129..10647
                     /locus_tag="ACG8N9_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896732.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10647..11999
                     /gene="celB"
                     /locus_tag="ACG8N9_001119"
     CDS             10647..11999
                     /gene="celB"
                     /locus_tag="ACG8N9_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4896733.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12083..13588)
                     /locus_tag="ACG8N9_001120"
     CDS             complement(12083..13588)
                     /locus_tag="ACG8N9_001120"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4896734.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13680..14270)
                     /locus_tag="ACG8N9_001121"
     CDS             complement(13680..14270)
                     /locus_tag="ACG8N9_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4896735.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14449..16095
                     /locus_tag="ACG8N9_001122"
     CDS             14449..16095
                     /locus_tag="ACG8N9_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4896736.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16144..16863
                     /locus_tag="ACG8N9_001123"
     CDS             16144..16863
                     /locus_tag="ACG8N9_001123"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4896737.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17342..18289
                     /locus_tag="ACG8N9_001124"
     CDS             17342..18289
                     /locus_tag="ACG8N9_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896738.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18301..19221
                     /locus_tag="ACG8N9_001125"
     CDS             18301..19221
                     /locus_tag="ACG8N9_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896739.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19262..20719
                     /locus_tag="ACG8N9_001126"
     CDS             19262..20719
                     /locus_tag="ACG8N9_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4896740.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21027..21677
                     /locus_tag="ACG8N9_001127"
     CDS             21027..21677
                     /locus_tag="ACG8N9_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4896741.1"
                     /translation="MRKYKLNVWRRRYVREGLEELFIRIWVIIKLTLYFWIYTFAGGI
                     IFGLGAAWKTVNELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYN
                     LYLSVQIKGLIFLAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSS
                     FSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21670..23394
                     /locus_tag="ACG8N9_001128"
     CDS             21670..23394
                     /locus_tag="ACG8N9_001128"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4896742.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23391..24842
                     /locus_tag="ACG8N9_001129"
     CDS             23391..24842
                     /locus_tag="ACG8N9_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4896743.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         8115 a         4214 c         5149 g         7477 t
ORIGIN      
        1 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
       61 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
      121 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
      181 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
      241 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
      301 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
      361 tgatggaaga cgatacaagt taagaaagta aaactacgcc caaataaaga gcaaaaagag
      421 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
      481 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
      541 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
      601 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
      661 aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc aacgatttag
      721 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
      781 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
      841 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
      901 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
      961 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
     1021 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
     1081 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
     1141 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
     1201 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
     1261 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
     1321 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
     1381 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
     1441 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
     1501 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
     1561 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
     1621 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
     1681 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
     1741 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
     1801 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
     1861 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
     1921 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
     1981 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
     2041 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
     2101 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
     2161 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
     2221 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
     2281 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
     2341 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
     2401 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
     2461 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
     2521 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
     2581 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
     2641 caatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
     2701 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
     2761 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
     2821 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
     2881 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
     2941 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
     3001 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
     3061 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
     3121 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
     3181 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
     3241 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtttaa ctacaggaga
     3301 agatggaaaa ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca
     3361 agcgccaata gggtatgaat tagatgcgac accaatcgag tttgagatcg aaagaagtca
     3421 gacagcggtg gtagaattaa cgaaagaaaa ccgacttaca ccaggcggtg tcgtgttgac
     3481 aaagatcgat gaccaaagcg gggaaatcct gcaggagctg tttttgagtt acagaaccga
     3541 gaaggagaaa cgctacaaac cggtctaact acaggagaag atggaaaact ggcaattgac
     3601 ggtttagcac caggagcata tcagctggta gaaactcaag cgccaacggg gtatgaatta
     3661 gatgcgacac cgatcgagtt tgagatcgaa agaagtcaga cagcggtggt agaattgacg
     3721 aaagcaaacc gacttacacc aggcggtgtc gtgttgacaa agatcgatga ccaaagcggg
     3781 gaagtcctgc aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca
     3841 ggtctaacca caggagcaga tggaaaactg gcgattgacg gcttagcacc aggagtgtat
     3901 cagctggtag aaacccaagc gccaacgggg tatgaattgg atgcgacacc aatcgagttt
     3961 gagatcgaaa gaagtcagac agcggtggta gaattgacga aagaaaatcg acttacacca
     4021 ggcggtgtcg tgttgacaaa gatcgatgac caaagcggag aagtcctgca aggagctgtt
     4081 tttgagttac aagatgccaa tggaaccgtt ctgcagtcag gtctaaccac aggagcagat
     4141 ggaaaactgg cgatagatgg cttagcacca ggagcatatc agctggtaga aactcaagcg
     4201 ccaacggggt atgaattgga tgcgacacca gtaactttca agatcgaaaa agaacaagaa
     4261 gtggcgatct ttttaacaaa agagaataga aaagttgctg atagctcaga tataagagga
     4321 aacaatccaa caagcagtgg aaataagcat cttccaaaaa ctggagaaac catcattagc
     4381 cagtttatat tgtcaatatt gggtgtgcta cttgttttca tcagtataaa atttagaaag
     4441 aaacgaaata tatgagctaa aataaaaaat tgaataagca tagtttttaa aatcaactag
     4501 tacccatcaa ttgaatggaa actaccgatt ttagaagggg tatacaaaca aatattagta
     4561 tcggagtaga gaaggttgaa cccaaccagt aaataagtaa agacaatttt gtgctgcgtt
     4621 gagaagtgaa aaataaacct ttgtagtaat actagttaat aaaaaatata caaaaacact
     4681 gcgaaaatca ttcttgagtt tcgcagtgtt ttttgcctaa tgactcaagt ttattgggta
     4741 atactttgat tttctttcta caaataatca gcgatcagtt gtaatttttt ctctagtttt
     4801 aatggctctt tgtcaataag gagaaaaaca agtcgggtct tttgtctcgg aaagcttcaa
     4861 ccaactcgtg aataaaggtt acgcctcctt gaaaggcgtg gaaaaaccaa gtaatcgatc
     4921 aatcaccaat ttcgcatttg gtagaacatg tttggtgagc tgaaagtagg tggcgttcat
     4981 gtctgtaact aagaattcta tttctttagg attagtgcag ctaaggaaat agtttgttaa
     5041 tcgaggaatt ttttgtgtag gtaaattttc tcgattaatt tttctgtctc gccatccgcg
     5101 gaaataaaac tcagtttatc ctcaacggaa acgtgagaac gaaattcaca accctcaaca
     5161 ctcaagacag aattcttttg gaagactttg gtaaatagct tctcaactct ttcaatgtgc
     5221 gaataacggt tgtcaaagat acctaacaac ttttcgaaat aaaaaataac gacacttttt
     5281 cagtcagtaa agaagtgatt ttatgtctga catgatttga aatcgagtgc cttggacgga
     5341 taaaataact ttgagcgttc caatggcttc gacagttttt acaggtatag cgctgctttt
     5401 ttaggcgtat aaccattggc atatgattat attattcaaa gtggacaatg gtttccttct
     5461 tcttgttttt caccaccatt aatttcctat ttccatctac cacagcagaa ccacaatttc
     5521 tacagacaca tggagcaggg aagagaactg cagcaacgat caatgttttt ttcttctgta
     5581 aggtttcgta agagacctct gtaatcatca aatttttgtc tattgatctc agaatttttt
     5641 tgatagaacc attcataaag catatcgttc tctcagttgt ttattttgtg gtgatttaat
     5701 catacaagaa aacgatatgt ttttttataa aaacatgaat aggaaaaata gttgacaaga
     5761 aaatgacaac gccatatgat gttccatagt atttacaacc tgtcatagat gactgtaaaa
     5821 aataaaagta aaaagaaagg acgacaacag gtggattttt atcagtctct acaactagat
     5881 ccgtttattt taaaacaaaa aatacgagaa gcagcttcta aaaaagaaaa gtgcatgtat
     5941 atttgttcat tgtttttacg atcattattc atcgtattgt ttgccatttg ttttatcatt
     6001 ataattacaa cattatttga atcgaagcat aaaccatacg ctgtggtttt attctgtatg
     6061 ttgatgagta ttcgttttgt tgactttggt tataagattt cccattcgat cataggttta
     6121 gcaatcgtca tgttctcact attagtagca ccgtatgtcc agctgatcaa atggtcagtt
     6181 atgggcatgt tgattcattt tattttacta agcagcatat taatggcaac cgctagtgat
     6241 cccaaaatgg ggaatgcttc tttgtacggc ttttcatatc tctttattgt ttattcgtta
     6301 cctaaagatt cgttgaacaa gaatttcttt acacaaacaa gtagcttatt atttttgttt
     6361 ttttgttggt tttcagttct tctttacaga aaacatcgag aaaagaacaa agacaaaagc
     6421 ttgtttaaag agattttttt gaaaggcatg tattcgcagg aaaaaatttg gatgctcatc
     6481 tatgcatttg gcattagtct tttgatcgtt gcaggagagt atgtcccttt tcagcgattg
     6541 atgtgggcag ggtttgcatt ttcttctatt gtatctagct atggattgat gtcgattggt
     6601 ttcaaagaac gagcagtaga ccgaatcatt ggctcattga ttggatgtgt tttatttatt
     6661 ggtatcagcc aatttattcc ttttaactgg gtcggtatat taggtggtct agctttaggt
     6721 gtttgttcta cctatagata taaaacagtt tttaattctt ttggtgcttt ggcaatcact
     6781 gccagtttat tcggggttcc aggagcagta acgatccgca tttttgagaa tatccttggg
     6841 gtatgtttag gaatcatgta tattggggtg actgaaatac ttatccgaaa aataagagaa
     6901 aaacacggtt tgaatcatta gatgagatcg aataaaaaat aaggaaaaag tgaagataca
     6961 catggatgat aaattgctat cattccatgt gtatcttttt acggtaaatc agtgaaacgt
     7021 tcgactttca tttttaattt atacttttta cggagttcgt ttatttgagc catcatttcg
     7081 gatttgtgat gcttttcgat tgcttcttca tctttccaac ggtcgatcag taaaagagat
     7141 tctggatctt ctagtggaaa gtaatagtca tatttctcgt ttccttcttc tgcacgaatc
     7201 gctttgacaa tgtttctttc gaccatttct tctgcaaatt ttctggcttg tccatttgta
     7261 cttgtataca aaatattcac tgtgatactc actaaatccc ctcctgaaaa aattctatca
     7321 ctagtatatc acggtgccag aaattattat tcaagatatg atacacattt tttccgcaat
     7381 gctacggaaa tatatatttt gaatagaaaa cgcttacaag atatgcttgg tttataaaaa
     7441 taaaaggagt gattgattta tgaaaaaagc attgattatt tgtgcggcag gaatgtcatc
     7501 ctcgatgatg gcctcaaaga caacggattt ctttaagcaa aaaagagaag aggtactcgt
     7561 agatgcagtt tcagctacag aaggcgataa tatgattaag aatagtgatt ttgatttatt
     7621 tctgatcagc ccgcaaacga cgatgttttt ggataaattc aaaaaattag gagaatctgt
     7681 tggaaagcct gttgttagta tccctttcca agcgtatgtc ccaatcccta caggcatcca
     7741 gaagttagca gaggtgatcg aagagaacat atgatggaat aggagtggat gataatgaaa
     7801 cagaaggaaa aattgctgtt gagatatttg attgatcaca agaaggagta tgtcaccagt
     7861 caacgtttag cttctgaatt gttattatct gatcggacaa tccgtaatta cttgcagcgg
     7921 atcaaagagt tagtagaaaa aaacggaggt aagatcattg ctaaaccagg ttatggctac
     7981 caactgcata ttttacaaag actgacattt gacttgtttt tgagtcggca agaaatagta
     8041 cctggatatg ctagagaacc gcaaggattt tatgagtccg aagatcgaca aaagtatata
     8101 ttgaaccaat tgcttttgga agatgccatt ctttttatgg atgatttggc agaagagctt
     8161 tatatcagtc gttccagttt gacaaaggac atgcaagaaa tcaaggagcg attgattcca
     8221 tattcactaa aaatcgtttc taagcatggt caagggacat ggatcgaagg ggaagaaaga
     8281 aatcgacggc attttatcat ggatactttt tttggaaacc attatggaaa ttcattgaaa
     8341 gaatatttag ggcaaagccg ttttttccca gacatcagtt ttgaagagct agtcattatc
     8401 attctcgatg aaacaagaga agctaagtta aaagtttcgg attttatcat acagaatctg
     8461 actttacact tggctttggg gatcaaacgg ctccgagaag ggtttgaaat caaggaatta
     8521 ggcatcgaac aagaagtttc taagcgagta gagtatcaag tggcgcagcg gatcgttcaa
     8581 cgtattgagg taatcgcagg tgtatcgttt cccgaagaag aaattgctta tttgacatta
     8641 catctcatgg ctaagtcgaa tcacaaagaa aatcaaaatg atcaggaact atcaaaggaa
     8701 ttagcgaaag ttctgaaaag attgtctcag gaatttcatt tttcattagt agaggatctc
     8761 cagcttttca atggactact cgatcatttg tcaccgatga tgattcgatt gagccgaggt
     8821 attcgcattg agaacccatt gactgaggag ataaagaaag aaaatcccaa agtctttgac
     8881 gcagtaaaaa ggcatttcag caacatgccg gctttgaaaa actataccat caatgaagat
     8941 gaatgggcct atttggcttt gcatctcatg gcggcattgg aaaaagaaag agctgcgcat
     9001 aaactacatg cattgattat ctgtgcaacg ggttacggca gtgcccaact attaaagaat
     9061 cgcgtagtca gtgaattcgg taaaaatatt acagtggtaa gtgtaaaagg ctattatgag
     9121 atcaatgaaa caactttaaa aggaatcgac ctgattattt cttctattga tctttcgaca
     9181 atgtttttca aaattccagt tttacatgtc agtatttttt tgaatgaaga tgatgttcgt
     9241 aaaatacgaa aggtagttgg agaatctata ccctatcatt cctcagatag accgatacca
     9301 gcattttctc tacagcaaaa aaagcagatt tacaccgaac agttgtctga aaaatttttc
     9361 aagacttata cgtacgaacc gaaaaaggaa gaaattcttc atgatttgct gcaaaagctg
     9421 agcataaatg aagaagaagg atatataaga gaaatgcgaa agcagatcca gcaacgtgaa
     9481 aatatgggac aaatcatatt cagtgataca gtggcagtcc cacatcccgc tgttccagtt
     9541 ggaatcgaaa caaaggtagc cgttgcattg attccttctg ggatgcagtg ggatcaatat
     9601 caagaaattc attttgtttt tttagtttcg ccttcttatg tcgaaaatga aggaattacg
     9661 gtcgtgacta aagcaatcgt taggttagtt gaccagctaa aagtacagca ggaaatctta
     9721 gctgaaccta tttttgagaa tttcagcagt aaatttatca aactgatcga ataaggagga
     9781 aaacagatgg ctgaaaaaat aaacagtgaa acactacaag tgacttcttt tgatatcatt
     9841 ttccatagcg gaaatgcacg gacactgata catgaagcat ttcgctcgat gcgtaaagag
     9901 atgtttgaag aagtagaaga gaagttgcga caagcaaatg aagagattct tcatgcgcat
     9961 cagtcacaaa ctcaacttct gaaagaatat gccagcggga caaaaataga aatggaaatt
    10021 atcatggtcc atgcacaaga tcatctgatg acgacgatga cattattgga agttgcacag
    10081 gaaatgaatt atttgtatca aaaaaataaa tagaaaggaa ggatcattgt gcaggataca
    10141 gtaaaaagga aaatcgaatt agaaaaaaag caacttagtt tgaaaaacat gtatttcaat
    10201 cgttatctaa tcgtacgtta cctgactgcc tttttctttt tcatgaacat gcaatggatg
    10261 attctgcttc tatcagctaa aagtttagga agtagtttac cgatgattct gattttagct
    10321 attttgcctg cagtaggaga acaagtaaag ctttatcgaa aacaccaaac gaacgttcca
    10381 tggacaaagt ggtatttttt gttccagagt gtctttaatt tattagtgat tccagtgttg
    10441 tttacctcag gatttacatt cttgtatcct tttatggcaa ataacaatgg aggacagtta
    10501 tttgttttca ttcttgttgt aagcggtatt tttgccagtg catttataca gtatcgttta
    10561 aagaaaattt cactcaacca agaccaacag tatatccgta ttaaacaata tgaaaaagcg
    10621 ttatatttag gaaaggagaa taactaatgt ttgatttttt gcagaaatac ctgatgggac
    10681 ctatggggaa aattgcgcaa cttaaaatcg ttcgggctgt catggcagcg gggatggctt
    10741 ctattccttt cacgattgta ggctcgatgt ttttggtttt aaatattttg ccattaccgt
    10801 tcccattttt agaaggattt tttaatgcaa cttttttcaa agttagtgac ttgtacatga
    10861 tcgtcaatac aatgacgatg gggattttat ctatttactt tgccattgtg tttgcttatg
    10921 aattaacgtc aattgaacga gatgaacaaa aattaaatgt taatccgctc acgggggcat
    10981 tactatctgt ttttgcattc tttatgtgta tccctgaatt gatcatgact gatggtaaaa
    11041 tcagcttgat tgcttctatc aatgagacag aaactgtagt aaatggtatt aggatgggct
    11101 cttttgtcga acgtttagga acttcaggaa tctttacagc tattattatg tcgattattg
    11161 cggtcgaatt gtattgtatg tgtgtcaaga gaaattgggt cataaaaatg ccagatgttg
    11221 ttccgccagg agtttctcgt tctttcactg ctctgatccc aacatttgtc attgcgtttg
    11281 tggtgatgat catcaatgga ttattagttg cactaggaac ggatatcttc aaaatgattg
    11341 ctatcccatt tgggtttgtt acgcagttga cgaatacatg gcttggtgtc atggtgattt
    11401 atttcttgat ccatgcattg tggatcgtag ggatccatgg agcgaatatc atcacttctt
    11461 tcttgacccc gatcgtatta gctaatatgg cagctaacgc acaaggcgca aattatccgt
    11521 tggcaggaga attcaacaat tcttatgtaa ccgttggtgg atcaggtgcg actttaggat
    11581 tgatcatctt tattgcattc ctcgcgaaat ctgatcagct gaaagtgtta ggaaaagcct
    11641 cacttgtacc aggattattc aatatcaatg aaccaatcat tttcgggatg ccaatcgtct
    11701 acaatcccta tttagcggtt ccgtttttct tagctccgat ggcttctgct acactcgctt
    11761 attttgccat tcggctggaa atcgtcaaac cgatgcttgc acagatgccg tggccttcac
    11821 cagtcgggat cggagcattt gttggtagtg gtggtgattg gaaagctgct gtgttagcag
    11881 tcatttgtgc aatcatcgca ttcattatct ggttcccatt tatcaaattc tatgataata
    11941 aattattaaa tgaagaaaga gaaaaggcag cagaattagc tagtgaaaag ttaaaatgat
    12001 ttctaagaaa aaaggaactg tgacaaaaaa tcacagtccc tttttttctt agaaatggga
    12061 aaccactagt atataaatac cattattctt caatcaaccg cttcacgatt gcataatccc
    12121 cttcataagg ttcatctaca ccattgattt tttgataggg atccacgcct tttcccgcaa
    12181 taacgactga atcttgcgga gcgctttctg ctaaagcgcg tcgaatcgct tcaggacgat
    12241 ccatttcata atgaacgatc aaatctggat tagtaattgc ttcattgatt tcttccgcga
    12301 tttttttcgg atcttcaaat gctgggtcat ctgccgtcaa aaaggcgatg tcagctgttt
    12361 ctgatagcac ttttccaaaa tcatgtcgac gggagattgc tttgtttcct gggctgccta
    12421 atacaacaat tacacggcca tctggatgtt catttttggc aaattccagc aatgttttca
    12481 agcttagata attatgtgca tagtccacat agacatgcgc tccattggat tggatcaatt
    12541 gatccattcg gcctggtaca cgagcttctt ttaatccgtc ttgtgcatct tgactggagg
    12601 cacctgttag tgctgcggca atgatcgcac tggctgcatt ttcttggttg aatcctccag
    12661 ccagcaaaat atcataagca gtatcgcaga tagctaagcg atcttcatga gaagataaag
    12721 taaaaccgtg agtaccttta tctgaacgga tcacttgata attgctttct tccgaacgac
    12781 cataagtgat cgttggaatc tggaatagat cggctgtttc cctaagtaaa tcaaaatagt
    12841 cactttcatg atttaaaaca actgtttttg aattcaagat caattgtcgc ttgcaataga
    12901 aatagtcatc aaatgtcgga tgttcgatcg gactgatatg gtcaggcgag atattcaaga
    12961 agattccgac atctaaagtc aagccgtata cgcgttgtgt tttgtaagct tgtgaagaaa
    13021 cttccatcac tagatgagtc ataccatttt caactgcttc tgacatcata cgatataaat
    13081 ctaatgactc aggcgttgtc aaatgggatt tgaaatatgt tttgccatct aacgttgtat
    13141 tcatagtaga aagcaatgct gtttttttat ttgttgtgtg atccaagatt gcttttgtaa
    13201 agtacgcagc agttgtttta ccttttgttc cagtgaatcc gatcactttc aatttatttt
    13261 gcggatagtc gtaaaaagcc atagcaagaa gcgccattgc tttacggata tccgttacga
    13321 tgattcctac tgctgaattt tcgtatggtt gttctgatac ataatattga ataccctgtt
    13381 cgattgctga atccaaatat tcttctttga aattcattcc tttacagaaa aacaaagtag
    13441 aagcatcagc ttttcttgaa tcataactta actgaagaaa ttcagctttt tctggtacat
    13501 caagatgcca gcctcttgtt gaaacaaatt cttttaatag attttctttt aatagtaaat
    13561 gacggatgtc ttctaaagag aagctcattg attgatccct cgctttattc aatttcttgg
    13621 tttattatag cacactcttt ttaatatatc gtttgtttcc gctatgacaa aatccagctt
    13681 tattcaaatt ttcttgacac aggggacaag cggatgagta ttttcataaa caatgaactg
    13741 actgtaatag ataggagaac gaatagtgtc tccaacaatc caaaaacata ataattcaaa
    13801 aagacgataa gactattggt aagtaaaagt ccttgcggca ttggaagccg aaaaaatttg
    13861 tggaaaataa tcggcggtat cgttataccg ccatttgagg ccccgactgt gtacaatgtg
    13921 taaaccccaa gactgaacaa aagacttcct gaaatcagtg aaaagaaatg tgaggagatc
    13981 atttccactt ttggaacaat ctctaaaaca acaggaaaaa gcagacttcc cgctacagtc
    14041 ttaaaaaata ctgattttcc taataggaca gcggctagaa gcagcatcat aagattgatg
    14101 ctccaaacag taactgagcg ttggatagaa aaggccgttt caactaaatg tccgatggcg
    14161 cccactccgg cggaagctat atcgtgaggg agcaagaacc aattcaatcc aactgtgata
    14221 aagaaaagac ctaaaaatat aacgggtagt ttttttattt cttgtgtcat tgatatctcc
    14281 tataaatgtt agccataaaa ggctgtatat ttctttactt taccaaaaaa acgaaaccga
    14341 attcaaaaaa acaattgttc ttataaaagt tatataaaat cgattgccag tttctgtttt
    14401 accaattaaa atgatagtaa tgttttgtag aaagttggaa tctgaccgat ggataatcaa
    14461 agaaaaccag cattagatca attaacaccg caagaaaaaa tggcacgagg ctctgcttgg
    14521 atgaccgcca gtaatatcat ttcaagattg ctaggcgctg tctatatcat tccctggtat
    14581 gcttggatgg gcgaaaatgc taaggctgcc aatggattat tcaatatggg atacaatata
    14641 tatgcattgt ttctactgat ctctacagca ggaatacctg ctgcgattgc aaaacaaact
    14701 gcacgctata attcattgaa tgaatatggc acttcacgac gattatttat ccgtgcatta
    14761 cagatgatgg gcgggctagg cctgttgttt gctttgttca tgtacatcgc ctctccttgg
    14821 ctagctcatg cttctggtgg cggagaagaa ctgattccaa cgatgcgttc tttgagtatc
    14881 gctgtattgg tttttccttg tatgagtgtg atacgagggt attttcaagg caatcaagaa
    14941 atgatgcctt atgcgctgtc gcagattgta gaacaggtgg cacgtatttt ttatatgcta
    15001 ttggctacat tcatcatcat gaaagtcatg gatggtgact atgtgacggc ggtcacgcaa
    15061 tcaactttcg cggcatttat cggtatgcta gcaagtattc ttgtcctttt gtattttttg
    15121 aagaaacatc aagcgtatac atcggcgttt attcactaca gcgaaaacaa aataaatatt
    15181 gcaacgaagg aattgttgtt agataccgtt aaagaagcga ttccgtttat tattgtgggt
    15241 tcaggggtaa cgatcttcaa attagtcgat cagttcactt ttatgaagat catgtctgca
    15301 tccaccgagt attccaatgc acagttactg gatctttttt cgatttttag tgccaatcca
    15361 gataagttga cgatggtagt tattgcattg gcaacatcga tcgcgtctac tgggttaccg
    15421 ttgattacag aagctgttac gttgaaagat cgagtaggtc tggcaaaatt gacaagcgga
    15481 aatttacagt tgttttcatt ttttatgttt ccggcgacat ttggtgttat gttacttgcc
    15541 tatccgctaa atacattatt ttatacaccg gattctctag gaagtaatgt actgatccaa
    15601 gcaagttttg taggactctt cctaggacta tatatgctag tttccaacat gctgcaagga
    15661 atgtttgaaa acaaggcggc aattaattat ttggttattg ggtttctagt gaaactcctt
    15721 cttcaatacc cagccattcg tttatttgaa gtatacggcc ctttattggc gacaatgatc
    15781 ggatttgctg tgtcttgtac cttgatctta aagagaatcc atgaagtagc ccgctttaaa
    15841 ccaagttttg tctggcgcag gacattgctg atctttattc ttaccttgat tatgcttttg
    15901 gcttcgtgga tcacaaagat gatttttggt gcttttttaa acgaagatag caaattccag
    15961 tctctattgt tgattcttgt agtagcaggt ataggggctt tagtgtatgg ttatttagct
    16021 ttgaaaatcc gcttggcaga aagactacta ggaccaggaa tagcgcgttt acgacataaa
    16081 ttaaaaataa aataagtgaa tcagaagagc tagattgatg ctcttttgaa atgggggaaa
    16141 cgcatgcgtt tagataagtt tttagcagat atgggacttg gcagccgcaa agaagtcaaa
    16201 ggattgatca aaaaaggaac gatcgaagtc aatggacaag tggtgaaaag cgataaagcg
    16261 caagtaaatg aattcgaaga tgcggttact tatttaggag aaatcattac gtatcaaaaa
    16321 gatttttact acattttgca taaacctgca ggagtcattt ctgctactca ggataatcat
    16381 gatcaaaccg taatcgattt attggaagac caggattatc gggaagatct ttttccagtc
    16441 ggccgattag ataaagacac agagggcctc ttgattttaa cgaatgacgg ggtgtttgcc
    16501 catcagttac tatcacctaa aaaacatgtg gagaaagaat atctagctga ggttagcggt
    16561 ataatgacag aagaagatat ccgtttattt gcagaaggac tgattattga tggcgatgag
    16621 cagacattgc cggcaaaact ccaaattgat caagtagatc aagagacaga aacttcaatt
    16681 atccgtctga ttttgcatga aggaaagttt caccaagtta agcgaatggt caaagcagta
    16741 ggaaaagaag tcttatattt aaaaagaatc cgtatgggaa aattttggct gcctaaagat
    16801 ttgaataaag ggcaatatcg acccatgacc aaagaagagc tagaacaagt gagagaaaaa
    16861 taattgaatg gatagctaaa agaataagca gagtagttgt agcgccatct gaaaagcaga
    16921 cttgtccgtc tgactttcgg atggcttttt tgtgaaattt cggcttattt ccgtagaagc
    16981 tacttctgga acaccgtttg ttcggaataa gggactgtga caagactttt gttacagtcc
    17041 ccattgttcc ttgtgactca gagttagata gtaaggacac gatctcccta atactcaggc
    17101 caaaacgctg aattcacaac ctctgataaa cggtgttcaa tcagctgacc ctcggtatta
    17161 gctcaaaaaa cggcaaaata tgaggaacta ttttcccgtt tttttctcta atactcaggc
    17221 caaaacgctg atttcacaac ctctcctata ctttgtttca ttttccctat actttgttta
    17281 tttattctca ttattgaaag cgctataatt acgttgcgaa tgaatcgaaa ggggttttta
    17341 gatgaaaaaa cgaaaggggt tcaatcattt ttttatgaat ctatggcgtt ataaagcact
    17401 tgtcctgatg gctgtaccgg gtatgctttg gatgattttt ttcttttaca tacctgtaat
    17461 ggcgaatgtc gtagcattca aaaattttca tatttcagca gatgggttct tggctagctt
    17521 gagggaaagt ccttgggtgg gttgggaaaa tttcaaattt ttattttctt cggatcaagc
    17581 atttctcatc actaaaaata caattttata taatgtagca tttatccttt tgaatctgct
    17641 gatctcagtc gtatttgcaa tcattatgag cgagttgaga aataaacgac tcgttaaagt
    17701 ttatcaaacg atgtccttac taccttattt tctatcttgg gtcattatca gttactttgt
    17761 ttacgctttt ttaagtcctg acaaaggaat attcaatcag tggatcactg gacatggagg
    17821 agaagctgtc aattggtata atgaaccgaa atattggccg tttatcttgg tgttcattgg
    17881 tacatggaaa gggattggtt ataacagcat tatttacttt gcatctgtta tgggaatcga
    17941 tccgacttat tatgaagctg ctatggttga tggagctagc aaatggcagc agatcaaaca
    18001 tgtgacgata ccacaactta ttccactaat gacgatttta acgattttag ccgtaggaaa
    18061 cattttccga gctgattttg gcctgtttta taacattcct aggaattccg gtgcattata
    18121 tgatgtgact caagttttgg atacgtatat ctacaatggc ctgacatcca caggagattt
    18181 tggtatgacg gctgctgcag gactttacca atcagttgtc ggctttgtct tgttgatgat
    18241 cacaaatacg atcgcccgac gttttgatag cgattctgca ttattctaag gaggaaaaaa
    18301 atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt tcaataagac ggctaatttt
    18361 ttctctaata taatgattgc actctttgct atttcctgta ttttgccatt tatatttgtt
    18421 atcgccatct cttttaccga tgagtccata ttgtcgactc acgggtacag tttttggcca
    18481 gaaaagttca gtatatttgg ttatacgtac ttgttcgacc agatgcagga taaaattttc
    18541 caagcgcttt tcgtcactgt acttgttact gttttaggta cactgatcaa tagcacggct
    18601 acttctttat atgcttatgc gatttcacgg tcaaactttc cattccgtcg tttttttacc
    18661 gttttttgtt tgattacgat gttgttctca ccagggatgg tggccaatta tcttgttatg
    18721 acaaatcttc ttcagttaaa agatacgatt tgggcattga tcttgccaat ggctgtcagt
    18781 cccttcaata ttattgtgat gcgcacattc tttaaacgtt cggtctctga ttcgattatt
    18841 gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat ttgttcagat cgttttaccg
    18901 ttagctgttc caggtattgc gacaatcagt ttatttgccg cattgggtta ctggaatgac
    18961 tggttcaatg ctttactata catccaagat gataagctag ttccactaca gtatcttttg
    19021 atgaaaatcc aaagtaatat tcaatatctg acacaaaatt caggagcagg aagccaattg
    19081 acaggcggat tagcctcagt tccaggagaa tctgccagaa tggcgatcgt agtcatctcg
    19141 acgttgccta ttgcgatcag ctatccattt ttccagaagc attttgtcaa aggtctaaca
    19201 atcggcggag taaaagaata aagaaacaaa aatataaatc ataaaattag gaggaagaaa
    19261 aatgaagtta tggaagaaac tagcctttac aggtgtttca gcgttgttgt tgggaacatt
    19321 agcagcatgc ggaggttctg gttctaaaga ccaggcagaa gctagcaatt cggacacttt
    19381 gcagatgtat caaatcggag ataaaccaga taatttcgat caattgatgg aagtagcaaa
    19441 caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc aattacattg gctggggcga
    19501 ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa aattatgata tcgcctttgc
    19561 gaacaactat gtttccaatg cccaaaaagg agcctttgct gatttgactg aattagcgcc
    19621 taagtacgct gaaaaagctt ataatgattt agatgaagca tatattaaag gaaatctggt
    19681 caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc tttgctcaac aagtacttac
    19741 ttttaacaaa gctttactag ataaatacga tctttcaatt gatggaatcc aatcttacgc
    19801 tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa ccaaacacgg cagcttttgc
    19861 cattggacaa ggcttcaaag tccaagggaa ctttgattat cctttaggga atacgttacc
    19921 atttgctatt gatctggatg gagatgattc gaaaatcatc aaccaatacg ataatgaaac
    19981 gtatatcgga ctgttacgta caatgcatag ctggtacaaa caaggactca ttccaagtga
    20041 cgctgcaacg agcaataagg attatccact agaaggaaat acttggctaa tgcgtggaga
    20101 aacacaagga ccgtatgatt atggcgatac aattttgaca aatgcagcaa aacaagaact
    20161 tgtctcaaaa gcaattactg ttccactaaa atctacagct caagcacaaa tggcaaattt
    20221 tgtcgtatca aatacttcaa aaaataaaga aaaatcagta gaacttctag ggctgcttaa
    20281 cagtgatcca gaattattga atgggcttgt ttgggggatc gagggagaag catgggaaaa
    20341 agtcgatggt tcagatgata aaatcaaatt gctagatggt tatcagccaa atacacatat
    20401 gtctgcttgg aatacaggaa acaacaagat tctatataca caagagtcta ttacggatga
    20461 catgattgcc aaacgtgatc aatcgattgc tgatgcagaa acttcaccga ttttaggatt
    20521 cagcttcaac actgacagtg tcaaaacaga attaagtaat atttctaatg tcatgaatca
    20581 atatttagat ggattgaata cagggactgt tgatccagat gaaacattac cgaaattgaa
    20641 agacgctttg gacaaggctg gctatgataa ggtattgaaa gaaatgcaaa aacaatatga
    20701 tgagttccga caagaataat aatgaataaa gacaaagggg ttgtgacaaa agacgtgtca
    20761 caacctcttg ttccgaataa acggtgttca aaagccagtt tctcggtatt aagctaaata
    20821 tatgtaaaaa tgtggaaagc catattcaca aaatttttct tactactcga aactaagcgg
    20881 ctatttcaca aactcttggt aaacggtgtt caatcagctg accctcggta ttcgctcaca
    20941 aaacgacaaa atatgaggaa ctatttttcc gtttgtttct ctaataatca ggtcaaggtg
    21001 ctgattgccc aacctttttt tgtataatga gaaagtataa acttaatgtt tggaggagaa
    21061 gatatgttag ggaaggtttg gaagagttat ttatccggat ctgggtcatt ataaaactaa
    21121 ccttgtattt ctggatatat acatttgccg gcgggatcat ctttggatta ggtgcggcat
    21181 ggaaaacagt caatgaacta ttttatttgt acggattcga gtataaagaa atcacaataa
    21241 aaagaggctg gaatatatat aaaagaaatt ttttaagagg caatctgcta tttagtttat
    21301 ttttatctgg tactgcttta ttgagttaca atttatattt atctgttcaa atcaaagggc
    21361 tcattttttt agctattgat tttattttac tttttgcctt gctctgccta tttgctactt
    21421 atcaatacag tatgatttta gatagtgaat atacgatctc tgttcctaat ctgctaaagc
    21481 taagttttat ttcggttttt tccagctttt cgtcttttct aaaaataatt attggtagcg
    21541 gtataatttt aggcgtgact tggcaattca aaggattgat cctgtttggt gtcattggtc
    21601 ttttgacggt ttggaatggt acgatgacaa cacattggcg tgaagaatta gataaacagt
    21661 tggaaagtta tgaataagtt tcggattcta ttttcaaaag gaaccttact aaatcgtctg
    21721 ttgcggaaat acgcggttat catgatcggt gtaacgatga ctgctaccgt tatttttagt
    21781 atccatactt gggaacaaaa tcaaaaacag gcagaaaaca tgacaagtga tgccgttcaa
    21841 agcacaagca gaatgctgaa tgataaaaca accttgagta ggatcatcaa aaaccagctt
    21901 gtagggaatt ccgaaaagat cgaaaatgtg acgacctact taacaaaacc catagatcag
    21961 tatctgatgt atgtatacga acagcaaaac agtaccgatg aattagtttc tttcccaaat
    22021 caaatcaaag atctctacgc aaattatgaa gaactaagtg cgatatatat cgtgttgaat
    22081 caactaccgg agtattatga atcttctaga gaaaacaaag gtggggaaat aaaaaagggg
    22141 acaccaaaat tactagacgc cttttatatc aagttgccta tcacacaagg aggagctgag
    22201 tcaggaagta tttttattgg ttttcaaaag aaagaactag atatgatttt agaaaatcta
    22261 acgtcattca atggactctc tttatacatg atttctggaa cgacaaatcg actatacaca
    22321 ttccatgata ataaaatagc taaagaaacg tttgaaagac aagaaatgat catcacagag
    22381 agtctgaaga aaaattcaca attgccgatt gacgatctgg aaaaaaacaa ttttgttcaa
    22441 caccaagaac tgtctgatga ttatcgtata ttagctgtac tagataaaaa aagtgtaaaa
    22501 atcgaaacgg gaaaaaatct tgccccgctg atcattggta tcattatttt agacaatata
    22561 ctgcttatct ttctttataa aacattcaaa cgatattcac agcaagtatc tatcgtcatg
    22621 caggccatgg atcaaacagc agcagggaat ttagagacgc ggatcgacat aacaaacacg
    22681 gaatatgaac tgaaggaact ttcaatcggt atcaacgaaa tgttggatag catcaatcag
    22741 tttgtagaag acatctacaa attagaaatc aaacagcaag acgcacatat gcgggcgctt
    22801 caagcacaga tcaatccaca ctttttatat aatacattag aatatatccg aatgtatgca
    22861 atcagcgaag gaagtgaaga attagcagat gttgtgtatg cgttttccgc tttgcttcga
    22921 aacaatacga atcaagagaa aacgatcact ttaaaagaag aactggactt ttgcgaaaaa
    22981 tatgtctatt tgtatcaaat gcgttatcct aatcgagtag cctatcattt tatgatcgat
    23041 cctgatttgg aaaagatcga agtacctaaa tttgtgatcc agccattagt tgaaaattat
    23101 tttaaacacg gtattgattt tactcgattc gataatgcgc taagtgtcaa agtcttgcag
    23161 gaaggcaaac gggtaaggat catcatcaaa gataatggaa aagggatgac tgaaaaacgg
    23221 ctaaaacaga ttgaagaaaa actgtctcat cctaaagtgg aacttcacgg ttctatcggt
    23281 ctgcaaaacg tgaatgaacg tttacgtgcc agcttcggca gcagttatta tatgtcgctt
    23341 gagaataatg agacaggtgg actgaccgtt tctattacat tcaaggaggg atgagcgtgt
    23401 atcaagtgtt attggtagat gatgaataca tgattttgaa tgggttaaaa aagataattg
    23461 attggcaaag tttaggcttt caaatagtag caacagcaga gaatgccaaa gaaggactag
    23521 ctgtacttga acagcgacag attgatttag tagtaacgga tgtgacaatg ccagaaataa
    23581 atggactgga atttattgaa gcggctcaaa aagaacgtca taattttgaa tttatgattt
    23641 tatcaggata ccaagaattc gattatttga aaggaggtat gcaattaggc gccgtgaatt
    23701 atttgatgaa acccgtgaat aagttcgaat tggttgaaag cctcaagaaa atcaaaacac
    23761 ggttagatca gcaaaatgaa caaaaaaatc aacaagaaat ctatcaagaa ctgttattca
    23821 gtcaatggtt gaatgaagaa ttggatgaaa taagtgaaga agaaataata ggacaaataa
    23881 gtgagaagca gcataggata gtattgattc aactggcaag aactcatgat actcttatca
    23941 atagatggtt gcaagaacgg caacaaatct tttactatca acgaaattac ggagaccgtg
    24001 tattgatcac gttgttgttg gaggcagatg aagttgatgg cttctgttgt tttgttcaag
    24061 aaaactttcc tgatcaggaa tggctgatca gtatcgggga agagacagat tctttggaca
    24121 aaatccctga aacattccaa caagcgaaag acaatctaca gttgcatcaa ttttatggag
    24181 ataaagacga gcatgttttt tacgcagtac aagcagaaac aaaagatcaa accatcgatt
    24241 tttcaagttt cagacgcgtg ctgcaaaata aacaattgga agtcgctcaa aaaatgatca
    24301 ctgatttttt tgaacagttt cagttagcag ccatgatgcc ggaagatata cgatattcca
    24361 gttttttact gtttatggag attcaacggg aattgattga tctggaagat gaggaatatt
    24421 tacaaggaat cgaaaaaatc catcaggcaa aaacggtgca agaacttcag cagctacttc
    24481 tgtcattcat caaaaagcat cagagacaga aaaagtacag tgacaatgtg gaaaaagtaa
    24541 tcgtaatcct gcaccagcat tatcaagaac cacttacact caaagaagtg tcagaaaacc
    24601 tgcatttaaa tgtcatgtat ctaggacagc tgttcaaaaa agaaacgaaa aaaagttttt
    24661 cagcttattt gaaccattta cgtatggaaa aagcgaaaca actgcttttg cacagtaatc
    24721 aaaacatcaa tgagatagca agtgagattg gctacaacaa caccacgtat ttctctaaat
    24781 tattcaagaa aatcgtcggc agatccccta aagagtaccg agaaaacagt ggagaatcat
    24841 aaaaaaaaaa gccaatgtaa tgaaataact tataaaaaat tgtctagaaa tctaattttg
    24901 ggctgttctt attacttgag gcaaggcagc tttttcatga cctctcgata aacgg
//
LOCUS       DAZUKD010000033        24709 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000033 DAZUKD010000000
VERSION     DAZUKD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.033"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..530)
                     /locus_tag="ACG8N9_001130"
     CDS             complement(39..530)
                     /locus_tag="ACG8N9_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896744.1"
                     /translation="MTDKTGNLPKLAPKTSQTKSKKDEKIKNAVKKKSVKESSSLLLD
                     IPMSTFDEILDDNTDETSVKIASHVGMNSQNDSFYHLLNSLRELEDKKNQVIASKAPD
                     LTTDLDAEIIQVKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISEQKK
                     SDD"
     gene            613..1998
                     /locus_tag="ACG8N9_001131"
     CDS             613..1998
                     /locus_tag="ACG8N9_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4896745.1"
                     /translation="MKKSKKTFGTLLIAVMQIIGITASLITINPQVTHADGIATYDAQ
                     FNCSTEGSQGSSGSGKDSDGNETGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGIL
                     GNWKQESGNDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKN
                     FGGAWWTTEAQLDFMFKGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAV
                     VAVRGGNAKEIWEYMKSKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACA
                     TTDDANSGSGSMGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFN
                     GIYQGQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDG
                     WLWAVGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFS
                     GVGKAKFEIEK"
     gene            2021..2548
                     /locus_tag="ACG8N9_001132"
     CDS             2021..2548
                     /locus_tag="ACG8N9_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896746.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEVLTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            2535..3098
                     /locus_tag="ACG8N9_001133"
     CDS             2535..3098
                     /locus_tag="ACG8N9_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896747.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            3520..4257
                     /locus_tag="ACG8N9_001134"
     CDS             3520..4257
                     /locus_tag="ACG8N9_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896748.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(4274..4690)
                     /locus_tag="ACG8N9_001135"
     CDS             complement(4274..4690)
                     /locus_tag="ACG8N9_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896749.1"
                     /translation="MNKKRKLSVLITLISILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            4921..5085
                     /locus_tag="ACG8N9_001136"
     CDS             4921..5085
                     /locus_tag="ACG8N9_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896750.1"
                     /translation="MGKGRNYVTNIINYYPNRLPKGSFKKVGDKTYIITEEGIEALRS
                     QTENKNKKKE"
     gene            5082..6329
                     /locus_tag="ACG8N9_001137"
     CDS             5082..6329
                     /locus_tag="ACG8N9_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4896751.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            6599..8989
                     /locus_tag="ACG8N9_001138"
     CDS             6599..8989
                     /locus_tag="ACG8N9_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4896752.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKIKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            9038..10873
                     /locus_tag="ACG8N9_001139"
     CDS             9038..10873
                     /locus_tag="ACG8N9_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4896753.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            10948..11805
                     /locus_tag="ACG8N9_001140"
     CDS             10948..11805
                     /locus_tag="ACG8N9_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4896754.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            11795..12904
                     /locus_tag="ACG8N9_001141"
     CDS             11795..12904
                     /locus_tag="ACG8N9_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4896755.1"
                     /translation="MKNKILLFLTLICTLVLTIYPASQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            13243..14043
                     /locus_tag="ACG8N9_001142"
     CDS             13243..14043
                     /locus_tag="ACG8N9_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896756.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIEGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            14149..14937
                     /locus_tag="ACG8N9_001143"
     CDS             14149..14937
                     /locus_tag="ACG8N9_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896757.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            15050..16477
                     /locus_tag="ACG8N9_001144"
     CDS             15050..16477
                     /locus_tag="ACG8N9_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFS4896758.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            16708..17577
                     /locus_tag="ACG8N9_001145"
     CDS             16708..17577
                     /locus_tag="ACG8N9_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4896759.1"
                     /translation="MKKKEFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            17597..17944
                     /locus_tag="ACG8N9_001146"
     CDS             17597..17944
                     /locus_tag="ACG8N9_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896760.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            17957..19219
                     /locus_tag="ACG8N9_001147"
     CDS             17957..19219
                     /locus_tag="ACG8N9_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896761.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            19299..20675
                     /locus_tag="ACG8N9_001148"
     CDS             19299..20675
                     /locus_tag="ACG8N9_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4896762.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            20672..21301
                     /locus_tag="ACG8N9_001149"
     CDS             20672..21301
                     /locus_tag="ACG8N9_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896763.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            21371..21799
                     /locus_tag="ACG8N9_001150"
     CDS             21371..21799
                     /locus_tag="ACG8N9_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896764.1"
                     /translation="MGETIEYKDKNYIIIGIYNTRINRIGDPRITSDLVCQSVNYSPD
                     LTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNNEEKIFYQIYGIEKFEYE
                     HVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            22358..22957
                     /locus_tag="ACG8N9_001151"
     CDS             22358..22957
                     /locus_tag="ACG8N9_001151"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4896765.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            22973..23449
                     /locus_tag="ACG8N9_001152"
     CDS             22973..23449
                     /locus_tag="ACG8N9_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896766.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            23466..24368
                     /locus_tag="ACG8N9_001153"
     CDS             23466..24368
                     /locus_tag="ACG8N9_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896767.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            24441..24635
                     /locus_tag="ACG8N9_001154"
     CDS             24441..24635
                     /locus_tag="ACG8N9_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896768.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
BASE COUNT         9176 a         3591 c         4948 g         6994 t
ORIGIN      
        1 ccttaataaa caaaatttga aaaaagagta atgactattt agtcatcact ctttttttgt
       61 tcggaaattg aaatatgtgg agcttgtatt ggtttagtta ggattgcttg gctatatgga
      121 gctagttctt tatcaaaacc atcaaaatag gttttcagta gcgatttaac tgatcgctca
      181 tcttgtttga cttggataat ttcagcatcc aaatcggttg tgaggtcagg agcttttgat
      241 gcgattacct gatttttttt atcttccaac tctcttaggc tgtttaacaa gtggtaaaaa
      301 gaatcatttt gactgttcat tccaacgtga gatgcaattt taacagaagt ttcatccgta
      361 ttgtcatcaa gtatttcatc aaaagtactc attggaatat caagtagtaa cgaggatgat
      421 tcctttactg attttttctt tacagcattt ttaatttttt catctttttt gctctttgtt
      481 tgagatgttt ttggagctag tttaggtaga ttacctgttt tatcagtcat taattagctt
      541 ttcctttcta cttatttatg ttattataat atcataataa cataaaaatg tacacttttg
      601 ggggcgcagt tattgaaaaa atcgaagaaa acgttcggca cgttgcttat cgcagtgatg
      661 cagataatcg ggattacagc gagtttaatc acgataaatc cgcaagtgac acatgctgat
      721 ggaatagcta cttatgatgc acagttcaac tgttctactg aaggctcgca agggtctagt
      781 ggatcaggaa aagatagtga tggaaatgaa acagggagtg gtgctgtatc aggtgatgta
      841 gcaaaaaata ttgatgatat ttacaaattc atgaatgggg aatatgggct atcaggtgct
      901 actatcgcag gtattctagg taactggaaa caagaatcag gtaatgatcc tcgaacggta
      961 gaaggttatt acggtgatgg acagagagaa gttgacttta tcaaacaatt cactgatggt
     1021 ctgatgggta ttggctatgg tcaatggaca gcaggtcgcc atactgcatt agtaaactgg
     1081 gcgaagaaaa actttggtgg cgcttggtgg acgacagaag cgcaattgga ttttatgttc
     1141 aaaggcgatg gtggatttgt tgctatatta aaagattatg ctttaaacgc ttctgatgat
     1201 gttgtggcaa atgccgtagc gtttcataag aattgggaaa tttcagcaga ttccgaatca
     1261 gctgtagtgg ctgtacgtgg tggaaatgca aaagaaattt gggaatacat gaaaagcaaa
     1321 ggcatggacg gagctaaaga tgtaagcaaa atcaataaaa ttgccggagg aacaagtacc
     1381 ggtggtggat caaataattc atcaagctca gcatcaggaa cagtagaaga tgcctgtgca
     1441 actacagatg atgctaattc tggttctgga tcaatgggta aagaaggaaa aattaacggt
     1501 aaaaaaggtc aagtaatagc gaatatgact tatgaagaag ctttgaaaaa atatgggaaa
     1561 gcgatcacgc taccaaaatt caaagaacca gattggtcaa cttcaccttt taacggaata
     1621 tatcaaggtc agtgtacaga gttgacgtgg gcttatatga gccaattata cgatgggaat
     1681 caaccgacta ctggaaatgg tggctatgta tgggaaagct acaaatcggc aggagcgaaa
     1741 ataacgaaca aaccaatcgt aggttatggg tttagtgctt cggatggatg gttatgggct
     1801 gttggcgatg cagggcatac tggtgtagtt atagcggtgt ttgaagatgg gtcatttcta
     1861 tgtgctaact ttaatgttcc accaacccaa gctccaacaa ggggagtttc ggtaactttg
     1921 attgatggag tggacggatc agataatgtg catttcttta gcggtgtagg aaaagctaaa
     1981 tttgaaatcg aaaaataaat tggaatgaag gacgatatag atgaatagta aagtgaaaga
     2041 tacaattatc atttctttgc tttctggtat gttattagca ctgcttgttg gaatcctatt
     2101 cttcaataag cagaatcagg acatagaagc tgaaaaacac agtgtgcata ttgaagataa
     2161 gctgacattc tttaaagagg ttaaaccaaa tgaggtggat ggattacgga aagatttaga
     2221 aaatgatatt caagaaatgt tgaatggatc agttcaaaat aagtttgcag atcaatcagg
     2281 tgaagaattt ttgagaaaac tatttattgt cacaggtaaa ccaccaatta ctgataagag
     2341 tagtgataaa gaaaaaatcg agtactataa gccttttact tttaagctaa caaactttgg
     2401 aattaagaca tctagtagtg ggttgtatga agtattgacc gattttgaag ttcaatacaa
     2461 tggaaaaaaa gtgtatgata ccacattaac catcacacta aataatgata agaaagtgac
     2521 tgggggtgct ttttatgcaa aatcatgaaa tcactactgc taaaaaggcg attggaatac
     2581 tttccataat tgtgatcctt tgttgtgtgg tactatttca aaagaaaaat gcttatgata
     2641 ataatgtcaa agaattggaa gaccttaaaa gtaaacaaat cgaagtagtc aagaataacc
     2701 agaaagtgaa agctatgcag gaagcccaat acaaagaatt ggggaatgct gaaatcaagg
     2761 aacaaagtag gaaattcttt aatttatttt acaactggaa ttcatgggaa aaatatacga
     2821 agaatatgaa ggaaatacaa aaagaatttc ctcagatagt aaagtctagt cctgttgata
     2881 tttctggcac gatggtaggc actggtaata gtccaatatc tagctatact gctgattact
     2941 atacaacaaa tcaaaaaggt aaaatggttg aattgattac acagacaaga aatactccaa
     3001 caacagcaac aacaacaatt tggcgtggag taacaagcat aggagaaaat caattgtttt
     3061 acttggagga tttagatagc tatgtaagag cagagtaaaa taaaaaaaga gctaacggta
     3121 ccaacgttag ctctttgtgc tgacgatgat aacaaaacct accgaaaaag gattgtttca
     3181 tcacaaacac aaacagaaaa gaattgataa aaaacccgaa cctcagaatt tttattaatc
     3241 aattcattta gattataata aattgagctg aacattacaa tacgtatatg ttgtaaaacg
     3301 taaaaaaagt caaaagtaaa tactttctta aaaagtcaaa taaaactatt aaagacaata
     3361 tatatggttt atttgacata agaaaatggt ctagatgata attgtaatat agaattgtag
     3421 acttaaataa agcataaaat accttctatg ataaggtata gctcttaatt ttaagcagtt
     3481 ttagatataa gattgaaagt aaagtggggg agaataataa tggcagatcg taaattagaa
     3541 aagttattag aagaaacgtg gaatccaaaa gaatttagtg aattttttat ggagaatttt
     3601 gaaactgact tagcagtgat tgttaaagat gctcttagag agcaagggta tcctgaaact
     3661 gcaaactata taaacattaa ttttactttg tatactgaaa ataaaggaac ttgggatttt
     3721 tgggctactt tagcaaataa ggagttaagt gataaatcag acacaggaat tcgtaacttt
     3781 tttgaatcaa acagagatga ttatatgtat gctaacaatc aaaataaatt gaactttaga
     3841 gtagagtttg atgaaacacc tgaagaattt atagaaagac aaccgcctaa agaaaatgta
     3901 gcaaaggtat tggaagatcg ttggaattca gatgaaatag tttcaacaat tagcgaacta
     3961 ggcggacaat atgaaccgct agtagaagcg gttagagaag agctaagact gaataagttt
     4021 cctgatgttc aaaatattga tgtttctcaa attgaaataa acgtcaagat cacgaataaa
     4081 cttgattatg gtagttgggc agatatagca ttagaaaaat acatctactc aacgttaaaa
     4141 gagtttatcg aaaatagaat ggatatcatg tatctacagc atccacagta tttaaacttt
     4201 ggtgttgaaa tagctacacc tcttgaagaa tggaaaatgg agcaagggct tgactagcct
     4261 ttgctccatt tttttatttt acaaatgaat acagttcgtc gtttttacca agaatgattg
     4321 tttgcccaac tttgagattg tcaggtgatc cttcagcggt ttgtaaggag aacgttccat
     4381 tgaacttatc ggataccgtt ataggtagct ttaattctga tccttcctca tctttgagaa
     4441 ttgctgtttt tcgttcatca aaataaacag tctttattaa ataatttttc gtagtgatct
     4501 cagcagattt gtcttttttt gtagtagtac cagtttcttt agaattagag gtattttcta
     4561 cgactattcc atctttggca tttgagcttg tgtttatttg attgaatatt ttcagcgaca
     4621 agataccgct aagaatagtc aatatagaaa taagtgtaat taatactgat aatttccttt
     4681 ttttgttcat ttttcttaac ttcctttttt gttttgatta gttagatatt ccttatctaa
     4741 caagcatttt taggctatta aatgaagata aaccccaaaa aaatattgca attatgacag
     4801 tgcaatggta ctattattat acaataaatt ttgcgaaagg ggtaggtcta aatcgcaaac
     4861 aaaaagaaat tgaacctaga caaatacaac ttgtataccg caagggaagc aagtttgatt
     4921 ttgggtaaag gtagaaatta tgttacaaac ataattaact attatccaaa tcgtttgcct
     4981 aaaggttcgt tcaagaaagt gggtgacaaa acctatatta taacggaaga aggaatagaa
     5041 gcattgcgat cacaaacaga aaataaaaac aaaaagaagg aatgaatatg aagaaaaatt
     5101 cgcttggtat ggttttattt gcatcagctg tacttgcggg gttatcagta ccagtaacct
     5161 cacatgctga tgaatggact gcaaacacac cggatcaaat ccaaattaca acaggacaat
     5221 caagttatgc gttcaaaaaa ggagatacta tttggtcaac tgtagagaag cttaaaagct
     5281 taggttacaa gactactgtt gctgagtttg ccgaattgaa caatgttgat ttggcaaaag
     5341 gagaacaacg gactatccca attggacgaa ctttcaacat cagtaaagat acattaacag
     5401 ttaaggaagc agatggcgaa gtagttagtc aaactgttca aaaggataaa cctgatcctg
     5461 ttgaaaatac ggagcaagag aacaaaaaat ccgagactac aaaagaatcg gaaccaacag
     5521 caaacacttc tgataagaca gaaacaagtg cttcaagtgt tgatggagca agttcatcta
     5581 atacaaataa ttctacaagt gagaaagaag catcacaggg aacaagtgaa agtagcacta
     5641 cggaagctga tactggaaca tcaacaaata actcacaatc aacatcaaat gatgcaaatg
     5701 aatcaaccag tacatcttct agtgatcaat cacaaacagg ttcgagtgat gaaactgaca
     5761 gttctacttc accaagtaaa cccgaacaac caactaatgt tgtgctaggc gttccattta
     5821 ttagtcaagg taacacaatg ctttgcgaag gaacatcttt gctagaagcg ttacattata
     5881 aaggtgttgc aacaaatcaa gatttaatga cttttgttaa atcaatgcca ctatctcctg
     5941 ataataatcc aaataatggt ttttctggtg aatggagaca caatgtggat ggaacttatc
     6001 aaggtatgaa tccggctcct gtagtttcat ggggtgttgc taatggcgga aatgttgtag
     6061 atatttctgg ttcatcggta caaggattaa aagatgagat tgtaaatgga aatcctgtag
     6121 tagcatgggt aacttatgcc tttgaacctg ctcaacataa atcaatgttt tggggcgatg
     6181 ctatttggaa tcgacatgta gtagccgttg atggatttaa agacggtttt taccatgttg
     6241 tcgatcctgt gtttggggca agttggatta atgcaggatc atttgaaaca gcatataacg
     6301 tatctcaatt agcggtttcg gtacgttaat aaaagagatc gtttatatac taagtagtta
     6361 agtatagtct ttatttgccc ttacttaact acttagaaaa cttgttgctc atggtgggta
     6421 gaactaaaag gggtgatgct atgaataata gagcgttgaa acatcttgct ttcaacgaaa
     6481 aaagtgatta ataggtgtgg tgattttaac ttaggaatgg gcgcgaaggt cattaccaca
     6541 ccgacatatc gtggctcatt ttcttcgcct aactggtata aagaaggagt aacgagagat
     6601 gaagcatgaa aaaacgataa ttttaaaatc aaaaataatt tattgttttg cgactttagt
     6661 aatggtgtta tctacgctta taatgccttt ttcagcaata agagctgaag cagatgtcac
     6721 aaaaccgaca agtgcaaaat ttacagaaac tggcgtaaaa acagacggtt atattagggt
     6781 taatatgccg aatcatccaa atgaatggat gatttcaagt caatttaaag attctcatgg
     6841 aaatattgga tattgtatgg attcagaatt gccatcccca acaggaagtg gagccggaag
     6901 cttgaagtac aagggagctg gcagtgatga gttctatcgt atgtttaaag gtggttttcc
     6961 atcaaaaaca gcaaaggaac tgggtgcagg aaatgataca gaagcatggt atgcaacaca
     7021 attagtttca tgggttttag ccggaaactt taaagtttca cagattgtat ggtcacatcc
     7081 aaaccatacc gccgcagaaa cagctcgtgt aaaaaaagca tttgaaaaaa tttatgacta
     7141 tgctaagaat ggaaaagata cgcccaacac tgagttttct atcacagctt ctaagacagc
     7201 tgacgaagga aaatatcaca ctttcacata taaaacagca tcaaataaga ctggtaatgc
     7261 gaagctcact tttacatcag caaaacctgc tggtatgaag atttatgatg cagatggtaa
     7321 agaaattact aacaacactg taaaattaaa ttcaagtttt actattaaag tacctgtgac
     7381 tacaccaagt ggtactttgt catttaaagg tactgctaat gttagcacga ccaatccttt
     7441 tacttttgat ggacgaggtg tgtatcaaga tgccgtagtt atgatcacta cttcagaaac
     7501 aaaggactct aaatctttga gtgctaaatg gacaagagct gttggtcagg taacagttaa
     7561 gaaagttgat caagatggaa atgctttaaa aggagcggag ttcactctaa ctgacaataa
     7621 caatgctaaa tctgttaaga caaccggaga tgatgggtta gctacgtttg atttgcaaca
     7681 aggcttaaca tacagcttag aagaaacaaa agttcctgaa ggttatcaag gttcatttaa
     7741 aaaggatggt attactgtta ataccgatgg acaagaattt agttacactg ctacgaacac
     7801 aattaaaaaa ggtaaaataa aagttaaaaa ggttgaccaa gatggaaaac cattagctaa
     7861 tgctgaattt accttgacgg atgattcatc ccaaacacaa gcagaaaaaa cagatgaaaa
     7921 cggacttgca agcttcgata ttgtagcaaa caagacctac agccttgaag agaccggagt
     7981 accaactggt tatacaggcg cattcaagca agaaggaatt accgttaaag atgatggaca
     8041 aacttttgaa tatacagcta caaacacttt gaaaaaaggt gtagtaaaag ttcataaagt
     8101 cgatcaagat ggaaaacctt tggcaaatgc agagttcact ttaaccgatg aaaaaggtgc
     8161 aacacaagta gaaaaaacag atgaaaatgg acttgcaagc ttcaatattg tagcaaacaa
     8221 gacgtatagt ctagaagaaa ccggagtacc agtaggttac acaggtgcat ttaagcaaga
     8281 gggtatcagt gttaaagacg atgggcaaac ttttgaatat acagctacaa ataatttgaa
     8341 aaaaggtgta gtaaaagttc ataaagtcga tcaagatgga aaacctttag cagatgcaga
     8401 attcacctta acggatgaaa aaggtgtaaa agaagttaaa aagacagatg caactggtct
     8461 ggctacgttc aacattattg cgaacaaaac ttacaactta gaagaaacaa aagtaccaac
     8521 aggttacaca ggcgcattca aacaagaagg aattaccgtc aaagatgatg gacaagtctt
     8581 tgaatataca gctaaaaaca ctttgaaaaa aggtgtgatc aaagttcata aagtcgatga
     8641 tcaagggaaa gcattagcaa acgcagaatt cactttgacg gacgacaaag gtgtaaaagt
     8701 tgtacaaaaa acggacagaa atggcttggc aacatttaat attgttgcca acaaaacgta
     8761 caaaatcgaa gaaacacaaa accctaatgg ttatacaggg aaatggtcta aagaaggcat
     8821 caccgttgct gacgatggac aaacctttga atatacagta attaatcacc ttgtacctga
     8881 aacagtaaga acaggcggaa tcggtacatc agccttcctt cttgtatttc taattgcagg
     8941 attgagtatg gtgctactgt attatcgaga acagcacaaa gcaaaataaa aaagtgttaa
     9001 tggaaaagaa gtaaatatga aaaaagaagg ctgacgtatg aaatcaaaaa aaattttttc
     9061 aaatttaatg ctatttggta ctttgctagg agtgggattg ggcgcaagct caatctcttc
     9121 tagccttgga ctaggggggc aagtagcata tgcacaacaa aactataatt atcaaggaac
     9181 tggaacgatt catatccata aagtaacaaa tactaatggg gatgggaacc ctgtctcaga
     9241 tattgataac aaagggaaag aaatagcttt accaaatggt gtaaaacctc ttgaaggcgt
     9301 tgagttctct tatgtgaaac ttactaaaga tgaagctaaa tccttgggat ggacggatag
     9361 tgatactaca aaaggtcaac atattgatga tgctaaagca cgattattac taaacaaaaa
     9421 atacagtgaa gtaagtagaa tgaagactga ttcacaaggg aatatagaca tacctataca
     9481 agtaaataca gacgatttaa cagataactt gtatttgatt gttgaaacag gaacagtcaa
     9541 aggtgtagtt gaagaagcgt atcctatgat tgttaacgta cctgaactaa acggatcaga
     9601 tgatcaatac tttaattatg atgtacatgt ctatcctaaa aactatttcc aattaaactc
     9661 agcagagctt gataaaaaag acgaagatgg aaaggctctt gccggaactg tttttgaatt
     9721 gcgaaaagat gatggatctg atcaggttgt tggaacttat aaaacagaca gcacaggtaa
     9781 attaactgtg gatggtttgg aattaggtga ctattatttc ctagaaaccg atttgggaac
     9841 agatgctaat agaacaacct atttactaga tggtaaaaaa ttcaaattta gcgttaaacg
     9901 tggggagact gctaaagtag ttcttgatgt taccaactac aaacgacctg taattactaa
     9961 ggtaattgat aaagaaaccg ttgatactgg cgaagatgtt caatatacat tgaaaccatc
    10021 aattcctggt aatattggta attacgaaaa atatgttgtc aaagatgttc taggtgcaga
    10081 gttaacttat gattcatcag aagtgatcga aggaatgact ttagatactg attacagtat
    10141 cgaacgtgac ggacaaacga caaactatgt ctttactgaa tcaggtatgg ataagcttca
    10201 gaaacaattt gatgcagggg atcgcttctt tacaattttg atgaacgctc atactaatag
    10261 taaagctaaa atggatacac cacttaagaa tgatgtttca acgatctaca acaatggata
    10321 tgaacccgaa gacgaaacac cagttgatca cgcttataca atcattggtg atcaccaatt
    10381 cattaaaaca gatggagata caagcaaagc attgagtgga gcaaaatttg tagtgaagaa
    10441 caaaattgat ccgaataaag ttacttacct aaaacaagat ccaacaacga aagctgttac
    10501 ttggattgca gataaatcta aagctactga attcgtttcc ggatcagacg gtaagttcca
    10561 aattgaaggt ttgcaatata cctatgatgt agattttgat aaacaaggta atccaacaat
    10621 cacagataaa aaaatcaatg attatgctct tgaagaagtt gttgctcctt caggatatac
    10681 accaactaga aacgatgttc catttgtggt atcgaatgat gatgcttcta gcgaagtaac
    10741 agacatcaag aaccttaaaa cacctgaaac agttcgtaca ggtggaattg gaacagtagt
    10801 atttctagtg gttgcaggtg tggcaggaac agggctatat gttgtctaca aacgagaaca
    10861 acacaaagct taatgaaata aaattcccta gagtgttttt ggctctaggg tttcttttta
    10921 aaaagtgtgt gaaaagtagg tatgagcatg aaaaagaaga atatcaagtt gatcatctta
    10981 aaaattggga tgttgctatg tttaggagta ggtctatatc ctgtaattac tcaggcttat
    11041 acctactacc aaactcaaca aacactaaac tcctataagg aaaaatatga tgaagcttct
    11101 gaaaaggaga atgaagaagt cttcaagaga tattcagaag ttaataggaa acgaaaagac
    11161 acagaacaaa cctcagtcac gattcctgaa gataataaca atattccgtc aaatggaaat
    11221 cttcagaagg ataaattagg tgaattagtg ggtgaaattc ttataccaaa gattaatgtg
    11281 gatttgccaa tttatgatgg tgtaaacgat ttccaactat cagcaggagc aggaatatta
    11341 accggaactg attatccaac tggtgggatt ggaaaccatt ctgttatatc agctcattca
    11401 ggcattccta ataaaaaata ttttacagat ttagagttgc tcaaacttgg tgataaattt
    11461 tttattgata tttcagggaa aaaacttgct tatcaagtag atcggataaa aacaattcta
    11521 ccaaccgaat ttgaggattt gcttcctgtg gcaggaaaag attatgtaac acttatgaca
    11581 tgtacacctg ttggagataa ttctcatagg ttactcgtta gaggggttag cataccttac
    11641 caaaaaagta ttgttgagaa agaaaagaag aaagaagatt taaaggtaaa ggcgcagatg
    11701 aactggtatc ctatcgtagt tgtaggggtt ttattaataa tagctgtgat agtcattatt
    11761 aaaaaaatag tagcaaagag acgaggaatg aaaaatgaag aataaaatac tacttttttt
    11821 gactcttatt tgcactttgg ttttaacaat ttatcctgca agtcagctct atcatcaatt
    11881 caaaacaaaa gagaatgtgg cggaaatcca agagaaaatt gagaaaaaac aaactccttc
    11941 aaaagaggaa gtggcaaaaa aagtcgaaca aataaaatcg aacagcggta accaaggatt
    12001 agacaatact cagaaaacag ataaaggagt agacaatact caggaaacag acggtggatc
    12061 atttgatatt cgtgaagctg aaggaaaggt caatccatac acaggagatg ttttttcaaa
    12121 aaatcaaacg aaagattttg aaccatctac ctattcagga gcggttggtt atgtctatat
    12181 tccaaaaata aatgtgctag agcttttgtt tctaggcgct tcacaggagc atttatatag
    12241 aggtgtggca caaatgagtg ctactagtct acctttatca ggtaaaggtc atcaaacagt
    12301 aatagcaggt catcgtggcg gttatacgtc accacaattt ctttatgttg atcaattagt
    12361 agccggagac cacatttata tagattatct tggtgaaaaa gcagtttata cggttttccg
    12421 aacagacgtt attagaaatt acggtcagaa ttcaacactg actgatattc ctgatttaga
    12481 tatgttaaca ctttatactt gtcatccata tcctcagaac tacgatcgtt tattagttag
    12541 aagtctaaga gatgaaaaag caaaatacat cacgccacaa gtaacagttg gcaataccgc
    12601 atacgctgat gccattgata gtgaatccgt ttcagataag ccagaaactg ttacatcttc
    12661 taatgattta tttgaggatg ttgatcgtgc caacaaggaa ttagatggaa ataatgatct
    12721 taatattccg gaacaaggta gcgtagagat gcaagacaga acgtttgctg aaaaggcaag
    12781 ttcaatattt acaggaatta ccaataaaca tcagaaaatg cttttagtca tttggactgt
    12841 attaggagca ttagcaagtc taaaggttct tattctttta atcaaagagt ttagaaaagc
    12901 ataatgaaga ttataaaaat tgatagagta cagtaaaaag ggattgatac tattattgat
    12961 ctctttttta ttttgaatag agctagattc acttttaaat aaaatcaaaa ctgatcttaa
    13021 ttccaaagaa aaagtcaaga aaaaaataca acaaattttt ttatcagaat atttatcaga
    13081 atattattag aaaagataaa ctcaaaagct taatttaata gggttattgg ttgtatttag
    13141 aaataatgta tggaaaagct ataaagcaat agataagatg tagaaaatct tatttgtgaa
    13201 tgaaaaacaa gtcatcgtaa aatattaata ggaggtgtag taatggtgga aatatcaaaa
    13261 acagctttag actcattagc taaactattt agtagcaggt ttgatggtgc agaatttgtt
    13321 gaatatctaa gccaaaacga aagtggaaaa actctgatcg aaacactgaa aaaagactat
    13381 ttgaatgaaa atgactttcc gacatggacg aatattgaag atgctacatg ggatgtagtg
    13441 tgtacgatat attctccttg ggtaacgatt gctgattttg ttttagataa aattgaaggg
    13501 gatacatacc ctgatgccaa atctcttgct acaagtgact ttaaaacgtt ctatggttat
    13561 ctaatgggtc taataggcaa tggaatagat ttgaaagtta cgccaaaaca aacacctgaa
    13621 gaaattgaaa aagaaaaagg agaggttcaa atggcttatg cagatgacag agaaaaagta
    13681 aaattattgg aaggtaaatt atctgaagaa gaacttggaa aaatggtgca agaaatcaat
    13741 gaagacagca actattttcc tgaattgaag gtatatcaaa acgacgaaga tttctttaat
    13801 gaaatgttta gtaatagtcc atgggaagca gtgagtaaaa caaatttagg agaatggaac
    13861 tttcaagatg atctagttgc atttagcaat gatgggtatt tacgatcata ttcagaaagt
    13921 gagtatagag ctgaattaaa aacgtatgaa acggatattc tgatgagcta tttaaatcta
    13981 ttagagagtg gtcagggaga gttaagagat aaagttgaag aactattaaa agaagagcta
    14041 tagggattat ttcaacataa acacaagcag aaaactacca aacaaaacgt accatgcaaa
    14101 aagccggttt aaccggcttt ttgctctatt atgagaggag atcaaaccat gagtgcaaca
    14161 accgatatgg ctttagaagc agtaataaaa gataatacag gaaatgtcga aaatgtagaa
    14221 gactttagcg atattgcaat caatctttca gcatggattt cagctgtagg agaagaaaat
    14281 atcgaatttg agatcaagaa tatggatgac ttaaaagcaa aatttccttt tatcgcatcg
    14341 aaactaagtc aatttgccga ggagaaatcg tggaatgaat tagttagttt ttccgaaaaa
    14401 atttatgata ttacgcaaga agatgagtgt gtttcatggg aaatgaataa tcaatacaag
    14461 aaaattgatg ctttcgtcaa atcacgtata gaaggaacag cttttgagtt tgaaggtata
    14521 gataccgcag ataattggtt aaataaagaa tcctttatta aaagtatagc ggatgatctt
    14581 gaaaaacaat ggtctaagga agactatgca gagtacctaa aaacagaaag agatacagag
    14641 gtgaaagaaa aactcactga ggtatgtttg gatgaacata ttgatcccaa tatcgtaaaa
    14701 caagaagact atgaagtggt tattgataaa gttcctactg attttgagga agttgtaaag
    14761 tatatagttg aagaagcatt atcgcaatac tcttcagatg aggaagtgga cgaagaaact
    14821 gttcgtacca gagtttggca actggttttt gatgacaaag attataaata tttgagtgat
    14881 caaaactcgg ttaattggag tgtacgatta ttaattgatc aaaatgatat tgaataaaat
    14941 agagctgaaa agctctattc agactgtaga caaactccta aattgggagt gttgtttgca
    15001 gtttttttgt tttatactca ttgtataaac tattctggga gtgattctaa tgatgagaaa
    15061 acaatcgatt gaaggtagaa atcaatttgc tatgttaaca attgatgatc ttgtacctaa
    15121 agatcactta gttcgaaaga ttgatgctgc gattcagttt gatttcattt atcccattgt
    15181 agaatctacg tattcgaccc atggtcgtcc cagtattgat ccagtggtct taattaaatt
    15241 agtatttatc caatatttat ttggtatccg ttcgatgaga cagactatta aagacgttga
    15301 aacaaatgtt gcttatcgtt ggtttttagg ttattcattc gaggatccta tcccgcattt
    15361 ctctactttt ggtaaaaact atgttcgaag attcagagaa acaactttat ttgaagacat
    15421 tttctctcat attctagaac aagctgtaaa agctggcttc gttacggaag acaaccttta
    15481 tattgattct actcatatta aggcaaatgc caataaacat aagttcacaa aagaaatgac
    15541 ctacggtcaa gctaaagctt atcaagatga attagaagat gaaataaata aagaacgtat
    15601 tgctgtagga aaacgtcctt ttacatggga cacagaaagt gagttgagtc ttcaaaaaat
    15661 aagtcattca gatccagaaa gtggttacta tgttaaaggt gaacgagaaa aacaatttgc
    15721 ttattccgcg catacttcct gtgaagataa tggttttatc ttatctacat taatcacacc
    15781 aggaaatgta catgacagcc aagtcgcaat cgatctggtc aaacagtcga aaagagcttt
    15841 tccaaatata aagcgtgttg tcgctgatgc aggatataag acgcctaaat ttgttcattt
    15901 tctgtcacac ttgaaactct acccgattct tccatatact gtacctcatg gagtaaaagg
    15961 aatgtttcgt aaaaaagact ttgtttatga tgcctatttt gactgctatt tatgtcccaa
    16021 taatcaaccg ttgttatttt caactatcac tcgtgatggt tatcgaacct ataaatcaaa
    16081 tccaaaattc tgtaggagtt gtccattact taaaaactgt actttgagta caaaacatca
    16141 aaaaatagta actcgacatg tttgggcgga tcttatggat gaacttgaac accaacgcca
    16201 tacggcgttg aatagagaaa tttataaaaa gagaaaacaa accattgaac gaatatttgc
    16261 agatgcaaaa gaaaagcatg gtatgcgttg gacgaaatat cgaggtctcg aaaaagtcgc
    16321 cacgcacacg atgcttacgt ttgctgccat gaatttaaaa aaactagcca catggctatg
    16381 gaaagcaaaa aagcctatgg ctttttattt aaaatttcta caaagactaa caaaaaggag
    16441 ttggcaatta agccactcct ttttgtctac agtctgaata gagctgaaaa gctctatttt
    16501 ttttgataaa aaaactagct atttttttaa aaataatttg gttcaaaatt tattttttta
    16561 acttgataat tttttgccat ccttttttaa attggctata tatcaaggtt tatagtgggg
    16621 ataaataaaa atacatttga caaaatttac aatatagttt attatatatt tatcttatta
    16681 tgataacaca ataaaaggaa tgatggtatg aaaaagaagg agtttagtcg atcgttgtta
    16741 ggaattttag ttttagttgc ggttggtctt agtggcaagg gttactttca gaataacagc
    16801 actcaggaac atgattttca gatcaaaagt agtgttcagg taggtaataa agcagacagt
    16861 gaatcaaaag aatttttagg gatacgttat gatccaaaca aatatccaaa taatatcgta
    16921 aacattaatg atgggaaata tacactgaca aaagaagacc atgaagcatt atcagaaaaa
    16981 ggcagtcaaa ccttttgggt aaattatgca gatttagatc aacttggaag agcaggagat
    17041 gttgaagcgt tgatcaccta tgatgctgta aattctcact catctaaaaa tgtggaacgt
    17101 ccatctttca aaagttctgt ccatattgca ggagaataca aggatggagt atttgatcaa
    17161 gagagagata cttgggtcgg caaagattca aacaatgaaa tcactcagct agaaggatat
    17221 aaaggttatc tctataacaa aagtcattca ttagcatggt cgctaggagg agatatggag
    17281 gtacacaatt taacacttgg aacgagagcg caaaatgttg ggaaaaatga tcagaatgga
    17341 gggatggctt actcagaaac gattgtacgt gatgcaatct ataaaaataa gaatgaaaag
    17401 gtattgtata aagttgatcc aatttataaa gggaatgaat tggttcctcg tggttctcat
    17461 gtcgaagctt tctcagttaa cgataatgga aaaacggtaa accttaatgt ttatgtattt
    17521 aacaatcaaa aaggaatcaa catcaactat caaaatggta cttgggtaag agattagaaa
    17581 ttgagaaggt gaagaattgg aaacaataag catcaatgga ttagataaat cagttgcaaa
    17641 tcgaataaaa aaaggaaatg gctactatct tctgaagaag ggtaaatatt cagttgctag
    17701 agagttagaa acaatgagta tcgtgtatgt agctaagaat cattttgacg tagaacaggc
    17761 tatttttgat ccagattctg aaatagggaa atgtgttgaa ttatcagaga aaactgttag
    17821 tacaaaaggg gcgatgatca ttgagagaga aatgttagac gaaatcagag taatcattcc
    17881 taaagatacg cagatgtgtg aggacagcat aagtgacgat gaaccaaaat cagaaagaaa
    17941 ataaggagat attcagatga gcgaaaaatc cattgaatta aaggaatata agcgaagcga
    18001 tggagaaaaa tattatcctg tagccagttt ttctaaatca gtatatctgg ttggacgtgc
    18061 attggtagtt ggtgcaattg gcatatatat tttgcattgg gtaacaaagg ttgtacaact
    18121 gcttgtagat accttagtaa gtattcttgt ctcgttaaat gatgttaacg gaacagatga
    18181 tttaggatta aaagaaatgt ttaaagagtt actcaatgcg ttttttccca attatttaag
    18241 ttattcatat aatgtacaaa gcttttttgc aaagctacac ttggtagaca tctctatttt
    18301 agtattagaa gcaggaacgg tattctttgt tggatatttg gttactttga tgttcaggca
    18361 tcactatgga gaacaaagac cattttttga tgatatggaa agtagaaggt tgaaaagaga
    18421 agctctagaa gctatggatg tgaagtatga tccaaacaag attataggag atgaaacgaa
    18481 caacaaaaaa agaaaacgaa acaaaacgca attagaatcc gatgtgctat caacgcttaa
    18541 aaatatcaaa atttctgttc atacaagacg tgatctaagt gtcaatgaag atattcgaga
    18601 atatatcgta aaaatcccgc aaccaaaagg gaagaaagtt agagaaacag ttttacgaca
    18661 aattcaagat ttagggtcga tcctgacaag agcaacaagc ggacgagcca aatttggtga
    18721 aaaagaagat gaagtttcag gcgattattt cattttccga gcatcagtaa atattgaaag
    18781 agaacgtaag gaaagagtaa gtaccaaaaa caaagaaacg gaaagagcaa catcttcaat
    18841 cgcagtttca aattcacaat tttcttttcc aatcaatctc tttgccgata attccaaaaa
    18901 tatccaaaac caaaccgaaa aagctagaaa atatgccgaa actcaaaagc aatctttaga
    18961 actttatcta tcttctgctg acgtacctgc aagtctaaca gatatgcacg ttgggaatac
    19021 atctattgaa tatttgttta ctttatctcg taccagaaaa attcaactaa gctcaatgaa
    19081 agaagaaatt gaaacttact tgcatatatc aggtgtaatc gtgcggatca acgcagggcg
    19141 tattcaaatt actttaccat taggagaaaa tgatcgtatt ccaattgatg taccatcaat
    19201 gatcaatagc gtgttttaaa agacatcaca aatagaaaca gaaaatacaa actaccagtt
    19261 cacaaacaga aaagtaccga gaaaagtgag gaatagaaat ggctatgaaa ttattagaga
    19321 aagtcaaaaa gagagccgaa aaagctgaat ctattttgct agataaagaa cctaatataa
    19381 agcttaatgg aaaattcagt gtcaatatgt tgaacacaaa ggctaaagac ccgacagatg
    19441 ctatttttgg aattaagcct gatggcacaa ttgctcattt ttgcatcagc gaaatgccac
    19501 acatgttggt tgcaggaacg acaggatcag ggaaatcagt attactgaat gaaattcttg
    19561 ttacagcaat gtgtcactca acgcctgatg aattaaaaat aggaattgta gacccaaaaa
    19621 gggttgaatt tggaagatac aaaaaattgc cgttcatgtt ggctgatcca attacagata
    19681 tggatgaagc ttatgatttt tttgaatatc ttgtgatctt gatgcatgaa cgttacaaac
    19741 tgatggaaaa agcagggtta caaaacatca cgctatataa cgaatatgcc gaaaaaaatg
    19801 gattagatag atttccctat gtgatcttat tagttgatga atattcacag ttggttggaa
    19861 cacataaaga agtagaaggt ttgattgttc aattaggaca aatggcaagg gcagcaggta
    19921 ttcatgttat cttagctaca caatctcctc gatcaacagt tgtcactggt attatcaaag
    19981 ccaattttcc gtcacgtgtt tgccttatgg tagcaagtga tttagaaagt aggatcgtat
    20041 tagatgaagg cggtggagaa agcctaagtc caaagggaga tatgattatt aagctcgtta
    20101 atggaagtat ggttagagcg cagggagctt acatcagtaa caaagaaatt gaaacaattt
    20161 tcaatcacct tagaaatact atgccagaac cagaatatgt tgattaccga gcaatcgtag
    20221 ctgaatctcg tggtgagggt ggagaagacg aggattttgg tggaattggt aaagctcctg
    20281 taagaaaatc tgctaaaaga aatttgaaca cacctttagc aaattcatca tctaaagagc
    20341 catcaattga aaagaagagt acgtttgctc taaaacttga aaaaaacaaa gaagaaaaag
    20401 aaaaacaagg agctaaaaca ctagaaaaac ttcaagccga aaaattagaa aaagttttaa
    20461 aagcattaga taaagctgat tctgaaccgc aaacctcaaa caacacgaat aatgaacagc
    20521 cgatttcatc taaaactaaa ccgaaaacta ctgatccgaa agtttcagta aacgtgacca
    20581 tcacaaccga taagtctgag aagaaacaag taaacggaac gacttcacct atcatgcaaa
    20641 aaatgttgaa agccaaaaaa caggtgcata aatgatcatt gtcagttctt ttttgaggtg
    20701 gcttacgcca tataaatata cagatgaagt agttgtgaaa cgaacgaaga aaagagataa
    20761 aaaagcggta ctaactccta aacagatgct tgatcatggc attttagggg tgaaggtttt
    20821 agaagtgaat aaaccgtttg aatcaaatag tgcaagagaa aagttgcttg gagctgaagc
    20881 cgatgtcgca tatttttacc atgacaaaag caagcatcaa atagctatta aattcaatcc
    20941 aaaatcggca tggcggtatc aagcaagaga tgacttgaaa cgtaggaaac ttagaccaat
    21001 gctctatcca gaaggaaaag aagcatttga tagaacccac atcgttccca ttggttttca
    21061 tggtacagaa tcagataacc gcctattagt aggttggaac agcaatcaaa atagagttca
    21121 gatcaataaa tttgagaaga aagtaaggaa attcaacgat cgaaaaacga tcctatggtt
    21181 tgcagatatt tgtaaacagc cggatggaac agcaaagtgg gaagcggtga tatttgacga
    21241 aaatggtaaa aaagtaatgt ctgaaacatg gcatgataaa gctaaatttg tttggcagta
    21301 gcagtagagg tgaaaaagat tgctagaact ttttgaagca aatagaacag gctttaatca
    21361 aaaattagaa gtgggagaaa ctatagaata taaagataaa aactatatta ttatcggaat
    21421 ctataacacg agaattaaca gaataggtga tccacgtata accagtgact tggtttgtca
    21481 atctgttaac tattcacctg atctgacaag caggtataag aaatacgtgt tattagaata
    21541 tagacacaag attactgatg aaattggtgt aaacaacaca cgaaacatat ttaagatcgg
    21601 aacagtgatt ccttatagta acaacgaaga aaaaatattt tatcagattt acggaataga
    21661 aaagtttgaa tatgaacatg tcgatctgct agtgacgtac caaatgcgtt taatagaacc
    21721 ttggtcacag gcagaaattg ataaagcagt aaaattgaac cgattatcaa aatttaacgt
    21781 attaggaaat gccttttaaa aatcgcaata cacaaacaga aaaataccaa aaaattagca
    21841 atgaacgtag gtaagaacat gacaataaat aataataacc ctaaaaaata taatgctctt
    21901 gtccaatgct atatcgaaaa caaaattaca atgcaagaag tcgctgatga attaggcaaa
    21961 tcaaagagcc tagtatcaca cgattttaag cacaatgttt ttccgaaccc gattgagctg
    22021 aggaaaaaat ttcaaaagag agtttatcaa gaaataggta agtggattga tgaagggaat
    22081 gcaattgacc ctttaattga aaaatatcct tttttaaaaa gcaaaaataa aattcaaagg
    22141 ttaattcgtc aaggcgatat tcaagctaaa gaacctgttt tgacaaccaa tgatttggct
    22201 aaaataaatc ttcataaaca aattattgaa gactttgaaa aaaatctttc aacaactgaa
    22261 attttaaaaa aatacaatat tccgagagga aagctaacat cgactctttt tagagaatat
    22321 ggagaatcgg cagttactaa agcaaaggaa gtagcgaatg agagaaaaaa acaaaaatag
    22381 tagtcgatac ctagtagcga ctgtagtttt tgtttgtgta ttgattttcc tgtttgattt
    22441 tttgcagatt ggaaaaagca taattggaaa tacattgagt gcaaaggaaa tggttgcttt
    22501 aggtggtttg tatgaaggac acttgttccc gaatatattc atttctatcg tttctcatat
    22561 taacttgtac catttaattt ctaatttatt gttcttagta gttcttgaag aagcactgaa
    22621 tgatcagtat gagtggttaa cattattgat cgttttaatc atatcaggat ttctaggaag
    22681 cttagttcct tttctgctac acagcaacat cgtaagtatg gggcttagtg gaggtatttt
    22741 tggatggatt gctttatttg cgattgcata catccgtttt gatagcaaga aacatttatt
    22801 actaagtgtt tttgtattat tgataatcgg cattttactg gtatctactt ttgaatcagg
    22861 cgagacaaac gtcttgtcac atgtaatcgg atttgtctgt gggatgatct tgggatttat
    22921 tctaaagaac gtttcattga tcaaacaaaa gaattaaaag ggagtgctaa aaatgccaaa
    22981 aaaaaaagaa aagaaaattc cagtgttttt gtctgtctca gaaatgttat cacttcaaat
    23041 actgttaaga aaaaagattg aaacaataga cgaagaagtt agaaataaaa gggctatgta
    23101 cgaacaacgt gaagcgctaa gagaacgaat ccactatttt gatgatatac acactaaaat
    23161 ctatgaagca cgtacaaatc acgaagacga aagcaaatta ggattagctc aacaacgaat
    23221 atttgaatgg ctgaatacga atactgagca agaaccgtta agagcaatta gtaatctttt
    23281 tgaatttgga acgaagcatt ggtttaacgc tgtagaaaag gatgctgact tacaagaagc
    23341 gttcgataca ctaagcgagg atagcaaatc catgcgagac ttattgatca cttatctaaa
    23401 tgagcatgaa tttgaacaga cgaaagttaa aaagaaaaat aaaaaataga aagttgtgat
    23461 gtatcatgct gtttacctta ttaggttatt tatgtttagt tcttgctttt tttggagcaa
    23521 ttgctatatt tgtcggtcag tttcaagcaa ataatccgat aatgcgtaac ataggcattg
    23581 gaattgttat catatccttg attgtatttg gtgccacaaa gatttttggg agtaatgatc
    23641 agcagtttgg tatttctgga gttgataata gttatgctcc agattcattg ggaagagtag
    23701 tgatagatgg aacgtttgag aatgtaacga gctacaaaaa tgtttcattg acagtgaaag
    23761 aagatgacaa gaaagcatca gatgacgttc tcgctacgat caagccgtct caatcacagt
    23821 ttgaagtgtt ttatacagtc aaagagaata accctgtacg caagctaaca ttagttttta
    23881 acgttgacgg agaagaatac tataaagatg taaaaatcaa taaagcatca acgaaaaaga
    23941 ttgtcgaatc agcgaaaaaa gaagtagcta cagaagaaag tacaactgaa tcaagttcta
    24001 ctgtcgaagt tgaactacca gataaaattg attttgatcc tgaaaaagtc aaatcgtttg
    24061 aatctgtttc tggttctatg gcagatcggt ttaacactga agttttcaaa gctatcaaaa
    24121 ctgttaaatt ggatactgaa aagcaaagga ttaatgttac cttattcaat gcagttgaat
    24181 caatggatca ggataaaaaa gttacggttg caagagaact agggaaaaga gtaattgggt
    24241 tgtatcgagg tacggcaaaa ccagattcag acggttcaga tttgccagtt cacgtagttt
    24301 atgaagacgg taaagttttt ggagactcta cagcaaatga agcactaacg attaaattag
    24361 ctaaatagcc ggaaaattat aataaacaag tggtgtcaca ccaccatcgc ataggtgtga
    24421 caccttggag atgattactg atggtagtaa ataaagaaaa agtaagaaag tatgttactt
    24481 taaaaatagc ttctgttgaa tatttagaaa agcgagctaa agaagaagaa aaaacaagag
    24541 gtaagaaggt aacagtttca gagttgttag aagaattaat tgagagtgac caaacaataa
    24601 gacgtgcatt taccaatagg tctgttaaaa gttagggtga tactatcttt aatttcaacc
    24661 aaatttctaa tatatgatga atagtttcct tgtacaaaat atctaatta
//
LOCUS       DAZUKD010000034        24576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000034 DAZUKD010000000
VERSION     DAZUKD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.034"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..552)
                     /locus_tag="ACG8N9_001155"
     CDS             complement(154..552)
                     /locus_tag="ACG8N9_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4896769.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="ACG8N9_001156"
     CDS             complement(549..1322)
                     /locus_tag="ACG8N9_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4896770.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="ACG8N9_001157"
     CDS             complement(1319..2002)
                     /locus_tag="ACG8N9_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4896771.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="ACG8N9_001158"
     CDS             complement(2169..3056)
                     /locus_tag="ACG8N9_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896772.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="ACG8N9_001159"
     CDS             complement(3053..3922)
                     /locus_tag="ACG8N9_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896773.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="ACG8N9_001160"
     CDS             complement(4059..5345)
                     /locus_tag="ACG8N9_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4896774.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="ACG8N9_001161"
     CDS             5618..6196
                     /locus_tag="ACG8N9_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4896775.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="ACG8N9_001162"
     CDS             6196..7125
                     /locus_tag="ACG8N9_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896776.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="ACG8N9_001163"
     CDS             7122..8858
                     /locus_tag="ACG8N9_001163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4896777.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ACG8N9_001164"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ACG8N9_001164"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4896778.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="ACG8N9_001165"
     CDS             complement(9754..10263)
                     /locus_tag="ACG8N9_001165"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4896779.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ACG8N9_001166"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ACG8N9_001166"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4896780.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="ACG8N9_001167"
     CDS             complement(11564..12655)
                     /locus_tag="ACG8N9_001167"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4896781.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ACG8N9_001168"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ACG8N9_001168"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4896782.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ACG8N9_001169"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ACG8N9_001169"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4896783.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ACG8N9_001170"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ACG8N9_001170"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4896784.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="ACG8N9_001171"
     CDS             complement(16618..17592)
                     /locus_tag="ACG8N9_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4896785.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="ACG8N9_001172"
     CDS             complement(17573..19456)
                     /locus_tag="ACG8N9_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4896786.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="ACG8N9_001173"
     CDS             complement(19474..21699)
                     /locus_tag="ACG8N9_001173"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4896787.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="ACG8N9_001174"
     CDS             21814..22848
                     /locus_tag="ACG8N9_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4896788.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="ACG8N9_001175"
     CDS             complement(22915..23928)
                     /locus_tag="ACG8N9_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4896789.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="ACG8N9_001176"
     CDS             complement(24006..24194)
                     /locus_tag="ACG8N9_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4896790.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7062 a         5340 c         4397 g         7777 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaaat
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaact
//
LOCUS       DAZUKD010000035        24309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000035 DAZUKD010000000
VERSION     DAZUKD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.035"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..800)
                     /locus_tag="ACG8N9_001177"
     CDS             complement(<1..800)
                     /locus_tag="ACG8N9_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4896791.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKV"
     gene            complement(1024..2964)
                     /gene="thrS"
                     /locus_tag="ACG8N9_001178"
     CDS             complement(1024..2964)
                     /gene="thrS"
                     /locus_tag="ACG8N9_001178"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4896792.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3301..3480)
                     /locus_tag="ACG8N9_001179"
     CDS             complement(3301..3480)
                     /locus_tag="ACG8N9_001179"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4896793.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3527..5350)
                     /locus_tag="ACG8N9_001180"
     CDS             complement(3527..5350)
                     /locus_tag="ACG8N9_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4896794.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5628..7907
                     /gene="helD"
                     /locus_tag="ACG8N9_001181"
     CDS             5628..7907
                     /gene="helD"
                     /locus_tag="ACG8N9_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4896795.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8000..8647)
                     /locus_tag="ACG8N9_001182"
     CDS             complement(8000..8647)
                     /locus_tag="ACG8N9_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896796.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8826..9545)
                     /locus_tag="ACG8N9_001183"
     CDS             complement(8826..9545)
                     /locus_tag="ACG8N9_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4896797.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9676..10827)
                     /locus_tag="ACG8N9_001184"
     CDS             complement(9676..10827)
                     /locus_tag="ACG8N9_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4896798.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11044..11784)
                     /locus_tag="ACG8N9_001185"
     CDS             complement(11044..11784)
                     /locus_tag="ACG8N9_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4896799.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11788..12111)
                     /gene="rsfS"
                     /locus_tag="ACG8N9_001186"
     CDS             complement(11788..12111)
                     /gene="rsfS"
                     /locus_tag="ACG8N9_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4896800.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12168..12761)
                     /gene="yqeK"
                     /locus_tag="ACG8N9_001187"
     CDS             complement(12168..12761)
                     /gene="yqeK"
                     /locus_tag="ACG8N9_001187"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4896801.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12745..13395)
                     /locus_tag="ACG8N9_001188"
     CDS             complement(12745..13395)
                     /locus_tag="ACG8N9_001188"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4896802.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13405..13716)
                     /gene="yhbY"
                     /locus_tag="ACG8N9_001189"
     CDS             complement(13405..13716)
                     /gene="yhbY"
                     /locus_tag="ACG8N9_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4896803.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13732..14841)
                     /gene="yqeH"
                     /locus_tag="ACG8N9_001190"
     CDS             complement(13732..14841)
                     /gene="yqeH"
                     /locus_tag="ACG8N9_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4896804.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14838..15365)
                     /locus_tag="ACG8N9_001191"
     CDS             complement(14838..15365)
                     /locus_tag="ACG8N9_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4896805.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15545..16330)
                     /locus_tag="ACG8N9_001192"
     CDS             complement(15545..16330)
                     /locus_tag="ACG8N9_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4896806.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16323..17192)
                     /gene="accD"
                     /locus_tag="ACG8N9_001193"
     CDS             complement(16323..17192)
                     /gene="accD"
                     /locus_tag="ACG8N9_001193"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4896807.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17193..18572)
                     /locus_tag="ACG8N9_001194"
     CDS             complement(17193..18572)
                     /locus_tag="ACG8N9_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4896808.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18580..18999)
                     /gene="fabZ"
                     /locus_tag="ACG8N9_001195"
     CDS             complement(18580..18999)
                     /gene="fabZ"
                     /locus_tag="ACG8N9_001195"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4896809.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19024..19500)
                     /gene="accB"
                     /locus_tag="ACG8N9_001196"
     CDS             complement(19024..19500)
                     /gene="accB"
                     /locus_tag="ACG8N9_001196"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4896810.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19503..20738)
                     /gene="fabF"
                     /locus_tag="ACG8N9_001197"
     CDS             complement(19503..20738)
                     /gene="fabF"
                     /locus_tag="ACG8N9_001197"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4896811.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20757..21494)
                     /gene="fabG"
                     /locus_tag="ACG8N9_001198"
     CDS             complement(20757..21494)
                     /gene="fabG"
                     /locus_tag="ACG8N9_001198"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4896812.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21506..22423)
                     /gene="fabD"
                     /locus_tag="ACG8N9_001199"
     CDS             complement(21506..22423)
                     /gene="fabD"
                     /locus_tag="ACG8N9_001199"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4896813.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22530..22754)
                     /locus_tag="ACG8N9_001200"
     CDS             complement(22530..22754)
                     /locus_tag="ACG8N9_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4896814.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22807..23769)
                     /locus_tag="ACG8N9_001201"
     CDS             complement(22807..23769)
                     /locus_tag="ACG8N9_001201"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4896815.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23779..24219)
                     /locus_tag="ACG8N9_001202"
     CDS             complement(23779..24219)
                     /locus_tag="ACG8N9_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4896816.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6650 a         5423 c         4238 g         7998 t
ORIGIN      
        1 actttttcgc taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta
       61 gcaaattggg ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta
      121 gatgtttgta cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac
      181 gcaatgattt cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt
      241 ctacctgagt ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg
      301 agcaagacgt ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc
      361 agaatatctt ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc
      421 gattccaaac actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct
      481 tttatcgcta cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt
      541 gcttctatat cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt
      601 ttgaagtccg tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca
      661 ctcaacccgg aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta
      721 ccgataatgt agttggccaa ctttttttct gctgaagaat aatcttccat catactttca
      781 atacgactaa ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata
      841 ccatatcaga cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc
      901 aacagaatat cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga
      961 atacctgtta tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac
     1021 ctattagttt tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt
     1081 caaatcttct acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc
     1141 caattccttg tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat
     1201 cttatagccc attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa
     1261 acgttctttg atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt
     1321 tgcttgtata ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc
     1381 gacaaaacgt tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt
     1441 ttccccgtct tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg
     1501 tatagttgat aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg
     1561 tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc
     1621 ggctttcagc attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt
     1681 attgtttgga tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata
     1741 tactgccacc atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac
     1801 aaatgtatga ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc
     1861 tgatttttca tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata
     1921 agaatggatc gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac
     1981 aagcatttcg ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca
     2041 gtgtccagat gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta
     2101 acctagacta acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc
     2161 ttttggaagc cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag
     2221 ttcttttcct agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc
     2281 tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt
     2341 tgagtttcca cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc
     2401 agtggatgga acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt
     2461 aatcacttct tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa
     2521 aatttctaat gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac
     2581 gattttcatc atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc
     2641 attatctgta tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt
     2701 tggatagagg cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc
     2761 aagtgcatct tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg
     2821 agtcaaatca acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt
     2881 tgaaatgctt tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc
     2941 atctggaaaa gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga
     3001 tcatcaagct gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa
     3061 agttcgtggt tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc
     3121 gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa
     3181 agtttcagcc tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat
     3241 caatcaatta tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac
     3301 ttattttttc cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac
     3361 atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat
     3421 catattttct aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat
     3481 aatgctctct cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg
     3541 tcttgggtac tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa
     3601 tagagaccgt atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg
     3661 tacttgcaat taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg
     3721 ctattctcat cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt
     3781 tcaagatgac cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa
     3841 taccgatatc tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa
     3901 caggtctttg atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac
     3961 gggcatgtac agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat
     4021 ccccggtgat catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg
     4081 ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg
     4141 ttacatagat tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc
     4201 caatctttac ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca
     4261 gttgactaac aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga
     4321 tatggctaga ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc
     4381 cattgactgg ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat
     4441 caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga
     4501 tcccgttttt acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca
     4561 gtggacaagg tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc
     4621 accaagaaat accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag
     4681 ctaaacggac aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt
     4741 gatactggct gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta
     4801 aagaccctga cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca
     4861 aagaagattc atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt
     4921 cgccaggctt gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca
     4981 attttccttc tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt
     5041 ctttacccgc tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca
     5101 atatcatcaa actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg
     5161 ctaatacatc tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca
     5221 agaaggcgag aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa
     5281 aaacaaattc acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga
     5341 aatgtgtcat tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc
     5401 agctgcctct ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt
     5461 aattgaaaac aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat
     5521 attcagcaag gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta
     5581 tgctatactt ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac
     5641 aactagaaca aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc
     5701 tggatacaaa acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta
     5761 atcacaagat tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc
     5821 aacatgagca ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc
     5881 aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc
     5941 gtgaaacaga aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa
     6001 tcgttattga ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg
     6061 cattttatga aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta
     6121 aaatccaaga aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact
     6181 ttttgctaga gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga
     6241 tacaaaaagc acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag
     6301 gaatcgccgg aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc
     6361 ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt
     6421 ctgattacat ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat
     6481 tcaaaaatta tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag
     6541 aaagtggttt tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct
     6601 tagtcgatca gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg
     6661 atatgaaaat aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag
     6721 aaacaaatga acaattacct ttacatcata gactttctct attacaaacg aagctcctga
     6781 aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg
     6841 aagaacagct ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg
     6901 agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc
     6961 atcgagggat catgaattat caatttgtca atataacgaa acaatatctc cactttctac
     7021 aaactgtgcc actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac
     7081 atttgacaac tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac
     7141 tattcttttt actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata
     7201 tctttattga tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt
     7261 tcccaaaagc aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa
     7321 cgatcgttgg atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa
     7381 cgacaagcta tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag
     7441 aacatcaagt agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa
     7501 cagatgatat caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc
     7561 ggtattggcg tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc
     7621 aactgccaga cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga
     7681 agcgttccat tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt
     7741 tcttgtggga tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca
     7801 ccatgtgtac acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt
     7861 tgttaaacaa tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac
     7921 cgctatcatt aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa
     7981 ttcaatcatt taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta
     8041 atttgacaag cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa
     8101 ataccttgct atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt
     8161 caatgaattg gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct
     8221 ctgatacacg aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct
     8281 tctcatttct ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta
     8341 gaaagaaagc ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac
     8401 gaaaaagcag cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca
     8461 tacactctaa ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt
     8521 caacgccgaa tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta
     8581 actcttctac tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg
     8641 cttccatatt cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat
     8701 gctctcatca taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac
     8761 aatctgttag tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg
     8821 gttgcttagt cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc
     8881 agtgcatcaa tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt
     8941 tgagggatca tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct
     9001 tcttgtatac tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc
     9061 attacatcgc gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct
     9121 tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca
     9181 cccatgttcc cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca
     9241 ttgttcgtca atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc
     9301 aattcagagt atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata
     9361 tgttttggta cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca
     9421 tgcggatcag gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa
     9481 atccggctat tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt
     9541 cccatatttt cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc
     9601 attatacttg tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca
     9661 tttttagtca attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt
     9721 gcctcccaat tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt
     9781 ttttccgatt cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc
     9841 atcggtaaat ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt
     9901 tttgatattc agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt
     9961 tgtttttacc tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat
    10021 gcgcgattct attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga
    10081 taacaaccga taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga
    10141 aagatcttct tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc
    10201 atggcgaatg gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc
    10261 tgcactttta cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt
    10321 tgcataactt aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata
    10381 cagactgcga aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc
    10441 ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta
    10501 atcaaacgaa gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa
    10561 gatttttact gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag
    10621 ctccactact aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat
    10681 gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc
    10741 tcctgttatt tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta
    10801 ctctacgata atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag
    10861 aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat
    10921 tctcgcaaac acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg
    10981 ttcggatata taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa
    11041 ccgctagtct ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc
    11101 tgtaaaatca gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata
    11161 actttccaat ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga
    11221 cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc
    11281 tggataacta tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg
    11341 aaaaacctca tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc
    11401 taacgcttga tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca
    11461 cagcgaatca gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat
    11521 attgccttcc acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat
    11581 caacatctct tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac
    11641 cgctaatgct ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg
    11701 acgtttcgaa aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat
    11761 gaaagcaaat gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg
    11821 agcatctgac catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg
    11881 tacgatcaca tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg
    11941 ttttacttca tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat
    12001 ttgacgttcg ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg
    12061 aacgtctaaa gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt
    12121 taattgttct ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct
    12181 acccagtaat tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa
    12241 tgggccaatg tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg
    12301 gctcgtgcat cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa
    12361 atgattttgt caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga
    12421 agaatctctg catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca
    12481 tgccagattg cattattata attcaataac tccggatcaa aaccgtctct tacgattacc
    12541 attttgaact cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta
    12601 gctttttcag gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca
    12661 agcacatgct tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct
    12721 ctagaataag cagtatattc attactatag tccatattca tacaaccctt cattttgaat
    12781 ataatcaatt actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg
    12841 acgtattttc gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg
    12901 agtatccgtt gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc
    12961 atcgatttta taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata
    13021 atctgtatcc ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc
    13081 accacgagct aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc
    13141 caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg
    13201 ataagaaggc atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat
    13261 cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt
    13321 cttctctaag aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc
    13381 ttgcgtattc atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata
    13441 cttttctttt gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc
    13501 gcaatctatt tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg
    13561 aagtaacgat actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat
    13621 tgctgaattg atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct
    13681 acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa
    13741 tgctttgcgt ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc
    13801 tgttacagtg atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa
    13861 ctcaaaacga acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa
    13921 tccacctaca tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt
    13981 tacatcattt gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct
    14041 tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat
    14101 ttcttttaac ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg
    14161 gcgatggata attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat
    14221 tttatccaag gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt
    14281 atttttgatg atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata
    14341 gacatcccgt ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga
    14401 tttcttagca ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc
    14461 cttcatccat tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt
    14521 cccaactaaa agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc
    14581 attgaagtca aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt
    14641 cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct tgggtctcat tataatggcg
    14701 taatctgaaa caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt
    14761 ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat
    14821 acattgtaat tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct
    14881 aatagatagg ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct
    14941 gttgcgataa tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata
    15001 tcagtcataa tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc
    15061 tttgctatgt tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca
    15121 aatttttcaa tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg
    15181 attcctgcat ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta
    15241 ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt
    15301 aattgggcag gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa
    15361 aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg
    15421 tattatactg catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag
    15481 catttcttta caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa
    15541 acaactaata ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc
    15601 gaacaagttc cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc
    15661 gtggcaactg ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc
    15721 gtagttctgg cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt
    15781 tccacaaaat cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca
    15841 tccagacttc atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga
    15901 tgatggcgat gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct
    15961 ctccttcgcc gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg
    16021 taatcaccgg tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc
    16081 cttcaggatg gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt
    16141 gaataccgat cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt
    16201 cgtcatctgc aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa
    16261 aaaaatccaa gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg
    16321 tcttatccac gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc
    16381 tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca
    16441 gccttttgga aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca
    16501 gcaaaaccga tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg
    16561 gcagttactc ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg
    16621 ctatgccgtt tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct
    16681 tgcatccgtg cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct
    16741 cgttcgaata atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa
    16801 ttaggatcca tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg
    16861 actgcttcat gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga
    16921 aatgctagcg gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca
    16981 ataatcaaag ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat
    17041 actttttctt caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc
    17101 cacatgttat caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc
    17161 cgaatatatt tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt
    17221 gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact
    17281 gcaggatgac taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata
    17341 aattcactta aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg
    17401 attatttttg caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca
    17461 ctgtctactc gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga
    17521 gaaggagcaa aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct
    17581 tggaatacta cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt
    17641 ttttttacta aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga
    17701 gtattcattt ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg
    17761 gcattttcat aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt
    17821 tttgtttgtg agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt
    17881 aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga
    17941 acttctatat ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat
    18001 gccgctgctg cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga
    18061 actttccgaa tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca
    18121 attctgtctg ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga
    18181 atgactggga catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca
    18241 tcgatcgttg cacttttagg accaataaat gtgatattgc actcttcaca catggaggca
    18301 aatcggctat tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg
    18361 attgcagcgc tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca
    18421 atgcagattg cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat
    18481 atcgctacgg tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca
    18541 ccgcggttag ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa
    18601 ttccgcttcg gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga
    18661 agctttcact ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt
    18721 acggaatttt gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag
    18781 agaaagtaat gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat
    18841 aacaggttct tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt
    18901 agcaacgatc cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata
    18961 acgatgtgga atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt
    19021 tgcttatttt tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc
    19081 tacaaggatt tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat
    19141 cgcttcaata atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc
    19201 tggtttgtcg ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat
    19261 tccatcaaat actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc
    19321 aggtatgaca ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat
    19381 gcctcgactt gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa
    19441 ttccgttagc gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa
    19501 aattattcct cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa
    19561 ttactcattg cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca
    19621 atctcaggat cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc
    19681 agcaaagttg ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat
    19741 ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt
    19801 gcttctgcct ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt
    19861 tccggctgta caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca
    19921 tctggagtag gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct
    19981 ccatagattt tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct
    20041 gctccttcac ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta
    20101 ggatcagttg attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa
    20161 atcgttgctt ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg
    20221 aatgcttccc caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata
    20281 ccttttgcac ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt
    20341 acgaacattg gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg
    20401 attgttggaa gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt
    20461 ttttctttat ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc
    20521 gaaaacatat ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct
    20581 ttgacttcac ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg
    20641 atgccattcg tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt
    20701 gaagtgactc catagccagt aattaccact cgattcattt gtttgctctc cttttcttat
    20761 ccgtgcatga ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt
    20821 gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt
    20881 tgcttttccg cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg
    20941 aatcctggcg caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat
    21001 tttgttaatc ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca
    21061 atcaacccag atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt
    21121 ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg
    21181 aaatcttctg catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact
    21241 aacacatgga tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca
    21301 taatcagaga tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct
    21361 atcttttctt ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca
    21421 aaagctaagg ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt
    21481 ccttttacat ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct
    21541 tattctcaac acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata
    21601 cttttccggg gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac
    21661 tctcatagaa acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt
    21721 tcttcattat ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa
    21781 tctgacttag gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag
    21841 gcccgctgac atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag
    21901 cttcgtctac tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg
    21961 caggagatac gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg
    22021 gcgcattcat cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag
    22081 cgcctctttt tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta
    22141 atgccgaata ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct
    22201 tcaataagcg ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct
    22261 gatccaaacg ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac
    22321 ttgcttcatc aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt
    22381 gatattgtgc accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat
    22441 ccagaatata tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa
    22501 tgtcccagcc cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact
    22561 aaatcttgaa ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca
    22621 atttcattga tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca
    22681 gtagtcaatt tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt
    22741 ttttcgaata ccataataat atattcctcc gatattgttt gctttttatt tttataattt
    22801 aacagtctac aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa
    22861 tattactttc tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa
    22921 gatagggatg cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc
    22981 ttgcggcatc tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa
    23041 aagcagataa tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg
    23101 tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc
    23161 ttctgaatag aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt
    23221 accgtcagct gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat
    23281 cccacctgct ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt
    23341 agatagtgtt tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc
    23401 agccatgctt aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc
    23461 atgagttgct cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg
    23521 cgacattgtt gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat
    23581 caatttttcc gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg
    23641 ttcacgaata cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag
    23701 atcattatta gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa
    23761 ttgttccatt aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc
    23821 atccagcaag gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc
    23881 acgatgaaaa tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt
    23941 tacaacgcgg cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt
    24001 gttaatagca gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt
    24061 tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc
    24121 tttgaattga gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact
    24181 aacaagataa tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag
    24241 tttgattatc aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct
    24301 taaaagatc
//
LOCUS       DAZUKD010000036        23777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000036 DAZUKD010000000
VERSION     DAZUKD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.037"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..648)
                     /locus_tag="ACG8N9_001203"
     CDS             complement(37..648)
                     /locus_tag="ACG8N9_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4896817.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(771..895)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(977..1693)
                     /locus_tag="ACG8N9_001204"
     CDS             complement(977..1693)
                     /locus_tag="ACG8N9_001204"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4896818.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1699..2268)
                     /gene="scpB"
                     /locus_tag="ACG8N9_001205"
     CDS             complement(1699..2268)
                     /gene="scpB"
                     /locus_tag="ACG8N9_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4896819.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            complement(2252..3022)
                     /locus_tag="ACG8N9_001206"
     CDS             complement(2252..3022)
                     /locus_tag="ACG8N9_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4896820.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3019..3390)
                     /locus_tag="ACG8N9_001207"
     CDS             complement(3019..3390)
                     /locus_tag="ACG8N9_001207"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896821.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3409..4296)
                     /gene="xerD"
                     /locus_tag="ACG8N9_001208"
     CDS             complement(3409..4296)
                     /gene="xerD"
                     /locus_tag="ACG8N9_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4896822.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4392..4865)
                     /locus_tag="ACG8N9_001209"
     CDS             complement(4392..4865)
                     /locus_tag="ACG8N9_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4896823.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4970..5833)
                     /locus_tag="ACG8N9_001210"
     CDS             complement(4970..5833)
                     /locus_tag="ACG8N9_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4896824.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6029..8182)
                     /locus_tag="ACG8N9_001211"
     CDS             complement(6029..8182)
                     /locus_tag="ACG8N9_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4896825.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDTEKDISGVNAAGKESLKTNDLK
                     TNDLKTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAET
                     QSASEDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKK
                     SKKVPYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDH
                     HQFIRADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAI
                     VDDHLADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKD
                     GKVVDSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEE
                     AKKAEKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKS
                     ARASDIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYAT
                     STKSALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            complement(8399..9508)
                     /gene="rpoD"
                     /locus_tag="ACG8N9_001212"
     CDS             complement(8399..9508)
                     /gene="rpoD"
                     /locus_tag="ACG8N9_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4896826.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9532..11373)
                     /gene="dnaG"
                     /locus_tag="ACG8N9_001213"
     CDS             complement(9532..11373)
                     /gene="dnaG"
                     /locus_tag="ACG8N9_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4896827.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11555..13870)
                     /locus_tag="ACG8N9_001214"
     CDS             complement(11555..13870)
                     /locus_tag="ACG8N9_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4896828.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14055..14915)
                     /locus_tag="ACG8N9_001215"
     CDS             complement(14055..14915)
                     /locus_tag="ACG8N9_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4896829.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14974..15606)
                     /locus_tag="ACG8N9_001216"
     CDS             complement(14974..15606)
                     /locus_tag="ACG8N9_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4896830.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            15773..16534
                     /locus_tag="ACG8N9_001217"
     CDS             15773..16534
                     /locus_tag="ACG8N9_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4896831.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16560..19151
                     /gene="mprF"
                     /locus_tag="ACG8N9_001218"
     CDS             16560..19151
                     /gene="mprF"
                     /locus_tag="ACG8N9_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4896832.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19339..20526)
                     /gene="tuf"
                     /locus_tag="ACG8N9_001219"
     CDS             complement(19339..20526)
                     /gene="tuf"
                     /locus_tag="ACG8N9_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4896833.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20894..21250
                     /locus_tag="ACG8N9_001220"
     CDS             20894..21250
                     /locus_tag="ACG8N9_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4896834.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21491..22495)
                     /locus_tag="ACG8N9_001221"
     CDS             complement(21491..22495)
                     /locus_tag="ACG8N9_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4896835.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22661..23512
                     /locus_tag="ACG8N9_001222"
     CDS             22661..23512
                     /locus_tag="ACG8N9_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4896836.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23529..23774
                     /locus_tag="ACG8N9_001223"
     CDS             23529..23774
                     /locus_tag="ACG8N9_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896837.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         6586 a         4976 c         4162 g         8053 t
ORIGIN      
        1 aaaaaagacc aagaaatttc ttgatctttt tgataattat ttgactgctg tatgacgttg
       61 ttcgaactga tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa
      121 gacagcgctc acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata
      181 gtttgctaaa gacatgccta gcatctgtgt tgcgttcagt cctaaaaaca ttaagtaaag
      241 aggcgtaata acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt
      301 aatgacacct aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata
      361 aatcgggaat gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc
      421 cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc
      481 ggccgctgga ccgaataaga acatgctgat caataccggg atatcactaa aatcgatctt
      541 caaaaaacta aaagccggca tgatcggaaa agcaaaatac tgtaaaacta acgcgatcgc
      601 agcaaacatc gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc
      661 tgttaggact cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt
      721 ttccagggaa aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc
      781 tttatccaga ctatactgtc ggtatcggaa tttcaccgat tcagctattt tttgcaaaat
      841 aggtcgtgga ctataaccac cggtcgggaa tttcaccctg cccctgaaga cgaaccttat
      901 ataatttttg tttacatatt cattatacat atctattcaa aaaacgcaac atctaacact
      961 tataaaaagt aagtgttcat tttgagtggt tcagcaaact gctgacttct tttttgttca
     1021 gttcacggta atctccagga cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt
     1081 ttagtttttg cacaggaaga ccaactgctt gaagcatatt tttcacttga tgattacgtc
     1141 cttcgtggat cgtcaattcc acgatactgt ggtttgtctt aggatccact gataaaattt
     1201 ggaatttagc tggagatgtt tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa
     1261 gcatcgattt tgtagcgatc ccttttacct ttgcaacgta aactttatcc acttcatgtt
     1321 ttggatgtgt caggcgctga gcaaaatctc catcatttgt cagtagcaag atccccgaag
     1381 tatcgtaatc cagtcgacct acaggataga tacgttcttt taccatcggc aaaagatccg
     1441 taacaacttt tcttcctttg tcatctgcaa cagcagaaat aactccttta ggtttgtaca
     1501 ataaataata accgtattct tcttgataaa ttgggacttc atccacttcc acagtgtcat
     1561 gtttcccaac ttttatccct aattccctta caacttgtcc gtttactttt acacgtcctg
     1621 caaggatgaa ttcttctgct tttcgacgag aagtgatgcc agcgtgggca attactttct
     1681 gcaatctttc cattacaatt attctccttt attcattcca tcaaagaata gatctggtga
     1741 cacgtcttct tcaaatgaaa tttccatttc ttgtgtatca ggcagttctt ctaatgattt
     1801 caaaccaaaa taatctaaga agtagccagt tgttccatat aatatcggcc gacctggccc
     1861 ttctactcta cctttttctt caatcagttt atgggcagct agacgctgta ccgctcctga
     1921 aacctgaact cctcgaatct gctcgatttc agctcttgtg attggctgtt tataagcgat
     1981 gattgctaag gtttcgacag cggcttgaga taatgtgttt gatgtccctt gcgcatatcg
     2041 cttaagtaag ggagccagct cttttttagt cgttaataca taatggcttt ccgtttcaaa
     2101 aatatgcaaa gctgagtgcg agttttgagt gtaagattct tttaattctt tcactaaact
     2161 tgtgacttct gcttcgctga gatttaacag tccgcttaat tcttcattac tgatcccttc
     2221 ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc
     2281 ctactgctgg ataaagcata attgattcat agttttcttt ttgttgcgca atgatctgcc
     2341 cttttttcat caattctagc aaagccataa aagtagtgac aacttcactt ttagaaggtg
     2401 actcaaaaaa agaatcgaaa gtacagccat gcgcatttct ttttatacgc tcttcgataa
     2461 ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat cgttttttcc actttttgag
     2521 aaaatttctt acgttctaag attttctgca tggcacggat caaatcgatt tttttgactt
     2581 gtcctcgctt caacggcttt tcagcatccg ctaacccatc gatgttcata ggttcttttg
     2641 tgtaataaag acttcgttcc tctgcttttt ctgtcagtct ttcagctgca tatttatatt
     2701 tacggtattc taaaagctga gtgaccaatt cttctcgagg atcttcttcc cataattctt
     2761 catcatctgt gacgaattct tggacaggaa gcaatgtctt gcttttgatt gccatcaatg
     2821 tagcagccat cactaagtat tctcctgcaa cagctagctc tagtgttttc attgtatgga
     2881 tataattcat atattgctcc gtgatcgtag cgatcggtat atcatatata tcgatttcca
     2941 tttgctgaat cagatgaagc agcagatcga gaggaccttc gaatacatct aatttaatat
     3001 tgatctccgt caactcattc actctttctt ctttttcttt gctagccatt ttccaatgta
     3061 aggctctgtg atttcagttg cacggatgga gacatccggg tacttttcct gtaattcatc
     3121 taacatttga aagcccatat tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat
     3181 gatcagctga tcgttcgttg cccagtaaac accaataatc ccttgaatgt tggttgaacc
     3241 ttctggcgta aagaaaaaaa gttcataatc gtcttcagat tcgtacgtat caatctcctt
     3301 caacaaagaa gttttacctg tcagtttctt atggaaactc aatagtccca tagcaatttt
     3361 acggttttct ttccggtaat gtgttaacat cttctcacct tcctatgact aagcccttgg
     3421 aaaatgctgc ttataaacat ccgtcattct tttcttcgtg atatgtgtat aaatctgagt
     3481 cgtagagata tccgcgtgac ctaacaattc ctgtactgtc cgcaaatcag ctccattttc
     3541 tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt gttacttctt tgtaaatccc
     3601 agcctctcgt actagctgct tcagattttt ccagattcct tgccgagaaa gacctttccc
     3661 atgattattg acaaatacat gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc
     3721 gtctaagtag cgttccagcc attggatcgc ataatcacct aaaggaatga tccgctcttt
     3781 atctcctttt ccaactgtct gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa
     3841 tccaatcaat tcactgacgc gcatccctgt agcatacatg acttccaaga ttgcacgatc
     3901 tcttatccct aacgtttttg tcgtatcagg tgcttcaatc agcttttcta cttctgtcag
     3961 agtcaatgta cttggtaatt tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc
     4021 atgatcagtg atacgctctt gccgtaaaaa ctgatggaat cttctgagac ttgagatcat
     4081 ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac ttctccagaa aacggatgac
     4141 taaaaagcga tcgacatcct gccatgaagc aattttctgc tcctgtagaa aatgcaagta
     4201 ctgttccaaa tcacgttcat agctttgtcg tgtattggcg gacaatcccc gttcaatatt
     4261 caagtagtgg agataatcgt tgatttgctc tttcataata atccccctca tgcctctcta
     4321 tcatatcaga taccgcttca ttctgatagt ctttttccat aatctgagca aagaaaaagc
     4381 cgtctagaca ctcagacgga ctggtttctt ttttcttttt catagcattc ctgacagatt
     4441 ccatggaacg ttagccgatg atcttttact aaaaaatgat aacgttcttc tactacttgc
     4501 tccacttcaa gtaaaagatc ctcttctact tcctcgatcg tgccgcagtt caagcatagt
     4561 aaatgatgat ggaaatgttt ggcgccttct tttctcaaat cataacgggc taatccatca
     4621 ttaaaactta ctttatcgac aattttcaat tctgtcaaga tttcaagggt tcgataaaca
     4681 gtagcaagtc caatttccgg actctttttt ttcactaaaa agaagatttc ttctgcagat
     4741 aaatgctctt tctcattttc caaaagcaca agcagagtag cttctctttg cggtgtcagc
     4801 ttaaaccccg cttcatgtag ctgctgtttt gtttttttca tcgccgaaat agcattcatg
     4861 accatttact caaccccaac aatttataat aattacaaat aaaaaaagat ataacactat
     4921 gttaattaaa attattataa gctagttaag aaaaacatgc aagggattct caattagatt
     4981 ttttgactag ttttgtcaca ccgttttctt gtgtaatcaa tccttgtttc aaaagattac
     5041 ctaatgcgcg cttaaatgct cctttgctga taccaaaagc tttcatgatt tcttctggat
     5101 ccgatttatc tgtaaagttg atttgctgat ctgcagaacg ttctaaaatg gttaaaatca
     5161 tttgtgcatc atctgatata gcttcatatg ctcttggctt caatgaaaga ttcagtacac
     5221 cgtctggtcg gacaccaatc actcgacctt ttatttgctc gcctagacga ggttcattat
     5281 atcgttcaga aggatggata aacccaagat agaaatcttc cgttaaaacg tacgtacctg
     5341 ttaatttcaa gcggtatgct gttcctatca catctttgtt tttcatttct tctgttccat
     5401 atttgcttaa agatttgaat attttttcat ctgctaaact gccccaaata cggtcttttg
     5461 catcaactct aagggaaatc atcaagcgat cccctttttt aggccatagc tctgtcattg
     5521 tcggcaattc atcaagagat acgacaaagt ctttatctgg aagaccgata tcgacaaacg
     5581 cacctaaatc ttttcgtgta gcagtaacga ttccaaaagc gtaatgaccg atacggcttt
     5641 taggaatttg tgtagtgatc cgattttcat gtttttgatt ttggtagccg aatccttcca
     5701 ctgcttcacc gattttgtgt tcaccttctg atttgcttaa gccaaaggtt tgtccgtttt
     5761 tttggacaaa gtagtttttt tcattttcat caataataat acctgtaaat atagttgcta
     5821 ataaatcgtt catttttttc tccctctata gtgaaaggtt ttcttaatac atttctttgc
     5881 tatcataaca taatgttatc tacttgaata gctgagcctt ggtttggaaa ggtgaaaaaa
     5941 ggagtatgac aacagttgtg tcatactccc ctttttcgaa taaacggtgt taaacggcag
     6001 gctcacagcc tctttcattt gcctaaaatt aattgctccc atttcctccg aaccatccag
     6061 ttttacagtt gatggttacg atgtaaaatg gcaaagcact ttctccaatg cctttcatca
     6121 atgcagattt tgttgaagtg gcatatagat tgtaatagcc ttccccattt tcgattctgg
     6181 ctttttctaa tctgtatcct ccttcaacga tatatccacg ttggatacat gtagcaatca
     6241 ctttgctttg gacacctggt gcccacgtcc atgctggatt agatgcatcg gcaatatcac
     6301 ttgctctggc agatttgatt ggctgattcg ctgagtttga agaggaaggg gtcgaggatg
     6361 ccgcttcttg ctgtgctgct tgtgcgaccg cagcttgttg agcggcttga gctgcagctg
     6421 ctgcttgagc ggcttgtttt gcttgttctt cagctgcttt tttcgctttt tctgcttttt
     6481 tcgcttcttc tgcttttttc ttttcctctt cttctttttt ctctttatca gtgagcatct
     6541 tatctacttg ttgcaattgt tcattcaact gagcagatag atctgtattt ttcacttttt
     6601 ctactgcttc ttttgccgtc ttgtattggt cgcgtgttgc tgaatccact acttttccgt
     6661 ctttaaagac tatagctgtt ttttctttag ctgtttgcaa ttcagcatat tgatttttag
     6721 cttctgtttg tgcttgttga atcaattttc caaaatctgt gttatccgtt tcgattgctt
     6781 ggatctcttt atctgctttc aacttgatgt catctgctag atgatcatcg acgatggcag
     6841 gtgctatgaa ccactcattc actttttgga tatctcggat cttagattgg atatcttggt
     6901 aagttgtttc taactctgaa tatccttttt cattcttaag actggataaa tcatttttta
     6961 gttttgataa atcttgatga atcatatctg cacgaataaa ttgatggtga tcatctgtat
     7021 aaaaggcagc aagtttctca tgaagttctt ccatttgttg ctgtcttttt gcttctgctg
     7081 ctacttcggc ttctactttt tgatgatggt cataataagc ccagcctccc ccacctagta
     7141 gaagaactgc tgctaaagcg agatacggca ctttttttga ttttttcttt tgtgtcttat
     7201 ctaaatcttt tttttctggg tttgattctt gaggcgtttt ttcagtattt ggcacgattg
     7261 ttattggttc tgtttctgct tcaatcagct caggctggtt ctcttctttt tctattttag
     7321 cttcaagagc agtgctatct tcacttgcag attgagtttc agcatcggct tcattattta
     7381 ttccatccga ctctgttgcc ttcttgtctg ctttttcgag catttctttt ttatctgctt
     7441 ccggcaaccc attcgatttc tcgtcttctg attgtttttc agacaatgtt tcagtttttt
     7501 ccgggatatc cgtttttaga tcattcgttt ttagatcatt cgtttttaat gattctttac
     7561 ctgcagcgtt tactccacta atgtcttttt cagtgtcttt ttcagtgtct ttttcagctt
     7621 catgttctgg ctctactttg ttttcctgtg caattttttc actgatcatt ttgagcagct
     7681 ctttcgcgtg ctcttctttt gtatcttcac gatgtgcttt gatataagcg gatagtatcg
     7741 aattttcttc ctcattatcg acgatttctt tcagttcagc tactcttctt ttttgttttg
     7801 attcaagcgt ttcttcttga ttctttcctg attcttccga agtctcatga acagttgctt
     7861 tcttctgttc atttttctct atattagtta cttgatcgtg agcaggtggt tcttcgtctt
     7921 ctggttctgc tttttcgaat aattccatta cagattctag aggtaaatct ttcaattccg
     7981 accattcgat tggatcgttt aattctgtat caggaaagac tgcatcttca gttgatttat
     8041 cctgcatagc tgcatctgta tttgtttctt caattgattc ttttatttgc tgatcttctg
     8101 cagttatttg atgaccgcag tctggacaga tagtattctg ctccattttt ttcgatccgc
     8161 aatttggaca ttttttcatc acttagcgat cggctccttt actaggttta cgctaccatt
     8221 taatctatca tattctatac gtcacgccaa tagcttattt ttaggattta cttaaaaata
     8281 atgaatgaat ttccttccta tagaaaaaaa agcttgagac tgcgtagtct caagcttttt
     8341 ttcttttaaa ataaaccaaa cagttttact gatttctctt ctggtcaata gttcatgctt
     8401 attctaagaa atcttttaat tgttttgaac gagaaggatg gcgcaatttt cttaatgctt
     8461 tagcctcgat ctgacgaata cgttcccgag taacaccaaa tactttccct acttcttcca
     8521 atgttctcgt ccggccatca tctaaaccaa aacgtaagcg aaggacattt tcctcccggt
     8581 cagtcaatgt atctagtaca tcttccaatt gttcttttag taactcataa gctgcgtgtt
     8641 ctgctggact tgttgcttcc tgatcttcta tgaaatcacc taagtgagag tcatcttctt
     8701 caccgattgg cgtttcaagt gaaacaggtt cttgcgcgat tttcaagatt tctcggactt
     8761 tttctgttgg aagatccatt tccgcaccga tttcttctgg cgtaggttct cttccaaggt
     8821 cttgcagcaa ttgacgttgg atacgtatca atttattaat tgtttcaacc atatgaactg
     8881 gaatacggat cgtacgtgct tggtcagcga ttgctcgagt gatggcttga cgaatccacc
     8941 atgtagcata ggttgaaaac ttaaatcctt tgcggtagtc aaatttttca actgccttca
     9001 tcagtcccat gtttccttct tggatcaagt caaggaactg catgcctcgt cctacataac
     9061 gtttagcgat acttactact aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt
     9121 ggtcgccttc ttcgattttc aatgctaacg ctacttcttc ttccgcagtt aaaagggaga
     9181 cacggccaat ttctttcaga tacatacgaa ccgggtcatt gatttttaca ccggttggcg
     9241 cagatagatc ttctgttttt gcttgttcag cttttttctc agcactcttc aagctgtgaa
     9301 ttgatggatc accattttca tccaccacac tgatacctgc gtcttcaatc ttttggatca
     9361 atttgtccat ttcatctgca ttcaaagtga atggtgtagc aagctgattg gataaatcat
     9421 catatacaac ttgtcgtttg ggtttgtttt ctttgatgaa agctgctact gctgcttcat
     9481 acgttttttt atctgttcca tttgccataa aagaaggccc cccttgagta gctatttggc
     9541 ttgttttaac tgtttcgtta aattgatgat ttcaatcgtc aattcaagct caagctgttt
     9601 gtttccagtt cgtctggctt cttgctgcat ttgttttttt aatgttattt catcagctaa
     9661 gccagatttg ctgatcacat ctagcaagtc ttggatttct cgtggcgaac tttcttctgc
     9721 caccggtaaa tattcgattt ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc
     9781 taaaaactct gcaaggttga attcataatg ttgtgagaca tatgcatcga ataaaaaata
     9841 aacttctgca tatgcatcat gaataaactg aaattctcgt tcttttaaaa tattacggac
     9901 agaaacttct ttaaatgcac gatataatag aatctgctct gccttttcta cttgcgtcaa
     9961 aggtctcttt ttccgtgcag tttcttgtct cgtaacctga gtgtgttgac tttcctgagg
    10021 ttcttgacga ttttgctgat ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc
    10081 tctgccgatc tgaaactctg ttgacagttg agatagatac atatcttgtt cgattggtga
    10141 aacaactcgg ctaagttcaa ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt
    10201 atctaaattt ttcccttgtt ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc
    10261 atgagccact aattcttgga aagatgcttc tccatactta cggagatatt catcaggatc
    10321 aagcttttct ggaacgacta cgacagaaag tgctaagcga ctgttttgcc gaagcaattc
    10381 aatccctcga ttggttgctt caaaacctgc gttgtctccg tcatagcaaa agaccaattc
    10441 tttcgtcagc ttttccattt gttggatctg ttgagctgtt agacttgtcc ccatagaagc
    10501 taatccagtt ttgataccag cctgccatgc agcaatcaca tccataaacc cttcgaacaa
    10561 caaaatttct ccgtttttcc ggatatcgct tcttgcttta tcaagattaa ataaaacttg
    10621 tcttttattg aacagttctg tttctggact attcaagtat ttgggctgat ctttttcctg
    10681 ccctttttca ggtgcaagcc agcgtcctga aaaaccgatc gtctggcctt gagaattacg
    10741 aagtggaaac atcactcttt gataaaaacg atcagacata cggccatcat catgcgtgac
    10801 aaacaatcca gagcgttcta acaattcatt tgaaattgat tcattcttga atacttgctg
    10861 aagaaaagaa cgctcattag gtgcaaaacc tatgttgaat tcttcaatta attctaaaga
    10921 caacccacga ctttgcaaat attctaaagc tggctgtcca gcttttgtat gcaatagcat
    10981 gtgatgatag atttccgctg ctttttcatg agcagcaatc agtttccccg tagcagattc
    11041 aggattttgg acagctatag tacgctgacg ccattgatct tctaacggta tctgttcgaa
    11101 gtctgccact tttaccactg cttctggaaa acttagacct tctagttctt gaagaaaagt
    11161 aaacacgttt ccgcccttac cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac
    11221 agaaaaagac ggtgtacgtt cttcatgaaa aggacacagc cccaagtaat ttttaccaga
    11281 ctttttcaac tggacatatt gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt
    11341 ttcgataacc tcttgaggaa tccgttgagc catttttcca ccccctgctc tattgatatc
    11401 ttttgggttt aacagaagat atttcaggag aataaccggt aaaaaatcgc actaactgaa
    11461 atcagtgcga ttttaaagga cacaattata catgtttgat tgtatcatat ttcattgctt
    11521 tttcaagtat aacacatttc tatctcatta agcatcaatt tgctactctt ttcagtaact
    11581 gattttgaat atcagccttc cagtacaata ctcgaagaac aatatttacg agcatgaata
    11641 caaagaagta tcctacgctc tctcccaacc ctaagtttac ttccaaaatt ccatggaaca
    11701 gcattgcagc tacatatatc cctacgatcg tcgttactgc taggaagatg cgcaatgggt
    11761 tgaagggata acaagctttt acaactgcca tgcaactgat cccaatcagc aagtaataca
    11821 tcaaagtagt agtttcagca tttccccatg cgaatgtttt tccaagtaga tacaccgcaa
    11881 tgatattcac tactacaagc aaggcatttg gtaaagcatt aataagtgct gtaggcaaat
    11941 aacggtaagt gatttttctt ttatcttgtt caaaagataa aaagaaggaa ggataccctt
    12001 caatcgctaa atcaatcagc gtgatttgga tagggataaa cggaaatcca attgctgtaa
    12061 cggcacatat aatcgataaa ataaatgaat aaatcgtttt gataaaaaag atgcctgaga
    12121 ctttcgtcac attgttgacc actcggcgtc cttcaaacag gacttctggc aatgtagtaa
    12181 aatcagagtc caacaatacc agattcgcaa tctgacgtgt tgctccatca ccttctgcca
    12241 tagcaatgct acaatctgct tctcttaacg ccaaaacatc attcacaccg tcgcctgtca
    12301 ttgcaaccgt tctgccgctt tctttcaatt catttaccag caatttcttt tgctgaggac
    12361 tcactcgtcc aaaaaccgta tagcgatgag cagcttctcg cacttccatt tcttcagtaa
    12421 tttgagaaag atcgacatac gcatcataat tcggtaatcc tgcacgtcta gcgatattgg
    12481 agacagtaaa aggattgtct ccagaaatca ctttcagatc cacaccttcc tcatgaagat
    12541 aagctagtgt ttcttgagcg ttttttcgga ttggatcatc gatttcaaac agagcaaccg
    12601 gctcaacaaa tgctggtttt tcttcgttca attgctgatt tggagcgatc cctaacataa
    12661 gtacgcgata gccattcgct tgggctgttt ttagactgtc cggaagccga tcttcttcca
    12721 ccaaacgttc aggcgctcct acgtacacga ctcccagttc tggaaattcc atcgcgcccc
    12781 atttgcgatc agaagaaaat gcgactttgt ttgtcacatc ataccgatta gaaagctcaa
    12841 aataatctct catagcccgc atagtgatgt tgttatcact gctttctgcc agatacgtcc
    12901 ccataatttc tgggatggct ttctcgtaca gcggatgtag tgcttctacc cgctggatac
    12961 tcatttttcc ttcagtaatg gtccctgttt tatccaagca taatgtatcg acatgggcca
    13021 gcgtttcaat tgaatacatg tcttgaacaa ggattcgttt ttttgccagt ttggttaccc
    13081 ctgtcgttaa tgcaatactt attagcaata ccaaaccctt tggaagcatg cctaacaaag
    13141 ctgctgcaga tgcaataaca gaaattttga tagaagcatc gcggatgaac agtgcttcca
    13201 aaaatagtat gatccccaaa ggaataatca cgaaactagt gaatttggat actttgcgaa
    13261 tcgaatctac aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga
    13321 cagcataatt atctgcacct actcggataa cttcagccat gacagaaccg cttgtcaaaa
    13381 agcttccaga aagcaattca gctccagttt ctttgacaat caaatcagat tcgcctgtca
    13441 aaagagcttc atttgcttct gcccgtccag atataacctg taaatcggct ggtatctgtt
    13501 ctcctgctcc gatttttacc aaatctccta ataccagctc tttagtagaa atcgattgtt
    13561 ctttaccgtc ccggatcact agtacttctg attgggaaac aatcgataat ttccgtacaa
    13621 gatttcttgc atgtatttct tgataaattc caataacgat attgacgaca ataattgcta
    13681 aatacgccat gttggaatac gcacctacaa ataacagaca aatcccaata ccaaagttca
    13741 gaaaattgaa taacgtcaat atattatcgc tgaatatttg ttttgtactt tttgatgcat
    13801 cttcctggta gtcattttgc tggccagaag ccattttctg aactacctct tgctgtgtaa
    13861 gtccattcat tttctttttc tccattttcc gccaactttc aaaaataatc acgtgtttaa
    13921 gtctagcgtg ttccaagatt aaaaacaact tgtttttata aaatttatag aaaaacacac
    13981 gaatgatttt acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt gctgttttta
    14041 ttatcaatct atctttactt atattgttcc tccaaacggc tcatccacca acctagtgct
    14101 gtaccggcta taaaaagaac aggacaagct agtaccccag caactcccaa tcccgcgtaa
    14161 tcggtaggaa tgatcataat cgttgaagca aaaactacac ctaaaatgaa atggaatagc
    14221 ttgctatatg cctttgagaa aatgtaatcc atgatttttg acaatccaag aacacaaaga
    14281 atcccaccaa ttccgatcgg cacaatcact tcaagtcgta cttctttaaa tccgtcagcc
    14341 atcgctttat acaatcccat atacaccaaa aaattagaag ggctcaatcc tgggacgatc
    14401 atacccaaac caatcaatcc gccggcaatc agccatgttc caaaattctg atcgacttgc
    14461 ccgtcaaata attgagcgcc gtataataaa aacaaataag ccagaacaaa agtaacaacc
    14521 atgatgatca catcagaagt atctcttcct tttttccctg cttctttcca taacgcagga
    14581 acagttccag cgatacatcc gacaaaaaac cataatatcg ttgtttcata cgcacctaat
    14641 aaaaaactga ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag accaacaggc
    14701 agaaaaaaca aaacattttc tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc
    14761 cgctcataca tgccaaagac agctgctaat gcaccaccgc tgactcctgg caagataaat
    14821 cctgaaccga taaacattcc cttcacaaac cgtaacatcc agtctttggt tgtgtacccc
    14881 actttttgat tttccatttt ttgccttttt tccactattt gcgcactcct tctttcattt
    14941 aattttattc atgattgaac atttcctgct attttactta aaaacttttt cataggtctc
    15001 tattattttt tcattcattt tgacaatatc aggataaaac atgttacctc cattatgata
    15061 gagatagccg ccattgtaaa gcaaggactg gatccgccaa tatcggtatt gtttcccttc
    15121 ctcatttcct aaaagtgggc tcagcacatc tttagaatac gcttctgcta attctactgt
    15181 gttttctccg ccatgatttt ttacataggc aatataatct ttcccaaaat tatatgcttg
    15241 gacagctgtc cataaatcgc atccttgttt ctttgcttct actatcattt ccgccaaata
    15301 tttgacacct tgatcaatac tgtcagctgg attatcaatg atctcagctt ctccatgcag
    15361 actttccgag ctccgcatga gatctgtccc ttttcctttt gattcggtca atattatcgc
    15421 cttgatcaaa ttctcgtagt tttccatatg ataatttttt gcagctgttg ttatttctat
    15481 ttgatagctt tccactcgtt ttaccgaacg ataagttctt cctcctaaaa acaacaccgc
    15541 accaataacc gtcagaatga tcatgtaaaa aagaatacgg ataatagata atcgttttcg
    15601 cttcatttca tcacctagtt attttgacat ctttttttat cttactattt ctttatcttc
    15661 ttggctattt cttccttgat ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt
    15721 ttttgtcatc ttacgaaaat gatcgttgtt tttcagtaaa aacaattgcc caatgactga
    15781 tataatcgtt atactttaca cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa
    15841 aatgccagat gggagcgaaa tttactatga aaagagcggt caaggcttcc ctctcttttt
    15901 actgcatggc aatgacggca gcggccgctt tttttcagaa caggtccctg tactcgaacg
    15961 ctactatacc gtgtatctag tagatagccg aggacatggt cgttcgacaa acgaagcatc
    16021 tatgttgaat tttcagttaa tggccgagga tctgaatacg atcatgttgc tggaaaaaat
    16081 cgatcaggca gattttcttg gatttagtga tggagcaaat ctagcactgg tatttgccag
    16141 cagcttccct aaaaaagttc atcgattgat tttaaattct ggaaacacgc ttgttaaagg
    16201 cgttcgtttt tctgccagag tcatcagtaa tattcattat gcatgggtat ggttactctc
    16261 tttatttcgc ccaagtcttc gcaaaaatct tttagttatc aaacttttgc tgcatgacat
    16321 cgggcttaca gaaaatgatt taaaaaaaat caactccccc actttgatca tcgtgggaaa
    16381 aaaagatgta atcaagctaa aacattcttt gtatatagct aaaactatac caaaagcttc
    16441 ctttgtgctt gtgaaagaac aaggacatga attagctaga aaagatcctg aacgcttcaa
    16501 tcgagaagtt cttcaatttc tttctgaaac ataactatta ggaatagaag gtgatttatt
    16561 tgttaaaaaa taccatacaa tggctgaaaa accacctagg actgttcaaa acaatctttt
    16621 tgatttctgt cattgtcatt attgtcggtg aattgatgtc catcggtaaa actctatcta
    16681 tccagcaatt aggagaaacg tttgccacta tacctgtatg gaaaagcgga cttatgctgt
    16741 tgattggtct tgtctctgtg ttacctatga ttggttatga tattattttg aatcggatgc
    16801 ttgaacaaaa gcagaatcct cgttacttgt ttgagacaag ctggctgatc aatacaatca
    16861 acaatattgc cggtttcggc gggtttgtta gtgtggggct tcgctctgaa ctatatggac
    16921 aaaagaaaga gagcagaaac gtgatccaag cattatcgaa agttttcttg tttttgatgg
    16981 ctggactttc tatttacagt ctgctttctt ttttacttgt agtcttcacg ccagttgctc
    17041 cttttttgaa gcaatattgg atatggttga tcggcggcgg tctatatttt cctgtcgtct
    17101 ttttatttac tactttcaaa aagaaaggat tccttggtgg tacctcactt aaaactcaag
    17161 gacagctatt gctagtttca ttgttggaat ggaccggtgt cctgtttagt ttcctttctg
    17221 ttggttattt aatggaaatc cggattgatc tgtggcaaac aattccgtta ttcattgctg
    17281 cttcagtgat aggaatcgtg tctatgattc caggagaaat tgggagtttc gatgtaatga
    17341 tgattatcgg gttgtccgct attggtgtgc caagagaaat agtcgttgtg tggatcttgc
    17401 tctatcgcct gttctactac atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa
    17461 atatcggttc cacttttgat cagcgttatt ctggtatccc taaacaatta gcaacagaaa
    17521 ttgcgcataa aatcgtggtt gtcttattat acttttctgg aatcatgcta gtactttctg
    17581 ccactatccc gcaagctttt acagagtttc gctggcttca tagtttgaac ccattgaaat
    17641 ttcattttat tatccaattt cctagtatac tattgggatt tcttttaatc attatgggca
    17701 gagggatcgc agcacgtgtg aaacgagcat atttgcctac aatcttcttg attgttctag
    17761 ctttacttta tgttttatta agtgatttta gtttcacacc ggtggttttc ttgtctattc
    17821 tgcttctgat cattcttgct tctaaaaacg aattatttcg ggaacaattg atctattcat
    17881 gggagtggcg tacgatcgat ggaatcatta ttggtgcact gtccttgctt tacatcgtta
    17941 ttggggtata caatctgccg gatttccctc atcggcgtca ccattttatt tcctttttct
    18001 tatttccttc tgaaaagatt tggttttcgg gattgttggc catcatcgct gttagtttca
    18061 tgatcgttct atttgttcat ttccttcaag gagaaaagaa acagattggc gaagccttta
    18121 atgaagaaaa ggccctgaag atccttacaa cttatggagg aaactccgat agtcaattga
    18181 tcttcttaaa agataagcgg atgtttgcct atgaaaaaga cggtgaacca actgttcttc
    18241 tccaattcgc ttgcttcaat aataaatgta ttgtaatggg tgatccttct ggtaagaaag
    18301 aagattttcc agaagcaatc gaagctttta tcgaagaaac agatcgacta tgctatttac
    18361 cagttttcta cgaaacaagc gaagagattg ttatgatttt gcatgaattc ggctacgatt
    18421 tcattaagat gggggaagaa gcttatgtag acctgaatag ttttacaact tcagggaaaa
    18481 aaatgaaggg cactcgtgct gtcttgaatc ggatcgaacg agaaggattt acatttgatg
    18541 tcttacaacc accattttct actgagcaaa tgagtacttt caaaaatatc tctgataatt
    18601 ggttgggttc acgaaaggaa aaagggtttt ctttaggctt tttctctgaa gattatctac
    18661 aaagagcgcc tattgcagtt gtcaaaaata cccaagaaga aattgtcgct tttgcaacga
    18721 tcatgcctac ttacacgaac aataaagtag gaacgattga tctgatgcga tatgatccta
    18781 aaaaagcacc ttctggcagt atggatttct tgttcttgca tttgtttgcc tatatgaaag
    18841 aagaacatat ccaatggttc aatttaggga tggcccctct ttcaaatgtc ggaacttcca
    18901 gaaagagttt tttacaggaa aggattgcat acttggttta cgagtttggt tctcattttt
    18961 attctttcca tggattaaga gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt
    19021 atactctata ttctagagat agttggattg catatgttat gattgctttg ctgattattg
    19081 ataatgcgcc agttgagcag acacaaggta gaataagtgg tttgaaaaaa tggatacgaa
    19141 gaaagtatta aaaactgcat accgatagat gaacactctc atgatgaata aaagagactg
    19201 aagtaaaacg aaagttcatg cttctacaat ctcttcttat acaattgaag aacaaagagc
    19261 ggtccaaaca gaaatcctgt ttggaccgct cttttatttt aaacattttt cattaccaaa
    19321 atattcgagt cgcgtttctt atgcttcgat ctctgtaacg atacctgaac ccactgtccg
    19381 tccgccttca cgaattgaga atgtcgttcc gttttcaaca gcgattggat ggatcaagtc
    19441 aacatcaatc gtcacgttgt caccaggcat gaccatttct gtatcttctg gcaatgtgat
    19501 tgttcctgta acatcagttg tgcggaagta gaattgtgga cgatagttgt tgaagaatgg
    19561 agtatgacgt ccaccttctt cttttgtcaa cacgtacact tctgctttga attttgtatg
    19621 tggagtaatg gaacctggtt tagcaagcac ttgtccacgt tcgatatcgt cacgttggat
    19681 cccccgtaac aacacgccta cgttatcccc agcttccccg taatctaacg ttttacggaa
    19741 catttctaca cctgttacaa ctgctttttg tgtttcaggt ttgatcccta cgatctctac
    19801 ctcatcaccg acacgaacag caccacgatc aatacgacct gaggcaacag ttcctcgacc
    19861 tgtaattgag aagacgtctt ccactggcaa tagtaatggt ttatctgtat cacgttctgg
    19921 tgttgggata tattcatcta ctgtatccat caattccata atagcagctt cagcatctgg
    19981 atcgccttgc aatgctttta atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa
    20041 accatattcg ctcaataact cgcgaacttc catttctacc aaatcgatca attcttcatc
    20101 atcgactaaa tcaactttgt tcaagaaaac aatcaggtat tttacaccaa cttggcgtga
    20161 caataaaatg tgttcacgtg tttgaggcat cggaccgtca gttgcagaaa caactaagat
    20221 tgcaccatcc atttgtgctg cccctgtaat catgtttttc acatagtccg cgtgtcctgg
    20281 tgcatcaata tgcgcgtagt ggcgtttttc tgtttcatat tctacgtgtg cggtattgat
    20341 cgtaatccca cgttcacgtt cttctggagc tgcatcaata ctagcatagt cttgaggatt
    20401 tgctaggcct tttttgccta atactgtagt gatcgcagca gttaaagtag ttttcccatg
    20461 gtcaacgtgt ccaatcgtac caatgttaac gtgtggttta cttctgtcat aatgttcttt
    20521 tgccataatt tacaaacctc ctaaataata tagatcactg aaaaatttca gctctcacct
    20581 ttttattata gtcttttttt gactaaatca aaaagaatac gtccgactta ctttttgtaa
    20641 gattcgaact aagcttatta tacgtcaata ttggtcaaag tcaactaaga gcactcgctt
    20701 gtcttatacg cgaaaaaagt accttttata aataaaaaaa tgtgtttttt gagaaaaact
    20761 tcaagatagt ccttaacttt cttttgaagc aataagaaca aaatgaggaa aagtttaaaa
    20821 ttcagctgtt gccaacaact ttcaatagac gaaatcttct atttcgttaa taataaggaa
    20881 agaggtgaag tacatgaaaa aattaattgg cgtacttgtc gttttaggca tatttctcgg
    20941 cggaagtttt gcagcttatc attattttta cggcggcgaa gcttattata caaaaatcac
    21001 gacgaatggc gaaatgtcag ctacccaagc agataacgga gagaaattca cgacttatac
    21061 ctacaagcaa gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga
    21121 aagagaacat ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg
    21181 tgtattaagc tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat
    21241 cgataattag taaatggtct taaaatttta ttagataaaa agaggctggg acagaagcgt
    21301 ttaactccaa gaaataagaa ggagttgctt ctgcttccgc cgtttatacg tgtttagagc
    21361 gtgagacaaa agtgttcttt acttttgtct cacgctcttt ttttgttata ttcgtctttt
    21421 ccgatccttc tcttgacaca attcatttaa ctgatcactg gtacttgaac acctaatgtt
    21481 ctagtcattt ttataaacga ttcaatactt catccacatt tttgatcatc acggatatcg
    21541 taccatccgg attattcaca aattcaatca aatctgggtt gcgatataca tctactggta
    21601 cgatcagttc gatcccgttt gatagcttga atttttgttt tccaaatttt ttctcagaaa
    21661 tttcttgtac ttcttttaca ggaggtgctt ctgggacaaa tccttgctct tgtacttctt
    21721 ctttaaaagc catttttgcc gtgatgtttt ccttgaagac tttttccgca atcatctcat
    21781 gatccaaccg acctttttct tctatcgtat catagacagc ttctttcaaa tcagccatga
    21841 tatcaaattc ttctgtctca aatttttttc caagatgctt ggctactttt tggatcactt
    21901 ttacattttc ttctaaagaa ggtgcaggaa cactttcgat cactcttcca gaaaaataaa
    21961 gctctttttt gccagaaaac tcatattttt tttcaattaa ttcgtaggtc caatcagtca
    22021 aattcacagc gaatgcttca tctgcttttt gtgttttcgc ccctagaatt gctcgattca
    22081 aaatcaagcg gttgtcgatc cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac
    22141 gataattgac tttcaataaa gataagtaca tcactgtatc taattcatac aaaacaacaa
    22201 atacatctgc actaggtgct tcttcacttt cttggtagat atcgaaccac cgctgaacga
    22261 ttgcttcact tgcttccaca aaattttctt gagcgatttt aagtgattca acgatcatgc
    22321 tttcttctgc tagttgtcca gtttttgttt gagcagaaga gattttttgg atcttctttt
    22381 gaagaaaatc acgaatatat tcttttgtca gatccagctc tttttgtgaa aagactggag
    22441 aaccagtttc tctgtcaata atatgcagaa ttgctttttt taaatagata tccattttct
    22501 cattcccttc tgtcttagtt tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt
    22561 tcttttaaac aaaaatttca agagtagaaa caaaatggat tctttttttc gaaaaatgcg
    22621 ctacacttat agttgaacga ataaaaaagg agctgaaagt atgaattcat taacaagcac
    22681 ttatcgtcta ttaaatggtt acgaaatccc ggtagtagga ttcggcacat ggcaaacacc
    22741 tgatggggat gtcgctgttt cctctgttaa agaagcgctg gctgcaggtt atcgccatat
    22801 cgatactgcc caaggataca agaatgaaga aagtgtcggc caagcgatta aggaaagtgg
    22861 aattcctaga gaagagatat tcttgacaac aaaattatgg aatgcaaacc acagctatga
    22921 acttgtgatg tcttcttttg aagaatcatt gaagaagctc caaacagatt atttagatct
    22981 gtttttgatc cactggccaa accctgttgc ttttcgtgat aattgggaac aagcaaatgc
    23041 agatacatgg cgagcatttg aagaactgta tgaagcaggt aaaatcaaag caatcggtgt
    23101 gagcaatttc ttaccccacc atctagatac actagcaaaa acagcaaaaa ttatgccgat
    23161 ggtcaatcaa gttttccttg caccaggcga attacagcca actgtagttg agtatgcaaa
    23221 aaaacatgat atgatcctcg aagcatatag tccattaggc actggtaaaa tcttcgatgt
    23281 gcctgaaatg aaacaaatcg ctgaagcaca tgacaagacg attgcacaag ttgctttgcg
    23341 ttggtcgctg caacacgaat tccttcctct tcctaagtca gtgacaccaa gcagaatcaa
    23401 agaaaatact gaattatttg attttgaatt aacagaagaa gaaatgaaac aaatcgatca
    23461 gttagatggt gtagtcggca aagcaaaaga cccagatact acccaatttt aagtcgaaag
    23521 gtgattttat ggatccaatc gaacgcttga attctctctc ggaagaagtt acccagactt
    23581 ttcattccga ttttgtcttt ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa
    23641 actggacaca tgatcaaatc atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg
    23701 tcaaaccctg gcacgaacat gaagtcattt attcacctga actacctgtt tttgccttga
    23761 taccgaaaaa atagaaa
//
LOCUS       DAZUKD010000037        23517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000037 DAZUKD010000000
VERSION     DAZUKD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.038"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..2752
                     /gene="clpB"
                     /locus_tag="ACG8N9_001224"
     CDS             143..2752
                     /gene="clpB"
                     /locus_tag="ACG8N9_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4896838.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2903..3589)
                     /locus_tag="ACG8N9_001225"
     CDS             complement(2903..3589)
                     /locus_tag="ACG8N9_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896839.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3567..4061)
                     /locus_tag="ACG8N9_001226"
     CDS             complement(3567..4061)
                     /locus_tag="ACG8N9_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4896840.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4081..5301)
                     /locus_tag="ACG8N9_001227"
     CDS             complement(4081..5301)
                     /locus_tag="ACG8N9_001227"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896841.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5448..7283
                     /gene="lepA"
                     /locus_tag="ACG8N9_001228"
     CDS             5448..7283
                     /gene="lepA"
                     /locus_tag="ACG8N9_001228"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4896842.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7479..8390
                     /locus_tag="ACG8N9_001229"
     CDS             7479..8390
                     /locus_tag="ACG8N9_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896843.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9386..9637
                     /locus_tag="ACG8N9_001230"
     CDS             9386..9637
                     /locus_tag="ACG8N9_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896844.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9750..9992
                     /locus_tag="ACG8N9_001231"
                     /pseudo
     CDS             9750..9992
                     /locus_tag="ACG8N9_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10082..10321
                     /locus_tag="ACG8N9_001232"
     CDS             10082..10321
                     /locus_tag="ACG8N9_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896845.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10468..14751
                     /locus_tag="ACG8N9_001233"
     CDS             10468..14751
                     /locus_tag="ACG8N9_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS4896846.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14850..15215
                     /locus_tag="ACG8N9_001234"
     CDS             14850..15215
                     /locus_tag="ACG8N9_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896847.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15217..15669
                     /locus_tag="ACG8N9_001235"
     CDS             15217..15669
                     /locus_tag="ACG8N9_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896848.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16118..17593
                     /locus_tag="ACG8N9_001236"
     CDS             16118..17593
                     /locus_tag="ACG8N9_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS4896849.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17616..17927
                     /locus_tag="ACG8N9_001237"
     CDS             17616..17927
                     /locus_tag="ACG8N9_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896850.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18288..19544
                     /locus_tag="ACG8N9_001238"
     CDS             18288..19544
                     /locus_tag="ACG8N9_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4896851.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19541..20086
                     /locus_tag="ACG8N9_001239"
     CDS             19541..20086
                     /locus_tag="ACG8N9_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896852.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20087..20587
                     /locus_tag="ACG8N9_001240"
     CDS             20087..20587
                     /locus_tag="ACG8N9_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896853.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20946..21224
                     /locus_tag="ACG8N9_001241"
     CDS             20946..21224
                     /locus_tag="ACG8N9_001241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896854.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21244..21588
                     /locus_tag="ACG8N9_001242"
     CDS             21244..21588
                     /locus_tag="ACG8N9_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896855.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21607..22518
                     /locus_tag="ACG8N9_001243"
     CDS             21607..22518
                     /locus_tag="ACG8N9_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4896856.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22597..>23517
                     /locus_tag="ACG8N9_001244"
     CDS             22597..>23517
                     /locus_tag="ACG8N9_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4896857.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITK"
BASE COUNT         8133 a         3747 c         4944 g         6693 t
ORIGIN      
        1 tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ctatgtgtat attttgacca
       61 aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt gatcttacct
      121 ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc ttcaagaagc
      181 gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg acattgccca
      241 tgtatggaaa atctttttac aatctaatca ttttggtcga aatttctata cggatgccgg
      301 attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt atccagttgt
      361 ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc gattattgaa
      421 tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg aagtcgttat
      481 tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata aaaacggatt
      541 atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa gagtgacctc
      601 acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt tagtacagca
      661 agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc gtgatgtcat
      721 ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac ccggtgtagg
      781 gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg tgcctgaaaa
      841 tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg gagcaaaatt
      901 ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa aaagtgatgg
      961 acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa aaacagaagg
     1021 aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat tacatttgat
     1081 cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag cgttggaacg
     1141 tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca gtattctgcg
     1201 tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca acgcattagt
     1261 agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg acaaagcaat
     1321 cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta tgccaacgga
     1381 attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg cactgaaaaa
     1441 agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat tagctgaact
     1501 tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag aagtcaattc
     1561 tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag atgcagaaaa
     1621 taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc aattagaaaa
     1681 agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg ttcaagagtc
     1741 cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc ccgtaacgaa
     1801 acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc ataaacgagt
     1861 gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt cacgggctgg
     1921 attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa ctggtgtcgg
     1981 aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag atcatatggt
     2041 acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag tcggagctcc
     2101 tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca gacgaaatcc
     2161 ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct tcaatatctt
     2221 gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag tagatttcaa
     2281 aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg aaggcgtgac
     2341 tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat tacgtggaaa
     2401 cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac cattgagcct
     2461 agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac gtctggaaca
     2521 tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg aaaatgctta
     2581 tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag tagaaacacc
     2641 actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa cgattacttt
     2701 acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct aatcttccat
     2761 tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc ttttttcaaa
     2821 taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt gaagctgccc
     2881 gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta taactttcaa
     2941 agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag gcagcaccta
     3001 tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg acaaacagtg
     3061 tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg cataagaatt
     3121 cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat aagaggaaca
     3181 accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc aatccatagc
     3241 caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa aaagagatga
     3301 tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa aaccagtaaa
     3361 acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact tgtggcatga
     3421 aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga gaagtcgaat
     3481 atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca aaagcaagta
     3541 aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc taaaaatctt
     3601 tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa tttgatcaaa
     3661 cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg tagtgcagcg
     3721 atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc gatcactggg
     3781 atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat cgtcagccca
     3841 tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc aagagcaatc
     3901 attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg aggaagatag
     3961 atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt atataacaaa
     4021 tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc atccttttta
     4081 tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta gtgacttcag
     4141 tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac ggcgataatt
     4201 catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta taggttcatt
     4261 taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat tattccaagg
     4321 agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa acggaaaccg
     4381 ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga tctgctcttt
     4441 gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat ctccatgtac
     4501 ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc gagcttcttg
     4561 atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt agaacagaac
     4621 acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat cttcattttc
     4681 gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat ggttttgttg
     4741 ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc gctcaaccat
     4801 accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg aaagtttatc
     4861 agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat acaaataaga
     4921 gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat cttgcatcaa
     4981 tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca ttttataacg
     5041 tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca gtttattttc
     5101 gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac ggataaaagc
     5161 tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga acacatagga
     5221 aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt caagattttc
     5281 tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa cagtatagca
     5341 aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa ttactgcttt
     5401 tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg gatataaaga
     5461 aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat atcgaccatg
     5521 ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg tctcgcgaaa
     5581 tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc acgattaaat
     5641 taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc tttcacttga
     5701 ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt gctgcctgtg
     5761 agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca ttggccaatg
     5821 tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa atcgacctac
     5881 ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga atcgatgcta
     5941 gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt ctcgagcaga
     6001 tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa gctttgatct
     6061 ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc acggacggta
     6121 tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt gatgttacag
     6181 aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc ggtgatgtcg
     6241 gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat acagtaacgt
     6301 tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat ccgatggttt
     6361 attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa gctttagaaa
     6421 aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa gcattaggtt
     6481 ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa gaacgcttgg
     6541 aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac catgttaata
     6601 aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa cctgtaacga
     6661 ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat gattttgtcg
     6721 gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg gattatttag
     6781 atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc tttgactttt
     6841 tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa atgtctggtt
     6901 atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa gtcgatgcat
     6961 taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc gttgaaaaac
     7021 tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct attggacaaa
     7081 aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct aaatgttatg
     7141 gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg aagaagcgga
     7201 tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt ctgaaaatgg
     7261 atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa aacctattaa ataaaccttt
     7321 ttgagtagat gcaacactga ggattatctg tataaaagat tttaaactgt catttggtta
     7381 tcacggcgat actaaaatta acaaaattgg aatatttttc ttgaagattt ttagctgtca
     7441 gtatatgatg aaagcatctc ataggaggtg cttttttcat ggttgataat tcaagtggaa
     7501 gggttcttaa atctctaaga aaagagaaaa aactatctca aaaaaagtta gctgatttag
     7561 caggtatttc acaaagtact ttagttaaat atgaaaaagg gtcaagaaaa atccctaaag
     7621 acgtagataa tactctaagc aagattctga atatagaaac tctaataaaa gatgaggaag
     7681 ataagattga gattctcatc ggtaaactaa ttgcatacag agatatgaat aagttactta
     7741 acaaagagtt agctgataat ataggaatta gtgaagtatt actcagttat gtgttaaata
     7801 gaaagagaaa tccgtcaaaa gaaatgcaaa aaaaaatcga tatttttcta ttaagtaatg
     7861 aaaaagaaat tttgaaggag attaatcgtg atagtgagat atttagttta tcaaaagacg
     7921 ataaaattgt tatgggaaaa agaatacgag aagtccgaaa gaaccgggaa gagacgcttg
     7981 aaaaatttgg taaaaacttt accatctata caggcaaaaa tgttataagt agatgggaaa
     8041 aaggtattaa tattccagat attgaaaaac taatgaatat tgcctatctt ggtaaggtca
     8101 ctgttcctta ccttatgtac ggagaagatt ataagaatat acttccaaaa gatgaaagag
     8161 ttagcgattt taaaaagata aattctttta gtatgggatt aaggatgagg aaaattagga
     8221 aagattatta tctagaaagg gaggagtttg gaaagctttt ttctccttca atttcaaaat
     8281 ggtccataga taggtatgag aatggtagag atatacccaa tactaatcga attatccaat
     8341 atgcttatat aggaaatctt tctttagaat ttttgattta cgggatataa aaatagttta
     8401 cgtcgatact tattaagtta tcgacgtttt ttattataga aaaaatttat aaaaggaggt
     8461 ttcttgatat gataagaaac aaaaagggtt tgttgaaagt tgctttactg gcagcttttt
     8521 tctaagtaac tttttgacaa gctcaatggc aacacaagcg gaagaagtaa tacaagaact
     8581 aactgttatg aataacagct agataaaatg ggctatgtat gttatccaac aatttagtaa
     8641 tcactagaag cctagcgaaa gttcgcttga taagttatag atgagtattg atttgtagtg
     8701 tttccaaatc aaaagggtac agagccacat gaaatatgtt gtcctctgca gcgtcaggat
     8761 gggaatccat accatggaga ttcggagatc tgtgagggaa tacttacgtg gttaatatgt
     8821 cagttgtgtt ggacgtagta atggtcatag tgggtagatt gactgaattc ttatatgaga
     8881 aaatgaaacc aacaagaaga tagggttatt tatacagaat tggggatctt ttctggaaaa
     8941 gtaatcggat aatgcgagat tcgtacggtc ttttttaaat ttctctaaaa aaaacggcat
     9001 cgaatgggtt gggtgattga taagcaatga atcatagact gatatagaat aaaaaatgat
     9061 tctatttatc tttttacatc tatcgttttt tcaagtagcc gcaaattata tcagcattca
     9121 acaatttttg tatacaagtt ctagtaaaag gatctgaaga aaaagttggt tgttgtgaag
     9181 ataaaaaaag aatcaggttt tactgagttt ttcttcaaaa tttgtgttat taggttagga
     9241 caaagataag aagaagtatg aagatttaaa aaaggaatga ggaaaagata gtaatttccg
     9301 attattggag tttttttatt tataaagaag aagcgaataa gttgtgattc ctagatttcg
     9361 cgcgtgatta attaatgagc aaaaaatgcg gaaagtttta gtgtttaata cacgaggaca
     9421 aagtttctta ggtattgaag gcacacgtgt aacggttgat gggtggtgtg attgtttcga
     9481 ggttggaaaa catgttgaac ttatggcatt tactgagcta aaagataaga acaacgtgga
     9541 atttttcgag agtgatattg gttgggacga tcatgaagaa gtttatggaa aatttatttt
     9601 gcggaaggga aattcttata cgaatgggga agcataagta gcgatttgtt agagatcatt
     9661 gataggattg aaattgtcgg gaatatttat gagaatctag agttatttga ggtgatagaa
     9721 tgagtgatgg ttttgaacaa gtatcaataa ttaaaaatca agttcgggag attcttagaa
     9781 aaaaaggcta tttggtagat agttattttg aaggagatta cgaaacatgg gtaggtgtct
     9841 atgctcgtcc agagaataaa cctacctatt tagatcctac gacgtcagaa gatgcctatt
     9901 tacaaaatag gtatcgagta gatggtttta agcaagactt tgcggagtgg tttgaatggg
     9961 aaattgaaaa cggagaagta aaagaagaat aagagtactt gttgtatcaa aataagccag
    10021 tagaacaacc aaaccagtag ctagtaatga caataatact gaggaatcgg atcattatgg
    10081 tatgattgat atttctcttc cagaagatct taagccagat cctgtaacaa ttggcaatag
    10141 tgtcatgatt tttgattcat tagatgaagc tatgtcatat ggttggaaag aaattaagga
    10201 tgaaggtagt aattggtatg gcatgagatt tggtgatggt gaagttgcca aaaaagctgg
    10261 tattccaatt gggaaatgga cagttgattt ccacgaagca ggttcttttt ttgttctgta
    10321 attatagtgg gagggtaaaa tcattgatga aatgtgttca gataagagcg ccagataaaa
    10381 atagaagaga cagttcacgt cgttatcact tttggtaacg gcgttttgtt ttattatata
    10441 aaattctcag gaaaaggagg aattcttgtg tatgcaaaaa cgaaaatgaa ttggttgaaa
    10501 gcactcgcat tggtggcttt ttctcaattc tgtttcttgg tgggaactgc tcaagcacat
    10561 gcagaagaaa cgacggctat acaacctacg atggaagcca ttgcccaagt cgcttctgaa
    10621 gtgacagaag tccaaccagc agaagccggg gtcccagctg gtgaagccac tgttgaacag
    10681 gcccctgtga cagaagcgcc aacggtcaat tccaaaacga ctctcgatca gacgattggg
    10741 gaaacacaac acccagggaa tagtcctggt acttcacaac cagttgaaga aactgcctca
    10801 attacaaccc ctgcttcaga aacgtccact ctagaaggag aaaaagctgc cgaagcaacg
    10861 ccttctgtgc agccagagtc ttctgagaaa caagcggaag aaaagaaacg aatagaagaa
    10921 acagtggaac aaccagcggc tactgctgac aaagaaggaa aaaatgagga aaacgttgaa
    10981 aaaacaatct cttcaaccgc agctagtcaa aatcaaccaa cgaaaccaaa caaagaatct
    11041 acggctagta ttgaaaatca aaagaaaaaa gagcagaaca ttccaaaaac aggtacacct
    11101 acactgttag taacagtacc aaccaatcgt tcggttcgtg ttcgccgtgc aaccaatgca
    11161 gctgttttag gtggtcggaa aattgattgg ggcgaaaata ttggtgttac ttctagtccg
    11221 aacaatctaa acttcacttt aaattatatg gttcgttttg agattaatgg acaagtggcg
    11281 ttttgtattg agcctggtgt ctcagccgca gaaggtcaag tttatacagc tactgctttg
    11341 aaaaactatt tgaaaaattc aaatgtacgg aaaaaaacaa gcttgatctc ttatttagga
    11401 tatgtcacaa ataaagacaa atccgatgaa caatacattg cggcacaatt tatgatttgg
    11461 gaagctttgg gcacaaaaat cccaatcaaa aatatccgtg ttgattatga tcgtcgaaaa
    11521 gcagaaattc aacgtttgat taataacttt ggtgaaaaag caacttttaa taatcaaaca
    11581 catacggtaa aaattggcga aacgttgaaa gtaaagaata aaaatgattt tttgaaatat
    11641 gtgaaagaca ttcaaaaacc aaaaggtgtc aatgtatcta ttcacggcaa tgaagtatgg
    11701 attacagtca caaaagaggc accggataaa gcaaaaattc aattgaataa aattaaagtt
    11761 caaggtgcgc cattagccta tcgtcgtcca ggagctcaaa caattggtgt gttacatcca
    11821 acagaaccta atgcgtctgt tattaattta caaattctaa aacaaggaaa cattcgtgtg
    11881 tttaaagaag atgcggaaac tggtgtgaaa gcacaaggtg cagcttcttt agaaaatgcg
    11941 gtctatggtt tgtatcaagc agatgggaaa aagattaaag aaattacctt gaaaaaaatt
    12001 aacggcaaag tacaggcaga aattaaagat cttgatcctg gcaaatatgt tttaaaagaa
    12061 atcaaagcgc caaaaggtta tgtgctttct gatgaagcaa ttgaaattgt tgttgaacct
    12121 ggaaaaactg tcaatgcgac cgcaaaagat caagtgatca aaggaaccat tgatttagtg
    12181 aaagttgcta ataaagattt agtcgattca acgaatccag ataacaaacc aaaattagct
    12241 ggcattgaaa tcactttgac gtcaaaaaca actggcaaag tggtgaaaac cgttgtgaca
    12301 gacaatgatg gttatgctac ttttggtaaa aataccgttg tctttgatac gtacattctt
    12361 tctgaaacca aaggaaaaga agggtataaa ctatttgaac cacttgaagt cacaatttct
    12421 gaaaatgggc aaactttcca ttatgtttta gaagataaag taattgaaca acgtcttaaa
    12481 gtggtgaaag tggatcaaga aacaggaaaa gtgattccac ttgctaatac agaattcaag
    12541 atttttgata ctttagccaa tcgttatgtc acaatggaga ttccaaatga tacagaaaca
    12601 acggatatct ttaaaaccaa tgaaaaaggc tttttcctaa ctaacggtac acttacttat
    12661 ggaaaagatc gctatcgttt ggaagaaatc aaagcccccg taaattacgt tttgaatcaa
    12721 aacccattag tgttctcgat taccaatgat acagaaccga tcaaagtgat ccattttgcg
    12781 aaccgtcaag cacggaaaag tgtaaaatta ttcaagtatg aagaatggaa caagaagaaa
    12841 acgccgctcg caggtgtgaa ctttaccttg tataacggta aaaatcaagc aattggtgaa
    12901 tataccactg atcaaaacgg ggaaatttta gtgaaaggtc tagttgccga tgactattac
    12961 ttcttggaaa atgccccatt agaaagtttc caaccaaata cagataaaca agcattcacg
    13021 gttgcattca aacaaaatgc gccagatgat ggggaaacgt tagtggtttc tgtacgtaat
    13081 tacttgattc caccaaaact agaaacacaa gcgtcaaata aagccgatgg tgaaaaagtg
    13141 gttgatccat tagaaacgat cgtcattaaa gacagtgttt cttataccaa cttgtttgtc
    13201 ggaaaagaat acaccatttc aggagtattg atgaacaagg caacgaatgc gccaatttta
    13261 caagatggca aagaaatcac aagtaccttg gcctttattg cagacaaagt caatggcaca
    13321 aaagaccttg aatttgtggt taatgctagt gttctgaaag gtcaaacgat cgttgttttt
    13381 gaaaaacttt tccgtgatgg cattgaagtt gcagcacatg cggatatcaa cgatgaagcc
    13441 caaagtgtga aagtgaataa tccaaccgtt cataccacgg ctgtctttga agacggattg
    13501 acagtcgcag acccaacagg attgtttacc ttaaacgata ccgttgaaat taccgagatt
    13561 cttccaggaa aagcatactt ggtcaaaggg atcttaatgg ataaagcaac cggtgaaccc
    13621 ttgcttgtgc atggaaatac ggtagaaacg agtgttggct ttattgccaa agcagaaaat
    13681 gaaacggtga tcgtaccatt tacctttgat ttgacaggta taaatggtcg tgaaatcgtc
    13741 gtgtttgaaa gtctttttag aaatgatgaa gaactagcaa accataaaga catcaacgac
    13801 aaagggcaaa cggtacgagt aactaaccca agtgtacgta caacggctac acataaaccc
    13861 actggcttga aagaggttaa tccattaacg aaagtcacca ttacagacgc tgtgaaatac
    13921 cacgatttgg tcgttggaaa aacatacaca gtcaacggtg ttctaatgga caaagtaacg
    13981 gaaaaaccag tgctagtaga tggaaaagaa gtcacaagta ccttaacgtt tgtggcagaa
    14041 acaacggatg gtacagtcaa tctagacttt attctagacg cacgtgcgtt gcgaggcaaa
    14101 gaagtcgtgg tctttgaaga tctgtttaga gaaggcgtat tattggcttc tcacgcagat
    14161 attaccgaca atgaccaaac catcaaaatc agtaatccaa ccattacgac gaaagcgacg
    14221 aataaaaatg gcggaaaaga gatcttggcc ttgccaaatc aaactgtgct tgaatgggtc
    14281 aaagtagacg gtcttgcgat tggtcaaatc tatcgcttaa ttgttcaagg ctacttaaca
    14341 ccagacggca caccatttga aggcactcat gcggaaaaag tctttgcggc cgataaagaa
    14401 atgatggaat tcttatttga attcttagtg gacggtcgca atcttgcagg aaaaggcttg
    14461 agttttgcgg aatggctcca agcgaaaacg gaagatgaga aagaaacgtt tgaagaagtg
    14521 gcgaaacaca atgtagatct aacaaacaaa gatcaaacgg ttcaaattgt ggaagcgcca
    14581 aaaccaccgc aaccagtgaa accagaacaa cgggttaaac cagctactgt tgtaccaatg
    14641 ttgccaaaaa caggtgaaca aacaagtagt atacttatgg ttcttggatt agtagtatta
    14701 gtcattgctt taggtataac actttataaa gtacgtaaag ccaaagatta gtcatgagga
    14761 acaagcggtt aggattttta accgcttgtt tgattgtaat gtagaaatat ttagaattta
    14821 ttttggtttt taagaggagg aaaaacttta tggcagaaaa agcattgcgt atgaagaaag
    14881 agtttttagg tcaagtattt ttattgggtt cacgggaacc taatccacca aaggaaatta
    14941 atggtgtgtc tgggcgatat gttcaattac aagcagagtg ttcgcgagaa gaacagcctg
    15001 ctttttttgt ttatctcaca gctgaagagg ataaaagatt acgattacca gaaaaagagg
    15061 tgcgtatttc atttgaagaa gcttattttg tttttcgtaa agaacgagtg aatagtcgtg
    15121 aaacaaatga tatgggacaa acagagcgtc gaacaactgg ctatcaagat gttggtgtct
    15181 ttttaactgg tgtaaaaatt ggggaggttt tgtaagatgg gattaaaatt tccaaataat
    15241 tatgacatta cagaagcatt agatgaaact tgtttaggag ccgtacgctt tggcgcatta
    15301 gaagagtctg atcgtgtatt tgataatgaa acaggcgatt atacgggaga aattcgatca
    15361 cagcgagcac aattagtttt tggaaatcca gatgaaccaa agattgcacg acgagttaaa
    15421 tttccagcag atgtagattt gactggtttt aaacgacgtg atcctgtgaa attaattgat
    15481 ccagaatatc atgtggtata tattccagca caagatggca atcgtgaata ttcagattta
    15541 gtgatttatg cgaaaggatt agagaaagta gaagctccaa caaataatcc taatatgaaa
    15601 caatcacaag aaaaaggttt agatcaaaaa caaccaagtg ttgattctaa ggaaaaagag
    15661 aaaaaataag gtttttttga aagaatagga aggagtcttc ctattctttt tatctaagat
    15721 agaaggaagg ggagattttg aattcagata aaaccaatca aatactttga gagattgtgc
    15781 ttgttatcaa aatatccaac aattgattga gtattttgga gacaatccaa cgatttttgt
    15841 acaaacaaat gcatatattt gtgcggttat tgggcagtgt atgtttttac gtgatcttgt
    15901 tcgtttttca ttaccgtacc agtggaaaca agatcatcag gaagtgattt ggattcgtat
    15961 agtacgaata tgtaatatgt gtgcacaccc ttataaaaat cgattaaatc tttttcgatt
    16021 atgttttttt actaaatgtg atattcctgt attagttgct ttttgtcaaa agtttttagc
    16081 acaggaaaag taaaaacaga aaggaggaat gtgacaattg aaagctcatt ttttcaagta
    16141 tcgtggtcgg cgaattcgct atttcaatcg ttatttatgg ttttgctatt atggtttctt
    16201 tttgtttcca tttttgattc tgttaggttg gtttagttgg aaagtatttt atccacgttt
    16261 aagtacgttt tcgaattggc aaatgtatct tattcctgga ataagtattg tgttgtattt
    16321 gttgctggtt agtgggttaa ttttagggtt gatgcattgg tcgcgactaa aagaaggata
    16381 ttttgcctct gtttattggc gtcagttact tgttcgaatg ttaattgaca atcggtttgt
    16441 ttatacaaag aaacaagtta gtgagaaaca aacaagggaa aaaatgagat taccaaaagt
    16501 ttatttttat caaaagaaac aagaattagt ggtttctttt cctttagatg gtgggcgttt
    16561 tcacgaacga tttttaaaaa tgggtcattt gttatctgaa gtatttttgc gtgatttaat
    16621 tcgtgaagaa cgagaaaaag cccgaatcaa ttatgtattc ctttcagatt ctggtcggat
    16681 tcctattgat caatgtatag cagaaaatgg acgtattcat ttaatgaaaa ctttggattg
    16741 ggtatatgat caatcaccaa atatgttgat tgctggtgca atcggtggtg ggaaaaccta
    16801 tttattgtat tctttaattc aagcttgttt aagtgttggg acagtagata tttgcgatgg
    16861 aaaagcggca gatttagcag cattaggtga cgtcgatgta ttcaaaggac atgtgtttta
    16921 caaaataaat gaagaaatga ttatttgttt acgaaatgca ttaaaagaaa tgaatcaacg
    16981 gtatgcctat atgaaaacca tgaaagatcc taaatatgaa ccagggaaaa attatgctta
    17041 ttatgggtta ccaccgcatt ttatcttttt tgatgaatgg gcagcttttt atggttcgtt
    17101 ggatatggaa gcgagaaata gagtcgataa attgattcag ccattagtat taaagggtcg
    17161 tcaagcaggg atgtacatta ttttagcaat gcaacgacca gatgcagaat attttccaag
    17221 tggtgttcgg gataatttaa cattccgtat ttctgttggt cgattgtctc ccattggtta
    17281 tttaatgact tttggagaag attttaaaaa taagccattt tacaacaaag atcaacctgg
    17341 tcgtggatat gcttttccag gatttgatgt acctgtagag ttttttgcgc catttgttta
    17401 caaagattat cactttatca aagaatttga gaaatttact gaaatgattg tctatgattt
    17461 tacagtaaaa gaggatagtc aagatcaaac gatgggagat gataatcagt tgttacaaga
    17521 acaactagag cgccaaatgt atttagcaaa agatccaaaa ggaacaagtt tttctgatgc
    17581 gcatattttg taatcaacta aggaggtgaa gttaaatggc agagagttgt tattatagag
    17641 tagctacagc aatacgaatg attaatcctt ctctttcttc cagaacgttt tatgattggt
    17701 tgaatcgtat tgaacaagta actgattacc gctttttgcg caaagaacgt gtttttacag
    17761 gaaaagttat taatcaagta ttgctaacga aaaaagatat agaacgattg actcgattgt
    17821 atcattatcg agtagattta gaagaggatt taactttgtc aatttatcgc gttttttctc
    17881 ctgaaaaata tagtgaaata acaaaattag atcatttaat tttgtagtta ttatctatcg
    17941 ggagggctct agacgtagtc tggtagccgc actctgcctg gcggtgcgca ttaaggctgc
    18001 gaagcatggg cctataatgc gcactcgcta gcagcgtgtg cagctcacaa agaggtcgtg
    18061 cgcttgcgca cggcctcttt gtgtaaagcc ccccgatata taatatgggg ggcagaaata
    18121 acataacaca tagaaaaaag ttttataaac ccttttgtat caagtgatta tctaaggtca
    18181 gcagttgtac tacttttccc aaaaaacgag ggggactagt tgatatatcg gtgtttctaa
    18241 agggttaaaa aaaatcgatt ttgttatttt ataaaaggag cgagaaaatg acaccgattg
    18301 agctaaaaag ctacagaaag aagttaagtg caattcataa tataagtgga gataaaaagc
    18361 cgctatcaca agcaagattt gcgcaattga tgggctgctc aaaagattac attagaaaga
    18421 tggaacaagg gaaaagaaaa atcccagagg attttgaaca acgaatcaag gaacgctttc
    18481 gattgtatat gaaaagcgga atagatcttc gagcaatgat tgattatttg cgtctttcct
    18541 tttttgggac agactacaaa acagtgattc aacgtgtatt acgaatggat ctgacagaat
    18601 ttgaaacacg tccaacgggc ttgtatcttt ttgactgtct ttctgttcgt gggaatatct
    18661 gggtgttctg gcattcaaaa cgaactgtaa cagaaaatgt acttgtgcaa ttttcaggac
    18721 aaggttgccg ggaatatgag gaagtcatgc gtgaacaaca aacagattgg cgaacatggc
    18781 tatattccat ttggaaaaat caaggaaagt tggaagctgg ttatacacgt gtgcaatgta
    18841 gccgattcga tattgcgtta gatgaacttt ggggattgga aacggaagaa catttcgatc
    18901 tctttgaatt actagaaaaa caaaaagcag gtttattaga aatggacttt aagagattcc
    18961 agaatattgg cggttgtttt ttagatgatg gctattttcg aagtgaagga ttatctttgt
    19021 attttggatc gcgaaagagt cctctctttt ttaattttta tgagaaacgt tttgaaattg
    19081 cgaaccgaga aaaaatttca gaattagaag cattaacgaa atatggtatt tataatcgat
    19141 atgagttacg gttagccaat gagaaagcaa cacaagcagt agaagcattt attttaggtg
    19201 attcaccgaa acgtttagga gagattggcg ttggtttaat taatgcaaaa cttattgttt
    19261 gggatagact ggaaagtgat gcacggattg ttgataaacg atgggcaaaa ttatttcgtt
    19321 cgatggaaga attggatttt acagtaaagc cagaaaaatt ctcattagat cgttcagaac
    19381 gctggttttt gaaacaagta gctccgacat taaagttgtt acaagaagca gatcgtattt
    19441 taggaagaga ctttgtacaa gaagggattg ataatgccga actaaatgac gagcaacaaa
    19501 actatctaga atggatggga acgtcaggag ggaaaattgc atgaatgcat atacgacaac
    19561 agaatggctg cgcctgttgg atttaaaggc tgcaatcgaa gcattaaatg aaaagatggt
    19621 tgatttgtct tattttcgtt ttcgagttcc ttatattgaa caagcggtaa aagctggacg
    19681 ttatcaggaa aaagagaact ggcaagaaat tgcacgctta ttagaagttc gtaagggata
    19741 cgaacaagaa ctcgaagagt tggaatttag ccgaaggaaa ggaaggctag aattcattcg
    19801 cttttatcga ttttcattgc caatacctgc gattttagcg gttaaaaaag gctgcgataa
    19861 aatgaagata tatgaaaact gtgtggcagc acttagcagc gaaaaacctc ttatggaaga
    19921 aatttcattg gtgactgtca cttgggaaat gagtcgccag ccaacagaag aacagacata
    19981 tctttcttta gaagagattg agatagaatt agaggaaatt ggtcgctatg ccacttgttc
    20041 tacctattgt ggatcggtca ttagtattgc tggtgtgatt gtctaaatga aaaaaattat
    20101 gatttatgtt ggtgcgtatt ggtcacgtga tccaacagta ttagaaaatc cagaggcggt
    20161 acactattgc ttgcgccaat tattctatct gtataaagaa cgattagaat cgttgattcg
    20221 tcagttacca tatactgaca gacgtttaga tgaacttctt ctaaggtatc cagcaatgta
    20281 taaacgacga aaaaatagat tgttaccaga agagtatccc attgaaaaac atgaacttga
    20341 agggcgattt gtggcttatt tctatgatga tgttcgtatg cgcttagtag aacaacgaat
    20401 ggaaatagaa aatgatcgct actattttat caattattgt aagaagcgta agtatcaagt
    20461 aactgatgat ttttatgatt gtattttaca agatggggaa gtgattctat ccaaaattgc
    20521 tccaagtttt cgtgaacttg tgccgattga ttttttgaaa aaatgtcatt tgcggattct
    20581 tccgtaaaaa agagctataa aaggaaagaa aagtagaagc caaatcggtt tctgcttttt
    20641 tatttttttt aggaaggaga agatggtatg tcactggaat ttttaggaga gccaattgaa
    20701 aaggaagagt tcgtttcgca ttatatggat ttgtttattc aggtgataaa aaactttgtg
    20761 cgattgatga attttatcca cgcgaacaag actatctagc cgctgaacga ataaaaatca
    20821 gtttattatg ggaaaaattc acagatgaaa ataaccgacc gccagattat cgctatttat
    20881 cagattgttt aacgaataat gtgctttcta aagaatacct atttcacaaa cgtgagaaaa
    20941 atcaaatgtc ggttgaacat ttttgtgatt gttatattgc attattgcgt agtcaacagt
    21001 tattaacagt agaacgatat ggtgcagagt ttggttatgt catgcaagct gagcgtcata
    21061 gagctacaag atgctatgaa aaagaagatc aagtgattga tggctatgaa gaattatgta
    21121 ttcgaaacaa tcgctatttg caacaacgtt tatatgaaca attgatgatg gatttttcaa
    21181 atctctatag agggtatgaa tgttcgatga ttcaagaaag gtagaagaaa agggggaatt
    21241 tttatgtggc gattattatt tttggtggta tgtggtatgg cattgttcta tgtgtatcgt
    21301 gtctatctct atccacgaat ggaacgatgg acaagttggt atagagatcc tttaaaacaa
    21361 cgaaaaggat tgaagcggta tcgttatgaa ttacttgttg aattttcgga tgggcgacaa
    21421 gcacagtatt atttttttgc aaactatcca aaatacaaca ccagtgaact attgattcaa
    21481 ttattcaaaa ataatccata tgttgggtta gaagaaaacg gacgggagtt cttttatcgt
    21541 tcagataata ttagtgcggc gtctatgaac aaaacacgaa ttgcataaaa aattggagga
    21601 ataatcatga aaaaaacaac attagcagtc gtaacaacac taggaatttc attattttcg
    21661 tttcaattag ggagttcatt ggtttatgca gatgaaactg tagaaacacc taagtcagaa
    21721 attgtagaaa cattgccagg ggaaggatta ccagatgttg agacagagct aaatggagga
    21781 acaacggaaa aaccagcaga acctgaacaa ttgcagtcag agaaacctga aaaagagcca
    21841 gtgacaccag aaccagagtt tcctgaatca actttaccga ctgattcgat cgaagaagaa
    21901 ggtgtgcata gttcaacaac aacggattca agtatagtca caactccgga acaatcagga
    21961 acaaccagtt cttctgaaaa gaatactgat tcgtcaagta cggaacagcc aacgatgtcg
    22021 tctaccagtg aagaacaaaa agaagaaccg aaacaacctg ttgaaaaatc agaagcaatt
    22081 ccaaaagaaa ataaacctat tgttccagca aaagaagtca cagtatcggt aaccccaagc
    22141 ggtgaaatta cgacgaatac ttctcaagga atgagtgtac cgattgtcac tagtaatgta
    22201 gaggaattga cccatattcc aacaccaacc accccattaa aagtagcagg tgatcaaaca
    22261 attgttggtg taaaagatgg tattccatta atccaagata gtgaagggaa tttggtaaag
    22321 gatttgtcga ttcctgtaaa gaaacttcca agtggcaata ttgaaatcaa aacagcagat
    22381 ggaaaaacaa aagtcttacc aaagactggt gaagaaatac atgttgcact ttctgttcta
    22441 ggaggagtgt tgacaagtat tgctggattt gttggatata aaagaaagag aaaaggaata
    22501 gaaaatgagg gtaattgaat ggactatttt tcagaaaaac aagcttatcg ttgtcaatcg
    22561 tacgtactag taaaaacata ctagaaggag tagcttatga aaaaacgaca actacggcgg
    22621 aaagaaaaag tttatacaga gaaagtaccg aagcaaaaga aaatccattc aaaaaaagca
    22681 gtgaaatgga tttggtgttt ggttttattt ctttgttttt cagggggctt ggcatttttt
    22741 caatccatga aaacgaaggt tcaagtgtcg tttttgaaag atgaaatcca acaattacaa
    22801 aagaaagaag tacaaagtcg gtcagaatta acgtacacac ctgaaattga atcctttatg
    22861 aatcgttttg ttgctttgta tatgaataca ttcgataatc atgaacaaga acaacaacgt
    22921 caagaaacat taaaaaaaga gttttatgca caaaacttta ttcaagaacc aggaagtgtt
    22981 tcaggtgttc gaaaattaac tgctgcttcg tttattggat taaaagaaat tgaaaccatt
    23041 ccaactgcta gttataaagt aacttatgat attcgctcga tgaatccaga gagtaagaaa
    23101 gaaacaagtg tgacaatcac acaagtactc aatcttccct tttctatttc aaacaagggc
    23161 tgtcgtatta ttgcttatcc ttattttacg gcactaccaa agaatacaga aaaacaacga
    23221 atgattactt atgatgacac attatacgaa acggttgatg cgtcggaagc gaaagatgtt
    23281 gaaggatttg tggaagattt cttagataag tatgcgaaaa acaatgcaaa agatatgtca
    23341 tacatgatga aagaacctga aggactcaat caaacagcta aagttgatca agtagagatg
    23401 aaagtattgc gttataaaga aacgaaaaaa ccttttattg taaaagcaac ggtcgtcttt
    23461 cgagatggtg atacgtcact tttacaaaaa gaacaaatga cgattgtcat tacgaaa
//
LOCUS       DAZUKD010000038        23515 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000038 DAZUKD010000000
VERSION     DAZUKD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.039"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1570
                     /locus_tag="ACG8N9_001245"
     CDS             143..1570
                     /locus_tag="ACG8N9_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896858.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1662..2027)
                     /locus_tag="ACG8N9_001246"
     CDS             complement(1662..2027)
                     /locus_tag="ACG8N9_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4896859.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2024..2389)
                     /locus_tag="ACG8N9_001247"
     CDS             complement(2024..2389)
                     /locus_tag="ACG8N9_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4896860.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2581..2829)
                     /locus_tag="ACG8N9_001248"
     CDS             complement(2581..2829)
                     /locus_tag="ACG8N9_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896861.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2828..2956
                     /locus_tag="ACG8N9_001249"
     CDS             2828..2956
                     /locus_tag="ACG8N9_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4896862.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3013..4707
                     /locus_tag="ACG8N9_001250"
     CDS             3013..4707
                     /locus_tag="ACG8N9_001250"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4896863.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4774..5103
                     /locus_tag="ACG8N9_001251"
     CDS             4774..5103
                     /locus_tag="ACG8N9_001251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896864.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            5481..6614
                     /locus_tag="ACG8N9_001252"
     CDS             5481..6614
                     /locus_tag="ACG8N9_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4896865.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6645..6965
                     /locus_tag="ACG8N9_001253"
     CDS             6645..6965
                     /locus_tag="ACG8N9_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4896866.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6994..7458)
                     /locus_tag="ACG8N9_001254"
     CDS             complement(6994..7458)
                     /locus_tag="ACG8N9_001254"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896867.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7589..8842)
                     /locus_tag="ACG8N9_001255"
     CDS             complement(7589..8842)
                     /locus_tag="ACG8N9_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4896868.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8871..9188)
                     /locus_tag="ACG8N9_001256"
     CDS             complement(8871..9188)
                     /locus_tag="ACG8N9_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4896869.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9214..9534)
                     /locus_tag="ACG8N9_001257"
     CDS             complement(9214..9534)
                     /locus_tag="ACG8N9_001257"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4896870.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9998..11413)
                     /locus_tag="ACG8N9_001258"
     CDS             complement(9998..11413)
                     /locus_tag="ACG8N9_001258"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4896871.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11430..12746)
                     /locus_tag="ACG8N9_001259"
     CDS             complement(11430..12746)
                     /locus_tag="ACG8N9_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4896872.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12896..13594)
                     /locus_tag="ACG8N9_001260"
     CDS             complement(12896..13594)
                     /locus_tag="ACG8N9_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4896873.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13883..14876)
                     /locus_tag="ACG8N9_001261"
                     /pseudo
     CDS             complement(13883..14876)
                     /locus_tag="ACG8N9_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14957..15790)
                     /gene="msrA"
                     /locus_tag="ACG8N9_001262"
     CDS             complement(14957..15790)
                     /gene="msrA"
                     /locus_tag="ACG8N9_001262"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4896874.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(16024..16767)
                     /gene="truA"
                     /locus_tag="ACG8N9_001263"
     CDS             complement(16024..16767)
                     /gene="truA"
                     /locus_tag="ACG8N9_001263"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4896875.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16958..17593)
                     /locus_tag="ACG8N9_001264"
     CDS             complement(16958..17593)
                     /locus_tag="ACG8N9_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4896876.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17740..18960)
                     /gene="sstT"
                     /locus_tag="ACG8N9_001265"
     CDS             complement(17740..18960)
                     /gene="sstT"
                     /locus_tag="ACG8N9_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4896877.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(19302..19979)
                     /locus_tag="ACG8N9_001266"
     CDS             complement(19302..19979)
                     /locus_tag="ACG8N9_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4896878.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20230..20772)
                     /locus_tag="ACG8N9_001267"
     CDS             complement(20230..20772)
                     /locus_tag="ACG8N9_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4896879.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20777..21004)
                     /locus_tag="ACG8N9_001268"
     CDS             complement(20777..21004)
                     /locus_tag="ACG8N9_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4896880.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(21083..22963)
                     /locus_tag="ACG8N9_001269"
     CDS             complement(21083..22963)
                     /locus_tag="ACG8N9_001269"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4896881.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23315..>23515
                     /locus_tag="ACG8N9_001270"
     CDS             23315..>23515
                     /locus_tag="ACG8N9_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4896882.1"
                     /translation="MDFIQELECTFHKKFTHSILLKLLIHTACLIERTLINGHELKII
                     SEDDTRPSHETIFHVKKAFKNIE"
BASE COUNT         7300 a         4829 c         3967 g         7419 t
ORIGIN      
        1 tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat gctttttcag
       61 aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa
      121 aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca
      181 gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa
      241 tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa aaaaagaatt
      301 tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga
      361 acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac
      421 ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt
      481 catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg
      541 cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct
      601 tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt
      661 tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa
      721 agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc agcaagtgtg
      781 tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta
      841 tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc
      901 ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc
      961 ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact
     1021 agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac cgttcgaacg
     1081 tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa
     1141 attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc
     1201 cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt
     1261 tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt
     1321 cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt
     1381 cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca
     1441 gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata
     1501 tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca
     1561 aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc
     1621 atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt ttggacatct
     1681 acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc
     1741 gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa
     1801 acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc
     1861 tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg
     1921 attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca
     1981 agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc
     2041 ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa
     2101 tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga
     2161 tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc
     2221 ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa
     2281 aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa caaatattta
     2341 aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct
     2401 actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat
     2461 ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccatttt
     2521 tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc
     2581 ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt
     2641 tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt
     2701 tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat
     2761 catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc
     2821 gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg
     2881 cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga
     2941 attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt
     3001 ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg
     3061 gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat
     3121 tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat
     3181 tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg
     3241 cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact
     3301 gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt
     3361 gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc
     3421 aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa
     3481 aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca
     3541 aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa
     3601 ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc
     3661 gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg
     3721 aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa
     3781 acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac actgactcaa
     3841 gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt
     3901 acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt
     3961 gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac
     4021 aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac
     4081 catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat
     4141 tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatc
     4201 gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt
     4261 tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat
     4321 ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg
     4381 aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat
     4441 atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt
     4501 cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg
     4561 atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt
     4621 attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct
     4681 cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa
     4741 gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta
     4801 ccgcagatgc aagcttttga acaccgttta ccaagaggtt gtgaaaaagc tgccctgcat
     4861 caagtaataa gaacacccaa gctaaaactg cttcttatgt ttttgacttg gttggactta
     4921 ttaccgcaga tgcaagcttt tgaacaccat ttaccaagag gttatgaaaa agctgccctg
     4981 catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac
     5041 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt
     5101 tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt
     5161 gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc
     5221 tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg
     5281 gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac
     5341 acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa
     5401 ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga
     5461 atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa
     5521 tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag
     5581 caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa
     5641 aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc
     5701 aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc
     5761 atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg
     5821 gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc
     5881 taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg
     5941 aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag
     6001 gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc
     6061 aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc
     6121 gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac
     6181 aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg
     6241 taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa
     6301 gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc
     6361 aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc
     6421 gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg
     6481 caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat
     6541 cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg
     6601 ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac
     6661 gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct
     6721 tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg
     6781 ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca
     6841 cctattttta tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa
     6901 ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg
     6961 catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag
     7021 acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc
     7081 ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc
     7141 ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac
     7201 aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac
     7261 gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact
     7321 ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca
     7381 ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat
     7441 ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat
     7501 acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aacggaaaac
     7561 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
     7621 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
     7681 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
     7741 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
     7801 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
     7861 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
     7921 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
     7981 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
     8041 cgataaacgg tgcgctatgc ccagctgcga ttgcattaac gtttgcttca atggctggcg
     8101 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
     8161 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
     8221 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
     8281 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
     8341 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
     8401 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
     8461 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
     8521 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
     8581 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
     8641 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
     8701 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
     8761 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
     8821 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
     8881 aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat agatgtcatc
     8941 agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
     9001 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
     9061 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
     9121 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
     9181 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
     9241 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta catctaaagg
     9301 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
     9361 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
     9421 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
     9481 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
     9541 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
     9601 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
     9661 agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
     9721 aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt attcaaaagc
     9781 ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt tttactttgc
     9841 tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac ggtgttcaat
     9901 cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta tttcaaataa
     9961 ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt tcgtcaggca
    10021 aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt ttcaatgttc
    10081 tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta aatgcattca
    10141 ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt gcgccttctt
    10201 ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg taatcatctt
    10261 gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata ccgttttctg
    10321 ttatcagcca agggatatta tgataatttt cttttagata catggctata tcatacatga
    10381 ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt aaatcaaaga
    10441 cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa cgaggggcgc
    10501 gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga tgctgaatcg
    10561 tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac aactccagga
    10621 actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta taaaacaaat
    10681 catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag accatttcag
    10741 gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc tctactgctt
    10801 tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg ttttgttcat
    10861 gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt tcgttgaatg
    10921 taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca tacttagcat
    10981 aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga aaagctggga
    11041 cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa gagtcgatca
    11101 tttcacttat atgctgagca tattcttcat ctactataca gttctcataa tcagtaaaaa
    11161 aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc atcagatcca
    11221 catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac ccattatgcc
    11281 aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct tctttttttc
    11341 ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa ttttttggaa
    11401 tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac acgatttgct
    11461 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    11521 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    11581 gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata accaatcgtt
    11641 ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    11701 ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga taacttggtg
    11761 atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    11821 actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    11881 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    11941 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    12001 ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa tgggcgttgt
    12061 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    12121 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    12181 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    12241 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtatct
    12301 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    12361 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    12421 tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata gatggtcaag
    12481 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    12541 aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    12601 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    12661 atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc taatttatcc
    12721 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    12781 tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct tttttaccaa
    12841 atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    12901 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    12961 tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg atctctgtat
    13021 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    13081 ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta gcttctgcaa
    13141 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    13201 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    13261 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    13321 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    13381 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    13441 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    13501 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    13561 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    13621 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tatacaaaat
    13681 tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    13741 taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat atgatgttca
    13801 ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta atatatggat
    13861 aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat tcttcttctg
    13921 gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt aaccgacttg
    13981 ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg gccatttgcg
    14041 ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt tccagagttg
    14101 atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta gaatcttcag
    14161 gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg aacgtgattg
    14221 cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt gcacgagaaa
    14281 acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt aaaatttcag
    14341 tcattttttt gtatcttctc cattatagtt ggatatacca atatataatg cttttccttg
    14401 tcttacaagt tgcatcaacg cttctgctgt ttcttccaaa ggtgtatcag ggtctgggcg
    14461 atgatgatag aagatgtcaa cataatctag ccccattctt tggaaacttt ggtcacaact
    14521 tgatatgatg gatttacgag agccccattc cccatatggc ccttcccaca tatgataacc
    14581 tgctttgcta gaaataatca gttcatcacg gtaggcttga aaatcttctc taaaaatacg
    14641 cccaaagttt ttctctgcag ctccagcagg gcctccataa ttattcgcaa ggtcgaaatg
    14701 ggtaattccc atgtcgaatg cgcctcttaa aatctctctt tgattatgga taggatcata
    14761 atcaccaaaa ttttgccata atccgagagg agagataaaa tcggtaattt taaacctgaa
    14821 tgaccggatt tacgatattc cattttttca taacgtgcac tatctgcaat atacaatttt
    14881 tctcctcctc atcaaatctt tttaaaacgc tttcttgttt tctattttta gtttactaca
    14941 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
    15001 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta tagcgtttcg
    15061 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    15121 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    15181 gttccgctaa ttctttttgt cgttcgtctt gataaaaaat cactggacga tattgcttcc
    15241 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    15301 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    15361 ctgtgtaacc accacttact tgatcataat tcggtttttc aacatgtccg cccgtgtatc
    15421 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    15481 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    15541 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    15601 caggcgtcag attcattcgg atagctaatg gacgtagtgt tacttctagt tttgataact
    15661 gttctttaac ggatatattt tctcttgtgt cattctttgt ctgaatcaat acttttcgtt
    15721 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    15781 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    15841 tatcataaag tgaggaaata atcgattgaa tcgtatttat tcaaagaaga taagcagctt
    15901 cagatgatat aagatgatct ttatcttaaa aagcatactc tatggtttgg tttaactaaa
    15961 aaacgacctc tgaaaaaagg gatgttctcc aattctcaga agtcgagtaa agatgattct
    16021 tttttatgag cgataataaa cttcatccaa aaacagacct tgtgcaggga cggtttctcc
    16081 tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt gtttctatcg gcatggcacc
    16141 tgtgccgatt tctagtaatg tccccatgat aatccgcacc attttatata agaatccgtc
    16201 tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg atagataatt catcaatcgt
    16261 acgcactgtg gatttttttg attttttcaa tgcagaaaaa ccaatgaaat catgtgtacc
    16321 cactaatttt tcgcaagctg cattcagctt gtccatatcc aactcttgag ggaaataaaa
    16381 gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc caaacataat agctgtattt
    16441 tttgcccacc gcattatatc ttgaatggaa gcgctcaggc atttcttcga ttcttttgat
    16501 gacaatatcc cgcggaaggt aacgattcat aaagtcgatc atttctgcca gtccaatttc
    16561 tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg gtgccagcat ctgttcttcc
    16621 tgatccaata atttctatct cttcatttgt catctgatgc aatacttttt cgatttttcc
    16681 ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct gaatatcttt ttccgtcata
    16741 ttcgattgtc aacttgatgt ttctcatatt ttagtggctc ctctgtcttc catttggtct
    16801 tctctctacc tttttaagat tgtagcacaa aatatagctc cttgcttctc taagacagaa
    16861 gctatacaag aaaactgtgg aaatatatat ttcagaaaaa caaaaaacag ccaccggatg
    16921 aagtatccgc tgactgttca atttgacggc tcaagtttta ctgtttgaga tgcaagatac
    16981 tcaaccaggc aatgggcaca taattaaaga aaaaactgat tctttggcaa aaacctattt
    17041 tattcagtaa tggccaagaa tttatagaag gaattcttgc cagtccataa atcgccgcaa
    17101 aaagcaaact gatcacagac agtccaaggt ataagtggct tacgtccatt tgcccatttt
    17161 ttcgatagat caagaaaaga agaactggaa acaataaaaa accactgtaa cctaaacctg
    17221 atccgatatt atggacccat gtcgagacat tccacgaaga ttcatgggta ttgatactga
    17281 acaaacctgt aaagatacaa tcacctatac catagaacgc gatggctgct gtaactaggg
    17341 tagctaacac acgagatgta cttacaaatg cagcatacaa tgcaggcaat gacaggatga
    17401 aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt tcttacagga ctggaaacct
    17461 ctccaaatac actgatcacg ctcttcattt gatctaattc aggataatac aatcctaata
    17521 cgtacggagt caaaaaatca ctcagcacgc ctaataataa aaagaaaaaa ccaaatttac
    17581 gtaaaaaatc catgatacgc ctccaataac aattacctat gcttttaatt gttattataa
    17641 acgactttat cttgatttaa cagaaacctc tatttacacg aacatctata taactcatta
    17701 aaaactcgaa tcattttaaa aatactaaat atctaacgtt caacgttcac ttaatccatc
    17761 taaacaatct ttcaatttga ttttttcccc acttctttta cgcgcgccta gctctcctat
    17821 tgcagtaaac aacagatcac ttgacgaatt taaggcagtt tccattgaat cctggataac
    17881 accgataata aatccaatac caacgatttg catcgcgaca tcattagaga taccaaacaa
    17941 actacatgct aatggaatca ataacagtga accgcccgca acacctgaag ctccacaagc
    18001 agctaaagca gacaaaatac ataaaaggac tttcaaaaca aatggtgctt caatccctaa
    18061 agaatgtaca gcagtcaacg tcatcaagga aatggtaatc gcggcaccgc ccatattgat
    18121 cgtagcacct aacgggatcg atatggaata agactctttt gtcaatttca atctttcagc
    18181 caacatcatg tttatcggga tatttgcggc agaacttcgg gtaaagaaag cgggaagtgc
    18241 gctttctttc aaacaaaaga aaatcaatgg atagggattt tctttaataa acaaaaacgt
    18301 gaggaatggg tacacaacta atgcgacaaa cagcatcgta ccgatcaaaa caagtaaaag
    18361 ctgcagatac tctgccaatc cagcgattcc agtcgtagca attgattgat aaaccaatcc
    18421 taaaatccct atcggagcaa atgcaataat cacttgtacg acttgtgaca acgctataga
    18481 tatctgatcc accaactctt ttgtcggttc tgaacgtgct cgaaaagcta gtcccaacaa
    18541 tacagcccag aataaaacgc caaggtaatt cgcatcaata agagattgaa caggattttg
    18601 tgtgacattc gtcaacatag tagataaaac actttccaaa ctttgcggtg ctttttcaga
    18661 tactgtttct tgtaaagtaa taggaacagt aaaaattgaa cttacaatga ctgctacaag
    18721 tgcagaaagc aatgttccca ctaaatataa gatcaaaata ggcttcacga atgtcttatt
    18781 cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa aaaaccagaa taggtgcgat
    18841 tgccttcaag caactgacaa acaacgtccc caatattgaa ataaatgtcc acgtggggaa
    18901 aaagatacca aacaatattc cgactaaaat accaacaaca atacgttgaa ctaacgacat
    18961 atccaaaaac ttctctaaca aatgaccacc ctttcacaat tttaacaatc attgtatcat
    19021 tttatctaaa gaaactaaag gtgcaatttt ctaaaaattc taataataca attggcaatt
    19081 catgctcttg caacattcaa ttttagtatt ggagggttta ttcaatctct aaagaagtcg
    19141 agagaaaact atttatcgtt attattatag ttatccaagt aaaacgtaac taagatgaag
    19201 gacgaaacta gaaatttcat ccttcatctt aattcttaga catagcaatc aattaatatt
    19261 ttccactgag tctcttcttt ctctcactct cactggttta cttacccata aacgatttga
    19321 ttaaagccat ttttcaattc attattcgct gtctcgttaa agtaaagata attatcggga
    19381 ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa cccgaataga
    19441 tagacgtgct ttttcagata tggttctgtc tctgcatcgg aatagacggc tgtgatttga
    19501 tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac tccaccaata
    19561 gccatcttcc actccccgtt caaaataact tgagggactt taagaccata taagtccaca
    19621 ctcatttgat tcgtatagct tttgtattgt tgtcccattg tttggcccca tgtagccata
    19681 aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcgac ttgttcgctg
    19741 ctttctgaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt tgtttcagtc
    19801 gttttggaag actctgacga agtagaggta tctgctgttt ccgtacttgc tgcagttggc
    19861 ttgctctctg tgctagttgt tgtagtagtt gcaattgttg ctttcgctgt ttcttttgtt
    19921 gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc tagaccaatt
    19981 gttactaaaa tccttttttt aaattaaaaa actcctttcg attactccca cttctttatt
    20041 ctatcaaata agcaatgtga ttttattgat tttttgtatt ttctcatcca tcttttaaac
    20101 cttttaaaaa ctaagaagca cccgctgatg ttgtctgtaa tacatcagcg ggtgcttctt
    20161 ttattttaat cttcgcgatt ttccaattta cctcacgctt tatatttgta tttgaagaaa
    20221 catagacttt tagtcatcat cgtcttcctc atccaaatct tgtcggacag tttttccttg
    20281 gagtctttgt acttggtaga tgatttcttt attgaaactt aaaagttcaa caagcaattg
    20341 tttcaagcca tatttttctt ctttttcagc taatttgatt gcccgtgtga taaagacatt
    20401 ttggaaatca aaatcttttg caaagttttc caacattgtt gaggctggta aatatttgtt
    20461 ttgaccatgt tcgtaaattt ttccataacg catgacttct tctaaagtag acggaacgat
    20521 ttcattttca atcactaact catgagagat tttattgaac catctcgctt cttgatcagc
    20581 atattctgaa aaaagggtac ttaattcttg cgaagatgta cccaaaacat acaattgtgc
    20641 ttggatcaat ttcatttctt ctaatttaaa atttgccaat acatgcccca ccatggcgcc
    20701 agcagttggt ttatgatgat cgatatctgc ttgttttgtt tcttgagcaa atttttcttc
    20761 cactatattc actgatttac gcctctttta cttttgttga ttggacttca taacctaatt
    20821 tttccacaac tgcagctaat tgttcagggt ggacttcatc tggatcaaag tttgctttga
    20881 ttttgctcgc attaaacaaa actttcgtat tgcttacccc aggctgttga gaaaccgcat
    20941 gttctatttt ttgcatacag cttggacaag ctagtgtatc taattgcata atcatttttt
    21001 tcatcgtagt tcttccttcc tttttcttga tagtcttatt ctagattaca gcttccataa
    21061 aaaaattgat tcttatcaag ttttacgatt tttcttcttt gctttcgtgt ccaaaataca
    21121 ataaacgcat cgcgttaaag ataacgatca agatactagc ttcgtgaacg aacatcccac
    21181 tagccatgta aataaacccg gcaaataagc cgatgagtaa aaataagacg acaccaatgg
    21241 caatcactac attttcacgg gtattcaaaa cagttttctt agccaaacgg taagcatgaa
    21301 ccaacgatcc aaaactagat tgcatcaaga caacgtcact cgtttcgata gcgacatccg
    21361 ttcctgatcc catagcaatc ccgatatcag ccgcagccaa agagggagaa tcattgattc
    21421 catctccaat gaaagcaacg tgcaatcctt gttcttggta ttttttcaca aattgaacct
    21481 tttcatctgg caataattca gcatggactt catcaatgcc taacatgtca gctacataat
    21541 cagcagtcat ctgattatca cctgtcagca tcactaaatg ttttgctcct gcttgtttca
    21601 acatagctaa ttgttcagcg acttctggtc gaatcacatc tgacacacca atgatttgag
    21661 tgattttttt gtcgattgct acaataatga ctgagctgcc tctttgttgt aattgatgta
    21721 aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc ggcaatcaat ttggtgttgc
    21781 ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc ttgtcccttg atcgtctgat
    21841 tatctttgac tgctaattgt tgaaaatcaa tgtctttctc ctgagcataa tggatcacgg
    21901 cgcgccccag aggatgatcg gataacgttt ctaatcgagc cactaaagct aagctgtccc
    21961 cttcatcctc tgtatagttc ttgatagtgg atacagcagt agttcctttg gtcaatgtac
    22021 cggttttatc aaagacaaaa gtatcgattt tggaaaaagt atccattgct tcaccgcctt
    22081 tgactaagat gccccgtttc gctccattac cgattcctgc tacattcgaa acaggcgcac
    22141 caatgactaa agcgccagga caaccaagaa ccaaaacagt gatggctaaa cgaaaatcac
    22201 gactgatcag accaacaagc aacgcaataa gtaaaacggt tggagtataa tactgagaaa
    22261 attgatcaat gaatttttcc gcatgtgatt tggtatcttg tgcttcttcg actaattcta
    22321 taattttggc aaaggtcgta tcatctccga ccttagttgc ttcgatcgtc acgtagccat
    22381 ctgctaaaat cgttccagaa aatacgttat ctccactgat tttgacgact tgtcgtgatt
    22441 cacccgtcac tgcggcttca tccgcatacc ctttcccgtc aatgactcgt ccgtccacag
    22501 gaatagctcc accagttttt actaagacga tatcgccttc ttccacgtca tcgatttcca
    22561 cttcttcggt cgttccatct tcttggacaa cgctggcagt cgatggggcc atctcggtca
    22621 aagccttgat tgattcccgc gttttggtca atgttttttg ttccagataa ctaccaaaca
    22681 aaaacaagaa tgtaacgata gctgactcct tgtattctcc gatcataaag gcaccaagca
    22741 cagcaatagt aacgagtaaa tcaattgaga taactttgac ttttactgcc tgatacgcat
    22801 ggattataat aggtatgacc gcaataatag aagctatggc tagagcaatg ttatagaaca
    22861 agacaaatcc agtaaatttc ccgataaaac caaaaataat caacacagct gataaggcgg
    22921 tgatatgatt tcgatgctct tggatccact tttgtaattt catctttctc cctcttttcc
    22981 ttttcattct tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc
    23041 aagtttattc aaaaaggcgg ttttcttttg gtatactgaa ttcctacagt cattaaacga
    23101 tcatatagaa aaaggaagcg ccgaaacgct tccttaggta gttctatttt tttattcaaa
    23161 gataagatgc tttgtcatct tacgactgaa gaacaacaat caaaaaaggg cagcagccac
    23221 tcattcacaa gaaatatcgt tttttgatat gggagttgaa gcagtggaca aatatctcta
    23281 cttattagct gggaataaga tccaaaaaag cttaatggat tttatccaag aattggaatg
    23341 tactttccac aaaaaattca ctcattccat tcttttaaaa cttttgattc atacagcgtg
    23401 tctaattgag cgtacattga taaacggtca tgaacttaaa attatatcag aagatgacac
    23461 aagaccttct catgaaacaa tttttcatgt aaaaaaggcc ttcaaaaata ttgaa
//
LOCUS       DAZUKD010000039        23408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000039 DAZUKD010000000
VERSION     DAZUKD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.040"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..1454)
                     /locus_tag="ACG8N9_001271"
     CDS             complement(162..1454)
                     /locus_tag="ACG8N9_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4896883.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1451..2074)
                     /locus_tag="ACG8N9_001272"
     CDS             complement(1451..2074)
                     /locus_tag="ACG8N9_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896884.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2077..2913)
                     /locus_tag="ACG8N9_001273"
     CDS             complement(2077..2913)
                     /locus_tag="ACG8N9_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896885.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2930..3817)
                     /locus_tag="ACG8N9_001274"
     CDS             complement(2930..3817)
                     /locus_tag="ACG8N9_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896886.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3833..5116)
                     /locus_tag="ACG8N9_001275"
     CDS             complement(3833..5116)
                     /locus_tag="ACG8N9_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4896887.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5799..6482)
                     /locus_tag="ACG8N9_001276"
     CDS             complement(5799..6482)
                     /locus_tag="ACG8N9_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4896888.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6500..7117)
                     /locus_tag="ACG8N9_001277"
     CDS             complement(6500..7117)
                     /locus_tag="ACG8N9_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4896889.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7127..7951)
                     /locus_tag="ACG8N9_001278"
     CDS             complement(7127..7951)
                     /locus_tag="ACG8N9_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4896890.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7944..8504)
                     /locus_tag="ACG8N9_001279"
     CDS             complement(7944..8504)
                     /locus_tag="ACG8N9_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896891.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8678..9634
                     /locus_tag="ACG8N9_001280"
     CDS             8678..9634
                     /locus_tag="ACG8N9_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4896892.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9939..10895)
                     /locus_tag="ACG8N9_001281"
     CDS             complement(9939..10895)
                     /locus_tag="ACG8N9_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4896893.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            10999..11601
                     /locus_tag="ACG8N9_001282"
     CDS             10999..11601
                     /locus_tag="ACG8N9_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4896894.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11689..13188)
                     /locus_tag="ACG8N9_001283"
     CDS             complement(11689..13188)
                     /locus_tag="ACG8N9_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4896895.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13204..13728)
                     /locus_tag="ACG8N9_001284"
     CDS             complement(13204..13728)
                     /locus_tag="ACG8N9_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4896896.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14026..14775
                     /locus_tag="ACG8N9_001285"
     CDS             14026..14775
                     /locus_tag="ACG8N9_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4896897.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14766..15551
                     /locus_tag="ACG8N9_001286"
     CDS             14766..15551
                     /locus_tag="ACG8N9_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4896898.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15520..16236
                     /locus_tag="ACG8N9_001287"
     CDS             15520..16236
                     /locus_tag="ACG8N9_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4896899.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16338..17357)
                     /locus_tag="ACG8N9_001288"
     CDS             complement(16338..17357)
                     /locus_tag="ACG8N9_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4896900.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17376..18377)
                     /locus_tag="ACG8N9_001289"
     CDS             complement(17376..18377)
                     /locus_tag="ACG8N9_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4896901.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            complement(18655..19803)
                     /gene="nagA"
                     /locus_tag="ACG8N9_001290"
     CDS             complement(18655..19803)
                     /gene="nagA"
                     /locus_tag="ACG8N9_001290"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4896902.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(19879..20715)
                     /locus_tag="ACG8N9_001291"
     CDS             complement(19879..20715)
                     /locus_tag="ACG8N9_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896903.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(20899..22116)
                     /locus_tag="ACG8N9_001292"
     CDS             complement(20899..22116)
                     /locus_tag="ACG8N9_001292"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4896904.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(22265..22426)
                     /locus_tag="ACG8N9_001293"
     CDS             complement(22265..22426)
                     /locus_tag="ACG8N9_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896905.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            22499..22582
                     /locus_tag="ACG8N9_001294"
     tRNA            22499..22582
                     /locus_tag="ACG8N9_001294"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22533..22535,aa:Leu,seq:gag)
     gene            complement(22822..>23408)
                     /locus_tag="ACG8N9_001295"
     CDS             complement(22822..>23408)
                     /locus_tag="ACG8N9_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896906.1"
                     /translation="LDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFG
                     NTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIV
                     GIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAV
                     KELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         7042 a         4918 c         4033 g         7415 t
ORIGIN      
        1 tttattttct cctatctaaa aaagcagccc acataaaaag aaggcatgac aaaaagcatg
       61 tcatagcccc tttttccgaa taaatagtgt tcaaaagaca gcctatcggc aataaggcaa
      121 gattttataa actacgggaa gtaatttccg taaatctatt cttattcttt taagctgaca
      181 agctcgtcaa tcactactgc ttttcctgaa gcaatagaat gatttgctgc gattcctgtc
      241 aaaatcgaca aaataccatc tacgtgagtt gctgcccgat tccacggatc ttcttctttt
      301 tcttcaccaa atagatcatc taataaacgc ggatctcctc cgccatgtcc gcccgcttct
      361 tgttcgacga ttacctcata tggctcaccg aacatcggat agacatggat ggactgaaga
      421 acggctgctc cttcatcctt tttatctcca cctgcattga tgtaagactt ttcgatcact
      481 ttcatttcga tccgtccttt gctgccattg aaagaaacat tgaatccttc ccacggcata
      541 tacgcgttta gcgaatacgt caaaatcgca ccagtttcat atttgaccgt tacgcctagt
      601 gtatcttcaa tagagatatc atcggaaaat acactgcggt cacgaatata tccactgtct
      661 ttttctgctt ctaaatacat ttctcgcaat tcctcattgg ctgacaggtc tatagcaaat
      721 ggatcagctt tagctagttc gcttccatag catcgactgt aaaatatttc tactccgcgt
      781 ttctccgcat tatgcatccc ataaaagttt aatccaccga atgcaaatac cttttgggga
      841 cggctgttca accaaaaatt cactagatca aaatgatggg tagatttatg aacaagaagc
      901 ccgccgctat tgtgcttatt tcgatgccat cttctgaaat aatctgcccc gtgttgcgta
      961 tttaacagcc attcgaagtg gatagaaaac acatcaccaa tcacatcact ttctatcagt
     1021 tcctttatct tcgtgtgata cggtgcataa cgataattaa aagtgactcg caggcttctt
     1081 cccgtctgtt tttggaccgt tattatttct tgagcttttt cctcatcgat agtcagaggt
     1141 ttttcgcaaa taacatcata tccttttttc attgcacgaa tgatataatc atgatgcgtt
     1201 cgatctactg acgtaataat gacttcagcc ggccgtacgt catccagtag ttgctcaaat
     1261 tgtgatggca tacatgtctt tacttttttc tgtccatact tgttcacaag tatccgattt
     1321 gcataatcca tccttgtttg agatacatca caaaaaccga ctagttcaca agtttgatga
     1381 tacgtagagg caattgcctc ataaaaaaaa cgcgctctcc caccaagtcc gacttgtaca
     1441 tattttttct tcatttaaag tcattctcct tttcttttaa gcaaaatact gtgacaaatg
     1501 gcagccctgg atacagtaat gcagtgagta aaaaatcatg ctgtatacac cagaacagtc
     1561 catacaaaag gcataaatac cacagaaacc ccttgacgtt ttttgacaac agccagatat
     1621 tccttttgat caaataaaca agactatctg ctttatcttc tttgcagata tcaattagcg
     1681 tactaaaaaa actatgggat acaaaaagca acagacacaa tagaaaagga atcagatacc
     1741 aagctccaat ctttcccact aataaatacc aatcaactgc aaaaataatg acaaacagct
     1801 gaaacaacgt aatgatgaaa cacatccgtt tgatttttcc tttcctccaa catacctttt
     1861 tttctgccaa gtaatggaca gatagataat aagaaggata catcatcaaa aaataaatca
     1921 gatagacaac acgagcagac agcactaatc ctaaagtgaa caaacatcct agaactaaag
     1981 cgaaaagagc attactgata agactggtta aaaaaatccg acagacatag tagttgagct
     2041 tgtaaagaaa catctcttct attggctttt tcatattcag cccttcagac cgcttgtact
     2101 gatcccctgg acaagatatt tattgaagaa taagaaaatc agaaaaaccg gtagcagcga
     2161 caacgtagac atcgcaaaca tactcgcgta agtcgtacta gatccagcgt ctgcaaataa
     2221 tttgatggca atcgataccg tgtaagatct cggcttgttt aaataaatca atggtcccat
     2281 gaagtcgtcc catttccaat aaaactggat gatgatcgtc gtgatgatcg aaggtttcag
     2341 caaaggaaaa ataattttgg taaagacact atacttgctg catccgtcaa taatcgctga
     2401 ttcatctaac tctttgggga tgctgaccat gaattgcatg atctggtaaa tgaaaaatgc
     2461 ttgtccaaaa aaatgtggta aaatcagcgg cacatacgtc ccaaccaaat tcagccgatt
     2521 gtaaataata aactgcggaa tcaaaatcac ttgtgcagga atcatcatcg ttactaacat
     2581 gacgacgaac cagaatcttt ttcctttgaa atcgattctc gataatgcgt aagcaataca
     2641 agtcgagctc agaactgtcc ctatcgtcga aactgcagag atgacaaacg aattcctaaa
     2701 gaaagtaaaa aatgaaattc cagcaaaccc cgcccatcct tttgcgtaat tttcccattg
     2761 aaaagtctct ggaatcaaat tcagcgaacc actcataatc tcttgatccg ttttaaaaga
     2821 acctgcgatc atccataaga ccggataaat catgactaga cctaatagca aaataaaacc
     2881 gtattgaaac catttcgtta ctgtattatt catttcttcc tcctctcatt cacacaacga
     2941 cccatttttt tgatgatttg aaaatcagaa aggtgacagc tcccataatc aacagcatga
     3001 tccacgaaat agcactagca taccccatag aattaaaaga aaaagcttgg ttatacacat
     3061 aaagagcata aaagttcgtg gcatcattcg gaccaccttg ggtaatgata aatgcctgcg
     3121 taaaagtcat aaacgccgcg attgtctgca taaccaagtt atacaaaatg atcggcgaaa
     3181 gaagaggtaa cgtgatcttg taaaacgtct gccatttgtt cgctccatcg atctctgccg
     3241 cttcataata cgagcctggg atctctttga tacctgcagc aaagatgatc attgccgaac
     3301 cgaattgcca agcagacatc aaaacaagag ggatcatgac cagtttttga tcgctaaacc
     3361 aggaaagcgc aggtgcgcct aatcgttcca ggaaaatatt cactaacccg tttttagaaa
     3421 acaactcccg ccaaactaga gcgatagaaa tactgccacc gatcaaacta ggcacataat
     3481 acaaagaacg aaagaaagtc gttcctttga tattcctcgt taaaagataa gcaatcaata
     3541 gaccaaagat cagtttcaca ggtacagatg caaatacata gagaaaagtg actcggatgg
     3601 agttcaaaaa acgggcatca ctcagcatct ttatataatt atccaagccg atccatgaga
     3661 ctggatttcc caaacgataa tcggtgaacg aataataaag tgacaagatc atcggtatca
     3721 ctgttatcaa tagaaatcct aggatgaatg gagaagcaaa tacgtaaccg accacattgt
     3781 cttgatccaa gaaccgtttt agtttttctt ttttcatgcc ttcctcctgc tgctatccta
     3841 gaatgtttga cgcaaactca taaagactct ctgctgcttc ttctgctgtt acttcttcat
     3901 aaatgacttt ttcaactaaa agattgtatt ggtcttgaat ttccggattc aaagcactat
     3961 caaagacatt cacttcgccg ttgtccatat cggaaatcat ttctaaataa tcaaatgtcg
     4021 cttgcaaagt tgcatcatct gcacgagcga gctgttctcg aatattcgac atgattggta
     4081 caccacgttc tccttttaaa atttcattgg cttcttcact gttttgaaag aaatcaataa
     4141 acttagcagc ttcttcttta tgggctgagc tttttgccat actcagcatt tgcgaagaac
     4201 gtaaagggat accagattct ccatctgctt ttctgcgagg tggattaatc aactgaatct
     4261 ccatatcttc aggagcccct gacaatattt ctgtcaactg gttacttgat agaaacacca
     4321 ttgccgcttc tctatcccta accgcctgaa aagacagatc cgtcgtactt gcagctccac
     4381 cacctggttc tggatgggcc cctttgtcta ccagattttt acgcagctgg aaaaaatctg
     4441 ttagatagtc tggattttca aatcccaagc tgttagatag atcttcccga ttataaaaat
     4501 tcaatccttt ctcaacttgt ggtatttgaa taatacagcc agctataaaa tcttcgaatc
     4561 ttgaaatagc atagattcct aattcttcag tgatttgata gctgatttgt tcaaattcat
     4621 cccatgtcca atttgaagct ggttcaggga tatttaattg ttcaaatata tttttattgt
     4681 aagcaatcgc aaacgaattc accccgttag acagtccaac taattgcccg tcgtaagtcg
     4741 tcgttgctaa ataggattca tctgcattag aaatatcaat tatcccttgc tccacatatt
     4801 catctaatgg ttcaatctga ttgatgtatt gaggaaattg attgttcagt tggaaaatgt
     4861 cccagatatc atctgatgct gctagtgtac ctaattttgt gtaatatcct tcataatcat
     4921 aaaattcata atcgatcgtc acatggggat ttttttcttc ataaagctcg attactttta
     4981 ctgttgcatc gtgtcggact tgcgaacccc accaagccat gttcagcgtg atttttttgt
     5041 catcttcttg cccacaagcc gtcaagaaga ataacataac acttaaagct aagatgattc
     5101 tcattctttt cttcattctc tgcacctcta cttccattgt tatttttcaa tatactgtat
     5161 ttttatacag aacatattta ttaaacgatc tttttcaagg ggaataaaaa ccaagacttg
     5221 ccttatcgtt ctgcctagga tgaacaactc aatactttca agaaaaatta gatagtgctg
     5281 attcttcttt catcgctaaa aacacttctt gcttcaatga tgggaggata attacgcaaa
     5341 gaaaaaagga tatcagacaa ctgcccaata tccttgacat agctaaataa gcatgcttct
     5401 tcgtgtgcgc tcttccctaa aaaacagaaa aataactagt aattctgtat gaaattaggt
     5461 acaatataca cgtcgatgca caagcattga ctatggcaac ttgattggtc agttcccgct
     5521 gacttttcaa gccccgacaa tttagctgct actaaattgt cgggtgcctt ttttagtttt
     5581 tattcgtttt tcattctata gaaagcgctt caataaatca agacattttt ttgttagaat
     5641 gctaataata acaaaaggtt ttaactcttc ttatgtacat tacaggtaaa aaaacaaaaa
     5701 aatttctctt ttttttgaat tctgaacaaa tagtactgga gtacaaaaga cgaggaagag
     5761 ccactatctg ctcttcctcg tcttttgatt ctcacctgtt attcatgatt ctttttctca
     5821 gtttcccgat atttttccat tttacgacta aaaagctctc cgctacttgg tggcgtatat
     5881 gtgatggcat ttgctcctgc ttgaatggtc tcagcaatac tttcttccgt tggaccacct
     5941 gtcgcaatga taggaagttc aggatatttt ttacgaaaat ggcgtactgc ttctgcagtt
     6001 tctttacctg cactgatatt gataatcgaa ataccagctg ctaacttttc atctatttct
     6061 gtatatttcg acgttaccgt actgataact ggaatatcaa ctactcgaca aacttctttt
     6121 actgtctcta aaggcgtagg accgttcaaa acgaccccta acgaaccttg cgcttctgcg
     6181 aacatgctca tgtaggaaga acgagcacct tgtgtcaaac caccacctac accggagaaa
     6241 actggaatgt cagctgcctc aataatgctt ttagtaatcg ctggatgagg tgtaaaagga
     6301 tacacagcaa ttactgcatt cgcatttgta ttacgtatga tcgcaatatc cgtagtaaag
     6361 atgatcgaac ggatcaattt tccaaatatc ttgatcccgc ttgcttctaa aatgatttcc
     6421 ggtactctta cgatatcttt tctcaattcc gtcatgattt ctggcgtaaa ttgttctgcc
     6481 atcagccact tctcctttgc tatctccggt catactgatt caattgatac gtctcgatga
     6541 cctggatgat tttttgcgca taaccggggt ctgtcgcata acctgcatct tgcaatgctt
     6601 gtgcggcttc ctgatagttg gtggccgtaa tcactttttc atacagcgca ggattccaat
     6661 ctactccttg gacgaacagt tgtgtatggt catccatgga ctgttcccat gaatcataca
     6721 ctttgaagtc tccttggata gttatccatt cttcgttaac aaattccttt gtttccaggc
     6781 ttactttctt ctgatcccca taagccttga tcccgaaaag attattgtat ttgctagcca
     6841 aggtactttt tccccaattt gattctaata tagcttgtcc taaaatgata ctaggaagca
     6901 ctccataacc atcttgtagt tcttgggcat gcggaacaag ccgttcaata aattgctgat
     6961 gggattgcgc ttcattttct tgctgattat cagtaaaagg agaagacaga ttcttcaagg
     7021 aaaaaacaaa ggctaagagt atcactgcta caccggcaac cactgctggc caacggatat
     7081 tcttgcgctt ttgctttttc cacttttttt tcgccatgat caattcctac ttttcattta
     7141 attcattcag atattcttct agcgtcaatt ggtgtttggt gatatactca gcgttttctt
     7201 taccgacata acgtaagtgc caaggttcat atgtgatctt agttatatct tcgtgtcctt
     7261 ttggataacg aacgataaat ccatactgcg cagcattatc tgcgatccat ttagctccag
     7321 gctgatctcc gtaacttgca tccaatacag tagaaggata actgttgtac cagttttgat
     7381 cgacgacatc gactgccaga cctgtgtggt gttcgctgta tcctggctcc gtaatcgttt
     7441 ctttagtaat ctttgtcgcc tcctcttctg atacaccttg acgactcatc acatcttgga
     7501 cattttgttc gaaaacttgt tgttgggatg atacagaccg gtaagctgag accatcacta
     7561 atggaaatcc agctgcttca gcagccttgg caaatgcttc atattctgga gcaatgcgtt
     7621 tgtcgatcaa gtatccgtta cttaatgatg ccaattgagt agactcatca atttcttttt
     7681 ctaatttgtg atctggacca accaaaacta gcttccaatc gtctgtttta acatctggca
     7741 attgtttgga cgtacgatct tttgtttctt tgtcctttac cttgctgctg gttgtttgac
     7801 tgttcttttc tttactagca gttgttttgg tgcttgttcg ttcgactgct tgattgccgg
     7861 cttgaacatg cgaatcatca tcgctaaaaa taacatagcc cattgctgcg atcataatca
     7921 caactgctcc aaatcctaaa aatttattca cttgaaaaat cctttcgttg ttcttcacgt
     7981 tccccattcg cctcatgaac gtaagtttct tccagatttt cattcaccca atcgagaagt
     8041 tcgatttttt ctccttcgat ctgtttttga acatctgctg gcagacgaaa attcaaaata
     8101 tcatcatagc cccattcatc atattgaata cttactttca agtcaaggta tctaccagcc
     8161 tgctgattat ttttcaaagg gatcagctcg acttttgctg ctttgctgct aagccatttt
     8221 tgcgcgatgc gtgttttgat ttgcttgtac tctgcaacgg caacgtttcg tttttcggga
     8281 aaaataatcg tttgacgcaa aatcttttgc gttagcatcc attcataatc agtgaaaaga
     8341 ttttttactt tctgcatcgc ttcagatggc agatttttct cgccatcttt ccatttttgc
     8401 cattgttctg gactgatcag caagtattca tctaaaaaat ttttttcagt ttcaaactgt
     8461 tctaataata cactcataat aagatcaata tatacttgat gcacgtttat cccctccttt
     8521 taactattct acttaagttt tgatcaaaaa gcacctctta aatcgctgct ttcatttttt
     8581 ttaatgtttc aagtcctctc ctctttattc ttcgtgattt ttgttataat aatagacaga
     8641 caagaaactc aatccaacta tgaaggagcg ttttcagatg aaaaaatatc aatggggaat
     8701 cgttggtctt ggtaccatcg cacatgagtt tgccgaaagc ttcaatcaag agacgagcga
     8761 actaacagca gttgcttcca gaacttcaga aaaagcggaa aacttcgctc accgttacaa
     8821 tattccaaaa gcttacggaa gctatcaaga aatgttagac gatgctgaaa tcgacatcgt
     8881 gtacattgct gtaccaaata gacagcatat cgaccatatc ttggcagcat tggaagcagg
     8941 caaacacgtt ctatgtgaaa aagccatcac gatgaataaa aaagaactgg ctgacgctat
     9001 gcgcttagca gaagaaaaga atttgatctt agcagaagcg atgacaatct tcaacatgcc
     9061 actttatcag cagttacgct caataatgga tacaggaaaa ctgggggctt taaaaatgat
     9121 ccaagcacct ttcggcagtt acaaagaacc agaccctaaa aaccggttct tcaatccaga
     9181 attagctgga ggagctctgt tggatatcgg tacgtatgct gtatctttcg ctcgtttctt
     9241 tttgagctct caacctgaag tagttgcctc tacaatggtt ccttttgaaa caggcgtgga
     9301 cgaacaatcc gtcactatct tgcgtaacaa agaaaatgaa cttgctgctg tcagcttgac
     9361 ttttcaggca aaaatgccaa aagtcggtat cgttgccttt gagaacggtt atgtgacgat
     9421 tgctgattac ccgcgtgctg atcgtgcgga gatcttattt acagatggta caaaggaatt
     9481 catcgaaagc gggaatacat ctgaagcaat gaattacgaa ataaagaata tggtacacat
     9541 gattgaaggt gacttgccca atcgctctct cttcttgaca aaagatgtca tcgaaatctt
     9601 ggatcaaatg caagttctat ggaaaaatat gtaattttaa aaggttgtga aaaagctgtt
     9661 ctgcatcaag taataagaac agccaaacaa aaatagctcc tcctattttt cgtttggctg
     9721 gacttattac cgcagaggaa agcttttgaa caccgtttaa gaaagaggtt gtgagcctac
     9781 cgttcagctc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt ttgttgaata
     9841 ttgtgacttg tgaccgagga gttgacagga gaacaccgtt taaagaagag gttgtgaaat
     9901 cagctttttg acctgaatat tagctgaatt cacaacctct atttttggat tttttgttgg
     9961 gagaattctg ccttgatttc aataaaactt ttaaaagact gcaaaaactg gaacaattct
    10021 gctcgatcct cgaattcagg gcgactagtc ggcagaggca tttgccgata caatacataa
    10081 acttcttcaa tacgtgttaa aatttgtctg ccatcatttg actctgaaaa tgtttctgca
    10141 gtataaatca ataaaccata gatatgtttt ccataataag ctggttgctg gattctctct
    10201 agattttcca gcatgtcttt aatcacattg gcctgtgctc ttctcataga gaagtaagtt
    10261 tcgtagtata gcggctgtct gagccagtag ttttcttgat gttctctggc atcttcttgg
    10321 ctttcgcgaa tgaatgtcag taaatgttcg catttgatct gcacatcttc tttattttct
    10381 gataaaagaa attctgccat ttgtctcaaa atatttcgaa attctttttc gatctgtatt
    10441 tggttttcct tcagctgctt cgtacgatct ggcatgtaaa catttgcaag caaagcaaat
    10501 actaatccaa tcgccattaa tcccgcttca ttgccgatca gctgccaaga catgttttcc
    10561 tccactaaat aatgagtgac caaaaccgaa tttactacta tcccttcggt taatttgaag
    10621 cgagaggcaa gcgggatgaa caggaataaa aaaagactga agccaaaaat agtataccca
    10681 acagcaaaaa aactgaacag tgctgttgcc gtagctaata taaaggctgc gattcgattc
    10741 atgcccgtac gcaatgtaga gcgcttggta tttcctacac ttaataatgc aatgatccct
    10801 gcagctggcc aatacaacag cgataagcta cccgctgtga ccatcgaaaa gaagacactg
    10861 atcactgttt tgactgttcg cattcctata cgcatttact tccccctttt ttattttaat
    10921 tatacttgaa taatgaaaaa ctagctatca gacaaaagtt taggtatgat agtaaaaaga
    10981 ccaaaaagga gaatctctat gcaatttggc cgttttcgac taggtatgcg tacgatcaaa
    11041 agtgccttag ctgttttttt atgtatcttg ttttttcatt tgactcaacg aggaatcccg
    11101 atgattgctg cattatctgc tgttttttct ttgcgtcagg acttgactac aaccgtttct
    11161 tttggaaagt ctaggatcat cggcaataca ttaggtggta cattagctat catttatttt
    11221 tttgtcaaaa gctattttca caatgatttt ttggtagaat tgatcttgct tcctattcta
    11281 gttattattg tgatcgtcgt gtcagatggt attaacaata attccggtat catttccgca
    11341 atagctacgt tgttgctgat tgcgttgagc atccctcaag gcgaatccgc tttgtatgct
    11401 atccaacgtg tactagatac ttttataggg acattcattg caatcggtat caatttcttt
    11461 cttcgtcctc ctgaaacaga aaagaaggaa gaactatcag aagatttggt cgaactccaa
    11521 aagaaagaac gattgctaag aacaaattta caagagatag aagctaagat ccaaaaacaa
    11581 aagaaaaata gcgaggacta attttctttt gcaataaaat aaaaaaatgg agagaagaac
    11641 tttaaaaagt cttcctccat ttttgattga acggtgaagt gtactacttt attcttcctt
    11701 catcgagttt tgttctgcct cttttggcgc ttcatacata tttacttggc tagtagtccc
    11761 acgtgtcgac aaattctcta cgatttcctt caagtcaagt cctgttgtcg cttttagtgt
    11821 ttcttgagta gaagaaagca aatttgtggc ataatttgtc actcggttcg ctccactggt
    11881 ttctcctgaa ctgcttcctg tatcgacaac agagattttt tcgatatttc ctagcggctg
    11941 tgctgcttcg cgcatcaatt gcggtagcat atcgataacc atactcaaga cagctgcttc
    12001 gccatattcc ttgaaggcat tggcgatctt ttgttttgct tcggcttctg cttttccttt
    12061 tgctagtgct gcttcggctt ctgcttgtcc agctaaacgt gtcttattgg cttccgcttc
    12121 tgccagtgct tccacacgga atcgttctgc ttcggcttca gtcacttctt tgactttttg
    12181 agcttgtgct tcttgttctt tagcgtaccg atcggcatct gcttttttct tcacttccga
    12241 atcatattgc ttttcacgac gtgtgatttc tttttcttcc agttcgattt gtttttgacg
    12301 ttctacgatt ttaatctgca tttcctgttc gattacttgt tgttgtgctc gggcactttc
    12361 taaattgtag gcttggtctg cttttgcttt agcaacatct tgttcttgtt tgtaggttgc
    12421 taatttcagt tctttttctt ttaatgattc tgcgatctct gtttgtcgct gtaattcagc
    12481 tgcttgtgat tctttttctg cttgagcttt tttgatacgc gtttctttta gagcttccgc
    12541 ttctgctata tctgcatctc ttttgacttg ggcgattctt ggtttcccta aagagtcaag
    12601 ataaccgttt ttgtctctga cctctttgat agtgaaagaa acgatgatca accccatttt
    12661 tgcaaggtct acagaagcga cttcttgtac gctttgactg aacttatcgc gattttgata
    12721 gatttcttct acagtcatag aaccaagaat cgagcgcaag tgtccttcta atacttctct
    12781 tgcttcattt tccagttctt ctcttgtctt gcctaaaaac tgttctgctg ctgtagcaat
    12841 ctcttctaca gatgaaccga ttttgatgat cgaagtacca tcacacatca ccggtacgcc
    12901 ttgttccgta tacacttcag gggtggagac gtccaattta ctcgataata gactgatccg
    12961 attcgaacgt tgaaaaactg gcaagacgaa tgcgccacct ccacgcacaa ttttgatttt
    13021 gttattgctt tcatcaatgt gcacattttt gcttcctaaa taacttccac tgatgatcaa
    13081 cgcttcatcc ggttttgctg tctggtactt cgctacaaag acgatcagca acatcagtaa
    13141 aatcaaagct gcgattgcga tcgcgataaa aacaactggt atttccattt aacacacgct
    13201 cctttaagct tttatcatgc tttcataagg catgacataa cagattcttt ccttgatctc
    13261 aatgatcaat actttcgttc cccgctcagc tgtctgttct tgggggctgt aaaaacaagc
    13321 tggacgacta atggaaccgg taacactttt caactgaatt tctcccatac ctcgtacagg
    13381 gatcggtgtg atcacaacag ctacttgtcc ttccagagtt ttttcagaag acgacatcgt
    13441 tgattcggca tgtttcatcg gcatcaatat atagaaatta agcaaaaata ccaacactat
    13501 cgatatgcct ccactagcaa taaataaaaa taaagatgat tgatctgtta aacgctcacc
    13561 tagaaaacca aataagcttg tgaaagttaa ccaagggata ataagcatcg gatcaagcgg
    13621 gccatcaaaa tcgaatacat ctccaaaaat aatcaatagg actgccaatc ctgcacaaat
    13681 cagcaatgta taccaataaa tcgtctcaag tgcgattccc cctatcatat acacgcctcc
    13741 ttccatttct acgtctatca taccaatgtt tgaaaattct gtatagcttc ttttaccgac
    13801 tcagtggtgt tttttatagg aaggttgtgt ttatcgttga caatttgtta aatttttgtt
    13861 ttcatgttaa aattttcaca ataaattctt tcgaggtagc ctatgtttcc tttttttaag
    13921 aaaaaaccat taaagctcca gaacacgatc atccttgtcg tttgtttctc cacattcgtt
    13981 tctttgattg tttctgccat attgatcagg aattttattc tttcgatgaa ccgatctagt
    14041 gtgtcagata aaatctctag cattgctaaa atcgttgcca atgataaact catccaaaaa
    14101 aagctgcaga ctggtgaccc gacacatgcg attcaaggaa taacaaatga aatcatgatt
    14161 gcatcgaatt tagattttat cgttgtcatg gaccctgaag gcattcgata ttctcacgca
    14221 gacccaagtt acatcggtct tcctttctca agtcatgaag atgtgaaaaa agtgattgga
    14281 ggacaagaat ttttctctga aaaagaagga atgttagggt tcggcttccg ctatttcgtc
    14341 cctatttatt atgatcaaga gattattggt gcagtttgtg ttggcttaac aaacaacacg
    14401 attgacaatc aagtcaaaga tggacaaaaa atgattgttt tttcattgat tattggactt
    14461 agcgtaggca tcataggcgc cgtttttctt gcccaaaaaa tgaaaaaagt actgcttgga
    14521 cttgaaccac aagaaatagc agaacagtta agtgaaaaaa cgattatcga aaatgaagta
    14581 gatgaaggaa tcttagccat ctcttctgat aaaactatcc tcttgatcaa tacacctgca
    14641 aaaaacattt tctaccaaac agagcagcta ttggatttcg aagtaggaaa aaaatcagtg
    14701 ataatctata tgacgttttg tttaaagaga cgattgagca tcaaacgacc gttcggactc
    14761 aatccatgta tttaaatggg atagaaacta tttctagcgt tacacctctt tttagcaatg
    14821 accgtttttt aggagcagtc gcaactttac gaaatcgatc agaagtaatg aaattaatgg
    14881 aggagcttgg ggggacgaaa caatatataa actccatgag agctcaaacc catgaattca
    14941 tgaataaact tcatgtcatt tctggtttga ttgatttgaa gaagtatgaa gaagtcaccc
    15001 attttatcca gcagctaaat caaaattata aaaaagagat tggccaagtc tcaagtctga
    15061 tcaaagtacc tgcaattgcc ggttttttat taggcaaaat aaacgaagcc aaagaacaat
    15121 ctgttgaaat gctgttagaa agcgattctt ttatccctaa aatggagatg gatgactcca
    15181 tccattcact tttgcaagtg ttagggaact tacttgataa tgcaaaagaa gccgtgctac
    15241 aaaaacatga aagaaacgga caggtccgga ttcatttaaa ctatgacaca gaagctgaag
    15301 tattcatcgt gaccgtctgc gatgacggtg tcgggatatc agaagaaatc tcagaaaaaa
    15361 tatttacgct aggtttttcg acgaagggaa tcaatcgtgg gtttggattg aatctcatcc
    15421 attcaatcgt caaacgccac ggtggatttt tagaattttc caaacaaaca aatggcggta
    15481 caatcgtcta tctagaatta ccaaaaaagg aagatcaaaa tgatgacaaa tgtcctgata
    15541 atcgaagatg atccaatggt tgcagaattg aatcgtactt ttgtggaaag catccctaat
    15601 atgaacgtaa tcgaaagttt cagaaatgta gaagaaggaa aaaaatattt cagacaaaaa
    15661 aacgcagatc tgctgctgct tgatgtatac atgcctggcg aaagtggtat ccatttttta
    15721 tcttggctta gacagcaagg aaatgatatc ccagtgattt tgatcactgc tgcagatgat
    15781 atcgaaacca taaagaatgt gatccgctac ggtgccttag attatttgat caaaccgttc
    15841 actttgcaac gttttcagct ggcgatcgaa aagtatcttg ctttctctca gacaatccaa
    15901 acgactgaaa aagccgatca gaaattgatc gatcaagtat tcaaccacac cactcccatt
    15961 cacgaaatac catcattcat cgatttgcct aaaggtctgt caaaactgac tttaaaaaaa
    16021 ataagtgcat ttattctaaa agaagatact attttttcaa caaaagaact ggctcaaaaa
    16081 agctcactgt cacgtatatc cgtcaagaaa tatttgaatt ttttagtaga acaagatata
    16141 ttgaacgaaa gaatcaaata tttggaaatc ggccggccgc taacctgtta tgaattgaca
    16201 aagaatgggc ggaaaaagct cacagattac tgttgagtca tcgaattatc ccatacaaaa
    16261 tagtccaagt atacagaagt aacaactgtt tacttggact atttttaaac attttattga
    16321 cgaatatcca tgttttctta tcttaattga cgggctttag gaagagatac ctcatttttc
    16381 gtttttccag tttctattat ttcacgaaag ttctcgaaac ttgtcgaaat catatttgat
    16441 aatgcctcat ctgtatttga acctacatgc ggggttacaa gaactctagg gtacatttct
    16501 actaattgct ggatagcagg gtctggaatc gtctcccatg gcttgaagga tcggaaaaaa
    16561 acctctgttt catttgcaaa aacatcggtt gcaaaaccag ctaaatgatt gcttttcaaa
    16621 gctcttaaaa tagcttcatt atcttgaagc tctccacggg ctgtattaat caagatgcta
    16681 ccttttttca tttgagctaa aaatccttca ttgatcattt gatcgttttc acctgggata
    16741 tgaggtacat gaatactgac aatatcgctt ttggctaata actcttcaag cgagtggaac
    16801 gtcaatactt ttttagcagc ttctgtctga tgaatatcgt agccgatcac tttggctcct
    16861 agtcccttaa acaaaacagc ttccgtcaac cctatcctgc ctgttcctat gatccctact
    16921 gtgcaatttc tgatttcacg actaaacatt tgctcatcca ctcgaaaatc tttgaaagat
    16981 gttcggatcg tcgtatacgc agtatttctc aaaagcatca tggcaaaagt caaagataac
    17041 tctgctatcg catttggcga atacgatggt acacgggcta ctgtcatacc aaaatcagct
    17101 gccgcttgta gatcgatatg attgactccc acagtccgtg taaaaacata acgaatccca
    17161 tattcctgca tttttgcaag attggtctca tctgctatac aatttcccct caacaataca
    17221 gcatcatggt cctttgctga cacgatattg tcatgtgtca acagttcttc ataaagactg
    17281 agatcataat ggtaaatatt caatttttca aaaaaatcta tctcgttcgg acgaacaccg
    17341 taacatgcga ttctcatact ttcttcctcc atttcttatg aaaataaacc tatgccatga
    17401 atcatttgta atacaacgat ccatacaggt gtcagtacca cagcaaaaat agttgacagt
    17461 aaggatgcat tggaggctaa aagtgcctcc ctatcaaaac tgatagcata ggctgctgcc
    17521 actgttgcag ctggtgtagc catcatgata acgattgttg ccagtgcttc aaaattgact
    17581 ggtagaatat tcgttgccgt caaaactgct agaatcacga tattcaatgc aggtacaaat
    17641 accactttga ctagactgta ataccatgaa gttttttcag atgcagcttt tttgaagctt
    17701 acttcaccta atgttgcgcc gattgctagc cacgccaatg gtgaacttaa accagctaaa
    17761 taatccatcg gctgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa cgctacttgg
    17821 cttatctctc cttctgcacc tgtaactgac acttgtggca gatacccttg gaacagccaa
    17881 atgaaaaggc cggcaaaagt agcgataatg attggattga cgaacatcgc tttgatattt
    17941 ttagcttcca tcttcaagcc actcattttt atgtatccat aagaatacaa aaagattcgg
    18001 tagccaatat tgaaaattga ggaatacatc acgccgacag gaccataaat cgcactgacg
    18061 ataggcgttc cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa cgttgtttgc
    18121 ttatcccctt tatagcttaa gaaaaaaggt tttgaaacta gaataagaat aatataaata
    18181 actaatcccc aaatcaagac attgattcct tgcatcagta attctgtatt gatatcttgc
    18241 atgaaagctc gaaatgccag tgctggcaaa gccactgtta agacgacttt cgatagaatt
    18301 tttccaacag attcagaaaa aatccccttt tttctacaaa caaaccctaa taatataata
    18361 aaaactgttg aagtgattgc actgacaatc gacatatctg tcaaagtcga tcgaaaaatg
    18421 tcaaataagt tcatctttct cctcctaatt atgttcattt attcacaata aaaaaagctt
    18481 ttattttgct aatacgtatt aacattttta attttatctt tacattgtta cttattgaac
    18541 aataggaaat taaagaaact tttgaaactt atgtaaagtg atttttacta gaacacaaaa
    18601 aaaaccttaa aaccaaactt aagaagttta gttttaaggt taacttaaat tatattatgc
    18661 gtggaaacgt tccacgccat ctagataagt tgcttccaat gtcatatcag ggttcaatac
    18721 gataaagtct gcatcgcgtc cttgagcgat ttttccacat gtatcatcga ttttacagct
    18781 gattgctgga atcagactag ccatcatgac tgcttgttct ggtgttgcta atccccagtc
    18841 tacaacattt ttaattgctt ctttcaattt caatatactt ccagctaagt tgccagattc
    18901 tagacgtgct gttccttctt ttactactac tggaaattcg cctaaattgt aatttccatc
    18961 aggcatccca ccggccatca tacagtctgt aattaatgcg acatgatcat gtcctgcttt
    19021 ttccattagg attcccgcag catttggatg tacatgatgg ccatcacaaa tcaattcaga
    19081 aaagacatgt tctaaagata ataacgcacc caccatacct ggttcgcgat gattcaatcc
    19141 acgcatacca ttgaaagcat ggacaaatac acttgcgcct gcttcaacag ctttttgcgc
    19201 ttcttctagt gtagcattac tgtgtcctag agaaacaact actccttctt cagtcacttg
    19261 tttgacaaac tcttctacac cttgacgttc cggtgctaaa gcaatttttt tgattaaacc
    19321 acctgaagct tcttgccatt catggaaagt atccaaatca ggatccccga aataacttgg
    19381 gttttgtgcc cctttgtgtt cttctgtgaa gaaaggacct tcaaaataga ttcctttgat
    19441 tttagctcct ggtgcttcct gatacatttt gccgattgtc tcagctacat cttttaaacg
    19501 ttctttgctt gacgtcaatg ttgttggtaa aaatgaagtc actccacaag ataacagacc
    19561 ttcagacatc actttgatgc cttctgcatc attatccatt acatcatgat tcatatagcc
    19621 gtgaatatgt gtatctacca aaccaggagc aatccatttg ccgctttggt caatgatgtt
    19681 gacttctccc tcaggtagtt ctttggtgta ttcaccaaaa agtccatcgg taatttcaag
    19741 atagcctgca tgtttgacac ctgacgctaa aaagaatttg tcagcataga taaaagttct
    19801 cattctattc tctccctttg tttttctacc tctaaagtac tcttatttag agaagaagtc
    19861 aagaatggtc tacaccattt acaaattttt tgaaatttgt ttcacagctt gtgctaagcc
    19921 ttcatagaat gctgctcttt ctggttcttc tttacgcttg tatattaagc ttttaagcac
    19981 aaataaatca ttgaggtaat agtagctatt cttctcttga gcccaataga ttccttgatt
    20041 cacctgttct aacgcttcgt cgatttcatt ttgatggaat aagaaatttc cataattata
    20101 aaaaatttgc gaaaattcac tttttgatcg ttcatcagct gattgatgca gcagttggat
    20161 gcttcgactc aaatagtagc gtgcttcagc cgtttttcct gccacaccat atatttttcc
    20221 gatacaacta atcagttgta tttctgtgtc agaaacatgg attctttctt tttggctaga
    20281 gtagcttaat gcttctttca aataataaag ggactgagag atattttgac gcaacgtgaa
    20341 ttcgcaaaca ccgagataat aataatagcg ttgaaagtca gttgcttgat acagattttc
    20401 agaaatatca gaagatttta gtacttgttc taacttccga taatcacgtt tttggaaaca
    20461 actatccaac agttcaaata atttatcgtt tgtccgagta gtttttcgag agagattcat
    20521 cacatgatca atcgttaccc ctagccgttg acaaatctgc atcatcacca atacattcgg
    20581 cagttcctca ttattttcga tccgacttaa tacactttgt gaacaaatat catgcgaaag
    20641 cattttttgc gtcatttttt gttcttttcg aatctcttta atgatcgttc caaaggactc
    20701 gattaacgtt tccataagta tcctccccaa aatatgcgta ttcgcattct attcgtattt
    20761 tactttagga agtcgcaaac ttcaagaaaa gacttgcaac taaataatat actttagtta
    20821 tttaagaaaa ctccactaga aagagagcct cagtgtgttt gtctgtggcc ctcctactga
    20881 aaaatttggt ttttaaaatc atttttggcg ataggttgct aggaattctc tcattttctt
    20941 agctgtttgt ttcaaatcat ccattttagg aagataaacg attcgaaaat gatctggctg
    21001 gttccaatta aatccaccac catgtacaag tagaatatga tgttcatgta aaaagtccaa
    21061 aacgaatttt tcatcatcca aaatattgaa tcgctttgta tcaatttttg ggaatatata
    21121 aaaagcagct tttggtttta cggctgaaag accaggaata tcattgattg cattatagat
    21181 atattctctt tgttcataga tccttccgcc tggcaggagc agctcatcga cactttgata
    21241 accgcctagc gcggtttgga taatttgctg agacaataca ttggaacaca aacgcatgga
    21301 ggacaacata ttcagccctt ctatatagcc tttgacgtgt gacttatctc cacttaggac
    21361 catccagcct actcgaaatc ctgctacacg atgagatttg gatagaccgt tcagcgtaac
    21421 aacgaacaaa tcaggcgcta atgtagcaat cggaatatgt tctagaccat ccatcactaa
    21481 gcgatcatag atttcgtcgg aataaataat caggttattt tgtcgagcta cttcaacgat
    21541 ttcttctaat aattcttttg gataaagtgc cccagtagga ttattaggat taatgataac
    21601 aatagctttt gtccgactcg ttatttttga tttgatatca tcaatatcag gataccattc
    21661 tgcctcttca tcgcaaatat aatgaacagg tgtgcctcca gctaatgcaa tagaagccgt
    21721 ccacaacgga tagtccggca ttggaaccag tacttcatct ccattgtcca gcaacccctg
    21781 catacacatc gtgatcaatt cgctcactcc gttgccggtg tagatatcat tgatcgtcac
    21841 attcggaaaa tgtttcagtt ggcaatattg ctcaattgct tttctagcag aaaagatccc
    21901 cttggaatct gagtagcctt ctgagtcacg ggcattcatg atcaaatcac gaatgacttc
    21961 gtttggtgct tcaaatccaa aaggtgcggg atttcccgtg ttcaatttta aaatactaat
    22021 gccctcttct tgcatccgct ccgcttcttc tagaacggga ccgcgaacat cgtaactgac
    22081 accttctaat ttgttggatt tttcaaattc tctcatcact tagccaatcc ctcatttcaa
    22141 aaagttagaa ctttccaaat tttttgttat ttactatttc aatatcatac tccatcctag
    22201 aaaattttgc cacttattta ggaaaaacgt aagtattaaa aaaacacaaa caagcgttga
    22261 gaacttaatt gtcatgatag tcattccata gattcgctgc ccaatccatt gcaattccac
    22321 aaatgattcc tagcataaaa ccaagcaaaa aacgtccgaa tgcaagtcca atcaatacac
    22381 ctaagatcat tcccagtcct gaaaattctc cgtcatattt ctccatctta ataagtcctc
    22441 ccatttttga gcttgacaac tatgtaaaac ctcgctatca ttagtgtgtt attattacgc
    22501 agatgtgttg gaattggcag acaagcatga ttgagggtca tgtgagcgtt agctcgtgtg
    22561 ggttcgagtc ccatcatctg cactacaact cgtgaagtta attttgcttc acgagttttt
    22621 ttgtttgaac gtttctttat gccattatat acatatcact gtatccttta caaaaagaac
    22681 gtacaatcat gtaaacagcc tgaggtgagt atatgaaggt cggaaacaag ctactcgtag
    22741 aaacttgcaa gtgttaaaac aattaatcga aaaacgagta aaatccctca cctaatacta
    22801 aagcgaggga ttttttgtat attatctgtt tatcaccgtt attataaagg tacaaatacc
    22861 aagcacggtt aatccaaatt gcataaattc atttttttgt acactcatgc ttttcattag
    22921 ctctttaact gccattagta ctaagatggc gataacaaca taaagtatat agtacgtccg
    22981 cattccggaa gcccatactg tgggagaaaa tcccataatt aaccttgaac aaaatcccat
    23041 tatcatcaaa ataactaaaa aaatagaagt tttcttattg tcaaaactta aataaattcc
    23101 aacaattaaa catagtaata atgccaagac tactaaagta gctatccaag tacctggata
    23161 aaaaagtgac agtttcgttc ctaactctgt aaagagatta ttcaggttac tactactcca
    23221 aatcattgcc cttttatttc cattgacgta agtaaagctt tgacccattg tattaccaaa
    23281 ataaataatc aggtttaaga aaaacggtaa tgcagtcaat atacgcacat aatagttttg
    23341 acatttccta tatgttagaa ggaaaattaa gaaaaatagc aataaaaata agatattcat
    23401 gtctaaaa
//
LOCUS       DAZUKD010000040        23177 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000040 DAZUKD010000000
VERSION     DAZUKD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.041"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..446
                     /locus_tag="ACG8N9_001296"
     CDS             234..446
                     /locus_tag="ACG8N9_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896907.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            453..677
                     /locus_tag="ACG8N9_001297"
     CDS             453..677
                     /locus_tag="ACG8N9_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896908.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            683..1237
                     /locus_tag="ACG8N9_001298"
     CDS             683..1237
                     /locus_tag="ACG8N9_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4896909.1"
                     /translation="MKGGGLNVVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSAR
                     VIGKENLTKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLE
                     KNKVIKHMQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQIN
                     SERSTEEKLDELLEKISGELDGTS"
     gene            1224..2468
                     /locus_tag="ACG8N9_001299"
     CDS             1224..2468
                     /locus_tag="ACG8N9_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFS4896910.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            2481..3980
                     /locus_tag="ACG8N9_001300"
     CDS             2481..3980
                     /locus_tag="ACG8N9_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4896911.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            3985..4911
                     /locus_tag="ACG8N9_001301"
     CDS             3985..4911
                     /locus_tag="ACG8N9_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS4896912.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5038..5640
                     /locus_tag="ACG8N9_001302"
     CDS             5038..5640
                     /locus_tag="ACG8N9_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS4896913.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            5653..6576
                     /locus_tag="ACG8N9_001303"
     CDS             5653..6576
                     /locus_tag="ACG8N9_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4896914.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            6594..6857
                     /locus_tag="ACG8N9_001304"
     CDS             6594..6857
                     /locus_tag="ACG8N9_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896915.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            6869..7198
                     /locus_tag="ACG8N9_001305"
     CDS             6869..7198
                     /locus_tag="ACG8N9_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFS4896916.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            7195..7521
                     /locus_tag="ACG8N9_001306"
     CDS             7195..7521
                     /locus_tag="ACG8N9_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896917.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            7505..7843
                     /locus_tag="ACG8N9_001307"
     CDS             7505..7843
                     /locus_tag="ACG8N9_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4896918.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            7843..8235
                     /locus_tag="ACG8N9_001308"
     CDS             7843..8235
                     /locus_tag="ACG8N9_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFS4896919.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            8246..8758
                     /locus_tag="ACG8N9_001309"
     CDS             8246..8758
                     /locus_tag="ACG8N9_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFS4896920.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            8804..9172
                     /locus_tag="ACG8N9_001310"
     CDS             8804..9172
                     /locus_tag="ACG8N9_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFS4896921.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            9226..9513
                     /locus_tag="ACG8N9_001311"
     CDS             9226..9513
                     /locus_tag="ACG8N9_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896922.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            9530..12649
                     /locus_tag="ACG8N9_001312"
     CDS             9530..12649
                     /locus_tag="ACG8N9_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4896923.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            12661..13449
                     /locus_tag="ACG8N9_001313"
     CDS             12661..13449
                     /locus_tag="ACG8N9_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFS4896924.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            13446..16277
                     /locus_tag="ACG8N9_001314"
     CDS             13446..16277
                     /locus_tag="ACG8N9_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4896925.1"
                     /translation="MSETVYFFDHLQKLIRRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            16252..18009
                     /locus_tag="ACG8N9_001315"
     CDS             16252..18009
                     /locus_tag="ACG8N9_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4896926.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            18026..18808
                     /locus_tag="ACG8N9_001316"
     CDS             18026..18808
                     /locus_tag="ACG8N9_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896927.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            18808..20994
                     /locus_tag="ACG8N9_001317"
     CDS             18808..20994
                     /locus_tag="ACG8N9_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4896928.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            20997..21443
                     /locus_tag="ACG8N9_001318"
     CDS             20997..21443
                     /locus_tag="ACG8N9_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896929.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            21445..21582
                     /locus_tag="ACG8N9_001319"
     CDS             21445..21582
                     /locus_tag="ACG8N9_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4896930.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            21619..21912
                     /locus_tag="ACG8N9_001320"
     CDS             21619..21912
                     /locus_tag="ACG8N9_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896931.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            21935..22132
                     /locus_tag="ACG8N9_001321"
     CDS             21935..22132
                     /locus_tag="ACG8N9_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4896932.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22143..23162
                     /locus_tag="ACG8N9_001322"
     CDS             22143..23162
                     /locus_tag="ACG8N9_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4896933.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
BASE COUNT         8130 a         3768 c         5043 g         6236 t
ORIGIN      
        1 taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaactg
       61 agggttggaa atgggcgctc aaagtacacg agcaaggcga ggtcgatagt aatcgatgga
      121 atcggtgtag gttgcttgat agaactataa ctgcatctcg tttatgaggt gtagttttta
      181 catattagat cactcatcga gtggtctttt tattttggaa ggggagtaaa aaaatgaacg
      241 aaaaccaatt aagagagttg tttaaaacga atgaagcaaa caaaacaatg gaggcgacat
      301 tctacgaaac tcaaaaaagc ttagcgttaa tcgcaaaaca agctaagtat ttctacgatc
      361 agcttattct gcaaggattt aatgacgcac aggctatgga atttatgatg cgaacctttt
      421 ctgccagtaa ccaacagaaa gagtgatacg taatgaggaa ctactggtat gtatcattat
      481 caaataaata tccgcaacca aacgcagatg atccaattag agttgtccag tcagtccaga
      541 ttaaaaagaa gtactccatc attgaaatga ccagagaagc tacgccaaaa gagattgata
      601 agtacaatct tcgttactgc ggccatggat attttagtga gcagaacata cagacaaata
      661 ttgagagata ttgttaataa aattgaaagg tggtggcttg aatgtggtaa atttgacacc
      721 aaaacaaaaa gcttttgcgg atgaatatat aaaaaatggt ggtaatgcta ctcaagccgc
      781 cattaaagca ggttatagca aacgatcagc aagagtaata ggtaaggaaa acctaactaa
      841 acctaacata atacagtata ttaatgaacg gctaaatcct atcgaaaaga agcgcaaatt
      901 aagtgccgag gacgctttaa atgaattgat agatatttgg caaggagaag tacaaataag
      961 cgtgagcaag caaatagacc gcttggagaa aaacaaggtt attaaacata tgcaatatga
     1021 atatacacca gatttagaaa gcaaattgaa agccttggat ttgtatttga agtataaatc
     1081 gctgttatca caaacgcaat tagaaaaagc tcaaacagaa ataaaattaa tgcaagcaaa
     1141 attagaacaa ttacagataa actcagagcg ttctaccgaa gaaaaacttg atgagttgtt
     1201 agaaaagatt agtggtgaat tagatggcac tagttgatat ttataaccca aagcaaatcg
     1261 acgtgttaaa taaaaccatt aagaatgatt ggtttattac tttattacat ggagcaaaac
     1321 gttctggaaa aacaaaaata aacaatgatt tgttcttgtt tgaattgcga cgtgtgcgaa
     1381 agattgccga tgaagaagac attaaggaac caatgtatat cctagcagga gtttcaagtg
     1441 caacaatcca aaagaacatc ttacaggaac tatacaacat gtacagcata gaacctaaat
     1501 tcgataaaca tggaaacttt aagctatttg gcgttaaggt cgtacaagct tatacaggaa
     1561 acattggcgg tgttggcgca attcgtggta tgacagcata tggcgcttat atcaatgagg
     1621 catcgctagc tagacaagaa gtatttgctg aaatcgtttc acgttgttca ggaacaggag
     1681 cgagaatact agctgataca aaccctgaca acccggaaca ctggctaaag aaagagtata
     1741 tcgacaattc aagcaaaaat attcaatcgt tccactttga attagatgat aatacatttt
     1801 tatctgaacg atatcgtaac aatattaaag aatcaacacc aagcggcatg ttttatgatc
     1861 gtgatatcaa aggtttatgg gtttctgcag aaggtgtcgt ttatcgtgat tttgatgcta
     1921 gcaagcacta catccagtca aaagacttac cacctttgag caacttttat tgtggtgttg
     1981 actggggata tgaacactgg ggttcaatcg tagttatagg tgaaacggat gacggaacag
     2041 cttatttaat cgaagaacat gctactcaat ttgaagagat tgattattgg gtagatgtgg
     2101 caaaaggcat tcaagagcgt tacggcttac gagtaccttt ttattgtgac tctgcgagac
     2161 cggagcatgt ggctaggttt gtaagagaaa agattaatgc taaaaatgct cataaagcac
     2221 ggttatctgg agttgaggaa gtcgccaaga gatttaaaca agataaattg tttatctgtc
     2281 aagatagagt gatgaaattt cgggatgaaa tttatcaata catttgggac aagaaaaaag
     2341 gcgagccaat aaaagaatat gatgatgtgc tagactccgt tcgatatgcg atatatactc
     2401 atgagcttct taagaaacca aaagttaatg tcaacgaaaa gattaaacgt attaaacgca
     2461 tgttttaagg agtgtgaaaa atggataagg taaacgagtt tgaatacgga gctgatatac
     2521 attattctaa tgacgtgaac acaaattatg taaagtttag cgtagactca aatcttcact
     2581 atagatttag ctcagcagaa gatttactta acgatttaga tactttagca gcaatgataa
     2641 aacatcatca tgaatatcag gtaaaaaggc taagtgtatt agatgattat tataaagcta
     2701 gaaatacaaa tatcatggat aaccgtaggc gcagagaaaa agaaaaagcg gatcaccgat
     2761 cagcacataa ctttggaaaa gttctttgta cgtttgatgt tgggtacaac acaggcaatc
     2821 ctataaaagt gcaaatcgag gacacaaatc aacaaaaaga aatcgaagag tttaatacta
     2881 ataatgacat agatgggtta aatgctgaac tctggcttga tatggataag tatgggagag
     2941 cctatgagat tatctatcga gattcagatg atacagatta tgttgatttg gctaatgtat
     3001 ttgaaacgtt tgttgtatat gatactacag taaaacgaga gcctattttg gctgtacggt
     3061 atcctaagac aagattcaac aaggatgctg ataaacagta cattcaacca atcgtataca
     3121 caaaagaaaa aagtatcact tatgatgaga cgacactaac agcaattgag ttaaagaatc
     3181 cccaggatga accgcatgaa tataaagagg tacctattac agagtattct cctaatcgtt
     3241 ttcggatggg cttgtatgaa gatgtactat ctttgattga tctatacgat gcagggcagt
     3301 ctgataccgc caattatatg actgatctaa acgatgctct tctagttatt agtggtgata
     3361 ttgaagcagc aggactatcc acagaggacg ccatcaagca gaaagaagcg aatatgcttt
     3421 tgcttgagtc tggaactgat gtgaacggta ataaaacaag tgtgactgca ggatatattt
     3481 acaaacaata tgatgtgaac ggtgtagaag catacaaata cagagtacgc aaggatattc
     3541 acgaaatatc catggttcct gatcttactg atgacaattt ttccggagtg caatcgggag
     3601 aagcaatgaa atataaatta tttggatttg aacaaatgac ggcaacaaag caaaggctat
     3661 tcaaaaaagg ccttatgcgg cgttatcgtc ttttatttag cctaaaatca agtatttctg
     3721 aaatggataa ctccgatttg aaaggcttac gtgtaatatt tacgcctaat ctacctaaag
     3781 ccattctgga agagttgaaa tctttggttg atgctggagc tgaactcagt caagagacga
     3841 tcttaggact cgcttctttt gttccagatg tacaggcaga gttgaaacga gtaaataaag
     3901 aaacgcaaaa gcagattggc atttttgatt cagatggtga agaagtaatt aacaacaaaa
     3961 aagatgaaac aggggggtga ttaaatgaac tcccaagaat attggatcaa acgggaaaag
     4021 gaatggcaaa agcaacaaat taaagatgat aaaaagcgca tggcagaaat taaaagtcgc
     4081 atgcaatacg cacaagatgc gatacaaaaa gaaatagacg cgcagtggga cagtttctcc
     4141 aatggccaga aaatcactcg tagcgaagcg atgaagcgtg ctagtgaaat ggatgtcaaa
     4201 gcattcgctc gcaaagcaaa gaagtatgtc aaagagaaag atttttctcc tacagcaaac
     4261 caagaattaa agctatacaa tcttacgatg cgtgtaaata gattagagct cttaaaagct
     4321 aatatcgggc ttgaattgat ttcactgttt aatgaattgg ataagtactt ttcgaatgaa
     4381 ttaacaaaag ctggtttagc tgaattgaag agacaagccg gtattttaga aatgactatt
     4441 gcttcaagtg gatatgcaaa gctgatagaa ctagtaataa acagctcctt tttgagtgat
     4501 gacgtgtctt ttagtgatcg cttatggatg tatcaatctg aattgaaatc agaattagat
     4561 aggttgttag tcagaagtat aacgatgggg aaaaatccca agcaacttgc atctaaattg
     4621 gcagaatatt taacagctga aggacgagaa aacactaagt tcaacactca acgtttgatg
     4681 gtgactgaaa cgactagagt tcaggtaggg attcaagaac gaagttatag agatgcaggc
     4741 attacccagt acatctatat agcagaacca acggcgtgca aactatgtat accgttaaat
     4801 aatcaagttt ttgatgttgc cgatatgcag ccaggaagta acgctcctaa tatgcatcca
     4861 ttttgtcgat gcagtacagc accttatata gaacgaatat caagccgtta acacaaatta
     4921 acggcttttt attgtgcctt cttacagctt acaggcgtta aagagaaagc tattttcggc
     4981 tgaccggcgt aactggtcaa atttatcggg tagcggcgta accgtggagg attaatcatg
     5041 aaaaaacgtt tatttatgcc aatgaactta caattttttt ctgaaccagg agatggtgga
     5101 tctggtgatg agggacaaca aggaaaccta ccagctggct cacaagagac accgcccgaa
     5161 gcaaaagaag aaaacaatac tggcaaaaca ttttctcgtg atgaagtagc gaaaatgatc
     5221 gctgctgaga cgaataaagc aaaagcagcg tgggaaaaag aactagcagc aaaaaaagaa
     5281 gaagctaaaa agctggcaaa aatgaatgcg gaagaaaaac tacagcatga gttggagcaa
     5341 aaagaagctg aaatcgctga attaaagcgt ggacaggcac tatctgaaat gacgaaagaa
     5401 gcttctaaaa tgctgacaga tgcaaattta ccacacgatg atgatttact tggtctgatt
     5461 gtttctgatg atgcagatgc cacaaaacaa gctgtagcag tcatcactaa ctttgcttct
     5521 ttgattaaga gagaaaacgc aagacaaaca ccaccaaatg aaggtggaca atttacagca
     5581 tcgaaaaata ctaaagaaac agtggctaaa ctagctgcta aaaatcgaat tatcaaatag
     5641 gaggaaaact taatgaaaaa gaaacaactt ttaccaatga acttgcaaat gtttgctcaa
     5701 acatgggatc cagataatgt cttggtatat gaaacgaaag agggaaaaat tcctgataaa
     5761 tataatacgc tcattttgag tgaagttatg gaaaattcta agatcatgca gttagcaaaa
     5821 tacgaagaaa tgactgacaa agaaaagaaa tttgaatact ttgcagaagg accaggcgca
     5881 tactgggtgg gtgaaggtga aaaaattaaa acgtctaaac ctaaatggat gcaagctacg
     5941 atgactgcaa aaaaactcgg tgtcattctt ccggtttctc gtgaatattt aaattataaa
     6001 ttatcagatt tctttgagga gatgcagcca aaaattgctg aagctttcta taaaaaattt
     6061 gatgcagctg ccttattaaa taaagaaaac ccatttcctc agtcactaga cggatcagtt
     6121 attagtgcgg ggaatgtggt tgaaggcgga ttgacttatg ataatatcct agccttagaa
     6181 gacaagttag cagaaaatga attcgaacct aatgcgttta tttcaaaccg aaaaaatcgt
     6241 acagaattac gttctgcagc tcaaacagtc gggtcaaatg ttgagtttat ttatgatcgc
     6301 tctactaata caattgacgg attaccagta gtagacctta agtctttaga taaaggaaca
     6361 ctttacgctg gagactttaa ttacatgttt tatgggatcc catataatat ttcatttaag
     6421 atttctgaag aagcccaatt gtctacttta actaatgaag atggaacccc agttaacttg
     6481 tttgagcaag aactgattgc tttgcgtgca acaatggatg ttggatttat gattgtaaaa
     6541 gatgaagcat ttgggaagat ttccccaaaa gcgtaacgcc tgctaccggt attgtgccaa
     6601 atcaaaagac atggaccggt aaagtaggcg atactaaaac atttactatt tcagctgtgc
     6661 ctgcagatgc tagcgatgca gctgctgttg ttgcagctac tacagcaact tcaagtgatg
     6721 gagctatcgc aacagtgacc aaaaatgaaa atggtggttt tgatggaacg attgcagcag
     6781 aagggtcagc aacattcaca tttacttctg gagaattcac tacttcaatc aatgtgacag
     6841 gtcaacctgc tagttaggaa gtaaaaatat gatgattgca gatgatatta aaaaacttct
     6901 taaaggaaca atagatgaaa agcttgaagt tattgagcga agaacgaatg agcgtatgaa
     6961 aaccttgtta aatacgcaag aagttcctaa agaatttgaa acagttgtat atgaagtatc
     7021 gttgaaaaga ttcaatagaa ttggtcaaga aggtatgcag tcatattctc aagaaggttt
     7081 atctatggct tttcctgact cggatttttc agagtatcaa aatgagattg atgaatttaa
     7141 gcgtaaagat caggaagagt tgtacaagcc aaagcgaggg aggtttaaat ttgtatgaga
     7201 tttacagatg aaattatatt tgttaaacgt tcatctgact ctaaatatga tccagatctc
     7261 ggtgagtggg ttgaaggcaa accagaaaga acaagaacag aggcaaacgt gacagatatt
     7321 ggcactgata gaagtgtgac tatttttggt agtgtggaag aaggggcgaa ggtcattagg
     7381 acgcagcctc ttttttctat ccctacattt gactatatcg agattgaagg aaagacttgg
     7441 caacaaaaaa cagctagaaa tccagcatat agaaatagtt taattgtgca agaggtggtt
     7501 cttgatgaag gcacaacttg aatataaagg aatcgatcag ctgatgcgac atctgaaaaa
     7561 agcagcaacg cttaatgacg ttcaaaaagt cgtgaaaagt aatactgctg aaatgactga
     7621 acgaatgcaa aaaggtgcgc cagtggatac aggttactta cgaagatcaa taaacatgaa
     7681 tcttttagaa gctggtttaa ctggtattgt aggaccgaca gcagattatg caccttatgt
     7741 agaatatggc actcgattta tgtcggcaca gccttatgtt agaccagcgt ttaattacca
     7801 aaaagtcaaa tttatggctg aaatgaaagc cttggtgaaa tgatgattaa gacaagagat
     7861 caatcaattt ttgatgaact ttttaaaata tcccaaaaca aacttggata taaaacatac
     7921 gattacaaaa ctttagagga tgttggttat ccttttgtgg aatttgagaa cactcagaca
     7981 atccatgaag taaataaaac tgacattaaa gggtctgtga ttgtggtttt atccgtttgg
     8041 ggattacaga agaaacgaaa gcagttgtca gatatggcat ctgctctttt taatgaagct
     8101 agattgatag aagccacaga aggctattat tgggctttaa attatcaagc aagtggaatt
     8161 caagtgatgg acgacacaac aacgaataca ccgctaaaac gggcggttgt cacacttgaa
     8221 tttagaatta gataggagga agaacatgga agcattaaaa ggtattgatg tcattttgct
     8281 ttatcgctta ttgaaaaaag aaactcagga agccgcttgg aaaatggcat ttcaaacaga
     8341 acacgaaaat ggtttatcaa gagattcaga ctctacagtg acaaaagatg gaaacgttca
     8401 aagtttaagc ccggttgaat atgatttttc ggctacttca atagttgcta aaggagattc
     8461 tcatgtagat gaaatgaaac aagctttatt aaatggggat atcattgaaa tttgggaaat
     8521 taataaagca gaacagggaa cagatgataa tgcaaataag tacaaagcta cttattacca
     8581 agcatatgtg tctgaattta ctccatcggc tgctgcagag gataacgttg aattaagttt
     8641 atcatttgca gtaaatggtg ttggtcaaga tggttatgca accttgacag aagatcaagc
     8701 cgatgttgtt caatatgcat tcaaagatac cgtgaaagca acttcgacag gagcataaga
     8761 gggcttagat gctctctttt ttattttagg aggatgaaaa acattgaaat taaaaattaa
     8821 aggtaaagaa tattcgttta aatttggcac taaatttgta cgtgaattag ataaagtgat
     8881 gcctttcgtc gatggaaata tggaatttgg aatgggactc tccgcaaaag tcttaccaga
     8941 gctacgttct tataacgtta acacgttatc acgagttctg gaaatagcaa atagaactga
     9001 agatgaatct attacattag atgaattgga tgattacatc gatgaagtta aagacattga
     9061 aaaattgttt gatgaagtcc taaaagaatt ggcggagtcg aacgcgggaa agttagcggt
     9121 ccgaaacctg aatcagaaat tgaaagaagc ggaaaaacaa caagtggaat agattctgca
     9181 ctggcatacg aacaaattct tatcaattct tttcgatatt tgggaatgac caatatctca
     9241 gatatcgaaa gaatgacgtt atatgaatac aacattcgta tgactgcagc ccagttatct
     9301 tggcttgaca aagaaaagtt gattcacgaa ttagcgtggg caaatcagca agtccaagcg
     9361 gagaaaaaag taggcaaaaa gacagttcct gtatatcgat cctttgaaga attcttcaat
     9421 tatcaaaaaa tcgaagattc aatcatggga gtttccgaac tttcaaaaca agataaaaaa
     9481 ttccaaagct tactaactaa agctaactct tgaggaaagg aggagaatca tggaacaatt
     9541 ttctgttgaa gccttattaa aagccacaga tagtggattt gtaaagactt ttaaagatgc
     9601 acaagatgct gttaaaactt ttgaaaagaa ttcaaatagt atgacaaccg ctgttggtaa
     9661 agtgatgcaa ggtactggtg ccgcaatgac aaagtatatt accactcctc ttataggagt
     9721 aggcgtagca gctgctaaag ttggtggcga cttcgaagca caaatgagtc gtgtaaaagc
     9781 tatatcagga gcaaccggcg acacattcga acagatgaaa cagcaagcga ttgatctagg
     9841 agcaaaaact gcttttagcg caaaagaatc agctgctgga atggaaaact tagcttctgc
     9901 tggatttagc gcacaagaaa tcatgaaagc aatgccgggt cttttagact tagcagctgt
     9961 atctggaggg gatgtggctc tagcttctga aaatactgct actgctttga gaggatttgg
    10021 tttagaagca agtgaagcag gacatgtcgc tgatgtattt gctcgtgctg ctgcggatac
    10081 caatgctgaa gttggagaca tgggagaagc attgaagtat gttgctcctg tagctaattc
    10141 aatggggatt tctttggaag aaactgcagc agctattggt attatgagtg acgcaggcat
    10201 taagggttct caagcaggta caacgttgcg aggagcattg tctaggttag caaggccaac
    10261 aaaggctatg caagatacaa tggataattt aggtgtttcg ttttatgatg ctgacggtaa
    10321 aatgaaacct ttaaaaactc aagtagaatt acttaaaaaa gcttttgaag gcctgacgcc
    10381 tgaacaacaa caaaatgctt tagtaacact atatgggcaa gaatcattat cagggatgat
    10441 ggctttgatt gataaaggac ctgattcatt gggcaaatta acaaaatctc tgaaagattc
    10501 tgatggtgca gctgacgata tggctcggac catgcaagat aatatgaatt cttccatcga
    10561 gcaaatgttt ggagcttttg agtcagcagc tattgtaatt caaaagattc tagcaccatc
    10621 catcaaaaaa gtagcagatg ccatatccgg cttagtagaa aaatttgtaa gtgctccaga
    10681 atcaactcaa aaattagtag tggccatagg agcaattgtc gctgctatag gaccgttaat
    10741 ttttatgatt ggttcagtaa ttatatggat caatagggtg aaagtagctt ttaaagcttt
    10801 aagtgaaagt tcaaaattgt ttagtggatt aagtaaagca atgggtcttc ttacaaatcc
    10861 ggtttttctg gttatagctg cggtagcact actcgttgta ggtttcatct atctttggaa
    10921 tacgagtgaa gattttagaa acttttggat tggcttatgg gagggaatca agtctgctgt
    10981 aagctcggca gtagaatgga ttcagaatgc atggaaatct acaggagaat ggtttaacaa
    11041 tttatggaag tccattaaag aaggcgcaga caatgtttgg actacagttc aagaagcccc
    11101 tgggaaagcg gcagattgga ttaaaaacaa atggactgga acaaaagagt tcttttcaaa
    11161 tttatggtca agtattgcaa actctgcttc agagatttgg aatagtttaa aagaaggtgt
    11221 catatcagtt attgatgatt tagtttcaag tgctggtgaa aaatgggaag ggtttaaaaa
    11281 tactatatct actgcatgga aaacaattac aagtaaaatc aaatctggtt ttgattttat
    11341 actaaaatat attggtccat ttgtaagtag cttttcagat gtgttctcta atatagtgaa
    11401 agcaataaca agtatatttg ctgaggttaa aaacataata gtaaatgctt gggaaatcat
    11461 taagtcttta atagctgcgc cactactgtt tattatagat ttaattactg gtgacttcga
    11521 acaaatgaaa gaggatttag atctaatctg gaacacactt gtccaatcag tggtaaatat
    11581 ttggacatct gtaaaaaata tatttacgga atatatcggt gcaatagtaa atagtgccgt
    11641 tagtttatgg actggattca tacaaagtat ttctaatatt tggaatgaag tagtttatca
    11701 agcgactatg atttggattg atttgaaact attttttact aatttatgga ttgatattaa
    11761 atacagtgca attcaaatgt ggataaatct aaaattctcc ataattcaaa cttggattga
    11821 tacaaaatat ggtgcaattg aactttggaa taatctaaaa caatggtttt tccaaacggt
    11881 taataatatc gtgcaaactc ttataaaaag ttggaacagc ttaaagcaag gaacgataga
    11941 tttattcaac aatacagttc aaggtgctaa agacatttgg acttcattca aatcttggat
    12001 tggtgattta attactggaa ccaaagataa catcattcag ggatgggaaa acctaaaaca
    12061 aggtactata gatactttca acaatttagt aaatggtgct caagaggcat gggataattt
    12121 agtaaatgct gttagtgaca cggttgatag agtaactggc tggtttgata acttgaaaaa
    12181 tatcgattta ctagcagccg gaaaagctat catggatagt tttctagaag ggttacaaaa
    12241 tgcatggaaa tctgtgcaag attttgttgg aggtattggt gattggattc gtgaacacaa
    12301 aggacctatc caatacgata gaaagctatt gattccagct ggtcaggcta ttatgaacgg
    12361 tctgaataaa ggtctgacag gaggattcaa tgacgtacag aatactgttg gaagtatggc
    12421 agactttatc gcggaacttt tcaatgcaaa ttctgatgta gatatagctg caaatttgaa
    12481 aaatgcaaat aaaaacattg gtgcacaagt tgaacataaa ataaatatgg gtggctctac
    12541 taaaccagct gtatttaaaa tcaatcttgg aagacaatcg tttagattgt ttatggacga
    12601 tatttcacaa gctatgggcg aaggtgcaga tattaatctg gaattttagg agggaatatt
    12661 ttggatcaac gagaaaataa aatgtactca ttcaaagata caaccattaa tctcactagt
    12721 tctaaacgat tccttccgac gtctgccatg atgtacgatg gaatgtattt agaagatttg
    12781 attgatggtt atcaaacact tacggtggaa ggtagagaaa tgctttctgt agaagttgaa
    12841 cagcaagaga tacaaattgg ttcaatcatt acaaatcaga aaataccttc aagaacacta
    12901 aaaataacat ataagctgga agatagagat ccagaaaaac tacagtttaa attcaaagaa
    12961 ctgttgaatt atttataccg gaatgaagac gtggaaatta ggtttcatga tgaattagat
    13021 tattattact acggtcgcta tacatcaact gatactgttc caggagactc caactcgatt
    13081 atttcgagtt ttaatgtatt ctgtgcggat ccactaaagt atacgaaaga gtgtgttagt
    13141 gatggctata ttggaaatcc gatacagttt cctataacac caagaaaaat tgaagttact
    13201 ttatccatga ataattcaat caaaattaca aacggagaac aaaatatcac gataactgac
    13261 gcggcaataa aaacaggaga cgtgttggtt tttgattttt ccgatgagca ggtaactgta
    13321 aacggagaag attgtacttc tatgattgat ttagaaagtg attttgagaa cttttatctt
    13381 aagcaaggtc agaagataac tagcaataat gggaagctta aaatattcta taggggggcg
    13441 acaatttgag tgagacagtt tatttctttg atcacttgca aaaacttatt agaagaaaaa
    13501 atacaagaag tttgattgaa gtctcccaag aaaaagaaat tagttctgat aagagtgatc
    13561 taatgaaaga tactctttac gttacgacaa aatatgataa agaaatagag gatgcaagat
    13621 atatggcgat tcgtgaaaac gagtcgtctt tttcgttgta tcgaattact aaagttagcg
    13681 acccatttga aacattagag tttacagggt taggatttgc gacaaatgaa ttagatgctt
    13741 acatcatcaa agatattagg ccgagtgggc agcccttaaa aaatgttctt gatcgattga
    13801 ttgaatttac tgaaggaaat tggcgcgttg gtcacgtaga agcaatgtta ccaacagtaa
    13861 ctgcaacttt ttactatgtc tctgtaaaag aagcgttgaa agaattgcaa accttaggta
    13921 tggaatttgt ctttaggtgt tctttgaatt ctgatggaat aaaggataaa tggatcgaag
    13981 tatatgaaca aattggcgaa gaatcgaata cacgtttcgt atatggtagt aaagcattaa
    14041 cagttgtaag agagatagat agaagctcaa tctcaacttc aatgataggt cgtgggcgag
    14101 gcgaagaggt tggtgacggt tacggtagaa gaattgaatt cactgatgtt gaatggaaaa
    14161 agtcgaatgg tgatccttta gataagccta aaggccaaaa ttggcttgaa gatccggaat
    14221 caactcaaaa gtatgggata ccacaaaaag atggatcaat gagaaaacga gaaaccgtag
    14281 tagtgtttga tgatatagat gatccaacag atttacttaa aaatacttat tcaaccttaa
    14341 tcgattctgc tagaccgtta gtacaattca aagctgaagt cactggagga gatgtgatag
    14401 gaaatacagt gactattcac agatacgata aaggttatca ctataaaact cgtatttata
    14461 aaactacatt caatcggctt accggtcaaa cgaatatcga actaggggat aatttaacac
    14521 aagatgttag aaaacaaacg gcttctattg tcaataatat taatagttta gaatctagca
    14581 aaatgacatt ttacgaatcg acagagattg gaaaatatca agatgacatt atgcgaggtg
    14641 caggagataa tggcggttct atttattggg taaatggaat tgaagctggt gttagtgata
    14701 gtagagaaat ctatgaaact gtttatatgg atggacctaa cattcctaga tcacgctttt
    14761 ttatggtcca aaataactca gggatatctt tcaaacaatg taaaaaaggt gagtggcaaa
    14821 caattcaaga tgtacacaat ggtgatagca cgaccgcttg gacgttggat ggaactttca
    14881 atgctaattt tattcgcgca ggcattctgt ctggtatcct cgtgcaaggg gtagctttaa
    14941 aaacatttga cgataaagat ttccaattag tggcagaggg gggagaactt tcttttgaaa
    15001 aacaagtcaa gtctactgga ctagatgatg tacatggaga acgcttgggc tcaattgtat
    15061 ctacttatgg ttctaaaggg attaacggct ttgctgtctg gaaagagcca aactatattt
    15121 tttccattaa tgctggggac ggtggcgatc gagggaatcc tatttttcaa attccagcag
    15181 atgttactgc tgataagcgc aaatataatc tttatggtga cggcaatttt tcagaaggaa
    15241 atataaccat agatggacgg ctagatgtaa aagaactcta tgttaacggt acaaaaattg
    15301 acactaatgg aggaggcaat tccggaggga acgataacgg ttggaacgga caatacccac
    15361 cagaagtaac tactgatcgg gataaacgat attggcaaat ttgggcaacg gcaataggtg
    15421 ccggttttac acaacaagct gctgcagcct tacttggaaa tgcacaagga gaatcagatg
    15481 ctaatccaac cgctgatgag ggcaatggcg caccagggtt cggttatggt gtatggcaat
    15541 ggaccgattc ttctggcgca actagcggac gtgtctacat gatcaattta atgacaaagg
    15601 ctggcatcag tgatgatcca gacacaatca cggcacagtt taaattgttg atgtggcatg
    15661 caccaaatgg tcaatggatc gcaactagcg cttatcctta cacatggaca caattcatga
    15721 atctgaccga tatcaacaca gcagcacaag cattcgtggc taactttgaa cgtccacgtg
    15781 atccacatcc agaacggacg gcatggtcac aagaatggta tgacaagttc aaagatttgg
    15841 aaattcctgt atcaaaagga tatatgaagc cgattgcgga tccaatcaca gtgacgagcg
    15901 aatttggctg gcgcacttct ccaatcacag gagcacaaga atttcataac ggtattgacc
    15961 ttgtaaatgg aaatcctaat acacctattt ttgcttcagc ggatggcgaa gtgattgttg
    16021 caggtgatgc aaattacttt gactggtatg gaaattggac agtgattaaa cacgctgatg
    16081 gaatgtatac aggatacgcg catcaaagcc gagtagacgt ctcaaaaggt cagaaggtaa
    16141 ctgctggtca gcaaattgga ctgatgggga caacaggacc atccactgga gaacatcttc
    16201 attttcaatt tatggatgag ttttatccat cttcttcagg tcatttccac aatgcaagag
    16261 actacatcga tttctaaagg agggatagtc atggcagaaa cgcagcataa aatggtccta
    16321 tccaccaccg aaccaaacaa cggaataaat ttggttcgaa ttcggcaagg ggatgtttta
    16381 acgcaaaagt tcgttgttga agtggtggaa catggcaaac taaaaacatt cgagggccta
    16441 gtgccgtttt ttattaatac aacaaaattt ggcgaaaacc aacctgttga acaaaaagta
    16501 caagaataca gtccagcgca agcaaggctt gtttacacct taagtgagcc tgactggcaa
    16561 tggggtggtg aaaacaccgc acatttcagt ttccgatcac ttaatggtga tggaacttgg
    16621 agtgaacaat ttagcacaca ggattttacc tatcgagtca tttctggaat atctagaagc
    16681 cagttacgtg actctggcta tgtgtggaca tttgaggatt tgctaagaaa attcaaagat
    16741 tacatggatc agggcaaaaa tgactgggag cagtggttag aagataatcg tgaaatactg
    16801 gaaaatatcg atccaggtgg tatgatcatt aacattctga atgaagctaa aggcgactac
    16861 gaatcattag ctgatcgttt aaaccaaaaa taccaagtgc cagtcggcag ctcacaaatt
    16921 agagaaacaa cacgcttttt tgattacgac acgatgaaat acgttgacct agtgccgcgc
    16981 aatttgaata cggttgtcaa cagtgttaat aacagcaaat ttaacttttc tttcattaca
    17041 gacattcacg cagataatca caacttgcgt atagatggcg tcggttacaa agatgcttct
    17101 tatttaagac attggcgcgc aatccctcaa tttcaaaaat tagggaacaa aacagatgtg
    17161 atgatttacg gcggagacaa tatcgacggc ggacttggtt cactcggcag cgatataggc
    17221 attattgatg aatggagcgc gcgacattcc atgttaggca cgctcaaacg cttcactaac
    17281 gcagcggtag caggacaaga aaaaccggtt attatctgca aagggaatca cgacgcttgt
    17341 ttcgaccccg cgtggcgcaa gcgaaaggga atgttatgca acgctgactt cgaagagtat
    17401 tggaacggtt tgtacggtgg cgtgttgttc ccagataaaa acgcagcaat ttaccgtttc
    17461 gatacttgtg atttttatga aggcggcaca ggcgacaagt acgtggacgg ttacagcgat
    17521 acagcgccgg gagctttcag tgccaaacaa attaacgctt tcggagaatg gttagtgaac
    17581 gttccgagaa actatcatgt ggcgttagta ggacatacgc ctttaggtct cagcaagttt
    17641 cccgttcgca atgaaaacat gattagcacg ttgatagaag ggtttaaatc tggtagtccg
    17701 gtaactatcg attggtctaa attaggacaa ccgaatgacg gttcgttcgg cggattaaag
    17761 accttcgcaa tgaatacaaa aggcgccggc gttgtagtag gttatttctg cgggcattgg
    17821 cacgaacaag tcgaaggggc gtttggaaca gtaaaaatga ttctttgcga cgtcggcttc
    17881 tctcatacag ctagccaagt tgatacaccg gatgaattag cgttttataa aatagaagtt
    17941 gacacagcaa caagaaaagt gacaagcaaa ggcgtagggc gcgcgagaga cttcacttat
    18001 aactattaag gcggtgaaca attaaatgtt agattttcaa agcaaaccaa atatttttga
    18061 agaaatgagt tatgaagagg ctgtgaaatg gttattgcgt caagcggcta tccattacga
    18121 cggctcggat cacgacgcgc acgtcctagc tactgaaagc aacgcgggct ttgctacacc
    18181 ggaaacagtt atgcaagcgc gtgggcgttg gttacgcgat tataagctac cacaaaaata
    18241 tccgaacatt ttagacattc cgcccggcaa atacgcaacc aaagccggat ggggcgcaga
    18301 caaccctgac gggattgaag atgacagttt tgttgaaatg atggtattcg cggatcacga
    18361 ttcgagaaaa ttaatcgtcg cttttgctcg ttatagcggc gaaatttaca tcaaaatgac
    18421 acataacagc gaaccggtag agggttataa ctcgttaggc tggcggcgcg tttacactac
    18481 ttctgttctt tttgaaggcg aattaagaaa aggacaatca gtcaatctgc cagatgatac
    18541 tttccgttac caaacgcttc gtatccacta cacagacggt gacggagact ttgtagaaga
    18601 agtgaaacgt caaagatacg cgcgaattac aaaagcgaat ttatggaatg gagacgcggg
    18661 agtgacgttc atcgaatttg agctgacaat cgaagcgcga aagataacga tgtcaaacgg
    18721 cagagcttta gatatttcat ccggcaatgt ttccaatccc gcaatgagca acaacgtgaa
    18781 aataactaag attgaaggtg tgaaataatg gcgcatgtca tcaaaaaagg ctctatcaaa
    18841 gtacctacac agccgaaaga ctacgatttg caagcaacgg ggcttgtatt taaatcatac
    18901 gataatcaaa tagcgttaga gttcaacgtc gaacaacaga acggcacacc ggcggacttg
    18961 ctaggagcta acttgcgctt gttgatgttt atctatgatg aagtagatgg aatgatcacg
    19021 aaagagccaa tcccttttat cacgaaaaac ctcatcactg aaagcttctt gaatggacag
    19081 gtcgtatata tcttgccaga agcgatgaaa gcttataacg gtatggtgga agcttatgtt
    19141 tacatcgagt atccagacgg atcaacaagt gataacttag gcttcacctt ccgtatgaag
    19201 cgttcagcaa tcgacggact agcgcaagat aaagcagact actttattgc agactttcaa
    19261 caattacttg atggagtcaa acaagaagca acagatgctg ttaacgcagc attaacaaat
    19321 gtggattcta cgattgaaaa ggcaaaccaa caaataaatg aatttgtaga gggagccaca
    19381 caagcaattg atcaaactgt tgacgaggta acagagcaac tacaagctac tcaaactaag
    19441 attgataccg tttctcaaaa cgttacatcg gcacaaaaca atcttaaagc agttgaagac
    19501 aagatgaatc aaaccaatca gcaaatcggc gacctcggca agctgaaaaa gatgtacagt
    19561 aacagcatcg acttcgggaa ttatgattat agtgggaatc cgaatttaat gtccaaacta
    19621 aaatcgagcg attttaacgt tggttaccac gggtcactaa cttcggataa cgaaaagcta
    19681 cattttactt ctgatggtac aggaagcatt attatgttta cgcgtattaa tacacctcag
    19741 cttgctagtg ggaaaaccta tactctgagt gcgaaagttc gatttgatga aggaactaca
    19801 ggagctattg ataaattacg tttggtgtat cgtacatcac caggagaaaa gatattattg
    19861 gaagcaaata gtacaaatat tacaacagat gatgtaggga aagaaataac aatcaaaggt
    19921 acagctaacg ttaattatca aatcacaaat ttagatcgat tttatatgag tattagcttt
    19981 gttgacaggg ataaaataaa tggcggattt aagttgtacg acatcaaaat cgaagaaggc
    20041 tcaacagcca ccccatatca gccaaactta cttgatgacc cttactggct aggtaaagcg
    20101 cctttgggtg agaatattgc taataagtct gttacgtttc caatcaaatc tagcgcctac
    20161 agcctatacc aagctaacat ggaagaagaa tttgttttag gtcaaaccta tacaattacc
    20221 atgaaggcaa ccaaaccacc tatccaaacg ttcattgttt acaacgagga tagtggagat
    20281 tatagatatg gtaacttaga gccagtagaa gggttagttg atacatgggg tctgactttt
    20341 acaccgcaga aggttggagt taactacccc aaacggttaa ctattatcca gtatccccaa
    20401 tcaacaacag gtgcatgtca aattgactgg ctcaagatcg aaaaaggcga cacacgaacc
    20461 ccgaatatta gtcagtttaa atactttggt gaaggcttga aagacagtaa caatcccaat
    20521 gattacagtt gggatgtcac acctgaatat actgaaaaag gcttgaatga tgctgttaat
    20581 gtgtatgatc ctcagagagt tgaaggtttg aaaaactttg ccgatggtat tcaaatagca
    20641 ggagataaag tgatcagtga aaatgattgc actgtttata cattaactaa agacaacagt
    20701 caatcgttta tcgatgggta tgcaacattt attaaacacg gaaaagaagt tattgtaaat
    20761 ggtacagtaa agttcaaaaa agcttatgct tttggtgtac cacttgatga tgaagtccca
    20821 gatgaatttg tcgcaaaaat tgttcatggc atgcttacag gtccgtcagg aacaagtagt
    20881 gtatcaaaag cgatgtacgt gcgaaaagac ttaggaacaa ttattacgaa tagcgaattt
    20941 gctgcaaatg aatggtttac attccatggt aattattggg taggggagga ataaaaatga
    21001 aaaatatttg gaaatacgga cgaacaggtg gagagtatgc tggacaagtg cttgatgata
    21061 tggttatgac tgttccattt accgatgttc caccactcga aggaattcgt gctgatgggg
    21121 aaccactaac gattgctgat cagatgtttg atcctaaact gaaccaatgg attgttttag
    21181 tgaatgtact agatcacaac gatttaaaca atctcaaagc gatgtatgag tcgttagaaa
    21241 atgagaacgg cgatttaaaa cagctcaacg ccaaactcat gctaagcgat gtagcgatta
    21301 aacaggaaaa tactgcattg aaagaaaaag cggatggttt agcacaaatc aattcaaaaa
    21361 cgatgcttgc ttcgttccaa aatagcaaag acattgcaga aattaaagag caactaaatc
    21421 cagcttcaaa gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat
    21481 tggggctgtt ttactgacga tcaagttcga ctattcgttc cactatgcat tacagacgaa
    21541 gaagcagaaa aaatcattaa taaagataag agcgcatctt aagtgatgcg tttttattta
    21601 aggtaaagga gttgtcacat gattaattta ggagaatggg gaacaatcgc aggatcaatc
    21661 actgcgattg tttctttgat tttattagta ataagaccag tcactgcatc tttctcgaag
    21721 attactgaaa ctctttcaaa agtaagtcac aatttagatt tgctgactaa agatttagaa
    21781 tcgagcaaat cagatcgatt gatgattcat gaagaactaa agaaacacga tgaaagatta
    21841 gatacacata cagaaaaatt ggtagaacac acacaacaaa tcaaaacttt atttagagaa
    21901 agatctcggt aaaaaagaaa ggagttaaga agaaatgatt ttacccgata agtattatca
    21961 agtcattaaa tggacagttt taacagtatt gccagctgca tctgtattag ttgcaacact
    22021 aggcaaagcg tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc
    22081 aacattttta ggcgttatca ctggcgtgtc ggcttataat ttgaaaaaat aggaggaaac
    22141 aaatgaaaaa gaaaattact attactgcga tgagcctatt aacggctctt tttttattgc
    22201 caattaatgg gtttgcctat acgattaaca atgaatttaa tttaggccca aacgaaggta
    22261 gctcacaagt agcaaataat cagtacattt tactgcatga aacggctaat gaaacagcaa
    22321 caggacgcaa tgaagcgcag tatatgcaac gttcatggac tagcgcttat actgcttata
    22381 ttgtgggaga cggcggaatt gtttatcaag tcggtcaacc tggttatgta cagtacggtg
    22441 ctggttcgta tgctaatgca aatagtcctg tgcagattga gttacaacac acacatgata
    22501 aagcaacgtt tgagaaaaac tacaaggcat acgttgaatt agctagagat tcagcaatga
    22561 aatatggtat tccacttaca ctagacacac cgtataatca accagggatc aaatcgcatt
    22621 tatgggtaac acaaaatatt tggggcgatc atacagatcc ttacggttat ctttctgaaa
    22681 tgggcgtaag taaagaaaaa ttagcctatg atttggctca tggatttacc gatgaaaatc
    22741 cgacaacttc agatgataaa ccagtcattg atccaactcg agcaggtgct gcaaatccta
    22801 ccctgacaga tggaacaaat tacgcccaca ttgatcagtt cggagaaatc gaaaacgcaa
    22861 acttacatgt ggctggttgg cacatcgcta actataaata cgagtatatc ttcattatgg
    22921 attacaatac tgggaaagaa ttagctcgag taaaagctga tggaatatat agaccagatg
    22981 taaatcaagc ttataatact ttaggaaatg ttggctatca tgtatctttt aacatgcgca
    23041 acttccctaa taagaaagtc tatgtaatga tgcgggcaac gaatgatcca gaagggaaca
    23101 ctaaaggcgg agcacaagat tttcatgata aacgctggta tttaaatatt ccgcaacgat
    23161 aaaaatagcc cctcgtt
//
LOCUS       DAZUKD010000041        22507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000041 DAZUKD010000000
VERSION     DAZUKD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.042"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..2146)
                     /locus_tag="ACG8N9_001323"
     CDS             complement(254..2146)
                     /locus_tag="ACG8N9_001323"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896934.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2143..3870)
                     /locus_tag="ACG8N9_001324"
     CDS             complement(2143..3870)
                     /locus_tag="ACG8N9_001324"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4896935.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3886..4536)
                     /locus_tag="ACG8N9_001325"
     CDS             complement(3886..4536)
                     /locus_tag="ACG8N9_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4896936.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4740..5036
                     /locus_tag="ACG8N9_001326"
     CDS             4740..5036
                     /locus_tag="ACG8N9_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4896937.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5208..5828
                     /locus_tag="ACG8N9_001327"
     CDS             5208..5828
                     /locus_tag="ACG8N9_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4896938.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5920..7086)
                     /locus_tag="ACG8N9_001328"
     CDS             complement(5920..7086)
                     /locus_tag="ACG8N9_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4896939.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7098..7451)
                     /locus_tag="ACG8N9_001329"
     CDS             complement(7098..7451)
                     /locus_tag="ACG8N9_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4896940.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7579..8601
                     /locus_tag="ACG8N9_001330"
     CDS             7579..8601
                     /locus_tag="ACG8N9_001330"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4896941.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8620..8781
                     /locus_tag="ACG8N9_001331"
     CDS             8620..8781
                     /locus_tag="ACG8N9_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896942.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9040..9474)
                     /locus_tag="ACG8N9_001332"
     CDS             complement(9040..9474)
                     /locus_tag="ACG8N9_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896943.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9584..11443)
                     /locus_tag="ACG8N9_001333"
     CDS             complement(9584..11443)
                     /locus_tag="ACG8N9_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4896944.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11444..11584
                     /locus_tag="ACG8N9_001334"
     CDS             11444..11584
                     /locus_tag="ACG8N9_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4896945.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11561..12721)
                     /locus_tag="ACG8N9_001335"
     CDS             complement(11561..12721)
                     /locus_tag="ACG8N9_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4896946.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13028..14452)
                     /gene="araA"
                     /locus_tag="ACG8N9_001336"
     CDS             complement(13028..14452)
                     /gene="araA"
                     /locus_tag="ACG8N9_001336"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4896947.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14468..15166)
                     /locus_tag="ACG8N9_001337"
     CDS             complement(14468..15166)
                     /locus_tag="ACG8N9_001337"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4896948.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15123..16784)
                     /locus_tag="ACG8N9_001338"
     CDS             complement(15123..16784)
                     /locus_tag="ACG8N9_001338"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4896949.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17002..18402
                     /locus_tag="ACG8N9_001339"
     CDS             17002..18402
                     /locus_tag="ACG8N9_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4896950.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18654..19733)
                     /locus_tag="ACG8N9_001340"
     CDS             complement(18654..19733)
                     /locus_tag="ACG8N9_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4896951.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19819..20778)
                     /locus_tag="ACG8N9_001341"
     CDS             complement(19819..20778)
                     /locus_tag="ACG8N9_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4896952.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20790..22301)
                     /locus_tag="ACG8N9_001342"
     CDS             complement(20790..22301)
                     /locus_tag="ACG8N9_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4896953.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22323..>22507)
                     /locus_tag="ACG8N9_001343"
     CDS             complement(22323..>22507)
                     /locus_tag="ACG8N9_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896954.1"
                     /translation="LQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIFLFPPFIFIFAG
                     GVWKLVSIILTIGNER"
BASE COUNT         6404 a         4771 c         3981 g         7351 t
ORIGIN      
        1 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
       61 taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca aaacggaaaa
      121 atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
      181 gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa aaaaataata
      241 aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa agatctgcat
      301 agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat
      361 tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac
      421 ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct gttcgtgtat
      481 ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag aatgcccgcg
      541 caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg tttaatacag
      601 tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct ttagcagcct
      661 caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca tagatacttc
      721 ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg
      781 aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca tcgtagaacc
      841 gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca atagcgacca
      901 tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct ggtgtgtagc
      961 cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct ggaaggccag
     1021 atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg gctgaagcaa
     1081 ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga gagaactgtg
     1141 ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt gcaactttta
     1201 ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc atcaacggca
     1261 tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc tgattttctt
     1321 tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg gtatgaccac
     1381 catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga tctgcgaaaa
     1441 acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg atcatcaata
     1501 acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc ccaatcaatg
     1561 tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca
     1621 tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa taggaaatcg
     1681 gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt cgttgggaaa
     1741 cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga
     1801 ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt gttacttgtt
     1861 ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct gtggttgctt
     1921 tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct agtacaaata
     1981 cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt accgttcccc
     2041 agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca ccatgtcctg
     2101 gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta agtcctcctc
     2161 tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata gttcttcatg
     2221 tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg catccattac
     2281 tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg ttttcaatgc
     2341 ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat caaacacata
     2401 gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt gtccaccaga
     2461 gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct ctgaaacaaa
     2521 atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg catccggctt
     2581 accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt gaggaacaaa
     2641 gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc catttatttg
     2701 gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg tcgttttacc
     2761 tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc
     2821 ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga aggccaatgt
     2881 tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga taccgatttt
     2941 ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca ccatgataaa
     3001 aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca taaatgcaat
     3061 cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc
     3121 tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga cgatcgtatt
     3181 aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat tttcttcaaa
     3241 gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc gaaataccag
     3301 gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa agtacatgat
     3361 tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg tcaactgatg
     3421 atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat
     3481 acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg ttcttgtgat
     3541 caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg attttttata
     3601 gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa aaaatgtatt
     3661 tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca
     3721 aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg taagtgtcgg
     3781 caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa ttgctgaagt
     3841 caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt tatatactcc
     3901 gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc
     3961 ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag tattcatcac
     4021 taattggatc agtaattcca attcatgatc attttttact ttcagcttcg tccgatcgat
     4081 caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt gaggcgtttt
     4141 ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt tcataaacaa
     4201 gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt tagaaaaata
     4261 ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta acatatcttt
     4321 agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt tatcttcaaa
     4381 atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag caatcgaagc
     4441 ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc ttttttgttt
     4501 ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt ctttcaagat
     4561 gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt gcaagacatt
     4621 tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg aatccttcca
     4681 tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg atgagataaa
     4741 tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct gacagcatcg
     4801 ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac tcgctttcta
     4861 tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa ctttattata
     4921 ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact gctaatggtt
     4981 ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat
     5041 tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc
     5101 tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag ccttttcttt
     5161 ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt
     5221 tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt
     5281 cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt
     5341 atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg
     5401 ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg
     5461 aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc
     5521 taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt
     5581 tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc
     5641 aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt
     5701 taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc
     5761 ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat
     5821 cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag
     5881 catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa
     5941 cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac
     6001 tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt
     6061 cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag
     6121 tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac
     6181 tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa
     6241 aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca acgtgacagc
     6301 atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac
     6361 agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa
     6421 cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc
     6481 gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc
     6541 taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat
     6601 atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt
     6661 gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg
     6721 gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc
     6781 ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac
     6841 ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa
     6901 aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac
     6961 ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac
     7021 ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc
     7081 ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt
     7141 ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa
     7201 atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc gacataatcg
     7261 atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg
     7321 tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg
     7381 aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt
     7441 gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat
     7501 tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat
     7561 taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa
     7621 agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca
     7681 ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac
     7741 acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt
     7801 ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt
     7861 caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga
     7921 gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa
     7981 aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta
     8041 tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat
     8101 cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca
     8161 aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat
     8221 aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc
     8281 gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct
     8341 tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt
     8401 caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca
     8461 agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt
     8521 catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc
     8581 tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta
     8641 gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag
     8701 tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat
     8761 gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag
     8821 acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac
     8881 tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag
     8941 gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat tacatttata
     9001 ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt
     9061 agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga
     9121 tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc
     9181 acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt
     9241 tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt ttgttccttc
     9301 ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc
     9361 atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt
     9421 ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt
     9481 tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca
     9541 agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga
     9601 aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt
     9661 cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt
     9721 gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat
     9781 aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac
     9841 tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa
     9901 attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc
     9961 ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt
    10021 ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt
    10081 caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca tactttccat
    10141 cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct
    10201 tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta
    10261 tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat
    10321 aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg
    10381 attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg
    10441 tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa
    10501 attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt
    10561 gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc
    10621 gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta
    10681 cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt
    10741 ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac
    10801 cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt
    10861 tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca
    10921 gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca
    10981 ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca
    11041 ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca
    11101 aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta
    11161 atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa
    11221 tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc
    11281 aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa
    11341 ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa
    11401 tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt
    11461 tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga
    11521 acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct
    11581 ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga
    11641 gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt
    11701 tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta
    11761 atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc
    11821 gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg
    11881 cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag
    11941 gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt
    12001 tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc
    12061 ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca
    12121 atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa
    12181 ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca
    12241 agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac
    12301 caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt
    12361 ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta
    12421 tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta
    12481 cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt
    12541 tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca
    12601 ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt
    12661 ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca
    12721 taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc
    12781 atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata
    12841 aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga
    12901 tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag
    12961 ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa
    13021 cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt
    13081 gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg
    13141 aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat
    13201 taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg
    13261 taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac
    13321 gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg
    13381 cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct tggccactag
    13441 ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca
    13501 gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg
    13561 ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat
    13621 tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct
    13681 gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg
    13741 tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga
    13801 ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg
    13861 cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca
    13921 cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt
    13981 tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag
    14041 cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca
    14101 tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt
    14161 tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag
    14221 tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat
    14281 cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca
    14341 gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata
    14401 aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc
    14461 actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt
    14521 tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat
    14581 tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc
    14641 cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt
    14701 tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga
    14761 tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg
    14821 tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt
    14881 gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg
    14941 tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca
    15001 tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg
    15061 tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca
    15121 agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct
    15181 ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc
    15241 ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct
    15301 tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata
    15361 taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta
    15421 actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc
    15481 cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg
    15541 attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa
    15601 cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca
    15661 ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg
    15721 ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat
    15781 tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg
    15841 cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt
    15901 tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccgt
    15961 ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc
    16021 gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac
    16081 ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct
    16141 acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc
    16201 gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca
    16261 gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt
    16321 tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg
    16381 tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta
    16441 cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca
    16501 ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct
    16561 gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag
    16621 ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat
    16681 gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga
    16741 ccatctatga catctaacat actttgcgca tttacttcct tcataatgat ttaactccct
    16801 ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata
    16861 cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac acttgttttg
    16921 tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa
    16981 ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat
    17041 ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc
    17101 ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc
    17161 acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat
    17221 aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc
    17281 ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta
    17341 ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct
    17401 gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc
    17461 ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc ttggcgcgtg
    17521 atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca
    17581 gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac
    17641 tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa
    17701 caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta
    17761 gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt
    17821 tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg
    17881 tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca
    17941 gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttcattc
    18001 atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc
    18061 ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta
    18121 atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct
    18181 tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg
    18241 tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt
    18301 caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct
    18361 aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt
    18421 ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa
    18481 aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc
    18541 gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca
    18601 aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt
    18661 tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt
    18721 gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct
    18781 tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct
    18841 gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc
    18901 attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc
    18961 aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata
    19021 tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg
    19081 tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt
    19141 atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca
    19201 tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca
    19261 acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca
    19321 tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc
    19381 gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata
    19441 atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg
    19501 ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct
    19561 ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg
    19621 acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc
    19681 tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt
    19741 gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa
    19801 aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc
    19861 ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc
    19921 ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc
    19981 tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa
    20041 tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc
    20101 ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat
    20161 tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca
    20221 ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat
    20281 ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg cccacacata
    20341 atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt
    20401 ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag
    20461 tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa
    20521 gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca attcactcat
    20581 cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag
    20641 tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata
    20701 ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt
    20761 ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa
    20821 gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt
    20881 tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt
    20941 aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt
    21001 tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca
    21061 ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt
    21121 tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga
    21181 ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat
    21241 gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca
    21301 aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa
    21361 tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt
    21421 gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt
    21481 tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta
    21541 ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa
    21601 tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa
    21661 cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact
    21721 tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat
    21781 ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca
    21841 ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa
    21901 ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt
    21961 acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc
    22021 catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca
    22081 taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca
    22141 taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa
    22201 atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg
    22261 atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt
    22321 ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac gccaccagca
    22381 aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg
    22441 caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt
    22501 tgtaaaa
//
LOCUS       DAZUKD010000042        22114 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000042 DAZUKD010000000
VERSION     DAZUKD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.043"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..483)
                     /locus_tag="ACG8N9_001344"
     CDS             complement(40..483)
                     /locus_tag="ACG8N9_001344"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4896955.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(499..1659)
                     /locus_tag="ACG8N9_001345"
     CDS             complement(499..1659)
                     /locus_tag="ACG8N9_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4896956.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2166..3866
                     /locus_tag="ACG8N9_001346"
     CDS             2166..3866
                     /locus_tag="ACG8N9_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896957.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(3968..4801)
                     /locus_tag="ACG8N9_001347"
     CDS             complement(3968..4801)
                     /locus_tag="ACG8N9_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4896958.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5105..7270)
                     /locus_tag="ACG8N9_001348"
     CDS             complement(5105..7270)
                     /locus_tag="ACG8N9_001348"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4896959.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKNPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7530..9824
                     /locus_tag="ACG8N9_001349"
     CDS             7530..9824
                     /locus_tag="ACG8N9_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4896960.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9817..10482
                     /gene="pgmB"
                     /locus_tag="ACG8N9_001350"
     CDS             9817..10482
                     /gene="pgmB"
                     /locus_tag="ACG8N9_001350"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4896961.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10475..11482
                     /locus_tag="ACG8N9_001351"
     CDS             10475..11482
                     /locus_tag="ACG8N9_001351"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4896962.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11504..12523
                     /locus_tag="ACG8N9_001352"
     CDS             11504..12523
                     /locus_tag="ACG8N9_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4896963.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="ACG8N9_001353"
     CDS             complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="ACG8N9_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4896964.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14280..15701)
                     /gene="tyrP"
                     /locus_tag="ACG8N9_001354"
     CDS             complement(14280..15701)
                     /gene="tyrP"
                     /locus_tag="ACG8N9_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4896965.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="ACG8N9_001355"
     CDS             complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="ACG8N9_001355"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4896966.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18112..19368)
                     /gene="tyrS"
                     /locus_tag="ACG8N9_001356"
     CDS             complement(18112..19368)
                     /gene="tyrS"
                     /locus_tag="ACG8N9_001356"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4896967.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(19480..19685)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19948..21249
                     /locus_tag="ACG8N9_001357"
     CDS             19948..21249
                     /locus_tag="ACG8N9_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4896968.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21318..21917)
                     /locus_tag="ACG8N9_001358"
     CDS             complement(21318..21917)
                     /locus_tag="ACG8N9_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4896969.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         6686 a         4603 c         3939 g         6886 t
ORIGIN      
        1 aaaaattagg ctgggagcat tatttcgtta ttctgttcat caaaaatcag cgttttgatt
       61 ccatttcatc acaagttcct cctcatttac tgcttcatct ttttgctgtg tttgaattgt
      121 aaaaccttgt gaaagataga aatgaatagc tgattgattt ttttgataga ctgtcaaagt
      181 cagtgtttta ttggtttctt ttattttttt cattagttgc gtaccgattc tttgttgccg
      241 gtattccctt ttcacaaaga ttcctgcgat aaactgatca atcaccccag caaaaccaat
      301 aatttcttga tctatttctg ctaagtaaat cactgcatct ggtaaagctt tcctaacaga
      361 ttctacttga ttctcccaat aagttgatgg aatgaattca tgagaatcca agttagcatg
      421 cagccaaatc gatagaacag attccagttc tttggcagac aattttgttg ttgtgcggat
      481 cattttttct ctccttactt attcattgat tttaaaataa aaaccatttt tttgaagctc
      541 ttttacaagt tttcgcccct caaaaaagta tatcttctct tgttttactt ttctaatacc
      601 aaaagaaaca gcaactttag gatcatcagc tgaaacgatt cgataagttc caccaggcaa
      661 ttgatccagc ttgactttcc catgatggtc gctttgtact gaaaacggca caccatttct
      721 agtaagtgtt tttttcttat gtttcaaaat gaatgaacta ccatttaccg gtttccctga
      781 cgaatcacta ataataaatg aaaaatcaag cactttttta cttaatctat actgatagat
      841 tagtcgtccg ataaacacta acagtatgac tacagctatt atcgttcctg caagaataaa
      901 ccattgccgc atggttcttt cctgatttcc ttttccttct tcttttttta ccgattcgtt
      961 ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg ttgatcatat acggcgtaca
     1021 tgtaagcaag gtaactaagt cttctccttc tgaaatcgtt agaacagacg tgtcatctgg
     1081 cagtaccact tgtatatttt taacttgata agccaatttc tcccccaaaa cagtcaaaat
     1141 aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat aaccgtcgat taggtaaacc
     1201 gctgtgagca gagatcactg aatgcgtcga atttccacca agcgggaaag aggttccttg
     1261 caagacgcct gctccatttt caaggatcct accattgagc cgatcataaa gtgaaatatt
     1321 taattggatc ttaggtatat tgatacttcc gatcagccat tcctctaatt ggctggatgt
     1381 tgtactttca tttctgttct tttcattgaa aggatcagta ccaggattga atccttggtc
     1441 agttaatcgt tggtttttct cctccatttt ctttcgctgc acttcaattt cagctgcggt
     1501 ccgtttttgt acttgttcga tacgtttttg atcaataaaa ctatttaaag aatccacata
     1561 aaatggatat aaagcgatca gtaaccctac gacgatacat attaataaaa acaaacgaaa
     1621 caaaagtcga cgagactgtt tttgcttact ctgcttcaca tgtgtcctcc cttgcaagtt
     1681 tttttgttct ttcttttatt atatgggttc aggaaaaaaa tagaaaatga aagccgatga
     1741 ggttgtgaaa aagctgtcct gcatcaagta ataagtacag cccaaaaaaa tagctactca
     1801 tattttttgt tgggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
     1861 agaggttgtg aaaactgcgt tatgctttga gtaataagag cgctagtcag aaaatagctt
     1921 ctcatatttt ctaggctagt gagcttatta ccgagaaaca gctgattgaa caccgtttac
     1981 cgagaggtca tgaaaaagct actttgtttc gagtattaag aaaaattctg tttcaaaaca
     2041 aggagatgca acaaaactta tgtcgcatcc tctctcctta gaactttcac ttatgttttt
     2101 cttgtaaaat tcataaatat tttcatgaat tttctaattt cttgcctacc tctcgcatag
     2161 gatatatgat gaatttatct aaagtttgca aaggagttcg acaattgaaa aaatatggcc
     2221 catttttagc aatttttttc attgctttgg catttctcat gcctcattta ttcacacata
     2281 aactaatttt gggtgctgat gccccattcc attacaatcg gttctatgat gccgcgcaac
     2341 aaattaaata tggggatttc cattattttc cttcccttta tggattccag caaagtggca
     2401 ggatcgtgaa tgccgtttac ggcccatttt tcgcttattt acaaggattg atcgtgttgc
     2461 ttgcaggtag ctggtttcat tatcagattc tttcaaattt cattctttat cttatttcag
     2521 gattttctat gctgatttta ctgagatatg tcaatgttcg aaattgggta agtgtaccag
     2581 tagccgtttt attcatgagt acgtattcta ttcaatactg gtctatcaac caaggattca
     2641 ctagctgggg gacttgtttt tatcctttat gtatgatccc tattgctgat ttcgtccttc
     2701 ataaaaagtt ccctgttatc aaagtcgctg tctcagtggg acttatgaca cagatccatt
     2761 tactgtcttc gatcatgtta gctttgatgt atatcccttt ttatctttat tactttttta
     2821 accagactga aaaaggaaaa gcactgcttc aactgattaa gtcggtcttg ctttattttg
     2881 ttctgacagc aaatatttgg gcaggattag ctattgttta cagtgggaat aagattcttc
     2941 ctccctttgt taacagagaa atgagtcaaa aggcgataga tctggatggc agttattggc
     3001 tccattaccc taaggccttt cctatattga tattatcagg ttttttgctt tttattttct
     3061 ttcaacaaaa gaacacctta ttccaaaaaa tcgtgttgta cacaagtctt gttttctttt
     3121 ttctttctac tagcttgatt ccatggacga cattgatcga aaaaaatgtg ccttttatta
     3181 gtttgatcca gtttccattt cgatttttcg tgccatttac gctattgctt ctactttacc
     3241 ttgccctttc actgaaccag tggtctgaac aaaagtggtt ccgtctttta agtatatttg
     3301 gcattcttat tttttcagca cagacgatcc agaacttata ccagcatctg gaaaagtggg
     3361 aaaatgaaac atttgtcagt cgacatactt atctattcga tacgccggaa gaagcaagga
     3421 aaacattctt cagcaaagat atgtctacca gcttgttcgt tttccaaaaa accacacctg
     3481 actatctgcc aatctataag gagacaaaag acaataaata cgaccggtat aacgaagaaa
     3541 tactagaaaa cgaaacaact tttataaaaa cacatccagg cggtcttttg acaatcaagt
     3601 ggaaaaacac tggggacaaa actgttcata tccctgttat cgtctacgat cggactgtgc
     3661 tacagcaaaa tggaaaaaca ttgactgatt atgaagtaac agatatcggg accccaatcg
     3721 tgaagcagca aaaaggaatc aacgagctca cgcttcatta tcaaacgcca atctatttct
     3781 atttcattct ttctcttact ttgattggct ggtttacctt gctatgttta tttatttatc
     3841 atagatacaa gcttttacgt gcttaggcaa aagggataaa tacatccgat caaaaaactc
     3901 tccaaacggt ataatcgttt ggagagtttt attttaatca gtttttttat gttatatgtt
     3961 atatttttta ttcgttcaca tctaattcga tttccacccc gtagtgatca ctgatgattg
     4021 gactatcgat cccatcaaaa atccgttgat attttttaac ctgcattcct tcagggacga
     4081 aaataaaatc tatacgcaat ttttctgtat tttgttccca accatctatt ttcttttcca
     4141 cagtagctgc ttttcctttt tcattcgcga caacatacga atcttgaaga agaaggcaac
     4201 tttctcttac agtataataa ccttgagagt ctactggatt attgaaatca cctaggaaaa
     4261 aaagtggtac gcgagtttcc aaaaagtagt tttccaagtt tttccactca aaataaaagc
     4321 cacttttttt atctgtccac caagaaaagt ggcagctcga gacaaaaatg tcctgatcat
     4381 ctatctttgt tttcccaatc aagatcttgc gtgtctgata atcttctttc ttttgaaatt
     4441 gggagacggt aaggacctga ctttccaatg tttttttcgt aagcagtgca tttccttctt
     4501 catatttatc ataaccgatg tggctcattt cccaactcca aaaatactct tttccgtgtt
     4561 cctttaaaag ctgaactaaa cggtaggcaa aattatcttt acggataggt acttgattct
     4621 ttaccggaca gaacttcatt agcttcgcag gttctacttc ttcagattcc agtaattggt
     4681 tgatttcttg taaagcaatc acttcataat cttctaacag gatcttatct gctaattctt
     4741 gcaatttttt ctctggttct ggctcaagcc agctatgcgt gttgagtgtc atgaatttca
     4801 ttggttttct ccttgtcttc atttcgaaac aaaagggttg tgacaaaatt tttgtcacaa
     4861 cctctccagc aaatagtgtt ctcctgctaa ctcctcagta ataagctcaa tggaccagga
     4921 gctatttttt gtttggctgt ttttatcact tggtgcaggg cagctttttc acgacctctc
     4981 cactaaacag tattcaaact gctgcttctc ggtaataagc ccaaccaaac gaaaaatagg
     5041 aggagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct
     5101 ctccttacat tttaatggag ccgatcactt taccagctgt cactgtacct aattgcttga
     5161 tcactacttc ttctacttta tcgtttgtaa atacgatcat gatcgttgga tctttttctt
     5221 cgttttggat cacatcaaga tccatctcag ctaatttcgt tcctgttcct acttcttggc
     5281 cttctttgac agcaatctca aaagctggct gattcatttg gactgtatcg attcccatat
     5341 ggatcaaaac ttcgatacca tctttcgttt ccagcccaat tgcatgtttg ctagggaaaa
     5401 tacttgtcac ttttccagcg atcggcgcaa caacttctct tgatgttgga ataacagcaa
     5461 agccgtcacc catcattttt tgtgagaata ccggattttt tacttcagat aaaggaatca
     5521 cttctccaga agctgcggaa actagttctt gttctatacc taagtattgc ttactacctg
     5581 cttgtccatt tcctgtagaa accatttctg gctctggaat ctctgctcct gattgcagca
     5641 gatcttcgat atctgatttc aagacatctg cttttggtcc ataaactgct tggactccat
     5701 tgtctttgac gatcaatccc atcgcccctg ctcgtttcca ggcttcttca gtccctactt
     5761 tttctcgatc atttacactt acgcgtaacc gagtcataca agcatcgaca tctttgatat
     5821 tttcttttcc tcccagcaaa tgaatgatct ggaccacttg ctgatctgct gttcctgttc
     5881 cgctgttaga tacaacggtt tcttcactgt cattgtcgta atttccgtta cgtcccggtg
     5941 tagcaaagtt gaattttttg atcaagaaat ttgctaagaa atacgtaaca acaccaaaag
     6001 cgatcacaca caacacgaag ttgataagat ctccacctag acccgctttg atagctaacg
     6061 gtgtccgagt cagcaattcg atattcccaa atgaatggac ccgcaatggc aagatatctg
     6121 ccatcgcaaa tgcagcccct tggatcactg aataaatgac atataatggc acagcagcaa
     6181 acataaacat gaactccaat ggttctgtta cacctgttag aaagacagcc aacgctgctg
     6241 aaaaatacat tgatttatat cttgctttct tatcaggatc gacattacga tacatcgcaa
     6301 aagccattcc catcaagata cctgaagaac caatcatttg tcctactttg aaacgagccg
     6361 gcgtccagtt atgcaacaca aactcatatt gcgacatatc ccctgcacct ttaagattca
     6421 ctaaatcagt ggcccaagcc aaccataacg gatcttgccc aaatacttgt gtccccgcct
     6481 gtgcgcctga taaaatctca tacgatccgc caagctgtgt atagttgata ggaatcgtca
     6541 gcatatgatg caatccaaat ggcaatagta aacgttctaa tgttccaaat aaaaacggtg
     6601 ctagaatcgg tgcgctttct tgtgattctg caatccataa accaaaatta ttgattccag
     6661 cctgtacgtt tggccacacc acagcaagaa tcaatgccac gattgttgac cataagataa
     6721 caacaaacgg aacaaagcgc tttccattga aaaatgacaa agcatccggc aattttctat
     6781 aattatagta cttattataa gccattgccc cgacaaatcc agcgatgatc cctacgaata
     6841 ctcccatatt caaagctggt gcttccaata cacttgtaaa aaatccttta accatgatct
     6901 tcgtaccgaa cagagtgtgg gtaaatgcat cttcattcgc aagcatgtca cttgtgacac
     6961 caaagatagc cccagtgatc cgattgatta aaataaatga gatcccagct gcaaatgctc
     7021 cgcctgctct ttcttttgcc caacttcctc ctattgctaa agcaaataaa atatgaagat
     7081 taccaataat tgcccaacca atattttcaa ttactccgcc cgttgtgaca agcggcgtcc
     7141 aatcaggatt gatcaaggga attgatttcc ctatactgat catcaaccca gctgccggca
     7201 taactgctac cacaaccatt aaagcctttc caaatttttg ccaaaactca aaactaaata
     7261 tctttttcat tccgtttagc tcctttacgc aatcggtttc gttaaacgta ttatagcgtg
     7321 cgttttcttt tttgtaaatg ctttattttt tatttttctg tctataccat tctataaaac
     7381 ctataatacg tgtgttccct tctataattt tctcctaaaa atttattttt ttaagctttt
     7441 ttctatgaaa tattttcttt tgtctattga cttgtgcaat cggttgcgtt tattatgaac
     7501 gtataaaaca gctcattagg aggagaaaaa tgaaacagat caaacgacta tttgctattg
     7561 atccatggaa aatcagcacg acacaattag ataaaaaaaa tcttcgactg caagaatctt
     7621 taacaagtat tggaaacggt tatatgggga tgcgcgggaa ctttgaagaa acttactcag
     7681 gggaccacca tcaaggtact tatttggcgg gtgtctggta tcccgataag acacgtgtcg
     7741 gctggtggaa aaatggttat ccagaatact ttggaaaagt gatcaacgcc ttgaacttta
     7801 tcgcgataga tatttcgatc aacgatcagc tgatcgactt ggcttctttg gaaccagaag
     7861 acttttactg ggaattagat atgaaaaatg gtgtattatc tcgtacattc acaatcacta
     7921 cttctactaa taaagtacgc atttccttcg aacgttttct gagcattgtc aaaaaagaat
     7981 cagcttatat ctatttaact atagaaatgc ttgaaggtag cggaacagtc aaactcgtat
     8041 caaaattgga tggaaatgtt caaaacgaag acagcaacta tgatgaacat ttttggaatg
     8101 agattgatcg tggaactata gaagaaatcg gctatttgac gactaaaact attccaaaca
     8161 actttgggat tgaacaattt actgttactg cagcaatgcg ccatttcgtt aatggaaaat
     8221 cagtcatccc tgactatcac gaagatactt tagcattaca tggttgcttg tctgcagaat
     8281 tatctcaagg tgagacatgg tcagtggaaa aagaaattat cgttcttact agtagagata
     8341 ttccagaagc gcagcaagca gaaaaagctg tctcagaatt aacgaaccag acaaacaatt
     8401 atatccaagc aaaagaagag caaacagcgg cttgggcaga acgatggaag cttgccgatg
     8461 tggtcatcga aggagacgaa gaagcacaac aaggtattcg attcaatttg ttccaactat
     8521 tctctactta ctacggcgaa gatgaacgat tgaatatcgg ccccaaagga ttcacaggag
     8581 aaaaatacgg cggagccact tactgggata cagaagcgta tgctgtccct ctttatcttg
     8641 ctttagccaa acctgaagta actaagaatc tgctgaaata tcggcataat caactgccgc
     8701 aagcaatcca caacgctcag caacaaggat taaaaggcgc gctttatcct atggtcacct
     8761 tcactggtgt cgaatgccat aacgagtggg aaatcacatt cgaggagatt caccgaaatg
     8821 gcgcgattgc ctatgcaatc tacaattata cgaattacac cggcgatacg gaatatttga
     8881 aaaatgaagg actggaagtt ttagcagaaa tcgcacgctt ctgggcagac cgtgtccatt
     8941 tttcaaaacg agcaaataaa tacatgatcc acggtgtgac cggcccaaac gaatatgaga
     9001 acaatatcaa taacaattgg tatacaaata cgatcgcagc ttgggtcttg cgttacacgc
     9061 gagaaaatta tttaacttat caaaaagaag ctgctatcac tatcagcgaa gaagaactag
     9121 ccaaatggaa agatatcgca gagaacatgt acttcccagt tgacgaagaa ttaggaatct
     9181 ttgtccagca tgacacattc ttagataaag atctcatgcc tgtatccgat attcccacaa
     9241 gcgatcttcc tctgaatcag cattggtctt gggacaaaat ccttagatct tgcttcatca
     9301 agcaggcaga tgtactacag gggatttatt tcttcaatga tacttttaca aaagaagaaa
     9361 agcaaaaaaa ctttgatttc tatgaaccaa tgactgtcca tgagtcttcc ctttcgccaa
     9421 gtatccattc gattttagca gcagaattag gaatggaaag caaagctgta gaaatgtacc
     9481 agcgaacagc tcgcttggat ttggataatt acaataacga cacagaagac ggcttgcaca
     9541 tcacttccat gactggtagc tggttgtcga ttgtccaagg atttgctcaa atgaaaactg
     9601 cccatgatca gttgagattc gcaccatttc tgccaaaaac ttggaaagct tattctttcc
     9661 atatcaatta tcgcggacgt ttattgttca tctcagtcac agatgaacaa gttactttcg
     9721 agttattaga aggttctcct ctagaaattt acgtctatga acagtcttat tctttagcgg
     9781 atcgtctaga aataacgcta agaaaggagg aagcaaatgt ttaaaggggt attgtttgat
     9841 ttagacggtg tcattaccga tactgctgaa ttccattatc gtgcttggcg aaagcttggt
     9901 caggaaatcg gcattacgat cgaccacaca ttcaacgaac agctaaaagg cgtcagccga
     9961 gaagactcac tctctttgct tttagcttat ggcggaaaag aacactcttt ttcaaaggaa
    10021 gaatttgcag aattagcgaa aagaaaaaac gattattatt tagagatgat ccagacgatc
    10081 gaaccaaaag atgtcttccc cggggtcgtg cctttactgg attctttaaa agaagaaaaa
    10141 atcaagattg ctcttgcttc tgcaagtaaa aacgggccat tcctacttga aaaaatgggt
    10201 cttacacctt actttgatgc tattgccgac ccggcagaag caacaaacgg aaaacctgcg
    10261 ccagatatct tcctccttgc agcaaaagct gtcggactaa aagcagaaga atgtatcggg
    10321 atcgaagacg cgcaagccgg tatccaagcc atcctctcaa gtggcgctca accaattggc
    10381 gtgggacgtg ctgaagacct tggagaggag atcccgcttg tccctgatac gacttttctt
    10441 acgatcgatt acttgaaaga aaagtggcat gaccatggat aatccagaga tctccgtcgt
    10501 accagttcca gagactgaaa taaagaaaat cacattgaag aacgactact tagaagtcgt
    10561 tcttctcaat tacggtgccc gcctgcacca aatcttcgca ccagacaaag aaggaaaatc
    10621 agaaaatgtt ttattgtctt acgatcgttt cgaagatgtt ctaacagaca aaagcttttt
    10681 tggagcaact gtcggacctg tagccggaag gatccgggat gcttcttggg gtgagcatag
    10741 attggagaaa aactgcggat cacaccatat ccacggtggc acgaaaggct ggtctttcca
    10801 attttgggaa gtagaagttt tcaaaactcc atcatcaata ggtgtggtct tttacttaaa
    10861 agatgaattt tctacctatc caggaccaat cactgctacg atcacttacc gtttaacaaa
    10921 aaacgaatta gagatgacaa cctctgctag tagccttgtt gaaaccatct gcaatccgac
    10981 caaccatgcc tacttcaacc tttcaggaaa tggaacaaga gacatctatg aacatcagtt
    11041 aacggtcttt ttagatggtg tgcttgaatt agatcaagaa aaactgccta ctggaaattg
    11101 gacaaaaaaa gaaaaactgc caatcgactt tagaaaatcc ccaacactgc aagagatcct
    11161 tgcatgttat ccagatggac tagatgatgt atttcttttg catcatccta ggctttccaa
    11221 aacttcctta caattattcg agaagcattc tggtcgccaa atgactatag caacttctaa
    11281 taaaagtatg gtattatttt ctacgactgg attcgaagct gatttctctg tcaatggaaa
    11341 acagatgcat tcaaactacg gattagcaat cgaaccgcaa gaaatccctg atatcgttca
    11401 tttccccaaa tttggatcga tcaacctcca tcctggtcaa gaacggatca gtcagacgat
    11461 ttatcggttc tctgcacaat aacaagagaa aggtgttttt atcatggcta ttaccgtaaa
    11521 agatgtggcg aaaaaagccg gtgttgccac ctcgaccgtc tctcgtgtca tcaacgatca
    11581 tcctagtatt tccgagacaa caaagaagaa agtccgaaaa attatggatg aactaggtta
    11641 tgttcctaac atgactgccc gcaatctagg aaaacgcatt tccagtgcca tcggcgtaat
    11701 cttaccccct cttgattcaa aagagcggat cggtaaccca ttttatcttg agatcatgga
    11761 agcaatcaac gaagaagcgc ggaattatga tatgactaca gcaatcgcta ctgctaaaaa
    11821 ttttgaattg ctgttagaga acgtccagcg tatgcattta caaaaacaag tagatggttt
    11881 tattctagtg tattcagata gtcaagatac tgtcattgat tatttatacc aaaaccacat
    11941 ccctttcact ttgatcggac agccttatca tcatgagaac gaaatcgttt atgtagataa
    12001 tgataaccag cttctaggta aacaagcaac agaatttttg atccagaatg gacataagaa
    12061 tatcctgttt gtgacaaaca cgactcatga aagtttgtat ttcgaacgat atttcggtta
    12121 tcaaaaagca atgatgatgg ctggtcttcc tgcgttatcc agtgtagtac tagagcatcc
    12181 ggaggattat ctgtcctttg agaaaacgtt gaaagaaaac gaagccacag cattggtggt
    12241 cattgacgat atcttcgccc ttcgcacgat gcagttggcg caaatgtatg gctatgtcat
    12301 tccagagaca ctctccgtca ttagcttcaa caactcgatc ttctcaacat taacacaccc
    12361 ttaccttaca agtatcgata tcgatatctc cgaattagga cgtgtcgcta tgcaaaagct
    12421 gcgcgaattg atccatgaag aattgagcag tggcgtacgt ttggtcatcc ctcatcgctt
    12481 gatcaaaaga gagacagtcg ttcctttaga aaacccatac taaatagctg actcactaaa
    12541 tacgctgacc atttatccta tggtcagctt tttatgtaaa agaagatact acttctaaca
    12601 cattgtttat ctgtaaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    12661 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac
    12721 aaaaatttaa aaagcaattt tcttgagttt cttcttattt cttggagtta aacgcttctg
    12781 tcccagcctc ttttttcctt ccttcaattt tttacacgtc gacgattcaa taaaaaaccg
    12841 cctaatattg taaagacagg tgcaagaatc gagaaaaagg cgaatggcaa gtaatgcaac
    12901 gcgctaactt tcatcgttcc cataatgaat gtgccgctga ctccccacgg tacgagagaa
    12961 ttgacggtcg cacctgaatc cgctagtgtt cgtgtcaaat aggtttttga taagccaacg
    13021 tcatcaaaag aacgcttgaa tgtttctcct ggaagaatga tggacagata ctgttctcca
    13081 acgatcagat tgattccgat acagctcaag gcagtaaacc cgactaaacg agaaggtcgg
    13141 tccatctttt cttttaattc ttgaacgatg gtatcaacga tcagatattt gatcagtaac
    13201 ccacctaatg ccaatgcaag taaaatcaaa ctagcagaac ctaacatact catgatcccg
    13261 cctcgagaaa gcagttcatc tagctttgga tcaggtgtat ttgcgacata accattcatc
    13321 aagatgtctg ctgcttttcc gaccgtcaaa cttttctcat ggataaaaga aagaacgaga
    13381 gcagctgtcg atcccgctag taatgtaggg atagccggaa tttttttgat tgccccccca
    13441 aataaaataa ctagtggaat caatgtccaa ggagagatcc aaaagctcgc tttcatgttt
    13501 tggatcattt cattgatact agctagatcg gctgaatgac tgttatgtcc taatatcgta
    13561 taaaaaatca acgaaaggac gaatgctggg aaatccgtat aacgcatacc agcgatatgt
    13621 tcgaatagat taactcctcc aatacccgct gccaagttgg tcgtatctga taaaggcgag
    13681 cagttattcc ctaaaaaggc accggaaacg actgcgcctg tagtcattgc atcattgaat
    13741 ccaagtaaat gaccgatccc caaaaaagca attccaatcg tcgaaatcgt tgtaaatgaa
    13801 ctgccgacag tcactccgac aattgcacaa acaagaaatg ctgtcggtaa aaagaaacgc
    13861 acagaaacaa gtttaaatcc ataaaccata atcgtaggga tggttccaga taaaatccat
    13921 gaagcaacta agacaccaat caaaagaaaa atcaggattg gaatgatccc tggagtgatt
    13981 cctttgatga tcccgtcatg gatctcatcc catgtgaact ttttaaatct gccataaaac
    14041 aacaaacaca tgaaagctgc aaggatcggg atatgtggcg agagctggaa tccgatgatc
    14101 agtactccta ggagtgccaa caacacgatc aatacagcga tactttcttt taaagaaata
    14161 tttttattca tcgtcgatct cctatttcaa atgaaaaggc cctgccatga aaaaactatt
    14221 cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga cttatttttt
    14281 tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg
    14341 atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc
    14401 aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag
    14461 ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct
    14521 ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa
    14581 ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca
    14641 taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg
    14701 ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact
    14761 tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata
    14821 aataccatga agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca
    14881 atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct
    14941 acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct
    15001 tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc
    15061 ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca
    15121 gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg
    15181 aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat
    15241 acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc
    15301 cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta
    15361 ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac
    15421 atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt
    15481 acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt
    15541 gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg
    15601 actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata
    15661 aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac tctccttgat
    15721 tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc
    15781 gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa gacaacctca
    15841 aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag
    15901 aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg
    15961 ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt
    16021 cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca
    16081 atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa
    16141 attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact
    16201 cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt
    16261 tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga
    16321 aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt
    16381 cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat
    16441 atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc
    16501 ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt
    16561 caaatacata tgtcgcaaag taagagataa cgtttctcat gcggatatct ttgatgacga
    16621 taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg
    16681 cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg
    16741 taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt
    16801 cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga
    16861 agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga
    16921 ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag
    16981 ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa
    17041 ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg
    17101 aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac
    17161 gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga
    17221 tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg
    17281 tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta
    17341 agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt
    17401 ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg
    17461 caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg
    17521 tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac
    17581 gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac
    17641 tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac
    17701 gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct
    17761 cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca
    17821 tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct
    17881 tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta
    17941 tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac
    18001 tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat
    18061 tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat
    18121 tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa
    18181 ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat
    18241 agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac
    18301 aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa
    18361 tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt
    18421 tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc
    18481 caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc
    18541 gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc
    18601 ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac
    18661 ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt
    18721 taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta atccagctgt
    18781 gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag
    18841 atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg agattcctac
    18901 ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc
    18961 gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa
    19021 tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt
    19081 atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc
    19141 gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct
    19201 tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac
    19261 gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt
    19321 ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc
    19381 cgtgtagttt ttttatgaaa gcaggaacct gctagcatat gtaattaatt atttcttcag
    19441 gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg
    19501 taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc
    19561 gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg
    19621 cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc
    19681 ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag
    19741 gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt
    19801 tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag
    19861 aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa
    19921 aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc
    19981 acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct
    20041 acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca
    20101 gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac
    20161 caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt
    20221 cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc
    20281 tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag
    20341 gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa
    20401 ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa agacgaacaa
    20461 ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca
    20521 caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc
    20581 gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt
    20641 gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat
    20701 cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca
    20761 tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa
    20821 gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg
    20881 aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa
    20941 gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc
    21001 gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc
    21061 gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc
    21121 tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa
    21181 aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa
    21241 tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt
    21301 ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc
    21361 cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc
    21421 attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct
    21481 ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc
    21541 atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa
    21601 aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt
    21661 tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata
    21721 aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc
    21781 gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg
    21841 tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc
    21901 ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag
    21961 ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt
    22021 ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata
    22081 aaaaaagttt tataaaaagg tataaaacct tttt
//
LOCUS       DAZUKD010000043        22028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000043 DAZUKD010000000
VERSION     DAZUKD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.044"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..1369)
                     /locus_tag="ACG8N9_001359"
     CDS             complement(59..1369)
                     /locus_tag="ACG8N9_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4896970.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1381..2799)
                     /locus_tag="ACG8N9_001360"
     CDS             complement(1381..2799)
                     /locus_tag="ACG8N9_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4896971.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3039..4607)
                     /locus_tag="ACG8N9_001361"
     CDS             complement(3039..4607)
                     /locus_tag="ACG8N9_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4896972.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4611..4892)
                     /locus_tag="ACG8N9_001362"
     CDS             complement(4611..4892)
                     /locus_tag="ACG8N9_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4896973.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4889..5812)
                     /locus_tag="ACG8N9_001363"
     CDS             complement(4889..5812)
                     /locus_tag="ACG8N9_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4896974.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5809..6687)
                     /locus_tag="ACG8N9_001364"
     CDS             complement(5809..6687)
                     /locus_tag="ACG8N9_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4896975.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6674..8011)
                     /locus_tag="ACG8N9_001365"
     CDS             complement(6674..8011)
                     /locus_tag="ACG8N9_001365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4896976.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8014..9117)
                     /locus_tag="ACG8N9_001366"
     CDS             complement(8014..9117)
                     /locus_tag="ACG8N9_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4896977.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9191..10699)
                     /locus_tag="ACG8N9_001367"
     CDS             complement(9191..10699)
                     /locus_tag="ACG8N9_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4896978.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10665..11357)
                     /locus_tag="ACG8N9_001368"
     CDS             complement(10665..11357)
                     /locus_tag="ACG8N9_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4896979.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11379..12353)
                     /locus_tag="ACG8N9_001369"
     CDS             complement(11379..12353)
                     /locus_tag="ACG8N9_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4896980.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12653..13525
                     /locus_tag="ACG8N9_001370"
     CDS             12653..13525
                     /locus_tag="ACG8N9_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4896981.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14107..16305)
                     /locus_tag="ACG8N9_001371"
     CDS             complement(14107..16305)
                     /locus_tag="ACG8N9_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4896982.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16537..17529
                     /locus_tag="ACG8N9_001372"
     CDS             16537..17529
                     /locus_tag="ACG8N9_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4896983.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17589..19010
                     /locus_tag="ACG8N9_001373"
     CDS             17589..19010
                     /locus_tag="ACG8N9_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4896984.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19003..20502
                     /locus_tag="ACG8N9_001374"
     CDS             19003..20502
                     /locus_tag="ACG8N9_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4896985.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20541..21935
                     /gene="uxaC"
                     /locus_tag="ACG8N9_001375"
     CDS             20541..21935
                     /gene="uxaC"
                     /locus_tag="ACG8N9_001375"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4896986.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7042 t
ORIGIN      
        1 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
       61 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      121 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      181 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      241 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      301 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
      361 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
      421 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
      481 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaattga
      541 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
      601 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
      661 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
      721 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
      781 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
      841 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
      901 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
      961 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1021 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1081 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1141 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1201 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1261 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1321 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     1381 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     1441 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     1501 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     1561 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     1621 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     1681 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     1741 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     1801 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     1861 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     1921 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     1981 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2041 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2101 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2161 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2221 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2281 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     2341 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     2401 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     2461 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     2521 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     2581 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     2641 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     2701 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     2761 ttttactgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     2821 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     2881 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     2941 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3001 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3061 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3121 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3181 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3241 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3301 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     3361 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     3421 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     3481 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     3541 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     3601 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     3661 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     3721 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     3781 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     3841 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     3901 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     3961 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4021 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4081 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4141 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4201 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4261 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4321 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     4381 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     4441 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     4501 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     4561 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     4621 tttctcattc ttactcgata taaactgaat gccccagctg ctaaaaaagc aagtgaaata
     4681 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     4741 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     4801 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     4861 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     4921 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     4981 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     5041 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     5101 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     5161 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     5221 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     5281 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     5341 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     5401 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     5461 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     5521 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     5581 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     5641 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     5701 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     5761 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     5821 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     5881 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     5941 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     6001 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     6061 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtgtttcgat
     6121 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     6181 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     6241 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     6301 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     6361 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     6421 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     6481 atctccgccg tgagaatgca ggtaaaggac cgtcggaaac ggtccttctt tttctggata
     6541 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     6601 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     6661 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     6721 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     6781 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     6841 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     6901 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     6961 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     7021 tcgaaaaatg gatccttctg aatgctgatg tccgttcatc tatcggataa gccttttttt
     7081 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     7141 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     7201 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     7261 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     7321 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     7381 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     7441 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     7501 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     7561 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     7621 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     7681 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     7741 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     7801 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     7861 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     7921 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     7981 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     8041 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     8101 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     8161 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     8221 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     8281 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     8341 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     8401 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     8461 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     8521 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     8581 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     8641 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     8701 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     8761 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     8821 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     8881 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     8941 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     9001 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     9061 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     9121 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     9181 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     9241 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     9301 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     9361 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     9421 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     9481 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     9541 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     9601 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     9661 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     9721 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
     9781 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
     9841 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
     9901 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
     9961 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
    10021 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
    10081 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
    10141 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
    10201 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
    10261 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
    10321 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
    10381 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
    10441 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
    10501 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
    10561 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
    10621 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
    10681 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
    10741 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
    10801 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
    10861 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
    10921 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
    10981 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
    11041 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    11101 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    11161 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    11221 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    11281 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    11341 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    11401 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    11461 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    11521 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    11581 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    11641 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    11701 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    11761 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    11821 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    11881 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    11941 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    12001 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    12061 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    12121 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    12181 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    12241 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    12301 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    12361 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    12421 gtatataatc atttttatcg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    12481 caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    12541 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    12601 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    12661 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    12721 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    12781 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    12841 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    12901 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    12961 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    13021 ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt cttactgggc
    13081 catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa actttttcca
    13141 agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag attttggtgt
    13201 atttttcaaa attatgctgc cattagcttt accgtctatt gctactatct cgctttttta
    13261 tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg attcatctaa
    13321 atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga tgcaagcaga
    13381 aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg ttatcgttat
    13441 tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg tcaaaggagc
    13501 actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga
    13561 agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa atttttgtga
    13621 aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag aggttgtgaa
    13681 agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac gagtaaccca
    13741 caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa attatgcctt
    13801 attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg tgacaagact
    13861 tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata aatacaaata
    13921 ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa cagcctcttg
    13981 attaaacggc gtttatcttg ttgattctgc ggcaataaaa tcagccagca gcaaaacatg
    14041 aagtgctgtt ttttgctgtc ctcttttatt gcttgaatca gatcacaaga aaaacagcct
    14101 cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa
    14161 gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga
    14221 gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc
    14281 accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg
    14341 caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg
    14401 cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt
    14461 gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt
    14521 cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa
    14581 ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg
    14641 atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg
    14701 atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag
    14761 tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt
    14821 atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag
    14881 cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg
    14941 tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc
    15001 cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc
    15061 ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc
    15121 accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg
    15181 agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct
    15241 aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact
    15301 taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt
    15361 cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc
    15421 aaatctgtcc gaaaataagt aggaaaaaag accaatgagt cctaccagtg ttaaagacac
    15481 tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc
    15541 taatctgtaa atccaattat tataatctga tttcagtact atatacgttt tatctttttc
    15601 tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc
    15661 tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat
    15721 gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc
    15781 caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt
    15841 tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaac
    15901 ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact
    15961 gactaaatca taattattat catccataac aatcgtttct ataaaattta gttctttcct
    16021 gatttcagaa taagtggagt aatctgtata tgtcagcgga tgcttgaata tgtccgagaa
    16081 atttttggtc gaaccatacg tagaaaacga atattcaatc gttctaattt tccattcaac
    16141 gttagtttta acttgcctga gcaaattctt tttgtctgtt aaataactcg attcgatcga
    16201 ttcataggta ttctggtaaa tgaataaact aaatcctatg acaagtacgg ctcctaataa
    16261 aaacatcggt aaaaatattt tataaaatac tgtacttttc ttcattttct tcctctcctc
    16321 cttttaaaat aaaaggcttt catttttctg taaaattgat actcatctaa ttaaatgagc
    16381 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
    16441 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
    16501 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatatcgct
    16561 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
    16621 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
    16681 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
    16741 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
    16801 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
    16861 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
    16921 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
    16981 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
    17041 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
    17101 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
    17161 ctagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
    17221 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
    17281 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
    17341 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
    17401 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
    17461 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
    17521 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
    17581 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
    17641 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
    17701 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
    17761 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
    17821 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
    17881 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
    17941 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
    18001 cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
    18061 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
    18121 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
    18181 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
    18241 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
    18301 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
    18361 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
    18421 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
    18481 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
    18541 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
    18601 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
    18661 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
    18721 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
    18781 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
    18841 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
    18901 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
    18961 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
    19021 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
    19081 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
    19141 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
    19201 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
    19261 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
    19321 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
    19381 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
    19441 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
    19501 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
    19561 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
    19621 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
    19681 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
    19741 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
    19801 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
    19861 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
    19921 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
    19981 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
    20041 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
    20101 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
    20161 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
    20221 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
    20281 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
    20341 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
    20401 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
    20461 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
    20521 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
    20581 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
    20641 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
    20701 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
    20761 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
    20821 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
    20881 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
    20941 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
    21001 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
    21061 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
    21121 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
    21181 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
    21241 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
    21301 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcttacag
    21361 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
    21421 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
    21481 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
    21541 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
    21601 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
    21661 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
    21721 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
    21781 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
    21841 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
    21901 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
    21961 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
    22021 ccaaattt
//
LOCUS       DAZUKD010000044        21980 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000044 DAZUKD010000000
VERSION     DAZUKD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.045"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACG8N9_001376"
     CDS             complement(26..328)
                     /locus_tag="ACG8N9_001376"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4896987.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACG8N9_001377"
     CDS             complement(353..2446)
                     /locus_tag="ACG8N9_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4896988.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACG8N9_001378"
     CDS             complement(2935..3699)
                     /locus_tag="ACG8N9_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4896989.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8N9_001379"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8N9_001379"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4896990.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACG8N9_001380"
     CDS             complement(4704..5780)
                     /locus_tag="ACG8N9_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4896991.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACG8N9_001381"
     CDS             complement(5804..6397)
                     /locus_tag="ACG8N9_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4896992.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8N9_001382"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8N9_001382"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4896993.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACG8N9_001383"
     CDS             complement(8067..8366)
                     /locus_tag="ACG8N9_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4896994.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8N9_001384"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8N9_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4896995.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="ACG8N9_001385"
     CDS             complement(10121..11032)
                     /locus_tag="ACG8N9_001385"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4896996.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="ACG8N9_001386"
     CDS             11152..12009
                     /locus_tag="ACG8N9_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4896997.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="ACG8N9_001387"
     CDS             complement(12196..12558)
                     /locus_tag="ACG8N9_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4896998.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12747..12929)
                     /locus_tag="ACG8N9_001388"
     CDS             complement(12747..12929)
                     /locus_tag="ACG8N9_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4896999.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12904..14343
                     /locus_tag="ACG8N9_001389"
     CDS             12904..14343
                     /locus_tag="ACG8N9_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897000.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="ACG8N9_001390"
     CDS             complement(14350..16902)
                     /locus_tag="ACG8N9_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4897001.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="ACG8N9_001391"
     CDS             complement(17016..18347)
                     /locus_tag="ACG8N9_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4897002.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="ACG8N9_001392"
     CDS             complement(18313..19791)
                     /locus_tag="ACG8N9_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4897003.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8N9_001393"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8N9_001393"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4897004.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         6399 a         4691 c         3769 g         7121 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt
//
LOCUS       DAZUKD010000045        21738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000045 DAZUKD010000000
VERSION     DAZUKD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.046"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1135
                     /gene="bsh"
                     /locus_tag="ACG8N9_001394"
     CDS             161..1135
                     /gene="bsh"
                     /locus_tag="ACG8N9_001394"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4897005.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            1460..3355
                     /gene="asnB"
                     /locus_tag="ACG8N9_001395"
     CDS             1460..3355
                     /gene="asnB"
                     /locus_tag="ACG8N9_001395"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4897006.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3550..3996)
                     /locus_tag="ACG8N9_001396"
     CDS             complement(3550..3996)
                     /locus_tag="ACG8N9_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4897007.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            4224..4373
                     /gene="rpmG"
                     /locus_tag="ACG8N9_001397"
     CDS             4224..4373
                     /gene="rpmG"
                     /locus_tag="ACG8N9_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4897008.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4461..4979
                     /locus_tag="ACG8N9_001398"
     CDS             4461..4979
                     /locus_tag="ACG8N9_001398"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4897009.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5056..6012)
                     /locus_tag="ACG8N9_001399"
     CDS             complement(5056..6012)
                     /locus_tag="ACG8N9_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4897010.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            6178..6984
                     /locus_tag="ACG8N9_001400"
     CDS             6178..6984
                     /locus_tag="ACG8N9_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897011.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            6981..7970
                     /locus_tag="ACG8N9_001401"
     CDS             6981..7970
                     /locus_tag="ACG8N9_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4897012.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            7967..8971
                     /locus_tag="ACG8N9_001402"
     CDS             7967..8971
                     /locus_tag="ACG8N9_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4897013.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            9103..9645
                     /locus_tag="ACG8N9_001403"
     CDS             9103..9645
                     /locus_tag="ACG8N9_001403"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4897014.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            9665..10363
                     /locus_tag="ACG8N9_001404"
     CDS             9665..10363
                     /locus_tag="ACG8N9_001404"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4897015.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            10384..10596
                     /locus_tag="ACG8N9_001405"
     CDS             10384..10596
                     /locus_tag="ACG8N9_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4897016.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            10596..11558
                     /locus_tag="ACG8N9_001406"
     CDS             10596..11558
                     /locus_tag="ACG8N9_001406"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4897017.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            11576..11971
                     /locus_tag="ACG8N9_001407"
     CDS             11576..11971
                     /locus_tag="ACG8N9_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4897018.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            12134..12499
                     /locus_tag="ACG8N9_001408"
     CDS             12134..12499
                     /locus_tag="ACG8N9_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4897019.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            12496..14307
                     /locus_tag="ACG8N9_001409"
     CDS             12496..14307
                     /locus_tag="ACG8N9_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4897020.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(14369..14536)
                     /locus_tag="ACG8N9_001410"
     CDS             complement(14369..14536)
                     /locus_tag="ACG8N9_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4897021.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            14785..15774
                     /gene="galE"
                     /locus_tag="ACG8N9_001411"
     CDS             14785..15774
                     /gene="galE"
                     /locus_tag="ACG8N9_001411"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4897022.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            15786..17270
                     /gene="galT"
                     /locus_tag="ACG8N9_001412"
     CDS             15786..17270
                     /gene="galT"
                     /locus_tag="ACG8N9_001412"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4897023.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            17294..18274
                     /locus_tag="ACG8N9_001413"
     CDS             17294..18274
                     /locus_tag="ACG8N9_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4897024.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            18465..19187
                     /gene="tsaB"
                     /locus_tag="ACG8N9_001414"
     CDS             18465..19187
                     /gene="tsaB"
                     /locus_tag="ACG8N9_001414"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4897025.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            19171..19713
                     /gene="rimI"
                     /locus_tag="ACG8N9_001415"
     CDS             19171..19713
                     /gene="rimI"
                     /locus_tag="ACG8N9_001415"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4897026.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            19720..20184
                     /gene="rimI"
                     /locus_tag="ACG8N9_001416"
     CDS             19720..20184
                     /gene="rimI"
                     /locus_tag="ACG8N9_001416"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4897027.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            20181..21197
                     /gene="tsaD"
                     /locus_tag="ACG8N9_001417"
     CDS             20181..21197
                     /gene="tsaD"
                     /locus_tag="ACG8N9_001417"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4897028.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         6885 a         3724 c         4612 g         6517 t
ORIGIN      
        1 ttattgttgt acttttaacc tacaatattt tttgattccc cctgagtact attttttata
       61 aatgctagta aatcaatctt ctttggcctt ttttttgata aaaaaaaata ctagatgtaa
      121 gataaaagtg taaaaacgat ttgttttagg agggaaaacg atgtgtaccg caatcactta
      181 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
      241 agtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
      301 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
      361 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
      421 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
      481 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
      541 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
      601 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
      661 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
      721 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
      781 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
      841 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
      901 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaggaaa
      961 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     1021 tacgtatgaa gacagtcaaa ttactgctgt aaatataaat aaagaagact tggatcgtca
     1081 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     1141 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     1201 tcgtgtcttt ttttgctgtt ttttttggaa atagcatcca ctaatatttt tttaacacga
     1261 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     1321 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     1381 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     1441 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     1501 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     1561 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     1621 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     1681 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     1741 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     1801 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     1861 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     1921 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     1981 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     2041 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaactaga
     2101 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     2161 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     2221 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     2281 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     2341 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     2401 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     2461 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     2521 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     2581 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     2641 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     2701 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     2761 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     2821 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     2881 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     2941 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     3001 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     3061 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     3121 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     3181 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     3241 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     3301 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     3361 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     3421 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     3481 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     3541 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     3601 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     3661 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     3721 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     3781 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     3841 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     3901 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     3961 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     4021 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     4081 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     4141 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     4201 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     4261 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     4321 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     4381 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     4441 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     4501 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     4561 atacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     4621 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     4681 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     4741 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     4801 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     4861 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     4921 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     4981 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagattc
     5041 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     5101 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     5161 cactgcttta agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     5221 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     5281 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     5341 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     5401 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     5461 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     5521 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     5581 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     5641 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     5701 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     5761 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     5821 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     5881 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     5941 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     6001 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     6061 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
     6121 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
     6181 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
     6241 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
     6301 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
     6361 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattacatta
     6421 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
     6481 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaatcgag
     6541 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
     6601 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
     6661 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
     6721 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
     6781 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
     6841 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
     6901 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
     6961 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
     7021 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
     7081 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
     7141 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
     7201 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
     7261 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
     7321 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
     7381 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
     7441 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
     7501 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
     7561 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
     7621 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
     7681 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
     7741 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
     7801 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
     7861 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
     7921 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
     7981 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
     8041 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
     8101 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
     8161 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
     8221 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
     8281 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
     8341 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
     8401 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
     8461 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
     8521 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
     8581 gctaggagga ctttatgcac agaggattgg attgctatca tttggtcaag aacgcgcgca
     8641 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
     8701 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
     8761 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
     8821 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac caagtgggga
     8881 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
     8941 tatcagacaa aaacacgcgc tgaacagttg aaatttatta aaaaaggaag gccaatcatt
     9001 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
     9061 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
     9121 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
     9181 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
     9241 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
     9301 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
     9361 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
     9421 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
     9481 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
     9541 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
     9601 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
     9661 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
     9721 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
     9781 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
     9841 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
     9901 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
     9961 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    10021 tgttctgtct ggtggtgcga gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    10081 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    10141 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    10201 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    10261 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    10321 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    10381 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    10441 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    10501 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    10561 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    10621 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcaacaaa
    10681 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    10741 aatcaatcaa ccatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    10801 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    10861 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    10921 tcgctatcga taatgatgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    10981 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcggt ggtatcgttg
    11041 ctggcggaaa tttgattcac ggcgtgagcg gagcaggcgg agagatcggc catatcactg
    11101 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtgtcta
    11161 gcgctactgg tgtggtacgt ctaggtcgtt acttggcaga agaatacgca ggagattcaa
    11221 aactgaaagc aatgttagac aacggtgaag aagtaacaag taaagatatc tttgaaatgg
    11281 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    11341 tagcttgcgc caacttgggg aatacattga atccgtcaag tattgtgtta ggtggaggag
    11401 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    11461 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    11521 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    11581 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatct tttgggaagt
    11641 ctatctacga gtaatggcca agcgttcagc aacgactttg acagaagaag aatttcgaga
    11701 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    11761 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    11821 aaaagaccag cctgtctata tttacgatca aaagaaaagc ttgagcatcc gtacagcaaa
    11881 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    11941 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    12001 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    12061 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggcagaaa
    12121 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    12181 ctatagaata ctcgtggata ggttctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    12241 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    12301 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    12361 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    12421 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    12481 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtata
    12541 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    12601 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    12661 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    12721 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    12781 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    12841 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    12901 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtcag caaaagaaaa
    12961 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    13021 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    13081 aaaaatgatt tactgtatca gcttccttct aatcctgcag atgtcgatct ttcgaagata
    13141 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    13201 ttgtttacag tatcaagaga agggcgcttc gagaacgaaa aaggaaaact tgtctatcat
    13261 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    13321 cgaggaagaa accaaaagca aggcggatca atggcatggc cacggttact aacaaaagaa
    13381 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    13441 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca tttgccgatt
    13501 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    13561 catccagcac taatccctta tagatgggat tggtcgtatt tagaaattcc tcttacctat
    13621 caggatgagt cctttgcaga agctagccga gcatttattg aagggcagat aaaagaagcg
    13681 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    13741 gatccaatcc ggcaggctat tgaatggcac gttttttctg caaaggaata caaagaaata
    13801 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc agtcattcgg
    13861 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgttcga accgccattt
    13921 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    13981 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    14041 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    14101 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    14161 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    14221 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    14281 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttctttcaca
    14341 agtcttcatg cgtgttttta aataattatt aacgagaaac gcgtttgcgc attgcttttt
    14401 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    14461 cagaagcgac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    14521 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    14581 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    14641 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    14701 gttttcacca taaaagtaaa cttcgttttt ttaaattata tataatcaac aaacacaatt
    14761 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    14821 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    14881 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    14941 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    15001 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    15061 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaatgt gaataaaatc
    15121 gtgttctctt ctactgcagc aacttacgga gaaccaaaag agtctccaat cacagaagat
    15181 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    15241 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    15301 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    15361 atcattttac aagtagctct tggtcaaaga aaagcattgg ctgtttatgg agacgattat
    15421 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    15481 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    15541 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    15601 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    15661 agtgaaaaag cgagagaggt tctgggttgg aaacctgaat acacagatat caaagcaatt
    15721 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    15781 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    15841 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    15901 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    15961 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    16021 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    16081 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    16141 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    16201 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    16261 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    16321 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    16381 gaatgaatct agacggtgaa agctggggat tccaatattc tccttatgct tattacaatg
    16441 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    16501 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    16561 tgccgattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    16621 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agcagattat ccgcaagtca
    16681 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaaat
    16741 tgttgattga agcagctaac tcagtattgg aaaagtggaa agcatattcg gatgaaacag
    16801 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    16861 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    16921 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    16981 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    17041 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    17101 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    17161 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    17221 gagatgaaaa aggacaggca gcgtttgaac gatttactgc aattttataa acgtaaaaaa
    17281 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    17341 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    17401 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    17461 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    17521 ttattattta tctattcgtc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    17581 attgaaagaa acgtgggatt ccattagtga tcttccgaca gatggaacga tcgcactagg
    17641 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    17701 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    17761 tcaaaaagtc attgaacatt ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    17821 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    17881 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    17941 cgagtcagga tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    18001 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    18061 tttagtggta ccagaagata tttctgtgat gggtttcaat gatgtcagtg tagcgaaata
    18121 tgtaacgccc tctctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    18181 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    18241 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt ggggaaatga
    18301 agtgataccg cagctttttt gtgtttcagg aaggggcaaa atttgcccgc cagcagataa
    18361 tcatgtatca ttgtactagt tttgcctttg gtattttcat cctcagaata aaaaagtatg
    18421 ctacaatgga aggcgagaat gtcatagcag aaaggatcaa atatatgatt acattaggaa
    18481 tagatactgc aaaccaaact ttggcgatcg gtgtggtaga agacgaacaa attcttggac
    18541 aaatacaaac aaatatcaaa cgcaaccaca gtgtaacact gatgcctgca atcgaccaat
    18601 tgtttgcaga tcttcaaata agtccaaaag acattgaccg aatcgcggtt tctgatggtc
    18661 ccggttctta tacagggctt cgtataggtg taacgacagc aaaaacgatt gcttatacat
    18721 tagataaaga gctagtagga gtatctagtc tgaaaaccat cgcggctaat tgcatcggtg
    18781 tggataaaat tattgtgccc atgtttgatg ctcgaagaaa aaatgtatac gctggggcat
    18841 accgctggaa agaaaataga ttagaaattg tacttgaaga tgtacatatt tctgcttctg
    18901 atttgttcac acaactacaa aagctgaatg aagctattta tttcgtcggg agtgattgtc
    18961 acaaattctt ctatgaaata aaggaaatat tgcctagtgc gatgattaat acagttccag
    19021 catgggatat tccaagtggt gtaactgtgg ctcaattagg tgctttagct gaaccagttg
    19081 cagagaaaca agcttttctt cctcgctatt taaaacgggt agaagcagaa gaaaaatggt
    19141 tggagacgca cacacctgga gacgaaagtt atgttgaaaa aatttagagc gtatgccaga
    19201 agcttttttc aagataaaat gatttataca gaaaaaacaa tcgaacaaat cgatcgtcgt
    19261 tacgtactaa gagaattgac tacagaagat atcaaagagc tgctggatgt cgaacgagaa
    19321 gtctataatg gcgaattgcc atggacaaaa agtgccttta tatcagaatt gaagtcaccg
    19381 cttcctcatc tatatctagg tatctttgat aatggaagaa tgattggttt tatcggttca
    19441 agagcgttcg gtttagattg ccatatcacg aatattgccg ttcgaccggc atatcagcgt
    19501 aaacgaatcg gttcgctgct tattgacgaa atcgagaact ttgctataat gaatcgctgt
    19561 gagacgatgt cattagaagt gcgtatgagt aatcaagatg cacaaagact ttatcggaag
    19621 ttaggttttc aagcaacaac gatcaaaaaa agatattaca ctgaaacaaa agaagatgcg
    19681 ttggatatga taaaactttt agaaaatgag tgagattagt tgatttgcag aaaaaaagat
    19741 tttgatgaag cgtacttggc agaatgcgta tggatgatta gcgaaaatgc ttatccacat
    19801 ggttcacctt ggacgaaaat gcagtttttg gcagacatcc aacagcctca ctctgattat
    19861 ctgcttttag ttgaagataa ccagatctgt ggctttattg gatactcgaa agtaatagat
    19921 gaagtcgaga tcacaaatat tgctgttgca gtttcagaac aaggaaaagg gcatgcacgg
    19981 catttgttac aattactgat tacggaacaa ataaaagatt cgctgcatgt ttttttagaa
    20041 gtacgtctat ctaatgaagc agcaagaaaa ctttatgaat cagaaaagtt tcgtatatta
    20101 ggtaaacgga aatcatatta tcgcaatccg acggaagatg cgatcattat gagcagaaag
    20161 ttaaaaacgg agacgatgaa atgaatgaag aattgattct ggcgattgaa tcaagttgtg
    20221 atgaaacgag tgtagcggtc gttagaaatg gaacagagat cttatctaac atcgttgctt
    20281 ctcaaataaa cagtcataaa cgttttggtg gcgtagttcc tgaagtagct agccgccatc
    20341 acgtagaaca aatcacctta tgcttggaag atgcactggt agaagctggt gtgtcggcag
    20401 aagatttatc ggcagtcgct gtcacttatg gaccaggact tgtcggctcg ttgctgattg
    20461 gtatttctgc agctaaggct tttgcttggg cacaccaatt gccgcttatt cctgtaaacc
    20521 atatggcagg gcatatctat gcagcccgtc tggttaaacc attccagttt ccattgatgg
    20581 cacttcttgt tagcggagga catacagaat tagtctatat gcaagaagat ggatcatatg
    20641 aaatcattgg ggagacaaga gatgacgcag caggagaagc ttatgacaaa gttggaagag
    20701 tattaggtct ttcttaccca agcggaaaag aaatcgatca gctagctcat cagggaaaag
    20761 ataattacca ttttccacga gcgatgatcc atgaagataa ttatgatttc agttttagcg
    20821 gattaaaaag tgcatttatc aatttggtgc ataatgcaca acagcgtgga gaagatctag
    20881 acaaaaatga tttagcagct agctttcaag caagtgtgat cgatgtattg attactaaaa
    20941 cacttcgagc ttgtcaaaat tatccagtca agcaactgat tgtagctgga ggtgtagcgg
    21001 ccaatcaagg gttgcgtgaa ggattgcagg atgctttggc aactaaactc cctgaggtag
    21061 aactagtgat tccccctctt cgactttgtg gagataatgc tgcaatgatt ggagcagcag
    21121 cacatgtgga aatgcaaaaa agaaattttg ctagctatca gttgaatgct gatccaagct
    21181 tggttttatc tgaataaaga ggttgtgaaa aatctgccct gcatcaagta ataagaacag
    21241 cccaggaaaa acggcttctc atgtttttga cctggctgtt cttattaccg cgtatgcaag
    21301 cttttgaacg ccgtttaatc aagagttcgt aagcctgtcg tttaactctg attcacaagg
    21361 aacaatttca aaaaacagct cctcatgttt atgaacatta tgacttgtga ccgaagagtt
    21421 gacaggtaca caccgtttaa tcgaaataag ggctgtgaca cgcttttgtc aaagccctat
    21481 tttttacagc ttattcatca ttatccgttc ctctgattgc cctcactgat taatcgttta
    21541 ttgtcgagga atatcgaata atctaggtcg gatgaggaca agacgcttcc tctagttaat
    21601 ctatcagtat tttttgcaaa cagtgaatat tacataactg aaacacacat agaaaatctt
    21661 tgagtcttgc ccagtgatct aaaactgccg gatctttcag aagttttagg gaggaaaata
    21721 agcagtgacc ctgataaa
//
LOCUS       DAZUKD010000046        21613 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000046 DAZUKD010000000
VERSION     DAZUKD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.047"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..134)
                     /locus_tag="ACG8N9_001418"
                     /pseudo
     CDS             complement(21..134)
                     /locus_tag="ACG8N9_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(517..1749)
                     /locus_tag="ACG8N9_001419"
                     /pseudo
     CDS             complement(517..1749)
                     /locus_tag="ACG8N9_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            2003..2572
                     /locus_tag="ACG8N9_001420"
     CDS             2003..2572
                     /locus_tag="ACG8N9_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4897029.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2750..3190)
                     /locus_tag="ACG8N9_001421"
     CDS             complement(2750..3190)
                     /locus_tag="ACG8N9_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4897030.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3348..4112
                     /gene="map"
                     /locus_tag="ACG8N9_001422"
     CDS             3348..4112
                     /gene="map"
                     /locus_tag="ACG8N9_001422"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4897031.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4144..5067
                     /locus_tag="ACG8N9_001423"
     CDS             4144..5067
                     /locus_tag="ACG8N9_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4897032.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5143..6282
                     /locus_tag="ACG8N9_001424"
     CDS             5143..6282
                     /locus_tag="ACG8N9_001424"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4897033.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6275..7075
                     /locus_tag="ACG8N9_001425"
     CDS             6275..7075
                     /locus_tag="ACG8N9_001425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4897034.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7075..7902
                     /locus_tag="ACG8N9_001426"
     CDS             7075..7902
                     /locus_tag="ACG8N9_001426"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4897035.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7907..8614
                     /locus_tag="ACG8N9_001427"
     CDS             7907..8614
                     /locus_tag="ACG8N9_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4897036.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8714..9580
                     /gene="rfbA"
                     /locus_tag="ACG8N9_001428"
     CDS             8714..9580
                     /gene="rfbA"
                     /locus_tag="ACG8N9_001428"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4897037.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9594..10166
                     /gene="rfbC"
                     /locus_tag="ACG8N9_001429"
     CDS             9594..10166
                     /gene="rfbC"
                     /locus_tag="ACG8N9_001429"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4897038.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10188..11216
                     /gene="rfbB"
                     /locus_tag="ACG8N9_001430"
     CDS             10188..11216
                     /gene="rfbB"
                     /locus_tag="ACG8N9_001430"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4897039.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11314..12165
                     /gene="rfbD"
                     /locus_tag="ACG8N9_001431"
     CDS             11314..12165
                     /gene="rfbD"
                     /locus_tag="ACG8N9_001431"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4897040.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12200..14233
                     /locus_tag="ACG8N9_001432"
     CDS             12200..14233
                     /locus_tag="ACG8N9_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897041.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14277..15557
                     /locus_tag="ACG8N9_001433"
     CDS             14277..15557
                     /locus_tag="ACG8N9_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4897042.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15767..16573
                     /locus_tag="ACG8N9_001434"
     CDS             15767..16573
                     /locus_tag="ACG8N9_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897043.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16585..17805
                     /locus_tag="ACG8N9_001435"
     CDS             16585..17805
                     /locus_tag="ACG8N9_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897044.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17795..19381
                     /locus_tag="ACG8N9_001436"
     CDS             17795..19381
                     /locus_tag="ACG8N9_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4897045.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19420..21558
                     /locus_tag="ACG8N9_001437"
     CDS             19420..21558
                     /locus_tag="ACG8N9_001437"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4897046.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6794 a         3658 c         4510 g         6651 t
ORIGIN      
        1 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
       61 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      121 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
      181 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
      241 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
      301 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      361 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      421 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      481 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      541 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      601 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      661 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
      721 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
      781 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
      841 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
      901 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
      961 accatctact cgatgactgt caggtgctgt gaacacttct tacgtcggca tatttgccat
     1021 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1081 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     1141 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1201 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1261 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1321 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     1381 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1441 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1501 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1561 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1621 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1681 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     1741 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     1801 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
     1861 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
     1921 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
     1981 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
     2041 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
     2101 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
     2161 cttgagacaa gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt
     2221 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
     2281 agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca
     2341 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
     2401 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
     2461 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
     2521 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
     2581 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
     2641 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
     2701 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
     2761 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
     2821 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
     2881 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
     2941 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
     3001 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
     3061 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
     3121 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
     3181 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
     3241 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
     3301 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
     3361 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
     3421 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
     3481 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
     3541 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
     3601 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
     3661 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
     3721 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
     3781 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
     3841 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
     3901 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
     3961 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
     4021 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
     4081 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
     4141 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
     4201 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
     4261 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
     4321 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
     4381 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
     4441 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
     4501 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
     4561 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
     4621 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
     4681 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
     4741 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
     4801 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
     4861 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
     4921 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
     4981 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
     5041 actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt
     5101 aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt
     5161 atggttataa gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa
     5221 atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt
     5281 ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc
     5341 ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta
     5401 atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata
     5461 gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc
     5521 acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt
     5581 tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca
     5641 gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct
     5701 tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt
     5761 ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg
     5821 tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt
     5881 tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga
     5941 aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc
     6001 agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt
     6061 tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcat attgattatt
     6121 gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat
     6181 cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa
     6241 cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat
     6301 ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag ataattttat
     6361 ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc
     6421 cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca
     6481 tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg
     6541 ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt
     6601 cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga
     6661 tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt
     6721 tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg
     6781 aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc
     6841 tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga
     6901 agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt
     6961 ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt
     7021 gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa
     7081 ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat
     7141 ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt
     7201 cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg
     7261 caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca
     7321 cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg
     7381 cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc
     7441 cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa
     7501 gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc
     7561 aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat
     7621 tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg
     7681 gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt
     7741 ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga
     7801 cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg
     7861 cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg
     7921 tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca
     7981 gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga
     8041 tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca
     8101 aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt
     8161 ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga
     8221 gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca
     8281 agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt
     8341 gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat
     8401 tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa
     8461 gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag
     8521 tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac
     8581 ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt
     8641 tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg
     8701 agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc
     8761 tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta
     8821 tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca
     8881 agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga
     8941 atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt
     9001 tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct
     9061 gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca
     9121 tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc
     9181 gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta
     9241 tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga
     9301 gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg
     9361 acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt
     9421 tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta
     9481 ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa
     9541 tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag
     9601 taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc
     9661 gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg
     9721 actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt
     9781 tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag
     9841 atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct
     9901 tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta
     9961 ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag
    10021 atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa
    10081 gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa
    10141 atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca
    10201 ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc
    10261 atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact
    10321 tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat
    10381 tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata
    10441 acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata
    10501 cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg
    10561 tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa
    10621 aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt
    10681 cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact
    10741 gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga
    10801 gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga
    10861 tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg
    10921 aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct
    10981 tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg
    11041 atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt
    11101 ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact
    11161 ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca
    11221 gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt
    11281 ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga
    11341 caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca
    11401 gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta
    11461 aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa
    11521 gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca
    11581 aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac
    11641 aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg
    11701 aaattattag gcgaaaaagc agtagaagag attttagaag actattatat catacgaact
    11761 tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa
    11821 acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact
    11881 ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg
    11941 tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat
    12001 gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat
    12061 tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa
    12121 gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa
    12181 atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc
    12241 aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct
    12301 tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg
    12361 tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg
    12421 acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca
    12481 agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat
    12541 cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg
    12601 attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt
    12661 catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc
    12721 gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca
    12781 tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc attatttcta
    12841 caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat
    12901 tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt
    12961 ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt
    13021 agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac
    13081 gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac
    13141 tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt
    13201 tgaagatatc aactattcga ataactctga agtggccctg ttctatcact tttttccaac
    13261 tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact
    13321 gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc
    13381 aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac
    13441 ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga
    13501 tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta
    13561 tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc
    13621 tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct
    13681 cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac
    13741 aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc
    13801 tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa
    13861 atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa
    13921 agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga
    13981 agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga
    14041 cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca
    14101 attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt
    14161 taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct
    14221 agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga
    14281 agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt
    14341 tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt
    14401 ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa
    14461 atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca
    14521 ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc
    14581 ctttgattat tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg
    14641 gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag
    14701 agatccaagt accagaagga caaagcatct gggctccagg caattcgctt tctgatattt
    14761 ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat
    14821 ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag
    14881 cctttttagg tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg
    14941 cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct
    15001 acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag
    15061 tcaatttgtt taccggtcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa
    15121 aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt
    15181 taggaaacca caatagttat aattctattt tgaatatcta tggattgata ggatttggga
    15241 ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca
    15301 acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg
    15361 atacattgac ttcataccga tgggttccga ttatttatat cctgttggct atggccgttc
    15421 atcgatatga tgaaccagaa gttacggagg aattctatgt tatggaagag gcatatgacg
    15481 atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga
    15541 tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct
    15601 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
    15661 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
    15721 atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt
    15781 gtatttattt atgaaggaca tttatcagaa cagaaaatta ctgctacagt tttcgatcaa
    15841 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
    15901 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
    15961 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
    16021 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
    16081 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
    16141 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
    16201 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
    16261 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
    16321 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
    16381 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
    16441 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
    16501 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
    16561 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
    16621 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
    16681 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
    16741 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
    16801 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
    16861 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
    16921 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
    16981 gaatttgctg acatcggcga ccatatttat caacctgtaa aaacctattc aagtgggatg
    17041 ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt gattgtggat
    17101 gaagcattgg cagtaggaga tcttgaattt caactaaaat gtatggaaaa atttacagaa
    17161 atcaagaact cgggaaagac gattttattc gtttctcatg atattagttc gattcgccgt
    17221 ttttgtgatc gttcctattg gttgaaaaat ggaaaagttg tcgaatatgg tgatacgatg
    17281 gaagtaacga cgaattacga aaactttttg aagaaaaaat cagttaaaac agtagatcga
    17341 aataaaaacc aagtagaacg cttcgcagct ccagatatcg tagacgtgac gaaagccgaa
    17401 ttgttggata aagataaaaa tccagtggat atgctggaac aaggagaaaa actatacgtt
    17461 aaagtaacct atgaagtaaa agacgataca atcaaaaatc cagttttagg aattgctctg
    17521 cgtacagtcg acaattttta tgtgtgcgga ctcaatactc tactagatgg tatcaagatc
    17581 ccttggaaaa aaggtgagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
    17641 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatac
    17701 aagacaaaat acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
    17761 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
    17821 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
    17881 agagtttggt cctggaaacg gccgattaac gaaacatttg atcggagcaa aacaatgtga
    17941 agtgagcatt gtcgagctag ataaggaatt gttcgatttt gtcagcgagt ttgcccaaga
    18001 tggattttat ggagatatcg aaagttttga atgggcaaac tattatgctg gacaaacatt
    18061 tgattacgtt ttgtttgccg atgttttaga acatttggta gatcctggaa aaacgttgaa
    18121 gaaagttaga gaatttttga atgaagaagg agaaattcta attacattcc ctaaccttgc
    18181 gcataattct gtcatgattg atttgttcaa taatcaattg ccatgggctt cttatggttt
    18241 gctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa
    18301 agcaggatta tacatcaata ttgaagatta tttgtactta gctgtaggtg atacagaatt
    18361 aaaatcaact tatgaagaat tgccggaagc tgtacgttat gatttcaaaa tgcgaccatt
    18421 tggcgaagtc taccaatact tcttctcatt gatgaaacat ccagtagcac aaagttccat
    18481 tgcgcagccg caaaattcta attatgtgaa agtattagaa gtaatccagc aaacgaaaca
    18541 agatgaaata attcaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
    18601 tttccatgta tcagaattag cagaacgaat ggtgtttcgc ttcgcgcagc aacctagctt
    18661 tatcgaattc agcgcagaag ctgctggaga aaaactcaca tttatcgatt caaatgctgt
    18721 agtcaaaaca gtaaatgatt gttatttgtt tgatggtaaa gatttaccag agttcttttt
    18781 aacggatatt gcagggaaag aagtgactat ccattgtcac tatcgtttta tcggagaatt
    18841 gacaccaaca atgaaagaat tgctggaaac aatccgtcca atggctgaag ttacaaagca
    18901 gctttctgag aaaaatgatg aattagaaag agaaaatcat tatcttattg agaataatac
    18961 aagattagat cagacgttga aaacaaccat aaatcgctat tgcacgttgt tggaaagtga
    19021 tgaatggaca atcaaacata gattagggcg acgtaagaaa gaaacatcga aaaaaatcca
    19081 agaaaaagag ctttctctat gtattgacga aaaaatctgg gatgcagaaa caaagatatt
    19141 gaaaatcatc gggtggggaa tcgctaattc ggatcgccaa ccattgtcct ataagttatc
    19201 agccgaccaa tcaccatttt ttgaagcaat tcctgtttta cgagaagaag tcaatcaagc
    19261 aaaacagcta gctgaaggag caaaagcagg atttgaactg cggatccttt gcgagaagga
    19321 acgttcattc ttagttgaag cagtggctca aaatggtcaa tcatggatca ttgaaatgta
    19381 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
    19441 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
    19501 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
    19561 gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
    19621 acccaaagcg gttttaaggt acgtttatca gggaaaatga gaggaaaagc aatcttagac
    19681 ttccaaacag caagacatca aaatggcata gctgtgaaat taaacggtaa atatccttat
    19741 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
    19801 tatgcaagga ctcatggcgt aaaaaaagca atccgccggg tcaaactgga attgaaacca
    19861 ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aacctgatct gaaagagcaa
    19921 aggaaagaag tacaaggatt tgcctaccgc ccattgattt ccatacttat gcctgtctat
    19981 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tacttgatca aacgtatgat
    20041 cactgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtcta
    20101 gaatcctatc aagcaaaaga tgatagaatt aaagtcgttt tccgacaaga aaatggacac
    20161 atcagtctgg caactaattc tgcattggaa atggcagaag gagagtttat agcgctctta
    20221 gataatgatg acgaattacc tccatttgcc ttatatgaag tggcaaaagt gctaaacgta
    20281 catccagaac tggatttgat ctacagtgac gaagataaaa tcgatgcaga tgggaatcgc
    20341 tttgacccgc acttcaaagc agactggtct cccgatacat tgatgggaaa taattatatc
    20401 tctcacttag gcgtatatcg gacaagcatt gttaaggaac ttggcggttt ccgtaaagga
    20461 tatgaaggct ctcaagatta tgatttggtt cttcgtgtga cagaacaaat tcctgcagaa
    20521 cacatttacc acattgaccg tgtcttgtat cattggcgga cgatcccggg atcaactgca
    20581 agcaatggag aagctaaaag ctacatctat gattctggag tgaaagcatt gacagatgct
    20641 ctatctagaa gaaatatcaa gggaagcgta catcctggca gaatttctgg tttttatgaa
    20701 atcacttatg atgttttgca agaagacttg gtcagtgtga tcattccaac taagaatggc
    20761 tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta tccaaattat
    20821 gaaatcatta ttgcagataa tggaagtaca gatcctaaaa tgcaagaatt atttgcagaa
    20881 tacaagcatc agctgaaaga ccgatttatc gtcgaattaa ttgacattcc attcaattat
    20941 tcacgtatca acaatctggc agcagaaaaa gcaaacggca aatacttttt attcttgaat
    21001 aacgatacag aagtgattga acctgactgg atgacagcga tggtttctta tgcacagttt
    21061 gatcgaatcg gctgtgtagg agcaaaatta ttttatccgg atgatacaac gcagcatgca
    21121 ggtgtattat tagggatcgg tggcgttgca ggacatgcat tgaataacta tgatcgaaca
    21181 cattgtggtt actttggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
    21241 tgtatgatgg tcaaggcagc tgacttcaat gctgtcaatg gatttgatga aatattagaa
    21301 gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc
    21361 tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagataca
    21421 ccagaaaaac aaagacgttt tgctggggaa atcaaaaaaa tgcaagataa gtggccttct
    21481 tatattgctc atgacccatt ttataacgat aatctaacta aagaaggaat cggcgatttt
    21541 tcgttgagac ctgattgatt aaaagaactg tattttcatt tttattaatg aaaatacagt
    21601 tcttttaatt taa
//
LOCUS       DAZUKD010000047        20634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000047 DAZUKD010000000
VERSION     DAZUKD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.048"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..315
                     /locus_tag="ACG8N9_001438"
     CDS             <1..315
                     /locus_tag="ACG8N9_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4897047.1"
                     /translation="LFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNWV
                     YSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRS
                     KK"
     gene            complement(406..771)
                     /locus_tag="ACG8N9_001439"
     CDS             complement(406..771)
                     /locus_tag="ACG8N9_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897048.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            979..1461
                     /locus_tag="ACG8N9_001440"
     CDS             979..1461
                     /locus_tag="ACG8N9_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897049.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1658..2548
                     /locus_tag="ACG8N9_001441"
     CDS             1658..2548
                     /locus_tag="ACG8N9_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS4897050.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2874..5348
                     /locus_tag="ACG8N9_001442"
     CDS             2874..5348
                     /locus_tag="ACG8N9_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4897051.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5451..7187)
                     /locus_tag="ACG8N9_001443"
     CDS             complement(5451..7187)
                     /locus_tag="ACG8N9_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4897052.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7184..7888)
                     /locus_tag="ACG8N9_001444"
     CDS             complement(7184..7888)
                     /locus_tag="ACG8N9_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4897053.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8019..8524
                     /locus_tag="ACG8N9_001445"
                     /pseudo
     CDS             8019..8524
                     /locus_tag="ACG8N9_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            8484..8822
                     /locus_tag="ACG8N9_001446"
     CDS             8484..8822
                     /locus_tag="ACG8N9_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4897054.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8843..10261
                     /gene="ffh"
                     /locus_tag="ACG8N9_001447"
     CDS             8843..10261
                     /gene="ffh"
                     /locus_tag="ACG8N9_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4897055.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDTVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10373..10951
                     /locus_tag="ACG8N9_001448"
     CDS             10373..10951
                     /locus_tag="ACG8N9_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4897056.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10955..11476
                     /locus_tag="ACG8N9_001449"
     CDS             10955..11476
                     /locus_tag="ACG8N9_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4897057.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11555..12109)
                     /locus_tag="ACG8N9_001450"
     CDS             complement(11555..12109)
                     /locus_tag="ACG8N9_001450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4897058.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12362..12637
                     /gene="rpsP"
                     /locus_tag="ACG8N9_001451"
     CDS             12362..12637
                     /gene="rpsP"
                     /locus_tag="ACG8N9_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4897059.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12649..12900
                     /locus_tag="ACG8N9_001452"
     CDS             12649..12900
                     /locus_tag="ACG8N9_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4897060.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13024..13815
                     /locus_tag="ACG8N9_001453"
     CDS             13024..13815
                     /locus_tag="ACG8N9_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897061.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13985..14506
                     /gene="rimM"
                     /locus_tag="ACG8N9_001454"
     CDS             13985..14506
                     /gene="rimM"
                     /locus_tag="ACG8N9_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4897062.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14496..15257
                     /gene="trmD"
                     /locus_tag="ACG8N9_001455"
     CDS             14496..15257
                     /gene="trmD"
                     /locus_tag="ACG8N9_001455"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4897063.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15393..15740
                     /gene="rplS"
                     /locus_tag="ACG8N9_001456"
     CDS             15393..15740
                     /gene="rplS"
                     /locus_tag="ACG8N9_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4897064.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16050..16292
                     /locus_tag="ACG8N9_001457"
     CDS             16050..16292
                     /locus_tag="ACG8N9_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4897065.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16380..16526)
                     /locus_tag="ACG8N9_001458"
                     /pseudo
     CDS             complement(16380..16526)
                     /locus_tag="ACG8N9_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16583..16897
                     /locus_tag="ACG8N9_001459"
                     /pseudo
     CDS             16583..16897
                     /locus_tag="ACG8N9_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16926..18185
                     /locus_tag="ACG8N9_001460"
     CDS             16926..18185
                     /locus_tag="ACG8N9_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4897066.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18238..19134
                     /locus_tag="ACG8N9_001461"
     CDS             18238..19134
                     /locus_tag="ACG8N9_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897067.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19145..20014
                     /locus_tag="ACG8N9_001462"
     CDS             19145..20014
                     /locus_tag="ACG8N9_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897068.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20024..20634
                     /locus_tag="ACG8N9_001463"
                     /pseudo
     CDS             20024..20634
                     /locus_tag="ACG8N9_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6803 a         3563 c         4244 g         6024 t
ORIGIN      
        1 cttttttata gtaattttat cttaatagta gcgatattat tacttctgaa tatatggatt
       61 tttgatagga gtagaaatgc aagtataggt tttagaacta aacgaagtct gtcttctaaa
      121 aaaaattggg tatactcaca aactattttt tatggaggaa ttgttttaat tagtttactt
      181 tcttcaacac tttattcttt gaatattata gatgtttcca cctcaaattc aatatctatt
      241 attggaatca ttattgctgc aattattaca caactgtttt tagtgtttgg agagaagaaa
      301 agaagtaaaa aatagaaaaa ctagtgatag ttttggtgat ataagaaaaa catatatgta
      361 taaaaagaag aaagtagctt ctctttgtac atatatggtt tgtttttatg gatgccgagc
      421 ttttttataa gataaggcta ccgcccataa cgacccaagg atttgtggaa ctaagtaaat
      481 acctgcaaga agcaagatca gctgggtcgg ttttaaagga tgatagaagt aacgccattc
      541 agtaaaattg gcgcaaaata aataggaagt gacagtagat ataatattgg caatgaataa
      601 agatataata cgcctgttcc tttgaaaata atatccagca aaaagagata aaaatatcaa
      661 tgtgatgaac ccgcttacgg aatgataacg aaaatcaagc agtaaggcga aatgcacata
      721 aggaacaaaa gataaaagca acggaaggtt tttccagcaa gtttttatca tctttcatca
      781 ccacctaaca agtgttatta ttttgttagt tttattgtaa attgaatcct gcaataatca
      841 attacttttt cattatttct agattaaatg aataaactct catgccatta tctctaaaaa
      901 aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag
      961 tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt
     1021 tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg
     1081 gagatattag gcctgatcca aggaagcgga ttagtggttt ctaggaattt tccagctttg
     1141 actgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac
     1201 tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg
     1261 ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggattcagct
     1321 tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt
     1381 attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta
     1441 tctataaaaa ataactattg atggtggatc atcagtaaaa aagcatatga gctgcgtaaa
     1501 atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat
     1561 gatttttaca taacttaact atatttttac agcaaccata cgagggattt acagtcgact
     1621 gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct
     1681 aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc
     1741 tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc
     1801 gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa
     1861 ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg
     1921 agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc
     1981 aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac
     2041 agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa
     2101 ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag
     2161 cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca
     2221 agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag
     2281 ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga
     2341 accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta
     2401 cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga
     2461 aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga
     2521 acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac
     2581 tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa
     2641 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     2701 tttattcgaa aaaaagagct acgacataga actgtcgtag cttttttttt gctatatgac
     2761 aggaatgaaa ggtgtttgca agcttaatgt gaggaacact ttccatatct gttctatact
     2821 ggataggaga agtttggaaa tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga
     2881 ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca aagatcatca gataaagtca
     2941 tccgtctttt tccatcgtat aaagaaggat taacggcaga tcaagtcaag gaacgaatag
     3001 aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa aacaaatcag caaatcgtat
     3061 tggaaaacat atttacgtat ttcaatttga tctttcttat cttagccatt ttgctttgtt
     3121 tagtagaatc ctataagaac ttgacttttt taccagttat catagccaat acaggaatcg
     3181 ccatttttca ggaaattcga tccaaaaaga tcttggacga actaaatgtt ttaaatacag
     3241 caaaagtcaa tgtcatacga gacgggatag aacaaacaat agatatagaa gaccttgtaa
     3301 tagatgacat tgttatttta gagactggac atcaaattcc agcagatgca gaggtcgtgg
     3361 aagggaaact gcaagttaat gaggctttat tgacaggcga agcagatgaa ataaccaaag
     3421 aaataggcga tccattaatg tccggcagtt tcattgtttc cggaaaagct tacgcccgat
     3481 tagacaaagt tggagcagat tcgtatattt cccagctgac agtcaaagcc aagacaatgg
     3541 gagaaggaga acagtcggag atgattgctt ctcttaatca attgatcaaa tggattggga
     3601 tcatcattat tcctatcggt atcctgcttt tttctcaaag ttactttttt aatggaaaca
     3661 cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat cggtatgata cctgaaggac
     3721 tgtacttgct gacgacgatt gcattagcat taagtgcgac taaactagca aagcaaagag
     3781 ttcttttaca caatatgaaa agcattgaga cattggcaag ggtaaatgtc ctttgtgtag
     3841 ataaaacagg aacgatcaca gaaaataaaa tgtctgttca aaaagtgatc gtctctaaaa
     3901 aacaagaaat aatcagtcag tcagatcaat tggaagcatg cattgctgac tatgcaaaag
     3961 cgatggcagg agacaatgca acaatggaag ctgttaaagc atattttaca aaaaccactg
     4021 atcaatccta tcaccatgtt attccttttt cttctgtcca aaaattcagc agtgtagcgt
     4081 tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg
     4141 aatatgcgga agagtttgtt tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg
     4201 tgtacgatgg gattcttgaa gaaccagtat tgacagaagc agtcacacca ttaggctata
     4261 ttctgattgc taatcctatc agaaaagaag caagagccac atttgattac ttcaaaaaac
     4321 agcaagtagc catcaaagtg atttctggag ataatccgct caccgtatcc aatgtagctg
     4381 tccaagcagg gatttcacat gctgatcagt acgttgatgc atctaaatta tcagaagaag
     4441 aatatgaagc agctgtcgaa aaatatacag tttttggacg cgtcaagcca gaacaaaaga
     4501 aaatcttcgt tcaattgcta aagcgtcgta acaatacagt agctatgaca ggtgatggcg
     4561 tgaatgatat tctagccatg aaagaagcag actgcagtat agcgatggct tccggaaatg
     4621 aagccgccat gcaggcctca caagtggtct tacttgattc tgacttttca cgcatgccag
     4681 aagttgttgc agaaggacgg cgcgttgtga acaatattga gcgatcagcc agcttgtttt
     4741 tagtgaagaa tattttctcc tttttgctct cactgttttc agtgatcttt gctctgacct
     4801 atcctttaga accatcacaa atcacattga ttagtttgtt tactatcgga cttccatctt
     4861 tcttgcttgc tctagaagaa aacaaaaagc gaatcagagg gaaattcatc atgaatgtga
     4921 tggaaaaagc tgtgccaggt ggactaacag atatgatcgt agtcggtgcg ttggtgatct
     4981 gcggggtgac tttgaatcta aataaaacag atgtttctac agcatctacc atgcttttga
     5041 ttgcagtagg gttcctcgtt ctctataaaa tctgttcacc gctgaataaa ttcagaagtc
     5101 agatcattct tttctgtgct agcggtaact ttttttcggt catctttttg cataagctgt
     5161 tttcaatcac cggtatctca gctgtatcac ttttgctgtt gagcattcta tttttctcgg
     5221 cagattcgat ttttaggcat ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg
     5281 aaaaagagca aaagccgttt agtcgcgtag ttaaaaaatt tctatcgata tttaaacgag
     5341 aaaattgacg aaaagtatct caaaaaagag taaaaaagaa caaccactcg tgtatctttc
     5401 gaagcaggat gagtcatcca actttaaaac gcacgagcag ttgctctttt ttacattttc
     5461 ggaatggtaa cggtgaaagt cgaacctgtt ccaagatgac tgtctacagt aatcgtacca
     5521 tgaaggagtt gcacgtagtc ttttacaatc gctaacccta atccggtccc gcctgaatgc
     5581 cggcttcgcg ccttgtctac gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg
     5641 tcgataccta taccaaagtc ttggacagaa aacgatagat catctgttgc ctgatagcgg
     5701 ataataatct cgctgtcgct ttttgaatat tggacagcat tttcaatcag atttttgata
     5761 attggataaa ggatatctgt tttagtagta aatggttcgt tctttccaat caatcgaatc
     5821 gtcaaccgtt tcttttcgat gattggctga taattttgaa cgatttgctg gaaataaggt
     5881 tctgtataaa ttgtctggat ctcgtaagaa ttctcgccat cttttgacag ctggatgatt
     5941 tcccgaatca gtttatctaa ccggatcgca tctttttgca tgatctcaag aaaagaagtc
     6001 agagtttgcg ggtcatcttt tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa
     6061 gtcacaggag tttttaattc gtgagagacg ttgccaacga aatccttttg tagcttttct
     6121 aaacgtcgca ctttcgttaa gtcatacgcc acccctagaa tctgtccggt accttctgaa
     6181 ttagtaaagt agcgcaaact caagtctaat gtctgccgac cttctgtaag ggtgatctcc
     6241 tgatggatca gcggatgctc aggagtcact tgatgaatca gctgaatgag ttttggctct
     6301 tgaatgactt ctgtgtaccg ctgttcaggt tgatagctta ccacgtttaa atgtagctgc
     6361 atctttggat taagcagtag taaacgagaa tcagcatcat caatcaggaa tacgccaatc
     6421 atcagttcgt tcagtaacgt atataattgc tcttctgtgg tagtatacgc ccgatacatc
     6481 ttactcattt gttcgctcaa cgcattgatc gtctgataca attcttccca ttgatcgggt
     6541 gtttgcatga ttacttccgc ctgattagga ttttgcacca ttttttttag tacgggcagc
     6601 acggttttca atggctggtt tttttgatag atcagataat agatcattcc tgcaagaaca
     6661 aggaagaaag acagaaagaa gaaaaagacc caacggcgaa aactctcagt tctcggtaaa
     6721 aaaccgcttg tcggttctgc gatacgtaaa atggcttcta actggccact ttttttgact
     6781 ggtaatgcga catataaaag atccccattc aaagtagtac ttttgcgcaa agcgctacct
     6841 aggttcccac cattcaatac ggctttgatt tcgggacggg agtttcgctg ttcattcaat
     6901 gtttcattgt ttgtgtcaaa taagatcttt ccagtagcat ccattaaagt aatccgttcg
     6961 ttcgactgat ggacgaattc ttccaaagca gttttgtttt gcgctgcttg gaggttttcg
     7021 acatctaatt gccggatcag cagttgtgct ttttttgtca ggtagctttc ttgctgttcc
     7081 acgatctgct gttgaaaata atgggaaatc aattgccagc caccaaaaaa caaactaact
     7141 aaaagaataa gaaagaagag acgttcataa atttttttcc atttcatcgt ttgggttcct
     7201 gaaacttata gccaaatcct ctaatagtca ccaagtactt tgggtgtttt ggatctcgct
     7261 cgattttttc tcgcaaatga ctaacgtgta catctacgat tcggctttgt cctgcgaagt
     7321 caaaattcca aatgcggtct agcaatgtgt ctcggtcgat cacacggtct tttcgtttca
     7381 tgaaataaac aagtaattca aattctttcg gtgttagttc aatcagatgg tcgtctacgg
     7441 tcacttgata attggtcaaa tcagcagtga tttgtccaat cgatagatat tcaggctcag
     7501 cttcatcttt tgattgctta cgaggttcaa ttcgtctgaa tattgctttc atacgggcta
     7561 atacttcgcg aggactaaat ggtttggtca aatagtcgtc agcacctatt tctaggccaa
     7621 tgatccgatc cacttggtca tctttagccg tcagcatcaa aatcggtgtt tcattttttt
     7681 cttgacgcag ccgctgagtg atctccatgc catccatcgt tggaagcatc acatctaaaa
     7741 tgatgaaatc aaatgattgt tctaatgcta attctaatcc ttcgcctcca tctgtggcac
     7801 tggttacctt gtagccttct ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt
     7861 catcatcaac aactagtatt tttttcattc aaatctcctt taaataggtt tcattctgtt
     7921 tatattttag catagaatca gtacaaaggg ctattttaca aaagaaataa atggtataat
     7981 aggaaggcac tttgctgaat agaaaggaag agcagtcaat gattggaatc agtgcatgtt
     8041 taggcggtct ggcctgtcgc tatgatggac agaaaaaaga agtaccagaa ttgatggaac
     8101 tagttgaaac tggagaagct gtcatggtct gtccagaagt gatcggcgga ttaccaattc
     8161 ctagagatcc cgctgaaatc attggtggaa atggattcga tgtgtggaaa gatcaagcaa
     8221 aagtgtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     8281 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     8341 ttcatgcgga acccattcga tttatgacgg gagtttttcc ggaataaaaa aaaatggtgc
     8401 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     8461 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     8521 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     8581 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     8641 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa cttcatcttt
     8701 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     8761 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     8821 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     8881 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     8941 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     9001 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     9061 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     9121 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     9181 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     9241 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     9301 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     9361 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     9421 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     9481 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagata ccgtcaacgt
     9541 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     9601 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     9661 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     9721 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactatga
     9781 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     9841 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     9901 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     9961 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
    10021 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
    10081 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
    10141 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
    10201 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
    10261 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
    10321 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
    10381 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
    10441 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
    10501 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
    10561 tattaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
    10621 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
    10681 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
    10741 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
    10801 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
    10861 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
    10921 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
    10981 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
    11041 tccaacaggt aagcaaacca cttatgaagc tttttatgaa acaatcgata cgaccatcat
    11101 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
    11161 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
    11221 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
    11281 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
    11341 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
    11401 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
    11461 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
    11521 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
    11581 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
    11641 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
    11701 cattcaaaaa gacaagtgat tgtcgtagat ggtggtttgc gccaaagccg ccgatcccac
    11761 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacgctgtgt ccataaggtc
    11821 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
    11881 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
    11941 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    12001 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    12061 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    12121 tccttcaaac tatctataac aatcttacaa aaaataagta actggagcaa ataaaaacac
    12181 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    12241 atcttctatt tcctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    12301 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    12361 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    12421 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    12481 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    12541 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    12601 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    12661 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    12721 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    12781 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    12841 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    12901 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttat taattgaaga
    12961 aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag
    13021 ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg
    13081 gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt
    13141 ccagaaaaaa gtagtaccaa aacggctacc accactgaac aaacaatggc tacctcaaat
    13201 aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct
    13261 ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt
    13321 tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat
    13381 caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca
    13441 atccaagttt tgcagccctt tgagatcgac acaaatggtc agcaagtgga cttaggttct
    13501 cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga
    13561 aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct
    13621 aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa
    13681 attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg
    13741 aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct
    13801 atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag
    13861 gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga
    13921 aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata
    13981 taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga
    14041 agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgtt
    14101 aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca
    14161 taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata
    14221 tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt
    14281 ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt
    14341 aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc
    14401 tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt
    14461 ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat
    14521 ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag
    14581 gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag
    14641 tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg
    14701 ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg
    14761 acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact
    14821 tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag
    14881 atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga
    14941 tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg
    15001 attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag
    15061 ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat
    15121 taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat
    15181 ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag
    15241 cagatgaagc caagtgactt tttctaaaat attctttaca gtaggttgat aatatgttag
    15301 tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctatg agaaaagctc
    15361 ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa
    15421 aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg
    15481 caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac
    15541 gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg
    15601 ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg
    15661 gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta
    15721 tcaaagaaat ccgtcgttaa tcattgttat cagactgaga aagaaccctt ggtattcaag
    15781 ggttcttttt tcttagcatg taatttgaac accctgatcc agtttttttc tttaattatt
    15841 ggtaggggag aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc
    15901 actatgataa tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac
    15961 ataaatggag tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc
    16021 aagaaaaaat gaaagtttga gacacattca tgaatgatgg aaaagtaaaa caagtaccaa
    16081 gttcaacaaa aaagaaaaac atcctattga aagaagtctt gaaacgcttt gaccatggag
    16141 tgacttatac agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt
    16201 atgtcgaaca aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc
    16261 gggcatatca gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa
    16321 gttcaagtat gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc
    16381 tataatacaa agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag
    16441 cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa
    16501 tcaattggat gcgttacatg aataaagata attctctttt atttgtacga ataaatacca
    16561 aaacgtacct cctaaaatta gattcaaaga agatagcaaa gaggaaaaag atagttctgc
    16621 agcagctata gcggcatgtg gattgctaga actttccaaa caactgcctg tttcagatcc
    16681 tcgtcatttc gattacgaag agatagcaat caaaatcttg gaagaattac agaaaaacta
    16741 tacaacgaaa cgagttaacg gatcaaacgg tctgcttctc catgccgttt atgataaaaa
    16801 cagcttaaaa ggtgtagatg aatgtgtgat ctggggcgat tacttttatg tagaaggaat
    16861 cactcgtcta gctaaaacat ggtactgtta ctggtgatgt ataagagaag aggaggattc
    16921 atataatgaa gcgatggaaa aaaatgatga ttggatcaat agttttagga atgtcactcg
    16981 tatttggtgc ttgtggtaat caagaagaca gtagtgaaga atcagcaaat gaaattaccg
    17041 tttcgacatg gaattatgaa acaacacctg aattcaaagc gctctttgaa gcattcgaaa
    17101 agaaaacggg aatcaaagtg aaagcagtag atattgcttc agatgattat gatacgaaac
    17161 tcaccacgat gctttcttct ggagacacga cagatgtatt gacgatgaaa aatcttttgt
    17221 cgtattcaaa ttatgcttta cgagatcagt tagtcgatca aacagaacga attagagaat
    17281 tagacactga tgctgctgaa gaaacatatg aaatgtatga tatcgatgga aaaacatatg
    17341 cattgccata tcgaacggac ttttgggtac tatattacaa taagaaaatg tttgatgatg
    17401 caggaatcga atatccaaaa aatctaactt gggacgaata tgaagattta gcaaaaaaac
    17461 ttagtaaaaa tgacggacaa gtttacggag catatcaaca tatctggcgt tcgacaatcc
    17521 aagctattgc agctgctcaa aacgaggcga atcttgttga accagattat caatatatga
    17581 ctgattatta tgatcgagca ttgaggatgc aaaaagaagg cgcgcagatg gattttggca
    17641 cggctaaatc aacgaaagta acctatcagt ctcaatttga agaacaaaaa gcagcaatga
    17701 tgtacatggg aacttggtat atggctggca tactcgcaaa caaagaagca agtaagacag
    17761 aagtagaatg gggaattaca gctattcctc agaagaaaaa aggagaatcg gtcacgacgt
    17821 ttggctcacc aacagcattt gctgtcaata aaaactcaaa gaaacaaaaa gcggcgcaag
    17881 aatttattga atttgcagcc agtgaagaag gagcaaaagt attagcagga gtaggagtag
    17941 ttccttcata tcgtacggat gagattgatc agctttattt taatctcgct ggaatgccaa
    18001 cggatgaaat ttctaaaaaa gctttttctc ctgatgaaat caaacttgaa tttccgattg
    18061 atacgtatgg gcctgcaatc gataagattt tacaagaaga atatgactta gttttggttg
    18121 gagatgaaac gccggaaaaa ggaacggtaa atatggagaa acgtgtagct gcggaaaaag
    18181 aataatcatg aaaggagaga agccaagaga taaaacactc ttggtttcgt catcgaaatg
    18241 gagaatagta taaataagaa agctacaaat catttgcgta gaaaacgaac gtggatcgct
    18301 tattcgttta tcgccccgaa tttcattggt tttttcgttt ttacattaat accaatcctg
    18361 ttttcgcttg tactagcatt tatgaaatgg gattcttttt caacgccaga atttgctgga
    18421 ctagataact ttcgtaaaat ggccaacgat gagacatttt ggatttcttt gaaaaatact
    18481 tttttatata ccgctgctgt agtgcctggc acacttgttt gttcgttagg gctagcttta
    18541 ctactaaatc agaaaattag agggatgaag ctttttcgta cgatattttt ctttccgtat
    18601 gttacttcct tagtagcaat tgctgttgtt tggaatatgt tgtttcatcc aacagtggga
    18661 cctattaata tctttttaag acgatttatt gaacatcctc caggttggac ttcaagttca
    18721 aattgggcgc tttgggcaat tgtaattgtc agtatttggc gagggatggg ttattacatg
    18781 attctatacc ttgcaggact acaaagtatc caaaaagaac tatatgaagc gtcttcgcta
    18841 gatggagcaa accgttggaa acaatttttg aatgtgacat taccttcgtt gagaccaaca
    18901 accttctttg taacgatcat gcttgtcatc aattgtttca aggtgtttga ccttgtgcaa
    18961 gtgatgacgg atggcggccc tggtcgagcg acaaatgtgc tggtttacca aatctacaat
    19021 gaagcattca aaaaatttga ttttggttat gcttcagcta tttcacttgt tttgttcgta
    19081 attgttttgg gcgttacggt tgttcaattt tcttggaata agcgtcgtac ttaggaggtg
    19141 tgatatggaa gagagtgttc gaaatgaaag aaaaaaagca aaaattcgtt caaaaaacat
    19201 gccctttatt caatggttgg gtatgattct gctgattgta ttagctttat tgacgatcat
    19261 tcctttttta tggatggttt ctgcttcatt caaagcaaat aatgaagtgt taacgattcc
    19321 gattcaatgg atccctaaaa cttggcattt agagaattat ctggttattt ggtctcgtat
    19381 tcctttagtg acatttttta agaatacagc ttttttaagc gttgtgatca cgttgattca
    19441 gttgtttact tctagttttg ctgtttacgg gttttctgaa ctaaatttta aagggcgcga
    19501 tattttattt ttagcatata tcggcacgat tgctgtccct tggcagtctt atatgatccc
    19561 gcagttcatt atgatgagaa aagtgggttt aacagatacg ttgtggtcgt tggtgctatt
    19621 acaagcgttc aatgcttttg gtgtgttctt gttgaagcaa tattacagta gtatcccaga
    19681 ttctttatgt gagagtgcgc ggatcgatgg cttatcagag tgggaaatct atcgcaagat
    19741 cattttacct cttacaaaac cggcgattgc cagtttgacg atcatcacat tcgttaatac
    19801 atggaacgat tatatggggc cattcattta tttgtcttct acggaaaata aaacaatcca
    19861 gttagggttg aaaatgttta ttgggctatt tgatgcagag tacgcattaa tcatggctgc
    19921 ctcagttgtt tctgttcttc ctgttgcttt tatattttta gcgatgcaaa aatattttgt
    19981 agaagggatc gcttcatcag gaatgaagga ataggaggtg attatgaaag agcagcgttt
    20041 tgacaaaaat gtttatatgg aattctttta ttggttgtat caatcattgg caagtagcct
    20101 ttgtttttgg tgtgtcaata tcttgtatat agcagctgcg ttccttttgt cgatagatgg
    20161 aaggaatgtc ttttccttta ttttttcttt actttttatt ggtccaggtt gcatcgtgat
    20221 gataggtatg acagaagaat tagcaaaaga aaaaagtttt tcaccagtac gtttgttcat
    20281 aaagacgttt cgccaatttt ggctaaaggg atttttttat tggctatttg cttggatagt
    20341 tagtgtgata atgatttttg attgtttctt ttttatacgt ttttcttatg gaaaatggtt
    20401 gattccgttg tttgttttac ttgcttgttt tacttgcttg tttatcagtt tcctttagca
    20461 tcaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa gttctacgga
    20521 tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt ttactattga
    20581 caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta ggtc
//
LOCUS       DAZUKD010000048        19774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000048 DAZUKD010000000
VERSION     DAZUKD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.049"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..1312)
                     /locus_tag="ACG8N9_001464"
     CDS             complement(287..1312)
                     /locus_tag="ACG8N9_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4897069.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1587..3272
                     /locus_tag="ACG8N9_001465"
     CDS             1587..3272
                     /locus_tag="ACG8N9_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4897070.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3286..4569
                     /locus_tag="ACG8N9_001466"
     CDS             3286..4569
                     /locus_tag="ACG8N9_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4897071.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4639..5508
                     /locus_tag="ACG8N9_001467"
     CDS             4639..5508
                     /locus_tag="ACG8N9_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897072.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5505..6341
                     /locus_tag="ACG8N9_001468"
     CDS             5505..6341
                     /locus_tag="ACG8N9_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897073.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6367..7434
                     /locus_tag="ACG8N9_001469"
     CDS             6367..7434
                     /locus_tag="ACG8N9_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4897074.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7431..8258
                     /locus_tag="ACG8N9_001470"
     CDS             7431..8258
                     /locus_tag="ACG8N9_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4897075.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8303..9328
                     /locus_tag="ACG8N9_001471"
     CDS             8303..9328
                     /locus_tag="ACG8N9_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4897076.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9325..11508
                     /locus_tag="ACG8N9_001472"
     CDS             9325..11508
                     /locus_tag="ACG8N9_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4897077.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11508..12638
                     /locus_tag="ACG8N9_001473"
     CDS             11508..12638
                     /locus_tag="ACG8N9_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4897078.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12635..13312
                     /gene="pgmB"
                     /locus_tag="ACG8N9_001474"
     CDS             12635..13312
                     /gene="pgmB"
                     /locus_tag="ACG8N9_001474"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4897079.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13396..13923
                     /gene="msrA"
                     /locus_tag="ACG8N9_001475"
     CDS             13396..13923
                     /gene="msrA"
                     /locus_tag="ACG8N9_001475"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4897080.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14124..14810)
                     /locus_tag="ACG8N9_001476"
     CDS             complement(14124..14810)
                     /locus_tag="ACG8N9_001476"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4897081.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14890..16428
                     /locus_tag="ACG8N9_001477"
     CDS             14890..16428
                     /locus_tag="ACG8N9_001477"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4897082.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16548..17015
                     /locus_tag="ACG8N9_001478"
     CDS             16548..17015
                     /locus_tag="ACG8N9_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4897083.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17549..17977
                     /gene="rplM"
                     /locus_tag="ACG8N9_001479"
     CDS             17549..17977
                     /gene="rplM"
                     /locus_tag="ACG8N9_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4897084.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17993..18385
                     /gene="rpsI"
                     /locus_tag="ACG8N9_001480"
     CDS             17993..18385
                     /gene="rpsI"
                     /locus_tag="ACG8N9_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4897085.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18473..19699)
                     /locus_tag="ACG8N9_001481"
     CDS             complement(18473..19699)
                     /locus_tag="ACG8N9_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4897086.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
BASE COUNT         6372 a         3575 c         4167 g         5660 t
ORIGIN      
        1 tttagagata agtatcccca aataacatgg attgaaataa gggtaaatgt gtttaaaaaa
       61 acgaactata aaaacttata cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa
      121 aaatagttta acacaaacat tatagttatc cacagctatc taaaacagat ggaaaataag
      181 aaaaattcgc taaaataccg ggagggggta acgagggatg agtgattgaa aaaagaacag
      241 agtaactgac ttatcctcag tgtttctgtt cttttataat tctgttctac atttcttgtt
      301 gttggatcga atctgtgaga gtaatctcgg ttgaaagaat gattcttttt tcttcagggt
      361 attggtggtg gattagttgg aataaaagtt ctgtggcttt gattcctaac tgatttgtcg
      421 gaatgtctat ccgagtcaac tgtggcgaaa atagggagga caataaggga tcactgaatc
      481 ctgcgatcga aagatcatca ggtatctgct ttccagattg ttctgccact ttgtaaatac
      541 tcaatgattt taatgcatcg gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta
      601 aatcattcat tgctgccatg ctatcttcta agcgaaagcc gccgtctata atccattcat
      661 tacggatatc taggtggtga tcaaataagc attgacggta accattgaga cgatcattgg
      721 atacataaac ttctgtcgga gcatggagac agccgatatg acggtgtcca ttcttgatca
      781 gtgtttccac caaatcgtaa ctatcctgga aattatccgt gtccactgag tagatgttcg
      841 tgtagtcgtt tccgactttt ccgattacca caataggaat attgtattga tccaattcaa
      901 aaagaagtgt ttcatccatc gtagagctta aaataataat tcctcggatc attttttctt
      961 ggatcttttt cgcgcatttt tctagctctt cttctggtgt ttttgccgtt tgtagaatca
     1021 agtcgaagtt attttgttca gaaacggcag aaatggattg aataacatct gaaaaaaaag
     1081 gattgccata ggtcgtatga gaagtacgat gagataatgc tagaatcgcg tcaaaaccat
     1141 ttcctgacag tcctcttgca attttgctcg gttcataatt caattctttg attgcttgaa
     1201 gtacacttgc ttttgcttta tcagagatat ttggctggtt atttaatact ctggagacag
     1261 ttgattttga gacattagct tttttcgcaa tgtcataaat agtaggtttc aattttatat
     1321 gcacctcctt tttgggagcg ttttagaata attccatagt acaatagaca ttcttttttg
     1381 tctatgattt tgaaaattta ggaatagctt gcatgatcat gagagaccat atttttttaa
     1441 gaagctatta attattgata cataagcttt cttatttgta tttttgtgta tatcaaaatt
     1501 atttaaaaaa atgctattga cagtaagcgt tttccataat atgatcaaat cgttgggaac
     1561 gctcccaaaa aagaaagggg aatccgatgt atacaaaact tttacaagcg atttatcgcg
     1621 actcagattt gtctgagaaa accgcctcta tgattgaaca aaaagtcagt gtttatcagc
     1681 agaagaaagg ccaggcaggc aaactgcatt gggacgaaga agatattttc ttgattactt
     1741 atggggatca attttttgaa gagcagcaac caactttgcg tacatttaag aaattttatc
     1801 aaaagtattt ggcagataca tttagagttg tacatttctt gccatttttc ccatatacat
     1861 ctgatgatgg tttctccgtt gtaaattatc aggcaatcaa cgaccgtttg ggcacatggg
     1921 aagatataga agaaatgaga agcgaaacac ggttaatgtt cgactttgtc tgcaatcata
     1981 tgtctgccga aagtacttgg tttcaggaat atttaaactt gaatgatacg tacaaagatt
     2041 tttttattga tttgccactg gatacagata caagcagcgt gactagacca aggaccagcc
     2101 cgttacttac aacgttcatt gacaaaaacg ggaaagaaaa acacgtatgg acgactttta
     2161 gcgaagatca agtagacttg aattttaaaa atccagaagt tttattgaaa atgatcgata
     2221 ttcttttgtt ttacttagaa aaaggcgctt catttattcg cttggacgct gtgggatttt
     2281 tatggaaaga actaggaact tcttgtatac acctgccaga aacgcataaa atcatccaat
     2341 tattccgtgc aatcgctgat gaagcagctc caggaacggt attgatcact gagacaaatg
     2401 tgccacacaa agataatatc agttattttg ggaatggcga gaatgaagca cagatggtct
     2461 atcagttttc gctaccacct ttgacccttc aagcgattcg ttcgggtaat acaagtaagt
     2521 tgaaagaatg gttaaaaacc atcgatttcg catcgaaaaa aacaactttt tttaattttt
     2581 tagcctccca tgacggtatc ggattgaatc cggtacgagg tattctgccg gaaaaaacga
     2641 tccttgaatt agtggaacag ctgaagaaag agggtgcccg agtaaactac aagaaaaacc
     2701 cagatggaac gaccagtcca tatgagataa acgctacgta tctagatgcg ttaagttctc
     2761 caacagatac gacggaatgg agaagcaaac gctttttagc tgctcattca tttttggtaa
     2821 cgttaccagg cgtgccggca atttatattc aaagtgtgct gggaagtcga aatgattaca
     2881 aaggcgtaga aaaaacagga gagaatcgaa aaatcaatcg gcaaaaattt ttgttagcag
     2941 atattgagaa agagttgcag acagatccat taagaacagc agtatatggt gaattgaccc
     3001 agatgctatc tattcgaaaa aaagaaaaag ccttccatcc gaatagtttg atttacttac
     3061 tggactcagc agattctttt atcagtttta ttcgcgaagc gaaggaagag aaagtcttag
     3121 ttatccataa tttttccaat caatcagcag gttatctttt accaaaagga gattacctag
     3181 acctgttttc tggacaaatt atccattgca gtcaagaacg aaagttgata ttccagccgt
     3241 atgaatatat gtggctgaaa ataataaaat aagaggtgga aaaaaatgaa aaaaattatt
     3301 gttactacct gtctgctagc tactggattt cttcttactg cttgtggtga aagtcaaagc
     3361 tctaacgaat cacaacagac aacgattcaa ttcatgcact cttctgtcga acaggaacga
     3421 ttaagtgtga tcaatgaact ggttgctgat tttgaaaaag aaaatccaga catcaaaatc
     3481 aagcaagtgc cggtagaaga atcagcgttc aatacaaaaa tcgttacgtt agctcgttcc
     3541 ggtaaattac cagaagtaat ggaagtcagt caggattttg ctaaagtgat ggataaagat
     3601 gaactgattg atcagacggc agtccaaaac gttatcgagg aagctggaga agataattat
     3661 tatgacggag ccaaaaacct tgttcgttca gaagatggaa aaagctatat tgctgcacca
     3721 attagcggct gggttcaagg tatttggtat gacaaagaaa aattatcaga agctggtttt
     3781 tcagaaccag aaaattggga tgatatttta aaaattgctc aacatttcac agataaagag
     3841 aataaacaat atggaattgc tatgcctact gctgaaggaa caatgagtga gcaagcgttc
     3901 tctcaatttg ctttgtcaaa tggagcaaat gttttggatg atgaaggcga ggtaacgatc
     3961 gatacggaaa agatgagaga agcattgtcg ttttatcaaa acttatccca atacacgatg
     4021 ccaggctcta atgatgtgac agaaatcaaa gatgctttca tgaatggaac agcaccaatg
     4081 gcaatctatt caacttacat tttgccttct gtttatgaag cggggaactc agaaaatatc
     4141 ggttttgcta tcccaacaca aaaagatcaa gctgtctatg gcacagtatc tggattgacg
     4201 atttcttctg gtctagacga gaaacaaaaa gaggctgctg aagcattcac agctttctta
     4261 tctcaaccga aaaacatgga aaagtgggtc ttgatgtctc ctggtggtgc tcaaccagtg
     4321 aataaacaag tagtcgaact ggatggttac aaagaaaatg aagtcatcaa gtcttttggc
     4381 gagctgccat ctgagattgc tagtgctttt gatgaagtac aagtctttgg actagtcggg
     4441 gaaaagaatt tcacgaaaat gggagatatc acaagctcag gtgtgatcgg aaaagcagtg
     4501 aaccaagtaa ctgtcggaaa cgaagatgtc gatcaagctt tagctgacgc acaaaaaaat
     4561 acgaaataac aagaagttct gccaatggtg ttcgaaaaaa gtagatagct attggcagat
     4621 tatcaggggg aggtttcaat gaatcgtcca aggaaacatt ctgacttaaa attagccatg
     4681 ggacttttag caccaagcat gcttttatta gctgttttag tgctgattcc aatgctttcc
     4741 aatattcaga tcagtttttt cgatatgccg ctgaatccta actcagacgc cgtttttatc
     4801 ggattaagca attatcttga aattttaagt gatagtgaat tttatcgttc tttaggttta
     4861 acagttgctt acacattctt agttgtagca ggcagtacag ctttaggatt agccgtagct
     4921 attttgctta accgcaagtt cagatttagg aaaacggtgc gttcactgat tttattaagt
     4981 tatgttacgc catctatttc tttgattttt gcttggaaat atatgttcaa taatagttat
     5041 ggaatcgtca atgatctgtt agtagatagg ttgcagcttt tcaacgaagc accgctgtgg
     5101 ttcgaccagc cagttgctag cttctttatg gtcgttttgt ttgctatctg gagatactat
     5161 ccgtatgcgt ttatttcatt tttggctatt ttgcaaacag tcgacaaaac gctttatgaa
     5221 gcagctgaaa tggacggtgc aaatgcttgg cagaagttca agatcgtgac actgccggca
     5281 atcaaaccag tactagcaac tgtgattgtt ttacgatcta tctggatgtt ctatatgttt
     5341 acagatgtct atctactgac gaataaagtc aatatcttag gcgtttattt gtatcagaca
     5401 gcctttgcgt tcaatgatct tggaaaagca gcagctatat cgattctatt gttcatcatt
     5461 attgcagcgg tcgtcttgct ctcacgaaag aaggtggact tgaaatgaaa aataaaaaga
     5521 gcaggaaaat cttattttac gcgacgattt tgttgctatt agctgtcaat ttgtttccat
     5581 ttttgatcat gctgatgaca tcattcaagg attcacaaga agcaattgca acgacacaaa
     5641 ctatttttcc gaaagaatgg acttttgaac attatcagga tattttcaat gctgagattt
     5701 ttccattcat catgtatttc aaaaatagtt tagtgatctc cttgatagcc tccgtaactg
     5761 cagtagtgat tgggatcagc ggggcatacg ctttgtcaaa actacgcttc aaagctcgct
     5821 cgaaaatcaa tgctagcttt tacaccgttt atatgttttc cggtattttg atcgttgttc
     5881 cattatttaa aatcatttcc ggtctaggtt tgtatgatac caatacagca ctgattatca
     5941 cgatggtcgt tcaaacactt cctactgcga tattcatgct aaaaagttat tttgacacga
     6001 ttccgactga tattgaagaa gcagcaatga tcgatggtct tagccggatc cagatcatta
     6061 ttcggatcat tataccattg gctatttctg gaatcatttc tgtcttcgtt tattgcttta
     6121 tggtttcgtg gaatgattat ttatttgctt cgattttcct gtcgacctcc gaaaaattta
     6181 ctttaccaat cggtttgaac acattattca gtacaccaga ctacatttgg ggacgaatga
     6241 tggccgcttc attggtgacc gctttaccag tcatcattat gtatgcgtta tctgaacgat
     6301 ttattaaagg tgatttagtc gaaggcggcg taaaaggata aaaagaaaag aaagaggaaa
     6361 gaaaagatga aaaaattagt tgcattagaa ccaagaatag ccggattcaa agattacgaa
     6421 gaccgtgaga tcgaaaagaa cgaagtacgt atccgagtaa ttttcgctac tccaaaacca
     6481 ggaacagaaa ttgtcgactt tagaggatta agtccattta tcgatgaaga attttcagaa
     6541 gaatggcgcc tattcttgcc aagagaagaa ggaagccaac gcggtgtggt atttggcgaa
     6601 tttcaattag gaaatatgat tgtcggtacg atcgaagaaa cgggcagttt ggtgacagag
     6661 tatcaaaaag gagagttagt ctgcacttat ggacccattc gtgaaacgct gattacaaac
     6721 ggtgttgata attataaact acgtaaactt gatcggttgg atcaatggaa aaatgcagta
     6781 tgttacgatc ctgctcaatt cgctttaggt ggtatccggg aagcacatgt tcgagcaggt
     6841 gatgctgtag caatcttcgg tttaggcgct atcgggcaaa ttgcgattca attagctaaa
     6901 aaagcaggtg ctactaccat cgttgggatc gatcccatag cgcagcgtag agaattagca
     6961 gaaaaatacg gtgccgatta cacgctagat cctatgaaag cagatgtcgg ttttcgtcta
     7021 aaggaattga cagataaacg tggagtagat tcagttattg aaacgagtgg cagtgctgcc
     7081 gcattacaag cttctttaag agggatcggc tatggcggga tcatttctta tgttgccttt
     7141 gctaaacctt ttccagctgg gtttaatctt ggtcgcgaag cacatttcaa taatgcaaaa
     7201 atcgtctttg ctcgagcatc tagtgaacca aacccagatt acccaagatg gaaccgtaaa
     7261 cgaatcgagg acacatgttg ggaattattg atgaacggtt acttggattg tgaagaaatc
     7321 attgatccag ttgtgccatt tgcagaaagc gcagaagctt acatgcgtta tgtagaccag
     7381 cacccagaag aaagtatcaa aatgggcgtt caagtccgag gaggagaaca atgaaattcg
     7441 gtacgcaaga tcaagcattt ttcccaagag acattttaga aaaatttaga tttatcaaag
     7501 actgtggatt tgaagaatac gaaatcgacg gtaagttgtt aatagaaaat atcgacacag
     7561 tcaaacaggc aatcaaagaa acagggatcc cagtagtgac agcttgtaac ggctacgatg
     7621 ggtggatcgg tgactttatc gaagaacggc gtcttaatgg tgtacgtcaa attgctgaga
     7681 ttttgcgtgc actaagcgag gttggaggca aaggaatcgt tatcccagca gcttggggaa
     7741 tgttcactta tcgattaccg ccgatggttt caccccgaag caaagaagga gactataaag
     7801 cagtcagcga atcacttcgt tatttagata aagtagccga agaaacaggt acggttattt
     7861 atttggagcc tttgaaccga taccaagacc atatgatcaa tttgttagcg gatgcgcgtc
     7921 attacattga ctctaatcaa ttgaaacatg tgcagatcat cgctgatttt tatcatatga
     7981 atattgaaga agatgatatt tcaaaagcat tgactacgca aaaagagtat gtgggtcata
     8041 tccatttagc agataatcat cgctaccaac caggatctgg cgcaattgat tttactcgcc
     8101 attttcaaac gctacttcaa aatgagtatg aaggttatat ggtatatgaa tgtcgtttgc
     8161 gaggagaaaa tgaagtgaaa atttatcagg aatcattgca atacttgaaa aactgtctgg
     8221 aaacagcgcg tcatttaaat gatttagaga aagaatagag aaagaataac aataagtaaa
     8281 taagcaaagg agggagaaat aaatgaagaa agtagcaatc ataggcgctg gacaagtagc
     8341 agaaaaagta catgctgctt attatcaaaa aagatcagat gtcttagaat tggccgctgt
     8401 ggttgatcca gtcagccagc gagcagaaga cttttgccga cgtacaggat tttctcatgc
     8461 gtatacatca gttacacaaa tgctgaatga gatttctccc gatatcgtga gtgtctgtac
     8521 acccaatcgc tttcacttcg aacatgtatt ggctgccctc gaagctaatg cttcagtgat
     8581 gtgcgagaaa ccaccagcta tgacagctga tcaagcaaga aagatggcag agacagccga
     8641 taaaaacggg cagattttgg cttatggatt ccagcatcgt ttttctgacg aagcaacaat
     8701 gatcaagaat catcttgatg ttctcgggga tatttattac gtagaagcga atgcgcttcg
     8761 tcggtctggt gttccaggct ggggaacgtt tatcgacaaa gaacttcaag gaggcgggcc
     8821 tttgatcgat tatggcattc acatgcttga tacagcgatg tatattactg gatttcctaa
     8881 agtaaaaagc gttcgggcgt acagcttcca aaaaattggt acgaaaaaaa attcagggaa
     8941 attcgggaaa tgggatccaa gcaaattcac agtagaagat gcaatttttg gcacactgga
     9001 attagaaaac ggcaagatca tccaactgaa tacttcgttt gctttgaata tcagagaaga
     9061 caaactgaat gtggcttttt gtggcgatca ggcaggtgct aatctgtatc caggcgaact
     9121 attcgaagat gtagatggaa agctggtttc cttacaaaag accacgccag ttaatgaccc
     9181 acatgaacta agtatggctc attttgtgaa tcgggcgctc ggtgaccaga ctcagatgat
     9241 tgcagatggc tggcaaggat atgtcatcca gaatctggtg gaaaatttat atcgttcggc
     9301 agaaacagga gaagttatta ccttatgaaa gtagaagaaa aacagtttga tttagagtat
     9361 atcaataagt atgcgagtca gatgaccatt ggaaatggat tttttggtat cagaggaagc
     9421 caggaggaag actatcggaa tcaagtaagg ggaatgtttg cagctggtgt atataaccga
     9481 cctttaggag cagaatcagc cgagttagtg aatctcccag atgtcatcag atgggaaata
     9541 aaaatcgacg gtgaacattt ttctcttgag acgatgaaag ttcttgcata cgagcgggca
     9601 ctagatttag aaatgggaga gcttaaacgc tatgcgctca ttgaaactgc tcaaggaaaa
     9661 aaagtggaag tcattacttg tcgtgttgct gatcaaaata atctccatca ggcagccctg
     9721 aaagtggtca tcaagccgtt gaccgaagac tgccaagtag atgtgaaaac gggtatcgat
     9781 ggacagcaaa ccaattttgg tacacagcag ttggttgaaa aagaactgcg agtatttggt
     9841 gacgatttga tcagtgcatc ctaccagaca acagaaagta aaattgatat tgggattgca
     9901 gctaaattca ataaagcagg tgtattccaa gcaaaaaatc gccggatcac cgcttccttt
     9961 tcagaaaaga tcagaaaaaa tacgtccttt acgctggaga agatcatcgt gctctctacc
    10021 tctcttcagc aaacagaccc gtttaagaaa agtattgccc aagcccagaa aagcgttagt
    10081 tatacagaaa ttttagagca gtcgatgtct tattggacag atttttggca tcagcatcgg
    10141 ataacgatcc aaggcgattc ttctttcgat cagttagcga tggacttcgc tgcttaccat
    10201 ttacagatta tgactccccg caatgatcca agtgttagtg taggggcgaa gggactaact
    10261 ggcgaaggtt ataaagggca tgttttctgg gatacggaaa tttttatcct gcctttcttt
    10321 ttatatcagt ttcctgaagt agcaaagaat ttgctgctgt accgttatga tcgattagaa
    10381 ggcgcacggc aaaaagcaag agattatcac tatcaaggtg cacttttccc atgggaaagc
    10441 gcacacactg gtcaggaaga gacacccaaa tttgctgcat tgaatatcaa aaccggaaca
    10501 agacaagaag ttgcttcagc tgccgcagaa catcatatca gtgcagacct tgcttatgcc
    10561 gttcaagagt atgtccaagc aacagaggat caatcgtttt tagaaacaaa gggaaaaacg
    10621 ctcattcatg aaacagcagc tttttggctg agcagagctg taaaagtcga tgatcattta
    10681 gagatacaca gagtcattgg tccagatgaa tacacagaat atatcgacaa taatgcatat
    10741 acgaactatc tggcgcatta taatgtggcc ttagctgtta aatatcaagc tggcgatccg
    10801 ctttttttga aagaatgtca aaatttttta gataagttat atttgccagt gcctaacaaa
    10861 gaacagctga ttccgcaaga tgacacgtat ctcagtaagc cagttattga tttgaccact
    10921 tacaaagaac gtcctggcag ccagtctatt ttacttgatt atgcccgtag cgaagtaaat
    10981 gaactccaag tcagcaagca agcagatctt gtcatgctgt tatacttatt acctgagtat
    11041 ttttctcaac caatcgtttc taagaatttg cattactatg aaaacaaaac aatccacgac
    11101 tcatcgttga gtaaagcgat tcacgccata gaggcttgcc gtatccaaga aatggaatgg
    11161 gcgtatcatt tattccaaga agcgtgcctg attgatttag gaccagatcc gcattcatca
    11221 gatgacggaa ttcatgctgc ttcattagga gcactatggc tagcagttat atttggtttt
    11281 tcaggtatta cagtaacgaa tggaactctt tctatttctc caagacttcc ttcagcatgg
    11341 gaaaaaattt cttttccttt tatttggaaa aatcacaggt tgctatttgt tttgacaaaa
    11401 aaacaaatag taataaaaaa agagacgaaa gaaacattac ctatttttgt caatggagac
    11461 caataccagc tggaaaaaga actgatcttg gaacggaggg gaaaataatg cgtattctga
    11521 ttgcgccaga ttctttcaaa gaaagtctga cagccttaga ggcagcacaa gccattcaat
    11581 caggttttca agaagttttt gttcaggctg aatatgatct gctacctata ggcgatggag
    11641 gagaaggaac ggtttcatca ttagcagaca gtttaaggtt gaagcggcaa acagcagtcg
    11701 ttactaatgc atttggcaaa aacaaagaaa tccagtacgc ttcagacggt caaactgcga
    11761 tttttgaaat ggcagaaatc gtcggattga aagatgtccc actaaaccaa caacagccac
    11821 ttaggttatc cacgcgagga gtaggagaac tgatacgaca attgttgcaa caaggtgttc
    11881 gacacatcat catcggcgtt ggcggaagta gtaccaacga tggcggcatc ggcatggcag
    11941 aaggtctagg ttatgctttt tacgatcaag ataatcaaaa agtagaagca ctgggagaaa
    12001 atctaggtaa aattgctaca gttgataaaa cgaacgtgcc tattgagctg attaaagcca
    12061 aaatcactgt cattactgat gttactaatc ttttatgcgg aaaagaaggc gcttcttttg
    12121 ttttcggacc acaaaaaggc ttgaaagaaa atgaattagc agtggctgac caagccatgc
    12181 atgattttta tatgagattt gcccctcaag taatcgaggc atcgggcagt agtgctggtg
    12241 gtggaatggg tgcaggactg ttagcatttg cttcagggaa aatagtctct ggcatcgact
    12301 acgtattgga tttactggag ttcgataaac gggtacagca agcagacctc gtaatcgtag
    12361 gcgaaggacg tctagatgga caaagtctaa aaggaaaagc gccagttggg attgctagaa
    12421 gagtaccaga gggcgttcct gtgatagcta tttgcggaag tgttggtaaa ggaagcgaaa
    12481 aagcagcaga tgttggcatt tcggccgttt ttccgatcct tcctgcagct gtgcctttac
    12541 aagaagcttt acagcaagcc actgataatc ttcatcgcac agctaaaaac atcgcatctt
    12601 tacttcaagt cgttcatttt caaggaggta atcgatgaaa atcaaagcag cactattcga
    12661 tttagatggc gttcttgtag acacagctcg ttatcattac gaagcgtggc tggttttagc
    12721 taaccagctg tccattccat tcactgagaa agaaaacgag caactgaaag gaatatccag
    12781 aaccgagtca ctagagcgat tgctgtcatt tggaaaaatg gaacagaaat tctcagaaaa
    12841 agagaaatca gcctttgctg agcaaaaaaa caatctatat cttcaagcga ttcaaaaaat
    12901 ggatgaaaca tccgttttgc ctggtgcaat agctgttctt gagtacttga aaaaaacaaa
    12961 catcaagatc ggattgggat cagcaagcaa aaatgcacgc ctcattttag aaaaaacgaa
    13021 tttgacaagt tattttgatg tccttattga cggcacgcaa gtatcaaaag caaaacctga
    13081 tccagaagtt tttctcaaag gagcacagca attgaatgta ccgccaaatg catgcttggt
    13141 cattgaagat tcagaagctg gttgtcaagc agctttggct ggtaacatgc atgtattggg
    13201 aataggtgaa aacataaatc ttccttctgc tgaatatgtg attcctgatt taaccgtttt
    13261 tgatcaagta cgttcgtttt ggcatttatc agaagccgta tcacgtatat aatagtttcc
    13321 gttttttctg tcacaagcaa aacgttatcc tccttttgga aatcaaggta tgatgagaaa
    13381 aaagggggat tttatatgac aaaaaaagca gttttcgcag gcggatgctt ttggtgtatg
    13441 gtcaaaccat ttgaagagca gccagggatc atttctgtga catccggtta tactggcggg
    13501 catgtaccaa atccaactta tgaacaagta acaacaggca caacagggca tacagaagca
    13561 gtagagatcg aatatgatcc agaagttatt tcttatgatc agttggtgga aatctattgg
    13621 cagcaaaccg atccgactga cgcattagga cagttcgctg atcgcggtga ttcttatcgt
    13681 ccagtgatct actataatga tgaagaagaa cgaaatattg cagagcgttc aaaacaagca
    13741 ttgcaagaca gcgggcgatt cgataagcca atcgtgacaa aaatcgaacc aagaagcgaa
    13801 ttttatcctg cagaagatta ccatcaggat tattacaaga aaaacaagct ccattacaat
    13861 ctttatcgag aaggttctgg acgtgcaggt ttccttcgtc gacattgggg cgtcaagaaa
    13921 taagaagccg aaaaagctac ttatggaaca ccgtatatca gaggttgtaa aaccagcatt
    13981 ttgagctaat actgagggtc agctgattga acaccattta gtggagaggt tgtgaacctg
    14041 tcgcctagct ccaagtcaca aggatttggt aagagaacac tatatatcag aggttgtgac
    14101 aagtcgtttg tcacaacctt ttttcaatac ctaagatgaa ggacaatcag caacgcactg
    14161 ctcaaaaaag gaatgaaagg cagcttttcc aacttttttt tatgacagat aaagtaaaca
    14221 gaaaaaacgg atattccaaa cagactagca agaaacaaga tctgaagcaa ctcttctaga
    14281 gacaagccac cactccaaga aagcatcaac agagtatcgc cagctccgag ataggattga
    14341 ccatagcgaa ggcacacacc gcctacgata aacgttaaca gcacactttg ccattggaag
    14401 acgccggcct gcaaccaaaa aatccataaa attgcccaag aacagaatag cgtgtgagaa
    14461 tggaccatga agtaaaataa atccatcaac gaaaatagca aagccgaaag catccatatg
    14521 aacgtcgaaa aatttcttgc ggaagaaaaa gtaaagaacc aggcaaaaag accaccacat
    14581 aaggcttctg ccaacaaata gtagaagggc aaccgacacc tgcaatagcg gcagcgaaat
    14641 cgttggacga tgatcgagag aatcggaatc aattcatacc aagaaagcga acgacagcat
    14701 ttgacacaat gagatctagg ataaataata gagtgaccta acggaatccg ctgggcggtt
    14761 aaacataaaa atgatccgat acagcaacca atgataaaat aaagtaacac gccatctccc
    14821 ccttttcaaa accaagatac gcaagcttaa actaaatttt tgtacataca taaaatttga
    14881 ggtgaaaaaa tgaacgtttc aatcggatta gatatcggaa ccacacaaac caaagcagtt
    14941 gctttcacag atgaagccca agaagcagca tccgcttatt ttcgctaccc tttgatccaa
    15001 gaaacacaag ggatggcaga acaagatcag gaattgatct ttcaaggtgt ctgcaccgtt
    15061 ataaaagaag taattaaaca gttgccttca gacacaatca ttcaatttgt ctccttctcc
    15121 tctgcgatgc atggcctgat attgttggat gaagagaaaa caccgttatc tcgaatgatc
    15181 acttgggcag ataaccgagc agcggacgaa gcagaaaaac tgaaatatac cgtgcttggc
    15241 aaagaatttt atcggcagac tgggacgccg attcatccaa tgacaccatt gaacaaaatc
    15301 aagtggctgc aaaacagcca acctgaatta ttcaaaaaaa ctgcttattt tgtcggtatc
    15361 aaagactata tcttttatag attgtttggc gaactgatca gcgactacgg aacagcttca
    15421 ggaactggat attttgatat ccatgaattc caatgggcag aagaagtgct tgactacttg
    15481 aacattcgaa tagagcaatt gccgcaagtc tttccttcca cctatcaagt aacaggtcta
    15541 ccatcaaaaa cagcagcaga actaggtttg ccaaatgata tatcatttgt tttgggaggg
    15601 gcagacgggc cgctttctaa tctagggtta ggtgcttttg gtgatacgat cgccgctttg
    15661 accgtaggaa cgagcggagc acttcgtttt attactactc agcctcagtt ccatccagaa
    15721 atggagacgt tttgttatgt tctggatcag aaacattggg tagtgggagg agcgactagc
    15781 aatggtgctg gcatctttga ttgggcaggg catacatttt tacaggaagt cgttcaatct
    15841 gctaaagaaa aaggagaaaa tccatatgat gcactgcttt cggctatcgc ttttgtgcca
    15901 gcgggtgcca atggtctgat ttttcatccc tatttacttg gtgagcgagc accgctttgg
    15961 gacgcagatg cgtttggcag ttttatcggt ttacagcggc aacatacaga aaaagaaatg
    16021 atgaaggcga ttctagaagg gatctgtctc aacctttatc ggattttaaa aggagtttgc
    16081 caggaaaatc agctcacaga gatccgtgct acaggcgggt ttgctcaatc tccgattttt
    16141 cgtcagatga tggctgatgt actgggttac ccgcttgttt tccctactgt cacagaagca
    16201 tccgcattag gagctgtgat tttaggctgg agaagcatcg gaaagatcca aagtatcagt
    16261 gaagcacagg aattgataga attgaaagag caagttggag tacaagaaac tgatcataaa
    16321 aaatatcaga aaatctatcc attatttgtt tcgacacaaa agcatttagc acagacttat
    16381 cagcaatttg ctcaactgcg agaagaatta gctgattctg aaaaatgatt ggctttacgg
    16441 ataaaaaagc gaactgaaaa tcgttctcag gtaaatgatt ttgttgtact tttcattgaa
    16501 agtctactat tattttaagt gtaagaaaat gttaggaggc aagaaagatg aaattccaac
    16561 aaaccaaaga agtattaaat caattagttg cagatctaag ccaaatgtct gttgttgttc
    16621 accaaacaca ttggtacatg agaggaccag gtttccttac attgcaccca atgatggatg
    16681 agttcatgga tgatttgaac gctcagttag atgaagtatc cgaacgcttg atcacattag
    16741 acggttcacc ttattctaca ttgcgtgaat ttgcagataa cacaaaaatc cctgatgaaa
    16801 ttggccgctg ggatcgtact attccagaac gtttggaaac acttgttgca ggctatcgtt
    16861 atctagcaga tctttaccaa aaaggaatcg aagtttctgg agaagaaggc gacgatccaa
    16921 cacaagatat ctttatcgaa ttcaaaacag ccacagaaaa acgtatctgg atgatccaag
    16981 ctcatttggg caaagcgcca gagatcgacg cgtaaaaact gttgttgaac ttgaccgaaa
    17041 aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgt caaaatagtt
    17101 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttac
    17161 caagaggttg taagcctgcc gttcagctcc gagtcacaag aaacaatttt aaaaaacagc
    17221 ttcttatgtt ttatggatat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    17281 attcaaaata aggggttgtg acaagacttt tgtcacagcc tctttttgtt tttcattttg
    17341 acaggaaacg acccttttat ataaaagaaa gaaaagaagt aaagtttcat ataaattttt
    17401 caatcaagca gaaaaacgtt gacattgact agattagcgg gtaatatgtt aaatggtatt
    17461 gtttgcccca caaagagccc cggaaactcg aagtgtatgt caaacatcag aacttaaatt
    17521 ggaggaaaat aacgtgcgta caacatatat ggccaaacca ggcgaagtag aacgtaaatg
    17581 gtatgtagta gacgcaactg atgttccatt gggacgtctt tcaacagttg ttgcatctgt
    17641 actacgtggt aaaaataaac caactttcac acctcatttg gatactggcg atttcgtcat
    17701 cgtcatcaat gcagacaaag tgaaattaac aggtaaaaaa gcgactgaca aaatttatta
    17761 ccgtcacagc aactacccag gaggattgaa atcaatcact gctggcgaat tgcgtgctaa
    17821 aaattctcgt cgtttgatcg aaacttctgt aaaaggtatg cttcctaaaa acactttagg
    17881 ccgcaaacaa tttacaaaat tgaacgtata cggtggagca gaacatccac atgcagcaca
    17941 acaaccagaa gttttagaca tcacaaactt aatctaatag gagggaattt cattggcaca
    18001 agttcaatat atcggcacag gccgtcgtaa aaacgcagtt gctcgcgtac gtctagtacc
    18061 aggaactggc aaaattactg taaataaaaa agatgttgaa gaatacattc cacatgctga
    18121 cttgcgtgaa gtaatcaatc aacctttcgc tgttactgaa acaaaaggcg catatgacgt
    18181 atttgttaac gtaaacggcg gaggatacgc tggacaatca ggagctatcc gtcatggtat
    18241 cgctcgtgca ttgctagaag ttgatcctga cttccgttta gctcttaaac gtgctggctt
    18301 acttactcgt gacgcacgta tggtcgaacg taaaaaacca ggtcttaaga aagcacgtaa
    18361 agcttcacaa ttctcaaaac gttaatatcc tttatatatc aacgtttctg gcacttttca
    18421 attatttgga aagtgcttct tttgttttta agaacacatt taagaacaca tatcaatcac
    18481 acttattcaa tcctctctga aaagcttcaa aaacagcctt atcaactata tctggtgtgt
    18541 tcacatatat ctttgttgta gaaatatctg agtgtgttaa agcatttgaa atttgattca
    18601 tatctgctcc accttgtcta gctatagttg cataagtatg acggagtttg tgtggagata
    18661 aatgaactaa gtcaggatgc cttcttttaa ttgaattgat tcgatagttt aggtaatcgg
    18721 catgaacagg acaatttact tctccagatg gtttagtata agtaaagaga tactgatcta
    18781 acgtttgctt aatttttaat ttcaacaatt gatcagcttg taccgatttc cattcactta
    18841 gcaatgtcat tacaacttct ggtacttgaa tgacggtatc ttttcgacct tttgtaaact
    18901 tttttctttg atatttatct aaagtatgtt taagacggac cgtttgtttt tcaaaatcaa
    18961 tatatttcca ttgcaaagcg taagtctcac tttttctatc accaagatac agggtaatca
    19021 taaaaagtgt gtaatcatga aaagtaagta aatgattatt aaaatcatca tttactgctt
    19081 ctatccaatt agtaagttct tttgatgata gtgcttgctt accttcttgt attctttttt
    19141 cttctaaagc atttttttta ggtgcagtga tactttgaat aattttagta gttcgattaa
    19201 attcgatata atttagtatt tctgcaatat cgaacatgga acgaacatat ccttttatga
    19261 tttttatatt tgcatattct ttcgattttt tggttaattc atctgatatt atctcagtat
    19321 tgatatttag ataattcatt gcgtattcac caaacatagg taaaatatgc aaccgaaata
    19381 tatcttttgt attactaatg gttatatcac taggaggcct gtttgatcta attgtttgac
    19441 ctaattcata tctaggaagc catttagatt cgtaaaattt tttaaacgtt atttttcctt
    19501 ttaattctaa tgagtttgca ttctcctctc tcaaaacttt ttctattttc ttcttcatat
    19561 cattttcggc tttaatcgcc aattgcctat ttggaaatgt tttttgatac ttttctgatg
    19621 caattcctgt tatttcttta aacctttttg gatatctcag attagtttta tatttcccat
    19681 ttgaaagctt tgtaggcaaa atcctcactc cttaaaaaaa gtaaggcagt atatttcact
    19741 aattttagta tatgcggcga gaagaaaatt tcaa
//
LOCUS       DAZUKD010000049        19286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000049 DAZUKD010000000
VERSION     DAZUKD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.050"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..946
                     /locus_tag="ACG8N9_001482"
     CDS             257..946
                     /locus_tag="ACG8N9_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4897087.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            933..2141
                     /locus_tag="ACG8N9_001483"
     CDS             933..2141
                     /locus_tag="ACG8N9_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4897088.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2435..3706
                     /gene="serS"
                     /locus_tag="ACG8N9_001484"
     CDS             2435..3706
                     /gene="serS"
                     /locus_tag="ACG8N9_001484"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4897089.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4255..5739)
                     /gene="guaB"
                     /locus_tag="ACG8N9_001485"
     CDS             complement(4255..5739)
                     /gene="guaB"
                     /locus_tag="ACG8N9_001485"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4897090.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6244..6939)
                     /locus_tag="ACG8N9_001486"
     CDS             complement(6244..6939)
                     /locus_tag="ACG8N9_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4897091.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7029..8129)
                     /gene="ychF"
                     /locus_tag="ACG8N9_001487"
     CDS             complement(7029..8129)
                     /gene="ychF"
                     /locus_tag="ACG8N9_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4897092.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8138..8329)
                     /locus_tag="ACG8N9_001488"
     CDS             complement(8138..8329)
                     /locus_tag="ACG8N9_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4897093.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8350..9240)
                     /locus_tag="ACG8N9_001489"
     CDS             complement(8350..9240)
                     /locus_tag="ACG8N9_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4897094.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9227..9994)
                     /locus_tag="ACG8N9_001490"
     CDS             complement(9227..9994)
                     /locus_tag="ACG8N9_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4897095.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10006..10719)
                     /gene="rsmG"
                     /locus_tag="ACG8N9_001491"
     CDS             complement(10006..10719)
                     /gene="rsmG"
                     /locus_tag="ACG8N9_001491"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4897096.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10951..12852)
                     /gene="mnmG"
                     /locus_tag="ACG8N9_001492"
     CDS             complement(10951..12852)
                     /gene="mnmG"
                     /locus_tag="ACG8N9_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4897097.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12869..14266)
                     /gene="mnmE"
                     /locus_tag="ACG8N9_001493"
     CDS             complement(12869..14266)
                     /gene="mnmE"
                     /locus_tag="ACG8N9_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4897098.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14565..14948
                     /locus_tag="ACG8N9_001494"
     CDS             14565..14948
                     /locus_tag="ACG8N9_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897099.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15116..15874)
                     /gene="jag"
                     /locus_tag="ACG8N9_001495"
     CDS             complement(15116..15874)
                     /gene="jag"
                     /locus_tag="ACG8N9_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4897100.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15892..16710)
                     /locus_tag="ACG8N9_001496"
     CDS             complement(15892..16710)
                     /locus_tag="ACG8N9_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4897101.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16707..17063)
                     /gene="rnpA"
                     /locus_tag="ACG8N9_001497"
     CDS             complement(16707..17063)
                     /gene="rnpA"
                     /locus_tag="ACG8N9_001497"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4897102.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17181..17315)
                     /gene="rpmH"
                     /locus_tag="ACG8N9_001498"
     CDS             complement(17181..17315)
                     /gene="rpmH"
                     /locus_tag="ACG8N9_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4897103.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17838..19172
                     /gene="dnaA"
                     /locus_tag="ACG8N9_001499"
     CDS             17838..19172
                     /gene="dnaA"
                     /locus_tag="ACG8N9_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4897104.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         5460 a         3970 c         3562 g         6294 t
ORIGIN      
        1 aataagaaaa tgcttattta tcaacatttc tagcttacat gatcattggt tagttctggt
       61 cctttttcgt tcgtctttat aactccgaga gacgtaaaat ccttttgttt tcagtttaat
      121 gttcatgttc ttgtcttatt cacaccaact agatgtctca ccttctaaaa ttttccttaa
      181 caaagtgctg cttttatcta tttctgcttt tttttgctag aataatagta acaatatgac
      241 tctggaggat ttatttatga aaattttgat tgctgacgat gataaagaaa tcgttgaatt
      301 attaagtatc tatgttcaca atgaggggta tgaagcagta aaggcttatg atgggaaaga
      361 agcattatct aaaatcagaa caatccctga tattgaatta cttattttag atatcatgat
      421 gcctgagatg gatgggatgc aagtagtgaa agagctacga aaagaatccc aaataccaat
      481 cattatgctg acagcaaaaa ctactgatat ggataagatc aaaggactgg tcgctggtgc
      541 agatgattat gtgaccaagc catttaatcc gttagaagta atggctagag taaagtcttt
      601 attacggcgt acaaaaatgc agattgcccc agatcagcct gatgtattag aagtaaatgc
      661 attaatcatc aataaagact ctcatgaagt taaaacaatc gatgggaaag aaatccagtt
      721 aacagctttg gaattcggta tcctctactt gcttgctagt catcctaatc gagtatttag
      781 cgcggatgaa atttttgagc gtgtttggaa acaagaaagt attgtctctg ctaaaactgt
      841 catggttcac gttagtcatt taagagataa gattgaagaa gcaactggtg gtgaaaaagt
      901 cattcaaacc gtttggggag tgggctataa aattgacgca agatagtaaa cgagcaactg
      961 aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag cgaattgtta gcggaaggaa
     1021 tcatcacgat tatccttttg cttctattaa atgtttctat tctggttgtt atcagttcgg
     1081 tgatcaacag ttcaccttct ttgacgaatg cgatttggga ttcaaagaat atttttgcag
     1141 aacgattgaa cactgatttg ttctggaacg gccggaattt tattattccc tttttctttc
     1201 tattagatat tggtgttttg tattggcgct tgattcgaag atatcgccaa atgcaattgc
     1261 ggcatatcat cagcgaactt cactacatcg caaacggaaa ttatgatcat cgtatccctt
     1321 ttgaactgag tggtgactta agccgagtgg taacaagtat caatggattg gtggatagta
     1381 ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc aaaagatgaa ttgatcacga
     1441 atgtcagtca tgatatccgt accccactca cgtccatcat tggataccta ggactgatag
     1501 aagatggcca gtatcactca gaagaagatc ttttaaagta tacgcatact gcttatatca
     1561 aagcaaagca gatgaaatct ttagtggacg acttatttga atacactaag gtacgccagc
     1621 ctgctgtccc ggtgaatttt tctgcctttg acatgatcca gttgatcgaa cagttagcag
     1681 ctgattttga gttggaagct tctaaaaaaa atatccaaat acttgttcaa tcaaaagtag
     1741 actcccttat catggatggc gataccgaaa aacttgtacg tgtcttcaat aatcttttga
     1801 cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt aatcgaagta gaacgtatcg
     1861 gttcagaagt tgttgcaaca gtcaaaaata acggagcaat gatcccacaa caggcaatcg
     1921 ataatctatt cgaccgtttt tatcgtgtag aagaatctcg ttcacaagca actggaggaa
     1981 ctggacttgg tttagcaatc gctcaaagca ttgtggcttt gcacggcgga tacatttatg
     2041 caaaatcaga taagcaatgg acttctttca tcatgcacct tccaatcaag aaaaatgaaa
     2101 aattacctat tgatacacag gaagttattg acgaaagctg aaatattcgc tattcttatt
     2161 acaattatta gaataaatga aaaacacaga acccagtaca ctttattctt tttcagagag
     2221 ctgatggcag gtgtaaatca gcaaaggata atttggaagt tccagttcgt ttacgtgccc
     2281 tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt tcaacaagtg cagctgacgc
     2341 tgaacttggg tggtaccgcg aatttcagac tgattcgtcc caagaataat gggatggatc
     2401 agtctttttt tattttcaaa aaggaggaaa tcatatgtta gatgtaaaaa tgatgcgaca
     2461 aaatattgaa gaagtacaag aaaaattgat gactcggggc gtaaaaaaag aaacattagc
     2521 tgaattcatg gaactagatg aaaatcgtcg taagctacta gtcaaaagtg aagaattaaa
     2581 aaaatatcga aatgatgttt ctgctgagat cgcccaattg aagcgtgcaa aagaagatgc
     2641 ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac atcaaagcat tggatactga
     2701 aattgctgaa gtggatgaaa aattgaaaca aattgcaact actttgccaa accttcccca
     2761 tgattctgtg ccagttggaa cagatgaaga ggacaatgta gaaatccgtc gttggagcga
     2821 accacgtaca tttgcttttg aaccaaaacc acactgggaa attgcagaaa atcttgatat
     2881 tttagatttt gaacgtggcg caaaagtagc cggtagccgt tttgtctatt acaaaggatt
     2941 aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt gatcagcata tttacgaaca
     3001 cggctatact gaaatgatca ctccttatat cgtcaacagt aaagcgatgt ttggtacggg
     3061 acaatttcca aaattcaaag aagatgtttt ccaattggag aatactgatc taacattaat
     3121 cccaacagct gaagtaccat taactaacta ttataatgat gaaattttag atggtgcaga
     3181 tttacctatt tatttcactg cactaagccc tgcttttcgt tctgaagccg gaagtgctgg
     3241 tcgggataca cgaggactga ttcgtcttca ccaattcaat aaagtagaaa tggtcaaatt
     3301 ttcggatgta gagcattctt acgaagaatt ggaaaaaatg acagctgatg cagaagatat
     3361 cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta tcaactggag acatgggctt
     3421 ttctgcagct aaaacttatg acttagaagt atggatacct gcacaagata cttatcgtga
     3481 aatcagttct tgttctaact gtgaagattt ccaagcacgc cgcgcaatga tccgttatcg
     3541 cgataatgat ggaaaaatcc actatactca tactctgaat ggttcaggac ttgcagttgg
     3601 ccgtacagta gctgccattt tggaaaacta ccaaaatgaa gatggttctg tcacagttcc
     3661 agaagcgctc gttccttata tggggaatct gaaagttatt aaataaagag gctgtgaaaa
     3721 agccgcttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca
     3781 taaattggac ttaataccga gaaactggct tttgaacacc atttatcgag aggttgtgaa
     3841 atcagcgaat taacctgagt attagagaaa aaaatggaaa aatagtccct catattttac
     3901 cgtttgttga actaataccg agggtcagct gattgaacac cgtttagtgg agaggtcgta
     3961 agcctacccc ccagctccta gttacaagga caaattcaaa aaacagctct tcatatttta
     4021 tgaatgttgt acttgtgatt cggaggtggc aagggaacac tgtttattca aaaagaggct
     4081 gggacagaag cgtttaactc caagaaataa gaagaaattc acgaaaattg cttttcaaat
     4141 ttttgtgaat ttcttcttat ttccgaagga gttgcttcta cttccgccgt ttatacgtgt
     4201 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttaat gtttttaaga
     4261 ttcaactgaa tagtttggtg cttcttttgt gatttgtaca tcatgagggt gtgattcttt
     4321 taatccactg ccgctcattt ggataaattg cgcttcatca cgtaattgct gtagattggc
     4381 tgccccaaca taacccattc ctgaacgcaa accgccgatc atttggaaaa taatatctgc
     4441 aacacttcct ttataagcaa cccgaccttc gattccttct ggtactaatt tatttgcttc
     4501 attgacacta ccttggaagt aacggtcttt tgaacctttt tccatcgcgc ctaatgagcc
     4561 catacctcgg tatgttttga atcgacgtcc ttggaagatt tcgaattcac ctggtgattc
     4621 atctgtccct gccaacatac ttcctagcat cactgcatgt ccgccggcag ctaatgcttt
     4681 gacgatgtca ccagaatatt tgatccctcc gtctgcaata atcgctttgc catattgacg
     4741 agcaactgat gctgcatcat aaatagctgt gagctgaggt acacctacac cggctaccac
     4801 acgtgtcgta caaatcgatc ctggtccgat accaactttt acgacatcta cacctgcatc
     4861 atataaagct tttgtcgctt cagccgtcgc aacgtttcct gcaatcaatg ttgcatctgc
     4921 aaaagttgaa cggatttctt gaatttttcg gatcacacct gcactatgtc catgagctgt
     4981 atcgatgatg attgcatctg cccctgcttc taataaagca ttcgcacgct caaatgtatc
     5041 gcttgttaca ccaacggctg ctgctacaag caatcgacca tgttcatctt ttgctgcatt
     5101 tggaaattcg atcactttct cgatgtcttt gatcgtgatc aatccgctta aacgaccttc
     5161 gttatctacg attggtaatt tttcgatttt atgtttttgc aaaatctttt ctgcatcttt
     5221 tagagaggta cctactgggg cagtcactaa gttgtctttt gtcatcacat cagcaatcgg
     5281 catgtgataa tctgttacaa aacgcatgtc gcggtttgta attatcccta ccaattttct
     5341 gttttcaagt gtttcaacga ttggcacacc actgatacga tatttgctca tcaattcttc
     5401 tgcgtctgct acaagatgct gtggcgttaa gaagaacgga tcaatgatta cgccactttc
     5461 agaacgtttg acttttctta cttcatccgc ttgctggctg atcgtcatgt ttttatggat
     5521 cacacctagt ccgccttgtc ttgccatagc aatggccatc ttgctgtcgg taacagtatc
     5581 catacttgca ctgatgattg gaatattcaa cttaatattt ttagctaatt gtacgctcat
     5641 atccacttcg tttggtaaca cgtgactttc tgctggtatc aataagacat catcaaaagt
     5701 aaatcctttt ttcgtgaact ttgtttccca gttggacatt tttagtacca ctcctctaat
     5761 ttagtttatc tattaaaata attgattttt tcatttaagt caatgatcta cttggaatca
     5821 agttagcttt ctcatagaat accaatataa agcgtttaca tctgtgtgta ttaaaagaga
     5881 ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat
     5941 gtttttgaat tggttgggtt tattaccgca gatgcaaact tttgaacacc gtttagtgga
     6001 gaggctgtga aaaagctgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt
     6061 ccacattttt gcataaattg gacttaatac cgagaaactg gcttttgaac accgtttatc
     6121 gtaaaaaggg actgtgacaa gacttttgtc acagtccctt actattattt aactatggat
     6181 aaacaaaatg aggttcttta ctacccctag atatctagca aaatagctga tctttttgct
     6241 agtttatctt cttgggacgg ataaacttcc aataatcccg tattttttct tcaagtagta
     6301 acggattgct aaagcagttg ccccgataat gataactact actggatcta atactggatt
     6361 gataactggt ggcaatagtg ttgtcgcaga aaacagtacg acccaaagaa gtgtcgttcc
     6421 acctaggata agcaatgtct tacccattcc tggtttcttg cttcgatctg ctccgggata
     6481 ttcatattga tagatgtatt tgtacatcat ataaaagacc cagccaccga ttgcagaacc
     6541 tagaacgagt gtagaaaagc cataagctgg cgttgttcca cgtgaaaaca atccggtaat
     6601 cgcaatcatg atacttaata gaccaagcaa cagcaacgta ttatctaacc acatcaataa
     6661 aggggtggat gaatttttta cttctgctgg cttattgatg attgcttccg ttctttcaga
     6721 gactgtccca aacaattgac gggctgttct tccgcctttt tgttctgcaa ctaaagtagg
     6781 cagaatgtca tgaagggctt ttactttttc ttcttcagac aaattcgctg cttctaaaga
     6841 ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt aattttgttt ctaaactatt
     6901 gttttctttt acgattgctc gtaattgttc tggttccatt atctaatagt cctccttagc
     6961 cgttcctttt ctcaacggtc gtcacaaaag acattctacc atattctaca gctagtttga
     7021 tagccctttt acacattaaa gcggaataac attacgtcgc cgtcttgtac gacataatct
     7081 tttccttcta atcgaacacg tccagcttct tttgctgcat gcatgtttcc gtaatgatcc
     7141 aagtcttcaa aagagacagt ttctgcacgg ataaatccgc gttcgaaatc tgaatggatg
     7201 attcctgctg cttgtggtgc tttgattcct tttttgaatg tccaagcacg tacttcttgt
     7261 tccccagcag taaagtaagt agctaagcct aacaaatcat aagcagcacg gatcaactga
     7321 tccaaacctg attcttcgat tcctaaagct tctaaaaatt ccgctttgtc ttcttcatct
     7381 aattctgcga tttcttcttc tgcacgagca ctgaccacga tcacttctgc attttcttct
     7441 gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt ctgcatctgc tacgtcatct
     7501 tctgagacat tggctacata aagaattggt tttgtcgtca ataaaaatag tgatttaacg
     7561 attttttgtt cttcttcagt aaattcgatc gttcgggcag attttccttc ttccagtaca
     7621 ggtttgattt tatccagtac agctagttct gccactgcat ctttatcttt ggttttagca
     7681 acttttgcta cacgagtata gcgtttgttg atagattcta agtctgctaa cactagttcc
     7741 aagttgatcg tatcaatatc ttctagtgga tcaacacgtc cttctacgtg agtaatatta
     7801 tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta cttggcggat atggcttaag
     7861 aattgatttc ccaaaccttc tcctttactt gcgcctttta cgattccagc aatatctgta
     7921 aattcaaaag tcgtcggaac agtttttttc ggatgtacaa gttctgttaa gcgttgcaaa
     7981 cgccagtcag gtacttctac catccctacg ttggggtcga tcgtcgcaaa gggatagttt
     8041 gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag ttgattttcc tacgttgggc
     8101 aacccaacaa ttccagcagt caatgccata aatttattca ctctttctct gcattttttt
     8161 caattacttt ttttaatttc ttttcaaaat ctcgacgggt catcatgacg atatgtccgc
     8221 aattcataca tctgattttg atatctgcgc ccattcgaat gacttcccag cgattcgtct
     8281 ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa gtcgtacata aaaatagcct
     8341 cctttttgtt tattcttcat caaattgaat attcaagata tcaagaattc gagtcaaatc
     8401 tttttgagaa agatattcaa tctcgatctt acctttccca tttttttctt ggatagctac
     8461 acttgtacca aatttatcca tcaaacgatc ttcgctttct cggatataat atggtttttc
     8521 ctttgctagc cttgggattt ttttcttttc ttttccatca ttttgattta actcagaaac
     8581 aagctgttcc agttggcgga cagttaaatt ttcagttact gcacgattgg ctaatttcaa
     8641 gatcaatgtt ttatttttta aacctaacag cgtacgggct tgtcccatag ataaacgctg
     8701 atcttgtacc atttccttga caagatccgg taaagaaagc aagcgcaaat aattggcgat
     8761 ataaggtctg ctctttccta aacgactagc tacttctgct tgtgttagtt tcaggttttt
     8821 catcagcatc tcatatgctt ctgcttcttc taacggattc agatcttctc gctgcaagtt
     8881 ttccagcact gctacttgca tcatcgcttc ttcgtcaaag tctctaacga tagcagggat
     8941 cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc tcccctgcaa taatctcata
     9001 tccttttact gcggaacggc gtacgatgat gggttgaaag acacctgatt gttcaataga
     9061 attagctagt tcctgcaaag atacttcttc aaatgtttta cggggctgat acggatttgg
     9121 tcgcagctca ttcaatggta gctggacaac ggtatcatct tttacatcca ccgtctctaa
     9181 gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct aatcctttac ttttcttcac
     9241 gacttaccac ttcctttgcc aatgcttggt agacttcagc tcctcgagaa cgtggatcgt
     9301 agtcgatgat cggttttcca tgactaggtg cttctgataa acgaatattt cttggaatga
     9361 tcgtttcgta aactttttcg cggaagtatt tacgtacttc ttctacgact tcatttccca
     9421 gatttgttcg tgcatcatac attgtcagta agactccctc gatctctaat tctggattaa
     9481 agtgtttttg gactaaacga accgtattca gtaattggct taatccttcc aatgcgtaat
     9541 actcacactg aacaggtatc aaaatcgaat cacttgctgt aaatgaattg attgtcagat
     9601 ggccaagaga aggcgggcaa tcgatcaaaa tataatcata ctgactgctt acttctgcta
     9661 aagatccttt taatcttgat tctcttgcca tcattgacgt caattcgatc tctgctcctg
     9721 ctaattggag agtcgcaggc acaatactca aattttcgtg ttcagtgatc aaagttgcct
     9781 catcaattgg caattcattg actagaacat catagatgtc tcttgtcaca tcaggttttc
     9841 tgatcccgac accactggta gcatttcctt gtgcatccat atccactagc aatacttttt
     9901 tgccaaggct agcaagacaa gcacctagat tcaccgtcgt cgttgttttg ccgacgccac
     9961 ctttttggtt cgcaacagat attatttgtg ccatctcttc tcctcctatt tgattggctg
    10021 cttattaggc agtccgggct ttcttgggta tttctttggg gttggtttct ttttttgaat
    10081 aacgatgata tgccgttgat cagagacatt cggcaaggta accgcctctt ctttgaggaa
    10141 tttaccacct aataaagcaa tcgcttgttt tgcttctgtc aattcctctt cactttttgc
    10201 tgctttcaat gcataaaaat agccttcttg tttagccaag ggcaaacaca attccgctaa
    10261 cacattcaaa cgagcaaccg cacgagcagt gacaaggtca aatgatgcac gaaagtgagg
    10321 attttgccca aatgtttctg cccggtcatg atataaggaa gcatcgattc ctaataatcg
    10381 ggttaactca gataagaagg ttattcgttt attcagagaa tcaacgatcg ttatctgcag
    10441 ttctggaaag gcaattttta aaggtatgct aggaaaccct gcccccgaac caacatcgca
    10501 taaagataaa ggttgcgtaa agtctacttc aaatgctaat gtaagtgaat cgtaaaaatg
    10561 cttcagatat acgtcttttt catccgtaat agctgtaagg ttcatctttt cgttccattc
    10621 gactaatgtc cgataataaa tagcgaattg attcatttga tgatcggtaa gctctatccc
    10681 tttttctgcc aaggattggc gaaattcttc tggtatcata aatattcctt tcattgtgaa
    10741 acagcaattg tttcacagat tctctcccta ctttaacgca aaatctctag acatgcaatc
    10801 cttccatatg gattcttaaa aatttgaaaa gtaagaaaag ccgatagact ttttctttct
    10861 agtatgactc ataaaatata cacagaagag agaactagct ttgagcaatt tctgcaaaaa
    10921 ctagttctct cttctattta ttattacgta ttacgctggt tcactgactt tcgcaatctt
    10981 cccttgttcg atatagacca ttaggatact taggtctgca gggtttacgc cactgattcg
    11041 gctagcttgg gcaattgttt ctggttggat tttctgcaat ttttgttttg cttctgttgc
    11101 taatccgttg attgctgcgt agtcgatgtt ttctggaatc tttttcgctt ccatgcgttt
    11161 taatttttct actttttcca tggctttttt gatgtagcct tcatatttga tttggatttc
    11221 tacttgttcg atttcttttc gtgtcaattc ttcattttct ggaataaacc gtagaagttc
    11281 tgcataagta atctcaggac gtcttaaaaa gtcgcttgct agtacaccat cttttaatgg
    11341 tgccgcatta atcgttgcta aaaagtcttg gacttcttct gttggtttga ttcgtacatt
    11401 catcaaacgt ttgatttctt gttcaactgc ttcttttttc ttcaaataat tcttgtattg
    11461 atcttctttt actaagccta cacggtgacc catttctgtt aatcgcaaat ctgcattatc
    11521 atgacgtaaa agcagacggt attcagcacg tgaagtcagc aaacgataag gttcgttcgt
    11581 tccttttgtt accaaatcat cgatcatgac accgatgtaa ccgtcacttc gtttgatcac
    11641 taaaggttct tttccctgaa ctttcaaagc tgcattgata ccggcaatca gcccttgtcc
    11701 agccgcttct tcataaccgc ttgtaccatt tgtttgtcca gctgtataaa gattttcaac
    11761 gactttagtt tctaatgtcg gacggagctg gtgtggctct actacgtcat attcgatagc
    11821 atatcctgta cgcatcattt ctactttttc taatccagca atagaatgaa gcatgttgac
    11881 ttgaacatcc tctggtaaag aagtagataa accttgaaca tagacttctt ccgtatttaa
    11941 gccttctggt tccaaaaata attgatgacg tggtttgtct gcaaaacgca cgatcttatc
    12001 ttcgatggat ggacaatagc gagcgcctac cccttcaacg atccctgtaa acattggggc
    12061 acgatgtaag ttgtctcgaa taatttggtg agtcgtctca cctgtatagg tcagccagca
    12121 agattcctga tctagtttat aggcactatc tggtgtgctg aagctaaaat gattcggctc
    12181 tttgtcacca ggctgctctt ctgtaacaga atagtcgatc gtacttgatt ttactcgagg
    12241 cggtgttcct gttttgaacc gttcgatagc aaagccaagt tcttttagat gattcgctag
    12301 tccaatagaa ggttgagagt tattcggacc tgatgaatat ttcaattcac cgataatgat
    12361 ttctccgcga agtgctgttc cggctgtaat aacaactgct ttggcccggt agctcgctcc
    12421 tgtggacgtc actactccat gacaaacacc atcttcgacg atcagttctt cgacgattcc
    12481 ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt ttcatttctg ttgaataagc
    12541 atgtttgtct gcttgtgctc gcaatgctcg gacagctggg ccttttcctg tgttcaacat
    12601 acgcatttgg atataggttt tatcgatatt cttacccatt tctccgccta acgcatcgat
    12661 ttctcgaacg acaacgcctt tagctggtcc gcctacagaa ggattacatg gcatgaatgc
    12721 taccatgtcc aaatttattg tcagtaataa tgtttttgat cccatacgtg cagcagcaag
    12781 tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc acgtcatacg catctgcttc
    12841 ataatgttcc aatcgtttgt tcctcctttt attttcctaa acaaaattga ctgaataatt
    12901 gagtgattag ctcatcttgt acactgtcgc cgacaacttc tcctagatag tcccagcaac
    12961 gcgtcatatc tatttgaacg agatccacag gcattcctga atcaatacct gcaatgactt
    13021 cttcaagtga gagggaagct ttttctaaca acgcgatatg gcgggtatta gaaacataag
    13081 tagcgtctct ttctcctgtt tctccgctga agaataaatc agaaatagct gattccaaac
    13141 gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac tggttcattt tcgatcaatt
    13201 tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt tttatttaga agaatgatcc
    13261 gtttcaatcc acttgtcgct tccaataatt gttcatcttc tgctgtcagt ggttcgcttt
    13321 ggtttagaac aagtaagatc aagtctgctt ctgctaatgc ttttcggctt ctttcgaccc
    13381 cgatccgttc caccacatct tctgtttccc gaattccagc tgtatcgatt agcttgagcg
    13441 gtaccccccg aacattgaca tactcttcga ttacatctcg agtcgttcct gcaatatccg
    13501 taacgattgc tttttcttct cttaacaaat ggtttaacaa acttgatttg ccgacatttg
    13561 gccggccgat gattgccgta cttaaccctt cccgcagaac cttcccttgc ttggatgtag
    13621 caagtagcgc ttggattctt tgttggatca tctgcgcttt ttcaagcaat aacttcgtag
    13681 tcaattcttc gacatcatca tattctggat aatcaatatt tacttctact tgtgctaatg
    13741 tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga cagattgccg tctaactgat
    13801 taagagctag tcccattgct ttgtctgttt ttgcccgaat caaatccatc acagcttctg
    13861 cctgtgaaag gtcaacacga ccattcaaga atgctcgttt tgtgaattcg cctggctcag
    13921 ctaatcttgc accttctctt aacaatagct gcaaaatttg attgactaca acgatcccac
    13981 cgtgacaatt gatttctact acatcttccc gcgtaaatgt ctttggtgct ctcatcacgg
    14041 agaccatgac ttcatcgaca agctgattgc tcttgggatc aacgatatgt ccataatgga
    14101 tcgtatgact tggtacttct gataatcgtt tgtttccaga ttgataaact ttatctgcta
    14161 gttgcacagc ttgatcgcca cttaaacgaa cgatactgat tgcaccttct ccaggtgggg
    14221 tagaaattgc cgcaatcgta tcaaattcta aagtaatttc agccattttt tcacttcctt
    14281 tttcattttg cgcacaaaaa aagtgcccac tccacccttt atgcacaata aacggtgaac
    14341 ttcgcacttt gactgcttgt tcttgattaa attctggtca tagattatca cacttgctat
    14401 aaggaatcaa gtgtttaaaa caattagatg aagttgagcc tgttctgctt ccattacctt
    14461 atatttaatt gtccttggat gtctctttgc gttctttggt ttattcgaag agaagatact
    14521 gtataaacaa ggctttatct ttcttaaaaa aaacggtaca ctaaatggta gcgctatcta
    14581 aactatacac ttttgaaagg agaaaatatg tgagcccgtt cagatctgct gcattggaag
    14641 aagaaagtga ccctcggatc aacgatgtcg ttttatctac agggaatatc aaccagaaat
    14701 atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga agctgatttg tttgacccat
    14761 cttttcccaa agatgacctt ttcttaacaa cgaagaggaa attaaagacg aaggtacttg
    14821 aatatggtgc gaatgcagta atcaactgtc attttgagta tcagcaaaaa aaacgcgacg
    14881 gaaagtattt tctggaactt tttgcttatg gaacagtgat ccaatttatc caaacaacga
    14941 ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg aagacagttt cgatccgctt
    15001 ttttctatca aagttagtga ctacttcatc gtatactctg ataagaatga tttctagtaa
    15061 aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt caaaaagtaa tggaatcaat
    15121 aatatttttt agcaggttct actactaaat aacgataagg ttcctcacct tccgaatgtg
    15181 tttttacatg ttcatctttg cttaatgcag catggatttg tttacgttca aatgcaggca
    15241 ttggttctaa gaaaacaggc tgccctgtcc gttttacttt ttcagccgtg cgatcagcta
    15301 aacgctgaat gatctcttgg cgcttttcac ggtaattgcc tacattgact actacagaca
    15361 gcttatttct tgctaaacga tggatgaaga cttgtgccag atattgcaat gcattcagtg
    15421 tttttccatg tttcccaatc agtatgcctt gcttttgtgt ttctaaatgg aaaacaacca
    15481 tattatttga ttctctttcc attttgacaa gagctggagc atcaagctgt tttgaaattt
    15541 cagtaagata aactgccaat tccgttaaag ctgcttcatc acctagctca cttacttctt
    15601 ctgttttttc ttcaagaatc gtttcgttga ctacctcgat ctcagctgtt ggttcttctt
    15661 ccaaaatttc ttctacagtc tgttctactg tttcagaaac tttttcgctg atacttggct
    15721 ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa accttttttt ccttcttcca
    15781 gtatttcgat ttcgacttgg tctttggcca aacccaaatc agccagacct agttggacag
    15841 cttcgtctat tgttgcagct tcataaatcg gcattagcat tacctcccct attatttccg
    15901 ttttttctta ggattcattg ctttcttaag agcacgttct ctttcgcgga ttctccgtgc
    15961 ttcttcttca cgctcttttc gaattttgaa agggttgttg atcagcagcg tttgtacaac
    16021 ttggaaggca ttggaaacta cccagtataa tgaaagaccg ctggctaaat tgacacccat
    16081 cactaaaatc attacaggca tcacgaaatt catgatcttc attgatgcat tcgattcaat
    16141 ttgactcatg ctagataaat acgtacttgc aaaagtaaag attgctgcta aaatcggcaa
    16201 aataaatgtt ggatccgcgt tccctaagtt caaccataag aaatgccctt gtttcaactc
    16261 tggtacacgt gaaatagatt gccataacgc catcaagatt ggcatttgta ctaataaagg
    16321 aagacaacca gcatatggat tgacgccatt ttctgcatat aaacgctgct gctcttctct
    16381 taacttactt tgtgtttccg gatctcttga actatattgt tgctgcagtg cttttaattt
    16441 cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg aaatgcatca acggcaatag
    16501 gatcacgcgg atgatcagtg taaataaaat gatccctatc cctgcattac caaatgacaa
    16561 cgccttgatc gcctcagcaa agtaataaac aatataacga tcccaaattc ctgtactctg
    16621 tgcgctgact tccccagttc cgcaaccgga taagacgatg accaaactaa cgataccggc
    16681 catcaaaagt aaacgtttgt atttcttcac ttactctttt cctctctgtc gattattttt
    16741 gcaagtttta agacgtggac taaattggag cgaacctcat cataagtcaa ccccttcaca
    16801 cttggtctag caatcacaat aaaatccaac tccggatcaa tttcttcttt tatagaataa
    16861 actgctgctc ggatttttcg tttgacttca ttgcggttta ccgcgttgcc gacctttttt
    16921 cctacagata aaccaactcg aaaatgaggc tgtcccggtt tttccaaacg gtaaacgacg
    16981 aatttgcggt tagcaaaaga atctcccgta tggaacacgg cttgaaactc tcgttctttt
    17041 tttactcgat acgcttttct cattagtttt ctttcctttt ctatctctca cttacgctca
    17101 taatcatacc ataattacga ttgtcctttc atcatttccc taaaaaagag tataaaaaaa
    17161 accactgaaa ccccagtggc ctaagctgaa agaacttttc ttcctttgcg acgacggcta
    17221 gctaacacac gacgtccatt tttagtactc atacgtttac ggaatccgtg aactttttga
    17281 cgtttacgtt tatttggttg gtatgttctt ttcatatatt ccacctccaa aatgttctta
    17341 tctattttat aaacacagac atactttgat agtataacga caaagtagtg attttgtcaa
    17401 tataaaatag aagatcgatt gttaaaactt atccacaaga ttattttttt ctatgggaat
    17461 cctatgtgga aaaagttatg cacagggatt atccacagat aataaaaggc tggttttaca
    17521 cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggc gatttcctgt gtattccgtc
    17581 tcctaactat tggtatatct gtatttttta tccacagacg caagaggaaa tctattggtt
    17641 tttacgagtt ttccacctgt gcattagtgg tgtggaaaac tttttttgac aactgtgttt
    17701 ttctcttttt tctttttgtc gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg
    17761 gtttctgtgg aaaactattg aaaaaaatgc taaattagta ctagttattc actttttaat
    17821 gaaggaggag gccttatatg gtatccctcg atgctttatg gaatgaatta aaagcaacat
    17881 accaaaagga tttatcgcct gctagctata atacatggat cgaaacagcg aaccctcgaa
    17941 cccttgatca aaaccagctc gttgttgagg ttcctagcaa gattcataaa gaatattggg
    18001 aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat gttatcagga aatgagatta
    18061 tccctcgctt tattactggc gaagaagcag agcaagaaga agttatagaa gaaaaaaatc
    18121 caaaagttgt ggcacctagt ccgctaaaaa aagccatgct gaaccctaaa tatacctttg
    18181 atacgtttgt cattggtaaa ggaaaccaga tggctcatgc agcagcgctc gttgtagcag
    18241 aagatccagg ttctatttat aacccgctct tcttttatgg aggcgtggga cttggtaaga
    18301 ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa
    18361 aatatgtaag cagtgaaact tttgctaatg atttcatcaa ttcgatccaa aacaaaacag
    18421 cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact gctagtggat gacattcaat
    18481 tttttgcaga aaaagaagct acgcaagaag agtttttcca tactttcaat gccttgtaca
    18541 atgaagggaa acaaatcgta ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc
    18601 aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt tgatattaca ccgccggatc
    18661 ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag
    18721 atgacacact aagctacatc gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg
    18781 cgcttgttcg agtgcaagct tttgctgcga tgaatagtga agatatttcg actagtctag
    18841 ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc
    18901 agatccaaga agaagtggca aagtattatc acatccaatt gaaagatctc aaaggaaaga
    18961 aacgcgtcaa atctattgtt gttcctagac aaatcgctat gtatttggca cgtgagctca
    19021 cagataattc tttaccgaaa atcggagctg aattcggtgg caaggatcat acaacggtta
    19081 tccatgctca tgaaaaaatc cagcagctga tggattctag tgtcagtatg caaaatgaag
    19141 tatcagaaat caaaaacttt ttactaaatt aatttgtcga ttctatcaac tgaaaatagc
    19201 atgtggataa aagaaaaaaa cgttcaaaag ttatacacac tttttccaca gctgtttttt
    19261 cgtttctctc ttacgttttt tgagtt
//
LOCUS       DAZUKD010000050        18677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000050 DAZUKD010000000
VERSION     DAZUKD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.051"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..427
                     /locus_tag="ACG8N9_001500"
     CDS             <1..427
                     /locus_tag="ACG8N9_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4897105.1"
                     /translation="KLADQKIVQIFEENIGISLTRYEIMMRLKEENPLLQSSLQEKLQ
                     IDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKHCEMDHKDTGSVLS
                     PAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            411..1040
                     /locus_tag="ACG8N9_001501"
     CDS             411..1040
                     /locus_tag="ACG8N9_001501"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4897106.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1394..2872
                     /gene="msr(C)"
                     /locus_tag="ACG8N9_001502"
     CDS             1394..2872
                     /gene="msr(C)"
                     /locus_tag="ACG8N9_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4897107.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2919..3365
                     /locus_tag="ACG8N9_001503"
     CDS             2919..3365
                     /locus_tag="ACG8N9_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4897108.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3564..4079)
                     /locus_tag="ACG8N9_001504"
     CDS             complement(3564..4079)
                     /locus_tag="ACG8N9_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4897109.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4196..5398
                     /locus_tag="ACG8N9_001505"
     CDS             4196..5398
                     /locus_tag="ACG8N9_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4897110.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            6013..6339
                     /locus_tag="ACG8N9_001506"
     CDS             6013..6339
                     /locus_tag="ACG8N9_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897111.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(6456..6800)
                     /locus_tag="ACG8N9_001507"
     CDS             complement(6456..6800)
                     /locus_tag="ACG8N9_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897112.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(6876..8123)
                     /locus_tag="ACG8N9_001508"
     CDS             complement(6876..8123)
                     /locus_tag="ACG8N9_001508"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4897113.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(8120..8926)
                     /gene="proB"
                     /locus_tag="ACG8N9_001509"
     CDS             complement(8120..8926)
                     /gene="proB"
                     /locus_tag="ACG8N9_001509"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4897114.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            9156..9683
                     /locus_tag="ACG8N9_001510"
     CDS             9156..9683
                     /locus_tag="ACG8N9_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4897115.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9746..11116
                     /gene="radA"
                     /locus_tag="ACG8N9_001511"
     CDS             9746..11116
                     /gene="radA"
                     /locus_tag="ACG8N9_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4897116.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            11150..12307
                     /locus_tag="ACG8N9_001512"
     CDS             11150..12307
                     /locus_tag="ACG8N9_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4897117.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            12477..13937
                     /gene="gltX"
                     /locus_tag="ACG8N9_001513"
     CDS             12477..13937
                     /gene="gltX"
                     /locus_tag="ACG8N9_001513"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4897118.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    13934..14173
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14259..14795
                     /gene="epsC"
                     /locus_tag="ACG8N9_001514"
     CDS             14259..14795
                     /gene="epsC"
                     /locus_tag="ACG8N9_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4897119.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            14792..16204
                     /gene="cysS"
                     /locus_tag="ACG8N9_001515"
     CDS             14792..16204
                     /gene="cysS"
                     /locus_tag="ACG8N9_001515"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4897120.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            16201..16596
                     /locus_tag="ACG8N9_001516"
     CDS             16201..16596
                     /locus_tag="ACG8N9_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4897121.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            16589..17449
                     /gene="rlmB"
                     /locus_tag="ACG8N9_001517"
     CDS             16589..17449
                     /gene="rlmB"
                     /locus_tag="ACG8N9_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4897122.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            17453..17983
                     /locus_tag="ACG8N9_001518"
     CDS             17453..17983
                     /locus_tag="ACG8N9_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4897123.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            18060..18638
                     /locus_tag="ACG8N9_001519"
     CDS             18060..18638
                     /locus_tag="ACG8N9_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4897124.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT         6045 a         3366 c         4053 g         5213 t
ORIGIN      
        1 caaattagcc gatcaaaaaa tcgtccagat ctttgaagaa aatatcggta taagtcttac
       61 tagatacgag atcatgatga gactgaaaga agaaaatcca ttgctacaaa gcagtttgca
      121 agaaaagttg cagattgatc aaggagcagt tactcgtcat ctgaaggtat tggaagcaaa
      181 tggctatgtt tcgcgtcaga gaaatcctca gaacaaccgg gaagtgtatg tagcgctaac
      241 agaaaaagcc cagaagaaaa tcaagcattg tgaaatggac cataaagata caggttccgt
      301 gctatccccg gctttcagcg atgtagaaat gaaacaattt ctaacacttt tagataaatt
      361 gaatacccaa ctttgcaata taacaacaga aacgaaagag gttaataata atggaacaaa
      421 aaaatgattt ttcaacaatt atgactggta gaagatccgt tcgcgtatat gatgaaacat
      481 acaaaatccc acacgaagaa atgctggaaa tgatcaaaga agcgacgact gcaccttctt
      541 ctgtcaatat gaaaccatgg cgctttgtgg tcgctgaaag cgaggaagca aaagaaatct
      601 tgcgtccttt gatccgtttc aatacgagac aaaatgatac ttcttctgca atggtcatga
      661 tttttggcga tatgcaatgt tacgaatatg gcgaagaaat ctacaaccaa gcttacgaat
      721 cagggaagat gccaaaagaa gtcagagatc aacaattagc tgcgatcatt ccttattaca
      781 agagtttttc aagagaagaa atgaacgatg tagtcaaagt cgattctagc ttagcggcta
      841 tgcaattcat gttagttgcg cgtgatcatg gctatgaaac aaatccaatt ggtggattcg
      901 aagcagatca gttggcagag gcatttggtt tggataaaga ccgttatgtt ccagttatta
      961 ttttatcaat aggaaaagcg gtagaagaag gttacgagtc tgttcgttta gcaccagaaa
     1021 agatcactat gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg
     1081 aatcgtgtgt ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt
     1141 acgtttcgaa cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt
     1201 tcatggatgg agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag
     1261 atttggtgat ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt
     1321 ttgcaaacag atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag
     1381 gagtgtaaaa catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg
     1441 caaccagcta gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat
     1501 cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt
     1561 tcccgatcat gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta
     1621 tctcgctgaa gcaaaggacc taaatttgga attagccagt catttccagt taagactgga
     1681 agaaacttca gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct
     1741 tctttcttct tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa
     1801 aagtattgat cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag
     1861 tcatgatcgc tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga
     1921 aatccgagag ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa
     1981 gactcagcta cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat
     2041 ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca
     2101 acaaatcaga ctggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat
     2161 ccaaaagaat gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc
     2221 gcagcagatg aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta
     2281 tcccatcatg ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg
     2341 tgattttcag ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa
     2401 gacaacctta ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt
     2461 aagctttcaa gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg
     2521 ttttgtcatg agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt
     2581 agggtttgct caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac
     2641 ccgtctgacg attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc
     2701 gactaatttt attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc
     2761 gggaacgatt ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt
     2821 ttatgaaata aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta
     2881 cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag
     2941 gaataatttt tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt
     3001 tatggctgca tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg
     3061 tggtggcttt cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta
     3121 aactaccagt aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg
     3181 ttttaggaat ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg
     3241 atcaattatt ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt
     3301 tgcagttgat catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg
     3361 aatgatggaa cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg
     3421 aatgcagttc tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc
     3481 tttttctttt ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt
     3541 tctagaaagg ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc
     3601 tgacaccatt ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt
     3661 ccaaattctt gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg
     3721 attttgttca gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc
     3781 gtcgaaaaag catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc
     3841 tctttgatcc attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt
     3901 tgagagtgct cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc
     3961 gttgtataga tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca
     4021 tacccagaac gtggtttgga taaaagcacg cataataata tataggctaa agtattcata
     4081 aaaatcctac ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac
     4141 atgtgcatat tgacatgtaa aataaatcat gtatgatgct aatagagaga aagggatgga
     4201 aatattggag aatcaaatag atatacaaga acaaacaaat gaagaaacaa aggggaaaag
     4261 ttcagcgcta gttgtagcag ctacctgtat gatcgctttt atgggagtgg gtctagtaga
     4321 cccaatctta aaaacgattg ctgcacagtt aaacgctacg ccagctgaaa cgaccctgtt
     4381 atttactagt tacatgttag tcactggtgt ggtgatgcta tttacaggtt tcttatctag
     4441 tcggatcggt ttgaaaaaaa cattgatgac aggattgttt atcatcgttg tattcgctgg
     4501 tcttggcgga ttatctggga atatcggaat gctgattggg ttacgtgccg gctggggtgt
     4561 cgggaatgct ttatttgtct ctactgcttt agcaacgatc gtcagtattt tagttggtaa
     4621 cacagaaaaa gcaatcatga tgtatgaagc agcacttggc tgcggaatgg ctgttggccc
     4681 tctagtcggt ggggtcttgg gcagtggttc atggcgttat ccattttttg gggtagcatt
     4741 tttgatgttt tgcggtttta tagccctagc ggtactttta cctgaaactg agaaaccaaa
     4801 gagaaaagta ggcctttggg aaggtgtcaa agcgttaagc aatcataaac ttcgttcagt
     4861 tggactaatc gcattgttat ataatttcgg attctttact ttacttgcgt atgcgccatt
     4921 tttattgatt ggctattctg aattagaagt cggttttgta ttttttggct ggggtgtgct
     4981 tctggctatt gcttcgattt tcgttgcacc ggctcttgag cggaagttta cgacttatcg
     5041 gacaatttta gcagcatttg tcttattcat tatttgtttg ctgttattag gagctggaac
     5101 ttctatgcca gtccttgtac cgatcagcat tgtgttagca ggatttttcc aaggaattat
     5161 caatacactg ttgacgacga ttgctatgga aataccaggt ctgcaacgaa acgttgcttc
     5221 gtccagctat agttttgtcc gctttttcgg tggagcttta gcaccattta ttgcaggtgt
     5281 gattggtgaa catattggtg aaagctatgc gttttatttt gcaggtgcca ttgttcttat
     5341 cagtgtatgt tgtatcattt atcaccgtaa ttattttgct acaaatgaag aagaataaga
     5401 aatcagaggt tgtgaaaaag ctgtcctgca tcaagtcata agaacagcca aacaaaaata
     5461 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
     5521 tttatcagga ggttgtaaaa tcagcgtttt gtcctgagta ttagagaaac aaacggaaaa
     5581 atagttcctc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
     5641 gtttaacaga ggttgtaaaa tcagcgtttt atcctaagta ttagagaaac aaaacggaaa
     5701 aatagcttct catattttac cgttttttga gctaataccg agggtcagct gattgaacgc
     5761 cgtttaccaa gaggttgtga gactgtcgtt taactctgaa tcacaaggaa caatttcaaa
     5821 aaacagtttc tcatgtttgt tgaatattgt gacttatgat tgaggagttg gcagaagaac
     5881 accatttatt cggaaaaagg ggatgtgaca cgatttttgt cacattccct ttttttctca
     5941 aaataatgaa actggaaaat tttcagtagg taagctaaaa ttcatttaag aaaaagaatg
     6001 gggggataat agatgctgga agaatatttt caggaagtcg ggttgacact cgttgaatta
     6061 cccattgaat tttcagatga tcggttgatt tatcgtggct atatcaaaga attgccaata
     6121 atcaatgcgg aaggtgaaac tagaaaagcg atgcagcaaa aaatagctgg tatgtatcag
     6181 gtgtatcggg aacagctact ggctgaatca gcggaagaag aaaaagaaaa aacgatgaac
     6241 ttgtccatgg atgagctgct tcgttattat gatggcgaaa catttgacgg attttcgctt
     6301 cttaacgaag aggaaacaga tttaccaggc agctggtagg cgaacgccgt ttatatggaa
     6361 aaagaggctg tgacaaaatc tttgtcacag cctctaaaaa gtccggcagt tttttgccga
     6421 tcttcaaggc actctactta tcgtacggat attttttagc ggaaagtccg attcaagtgg
     6481 tttttattca gctgatattt ataagataag tgaaaaatcc cagcaatcag tagcgtaatc
     6541 gtataagcga ttgaatgatc gaaaaaagaa tagtaattga ttgtcgctaa taagcataat
     6601 gcaaccatgt aactaatgtg caagcgtgta accagccctt tttgcccgat tttgtcaaaa
     6661 tactgcgacc atttttcccg ttgactccct atgcttcgtt ttttgaattg aaagaaaagg
     6721 gataaaatac cgactcccag aaaagcagcg atattcagag aaataaggta aataaaaaaa
     6781 atcattaaaa tttcaaacat attgacacct ttctagttgg atagaacctt tacagaaaaa
     6841 aggcaaaagg cgggaataat gtttcacacg aaacattatt cgcggatttg tccatcgcca
     6901 taaatgatat attttgtgga agttaattca tttaacccca ttggaccacg ggcatgcaac
     6961 ttttgagtag agataccaat ctctgcacca aaaccaaata ccgatccgtc tgtaaaacga
     7021 gtggaggcat tgacatagac agccgccgcg tccacttcat ttaagaaacg ttgactagcc
     7081 gcataactat ctgtcacgat cgtttctgaa tgtttagtat tatgccgatt gatatgtgca
     7141 attgcttcat ccaaagaatc gaccactttg acagcaagga tataatctag atattcagta
     7201 tcccaatctg tgtcttctgc taaagcagcc gtctctagat attctaacgc acgagcatcc
     7261 gcacgtaaag agaccccgtg ttctaccagt tctttttcaa tagctggcaa gaaaaaaggt
     7321 gcgatctcag aatggatcag caatgtttct gccgcgttac agacagatgg acgggaagct
     7381 tttgcattga cgatgatgtt cactgccata gataactgtg cttctttatc gatatatacg
     7441 tgattatttc ctgtacctgt ttcaatgata ggtaccgttg cctgttcttt gactcgttgg
     7501 attaattttg ctccgcctcg aggaatcagc acatctaagt attctgtcaa tcgcatcaac
     7561 tgtcctgcag tttcatgaga agtatcatcg acaaactgga tggcataagg agattcgcca
     7621 gcactgacca gtacttgctg caataattgg actaataatt gattcgagaa gaatgcttct
     7681 ttgccaccgc gaaggataac ggcattccct gatttgaatg ctagactagc tgcatctgtc
     7741 gtgacatttg gacgagattc gtaaatgatg ccgatcacgc ctagtggcac gcgctttttg
     7801 ccgattctca atccgtcggt gttcgtccac atctcatcta cttttccaat aggatcgtct
     7861 aacgaagcaa ttttttcgat ctctgtagcc atggcattga ttcgctcagg tgtcagctgc
     7921 aagcggtctt gcatcgtttc actgatattg ttttcgactg ctttttctaa gtctttatga
     7981 tttgcttcaa tgatcgcttg ttgattcttt ttgatggttt gtgccatttg aacaagtagc
     8041 tcatttttgc ggtttgtagg cataagtgct agttgttgcg cgctatcttt tgcctgttgt
     8101 cctaattgca tcaagtcagt catttcaagt cctccttaaa taaagtacct acttcttttc
     8161 ctgcaagaat atcaaaaata attgccggat tttttccatt tgctaaaatc attgcgctgt
     8221 ttgcttcaaa tatcctagaa gcggctttta atttactctg cataccgcct gttccaaatg
     8281 tgctaccagc accacctgct tgtgccagaa tcttttcatc gattttatcg atatagcgat
     8341 acatctgagc ctcgggattt gtagtcggat tatctgaata aaacccgtcg atatcagaaa
     8401 ggacgatcaa aaggtctgcc gcgacaagtt ttgccatgat ggctgacagc tgatcattat
     8461 cgccaaattt tgtcagatga tctaattcat ccactgctac agtatcgttt tcgttcacga
     8521 tagggatcgt gtccataatc aaaagctgtt ccaatgtatt cctcacattt ttacgacttt
     8581 caggataatc taccacgtct cttgtcacga ggatctgtgc cgtctgttga ttatatacac
     8641 tgaatctttg attatagata ttcatcagtt cggcttgacc aacagccgcc gcagcttgct
     8701 gttcaggaat caaagtcgga cgttctgaca aatgtaattt atgcatacca acgcctacag
     8761 ctcctgatga gactaagatc acttcctttc cccgatttct aagatcagaa aggacaaaag
     8821 ctaactgatc gattgccgca agattgatat ttccattggg ataaatcaat gtacttgttc
     8881 ctactttgac aataattcgt tttgcatttt taatttcttt acgcatgtga cacctcaatc
     8941 aaatatccac tcattaattt cgttctattc tacttgtttt gacgagtaaa gtcatgtaaa
     9001 aaatagtttt tttgtacaac ttgcagtcgt ttggaaaaag aacgaattaa tggtttactt
     9061 taagatagca gaatttcttg aagaatttgt aaagaaagtc ttttacagcc tcggatatgt
     9121 tagaatagga gtagtttatt tagggaggaa aaccggtgag aaaaagagga tttgaagtta
     9181 tttctaaata taaagatcaa aatatcaatc taccgcagcg cgctacacat catgctgctg
     9241 gctatgattt tgaagcagca gaagaggtcg tgattccaag tatctggaaa gcaggctgga
     9301 aaaaggggtt ggaaacagtc gcagatggat tgaaaaaaga tgtatcagaa aatgaaaaac
     9361 aattgaaacc aaccctcgtg cctactggaa tcaaagctta catgggagaa gatgaatttt
     9421 tgcagcttgc taaccgttca agcaatccgt tgaagcggtt tttagtgttg acaaacggag
     9481 taggtgtgat tgacagtgat tattacaata atgaggcaaa tgaaggccat atcatgttcc
     9541 aattcaccaa ctttggtttc aaggatgtag tgatcaaaaa aggcgagcgt atcggccaag
     9601 ggatcttttt gccgttttta aaagcagatc aagatcaaac gactgctcaa cgtcaaggcg
     9661 gatttggttc ttccagtaaa taaaacgtta aagatactta agcttgattt aatgtctcat
     9721 gattaggata aaaggaggcg gtacaatggc aaaaaaatca aaagtacaat ttgtttgcca
     9781 aaattgcggg tatgtgtcgc ctaagttctt gggacgctgc ccaaattgcg gaaaatggaa
     9841 tacgatggtc gaagaaatcg aacaagatac aacggaccgc agaactcgga caagtttgac
     9901 aggagaaaaa gcaaaaccaa caaaaatcgc agacgtcgtc cctaaaaaag aacctcggat
     9961 caaaacaaaa ttagaagaac tgaaccgtgt gttaggcgga ggtgtggtgc cagggtctat
    10021 ggtcttgatt ggtggcgatc caggtatcgg gaaatccact ttgctgttac aagtttctca
    10081 gcaattagca gccataggcg gaaaagtctt atatgtatcc ggcgaagaaa gcgcagagca
    10141 gataaaatta cgcgcggaac gcctcggaag tatcaacaca gaattttatc tatacgctga
    10201 aacagatatg aacgaaatca gtcgtgccat cgaacatatc tcccctgatt atgttatcat
    10261 tgattccatc caaacgatga cgcagccaga catcacaagt gtggcaggaa gtgtcagcca
    10321 agtaagagaa accactgcag aactactcaa aaccgctaaa acaaatggga tagccatttt
    10381 tatcgtcggt catgttacca aagaaggctc gatcgccggc ccaagaatgt tagaacatat
    10441 ggtggatacg gtgttatatt ttgaaggtga acaacatcat agttttcgga ttttgcgagc
    10501 agtaaaaaac cgatttggct caacgaatga gattgggatt tttgaaatgc atgagcatgg
    10561 attggaagaa gtcgctaatc cttcacagat tttcttagaa gaacgcctag acggtgcaac
    10621 tggctctgct atcgttgtgg caatggaagg gacacgtcca attttggtag agatccaagc
    10681 attagtcaca cctactatgt tcggaaatgc caaacgaaca acaacaggtc ttgatttcaa
    10741 tcgggtatct ctgatcatgg cagtgctgga aaaaagagct gggttgcttc ttcagaatca
    10801 agatgcttac ttaaaagctg caggtggggt aaaattaaat gaaccagcga ttgatttagc
    10861 tatcgctgtc agtattgctt caagctataa agaaaaagga acacagccaa cggaatgttt
    10921 catcggtgaa attggactga ctggagaaat cagacgtgtc agtcatattg aacaacgagt
    10981 caaagaagtc caaaaactcg gcttcacaaa agtataccta cctaaaaaca acttaggtaa
    11041 ctgggaagcg ccaaaaggaa tcgagatagt tggagtagca actttggctg aaacgttgaa
    11101 gcgtgtgttc cgctaactaa taaatgattg tcgaaacgga ggaaaaacga tgcaaaaacg
    11161 tgtcattact ttgctgatga tcattgtcgg cgcaagcttg ggtatttcat ttttaccctc
    11221 agcttggcag gcaatcggtc aacagtcaaa cggatggctg aataatacat ttaccaacag
    11281 cctgttaggc gcacttattt tctttttatt atctttgctg ttggccaaac atatctcagc
    11341 tattatcaaa caagtagaac aaaaactaag tgaggtcagc ttgacttatc ttctcttcgg
    11401 tgcgatcggt gcgatcatcg gattgacttt aggtgttgtg atctcgaccc ctctttacaa
    11461 tatggaaatt ccattcgtca atagtgtatt gccgatttta ctgatgatca tcttaggcta
    11521 tctgggattg agaatgggaa cgacacgaat cgaagaatgg aagaaagttt ttggttcacg
    11581 aagcaaaaaa atcgagcaag aaacagctag tcaagttgag agtcaactgt tagaacgaaa
    11641 atcaggagaa aatttccata aatataaaat cttggataca agcgtgatca ttgacggtcg
    11701 gatttatgat attacaaaga ctggattctt agaaggaacc ttgatgatcc ctaattttgt
    11761 tctttacgaa ctacaataca tcgcggactc aggggatagt ttgaaacgtg tgcgtggtcg
    11821 tcgcggatta gatatcctaa atgccttgca aaaagaagag ggtatatctg tcgaaatgta
    11881 cgatggagac tttgaagata tcagtgaagt agacagcaaa ttgatcaaat tggctaagct
    11941 tttagatggt gtcattgtaa ccaatgacta taacttgaat aaagtctctg aattccaaaa
    12001 tgtacctgtt cttaatatca atgcgttggc aaatgccgta aaaccagtcg tcatccctgg
    12061 tgaaacgatg aatgttctag tagtaaaagc cgggacagaa cgccaacaag gagtagccta
    12121 ccttgatgac ggcacgatgg ttgtcgtaga agatggacag cattacatga atgaacgtat
    12181 cgaagtcgtt gtaacaagtg cccttcaaac agctgctgga cgcatgatct ttgctaaacc
    12241 tgcacatgca ggacgaggaa tcgacaataa aaaagagaca gagaaaaaga ctaaacaatc
    12301 aaaataaaaa agaaattcag ccaactggta aggaaattca accagttggc tttactattt
    12361 accttagaaa aggtaaaatt tgtgctgagt ggctttaaat aaatgatatt tgagaagccg
    12421 taactgacgg aatttttgct gttagtcaaa gtctagtttg aaagaagagg aagaaaatga
    12481 cgaaagtacg cgtacgctat gcaccaagtc caactgggca cctgcatatt ggaaatgcaa
    12541 gaactgcatt attcaattat ttgtttgccc gccacaatga tggagacttt attattcgta
    12601 ttgaggatac tgaccaaaaa cgtaatatcg aagatggcga aaaaagccag ttggaaaacc
    12661 ttgcttggtt gggaatgaat tgggatgaat ctcctgaaaa tcctggagaa tacggccctt
    12721 accgtcaatc tgaacgtaaa gaaatctatc aaccgctgat tgatcagctg cttgctagca
    12781 atcgagcata caaatgctat tgcacagaag aagaattaga agcagaacgg gaagcacaac
    12841 gcgcaagagg agaaatgcct cattacgcag gaacatgtgc gaacttgaca cctgaagaac
    12901 aagcagaaaa agaagcacaa ggtctagaat ctgttgtacg tttccgtgtg cctcgcaata
    12961 cagaatacgc atttacagat atggtgaaag gcgctatttc gttcgaatcc gataatatcg
    13021 gtggcgactt tgtcatccaa aaacgcgatg gtatgccaac ttataatttt gctgttgcgg
    13081 tcgatgatca tttgatgaaa atcactcacg ttttacgtgg agatgaccat attgccaata
    13141 caccaaaaca actgatgatc tatgaagcat ttggctggac accaccaacc ttcggacata
    13201 tgacattgat catcaacagt gaaacaggta aaaaactaag caaacgtgac gaatcaatct
    13261 tgcaatttat cgaacaatat cgcgaattag gctatctacc agatgcaatg ttcaacttta
    13321 ttgcattatt gggctggtca cctgtgggcg aagacgaaat cttcgaacac caagaattga
    13381 tcaaaatgtt cgatccaaaa cgcctaagta aatcacccgc agctttcgat gcgaaaaaac
    13441 tgcaatgggt aaacaaccat tacatcaaag caatggatct agatgcattt actgacatgt
    13501 gtctgcctta tctaatcgct gatggacgtg tagcagaaaa cccagatgaa aagacaatcg
    13561 aatgggtgaa gaagatcgtt agcctgtatc aacctcaaat gagctatgct gcagaaatcg
    13621 ttgatctgtc tacgctattt ttcaatgaac atcctgtatt gaatgaagca gcaaaagaag
    13681 tacttgctgg tgaaacagtt cctactgttc tccacgcatt caaagcacag ctagaacaaa
    13741 tggaagtagt agatgctcca actgtcaaag ctgccatcaa agctgttcaa aaagaaactg
    13801 gcgtaaaagg aaaaaatctt ttcatgccaa tccgtgtagc tgtatcagga caaatgcatg
    13861 gtccagaatt gcctgaaacg atcgaattgc ttggtcgtga aaaatcatta gcacatttaa
    13921 atcaagtact gaactaaaca cgatgaacag aaaaaagtaa gcagctgatc attacaagag
    13981 agataccggg tggtgcaagg tatcaatgaa tggttgccga aatgcctctg ggagtggtct
    14041 tggcgaaaca cagaaaacag gaaaaactgt tttataggaa acaaagtagc caagaacgta
    14101 taacaggcgt tagctgtcag agataatcat tgattatgaa gaaaagtgga accacgcgga
    14161 aagcgtcttt tgataaggaa acttatttaa aaggcgcttc ttttctttat aagaagatga
    14221 tttcctacat aaaattctta cgaaaggagt ggaaagtcat gagttggttt acccgagcga
    14281 ttgatgcagc aaagagaaac gatccggctg cacgttcgac agctgagatc gttttgacat
    14341 accctggttt tcatgccttg ttttggcatc gtttctctca ttttttatat cagcaccggc
    14401 tctttttatt ggcgaagatc aatgctcaat tctggcgttt cctaactggt gttgagatcc
    14461 atccaggagc aacgattgga actggcgtgt tcatcgacca tggaatggga atcgtcattg
    14521 gcgaaaccgc agaaatcgag gacgatgtga ttttattcca tggcgtaaca ttaggcggca
    14581 caggaaaaga aacgggtaaa agacacccaa ctgttaaaca aggagccatg ctctctgcga
    14641 atgcgcagat attaggaccg gtaacgatcg gaaaaaacgc caaaatcggt gcaggtgccg
    14701 ttgtgttgaa agatgttcca gacgacgcta cagcggtagg agtaccagca aaagtcgttc
    14761 gaatcaaagg agaaaaagtg aggagatcag aatgataaaa atctacaata cattgaccag
    14821 aaaaaaagaa gaattcatcc ctatacaaaa gggacatgtc ggtatgtatg tctgcggacc
    14881 gactgtgtat aattatatcc atatcggaaa tgctcgtagc accattgctt tcgatacaat
    14941 tcgacgctat tttgaatatc gaggctataa agtcgatttc gtatcgaatt tcacggatgt
    15001 agacgataaa atcatccgta cagcaaacga attaggtatt acagcaccag aagttgctga
    15061 tcgtttcatc cgagcatttg aagaagatac gaaagcgttg aatgtcgaac ctgctacact
    15121 gcatccaaga gtcatggatc atatgccaga tattttagca tttattcaag tgttggttga
    15181 aaaaggctat gcttatgaat cttcaggtga tgtgtattac cgtacccgta aatttgaaaa
    15241 ttatgggaaa ttaagtgacc aatccatcga tgaactggaa gtcggagcaa gtcagcgtac
    15301 aggggaagaa cagcagcaaa aagaagaccc attagatttc gctttatgga aacaagcaaa
    15361 acctggcgag atttcttggg attcaccatg gggcaaagga cgtcccggtt ggcatattga
    15421 atgttccgtt atggcaacaa aacatttagg agaaacgatc gatatccatg gtggcgggca
    15481 agatttagaa tttcctcatc atgaaaatga aatcgcacaa agtgaagcga aaacagggaa
    15541 aaaattcgct aattactgga tgcacaatgg ctatgtaacg attggcgaag acgatgaaaa
    15601 aatgagtaaa tctctaggga atttcatcac tgtacatgaa atgatcaaaa acgttgatcc
    15661 acaagtcttg cgtttcttca tggcgaccac acaataccgt cgtccgatcc gctacagtga
    15721 aacgacgatg aaagaagcag ccaccaattt acaacgactg aagaatacgt ttgacaattt
    15781 gaaattccgc caagaaactg cctcagagaa tttggcagat gatgcggata aaatcgcttt
    15841 gttagaaaat ttagaacagc gattcatcga agaaatggac gatgatttta atgcagcaaa
    15901 tgggatcaca gtggtctatg agcttgctaa gtggctgaac acttatctag accagccgaa
    15961 agtttctgcc gctgtcttgg cacaaggaga agctttattc tcgcaatggc tttcgatctt
    16021 cggtatttta tttacagcag aagagttatt agatgaagag attgaacaat tgatcgaaga
    16081 acgaaatcaa gcaagaagca atcgtgattt tgtccgaagc gacgaaattc gtgatttgtt
    16141 gaaagaaaaa gggatcttat tagaagatac accgcaagga acccgatgga gaagagaagc
    16201 atgagagatt acacacaatt gaacggttta gccttggctt atgttggaga tgcgatttac
    16261 gaaatctatg ttcgtgacca cctgattgat caaggagaaa caaaacccaa taagctgcat
    16321 cgtgcggcaa cgaattatgt ttctgcgaat gcacaagcct ttttgattca gcaaatgctg
    16381 gaagaacatc ttttgacaga agaagaagaa ctttattatc gcagaggaag aaatgctaaa
    16441 agccatacga gcgcaaaaaa tgctgatgtt acgacgtatc gggtcgcaac agggttcgaa
    16501 gcgctcatgg gctatctgca tttgacaaaa caaaccgagc ggatggaaga attgatccgc
    16561 tggtgtattc agaaagttgg tgaaaaaaat gggtaaagaa agaacaaaca aacatcaatc
    16621 gaaacgtgga tttgaagaca aaaaacaaaa tagatattca aataaaaagc aaactggacg
    16681 aaatcagtca gtttctgatc aaaatgaaga agcattagaa gataatttcg tctttgggca
    16741 ccatgccgca gtagaagcgc tacaacaagg gcgagggaat aaactctttc ttcaagaaga
    16801 ttcacgagga gaaaaagtag aagaactgaa agaaatcgca cgtgaacaag ctgtgccagt
    16861 caaatgggtt cctaaacaaa aattagaaac tttgagcgat cacggcgtgc atcaaggagt
    16921 cgttcttgcg attacacctt atgaatattt atcccttgac caactgattg aacgaacaaa
    16981 agagaaaaaa gaagacccct tctttttgat tttggatagc atcgaagatc cacacaattt
    17041 tgggtcgatt ttacggacag cagacgcaag tggcgtcgat ggtgtgatca taccaaaaca
    17101 ccgagcagtt ggaatcacac ctatcgtaac aaagacttcc actggggcag tcgaacatgt
    17161 ccctgttgca cgcgtgacga atttggtaca agctgtgcaa cagctcaaag aaacaggatt
    17221 ttgggtattt ggcacagata tgaccggcac aaacttccaa aaatggaatg ccaaaggatc
    17281 aattgcttta gtcattggta atgaaggcaa aggaatgagt ccaggattga aaaaagaaat
    17341 ggatgaattg ctgacgatcc cgatgaccgg gcacgtccag agcttaaatg ccagtgttgc
    17401 tgctggtttg cttatgtatc aggctttttc ccaacgtcag ggggagtaga ccatgaaaaa
    17461 acagctgttg attgtagacg gctacaacat gattggttcg tggccggaac ttgtccagct
    17521 taaaaagcag gacaaaatgg cggatgcaag agaagctctt ttacatcgtt tatcaaatta
    17581 tgcgaaatat gaaggaatgg aagtcatcgt tgtcttcgat gcccaactag ttccgggcat
    17641 ccaacaaaat tataagaaat accagttgga tgttgttttt acgaaagaag acgaaacggc
    17701 tgacagttat atcgagagaa tcgctggtca gctgaatgac cgtttgacac aagtgacagt
    17761 ggcgacaagc gatttagcag aacagtggat gatcttttcc caaggagcat tacgaacttc
    17821 tgcttatgaa ttatataaga cgataaaaaa aacggaaaaa acaatagccc aagaaaataa
    17881 agatatgcat tttattgatt tccgacgtaa ttctccatgg aatgaagagc agttaaaaaa
    17941 attgtcccaa aaattggacg atttaatgag aaaaaataaa taacatatcg ttctaatcag
    18001 ttcgatgaat cattgatgat aagattcctc cataaacgaa tttatggagg aattatttta
    18061 tgtctgagga gttattgcgc gctgcacgta atggtgacga agaagcattt gctgccctgt
    18121 atcaaaaata ttttccactt ttactacgtt tgaaaaacac ctattattta agaaattatg
    18181 tttctgagga ttgggaacaa gaagcaagga tcgctttata tcaggcattg agaacttacg
    18241 aaaaaagcaa aggtgttagc tttggaagtt attataaaag cgttataagc aatcaaatct
    18301 attctctgtt gaggaagcag aacgcgctga agcgtcgtga tcaaaaagat gaagtctcaa
    18361 tcgaccaaaa aatagagaca gaaggaccag actttttagc agagatcaca ttgcaagaac
    18421 ctttagcaat tcagcatctt ttattaaaag aagcaatcga aaattgcgaa atccagtttt
    18481 caaaaataga agctaaaatt tttcattatt atatgcaagg cgttgatttt gtagacatgg
    18541 ctgaatgttt agcaatggat cacaagcaag taatgagcgc ttacgggaga gtaaaactca
    18601 aaataaaaaa acacttgaaa ttttataatg aggaataatt tagaaaaatc ttaatgagct
    18661 tcttatttat ttttatt
//
LOCUS       DAZUKD010000051        18494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000051 DAZUKD010000000
VERSION     DAZUKD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.052"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8N9_001520"
     CDS             99..746
                     /locus_tag="ACG8N9_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4897125.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8N9_001521"
     CDS             1139..3784
                     /locus_tag="ACG8N9_001521"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4897126.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8N9_001522"
     CDS             4094..5407
                     /locus_tag="ACG8N9_001522"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4897127.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8N9_001523"
     CDS             5397..6050
                     /locus_tag="ACG8N9_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4897128.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8N9_001524"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8N9_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4897129.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8N9_001525"
     CDS             6947..7147
                     /locus_tag="ACG8N9_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4897130.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8N9_001526"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8N9_001526"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4897131.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8N9_001527"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8N9_001527"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4897132.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8N9_001528"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8N9_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4897133.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8N9_001529"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8N9_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4897134.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8N9_001530"
     CDS             13269..13466
                     /locus_tag="ACG8N9_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897135.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8N9_001531"
     CDS             13619..14104
                     /locus_tag="ACG8N9_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4897136.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8N9_001532"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8N9_001532"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4897137.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15502
                     /locus_tag="ACG8N9_001533"
     CDS             14981..15502
                     /locus_tag="ACG8N9_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897138.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            15599..16336
                     /locus_tag="ACG8N9_001534"
     CDS             15599..16336
                     /locus_tag="ACG8N9_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4897139.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8N9_001535"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8N9_001535"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4897140.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8N9_001536"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8N9_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4897141.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5981 a         3200 c         4022 g         5291 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt gaagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattgct gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa tttt
//
LOCUS       DAZUKD010000052        18472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000052 DAZUKD010000000
VERSION     DAZUKD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.053"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..847)
                     /locus_tag="ACG8N9_001537"
     CDS             complement(23..847)
                     /locus_tag="ACG8N9_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4897142.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(820..1500)
                     /locus_tag="ACG8N9_001538"
     CDS             complement(820..1500)
                     /locus_tag="ACG8N9_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4897143.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1523..2461)
                     /locus_tag="ACG8N9_001539"
     CDS             complement(1523..2461)
                     /locus_tag="ACG8N9_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4897144.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2706..3557)
                     /gene="ispE"
                     /locus_tag="ACG8N9_001540"
     CDS             complement(2706..3557)
                     /gene="ispE"
                     /locus_tag="ACG8N9_001540"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4897145.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3711..6284)
                     /gene="mprF"
                     /locus_tag="ACG8N9_001541"
     CDS             complement(3711..6284)
                     /gene="mprF"
                     /locus_tag="ACG8N9_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4897146.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6425..7231
                     /gene="proC"
                     /locus_tag="ACG8N9_001542"
     CDS             6425..7231
                     /gene="proC"
                     /locus_tag="ACG8N9_001542"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4897147.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7310..8866)
                     /gene="rny"
                     /locus_tag="ACG8N9_001543"
     CDS             complement(7310..8866)
                     /gene="rny"
                     /locus_tag="ACG8N9_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4897148.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9277..10326)
                     /gene="recA"
                     /locus_tag="ACG8N9_001544"
     CDS             complement(9277..10326)
                     /gene="recA"
                     /locus_tag="ACG8N9_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4897149.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10435..11676)
                     /locus_tag="ACG8N9_001545"
     CDS             complement(10435..11676)
                     /locus_tag="ACG8N9_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4897150.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11916..12494)
                     /gene="pgsA"
                     /locus_tag="ACG8N9_001546"
     CDS             complement(11916..12494)
                     /gene="pgsA"
                     /locus_tag="ACG8N9_001546"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4897151.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12703..13626)
                     /locus_tag="ACG8N9_001547"
     CDS             complement(12703..13626)
                     /locus_tag="ACG8N9_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4897152.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14365..15651)
                     /locus_tag="ACG8N9_001548"
     CDS             complement(14365..15651)
                     /locus_tag="ACG8N9_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4897153.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15644..16912)
                     /locus_tag="ACG8N9_001549"
     CDS             complement(15644..16912)
                     /locus_tag="ACG8N9_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4897154.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17164..17625
                     /gene="mscL"
                     /locus_tag="ACG8N9_001550"
     CDS             17164..17625
                     /gene="mscL"
                     /locus_tag="ACG8N9_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4897155.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17684..18130)
                     /locus_tag="ACG8N9_001551"
     CDS             complement(17684..18130)
                     /locus_tag="ACG8N9_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4897156.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18223..18468)
                     /locus_tag="ACG8N9_001552"
     CDS             complement(18223..18468)
                     /locus_tag="ACG8N9_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4897157.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5269 a         4038 c         3231 g         5934 t
ORIGIN      
        1 taaggcttct tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt
       61 tgatgatatt tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag
      121 gtgtatctag ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa
      181 cactgatagc aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa
      241 gaaccatgat tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca
      301 caccagtaat cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg
      361 cggtatcttc atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac
      421 cgatggcaac gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg
      481 aaccaaacag ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata
      541 ctaacgctag cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc
      601 ggtagatcgt tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg
      661 taagtgtagg attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag
      721 aaagcgtatc agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg
      781 cgattccggc aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca
      841 acgccattga gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat
      901 atcttcatga tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg
      961 gagaagacga taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag
     1021 aacaaataag tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc
     1081 gccagacaat tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc
     1141 acgctgtaca tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata
     1201 tcttcctgaa cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac
     1261 ttgttgagga atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt
     1321 tatcattcct tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc
     1381 tgctccattt tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc
     1441 ttctagtaca ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac
     1501 cttacaacct ccattctaaa acttaattga tactttcttt taaagctttc agattttttt
     1561 ccatgacaga aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag
     1621 gattcaacac ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag
     1681 aggcattttc ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct
     1741 cagctaagcg acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt
     1801 ttaagtcgta ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt
     1861 ttgttgcatc ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat
     1921 aagcatcggc attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt
     1981 cagcaatcgt tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt
     2041 attcatgtga atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt
     2101 ctaaacggtc gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat
     2161 cctttaacga atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat
     2221 cggaaatttc tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag
     2281 ggatcaaaag tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg
     2341 gataaaaagt cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc
     2401 cacatgctgc aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca
     2461 tttatatgct cctttcaatg aacaaatcgt aattttacga tttattaacg tgaactaatg
     2521 tatcaaaata aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata
     2581 ttcttgatta aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat
     2641 aaaaaactga agaataccat tttaggattc ctcagttttt catcacctat tttacttctt
     2701 atattttatt gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta
     2761 tatactctct gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca
     2821 ctcatcaatg cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact
     2881 ggatgccgct ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga
     2941 taatcttgct ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc
     3001 gtatctactt ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca
     3061 acccagcact gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat
     3121 gccgtacttc cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc
     3181 agttcttcta acgaacagcc cagattccat aattggttga tccctcttag agcagctgca
     3241 cagtctgtac ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc
     3301 ttgacaccgg ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg
     3361 ttccgttcat ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt
     3421 atttcttcaa aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca
     3481 tgatacccat cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct
     3541 ttttcaatca attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca
     3601 tagggaaaaa taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa
     3661 cagctttcac agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct
     3721 gtgagcactt cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt
     3781 agctgcatgg tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc
     3841 caatgatcga catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta
     3901 ccataattat atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca
     3961 acattagcta atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca
     4021 ataaacagat tggctaacag atagtcagct aattcctcag attctttagg taagacccgc
     4081 aacaaatcat atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact
     4141 ttttgatctt tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt
     4201 cccacagaga agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca
     4261 gcttgatgta attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc
     4321 aaacgttgat attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca
     4381 atccctgctt cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag
     4441 ctttctgata tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac
     4501 tgattcgttg ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat
     4561 ttgtttcctt tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa
     4621 aaataactgt aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc
     4681 aataaacggt caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct
     4741 aaatattgat aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag
     4801 aaccacacat catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa
     4861 aagcgtccgc tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta
     4921 gcatcaaaga ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg
     4981 gataaccaca cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta
     5041 cgtgtgattg tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt
     5101 ttgactttta ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc
     5161 actgtctgat ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt
     5221 gcagacagat tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca
     5281 cttacgagca cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat
     5341 cgattgattt tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga
     5401 acataataaa acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac
     5461 aattgactca ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc
     5521 gtcagcattc ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata
     5581 tctattctca tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca
     5641 aaggatgcca gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt
     5701 ctctttagat aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag
     5761 atccaatagc ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga
     5821 aacgaccata cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa
     5881 accatttttc tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca
     5941 ccaccaaaac cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct
     6001 tttctttcca tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac
     6061 aacaacatcg gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg
     6121 ctttgttgtc ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg
     6181 acttgatttg ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta
     6241 tgttccttta tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa
     6301 cttgtatact tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta
     6361 gacctgttct ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga
     6421 taacatgaaa gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt
     6481 attaaaaaaa ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa
     6541 tgcacaaaaa tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc
     6601 atcacaatca gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga
     6661 agaactaaga gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt
     6721 aatagattta gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa
     6781 tgtcaatgtc agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga
     6841 ggaacagctt cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc
     6901 agaatcagat ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat
     6961 gctgatcgat gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac
     7021 tgagattgct gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa
     7081 tccatgggaa cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt
     7141 tgccttagaa gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga
     7201 gaaagatctt gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa
     7261 aaaaagaaga gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat
     7321 aatcagttgc acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt
     7381 cttcttcgat tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt
     7441 cttctggctt gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta
     7501 caccggcgaa gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta
     7561 atgattcact tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga
     7621 cagagataac agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta
     7681 ctgggttttc tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa
     7741 tctcatggtc aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga
     7801 tatcttcgcc aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct
     7861 tcaagacgtt ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat
     7921 ctggatgaag tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac
     7981 gttcatccat ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc
     8041 gtccatcttg tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga
     8101 atccggaaag aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg
     8161 tctcaagcgt tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat
     8221 tcggaagggt aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa
     8281 tcgctaatga taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag
     8341 attcttttac cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga
     8401 catctcgtgc gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt
     8461 caatcatttt ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg
     8521 cttcttcttt actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca
     8581 gcgtttgttc gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc
     8641 tcagctcact ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg
     8701 cttctttttt tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg
     8761 attgtgcacc agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa
     8821 gaccgacaat taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca
     8881 tactatcttt ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt
     8941 caatatgcat cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg
     9001 taccccgtag tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg
     9061 atggttttta aaataaatga cactagtttc atataggtaa acctttaaat tgagaaactt
     9121 ttctattaaa ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat
     9181 aattaaataa ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct
     9241 actcttcaga ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc
     9301 ttcttgtcct ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct
     9361 aactagagca gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt
     9421 ttcccgtcct tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac
     9481 aatgtctttt tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata
     9541 catgacatct acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat
     9601 ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc
     9661 caaacggatc gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc
     9721 aaacatcacg cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt
     9781 gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac
     9841 atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc
     9901 gactactacg atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg
     9961 ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc
    10021 atattgcgga tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg
    10081 tacttctgca atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac
    10141 ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga
    10201 gccacttgga attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc
    10261 tttaccatag ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc
    10321 tgccaatacg ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc
    10381 atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt
    10441 tcttaaaagc tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc
    10501 agcacgatct ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc
    10561 gatccatact gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa
    10621 agctacactg taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca
    10681 ttctttgctg actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc
    10741 ttttgtttgg agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat
    10801 gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg
    10861 cttgttttct ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc
    10921 ataaaaatat tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt
    10981 ccctgcctga acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc
    11041 atatggtgcg atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag
    11101 tcgcgattca ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg
    11161 aaaacgttcc tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg
    11221 cggaccggga agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag
    11281 ccctgtacga tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt
    11341 tttggtcatt tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt
    11401 attttgtatg agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc
    11461 tgtcggtcct aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat
    11521 caactcttct aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc
    11581 tagatcagct agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa
    11641 aagttctgtt ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta
    11701 tacgctgtat ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct
    11761 tgtcacagct ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc
    11821 acaatataca taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta
    11881 gacggcaggc tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg
    11941 ctgtttttga tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc
    12001 atgatttgat ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag
    12061 ataatagcaa ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact
    12121 tctccatgtt cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg
    12181 atcgctacta cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata
    12241 actagcattt tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc
    12301 gcacgggcaa tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca
    12361 cctactagct gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa
    12421 ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta
    12481 tttggtaaat tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt
    12541 ttccaccgat attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa
    12601 ttgatctttt tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt
    12661 ttatacctac aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag
    12721 agtaaggttc acattttttg gactctgatt gttttcccct ggattaactg gtacggattc
    12781 tccattcgct ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc
    12841 tgtttccggt aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata
    12901 ggcattgtct actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac
    12961 aggcttggta gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc
    13021 tattgccatt ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt
    13081 ggtctgcgtg ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga
    13141 agaagctgtg tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac
    13201 gacaacgaca atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa
    13261 gcgacttgga tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg
    13321 ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa
    13381 atggtctcca tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa
    13441 tttaccaggc aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat
    13501 ttttgtaatc tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa
    13561 tttttctccg atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg
    13621 aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca
    13681 cagctccttt tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa
    13741 tatccaataa atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg
    13801 gcaggctcac gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca
    13861 aaaaccggca aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa
    13921 cgctgatttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    13981 tccaaccaag ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg
    14041 cagggcagct ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa
    14101 taagccgaaa tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    14161 aagctgacca cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa
    14221 gttgattaaa agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa
    14281 gaataccata aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa
    14341 gacttttcct cataaaatac tggtttattt tggtctcatg taaaactcgc tgaaggcctc
    14401 ttcacttata aatgcttccc cagctgccag tacgtctttc atttggatcg aatcgatgat
    14461 ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt gattggcaat
    14521 atattcgatc gaatttaatg attggaaata ttgtccaagc attttcttct tcagcaaatc
    14581 gagatttgtt tctgacactt ccggatcatc agcgaatcca agaatgattt ctttgacttt
    14641 ttctgctgct ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt gaaattcacg
    14701 atccaagctg aattcaaaac caaagctgtc atcgataatt ccttgattgt acatcgctag
    14761 atagtttcga gaagtattgc ctaaaatcat ttggaacaac agattgattg ctgttttata
    14821 gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat cctttaatac caagtacaaa
    14881 tttatctcgt gtaatggctg cttccaatgc actttgtttg atgatttctt ttgtgttttc
    14941 agggaaataa cgtacaattt cttgctttgg agggaaattt tttgcttctt ggttttcccg
    15001 aataagtttc atcaatttct caggctccat cttaccaact acaaataaaa ccatattgct
    15061 tggctgataa aacgtccgat agcacgtata caagtcttga gctgtgattt tgtcgatact
    15121 ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt tatttagtat
    15181 gccaaaaaac atccgccagt tagggtcgtc ttcatacatt tggatttcct gaccgatgat
    15241 tcctttttcc ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa aatcgatcag
    15301 agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac agatagctgg ttttcgtaaa
    15361 acttgtgaaa gcattggcgg aagctccttg tttcccaaat aattgaaaga catcaccatc
    15421 ttctttttca aacaacttat gttctaaaaa atgggcaatt ccatctggaa ctttcacctt
    15481 ttctttttca ccatagggaa taaattcgtt atcgatcgaa ccataattcg tactgaacaa
    15541 accatatgtc ttgtgatagt catttttagg caacaaatac acggtcaaac cgtttggcag
    15601 tacttcatga taaagtgttt cattgatttg ctcatattct gttttattca tttgcatttc
    15661 ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc agctacttgt tgtatctctt
    15721 tgatcgttac gctctccata cgtttcaacc attcttcatc agataatttt gtttccggca
    15781 gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga gaaagcagat
    15841 attggtttct cagcattgct tttgtctgag acagttccaa ctctgttatt tcaccatttc
    15901 gcaaactttc taattgctcg tgaatcagat gaagtacttg atttcggttc ttttcatcga
    15961 taccggtttg gacactcatg aatcctcgga acgtatccac gctactagag gcataataag
    16021 ctagactttc tttctctctg acattcatga aaagtttaga gtgaggaaaa ccgccaaata
    16081 aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc gtaatacaca tttgtctgat
    16141 aagctaggtt cagctttgct tgaacgattg gctcacgtac ttgtcgttcc ttcacgatat
    16201 tcacttgagg ttgggtataa aacagatctg gataaactgt ttcccgcgac tcaaatggta
    16261 attgcccaac tgcttcagct gctttgtccg gatcaacatc gccgacaaca aaaatgtcga
    16321 tttgatcatt tgccatcatt tcttgatagt aggacgctag cgaactagca gtgatcttgt
    16381 ccaaatcttc gactacgccc aaccctggga tcttttgttc cgggctgttt tcaaaataaa
    16441 gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc cttcaaactt tccaaatata
    16501 gacgcagatt gttcttttcc aagtcaaacg tcaactgatc aaactgatga tttttaatat
    16561 ttggataaaa taaaacctct tttaaaaaat ctaccgcttg agaaaaaagg tttggatcat
    16621 tgacatattc accattcaca aaactgatcc ctgcgtttag ccaatgtaaa tttccttttt
    16681 ttctaactga taatccaaag cttgcgccat aaagctctgc aagttgcgca cttaactttg
    16741 tctgatccga ataattcaaa ctatttgttt ccaacatgct gctgagaagt gaacgtttcg
    16801 tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa aatccggatc gttttgtatt
    16861 gttttgttgg taagacatga agattcacgc cttctgccaa ttgatacacc atatttatat
    16921 gctccttttc acagttgatt gcccgcttac tcttttaatg ggtcaacctg ccttttttac
    16981 ttattcctct gcttgaaatt tataagttca ggcaattcgc cgttttttct aaatgatgtt
    17041 acactttata gtatagcata gatgtttttt atcaaaagaa acagacggct aaaagcgtat
    17101 attgtttttt tattttttat ttttcgatat actaaagaca aataaaagaa gggagggtca
    17161 ttcatgatca aagaattcaa agagttcatc atgcgtggag atgtcttgga tcttgctgtc
    17221 ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc aagtggtgca aggcttgatc
    17281 acaccactaa taggttggtt tgtcgcaatg atcacaggta cttctgattt agaaggagca
    17341 ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat ttgcgtttgg taacgttatt
    17401 agtgctatca ttacatttct gatcactggt tttgtcttat tcttagttgt caaagcggca
    17461 aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg aagtggaaga aactcctact
    17521 gcagaagatt atcttagtga catacgcgat cttcttgcca aacaagtggc agaagacaat
    17581 acacatccag tagagcaaac aaatctttcc gatacttcta aataataaag aatctaaaaa
    17641 aaagaaagcc gattttcagt gtcggctttc tttttttaga ttcttaactt gctacttgtt
    17701 cctttttcat gtcgatatgt ggaatatcat cttccaaata cacatctgag attgccttga
    17761 atccaaacga ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg attggttttt
    17821 ctggaaaatt tttctggatc atgttaatcg tttcttgaac aatctttctt cctaaaccat
    17881 ctttacggtt gtcctgatga acaaggacac gtccaaaatg gatactctcc gcttcttcat
    17941 atactcgcgt gtaggcaagg atcttcccat cagtatcttg aaaaaacgta tgccaagctt
    18001 ctccatcaat gtcatcaact tcttgataag gacactgttg ttccacaaca aaaactgcga
    18061 ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat ttctttacct
    18121 gatattccac taagcaaacg ccccctgctt ttttattctt agcataccga ttcgcacaca
    18181 aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa
    18241 aaattcgatc tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa
    18301 agaattttct tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac
    18361 gccgcggcgt tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc
    18421 gatacgacct tctaaatctt tcttgattga agctaaagtt gttggcatac ag
//
LOCUS       DAZUKD010000053        18277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000053 DAZUKD010000000
VERSION     DAZUKD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.054"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8N9_001553"
     tRNA            12..84
                     /locus_tag="ACG8N9_001553"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8N9_001554"
     tRNA            88..160
                     /locus_tag="ACG8N9_001554"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8N9_001555"
     tRNA            168..249
                     /locus_tag="ACG8N9_001555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8N9_001556"
     tRNA            267..339
                     /locus_tag="ACG8N9_001556"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8N9_001557"
     tRNA            351..422
                     /locus_tag="ACG8N9_001557"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8N9_001558"
     tRNA            439..524
                     /locus_tag="ACG8N9_001558"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8N9_001559"
     tRNA            535..608
                     /locus_tag="ACG8N9_001559"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8N9_001560"
     tRNA            618..691
                     /locus_tag="ACG8N9_001560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8N9_001561"
     tRNA            711..783
                     /locus_tag="ACG8N9_001561"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8N9_001562"
     tRNA            811..884
                     /locus_tag="ACG8N9_001562"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8N9_001563"
     tRNA            898..971
                     /locus_tag="ACG8N9_001563"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8N9_001564"
     tRNA            995..1084
                     /locus_tag="ACG8N9_001564"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8N9_001565"
     tRNA            1097..1170
                     /locus_tag="ACG8N9_001565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8N9_001566"
     tRNA            1189..1264
                     /locus_tag="ACG8N9_001566"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8N9_001567"
     tRNA            1266..1336
                     /locus_tag="ACG8N9_001567"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8N9_001568"
     tRNA            1350..1423
                     /locus_tag="ACG8N9_001568"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8N9_001569"
     tRNA            1448..1535
                     /locus_tag="ACG8N9_001569"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8N9_001570"
     tRNA            1557..1628
                     /locus_tag="ACG8N9_001570"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8N9_001571"
     tRNA            1651..1740
                     /locus_tag="ACG8N9_001571"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8N9_001572"
     tRNA            1753..1826
                     /locus_tag="ACG8N9_001572"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8N9_001573"
     tRNA            1845..1917
                     /locus_tag="ACG8N9_001573"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8N9_001574"
     tRNA            1921..1993
                     /locus_tag="ACG8N9_001574"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8N9_001575"
     tRNA            1998..2068
                     /locus_tag="ACG8N9_001575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8N9_001576"
     tRNA            2081..2154
                     /locus_tag="ACG8N9_001576"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8N9_001577"
     tRNA            2166..2237
                     /locus_tag="ACG8N9_001577"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8N9_001578"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8N9_001578"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4897158.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8N9_001579"
     CDS             3826..5298
                     /locus_tag="ACG8N9_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4897159.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8N9_001580"
     CDS             5381..6169
                     /locus_tag="ACG8N9_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4897160.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8N9_001581"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8N9_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4897161.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8N9_001582"
     CDS             7019..8731
                     /locus_tag="ACG8N9_001582"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897162.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACG8N9_001583"
     CDS             8734..10503
                     /locus_tag="ACG8N9_001583"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897163.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACG8N9_001584"
     CDS             complement(10708..11274)
                     /locus_tag="ACG8N9_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4897164.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACG8N9_001585"
     CDS             complement(11274..12230)
                     /locus_tag="ACG8N9_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4897165.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACG8N9_001586"
     CDS             complement(12230..12886)
                     /locus_tag="ACG8N9_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4897166.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ACG8N9_001587"
     CDS             13256..15274
                     /locus_tag="ACG8N9_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4897167.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /gene="leuS"
                     /locus_tag="ACG8N9_001588"
     CDS             15701..18115
                     /gene="leuS"
                     /locus_tag="ACG8N9_001588"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4897168.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         5592 a         3408 c         4020 g         5257 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttctt
//
LOCUS       DAZUKD010000054        18108 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000054 DAZUKD010000000
VERSION     DAZUKD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.055"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8N9_001589"
     CDS             complement(164..388)
                     /locus_tag="ACG8N9_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS4897169.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8N9_001590"
     CDS             complement(586..2166)
                     /locus_tag="ACG8N9_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4897170.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8N9_001591"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8N9_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4897171.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8N9_001592"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8N9_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4897172.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8N9_001593"
     CDS             5331..5765
                     /locus_tag="ACG8N9_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4897173.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8N9_001594"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8N9_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4897174.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8N9_001595"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8N9_001595"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4897175.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8N9_001596"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8N9_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4897176.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8N9_001597"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8N9_001597"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4897177.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8N9_001598"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8N9_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4897178.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8N9_001599"
     CDS             11001..11471
                     /locus_tag="ACG8N9_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4897179.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8N9_001600"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8N9_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4897180.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8N9_001601"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8N9_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4897181.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8N9_001602"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8N9_001602"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4897182.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8N9_001603"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8N9_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4897183.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8N9_001604"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8N9_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4897184.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8N9_001605"
     CDS             16164..16967
                     /locus_tag="ACG8N9_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4897185.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8N9_001606"
     CDS             17145..18092
                     /locus_tag="ACG8N9_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4897186.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5438 a         3631 c         2964 g         6075 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatat
//
LOCUS       DAZUKD010000055        17161 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000055 DAZUKD010000000
VERSION     DAZUKD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.056"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1001..1666)
                     /locus_tag="ACG8N9_001607"
     CDS             complement(1001..1666)
                     /locus_tag="ACG8N9_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897187.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1846..3828
                     /locus_tag="ACG8N9_001608"
     CDS             1846..3828
                     /locus_tag="ACG8N9_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS4897188.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3832..4470
                     /locus_tag="ACG8N9_001609"
     CDS             3832..4470
                     /locus_tag="ACG8N9_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897189.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4498..4926
                     /locus_tag="ACG8N9_001610"
     CDS             4498..4926
                     /locus_tag="ACG8N9_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897190.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5383..6294
                     /locus_tag="ACG8N9_001611"
     CDS             5383..6294
                     /locus_tag="ACG8N9_001611"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4897191.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6511..6948
                     /locus_tag="ACG8N9_001612"
     CDS             6511..6948
                     /locus_tag="ACG8N9_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897192.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7043..7927
                     /locus_tag="ACG8N9_001613"
     CDS             7043..7927
                     /locus_tag="ACG8N9_001613"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4897193.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8151..11318
                     /locus_tag="ACG8N9_001614"
     CDS             8151..11318
                     /locus_tag="ACG8N9_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4897194.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11386..11883
                     /locus_tag="ACG8N9_001615"
     CDS             11386..11883
                     /locus_tag="ACG8N9_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS4897195.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            11902..12366
                     /locus_tag="ACG8N9_001616"
                     /pseudo
     CDS             11902..12366
                     /locus_tag="ACG8N9_001616"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            12363..13121
                     /locus_tag="ACG8N9_001617"
     CDS             12363..13121
                     /locus_tag="ACG8N9_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4897196.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13207..13947)
                     /locus_tag="ACG8N9_001618"
     CDS             complement(13207..13947)
                     /locus_tag="ACG8N9_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4897197.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14008..15261
                     /locus_tag="ACG8N9_001619"
     CDS             14008..15261
                     /locus_tag="ACG8N9_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4897198.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15583..15810
                     /locus_tag="ACG8N9_001620"
     CDS             15583..15810
                     /locus_tag="ACG8N9_001620"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4897199.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            15933..16259
                     /locus_tag="ACG8N9_001621"
     CDS             15933..16259
                     /locus_tag="ACG8N9_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4897200.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16327..17040
                     /locus_tag="ACG8N9_001622"
     CDS             16327..17040
                     /locus_tag="ACG8N9_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4897201.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         5880 a         2783 c         3423 g         5075 t
ORIGIN      
        1 aaataatttc ttttaaagag ccgagattat cagctatata agagtgaatt tgatgcaaaa
       61 aacagagccg aaacagtctt ggtgttcgta gctctgctta tatgtttcga atttaagaca
      121 aagagttggc aaaagatgtt gataaggaag ataagataac tgtgttatat aaactaggta
      181 actaactgta acaagctaat tttaagagat tcttattaga agacgtttag gaatataact
      241 aagaaaatga ttatacaggt aattgtcatt tcaataatgt tttctaattt tcgcgtcttt
      301 gttttaagaa aaaggccaga atcagttgga aaaaagttcg tacgatagtg aagaggggca
      361 aaatttccta ctaatattcc cactaacaag agataaaacg gccaggttgg tgtgattcct
      421 ctataaaaaa atgcccatgc taccgaaatc ccatagatta acaactgaac aatttttaca
      481 atgaaaaaat tgttctgcca ttactcaaag tctcttcctc cttgtatcgt ttatttgttt
      541 cattttatca ttatgatagg cgcttgaaca atccattttg tattttattg agtactcttg
      601 gataaactac cagcgattat catatttatt tatgttactg taagccatca tataaaagta
      661 aatgagcaga tcatagaaat ataaaaaaga actaaaattg atctaaatta tgtgcagctt
      721 gactacgtac caatctatat tacttactag attggtcgtt aatctattcg gatcgaatga
      781 gcaaaatatt tttgtgaatg tccatccttg attatgattc caaaaagaat cattgggagg
      841 ctgtggcgta aatattatac tataagccag aaatacctat cattctgaac ttttttattt
      901 aggaactttc atctcaaggc tttttcaatt aatttgtacc cctgtttttt ggataagatc
      961 atgtgcattg attttttcta tctctacgtt catctcctac ttaatgccta tctggctcct
     1021 gagggcttct ttttaaggcg tcgtcatagt cttggctgag gatttcttgg taatctgagg
     1081 gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta
     1141 cagcatggtt tttctctttt tctacctctc cagcgtgaaa agacagccat gtaccatggc
     1201 caaagatatc attacatacc caacgttcga tggaattgtc cacgtatcct acagggagtg
     1261 tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt
     1321 attgataatt gataagaaaa agatagacca aacaaaagac gagcagaaag aaagatagtg
     1381 tctttttgag tgagaagggt tgttttgatg aataagattt cttgttaaca agaggttcat
     1441 ctggcaatgt tggttctatt gattcggtag ctgattcaat agaaggaaaa tcaatcggca
     1501 gacctaaagt atgatgtaaa agtgacagca tttcaaggtc tggcattgta cgagaatttt
     1561 cccaactaga aatcgtttta tcggaaacac caattttttc gcctaattct ttttgcgtaa
     1621 gcttacaatt tctgcggtaa gttcgtattt tttctccggt gttcatcttc ttctcctttc
     1681 gattctacag tatatagatt tattctacat gacctataca cttgatagat acgttttttt
     1741 gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt aagggagagt ggtgataatc
     1801 aaaaaaaagt tcacagtttt actcacaatc atagtgaccg gtatcatgag tggttttgcc
     1861 ttggttagta taaataaaca ggtactctat cggacaaatg aatttttcca tcaaacagat
     1921 acttatactc cgattcgatt gccacaacat tttattgggc aagaagaata tgagcagcta
     1981 atcatggtat ttgaaaatgt ctcatcagaa actgagcttt cttatatgaa aaaaacagta
     2041 aatgaatcca atgaagtaga tacatcttgg aacttcaaga tacagccaaa gcttgaaatg
     2101 acattgtatt atcaagggga tgtcaaatct tctcacgaga aggtacccta ttctaatacc
     2161 tcctttcgcg taacgtccat tgaagaatcg atcaaaataa aagattttga aggtgaattt
     2221 ttcttgatga cgagagaaaa agaagcctat gaacggttca tttcactatt cactgatcga
     2281 ttcaatcaag catttcacac tgattatgcc actaatattt ttgaagactt tgaaaatagc
     2341 tattcgtatt cggttatttc aaatacagac gtatataatt atcgagtgta tttaactatt
     2401 ggattgattc ttttattcct tttgttatca tcctttttct tattgatgaa taaagaaatc
     2461 agtttattgt tgaataatga cttttcatta catcaagtat tgtggtgttt attggggaaa
     2521 aagaaaatca ttattacatt cataattgca ggaatcattg gtggttttat ttttatgtat
     2581 tataaggagc aatttcttta tttgttccag atgtacgttt tcttgttcag cggaatatat
     2641 ggtatctctt tgatttctct tttattagtt gaaaaaatcg cttcgttaag agaagcgttc
     2701 aactatatcc aatacattct ttttacattt ggattgttgt tttctcttct tttgaccgtt
     2761 gccaatgtcg aattagcaac tatcgttttt tcttcttcac agttagctgt ttcccatgaa
     2821 ccaccaaaag aaatcgctgg tgaggactat catgtatttt atccagtaac catcgggaaa
     2881 aaccacgtgg attttattta ttccaatcgt tttgcatcag cagcagatca ggaattatat
     2941 gaaactttga ataaaaacgg aagtatatta gtcaatgtat cagattactt gaatgaagaa
     3001 aatgaacagt ttggcagagg gataaaaata aatctgaatt atcttaagaa atttcctttg
     3061 attgatgtct ctggaaggtt gatccaaata accgaaaaag aaacgcaccc tgttatattg
     3121 atccctgaac gatatagaca tacagattta aaaaatgacc tggcgcaaat aacagaatat
     3181 tatcaagaaa tcagtgaaaa agaaccgaaa tttctattta taaaaaacaa tcaacccatt
     3241 tatacattta ttccttctat cccctggatc gaacattatc cggttgttga aatactgaca
     3301 ttgaaaaaca gtatgtattt ggaaagaaat attctcagcg gagaaatcta tccaccgtta
     3361 aagatcaaga taggctcttt gtcgaaagaa aggttatttg agctgattga agaaagtcag
     3421 ttacgtgata acttgcagtt atcatttccg tatactcaga ctgaagagat agctgtcaaa
     3481 gtgttaagca gatcatttca ttacttgata cagatatttt tactcacttt cttttctttc
     3541 ttcttgttga gctatgtcat gttatgcata tattttgtct acaattgtcg gaaaatcgct
     3601 atctttcgtt caagtggtta ttcgctcttt gaaacgtata aagatttctt catgatgaac
     3661 ctcatcaaat ggggaacgac aagtgtaatc ttcctttttc ttatagaaag agagcctaaa
     3721 tatcttttta atattttttt ctttagcttc attgatttta ttctttcagt tgtttttatt
     3781 ttgtctacag aaaagaaaag tcagttattg ctgatgaatg gaggttaaca attggagccg
     3841 ttgatagtga taaaaggtgc gaaaaaaatc attcgaaaca aaacgattct tcatttggat
     3901 cgtttcactg cggatgctgg ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa
     3961 agtacgttat tgaatatatt aggattgaat gatcagttca gttcaggaca attctatttg
     4021 ttcgatcaag agacgaaaca attaactggg agagagaagc gcttgataaa aagaaatacc
     4081 atatcttttc tttttcaaga ttttgggttg attgaatatg aaacaatcga ttttaattta
     4141 gatattggat tgaagttttc gaatttaaat gtttcagata agcgaacagc aaagtatgag
     4201 gcattgaaaa aagtaaatat aaacaagaag ctaagtacgc ctatctacac attatctggt
     4261 ggtgagaagc aaagaatcgc ctgtgcgaga gtcttattga agaatagcaa aatcatttta
     4321 gccgatgaac cgactgggtc attggataaa agaaataagg ataaagtagt tgaaatctta
     4381 tcgcaacaag cttcccaagg tacgttagtt atcgtagtca cgcatgatcc atatctgacc
     4441 aaaataggaa acaagaagat catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg
     4501 aaaaaagcag ttattatttg tgcactaggg ataggttttg ttggaggaaa cgctatgtca
     4561 tctgaagtgg aagcaaattc agttcacggt gaaatctatt acaaatcttt aaagccagga
     4621 gagattcatt ttgatgctaa aggtaatatt attaagtcaa atttaccagt agaccgggaa
     4681 atttctagaa cagcaattaa tcatagctct ggaagatggg tttattatag tgatacaact
     4741 tgtacgagaa aaacaggtca ttctaaccac tatagtcgtg tctataatcg tcacggtgca
     4801 aaagcgaaag ttggaaactc tattcgatca gcaaatgcaa caaaaggagt atgggcctat
     4861 gcaagtgcga caggaaatag aactgacaca ttctcatgtt ggtacaatcc aaccggttgg
     4921 tattaagtat ccaaaagttt atataaaata gaaaaaacat ataatgaaac agcgttaaaa
     4981 gttatttttg gtagttttaa tatattcatt tttagttgta taaaaatata tcataataaa
     5041 aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca
     5101 gcggaagaag aaaaacaaaa tcgataacat ttgttctaag aattagttac gttaaatacg
     5161 gaacgcaaga tagacttgaa aaacggtaac aaaagaagta gtatattaag gtgtgatgtg
     5221 ctacttgaca tttattctgc tgagcgaact ttctggattg tgtagcctta tatctattca
     5281 ggacgtatcg agcagattag taaaggtgtt cttgatgatt ttcatcttga acacctttgt
     5341 ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat aaatgaaaaa aagattttta
     5401 gcacttgcca tcgtactagg cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa
     5461 acagccgtta gttataaagg cggtacaatc agcgaacaag aagtaatgga tagcttgaaa
     5521 aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg tttatcaagt atttgaagat
     5581 aaatatggag atgacgtatc aaccaaagaa atcgattctc aatatgatca aacaaagaaa
     5641 caattgggtg attcatttga ttcacaattg aaatctgcag gttacacaga acaaacattc
     5701 aaagacagta tcaaacaaag tctagccttt caagaaggtc taaagaaaca tatcaaattg
     5761 accgatgaag atttaaaaac ggcttgggaa tctttccatc cagaagtgga agctcaaatt
     5821 atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga aagctgctga taagggagac
     5881 gatttctcta aattagccaa agacaagtca actgatacta cgacaaaaga agatggcgga
     5941 aaagtgaaat ttgattccac tactacaacg gttccggcag aagtcaaaga agcagccttt
     6001 aagttgaaag atggtcaagt ttctgatgtg atcacttcta ccaatgcttc aacttatact
     6061 acggaatatt atgtggttaa aatggtgaaa aaccaaaaca aaggcaacga catggataaa
     6121 tacaaaaaag aattgaaaga aattgcaaca gacacaaaat taagtgacag taccttccag
     6181 aataaagtaa ttggcgaagt attgaaagac gctaatgtaa aaatcaaaga caaagacttt
     6241 gaaaatgttt tgagtacatt cacttcagat tctagtacag caagtagtaa ataagcggta
     6301 taaataaaaa atatgaacta tttaaatcaa taaaactgtt catctaaatc tttttattag
     6361 aactaaatca acagtagttt aattactact tgatttagtt ctttttattt taaaaatttt
     6421 ttttacttag aatccagcga ccctagatat ttttctgtat aatcttaaat aaagaaaaca
     6481 atatcaaaaa atgcacgtag aagtagataa atggagtggt tttgcaaatt tcttttattt
     6541 ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc aggaagtaca
     6601 atggatcatt tgtatataaa atatattcat caattttccg ttttggtgtt aggcacacta
     6661 attatagcgg ggattgtcgc atttattatt caaggccgtg acatattaac agtacatcaa
     6721 gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga taacaaaaag
     6781 tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc acctaagatt
     6841 tttactttat taggtataca atgcctatca ttttctttta tttcctatat cattgtttta
     6901 attttcctca gtttcatagt atggatgttt tatcttgtca aaaaataaat gaatgtttca
     6961 tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata ttaaagaaat
     7021 caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc ccagttcagg
     7081 cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat cggtgtttga
     7141 tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta ctcgcgaagc
     7201 ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag tcaacgaagg
     7261 aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc cattgggtac
     7321 ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag cgatcaatca
     7381 tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg actattttat
     7441 gaatgtcgtc gcaattggct ctatccctga atcaatcaat gatgtagatg cggagaagaa
     7501 gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag cttccacaca
     7561 atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga gcagtacctt
     7621 gctgatagga ctaactaact cagtcggcgg ttttgaaaca ttacttccaa atgcaaaagt
     7681 ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg atacgcttaa
     7741 aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag cctaccttac
     7801 ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga atgttgatgg
     7861 agatgaaggg gataaattac ctgtgacaat caaagtattg ccctcacatt tacaggttta
     7921 ttgttaaaat ggaggttgtg accgaggagt tggcgggaga acaccgcttg ttcgggataa
     7981 gggactgtga caagactttt gtcacagcct tttttttgta caaaaaagaa atttacacct
     8041 aatagaaaaa tttttccata aaaatacata ttttaaatat gaaagtacaa ttggaaatag
     8101 attaaaacga taaaataaaa ttgtcaaaac gtcagagagg atggtggaga atgaatcaat
     8161 gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg gtgacatccg
     8221 tttttgccga tgtagagaaa acaaacgaat caacgcaatc agagaatcaa agttttgaca
     8281 agactacttt ttcagaagaa tcgattactt ctgaaactac agaatctcaa agtgaggctt
     8341 ctactaataa aaggcaagaa gaaaataaga caagtgagac ctcagaagta gctgaacaaa
     8401 cgtctgatag cagtgaagcg gcactggcaa gagctgtttc agcggatggg tacagcgaag
     8461 ctgccacaac agaagaactg gcgcagcttc tcgcagacga atcagtaact aagatccgac
     8521 tgattcagcc tttgacttta gacagggaat tagagatcaa acgagatatc gttattgatt
     8581 ttggtggatt cgctcacaat ttcggcacac accatattta tatcaatgag atacctcagt
     8641 cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc aggtggattg acaccaaata
     8701 tcgatggaaa tgccattatc attgcttata tgaccgattt ttggggaagc agatatcatt
     8761 ttacgggaga aataaaattt accgggacac ttgatttgta tgatgggtcc aaattaggac
     8821 tgatttacgc tcctcgtgca actgtcacat tagatggtgt ttccggtgtg ttggatgtcc
     8881 aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc ctatttttgt agaagctatc
     8941 aattgaatgt gatcaatggt agtcaacttt atggaccgta tctagggaaa ttctacggtt
     9001 ttctagattc aggagatgag actggtagtg gaaaaacagc gccaggaatc catatcttaa
     9061 gcggttcaaa ggtctctctt gattatgatc gggcagatgg cgttagcgat gaaggagagg
     9121 cagtcgatac gctacctggt aatgtcatgt ttgaagtcag tggttcggga agcgaatttt
     9181 cggtaaatac aaagatcaat attaccaatg acaacaatcg cggaatcatc caaatgagag
     9241 gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat caccatcaat acagcaacta
     9301 cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa
     9361 tctcggtcaa aatggctgga gatgacgatc aacccgggaa caatgggatg cgttttgtag
     9421 gaaaaggcct ttctttgatc gtagaaggag ccggatcagt tatcgacatc gataagcaga
     9481 gtgggcgttc cagcgcagtt cgttttgaga atggtacgca aaagctgaca gtaaccaatg
     9541 gaggctcatt gagggtaaga aatacaggtg atggcaaatc ttacgtgaac agactcaatc
     9601 aaggaaatca agcgatccaa ttttatgacg cttctggttt tctagaaagt ccaggttcag
     9661 ctgattttac agtcactgga gaaaaatcaa atatcgatat acgcgcagat tccggagcaa
     9721 ctgtagatac aactggagat attgatttaa attttacggc aggtgaaaaa acttatttag
     9781 tcatggtcgg gaaaacaggg gaagataaga ccggcatcat cagtggggat gtgttgaatg
     9841 tggacttaga atctcctacc tattttgatt tccaaaacaa acggattgga agcgagacca
     9901 atggcatagg cggctgggta ttccaaggaa atggagattc ttctttaaag atcgcccatt
     9961 cagaaatcgc tttatggcga aaaacaggcg caacattcga taactttgaa aaagacccgg
    10021 attatttttc tgatatgaca gacttatcga tcaatgggac cgacttaggc aatttcgtat
    10081 caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga atgaaaaatt
    10141 ataatcgaat cagtgccaat aatcagctcc cgatgattga atccttaaga gtgccaacga
    10201 atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa
    10261 gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttacgca gatacgacga
    10321 aatcatctgt agaaattact gctctcacgc aaggacaaac agatgaaaat gaaggggcag
    10381 gatataaccc atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg
    10441 atgagagatt aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg
    10501 gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt cctcctgaac
    10561 ctgctaattt aagtaccaca ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg
    10621 aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg
    10681 atgatacagg ggaatttgtt ctttctatcc cagaaaatca actttttgca ggagatgtat
    10741 tagaaattgt attaaacgac caagcaggag aagctactgc taagggtgtg cagaacaagc
    10801 caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt gtttatcatg
    10861 atgccccgcc ttttgcacct gctacgaaga tcgtagtaga aggtggtctc tcatttcttg
    10921 ccccttccaa actcgatttt ggtcagatca aagcaactgg tttgacacaa gaagcatttg
    10981 gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaacg aaaaatccgt
    11041 ggatcttaac gataaaatta tctcagcctt tcacaaatga tgcaggattt tctatgcagg
    11101 atgtcctata tttcaagaaa ggagaggagt atcaattagt gaatgaagaa gaactggaaa
    11161 ttgtcagagg acaaaattca acgaatgtgc aagcagatta ttcagaatat ttaaataacg
    11221 gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat tactcagcag
    11281 agataacatg gacattagca gatgcgccga cagaataaag atcaaggagg atgattgatg
    11341 aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttatgat ccttctcctt
    11401 ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc ggtagatttt
    11461 gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag gatggagcct
    11521 gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc agtaacagtt
    11581 gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata tggagaatca
    11641 aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa aacagtggga
    11701 gaaataactg ttcctcctca aagtgcggtc actgagacac tttcactgaa agcgccagaa
    11761 aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt
    11821 tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt
    11881 tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa aaaaagctta tgcggatcaa
    11941 agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc ttatatgttc
    12001 ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta cctttcaata
    12061 aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt cccattagaa
    12121 ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg gaaagaaaaa
    12181 cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca attgaatgaa
    12241 aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct gatacttgtt
    12301 ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag aaggaaaaag
    12361 aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta ttatgtacac
    12421 aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt tctgtgacca
    12481 aaccagatgc tgcaaatcct gaagacgttg ttccagttcc tcctgttgat ccaaatgatc
    12541 ctgatactcc ttttacacct gacgaaccaa atcagggaac atccggatta ctgacgattg
    12601 accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa acagtatatg
    12661 ctgcattaca aagcggaacg gacagtactg gagcacaaac acaagtacag aatttcgtac
    12721 aagtaacgga taaaagcgga aattatgccg gttggaaatt atcagtcaaa cgaacagaat
    12781 ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa ttcaaaaacg
    12841 ccgtgattcg aaatagcgca actaatagca gtggaatgcc atctaccgtt tctggaactg
    12901 gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga tctagtaact gcaggagcaa
    12961 atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa gggagacaat
    13021 cagtggaatt atttgttcct gtatccaatt atgctgtaga caactacgtt tcaacattca
    13081 catggtcgtt aacagatgct cctacagaag aggaagcgta aaatcatatc aacgatttca
    13141 gggagtggag ccgattcaag gcccactccc tgaaatctgc tatgcttacg tctcagctct
    13201 tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa
    13261 gatctttcga ccaacgagta aggagtcacc gttgaaaaat tctaacacat aatgagcaga
    13321 atcaattttc tgcaattgcg ataagttgat acagtaagat tttaaaagta aaaagtaagc
    13381 atacttttct aactctgtct tcaattgttt taaagaagta ttcactaatt tatcttctgt
    13441 agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat ataaaatttc
    13501 ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat cgacatttac
    13561 gatctgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt cagataaatt
    13621 cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct ctactgcaaa
    13681 attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac gtatttgttc
    13741 ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga aaacatcgtg
    13801 attatcaata gtgcccaact ggatcaactg attgatttga gagatagaag tgagctgcac
    13861 gatattctcg atatgatgat tgaccaattc tctgatgatc cattcgctgt gaaactggtt
    13921 atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat atgtgtacat
    13981 tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta tttgaccttt
    14041 actttgattt tagatttagg tatctattta ttcttttcga aacaactgct agtaaaaaac
    14101 agctttggtt gtatagcttt gatggtggtg acacttttac ttcctatccc taaagtgagc
    14161 tttccttacc tagttggctt attattgatg tttctttttt attttactac gaaaaacctg
    14221 tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag cttttttctg
    14281 gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag tttttttgca
    14341 ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca gacgagattt
    14401 ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg tattttatta
    14461 agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc taattatctg
    14521 cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc cattatgttg
    14581 atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta tcaaataaat
    14641 gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct actgttttca
    14701 ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga aatattagaa
    14761 gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa tattgtatcc
    14821 ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg
    14881 atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt tgtttttatt
    14941 cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga tcagagtccg
    15001 attacgatca atctgctgga agaaaaagag ggatcacgtt ttagtatagc gaacaaggca
    15061 aatccaacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat
    15121 ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta
    15181 aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct ggcatctaga
    15241 aagagcaaat cagcctactg acttactgtc aaaggataag tttgaggaaa gtctacacgg
    15301 agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac cgttgtcacc
    15361 cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct acttagtatt
    15421 agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg aacccacgat
    15481 cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata tcgcttattt
    15541 taggcgtgga gaagcaagca aatccctacg atcacaaaaa aaatgctgac ccagcagtta
    15601 agagagttag aacaagatga aagtatccat cgaaaagcct ataaccaagt cccgcctaaa
    15661 gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt agcgttgtct
    15721 gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga cacgctactg
    15781 catacacatg gtggctacct aaattattga ttttctatga catttatttt aaataaaaga
    15841 aaacaggagt ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg tatgattaat
    15901 caagtaagaa gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt
    15961 ttagagatga acgtaattgg cgacaatttc ataacgaaaa agatttggct atctctattt
    16021 ctttggaagc atctgaattg ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag
    16081 aaacacaatt agaacgaatc aaagaagaac tggcagatgt attgatctat tcatacatga
    16141 tggcagacaa ccttcacctg gatctagatg agatcattga agaaaaattg atcaaaaata
    16201 atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga
    16261 aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga
    16321 gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct cttgttcctc
    16381 ttgttattag taggactagc tttgattttc aatgaacaga tcaaggatta ttttgtcaga
    16441 gagacaggag ataaatatgc tatagcgaat gtaacaaaag aagatctgaa aaagaataac
    16501 gataaagatg tcagttttga ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg
    16561 aggtcgcaaa tgagaggaac agatctacca gtgattgcta gtatcgcagt cccttcagtt
    16621 tcaatcaatt taccggtttt caaaggcttg gataatacgt cactacttta cggagcagga
    16681 acattatctc cagatcaaga aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca
    16741 acgaaccctg aattgctgtt tacaccgcta gaaaatttag agatgggtgc caagatctat
    16801 ttgacagatt tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa agttgctcct
    16861 acagataccc aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt
    16921 ggcgacatgg acgccgtcac gcgactagtt gtacaaggag aactggaaag tgttacatcg
    16981 ataaaagatg cgacagatga tatgagatct gctttcaatc tagagacaaa aacattttag
    17041 gatactggga cataagtcgt tacagattaa aaacagccaa aggatatgaa tcgataagga
    17101 aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg catctctgat ttggttgttt
    17161 t
//
LOCUS       DAZUKD010000056        16524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000056 DAZUKD010000000
VERSION     DAZUKD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.057"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8N9_001623"
     CDS             complement(24..722)
                     /locus_tag="ACG8N9_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4897202.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8N9_001624"
     CDS             complement(715..2067)
                     /locus_tag="ACG8N9_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4897203.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8N9_001625"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8N9_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4897204.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8N9_001626"
     CDS             3851..4588
                     /locus_tag="ACG8N9_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4897205.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8N9_001627"
     CDS             4713..5192
                     /locus_tag="ACG8N9_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4897206.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8N9_001628"
     CDS             5189..5956
                     /locus_tag="ACG8N9_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4897207.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8N9_001629"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8N9_001629"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4897208.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8N9_001630"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8N9_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4897209.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8N9_001631"
     CDS             7567..7797
                     /locus_tag="ACG8N9_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897210.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8N9_001632"
     CDS             7788..8036
                     /locus_tag="ACG8N9_001632"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4897211.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8N9_001633"
     CDS             8142..8726
                     /locus_tag="ACG8N9_001633"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4897212.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8N9_001634"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8N9_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4897213.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8N9_001635"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8N9_001635"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4897214.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8N9_001636"
     CDS             10627..10806
                     /locus_tag="ACG8N9_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897215.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8N9_001637"
     CDS             10828..11853
                     /locus_tag="ACG8N9_001637"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4897216.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8N9_001638"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8N9_001638"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4897217.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8N9_001639"
     CDS             13425..14372
                     /locus_tag="ACG8N9_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4897218.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8N9_001640"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8N9_001640"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4897219.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8N9_001641"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8N9_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897220.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8N9_001642"
     tRNA            15637..15709
                     /locus_tag="ACG8N9_001642"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="ACG8N9_001643"
     CDS             complement(15828..16466)
                     /locus_tag="ACG8N9_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4897221.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5636 a         2949 c         3323 g         4616 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaa
//
LOCUS       DAZUKD010000057        15999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000057 DAZUKD010000000
VERSION     DAZUKD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.058"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1409
                     /locus_tag="ACG8N9_001644"
     CDS             <1..1409
                     /locus_tag="ACG8N9_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4897222.1"
                     /translation="NTNVSTEIMAGVTTFFAMSYILFVNPTILSASGMPFQAVFLATI
                     IASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAMVFICGLINIF
                     ITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQSAISSSVVEGG
                     KATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIGIVATTVLGIF
                     MGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQVLMTIIAFSL
                     SDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIATSVGAIFGTS
                     NTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATAPALILVGVMM
                     MASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVIKGKYNQVSVA
                     LWIVDILFIINFIILATI"
     gene            1579..2775
                     /locus_tag="ACG8N9_001645"
     CDS             1579..2775
                     /locus_tag="ACG8N9_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4897223.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(2837..3022)
                     /locus_tag="ACG8N9_001646"
     CDS             complement(2837..3022)
                     /locus_tag="ACG8N9_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4897224.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            3253..6567
                     /gene="dnaE"
                     /locus_tag="ACG8N9_001647"
     CDS             3253..6567
                     /gene="dnaE"
                     /locus_tag="ACG8N9_001647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4897225.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            6735..7697
                     /gene="pfkA"
                     /locus_tag="ACG8N9_001648"
     CDS             6735..7697
                     /gene="pfkA"
                     /locus_tag="ACG8N9_001648"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4897226.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            7771..9555
                     /gene="pyk"
                     /locus_tag="ACG8N9_001649"
     CDS             7771..9555
                     /gene="pyk"
                     /locus_tag="ACG8N9_001649"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4897227.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(9590..10492)
                     /locus_tag="ACG8N9_001650"
     CDS             complement(9590..10492)
                     /locus_tag="ACG8N9_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4897228.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            10678..11247
                     /locus_tag="ACG8N9_001651"
     CDS             10678..11247
                     /locus_tag="ACG8N9_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4897229.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            11305..11484
                     /gene="rpmF"
                     /locus_tag="ACG8N9_001652"
     CDS             11305..11484
                     /gene="rpmF"
                     /locus_tag="ACG8N9_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4897230.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            11654..13075
                     /gene="gndA"
                     /locus_tag="ACG8N9_001653"
     CDS             11654..13075
                     /gene="gndA"
                     /locus_tag="ACG8N9_001653"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4897231.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            13268..13954
                     /locus_tag="ACG8N9_001654"
     CDS             13268..13954
                     /locus_tag="ACG8N9_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4897232.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            13951..15456
                     /locus_tag="ACG8N9_001655"
     CDS             13951..15456
                     /locus_tag="ACG8N9_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4897233.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5198 a         2767 c         3390 g         4644 t
ORIGIN      
        1 acaatacaaa tgtatcaact gaaataatgg cgggagtaac gacattcttt gccatgagtt
       61 atatcttatt cgtaaatccg acgattttgt cagcatcagg tatgcccttc caagcagtat
      121 ttctagcgac aatcattgca tcgattattg gtacgctggt aatgggattg ttcgcaaatg
      181 taccatatgc tcaggcacct ggtatgggat tgaacgcatt tttcactttt acggtcgttt
      241 ttgggcttgg ttattcttgg caacaagcct tggcaatggt gtttatttgc ggattaatca
      301 atatttttat tactgtcaca aatattcgaa aaatgattat tcgggcgatt cccgaaagtc
      361 ttcaacatgc gattggtggc gggatcggta ttttcgttgc gtatgtcgga atcaaaaatg
      421 caggattctt atctttttca gcagaccagt cagctatttc gagctctgtc gttgaaggcg
      481 gaaaagcgac aaatgttacg ataaatggtg gaatcgtccc tgcgttagcc aactttgaca
      541 acgctcctat cttattggct gtgattggtt tggtactgac agccatttta gttgtaaaaa
      601 atgtccgtgg ggctattctt atcggtatcg tagcgaccac agtccttggt atttttatgg
      661 gtgttgttga tttaagttcc attgattggc aaacgaattc gttaggtaat tcatttaaag
      721 aattaggaac aactttcggt gcagcatttg gttctgaagg catgcaatct ttattcagtg
      781 attcttccaa gattcctcaa gtattgatga cgatcatcgc attcagttta tctgatactt
      841 tcgatacgat cggaacattt atcggtacag gacgccgcac aggtattttc tcaaaagaag
      901 acgagatcgc gttggaagat ggtcgtgggt tcaaaacaaa aatggataaa gcactatttg
      961 ccgatgcgat cgctacttct gtgggggcta tctttggtac atcgaataca acaacttatg
     1021 tcgaatctgc agctgggatc ggggccggtg gacgtactgg tttgacttca gtagtagtgg
     1081 cagtgctatt cgcattaagt agtttattct ctccactgat cgccattgta ccaaatcaag
     1141 caactgcgcc agcattgatt ttagttggag taatgatgat ggcttctttt gcagacatca
     1201 aatggctaga tatggaagaa gctttaccgg ccttctttgc atcgatcttt atgggattgt
     1261 gctatagcat ttcctatggg attgcagcag gatttatttt ctatacaatt gtgaaagtaa
     1321 tcaaaggaaa atacaatcaa gtttctgttg ctttgtggat cgttgatatt ttattcatta
     1381 tcaacttcat cattctagca acaatttaac taaagagaag ggattggaac agtatttgtt
     1441 ctgatctctt tttgttttac ataaaaagaa cgttacatat aaaatgacct ttgcgggatt
     1501 tttctctttt tactggcgag atcgttgctt gtttattcga aaataggtat gataaaagag
     1561 acgaaagagg gatgatagat ggagattaat tggagaaaaa atttagtgat tgcgtggatt
     1621 ggctgttttt tcactggtgc aagtattagt ttagttatgc cattcatacc agtttatgtt
     1681 gagcaactag gaacaccaaa agatcaaatc gaattgtttt cgggactagc aatctctgtg
     1741 actgcttttg catctgccgt ggttgcacca atctggggaa atctagctga tcgaaaaggc
     1801 cgaaaactca tgatgatccg tgcagcagca gggatgacat taacaatggg atctttggct
     1861 tttgtaccaa acgcgtattg gcttttgatc atgcggtttt ggaatgggat tttgtctgga
     1921 tatattccaa atgccacagc gatgattgct tcacaggcac ctagagaaaa gagcggatgg
     1981 gcgttaggaa cattgtctac cggagcgatt gctggaaatc tgatcggacc ttcaatgggc
     2041 ggtgcactgg ctcagtggtt tggtatggaa aatgtattct tgatcacagg ggctttgttg
     2101 atgatcacaa cggtattgac cattttctta gtcaaagaag atttccatcc aatcgagaaa
     2161 aaagatctga taagcaccaa agaaattttt tcaaaaatgg atcatctctc tattttgatc
     2221 ggtctcttca ttacgacctt gattttacag ataggaatca ctacgatcag ccctattttg
     2281 accttatata ttcgtgaatt gagcggaagt acagaaaata tcctttttgt cagcggcttg
     2341 attgtttcga tagctggtgt ttccgctgtc ttttcttcac cgaaactagg aaaactaggg
     2401 gataagattg ggaatcaaaa agttctaatt ggaggactca ttttatcatt ttgctgttat
     2461 ttacctatgg cattcgtcac tacaccgctc caattaggta tacttcgctt tattttaggc
     2521 ttttcaactg gcgctttgat gccttctgtc aatacactga tcagtaagat tacgcctcaa
     2581 gaaggagtca gccgaatcta tagctacaat cagatgttta gcaactttgg acaagtttta
     2641 ggtcccatgc taggatcgac agtcgcgcat gcttataatt acagtgctgt ttttattgtg
     2701 acaagtttgt ttgttctgtc aaatattctt ctttctttat tcaactttcg taaagttctt
     2761 cgcagcagat tataatggtg gtgaggagta gaaaataaaa aagagcttga gaaatctcaa
     2821 gctcttggct gtttttttac gaatgggcag aggtttcttt tcttggcgca actgcaccaa
     2881 tcaaaaggat aatgacaccc gcaatgaatg aaatgattgt tgtcaaagta aaatcatatg
     2941 ttccgcttgt taatgcagca ccaatataac cgactacttg acctagaacg acagcccaga
     3001 ataatgtaac gatatatcgc ataaagaaca cctcttttct atcttaattt taccagattt
     3061 tctttgaatg taaagaaacg agaccaaatc tcaataaaat ttgagttctt gcgaaagaac
     3121 tcattcctac agattacaag caagcaaatg aagtggaata atcaacttca gcgcctgcta
     3181 tcttaatgaa agtaaaagga tcaaaccttt taacgctagt gaattttaac gaaaaaaaag
     3241 gcggaatgca agatgttttt cccacaactt tatacaatta cttcttattc tcttttacaa
     3301 agtacgatac gtattcgaga atatgtacaa gaagcaaaaa aacaaggcta cacttcatta
     3361 gccataacgg ataaagatgt cttatacggt gcacctgaat tctatcgtat ttgtgtagaa
     3421 gaaggagtac agccaatcat tggattacat cttagttata cttatgaaga aaaaaattat
     3481 ggcttgcttg cgtacgcaaa agatgaaata ggttatcagc agctgatgaa actttccagc
     3541 cgaaaaatga tccaagaaaa attggaactt gcagaaatga cagaaaccga ccatctagcg
     3601 tttgttgttc cggcaaatca ttctgtttct gaggctttca ataaagagga taactttttc
     3661 ggtcattggc agtttttact tgaaaatgtt tttactagaa atttttatat cggcttgaca
     3721 gaaaacacgc ctgatcaatt gcctgaatgg tatcagtatg tgataaaaga aaattattcg
     3781 ctttgtgcat tacatcccat cgctagttta caacaagaag aactttttgc acttgaagtg
     3841 atgaaacata tcaaagaggg aagccaaata cctttagaaa atttatctgc atctttacca
     3901 ctaacagcaa atgtctatct tctttcagaa caagaaatga tcaaacgatt cgaaaaagcg
     3961 ggtctttccc aagctgtaat aggcgcccag cgtttagctg aatcgatttc atttgaattc
     4021 cagttccatc agaaattact tccgcattat ccagttccta atggaaaaca agctggagaa
     4081 tatttaagag agttatgcta ttcgcaagtg gaacgccgaa tcactgacta tacaaaggaa
     4141 taccaagaac gtttggacta cgagttatct gtcatccatc aaatgggatt cgatgattat
     4201 ttcttgatcg tatgggatgt aatggctttt gctcatagaa acaaaatcgt taccggtgca
     4261 ggtcgtggtt ctgctgcggg ttcattagta gcctatgtgt tagaaatcac agatgtggat
     4321 ccattagaat atgatttgct gttcgaacgc tttttaaacc ccgaacgctt tacgatgcca
     4381 gatatcgatt tggatattcc tgacaatcga cgtgaagaag tattgaatta tgttcgagaa
     4441 aagtatggac aatatcatat ggcacaaatc gcaacatttg gcacaatggc agcaaaaatg
     4501 gttcttagag atgcagctag agtgtttggt ttatcgcaaa gtgaagcaaa ccgctggtca
     4561 aaagcaatac ccaatcaatt gaaaatcaca ttgacccaag cttataaaac ttcgcatcaa
     4621 ttagtagaat tagttcaggc aagcgaaaaa aaccaacttt tattcaaaac tgcagtgatt
     4681 ttagaaggat tgccaagaca tgtttcgact cacgcagcag gtgtagttat cagcgataag
     4741 aatctacttg aactagttcc gctccaacgc ggttctgatg aagcttttct gacccagtat
     4801 acgatgaatg aggtagaagc aatcggtctt ttgaaaatgg actttttagg actacgcaac
     4861 ttatcgatta ttgattttac tttgaaagct gttaagcgag tagaagggta tgagatccga
     4921 ttaaaagaga tcccgctaaa tgatgcaaaa acgctgctct tgtttcaacg aggcgaaacc
     4981 tcaggagtat ttcaatttga atctgctggt atccggaacg ttttacgaag attagatcca
     5041 gaaaacatcg aagatattgc cgcagtcaat gcattgtatc gaccaggccc tatgcaaaat
     5101 atagatacct tcattgccag gaagaaagga aaagaagcca ttcgctaccc agatgattca
     5161 ctgatcccga ttttgaaaaa cacatatgga gtgatcgtct atcaggaaca aatcatgcag
     5221 atcgcctcga ctatggctgg atttacatta gggcagtcag atattctgcg acgggcagtg
     5281 agtaaaaaga aaaaagacgt attagatgaa gaaaagtcgc attttgtatc aggagcatta
     5341 caacagggat attcggaaaa aacagcagcg accgtatatg aatatatcga aaaatttgca
     5401 aactatggat tcaatcggtc acatgcattt gcttattcat tcgtgggatt ccaaatggcg
     5461 tatctaaaag cccatcatcc aggtgctttc tttaccagcc tgatgaattc tgttcggcat
     5521 aacacatcaa aattgaaaga atatattgca gaagcacgta aaaataaact caaactttcc
     5581 tcgccgtcca ttaatcaaag taattccagt tttgagttgg tcaataagga agaaatcaga
     5641 ttcggactga cagcgataaa aggtattcgg cgtgattttg tagaagatat attaaacgaa
     5701 agaaaacaaa atggcccgtt ccagtcagtg gatcaattcc tcatacggat cgataagcgt
     5761 tggctgaaat tagagttact gcaatcactg acagcagtag gagtttttga tgaattatct
     5821 cctaacagga aacaattgat gcttgatctc gaaggaaaaa tccaaaatat cgtttacagt
     5881 ggtggaagtc ttgacttgct gggaatcatg gcattaaaag aagaagaagt cgctgactat
     5941 acactagaag aacgcttgaa gctggaagag gagtacttag gtatttattt gtcaggtcat
     6001 cctgcagaag ggtttgatcg tcttcgttta gctaaacaag ttcagctgat tacagatatc
     6061 gtccctaatc aaccagtcaa tttattagtg atgatcaagg aagttagaga aattcggaca
     6121 aaaaaaggag aactgatggc ctttgttgaa ggaaccgaca gtagtggaga aatttctgtc
     6181 acagtttttc caactcttta tcgtaaatat cgtaatttgt ttgccgttga tcgagtgatc
     6241 tttgttcaag ggcggagcga aatcagcaaa tataatcagg aactgcaact aattggagaa
     6301 agctttgctg atcctaacca gttagaagcc cagtatcctt cacaaacttg ctatttgcgt
     6361 attacagaag aacgggaaga tcccgagata atgaaacaat tacaagtgat ttttcagaaa
     6421 cacccaggat atataccagt agttatgtat cacgaaaaaa cgcaacgaaa agtagttctg
     6481 tcagatgctt attgggtgga tgacagcaag agtataaagc ggcaacttat ttatctgctt
     6541 ggcgaagata atgtggtttt caaataactt acggtaaaaa atactacttg ctttcatttt
     6601 ttttcatttt tttatccgta aatcaagaca agtgagaaaa ataatgttag aatgttagtc
     6661 gtggttaacg caaaaatggt tgtaaaccta agtcatactt aggataaagt attttaatga
     6721 ggtgaatgtt tattatgaaa cgcatcggaa ttttgaccag tggcggagac gcacctggaa
     6781 tgaatgcagc tgttcgtgcc gttgttcgta aaggtattta cgaaggactg gaagtatacg
     6841 ggatcaatta tggttttgcc ggtttagttg ctggagatat tcgtcgtctt gatgtggcag
     6901 atgtaggtga caagatccaa cgtggtggta cattcttgta ttctgcacgt tatccagaat
     6961 ttgcaactga agaaggtcaa ttaaaaggaa tcgaacaatt gaaaaaattc ggaatcgaag
     7021 gtctagtggt tatcggagga gacggctctt atcatggcgc aatggctttg acaaaacgcg
     7081 gtttcccagc agtaggtatt cctggaacaa tcgataatga tattcctggg actgatttca
     7141 caatcggttt tgatacagca atcaatacag tactagaatc aattgaccgt attcgtgaca
     7201 cagcgacttc tcatgttcgt acattcgtta tcgaagtaat gggacgcaat gcaggagaca
     7261 tcgctttatg gtcaggtgtg gctggcggag cggatgaaat catcatccca gaacatgatt
     7321 ttgatatggc ttcagttgct aaaaaaatcc aagaaggccg cgatcgcggg aagaaacatt
     7381 gcttgatcat cttggctgaa ggtgtaatgg gcggaaatga attcgctgag aaactttcag
     7441 aatttggtga ctatcataca cgtgtatcta tcttgggaca cgttgtccgt ggtggagcac
     7501 caagcgcacg cgaccgcgtg ttggcaagta aattcggtgc ttacgctgtc gaattgcttc
     7561 aagaaggaaa aggtggatta tgtgtcggta tccatgacaa cgaaatcgtt gcgtctgaca
     7621 tcattgatac acttgaaaac aacaaacata aaccagactt atcactatat gatctaaata
     7681 attcactttc attttaagag tgaaagaaac caagctattt aagctgtgta cagctttaaa
     7741 taaacaaaat tgaatgggag agttttttaa atgaaaaaaa ccaaaatcgt tagtacatta
     7801 ggaccagcaa gtaactcagt agaaattatt tctcaattga ttgaatctgg ggcaaatgtc
     7861 ttccgcttta atttttctca tggtgaccat gaagaacaat tatcacgtat gactatggtc
     7921 cgtgaagcag ttaaaaagac aggaaaagat gtcggtattc ttttagatac aaaaggagct
     7981 gaaatccgta caactgttca aggcacaaca gaagctgatt ttggtcgtgc aggatacatc
     8041 aaatttgaag taggagatca aacacgcatc tctatggatc ctgaacatgt aggttcaaaa
     8101 gaaaaaatcg cggttactta cccaggatta tttgacgatg ttcacgttgg cggacacatt
     8161 ttatttgatg atggtttgat cgatatgcaa atcattgaaa aagacgaagc aaaccgtgaa
     8221 ttagtcgtag aagttaaaaa tgcaggtatg ctaggatcac gtaaaggggt aaatgcacca
     8281 ggcgtttcta ttagtttgcc aggtattact ccaaaagatg ctgacgatat tcgttttggt
     8341 ttagacaacg atatcgactt tattgctgca agttttgtac gtaaagcaca agacgttcta
     8401 gatatccgcg aaatcttaga agaaaaagac atgactcatg ttcaaatctt ccctaaaatc
     8461 gaatctcaag aaggtatcga caacattgac gaaatcatca aagtttctga cggtgttatg
     8521 attgcccgcg gagatatggg tgttgaaatc ccagctgaac tagtaccaat ggttcaaaaa
     8581 cgcattatca aaaaatgtaa tgctgctggt atcccagtta tcactgctac acaaatgtta
     8641 gaatcaatgc aagaaaaccc acgtccaaca cgtgcggaag cttctgacgt tgctaacgct
     8701 gtatttgatg gcacagatgc aacaatgcta tctggtgaat ctgcaaacgg ggactaccca
     8761 gttgaagctg tttcaacaat ggcacgtatc gaccaagaag ctgaaaatgc attatcagaa
     8821 ttaggtacat tccaattgaa cgaattcgat aaaacagatg taactgaaac aatcggttta
     8881 tctgtagctc gtgctgcaaa aaatctaggc gtgaaaacaa tcgttgctgc aactgaatca
     8941 ggatacactg caaaaatgat ttcaaaatat cgtccagatg cagatatctt ggctgttact
     9001 tttgacgaac gcacaaaacg tggtttgatg ttaaactggg gcgtttaccc aacagttgct
     9061 gaaaaaccat caacaactga tgaaatgttt gaactagcag ctaaaaaagc tgttgaatta
     9121 ggtttcgcat cagaaggcga tttaatcttg atcactgctg gtgtaccagt tggtgaacgc
     9181 ggaacaacta acgtgatgaa aattcaaatg attggttcta aattattaga agctcaaggc
     9241 gttggacaaa aatctgtcgt tgcaaatgca gttattgctt catctgctga agaagctgta
     9301 aaaaaagcaa aagacggaat ggttctagtt gtaccatcaa ctgacaaaga atatatgcct
     9361 gcaatcgaaa aagcagctgc tgttatcgtt gaagaaggcg gattgacttc tcacgctgca
     9421 gttgtaggaa ttgcacaaga tattcctgta atcgttggtg ctaaaggcgc aacaacaaca
     9481 attacagaag gtgaattgat taccgttgat tcacgtcgcg gcatcgttta ccgtggcgaa
     9541 acaacagcta tctaatttag cttaataaaa aataaaaaca gctcttcttt tacgaagagc
     9601 tgtttttttg tgctgtaacg aattcatgga taacttggac tacttgttct tgaagaggcg
     9661 tgacgatctg ctgtgttcgt tttgccaatg aaatcaagaa gcgaggctga acttcatctg
     9721 aaagagggag gcaaactaaa tgatcttcag gatggacagc aatctctgcc aaaaaaccga
     9781 tacccacatt ttctcggacc atactcttta aaatcgtcaa gtcattgctt tgataaacaa
     9841 cttttggatg agtatgtgtt tgtttagaaa gcttagtgaa tgctgcgggg tggacataat
     9901 gttcgttcaa tagtacaaac tgttcatctg ctatatcttt gaaagagaca gtatcctgct
     9961 tggaaagagg atgtgtaggt ggcacgacga tcatgaaacg tttgtccaaa agtaactctg
    10021 tttctaaata ttgatccacg attggctgtg ttgagcctaa tacagctaaa tcgatcctcc
    10081 cttgtcggat catcccgtat aagtcccgag agcctccatc ggtaatctca atttgattca
    10141 tcaattcatg cttgataaag taacttgaca gtttaggaaa gtattcattg ccgataatag
    10201 gaggcatgcc aaaacgaagc tttttctttt gcaactgagt gatttcagct cttgcagtag
    10261 aaatctcttg gagaatggtt tgtgcatgga gaaacagctg ctttccagaa ggcgtgacga
    10321 ttaattcgtg atgcgatcga tctcttgtaa taagtacggt gtcaaactct ttttcaagac
    10381 gttgtaatgc atatgtaata gtaggttggc tcacacggaa ataattagcc actttcgtaa
    10441 aatttttttg tttgacgatt tggacatagt attcgatatc tttcgtgttc atgaagatca
    10501 tccgctcctt taatggaata atgattattg taaatgattc tgaggatgat agcaaacaga
    10561 ggctttctaa taaagatttt tctgtttttt tatgaactgc aaggtgtcaa gaaaatttca
    10621 ttgacaacat gctttttgcc ctgtataatt gcttttgttg cttaggagtg atgaaaaatg
    10681 aaatggtctt tattggaatt gagaaaatac caggagacac cgttaacttt tcatgagacg
    10741 ctcgacttga aagccgctct catgaagcga gacaatttaa tacttgatgt tgcgccagtg
    10801 gaagtcgaag gtttggtctc agttgataag aaaggctata tcgttcatta cactgtgcag
    10861 acgactttga cagttccttc aacacgttca ctagaacctg ttgaactgcc catggatttt
    10921 tctgtagacg aagtattcat gacagaagaa caattccaaa gtagagatga acaaatgtct
    10981 gcggatgaga ttttgctaat tgaaggtcca ttattgaatt tagcagagtc gattgaagac
    11041 aacatcttat tggcgattcc aatgcgggta ttgacaaaag aagaggaagc agcaacagaa
    11101 cttccaaaag gcaatgattg ggaagttgtt tccgaagaag aataccagcg cagaaaaatg
    11161 gaagaagccg agaataaaat tgatcctcgt cttgcaaaac tatctgaact ttttgattct
    11221 aaatcaaaag acgacgataa caagtaagat tggaatatat tgtgtctaat ttagatacaa
    11281 gactttacaa ggaggtgtag caaaatggca gtaccagcta gaagaacatc aaaagctaaa
    11341 aaagcaaaac gtcgtacaca ctacaaattg acaatcaaag ggttaaacga atgtccaaac
    11401 tgtggtgaaa tgaaaaaaag ccatcacgta tgtgcaaatt gtggttacta tgatggtaag
    11461 gacgtaatgt caaaagaagc ttaatacatt ttaattagaa aatgtaaaac ctcaaggcta
    11521 accatggagc cttgaggttt atttttttgt agtcggtaaa taaaaaatat gttgttcttt
    11581 agtttgcaat gtagccgtgt taagctataa tgtaagagat tagggagaaa tcccgtatgt
    11641 aggaggagtt catatgtcaa aacaacaaat cggcgttgtc ggtatggccg ttatgggtaa
    11701 aaatctagcc ttgaacatag aaagccgcgg ctattcagtg gctttattca atcgtacagg
    11761 ggctaaaaca accgctgtag tagaagaaca cccagataaa aatttcaaag caacatatac
    11821 aatcgaagaa tttgtagaat ctatcgagaa accacgtcgg atcttgctaa tggtcaaagc
    11881 aggaccggca acagatgcaa cgatccaaga gttgcttcct caccttgata aaggcgatat
    11941 tttgattgac ggaggaaata cattctttaa agatacgatg cgtcgtaatg aagagttagc
    12001 caactctggc atcaacttta tcggtacagg cgtttccggt ggtgaagaag gagctttaaa
    12061 aggaccttca atcatgcctg gtggacaaaa agaagcctat gcgctagttg caccaatctt
    12121 agagcaaatt tctgcaaaag cagaagacga agcaccatgt gtcacatata ttggcccaaa
    12181 tggagccgga cattatgtga aaatggtaca caacggtatt gagtatggag atatgcaact
    12241 gattgctgaa tcttatgact taatgaagaa tattttaaac ctttcagtag aagaaatggc
    12301 tgatatcttt aaagaatgga atcaaggcga attagacagc tatttgatag aaatcactgc
    12361 tgacattctg acacgtaaag acgatgaagg aacaggtaaa ccagtagtag atgtgatcct
    12421 tgatgcagct gggaataaag gaacaggtaa atggacaagc caaagcgcgc ttgacttagg
    12481 tgtgccactt ccattgatca ctgaatctgt atttgcccgc tatatttctg cttataaaga
    12541 agaacgcgta caagcaagca agattttatc tcgtacgaac gattttgagt tcacgggaga
    12601 caaaaaagaa ttggtcgaaa aaatccgaga agcgttgtat ttcagtaaaa tcatgagtta
    12661 tgcccaagga tttgctcaat tacgtgtcgc ttcaaaagaa tttgattggg acttgccatt
    12721 tggtgaaatc gcaaaaatct ggcgcgctgg atgtatcatc cgagctcgat tcttgcaaaa
    12781 aattacggat gcctatgaca aaaatcctga gatcgaaaat ttattgctag atgattactt
    12841 cgttgaaatc acgaaaaaat atcagcaatc agtcagagac gtcgttgcct tggctgtcca
    12901 agctggcgta cctgtaccga ctttctcatc agccatcgct tacttcgatt catacagagc
    12961 agaacgtctg cctgcaaata taatccaagc ccaacgcgat tactttggcg cacataccta
    13021 tgaacgtgtc gacaaagaag gaatcttcca ttactcatgg taccatgaag aataatagtc
    13081 tttcggctgt taggaaatga gaaaataaaa agaggacgaa agagctgaag tcgattgtta
    13141 tgagtagact tcagctcttt tttaaaattg ctatccatga taaaatgact atagattttt
    13201 aagcatatta tcggtataat gatagagttt ttaaaaagcc agtagaatag aaaggataaa
    13261 ataataaatg agcaatattc taatcattga agatgaaaag aacttggcac gatttgtcga
    13321 gctagagttg aagcatgaag ggtataacgc agaagtccac tacaacggcc gaactggttt
    13381 agatgcggca ttaaataatg agtgggatgc catattgttg gatctgatgc tcccagaatt
    13441 gaacggttta gaagtctgca gacgtgtacg ccaagtcaaa aatacaccaa tcattatgat
    13501 gactgcaaga gattctgtga tcgatcgtgt atctggactt gaccacggag cagatgacta
    13561 catcgtcaaa ccatttgcta tcgaagaatt attagcacgt cttcgtgcat tgttgcgtcg
    13621 aatcgatatt gaaggcgata aaaatgtggc taaacaaact actattactt atcgcgattt
    13681 gactatcgaa aaagaaaacc gtgtcgtacg tcgcggaaat gaagtaattg aactaacaaa
    13741 aagagaatac gagttgctgc ttactttaat ggaaaacgtg aacgtggtat tagcacgtga
    13801 cgttttacta aataaagtgt ggggatacga aacagaagtt gaaacaaacg tagtggacgt
    13861 atatattcgt tatcttcgca acaaaattga cgttcccggg gaggaaagct atatccaaac
    13921 tgtacgcgga acaggttatg tgatgcgctc gtgataaagc cgaaaaaatc ggaaaaaaga
    13981 gaactgaaag ggccctcctt gacaatcaag tgggcctttg cgagttcttt cttcatattc
    14041 gttgtgttta caattttcgc tgtcattacc tataaatcat ctatcaatct gattgtggca
    14101 aaagaaagaa acaatgtaga gcgtacgatt tcagaagtag cttctcgttt ggctaactcg
    14161 gatgaagaac tgacattagt aaatacgtat cgtaatctga cgaaagcgac aaatgacgga
    14221 gatgaggtcg tagatcatac ttcactggtc gaaggaaact taatgaagat cgattccttt
    14281 atagctgagc tagggcagcc atcaatggat ctttatgttt atgatttgga cgagaacttg
    14341 atttttaaaa cccatgaaaa cagtcgaaac ctgattcaga ctacacgtaa agttccaacg
    14401 atcgttacac tagaaggaaa aactggcttt ttatctatcc agcctatata ttctaaaggt
    14461 acaagagaaa agatcggcta tgcgcaaagc ttctatgaat tatcttcttt ctatgatatt
    14521 cgaaataagc tattattgac tcttattgtt ttagaagttg tttctttgat cttaagtagt
    14581 attttaggtt tctttttatc ttcttatttc ttaaaaccac tcaaggtact cagagataca
    14641 atggatacta tacgtaaaga tccgcaatca gatatccaca tgcctgaaat cgacacaaat
    14701 gatgaattgg cagatttagc tgaaatattc aatgagatgc ttgaccgtat gcgtttgtat
    14761 attgaacaac aagagcaatt cgtggaagac gtatctcatg agttaaggac acctgttgca
    14821 attattgaag ggcatcttag tctgctaaat cgttggggga aagatgatcc tgagatatta
    14881 gaagaatcat taagcgccag catgcaagaa attagccgta tgaaaagctt agttcaagag
    14941 atgctagact tatcaagagc agaacaagta gatgttcatt atgcgaatga aacaactaaa
    15001 ggaaaagaag tcacctatca agtttataat aatttcaaga tattatatcc agactatgtg
    15061 atcactttag atgatgattt gacaaaagaa gtgaccctgc aaatttatcg taatcacttt
    15121 gaacaattaa tcattatcat attggataac gcagtaaagt attcaactac acgaaaagaa
    15181 gtccatatct ctatttcttc cactctgaat gaatttgaga tcgcgattca ggactttggc
    15241 gaaggaatac ccaaagaaga ccttaataag atcttcaatc gtttttatcg agtggataaa
    15301 gcaagagcta gaacgaaggg tggaaatggt ctggggttgt ctatcgctaa acaactggtt
    15361 gaaaactata aaggacgtat cttagcagaa agtgtagtag ggcaagggac aatttttaga
    15421 atctttattc ctatcgcaaa ggcagaagac aagtaaagca tgtgctttac ttgttttttt
    15481 atatatccaa caaagaataa ggtacaaaat acaagcaagt tagaaattca ggaatgtcat
    15541 tataaaaaaa actagcgaac aattaaatag aataacatcc gttaattaaa cgtaagtagc
    15601 ttttagatga acgaaaaaac aatgaaatgc tgtaatattc gtgtttaaaa ataataaata
    15661 aaaataggga attttctatt gcaattttat aaagagattg ctatattatt atttgttcta
    15721 aatgattcct ttaaggtgtc actaaaaatt ataaaaaata gggttgacgc acttttgatg
    15781 tcatgttata ataacgaagt cgtttggatg aggtagctaa taaagcttga ttttatcgaa
    15841 aaaaacttcg aaaaagttgt tgacaaataa caataaagat gatatgataa tcaagtcgct
    15901 gctaatgagt gacaaagacg tttagaaaaa ataaacaaag aagttgttga cattctcttc
    15961 tgaatctgat aagattaaag agttgttgaa acaaaaaga
//
LOCUS       DAZUKD010000058        15950 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000058 DAZUKD010000000
VERSION     DAZUKD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.059"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8N9_001656"
     CDS             226..894
                     /locus_tag="ACG8N9_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4897234.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="ACG8N9_001657"
     CDS             complement(1094..1324)
                     /locus_tag="ACG8N9_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897235.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1321..1479)
                     /locus_tag="ACG8N9_001658"
     CDS             complement(1321..1479)
                     /locus_tag="ACG8N9_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897236.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKMKKKHA
                     NSLFLNNK"
     gene            complement(1451..2833)
                     /locus_tag="ACG8N9_001659"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8N9_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4897237.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8N9_001660"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8N9_001660"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4897238.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8N9_001661"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8N9_001661"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4897239.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8N9_001662"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8N9_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4897240.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="ACG8N9_001663"
     CDS             6859..7764
                     /locus_tag="ACG8N9_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4897241.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8N9_001664"
     CDS             7855..8532
                     /locus_tag="ACG8N9_001664"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4897242.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8N9_001665"
     CDS             8529..8699
                     /locus_tag="ACG8N9_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897243.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8N9_001666"
     CDS             8813..9709
                     /locus_tag="ACG8N9_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4897244.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8N9_001667"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8N9_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4897245.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8N9_001668"
     CDS             11372..12118
                     /locus_tag="ACG8N9_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4897246.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8N9_001669"
     CDS             12115..12954
                     /locus_tag="ACG8N9_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4897247.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8N9_001670"
     CDS             12955..13905
                     /locus_tag="ACG8N9_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4897248.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8N9_001671"
     CDS             14046..14798
                     /locus_tag="ACG8N9_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897249.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15950
                     /gene="queG"
                     /locus_tag="ACG8N9_001672"
     CDS             14817..>15950
                     /gene="queG"
                     /locus_tag="ACG8N9_001672"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008379556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4897250.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLL"
BASE COUNT         5169 a         2994 c         3215 g         4572 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg
//
LOCUS       DAZUKD010000059        15861 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000059 DAZUKD010000000
VERSION     DAZUKD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.060"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACG8N9_001673"
     CDS             68..751
                     /locus_tag="ACG8N9_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4897251.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACG8N9_001674"
     CDS             753..1589
                     /locus_tag="ACG8N9_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4897252.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACG8N9_001675"
     CDS             1600..3354
                     /locus_tag="ACG8N9_001675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897253.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACG8N9_001676"
     CDS             complement(3630..3971)
                     /locus_tag="ACG8N9_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4897254.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACG8N9_001677"
     CDS             complement(4039..6210)
                     /locus_tag="ACG8N9_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4897255.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACG8N9_001678"
     CDS             6421..7278
                     /locus_tag="ACG8N9_001678"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4897256.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACG8N9_001679"
     CDS             complement(7349..8206)
                     /locus_tag="ACG8N9_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4897257.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACG8N9_001680"
     CDS             complement(8191..10893)
                     /locus_tag="ACG8N9_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4897258.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACG8N9_001681"
     CDS             complement(10906..12195)
                     /locus_tag="ACG8N9_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4897259.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACG8N9_001682"
     CDS             12345..13391
                     /locus_tag="ACG8N9_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4897260.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACG8N9_001683"
     CDS             13393..15546
                     /locus_tag="ACG8N9_001683"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4897261.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4507 a         3308 c         2927 g         5119 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgag tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccatttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat t
//
LOCUS       DAZUKD010000060        15799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000060 DAZUKD010000000
VERSION     DAZUKD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.061"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..779
                     /locus_tag="ACG8N9_001684"
     CDS             27..779
                     /locus_tag="ACG8N9_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4897262.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            776..1690
                     /gene="pfkB"
                     /locus_tag="ACG8N9_001685"
     CDS             776..1690
                     /gene="pfkB"
                     /locus_tag="ACG8N9_001685"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4897263.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1708..3639
                     /locus_tag="ACG8N9_001686"
     CDS             1708..3639
                     /locus_tag="ACG8N9_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4897264.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3750..5309)
                     /locus_tag="ACG8N9_001687"
     CDS             complement(3750..5309)
                     /locus_tag="ACG8N9_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4897265.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5464..7698
                     /gene="pcrA"
                     /locus_tag="ACG8N9_001688"
     CDS             5464..7698
                     /gene="pcrA"
                     /locus_tag="ACG8N9_001688"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4897266.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7715..9751
                     /gene="ligA"
                     /locus_tag="ACG8N9_001689"
     CDS             7715..9751
                     /gene="ligA"
                     /locus_tag="ACG8N9_001689"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4897267.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9857..10162
                     /gene="gatC"
                     /locus_tag="ACG8N9_001690"
     CDS             9857..10162
                     /gene="gatC"
                     /locus_tag="ACG8N9_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4897268.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10162..11634
                     /gene="gatA"
                     /locus_tag="ACG8N9_001691"
     CDS             10162..11634
                     /gene="gatA"
                     /locus_tag="ACG8N9_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4897269.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11631..13061
                     /gene="gatB"
                     /locus_tag="ACG8N9_001692"
     CDS             11631..13061
                     /gene="gatB"
                     /locus_tag="ACG8N9_001692"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4897270.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13071..14126
                     /locus_tag="ACG8N9_001693"
     CDS             13071..14126
                     /locus_tag="ACG8N9_001693"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4897271.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14237..15610
                     /gene="rlmD"
                     /locus_tag="ACG8N9_001694"
     CDS             14237..15610
                     /gene="rlmD"
                     /locus_tag="ACG8N9_001694"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4897272.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15613..15741
                     /locus_tag="ACG8N9_001695"
     CDS             15613..15741
                     /locus_tag="ACG8N9_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4897273.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5372 a         2897 c         3469 g         4061 t
ORIGIN      
        1 ttatttgtat tgaggtgatc gtgaaaatgc ttacagagga acgtagacaa aaaatcctag
       61 acttgcttga acagcagaaa atcgtgaaat cacaagatct tgtaaacctt tttaatgctt
      121 ctgaatcaac gataagacgt gatttacaag agctggagaa tagtgggttt ctagaaagag
      181 ttcatggcgg agctaagaaa gagcaattat tgaattttga gcagaatatg agtgaaaaat
      241 cactcaaaaa cgttcacgaa aaacagcaaa tcgccaaatc ggcagctgaa accattaatg
      301 acgaagatgt catttattta gacgctggct ctactacgtt agaaatgatt ccttttctgc
      361 aaggaaaaca gatcacagta gtgacaaatt ccgttcaaca tgcggcaaat ttagttgatt
      421 tggagcttgc taccattatt ttaggtggaa cgataaagct ggcaactaat gctgttttag
      481 gatctaatgc gatagaacaa ctaaaaaatt ttcattttaa taaagcattt atgggaatga
      541 acggtgccca tcttgaacaa ggattcacga ctccggaccc agaagaggca gctgttaaac
      601 gtttagccat gaaaaattcc gaagagacat ttgtattgat tgatcatacc aaattcaacc
      661 agttgtcctt cacctctgtt gcacctttgg aagcagccac gatcataacc gagcgttgtc
      721 cattagaatt cgttaatgaa ttccgtgaaa agacaacaat taaggaggca atacaatgat
      781 ttacacagta acacttaatc catcaattga ttatatcgta cgcgtagatg ggttaaaact
      841 aggcgatttg aaccgaatga aggaagactt caagttacct ggaggaaaag ggatcaatgt
      901 ttcccgtatt ttgaaacgga tcgattctca atctacagca ctagggtttt taggagggtt
      961 cactggcgaa tttatttcga agtggcttga aaaagaacaa atccagtctt tgttcacaac
     1021 cgtaaaagaa gatacaagaa tcaatatcaa actaaaatca gatactgaaa ctgaaattaa
     1081 cggtttgggc ccatctattt cagatgaaga aatcgatgag ttgaagcaag cattaggaac
     1141 agtaggacaa ggagatatcg tcgtattgtc aggaagcgcg ccggctagcc tgcgaaaagg
     1201 attttatcaa gaactgattg atatcattcg agaaaaaggt gcagatttcg tgattgatac
     1261 aacaggtgat gacctaaaaa atgcacttgt taagaaacca ttgttgatca aaccaaacaa
     1321 tcacgaatta gctgagcttt acaatacaac cttccaatct gtagaagaaa tcattccgta
     1381 tggtaaaaaa ttgctagctg aaggggcaca gaatgtgctg atctcaatgg ctggagacgg
     1441 tgctttgctc ttcacggaac aaggaactta taaaacgaat gtattagttc gcccattgaa
     1501 aaattcagta ggtgcagggg attcaatgat tgccggcttt attggggctt acagccgtac
     1561 gaaagatcca atcgaagcct ttaaatgggg agttgcttgc ggaagcgcaa cagctttttc
     1621 tgatgacttg gcagctcgcg aattgattga tgagttgctg ccagaagttg aaatcacaaa
     1681 aattgattag tttcaaggag gataatcatg aatatcaagg atttaatggt caaagaagcg
     1741 atgatcatgg atttgcaagc gactgataaa aaaggcgcaa tcgatgaaat ggtccaaaaa
     1801 atgtttgatg ccggccggat ttccgatatc gaaacatata aagaaggaat tctagcccgc
     1861 gaagcacaaa cttctacagg tcttggtgat ggaatcgcca tgcctcacgc gaaaaacagt
     1921 gcagttaaag aagcaacagt tttattcgca aaaagccaaa aaggagtaga ttatgaagcc
     1981 ttagacggac agccaactta tctgtttttc atgatcgcag ctccagaagg cgcaaacgac
     2041 acacatttgc aagcattagc agcactatct cgattgctga ttgatgcaga tttcgtagga
     2101 aaattaaaag aagcaaatac accagaagaa gtccaagact tgttccaaac agctgaagag
     2161 caaaaagaag cagaagaaaa agcagaacaa gaaacacaag ctgcagaagc ttctgctcat
     2221 ccagaaagaa aattcgttgt tgccgttaca gcttgtccaa cagggatcgc tcacacttac
     2281 atggcagaag atgctctgaa gaaaaaagcg aaagaaatgg gtgtggagat caaagtcgaa
     2341 acgaacggtt cagaaggaat caaaaaccga ttgactgctg aagacatcgc ccgtgcagaa
     2401 ggtgtaatcg ttgcagcaga caaaaaagta gagatgaatc gctttgacgg aaaagaactg
     2461 atcaatcgtc ctgtcagtga tggtatccgt aaaccagaag agctgatcaa tctagccctt
     2521 agtggtactg caccagtatt ccatggtgat ggaaaagaaa gcagttcaga agaaggaaat
     2581 gcggatggta ctatcggaca aagaatttac aaagatttga tgaacggcgt atcacacatg
     2641 ttacctttcg ttattggtgg aggaatcgct attgcgcttt ctttcatgat cgaccaattt
     2701 attggcgtgc cacatgatca attatcaaat ctagggaatt acaatcaagc tgctagctgg
     2761 ttcaaccaaa tcggacaagc tgcatttggt ttcatgctgc cggtattagc aggatttatt
     2821 gcttcaagta tcggtgaccg ccctggtttg atcgttggat ttgctgctgg tgcattagct
     2881 aatacaggcg gtgcaggatt cttaggtgca ctgattggtg gattcttagc tggttatgtc
     2941 atcgtatttt tacgtaaatt attcaagaac ttaccaaaat cattggatgg aattaaaacg
     3001 atcctctttt atccagtgtt tggcttactg attactggtt tcttgatgtt gctggtcaat
     3061 gtccctatga aagcaatcaa tgatgcattg aacggtttct tgacaagtat gagtggttcg
     3121 aatgctgctt tacttggtgc attattagct ggaatgatgg cggcagattt aggcggtcca
     3181 atcaacaaag ccgcttatgt gtttggtaca gcaactttag ctacgactgt tgcaactggc
     3241 ggaagtgtcg tgatggcttc tgttatggct ggaggaatgg tacctccatt ggcaattttc
     3301 gttgctacac gtttgtttaa gaataaattc agcaaaacag atcaagatgc tggtttgacg
     3361 aacatcgtga tgggactatc gtttgtcacg gaaggatcga ttccatttgc agcagctgac
     3421 ccgattcgtg ccattccaag ttttattatt ggttctgctt taactggcgg attagtcgga
     3481 gctttcggga tcaaattact ggctcctcat ggcgggattt tcgttgtatt cttactgagt
     3541 cacccggtca tgtatttggt gtttattgct atcggagcag tcgtatctgg tgtgatttat
     3601 ggtttattga gaaaagctcc tgcagcagca gaagcataga aataaaaaag aagaggctgg
     3661 tttttcccag tctcttcttt ttttatagaa tcatatagaa tcaagtagtg tagatgaata
     3721 gttgttgtaa aatagttttt ccatgttcgt taaaatgatt gcttcaaatt tgtgatagaa
     3781 gatttagcct cgatttcctg cttgattctt ggcaagtttg cttcatcagt caagataacc
     3841 aaaatatctc ctacattcat gatcgtatca ccgtgagtca agatttcctg atttcctctg
     3901 cgtatagaga ttagcagcat atttttaggc caattgaagt ctcgtaccat taatccgtct
     3961 aaagaactct ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt tagtgaggcg
     4021 gtctgagtat ttccaagtag acgttccaat aatgattcgt aaattggatt accgccgaga
     4081 aggtcattga cgatataagc aacaagagca gccaatccta aaggcatcag atgattaaga
     4141 gagccaacca tttctgtcac taaaataatt gctgtcagcg gtgcttttcc aatcgctgtg
     4201 aaataaccag ccatggcaat aacgacaaaa tccttgatgt agattggatc catcccgaaa
     4261 tcaaccataa tgtttccata aaagctaccg attaaagccc ccaatgtcaa tatcggaagg
     4321 aaaatccccc ctggaagacc accaccgtaa gaaatcatgg aaaaaacgaa tcgaagaaca
     4381 aacaaaccga taatcataat gattggcaag gaagcatgtc caaaagctaa aacgatttga
     4441 ttgcctccgc ctagagtact aggccataaa taaccaattg gcaaaatcag caagaaagga
     4501 acgaacccgt gaaaataagc tggtaaaggc aatcggttgt aaaattttgg caaagccagc
     4561 aagaccttct gataaataaa acctaagatg cctagaagta ctcctaagat cactaaggaa
     4621 ccataatatt tcaacggcaa agaaggaagg tcacctagat ataaaacagg tctcaagccg
     4681 aaaaagtaaa gagagacgaa gttagcagtg attgctgaag caaatgatgt cagccaaaca
     4741 agtggagaaa aggagtgatg gatttcttct aaaacaaaga gtaatcctgc gataggagca
     4801 ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt ttcttctgaa
     4861 gatggactcc gaaacaattg tccagctcct tgtccaatgg atgcgccaag ctgtatagaa
     4921 ggtccttctc tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc aatgaacttt
     4981 ttccataaaa cagagaacca gttcattgat aattccccgc gaagctgtcc ctctacttgt
     5041 ggaatcccgc ttcccttgat atccggttca tttttgacca gataacccac gaaaaatgct
     5101 gcaaggatgc agcaaatagc taggccaatt aagtaaatcg gctgttcccg actcatttca
     5161 tagatacttc gaactaaatc gcttagcttt tcgatagata atcgaaacag gctaacgacg
     5221 attcctgtca gcagtccaac aaatattcct tttagaataa aatatatctg agtcttatct
     5281 aatttcttga tttttccttt tttttccatt caaacgcctc gctttctttt aatttagcgc
     5341 gaaatcaacg aattgacaag gaaaaatcca taaaaagaaa caaatgattt tgaactagct
     5401 agtttaatga aagccaagaa aatgctataa ttaacaagat tgataaaaga aaggatgttg
     5461 ttaatgtcat ccaaagctga attattaaac gggatgaacc cccgtcagaa agaagctgtt
     5521 cttcacactg acggaccatt actgctaatg gcaggtgcag gaagtggaaa gacgagagtg
     5581 ctgacacacc gcattgccta tttgattgag gaaaaagaag tcaatccttg gaacatccta
     5641 gcgattactt ttaccaataa ggctgcaaaa gaaatgaaag aacgtgtcaa tgcgattctc
     5701 gcatctggag gcgaagatgt ctgggtatcg acttttcact cgatgtgtgt gcgtatttta
     5761 agaagagatg ttgattttat cgggtataac cggaacttta caatcatcga ttcttctgaa
     5821 cagctgacct tgatgaaacg gatactaaaa gaactgaaca ttgatccgaa aaaatatgat
     5881 cctcgaagta tattaggaac aatctctcaa gcaaaaaaca gcctgcagac ccctcaggat
     5941 tttgcaaaaa tgcaaggcag ttattacgaa gagattgcgg caaaatgtta tgctgcttac
     6001 caaaaagagc ttcaatataa tcaatgtatg gactttgacg acctgatcat gaatacgatt
     6061 cgattatttg aagaacatcc tgattcactc acatattatc aaaacaaatt ccattatatt
     6121 catgtagatg agtaccaaga tacaaatcac gcacagtata cgctagtcaa tttattggca
     6181 ggtagattta ggaacctgtg cgtcgtggga gatgcagatc aaagtattta cggctggcgc
     6241 ggtgcagata tgcagaacat cttggatttt gaaaaagatt atccagatgc tgcagtaatt
     6301 ttgctagaac aaaactatcg ctccacgaaa aacatcctta gcgcagcaaa ccaagtcatt
     6361 gaaaacaata gcaatcgaaa accaaaaaac ttgtggacag aaaacaaaga aggaaacaag
     6421 atcacttact accgtgcaga taatgaacga gacgaaaccc gttttatcgt agatcggatg
     6481 caagaagaga ttcgaagcaa tcatcggaat tacggcgact ttgccatctt gtaccggacg
     6541 aacgcacaat ctcgtgtaat ggaagaaact ctgttgaaag caaatattcc ttataaaatg
     6601 gttggagggc acaagttcta cgatcgtaaa gaaatcaaag atattttagc gtatttgaat
     6661 gtgctggcaa atcctcaaga ttcaatcagt tttgagcgta tcgttaattc gcccaaacga
     6721 ggcatcggac caggatctat cgaaaaacta cgcagttttg cttctcttca tgaatggcca
     6781 ctgcttgaag cagcgcaaaa tgtggatctg gcaaatatcg gtggaaaagc aggacagcaa
     6841 ttaggtgcat ttggagagat gatccaagaa gtcactcaga tgatcccata cttgacagtg
     6901 acagaattga cgaaagaggt tttggatcgt agcggatatc tagaagactt aaaaatccaa
     6961 aatacattag aagcacaagc acgcatcgaa aacttggagg aatttttaac ggtcacacaa
     7021 gaattcgaca aacaattcga acagcaaaat gaagaagacg ctgatgcacc agaagaaaaa
     7081 ctaacggtct tcttaaatga tctggcactt gtttccgata ttgacaattt agaagaagat
     7141 gcttcccaag tgacattgat gactttgcat gcagccaaag gattagaatt cccagttgta
     7201 ttcctgattg gtctagaaga gggggttttt cctttatctc gagcattgat ggaagaaagc
     7261 gaactggaag aagaacgccg tctcgcatat gtggggatca cgcgagcaga agaagcgttg
     7321 tatttaacaa atgctttctc cagaacatta tatggacgga cgcaatataa ccgaccttct
     7381 cgttttgtag aagaaatcga tcaggagtta ctggaaatcg aaggcatgcg tcctacgcct
     7441 aaaaaaacac ctgtattcgc taaaaaaaca gcatatagct ataaacagcc agagacagcg
     7501 gttgttccct ctaaaagtgc tactggaggg gaaaataaca gctggaaacc tggagataaa
     7561 gtcaaacaca aaaaatgggg tctaggaaca gtcgtccgag taagcgggac gtcgaaggat
     7621 ctagaactag atgtggcttt cccaagtcaa ggagtaaaac gtcttttggc tgcctttgcg
     7681 ccaattgaaa aagcttagta gtggagggta aatgatggaa catgaaatga cgattgacga
     7741 agctgctaaa cgagcagaag aacttcgtac aagactgaac caatggtctc gcgaatacta
     7801 cgtagaagat aaaccaacag tggaagacta tgtttatgat aaagaatacg cagaattagt
     7861 agcaattgag gaacaatatc ctgatttgat cacgtctgat tctcccactc aacgagtagg
     7921 cggaaaagta ttagaaggtt ttgaaaaagt cactcatgat attccgcttt acagtttgaa
     7981 cgacgttttc agcaaagaag agctgatagc ttttgatcaa cgtgtacaaa aagcagtggg
     8041 acgtgtagtt gactattgct gtgaactaaa aatagatgga ctttccgttt ctttgcgtta
     8101 tgaagatgga aatttcgtga gaggtgcgac tcgcggggat ggaactgtag gagaaaacat
     8161 cactgaaaac ctaaaaactg tccggtcggt cccaatcaaa ttaaaagaac caatgaacat
     8221 cgaagtaaga ggcgaatgtt tcatgccaaa acggtcattt gtccaattga accaagacag
     8281 agaagcagag ggaaaagata tctttgcgaa tcctcggaac gccgcagctg gttctttgcg
     8341 tcagttagat tctaaaatca cagcgaaaag aaatctggac acttttttat acacagtagc
     8401 tgatttcggt cctatgcaag caaaaaccca gtatgatgcg ctagaagagc tagaaaaaat
     8461 tggattccac acgaatcggg aaaaacgtct atgtcattcg attgatgaag tgtggtcata
     8521 cattgaagaa tatcatgaca aacgtgtgga tttgccctat gaaatcgatg gaatcgtcat
     8581 caaagtgaat gaattctcgc tacaagatca acttggtttt actgtcaaag caccgagatg
     8641 ggcagcagct tacaagtttc cgccagaaga agttgaaacg ctaatcgaaa acatcgaatg
     8701 gacagtcggt cgaacagggg tagtaacgcc aacagctatc atgacaccag tccgagtagc
     8761 aggaacgaca gtaagccgtg ccagcctgca taacggtgat tatattaagt tgaaggatat
     8821 ccgcttgaaa gatacagttc tgatttataa agcgggagac atcatcccag aagtttcgca
     8881 agtcgtgtta gataaacgac ctaaagacag cgaagaatac caattgccta cacattgtcc
     8941 agtttgtgga agtgaacttg tccatttgga tgaagaagta gccttacggt gcatcaatcc
     9001 taaatgccct gctcaaatga aagaaggttt gaaccacttt gtttcaagaa atgcgatgaa
     9061 tatcgatggg ttggggccgc gcgtactaga acagatgtat gataaaaaac tagtagcaga
     9121 tgttgctgat ctttacaaat tgacagaaga agagttattg acgttggaca aaataaaaga
     9181 aaaatcagca aataatattt tgacagcaat cgacaacagc aaagataatt cggtcgagcg
     9241 gttgattttc gggttaggaa tccgacatgt cggagcgaaa gcagcaaaaa ttttagcaga
     9301 acatttcggt gatctagaaa cattgagtaa aagtgactat gagtcgatta ttgcgcttga
     9361 tacgatcggt gatatcattg ccgacagtgt agtcacttat ttcagtaacg aagaagtcca
     9421 tgaattgatg aatgaattaa aacaagcagg cgtcaatttc gaatacaaag ggcttcgaaa
     9481 tgcccaattg caagaagtcg agtctccttt taaagaaaaa acagtagtcc ttactggtaa
     9541 actaacacga tttactcgtg aagaagcaaa agagacgatc gaaaatcttg gcgggaaagt
     9601 gactggtagc gtatcgaaaa aaacggatat cgtggtagca ggagaagatg ctggcagcaa
     9661 gctgaccaaa gcacaagaat taggaatcga agtttggaca gaagatcaaa tggcagatgc
     9721 attggctaaa agtcgctcag tagaagaatg aaaaatgagt tttttcagac aaatttcaat
     9781 aaacaatttc agacacggca ctcttgtgtt aaaatagaga gcaaagtttt taactcgttt
     9841 agaaagaagg ggtagtatgg cgattagtga agaacaagtg aaacacgttg ccaaattagc
     9901 aaaacttgcg ttcgcagatg aagaacttgc aagttttacc gaccaattag gtaaaatcat
     9961 cgatatggtt gaaatgctgg aagaagtaga tacagaaggt gttccattta cgtcgaatgt
    10021 ggttgaaaca gtaaacgtga tgcgacaaga tcgggcgcaa gctggatgga accgcgatga
    10081 attattgaaa aatgtgccgg aaacagaaga cggctttatc aaagtacccg caatcatcga
    10141 taatggggag gctggcgcat aatgacagaa ttacacaatc gttctctaga agaactgcat
    10201 catatgcttg tatcgaaaga aatcactgta caagatttga caaaagcaac atttgaacga
    10261 atcaaagaaa cagagcctga ggtcgaagca ttcattacat tgaacgaaga aaaagcattg
    10321 gaacaggcaa aagctttaga tgaaaaaggg attgatgaaa gcaacgtcct tgctggtatt
    10381 ccaattggta tcaaggataa tatcgtaaca aaagatatct tgacaaccgc tgcatctaag
    10441 atcctttaca attttgaacc aatttatgat gcaacggtca tggataaaat ctatcaagca
    10501 gatatgatcc ctgttggtaa gctaaacatg gatgaatttg ctatgggtgg aagtacagaa
    10561 acttcttatt tcaagaaaac aaaaaatgct tgggacccaa caaaagtacc tggtggctct
    10621 tctggtggtt ctgcagcagc agttgcagct ggacaaattc cagtttcttt aggtagtgat
    10681 actgggggaa gtatccgcca accagcagct tttaacggca tcgtagggat gaaacctact
    10741 tatggccgtg tctcacgttt tggtttgatt gcctttgctt catctcttga ccaaatcgga
    10801 ccaatgaccc gtacggtgaa agataatgca ttagcattga cagctatcag cggttatgac
    10861 gaaaaagacg gtacttcttc aggcgtttca gttcctgatt ttgcagaggg actgacaggc
    10921 gacatcaaag gaatgaagat tgctttacct aaagaatact taggcgaagg tgtggcccct
    10981 ggcgtaaaag aagctgtatt gaaagcagca gaaacattcc gttcattagg tgctgttgtg
    11041 gaagaagtca gcttgcctca ttcaaaatac ggtgtggctg tttattatat cattgcctca
    11101 tcagaagcaa gttccaactt gcaacgattc gatggtatcc gctacggtta tcgctcagaa
    11161 aatgtgaaat cattagatga tgtatatgtc aactcacgtt cagaaggatt cggtgaagaa
    11221 gtaaaacgcc gtatcatgtt aggtacattc tccttaagtg ctggttatta tgacgcttac
    11281 ttcaaaaaag cgggacaagt ccgtacattg atcaaacgag attttgaaaa agtctttgca
    11341 gattatgact tgatcatcgg gccatcttca ccaacagttg cattcggaat cggtgagaac
    11401 attaacgatc caatcacaat gtacatgagt gacatcttaa cgatccctgt caacttggct
    11461 ggacttccag gtatgtctgt accggcaggt ttatcagaag gattaccagt tggattgcag
    11521 atcatcggta attattttga tgaaaaaacg atgtatcaag cggcatatgc gtttgaacaa
    11581 gcaacagaat tccataaaca acaaccagct attttaggag ggaaagacga atgaattttg
    11641 aaacagtcat tggactagaa gtccacgtcg agttaaaaac caactcaaaa attttctctc
    11701 cagcgccagc tcattttggt gcagaaccaa acagcaatac aaacgtgatc gactggggtt
    11761 atccaggcgt gctgccagta atgaataaag ctgcgctgga attcgggatg aaagcagctt
    11821 tggcattaaa ctgtgaaatc tctaaagata ctcattttga ccggaaaaat tacttttacc
    11881 cagataatcc aaaagcatat caaatttccc aatttgatca accaatcggt catgacggat
    11941 ggatcgaaat cgaagtagaa ggtaaaaaga aaaaaatccg gatcgaacgt gtccatttag
    12001 aagaagatgc tggaaaaaac atgcatggta ttggcggtta ttcttacgtt gatttgaacc
    12061 gacaaggcac gcctttgatc gaaatcgttt ctgaagctga tatgcgttca ccagaagaag
    12121 catacgctta tctagaagca ttgcgatcaa ttatccaatt tacagaagtc agcgatgtga
    12181 aaatggaaga aggctctatg cgctgtgatg ccaatatttc tcttcgccct tatggacaag
    12241 aagagttcgg tacgaaagca gaattgaaaa acttgaactc tttatctttc gtgaaaaaag
    12301 gtttagcttt tgaagaaaaa cgtcaagcaa aagttcttct ttcaggagga gaaatccaac
    12361 aagaaacacg ccgttttgac gaaacaacga ataaaacagt tttgatgcgt gtcaaagaag
    12421 ggtctagtga ttatcgttac ttcccagagc ctgatatccc gaaattcgtg atcgatgacg
    12481 aatggattca aaaagtaaaa gctagtttgc ctgagatgcc agcttctcgt cgcgaacgct
    12541 atatccgtga gtttggactt ccagagtatg acgcaatggt attgacactt acaaaagaaa
    12601 tgtctgattt ttttgaagct gttttggaaa atggtgcaga tgccaagcaa gcatctaact
    12661 ggttgatggg tgaagtttct gcttacttga acagtgagaa actggaatta gctgagacaa
    12721 aattgacacc tgagaactta gcaggaatga ttcgtttgat cgccgatgga acgatcagct
    12781 caaaaatcgc caaaaaagtc ttccgtgaat tgatcacaaa tggcggagaa gcccaagaag
    12841 tagttgaaaa aaatggttgg gtgcaattat ctgatccgaa caaactactg ccaatcatta
    12901 atgaaatttt ggataacaac caacaatctg tcgatgattt caaaaatgga aaagatcgtg
    12961 cggttggctt cctagtcggt caaatcatga aagcaactaa aggacaagca aatcctggtg
    13021 tcgtgaataa gctacttaaa gatgaattag cgaaacgata gaggctgaaa atgaaaaaag
    13081 cacgcgtaat ttataacccc acctcgggaa aagaattatt gaaaaaaaat ttggcggata
    13141 tcctacaggc attagaagaa gcaggatttg aagccagcgc ctatgcaact acaccagaac
    13201 cagattcagc caagaatgaa gcatgtagag tcgcagaact aggttttgat ctagtagtgg
    13261 cagctggtgg cgatggcaca atcaacgaag tggttaatgg aatcgctccg cttgaacacc
    13321 gtcctaaaat ggccatcatc ccagctggta caacgaacga ctatgctcga gcattaaaaa
    13381 ttcctagaga taatattaaa gcagcagcag aagtgataaa aaaacaccaa accgtaaaaa
    13441 tggacatcgg cctttctgat aacagttact tcatcaatat cgctgcaggt ggctatttga
    13501 cagaattgac ttatgaagtt ccatcagaac taaagagtat ttttggttat cttgcttatc
    13561 ttgcgaaagg agcagagatg ctccctcgag taaagccaat caaaatgcgc ttgaagtatg
    13621 atgaagggga atatatcgga aatgcttcga tgtttttcct agggctgacg aactctgttg
    13681 gaggattcga acagatcgcg ccagatgcca aattggatga tgggaagttt tctttaatca
    13741 ttgtgaagac agcaaatatc tttgagatct tgcatattgc agcactcatg ctgaatggcg
    13801 gtcgacatgt cgatgatcca agaataatct ataccaaaac aagcagtttg ttcgttgaaa
    13861 cagaagatga tatcaatcga ccagtaatga tcaacttaga tggtgaatac ggcggggaag
    13921 caccgatgca tttccaaaat cttcatcaac acattgagtt ctttgccaat accgatcaga
    13981 ttcctgatga agcaatcaca ggtacagatg aagaagaatt agaggttgtc agcaaagaat
    14041 tcgtaaaaga agtcgaacgt ttaacggatg aagatatcga cggtgacgga aaaatagcag
    14101 acgaaacacc taatcaaaag aattaatcaa aagccggtgc tgaacgtagt aaggtcagtg
    14161 ccggttttag ttgtgtaatt agctgattta tagtagagtt cagtaggatt gaaaaatagg
    14221 cttaaaggag caaaatatga cagaacagca aatagtaaag aaaaacgaac gatatacagt
    14281 cgaaatcgtc gacttgagtt atgaaggaat gggggtagca aaaatcgatg gttatccatt
    14341 atttatcgaa aatgcactcc ctggagaaga agtagaaata ctagtagtaa aagcaggcaa
    14401 taagtttgga tatggaaaag tcgaaacatt tattacacaa tcacctgatc gtcagcctgt
    14461 agaaaacaat gcattacttc gcataggtat cgcaccttta gcccatttga agtatgaaca
    14521 acagcttgtc ttcaaacaaa aacaagtaga gcatgtaatg gcgaagattg ctaagatgcc
    14581 agaagttcct gtacttccaa cggtcggaat ggcagaacca ttcggctacc gtaataaagc
    14641 acagatccct gttcgccgta tcgatggaca gttagccacc ggtttttaca aaaagaatag
    14701 ccatgatcta gtccctatcg aagattttta tatccaagat cctgtcatcg atcaagcaat
    14761 caaagtagtc agagatatct tgcaacggtt ccaagtacga ggatataatg aagaaaaaaa
    14821 tgaaggacag cttcgtcaca tcattatccg tcgtggtcat tattcccatg aaatgatggt
    14881 cgttttagtt actcgtaaag aaaaattctt taaatccaag gaaattgctg aagctattca
    14941 tgaagaattg ccagaagtgg tttcggtcat ccaaaatatc aatgaagaaa aaacaaatgt
    15001 gattttagga gacaaagaaa aagttttata tggcagagac tatatcgaag atcagctatt
    15061 aggaaaaacc tatcgaatct cagcaaagtc attttatcaa gtaaatactc aacaagcaga
    15121 gtatctgtac caaacagcca tcgagtttgc cgagttagat aaaaaagaca ttgtgatcga
    15181 cgcttattca ggaataggta cgattggctt gtctttagca gacagagtgg ataaggtgta
    15241 tgggatggaa gttattccag aagcaattga agatgcacag ttcaatgctt taaataataa
    15301 aatcgaaaat gttcattatg aagtcggaaa agcagaaaaa gttatgaaaa aatggcaaga
    15361 aaaagggatc aaaccatctg tgatcgtagt tgaccctcca cgaaaaggat tagatcatca
    15421 ttttattgaa tcagcaatcg aaatgtcacc agaacgaatc gtctatatct catgtaatcc
    15481 ggcaactttt gcaagagatg ctaagttatt tgcagaagca ggttatgccg tgaagaaagt
    15541 tcagcctgta gatcttttcc cgcaaaccca tcatatcgaa ttagtcgctt cattcgttaa
    15601 aggagaataa aaatggacca aaaagaaaaa atgaaagaat tgatcaaaaa gaaacaaggt
    15661 ggaggccgat ccaaacaaat ggaaacacct aagaatgatc gtaaaaacat gagaaaaggc
    15721 cctaaaatct ttaataaata gacgtgctaa agaaagacta agaagtgtta acttttgtta
    15781 ttgtttctta gtcttttta
//
LOCUS       DAZUKD010000061        15745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000061 DAZUKD010000000
VERSION     DAZUKD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.062"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..626
                     /locus_tag="ACG8N9_001696"
     CDS             54..626
                     /locus_tag="ACG8N9_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4897274.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            771..1475
                     /locus_tag="ACG8N9_001697"
     CDS             771..1475
                     /locus_tag="ACG8N9_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4897275.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1703..2041
                     /locus_tag="ACG8N9_001698"
     CDS             1703..2041
                     /locus_tag="ACG8N9_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897276.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFNITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2026..2880
                     /gene="lgt"
                     /locus_tag="ACG8N9_001699"
     CDS             2026..2880
                     /gene="lgt"
                     /locus_tag="ACG8N9_001699"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4897277.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3027..3260
                     /locus_tag="ACG8N9_001700"
     CDS             3027..3260
                     /locus_tag="ACG8N9_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4897278.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3458..5362
                     /locus_tag="ACG8N9_001701"
     CDS             3458..5362
                     /locus_tag="ACG8N9_001701"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4897279.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            5716..6081
                     /locus_tag="ACG8N9_001702"
     CDS             5716..6081
                     /locus_tag="ACG8N9_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS4897280.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANED
                     ILSAIHVFLKLSENLSMKS"
     gene            6098..6711
                     /locus_tag="ACG8N9_001703"
                     /pseudo
     CDS             6098..6711
                     /locus_tag="ACG8N9_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
     gene            complement(6823..7143)
                     /locus_tag="ACG8N9_001704"
     CDS             complement(6823..7143)
                     /locus_tag="ACG8N9_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4897281.1"
                     /translation="MNSLSTSPLKNESIQEVSKIFKMISDPTRLSILFLLQKEELSVG
                     AIAHSLSMEQSAISHQLKTLKTSRLVKSKRAGKNMIYSLDDLHIFSILEQVSTHIEEQ
                     EKEK"
     gene            7399..9306
                     /locus_tag="ACG8N9_001705"
     CDS             7399..9306
                     /locus_tag="ACG8N9_001705"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4897282.1"
                     /translation="MKEIQEQHSHENNSHDHGKMPIILYFIGLALSLIALFVNGENNL
                     IQNTLFSIASISAGYHVIILEGIGETIENSKRQHKFTPNSHILMGIAAIGASLIGNFW
                     EGTLLVLIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLIMQDGNTTIVNVSELKVG
                     DQLQVLNGDQVPIDGVIVSGSTSIDESSINGESIPKEKSKGDGVFGSTINGTGTFTME
                     VTKETNDTVFSKILQLVNQNQENQTKASSIIQKFEPKYVKFVLIAIPLFILLAPMLFD
                     LTWSQSIYRGLVLLVAASPCALAAATVSVTLSATSNLAKRGVLSKGSAYLSQLADIQA
                     IAFDKTGTLTKGKPEVTNYYFSDSVNEENMIDIVVALEKESNHPLASAILDKFEPKNK
                     LAIEVENQIGKGLIGDYNENTYRIGKPTSFEAVSDDYTRLNKEWASEGNTVVYVSENE
                     KVIGLIALMDIPSEHAKATIDYFKKCGIHTTLITGDSEMTGQAVGKQLGVDQVIANVM
                     PEDKSNIINEQKETYGITAMVGDGINDAPALVKADVGIAMGEGTDVAVDVSDLVLMKN
                     DLSKLVKAHEISLKMNRVIWQNIIFSMVVVAFLITVSLVGLTDIAISVIVHEGSTLVV
                     ILNGLRLLKSK"
     gene            9400..9621
                     /gene="relB"
                     /locus_tag="ACG8N9_001706"
     CDS             9400..9621
                     /gene="relB"
                     /locus_tag="ACG8N9_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4897283.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            9622..9894
                     /locus_tag="ACG8N9_001707"
     CDS             9622..9894
                     /locus_tag="ACG8N9_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4897284.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(10025..12991)
                     /locus_tag="ACG8N9_001708"
     CDS             complement(10025..12991)
                     /locus_tag="ACG8N9_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS4897285.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            13137..13712
                     /locus_tag="ACG8N9_001709"
     CDS             13137..13712
                     /locus_tag="ACG8N9_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4897286.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            13926..14621
                     /gene="vanR-A"
                     /locus_tag="ACG8N9_001710"
     CDS             13926..14621
                     /gene="vanR-A"
                     /locus_tag="ACG8N9_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4897287.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            14599..>15745
                     /gene="vanS"
                     /locus_tag="ACG8N9_001711"
     CDS             14599..>15745
                     /gene="vanS"
                     /locus_tag="ACG8N9_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS4897288.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKR"
BASE COUNT         5059 a         2657 c         3201 g         4828 t
ORIGIN      
        1 aggaatcaaa gaaagctatt ttaaacaaac caaaattata ggaggaataa atcatgactg
       61 aaaataaagc tactcaagaa agaacacccc aagaaagatt gcaagaagaa caagaacaca
      121 aggaacatgt tcatcatacg aaaattaatg cagcggccat tgcggatcat cttttaggca
      181 acatgcatac attacatgtg aaactgcacc aatatcactg gtatgtaaaa ggagccaatt
      241 tctttacgtt gcatgaaaaa tttgaagagt tgtataacga aaatgaaaaa tggttcgaca
      301 aacttgcgga gcgtctaatt acttctgggc ataaacctgc ttcgacaacg gttgaatttg
      361 aaaaatattc gatgctttcg gaagatgcag ttaataaata cgctaaagca gaagacatgg
      421 ttgaaaatat tatcgaggac ttcagaagca ctcgtgacct aactattcgc gcgattcgtt
      481 tagctcaaga cgagggtgac gatgctttag aagatacatt gattgctttt aaaaatgact
      541 tagacaagaa catttggatg ctgcaagcgt tcattggtaa agaagcatta gaagatgatg
      601 acgctcttga tgaggatgaa gattaaggta aaagtagaag ctgatttagg gattggaatt
      661 ctttccagtc ctttttccat tttcttgatg tgcatcaatt tttttgtagt aagagactta
      721 tataataaaa gcagcgaata taaacaaaat aaataaaggg ggagaatgaa ttgactaaac
      781 atacccatca tgaaattggc gaccacgcag cctgtatccg cttggttcca atattcaatc
      841 acttagagga tagcgcgatg aattacattg ctgaaaaagc ccacacgaaa cagtatcaaa
      901 aaggagagtt tctgtttcgt tcacaagaaa aagaggatac tttatatatt atcaatcgtg
      961 ggaaaatccg tatttatcga ttggcagact ctggcaaaga gcagctagtg cggatcttga
     1021 accctggtga tttcaccgga gaatggacct tgttcaaccc ggatgcagta cacgaggaat
     1081 atgccgaagc aacccgagat accgttgtct gtatgattca gcaaaaagat gtccaagatt
     1141 ttctggaaca atacccagct atttctttaa aactattggc ggaaatgtct aaacggttag
     1201 aaagttcaga acgtcaaacg acacaagtag ccgtggagag tgtcattacc cgtttagttt
     1261 tgtttttggc agaaaatgtg gaacctgaaa tgggcaactc tcccaccatc accctgccga
     1321 tggcaaaaaa ggacattgct tcctatttag gaacgacacc tgagaccatc agtcgcaaat
     1381 ttggagaatt ggaggacgag ggattgattc aacagctgtc tgggaaaagg atcaagattc
     1441 aggatttaga tgatttattg ctttacagcg aataatcgga cacacttttt tctacacgtt
     1501 ggtgttctgt cgctcttcat taacaacggg acagaactca gtgcttttgc ttccatctgt
     1561 ccgtttttag cttttttaag acagaacgac cttcattcat tggaaaaata aaagcacaaa
     1621 catgataacc ctcaagtttt tttgttttat ttttctttaa gatagggttg tcgaacaaaa
     1681 cgtaaaaata agtagggtga atgtgcattg tttttgggtg atagttgcca tcacgttgtc
     1741 agcagcgggt gcattctttg tcgtcccttt caccaagaac acgattggcg gcgaagcaaa
     1801 aacgtctaac gttgttgagg cagataggtt agctatggaa aaagaagagg aagttgtaaa
     1861 aggagccggc gtaatcggac acggaaaagt ggcgtattac gaagcgagtg accagggaga
     1921 taaaagtgta ttcactgttt ggtttccatg gagcttagcc ggaatcttgt tcaacatcac
     1981 catgactaga atgacgcgaa aaattgaaga aatggagcaa atagaatggg attcgtatta
     2041 ggcgttttga atccccatgt ggtggagatt ggttctgttg tgattcattg gtatggagtc
     2101 attattgcag cgggcatatt agcggctctc cagttgagca ccaaggaagc aaagaaaaaa
     2161 ggactggaag aggataccat tatcgatatg gccttatggg caattccgat tggactcctc
     2221 ggcgctcggc tttattatgt gttatttgaa cttggttatt atctgcaaaa tccaggacaa
     2281 atccttgcga tttggaatgg cggcattgcg atttatgggg gattgattgc aggagggggt
     2341 acgctatatt ggtatgcaaa gaaaaaaggt acttctttgg cactcgttct tgatatttta
     2401 gcgcccaatg tattgttagc ccagtccatt ggtcgttggg gcaattttat gaatcaggaa
     2461 gctcatggtg ggccggttac tcgtcaattc ttggaaaatc tttatttacc tgaatttatc
     2521 attgaacaaa tgaatatgaa tggtacctac taccatccga cttttttata cgagtcattg
     2581 tggagtttgt tgggctttgt cctcatcgtc actatacgga accgaaaaca gcttttgcgt
     2641 caaggagaag tcgctttgag ttacgttttg tggtactcag tgggtcggtt cttcattgaa
     2701 ggtatgcgaa cggacagttt atggattggc gagtggatcc gcgtttcgca agccttgtct
     2761 ctggccctgt ttattggcgc gattgtagta tggtttatac gcagacaaga ttatccacca
     2821 atttcttatt attctgatgg caataagggg cagaaaaaga ctattaagat ggtgaattga
     2881 gcaaaaagat aaaaagaagt gcaaaacttg atatgcgtca attttcgttt gctaaaagtg
     2941 agctatacta cagttgtaaa gaggaaaagg cagtgttcat taatctgctg ccgataaaat
     3001 aaaaacacat aaaggagata ggaattatgg aaaaagctat attgcaatta gaaacgttgt
     3061 cttgtccaag ttgtatgcaa aaaattgaag gtgccgtgaa atcagttaac ggtattgata
     3121 aagacagcat taaagtatta ttcaactcca gcaaagtcaa aaccaatttt gattcagctg
     3181 tcacttcgat tgaagagatt caaaaagcta tcgaaaacgt aggctatccg gttcttaaag
     3241 caaaagtcaa ggccgtttaa cagattaaat gaactaggca attctggaag aaaagctatt
     3301 tttccaggat tgcttttttt gttgcaatcc gattcgaggt gcttagaaac gataaaagag
     3361 aactttctaa acttgatgtc agtcaattaa ttacttaggc aattatctta tactgtgttt
     3421 atcaataaaa gagaaattaa atttggaggg aaaaagcatg caacaatata tattaagcaa
     3481 gaaaaatgtt atcaccgtca tcagtggatt gttaatcgta ctcggtttct tcagtcactt
     3541 tgttttagaa aacgtaggac tttcagagtg gtctttgatc atcgcctctg tctttgggat
     3601 tacgccaatt gccattcaag cgtttcaagc aatgaaagtc aaagtcatca gtattgatgt
     3661 cttagtcagt attgcagcga ttggtgcgct ttttattcaa aattatgaag aatcggctat
     3721 tgtcacgttc ttattcttat tcggacacta cttggaacaa cgaacattga accaaacgcg
     3781 atctgctatc aaagaattga ctgaaatggc acccgaaagc gccttgaaac aaatggataa
     3841 tggcgaattt gaagaagtag aagtggatga tatagatgaa ggggatatct tgctcgttaa
     3901 aacgggtgcg aaagttccag tagatggcat agtccttacg ggtgaagggc atatcaatga
     3961 agctagcatt acaggtgaag ctattccggt caacaagcta gctgatgcag aagtttttgc
     4021 aggaaccatt ttagaaaacg gaacgattca aatcagagct gaccgagttg gtgaggacac
     4081 cacatttgga aaaattattg aactcgtgga agaagcacaa gattctaaat ccgaagcgga
     4141 acggttcatt gatcgctttt ctaaatacta cacaccagct gtcttggttc tggcaattgt
     4201 tgtatgggcg ttcagtcaaa atattgagtt agcaatcacc atcttggttc taggttgccc
     4261 aggcgcatta gttatcggag tgcctgtctc aaacgttgcc ggtattggga atggtgctcg
     4321 taacggtgtt cttttaaaag ggagtgaagt cattcaagac ttcagtaatg tggatacaat
     4381 tgtatttgat aagacaggta ctttaactgt cggaaaccca accgttgcag cgactgaact
     4441 gtatgaaaaa gatcctgctg aaacgcttgg ctatctggcc agtgtggaac gggaatcaga
     4501 tcatccatta gcaaaagcgg tcttaaatca aattggagaa acaaactttt atcctgttga
     4561 aggaactgaa gttgtgaaag gcggcggaat cgtttcaagt gtagctggac atagagtggc
     4621 tgttgggaat gtggccttga tggaaaaaga aaatgtcact ctcagcaaaa aagtgcaaaa
     4681 agatgtcaaa cggtttgaac aacaagggaa ctctctcgtt ttgacagcgg tcgacggtga
     4741 attaaaagta ctgatgggca ttcgcgatca agtccgtccg ggtgtgaaag ctaatttgca
     4801 ggaattaaaa gacttgggcg tgaaaaatct agtcgttctt tcaggagata accaaggaac
     4861 cgttgatgtg gtcgccggcg aacttggttt gaccgaagct cacggctaca tgttgccgga
     4921 agacaaatcg gcttatatcg gaaaacttca acaacgtggt caaattgtag cctttgttgg
     4981 agatggcgtt aacgatagtc cgtccttagc tttagcagac attggaatcg caatgggaag
     5041 cggaacggac gtagcgattg aaacatccga tgtcgtttta atgaactcga acttcagcaa
     5101 cttgcctcac gcattaggat tagtaaaagc caccgcaaat aatatgaaac aaaatatcgt
     5161 gatttcagtt ggagtagtgt tggtcttgtt gaccagcgtc ttcttcagcg aatggatgaa
     5221 tatgtctatc ggaatgttgg ttcatgaagg tagtatcttg gtggtaattt tcaatggcat
     5281 gagattaatg aagtataagt tgaaaggtcg gaaagaacaa aaagaagtat cagcacctac
     5341 agagaaagta aaaatatctt aagaataaat tagaagaggc tgagacaata gtccatcctt
     5401 gaaatgacaa aagcggaaac tcagattttt aaatctggga ttttccgctt ttttgagttc
     5461 ttgaaaaaaa ataagagtat agagtatgtt ctttataaaa ttaattttcg tgtaaattat
     5521 taaaaattta ataataaaat ttaaattcct attaagttgt aacacatgaa attgcttaaa
     5581 atagtgtatc aaaaaccttg ttttattaat gttgaattta tttgaaaggt ctacagatta
     5641 acatattatt gacaaaacaa tatttaaata gttatacttc attcaaacgt ctgtttgaac
     5701 gaggtggtga agttaatgac tgcagaagta tgtacaacta cttgtataca taaagaaaaa
     5761 gtagctagtg ttaaaaataa tttaaaagat aaagaccttt caactttact tgtattagga
     5821 aaatgtttta gcgattcttc taggattaaa attttctatg ctttagaaac gtataaggag
     5881 atgtgcgttt gtgatttagc agcgatactt aatgctagta ttgctacaac gtcacaccat
     5941 ttacgttttt tgaaaaagaa cggaatggcg aaatcacgtc aatatggaaa ggtagtttat
     6001 tactctttag cgaatgaaga catcctttcc gctattcatg tttttttaaa gttatcagaa
     6061 aatctatcta tgaaatctta gtttggagga agttattttg ttacaaagta ttttttcagc
     6121 tatcgctgtt tatatttcta ccagcattga ttatttgttt atcttgctga ttattttttc
     6181 tcaaagtcat acaaaaaagg gctccgtcag attttttggg gtcaatatct aggaacaggt
     6241 atcttagtag ccgtaagttt atttgcagca tatgtgctga atttcattcc gcaagattgg
     6301 atcattgggc ttctcggtct aattccgatt ttcttaggaa tcagagtggc attggttggc
     6361 gaagaggagg aagaagaaga ggaagtcgtt gaaaagcttg aatcaagagg aacaaaccgc
     6421 ttcttttgga ccgttgcctt aataacaatt gcttctggtg gcgataactt agggatttat
     6481 attccttact ttgcctcgtt atctttttca gagattgtca ccgctctaat cgtatttgct
     6541 atttctgttg cggttctttg ctatatcagt tacaaattag ccaagatttc ttttgtttcc
     6601 gaaaccttag aaaaatatga acgaatcctt gttcctatag tgtttattgg cttaggaatt
     6661 tatattttga tagaaaatgg gacgattcaa acactattaa attttatata aacaactacg
     6721 atagtgaagt tcaaataaaa aaagacagcg agattttaac tcgctgtctt tttttatttg
     6781 aactaatgaa ttgtaaaatc attttaacct tgtagtttgt ctttattttt ctttttcttg
     6841 ttcttcgata tgagttgaaa cttgctcaag aatactaaaa atatgaagat cgtcaaggct
     6901 ataaatcata tttttaccag ctctttttga ttttactaat cttgaagtct ttaaagtttt
     6961 taactggtga gaaattgctg attgttccat actcaaagaa tgcgcaatag ctccaacgct
     7021 aagttcttct ttctgcagaa gaaataaaat tgaaagtcgg gtagggtcac tgatcatttt
     7081 aaatatttta cttacctctt ggatagattc attttttaat ggagaagttg ataatgaatt
     7141 cataagaatt tctctccttc atatgtttgt ttattcacat ttagattatc ataatatttt
     7201 tataaagcaa gattttttgt ataagggtag ctattttaga gtcctatttt ttttatcacc
     7261 taaataagat aactattttt ttgacaaatt agatagagcg atataaaatg aatgtataga
     7321 catatgaatg ttcgttcaaa cgtaattcgt aagcattacg atttctaaaa aaaagtttta
     7381 ttaagaaagg tgatagaaat gaaagagatt caagagcaac actcacacga aaataatagt
     7441 cacgatcatg ggaaaatgcc aattattttg tactttattg gtttagcatt gtcgctcatt
     7501 gcgttatttg taaacggaga aaataattta atccaaaaca ctttgttttc aattgcttca
     7561 attagtgctg gttatcatgt cattattctt gaaggaattg gagaaacgat tgaaaactca
     7621 aaaagacaac acaaatttac ccctaattct catattttaa tgggaatagc tgcaataggt
     7681 gcttctctga taggaaattt ttgggaagga acgttattgg ttcttatttt ctctggagca
     7741 cattttcttg aagattatgc tgaaggaaga agtaaaagag aaattaccaa gttattagaa
     7801 atgaatccaa caacagctag gctaatcatg caagatggca atacaacaat cgttaatgtt
     7861 agtgagttga aagtaggaga tcaacttcaa gtgttaaacg gtgaccaagt accgattgac
     7921 ggagtaatag tatcaggctc tacttcgatt gacgaatctt ctattaatgg tgagagtata
     7981 cctaaagaga aatcaaaggg agacggagtt tttgggagta caattaatgg aacgggtacc
     8041 tttactatgg aagtcacaaa agaaactaac gatactgtct tttcaaagat tttacaacta
     8101 gtgaatcaaa atcaagagaa tcaaacaaaa gcgtctagta ttatccaaaa gtttgagcct
     8161 aagtatgtta aatttgtttt aattgcaatc ccactcttca ttttacttgc tcctatgctg
     8221 tttgatttga catggtcaca aagtatttat agaggattag tccttttagt cgcagcttct
     8281 ccgtgtgctt tagcagcagc taccgtatcc gtaacattgt cagccacttc taatctagca
     8341 aaaagaggcg tactttcaaa aggaagtgcc tatttgtccc aattagcgga tattcaagcc
     8401 attgcctttg ataaaacagg aacattaact aaagggaaac cggaagtaac caattattac
     8461 ttttctgatt ctgtgaacga ggaaaatatg attgatatcg tagtggctct tgaaaaagaa
     8521 tcaaatcacc cgttagcaag tgctatttta gacaagtttg aaccaaaaaa taaactagct
     8581 attgaagtgg aaaatcagat tggaaaaggt ttaataggcg attataacga aaacacttat
     8641 cgtataggta aaccaacttc ttttgaggct gtatcggatg actatacacg tttgaataaa
     8701 gaatgggctt cagaaggtaa tacggttgta tatgtatcag aaaatgaaaa agtaatcggc
     8761 ctgatagcat taatggatat tccaagtgaa catgcgaaag caactataga ttactttaag
     8821 aagtgtggta tccataccac tttaattact ggggattctg aaatgacagg gcaagctgtt
     8881 ggtaaacaat taggagtaga tcaagtaatt gcaaacgtca tgccagaaga taaatctaac
     8941 attatcaatg aacaaaaaga aacctatggt ataaccgcta tggtgggaga tggtataaac
     9001 gacgcacctg ctcttgtgaa ggcagatgtt ggaattgcta tgggagaggg tacggatgtt
     9061 gcagtagatg tatctgatct agtattaatg aaaaatgatt tgtctaaatt ggtaaaagct
     9121 catgaaattt ctttaaaaat gaatcgagtt atttggcaaa atattatttt ctcaatggta
     9181 gttgtcgctt ttctaattac cgtaagctta gtaggattaa cggatatagc cattagtgtg
     9241 attgttcatg aaggaagtac cttagtcgtg atattaaatg gtcttcgatt attaaagtct
     9301 aaataaatac atgaaattgg ataaaatcat gtatctaaag tttgcagcct ctctaatttt
     9361 gtattacaat gtatatacaa aaagaagggg gtgattctaa tgactgtcat atcgctcaga
     9421 attgatactc aggaagataa attaattaaa gaatatgcta aggcgaagaa tatttcggtt
     9481 tcggctctga ttagaagttc tgtcttggaa aaaatcgagg atgaaattga tttgggccta
     9541 tataatcaag ccatgaagga tcataaagag aatccgaaaa gtatttcttt tgaagacatg
     9601 ctaaaggagc tatctgatta aatgagctca ggttatcaag tcgagtttga aaaaggagct
     9661 caaaaagcgt taaaaaaaat ggacaaatac caatcccttt taattatggg atggattcaa
     9721 aagaatctag tgaattgtac cgatcctagg cagcatggaa aaggattaac cgtcaatcga
     9781 tcaggcgaat ggcgctatag aatcggtgat tatcgcttaa tagctgatat caatgacgag
     9841 accgtaacca ttttaatgtt agagattggt catagaaaaa atatttacaa ataaaccatt
     9901 caaattgagt gcaagcatgt ttaataaaaa aggattctac tgctttttaa ggggtagcgt
     9961 caggaaaatg cggatttaca acgctaagcc tattttcctg acgaatccct cgtttttaac
    10021 aacgttaaga aagttttagt ggtcttaaag aatttaatga gactactttc tctgagttaa
    10081 aatggtattc tcctagtaaa ttaatatgtt cccaacctaa gggcgacata tggtgtaaca
    10141 aatcttcatt aaagctacct gtccgttttt tatattcaac tgctgttgtt aggtggagag
    10201 tattccaaat acttatagca ttgataatta tgtttaaagc actggctctt tgcaattgat
    10261 gctgtatggt gcgttctcta agctcacctt gttttccgaa gaaaatagct cttgccaatc
    10321 cattcatggc ttctccttta ttcaatcctc tttgtatttt tcttcttaat gattcatccg
    10381 atatataatt caaaataaag atcgtttttt ctattcggcc catctcacgt aaggctgtag
    10441 ctaagctgtt ttgtcttgaa taggaaccta gcttccccat aataagggat gctgaaactg
    10501 ttccctccct tatagaatga gctaatcgca aaacatcctc ataattttct ttaatgacct
    10561 ttgtatttat ttgtccacgt aaaatggctt ctagttttgg atactcactt gctttatcta
    10621 tcgtaaataa ttttgagtcc gataaatccc ttattcttgg ggcaaattta aatcctaata
    10681 aatgagtcag tccgaatatt tggtcagtgt aaccggcagt gtctgtataa tgttcctcta
    10741 tgtttagatc cgtctcatga tgtaacaaac catccaaaac atgaatcgca tctcttgaat
    10801 tagtatgaat aatctttgtg tagtaagaag agaattgatc acttgtaaat cggtagatgg
    10861 tggctccttt tccagttcca taatgtggat ttgcatctgc atgtagtgat gaaacaccta
    10921 gctgcattct cataccatct gacgaagatg ttgtaccgtc gccccaatag aaaggcaatt
    10981 gtaatttatg atgaaagttt actaatatgg cttgggcttt attcatggca tcttcataca
    11041 tgcgccattg agatacattg gctagttgct tatatgtaag tccgggtgtg gcttcggcca
    11101 tcttgctcaa gccaatattc attcccattc ctaaaagggc agccatgata atgattgttt
    11161 cttccttatc tggttttcga ttattggaag catgagtgaa ttgctcatga aatcctgtta
    11221 tatgggccac atccatgagt aaatcagtta attttattct tggtagcatc tgataaaggc
    11281 ttgcactaaa tttttttgct tcttctggaa catctttttc taagcgtgca agtgatagct
    11341 ttcctttttc aagagaaacc ccatctaact tattggaatt ggcagctaac cactttaacc
    11401 tttcattaaa gctgctggtt ctctccgtta tataatcttc gaatgataaa ctaactgata
    11461 atctcgtatt ccccttcgat tgattccatg tatcttccga aaacaaatat tcctcaaaat
    11521 ccctatattg tctgctgcca acaatggaaa catctcctgc ccgaacatgc tcccgaagtt
    11581 ctgttaaaac agccatttca tagtaatgac gattaattgt tgtaccatca tcctcgtata
    11641 aatgtctttt ccatcgtttt gaaataaaat ccacaggtga gtcatcaggc acttttcgct
    11701 ttccagattc gttcattcct cggataatct caacagcttg taaaagtggc tcatttgcct
    11761 ttgtagaatg aaattccaat actcttaata gcgttggcgt atattttctt agtgaataaa
    11821 accgtttttg cagtaagtct aaataatcat agtcggcagg acgtgcaagt tcctgagcct
    11881 cttctactga agagacaaag gtattccatt caataaccga ttctaaaacc ttaaaaacgt
    11941 ctaatttttc ctctcttgct ttaattaatg cttgtccgat gttcgtaaag tgtataactt
    12001 tctcatttag ctttttaccg ttttgtttct ggatttcctc ttgagcctta cgaccttttg
    12061 ataacaaact aagtatttgc ctatcatgaa tttcaaacgc tttatccgtt agctcctgag
    12121 taagttgtaa taaatagatg gttaatatcg aataacgttt attttcttga aagtcacgga
    12181 atgcatacgg ctcgtatctt gagcctaagc gagacagctg caacaggcgg ttacggtgca
    12241 aatgactaat ttgcactgtt tctaaatcca ttcctcgtat gtattcgagt cgttctatta
    12301 tttttagaaa agtttcgggt gaaggatgac ccggtggctc ttttaaccaa cccaatatcg
    12361 ttttattgga ttcggatgga tgctgcgagg taataatccc ttcaagcttt tctttttgct
    12421 catttgttag agatttacta accgtattaa atagcttctt ttcagccatt gcccttgctt
    12481 cccacaccat tctttcaagt gtagtgatag caggcagtat aattttgttt tttcttagaa
    12541 aatctatgca ttcatgcagt agatgaatgg catcaccatt ttccaaagct aattgatgaa
    12601 ggtacttaaa tgtcattcga tattcactca gggtaaaagt tacaaagtcg tattcacttc
    12661 gaatttcttt caaatgatcc caaagtgtat tttccctttg aggataatga tcaagcgagg
    12721 atggactaac accaatctgt ttcgatatat attgtatgac cgaatctggg atgcttttga
    12781 tatgagtgta tggccaaccg ggataccgaa gaacagctaa ttgaacagca aatcctaaac
    12841 ggttttcttc cctccttcgc ttattaacta tttctaaatc ccgtttggaa aaagtgaagt
    12901 aggtccccag tatccattca tcttcaggga tttgcataaa agcctgtctc tgttccggtg
    12961 taagcaattc tctacctctc gcaattttca ttcagtatca ttccatttct gtattttcaa
    13021 tttattagtt caattatata tcaatagagt gtactctatt gatacaaatg tagtagactg
    13081 ataaaatcat agttaagagc gtctcataag acttgtctca aaaatgaggt gatattttgc
    13141 ggaaaatcgg ttatattcgt gtcagttcga ctaaccagaa tccttcaaga caatttcagc
    13201 agttgaacga gatcggaatg gatattatat atgaagagaa agtttcagga gcaacaaagg
    13261 atcgcgagca acttcaaaaa gtgttagacg atttacagga agatgacatc atttatgtta
    13321 cagacttaac tcgaatcact cgtagtacac aagatctatt tgaattaatc gataacatac
    13381 gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta tcagaagata
    13441 atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta gagcgagatc
    13501 ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga aagtttaaag
    13561 gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta aagctatata
    13621 aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat
    13681 tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata
    13741 tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc
    13801 cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc
    13861 ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga
    13921 taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg
    13981 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
    14041 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
    14101 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
    14161 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
    14221 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
    14281 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
    14341 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
    14401 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
    14461 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
    14521 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
    14581 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
    14641 ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg
    14701 ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa
    14761 aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac
    14821 aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc
    14881 atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt
    14941 cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc
    15001 aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga
    15061 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
    15121 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
    15181 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
    15241 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
    15301 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
    15361 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
    15421 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
    15481 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
    15541 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
    15601 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
    15661 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
    15721 atgccagact tggttgataa aagga
//
LOCUS       DAZUKD010000062        15013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000062 DAZUKD010000000
VERSION     DAZUKD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.063"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1480
                     /locus_tag="ACG8N9_001712"
     CDS             56..1480
                     /locus_tag="ACG8N9_001712"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4897289.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1779..2486
                     /locus_tag="ACG8N9_001713"
     CDS             1779..2486
                     /locus_tag="ACG8N9_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4897290.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2473..2796
                     /locus_tag="ACG8N9_001714"
     CDS             2473..2796
                     /locus_tag="ACG8N9_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4897291.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2885..3922)
                     /locus_tag="ACG8N9_001715"
     CDS             complement(2885..3922)
                     /locus_tag="ACG8N9_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4897292.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4243..5226)
                     /locus_tag="ACG8N9_001716"
     CDS             complement(4243..5226)
                     /locus_tag="ACG8N9_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4897293.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5641..6411)
                     /locus_tag="ACG8N9_001717"
     CDS             complement(5641..6411)
                     /locus_tag="ACG8N9_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897294.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6404..7339)
                     /locus_tag="ACG8N9_001718"
     CDS             complement(6404..7339)
                     /locus_tag="ACG8N9_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897295.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7655..9304)
                     /locus_tag="ACG8N9_001719"
     CDS             complement(7655..9304)
                     /locus_tag="ACG8N9_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4897296.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9421..12024)
                     /locus_tag="ACG8N9_001720"
     CDS             complement(9421..12024)
                     /locus_tag="ACG8N9_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4897297.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12036..13086)
                     /locus_tag="ACG8N9_001721"
                     /pseudo
     CDS             complement(12036..13086)
                     /locus_tag="ACG8N9_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(13112..13426)
                     /locus_tag="ACG8N9_001722"
     CDS             complement(13112..13426)
                     /locus_tag="ACG8N9_001722"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4897298.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13442..13888)
                     /locus_tag="ACG8N9_001723"
     CDS             complement(13442..13888)
                     /locus_tag="ACG8N9_001723"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4897299.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(13889..>15013)
                     /locus_tag="ACG8N9_001724"
     CDS             complement(13889..>15013)
                     /locus_tag="ACG8N9_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4897300.1"
                     /translation="KAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVE
                     LQELVRIKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMIS
                     VDLLEEIEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQR
                     INNGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKV
                     KQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGA
                     IKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVK
                     PELNKIYETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4435 a         3081 c         2525 g         4972 t
ORIGIN      
        1 tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa aaattatgac
       61 aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt ggtcagctga
      121 gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc gctggtttaa
      181 agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg atcacatcca
      241 tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct ttcgcgtttc
      301 tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc ccaaagccat
      361 ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag tatttgccaa
      421 cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg aatcacgtga
      481 agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg gtgacttagt
      541 ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt atcttgaaga
      601 ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat tcttcactat
      661 acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta gtaaaatagg
      721 cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg atgtgaaagc
      781 agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg ttaaagggaa
      841 tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg aagattatac
      901 gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac taaactatta
      961 cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc catttttacc
     1021 agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat accggggatg
     1081 ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc attatggaaa
     1141 catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac gtttcatgga
     1201 tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc atcttgctta
     1261 cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt ggacatttat
     1321 tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt cactcgacct
     1381 tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat taagtgatac
     1441 aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa ttccacttga
     1501 agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac tttttcttca
     1561 tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc ctcagaagtt
     1621 aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt ttttatttgt
     1681 ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag tttgacgaca
     1741 attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag ttagatataa
     1801 aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt ggacttgcat
     1861 ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg atgtccctgc
     1921 tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc catagtccac
     1981 tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg atgagtatga
     2041 cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt ggcacattgc
     2101 tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga caaaagttaa
     2161 actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg ggttcttctc
     2221 ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt ttggatttcg
     2281 ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat agaaacacct
     2341 ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat gtcggactga
     2401 tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc ggattggggg
     2461 tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg ttgtacttta
     2521 gcaacttggc tttctaggat cctaccattt gtcttactga aaaaattcga tttgccgcaa
     2581 ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt atggttcagc
     2641 agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa tgctttggct
     2701 tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat cgtcttggta
     2761 ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga gcttatggca
     2821 aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat cggcgtattc
     2881 ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt cctgtttcta
     2941 cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag accttgctca
     3001 atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa gcttctcctt
     3061 gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag tcgaagctga
     3121 tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg atcgttccca
     3181 catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta atatcgaata
     3241 atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta cttaagtcgt
     3301 gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa ttcttcggac
     3361 ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa atcgaaccga
     3421 cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt tcttctggag
     3481 tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac ggcaaggctg
     3541 cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg tcaaccagtt
     3601 ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg cgatcgccga
     3661 cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca tcaaacccta
     3721 agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg acaggattaa
     3781 cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt atctgttctt
     3841 tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa ccgactacct
     3901 tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt ggagcatcca
     3961 tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt catcataatt
     4021 attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag
     4081 acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt attcgctcaa
     4141 aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt gatgcaggac
     4201 agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag
     4261 cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag tagatttcgt
     4321 attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat agattttaaa
     4381 gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat cttgcagatc
     4441 tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg gttctttttc
     4501 aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta cgccatattt
     4561 ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg tgatcagtgt
     4621 ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc aatgtgcatc
     4681 tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta tatcttgatg
     4741 aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga tacgcgtctt
     4801 gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg ctacttctgg
     4861 gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg ggcagcccat
     4921 gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt ctctaaaaaa
     4981 ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg taaaggtcaa
     5041 tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc
     5101 agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata caacatctgt
     5161 tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc aaaaattagt
     5221 agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa
     5281 cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc
     5341 tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc cggaataaat
     5401 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca aacgaaaaat aggaggagct
     5461 atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact
     5521 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacgacaaa atatgaggaa
     5581 ctattttccc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt
     5641 ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac
     5701 gatgaatagc acaagtacga tcatcgatgt tgctactggc acatcgattg ttccaaggaa
     5761 tccgtaacgg aaaccgaaga tcatataaac gatcggattg actttcgaaa cagcttgcca
     5821 aaatggcggc aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa
     5881 gacaaatgta ggcacgatcg atacatcgtc ataagactga gcaaaaatcc cgttgagcag
     5941 tcctgctaac gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga
     6001 atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat
     6061 actgcgaccc actccgccaa tcacaaagcc ccaaatgatc acatgcgtag gaacaggcgc
     6121 taccaagatt tcttcgatgt ttttctggaa tttttgagag aagaaggacg aagaaacgtt
     6181 ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggcacgatga attccatata
     6241 tgaaaaacca cccatgtcac cgatccgacc accgatcatt tttccgaaaa taacaaagta
     6301 caaggatgtc gtgatcacag gcggaaccag tgtttggacc cagatacgca aatagcggtt
     6361 cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct
     6421 ccgtatgatt attttcttct gtgattttca aaaacagttc ttctaaacga ttcgacttat
     6481 tcctcataga aagaactttg attccttgtc ttgtcagctg atcgaagatt ccgttcaccc
     6541 cttgatttcg ttcaacttcg acagcgattg tctgttcatc ttcaaatgta caagcgtatc
     6601 cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca
     6661 gtttggatag aagctctttc atactagtat tctcgatcag ttcacctgat tggatgatcc
     6721 cgatgttgcg acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa atgatcgttg
     6781 taccattttc gttcaattct cggagaaact cccacatctc gcgtctcaat tcaatatcga
     6841 ctcctgctgt tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa
     6901 tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc
     6961 aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac
     7021 cgtagtagcc tgcttgattc acgacgattt gctggaccgt ttcaaaggga ttgaaattga
     7081 actcttgcgg gactaatcca atctgttgct ttgctcggac tagatccgta tccagatcat
     7141 aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaatg attccgatcg
     7201 ttgtagactt cccagctcca tttggtccta atagcgcata gaaatctcct tctttcacgg
     7261 aaagatcgat tcctcgcaat gcttcgaccc cacttccata aacctttttc aaattattta
     7321 tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct ttattgtatc
     7381 atggaataca gcagatgaaa aaacaacttg gttccagcga aaaatcaggt tgctgggctc
     7441 aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc agacaacctt ttcagaaaaa
     7501 acaaatcgaa gataaaggat tactattcat atgtgcaaca aaaggagcgt gagacaaaag
     7561 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca aaagccaaga
     7621 tttttgatcc aataaatact ttcgatcact tcgcctatcc caatacatgc cactgccgct
     7681 tttcatcaaa taacgcggct tccgtttttg cggtttctac taaagtctct gggtaatcca
     7741 aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt aatttgaaat
     7801 caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc tcacaataat
     7861 tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa gatggatact
     7921 ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta tttgtccctt
     7981 ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg tccaacaaac
     8041 gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga
     8101 ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt
     8161 ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta gattttcctg
     8221 atgcattgga acccgtgact aatgtattct tctgccagtt gaccggattc actaccgctt
     8281 cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta aactctggtc
     8341 ggcaagtgat gggcatataa gtacggtaat tcaatattgc agcggctact tctaattctc
     8401 ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta gtgagaacga
     8461 aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa atcaactctg
     8521 cttcactccc actcttcata cgaaatgaaa aacctaattt agggatgtct cttaatggtt
     8581 tagtttgctg tttcagctct tcttgaagtg gtgtcggcag ttttgctagc tgattggcgg
     8641 ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt gtttgtttct
     8701 tcactagata atagaccgta ttgaatacta acccaccgat tgccaaatag attcctagta
     8761 cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag caagccaaga aaaataaata
     8821 aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt gcaaaattat
     8881 gatctttttt tcctaaacga gcgaattgat attggatttt ttctctcagt tgaggatttt
     8941 cctgataaaa agcaatcagt ttttctaact gctgatcttc accaaaatca aagctgcgta
     9001 aacgttgata caacgcttct gaacctacac tagaataggt actgttgatt ccttcgaata
     9061 cttcgaacat atccaggtca taccaagtaa gatcatcgat ttcgctatcc catgaacgga
     9121 acttcttctc cgtgagccat gcttccttca agctttcttc tttatcaaat ctcggttgat
     9181 aaggaatttt ttcccatttg ctacgaactg tttcctttaa tttcaaacgt gaaaaaatgt
     9241 ctaaagcaac taataaaata acagctgcaa taataattaa aacgataaat tctctttgac
     9301 ccatatccct ctccgttcct ttatttgtta gctttagtat agcactgttg cgctatttga
     9361 aaagagggta aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta tattacatat
     9421 ttatctctca tttatcaaaa ttgacaaata atcataggga ggaattgttg ttaattcttg
     9481 tttattctcc agtgcattga caactgtccc aagggaaagg atagcctcag gtactatttt
     9541 ttcttgttct gttggattag ctaagcgaag aacaaatcct tgttcatgaa aataagaagg
     9601 ataaatcgat gatacaatta agtcttcagg taattcaatc aatgaaaatt tacgaggaac
     9661 atcaagttca ttttgtaatg gccaaatctt gttatctaac ctgtgaataa atacattcag
     9721 tttctgcttt tgataagaaa tagattgttc taaccgtttt tctgcctgtt ttgccacttt
     9781 gaactcatta aatcttcctt cttctacatt aaatccaaaa gtgactttat actcacctat
     9841 ctcttgagct agcggtgttt ccatcatgac atgcccttca tttgtcgtat ctcctgaagc
     9901 tcgcccaggt cgccaagcaa gattaggttt tccaagttcc cctgttgttg aaaataaggt
     9961 tatgattaat tgattttcta tacgttcata ctcttttaaa ccatctgcat aaaacgtaaa
    10021 ataatgatca ttttctgcca cagagacact cttatcgaat atttctaaat taacaggttt
    10081 ctctaccata gtttctgacc aattcggagg aatgacatca ggggtatttt caataaatcc
    10141 gttttgaatt tgtgcaattg atttttcaga tttatctaaa acattaattg ctaagcgcat
    10201 acgatgcgag taaacagtat tatgaatttt gagtgtgcct tcaacgattt gactttctcc
    10261 cagactgaac gatatttcat aagatatttc ttccatttcg ggtttttcag ataaacgatc
    10321 ttttaaatct ttaggaagtt gagcttttcc gtaaacaact aatgtttctt gtaaagaatc
    10381 tttatatact ttagctgttt ctaattttag agatagttct gtatctcctt ctaatggtga
    10441 ataatcatac gtatcgccat catttgctga atcgatcaaa tgaacgaaat caagatattg
    10501 tctatctcca actatcaaat tcaattgccc atctttaaaa gaaagtttat atttattgtt
    10561 acttatctca gttttgtcta tctcgattgc tgtttctaat ttttcattat ttttctcaaa
    10621 ttcaacaaca gtgtatccta gtgctggcac atttacattt atttttatgt caagttcata
    10681 ataagctggt tcatcaaaat attcgaaacc tgtagcaatc atccgataag catttttccg
    10741 ttctggaaaa taaacttctt tttcaatgat attataggga ctacctttga agtgtatttt
    10801 ctttgaatca gctactacat gcacgatttt cgatccacta taattctcag tcgttgtgtt
    10861 gaaaacaagg acttcattcg attttagctt taatgcatca gccatccgtt ttacaataag
    10921 gttttcgatt ccgtcagcta tctcattagc ttctttgatt cgatggaaaa tatcttccgc
    10981 aacattatct gaaacacagc caccaatact atcatgagcc tggttttgca aaatcttttt
    11041 ccataaccga cgaagtaggc ctttactgat atggatacca tttctttctg caatcagcat
    11101 gagcggctca actcgtttta gaattttttg ttccaattta aaattttcta ttttcatttt
    11161 tgttcttact gagccaatag aacgatgaac acgagagtaa accggctgac gaagttcacc
    11221 tttatattca ggtaattctt ttttgtttct gataacttct acataagaag gataatctga
    11281 aataatattg tggaatttac tgtatttatt caacttctct acgacttgat caaaatttaa
    11341 aatcattgac ttttgatcta cacccgaagg tactaaaata tctttgtttt ttccttgcat
    11401 atgaagcatt tcaactgctt catccaactt ttcttttgca aattcaggaa tatctttaat
    11461 tgcgtctaag gtcatcattc cagtcatata accaaatgga aaattcatgg cataaacaaa
    11521 tttatctccc aatcctttcc agataaaata aggagatgga accatcgttt caaagtttaa
    11581 tcctcgccaa cacatgaaat tatcgatttc tacttgatgc aaaagtgtag gcaaattcgc
    11641 attgaaccca aacgtgtcag gcaaataccc taccatcatt ggatctccat atttaaggat
    11701 cgtatcactg attccaatca ttaagtttct taaaattgat tcagcatcca ctaataaggc
    11761 atctgtttgt gcataccacg gtcccacgaa tagttgtcct gttttcatca gtcgttggat
    11821 aaccttaagc ttttgcggat tgatctccaa atactcatcg acgattgaag tttgtccatc
    11881 taaacaaaag tttactttag ggttcctttc caattcatct agaacttcag taaagacctg
    11941 atcagacaat acgatggcat cttcatttgt aaaataccat tctctgtccc agtgcgtgtg
    12001 ttgaattata tgtgcattta ccatatcaga ttctcctact ttccttcttc tactgaattt
    12061 acttgttgtt cttttttata cttgatggat tttagtgcat tgaccattaa cgctgtaacc
    12121 actcccccaa taacaattga tactaaatat aaaggtatat tcgtaacaaa aggaattgcc
    12181 ataaaggttg aaagcattgt ttcttgagta acaccaaaaa atccatttag accacatgct
    12241 actgcagtgc ccaccacgat gcttggtatt actcgtaaag gatctttcac ggcaaacggg
    12301 atcgtccctt cagtaatccc cacaaggccc ataatcatag ctgtctttgc attatctcgt
    12361 tccgattcat cataaagttt tttgtttaaa attgttgcta aaaacattcc aaatggcgga
    12421 atagcaattc ctaataacat ggctgtacta ggtccaaagc ttccgcttga aaaagaagca
    12481 accccaaaag catatgcgat tttattgata ggtcctccca tatcaaacgc catcattcct
    12541 cctaaaatag ccgacatcaa agcagcattt gctcctgaca tgccttctag ccaattagtc
    12601 agtcctgttg tcattgcttg aataggcgta gctagtacat accataatat gattccaaca
    12661 actaatgtag taacaaaaag gaatcagcat aattgatttt agactaataa cttgcatcgg
    12721 tacttttact tttttcaatg cattagctaa atagccacaa gctaatccag caaccattcc
    12781 tccaataaat cccataccaa gattttgaca catcatccca gtgataaaag ctggtgctag
    12841 accgggacgg tcagcaattg aatatgcgat ataggctgaa atcatcggta cgataaactg
    12901 taatcccgtc tgaccaatca ttcgcaggac tccccagaat gtatccgtct ggtcccaaac
    12961 acctgtgcca ccaaaaatga ctgcaataga agttagtaac cctgctgcta cgacaagcgg
    13021 gatcatatag gaaaccccac tttggaaatg atttagaatt tctcttcctt gtttcttgaa
    13081 ttccattagt aactccccca cgatatattt tttatttgtt aattaatgca attgcttcat
    13141 caatcgtctc ttcaccatct tttactacct ctgaggtccc tactgttaaa atcggtttac
    13201 cgtcaaagcg acctcgttct gaaacatttg tatctactgc aaatatgaca acatctgcct
    13261 cggaaacatc cgaaattgaa agtttgtttt ctatccccat agccccctgt gtttctactt
    13321 taatatctaa ccctttcttc ttcgcaaact tttctaaatt agcttttgcc atataagtat
    13381 gggcaatccc tgcgatacat gctgttactg ctactatttt tgtcatatga aattcctcct
    13441 tttaaaatga tagagctttt accaattctt ttttatttct cgaatctttt attcttttaa
    13501 caaactcgtc atccatcaat cttccagata attctgctaa aatacgtaaa tgattagtac
    13561 ctttatcagt gtcttttatt gctaacaaaa agacgagatc aactggttga tcatctaatg
    13621 cgccccattc aacagaccgc tttaacttgg ctacagccac tgttgattca attactgcta
    13681 ggcttttgcc atgaggaatg gcgatatttt tcccaattcc tgtggtcata tggttttctc
    13741 tgtcaaatac gtctttttga aattgtttgt ggtcagacac aaacccatta gtctctaaca
    13801 atttcgataa tttttcgact acactttttt tatcagatgc atttaaatct atcactatat
    13861 tatttatatt gattttttca ctgatcattt ataagccttc tttcaatata taaactaatt
    13921 tttcaaacga cgaactcgtt tttaaatttg ctaataactc atgattcgaa gcgatttcat
    13981 tgaatgtttc ataaatttta ttcaattcag gttttacttt ttcattaagt gcaaaaaaga
    14041 aaacagaatg aacaagtgtc ccatcccaat caattccttc tggattaacg tagacacaga
    14101 ttactggctt ttttataaaa tctacttcga tatgcggcat agcaaaatca ttcatcgctg
    14161 ttgaagatag tttttctctt ttgattgctc cctctagtat cccttctttc gcgtaatttt
    14221 cttttaccaa ttttttggta attatattta aaacgttcac ttgattatcc tttatttggt
    14281 gggtaaaaaa gcaaaatcgt ttatctaata aatttagaaa agcatcttct gtttgttcat
    14341 ttaactgtag atactgtttt acttttctga catctgcccc taacatcaaa ggactaacaa
    14401 caataacagg aaaatttgta ttttcaatcg gaatcgtgct tataacaagt tcatcagtga
    14461 cttttctatc ttgtagttca ttgatgctta gtacatcttc tatctcaacc atatctgcaa
    14521 aaccgttatt tattctttgc tctaataatt ttgatgttcc atatccgcta ctacaaacta
    14581 atatcacttt cgttttgtca ggtttgtaac gatccaaaag tgattgaata tggagtgtta
    14641 cgtaagcgat ttcatcttca ttaaaatgta tacagaatct ctcttcgatt tcctctaata
    14701 aatctacact tatcataaat gaccttttaa aagaatattt gattttgtcc gtataagggt
    14761 tatagatatt tactcccaat ttgagtcttt taactgctgc attgagatgt aatactagat
    14821 tattgatcaa ttcttcatca ggataaaatt catttaattt aattcttact aactcttgta
    14881 gctcaacacc catatctaaa taggaaagct tcgtttgatc taagtcgtga ttcatagcat
    14941 ttttttcaat cgaagtaatg tgaatgtcaa cataagcttg ttcatctatt ggcacaataa
    15001 ttgaaaatgc ttt
//
LOCUS       DAZUKD010000063        14815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000063 DAZUKD010000000
VERSION     DAZUKD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.064"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8N9_001725"
     CDS             complement(296..1024)
                     /locus_tag="ACG8N9_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897301.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8N9_001726"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8N9_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4897302.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8N9_001727"
     CDS             2805..3743
                     /locus_tag="ACG8N9_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897303.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8N9_001728"
     CDS             3755..4666
                     /locus_tag="ACG8N9_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897304.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8N9_001729"
     CDS             4690..6162
                     /locus_tag="ACG8N9_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4897305.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8N9_001730"
     CDS             6213..6824
                     /locus_tag="ACG8N9_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4897306.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8N9_001731"
     CDS             6854..8767
                     /locus_tag="ACG8N9_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS4897307.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8N9_001732"
     CDS             8895..11876
                     /locus_tag="ACG8N9_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS4897308.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8N9_001733"
     CDS             11873..13534
                     /locus_tag="ACG8N9_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS4897309.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8N9_001734"
     CDS             13574..14506
                     /locus_tag="ACG8N9_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4897310.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZUKD010000064        14696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000064 DAZUKD010000000
VERSION     DAZUKD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.065"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..1081
                     /locus_tag="ACG8N9_001735"
     CDS             77..1081
                     /locus_tag="ACG8N9_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897311.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1082..2044
                     /locus_tag="ACG8N9_001736"
     CDS             1082..2044
                     /locus_tag="ACG8N9_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897312.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRRVYDLRTITTTHKVALKDGGAS"
     gene            2044..3006
                     /gene="opp4B"
                     /locus_tag="ACG8N9_001737"
     CDS             2044..3006
                     /gene="opp4B"
                     /locus_tag="ACG8N9_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4897313.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3033..3947
                     /locus_tag="ACG8N9_001738"
     CDS             3033..3947
                     /locus_tag="ACG8N9_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897314.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3973..5754
                     /locus_tag="ACG8N9_001739"
     CDS             3973..5754
                     /locus_tag="ACG8N9_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4897315.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6102..6788
                     /gene="rnc"
                     /locus_tag="ACG8N9_001740"
     CDS             6102..6788
                     /gene="rnc"
                     /locus_tag="ACG8N9_001740"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4897316.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6808..10389
                     /gene="smc"
                     /locus_tag="ACG8N9_001741"
     CDS             6808..10389
                     /gene="smc"
                     /locus_tag="ACG8N9_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4897317.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10398..11207
                     /locus_tag="ACG8N9_001742"
     CDS             10398..11207
                     /locus_tag="ACG8N9_001742"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4897318.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11218..12216
                     /gene="ftsY"
                     /locus_tag="ACG8N9_001743"
     CDS             11218..12216
                     /gene="ftsY"
                     /locus_tag="ACG8N9_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4897319.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12403..14670
                     /gene="gshAB"
                     /locus_tag="ACG8N9_001744"
     CDS             12403..14670
                     /gene="gshAB"
                     /locus_tag="ACG8N9_001744"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4897320.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT         5025 a         2645 c         3229 g         3797 t
ORIGIN      
        1 taaaacgatt tattctgtgt aatgtccttt tttattaaga aaaacttcca tttaggaagc
       61 agagaggggt attcgattgt ccgataacca attattagaa gttcagaacc tacatacagg
      121 attccgactc aaagatgact attacgatgc agtagatgac gtttcattta ctttagacaa
      181 aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct tggctactac
      241 gattatggga ttacttgatc ctaataacac aaaaattaca ggtgagattt tatataacga
      301 tctgaattta atcgacctga acgaaacgct ctataataaa atccgcggaa atgatatcgg
      361 aatgattttc caagatccgt tgtctgcatt aaatcctctc atgagaatcg aagaccagat
      421 caaagaagga ctttcctatc acacaaagat gaatgcagaa cagcgtcaag ctcgtgcact
      481 agaattgcta gaacaagtag ggattcctaa tcctgcgaga gttggacgcc aatatcccca
      541 tgaactatca ggcggtatga gacaacgtgt catcatcgca atcgcaatcg cctgcaaacc
      601 tccaatcgtc attgcagatg aaccgacgac agccctagat gtaaccatcc aagcacagat
      661 tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct tgatcactca
      721 tgacttagga gtcgtagcag aaatggctga tcgagtggca gtgatgtatg gcggacagtt
      781 cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt atacacgctc
      841 tttactgaac tctataccac aagaaggaaa tcatgatgca gaactccatg tgatcgaagg
      901 tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt cggtttgcac cacgtattcc
      961 atggatacca gcaagcgatc atgaagaaga gcctatcctt catgaagtcg caccgaacca
     1021 cttggtacgt tgctcctgct ataagcattt ccactttaga gacgaaaaag gagaggtgta
     1081 gatgacagaa ttaatcacaa tcaaagacct gaaagttcat taccctattc gcagtggatt
     1141 cttcaatcga gtaacggatc atgtatatgc tgtagatggt gtggatttta ttatcgaaaa
     1201 aggaaagaca tatggtttag tgggagagtc tggctctgga aaatctacta cgggaaaagc
     1261 cgttgtggga ttagaaaagg taacagccgg acaaatcatg tatgaaggaa aagatgtaac
     1321 gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat gtgcagatga tcttccaaga
     1381 ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat atcattgctg aacccatccg
     1441 aaattttgaa cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac tgctagatat
     1501 cgttgggatg ccggaagatg cgctatataa atatcctcat gaattttcag gcggacaacg
     1561 tcaacgtcta ggagtagccc gtgctgttgc taccaatcca aaactgatcg tagccgatga
     1621 acctgtatct gctctggatc tatctgttca agcgcaagta ttgaatttca tgaaacgaat
     1681 ccagcaagaa tacggcctca gctatttatt catttcacat gatttaggtg tagtaaaaca
     1741 catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg gtacaagaga
     1801 agacatttat aatgacccgc gacatatcta tacgaaacgt ctgctttcag ctattccgca
     1861 aatcgatgta gaaaataggg aaagccacaa agaaaatcga cgaagagtag aacaagagta
     1921 tatccaaaac caaaaagaat actatgatgc gactggtcga gtctacgatt tgcgtagagt
     1981 ctacgatttg cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag
     2041 ctaatgtgga aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta
     2101 agtgttctga tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc
     2161 aatccaaatc aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac
     2221 ccatggtatg tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa
     2281 agtttcttat acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta
     2341 tatctatcga tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt
     2401 gctggccggt atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt
     2461 tttgctgttc ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta
     2521 ggctggttcc caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa
     2581 tggatagacc gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact
     2641 gccgtcacaa tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc
     2701 agaacagccc gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga
     2761 aatgcagcgt tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt
     2821 tctgtagtta tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc
     2881 atcggccaac gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg
     2941 ctagtgggga cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt
     3001 caataaaacc aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc
     3061 aagaaagtat tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa
     3121 ttgctatttt ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga
     3181 tgctgaccga tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag
     3241 gaacagtttc gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg
     3301 tattaggaca attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga
     3361 tccttacttc gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca
     3421 ttgacaacat cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca
     3481 ttatcgtctt cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga
     3541 gtgcatttta ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa
     3601 gaagagatta tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc
     3661 gcgaaatcat gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg
     3721 caaacatcgg tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac
     3781 ctagtttagg tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat
     3841 ggatctggtt acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg
     3901 gacaagcatt caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag
     3961 ggggaaatag gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt
     4021 cttgcagcat gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc
     4081 aataacaaag aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa
     4141 gacggcgaat tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg
     4201 gagttttacc aagattcata tgatgcagct tttatggctc cttctcatga accgctattt
     4261 gcaaacgatg acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa
     4321 gatacgaaaa aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat
     4381 gtaacggcgg acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt
     4441 atccgttatg acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt
     4501 gccgacacaa tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa
     4561 gaagtaaacc caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa
     4621 catacgttag aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat
     4681 ccagtgactt ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac
     4741 ttgccaaacg agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct
     4801 gtaccgatgg caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa
     4861 atgccaacag atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt
     4921 gaacaaactt cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag
     4981 agtgtggaat acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc
     5041 tatgcattag ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg
     5101 acttcattga ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca
     5161 caagatctag acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac
     5221 gatggtttaa gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc
     5281 ggtggggaaa cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt
     5341 ttggatgtcc aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta
     5401 gaaaacgatg atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca
     5461 aatccaagtg gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa
     5521 gaaaatacaa aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa
     5581 aaagaagcat atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg
     5641 ttgttccgta atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat
     5701 gatgtaccaa acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta
     5761 aaaatgaccg aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc
     5821 tctaatattc aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc
     5881 cagtttctcg gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt
     5941 ttttcttcat actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta
     6001 ttaaagcttt acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat
     6061 aaggaagtat cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa
     6121 aagaactaaa agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt
     6181 ttacccattc atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac
     6241 ttgaattttt aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat
     6301 atcctcattt gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca
     6361 gcttgtcgaa atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag
     6421 aagagaattc aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt
     6481 taggcgcgtt atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga
     6541 tttttcctaa agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac
     6601 aagaagtctt gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag
     6661 gtccagctca tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca
     6721 ctggacaagg gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag
     6781 cactgtaaaa gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt
     6841 aaatcatttg cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga
     6901 ccaaatggca gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa
     6961 tctgctaaaa gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc
     7021 cgtaagcagc tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg
     7081 ccattggagt acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag
     7141 tttttcatca ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct
     7201 ggattaggaa aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt
     7261 agtaaaccgg aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag
     7321 cagcggaaaa agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc
     7381 caagacatca tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca
     7441 gcaaaggagt ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt
     7501 gctgaaatca agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc
     7561 aatctagaat taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag
     7621 cagcgtaaag aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta
     7681 gatctttcgg aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca
     7741 aagcatacac aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag
     7801 gttaaacatt ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca
     7861 gagatccaaa aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa
     7921 aaatcaacga aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa
     7981 caagcagctg taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca
     8041 aaaagcaagc agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc
     8101 ttacaaaaag aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca
     8161 aaagagaagt tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa
     8221 gaacagccta aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa
     8281 agccttcaag aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg
     8341 cagcataaac agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct
     8401 gcagatttca cgttagctat cgaagcagct ttaggtgggg cagcccagca tgtcatcgtt
     8461 gaaaatgaaa aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga
     8521 gcaacattcc ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca
     8581 caagcagcag ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat
     8641 caaatccaaa ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact
     8701 agtgccaatg caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt
     8761 gatgtgatga atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc
     8821 agtttgtttg tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat
     8881 aaacaactcc aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta
     8941 gccgaagaac aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa
     9001 gcgacaaatc agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa
     9061 atctcaactt ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg
     9121 aacgaattga aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa
     9181 atcaaatcgt taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt
     9241 caaaaagcac aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag
     9301 atccagctcc gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata
     9361 gaaaagcagt tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt
     9421 ttggaaaaac aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca
     9481 aaagccaaag agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg
     9541 gcagagcgta atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa
     9601 aaagaccgag ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag
     9661 ctgacatttg agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga
     9721 acaaaggttt catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca
     9781 atcgaacaat acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat
     9841 ctgttagcag cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg
     9901 acaagattca aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat
     9961 atgtttggcg gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca
    10021 ggtattgaga ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct
    10081 ggaggagaac gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct
    10141 gttccgtttt gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt
    10201 tttggtcgtt acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc
    10261 aaaggaacga tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta
    10321 tcaaaaatcg tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg
    10381 aaagtgtagg aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa
    10441 atgaacaaaa gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag
    10501 gggtcaagat cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag
    10561 aattaggact ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa
    10621 aaaatgatac tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct
    10681 tatacaaact ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt
    10741 tacagctgcc tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc
    10801 aattccaacc agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa
    10861 tcgcttatga ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg
    10921 aaatgtatga gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta
    10981 caaaagctta tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca
    11041 tggcgatcgg tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag
    11101 cgatggagaa tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg
    11161 aagaaaatgg cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg
    11221 ggattttttg ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa
    11281 gtacaggaga agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg
    11341 aatgagttgt ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa
    11401 acacttatcg gtgcagatgt aggttttgaa acagcgatac gaatcaccga agcattacgt
    11461 caagaagtga aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag
    11521 aaacttgtgg atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa
    11581 gaagacggac taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt
    11641 attggtaaac ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca
    11701 gataccttcc gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt
    11761 gaagttgttc gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa
    11821 agagcaaaga cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat
    11881 aaagtgaact tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca
    11941 gaagcacccc aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt
    12001 caagcaaaac aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat
    12061 ggtacagcaa agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa
    12121 ttggtcggtc ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc
    12181 gttggattgt tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg
    12241 tgacaaaagt tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg
    12301 ccttcatttt tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac
    12361 tttttgatta taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa
    12421 ttattattgc atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg
    12481 gaaggacagc gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt
    12541 ggcgatcgat cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg
    12601 atcacccctg ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat
    12661 gtgactgctc gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc
    12721 ttaccggaaa aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg
    12781 tatcgacgtt atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat
    12841 ttcaattttg aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt
    12901 caagattttt ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc
    12961 tatcgctgga tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt
    13021 ttagatgaat cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat
    13081 acgaatcatc caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc
    13141 gagcgaatga tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt
    13201 ttccgaggcg gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg
    13261 cggaatatcg atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc
    13321 ttacaattat tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg
    13381 gtagcagaag gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca
    13441 gaatttcatc aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg
    13501 gattggttgg attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa
    13561 caaacgttag cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct
    13621 gtggcattgg gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt
    13681 tttcgcgaaa tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc
    13741 caagtcaaag tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta
    13801 gaatatgtga aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg
    13861 gaaaacaaaa cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc
    13921 gcagaatttt catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca
    13981 tttgtgatca aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga
    14041 gctagtctgg aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt
    14101 ttagtagaag aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc
    14161 caagccatca tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa
    14221 gaacttgttg aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta
    14281 gaattgatcc aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc
    14341 gaatcagttc cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca
    14401 ggtggtgatt cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt
    14461 tctgcggtag aagcactagg agctaagatt agtggtatcg atttgattat accagataaa
    14521 gaaatcgatc ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca
    14581 gccatgcata tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta
    14641 ttgaaattgt tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcc
//
LOCUS       DAZUKD010000065        14524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000065 DAZUKD010000000
VERSION     DAZUKD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.066"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..197
                     /locus_tag="ACG8N9_001745"
     CDS             <1..197
                     /locus_tag="ACG8N9_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897321.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="ACG8N9_001746"
     CDS             314..811
                     /locus_tag="ACG8N9_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4897322.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="ACG8N9_001747"
     CDS             898..1290
                     /locus_tag="ACG8N9_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4897323.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="ACG8N9_001748"
     CDS             1274..3721
                     /locus_tag="ACG8N9_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4897324.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="ACG8N9_001749"
     CDS             3724..5907
                     /locus_tag="ACG8N9_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4897325.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="ACG8N9_001750"
     CDS             5904..6905
                     /locus_tag="ACG8N9_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4897326.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="ACG8N9_001751"
     CDS             6920..7834
                     /locus_tag="ACG8N9_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4897327.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8148..8195
                     /locus_tag="ACG8N9_001752"
                     /pseudo
     CDS             8148..8195
                     /locus_tag="ACG8N9_001752"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8211..10130
                     /gene="tet(M)"
                     /locus_tag="ACG8N9_001753"
     CDS             8211..10130
                     /gene="tet(M)"
                     /locus_tag="ACG8N9_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS4897328.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10228..11601
                     /gene="tet(L)"
                     /locus_tag="ACG8N9_001754"
     CDS             10228..11601
                     /gene="tet(L)"
                     /locus_tag="ACG8N9_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS4897329.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            12165..13427
                     /gene="mobV"
                     /locus_tag="ACG8N9_001755"
     CDS             12165..13427
                     /gene="mobV"
                     /locus_tag="ACG8N9_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFS4897330.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            13672..>14524
                     /locus_tag="ACG8N9_001756"
     CDS             13672..>14524
                     /locus_tag="ACG8N9_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFS4897331.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         4743 a         2464 c         3046 g         4271 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg cgggaaaaac taccttaaca gaaagcttat
     8281 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
     8341 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
     8401 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
     8461 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
     8521 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
     8581 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
     8641 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
     8701 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
     8761 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
     8821 aaagcataag atttcagaat tgctccttgt accctgttta tcatggaagc gcaaaaagca
     8881 acatagggat tgagcagctt atagaagtga taacgaataa attttattca tcaacataca
     8941 gaaagaagtc tgaactttgc ggaaatgtct tcaaaattga atattcggaa gaaagacaac
     9001 gtcttgcata tgtacgcctt tatggcggaa tcctgcattt gcgggattcg gttagaatat
     9061 cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt gaattatgta
     9121 aaattgataa ggcttattcc ggggaaattg ttattttgca aaatgagttt ttgaagctaa
     9181 atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa aatccgctcc
     9241 ctctgctgca aacaactgtt gaaccgagca aacctcaaca aagggaaatg ttacttgatg
     9301 cacttttaga aatctccgac agtgacccgc ttctacaata ttatgtggat tctacgacac
     9361 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
     9421 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
     9481 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
     9541 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
     9601 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
     9661 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtatg
     9721 gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct cctattgtat
     9781 tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt agttttaaaa
     9841 tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa tattgtgcga
     9901 acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa atccctgctc
     9961 ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt agtgtttgtt
    10021 taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag ccccgtcgtc
    10081 caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag tgtattttat
    10141 gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa taaagatttg
    10201 aattaaagtg taaaggagga attaattatg aagtgtaatg aatgtaacag ggttaaatac
    10261 aaccaaattt taatttggct ttgcgtttta tcttttttta gcgtattaaa tgaaatggtt
    10321 ttgaacgtct cattacctga tattgcaaat gattttaata aaccacctgc gagtacaaac
    10381 tgggtgaaca cagcctttat gttaaccttt tccattggaa cagctgtata tggaaagcta
    10441 tctgatcaat taggcatcaa aaggttactc ctatttggaa ttataataaa ttgtttcggg
    10501 tcggtaattg ggtttgttgg ccattctttc ttttccttac ttattatggc tcgttttatt
    10561 caaggggctg gtgcagctgc atttccagca ctcgtaatgg ttgtagttgc gcgctatatt
    10621 ccaaaggaaa ataggggtaa agcatttggt cttattggat cgatagtagc catgggagaa
    10681 ggagtcggtc cagcgattgg tggaatgata gcccattata ttcattggtc ctatcttcta
    10741 ctcattccta tgataacaat tatcactgtt ccgtttctta tgaaattatt aaagaaagaa
    10801 gtaaggataa aaggtcattt tgatatcaaa ggaattatac taatgtctgt aggcattgta
    10861 ttttttatgt tgtttacaac atcatatagc atttcttttc ttatcgttag cgtgctgtca
    10921 ttcctgatat ttgtaaaaca tatcaagaaa gtaacagatc cttttgttga tcccggatta
    10981 gggaaaaata taccttttat gattggagtt ctttgtgggg gaattatatt tggaacagta
    11041 gcagggtttg tctctatggt tccttatatg atgaaagatg ttcaccagct aagtactgcc
    11101 gaaatcggaa gtgtaattat tttccctgga acaatgagtg tcattatttt cggctacatt
    11161 ggtgggatac ttgttgatag aagaggtcct ttatacgtgt taaacatcgg agttacattt
    11221 ctttctgtta gctttttaac tgcttccttt cttttagaaa caacatcatg gttcatgaca
    11281 attataatcg tatttgtttt aggtgggctt tcgttcacca aaacagttat atcaacaatt
    11341 gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa tgagtttgct taactttacc
    11401 agctttttat cagagggaac aggtattgca attgtaggtg gtttattatc cataccctta
    11461 cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa cttatctgta tagtaatttg
    11521 ttattacttt tttcaggaat cattgtcatt agttggctgg ttaccttgaa tgtatataaa
    11581 cattctcaaa gggatttcta aatcgttaag ggatcaactt tgggagagag ttcaaaattg
    11641 atcctttttt tataacagga attcaaatct ttttgttcca ttaaagggcg cgattgctga
    11701 ataaaagata cgagagacct ctcttgtatc ttttttattt tgagtggttt tgtccgttac
    11761 actagaaaac cgaaagacaa taaaaatttt attcttgctg agtctggctt tcggtaagct
    11821 agacaaaacg gacaaaataa aaattggcaa gggtttaaag gtggagattt tttgagtgat
    11881 cttctcaaaa aatactacct gtcccttgct gatttttaaa cgagcacgag agcaaaaccc
    11941 ccctttgctg aggtggcaga gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga
    12001 aaggtcttaa aggttttatg gttttggtcg gcactgccga cagcctcgca gagcacacac
    12061 tttatgaata taaagtataa tgtgttatac tttacttgga agtggttgcc ggaaagagcg
    12121 aaaatgcctc acatttgtgc cacctaaaaa ggagcgattt acatatgagt tatgcagttt
    12181 gtagaatgca aaaagtgaaa tcagctggac taaaaggcat gcaatttcat aatcaaagag
    12241 agcgaaaaag tagaacgaat gatgatattg accatgagcg aacacgtgaa aattatgatt
    12301 tgaaaaatga taaaaatatt gattacaacg aacgtgtcaa agaaattatt gaatcacaaa
    12361 aaacaggtac aagaaaaacg aggaaagatg ctgttcttgt aaatgagttg ctagtaacat
    12421 ctgaccgaga tttttttgag caactggatc caggagaaca aaaacgattt tttgaggaaa
    12481 gttataaatt attttccgaa cgatatggca agcaaaatat tgcttatgca acagttcata
    12541 atgatgagca aacccctcac atgcatttag gtgttgtgcc tatgcgtgat ggaaaactgc
    12601 aaggaaaaaa tgtgtttaat cgtcaagaac tgttatggct acaagataaa ttccccgagc
    12661 atatgaaaaa acagggtttt gagttgaagc gtggtgaacg tggctctgac cgtaaacata
    12721 ttgagacagc taaatttaaa aaacaaactt tggaaaaaga gattgatttt ctagaaaaaa
    12781 atttagcagt taaaaaagat gaatggactg cttatagcga taaagttaaa tcagatttag
    12841 aagtaccagc gaaacgacac atgaaaagtg ttgaagtgcc aacgggtgaa aagtccatgt
    12901 ttggtttggg aaaagaaata atgaaaacag aaaagaaacc aaccaaaaat gttgttatat
    12961 cggagcgtga ttataaaaac ttagtgactg ctgcgagaga taacgatagg ttaaaacagc
    13021 atgttagaaa tctcatgagt actgatatgg cgagagaata taaaaaatta agtaaagaac
    13081 atgggcaagt taaagaaaaa tatagtggtc ttgtagagcg atttaatgaa aatgtaaatg
    13141 attataatga gttgcttgaa gaaaacaagt ctttaaagtc taaaataagc gatttaaagc
    13201 gtgatgtgag tttaatctat gaaagcacta aggaattcct taaggaacgt acagacggct
    13261 taaaagcctt taaaaacgtt tttaaggggt ttgtagacaa ggtaaaggat aaaacagcac
    13321 aattccaaga aaaacacgat ttagaaccta aaaagaacga atttgaacta actcataacc
    13381 gagaggtaaa aaaagaacga agtcgagatc agggaatgag tttataaaat aaaaaaagca
    13441 cctgaaaagg tgtctttttt tgatggtttt gaacttgttc tttcttatct tgatatatat
    13501 agaaataacg tcatttttat tttagttgct gaaaggtgcg ttgaagtgtt ggtatgtatg
    13561 tgttttaaag tattgaaaac ccttaaaatt ggttgcacag aaaaacccca tctgttaaag
    13621 ttataagtga ccaaacaaat aactaaatag atgggggttt cttttaatat tatgtgtcct
    13681 aatagtagca tttattcaga tgaaaaatca agggttttag tggacaagac aaaaagtgga
    13741 aaagtgagac catggagaga aaagaaaatc gctaatgttg attactttga acttctgcat
    13801 attcttgaat ttaaaaaggc tgaaagagta aaagattgtg ctgaaatatt agagtataaa
    13861 caaaatcgtg aaacaggcga aagaaagttg tatcgagtgt ggttttgtaa atccaggctt
    13921 tgtccaatgt gcaactggag gagagcaatg aaacatggca ttcagtcaca aaaggttgtt
    13981 gctgaagtta ttaaacaaaa gccaacagtt cgttggttgt ttctcacatt aacagttaaa
    14041 aatgtttatg atggcgaaga attaaataag agtttgtcag atatggctca aagatttcgc
    14101 cgaatgatgc aatataaaaa aattaataaa aatcttgttg gttttatgcg tgcaacggaa
    14161 gtgacaataa ataataaaga taattcttat aatcagcaca tgcatgtatt ggtatgtgtg
    14221 gaaccaactt attttaagaa tacagaaaac tacgtgaatc aaaaacaatg gattcaattt
    14281 tggaaaaagg caatgaaatt agactatgat ccaaatgtaa aagttcaaat gattcgaccg
    14341 aaaaataaat ataaatcgga tatacaatcg gcaattgacg aaactgcaaa atatcctgta
    14401 aaggatacgg attttatgac cgatgatgaa gaaaagaatt tgaaacgttt gtctgatttg
    14461 gaggaaggtt tacaccgtaa aaggttaatc tcctatggtg gtttgttaaa agaaatacat
    14521 aaaa
//
LOCUS       DAZUKD010000066        13899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000066 DAZUKD010000000
VERSION     DAZUKD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.067"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1600
                     /locus_tag="ACG8N9_001757"
     CDS             149..1600
                     /locus_tag="ACG8N9_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4897332.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="ACG8N9_001758"
     CDS             1949..2581
                     /locus_tag="ACG8N9_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4897333.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3169..4608
                     /locus_tag="ACG8N9_001759"
     CDS             3169..4608
                     /locus_tag="ACG8N9_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4897334.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4903..5121
                     /locus_tag="ACG8N9_001760"
                     /pseudo
     CDS             4903..5121
                     /locus_tag="ACG8N9_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5370..5585
                     /locus_tag="ACG8N9_001761"
     CDS             5370..5585
                     /locus_tag="ACG8N9_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897335.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5802..6058)
                     /locus_tag="ACG8N9_001762"
                     /pseudo
     CDS             complement(5802..6058)
                     /locus_tag="ACG8N9_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6245..7156)
                     /locus_tag="ACG8N9_001763"
     CDS             complement(6245..7156)
                     /locus_tag="ACG8N9_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4897336.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7175..8179)
                     /locus_tag="ACG8N9_001764"
     CDS             complement(7175..8179)
                     /locus_tag="ACG8N9_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4897337.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8176..10299)
                     /locus_tag="ACG8N9_001765"
     CDS             complement(8176..10299)
                     /locus_tag="ACG8N9_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4897338.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10304..12751)
                     /locus_tag="ACG8N9_001766"
     CDS             complement(10304..12751)
                     /locus_tag="ACG8N9_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4897339.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12738..13127)
                     /locus_tag="ACG8N9_001767"
     CDS             complement(12738..13127)
                     /locus_tag="ACG8N9_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4897340.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13187..13690)
                     /locus_tag="ACG8N9_001768"
     CDS             complement(13187..13690)
                     /locus_tag="ACG8N9_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4897341.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13703..>13899)
                     /locus_tag="ACG8N9_001769"
     CDS             complement(13703..>13899)
                     /locus_tag="ACG8N9_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897342.1"
                     /translation="WFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLV
                     FNASGVKDVLLSLFNRIVGA"
BASE COUNT         4301 a         2728 c         2531 g         4339 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat aaacatctat
     2761 aatatattat tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa
     2821 tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg
     2881 ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga
     2941 agaacaatga agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt
     3001 tactatagag taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa
     3061 atcgaaatta ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat
     3121 aggttatatt gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat
     3181 caagaagatg caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt
     3241 accttcatgt ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat
     3301 gatatgggca taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc
     3361 tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt
     3421 cggattggta cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta
     3481 ggtattttat tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca
     3541 aatgccggaa tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca
     3601 ataggtgcgt ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg
     3661 tcccaatttt cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg
     3721 ttgattgctg aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga
     3781 gggggctttg ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg
     3841 caagaaagtg gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc
     3901 tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt
     3961 tttaagaaca agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac
     4021 ttctttgtca atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc
     4081 tatgctgggc tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc
     4141 ggctacctga ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg
     4201 tcggtaaccc aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt
     4261 gtcgctactg ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg
     4321 gttatgagct cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc
     4381 cgaaatttgg ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg
     4441 tctgctaaga tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta
     4501 tatggaatga agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg
     4561 actatctggc gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt
     4621 tgaagaattt ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg
     4681 aagtgaactt ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg
     4741 agatgcttga tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat
     4801 cccaccgatg gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat
     4861 ctgcataaga atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc
     4921 attaagatag ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat
     4981 ggtaagaagt cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat
     5041 cgaaacgtca acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt
     5101 agaaaacatc aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg
     5161 tttaggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt
     5221 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag
     5281 atgaccacta cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag
     5341 tagtctatga gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa
     5401 taaaaaaaac ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt
     5461 taagcacaat aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag
     5521 atgctataaa aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga
     5581 gataattatc gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt
     5641 gaagggggag attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga
     5701 tatctcttat taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat
     5761 aatcgtattc aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt
     5821 aggcattgct gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt
     5881 attctttatc acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa
     5941 aggcagtttt tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta
     6001 ctaaaataag ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg
     6061 tatatcaacc atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat
     6121 gtcttaattg tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg
     6181 gcgacttctg cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc
     6241 attattattt aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact
     6301 gaaagtagct agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa
     6361 aattttttcc gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat
     6421 tgattggtcg catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg
     6481 gataaagttt aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa
     6541 tttctgaatc gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca
     6601 ttgttggcaa actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat
     6661 atgcagaaga aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg
     6721 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg
     6781 aactcgtggg aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa
     6841 ggtactgttc gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa
     6901 aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct
     6961 cttttataac cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa
     7021 cgccaaaagc aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     7081 tacccacgga tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt
     7141 caatttttat cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa
     7201 gatgctgccg ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa
     7261 acatgcgatt ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg
     7321 ttgaatgaaa gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg
     7381 catcatattg atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt
     7441 gggtcaagcc actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg
     7501 ttccttcata cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag
     7561 ttgtagttag gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc
     7621 acccaccatt ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt
     7681 tttcctttga aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat
     7741 gatctagata cccgccacca tagttatagg cctgaataat actatcttga tcacacccta
     7801 gttctttgga acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct
     7861 cggtacttaa tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca
     7921 ttgtgccacc agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat
     7981 ccgattcttt ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac
     8041 tagttccgcc aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca
     8101 aacccacaca catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac
     8161 ccactttctt caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct
     8221 tttttgtact ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa
     8281 agaatttttc ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag
     8341 ttttcgtttg atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt
     8401 cagcatcata tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt
     8461 tccctgacga tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct
     8521 tcgttgtcgt tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg
     8581 cttgaattct tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa
     8641 attcgttggc gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc
     8701 ttgggtatcc aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt
     8761 attatttgaa actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa
     8821 tcgtgaattc tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt
     8881 tgccaactta tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg
     8941 attgagttgc cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc
     9001 cattgactgg ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa
     9061 atagataccg gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc
     9121 cgaaatattg aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc
     9181 cgctcgcatc ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata
     9241 agtcggcaac atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag
     9301 aatttgcgag aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt
     9361 gaacaatacc aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt
     9421 cgcattgtca ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt
     9481 ttcatctgga ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt
     9541 ggaatcatca aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc
     9601 ccgaatcaaa tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt
     9661 tccaagggta agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata
     9721 ggtaggcgca taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac
     9781 ggctcgaact gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc
     9841 cagagctaaa atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt
     9901 ttcagtaata ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat
     9961 gaaatctaat ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact
    10021 caccgtccaa atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc
    10081 atcaccccaa ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata
    10141 gttgttcagt gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc
    10201 tgtcgcctcg gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat
    10261 aaaaacgatt cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt
    10321 gaacaggtgg acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa
    10381 ccacgcctac ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta
    10441 ggcgtttttg attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga
    10501 tttcatgaat atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt
    10561 catccagtaa atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc
    10621 cagcacgaac gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg
    10681 cctcatctaa gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa
    10741 aagtggaaat gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa
    10801 aagtggtttc tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat
    10861 ctaaactaat cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat
    10921 cggtaaagga ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa
    10981 gctcattaat tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct
    11041 tacgcaacac tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa
    11101 tatcaattgc tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta
    11161 atccttgatt ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg
    11221 gcaaggtttc tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac
    11281 caaaaaggac ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg
    11341 aaccgaggaa tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca
    11401 atgccggttt caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt
    11461 ccaactcccc taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa
    11521 catactggat ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc
    11581 ccatcatatc gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca
    11641 cttcatcaca ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc
    11701 tcagtttgta catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat
    11761 ccaatgcatc taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca
    11821 agtcttttaa ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa
    11881 tctccacatt catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa
    11941 gttctgagga gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat
    12001 atgattcatg attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac
    12061 gactgggacg taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg
    12121 gtagctgaaa ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca
    12181 aatacatcag atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt
    12241 ccattagttt ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat
    12301 cgcccatcaa atgatgattc actccataga caaattcttg aaagccggag aagaaagatt
    12361 tcttgagatt ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa
    12421 agcgataatc aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa
    12481 ttcgttgttt cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg
    12541 tggcacgaat actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt
    12601 gcgcaatcaa ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg
    12661 agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa
    12721 gattattttc aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt
    12781 tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt
    12841 aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt
    12901 tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct
    12961 atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac
    13021 caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg
    13081 accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt
    13141 taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact
    13201 caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg
    13261 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta
    13321 caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat
    13381 ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa
    13441 cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct
    13501 caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct
    13561 cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa
    13621 accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca
    13681 tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga
    13741 catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa
    13801 agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg
    13861 ccagtaagac taaggattgg gcattggtta aaaaccatt
//
LOCUS       DAZUKD010000067        13842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000067 DAZUKD010000000
VERSION     DAZUKD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.068"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..460
                     /gene="yaaA"
                     /locus_tag="ACG8N9_001770"
     CDS             218..460
                     /gene="yaaA"
                     /locus_tag="ACG8N9_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4897343.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            447..1571
                     /gene="recF"
                     /locus_tag="ACG8N9_001771"
     CDS             447..1571
                     /gene="recF"
                     /locus_tag="ACG8N9_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4897344.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1568..3514
                     /gene="gyrB"
                     /locus_tag="ACG8N9_001772"
     CDS             1568..3514
                     /gene="gyrB"
                     /locus_tag="ACG8N9_001772"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4897345.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3534..6005
                     /gene="gyrA"
                     /locus_tag="ACG8N9_001773"
     CDS             3534..6005
                     /gene="gyrA"
                     /locus_tag="ACG8N9_001773"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4897346.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6241..6540
                     /gene="rpsF"
                     /locus_tag="ACG8N9_001774"
     CDS             6241..6540
                     /gene="rpsF"
                     /locus_tag="ACG8N9_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4897347.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6590..7102
                     /gene="ssb"
                     /locus_tag="ACG8N9_001775"
     CDS             6590..7102
                     /gene="ssb"
                     /locus_tag="ACG8N9_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4897348.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7128..7364
                     /gene="rpsR"
                     /locus_tag="ACG8N9_001776"
     CDS             7128..7364
                     /gene="rpsR"
                     /locus_tag="ACG8N9_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4897349.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7507..9480
                     /locus_tag="ACG8N9_001777"
     CDS             7507..9480
                     /locus_tag="ACG8N9_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4897350.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9486..9938
                     /gene="rplI"
                     /locus_tag="ACG8N9_001778"
     CDS             9486..9938
                     /gene="rplI"
                     /locus_tag="ACG8N9_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4897351.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10212..11579
                     /gene="dnaB"
                     /locus_tag="ACG8N9_001779"
     CDS             10212..11579
                     /gene="dnaB"
                     /locus_tag="ACG8N9_001779"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4897352.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12359..13651
                     /locus_tag="ACG8N9_001780"
     CDS             12359..13651
                     /locus_tag="ACG8N9_001780"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4897353.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4622 a         2491 c         3032 g         3697 t
ORIGIN      
        1 aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa acagaaggcc
       61 ctaaaatctc ttttaaggct ttctgtttat ttttgtttaa atcacctttt ttagattaaa
      121 tcgtcttata tggaaaaata tatggataaa tttgttttat tgcgaaaaaa aaggtataat
      181 agagtatact tttaataatg aaaatgaggg atgggcattg aaacaacaag tcattctaaa
      241 tacggaattc atgacgttgg gccaaatgtt gaaagaagtc acagttatcg gtagtggcgg
      301 acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc gatggcgaac cagaaaatcg
      361 tcgtggacgc aaactttatc ccggcatgat ggttgaagtt ccagaagtag gaactttttt
      421 tatggtaaaa aaaggaaacg acgataatga ggctgaataa gctgtattta aaaaattatc
      481 gaaattacga agagctgaac atcgaatttt ccaagaacct cgtcatcttt ttaggagaaa
      541 atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt attagctatg acgcgaagtc
      601 atcggacaag tagtgagcag gaattgattg gctggactga cgatcaggcg atgattcaag
      661 gagagatcac aaaaggttca tccactcttc cattagaaat cttattatct aaaaaaggcc
      721 gaaaaaccaa gatcaaccat atcgaacaga aaaagttaag tacttacgtg ggacaaatga
      781 atgtcatttt atttgcacca gaagatcttt ctttagtaaa aggcagcccg cagatcaggc
      841 ggaagttttt agatatggaa ttaggccaga tcaatccaat ctatctttat gatcttgtcc
      901 aataccaagc aattttgaaa caaagaaacc agtacttgaa gcaattgaat gaaaaaaaac
      961 aaactgattt gctttatttg gatattttgt ccgaacaact agcagcgttt ggcagcaaag
     1021 tattgaaagc aagagcacag ttcgtcaagc gattggagtt ttgggctaat tctttgcatc
     1081 agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca gtcgattctt
     1141 tagaaacaca tacgcaagag caaatccaag aacaatttct cgcacttttg aaccaaaata
     1201 aaaagaaaga cctttttcgg ggaacaacga cagttggtcc tcatcgtgac gatctttcct
     1261 tttttattaa tcagaaaaat gtgcagactt atggatcgca aggacagcaa agaacaactg
     1321 cactaagtgt caaattagca gaaatcgatt tgattaaaga agagacaggg gaatatccaa
     1381 tccttttgtt agatgatgtg atgagcgagc tggatgataa tcgtcaactc catctgctag
     1441 aaacaatcga aggaaaagtt caaacgttct taacaacaac gacacttgac catgtaaaaa
     1501 ataaaatgac tgttgaacct gagatttttt atgttcaaca agggaaacta aaaggagaag
     1561 aaactgaatg acagaagaaa gaagtttagt agaacgcgct aaagagtatg atgccagtca
     1621 gattcaagta cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt atatcggttc
     1681 gactagttct gagggtctgc atcaccttgt ttgggaaatt gtcgataatt cgatcgatga
     1741 agtattagca ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca acagtatcac
     1801 cgttattgac gacggtcggg ggatacctgt agatatccag gcaaaaactg gacgtcctgc
     1861 agttgaaacg gtctttactg ttttacatgc tggaggaaaa ttcggcggtg gtgggtacaa
     1921 agtttctggt ggacttcacg gagtaggatc ttctgttgta aacgcgcttt ctacacagct
     1981 tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc gtggagctgt
     2041 agtggacgac ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg ttcactttac
     2101 gccagaccct gaaattttta cagagacaac tgagtttgat ttttcaaaat tggctacccg
     2161 gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata tcgattgaag acaaacgaga
     2221 agaagaacca gtcatcaatg aataccatta tgacggcggg atcaaaagtt atgtcgaata
     2281 cttgaatgca aacaaaaccg tgatttttcc agaacctgta tatttggaag gtgaacagca
     2341 agatatcgca gttgaagtct ctatgcaata tacagacggc taccattcca atatcatgag
     2401 ttttgccaac aatatccata cgtatgaagg tggaacacat gaatcaggat tcaagactgc
     2461 tttaactcgt gtgataaatg attatgcccg aaaacaaaag ctgatgaaag aaaatgacga
     2521 caacttgacc ggtgaagacg tgcgagaagg attgacagct gttatttcta tcaagcatcc
     2581 tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc aattccgaag tacggacagt
     2641 cactgatcgg ttatttagcg aacatttcat gaaattcttg ctggaaaacc caagtgtggg
     2701 ccggcaaatt gttgaaaaag gattactagc ttccagagct cgtctagcag cgaaacgtgc
     2761 gcgtgaagta acgagacgta aaggtgcttt agagattagt aatctgccag gaaaattagc
     2821 ggactgctct agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag gagattcggc
     2881 cggcggatca gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc ctattcgtgg
     2941 gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg aagagatccg
     3001 gtctttattt actgctatgg gtacagggtt tggagaagat tttgatgtat cgaaagctcg
     3061 ctaccacaaa ttagttatca tgactgatgc cgatgtcgac ggcgcgcata tccgtacatt
     3121 gctgctgact cttttttatc gttacatgcg tcctattgtt gaagctggat acgtctatat
     3181 tgctcagcca cctttatacg gggtaaaaca agggaagaat attacgtatg tacaaccagg
     3241 aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca cttcctgcct caccaaaacc
     3301 aagcgttcag cgttataaag gacttggaga aatggatgat catcaactat gggaaacaac
     3361 tatggaccca agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta ttgcagcaga
     3421 tcagatattt gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat tcatcgagga
     3481 aaatgcacac tatgtgaaga acttagatat ttaatcatga aggaggaaaa agaatgagtg
     3541 aagaaatcag agaaaacatc catgatgtca atctaaccag cgaaatgaaa gactccttca
     3601 tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga gatggtctga
     3661 aacccgttca tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca ccggataaag
     3721 cccataaaaa atctgccaga atcgtcggag atgtcatggg taaataccat cctcatggag
     3781 acattgcgat ctatgagtca atggtacgga tggcacagcc attcagctac cgttatatgc
     3841 tagtggatgg tcatggaaac tttggttctg ttgacggtga cggtgccgcc gctatgcgtt
     3901 ataccgaagc acggatgagc aaaatcgcta cagaaatgct gcgtgatatc aataagaaca
     3961 ctgttgattt ccaaagcaac tatgatgata cagaaaaaga accagttgta ctacctgctc
     4021 gcttcccgaa tcttttagtg aacggaacaa cggggatcgc cgtaggtatg gccacgaata
     4081 tcccacctca taacttgtct gaggtagtag cggctatcga tttgctgatg gataatcctg
     4141 atgtgacgac caatgaattg atggaagtat tacctggacc agattttcca acaggtggtt
     4201 tggttatggg gaaatcagga atccgaagag cgtatgaaac aggtaagggt tctattaccg
     4261 tacgtgcaaa agttgagatt actgagatgc caaatggtaa agaacggatc atcgtgacag
     4321 aattgcctta tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa ctgcacagag
     4381 acaagcgtat cgaagggatc acagatttgc gggacgaatc ttcccgtgaa ggtatgcgga
     4441 tcgtcattga catccgtcgt gacgccagcg catccgttat cttgaataac ttatataaat
     4501 tgacatcctt gcaaaattca tttggattca atatgttggc gatcgaaaaa ggtgtgccga
     4561 aagttttgag cttgaaacaa atccttgaaa actatatcga acatcaacgc gaagtcatca
     4621 cacgacggac ggtctttgaa aaagaaaaag cagaagcaag agcacacatc ttagaaggtt
     4681 tgcgtattgc tttggatcat atcgatgaaa tcattgcgat tatccgcggg tctaaatcag
     4741 atgacgaggc aaaagcaaca atgatcgaac ggttcgattt atccgatcgc cagtctcaag
     4801 ctattttaga tatgcgttta cgccgattga ccggtttgga acgggaaaaa atcgaaaatg
     4861 aataccaaga actcttaaaa ctgattgaag atttaactga cattctggca agaccagaac
     4921 gtgttacaga aatcatcaag aatgaattag cagacttgaa tcaacgattt ggtgataaac
     4981 gacgcacaga attactagtc ggtgaggtct taagtctgga agatgaagat ttaatcgaag
     5041 aagaagaaat cgttattaca ttgacaaaca atggttacat caaacgtttg gcaaataatg
     5101 aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc cacaatgacg
     5161 acttcgtcaa aaatctggtt tcttgttcta cccatgatac attgttattc tttacgaata
     5221 acggaaaagt gtatcgggca aaaggatacg aaattccaga atacgggcgt acagccaaag
     5281 gaatcccaat catcaattta ctaggtatcg attcgagtga aagcatccag gcaatcattg
     5341 cggtgacggg agaagcagaa gaaggccact atctcttctt tacgacgcgt caaggtacag
     5401 taaaacgtac ttctgtcact gcttttgcta acatccgaag taatggtctg atcgcaattg
     5461 gtttgaaaga agacgatgag ctcgtcaatg tgctactgac agatggtcaa tctaatatca
     5521 ttattggaac acataacggc tactctgtca ctttcaaaga agaagcagtc cgcgatatgg
     5581 gacgtacagc ttccggggtc agagggatac gcctgcgtga ggaagattat gtcgttgggg
     5641 cagccttaat gaaggaaaat caagaagtat tagtgatcac agaaaaaggt tacggaaaac
     5701 ggacaaaagt atcggaatat ccagtgaaag gccgcggagg taaagggatc aaaacggcga
     5761 acatcactga aaaaaatggt ccactagctg gattgaccac tgtttctgga gatgaagaca
     5821 tcatgctgat tacagataaa ggtgtcatta tccgtttcaa cgtatcgact gtttctcaaa
     5881 ctggacgttc aacattaggc gtacgtttga tgaagatgga agctgatacg aaagttgtca
     5941 cgatggcagc agtcgaacca gaagctgcag aacaaacagt tgaagaacaa ggatcagaag
     6001 aataaccatc aagactaagt gtgtaagcta gcaagaataa ccgattaaaa aatctggtta
     6061 ttcctgctgg cttttttgat tagttgaatt tctcttaaaa tattcgaaac tgttcattct
     6121 gtattgcttt tgaccaaaat agagtgtata attagagctt gtgagtagtc taattaatta
     6181 gataactctc cttgctctta ggaacctaag agccgaaagt ccacagggag gtgaatatca
     6241 atggaaaaca cgaaatatga aatcatgtac attattcgtc caaacattga tgaagaagca
     6301 aaaactgctt taatcgaacg tttcgatgca atcttgaaag ataacggagc agaagttatc
     6361 gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa tgaacggtta ccgtgaaggt
     6421 atctatcaca tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa tgaatttgac
     6481 cgtcttgcta aaatcaacga cgatatcatt cgtcacatga ttgttaaaga agaagcttaa
     6541 ttgttgtttc atgtgaaaca atagaaaacg atgagaggag atgactattt tgattaataa
     6601 tgttgtatta gttggacgtc tgacgaaaga tccagatttg cgttacacag caagtggtac
     6661 ggcagtcgca acatttactt tggctgtgaa tcgtaatttt acaaaccaaa gcggcaatcg
     6721 cgaggctgat tttatcaact gtgtgatttg gcgcaaatca gcagaaactt tagcaaacta
     6781 tgctcgtaaa ggaacacttt taggtgttac tggacgtatc caaactcgtt cttatgataa
     6841 tcagcaagga caacgtgtat acgtgacaga agtagtagct gacaacttcc aattgctgga
     6901 aagtcgttct gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt ctggtcaatc
     6961 agctggcgga tttggcggaa acaataactc taatcaaaca tctcaatcat ccaacggtat
     7021 gcctgatttc gaccgtgata cttctgaccc attcggctca agttcaacga tcgacatttc
     7081 agacgatgat ttaccattct aaacgaattt gataggaggg aaatagaatg gcacaacaaa
     7141 gaagaggcgg acgtaaacgt cgtaaagtag actacattgc tgctaaccat attgaatata
     7201 tcgattataa agatatcgaa ttactaaaaa gatttatttc agaacgcggt aaaatcttgc
     7261 cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa aattactgtt gcaatcaaac
     7321 gcgctcgtat catgggcttg ctacctttcg ttagcgaaga ctaataaatc ctaggaattg
     7381 aatgactgat ccttttgatc agctattcaa ttcctttttt tctgtaaaaa taacctctat
     7441 tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca aattagtcat atatgggagg
     7501 aaaattatgg aaaataaaaa caatcattta tccccgaaac ctctcttcct tatcctgttt
     7561 atattaatcg aagtattggt agtcttaatt ttaccatttc gttttgttag cgcttcggtc
     7621 attctattga tgaatttatg gggtgttcgc tattggttga aaatgaaacg ttggcttatt
     7681 ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa tctagcttat
     7741 gtctcagaag tcatgcctgt aggagtgatt gcttatcagc catcaagcca aaaaatcgaa
     7801 tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg gattaacaga ggaagaattg
     7861 ctagatactt tttttactgc aaaagaaaaa aagaaaaacc aagtctatct agcagataaa
     7921 acatttcagt ttgatatgga tacagtaaag ggtgttgttt attttaatga tattacccaa
     7981 aaccagttgt taaaactcca tcagacgaac agtcagccgg tgattggtat tttatctatt
     8041 gataactatg cagatgccat tgataaatta gatgacaaag agatttctta tttgaactct
     8101 ttcgtcacta cactagtttc tgactggatg aatgaatacc atgtcttttt caaaagaatc
     8161 aacgcagaac ggtttttctt cgtagcacag atgatcgatc tgaaaaagat gatggataaa
     8221 aaatttgatt tgctggatcg ctttcgagca gaagctgaaa aacagagctc gcctatcacc
     8281 cttagtatgg ggattgctta tggctcaaga accctcgatg aaatcggtgg agaagctcag
     8341 aataatttag atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa agaggtaaaa
     8401 gaaggagcaa aacctgttta ttatgggggg aaatctgcta agaatgtcaa acgcacgcgt
     8461 gttcgttctc gagcgatgag taccgctttg cgtcggattt ttgatgaaac aggcgatgtg
     8521 tttatcatgg ggcacaagtt tccggatatg gatgctttag gctctgcttt tggtgtcagt
     8581 tgtctggcac gcttcaacca gaaaaaagct tacatcgtta tcaatgagaa cgagatcatt
     8641 ccagatgtag aacggtgtct tgaagaaatc cataagcatc ctgaattaga aatgcaacta
     8701 atctctccag aacgtgccat agagctgaaa aaagaagagg acctcctcgt gatggtggat
     8761 taccataaac cttctctatc gatctctcag ggcctatatg agcaatttga taagattgtg
     8821 atcattgatc accatcgtcg tggggaagag tttccaagta aacctctgct gacttatatt
     8881 gaatcttcag cttcttcagc ttcagagcta gtttctgagc tgatccaata ccaaagcagc
     8941 aaaacaaatc gcttagatcg ttttgaggcc acattattgc ttgcagggat ggttgtagat
     9001 acaaatagtt tcaatgtccg tacaactgca cgaacttttg acgttgccag ctatttaagg
     9061 tcttgtggag cagacgcatc cttagtacaa tatttattga gctcggattt agcgacatac
     9121 ttggagatca gcaatttgat tgctggaagc gactatgtga cttctgatat tgttgtggcg
     9181 gcagggacag acgaaaaaga gtataatagt gttatagcag caaaaacagc agatacattg
     9241 ctttcgatga tcaatatcaa tgcagctttc gtcattacaa agcggacaga cggcttgatt
     9301 gggatcagtg ccagaagtac cggcacgatc aacgtccaaa tcatcatgga atccttaggt
     9361 ggcggaggac acttcacaaa cgcagctact caactggaaa atacgacagt tgaggaaaca
     9421 aaacagaagc tatttgctgc aatcaaagaa aatatgaatc aaatatatga taaggagtaa
     9481 tccaaatgaa agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa ggggacatca
     9541 aagaagttcc agcaggttat gcacaaaatt atctaatcaa aaataacttg gcaaaagaag
     9601 caacgaaaag cagtatcagt cagttagcag gacaaaagaa agcacaagaa aaacacgaag
     9661 ctgaaatctt ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa gatactgtag
     9721 tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc ccttcaaaac
     9781 aaattgctga agggttaaac aaacaataca aaatcaaatt agataaacga aaaatcgaat
     9841 tagaaaatcc gatccgttca ctaggctaca caaacgtacc tgttaagctt catcatgagg
     9901 tgacggctac gatcaaagtt catgtcgtac aagaataaca aatcttgata gcgatgttca
     9961 gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag aaaacagctg
    10021 cagcaatgaa agcaaaagct gcaaatcgat ttcagattga tttcggtttc ttttttggga
    10081 acagcaaaat agcataaaga aaacgagtat aataaaagag aactattttt ttattacact
    10141 ttcggtacga atgatgactt ttctctcatt ttcttgtaaa atgaagcaat tgaagacaag
    10201 gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat attgaagctg
    10261 aacaagcggt tttaggctct gtttttcttg acgcagaaac gatcattgat gccatggagt
    10321 acatagagcc aaaagacttt tatcgtcgtg gccatcaaat tatttttgag acaatgatcg
    10381 agttaaatga tcgtaatgaa gcgattgacg tggttactgt caaagaccgg ctggaacaag
    10441 ccaatttgtt agaagatgcc ggtggtttaa gttatctctc agacttggca ttagcagttc
    10501 cgacagcagc aaatatcgtc tactatgcga aaatcgttga agaaaaatca ttgctgcgta
    10561 cattgatcca gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga gaagaagttc
    10621 aaacaatttt agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa agaaatcgaa
    10681 gcggcttttt atctattgct gaagtactga actccacgat tgaaaatatc gatcgactag
    10741 ctcaaaacaa tgaagaaatc acaggtctac caactggcta tcaaggtctg gataaaatga
    10801 cagcaggttt ccagcccgaa gaactaatca ttcttgctgc acgtccagcg gtaggtaaaa
    10861 cagcgtttgc attgaatatt gctcaaaatg taggaacaaa aacagatcgt tctgttgcga
    10921 tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt gcagaaggtt
    10981 cgattgaggc cagccattta cgtacaggtc agctttcgga agaagaatgg tcgaatctga
    11041 tcgttgcgat ggggagtctt tctcgagcaa atatttttat cgatgataca ccagggatca
    11101 aaatctctga aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc aatctaggct
    11161 tgatattgat cgattatttg cagttgattg aaggtaacgg taaagagaac cgtcaacaag
    11221 aagtatcaga tatctctcgc caactgaaaa aactggcgaa agagctgaaa gtacctgtca
    11281 ttgctctttc acagttatct cgtggagtag aacagcgaca agacaaacga ccagtgctaa
    11341 gtgatattcg tgaatccgga tcaattgaac aagatgcgga catcgtagcc ttcttgtatc
    11401 gagatgatta ttatgaaaga gaaggcggag atgacgatga tggaaacgag ccaaaaaatg
    11461 acaacgtcat cgaagtcatc atcgagaaga accgaagcgg tgcacgagga acagttgaac
    11521 tactgtttat caaagaatac aataagtttt cgtcgctttc gccaagagaa gaattttaaa
    11581 gaagaggttg tgagccttga gcaatcccga gagctctcgt aagaagatgc aaggagtaac
    11641 cgcaggaacg actagagtac ttaatttaaa cagatctcca aagggattgt gacaaaagtc
    11701 ttgtcacagt ccctttttcc gaataaacgg tgttcaatca gctgaccctt ggtattagct
    11761 cagcaaacgt caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
    11821 aacgctgatc ttacgacctc ttttccttaa acggtgttct cctgccaact cctcggtcac
    11881 aagtcacaat atcatcaaac atgagaagca gttttttgaa attgctcctt gtgactcaga
    11941 gttaaacggc aggctcacaa cctctttctt aaacggtgtt caaaagcttg catctgcggt
    12001 aatacgccca atcaaacgaa aaatatgaga agctattttt gtttggctgt tcttattact
    12061 tgatgcagga cagctttttc acaacctctg attcacggtg ttccaaaagt agcttcttcg
    12121 aaaaataagt cgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttatt
    12181 tcttgaagct gaccacttct gttacaacct cttttttaat aaccattcta aaaagataaa
    12241 gttcgttttt taagcgtttt tataaaataa aaataaaaat cattcgattt tatttgaaat
    12301 atgtatctat tattgataca atggttcagg attaaataat atagaacgag gtgttcacat
    12361 gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa aaatcacaga
    12421 ctttctcagt gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca atgcagggca
    12481 cacaattaag tttaacggtg tgacttataa actgcatttg attccttctg ggatttttta
    12541 taaagacaag atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat cattggttaa
    12601 agaattagct tatttacatg aaaaaggtgt agaaacaagt aatttgcgta tttctgaccg
    12661 cgcgcacgta atcttgcctt atcatatcaa attggaccaa ttacaagaag atgctaaggg
    12721 cgaaaataag attggtacaa cgattaaagg aatcggacct gcctatatgg ataaagcagc
    12781 tcgtgtaggt atccgtattg cagacttatt agataaagaa atttttgaag aacgtcttcg
    12841 tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag aaccacttga
    12901 ttttgacgat atttttgaag aatattatga atacggacaa caaatcaaac aatacgtcac
    12961 agatacttcc gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt tgtttgaagg
    13021 cgcacaaggt gtaatgctgg atatcgacca gggaacttat ccttttgtta cttcttctaa
    13081 cccagtagcc ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa tcaataaagt
    13141 tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc caactgaatt
    13201 atttgatgaa acaggagaac aaatccgcga agtaggacga gaatacggta cgacaactgg
    13261 tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg cgccattcta aacgagtttc
    13321 aggtattacc aacctatctt taaactcgat cgatgtatta agtggattgg aaacagtaaa
    13381 aatttgtacg gcttatgaat tagatggcga actaatctac cactatccag caagcttgaa
    13441 agagttgaac cgctgcaaac caatctatga agaattgcca ggctggtcag aagacattac
    13501 aggatgcaaa acacttgcag aactgccaga aaacgcacgt aactatgtac gtcgtatctc
    13561 agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa cacaaacaaa
    13621 catcttagaa agcgtctggg cgcaaatcta aggataaaaa cgacgactga atcaggacgt
    13681 tcttaccaga gcgagtcgaa gtgtttatct atacgataat aataacttga agaggtagtg
    13741 aaagagacgt ttagccttat tgccgatagg ctgtctcttg aacactgttt attcagaata
    13801 agggactgtg acaaaaaccg tgtcacagtc cctttttccg aa
//
LOCUS       DAZUKD010000068        13810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000068 DAZUKD010000000
VERSION     DAZUKD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.069"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG8N9_001781"
     CDS             complement(221..1423)
                     /locus_tag="ACG8N9_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4897354.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG8N9_001782"
     CDS             complement(1426..1620)
                     /locus_tag="ACG8N9_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4897355.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG8N9_001783"
     CDS             complement(1667..2515)
                     /locus_tag="ACG8N9_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS4897356.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG8N9_001784"
     CDS             complement(2720..3070)
                     /locus_tag="ACG8N9_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897357.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG8N9_001785"
     CDS             3248..4111
                     /locus_tag="ACG8N9_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4897358.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG8N9_001786"
     CDS             4735..5727
                     /locus_tag="ACG8N9_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897359.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG8N9_001787"
     CDS             5743..6627
                     /locus_tag="ACG8N9_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897360.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG8N9_001788"
     CDS             6676..8283
                     /locus_tag="ACG8N9_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4897361.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG8N9_001789"
     CDS             8284..9768
                     /locus_tag="ACG8N9_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4897362.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG8N9_001790"
     CDS             9945..10937
                     /locus_tag="ACG8N9_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4897363.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG8N9_001791"
     CDS             10989..11858
                     /locus_tag="ACG8N9_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4897364.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8N9_001792"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8N9_001792"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4897365.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZUKD010000069        13801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000069 DAZUKD010000000
VERSION     DAZUKD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.070"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..313)
                     /locus_tag="ACG8N9_001793"
     CDS             complement(<1..313)
                     /locus_tag="ACG8N9_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897366.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITVAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LA"
     gene            complement(461..1003)
                     /locus_tag="ACG8N9_001794"
     CDS             complement(461..1003)
                     /locus_tag="ACG8N9_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4897367.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1138..1527
                     /locus_tag="ACG8N9_001795"
     CDS             1138..1527
                     /locus_tag="ACG8N9_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897368.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1531..2295
                     /locus_tag="ACG8N9_001796"
     CDS             1531..2295
                     /locus_tag="ACG8N9_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4897369.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2424..3371
                     /locus_tag="ACG8N9_001797"
     CDS             2424..3371
                     /locus_tag="ACG8N9_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4897370.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3501..4241
                     /locus_tag="ACG8N9_001798"
     CDS             3501..4241
                     /locus_tag="ACG8N9_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897371.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4532..5200)
                     /locus_tag="ACG8N9_001799"
     CDS             complement(4532..5200)
                     /locus_tag="ACG8N9_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4897372.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5334..5621
                     /locus_tag="ACG8N9_001800"
     CDS             5334..5621
                     /locus_tag="ACG8N9_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897373.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6037..7575
                     /locus_tag="ACG8N9_001801"
     CDS             6037..7575
                     /locus_tag="ACG8N9_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4897374.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7572..8537
                     /locus_tag="ACG8N9_001802"
     CDS             7572..8537
                     /locus_tag="ACG8N9_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897375.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8524..9846
                     /locus_tag="ACG8N9_001803"
     CDS             8524..9846
                     /locus_tag="ACG8N9_001803"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4897376.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9909..10013
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10087..10830
                     /locus_tag="ACG8N9_001804"
     CDS             10087..10830
                     /locus_tag="ACG8N9_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897377.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10845..11837
                     /locus_tag="ACG8N9_001805"
     CDS             10845..11837
                     /locus_tag="ACG8N9_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4897378.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVNYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11849..12586
                     /locus_tag="ACG8N9_001806"
     CDS             11849..12586
                     /locus_tag="ACG8N9_001806"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897379.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12576..13298
                     /locus_tag="ACG8N9_001807"
     CDS             12576..13298
                     /locus_tag="ACG8N9_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4897380.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13382..>13801)
                     /locus_tag="ACG8N9_001808"
     CDS             complement(13382..>13801)
                     /locus_tag="ACG8N9_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4897381.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4589 a         2350 c         2853 g         4009 t
ORIGIN      
        1 ttgctaaaaa agaaaacgtt ttaaatgcaa tcaatacgct tgaagcttcg tttaacgtac
       61 caggatgtcg atgaaaaaaa taggcaccga aataaacaga aaacagtaag ccgcaaagag
      121 aagacaaaat caaaaaaaac ggattttctt cggctactgt gatatcagaa tgatccaaaa
      181 gcaaccagcc taatgcacta gaaggcgcat gtgaggtctt aaggtatgaa taacatagaa
      241 aaaataagat cgtcaaagtt gcaaaaaaag agcctaaatg taaaaaatta gtggttctac
      301 gttgaatttc cataatatct ccaaattaaa ttgataagta tataaagtat aataacaaat
      361 tgatgagtat atgtcgatgt tttttaatca aaataataaa taaaaactgt ctaacattta
      421 aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct tgtcgttttc
      481 tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa atgataaaag
      541 cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt ctaggagaaa
      601 gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt gtcacaccat
      661 aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt gctggcaaaa
      721 tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt ccgacactga
      781 tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct aaacgatcta
      841 aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca agtggagcaa
      901 tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg atataagcaa
      961 aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa actcctttca
     1021 tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa ctttatacat
     1081 acaaaaaaat gataaaatga ttataataga attataaaga taggttaggt gattaaaatg
     1141 aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct cagtatatac
     1201 ttaggtgtct attcgattca acagccaatt gaggtagaga acattgtttt tcagatgtcg
     1261 ctgacagatg gtttaatttt aagcgttttt ttctttttga tactgtttat tggtaatatt
     1321 ttcggtagcg ttgccagttt tttgaatttt tctatttatc cactcctttc gctagcatta
     1381 ggagtggtgg gactttgttc tctgtgctta tttcccgagc cattcagtcc tgcatttata
     1441 ttatttggta cactgaacct gttccagaca actgttggag catggttgct atggcgatca
     1501 ggaaatttaa tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa acgttctgtc
     1561 catcatattc cagtgttaga agttgttctg aaagaattaa gagaggaacg tattcccgtt
     1621 gtcatctatt atcacggatg gcagacaagt aaggagttag ttttgacgca aggaagaaaa
     1681 attgcgcagc agggcattcg tgtgatcctg ccagatgcga tgaatcatgg ggagcgcaaa
     1741 cagccggttt cttctattcc atcctataca ttttgggata gtatctacgg aaatctgttt
     1801 gaatttgaca cacttatcaa gcatttagaa aaaagaggtc ttctacatga tcagcttgca
     1861 gttggcgggg tttcgatggg gggaatgaca acatgcgcct tgttggctaa acatcctcat
     1921 atagtagcgg gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg aaaagaaatc
     1981 gtaaggcgcg catcggaata taattatgtc gtaccaaaag actattttga tttgatttca
     2041 tgggttaaga gctatgattt gtcattgcag ccggaaaagt tagctggacg tcctctgttc
     2101 ttttggcatg gacaagctga cgaaaaaatc ccttatatac aaacggaacg atttgtagaa
     2161 aaaaatcggc ataagtctta cggaaaagaa attgtttttc gttcctcaaa aacacagcga
     2221 catatggtac aaataccact aatggaagaa gcttccactt ttttagcaaa aaatttgata
     2281 aaaaagtata aataattgaa acggaaaatc actcatgacg atagaggatt ttccgttttt
     2341 tattttgggc tataataaac tctgttagga aaatttggta taattgatag taagaatgac
     2401 aacatgttag aaaggaagag agcatgccca ctttagcaga tgttgcaaaa cgtgccaatg
     2461 tttcaaaaat gacggtctct cgagtcatca atcatccgga acaggtgact gacgaattga
     2521 aagagttagt tttctcagca atggctgaac tggattaccg tccaaatatt gctgcaaaag
     2581 cacttgtcac gaatcggtcg cagatcatca aattatttat actggaagaa atcgacacaa
     2641 ctgagcccta ttacatgaat ttgctgatgg gaatcgctaa acggatcggt aaagcgcatt
     2701 attctctcca attagttaca aatgatgcct tcgacgtcgg ttcttgtgat ggatacatca
     2761 tcacaggtgt cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa ccagttgtct
     2821 tgtttgggga aaattctgaa ggatatgatt atgtagacag cgataatgaa tatggtacag
     2881 caaaagcaac gcaatatgcc ttagaaagag attatcaaac gattatttat atcggtatcg
     2941 aagtggacga gccttttgaa atttcaaggg aaactggtta tttatctgtg atgaaggaag
     3001 ttgatcttcc tagtaaaatc ttgcggtttc caaatcattc gacagaagta gaaagatata
     3061 tgacagaaaa ctgggcagat tttacagata aaacttgttt tgtatgtagt tcagatcggc
     3121 tagcactagg agcagtaaga gggattataa atgctggagg acacttgccc gaagaatttg
     3181 gtgtgattgg atttgatggg gtatttttag accaaatcag ttcacctaaa ttgacaacga
     3241 tcaagcaaga tattatacgt atgggtgaag tatgtggaga aatgttgtta aaaaaaatag
     3301 aagaaaaagg ggcgagtcaa ggttatcgtc gtttttttcc tgaactagtt atcagagaga
     3361 caacgaatta agtcttatta ctatatatat atttatgaaa ttcatcgcta ttttcttaaa
     3421 ataatcagcg cattgctact gaaaacaatg aatctgtgct attattgaga aaagttccat
     3481 ttttgagaag gatggtttga atgaaagaaa tcagacgaaa acatttccgt tttcctgcgt
     3541 acgatgatga gttaggcgta aaattaacca acgacaagaa gcgagtatta tttgacggac
     3601 aagaagatct tttgactgaa cagaatcagt ccccgtttga acagatgaaa gaatttcatt
     3661 tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat ggattgaagt
     3721 cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa attaccggat tataccggcc
     3781 attaccaatc agaaatcaca tcgacaggac gaaaaaaact ttttggtgat tcctctcgtt
     3841 ctgtcagaca aagtgaaaca aggacagagt ccaagcatac tacacgaaac gcatctgttg
     3901 acacgaaggt gcgggagcgt tcttattttg ttcctaaata tgtacccgca tcgattatcc
     3961 cagaccctag agaaagcaaa atttctgagg atcatttaat cgaatcgatg aaaaaaacgg
     4021 atgactctta tctgttgttc gatacagaac cgacgccgta tcaagaaaaa agaaatggtg
     4081 agccgtctgt acgaacattc aatcgaacaa aaacgttaga ggaaacaaag cagtcagaag
     4141 aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc attacaagga ttgatcgatg
     4201 atcaaggaaa ctctttggga gaaaatagct attttaaata agtgatatac aaatttatat
     4261 tcggctacag ttggaaagga aacgctgcgg cgtttcctta ttttatactc tatccgaaag
     4321 tttatatcgt atcctcaaat ttttttgagt ttaagcgaat tttttctccc aataagtaag
     4381 aaaataatgg caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa aaatagttta
     4441 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
     4501 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
     4561 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
     4621 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
     4681 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
     4741 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
     4801 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
     4861 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
     4921 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
     4981 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
     5041 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
     5101 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
     5161 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
     5221 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
     5281 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
     5341 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
     5401 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     5461 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     5521 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     5581 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     5641 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     5701 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     5761 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     5821 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     5881 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     5941 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     6001 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     6061 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     6121 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     6181 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     6241 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     6301 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     6361 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     6421 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     6481 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     6541 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     6601 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     6661 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     6721 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     6781 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     6841 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     6901 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     6961 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     7021 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     7081 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     7141 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     7201 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     7261 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     7321 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     7381 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     7441 aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc
     7501 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     7561 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     7621 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     7681 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     7741 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     7801 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     7861 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     7921 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     7981 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     8041 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     8101 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     8161 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     8221 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     8281 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     8341 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     8401 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     8461 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     8521 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     8581 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     8641 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     8701 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     8761 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     8821 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     8881 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     8941 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     9001 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     9061 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     9121 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     9181 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     9241 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     9301 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     9361 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     9421 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     9481 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     9541 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     9601 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     9661 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     9721 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     9781 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     9841 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     9901 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     9961 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
    10021 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
    10081 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
    10141 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
    10201 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
    10261 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
    10321 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
    10381 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
    10441 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
    10501 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
    10561 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
    10621 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
    10681 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
    10741 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
    10801 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
    10861 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
    10921 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
    10981 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
    11041 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
    11101 cacaatttgg tgtaaattat caagaagatg tcacttatgc tcacgcagat ggacagaata
    11161 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
    11221 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
    11281 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
    11341 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
    11401 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
    11461 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
    11521 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
    11581 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
    11641 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
    11701 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
    11761 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
    11821 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
    11881 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
    11941 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
    12001 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
    12061 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
    12121 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
    12181 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
    12241 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
    12301 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
    12361 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
    12421 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
    12481 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
    12541 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
    12601 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
    12661 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
    12721 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
    12781 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
    12841 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
    12901 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
    12961 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
    13021 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
    13081 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
    13141 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
    13201 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
    13261 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
    13321 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
    13381 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
    13441 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
    13501 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
    13561 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
    13621 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
    13681 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
    13741 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
    13801 a
//
LOCUS       DAZUKD010000070        13733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000070 DAZUKD010000000
VERSION     DAZUKD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.071"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..1268)
                     /gene="mobT"
                     /locus_tag="ACG8N9_001809"
     CDS             complement(63..1268)
                     /gene="mobT"
                     /locus_tag="ACG8N9_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4897382.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="ACG8N9_001810"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="ACG8N9_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="ACG8N9_001811"
     CDS             complement(1447..2832)
                     /locus_tag="ACG8N9_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4897383.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="ACG8N9_001812"
     CDS             complement(2861..3247)
                     /locus_tag="ACG8N9_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4897384.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="ACG8N9_001813"
     CDS             complement(3263..3577)
                     /locus_tag="ACG8N9_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4897385.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="ACG8N9_001814"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="ACG8N9_001814"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4897386.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="ACG8N9_001815"
     CDS             4837..6126
                     /locus_tag="ACG8N9_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4897387.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /gene="ytpR"
                     /locus_tag="ACG8N9_001816"
     CDS             complement(6165..6770)
                     /gene="ytpR"
                     /locus_tag="ACG8N9_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4897388.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="ACG8N9_001817"
     CDS             complement(6966..7430)
                     /locus_tag="ACG8N9_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4897389.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="ACG8N9_001818"
     CDS             complement(7452..7772)
                     /locus_tag="ACG8N9_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4897390.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="ACG8N9_001819"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="ACG8N9_001819"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4897391.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="ACG8N9_001820"
     CDS             9194..9544
                     /locus_tag="ACG8N9_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4897392.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="ACG8N9_001821"
     CDS             9729..10682
                     /locus_tag="ACG8N9_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4897393.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="ACG8N9_001822"
     CDS             10695..12482
                     /locus_tag="ACG8N9_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFS4897394.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="ACG8N9_001823"
     CDS             12469..13671
                     /locus_tag="ACG8N9_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897395.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2520 g         4586 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gag
//
LOCUS       DAZUKD010000071        13703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000071 DAZUKD010000000
VERSION     DAZUKD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.072"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..274
                     /locus_tag="ACG8N9_001824"
     CDS             <1..274
                     /locus_tag="ACG8N9_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897396.1"
                     /translation="TLNTTELTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVR
                     KNQKTVVYGISLLILSIIPFFLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(770..842)
                     /locus_tag="ACG8N9_001825"
     tRNA            complement(770..842)
                     /locus_tag="ACG8N9_001825"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(807..809),aa:Lys,seq:ctt)
     gene            1223..1792
                     /locus_tag="ACG8N9_001826"
     CDS             1223..1792
                     /locus_tag="ACG8N9_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4897397.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1779..2639
                     /locus_tag="ACG8N9_001827"
     CDS             1779..2639
                     /locus_tag="ACG8N9_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4897398.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2829..3533
                     /gene="yycF"
                     /locus_tag="ACG8N9_001828"
     CDS             2829..3533
                     /gene="yycF"
                     /locus_tag="ACG8N9_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4897399.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3538..5373
                     /gene="walK"
                     /locus_tag="ACG8N9_001829"
     CDS             3538..5373
                     /gene="walK"
                     /locus_tag="ACG8N9_001829"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4897400.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5370..6686
                     /gene="yycH"
                     /locus_tag="ACG8N9_001830"
     CDS             5370..6686
                     /gene="yycH"
                     /locus_tag="ACG8N9_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4897401.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6687..7556
                     /locus_tag="ACG8N9_001831"
     CDS             6687..7556
                     /locus_tag="ACG8N9_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4897402.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7611..8420
                     /locus_tag="ACG8N9_001832"
     CDS             7611..8420
                     /locus_tag="ACG8N9_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4897403.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8523..9152)
                     /locus_tag="ACG8N9_001833"
     CDS             complement(8523..9152)
                     /locus_tag="ACG8N9_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4897404.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9322..10614
                     /locus_tag="ACG8N9_001834"
     CDS             9322..10614
                     /locus_tag="ACG8N9_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4897405.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10690..11352)
                     /locus_tag="ACG8N9_001835"
     CDS             complement(10690..11352)
                     /locus_tag="ACG8N9_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4897406.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11577..11861
                     /gene="groES"
                     /locus_tag="ACG8N9_001836"
     CDS             11577..11861
                     /gene="groES"
                     /locus_tag="ACG8N9_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4897407.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11912..13537
                     /gene="groL"
                     /locus_tag="ACG8N9_001837"
     CDS             11912..13537
                     /gene="groL"
                     /locus_tag="ACG8N9_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4897408.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4564 a         2335 c         2923 g         3881 t
ORIGIN      
        1 tactttaaat actacagaac taaccagtaa ggatatagat atgttagtca atctaagcgg
       61 gaatgttgtt ttatttggag gaattaccct agttttttcg ataatgattt tcataatagg
      121 ttttttagta agaaaaaatc aaaaaacagt ggtctatggt attagtttac tcattttatc
      181 tattattcca ttttttcttt taacattatt gtgggtaatt cctggagttt taggtattat
      241 tgcgggattg atgcttctaa taagaaaatc ttaattaggc aatgtgtaga caacgtgaaa
      301 cgattaatca attaaaagct aaatacgttt tgagaaatga taacttgtag tcgatggaag
      361 acatgtagat aaaaaaacta cgtgtctttt atttattcta attttttcga aaaaaagaca
      421 ctctctgatt gttgtattaa tggaaaaggg gaaagcagca agtaaattta gtttttctat
      481 taaatattct tcacttttta accttttata gtacttagat ttaatcctat atgtttagag
      541 agttcattgg cagcatgatt ttgcaaagct tctcgaatta ttgttatatt agaaacttta
      601 ctgttcataa ttatgcttct tttttaactc aaagtccaaa gggctacagt ggactttgag
      661 ttaaaaataa aaagccctag ttattttgaa aaataaaaaa agaacagcgt aaatgttttc
      721 gaaatgcttt cgcaccaaca tttacctgtt ccgtgggttt ggcacccaat gggccgtgag
      781 gggctcgaac ccgcgacccg ctgattaaga gtcagctgct ctaccaactg agctaacggc
      841 ccgtacaaaa agtacttaat aatagtagca ctaagaattt taaaatgcaa gactacattt
      901 aaagaaaatc agaaaaatct ttacttcctt ataggaaatc attcatagat tattcacatt
      961 tgctcgctat tatataagca taccaacaaa caaagttctt gcaagagaac aaaccctaac
     1021 acttttttca ttttttactc ctttcccgcc gtctccccgg cgggtctcct gttcttaacg
     1081 ataaaagaca agaaggctta gccttcttgt tttttgtttt catagaatcc cgctaatcaa
     1141 atcacctagt taaagcaact agattgaatg tttatcataa gtctgatatc atgtttatga
     1201 aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag caattaatgg agtgggggaa
     1261 ggaactagaa acattccgtc tgcctcactg ggaagaactc cctgacttag aactttatat
     1321 ggaccaagtc agaacgttag ttgatcgtta tctttcacct gtgatacaag gagaaaagca
     1381 tccattgctt acctcttcta tggtaaacaa ttacgttaaa ctaggtttga taccagcacc
     1441 agttaaaaaa agatataaca aagaacacgt agcattttta cttgcaatca ctacgttgaa
     1501 acaagtattg actattcctg aaatcaaaga agggatattg ttccaaggaa aaacagtagg
     1561 aattcgtgaa gcctataatc tattttgcga tgaacaagaa gcagctgtgt ggatggtcag
     1621 ccaattagca caaggtaaat ctcatccgca aaaattcgac caggcaaccc ctgtagaata
     1681 tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa cttcttgcgg aaaaaacgat
     1741 tgttctagaa accgaatatt taaaagagga gaaaacaaat gaaaaacaat aaaatcgcaa
     1801 ttttagtaga ttcaggtacg gatgttccag aagaattgat aaaaaaatac gagatgtata
     1861 tgatcccatt gaaaattatt tataaagatc aggtatatac agataaaatt gacattaccc
     1921 ctgaagagat ctatcaacgt ttgccagagg aaatccctgg tacatctttg ccggatggtg
     1981 agtcgatcac agaaatcttt gagcaaatca aaaaagacgg ttatgaaaag gtacttgcgg
     2041 taacaatttc cagtggattg agcgggacat ataatgtggt tcgtttaatt gcacaacagc
     2101 aagaagaatt ggaagcatat gtactagata cgaaaaatat tggtattggt gcaggttttt
     2161 ctgcgatcca agcagcaaaa tggctggaag aaggagtaga atgggatcaa ctgatcagta
     2221 atctgaatga gttagtcgaa agaacaaaag ttttctttaa tgttgcgaca ttggaatatc
     2281 ttcaaaaagg tggacggatc ggtttagttg cttctattgt aggaaccgct ttaaagctta
     2341 acccaatcat ctcatgtaat gaagaaggga tttattacac agtaggaaaa gctaggggac
     2401 gtaaaaaaag tttggataaa acggttgctt acgtcaaaga aaaaatcggt aatgcaaagg
     2461 tcttcaactt agctgttgcc catggggatg cgaaagaaga agcaattgaa atggaagcga
     2521 gacttaaaag agagttccca caagcaaacc aaatctactt tggccagatt tctccggcat
     2581 tagtagtcca cactggtccg ggattattgg gtgtcggtgt acaagtttta gaaggataaa
     2641 tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc ttttttattg aagttagaac
     2701 aaaaaataat aaagtatttt gcactttcat tttaattttc tcttttgttt tataggcaaa
     2761 gatgaaacaa aagcagaaat acggtataat gagaaggtat gacaataata tataagaggt
     2821 ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa gccgatttcg gatatcgtca
     2881 aattcaattt agccaaagaa ggctatgacg tttatactgc atatgatggg gaagaagcct
     2941 tggaaaaagt tgctgaggta gaacctgatt tgattttact tgatttgatg ctacctaaaa
     3001 tggacggctt agaagttgct cgtgaggtac gcaaaactta tgatatgccg atcattatgg
     3061 taacagccaa agattctgaa attgataaag tcctaggctt agagttaggc gccgatgatt
     3121 acgtgaccaa gccattttcc aaccgagaat tagttgcacg tgtcaaagca aatcttcgtc
     3181 gcggtgccac ggctgcgaag gaaccagaag aggcagcacc tgcggaattg acgattggtg
     3241 atttgacgat ccatccagaa gcttacatgg tgacgaaacg aggagaaaca atcgagttaa
     3301 cacatcgtga attcgaacta ctcttttact tggcgaagca ccttggacaa gtgatgacaa
     3361 gagaacattt attacaaacg gtttggggtt atgattattt tggagatgta cgaacagtgg
     3421 atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa tccaagccat ccaaattatt
     3481 tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc agaacaggag taattggatg
     3541 aaaagaaaag tccgcttttt tcgatcagtc aattttaaga tcgctattac ctttattctg
     3601 attttattga tctcgatcga aattattggt gcctacttta tacgtggttt agaacgttcc
     3661 acgatcaata catttattaa agatatgaat caaacggtgg aatcattagc aacaacaatc
     3721 agcccggaat tgaatcgaaa agataatgca gatgatgaag aggtaaatgc gaatatcaaa
     3781 agatttattg aaaacagtgc gactagcgat atcatcgaaa tacgggtcgt tgacgaaaaa
     3841 gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg ctgtgggaaa gaaaaatgat
     3901 tatgttgata tcaatgattt cacgactaaa cgttatgttg ctttagataa cgacgataaa
     3961 cgggtgaata tcaatgttca accgatcctg tccccgaccg gcgatgccgt tatcggggct
     4021 ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa tcaataacac agcagtcatt
     4081 ttctttactg cctcactaat tgcggcattt atttcaatga tcgtatctgt tttagttgct
     4141 cgttcgatta cacagccgat cggtgaaatg agagaacaag ccattcgaat cgctagagga
     4201 gactacagtc ggaaagtcaa agtgcatgga caagatgaac ttggacagct ggctgatacc
     4261 ttcaatcagt tagctgaacg tattgaagaa gctcaagata caatggaagc tgaacgcaat
     4321 cgtttggata gcgtgctatc ccatatgaca gacggcgtca ttgcgacaga tcgcaggggt
     4381 aaagtcatta cgatcaatga tatggctgtt tccttgctag atatcaaaaa cgaggaagca
     4441 atcggacaat cgattttgac acttttagat atcgaagaag aatatacgtt gcggaaatta
     4501 ttagaagatc caaacgaaat ggtacttgac cgttcaacca gtacattgga aagtgatcag
     4561 atgatcttgc gagtagactt ttctatgatc cgtcgcgaat caggttttat cagcggatta
     4621 gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag aacgggaacg tcgtgaattc
     4681 gtctctaatg tttcccatga acttcgtaca ccgttgacaa gtatgcgtag ttatatcgaa
     4741 gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac caaatttttt gaaggtaacg
     4801 ctagatgaaa cagaccgaat gattcgaatg atcaatgacc tcttaaatct atctcgcatg
     4861 gatacaggta atacccagct tcaattagaa tatgtcaatt tcaacgagtt agtgaatttt
     4921 gttcttgacc ggtttgatat gatggtggga aatcaggaaa aaaattataa gatccgtcgt
     4981 gaatttacgc aaagagatct ttgggtcgaa gtagatacag ataaaatcat ccaagtagtc
     5041 gataatatca tgaataacgc gatcaagtat tcaccagatg ggggaacgat tacttgtcgc
     5101 ttattagaaa cgcataataa cgtgatttta agtatcaccg atcaaggatt ggggattcct
     5161 aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag tagataaagc acgtgcaaga
     5221 gcacaaggtg ggacaggact tggattagct atttctagag aagtgattaa ggcgcatcga
     5281 ggcgctattt gggcagagag caaggaagga aaagggtcga ctttttatat ctcgctgcca
     5341 tatgaacctt atgaggagga atggtgggaa tgagaatctc tgaaaaagtc gttcgtatag
     5401 gactcatttt gatggttgcg ttaagcatat acttctctta tgcaatctgg ctgagcccag
     5461 caggaaaaag ttcaatcaac gtagatgaga caaacactca aatgatcgag tctcaaagca
     5521 atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac atggcaccat tcgggaatga
     5581 tccaagaaac caacagtgaa aatctcgtgt accgattgca attgatcatc gataaagcaa
     5641 gatttggaaa gctttctgaa gtagtttcag gagacaaatc acagtttgaa caggtgaaaa
     5701 atctgaactc tggttttgag ttggcctata atgcgccttt tttgctaaga gaatacaagg
     5761 aagcatttga tttagattta gatgtgtcga acttggcaaa agaaagcgaa aatatgtatt
     5821 ttactagaat acaattcgat caaggaaata aaaaaatccg atttttgaat tattcgaacc
     5881 atctcgtata tgaagcaaat atttcgttga attggacaga tatcaataaa gaattaacag
     5941 caaaagacac aaaatggaca gaaatgacaa cagaaccatc gacgatcgac acgcaatatg
     6001 ctacgaaaca ttcgatcaaa ctgaagaagt atagttatat cctgtcacag caaccctata
     6061 cactttttag aaatgctttt ttccagaaac cagatagcgt gaaaaataat gatgaaagca
     6121 atgaattgat tttttacggt gggaatgaaa caatgtctat ccattcggaa acgcaagtag
     6181 ttgattttcg tggagagctg ccagagaaaa aagaaaatgc tagtgtattt tctgaaagtt
     6241 tcccctatat tagcaaatta ggcagttctt tagggaacct cagatatttt gaccgcagtg
     6301 acaatacgat cgattatcgg atattcgtgg aaggcttccc ggtttttggg acagacagca
     6361 gaggacagat tggtgtggaa gttggcggtg aaacgaatgg ttctgtgcaa gtgaatatcc
     6421 aaaccagttt gaatacgatt caagtgccga taccttcaga cgaagaagta gaacttgcaa
     6481 gtacgaatga aatcatccag cagttaaaag ctaagggagc agatagcaac aagattggtt
     6541 cagtgatcgt tggctataca tggcaaaatg tcaaagaaac gaatcgtgtc gttgatctct
     6601 tacctgaatg gtatgtgaaa tataaagata actggtattc agctaacgac cttctggaaa
     6661 aattacctgg attggaggcg aattaattgg atttcaaaaa aatcgaatgg atatttttcg
     6721 tcgctttttt agggttgaat gtgtttttat tcagcagtta tcatgacgcg gtataccaag
     6781 aaaacaatgt cattcgctct aatcagactg aatcaatcca acaacgtctg gctagcgacg
     6841 atatcactta cacggggaag atttcgtctg agaacaagca aggctattat cttagcgcgg
     6901 agcagaccaa tcttagcact tcattagcga attatcgcaa ggctggtaaa agcggcttgc
     6961 tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa atctttatcc agttcagatg
     7021 ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc cttggaagat tttttatcta
     7081 acaaagacaa tattttattc ggtaatgatt atgtgtacac gaaaaacttt tctcatttag
     7141 aagaagaacc actagaaatc caagcaagcc aagagtatga aggcatccca ttcaatgatg
     7201 atacagcaaa aatcgttctt tctttagaaa aaaacgataa tgattatagc atcacgaaat
     7261 acatgcagac gcatttatct gatatcgagc agctgcgcga aaagacggaa ctgtatacgg
     7321 aagaggaagc aatcaataca ttgtacatca ataacaaaat accaagagga tcaaaaattt
     7381 tatggcgtca gctagcttat tcctgtattc tgaaagtccg ggaaaaaaat gtgtatgttc
     7441 ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt ccaaattgag agcgtcaacg
     7501 ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa agtggaagat caataaaaaa
     7561 gcgagtataa tgaaaaaagt ttacaaagta agaagggaag taggagtcaa atggctgaag
     7621 aaaaaccagc ttttaaaatc agtatcctag caagtggaag ttcgggtaat tcactttata
     7681 tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact tagcggtaaa aaaatcactt
     7741 ccttgatggc tcaaattgat cggacaccgg accaattaga tggaatactt gtgacgcatg
     7801 aacatcgaga tcatatacat ggcgtgggtg tcttggctcg taagtatcat ttggatgttt
     7861 ttgccaatca gcagacttgg gaagcgatgg aaccactgat tggaaatgta ccagtggaac
     7921 aaaaacatct atttgagatg gggaaagtca gaacattcgg cgatatcgat attgagagtt
     7981 tcggcgtctc ccatgacgca gcggctccgc aattttatcg tttccataaa gaaaatcggt
     8041 cattcgtcat gttgacggac accggatatt gcagtgatta tatgcgaggt gtcattgaaa
     8101 atgccgatgg ttatttgatg gaaagtaatc atgatcttga gatgttacgc atgggacctt
     8161 atccgtggaa tttaaaacag cgtattttag gagatagagg acatctttcc aacgaagacg
     8221 gtgcacttgt catgactgat gtgatcggta atcggaccaa acgaatctat cttggccatc
     8281 ttagcaagga aaacaatatg aaagaacttg ctcatttaac aatggaaaat gttttgaaag
     8341 aaaaagattt tggcgttgga catgattttg aaatctatga tacagatcca gatgaagcaa
     8401 ctgagctatt ttcgatttaa acagttcaat caataaaaat ataaaaagca gaagataaaa
     8461 tcaatcaggg ttggttttat cttctatttt tattttttta gcatattaaa aaaataaccg
     8521 tattatcgac tgactacact gatgtgctgc ttttcatgtt tctcatccag tatctcatct
     8581 actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt cgctagcagc gtttctttgt
     8641 aaataatcat cacttaacaa ataagtacct gtacgtttgc catgtggatc aaataaagaa
     8701 gctggactga tataagtcca atgtagattt ttagattgtt tcaattttaa caatccttcg
     8761 tacatattga aagctgttgg atagaagggt gcatttttag gtacttggtc aatcatacga
     8821 gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc caacgacaat taagcgtgta
     8881 tcggttcctt caagcatctc aatcaaatgg gacaaagaag tttgatgcat ttcttctttt
     8941 cctttgggag cgttgaaagc atcaacgaca acatcaaaag atgcaagatc agtagttgtc
     9001 aactcaaata aatccttttg tataaacgga acatctgatg gcaatttttc tggatgacgc
     9061 acgattgccg tcacgtctaa atctctggac aatgcttcgt ctaaaataaa tgaacctgca
     9121 tggccagtag ctccaatgag tgcaattttc ataaaaaacc tcctttttta ttcctgtcca
     9181 ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt ttgaaaagct cagtgcgcat
     9241 tcctttcatg gttcgtgatc attgggtata gttaaattaa gaaaactgat tgataaaaat
     9301 agaagttgaa ggagataaga aatgaaagag tggttagatc gtgccttttt accaatgata
     9361 acatctgttg tgaatcttag acccttaact gctttgcgta atggaatgac ttacacgatc
     9421 ccaataatta ttgttggctc gctgtttttc ttatttgctc attttcctgt ggattacatc
     9481 gctcagtgga tagacaaaac aggattttct atgtatttta cacaagtata ccaggctaca
     9541 tttgatgtga tagccttttg gatcgttttt gccattgcat acgcttatgc aaaagaagaa
     9601 gacatcgatc gcttaccagt cggcataaca gccttgtctg cttatctact gcttattcag
     9661 ccactaaata ctcagataga tactgtctgg acaggtgctg aagggatact aggagcagtt
     9721 acaactgggc tcatcatcgg atggggctat gcacgtttag taagacttgc gaaagggtgg
     9781 aagcaaagag agaagatacc tgaaaatgtt tggcagtctt ttacttcatt gattccaatc
     9841 attgttatag tcacttttac gcttatcggt tcagcggttt ttcaacattt gttcagaatg
     9901 actgttgttc aaggagtatg gacatatgtc cagcttccca ttcaatcgtt catagacacg
     9961 ttgtttggtg tgcttttatt aggttttttt gttccgtttt tatggttatt tggtcttcat
    10021 ggatctgcga tggtcaatgg gttggttagt cccgtcttgc aagcgaattc attagcaaat
    10081 gcagaaatct tagcctcagg acaagagttg acggtggcaa atggcggaca tatcgttact
    10141 cagcaatttc tcgatcagtt catgacggtt acgggtgctg gattgacgtt aggggctgta
    10201 ttttttatga cggtttttgc gaaatcgaag aaatacagag aattaggaaa tcttgctctt
    10261 ttaccagcct ttttcaacat caatgaatca attatttttt ccacaccgat cgtgatgaac
    10321 ccgatgatgg ctgttccttt tattttcgca ccggtcgttt caggattgat tacctattcc
    10381 gctttatatt ttggattcgt tcctttattt acagcagtcc aagtcccttg gacaacaccg
    10441 ccgatcctct cgggtttctt agtaggcgga gtgcctgcag cgattttaca aggtatcgtg
    10501 ttgtcaatta gttttttcat ttatttccca tttttgaaaa aaatagattc tgcaaactgc
    10561 aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa agatgaacga ctaaaaaaca
    10621 aaaatactcc tgtcgcttta gattgggaaa atcaccaatc taggatgaca ggagtattta
    10681 tagtcatgtt tatactagcc ctttttggac agctaattga acaagaacaa ctaatgtgtt
    10741 cattcctacg tgtgtgatca tcgatgtcca gatccgacca gttgctttgt actctaggct
    10801 gaagaagaaa cctaggaaaa agtagattag cagatgacca tcattatgag caaacgcaaa
    10861 cagcaaagag ctgatcactg ctccgaccca aaaatttgaa tatcgactga tgatccccag
    10921 tatactgcgg cggaatacga actcttccat gatgggtcca gcaacagttg tcgccaaaat
    10981 aaaagcgggt gcttccatga tcatctggat gatattttgt gtattctcgg aaggggttgc
    11041 tttgccaaac aatacagatt ctaacatgac agcgatgctt tgcagaatca atgcgacaaa
    11101 gattccaacc aatccataaa taacaatctt tggagcagaa gcttttttta ccgctgattc
    11161 gatcgctaag ggttcacgtt gcttaaaata aaaaatagct aggacaacgg cgcctagtat
    11221 atacatgact gcagttgctg tgatcaattc agacgtagat tttacaagtc ctatcgaagc
    11281 taggatcaag ggtgagataa aaattagacc atagcaaaaa atgctgagga agctatattt
    11341 ttttaaagac atgtaattcc tccttttaag ggataacaag catgcaaggc aagcttatat
    11401 cgattgattt caattatatc ataatttcaa taagcagctg tcagaataaa atttcttttc
    11461 tttgatttaa aagcttgcaa aaaagaggga gaatgagtat tattaaaaat gtaggttagc
    11521 actcgaaggt taagagtgct aacagattaa aaaaagagtt atcggaggga tttatcgtgt
    11581 taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa aaaactgtcg
    11641 gaggaatcgt tttagcttct gctgctaaag agaaacccca aactgggaca gttgttgcag
    11701 tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta aaagcaggcg
    11761 accaagttat gttcgaaaaa tacgccggta cagaagtaaa atacgaagga aaagaatatc
    11821 tgatcgtcgc aggaaaagat atcatggcga ttgttgagta aatttggaag ataaaaaaat
    11881 aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa gaattgaaat ttgcagaaga
    11941 cgcacgcgca gctatgttac gcggggtaga taaattagca gatacagtga aagtaacatt
    12001 aggacctaaa ggccgaaatg tcgtactaga aaaatcttac ggctctccat tgatcacaaa
    12061 tgatggtgtg acgatcgcaa aagaaatcga attagaagat cattttgaaa acatgggtgc
    12121 aaaacttgta tcagaagtag catcaaaaac aaatgatatt gcaggagacg gtacaacgac
    12181 agcaactgtt ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa ctgcaggtgc
    12241 taatcctcta ggcattcgtc gcgggatcga attggcaaca aaagcagcag tcgaagaatt
    12301 gcataatatc tcaactgttg ttgattcaaa agaagccatt gcacaagtag cagctgtttc
    12361 ttcgggttct gataaagttg gacatttgat tgctgatgca atggaaaaag ttggaaacga
    12421 cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca gaactagatg ttgtagaagg
    12481 tatgcagttt gatcgtggtt acttatcaca atatatggta acagataacg acaaaatgga
    12541 agctgttcta gaaaacccat atatcttgat cacagacaaa aaaatctcta acatccaaga
    12601 tattctgcca ttgttagaac aaatcttgca acaatcacgt ccattgttga ttattgctga
    12661 cgatgtagat ggtgaagcat tgccaactct tgtattgaac aaaatccgtg gaacattcaa
    12721 cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc ttgaagatat
    12781 cgctattttg acaggtggta cagtaattac tgacgatcta ggtttagaat tgaaagatgc
    12841 aacaatcgaa aacttaggaa atgcttctaa agttgttgta gataaagaca atacaacgat
    12901 cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt gtgcaattga tcaaaaacca
    12961 aattgctgaa acaacttctg attttgaccg cgaaaaatta caagaacgct tagctaaatt
    13021 agcaggtggt gtagctgttg ttaaagttgg tgcagctact gaaacagaat tgaaagaatt
    13081 aaaattacgc attgaagatg cattgaatgc tacacgtgcc gcagtagaag aaggaatggt
    13141 ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg tagaagcaga
    13201 aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac caattcgtca
    13261 aatcgctgaa aacgctggtt atgaaggatc agtgattgtt gataaattga agaacgtgga
    13321 actaggcact ggtttcaatg ctgctactgg agaatgggta aacatggttg aagcaggtat
    13381 cgtagaccca actaaagtaa cacgttctgc cttgcaaaat gccgcttccg tttctgcctt
    13441 gttattaacg acagaagcag tcgttgcaga taaaccagaa ccagcagcgc cagcagctcc
    13501 tgcaatggat ccatcaatga tgggcggtat gatgtaagaa aaagcaactt gcttatctac
    13561 aactgatcat tagtgaaaga cagataaaaa gaggctggaa cagaagcgtt taactccaag
    13621 aaataagaag aaattcacgg aaattgcttt tcaaattttt gtgaatttca gcttatttcc
    13681 gaaggagttg cttctgcttc cgc
//
LOCUS       DAZUKD010000072        13297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000072 DAZUKD010000000
VERSION     DAZUKD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.073"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8N9_001838"
     CDS             136..309
                     /locus_tag="ACG8N9_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897409.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG8N9_001839"
     CDS             949..1422
                     /locus_tag="ACG8N9_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4897410.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACG8N9_001840"
     CDS             1431..1658
                     /locus_tag="ACG8N9_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897411.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACG8N9_001841"
     CDS             2294..2665
                     /locus_tag="ACG8N9_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897412.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACG8N9_001842"
     CDS             complement(2921..3163)
                     /locus_tag="ACG8N9_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4897413.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACG8N9_001843"
     CDS             complement(3195..4097)
                     /locus_tag="ACG8N9_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4897414.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACG8N9_001844"
     CDS             complement(4110..4298)
                     /locus_tag="ACG8N9_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4897415.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8N9_001845"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8N9_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4897416.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACG8N9_001846"
     CDS             complement(4913..5806)
                     /locus_tag="ACG8N9_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4897417.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACG8N9_001847"
     CDS             complement(5884..6807)
                     /locus_tag="ACG8N9_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4897418.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACG8N9_001848"
     CDS             complement(6856..7206)
                     /locus_tag="ACG8N9_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4897419.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACG8N9_001849"
     CDS             complement(7239..8141)
                     /locus_tag="ACG8N9_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4897420.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACG8N9_001850"
     CDS             complement(8134..8991)
                     /locus_tag="ACG8N9_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4897421.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACG8N9_001851"
     CDS             9325..10134
                     /locus_tag="ACG8N9_001851"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4897422.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACG8N9_001852"
     CDS             10174..10671
                     /locus_tag="ACG8N9_001852"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4897423.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ACG8N9_001853"
     tRNA            complement(11056..11129)
                     /locus_tag="ACG8N9_001853"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="ACG8N9_001854"
     CDS             11316..11639
                     /locus_tag="ACG8N9_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897424.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="ACG8N9_001855"
     CDS             11803..12057
                     /locus_tag="ACG8N9_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897425.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="ACG8N9_001856"
     CDS             12127..12372
                     /locus_tag="ACG8N9_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4897426.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="ACG8N9_001857"
     CDS             12448..12813
                     /locus_tag="ACG8N9_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4897427.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..>13297)
                     /locus_tag="ACG8N9_001858"
     CDS             complement(12874..>13297)
                     /locus_tag="ACG8N9_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897428.1"
                     /translation="LFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDY
                     RGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNH
                     VRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3868 a         2459 c         2235 g         4735 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagt
//
LOCUS       DAZUKD010000073        13212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000073 DAZUKD010000000
VERSION     DAZUKD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.074"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8N9_001859"
     CDS             147..1016
                     /locus_tag="ACG8N9_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4897429.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8N9_001860"
     CDS             1225..2250
                     /locus_tag="ACG8N9_001860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4897430.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8N9_001861"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8N9_001861"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4897431.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG8N9_001862"
     CDS             3333..5036
                     /locus_tag="ACG8N9_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4897432.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8N9_001863"
     CDS             5038..7071
                     /locus_tag="ACG8N9_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4897433.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8N9_001864"
     CDS             7082..7522
                     /locus_tag="ACG8N9_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897434.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8N9_001865"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8N9_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897435.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8N9_001866"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8N9_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4897436.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG8N9_001867"
     CDS             9793..11559
                     /locus_tag="ACG8N9_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4897437.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG8N9_001868"
     CDS             11574..12011
                     /locus_tag="ACG8N9_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897438.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG8N9_001869"
     CDS             12024..13175
                     /locus_tag="ACG8N9_001869"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4897439.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZUKD010000074        13097 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000074 DAZUKD010000000
VERSION     DAZUKD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.075"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1800
                     /gene="yfcC"
                     /locus_tag="ACG8N9_001870"
     CDS             61..1800
                     /gene="yfcC"
                     /locus_tag="ACG8N9_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4897440.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1957..3141
                     /gene="iadA"
                     /locus_tag="ACG8N9_001871"
     CDS             1957..3141
                     /gene="iadA"
                     /locus_tag="ACG8N9_001871"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4897441.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3252..3455
                     /locus_tag="ACG8N9_001872"
     CDS             3252..3455
                     /locus_tag="ACG8N9_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4897442.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3556..4218)
                     /locus_tag="ACG8N9_001873"
     CDS             complement(3556..4218)
                     /locus_tag="ACG8N9_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897443.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4231..5733)
                     /locus_tag="ACG8N9_001874"
     CDS             complement(4231..5733)
                     /locus_tag="ACG8N9_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897444.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6281..7960
                     /locus_tag="ACG8N9_001875"
     CDS             6281..7960
                     /locus_tag="ACG8N9_001875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4897445.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8087..8566
                     /locus_tag="ACG8N9_001876"
     CDS             8087..8566
                     /locus_tag="ACG8N9_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4897446.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8785..9465
                     /locus_tag="ACG8N9_001877"
     CDS             8785..9465
                     /locus_tag="ACG8N9_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4897447.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9473..10804
                     /locus_tag="ACG8N9_001878"
     CDS             9473..10804
                     /locus_tag="ACG8N9_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4897448.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10819..11781
                     /locus_tag="ACG8N9_001879"
     CDS             10819..11781
                     /locus_tag="ACG8N9_001879"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4897449.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11892..12230
                     /locus_tag="ACG8N9_001880"
     CDS             11892..12230
                     /locus_tag="ACG8N9_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4897450.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12309..13016
                     /locus_tag="ACG8N9_001881"
     CDS             12309..13016
                     /locus_tag="ACG8N9_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4897451.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4160 a         2316 c         2877 g         3744 t
ORIGIN      
        1 catttgatta tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat
       61 ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc
      121 catacgtatg tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt
      181 ccagcaggaa agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt
      241 actcgtactg ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat
      301 gtgtttgatc aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt
      361 ccggaaaaag gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa
      421 ctagatgaaa tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat
      481 gatacttcta aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc
      541 tttggtttct taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct
      601 gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc
      661 ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta
      721 gctttaccgc tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa
      781 gaggtaattc cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg
      841 atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg
      901 agcccgttta gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg
      961 acattccgat tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata
     1021 tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat
     1081 ttccgttctc atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa
     1141 ttgatcttat tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa
     1201 ggctattaca ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc
     1261 attgctgttc ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa
     1321 tctggtgcag ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt
     1381 cttgtcttgg gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg
     1441 atcgggacgg cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc
     1501 caaagtttgt tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca
     1561 atcatggctc cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat
     1621 cagcttggtt caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg
     1681 ctaggtgttg cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga
     1741 ttcctgtttg ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag
     1801 tactggagac ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt
     1861 cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc
     1921 tatcatcagt tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa
     1981 gtgtttgcac cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg
     2041 gctgtagaag aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt
     2101 gagggaaaag tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga
     2161 ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct
     2221 ggtgtaacga cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg
     2281 ttgatcagca aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt
     2341 tcgtaccgat tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt
     2401 gataaaatta tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt
     2461 tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa
     2521 gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga
     2581 atcgtagaag aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca
     2641 ccagaattat ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact
     2701 gctagcgaag atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca
     2761 ctgaaacgat tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga
     2821 caaggaagct tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt
     2881 gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa
     2941 atcgccttac gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga
     3001 aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt
     3061 gatacagtta tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg
     3121 actttcgaaa aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc
     3181 tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag
     3241 gaggaagcac aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg
     3301 gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga
     3361 aagcacaatc actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca
     3421 aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca
     3481 agcaaataaa aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga
     3541 tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac
     3601 ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg
     3661 ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt
     3721 tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc
     3781 gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc
     3841 aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt
     3901 agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta
     3961 agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag
     4021 tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga
     4081 aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc
     4141 gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa
     4201 ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa
     4261 tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc
     4321 ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga
     4381 aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg
     4441 tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt
     4501 ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc
     4561 aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt
     4621 acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt
     4681 aatcataatg atcagagtaa gtatgataat accggctaca gtcactagta aaacaacatt
     4741 tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt
     4801 tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc
     4861 cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt
     4921 cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa
     4981 gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt
     5041 catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc
     5101 caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct
     5161 gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg
     5221 tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc
     5281 cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc
     5341 agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga
     5401 ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt
     5461 aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg
     5521 ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc
     5581 tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac
     5641 aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt
     5701 tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat
     5761 ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca
     5821 acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg
     5881 aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct
     5941 agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat
     6001 ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag tattctggct
     6061 atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc
     6121 caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa
     6181 tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg
     6241 acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat
     6301 cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga
     6361 tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt
     6421 cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt
     6481 aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt
     6541 accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga
     6601 agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg
     6661 tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa
     6721 tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc
     6781 tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt
     6841 agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca
     6901 aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg
     6961 tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg
     7021 aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga
     7081 aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga
     7141 tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca
     7201 aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat
     7261 cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta
     7321 tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa
     7381 tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca
     7441 aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata
     7501 caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag
     7561 tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat
     7621 cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt
     7681 tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg
     7741 attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac
     7801 aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat
     7861 ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc
     7921 agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac
     7981 ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca
     8041 gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac
     8101 aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca
     8161 tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat
     8221 atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt
     8281 gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt
     8341 tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat
     8401 tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa
     8461 agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga
     8521 tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga
     8581 agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc
     8641 tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt
     8701 tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata
     8761 cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca
     8821 attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg
     8881 acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt
     8941 gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt
     9001 gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt
     9061 ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc
     9121 tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca
     9181 gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt
     9241 gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg
     9301 ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat
     9361 tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat
     9421 agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac
     9481 aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt
     9541 aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga
     9601 tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg
     9661 caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg
     9721 ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc
     9781 gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa
     9841 ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg
     9901 attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg
     9961 cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt
    10021 gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta
    10081 tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc
    10141 gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt
    10201 tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt
    10261 aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt
    10321 catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat
    10381 gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat
    10441 atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa
    10501 gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt
    10561 acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa
    10621 tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt
    10681 agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt
    10741 gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc
    10801 ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac
    10861 gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta
    10921 ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa
    10981 acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa
    11041 ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat
    11101 cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat
    11161 cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg
    11221 atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc
    11281 gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct
    11341 tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg
    11401 ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat
    11461 ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca
    11521 gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt
    11581 tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc
    11641 aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca
    11701 aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat
    11761 aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac
    11821 aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa
    11881 ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag
    11941 atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa
    12001 ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg
    12061 atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag
    12121 gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag
    12181 aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca
    12241 aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg
    12301 aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca
    12361 ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat
    12421 acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac
    12481 aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact
    12541 acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg
    12601 ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg
    12661 tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt
    12721 tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga
    12781 ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg
    12841 cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac
    12901 cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga
    12961 cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata
    13021 aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata
    13081 ccatttattt ggaataa
//
LOCUS       DAZUKD010000075        12982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000075 DAZUKD010000000
VERSION     DAZUKD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.076"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      <1..146
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            294..851
                     /locus_tag="ACG8N9_001882"
                     /pseudo
     CDS             294..851
                     /locus_tag="ACG8N9_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            909..2947
                     /locus_tag="ACG8N9_001883"
                     /pseudo
     CDS             909..2947
                     /locus_tag="ACG8N9_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
     gene            complement(3056..4207)
                     /locus_tag="ACG8N9_001884"
     CDS             complement(3056..4207)
                     /locus_tag="ACG8N9_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4897452.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4361..4891)
                     /locus_tag="ACG8N9_001885"
     CDS             complement(4361..4891)
                     /locus_tag="ACG8N9_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4897453.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4990..5340)
                     /locus_tag="ACG8N9_001886"
     CDS             complement(4990..5340)
                     /locus_tag="ACG8N9_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4897454.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5497..7026)
                     /gene="ahpF"
                     /locus_tag="ACG8N9_001887"
     CDS             complement(5497..7026)
                     /gene="ahpF"
                     /locus_tag="ACG8N9_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4897455.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7036..7599)
                     /gene="ahpC"
                     /locus_tag="ACG8N9_001888"
     CDS             complement(7036..7599)
                     /gene="ahpC"
                     /locus_tag="ACG8N9_001888"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4897456.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7743..8405)
                     /locus_tag="ACG8N9_001889"
     CDS             complement(7743..8405)
                     /locus_tag="ACG8N9_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4897457.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8589..10235
                     /locus_tag="ACG8N9_001890"
     CDS             8589..10235
                     /locus_tag="ACG8N9_001890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4897458.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10226..11308
                     /gene="uxuA"
                     /locus_tag="ACG8N9_001891"
     CDS             10226..11308
                     /gene="uxuA"
                     /locus_tag="ACG8N9_001891"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4897459.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11416..12969)
                     /locus_tag="ACG8N9_001892"
     CDS             complement(11416..12969)
                     /locus_tag="ACG8N9_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897460.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
BASE COUNT         4337 a         2382 c         2419 g         3844 t
ORIGIN      
        1 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
       61 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
      121 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
      181 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
      241 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
      301 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      361 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      421 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      481 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      541 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      601 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      661 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      721 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      781 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      841 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      901 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      961 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
     1021 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
     1081 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1141 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1201 gacttcagat atcgaatcta aagtagataa aaaagacgat tctacttaca gtttttcata
     1261 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1321 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1381 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1441 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1501 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1561 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1621 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1681 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1741 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1801 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1861 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1921 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaatag cttttgacag
     1981 tctaggagga aaagctgcat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     2041 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     2101 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2161 tgctcctgga tctacgttta aaatgattac agcagcaatc ggtctcgaca acggcactat
     2221 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2281 atcttttaaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2341 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2401 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2461 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2521 atatggacag ggcgaacttc taattactcc tatccagcaa gcagcaatgt attctgtttt
     2581 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2641 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gaaaagttgt
     2701 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2761 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2821 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2881 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2941 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     3001 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     3061 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3121 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3181 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3241 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3301 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3361 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3421 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3481 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3541 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3601 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3661 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3721 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3781 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3841 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3901 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3961 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     4021 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     4081 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4141 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4201 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4261 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4321 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4381 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4441 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4501 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4561 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4621 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4681 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4741 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4801 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4861 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4921 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4981 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     5041 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     5101 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5161 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5221 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5281 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5341 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5401 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5461 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5521 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5581 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5641 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5701 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5761 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5821 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5881 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5941 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     6001 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     6061 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6121 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6181 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6241 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6301 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6361 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6421 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6481 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6541 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6601 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6661 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6721 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6781 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6841 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6901 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6961 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     7021 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     7081 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7141 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7201 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7261 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7321 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7381 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7441 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7501 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7561 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7621 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7681 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7741 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7801 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7861 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7921 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7981 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     8041 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     8101 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8161 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8221 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8281 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8341 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8401 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8461 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8521 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8581 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8641 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8701 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8761 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8821 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8881 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8941 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     9001 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     9061 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9121 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9181 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9241 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9301 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9361 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9421 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9481 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9541 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9601 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9661 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9721 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9781 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9841 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9901 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9961 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
    10021 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
    10081 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10141 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10201 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10261 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10321 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10381 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10441 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10501 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10561 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10621 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10681 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10741 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10801 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10861 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10921 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10981 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    11041 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    11101 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11161 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11221 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11281 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11341 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11401 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11461 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11521 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11581 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11641 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11701 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11761 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11821 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11881 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11941 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    12001 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    12061 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12121 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12181 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12241 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12301 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12361 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12421 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12481 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12541 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12601 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12661 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12721 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12781 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12841 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    12901 aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg
    12961 agtttgcatg aaagaggctc ct
//
LOCUS       DAZUKD010000076        12718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000076 DAZUKD010000000
VERSION     DAZUKD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.077"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..105)
                     /locus_tag="ACG8N9_001893"
     CDS             complement(<1..105)
                     /locus_tag="ACG8N9_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase"
                     /protein_id="HFS4897461.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNKTKNIFV"
     gene            104..952
                     /locus_tag="ACG8N9_001894"
     CDS             104..952
                     /locus_tag="ACG8N9_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4897462.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1598..1804
                     /locus_tag="ACG8N9_001895"
     CDS             1598..1804
                     /locus_tag="ACG8N9_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4897463.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(2006..3007)
                     /locus_tag="ACG8N9_001896"
     CDS             complement(2006..3007)
                     /locus_tag="ACG8N9_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4897464.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3012..4925)
                     /locus_tag="ACG8N9_001897"
     CDS             complement(3012..4925)
                     /locus_tag="ACG8N9_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4897465.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5093..5599)
                     /locus_tag="ACG8N9_001898"
     CDS             complement(5093..5599)
                     /locus_tag="ACG8N9_001898"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4897466.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5759..6199
                     /locus_tag="ACG8N9_001899"
     CDS             5759..6199
                     /locus_tag="ACG8N9_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4897467.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6225..7253)
                     /locus_tag="ACG8N9_001900"
     CDS             complement(6225..7253)
                     /locus_tag="ACG8N9_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897468.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(7308..7382)
                     /locus_tag="ACG8N9_001901"
                     /pseudo
     CDS             complement(7308..7382)
                     /locus_tag="ACG8N9_001901"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7385..7741)
                     /locus_tag="ACG8N9_001902"
     CDS             complement(7385..7741)
                     /locus_tag="ACG8N9_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897469.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8039..8932)
                     /gene="sgrA"
                     /locus_tag="ACG8N9_001903"
     CDS             complement(8039..8932)
                     /gene="sgrA"
                     /locus_tag="ACG8N9_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4897470.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            complement(9128..9967)
                     /locus_tag="ACG8N9_001904"
     CDS             complement(9128..9967)
                     /locus_tag="ACG8N9_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4897471.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10152..11043
                     /locus_tag="ACG8N9_001905"
                     /pseudo
     CDS             10152..11043
                     /locus_tag="ACG8N9_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
     gene            complement(11637..12332)
                     /locus_tag="ACG8N9_001906"
     CDS             complement(11637..12332)
                     /locus_tag="ACG8N9_001906"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4897472.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12316..12714)
                     /locus_tag="ACG8N9_001907"
     CDS             complement(12316..12714)
                     /locus_tag="ACG8N9_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4897473.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3855 a         2482 c         1922 g         4459 t
ORIGIN      
        1 tacaaaaata ttttttgttt tattttttcc tactctcctt gtatctttaa cattaatgag
       61 aacggaaaaa atgataatct tattatgaaa atgaggaatt ctcatgagcg tctccataag
      121 actacaaatc aaaacaatgt acaacgactt cagcccaaaa gaacaggcta tcgccgatta
      181 tattttagaa aatccaagca aagtaagtca tagtcctatt agcaatttag caagtgaact
      241 gggcattgct gactctactt tttttcaatt tacaaaaaaa cttggttata atggatttaa
      301 agattttaaa atggccatgt tgatccaaga aaatgatcta tctgctattt ctatccacga
      361 aaatattcag aaaacagata ctgagctcac tatggcgcaa aaggtgttcg agtctagtat
      421 ttcaacttta aatgacacaa aaaagttgtt acaacaaaag gatctaaaaa aagcggctac
      481 tattatcaat gaatctaaac gtgtctattt tttcggtgtt ggcggttcag agattgtagc
      541 aactgacgct tatcataaat ttttgcgttc ccctatcgca acttctcaca gcacggatta
      601 ccatattcaa ttgatggaag cttctttatt gacggaaaaa gactgtgctg ttttgatttc
      661 tcacacaggg caatcaaaag aaacaatcca tattgctgaa accgtaagaa aaactggagc
      721 aaaaacaatc gttattacta gccaagctaa ttcttccctt gctaaacttg gcgatgtcgt
      781 ttttatttct atttctgaag aaacagaatt ccgatctgaa gctttagctt cccgtatttc
      841 tcaactaagc attttggact ctctatatgt gattttgatg ttttacaacg aaaaagaatc
      901 acgtcgttct atcgctaaag ttcgacatac tatttctgaa atcaaagaat agcaccataa
      961 cagaaaaagc gaaaaatcca taaaacaaaa aaagccacta ccaaatattt ggtagtggct
     1021 tttttggttc tgcaagggtg gtacagaaca tcaatagtcg gtgggttaga acgtatgtca
     1081 tcgcatcttt ggagaaggtt gtattggtat tattcgagtt acttgttaga tgcgataaca
     1141 aagttagtat acagtagtaa aaaatttatc ttttcttttt tttattattt ttgtttctta
     1201 ttttctaaaa aattaaaaaa aattttggtg aattaagtat ttccttgata acactggctt
     1261 ttggcctcat tttaggttat catttgtaat tttatttcac agttgatatg attgttatgt
     1321 aaaataaaaa agtttaggat acatcgaatt tttggggaaa ttcttttggg ggtatcctaa
     1381 atcttttcta ttatattccc atattttcta ttattggtct agacgtatga tggtaaactt
     1441 tgtacctatc tgattgctga atcattaatt gcttatcatt agccatcttt gtttcaagtt
     1501 cgctagatga tcattttatt cacatcctaa caaacttgtc acatttatag attcttaatc
     1561 tatttatcat acaattataa aaaaggaggt gctgcttatg aaagctatcc atatttcttt
     1621 gctttttttc gctagtgcat tgattttatt tgctattcat ctttgttccc agccttatat
     1681 caataacaac ggaatattag tggaaccagc attcttttgc ttaccactcg gctttttgtc
     1741 ccttcttgcc tccgcaggct tcggatttgt tgcttttttt aaacgatcaa aacaacaaat
     1801 ctgacttata aaatttacgt aaaagagatc gtgacaataa tcgtgtgata ttcccaaatt
     1861 tcgaataaaa ggtgcccctt gccatctcta actcctcagt cacaagttac aatattcata
     1921 aaccatgaaa agcttgttta ttgaaattgc tccttgtgac ttggagctaa gtgacagatt
     1981 acaacctctt ttgcatcata agactttact ccttgaaaaa cattactaac ttttgatatg
     2041 tttcttgcgc ttgtttattg ctgaaatcaa actgatattc atggttcaaa acagcatttt
     2101 tctccgcata atctactttt gtgactttca cccccgcttt ctcaaataca gaagttactt
     2161 cttccatttg tttcgtaaac gtatttgtat ttccatccgt taagaatgtg ttttgcgtcc
     2221 aacgatccgc atgtctgacc aaggctaaac ttttgacctt ctctgattgt tcaaagtcct
     2281 tattatcaaa gtaagcccat ccacattttt cataaagaaa gctagagatt ttatcactta
     2341 cttgagtaag tgcagaagga tccaacagac cagacaaaga aataaacttc gtaatctgct
     2401 tgttctccaa agtcggatca agatgatcag cttttttaat ttcttcattt tgaagtgcta
     2461 acaaatattc tccactaatt tgtgcacctg ccgaatttcc accaatcgaa atattcgacc
     2521 aatctgcttg atcttttatc tcttttttag ctgcttgaat ggcttcgttc aattggataa
     2581 ctggtgtagg atgttttgct tccggtgatc gttcataatc tatatttacg atgatttgct
     2641 gcgtgtctga acatagatat tccaaataat cacgacaatc atttttatcc cctcctacat
     2701 atgcgcctcc atgcatccag aaaagaatcg gtaatttttt cttttccact ttcggtgtgt
     2761 agatatccat attattattt ttgtactttg aaggatagga tatttctttt tttatatcga
     2821 tttgttcgat tttttgctgg aaatcatctg gtcgggcata tacttgttct ttttcacttg
     2881 tgtcaaattg ttttctaaca agcaagctaa tcggctttgg tgtgatttga ttaatcaaaa
     2941 atacagtgac taaaataact acgatgataa ctggtactgc aatcactatt cgcttgaacc
     3001 atttcattca tttaccctct ctttcttcgc caaatccatg tgataatcgt gctaacagat
     3061 aaaaatccac ctgaataaat gagaagcgaa tgtttctttt ctccagtttt cggcaacatt
     3121 cttttttttg catctgtctt tttttgtact ttcactgtca tctgaatcgg ttgcgtatca
     3181 tcacttgatt gatcctctcg aggttcattc tttcccttat tgtctgcatt ctctgtcgga
     3241 tcttgctttt ttggtggagc tactgggatt tttttgtaat agaaaataat agttggtatc
     3301 gtctcattaa aaatcccttc agtcggtcct tcaacatttg ctaattcgta tccttcgaat
     3361 gtcttttgct ctgtcatata attttctcca taagctccgt aaagctggat ttcgtcctgg
     3421 tctttgagta gacttttatt gttttcatcc acatacttta cgtttatctg atcatggaac
     3481 actactttca aaaagtccgt tacatgtttc gtgtaggcag catggtcaac ttcctgtgcc
     3541 tttccatgcc ctgctccttc tactaataaa agatctttat accctcctga taactcatat
     3601 aatttttgcg cgttagacac aggtactaca tcatctgctg ttccatggat aatcaattta
     3661 ggcaatgaat ctgacattcc aatcgtaggt agagcagctt ttgtatccaa atgcatctcc
     3721 tgatcaaaat agtattgttt caaaaggccc ataaataaat cttcatgctc ttgatcccca
     3781 atgatgtcta actgattttt tatggcatcc ggtagtgcgg gggctactgt ctggctgtac
     3841 gtaaaacgta gttgttcatc aattgatgca aatccgcaat tttcaataat tcctttgaca
     3901 ttgacaggaa tatctttcgc taatacagac atcatcgtag ctgctcccat cgaaccacca
     3961 taaagtaaaa tcttttgaga tggccgctct cgaacttctt gatttatcca gtaaaggacg
     4021 tcatctgact catattgtcc ataggtcaca taatcgcctc cgctattacc agtcgctctg
     4081 gaatcaacaa ataataaatt atacccggcc ttataaaaat cattgtattc ttctgtaacg
     4141 atcccgttat tccaattccc tcgaaatccg ccatggatca tgactgtttt atctgaacct
     4201 tgatcgacat aatatgcata tacatccatc attttgccat cttgtttgtt tagtacagtt
     4261 tgatgaagtt gttttgtatt tggatagaag cctgtgtttt ctgtaggcgt tgtcccatca
     4321 tctgtacctc caagcatcga catcaacaca ctgttttttg caaaagcgat atcgtacaat
     4381 agataagtag caatcagatg tttgataatc tctttatcta caccataatc attaacagca
     4441 agctcagtga ctaataactc tattaatttt gacttgatgt cttctgttgg tccattaagt
     4501 atttctgtca tctgtttacc gaagtaattc aaatattctt taccattgaa attttcacct
     4561 ttaccgatat catcattttc tgtcccattc tctttgtcac ttactttagc agaagaggat
     4621 tcgtcgtttg acatttttgt agctgttgct tcttctgttt gttggttatc tgtggtagcg
     4681 gtcgtttctt gatctgtgct agtaacagat gtctcaaccg ttgtttcagt atttgtttct
     4741 gtttgtgaag tactcgattt cgtttcattt ccagcatcag tagtttctgt ggtttctacc
     4801 tcccgagatt ccgattcgga agaaatgctc tctccatata ctgtccattg cgggacgctt
     4861 gtacttacag caaataagat gaccaacgct ccctttaata cttgttttgt tctcaagcta
     4921 ttcactcctt tttatgtttc taggagaatt atatgtaaac gcaaacattt tttatacaaa
     4981 aaataaatgg tttatcccat aaaaatagaa caggattatt ttctcatttt acgtaataaa
     5041 gcagaatgga taaaccagta tccattctgc tttttttaaa cgaagaaaca acttattgct
     5101 ccaaaaatgt cagaaatcgt ttatcccaaa ttttctcgta aaaagcgatt gtttcctttg
     5161 ctttcataaa aggattatca taagttgtcg ttgctcctct acacatcata agctggtatc
     5221 ctaatccatg agcaaacttc acagtcgtat ctatacaata ctctgcttgt gccccacata
     5281 attcgatcga atgacagtcc aattcagtta ataaaacact caaattagtc ttataaaatg
     5341 aattcgcatg ggtttttctc acaaaataac tgtgctcagg aatagataat tccgacaata
     5401 gctcccatgg ttttgtttgt ggcactaatt gagcatcttc gtgttgaacg aaaataactg
     5461 gtttcccaat cgtttcgtat gttttgattc ttttattcac taatgctgtc actgtttcta
     5521 attcgtgtat cggcgaagaa tcaaaacata cccctttttg taaatcgatc actactattg
     5581 cgtcggctgt gtttgacata cttgtgcctt ctttcttttt tcttgattta atagtatttc
     5641 ttatctagct agttccatta tacgtgacaa cattttctta ttttacacct ctaaatggca
     5701 cttttcgact atttcttcat gttattattt cataaatact tagctattgg aggaatgtat
     5761 gatgattaaa ttgtatacag gttctggctg ctcgtcttca agacgcgcaa aacgttggct
     5821 tatcgtccat gatataccgt ttatcgaaca gaacttaatt cgtcaaccac taaccaaaaa
     5881 tgaatttatc cacttactaa aattgacaaa caacggcgtg accgaaatgc tctctactca
     5941 ttctattagt tataaaaatc ttgttcacag aatagatgaa ctcagtttga gtgaactata
     6001 tattgagatt cgtacatccc cttctttatt gaaaaaaccg attatctacg accaatctgc
     6061 aaaacttttg actggattta gcgaagataa aatcaggatg ttcctttcta aaactaaaag
     6121 aaagcaagag ctcaacgaac tcttgctttc tggtcatctt gtcgatttta aagaaaataa
     6181 tctttttgca atgtcttaat tttggcataa acaatctgct tttcttaatt aacgaaatcc
     6241 aatttttcca atatggatct atttttctca ttcctgattt ttatgatttc agctttcaga
     6301 cgcgcccaat cattttttgt aggttgtcca ttccagtaaa atatctgtgt attcgtttga
     6361 tcatatgcct ttttgtcgat aggataatcg ctaatgacaa tatcaggtaa ctgatcagcg
     6421 gatgcactat attttattgg gcgttcagtc atagataata tccttttctt tgcccataat
     6481 tcctgtgttt taccatactt tgattgaata tagaatatga caggttgttc gtgttttata
     6541 aaaatatccc tgactaacat acagtattga aaaatcaacc gtctgttctt ttcataataa
     6601 gtattaagct ctggaattct gcgagctacc ccactaaaaa actgttgcat gattggatac
     6661 atcactggaa atatatgctg aaaatctctg tcttctgctg attttccaaa agcatccacc
     6721 ttcttttttg tcccaaaaac ccttgaatac aaatggatca ttgccaaatt gacatataga
     6781 taattttttt ctgaagttgt aaaacaaatc ttgaagtttt tttcaaaatt ttctacaata
     6841 gacgattcaa tccagcccca actttcttgt actttatctg gagtatccaa caaatcttct
     6901 tccaaatagg tgttcattac accaaacata aaataaaggt aagcttgctc tgttggagtg
     6961 atgtgattca tcgaccagaa gtttgcttca aaaaaaggct ctagtatgta atgcgtaaac
     7021 tcttcttttg ttatcaatgg atgcacaaat ttgttcatct cttctgggac atacgtaatc
     7081 gagtggcctt gtttcattcg ttgcaacgaa attcttagta ggctgatcca tttctttcta
     7141 tctatactac gcgtatatgg cgcatgtttc gcaaattttt ctaatagttg acgtttattt
     7201 tttatacttg ataatatatc agaatgattt tcgtcgaatg actgccaata catcaagtga
     7261 aaaaagtatc ggatacggta ttcttctcct tctatcggat tacctttttt caagctgcaa
     7321 gataaatgat agtttttcaa aatagtatta agttctctta attttctttg aatcgtacgc
     7381 aaacttatat aattttttga ctaagatttt ctaaggattg ataagtacct ttgtaaagtg
     7441 aatccagtaa aatataacca atcgttccct ctaaaagtat agaataaact ttactcagta
     7501 aaaactgaga agaaaactcg acttttatca tcttgttttc tgaaagaaaa gataatttat
     7561 attctggata cctttcgcaa aacgattctg cttgttgaat caccgaattc atcttagcca
     7621 gggtgtagtt tagatctaaa gcgatatcct tcaccacata taatttctcg tctctttcat
     7681 aacaatagtt caaaaattga tatacttgcc tttcattgat atcaataaat tcttcaaaca
     7741 aagtacatac tcctttttat ctattattta gacttattcc gacagctaca tgaataacgt
     7801 acaaatagat tcgttactga tttttcagag atactaccat gcgcagtgtg cattgtcaat
     7861 tattcttgct cttattttcc atttgtgttc ctgttatttc ctgtttctca aatatgttag
     7921 taaacaaaaa tagatctggc aaagctacaa cttcgccagg caatacagca ctctcgaatg
     7981 aaatattcac tcaaaaatct atcgttttat tcaattttag atctgttgct tttattcttt
     8041 atttcactcg atggaagaga gaacgaaaat agcttgctcc tacaatcaaa atgagtaaca
     8101 cacccatcca tgtaattagc ttgttcaagg ttctactacc agtttctggt aaatcgcttt
     8161 ttctattatc tgtttctttc gctttttctt gatgatcaat tccacgagaa acaaaatcag
     8221 gctgttttag cacacttttt aggttgctcc ctgcatttct cttttgttcc ttagttggta
     8281 tgataggtac caaatcaagt tcattattta cttttcctgt gctgctttcg ctgctcgtgc
     8341 ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc gtgttttctg
     8401 tgctactttc actgctcgta ctctctgtac tgctttcacc gctcgtgttt tctgtactgc
     8461 tttcactact tgtgctatcc gtactgcttg gcggtccaac aactgtccaa ctgcttggtt
     8521 tcaaattctc cgtgatcgtc atctgtccat cataaaaaac aatcaaatca actttatctt
     8581 tactcttgat ctcatcaatc gatagttctg cagaaatcat tattgtatac tcaataccag
     8641 cggtccattg tacgtctttt aaataaactt ccccaataac tttttctgaa cttccattac
     8701 catcccaacc atatgttaga acagcatgta catcactagc attcacgctt ttgtttggtt
     8761 tgactggaat agtaatgact tgttgtgtca tgtcataaac aatccgatca gttttcacag
     8821 taaatgaaag attctcattt gcccgttcat ttgcagaagt actgattcca atcattgctg
     8881 caattatact cagcccaaga ataacaaata atacggtcgc tgttttcttc atttctatca
     8941 ctctccatat aattaccttc tatttgataa aggaattata taaaatgaaa atgatgagaa
     9001 cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg aatttgttta
     9061 ctttttcgtt taatcaatga cagacttaaa aaagcaagac cgatatcagc cttgctttct
     9121 tcatctttta ttgataaaca gcagtgtatt tcttcatccg ctttgtttta tatgcataga
     9181 gtaacaaacc aacaatcaag actaacaaga ataatgtagt tgatatagat gcttcagccc
     9241 caaagtttcc cccagtaatt attgtatttt gcgattggat cagagtggag aaaaaacctt
     9301 gttcttgcgt tccgctgact ggtataccca aaatagctcc ttgtgtccga ttccaagtaa
     9361 tatgagcacc aatagccgta aataaacttc ctgtactgta gataagtgcg ctgaaaaagc
     9421 aacctgctaa aaagatattg aaaactgcca gaaatgtcat gttaggattc aatccgtgca
     9481 aaagagcaaa taatgcagca gatccaatca ttgctattaa cggaccaaat ctaagcatct
     9541 gatgctgaaa aaagccacgg aataaaagct cttctgatgc ccccactgca ataaataaaa
     9601 taaaactcgt tatcagcggc acaatgtttg cgtttcctga atctaaccga tacgcccccg
     9661 tcaaatataa tggactagta ataagcagaa acactccaat tccaacaacg agacctcccg
     9721 ctaaataccc ccagtctttt cctcgaacag aaacaccata atcttctatt ctttttcctt
     9781 cgaccttact agcaagaaat aaagctagta aaaaaacagc taaatgtccg accgtaccgg
     9841 caattgctcc aatccattca ctatttccaa taaacatatc cgcaagtgca ttattgacaa
     9901 gaattcctaa aacaaaacca actaagacta ttaaaacaga tccaatcaac cgaataatcc
     9961 tgatcatctt tcatccctcc tttatattaa gtttaacaac ccttttcttt cttgtctcgt
    10021 gaaaactatg taaatgttct ccatcataga gagaatagat ttttacgaac taagaatatc
    10081 tacagcaacc ttacgaaaat gaaagactgt ctccctaatt cttctgttag actgttttta
    10141 ataaggaaag gatgggaaat tttttgaaaa aacaactact aattatgtgc ctttcagcat
    10201 gtgcaatttt actattctca ggttgtagca agtccaatga aacgattgca acgtttaaag
    10261 gtggaacact tacagttcag gatttttatg ataacccaaa agttaaatca aataatcaaa
    10321 cactattaaa aaaaatgatt gtcttcaaag catttgatga tgtatacgga aaagagattt
    10381 cttcaaagga agtaacaaaa gaatataatg aacaaatcaa aaaactcggt ccaaactaca
    10441 aggaacaatt aaaagcagtt ggccaaacag aagagacata taagaaactc tttaaacaga
    10501 tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt aacagataaa gatctagaca
    10561 ctgcttggaa agaattctat ccagaagtta gtacacagat cattctattt tcgactaaag
    10621 aaggagcaga taaagcaaaa aaagaagcaa atgaaggaga aaatttctcc aagcttgttc
    10681 aagcttatgg aaaaaataaa acattaaagg aaactgatgg aaaaatgaat tttgattcaa
    10741 ccaatccaga gattcattcc tactgaagta aaaaaagcag catttaaact aaaaaacggg
    10801 gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag ctattatcta
    10861 gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa atctgagcta
    10921 gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa aacaatccgt
    10981 aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa tatattcaaa
    11041 taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt ttggaaattc
    11101 cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt tttttatttg
    11161 taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa aaagtttttt
    11221 atttctatca catgaatcaa tgcgctatta agtagattct ttcatgcttc cctatttttt
    11281 cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg aaaagttatt
    11341 ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt atactagaga
    11401 aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt tattcaaaac
    11461 tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc gtttaaatga
    11521 gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata ttgaatcgtc
    11581 agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa attgattcag
    11641 aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt ttgcacccaa
    11701 tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac ttgatctagt
    11761 agctctttac ctaacgttat cgcatgagct ccagatcgaa tggctgtagt gacttgttca
    11821 caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc gatctcagtc
    11881 cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct gttataataa
    11941 ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc tgtgtaaata
    12001 gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg aaggcctgct
    12061 tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat aactcgagca
    12121 tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg aatctgtccg
    12181 attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc ttttttcaga
    12241 attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga ataaatactt
    12301 tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct gatcctcttg
    12361 atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt ctacatacat
    12421 tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat gtcctaattc
    12481 attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt gtttatcaaa
    12541 acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa tcttataaca
    12601 ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt tttctttcct
    12661 gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg tcatctct
//
LOCUS       DAZUKD010000077        12550 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000077 DAZUKD010000000
VERSION     DAZUKD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.078"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..3219
                     /gene="fss3"
                     /locus_tag="ACG8N9_001908"
     CDS             31..3219
                     /gene="fss3"
                     /locus_tag="ACG8N9_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4897474.1"
                     /translation="MLSLAVQAIVGAIAPTITFADEITHPQTVTVELDLAHQYAVEGT
                     FSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKNPLPDMSDKAKLVS
                     VLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIDDYQK
                     KPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVNGNQLIITPNANSK
                     ESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVETEGSLKIKKVDKE
                     SGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTT
                     PMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSLAGNTFAIRKDSPT
                     GEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELKYANQT
                     VAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTLFTAKDGKAVKWSE
                     AFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAPEGYSLDETKYPVS
                     IKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGFNDLTFKVSPLEGT
                     NEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFK
                     ENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNRLMLYDLPEEEDSL
                     TSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDVDATKAAQEKDEKA
                     KPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYENKEAFEAGDKPVA
                     KDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFDDVSITHDVLDGSK
                     EAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTFAE
                     INYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVPSGSQESSPTVKTF
                     PQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3332..3646
                     /locus_tag="ACG8N9_001909"
     CDS             3332..3646
                     /locus_tag="ACG8N9_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4897475.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3659..4033
                     /locus_tag="ACG8N9_001910"
     CDS             3659..4033
                     /locus_tag="ACG8N9_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4897476.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4043..4378
                     /locus_tag="ACG8N9_001911"
     CDS             4043..4378
                     /locus_tag="ACG8N9_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4897477.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4461..5801
                     /locus_tag="ACG8N9_001912"
     CDS             4461..5801
                     /locus_tag="ACG8N9_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4897478.1"
                     /translation="MYKGHRIRAGDQHLVYHFVIGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5879..6553
                     /locus_tag="ACG8N9_001913"
     CDS             5879..6553
                     /locus_tag="ACG8N9_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4897479.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6546..6710
                     /locus_tag="ACG8N9_001914"
     CDS             6546..6710
                     /locus_tag="ACG8N9_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897480.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6786..7928
                     /gene="dcm"
                     /locus_tag="ACG8N9_001915"
                     /pseudo
     CDS             6786..7928
                     /gene="dcm"
                     /locus_tag="ACG8N9_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7918..8208
                     /locus_tag="ACG8N9_001916"
     CDS             7918..8208
                     /locus_tag="ACG8N9_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897481.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8467..9651
                     /gene="mobT"
                     /locus_tag="ACG8N9_001917"
     CDS             8467..9651
                     /gene="mobT"
                     /locus_tag="ACG8N9_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4897482.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9648..9785
                     /locus_tag="ACG8N9_001918"
     CDS             9648..9785
                     /locus_tag="ACG8N9_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4897483.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            10531..12441
                     /gene="ltrA"
                     /locus_tag="ACG8N9_001919"
     CDS             10531..12441
                     /gene="ltrA"
                     /locus_tag="ACG8N9_001919"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4897484.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4311 a         2085 c         2792 g         3362 t
ORIGIN      
        1 aaacaaaaaa agaaattatg tgtattaact attttgagtt tggctgttca agcaattgtt
       61 ggagcaattg ctcctacgat tacttttgct gatgaaatta cccatccaca aacggtaacc
      121 gtagaactag atttagcaca tcaatatgct gttgaaggaa cttttagtga tggtcgtcca
      181 atgtcagaag tcactgttcc gcattatgca gtgtacaacg gctttaaaca agatattttc
      241 tgtattgaac caggagtccc gatttacaat gagttcactc ctggctatga gaaaaacccg
      301 ttaccagata tgtccgataa agccaaatta gtttccgttt tatggaaaaa agtaggtaca
      361 gatgcagata ctcatattgt ggcacagaag atgatctggc aagaagttaa tgggtatacc
      421 cttcactcaa taaaacgatc agatggtagt gccgtaaata tcgcagccat tgaggcaaaa
      481 ataaatcaag ccattgacga ttatcagaag aaaccaacct tccataatag tacggctaaa
      541 acagtattag ggcaatcgac tactgtgacg gatacgaata atctgaattt gtcagagttc
      601 gatgaggttg tggaaaatgc cgcaaacatt gattatcgtg tgaatggtaa tcagttgata
      661 atcaccccaa atgccaattc aaaagaaagt ggtgtgctga cgcttaagaa atctgctggt
      721 accggaacac ctgtcgccta taaaaaggct ggccaacaga ctttaatggc cggggcgatt
      781 gataaaccta atacctacac aatcaaaatt gatgtggaaa cggagggttc tttaaagatc
      841 aaaaaagtcg ataaagaatc gggtgatgtt gtaccaggaa cggttttcca tttagacttt
      901 ggaaaaactt tacctgcaaa agacgtgact actgacaaag aaggcattgc tacattggat
      961 gagattcctc atggtacaaa agtaaccatt accgaaaaat cagtaccggc gccttatacg
     1021 attgatacca cccccatgac taccaccatt aaagcaggcg aaaccattta cgtgacttca
     1081 aaaaacgcac gggaaaaagg gcaaatcatt ctggataaaa gcggagttga aacagggagt
     1141 gatctttgga atgacaatta ttccttagct ggtaatacgt ttgccattcg caaagacagt
     1201 ccaaccggtg aaatcgttca agaaatgacc acggatgaaa acggtcatgc ggaaacacca
     1261 aaagagattg ctaatgcgtt ggaattggga acctattatg tgaccgaaac taaagctagt
     1321 catgggttcg tgaatacgtt caaacctgta aaagtcgaat tgaagtatgc caatcaaacc
     1381 gtagcccctg ttaccagtaa cgtaaaaggg caaaatcaag aagtcactgg cgaaaccact
     1441 ttgacgaaag aagacaaaga tactggggat aaagcacaag gtaaggcagt ctttgagggt
     1501 gctgaataca ccttgtttac tgctaaggat ggaaaagctg ttaaatggag tgaggctttt
     1561 aaaccagagt tagcgaaggg aacgaaaggt tctgatgaaa cggtcacttt gactttagac
     1621 gaaaagaacc aagttgccgt taaacattta gctatcaacg agtattattg ccaagaaaca
     1681 aaagcgcccg aaggatatag ccttgatgaa acaaagtatc ctgtatccat caaaaaggtg
     1741 gatgatagcg aaaaaaatgc cgtgattacc cgaaatgtta cggcaaaaga acaagtgatt
     1801 cgctttggct ttgatttctt taaatttgct ggatcagctg cgggcactac cgaaactggc
     1861 ttcaatgatc tgacctttaa agtgtcgcca ttggaaggga ccaatgaaat cacaggtgcc
     1921 gaagatgaag cgaccacagc ttataacgag caattagggt ttgatggtta tggcaagttt
     1981 gaaaatcttc cttatgggga ttatttactt gaagaagtag aagccccaga aggttttcaa
     2041 aagattacgc cattagaaat ccgttctact tttaaggaaa acaaagaaga ctttgtgaag
     2101 agtgagtatg tctttaccat taccgaacaa gaccaaaaac aaccaattaa aacggtgacc
     2161 gttccttacg agaagctgac gaacaaagca ttttctgtta gtttgaaccg tttaatgctc
     2221 tatgatttgc ctgaggaaga agatagttta acttctcttg cgacttggaa agacggaaac
     2281 aaagaattaa cttctctgga ttctaccgag ctagttgata aattgagtta caatctacat
     2341 gaaatcaaag aagactggta tgtggtagcg caagccattg atgtggatgc gacaaaagct
     2401 gcccaagaaa aagatgaaaa agccaaacca gtggtgattg cagaaacaag cgcaacgttg
     2461 gcgaacaaag agaaaacagg aacttggaaa attcagcata aattaaccgc cgaacaagtt
     2521 ttaaataaaa ctattgtctt attcaactat gtgtatgaaa ataaggaagc ttttgaagca
     2581 ggcgataagc cagtagcgaa ggatgttagc ttgaataacc aagcccaaac tgtcagttgt
     2641 acagtggaac accatgtttc tatccaaaca aaggcccacc tagaaaacgg ttctcagacc
     2701 tttactcatg gtgatgtggt ggatatgttt gatgatgtgt cgattactca tgatgtactg
     2761 gatggctcaa aagaagcttt cgaaacaatt ctgtatgctt tattaccgga tggtacaaac
     2821 aaagaaattt ggaaatctgg caagattgat tatgaagtaa atgataaaga attcaccaaa
     2881 actgtgcttg cgaaaaaagt agataccgga aaatatccag aaggaaccaa gtttactttt
     2941 gcggaaatca attacgataa agatgggaat atcaatggaa aacacaatga agatttgaaa
     3001 gaaaaatctc aaaccttaac accaaaagaa gttccaacca tactaagtac gccgaaacaa
     3061 ccggaaacac caactgttcc aagtggctct caagaatcta gtcccacagt gaagaccttc
     3121 cctcaaaccg gggagaaaaa ttccaatgtt ttactgttca ttggttttac tttgattttt
     3181 gcgacggcgg gctattattt ctggaatcgc cggaactaag gtgatgagat gatacaagta
     3241 aatcctaaga accaacgtgc gcgaccgcct cctaaaaaag tagtcgcgct atttttgtat
     3301 ccaaaactaa caatgaaaag aggaaaagaa gatggaattg aaatttgtcg tgccagatat
     3361 ggctgaaacc tttggaaagt taagttatgc cggtgaagga gaaattataa cagaaggata
     3421 tggacggaat actacggtga ttggtcgcag ttatcatttg tattccagta agcaacgggc
     3481 cgatgatatt gaggtggtag tagctgcgga agccggtgaa aaggattttg atcaagacca
     3541 accgctaaaa gccgtgaatc cccatttggt tgccaaaggc tatgagattg agaatcgtgg
     3601 gtttactgat tacgtgttgt atgttgatga tttagtgaaa gcataggagg aaaagaagat
     3661 gagattaaca gaaggtattg tcgtagattc aggtttaaca tttgggaagt tgcgtttttc
     3721 cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga acaattggta atgaggtaaa
     3781 agaacgaacc tataatttaa aatcctctgc acaagggcga atgattcaag ttagtatccc
     3841 agctaatgta cccttacgcg agtttgctta tgatgcagag gtagagttgg tcaatccaat
     3901 cgtggatacc gttgccaatt atgtttttcg agaaggaact accgttaatt ggttcattaa
     3961 ggcggatgat ttagtcttga agcggcagcc gaatcaagga aatcctacga accaaaatga
     4021 aagaaagaaa taggtgaaga ttatggaaat taatgttgaa catattttag attgtctcga
     4081 tcaatatgga aaaggcgagc tgactgagga acaactcgtt aaagcattaa cctctgatga
     4141 aaaaatgttt ctgattatac atcaaggttt acttggagaa aatagcgaca gtgaagaaga
     4201 ctctgatgta ctagactggt taggagaaga agagtctttc tttatagttg tcgaagtaaa
     4261 tgaaaactta tgtcggcagg cagaatccat tttggaagaa attggtgtgg agatgccgga
     4321 tgctattgaa ggctttttaa atcaattggt tgaaacgaaa caactcccct cctcgtagca
     4381 gtaaatgact agcataactt tttgaaattg cctaatgaaa tcctattggg ctttttcgat
     4441 tggaggtgga aaccatcaaa atgtacaaag gacatcgcat acgcgcagga gatcagcact
     4501 tggtttatca ctttgttatt ggctggctac tcgctctgtt catcggttgg atgagcgtct
     4561 tttattttca agagtttaga caatttgata tttctaagct atcactttct actattgaaa
     4621 tcgtttggtc tataaaagat ttagtatgtc tgcttggaag tctcgctttt tcaggagcta
     4681 tgatattgct ttatattcac ttttttcttg atcattggcg aagcttgtgg catcgacaaa
     4741 agctagctcg gatgattcta gagaaccatt ggtatgaagt gaaacaaact caaagtgaag
     4801 gctttttcaa agatttgaat agtagccgaa ccagagagac tatcagttac ttccccaaaa
     4861 tctgttatcg aatgaaagat ggattactgt ctattcgcgt tcaaatttca cttgggaaat
     4921 accaagatca gcttttaaaa ttggaaaaga agttagaaag cggactgtat tgtgagttag
     4981 tagaaaagga actcaaagac tcctatgtgg agtatacttt actgtatgat atgatcgcca
     5041 atcgaattgg gatagacgaa gtagtggcag aaaatgggac tctgcgattg atgaaaaatc
     5101 aagtatgggc ttatgattct ttaccccata tgttaatcgc tggtggcacg ggtggaggga
     5161 agacctattt cctcctcacc attatcgaag cactattgaa gtcagatgca gaattgttta
     5221 tcctcgatcc caaaaatgca gatttagctg atttaggtac ggtgatgcct catgtttatt
     5281 cacaaaagga agaaatttct gcttgtgtgg aagatttcta tgaacgtatg atggctcgta
     5341 gcaaggcaat gaaagaaatg tctaactaca aaacaggaga gaattatgcc tatcttggac
     5401 ttccacccaa ctttttaatc tttgatgaat acgtggctta tatggagatg ttaacgacga
     5461 aagaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttgggt cgtcaatctg
     5521 gtttctttct gattttggct tgccaaaggc cggatgcgaa atatttagga gacggaatcc
     5581 gcgatcaatt caactttcgc gtggctttgg gtcgtatgag tgaactcgga tattccatga
     5641 tgtttgggga ggtcgaaaaa aacttcttta tgaagcacat caaaggtcga ggttatgtgg
     5701 atacaggtgg cagtgtgatt agtgagtttt atacgccact tgtaccaaaa gggtatgatt
     5761 tcttagattc aattagtaaa ttagaaaaga tgaatgattg agtgatcttc aatactgact
     5821 ttatacttca agtaacgtat aatactttta tatagtagga agaaagggga taattaatat
     5881 ggaaagtaat gattcttatt tagagtatgc aaaagcaaat aaggataagt ttatttctta
     5941 tattatcaaa gggaaatccc ccgaagttgg acgagacgca gtttttatgg cagggagtcc
     6001 tggggctgga aaatcagagg ttgccattgg attagctgag aattatgata accatgtgat
     6061 tattgatgcg gattactttc gcacccagtt tcctgattat aatggaaaaa actctagtct
     6121 ctttcaaaaa gcatcttctt ggttggtaga gcaggcattt agatatgtct tagatcatgg
     6181 ctattctttt attctggatg caacttttgc aatattaagt gctgaaaaaa atatctcgcg
     6241 ggcgttgaaa aaacattttc gagtgacgat tttctatgtt tatcaagacc ctaaaatcgc
     6301 ttggaaattt acacaagaac gtgaaattgc agagggacgt catgtgccga aaaaggcatt
     6361 tattaacgct tttttcaaat caagagagaa cattgcaaaa gtaaagagta aacatccaga
     6421 tgtattgctt catatcataa taaaagatta tacgaataat atttccgaag ttcattatgc
     6481 tacggataat ctagaattat ttttaccact agggtatagt aggaaagatt tggaggttca
     6541 attacatgac tgagaaagaa caagtaacga aaattgtaaa gaaatacaat aagagcattg
     6601 cggatttatc ggaaaatgct actgcaaaag agttcaaaac ggtgattaaa tatgtagccg
     6661 atcaggccaa tgaaaaacaa cggaaattag ttggattaaa taaaaaatag taggacaaat
     6721 taataatgta tgagccaaga gcaaaatgtt cttggctttt ttctttgcaa tgaatgaggt
     6781 aatgtatgag atttttagat ttattcgccg gtattggtgg ctttcgttta ggtatggaac
     6841 aggcgagcca tcattgtatc ggtttttgtg aaattgataa atttgctaga cgtagttata
     6901 aagcaattca tgatacaagt aaggaggtgg aaatgcatga catcacaagt gtatcagatg
     6961 agtttattca atccctcgga ccagtggaca tcctttgcgg cggatttccg tgccaagctt
     7021 tttcaattgc gggaaagcgg caaagatttt ccgatactcg aggtacttta ttctttgaaa
     7081 tcgctcggtt caccactctt ctccgaccta agcttttatt ccttgagaac gtccgaggat
     7141 tgctcaatca cgaaggaggg gctacgttcg agacgatcct ccgaacgctg gatggattgg
     7201 ggtatgacgt ggagtggtag gtgcttaact caaaggccta cattccccaa agccgggagc
     7261 ggatattcct tatcggacat tctcgagacg catgtaccga acaagtattt cctatcattg
     7321 gaccgtcttc aacatctgat caaaacatca gaaatttggt aaatatcaat ccttccaatc
     7381 gaggaatggg tggacaagtc tatggttcag atggcgtagc gccaacctta acaggtgatg
     7441 aaggaatcaa aatcgcactg ccagcaaggg atggaatctc tgtagcagga ctattaccgg
     7501 gaaagtttga acaaggtaat cgagtttatg aagttactgg aaccgccccc accttgtcaa
     7561 cgaaacaagg tggtacaaaa atcatgattc gaaaaaatga aacagaatat caagaggtga
     7621 agccagaaga cagtgtgaat ctggcttttc ctaattccac aagtcgccga ggtcgtctgg
     7681 gaaaacagag tgttcatacc ttactaacag gagaccaaca agcgatcgtc acggatcagt
     7741 atcaaattcg gaagctaaca cctagagagt gttggcgact tcagggcttt cccgattggg
     7801 cttttgatcg agattctcaa gtcaattcag atagtcagtt gtataagcaa gccggaaact
     7861 ctgtaacagt tccggtcatt tttgatattg ccagaagatt aaaagagttg gatgtacatg
     7921 gtttatgatg agttgattgg agaaatttat tgggtgattg gaaagattca gtcagaccca
     7981 gaattagaag aacaattgcg acggttaaaa tttgatattc gcaagaatgg tgtcaaagtc
     8041 gctggcgatc catatttaat gaatgaggag acggatgctc gaattgaaat aaatcaagta
     8101 attgcagaat ttgagcggat tgcggaccaa gcaaaagaac cagatattcg tcaatacttg
     8161 ttagaaatga aagcagagtt ggaagtaaat ggtattacag atgagtaacc taaaccaaca
     8221 caactgactg ttaggcgctg accgcggagg attcgcgaag cagaaatcct ccgtggtgct
     8281 gtgcctgttc atggacggcg ttagccgacc atgatgtctc acccccgtat ctaacagggg
     8341 ggtacaaaaa cacaaaaaat aagtaagcaa ccattaagaa tgcttgttgt atcaaagata
     8401 gtaagtaatt tgtataggtg ttaactcatt tgaaaaagtt aacaccttga atttggaggt
     8461 gagaacttgg cacaacggaa tttagattat cggctattaa aagaccgacg caacgaatac
     8521 ggcgtttcac aaaataaatt ggctacggct tgtggactta gtcggccgta cttaaatcag
     8581 atagaaaatg gtggcgtaac cgcatctata aaaaccatga ggaaaatttt tgaccaactg
     8641 gaaagcttta atcccgattt gcctttgacg ttactgtttg actatgtaag gattcgcttt
     8701 cccacaacgg atgcacgaaa aattattcaa gaaattctcc atttgaaatt tgagtatatg
     8761 ctccatgaag attatgcctt ttactcctat caagaacagt atgtcatggg tgatattgtg
     8821 gtgatgttgt ctcatgaaga agataaaggt gtgcttttag aattaaaggg gcgaggttgc
     8881 cgacaatttg aaactttttt actcgctcaa aagcgtagct ggtacgactt tttcgaggat
     8941 tgtctaaagg ccggtggcgt gatgaaacgc ttggacttgg caatcaatga tcgagtgggg
     9001 ctattggata tacctgagct aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
     9061 cgtaccttta aaagttatcg ttccggggaa cttttaaagg ctgatgaaaa ggacggcatg
     9121 ggcaatacct tatatatcgg cagtctcaag agtgaggttt atttctgttt gtatgaaaaa
     9181 gactatgaac aatatatcaa gttagggatt ccattagacc aaactgagac gaaaaatcgt
     9241 tttgagattc gactgaaaaa tgaccgtgct tttcatgcga ttcaagattt gttaaaaggt
     9301 cgtagcattg agagcaccac gttttccatt atcaatcgtt acttgcgatt tgcggataaa
     9361 gtagaaggta aaaggcgaac caactggccc ttgaatgaac aatgggggcg ttttattgga
     9421 cggaatcgga aagagattca attaacttcc gagccaaaac cgtataccat tgaacgaacc
     9481 ttgaattggc taggccgaca agtcgcccct acgtggaaaa tggctaagga gttggatcga
     9541 ttgaatcgga caacctatat ccaggatatg gtacagaatg cacgcttatc agaccgacat
     9601 aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa
     9661 tcgtcatggg aattgtaaaa gatatttttt tggtatttgg tggcagtatg cttggtgtta
     9721 ctgtgatgtg cttgatgaat gcaagtgcag cagcggatcg agcaatggaa aagaaaggaa
     9781 agtgacaaga tgaatttcgg acaaaattta tgtgcgactc gttccttagt gaaaagctaa
     9841 ggcactaata aaagaacgaa aaagtttggt tattaggaga actgataatc tgacaggtgg
     9901 aatgatgaag taacgctctg aaacgcctgc cctgagcctc cgacaagcat tgtatgagga
     9961 aactcagaat gtttgaagct cggtaaagac ggctgaaaga taccgtaata gttgatgaac
    10021 atatatcttc tatcgaaaac tatccagagg tgggtagcgt atccaacagg tcgggggtct
    10081 ataaaatatc tatggttaga atgttctata aggaactgac gaaacagcga atgtacgggt
    10141 ctaaatttga actttgtaga aatgcaaagg tacgagaaat cgtagctggt gtggcaaagt
    10201 aagaatatag gttatgaaat gccatataac gttacaggcg gtatcaagcc agcaggctta
    10261 tagctagtga cctaaggata tatatgtata gataagatta tcggaacgag gaaaggtatt
    10321 gggttctcat caagagaata aactgacgaa gaacaataag cagttgaact aatgctgaaa
    10381 agcagagggc gaacctaaga aattcttgta atgagaatgg aggaatgccc tcaagtcgtg
    10441 tatatattag aaaagtatta tgttgtttca ataaggatta cgagtaagat aaaaaagctc
    10501 actcaatttc aagtaaagga gtgaagcctt atgcctaaaa ataaaaatga aacattgtgt
    10561 gtagaagatt taagacacgc tgaatattac gaaatgcaaa acacctttga tgatttatat
    10621 gccaaaagta agaatggaga tattttcact catcttatgg atattatcct atcgagagaa
    10681 aatatattac tggcatatcg aaatatcaaa gcaaatgcag gcagtaaaac agcaggaaca
    10741 gacggtacaa taattaaaga tattggcaaa ttgccagcag aaacagttgt caaaaaagta
    10801 agatacattg ttgcaggcag tccacatggg tatcgaccta agccagtgag gcgaaaagaa
    10861 ataccaaaac caaatggtaa aaccagacct ctaggtattc cttgtatgtg ggacaggctt
    10921 atacaacaat gtataaaaca agtattagaa ccaatctgcg aagctaaatt tagtgaaaac
    10981 agctatggtt ttagacctaa taggtcagtt gaaaatgcaa taaaagcaac ctataaccgc
    11041 ctacaaatca gccagctaca ttatgtgatt gaatttgata ttaagggttt ctttgacaac
    11101 gtgaaccatt ccaagctgat aaaacagata tgggctatgg gaatacggga caagcacttg
    11161 atattcattt taaaaaggat attaaaagct ccaatcaaaa tgactaacgg tacaataaca
    11221 tatccagaga aaggaactcc gcaaggggga ataatatccc cactactagc aaacattgtt
    11281 cttaatgagt tagaccattg ggtagaaagc caatggcagg aaaatcctgt tacaaaaaac
    11341 tatgtggttc atatcaataa aagcggtagc ccatgtaaaa gcaacgctta caaagaaatg
    11401 aagaaaacaa agttaaagga aatgtatatg gttcgttatg cagatgattt tagagttttc
    11461 tgccgctaca aggaaagtgc tgaaaaagcc aaaatagcaa tcactcaatg gattgaacaa
    11521 cgattaaaat tggaagtatc acaagaaaaa acaagaattg taaatgttag aaaaagatac
    11581 tccgactttc ttggtttcaa gattaaaatg ataccgagaa gaaagaagtt agttgttaaa
    11641 tcacatataa gtgataaaca attcaaaaac caaaaagaca agctggtatc acaggcaaag
    11701 aaaattgctt ctccctcaaa agggaaaacg gaattagaag aaacaagact ttataattca
    11761 atggtattag gtatgcaaaa ttattatcag attgcaacga atgtaaatct ggattgtatg
    11821 aaactgaata gagctgtaat ggctatcttc actaacagat taggtacaaa caaaaggggc
    11881 agattgagaa aaacaggcag acaacttacg acaaaggagg caacaagata tggtaaatct
    11941 aaaatgcttc ggtatgttgc tggcgagggc gaacctattt tccctgttgg atatatccaa
    12001 cacagaaaac caatggcaaa ggtgtattta gcgaattgct acactctaga gggaagaaaa
    12061 tttatccaca ccaatctaag tgttgataaa ttactgatgt accagttaat gaaattatct
    12121 ttggaaaatc gcacaataga atacgcagac aatcgaattt cgctattttc tgctcaacat
    12181 ggaaaatgtg ctataactct ggaagaattt caacaagcta atgaaataca ttgtcatcat
    12241 attgtaccaa ctggagctgg tggaaatgac gattataaaa atcttatttt ggtaaaagag
    12301 gcggtacacc gtctaataca cgctaaaaca gaagaaacaa tacaaaaata tatagctcta
    12361 ttgaagctaa ataaagtcca gctcataaga ctaaataaat atagagtatt agctggcaat
    12421 caagaattaa acttgatata ggtctactac ataaatactt ttcaatataa acacgatgga
    12481 acgcggagtg cggtgaaagt cgcacgctcc gtgtgaatgg ggggaaaagt ttgagatatt
    12541 tataatatca
//
LOCUS       DAZUKD010000078        12520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000078 DAZUKD010000000
VERSION     DAZUKD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.079"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1949
                     /locus_tag="ACG8N9_001920"
     CDS             66..1949
                     /locus_tag="ACG8N9_001920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4897485.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2024..2308)
                     /locus_tag="ACG8N9_001921"
     CDS             complement(2024..2308)
                     /locus_tag="ACG8N9_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4897486.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2298..3032)
                     /locus_tag="ACG8N9_001922"
     CDS             complement(2298..3032)
                     /locus_tag="ACG8N9_001922"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4897487.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3161..3784
                     /locus_tag="ACG8N9_001923"
     CDS             3161..3784
                     /locus_tag="ACG8N9_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4897488.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3976..5097
                     /locus_tag="ACG8N9_001924"
     CDS             3976..5097
                     /locus_tag="ACG8N9_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4897489.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5104..8232
                     /locus_tag="ACG8N9_001925"
     CDS             5104..8232
                     /locus_tag="ACG8N9_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4897490.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLNLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8251..11445
                     /locus_tag="ACG8N9_001926"
     CDS             8251..11445
                     /locus_tag="ACG8N9_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4897491.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFHQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11591..12298)
                     /locus_tag="ACG8N9_001927"
     CDS             complement(11591..12298)
                     /locus_tag="ACG8N9_001927"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4897492.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4266 a         2209 c         2639 g         3406 t
ORIGIN      
        1 tgctattttc acttaatacc cgtatattac gaatgaaaaa agtttatgaa aagttggaat
       61 attgaatgga aaaacaaaaa aggttaaaaa acagccgcta catcactggg tttgatgggc
      121 tccgttcatt agctgtggtg ggtgttatct tctatcacct tctgccaact agtatgaaag
      181 gcggctattt gggcgttccg atttttttcg tcgtatctgg ttatttgatc acggatctgc
      241 tgcggcagga atgggaacaa aatggaaaaa tcaatatttg gcaattttat gtcagaagaa
      301 tgaagagact ctatccggca cttgttttta tgcttgtgac agcttcggct tatatcactt
      361 tgtttcagcg agggctgtta aacaatcttc gaggagttgt ggtcagcagc ttactgtatg
      421 tcaacaactg gtggcaaatc aataacggtt tatcttattt cgatcgattt gctaatgagt
      481 caccgtttac gcatatctgg tctttagcag tagaaggtca aaactatttg gtttggccga
      541 tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa atggatcttt tatactgtgt
      601 taggtacatc cttcctctcc gccatcttga tgatgatctt gtatacacca ggaggcgatc
      661 caacccgtgt ttattacggt accgacactc gtctgttttc tatatggttg ggaagtgcgc
      721 ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata
      781 ttctgaatat cgctggagga atctcgcttt tggcactgat tctttgcttc ttctttttag
      841 atgatcatta caatttcatc tattacggag gaatgctcct agtgagtctg ttatgcgtag
      901 tgctagttgc agtgacagca caccccggag caagtttgaa tcgatggctg acaaatccag
      961 tatttacttg gattggcaaa cgaagctatg gcatttatct gtatcagttt ccagttatga
     1021 ttttttatga agcaaaggtg actgatattg ccgatcatgt tttgttgcat actttagtgg
     1081 aaatcacatt gattctaatc atcagtgaac tttcttatcg atatgtggag cgtccgctag
     1141 cacgttttga ttatggtcgt acattccaag tgatgaaagg atggttcagc aaaccgatca
     1201 tcagtaagag caaaccatgg gttgttccag gcacattact tgtagcaatt gcactcgttg
     1261 gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg caggcaaaaa
     1321 tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac accactagtt
     1381 cggttgacca agcgatcctt gataagtacg agttgtccaa ggcacaaggg aataaggcac
     1441 aaaaattaga actaacggca tttggtgatt cagttatgtt ggatgctgca acggatctac
     1501 aagaacttta tccaaaagta gtggtagatg gagatgtcgg acgacaatta tatgccagtg
     1561 taccgtacat tgaaaaatta aaggaacaag atttgttgaa agacactgtt ctgattggac
     1621 taggaacgaa tggctcattc tcggaagctc agtttgatag tgtgatggat gcaatcggtg
     1681 atcgaaaagt atactgggtc aatgtccgag taccgacgaa gcgctggcaa aatgacgtga
     1741 atgcgatgct tgaacagatg acgaaaaaat acgacaatat gacgctgatt gattggtaca
     1801 gctacagtaa tgaccatagt gactggtttt atgatgatca agtccatcca aatcctgaag
     1861 ggatgacaca ttacattcac ttagtcagcg aagccttgct aggaggaagt tcaagtacga
     1921 acagcagtga gacaaacgtg acagaatgat agctacaaaa aacttcaggc aaaaacattt
     1981 gtttttgcct gaagtttttt tatatttacc gtgtttttaa cattcatata gattcaggaa
     2041 gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg agttttttga
     2101 atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca tggatcatct
     2161 taacaggtct tcttttttga agacgagtat atttagcacc agtaccattg ttatgttcct
     2221 ctaatcgtct agaaagatcg gttgtatatc caccataaaa tgtctgatct ttacagaata
     2281 atacgtaaaa ataatgttca tttgccatag agcatctcac gaatctctgc agtgtattct
     2341 ccatccatat tgtaagtgat caatggtgga gcgactttga atccgtctaa tttgccttct
     2401 ttgattcctt caactaacaa aatatttgcc tcttttccag gttttgggta aacaaattgg
     2461 atccttttag gtgcaattcg atagtcttcc atgacctgaa tgatttctaa gaaccgatca
     2521 ggacgatgga ccatagtaaa gcgtccattt gtttttagta gcttacttga aatatcgatc
     2581 acttcttcta acgtcgtatg aatctcatga cgggcgattg ccaggtgttt attagggttc
     2641 gttttatttg ttggcagccc tttgaaataa ggtggattac agacgactaa gtcacaagaa
     2701 ttatgtcgaa cgacagataa gctgtctttc agatcgatgg tgtggacagt catttgttca
     2761 tttaaatggt taagcgcgat actacgtctt gccatgtctg ctagacgagg ttgaagttcg
     2821 atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc gccatttcct
     2881 gcacaaaggt caacaatctt ccctttttta ggtacacggg aaaagtgagc aagcagcaca
     2941 gcatctaaag aaaaagaaaa cactttggaa ctttggataa tcttgatatc ttctgcgtat
     3001 aattggtcga ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga aaaaaatagt
     3061 cgtctgtatt atactatttt tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt
     3121 ttatgcatac tatgaccata agaagggaaa ggaagagact atgttttatc gatttatgcg
     3181 cggggtagtt cgagtactat tagccttagt taatggaaat gcccgctatg agaataaaga
     3241 agttttaccg caagatgaga attacatact agttgctccc catcgtactt ggtgggaccc
     3301 attatatctg gcggtagctg cagcacccaa ggaattcagc tttatggcaa aagaagaact
     3361 gttcaaaaat ccagttctgc gatttatatt aactcgatcc aatgcttttc cagttaaaag
     3421 agaaaatcca ggaccaagcg tcattaaaac acctgtaaag attttgaaat cgacaaattt
     3481 gagtttgatc atgtttccca gtgggacaag acatgctaca gaacttaaag gcggaatggc
     3541 attgatttcc agaatgtcta aagcgaaaat cgtgccagct gtatatcaag gtccgttgac
     3601 acttggggat ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac caatcgactt
     3661 atcagatatc aagaaaacgg ataaagaagg tatggcggag atcgaacgac gaacacaagc
     3721 agcttttgac aaattggaca aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa
     3781 ataaaatgat aagtttatgc gtgggcggtc ttttgaatct actgtcctgt tcagttcaga
     3841 ctttttcaat gaaaacgagg gagagacgtc aagtctgccc tctttttgcg ttccaatatg
     3901 taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt ataataatta gaaggacatt
     3961 ttttgaggtg attgaatgcg ttttttacat acagccgatt ggcatatcgg aaaaaaacta
     4021 caaggttatg acttgattga agaccagcgt catgtactat cttctatact agatatcgcg
     4081 aaagaagaaa aagtagaggc aatcgtgatt gccggagact tatatgatag gagtgtccca
     4141 gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg attggaattt aaaggaaaag
     4201 atcccgattt ttgctatatc aggaaatcac gatagcagta cgagactttc agcgggtacg
     4261 ccatggttca gttattcgaa ctattttttg cgaacacagc tagcagaagc atttgaacca
     4321 gtcgagttta aaaacgtaca atttttcttt cttccttact ttgaaccaat tgcagcgaga
     4381 ttatattttg aagatgacaa tatccgaacg atcaagcaag cggtagccaa ggtgatagat
     4441 aaaatgcagg agctatttga ttcagaaaaa aaacaagtat tgattagcca tttttttgta
     4501 atgggaagtg taaaatctga ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc
     4561 cctggtgaac tacttacacc gtttgattat gtagcactag gacatttgca taatcaagct
     4621 gctttgagac aggataatgc tcgctatagc ggttccccat tgaaattctc tttatctgag
     4681 atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa caggcctgga gttgaatttt
     4741 agggagatcg aacccctgca tgatattgaa gaaataacag cgagtttcaa agaattgctg
     4801 gatcctgcct tttatcagtc cgtcaatcgg gagaattaca tctatatcta tctcactgat
     4861 agaacgatca tcccgaatat gatgaaccag cttcggcaaa tatatcctcg tattttgggg
     4921 attgaacggg caaacggtcg agcagaacaa acaaagcgca aacgaaaagt actagaaatg
     4981 aaagatcctc aaaaattagc agaagcattt tttagtgaaa tgacggaaga gcagctaact
     5041 gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca
     5101 agaatgaaac cgaaaaaatt acaacttaaa aattttggtc cttttatcaa tgagaccgta
     5161 gatttttcac gtttgacaga agcaccgctt tttttgatca gcgggaagac aggagctggc
     5221 aaaacgacga tttttgatgg gatcaccttc gcactttttg gtgaaacttc cggaagactt
     5281 cgctctggaa aagagatgcg ttctttgttt gctacaccag acgaagaaac aagcgtgact
     5341 ttttcttttg aacatcagca gatgagctat gaaatcgaac gaaaaccaga acaagtttta
     5401 gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt gaccattttt
     5461 gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga tcagttgatc
     5521 agagaactga tgaatctcga tgcgaaacag ttttctcaaa tcgttttact accccaagga
     5581 gaattccgta attttttggt ttcatcaagc agtgataaag agactgtttt acgtcattta
     5641 ttcggtactc aatttttcca acagttcaat gagcgattaa aagaaaaagc aaaacaacag
     5701 caacaaaaat tggatcattt agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta
     5761 gtcaaagaag aggaaccagc agcagggttt gaagccgttt tagcccaatg gacaaaccat
     5821 caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga acttcaggaa
     5881 aaacaaaagc aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa
     5941 gaaaaaacga tattaataaa caagcaaaag gaattagctg ctcaaaaaga cgaaatagag
     6001 gaaaagaaac gatggattgg gtattatgaa tttacgcaac gattagtcga accaatccag
     6061 cgcctacaag aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca
     6121 aagaaactag cgatgactac gcagaattat gaagagtggc aaaaaaatga agcaaaccgc
     6181 gtacgccgac aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa acagatgatg
     6241 gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt aacaatttca
     6301 ttggataaag aagaattgaa tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa
     6361 agaactcgaa ttcttcaaga aaatttactg gatcaggaaa aactgcaaga agatcgtctt
     6421 gcttttggac agttaaaata tctagatgaa aagcagcaag aaatagaaaa acaaaaagca
     6481 aaagacactg ggtcgctcgc acagataaga gaagaactca aaaaagcaac tgaagaaata
     6541 acaaaaaata gtcagctatt gaaagaacag acagaacaag cagaacagct gaaaagccaa
     6601 tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc gtccagggga gccatgtctt
     6661 gtttgtggat caacggaaca ccccatacaa caattaaaac acgaacagcc aaataaggaa
     6721 gatatcttaa aagtagaaca acaactggca gaagctgagt ctgctgtaga aagaactcag
     6781 caaatcattg ccaaaagtga agcacatgaa aataatcttc aacaacaatt atcagattta
     6841 gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac aagaagaaca cttgcaacag
     6901 gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa caaaaacaac tcttgtagta
     6961 atcgaacaat ctttagatag acgaaaaagc aagatggcca atgcaagaat agagctggaa
     7021 gaaataagac aacaagcaga aaagctcgaa caacaattag ctgtctacaa acagcagcag
     7081 acagaaaaaa ataatcagct ccagcaactg caaggagaaa tcaaaagttt agaacaacaa
     7141 ttgcaagata cggatagtaa ctgtttagaa gaggagatac gacgacttga aaaacagtta
     7201 gaagaagact cgaatcaact agctgttgag cagaaagcag gagtgcaatt gttgcaagat
     7261 cttcatacgc ttcggacaca gcttaatctt caacatgaac aaatagaaaa aatagatgaa
     7321 aaaatagaaa aggaacaaga aagaatagct aaaaagttag aaagccagtc gtatttttca
     7381 acaattgaag aaatctgtgc atttgcacaa cagcaagcac aatataaaga aaaccaacgt
     7441 acagttactt cttatcatga agagcaatta gtgacagctg accggctgcg tcaattagaa
     7501 aatatccagc taactgagac attcccagat acagaagaga tcagccaaca gttagaagaa
     7561 ataagacgta tgatcgaaat aaagcaagaa aagatatacg gtttacaaga acaaaaaatt
     7621 agtaatcaaa aaatctacga tgaatgtctt tcgatttatc acacaagcca gacgaaatta
     7681 gatgagctta cgcaattaca gcagctttcg caaacattca acggagaaaa tcctaagaag
     7741 actagtttgg aacggtatgt gcttcaggtt tatcttcaag aagtgttaca agtagcaaat
     7801 gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg agctagcgga taccattggc
     7861 agttatcgag ggaaaaccgg attagaaatc aatatctatg atgatcaggc aggtatgtct
     7921 cgaagtgcgc ataccctgtc tggcggtgag agttttattg cagcactttc tttagcatta
     7981 gctcttgccg atgtgatcca gacacaagct ggtggtgtag caatcgatgc attgtttatt
     8041 gatgaaggat ttgggtcatt agatgaagaa gctttggaaa tggcaatgga agctttagaa
     8101 acgatcgaaa gtgaaggaag aatgatcggc atcattagtc atgtaagaga attgaaagaa
     8161 cgagtattac aacaaataag aattgataca gaaggtagcg gacaaagcaa aatacgctat
     8221 caacttggat aaaaagggag attggtaact atgaaatgga gtattccaga aaaagtcatt
     8281 gcacgtggac gtcaatactt gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa
     8341 gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt atatggttga tttggatggt
     8401 acagcaaaag agatagatta ctgccaatgt ccctattggg aggaacataa atattgtaag
     8461 catacagttg ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa
     8521 gaccagccaa tgctagaacc ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt
     8581 ttttctcgtc taaaaacaca aaacaatcct caagcggtgc aacctttgat tattgaatac
     8641 caagtagaaa cgattgagac taatcagtat cacccagagc tttctatttt ggcactttac
     8701 ttgcgtatcg gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa catctatgat
     8761 tttttacaag cttacacaga aaaagaaact tacactgtta ataaacagta ccagtttcgt
     8821 ctagataaac gggcgtttca aaaagaagat caaaaaatac tcacgcatct tgcaggaagt
     8881 gctcagacgc atcaattatt aggtacgaac ggcttacaag tcaaaggaaa actagataaa
     8941 agatatctgc ttctcccagt agaacaagcc cgcttcctgt tagagcagat gaatcagact
     9001 acacgtttgc atctgcagat aaatgaacaa aacgatcaag gaatacaatt ttccgatcaa
     9061 aacaaacctt tatcattcaa agtggaaaaa tttggagaag attatttgct gcaagcattg
     9121 aatgattttg attttttctt tatgcattat cagtggggag ttatccaagg gacattttat
     9181 tctctgacga aatatcaaca aacggtttat caaacttgga aacaattgat tcgccgattg
     9241 gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa agagattatc
     9301 cctctgctgt cagagatcgg cgacgtagct attgcccctg cagtagaaga ggaagtagca
     9361 gatatccccg tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga
     9421 gtcgatttcg tctatggtga agtcatctat tcgactgacc cgaagcatga ggtctcgaca
     9481 gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga aacgagtact tgatctattc
     9541 agaatgtatc gctaccagaa aaatgaaact ggttatgaac gtccattgcc aagcggggaa
     9601 gaactatatg cctttttccg cacagaacta tctgtcttcc atcagtttgg tgaagtcaga
     9661 cttggaagaa aattgcgaga gttgtattta gatgcgcata aacatcaacc gaaaatcgaa
     9721 gtaaccgaga ccggttcatg gctcgatatt cgattcgatg tgacagggat agaggagcaa
     9781 gaaatcgatc atgtcctgca aagcttgctg agaaatgatg ctttttatac attagaaaat
     9841 ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa
     9901 ttacgggaat cgatacgtac agaagaagga acgatccatg tacctaaaaa tcaaggattg
     9961 atcattcaaa atcaattgga aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg
    10021 gtacaagatc tcattcatcc agaaagatac caggcacagc ttcccaaagg attgaatgca
    10081 acgatgagag attatcaaaa gcaaggtttt cgatggttga agatgttagg tcattatcag
    10141 tttggcggta ttttagccga tgaaatggga cttgggaaaa cactacagac aattgccttt
    10201 ttactttcgg aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc
    10261 ttaatttaca actggcaggc agaggtgaga aaattcgccc cttctttaag tatccaagta
    10321 atcaatggga ataaaaaaga acgtgaagaa ttactggcta aagacacaga tatccgagtg
    10381 acttcttatg ccagcttaag acaggatcta gctaattatc agtcgcaaaa aattgattat
    10441 ttgattcttg atgaggcaca aatggtcaaa aatagcagta cgaaaactgc acaagcattg
    10501 agagaattag ctgtacctca gcggtttgca ctgagtggga caccaatcga gaataattta
    10561 gaagaattgt ggtcattgtt tgcaacgatt atgccaggat ttttcccgac taaaacaaaa
    10621 ttcagggaac ttgccgcaga agaaattgcg caaatgattc gaccgtttat tttacggcga
    10681 gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag agatgaatct atattctgca
    10741 cttacagaag aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc
    10801 acatcaatgg attctgaagc attcaagaaa aaccggatcg ggatacttgc tggtctgaca
    10861 agactgcgac aaatttgctg cgatcctcgt ctgtttattg aagattacca aggcggttcc
    10921 ggcaagctcg aacaagtaaa agatctactg gtagctgcca aagagaacaa acgaagagtt
    10981 ttgctgtttt ctcaatttac agggatgctg actatcttgc aagaagagtt agccgagcta
    11041 gggatctcga cattttattt aagaggcagt acgaaaccac aagatcggtt aagtatggta
    11101 gacgcattta atgctggcga aaaagatgtt tttctgattt ccttaaaggc tgggggaact
    11161 ggattgaatc tcacgggagc agatacggtc atcttgtatg acctttggtg gaatcctgcc
    11221 attgaagaac aagctgctgg acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg
    11281 tggcgtatga tctctgaagg aacgattgag gaacgcatgg attcactcca gcaagaaaaa
    11341 cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac agttgacgaa actgactgaa
    11401 gaagacatcc gcatgatttt aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc
    11461 gttcagtaat gggtaagaaa gcctttctaa agaagcgatt gtttatttga tttaaaaaat
    11521 agcaaggagg ctgggattcc attttatccc agcttccttg ctatcatttt tttatttcta
    11581 tttttaaagg ttatacaaag attgtcttca ttgtctctaa cagctgatca aaagaagaaa
    11641 tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg
    11701 cttgccagtt agcacttttt gcacctagaa catcattgtc gtaattatcg ccaacgtata
    11761 aggtctcttc tggaagcata tgaaattctt tctcagccag ctgaaaaatc tctttttctg
    11821 gtttttggaa acctgttgct tgagaaatga gcatattgcg ggaaggaatc caattgttca
    11881 attgaagttg cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg ataattccta
    11941 atgggacatt cttttccttc aagaaatcga gggttttttt gacctcctca tgcaagcaga
    12001 tattatcaag ttcttcctcg taaatttttt gaaataagag gccatcttct tccgtaatat
    12061 gagggtagtc taaatctttt agtgattggc tgatccgatg tgctcgcata tattctagtg
    12121 tccaatctcc tgccattact tttgggaagt tttcatcact atgatggcgg aaccggatat
    12181 atagtggatg catatctgca tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa
    12241 atggctgctg ctgatcatac atcgtatcat caacatcaaa cacaactgat ttaatcaaaa
    12301 ttttacacgc ctttcggttt tcattgattt tttcacaaat ccttgactat tgtaacagaa
    12361 aaaattgtct aaaaaagctg ataaataaag gtttttctta ctatatttct atttccttga
    12421 cagaataagg tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag
    12481 tttgatgaac aaattaatca ttatggtaga agtcgattga
//
LOCUS       DAZUKD010000079        12190 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000079 DAZUKD010000000
VERSION     DAZUKD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.081"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..316)
                     /locus_tag="ACG8N9_001928"
     CDS             complement(152..316)
                     /locus_tag="ACG8N9_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897493.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            611..1939
                     /locus_tag="ACG8N9_001929"
     CDS             611..1939
                     /locus_tag="ACG8N9_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4897494.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2141..2974
                     /locus_tag="ACG8N9_001930"
     CDS             2141..2974
                     /locus_tag="ACG8N9_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4897495.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2990..3727
                     /locus_tag="ACG8N9_001931"
     CDS             2990..3727
                     /locus_tag="ACG8N9_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4897496.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3882..4850)
                     /locus_tag="ACG8N9_001932"
     CDS             complement(3882..4850)
                     /locus_tag="ACG8N9_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4897497.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5082..5915
                     /locus_tag="ACG8N9_001933"
     CDS             5082..5915
                     /locus_tag="ACG8N9_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4897498.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5949..6788
                     /locus_tag="ACG8N9_001934"
     CDS             5949..6788
                     /locus_tag="ACG8N9_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4897499.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6814..7374
                     /locus_tag="ACG8N9_001935"
     CDS             6814..7374
                     /locus_tag="ACG8N9_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4897500.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7573..9294
                     /locus_tag="ACG8N9_001936"
     CDS             7573..9294
                     /locus_tag="ACG8N9_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4897501.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9459..10601
                     /locus_tag="ACG8N9_001937"
     CDS             9459..10601
                     /locus_tag="ACG8N9_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4897502.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10617..11828
                     /gene="thiI"
                     /locus_tag="ACG8N9_001938"
     CDS             10617..11828
                     /gene="thiI"
                     /locus_tag="ACG8N9_001938"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4897503.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2047 c         2532 g         3459 t
ORIGIN      
        1 tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt
       61 ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc
      121 tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata
      181 cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga
      241 cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag
      301 caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa
      361 tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac
      421 aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt
      481 atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata
      541 gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa
      601 ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca
      661 agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc
      721 tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac
      781 ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac
      841 attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta
      901 cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac
      961 aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga
     1021 tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc
     1081 ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa
     1141 attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga
     1201 cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc
     1261 attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca
     1321 tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga
     1381 tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac
     1441 agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat
     1501 ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg
     1561 ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc
     1621 aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg
     1681 agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc
     1741 aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt
     1801 tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt
     1861 gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc
     1921 aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga
     1981 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
     2041 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
     2101 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
     2161 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
     2221 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
     2281 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
     2341 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
     2401 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
     2461 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
     2521 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
     2581 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
     2641 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
     2701 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
     2761 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
     2821 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
     2881 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
     2941 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     3001 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     3061 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     3121 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     3181 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     3241 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     3301 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     3361 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     3421 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     3481 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     3541 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     3601 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     3661 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     3721 taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg
     3781 aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt
     3841 cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa
     3901 atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt
     3961 tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct
     4021 tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg
     4081 attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca
     4141 cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc
     4201 ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc
     4261 agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg
     4321 atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg
     4381 tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct
     4441 tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca
     4501 ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca
     4561 agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata
     4621 cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg
     4681 tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg
     4741 atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt
     4801 tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact
     4861 tccttacgaa taattttgca tagccgttaa atcttattgt atactatata aaagagtttt
     4921 gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa
     4981 tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac
     5041 aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa
     5101 catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg
     5161 cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct
     5221 cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc
     5281 aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg
     5341 cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag
     5401 tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta
     5461 gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg
     5521 atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca
     5581 tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga
     5641 actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag
     5701 agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac
     5761 atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt
     5821 ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag
     5881 atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg
     5941 cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt
     6001 caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat
     6061 gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat
     6121 tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg
     6181 accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc
     6241 gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac
     6301 aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga
     6361 tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag
     6421 agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa
     6481 agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata
     6541 tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa
     6601 ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag
     6661 gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact
     6721 ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga
     6781 tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa
     6841 gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca
     6901 gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca
     6961 gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcattg
     7021 gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa
     7081 caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc attactagga
     7141 atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca
     7201 gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag
     7261 atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg
     7321 attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag
     7381 tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt
     7441 gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat
     7501 tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg
     7561 gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc
     7621 gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat
     7681 gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat
     7741 gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa
     7801 tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa
     7861 aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct
     7921 acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg
     7981 gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg
     8041 aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa
     8101 cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac
     8161 cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct
     8221 ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt
     8281 gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa
     8341 gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt
     8401 gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat
     8461 tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa
     8521 tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt attgattgag
     8581 ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt
     8641 ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt
     8701 caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta
     8761 gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc
     8821 gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt
     8881 tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt
     8941 gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa
     9001 gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca
     9061 catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat
     9121 cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa
     9181 gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct
     9241 ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa
     9301 aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta
     9361 tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg
     9421 ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg
     9481 caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta
     9541 ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag
     9601 caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag
     9661 gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg
     9721 ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat
     9781 taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg
     9841 tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga
     9901 ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc
     9961 ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat
    10021 ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg
    10081 gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag
    10141 ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta
    10201 aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa
    10261 aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa
    10321 ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg
    10381 tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta
    10441 aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg
    10501 ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta
    10561 ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga
    10621 aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa
    10681 gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca
    10741 aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag
    10801 ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag
    10861 aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg
    10921 ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta
    10981 atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc
    11041 aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat
    11101 cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc
    11161 ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag
    11221 tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa
    11281 aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag
    11341 tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga
    11401 caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt
    11461 tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg
    11521 tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac
    11581 ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg
    11641 aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg
    11701 tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct
    11761 tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat
    11821 tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa
    11881 aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc
    11941 atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta
    12001 tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc
    12061 gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt
    12121 tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta
    12181 tcagaggttg
//
LOCUS       DAZUKD010000080        12136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000080 DAZUKD010000000
VERSION     DAZUKD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.082"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACG8N9_001939"
     CDS             <1..610
                     /locus_tag="ACG8N9_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4897504.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACG8N9_001940"
     CDS             723..1001
                     /locus_tag="ACG8N9_001940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4897505.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACG8N9_001941"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACG8N9_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4897506.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACG8N9_001942"
     CDS             1458..2213
                     /locus_tag="ACG8N9_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4897507.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACG8N9_001943"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACG8N9_001943"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4897508.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACG8N9_001944"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACG8N9_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4897509.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACG8N9_001945"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACG8N9_001945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4897510.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACG8N9_001946"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACG8N9_001946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4897511.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACG8N9_001947"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACG8N9_001947"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4897512.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACG8N9_001948"
     CDS             6950..7492
                     /locus_tag="ACG8N9_001948"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4897513.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACG8N9_001949"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACG8N9_001949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4897514.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACG8N9_001950"
     CDS             9085..9987
                     /locus_tag="ACG8N9_001950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4897515.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACG8N9_001951"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACG8N9_001951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4897516.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACG8N9_001952"
     CDS             11430..11852
                     /locus_tag="ACG8N9_001952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4897517.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAZUKD010000081        12036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000081 DAZUKD010000000
VERSION     DAZUKD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.083"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..324)
                     /locus_tag="ACG8N9_001953"
     CDS             complement(<1..324)
                     /locus_tag="ACG8N9_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4897518.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIY"
     gene            complement(330..1193)
                     /locus_tag="ACG8N9_001954"
     CDS             complement(330..1193)
                     /locus_tag="ACG8N9_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897519.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1207..2103)
                     /locus_tag="ACG8N9_001955"
     CDS             complement(1207..2103)
                     /locus_tag="ACG8N9_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897520.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2278..3567)
                     /locus_tag="ACG8N9_001956"
     CDS             complement(2278..3567)
                     /locus_tag="ACG8N9_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4897521.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3539..5056)
                     /locus_tag="ACG8N9_001957"
     CDS             complement(3539..5056)
                     /locus_tag="ACG8N9_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4897522.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5254..6144
                     /locus_tag="ACG8N9_001958"
     CDS             5254..6144
                     /locus_tag="ACG8N9_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4897523.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6217..7041)
                     /gene="nadE"
                     /locus_tag="ACG8N9_001959"
     CDS             complement(6217..7041)
                     /gene="nadE"
                     /locus_tag="ACG8N9_001959"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4897524.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(7053..8525)
                     /locus_tag="ACG8N9_001960"
     CDS             complement(7053..8525)
                     /locus_tag="ACG8N9_001960"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4897525.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(9162..9524)
                     /locus_tag="ACG8N9_001961"
     CDS             complement(9162..9524)
                     /locus_tag="ACG8N9_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4897526.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            9744..10496
                     /locus_tag="ACG8N9_001962"
     CDS             9744..10496
                     /locus_tag="ACG8N9_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4897527.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(10636..11559)
                     /locus_tag="ACG8N9_001963"
     CDS             complement(10636..11559)
                     /locus_tag="ACG8N9_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4897528.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(11574..11846)
                     /locus_tag="ACG8N9_001964"
     CDS             complement(11574..11846)
                     /locus_tag="ACG8N9_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897529.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         3782 a         2426 c         1966 g         3862 t
ORIGIN      
        1 ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa
       61 ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa
      121 atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca
      181 ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca
      241 cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata
      301 aaatttagtc tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct
      361 tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc
      421 gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct
      481 gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc
      541 caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac
      601 atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa
      661 gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata
      721 cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat
      781 agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa
      841 actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat
      901 acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa
      961 gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta
     1021 ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa
     1081 ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca
     1141 ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt
     1201 tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat
     1261 agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg
     1321 tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc
     1381 accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa
     1441 aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg
     1501 atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc
     1561 agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt
     1621 cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa
     1681 caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga
     1741 cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt
     1801 atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag
     1861 tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg
     1921 tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa
     1981 actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga
     2041 tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt
     2101 catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct
     2161 gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag
     2221 gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta
     2281 tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc
     2341 tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg
     2401 ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat
     2461 aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt
     2521 ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac
     2581 agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat
     2641 agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc
     2701 gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc
     2761 attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg
     2821 gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt
     2881 agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc
     2941 agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg
     3001 tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg
     3061 tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata
     3121 ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac
     3181 tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc
     3241 aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg
     3301 aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc
     3361 atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa
     3421 cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc
     3481 accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt
     3541 acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata
     3601 gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg
     3661 gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag
     3721 aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt
     3781 cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt
     3841 ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc
     3901 ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc
     3961 tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacgtttacc aattgagcaa
     4021 gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata
     4081 gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg
     4141 ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt
     4201 tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag
     4261 aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt
     4321 tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg
     4381 ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct
     4441 caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt
     4501 cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg
     4561 tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc
     4621 ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca
     4681 gattgacagc catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa
     4741 tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg
     4801 ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc
     4861 taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt
     4921 tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt
     4981 caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca
     5041 acattgtggc ttccataact gacctccttt aatttataca tgaataatac acctcgttga
     5101 aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt taattaacca
     5161 aaatattaat aatgattaaa acaaataatt taatataact tttattaaca aatatgttaa
     5221 tatataagaa aaacctttta agaggtgata tatatgcaac tacaaataaa cgaagaatcc
     5281 ttaccggtat atgaagcatt agctagtaaa acaaggatca agatcatcca gctactttcc
     5341 aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg gagtgagcag tgctatcacg
     5401 acgatgcacg tgaaaaagct agaggaagca aacatcatca aaacagaaaa agtaggacaa
     5461 caaaaaattt cgagccttcg tgtcgataaa atcgatatta gttttccaga aaaaatattc
     5521 aatgcttttg atacgaaaga aacatcgata cctattggtc actatacaaa ttatgcaatt
     5581 gaaccaacat gcggtttagc tacgatccat gattttatcg ggaaagtgga tgaaccacgc
     5641 tattttatgg atcctcgaag gatggatgcc cgcattctct ggtttaccag cggatttgtc
     5701 gagtatcagg cacctaattt tttaaataca gaggatacat tggagatgtt ggaagtttct
     5761 gtggaaattt cttctgaatt tcctttttca aatgacaatt ggccttcaga tatcactttc
     5821 agcctaaatg gtgtggagct aggaacatgg acgagccctg gagattttgc tgatatccgc
     5881 ggtaaatata cgcctgattg gtatccagat aatcttaatc aatacggctt gctgaagacg
     5941 atccgaatta ccaaacactt gacgaatatg aatggagaac ccttgtcaaa catcacgatc
     6001 aacgacatcc ctaaagagca agacacttgg catttgcgaa tcgaagtgaa agacgatgcc
     6061 aaacatgtgg ggggctgtac tttatttggt aaaggattcg gtaattacga tcaggatatc
     6121 aaattaaaag tttactacag ctgatatttt tcttttctat tttataaaac aaaaacagcc
     6181 aatccctatg aagggacggc tgtttttgct ttattgttat ttccagaaat catccaaaac
     6241 ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac catgcttcga ttttttcttg
     6301 tgcttgagcg ggaacttctt ttccttctag ataatcatca atttcattat atgtgacacc
     6361 tagtgcgact tcatctgcaa tcattggttt cccatcttca agatctgcag tcggaatttt
     6421 tgtgtataat ttttccggtg cattcaatgc ttgaagcaat tgtttccctt gtcgtttgtc
     6481 caaacggaat aatggcaaaa tgtcggctcc accatcacca aatttcgtga agaatccagt
     6541 aatgttttct gcagcatgat ctgtccctaa tacggctcct gctctttctc cagctacggc
     6601 atattgcgta atcatacgtt gacgtgcttt gatatttcct ttgttaaaat cgctgattcc
     6661 cacacctgct tcgcttaata cttgtacttg tgcatcaaca gctggtttga tatttactcg
     6721 cagactaaca tcgggctgga taaagtcaag agcagctttt gcatcttctt catctgcttg
     6781 ttcaccataa ggaagacgaa cagcgataaa ttgatagttt tcatcttttg tttcttcacg
     6841 catttcttcc atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt ggccgccgct
     6901 gatacctagt acaaaagttt taagaaacgg atattttttc aaatatgctt tcataaagtc
     6961 aatactttta cgtatttctt cttttggatc aattgttggt ttgacaccta attcattgat
     7021 aatttctgtc tgcaggctca tttattctcc tcctatgctt ctgtatgaag ttctgctacg
     7081 ctttttttca ttcgttccat gatagccatt ttatggttcc agcattcagt agacagatcg
     7141 actgggtatt tttgcggatt caagtctcgt ttatactctt cccatagtga atctagattt
     7201 tcttttgtat attctttgat ttcgtttagt gcaggtaatt cgtaaactct ttttccttcg
     7261 ataaagatat cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt attgataaac
     7321 gtatgcactg gatggaacat gaagatagct tcttcttctc gagggtcttc atcccataat
     7381 gtcacgtagt ctccttcaga tttcccatca aaatttcgag tgatgcgcca cacttgtttt
     7441 ttccctggag tagttacttt ttctgcattg cttgataact tgatcgtatc catcattttt
     7501 ccttcatcat cttcgatcga tactagcttg aatactgcac caagggctgg ctgatcatag
     7561 gctgtgatca attttgtccc aacaccccat acatcaattt tagctttttg catctttaaa
     7621 ttcaaaatcg ttgcttcatc caagtcattc gaagcataga tttttgcttc cgtaaatccg
     7681 gctgcatcta attgttcccg aacacgctta gatatatagg ccatatctcc actgtcgatg
     7741 cgtacgcctt ggaagttgat tttgtcacct aattctttcg ccacgcgaat cgcacttggc
     7801 acacctgatt tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt atgggtttta
     7861 gcgtaggcca taaatgcttc ataatcattt ccataagatt ggactaacga atgagcatgg
     7921 gtcccgctag ctggtatccc aaatatcttc ccagcacgta cattgcttgt tgcatctgct
     7981 ccgccgatat atgccgctct agttccccag actgctgcat ccagttcttg tgcccgtcta
     8041 gttccaaatt ccaatagtgg atcttctccg atgacagatt tgatacgagc tgcttttgtc
     8101 gcgatcaacg tttggaaatt gaccatattc aacagtgctg tttctactaa ttggcagtgc
     8161 gctaaaggcc cttccacttg gatcaatggt tcatttgcaa aaactagttc tccttcacga
     8221 gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa accttcagga
     8281 tagacttcta atccacgcaa gtaagcaata tcggaatcat tgaacgtcag attctctaaa
     8341 taattgacta accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa aggcatttct
     8401 cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac ctaattccca gtatgtctgc
     8461 atcatattga tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg gtaagtatta
     8521 ttcatcttgt attactcctc ttggcaagtt ttcatttcct acccattata acaaaaaaaa
     8581 ttaataggaa aagaaatctt ccattttttc tgaaaaagca catgttttac ggatatttct
     8641 tgctaaaaat gaactccatc tgctaaaact atcctttctt attggtattt aacctaaacg
     8701 agaaagaata cttattttat tcaatgtatc cgagacacga agaaattagc gttttctatt
     8761 tttcttactt gataaaataa gtagattttc ctactacaat cgtagccgtt aagataccga
     8821 tgattgaaca aatataacca aatgtctcta caacataatc aatggcaaaa gtttttgaag
     8881 caataatacg tctttccttt tttgcttctt atccccaaag aaacggatat aacttattat
     8941 gtattgaatt tttttggaaa ggcgaattat gacaagatat accttgcgtt gaaaaaactc
     9001 gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta agaaagcagc tgtcaataga
     9061 atggaaacaa aaatggcgag acatcaatca gtctcgcctt gttttttatt gtatacatta
     9121 gatatggagc aggttaccat aaagatgatt attagtcgtt attaaataga atccttcgta
     9181 tttttttgtt ctatcactac aatatcttta tttggacgtt tgaattgtat tccggcacgt
     9241 tctagctcct gtttcactcc tacatggact ttgacatctt ctcgtgtcat ttttaaaacg
     9301 tgatgaatcg catagtcgat ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg
     9361 acttgctttt tatcatatgt gtctaccaat agcagttgtg ttcgatctaa cgaaaataat
     9421 ttgtaataat taatagccgt attcccaaaa aagaattcca ctactttagg ataaaatgtc
     9481 ttaatattta aattactatt cacaggtgtt tcgattagct tcatctcatt cctcttttct
     9541 atatagtcga tttagatcca atcttaagtt atgttacctt tcctagcatt aaggaatcgg
     9601 ttacaattga acctttaaag acaaaaggat ttacctttta tcacttaata gtataacata
     9661 atttcatgag aaaaaaatac aattttttct tgaccatctc gttacgtaaa ggattatgct
     9721 tacatttgtc aggaggaaaa ataatggagt acacaataaa gaaaatgtca gagatatcag
     9781 gagtcagtcc gagaaccttg cgcttttatg atgagattgg actgctaaaa ccagctagga
     9841 tcaattcttc aggttatcgg atttatggca aaaaagaagt cgatcgtctg caacacatct
     9901 tattttatcg aacaatggca ttcaagttgg atgatatcca agaggtctta gacaatccat
     9961 cttttgacca ccagaaagct ttaatcaagc atcagcaaat gttgctggaa aaacgggcac
    10021 aaatcgatac cctcttgaca actgtccaac agacacttga tatgtacgaa ggaggaagaa
    10081 aaatgagtga caaagaaaaa tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag
    10141 aatcctatgg acaagagatt cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg
    10201 aaaaatggat gaatatggat aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga
    10261 tgttatttga tctcactctt tacttaaaag aaccagaaga tgaaagctta gcagaccgta
    10321 tcttccaagc tcataagaag tggttatctt acagctggcc tgaatatcag ccaaaagcgc
    10381 ataaaggact aggaatgatg tacgaagccg atgaacggtt cactgcatat tatgacgagc
    10441 gaagtggtca tggtgcagca aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt
    10501 ttacatgatc caacaaaaaa gtacacgtaa aatgacctaa taacaaaaca gcagacaccc
    10561 aagttagatt tttatgctaa cttttggtgt ctgctccatt ctttcacaag ttttttcatc
    10621 ctactttaaa tatattcatc gttgaataaa cgtataaggt attttcactt tatccttttt
    10681 ttctgtaaga aacaattgaa atgccatttc tccgcaagct tccagatggt ggtcgatggt
    10741 tgggaaacca agaagctcgc tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt
    10801 atcttgcaga tagctttgca agatgcctgc tgcaaccata tccgaatttg taaagacacc
    10861 atcaactcca agctctttaa aaaagaagcc tgcttgtttt cctttctctg catctataca
    10921 atcccaaaaa acaagtgatt catcaaaaga tggaaaaagt tgatgacata gctgcaaagt
    10981 gatttttgag ttataactta gcctgccact tcgccccaac gtgatgccca attgctttat
    11041 accttgctgc ttcaaataag taagaccttc tgtaattgat tgttctcgat caataaagac
    11101 acagcttgcc tgttttccct tgatttcttc acaaaaaact agaggtccat atttttgata
    11161 tgccaataaa tcatctagtg tattggcacg agacgtaatg atcaatccat cgaaagcttt
    11221 agctgcaaat tcttcaagat accttcgctc tttttctttc tgatagcccg tcggtaaaag
    11281 tgtcactttg taatcataat caaacgctgc ttcaatgatc ccactgatca actgatcaaa
    11341 atacggatga ttggtataag gcagaatcac tccaatgctt ttcgtttttc cataacttaa
    11401 ctgtctggca tttgcattag ggatataatc gacttctttc atgaccgcaa gtatttcttt
    11461 ccttttttcc tcatctacat atggatgtcc attgattaca cgtgaaatag tagaaactga
    11521 ataacctgtg agctttgcga tatctcgaat gtttgccact gctgcttcgc cacctataca
    11581 aaaagatttt cttgtaaata gtctaccaca gctggaccgc attcaaaaaa ctgatacatt
    11641 tttccttcat gcgaaaaact gatccgctgc ccgccccaat atatatcttt tgtcaaattt
    11701 tcgatttcat tcattggtat ttcttttact atattcctat tcaacccttg aaaactgtac
    11761 aacgttaata cggatccctt gaactcgaca aacgcataaa aataattgtt cgttgtgcca
    11821 tctgttatct ctacataagt attcattgca agccctcctt ttgatacttt gagtatagtg
    11881 tatgaaacgc gtctcatagc aaggagtttg ttttgccaaa gaaaagtttt attcctcttt
    11941 cattattacc aaagtctctt attttaaatt gaatacatta taatgatact gtaaatatat
    12001 ctttattatt agtgaatgga tatttacttt taatag
//
LOCUS       DAZUKD010000082        12002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000082 DAZUKD010000000
VERSION     DAZUKD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.084"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..702)
                     /locus_tag="ACG8N9_001965"
     CDS             complement(115..702)
                     /locus_tag="ACG8N9_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897530.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            877..2199
                     /locus_tag="ACG8N9_001966"
     CDS             877..2199
                     /locus_tag="ACG8N9_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4897531.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2354..3130
                     /locus_tag="ACG8N9_001967"
     CDS             2354..3130
                     /locus_tag="ACG8N9_001967"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4897532.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3123..3692
                     /gene="rnmV"
                     /locus_tag="ACG8N9_001968"
     CDS             3123..3692
                     /gene="rnmV"
                     /locus_tag="ACG8N9_001968"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4897533.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3685..4569
                     /gene="rsmA"
                     /locus_tag="ACG8N9_001969"
     CDS             3685..4569
                     /gene="rsmA"
                     /locus_tag="ACG8N9_001969"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4897534.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5020..5649
                     /locus_tag="ACG8N9_001970"
     CDS             5020..5649
                     /locus_tag="ACG8N9_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897535.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5773..6882)
                     /locus_tag="ACG8N9_001971"
     CDS             complement(5773..6882)
                     /locus_tag="ACG8N9_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897536.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7091..7513
                     /gene="mgsA"
                     /locus_tag="ACG8N9_001972"
     CDS             7091..7513
                     /gene="mgsA"
                     /locus_tag="ACG8N9_001972"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4897537.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      7700..7798
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            7836..8372
                     /locus_tag="ACG8N9_001973"
     CDS             7836..8372
                     /locus_tag="ACG8N9_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4897538.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8412..10175)
                     /locus_tag="ACG8N9_001974"
     CDS             complement(8412..10175)
                     /locus_tag="ACG8N9_001974"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897539.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10178..11893)
                     /locus_tag="ACG8N9_001975"
     CDS             complement(10178..11893)
                     /locus_tag="ACG8N9_001975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897540.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3739 a         2221 c         2451 g         3591 t
ORIGIN      
        1 tcactttatg ttcaattagt ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa
       61 acaatcatcg gctttgactt cgatgattgt tttctttatt ttgtaaaaat cctatcactt
      121 gattttatgg taacctttta tttttgtctt caaacggctt ggtaagagag cagtaagcag
      181 tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt ccgataccat aatgttgata
      241 gagctttgtc aacatcacgt gtaacaagat caaccctaaa agcaaggagc cagctataaa
      301 ccaatgatta tcccaatgca tgacttcact aagtgccatc actccaaaag gatagaaata
      361 aaaatagatc ccgactaagg tcatccgcca gttctttgta aatgttccga tattcaataa
      421 aaagaaaatc gctgaaagca aaataccgat aggcggaaaa agcccaacca aaaaggaata
      481 caacatcccc cacaagatca tcacaggacc tttgatcaat gcaacgattg cgatgaacaa
      541 agtcagcaat attagttttt gtgataacgt tgcaacagtc attaatccaa ataaaattaa
      601 tgtgaaccaa tgccaatggg actgctgttg ttctttacgg gaaaaatcga cccggttgga
      661 taaataatct gctaatttcg gataagaacg cttaaaatcc ataaactgtt cctccttcgt
      721 ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat acatccgtct taagagggag
      781 tttttcactg aaagtggaga aaaaagaatg gtaaattaaa atattctcag ttatactgaa
      841 aattatacca aatttcaaag aagaaagagg atgggaatga aaggcaaact aaaacgagaa
      901 atcaatctgt ttggcgcatt agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc
      961 ttcaaaactg ctgctgttac tgctagtact cagtctacaa gtttgacatt gctagcttgg
     1021 cttttgggtg gtttcttaac gatctgtgct ggattgacag tggctgaatt agctacagca
     1081 atcccggaaa ctggtggagc tgtaaaatat attgaagctg cgtatggaaa attgcccagc
     1141 tttttattag gatgggcgca aagcttgatt tactttcctg ctaatattgc tgcattatcg
     1201 attatctttg cgacacaatt gacgaactta ttacaattat ctaccgatta tcttttgttg
     1261 attgcagtga tcaccgctgt ttctgtgaca gggttgaatt tgttagggac aaaagtcggt
     1321 gcttctgtac aatcagtgac gttgattgtt aaattaatac caattgctgt gattgtcata
     1381 tggggattat tgaccccagg acaaggaact gtccagttat ttccaatcga agctggaaaa
     1441 gatgttactt ttgtcgaagg attgagcagt gcgttgttag caacgttgtt tgcttatgac
     1501 ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac ggccggaaaa agatttgcct
     1561 aaagcaatta ttcttggttt gagttttgta acagtcgttt atctattgat caattttgtt
     1621 tttttgaaaa ctttgccgat tgatcatctt gccggtaatt tgaatgcagc ttctgaagca
     1681 tcagacgtca tttttggagg aatcggtgga aagctggtca ctattgggat cttgatctcg
     1741 gtatacggtg cattgaacgg ttatacacta actgggatcc gcgtgcctta tgcgatggct
     1801 ttagaagacg agctaccttt cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt
     1861 ccttatgttc ctgcggtttt ccagttagca gtagcttgta ttatgatgag ccttggttct
     1921 ttcgactttt taacagacat gttgattttt gtgatgtggc tattcagttt actgattttt
     1981 atcggtgtct ttattttaag aaaaaaagcg ccggaattgc cacgtccata taaagtacca
     2041 ctttatccaa tcgttccgat tattgcgatt ctcggtgcta ttttcatttt aggtatgacg
     2101 atgatgacgc aaacgaaatt agcattgatc ggtataggtg ttaccttgat aggtatccca
     2161 gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa atatttcaac aacagacaga
     2221 aaaagtacga actaggtcct ctagatcttc gtgctttttc tatttcaata aaacaagttc
     2281 ttctattttg atttttgcta cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa
     2341 aggagagata aacatgatat ttgattctca tacccattta aatgctgaac agttcaatga
     2401 agatattcca gaaacgattc agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt
     2461 gggcttcgat acgccaacga ttgaaaagtc tttgcaatta agccatgact atccaaatat
     2521 atacagtatt atcggctggc atccgacaga ggctggcagt tatacgaaag aaatcgaaaa
     2581 taaattacaa gaacaattga ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga
     2641 ttatcattgg atggaagacc cgaaagaagt ccaagataaa gtatttcgca gacagattgc
     2701 tatcgcaaaa gaaatgaacc taccaatcag tattcatact agagaagcac ttgaagacac
     2761 gtatcaaatc ctaaaagaag aagatgttcg agatatcggt ggaatcatgc atagtttcag
     2821 tggtgattat gaatgggcaa aacgatttct tgacttaggg atgcatattt catttagcgg
     2881 tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct gcagcaaatg taccaatcga
     2941 acgattactt gtggaaacag atgcccctta tctggcacca gttcctttta gaggaaagcg
     3001 aaatgaacca ggctatactc gttatacagt agaaaagatt gctgaacttc ggaatcttcc
     3061 gacagaagaa gtggctgccc aaacatggac aaatgcgcac cgattatttg gtctaagcga
     3121 acatggataa gatgaagatc caagagatta tagtcgttga aggaaaagac gatacaagaa
     3181 ggctgcggga agtagttgat gcagatacga tagaaacaat agggtctgca atcaatgaag
     3241 agatcctgat gcaaattgaa catgcccaag agactcgtgg agtgatcgtt tttaccgatc
     3301 ctgatttttc aggagaaaaa ataagaaaaa tcattatgga agctgttcct caggcaaaac
     3361 atgctttttt gccgcgcaca caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc
     3421 atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc
     3481 aaacggctgt ttctgaaatc actagacaag accttttaga ctatggttta atagctgggg
     3541 cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg
     3601 gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag
     3661 caatgaaaat agtggaggaa caacatgagt gaatatcgtg aaattgcgac accctctaga
     3721 acaaaagaga ttttattgaa acatggtttt tcatttaaaa aaagtttagg acaaaatttt
     3781 ctgactgaac caaatatttt aaggaaaatc gtagaaactg cgcaaataga tgaacatacg
     3841 aacgtgatcg aagttggacc aggaatcggt gcattgacgg aacaattagc caagcatgca
     3901 aaacaagtcg tagcatttga gatcgatgat cgtttgatcc ctgtattggc tgatacgatg
     3961 caaccctatg acaatgtgac tattgtacat caagatattt taaagaccga tttgtcaaca
     4021 gctgttagag aaacatttca tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac
     4081 tatattacga caccgatcat gatgcatttt cttgaatcag atttggttgt ggatgaacta
     4141 gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg ctgaaccagg aacgaaagct
     4201 tatggttcat tatcgattgc tgttcaatat tatatggaag ccagtttagc attcatcgta
     4261 ccaaaaacag tatttgtccc tcagccaaat gtagattcag cgattttgaa actgacaaga
     4321 agagatatac cggcagtcga agtgacagac gaaaaagcat ttttccgatt gacaaaagcg
     4381 gcatttcagc aaagaagaaa aacgctttgg aataacttgc agcattcgta tggcaaagat
     4441 gaccagacaa aagcatggtt agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt
     4501 cgtggggaga ctttgtcatt acaagaattc gccgcattaa gcaatgcgat gtcagaaaat
     4561 atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat
     4621 aagaacagcc aaacaaaaat agttcctcat gtttttcgtt tgtttgggct tattaccgca
     4681 gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg gcctgccgtt taactctaag
     4741 tcacaaggaa caatttcaaa aaacagcttc tcatgttttt gagttagttg gtcttattac
     4801 ttgatgcaag cttttgacca ctattatgcc gaacaaggag atgtgacaca aattttgtca
     4861 catctcctat ttttgtttct tgatcaaaaa attcttatac ttgatactca cactcatttt
     4921 gtatggattt agaaaagaat atgtgtcttg acagttatta gtaagtgagt tatactaagt
     4981 cgacatttat gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa ttcagttcga
     5041 aaactaacga tttctgcttt attgattggg atgggggtca ttatcccaat ggtcatgcct
     5101 aaaatcatga ttccaccagc ttcctttact ttggcgtctc atgtgccatt atttattgcg
     5161 atgtttttta caccaggagt tgcagtggct gttgcgctag gtacgacatt tggtttcttt
     5221 ttaacaacgc cggttatcat cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt
     5281 ggggcttggt acttgcaaaa gcatccgagt atcgttttaa aaaatggaca gtttactttt
     5341 gctaattggc gtttccaagg gttcaatttt gtgatcggtg tgattcattc tttagcagaa
     5401 atgctagtag tcagcatctt ttactttatt ggtaatatgc ctgaaacata ttattctcaa
     5461 ggatattttt atacagtatt catcttgatg gggattggtg ggcttatcca cagtttagtt
     5521 gattataata ttgcttattt tatagcagga acactcagca aatattttga tatccccgta
     5581 tttacggcag caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg
     5641 acatattaga catgatctct caaaaaaaaa actctcatct agtaatggat aataaatcct
     5701 ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg cacagctcta
     5761 acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa agaagtggcc
     5821 tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc
     5881 tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt cagctccaag
     5941 taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt caagggctac
     6001 ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc cttcataacc
     6061 tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc catgtccatc
     6121 tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag atccaataaa
     6181 gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac cgatttgttg
     6241 gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt ctgtctgatc
     6301 gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa tctcagcacg
     6361 catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta agaagacttt
     6421 tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac cagataaagc
     6481 agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat tgtccacacg
     6541 ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca tattatcaaa
     6601 aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat cacggtcttt
     6661 aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag agatatcttc
     6721 cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac caacaaaaac
     6781 gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat tttctgcatt
     6841 gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt ccacactttc
     6901 ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac ttcaatgtca
     6961 tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct ttaataaata
     7021 tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa tatcaaaagg
     7081 agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa
     7141 attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta ccgggacgac
     7201 tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat ccggaccgtt
     7261 aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt tggtgatttt
     7321 tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac ttatccgttt
     7381 atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg tgctactaag
     7441 aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag acaagcatcc
     7501 actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc ctatatgtag
     7561 ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg gataatataa
     7621 acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata gtttctgtgg
     7681 gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta agaggatggg
     7741 atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg
     7801 atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac
     7861 gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc cgactggtag
     7921 caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg atcatgggca
     7981 ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta gtaggaatgg
     8041 ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt attgaaggag
     8101 caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc attctggcta
     8161 cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt gcgttgatgt
     8221 accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa acagtaatat
     8281 ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct tacaaaaaaa
     8341 ttttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc atcccagaag
     8401 actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta ataaccatct
     8461 ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc catcactaat
     8521 atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact ggtttttcct
     8581 tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc gactgagctg
     8641 gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc aatcgtcaac
     8701 agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt atctaatttt
     8761 tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc attgatttct
     8821 gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact gtcaaatagc
     8881 caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg gatagagcgg
     8941 atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg catcagcaaa
     9001 ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt ttgacctgga
     9061 taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca
     9121 tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc atctggcaaa
     9181 tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg
     9241 gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa ctgacttgag
     9301 tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt caatgccaat
     9361 aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg agaagacggg
     9421 tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc agcatttagc
     9481 agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat tttctggtta
     9541 ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg tctatcgaaa
     9601 tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg tgtagaacaa
     9661 agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga aagaatgatg
     9721 attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc
     9781 atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata ataacgaagt
     9841 ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga taagtaagct
     9901 aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa aacgatacca
     9961 ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc tccaatcatt
    10021 tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc attgatgatt
    10081 cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg aatgaatcgg
    10141 cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc tgtgatgcga
    10201 cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc tgagcagcaa
    10261 tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg acacgttggg
    10321 agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa tccaagtctg
    10381 tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc ttggcaatca
    10441 aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc ccattttcca
    10501 tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc tgagcgattt
    10561 tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga aggttttcac
    10621 gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc tgacggagat
    10681 gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg gaactgacat
    10741 catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga
    10801 ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa agaccagaat
    10861 caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg
    10921 gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc tctttcaccc
    10981 gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg
    11041 ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt tgcaagctac
    11101 caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca ttcatgatca
    11161 aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa ttgttcgcta
    11221 gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca aatgcgcgga
    11281 tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct agtttttctt
    11341 gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg attgagaaaa
    11401 taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa gccatgacca
    11461 ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt gctaaagcta
    11521 gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat
    11581 tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt aactctgtac
    11641 caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa atcagaccaa
    11701 tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg tcattcattt
    11761 ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg aagacagctt
    11821 caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt gcataacgta
    11881 ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa aaacaagtac
    11941 taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt cataaatgaa
    12001 ag
//
LOCUS       DAZUKD010000083        11865 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000083 DAZUKD010000000
VERSION     DAZUKD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.085"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..755
                     /locus_tag="ACG8N9_001976"
     CDS             168..755
                     /locus_tag="ACG8N9_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897541.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            755..1654
                     /locus_tag="ACG8N9_001977"
     CDS             755..1654
                     /locus_tag="ACG8N9_001977"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4897542.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            1654..2568
                     /locus_tag="ACG8N9_001978"
     CDS             1654..2568
                     /locus_tag="ACG8N9_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897543.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            2620..2835
                     /locus_tag="ACG8N9_001979"
     CDS             2620..2835
                     /locus_tag="ACG8N9_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897544.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            3025..3279
                     /locus_tag="ACG8N9_001980"
     CDS             3025..3279
                     /locus_tag="ACG8N9_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897545.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            3458..4507
                     /locus_tag="ACG8N9_001981"
     CDS             3458..4507
                     /locus_tag="ACG8N9_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4897546.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKLLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVSGTYQGIMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            4682..4912
                     /locus_tag="ACG8N9_001982"
     CDS             4682..4912
                     /locus_tag="ACG8N9_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897547.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            4915..5172
                     /locus_tag="ACG8N9_001983"
     CDS             4915..5172
                     /locus_tag="ACG8N9_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897548.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            5389..6264
                     /locus_tag="ACG8N9_001984"
     CDS             5389..6264
                     /locus_tag="ACG8N9_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897549.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            6274..7068
                     /locus_tag="ACG8N9_001985"
     CDS             6274..7068
                     /locus_tag="ACG8N9_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897550.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNIL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            7367..8215
                     /locus_tag="ACG8N9_001986"
     CDS             7367..8215
                     /locus_tag="ACG8N9_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897551.1"
                     /translation="MIEYDFNEAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKV
                     RPYENLKTYMLFLVNFEGTAKEGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLI
                     LDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQK
                     SLREGETNTFDKYSMYNRAYSSMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRS
                     EVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKGFENKGSFGNSKDEVKGVVIDDD
                     FEFDFD"
     gene            8248..8568
                     /locus_tag="ACG8N9_001987"
     CDS             8248..8568
                     /locus_tag="ACG8N9_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897552.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            8823..9716
                     /locus_tag="ACG8N9_001988"
     CDS             8823..9716
                     /locus_tag="ACG8N9_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897553.1"
                     /translation="MRTHYIYLEDKIIDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            9736..11370
                     /locus_tag="ACG8N9_001989"
     CDS             9736..11370
                     /locus_tag="ACG8N9_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897554.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNNGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMSLTIKRIYDLLYVYKH"
     gene            11399..>11865
                     /locus_tag="ACG8N9_001990"
     CDS             11399..>11865
                     /locus_tag="ACG8N9_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897555.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRT"
BASE COUNT         4580 a         1467 c         2481 g         3337 t
ORIGIN      
        1 ggatttacaa aatgaataag tttgaagagt gatttggtcg cagactagat cactcttttt
       61 ctgtaaaaaa actttacact tatttttgag tgtgttaaga ttttttatat agagtgacgg
      121 tttcttgtca gagtgaatga taaccatcaa tattggagga ataataagtg gaaaacaata
      181 atgatattag atattttagc attggggaaa gagttacaac agtaagtgaa ttatcaaagg
      241 attatagaga taaagaagga acgattattt ctcttaaaaa gaatcgaaga ggtgtttggc
      301 aatatgttgt tgatttaggg gatggcgtta cagttgagtg taaagatagt caactgatca
      361 gaaatgatgg cagtcattcc attgaaaaag caattcttgt attcccaaaa gtatcaagca
      421 agattatgaa tgactggttg cttggaaaat ttggtgagta caataaaaaa gaaatcgcag
      481 aagtgatcgg caaatcttat ccaacagtat tagggtatct aaaaaagata acgaacaaag
      541 aagcgaaagt tgaggatgtt ttaacagtaa gagaacttca gtcattaatc gaatatcgga
      601 ataaacaaat aacaaatcct gagtacctta aaaaagaaga tggtctattg aaacaattgg
      661 aacaggtaaa taaaatccgg aaaatgcaaa aggtagctcc tttaagccta gataacttta
      721 aaaaaatcta tttgaaagag atcgaaaaag actaatgtgg agtgtcactt taccgtttca
      781 acctgtatct gcaccaagac caagtgcaag aaggaatgtt gctgatgcaa ctaatgacgt
      841 agcagagaaa aggatttcaa cctattatga taaaaagtat ctcgattatt tgaaaaaaat
      901 aaaaagcttt atcgaagatc aaggtttgtt agatgatgaa ttttactcga tcgtaaatga
      961 tccgatgggt gcaattatgg aagtggactt ttactatcag ataccaaaaa gctataaaaa
     1021 agtctataac attatgaaga cgacagcgcc agatttagat aatttagtaa aaagtgctat
     1081 ggatggtatt ttcaacatct cagctatacg agatagccat atcacagggt taattgcttt
     1141 taaatacaat gtggcaaacg aaggaaaaac agttgtgagg atcaagcgat attcagaatt
     1201 tgagtgggat acttctgaag gattgaacgg agatatttgg gaagccacat ttgatttcaa
     1261 accagtagca acgccaagac caaagtctaa atttagagga aatgggatta tcacttacta
     1321 tccaaaagag tataacgact atttggaaaa tatcaagaat acgcttaaag agaaagattt
     1381 ggtaaatgat caactgaaaa aggtaatgaa tgctcctatg ggggttatag ctcagataga
     1441 ttatttttac caagtgagaa aagataagaa aaagcttgat tcactgatgc gtacttcaca
     1501 gccggatatt gataacttag tcaaaggaac attggacgga atttttaaca aggaaattgg
     1561 catcaaagat agcagggtgg taggtttaat agcttttaaa tacaatactt ttgaaaagtc
     1621 gaaaacaaac gtgcgactac aggagatggg ctgatgaata ataacgaaaa aattcttcat
     1681 gtacttgata gctttgaaac aattcaagag gaactaaaga aatatcgaga tgttttggaa
     1741 caaagatatg attttgtcaa tcagcaaaag agtaatcaca tggatttcat tctcaatatg
     1801 aatgacttga agaagaaatt agttgaacga aaagaacaag aaaagttgat caaagcttac
     1861 tttgaactcg gtgaaaaaga agttaaaaag gcaatggaac tgaatgaaga tcgcagggtt
     1921 ttagatcagc tgttagaaca attattggtg atgtttcaaa aaggacgaat agatgaggat
     1981 ttaattgaag aagggttgag aaagtatcct gcaaactcag gtatcggaat agtgctgaaa
     2041 gcgattgatg aagaagaaat tgaagatttc ataccggcag aggattttga atcggcaatg
     2101 gaatatataa aatattacag ccaagggatc actgctttta gagagtttga tcctgaagat
     2161 gttattcatg acttgaacaa cctaaaagaa tggtgtgaag gttatgaagt tgatgattcc
     2221 ggtttggact acttgatcag tattatggaa atcgaagaag agatgccgga taagcctgat
     2281 ccaacagata ttttagaatt aatccatgat gctagaaatc caattgcata catttctaga
     2341 ggatatactg tattggaata ctataagcct tatatatcag cgatgaacca tttaagacgt
     2401 gtattaaggg aaaaacgtga ataccgctca gtgttaaatg catcgaatag attagaaaaa
     2461 gctgtttcag agctagatgc ttattatcgt gaacattacc tgcaagcagg tggtatgcca
     2521 agaaatacga aagcaaatat cagtaggtat ataaaagaag ctgagtaggc ggagttagcc
     2581 cataaaatgg caaaaggtaa cctgaaaggg gcgttttgaa tgaaaaggga tgtttttgaa
     2641 ttgtacgtat tagaacgtct agaagacggc atgatggcac aagagataat tgatgaaatc
     2701 gatgatatga tcagcagatt tggtcaattg gaattgaatg atggaaacaa aaattcggta
     2761 gtgctacaac aattttggtt aaataatttt tcgtataagg attttatcaa agaaaaggca
     2821 aatagattgt actgactaaa ttcacgagga tcagtgatga gagcttattg ctaagagcag
     2881 ttaggctctt ttttgtttgt gttaaattat tttattttac aatacacatt tatacacaaa
     2941 aaatgttgac ggatagtaca caaatgtggt aggatatatt tgtaagcaag ttagattaac
     3001 aagttaaagg aaggaagagc agttatgaaa aaagaagaac taatagatat gtttcaaatt
     3061 gttgaacgag caaataatat gggaattatg ttttttgatc gtatttcttt aaagatggat
     3121 ttaagcgttg cccaccaaga atttaatcta cgattaaaag ctttattgat ttctgatgat
     3181 gttaatttcg ctcatgatgt tgtaggtata cagaaccata tagatagaga aaacaaacgt
     3241 atgggtgatg gattcttacc aagatattca agtttatgat ttggcgaaag attcaagagt
     3301 atgtgtcatt attgataggt actcttgaat tagaaaggag gaatgaattt aagacaacaa
     3361 cgattatgat cttaaaacaa tcgtttatgt aattggcaat cttacgaata attttcatgc
     3421 aataatgaca ataaaaaatg aaaaggggta aaatcaaatg aaaaaatttt tagtaggaat
     3481 tctattcaca atggggctag tgctattatc accttcagtt tcagcagaaa gctctatttc
     3541 catgtatcgt atgtataatc ccaatagcgg agagcatcta tacactagaa gcgttggaga
     3601 aagagacaat ctgaaacgtt atggatggca ttacgaaggt gtcgcgtggc aagctccaac
     3661 aagtggttcc ccagtttatc gcttgtacaa cagatataac ggtgaacatt tttacaccgt
     3721 tgatgccgga gaaagagata agatttcgca cacaggatgg acgtacgaag gtgttgcgtt
     3781 ctattcttac aacggagcaa atagagttcc tgtgacacgc ttgtacaaca aatacgtgaa
     3841 ttggcaccat tacacgttag atcaaaatga aaagaacgta attagtaaac aaggttggaa
     3901 gattgaaggt acgggttggt atgcaatgcc aaacagcgca ccaaatccta cacctgcacc
     3961 tgctccaagt aatgagaaat tgctaaatgt tcctgtcatt tggcaaggta atacgatgtt
     4021 gtgtgaaggt gcttccctgt tagagggatt gcactacaaa ggagtagcga caaatcaaaa
     4081 cttaaccagt tttgtaaact atatgccaag agctaatgac aataatcctt atcatggcta
     4141 ttctggcgaa tggcgctaca atgtgagtgg gacgtaccaa ggcattatgc ctgatccagt
     4201 agttaaatgg gcaaaaaatg ttggtggtaa tgccgttaat atcactggtt caggaacgaa
     4261 tgggatcaag aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta
     4321 tgcttcacct caattcaaac aaatgccttg gggacgtgca gtctggaatg gacatgtggt
     4381 tctcgtggat ggcttcaaaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa
     4441 atggatcaat gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt
     4501 acgttaattg atagaattaa taaataaatg aaataagagc ttaattactg aaatggtaaa
     4561 tgggctctta ttttttagaa aaaacttttt ttgtaatacg aatctataac aaaaaacgtt
     4621 gaatatagta cacaaaagtg gtatattaaa accgtagaga ttatgcgaag aggtgatttt
     4681 gatgaaccaa agaagaagaa aaaggctaga agaaataagc gagcgactat ccgagttaaa
     4741 ggatgagtta gaagtaattc aagaagaaga ggaagagtac aaagaaaata tacctgaaaa
     4801 tctacaggga tcagaaagat atgaaaaagc agaagaatcc gttgatcaac ttgaagaagc
     4861 tgttgatggt ttatccgaca tattagaatt gattgaagat atagctagct agagatgatt
     4921 aatgaagatt tcaggagtga gcaaaaaatg attgatattt cactgataga gaagctactt
     4981 ttttcaaata agtacacagc caaagagctg gctacaagat ggcaaatatc cgaatcagct
     5041 attaaagcct atcgtcaaaa taagggaagc gtaggatggc gtgattggaa aggaatcagc
     5101 attggaaaag cagaagacat aatgaaacgt gaaggatttc aagctgacaa tgattctaaa
     5161 aaagttgaat agtttaatag tcgccaagaa aagtaccagg gcgaggggaa aggtagatta
     5221 caaaggaaaa cacttttaaa taaacttcgc aaaagcatca ggttatgagt ggctagggct
     5281 atacgaatgt gatgaaaaca aagggcttta atgtcaaaga aaacgcaata gggggggaat
     5341 aaaattttta aaaaagatat ttatgtttgg tttaatggga aaaaatatat gactactaac
     5401 aaaaggtggt cagagctagt acaaaataca gttaaaaaag tcatgccaga tggatatcat
     5461 ttcaactata tacaatgctc acaaacaact gaaagtattt atttttcaat attaaatgag
     5521 aatagactct attatttcag ggtaagttca caccatagga aaaagagaga agataatgtt
     5581 acgacatttt tatcgggaaa atacaatggc ttcaaagagt taagtgaagc aattggtgag
     5641 tatctaaaaa gtaatagatt tgtcagaatt tctgacaaac atatattgat cttgttactt
     5701 tatttgaata atagcttcaa attattttgg agagacgaca ctttctacta tattgagaac
     5761 ggcatggata tttattctgg tatcaacggc atattaaaac attatattaa ggcgttactt
     5821 cgatatgggg ttctttatca agaagattta agtaaccaaa ttaagattag taattctgga
     5881 aagtcattac tggcaaaata taaaaaattg ctttctccta tctacaaagg aatagatatg
     5941 gaagctgtaa attctgaaac tttatttaat ttagttgttg atgaaaagtt tgcgaatgaa
     6001 ttttttaaaa gtaaaaaaat tgaaagacta aataacctgt ttcctgaaaa tgcaataaaa
     6061 gcttatgtag ataataataa atcacgatta tacaactggt ttattcccaa acggttgcga
     6121 ggaaaaatca aaattggcga tcaagtgaaa gtaattaatc taaatcatac taagatcgtt
     6181 accgtaacgg agttgtatat agctgaaaaa gaagagatta caaaaatgaa accagttatt
     6241 aaaatactag ataacaaaga gtgagtggaa tatatggtag caaaaaagaa tactctaaaa
     6301 gaagcttctg aaaaatatca agatatgaaa agacgaggat taatcccaaa ggacaaaaaa
     6361 ggtatatgga gagaatttgt taaggaagtc agtagtgatc gaagtggcgg tgactttgtt
     6421 ggacgatata cagcaaaagg ttatagcagg cgcttaagga tcactgttga atatggaacg
     6481 aatgaatcag ctacagagct gaagcaaagg tacttagata cacgaagaga agcttcaaaa
     6541 aaatatgatg atttcaagag cagaggtcta attcctaaga agaaaaaggg gatttggcgt
     6601 gagtttttca aagaagaaga tagcaatcgg aataagggag acttcattgg acgatacaca
     6661 gcaaaaggct acactaagaa tttagggatc aaggttgaat acggaactgg tgaaagttca
     6721 tgggaattga ggaatcgcta tttaaatata ttgttagaga aatataaagc agaattattg
     6781 gagttgttcg atgttaatga ttttatcgag atggctgaaa agtatggtca tgaagaaggt
     6841 tttatgatca atgacattat ggttattgat aattttacgg atttattagc acttgcgaaa
     6901 aaagcaaggg atttaagaga aacgcagatg tatctattca gaaataaaaa cggaagaaac
     6961 cggagaactg aatcgagtga gattgaagat tactacgatg cctttacaca cgatctggga
     7021 gcctttgtag acgagataag tggtgtaatg aacaaatata gggtctagaa taaaaaagcc
     7081 ggagatgagc agataaatgg gaaacaatag catatacaca cctgaaatgc agaataaaat
     7141 taaaagactt gtcggattag ggtggatgac aagaggaaaa ttagattctt ttagtacgat
     7201 caaatatctt ttgatggaca tttaccgtca gaacaagcaa acaggaatac attttttggg
     7261 ctatgataag ccggaagatg cacctttctt tgttccaagt tcagcctata gagccagtac
     7321 ctccggtagc atgtacaact tggatttgtt cgaggaagga gcaaagatga tcgaatatga
     7381 tttcaacgaa gcctattcga ggatcatgag aaattatcct ttgccgtcaa acacttattt
     7441 gaaagcaaaa tataatacag atcaagctat tttgagagcg ataaatagac caaaagtaag
     7501 accgtatgaa aatttaaaaa cgtatatgtt atttttggtt aattttgaag gaacagccaa
     7561 agaaggaaca tatgctaatc caaaatcaca gtcgatgttt gcggattatg gtgaaaatcc
     7621 caaaacagct aatgagccta tttggttatc ggagattgag ctaacactta tccttgatta
     7681 ttatgatatt aaagagatgg aagtactaga tagccacaca tttttgacga aaaaaggatt
     7741 actcaaagaa tattttgaaa gaatcgagcc attaaaaact gatcctgata cgttgaagct
     7801 ttataaaaat cttagaacga aagtatttgg tgctatcgga caaaaatcat taagagaagg
     7861 agaaacaaat accttcgata aatacagtat gtataatcga gcgtattcct ccatggtagc
     7921 aggcgtattt agagatcgta tggttagata tgaacagaaa tacgttcata gcgcatatgg
     7981 gctattaaaa attcgtactg atggattgta tttcagatcc gaagtaccag agtttgaaga
     8041 gctgtataaa cttggagtag ttaagaaaaa agttcataca atatccaaag aagaagtcga
     8101 aaaagcttta gaatttaaaa acaaaggatt tgaaaataag ggctcctttg ggaactctaa
     8161 agatgaagtt aagggtgttg tgatagacga tgatttcgag tttgattttg actaaatact
     8221 ctttggaaaa gatggtagga ggtaaatatg aagaagaaaa ttgtggaagg aaatgtaggt
     8281 gttattattc taaaagattc aactgactat gctaaatcaa tggcactaaa agacgaaaaa
     8341 gttgcacgca tggataaaga tttagttgaa tttctgatgg atgatacaga aagattaaaa
     8401 ggggatcagt ttttgagttt aatgaaagac aaaggcttaa acacatatgg tttatttgat
     8461 tataagaaat tagctataga gtgggtaaaa gaaggagaaa tattttttat tgaagaatgg
     8521 gaaggtactg agaacttagt tcttcatcag tatcttttga aagcttaatc taagtaaaac
     8581 agttctgact agttatgctt gtcagaattt caaaggatgt tttccataac catcatcatt
     8641 caaaaataaa ggatttgaaa ataagggttc ctttgggaaa tctaaagatg aagttaaggg
     8701 tgttgtgata gacgatgatt tcaagtttga ttttgactag atttttttgc aaggtttaag
     8761 agtaaacaag gtaagaacta agatcatgaa gattgagagt aaagaagata gaggacgaat
     8821 gtatgagaac acactatatt tatttagaag ataaaattat agatgaaatt ccggcaaaaa
     8881 gtcgagaaca agctagaaaa ttatttattg aaaaatacaa agagatcaat ggagatagtg
     8941 aagttccaaa agtggtcgta acagttagaa aaaggcaaac aaaaaagaga agtacgtttc
     9001 atgtcagccc tgatatatat aatcttatag agcgacatag aagtgaagat aatatttcta
     9061 tctctcaata tattgctaca gttattgatg aattgatttc aggaaagtat gactatgctt
     9121 ttgattttga ggatgaaaag gattacaaaa gtgttgctgt ctttcaatct gattttgaga
     9181 agctaaaaaa ctatgctggc aggagaaatg ttaattttgc aagactacta gaagagttct
     9241 actccgaaga gttgggagta ggaaatgaat acaagagaga aacttatgcg aaattgtctc
     9301 ataagttatc ccaagcgctt aaagaagtga cagaagttaa agcggaacta gatgctaaac
     9361 ttgaagaagt gaaaaaatat caaacagaga ggaagaacaa catgaagaat caagtggaat
     9421 ttgacgaaca gaaagaattt gaaaaagcaa gaaaacgcta taacaaaatt atgaatgaac
     9481 ttagctatga acacttaaca gttcatgaag aattgagtga gggtacagaa aaatatacga
     9541 tcgcagatat ggttaatgaa gtggaaactc ttttagcttc tttctttgaa aatggtcacg
     9601 taaacgatga atggcgaaaa gaagaaccta aaagggttaa cgcttatatt gctcgattaa
     9661 aaaggtttat tgataaatat aaggagaata gtgacaaatt ttctaaagat aaataaggag
     9721 agagtgtgat tacatttgcc agaaatcgaa ggaatatttt cgccaaaaat aggtaaaaaa
     9781 cagttagatt tcagcagttt tccgagcgta aatagagatt ataatctagg tgatgaggta
     9841 cagatactaa atattgattt caaagaacta gacttcgctg ttgctgtttc caacaaaccc
     9901 aaggcattgg aacgagtgaa aaaaatatac caagagaatg atcaaaatat caaaaatatg
     9961 atcaaaaagt atagacgaga taattcaaaa gttgaagacg ttttattcaa cggtacggca
    10021 aaacatgatt tgaatgttta tatggcatta ggagtggctt tgtactgtaa ttcaattaat
    10081 cagacagata ttttggttaa tatcgtgagg aatagctatc aaaaaatatt aaaaataagt
    10141 agcgaaacaa acccttatta tgcaatgaaa tatcttgggg ataatttatt aaatgataaa
    10201 aaattgatgc acaacactga agagctgtta tctaatttgg ctgcgattaa ctttctattc
    10261 aaaacaggtt ctgaagatta ttttatagat aacgtactaa aaggtgctta cggaacagca
    10321 ttttatccgc tttacaatat gaaacagcat aaagccgaac aaaaggataa caaacttgaa
    10381 attaaaaaga tgaaagaact attaggtata ggatttgtaa agcccaattc aaaagtcact
    10441 attgcttcgt tagcaactac taacaataat gggaaaatag taaatgggtt tgacgattca
    10501 gatatagata tattgtcaaa atcatatttt tctttgtatg gagattttgc agatttgatg
    10561 ggtattgata gttacttagt caataatttt gagatcaagg gtagcgagct gaatgatatt
    10621 gtgtatttcg ttttaatctc tctatatgca attgatcaga ataccgagta ccgacaaatt
    10681 gactatttgc tagtttttta tagtttgtta tatcagttcg ttttaactcg gttgtttaaa
    10741 aatgctaagg aagaattgat cctgaattct gattatgaaa tagcaaaaca aattgcaagt
    10801 attagaaaag aaaacaaatc tgaagtaatc aaattaagaa aagaattaag tattgcgagt
    10861 aaagaaaacg gtcggttaga atctttagtc aaatcggcaa aaaaagaaga ggaacttttg
    10921 aagcaagaga ttaaagaatt aaaaggaatc agaaaagaac ttgaacagct gaggtttgaa
    10981 aataaagtat taaagcaatt gcaggagcaa gaacatagac aatcattaaa tggtaaaact
    11041 gaagagcctt cactgtctga aatgatagct tatctaaaac ataaaaacat tcttttagtg
    11101 ggaggacatg taaattttca aaataaatta actgaactat taccaaatat taaaatgttt
    11161 gatgtaagcg aaaataatgg aaagatactt aaaaatttga atacgtgtga tgcgattttt
    11221 ctatatacag attacttgaa tcatggtcta tattatcgtg ttatgagtga aatttctaat
    11281 aaagataaga tattttattt agataatcat gtgaatatga gcttaacaat taaacgtatt
    11341 tatgatcttt tatatgttta taaacattaa tgaggtgtta aaattgacaa caataactat
    11401 gacaaaaaaa atagaagtat atgttgatgg atcacaagaa gaaagggatt ccttttttga
    11461 ttttttttgg aaaaatagta gagaatatgt aaaagcgttg aatctggtat ataaacagct
    11521 aatgtttgaa gagttgataa aagatgcaat taaagaaatg gatcaaggtt atgctgagag
    11581 agaagaaagt attcaaaatc ggattgaaaa gttagcatat actgatccaa tagatcaaaa
    11641 aaaacttgat aaagcaaaag ctgaattatc taaattgaga aatgaaaaaa ataaagaagc
    11701 gagagaaata ttaacgcaag cgatagggtt gaagagtcaa acgagaacac gagatgtagc
    11761 aaaaaaatta gactttgact ttggagattt tgttgattcc gcaaatatga aagctagtaa
    11821 agattttggc aatgatttta tcggaattgt gaccggacaa agaac
//
LOCUS       DAZUKD010000084        11648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000084 DAZUKD010000000
VERSION     DAZUKD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.086"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..891
                     /gene="thiD"
                     /locus_tag="ACG8N9_001991"
     CDS             76..891
                     /gene="thiD"
                     /locus_tag="ACG8N9_001991"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4897556.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1096..2547)
                     /gene="cls"
                     /locus_tag="ACG8N9_001992"
     CDS             complement(1096..2547)
                     /gene="cls"
                     /locus_tag="ACG8N9_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4897557.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            2889..5555
                     /locus_tag="ACG8N9_001993"
     CDS             2889..5555
                     /locus_tag="ACG8N9_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4897558.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPVIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5919..6671
                     /locus_tag="ACG8N9_001994"
     CDS             5919..6671
                     /locus_tag="ACG8N9_001994"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4897559.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6693..7847)
                     /locus_tag="ACG8N9_001995"
     CDS             complement(6693..7847)
                     /locus_tag="ACG8N9_001995"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4897560.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8032..10473
                     /locus_tag="ACG8N9_001996"
     CDS             8032..10473
                     /locus_tag="ACG8N9_001996"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4897561.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(10803..11507)
                     /locus_tag="ACG8N9_001997"
     CDS             complement(10803..11507)
                     /locus_tag="ACG8N9_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897562.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3749 a         2082 c         2468 g         3349 t
ORIGIN      
        1 actttgaaaa acatatgata gaaacagtaa cagcattatg aaaaatcttt ttataaaaat
       61 ggaggaatcg atttgatgga agatcagtat gtacaagcaa tgacgatcgc aggatcagat
      121 agcgatggaa gcgcagggat gcaagcagat atgcatacat tctttacaag aaatgtttac
      181 ggtgtttctg tcatgaccgc atgtgttgca ggtaattctt acggaatcgg agcaagtgtg
      241 acattgccaa cagattttat cgacaaagaa tttgaactga ttgcgaagga ctttcaaata
      301 cgtgcagcta aaacaggtat gctagcagat tcaaagttga ttgagacagt tgtaaaaaat
      361 taccaaaaat atgattttgg accattagtc gtggatcccg taatcgtaac gaaacatgga
      421 aatcttttac tagaagaatc tgctttgcaa agcttgaaag aaaagcttgt ccctttggcc
      481 gaagtactga cacctaattt ttatgaagca gaaaagctga ctggacggac attagcagct
      541 gaagaagatt ttgtgcaagc agcaaaagaa ctgcaagata tgggtgcaaa aaacgttatg
      601 atcaaaggac atcatgctaa agatgcgcag caagaaacag tcaatgacta cgtattgctc
      661 gaatcaggaa aatcattttg gttaaatgca ccattttatc ctaccgaccg gatcaacgga
      721 actggcgatt cgctatctgc atgtattgca gctgagttag cgaaaggaaa aacagttgaa
      781 caagcgatcc gattggcaaa agactatgta aaccgtgcaa ttcgtgaaga aataaatgta
      841 gggcacaaat atggacccat caatcattgg gcagctggtg aattagagta aaagaaagag
      901 gtcgtgagcc tgccgtccag ctccgagtca caaggaacaa gttcaaaaaa cagcttctca
      961 tgtttgttga atattgtgac ttgtgaccga ggagttggca ggagaacacc gtttgttcgg
     1021 aagaagggac tgtgacaaga tttttgtcac agtcccttta atcttagtat atttttctct
     1081 acttgaataa aaaaattata ggattggaga aaataatcga gaaatttgtt gcttgaataa
     1141 tagccagtta gacatttcct tgactgtttc agttgtcatg gatgaacaat gtgtctcatc
     1201 ttctttgaac tgagtagcaa gttcttctaa aaactcagga tcgtaaatga cagcacttgt
     1261 ttcaaaattc aatcggtagc tacgaatatc ttgatttgct gaacctacca tgcagatttc
     1321 atcatccatt atcaatgttt ttgcatggag gaagccgttc tcatagacaa ggatttgtac
     1381 attttccttg atcagctgcc gagcataata ttgtgttgct cgataaataa aaggatgatc
     1441 cggtttgttg ggaatcatga ttttcacatc tacaccagag gcagttgcga tttttaaagc
     1501 agcaatgaca ctttcatcag gaacaaggta aggtgtctgt atccaaacac gttttttagc
     1561 agaagtaatc aatttgataa atgaaagctt gatttgttcc ctttcgttat tcggtccgct
     1621 tgaaacaagc tggatggatg tatcagcaag cttagattca tctcgatctg cttttttaaa
     1681 gaaataattc aaatgatagc cgaccttttt ttcttcaggt accgagacgt tccagtctgt
     1741 taagaagcga agctggagca aagaagaagc ggcaccgaaa atccgtatat gcgtatcgcg
     1801 ccaatagccg aatttttttg ttgtatttac atattgattg gcaatattga aaccaccggt
     1861 ataactaatc tttccatcga tcacaacgat ttttcggtga tcatggtaat tcaatcggaa
     1921 acgaagtaat gccttttgtg aggtaacaaa tgtatggaca tgtccaccat ttttgattag
     1981 acgattgaaa tcttttgctt ttgtaccatg agagccaaat gcatcatata atattcgaac
     2041 ttccacgcct tctgcggctt tttcttctaa aacatgtaag acttgctggc cgatattatc
     2101 tgtcacaaat gcgtaatatt cgatatggat cgaatgtttg gcttttttta gatcttgaag
     2161 cagtgaatcc aatttctctt gtccgtctgt gagaagagtg acagaattca ttcttgtcag
     2221 cggcatacga tttaatgaag agaagaagtc gacaaattgc tgtttgtctt tatcgcccat
     2281 gtcaggatcg taatgttcaa tggtatctcc tctaaaagac tgaaagtttt ctaattcttt
     2341 caagtcactt tgtcggagat agaatttttt cttatccgtt aatccacgtc cgaaaaataa
     2401 atataataca aagcccaccc caggaagggc aaacaatact aagagccatg cccaaatggc
     2461 tgctacatct cggggtttga tcaaaatagc caccaaagca ataagtgcat ttaatagata
     2521 aagggcggtt ataatactag ataccacaaa aatgttcctc cttacataca ataacattag
     2581 tatagcatga tagaagaaag aagcctacca aggtgaatat acttttcact cctttcatga
     2641 aaatgtaggt aaatcgggct aaagtagttg ctttttgtag gttaataagg taggatatta
     2701 gtacagttga atatctcctt aggggagtaa ccggcaacgt ttgttgcgat aatatcaaca
     2761 gtaagttaac caaatggttt tctggtattg tatgaaatgg tgagacctaa gacaaatgcg
     2821 tatttgtctt aggtctcttt ttttttcaga caggactagg gccaagatca aaaaatgggg
     2881 gatgaaattt ggaaaacaag gaacaagcac aacaagcggt ctataaagaa acgatcgact
     2941 cgttgatgca gcaaacgcag agctcaaccg atggattgag caccacgcaa gcgaaggaaa
     3001 aattagaaag agatggcttg aaccagctaa aagaaaagcc gaagaagcca acttggaaat
     3061 tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt gctgattgtc gcactgattc
     3121 agatggtcat gggtgactac atcgaattct tagttatttt tgcagtattg atgttgaatt
     3181 ctataatcag tgttgtccaa acaaaaaaag cagaaggttc tttagaagcg ttgaaaaacc
     3241 tttcagcgcc cgatgctcga gtgattcgtg atggtaagga aaaaacgatt ccggcaaacg
     3301 aacttgtggt aggagatatc gtgttgttag acgcaggcga ttatgtacct gctgatggac
     3361 gtttactaga tgcgggatca ttaaaagtag atgaaggtat gctgacagga gagtcgactt
     3421 ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc aatcggagat cgtacaaata
     3481 tggtattcag tggaacgatc gtaacttacg gacgcggtaa attcttagtg acttcaactg
     3541 ggaataatac ggagatcgga caagttgctg gccttttgga atcaacaact gagcaagtca
     3601 ctccgttaca acgaggtctg gataaattca gtaaaaaact aagtctggct attttggcat
     3661 tgtctttatt gattttaggt atccaactgt ttcgtattta tcttggagaa ggtacaggag
     3721 atatgactgc agatatcgtc agcgcagtga tgtttgccgt agccgtagcc gtagctgcta
     3781 ttccagaagc attacagtct atcgtaacaa tcgtgttatc tttgggaacg aataaaatgg
     3841 caaaacgtca tgccattatt cggaagcttc cagcagttga aacacttggt tcgacaagta
     3901 tcatctgtac agataagact ggtacattga cacaaaacaa aatgacagtt gttgactact
     3961 ttttaggtaa tggcgatact ggggatttca caaatgaccc ttcaaaatgg acagctgacg
     4021 aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc atcgatcagt gaagacggta
     4081 ctaaattggg cgaccctacg gaagtagcgt ttatcgactt tagtgaaaaa ttaaaccagc
     4141 cttatcagga aatccgaaac aactacccaa gacaagcaga acttcctttc gattctgatc
     4201 gaaaattaat gtctactgga cacacgatcg ataacgaaaa ttacttgttt gtcaaaggcg
     4261 gaccagttat cgtcttccaa cgcagtaaaa aagtattgct aaatggtgaa gtcgttccga
     4321 tgacggatga attgatgaaa aaattccaag agcaaaatga agctttttca acacgcgcat
     4381 tacgtgtact agcttttgct taccgtccac ttgataattt tgatttatca tttgaagatg
     4441 aagacgattt gatccttgtt ggtttgcttg ctatgatcga tcctccacgt gctgaagtga
     4501 ctcaggcggt aacagaagca agaagtgcag gaatcaaaac aatcatgatt actggtgacc
     4561 acaaaacaac agctgttgct atcgcaaaac aaatcgggat ttttgaagaa ggaaacctgg
     4621 ctttgaccgg gacggaacta gatgaattga cagaagatca actaaacgaa gttttagaaa
     4681 aagtcactgt ttatgcacgt gtttcacctg agaataaaat tcgtatcgtt cgtgcttggc
     4741 aaaataaagg tcatatctca gctatgacag gtgatggtgt aaatgacgca ccagcattga
     4801 aacaagcaga catcggaatt gctatgggaa cgggtacaga tgtggcaaaa gatgcagcag
     4861 cgatggtatt gacggatgat aactttgctt ctattgtcag cgcagtagaa gtaggacgta
     4921 atgtgtttga taatatcaag aaagctattt cttatctatt tgcaggaaat ttaggtgcga
     4981 tcattgcgat catctttgcc ttagttgctg actggtccaa tccatttaca gcattacaac
     5041 tgttgttcat caatttagtc aatgattcac tgccagcaat tgcattaggt acagaaaaag
     5101 ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa ttcaggaatc tttacggaca
     5161 aaacattgat ttctgtaacg tatcgtggtg tgctgattgg gattgctgtt atctttgctc
     5221 aatggcttgg catgcagcat tcacctgaat tcggtgtagc catgtcattt tcaacattga
     5281 tttggtgtcg gacattgcaa acattcccag cacgttcaaa cacacaaaca gctatccaag
     5341 cagggttctt ctctaacaga agtgtcttac tagctgtagt tgtatgtagc gcgctttact
     5401 gcttaacaat gatcccagga ctgaaagaag tcttctctat cccatcagca tttggattga
     5461 cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat tgttttgatg gaaatcacta
     5521 aattgatcat tgtacgagta caaggaaata aataaaacga atcaacgagg ttgtgagcct
     5581 gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac agcttctcat gtttgttgaa
     5641 tattgtgact tgtgaccgaa gagttggcag gagaacaccg tttaaggaag aagttgtaaa
     5701 atcagcattt tgagctaata ctgagggtca tctgattgga cactgtttat tcggaaagag
     5761 ggactttgac acgactttgg tcacagtccc tttttttatt tatgatagac ggaaagccaa
     5821 tcattttatt attcatcaaa agagatagaa tagataaaag aggagaataa tttattccga
     5881 aaagtgaaaa catataacaa aaaattagag gaaatcaaat gaatgaaaaa gaatgggatg
     5941 aatttgctga agaatattat gaaatccagc aagaatcgca agtaactatt gctgaagatg
     6001 taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga aacagcagca gacgttgctg
     6061 gaggatctgg cagatttctt tccttagcaa aagaaattaa tcgctatgat ttgatcgatt
     6121 tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga aaaaaatgag ttgccaaatg
     6181 tttcctttat caaacagtcg ttagaagaat ttatccaatc aaatgagaca tatgaaatgg
     6241 tttttacagc agcaaatcca gcattggtac atcttattca attagaaaaa ttgatgaaaa
     6301 agtcaagcaa atggtgcgtg ataatcaggg tagtggaatc aatcgatgat gttttttatc
     6361 ctatcgaaaa gcaactagga atgatagaag aagatcctaa tacatcgcca gttgtaatgg
     6421 atcaatttga aacatactta attgatgaag gattcaaagt gaagaaaaaa gattttactt
     6481 attttacaca ggaatcaatt actgaagaat tgatgaagct atactatgac gcgtacgcaa
     6541 aagatcctgt tgtgaccagg tacattcgca aactattcag tgaaaaacga gaaatcctta
     6601 gtacgactaa gctgacctat agactgctcc tttggtccgc agataaaaaa agaacctttc
     6661 acgactcgtg aagggttctt tttatgattt tattatattt tgtagtaacg aatatcattt
     6721 tcgatctttg taatcgaaaa aggtacatct gaagtatatt ctgcacattc tccgtgttca
     6781 ggaagcgtct cactgaaaat cgtttcatat tcgtcaacag tgatccgtgt tcgagaatct
     6841 agcatctctt gatgagattg tgcgaagaga tattcttgat agttttcttc cagataacct
     6901 gtaaaaaatt cacttactgc accagaaccg tagctgaata gaccgatgcg atctcctggc
     6961 tgtagtgatt tcgaattttc cagtagagaa gttagcccca ggtataatga accagtgtaa
     7021 agattaccga ttcgtcggct gtaacggatg ctttcttcat agcgagccat aagacgttcc
     7081 tgattttctt cgtctgtttg gtctaatacg ctttgcaatg ccttttttcc catcttagta
     7141 tacggaatat ggaaagcaat cgcctgataa tcttcgagtc ctcgacccga caattcttta
     7201 tgtcgattcc aaactttttg gaatgattcg atatacgtag aattagataa aggaccatca
     7261 acaacaggaa attcgctata atctggacgc cagaaatcat agatatcttc tgtcagaaat
     7321 acgctgtcgt cttcaatcga taaaatacgc gggttttgag tgatcatcat cgcaacagca
     7381 ccgacacctt gcgtcacttc accaccgctt gccaagccgt aacgagcaat atcacttgct
     7441 atgactagta cttttcgttc tggatgattt ttgacatatt ctttcgccat gtgcaaagct
     7501 gcggtgccac cgtagcaagc ctcttttact tcaaaagaac gagcaaaaga ttggattttc
     7561 agtaaatggt ggataattac cgcactcgct tttgaatgat caatcccgga ttctgttccg
     7621 acgatgacca tgtcgattag ctcacggtct ttttctgtta cgattttgct ggcagcattt
     7681 gctcctagtg taatgatgtc ttcatttgca cgattgactg ccatttggtc ttgaccaatc
     7741 cctatatggt attttgcagg atcatcccca cggctttctg ccaactctgt catatctaaa
     7801 tataaattag gaataaaaaa ggaaagacga tcaatcccta ttttcatttc aacacctctt
     7861 tacttatgat aaaaaactta ctttactaag gtatcataaa aagattaaaa gaaatataaa
     7921 aaagagaata tttaaggatt gttaaaattt aatcttagaa aaaaacaact ggaacaagag
     7981 ctatttgttt ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa
     8041 gtcgttatga tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca
     8101 tttactgcag ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg
     8161 aaaaaagata aaatcgatca agtaatattc ggaaatgtcc tgcaagctgg taatggtcaa
     8221 aatgttgcca gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg
     8281 atcaatgaag tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa
     8341 ttaggtgaag cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg
     8401 ctgaaaccgt atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac
     8461 gacggattga ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaaggttgca
     8521 acacaatttt ctgtgagcag agaagaacag gatcgctatg ccttgtcgtc ccagttgaaa
     8581 gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct
     8641 gatgaagacg tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg
     8701 ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttcgcca
     8761 ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat
     8821 ctgccttatt tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg
     8881 ggaatcgctc caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca
     8941 acaatcgatc tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa
     9001 gaactgcagt tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat
     9061 ccaattggtg caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa
     9121 cagaagagat acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg
     9181 ttagaagcga atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt
     9241 acgccatctg aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg
     9301 ttgatttttc aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa
     9361 gtgagtgaag tggaaatccc aatgggcatc gcgcagaatt ttcaaatcaa tggtaagaaa
     9421 aaatggattc cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca
     9481 aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt
     9541 ctatcaggaa aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag
     9601 ctgattcttt gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa
     9661 gatatctcta ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta
     9721 gtggatgtaa aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca
     9781 aacaagttac gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt
     9841 gctacagaat cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac
     9901 aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt
     9961 gacccttacc gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg
    10021 gcagctacag gaaatgatac acgtgcggtt agcgcttcga ttcacgccta cgcggcaaga
    10081 aatggattat accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa
    10141 ctaacggtgc ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca
    10201 aaagcatctt tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct
    10261 gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa
    10321 aaaggccaca tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag
    10381 gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct
    10441 atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag
    10501 caagatttac agttatttta tttactgcaa atcttgcttt tttctctatc agaaaatatg
    10561 tttattatta gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa
    10621 gatatcttca ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga
    10681 caggtcaagc tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa
    10741 attattatat gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat
    10801 ttttatcgat cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat
    10861 ttgactttgt atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata
    10921 gaaatagttg ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt
    10981 tcattttgtt ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa
    11041 cttaaagcgg cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt
    11101 tccgtttttt cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc
    11161 gcagttggtt catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga
    11221 agattgtcac ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca
    11281 gtattgaagt tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct
    11341 aatccgatcg ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca
    11401 tagcgtcgca agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat
    11461 gaaggacgga attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt
    11521 cgctcatacc ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa
    11581 aacttgcgta tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga
    11641 aaaataaa
//
LOCUS       DAZUKD010000085        11526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000085 DAZUKD010000000
VERSION     DAZUKD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.087"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8N9_001998"
     CDS             complement(105..629)
                     /locus_tag="ACG8N9_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4897563.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8N9_001999"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8N9_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4897564.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8N9_002000"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8N9_002000"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4897565.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8N9_002001"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8N9_002001"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4897566.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8N9_002002"
     CDS             3157..4026
                     /locus_tag="ACG8N9_002002"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4897567.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8N9_002003"
     CDS             4052..4567
                     /locus_tag="ACG8N9_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4897568.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8N9_002004"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8N9_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4897569.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8N9_002005"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8N9_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897570.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8N9_002006"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8N9_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897571.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8N9_002007"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8N9_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4897572.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8N9_002008"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8N9_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4897573.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACG8N9_002009"
     CDS             complement(7736..9013)
                     /locus_tag="ACG8N9_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897574.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACG8N9_002010"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACG8N9_002010"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4897575.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACG8N9_002011"
     CDS             complement(9935..10879)
                     /locus_tag="ACG8N9_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4897576.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..>11526)
                     /locus_tag="ACG8N9_002012"
     CDS             complement(10881..>11526)
                     /locus_tag="ACG8N9_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4897577.1"
                     /translation="LGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFVNNRG
                     MAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNVSFGG
                     VAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGGAGAL
                     LVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ"
BASE COUNT         3272 a         2448 c         2038 g         3768 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgaga
//
LOCUS       DAZUKD010000086        11496 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000086 DAZUKD010000000
VERSION     DAZUKD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.088"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..2122)
                     /gene="glyS"
                     /locus_tag="ACG8N9_002013"
     CDS             complement(47..2122)
                     /gene="glyS"
                     /locus_tag="ACG8N9_002013"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4897578.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGKASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2124..3041)
                     /gene="glyQ"
                     /locus_tag="ACG8N9_002014"
     CDS             complement(2124..3041)
                     /gene="glyQ"
                     /locus_tag="ACG8N9_002014"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4897579.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3425..4237)
                     /gene="recO"
                     /locus_tag="ACG8N9_002015"
     CDS             complement(3425..4237)
                     /gene="recO"
                     /locus_tag="ACG8N9_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4897580.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4380..5279)
                     /gene="era"
                     /locus_tag="ACG8N9_002016"
     CDS             complement(4380..5279)
                     /gene="era"
                     /locus_tag="ACG8N9_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4897581.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFTQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5294..5689)
                     /locus_tag="ACG8N9_002017"
     CDS             complement(5294..5689)
                     /locus_tag="ACG8N9_002017"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4897582.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5673..6149)
                     /gene="ybeY"
                     /locus_tag="ACG8N9_002018"
     CDS             complement(5673..6149)
                     /gene="ybeY"
                     /locus_tag="ACG8N9_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4897583.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6166..8358)
                     /locus_tag="ACG8N9_002019"
     CDS             complement(6166..8358)
                     /locus_tag="ACG8N9_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HFS4897584.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDKNIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8382..9353)
                     /locus_tag="ACG8N9_002020"
     CDS             complement(8382..9353)
                     /locus_tag="ACG8N9_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4897585.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10059..11438
                     /gene="brnQ"
                     /locus_tag="ACG8N9_002021"
     CDS             10059..11438
                     /gene="brnQ"
                     /locus_tag="ACG8N9_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4897586.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3091 a         2419 c         2110 g         3876 t
ORIGIN      
        1 atagcctatt ttgtgcctgt tctctggttt atttacatat tagaaattat tttgtgatga
       61 tttgatctaa acttgctagt gacaaagcca tggcagcaat ttgacttaat tgcgttaatc
      121 ggttcttacg tactgctggt tcttctgcca tgaccatcgt ctgatcaaaa taattttcga
      181 tcagtgggcg aaggcttaca agctgatcat aattttcgtt gatcgtttgg tcagcaaagc
      241 ttgttttcat tgcctctact gctttgtgca gttctttctc cgcttcgttt tcgaacagtt
      301 cagggtctac ttctgattgt ttttcaagct ccgctttttt cgctaagttc acgacacgtg
      361 tcaaagcttc catcgatggt ttgaagtctt catctgcaag atgctgcttg aacaattgtg
      421 ctgctgcaaa tacttttgtc agatcttttt gttctgcgtt caaaacagct tcgatgacat
      481 catgacgaat attcttagtt gtcaataatt ggcgcagacg gcctttgata aagtcgatca
      541 cctgttgttg tcccgctgac aagccaatgc caaaacgatc cgtatcttta ctgataactt
      601 cactaatcgt ttcttgcaga acagacaaag gaaaggccca ttttttcgat tcgatgattc
      661 gaacgacacc atatgcttga cggcgcaaag catatggatc gtttgagcca cttgggatca
      721 tcccaactgc aaagaaagaa aagatactat ctaatttatc agcaatcgct aaaacagaac
      781 ctactgtagt ctcaggtaga tctccttcac tggatatagg catgtaatgt tcacggatcg
      841 ctgttgctac ggctggtttc tcgcctttaa gcagagcata tttttctccc atgatccctt
      901 gtagttcagg gaattctcct accatgttgg ttactagatc aaatttatag atttcagcag
      961 cgcgttgcag atccatcaat tcttcctcag atagtcctac ttgttcgcct atgatgcgag
     1021 cgatgacttg tacccgttgc attttctcat agatgctacc gattttttca tggaaagtaa
     1081 cagtttttaa tttttcaacg tattggtcga ttgaagcttt cttgtcttca ttataaaaga
     1141 attccgcatc ttctaaacga gcagtcaata ctttttgatt gcctcgtacc acattttcca
     1201 attttacccg atcgccatta cgagcagaaa taaaatgagg aagcaacata ccttgatcgt
     1261 tccgtacttc gaaataacgt tgatgttctt tcatagaagt gactagtact tcttcaggaa
     1321 cagatagata tttttcttcg aagtcgccag aaaagacagt tggatattcg actagattgt
     1381 tgacttcttc tagtaaatct tcatctagat caatgatcca gtgatttttt tctgctaatt
     1441 cgtttgcttg tgtgatgatc atttcttttc ttttttgcgg tgaagcaata acaaattgtt
     1501 catttaattt ttcttcgtat tcttttgcat gcgtaaatgt tacatcttcg cctaagaaac
     1561 gatgacctcg agaggctttg cctgttttga catccaagat ttcgaatggg attacttcgt
     1621 catctaacaa tgccacgatc cagtgaatag ggcggatata ttcaaaatcg tattctgccc
     1681 aatgcatcgt tactgggaaa gtgaggcttt taatgacatc tgctaaacct gtcaatactt
     1741 cttttgcttc tttgccgtga gtgtatttcg ttacatagac atattccacg ccttttaatt
     1801 ctctaaaagt gatagcatct gtcgtcagac cttgtcctcg gacaaatcct tcagctgctt
     1861 tcgtccagtt tccatctgcg tccaaagcaa tttttttggc tggtcctttg acttcttctt
     1921 cgctgtccgc ttgtcgttct ggaatatttg ttactttgat tgctaaacgg cgcggtgtgg
     1981 aaaatgtttc gatcgtatca taagtcaatt ggttttcatc taaaaatttt gctgtttttt
     2041 cttctaattg tttcatgctt ggtgtgacaa catgagcagg catttcttcc agcccgattt
     2101 caagtaataa gtcttttgcc atcttattca gcctccttca tgttttctgg atttgctgtg
     2161 tttttattca ataaagggaa tcctaatttt tcgcgttctg ccacaaagat tttggctaca
     2221 gcacgagcca tgttgcggat acgggaaaga taacctgctc gttctgttac ggatactgca
     2281 cctcgggcat ctaataagtt gaatgtatgg ctgcatttca aaatataatc atacgctgga
     2341 tgaacaagat tttcatcgat acagcgttta gcttcttttt caaaacggtc aaaattactt
     2401 agtagcaagt cttggtcgct tacttcaaaa gaatatttgg aatgttcata ttctggttgt
     2461 ttgaagatct cgccatattt cacgccatca gcccattcca gatcatagac actttcaact
     2521 tcttgaatat atgaagcaag acgttctagt ccataagtga tctcagaagt aactggttta
     2581 caaggtagtc ctccgacttg ctggaagtac gtgaattgtg tgatctccat cccatcaagc
     2641 catacttccc aacccacacc ggcacacccc atcgatgggt tctcccagtt gtcttcaaca
     2701 aaacgaatat cgtgttctaa tggatcaatt cctaaaagac gcaagctttc taaataaagc
     2761 tcttggatat tttcaggtga aggtttcatt acgacttgaa attgatgatg ctgatacaaa
     2821 cggtttgggt tttctccata acgcccgtca gctggtcggc gggatggctc tacataagca
     2881 gcattccatg gttccggtcc gattgcccgt aagaatgtat atgggctcat cgtacctgct
     2941 cctttttctg tatcataagc ttgcatcagc atgcatccgt ttgatgacca aaatttttgt
     3001 aatgttaaaa tcatatcttg gacggtcaat tttttactca tctgttttgc ccctttcaat
     3061 ttttaagcgt tgctgtcaat aagaatacag gaaagaaaac gacaaaaagt ccctatgtct
     3121 gattacagac atagggacga ataatttcgc ggttccaccc tacttccgaa taaagattcg
     3181 gcagcttcat ttatcgtact cgcaagtgcc tcttcaaaaa tcgtttgggt ccggcttcca
     3241 ctctcccgaa ctcgctaaac cacgtgtttt ttactgatct tggtcaatgt acgtattcat
     3301 tttgcagttt tcttgtttgt ataatagatc ctctcagtga acaatctatt taatctacgg
     3361 ttaataatag acggaagtcc ctgtatttgt caaatagttt tgattttttt gttgtatctt
     3421 tcatttattc ttctttttca ggtatctctt tttcaggcat gtttttctct ggcgtcttta
     3481 atacgtgtgc ccaagacttc atttgatcaa tgaacttttt gctttttaga tgaataccga
     3541 catattcatc atacaactgg tcaatgaggt cacggattgc ctgcttcgtt tcttctttca
     3601 agtggattcc actgatctta tcataagaaa tagccgaaaa cattcgaacg aaataaacag
     3661 cgcgaggatc tgcatgataa cggtgttcgt ccattggcca gtgtttctcg catatcacac
     3721 cgccatattt ggaagaatag tcaaattttc cttttgtttc cccgcatacg acacagtgcg
     3781 tccaaattgg cgcgattccg aagcgttgca gtatttgaac ttcaaagata ttcgtgatga
     3841 tttctgcatc atctccttga tccatccgtt gcagagcttg ttgcgtaaat tggaataaat
     3901 gcgggtcata ttgatgatct tcgattgctg catctactag atttagcaaa taggtaccat
     3961 aaccgctcaa aaagatatct tcttggatgt tccgaaacgc atggacgtct ttgacactgc
     4021 ttaaaaacga caaaccttca cttcgaaaat taccgatata gactgcttcg gtaaagggga
     4081 gcaaagccgg agctaatggc tgattttgcc gatggattcc tttgatataa aacatcattt
     4141 tgccagaaga ttccgtaaag attttgacta atttatcttt ttctttgtaa ttacggctag
     4201 ataaaatcaa gcctttcgat tctgcaagct ggctcatatt ctccctccta aaaaataaat
     4261 ctggaaaagc agattctgcc gatccagatt atttatgttt cgatcttccg ccgtttttct
     4321 caacgtgcgg atttttatcc agtttatttc tatacttaac ctataagaag ttcagaatat
     4381 cagtattcat cttttcggta gccgaagtct tgcagataga cttttttgtc tcgccagtct
     4441 ttttggactt tgacccaaag ttctagaaat actttgtctc ctaaaagatt ttcgatatcc
     4501 tttcttgctt tcgttcctac ttgcttcagc atttttccgc ctttgccgat aatgattcct
     4561 ttttggctat ctctttcgac gatgatcgtg gcctggatat ggattttatc attctcgttt
     4621 cgcttcatcg aatcaacaac gactgcgaca gaatgaggca cttcgtctcg tgttaaaaac
     4681 aagactttct cacggatcaa ttcagaaacg atgaagtatt caggatgatc agtcacttga
     4741 tcatctggaa aatattgtgg gccctctggc atttcactga ctaatacatc cattaagcgt
     4801 tcgacattgt ttccttctgt agcagagatc ggaatcactt gtgtaaaagg catctgcgtc
     4861 gaataatctt cgatgattcc cagcaactcg tctggatgga tcttatcgat tttattgata
     4921 atcagataaa ctggagaatg cacgtttttt agacgttcaa taataaaatc gtctccccga
     4981 ccgcgttttt gatcagcact gatcataaat aagactgcat ccacttcttt caacgcactg
     5041 taagctgctt ctaccataaa atctcctaga cgatgctttg gtttatggat tcctggtgta
     5101 tcgataaaaa tcagctgagc ttctggtgta gtatatactc cttggatttt atttctagtt
     5161 gtctgtgcct tgtcactcat aatggcaatt ttttgaccca cgattcggtt caataatgtg
     5221 gattttccta cattcgggcg gccgacgatt gcgacaaacc ctgatttatg ttctggcata
     5281 tacgttctcc tttttaatta aacaacaaat gatatatttt agggacaaat aaaatcaacc
     5341 caatgatgac agcaaaacaa gcggtcacta aaactgctcc agctgccata tctttgattt
     5401 ttttcccaat cggatgaaaa tgaaaatccg taaacatgtc cactacattt tcaaaaacag
     5461 tattgatgat ctctactatc caaacgagaa aaacagctaa aagcagccat aaccactcta
     5521 tccgatcaag ctggaaaatc acgcccataa ataaagcaag gataccgaac gcaacatgtt
     5581 tcctcatatt gcgttcttcc tcaaaaacgg ttttgactcc ttggacagca aattccacag
     5641 acatcaggaa atgtttgttt ttctctacgt ttttatcttt tgagtccata ggcatccaag
     5701 atctctttct gtaaaccaaa catttctttc tcatcttctg gtgtcatatg gtcatacccg
     5761 ttgatatgca agaatccatg taccgctaaa aagccaagtt cccgatcata tgaatggccg
     5821 taagcttctg cttgttcagc agcacgttca atagagatca tgatgtctcc tagtacacga
     5881 ggcaatggat tctctccatc ttcaaaaatc actgggattt cgtcttcgcc ttcttcttcc
     5941 agagcaaaac tgatgacgtc tgtcggtgca tcttttccac ggtaatcacg attgattact
     6001 tggattgccg cattgtccat gaaagtaaca gacatttctg tttcctctgg cagctttaaa
     6061 taagatgctg caaattgcag caagtcatcg atttctttca ttttctcttc tgagacttgg
     6121 tttgtttcat ccataaaggt aatatccatt atacatcgct tctttctatt cttcttgttc
     6181 ttcctttatc ttctcaactt ctgttttcat tttaggatat ttgatccgag aatggaaggt
     6241 gctacttaaa ccgctgatca atgatttttc cacttttcgg atttctttca aggtcagtcc
     6301 actatcgtct aattgaccat cggaaatacg ctcttcgata agattatgga caaaactttc
     6361 gattttttcg ctcgtcggat gatccattgc ccgtactgca gcctcacaac tgtcagcaat
     6421 attgacgatc cctgcttcac gactttgcgg ttttggtcca gggtaacgga attgctcttc
     6481 tgttacttcc ggattgcgtt ctttcgcttt gatgtagaaa taacgcatta gcgtggttcc
     6541 atgatgctgt ctgcaaatat cgatcaccat ttgtggcata ttataatctt ccaatatttt
     6601 ggctccatct gtcacatgtc caaagataat atgtttactg tcttctggca ataagaaatt
     6661 gtgcgggttt tccgctcctg aaggcaagtt ctcaacgaaa aagctagcat gtttgatctt
     6721 gccgatatca tgataatagc aagctactcg agttagcaaa gaacgtccgc cgatttctgc
     6781 tacagcgtta gcacttagat tagctaccat catactatga tgataagtac ctggcgcttc
     6841 ttccagcaac tgtttcaata aaggatgatt tggattgctg agttcattta atacaattac
     6901 actgtcatca ttgaccatca attcaatata tgggtgtaga ccgatcgtca gcaagaatga
     6961 gaagagtgca ccagtgaacc cgcagacgag cattaaccaa gtcgttgcat caccaaagct
     7021 cattccttga tagacgatca aaatgacatc catcatcaca ggaaaaataa tgacccacat
     7081 ggcagcagat aatccttgtt ctgagatccg tttacgtctg acaatcgttg ccatgagccc
     7141 agagaaaaca taagacatca agataatcgt tagaaagttc gtgccaatcg aatcataaaa
     7201 aatgaaaagt gcagatactg cttggaaaat accagccatg atccctgctc ttcgattcaa
     7261 gaaaaagctt aaaacaagtg gtgcaaaagc agcgggataa aacagcggga tataagtagc
     7321 ttgttccgtt tggaataatt ggaaaaattt catcagcact atgctgactg acatagctgt
     7381 cacatagaaa agcacgtatt ttgttctttc tcctgcattt tggaattgaa gtgaattata
     7441 aatcagtacg ccgatttgaa gcaaaacagc taaaaccatg gcaattagcg gaaaaatcga
     7501 agttgtttga ttagtcagtc ctagtaattc caattttttc atagctgaag catcgatttg
     7561 attaccttca cgaacaatga tttctccttg gaagatcatc actggttgta cagcttctcg
     7621 tgcggattgt ttcaattctt ctgtcttttt ctcattcaat acgtcattca ctacgatacc
     7681 ttgatccact aagtagcgaa tcatttgttg ttgtgctggt gtgacattca aatattggat
     7741 ttgctcttct gcttcttgtt taaaagcttc caattcactt tcacgaactt gtttgctcat
     7801 ctgttcgtca atgagcttca gactctcatc cctcacagta tggatctcag tttcagtcat
     7861 ttcaaatatc gtcgaataaa aattgttcgg tagtttttga taaaacgcaa cattttctgc
     7921 attcacattt tcaaaatttt tcttcaacgc ggcgatccgc tcttctgtag taggctttgg
     7981 gatattttta tcttcctttg ccttatcaat cttttcgtca taagatttgt tgatggcatc
     8041 attcgtctta tcgatcaatt caaatagttg cttgatccga ttattctggt catctgcaag
     8101 atctttttgg taagtgtatt ctggagtgac tgcttctgct gccagttttc ttttttgttc
     8161 ggtttcttcc gtgttctcga tcgttttgtt tgcacggata ctttcttctg ctacttggcc
     8221 ttctctgaaa tcattggcct tttgtttgac actactaaat gtaacgccgc agataatgat
     8281 aaaaaacaag aacagcagga aaggcaatgc tgcctttcca agtttttctt gtagtttctg
     8341 cagtgtttta ttcaacatgt gcttatcctc ctaagcaaag cttattcttg atggagatct
     8401 gccttttcat aagctttgat gatttcggca actacagggt gccgaacaac gtcacttgct
     8461 tcaaaattaa caaaatcgat tttcttgatt tgctttaatg ttcgttctgc atgaacaaga
     8521 ccacttgttg tccctcgagg cagatcaatc tgactcgtat ctccattgac aatcattttt
     8581 gattgataac caagacgtgt caggaacatt ttcatctgag caactgtcgt gttctgtgct
     8641 tcatccaaaa taacgaatgc atcatccaat gtccgccccc gcatgtaagc taaaggagca
     8701 atttcgatca cgcctcgttc catcaatcgg ttcgtatgat ctaatccaaa aatttggtat
     8761 aaagcatcat aaacaggacg cagataagga tctacttttt cttttagatc cccgggaaga
     8821 aagcctagat tttcccctgc ttccacagca ggccgcgtca aaatgatttt ttgcacttca
     8881 ccttttttca aggcagcgat tgctaatact actgcaagaa aagtttttcc tgtcccggct
     8941 ggtcctacgc caaatacgat atcatgtttg gctaccgatt cgacatattt cttttggccg
     9001 ctgtttttta cccgtatagg ttttccatta cggtctttga cgatctcttc ctcatacatc
     9061 tcaacaaaat aatctaatgt gcctttttgc cccattttca gtgccgtcac gacatctggt
     9121 gtacttacat ggattcctcg tttgatcaat tcctgcaatg tccgaatgac tgaggcagcc
     9181 aaggaaatct gggattgttc accaatgatc tgaatgattt ctccacgtgt atggatggtt
     9241 gtttctgttg tttcttcaat cagttttata tgcttgtcgt gtgcacctaa aagcatgctg
     9301 acatcatcag cagccgtcaa tctaatctct aaagaactag actcttccgt caaaaataga
     9361 catccccttt ttttcgattc attctttcct attttaccat actcagaaga aactatatag
     9421 caaataagaa aaggttctaa aagagttgct taaccataag taccaagaaa cgttaaaaaa
     9481 tagctgcttc cattctctaa aggtctcgtt tgaataaaaa aagctgatca tgtaagctat
     9541 gcataagata gaggacaagt attccgtcta acttagcaag tattctctgg gtatttttct
     9601 tgtttcaaaa aaagaaaaca aaatatttga aatattcttg catttcaaaa aaaacaggtg
     9661 tattataacg tcaacaaata aatatacagc aaagcgtcga ggaaaacagt aggctattca
     9721 gcgatttatt cccagtgagt catgtaagtg agaaatgaca ataaatcgaa tagacgaaca
     9781 tcatttccga gtcaatgtcg aaaacgtaga tcattgtggc tgcctccatt atcaggctag
     9841 cggataactt tatcgaaaag cgtccgtgaa tgaagaagct tttttaagcg aaaaaaggtg
     9901 gtaccgttct agaatctaga gcccttttcc caagtggaaa agggcttttt tttatatttt
     9961 aaggtggtgg ttttcatgga atctgatatt agtggcattt ctagtttctg caacgtttat
    10021 tttgttactc aaaatgataa ttattggagg aaaaatttat ggaagaaaca aaaacttatt
    10081 catggaagca attattgatg gtcagttcac ttatcttcgg gatgttcttc gggtcaggta
    10141 atctgatctt tcctgtccac ttaggtcaaa tggctggatc aaactggatc agcgctggtg
    10201 taggatttgc catctcaggc gctcttttcc cattattagc tatcttagct gtagtagtga
    10261 caaaaagtga tggtctgtat gacttagcta aacctgtcgg aaaatggtat gctgccttgt
    10321 tcttgatcct tgttcattta acgatcggac cattctttgg tacaccgcgg acagcagcaa
    10381 ctgcttttga aatggctgct aaaccctttt tgcctgaaaa atatgattca ttaggtatgt
    10441 ttttattctc tgctcttttc tttttacttg cttatttcct tactgtacat ccaacacgtt
    10501 tggtcaaata tgtcggaaag tatttaaact ccttgtttct tgcattatta gctatcattt
    10561 ttgtcgtttc tttattccaa cccatgggga atctaaacca atctgctgca gattcttatc
    10621 agacgaacgc tttgacaaat ggcgttttag aaggatacaa cacggttgat gcagtagctt
    10681 tacttgcttt aagtgtcact tttgtccatg cagttcgtga tcttggtttt aaagataaaa
    10741 aagtcacaga attaacagca aaagctggta gcctgagcgt tgcgttagaa gtcatgattt
    10801 attttggtct ggtccttctc ggtgcgatga gtttgaataa gatgtcttta tcagaaaatg
    10861 gcggaactgc actttctcag atcactaatt tttatttagg taacttcggt gctagcttct
    10921 taggggtaat ggtcacatta ggtgtcttca ctaccgctat gggactcgtt gtttcttttg
    10981 cacaagattt ccataaactg ttccctaaag tcagttatat gacatggctg cggttaacga
    11041 cttttgtctc ttttgtcgtt gcgaatgccg gattggataa tatcatccaa tggtctttac
    11101 cagtattgat gcttttgtat ccattgtcac ttgcgttaat cttgctttcc ttaacagcaa
    11161 aattcttcca aaagagtcca tttgtctatc aagtaacgat gttcttttct gccgttccag
    11221 ccgtattgga tatgttggca aattcgcctg cattagtgag tcagcaaact gtcgtagcaa
    11281 gcttactagg attttatcat catcacgttc cttttgctgc attaggactt ggctggatgg
    11341 tcccaacgtt acttggttac gctggcagct tactctttta ttacgcctat cgcctatcag
    11401 ggtataaaca agaagcaaat gaactaccag aagaataacc aaatcaaaca caatttaaca
    11461 taagagatat taaacaggaa aaaaacagct agaaga
//
LOCUS       DAZUKD010000087        11276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000087 DAZUKD010000000
VERSION     DAZUKD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.089"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(321..2330)
                     /gene="metG"
                     /locus_tag="ACG8N9_002022"
     CDS             complement(321..2330)
                     /gene="metG"
                     /locus_tag="ACG8N9_002022"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4897587.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2598..2876
                     /locus_tag="ACG8N9_002023"
     CDS             2598..2876
                     /locus_tag="ACG8N9_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897588.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2973..3794
                     /locus_tag="ACG8N9_002024"
     CDS             2973..3794
                     /locus_tag="ACG8N9_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4897589.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4079..5089)
                     /locus_tag="ACG8N9_002025"
     CDS             complement(4079..5089)
                     /locus_tag="ACG8N9_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4897590.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5101..6054)
                     /locus_tag="ACG8N9_002026"
     CDS             complement(5101..6054)
                     /locus_tag="ACG8N9_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897591.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6044..7114)
                     /locus_tag="ACG8N9_002027"
     CDS             complement(6044..7114)
                     /locus_tag="ACG8N9_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897592.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7120..8163)
                     /locus_tag="ACG8N9_002028"
     CDS             complement(7120..8163)
                     /locus_tag="ACG8N9_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897593.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8164..9105)
                     /gene="opp3b"
                     /locus_tag="ACG8N9_002029"
     CDS             complement(8164..9105)
                     /gene="opp3b"
                     /locus_tag="ACG8N9_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4897594.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9189..9536
                     /locus_tag="ACG8N9_002030"
     CDS             9189..9536
                     /locus_tag="ACG8N9_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4897595.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9606..11267)
                     /locus_tag="ACG8N9_002031"
     CDS             complement(9606..11267)
                     /locus_tag="ACG8N9_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4897596.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3173 a         2299 c         2058 g         3746 t
ORIGIN      
        1 atagatgtaa taaagtcatt gcattcaaac tttatgaatg taaggacaat aaaaaagagt
       61 accagatgaa tcaattaaaa tctgcccgac cctttatcat tatgcttttt ttctgttttt
      121 acgattccta ttagtattat tctaaattaa caaaaatcag cgaagctcgt acttcttttt
      181 ctaggattta ttttggatag ttctctgttt ctgtattata tgattggctt caagtaataa
      241 gaaggaattt acgaaaattg cttctcaaat ttcggaaaaa gggggtatga cacaactagt
      301 gtcatacccc ctttgaattt ctatgcgatt tctgagccat ttggcatgga ttttggtgct
      361 tcgattactt gtaaagagcc atctggtgct tctgcagaaa ggatcattcc ttgactgatt
      421 tgtccacgca tctttcgtgg tttcagattt gctacaatga cgactttttt cccgatcaat
      481 tcgcttggat caggataaaa ttcagcgatt cctgacaaga tttgacggtc ttggctgtct
      541 ccagcatcta gtcggaattg cagcaactta tctgcccctt ctactttttg acagttgatg
      601 acttctgcta cttttagttc tactttttca aatacatcaa atttgatttg tttttctttc
      661 gttgaaacaa gttctgtttc ttctggatcc catttgactg tatcttcgtt tgtttgagta
      721 ccttcagaca ttttttcttg aataaaagca acttcttctt ccatatctaa acgtgggaag
      781 attggtgtac cttttgcaac tacttttgtt cctgaaggaa attcgccaaa gtgtaatcct
      841 tcaagattca ttgtttcaga gtccaatcct agttgattga agatcttctc tggcgtttct
      901 gtcatgattg gctgaagtaa tatcgctgcg atacgcaaag attcagctaa atggatcatg
      961 acactattca attcagcttt cttttcttcg tcttttgcta atacccatgg ttctgtttca
     1021 tcaatgtatt tgtttgcacg agaaatcagc acccagattt cagccaatgc tgtattgaat
     1081 tccatttttt ccattgcatc atggtaacgt ccgacaacgt tagcagccgt cgtagacagt
     1141 tcactatcaa atggagtgac aagtgaggca tagtcaggaa cgagtccatc acaatatttg
     1201 ttgatcatag caatcgttcg attcaataag ttccctagat catttgccag atcataattg
     1261 acacgggaaa caaaatcttc tggcgtaaag acaccatctg acccaaacgg aacagcacga
     1321 agcaaataat aacggagtgc atctaaacca taacgttcca caagcatttc aggataaacg
     1381 acatttcctt ttgatttaga catttttccg tctttcatca atagccagcc atgcccaaat
     1441 acttttttag gtagaggcaa atcaagggcc atcagcatga ttggccaata gatcgtatga
     1501 aagcgaacga tttctttacc gaccatctga acatctgcag gccagtattt ttcgaataga
     1561 ctttcatctt cggagccata tcctagtgca gtaatataat tggataatgc atcgatccat
     1621 acatacacca catgtttcgg atcactcttg actggtactc cccaagtaaa agttgttcgt
     1681 gaaactgcaa gatcctctaa tcctggtttg ataaagttgt tgatcatttc gtttttacga
     1741 gattctggct ggatgaattc gggatgttct tcatagtatt ccaatagacg gtctgcgtat
     1801 ttgctcatgc ggaagaagta tgattcttct tttaccaatt ctacttcgtg tccacttggc
     1861 gctttaccac cgatcacgtt accttcgtca tctttgtaca cttctgcaag ttgtgtttct
     1921 gtaaagaatt cttcgtcaga aacagagtac cagccttcat attcacctaa gtaaatatct
     1981 ccctgttcta ataaacgatc aaatattttt tgaacagctg ctttatggta atcatctgtc
     2041 gtgcgaatga atttatcata actgatatca agcgttttcc ataatttctt gacatcagcc
     2101 gccattttat ctacatattc ttgcggtttt acacctagtt cttctgcttt tgtttcgatt
     2161 ttttgaccat gttcatccac tccagtgaga taaaacacat caaagcccat caaccgttta
     2221 taacgagcaa ttgcgtcaca tgcaatcgta gtatacgagt tgccgatatg aagtttgccg
     2281 cttggataat agattggcgt agtgatataa aaagtttcct tttctgccac ggggctcttc
     2341 ctcctttatc gcatgatgcg aattcgtttc cttgctagta taccataaaa aaagcatgct
     2401 gttagctttt tttcaaaatg aaaaagactg gataaacgac ttctatctac gttcaatcta
     2461 tcctttaaaa ggatgaatag gttgaataaa tgcgggtttt tgttttgttt catatctact
     2521 aaaaaacggc tttttatttc tcccctcaca cgtggtataa taaagactat ctatcttagt
     2581 aagggactgg aacaatcatg cagaagaaac atcttttttt tacacttagt attgcttttc
     2641 tttcattagc acatttaata ttcagctatt tttatattcg tatgtatggt tattttaacc
     2701 tccatgggca tttgaacagt tttatgacag cagcttggat tttgcgtttt atcattgacg
     2761 tatatatcgt tatttgtggc ttctttgcca ttcgtgaaga gcggtataaa gtattgccgt
     2821 tttatctgct tttcttttta ttcaatctca tacttccatt tatttttcat atttaaatga
     2881 taacctagat ttttcaagta aatccttggt tttttttcag agtctgctac aataaggtaa
     2941 aaagtacttt tactgtcaat ggaggtggtc agatgaaccg ttttgcttct aaagaagaag
     3001 aaaaattata ttatgaacat aaagcaagtg aagaagaatt tcttaactgg tatcaaaaac
     3061 aggaacgtcc agaatatgac aagccctctt tgactgtcga tatcgtactg atgtgctata
     3121 acaaagaaga agatcagctg aaagttttgt tgatccaaag aaaagggcat ccttatcgaa
     3181 attcatgggc attgcctggt ggctttgtcc aaaaggatga gtcaactgga gaaagcgtct
     3241 taagagaaac aaaggaagaa actggtgtgg ttatttcaaa agaaaatatt gagcaactgc
     3301 atacattcag tacgcctaat cgagatccgc gaggctgggt cgtcacagtc agctacttag
     3361 cctttattgg agaagaaccg ctaatcgccg gagatgatgc caaggaagta cgctggttca
     3421 cactagaacg tcatagtaac aagatccatt tatctagcgg agaagtggct atctcgcttg
     3481 atttagtcac tggagagtct tctggcaaag atactttggc ttttgaccat agtcaaatca
     3541 tcttgaaagc attcaaccgt gtagtcaata aaatggaaca cgagccgcaa gttctacaag
     3601 ttttaggtaa agatttcacg attacggaag ctcgaaaagt atttgctaaa ttcttaggga
     3661 tcgattataa aacgatcgat cattccaatt ttaagaaagc tatgctccaa tatttcgatg
     3721 agatcggtga acgtcctgta ggaatcggcc gtccttcaaa aatctatcag ctcaagccgg
     3781 ggcaccatga ataaggttgt gagcaaaaga ttcagaccta atcattcaag aaaaaacgca
     3841 aaaacaactc tttgtgagtt acgttttttg tttcagaatt ttgatgtatt cgtaaacttt
     3901 ctgaataaaa aattaacaat agcagaaaaa gctcgtacga actttttctg ctattgtttt
     3961 attggtttgt cataattagt gagcttttct tcaaaagaaa aattcctaga aacaaaagtc
     4021 attaagactt tttattaaca aggctaaccc taacgctcct atcagtttca ttcatttttt
     4081 atcccttgat aaccgaatgt ctgcccattt tttctactac ttggctcttt cttacggaat
     4141 tgctttcttc tgctccgtcg gtattgatca aaagaaattt tttattatac cattgggact
     4201 tataacgcaa ttttgttatt aatttatgcc agtcgtgaat agaaaaatca gcatgtgcct
     4261 cttcttttgg aaaagattct acaaataagt agtcatattg tttgaccaat ttcccagata
     4321 attgttcaag ataatcttct cgctggttct tttgtttcag ataaagatcc attacttttc
     4381 tcttttgttt ctgataattg cttgcctgat ctaacctgat ctttctatgg tgagcggagc
     4441 ggactttcac attcaatttc cgctgtgcat gttgcagctt ttcttctgtc tggtgaaatt
     4501 ttggtaaagt tacttctaga ggtcgagatg tttggatcaa tgtcttcgga tgataggcga
     4561 tcccgatcca tttagctaaa gattcttttt ttactggcga aacatcacaa agtagtgcga
     4621 cataaaattc ttcattatat cgagcagaaa tcgtagcgga gcgaatcgat ccttcaactg
     4681 gccgatgctg atttatcttg attagttctt tttgttttgg taactttaag tagccatttt
     4741 ccagatagat cgtacgggtt tggttgttgg tcgtataaga ttgccacata ctttttttcg
     4801 ttttaagctt tgggaagctg gcacgtccct tgaaataatt gcgaaaagcc cgatccaaat
     4861 taagctgtgc atttgctaaa gcaagactgt ctacttcttt taagaatggg tattcttttt
     4921 ttaatgtagc gggtgtgagc gaaaaattgg ctactcctgt ttgttgccaa cttttctgcc
     4981 gctttttcag caaatggtta taggtaaatc gagcacatcc aaaagtacga atcaattgct
     5041 gtttttgttc ttctgtcgga tacatacgga atttaaaagc ttttagcatc tatgtccctc
     5101 ctagttagca accagaagag attccttttg tcgttttagt ttttttgcgt aatatgggac
     5161 ttcctgctca gttgcataga caaaatgttc tggtgcgatt tcacgaagtt gacgtacttg
     5221 tccgtcatct ggttcaggtt gatatttgat tcttctgcgt tttctctcat ggtctggatc
     5281 tggtagagga atagcagata acaaactttc tgtataagga tgaacaccgt gattatagat
     5341 ttcatcactt gttcccatct ctaataacaa gccgttatgc attacaccga ttcggtcact
     5401 aatatgttta accatcgaca aatcatgtgc aataaataga taggtcaatt gatgttcttt
     5461 ttggagatct tgcaacaaat tgaccacttg ggcttggatc gacacatcta gtgcagaaat
     5521 tggttcatca caaatgataa aacgcggttt tactgccaat gctcgtgcaa taccaatacg
     5581 ctggcgttgc ccacctgaaa attcatgagg ataacgagta ccgtggcttg gattcaatcc
     5641 aacagttctc aatagatcat cgactttttt tgcacgctct tcagatgatt tagctagtcc
     5701 attaacatca attccttcgg caatgatatc tctgactttc atacgaggat tcaacgaagc
     5761 gtatggatct tgaaagatca tctgaacatc atgccggaat ttcgtcaaac cttttttatc
     5821 tttgatcttc gttatatctt gaccgtcgaa taatatctca ccatcagtag gttcattcaa
     5881 gcgaatgatc gtacggccag tagtggattt tccgcttcca gattctccaa ctaaaccgaa
     5941 tgtttctcct tcataaatgt gaaagctgat gtcatttaca gcatgtactt cattcttttt
     6001 tcctacattg aaatactgtt tcaatccttt aacttcaagt agtttatttg gcatcagcaa
     6061 ttcctccttg accgtaagta tgtttttgtt tctctttaaa gatagcccag cgacgttgga
     6121 tttcttctgg cggtgtcact ttgggtgcct gcggagcaag tagccaagtt gctgccttgt
     6181 gtgtttttga tagttcaaaa aatggtggtt cttgttctgc atcgattttc atcgcgaatt
     6241 catttcttgg atagaatgcg tctccttttg gcgggttcaa taaatccggt ggagaaccag
     6301 gaatagcata caatcgatcg ttcgcactat ctaatgtcgg cattgaacca agcagtcccc
     6361 aagtataggg atgttgcggg ttgtagaaaa tttcttccga tgttccaact tcaacgattc
     6421 ttcctccata catgacagcg acacgatcag cgacattcgc tactacacca aggtcatgag
     6481 tgataaagat aattgatgta tcgatttttg tctgaatttc tttcagcagc tccaaaatct
     6541 gtgcttgaat agtcacatca agcgctgttg taggttcatc agcaatcagg atttccggat
     6601 aacataccag tgcaatcgcg atcacgatcc gttgacgctg acctcctgag aactgatgcg
     6661 gataactgtt gatacgtttc tcagcttcgg gtattccaac taatttaagc aattctaatg
     6721 cagctttttg tccctctttt tttgaaactt tattatgctt tctaagggac tcagcgactt
     6781 gcttcccaat gggcatcgtc ggatctaaag aggtcattgg atcttggaaa atcatcgcta
     6841 tttctttacc acggattttt tgcatctctc tttctgattt tccaacgata tcgttacctt
     6901 taaagaggat ttgaccagca tctatattgg cgttgcttga cagcaagcgc atgatgcttc
     6961 tggtcgtcac tgattttcca cttccagatt ccccaacgat cgccaatgtt tctcctttat
     7021 tcaaatgaaa attgacaccg cgaatcgctc gtacctttcc agcatacgta tcaaaagaga
     7081 tttctaagtc tttcacttct aaaattttcg tcatcgtctt cactctttca ttttaggatc
     7141 aaaggcatca cgcaatccat cagcaagtaa gttaaagcca atcatgataa ccgataaagt
     7201 tactgccgga atccataata gataaggcag atacaggaat gtcttgtacc cttcgctcaa
     7261 catcgttcct aatgaagctg acggaggcgt aagacctaaa ccgataaagc ttaaaaaggc
     7321 ttcgaagaaa atagcagatg gaatactaaa catcatttga ataataatga cactagagat
     7381 attcggcaaa atatgtttga atgcaatttt cattcttgat tcacctaatg tcaatccagc
     7441 taaaacatat tcttgatctt ttaatttcat tgtttgtgca cgaacgatac gtgccattgg
     7501 tatccagttt gtgatggcca tagcagccac gattgaaaaa attcctggtt caaaaacgac
     7561 caacatcaaa atcatgacaa cgaggtttgg aattcctgat aagatttcca aaaaacgttg
     7621 catcacgtta tcgacacgtc cgcctaacaa accagaaacc aacccgtaag ctacaccaat
     7681 cgttacatct agtaatgcag caataaatgc gatcagcaac gagattctca ttcccataaa
     7741 taagcggctt aatacatcac gtcccaaacc gtctgttcct aaaaagaaat ttacgtttga
     7801 agggacgttt gcttgagcat atttatcaac aagttcacca ccaaccattg cttttccatt
     7861 caatccatcg atattgattc ccggaatacg aggaggcaaa ttgatataag ccacgttttg
     7921 ggcagttgga tcatgaggag aaacaaagat tgtgatcaca gagatcaaaa tgatgaccaa
     7981 tagtaaaacc attgaaataa cggcagcttt gtttttcttt aaacggcgcc atgaatcttg
     8041 gataaaattc aaagaaggtg cagtaatctg ttcacgttct tcttcggtat ttttttgtaa
     8101 tggaagaaat tcatccgcag ggatatctgc aactgtttca taacgctttg gtatcatttc
     8161 catttatcct cggctccctt ctgaaacacg aattcttgga tcaactagac cgtacaatag
     8221 atccacgatc aaaataacaa aaatcagcat aaatgagtaa aggatcgtta cagccatgat
     8281 cgtaggataa tcatttgttg tgattgactt aacgaattgt tccccgatac caggaatagc
     8341 aaagatattt tctaccacca gtgatccggt catcaatgct acagctaacg gtcctaataa
     8401 agtcatcaat ggaatcaaac tatttcgaag accatgttta aaggcgattt cccaacggct
     8461 caatcctttt gcttttgcta attccacata atcactatgc agtacttcca ccatttccgt
     8521 acggataaac cgagcagagt cagcaagcgg cgacatggcc aacgcaattg ttggtaagat
     8581 tgtgtacaca aagccatccc attttgcgat tggcaaaact tgtaatttga ttgcaaagat
     8641 atattgtaat aaaacagcaa atacaaagtt tggaatggaa cgacctaata tcgctacgag
     8701 tgtagaagat gtatctgccc atgtattttg cttcatggca gagatcgttc ctaaaatcgt
     8761 ccctaggact gttccaaaga taattgcctg taaccctaac tgcaaggatg gtccgattct
     8821 tccagctaac aaatgtgcga ctggctggtt tttaaattgg aaggaaattc caaaatctcc
     8881 ttgaagcaaa ttagaaagat aaatcccata ttgtacgata actggtttat ccaaaccgac
     8941 ttgtttattt agcatctcga ttgtttcagg actcaaacgt tcctgattcg tatatggcgt
     9001 gcccggcagc aattgcatca agaaaaaggt gattgttgca attaaccaaa gcgtgatgat
     9061 catgaaaaag acacgtttta atacatattt gatataacta ttcactaatg tttcccccta
     9121 gcttttgact tctgacagat gtttcgctac attattagat aatgaaccaa aaataaagga
     9181 gcaatcaaat gaaaaaaaaa tctatcccct acgcagtagc ttttctactg attctggtta
     9241 ttttaataaa aaatctcatc aaccattcct ttacactcat tcaattatct aatgatctat
     9301 tcttatggtc attgccgttt ctgattatcg gcggtttttt atgggtcttt tcttcgggtt
     9361 tcttcgatca ttttcagcga tcggtccatc ttgcacgaac aaggaaccga aagaaaaaac
     9421 cagaattttc ctcgctctcg tctgccagct acggtatgta cagcttttgg ctgatcattg
     9481 ccggaatact aattgctctt tctgctattt tcatgctttt ttctttgtta ggataagtga
     9541 atatgagaac gaggttgcga catttgccgc agcctcgttt tttctcatgc tcacggtatt
     9601 aacgattatt cttcaacgta tgtccatttg taatcccatg aagcgcctgc cgcatggtga
     9661 acgatacctt tgattttttc attcactagg tgaccttctg ctttttggta caccggaacg
     9721 acacccatat catcagagat gatcttgatt gctttttgga aatcatccca acgggcttgt
     9781 tcatcgccag cgtctgttga tgaagctgct ttaactgctt cgtcatattc tgcattgctc
     9841 cagcgaccac ggttgtatga attacctgtg acaaacaaat cagtaaagct gcttgggtct
     9901 gcatagtcag cagcccatcc gcctagtact gcgtcaaaat cacccgaagt tgagcggtca
     9961 agacgtacag agaatggaac aggtgttggt ttgactgtta cgccatccaa gttttcttgg
    10021 attgagtttt gaacatattc gattactttt ttcgatgaat catcatcaga agccatcaaa
    10081 tcaaattcta aagaatcaac gcccaattct tttttcgctt tttcccaata ttcttttgct
    10141 ttatctttgt cataagaaac taattttcca gcttcttttg caaagtcttc gttcgttgtt
    10201 ggacttttta ccatatctga tgggatcaat cctgtagaga caactgatcc gtcacctagt
    10261 acttgtttta ctaaagcttc acgatcgata gaataagaaa tagcttttct taggttcaca
    10321 ttgttgaatg gtgaattctt ttcacgttga ttgaattcga tatagcttgt ccgcgcttct
    10381 ttaacagaag tataagctgg atcgtttgcg ttttgctgtg ctaactcacc agacaagatc
    10441 acgtcatctg cttggccatc tttgaataag ttcaatgcag tagaagattc ttttactaca
    10501 ctgactttga tttcagaaag ttttactgta tctttgtccc agtaatgatc atttttctta
    10561 taagtccatt ctgtatctgt tcctggtcca tcaaaatctt ctaatgtgaa tggtccatta
    10621 taaacagctt tatcacttgc agatgcatag gcatccccat tttcttccac tacggattgt
    10681 ttttgtggga aaaatgatgg gaaagcaagc agatattgga aatatggtgt ttgtttcgtc
    10741 aatttgattt ctaattcata gtcaccaact gctttgatcc ctagttctga tttatctttc
    10801 tttccagctg taatgtcttc tgcattttca actggcgcat acaggtaagc atattcagaa
    10861 gctgtagctg ggtctactgt tcgttgccag ccataaacgt aatccgctgc tgtaacagga
    10921 tcaccatttg accattttgc gtcttctcgt aatttgattt tgtaagttaa tccgtcttca
    10981 ctgacttctg ccaactcgct agctcctgcc ggctgtggtt tgctgtcttc atcaagacgg
    11041 taaattcctt cataaacgtt attcaatgct gtaaaactga ttgtatctgt tgcaactgat
    11101 aagtcagctg taggcatttc ttgaggcacg actaagttaa atacgcctcc tccatctgct
    11161 gaaccgcttt ttgctgaact actgcttgtg gctgaactgc cgccataaca tccagcaagt
    11221 acaaggccgc ataccgcaac caaaccaaat gtaaatttct ttttcatttt acgtac
//
LOCUS       DAZUKD010000088        11259 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000088 DAZUKD010000000
VERSION     DAZUKD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.090"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..2099)
                     /locus_tag="ACG8N9_002032"
     CDS             complement(27..2099)
                     /locus_tag="ACG8N9_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4897597.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(2112..2387)
                     /locus_tag="ACG8N9_002033"
     CDS             complement(2112..2387)
                     /locus_tag="ACG8N9_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897598.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2749..3204)
                     /locus_tag="ACG8N9_002034"
     CDS             complement(2749..3204)
                     /locus_tag="ACG8N9_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897599.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3358..4566)
                     /locus_tag="ACG8N9_002035"
     CDS             complement(3358..4566)
                     /locus_tag="ACG8N9_002035"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4897600.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4678..5010)
                     /locus_tag="ACG8N9_002036"
     CDS             complement(4678..5010)
                     /locus_tag="ACG8N9_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4897601.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5158..6033
                     /locus_tag="ACG8N9_002037"
     CDS             5158..6033
                     /locus_tag="ACG8N9_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4897602.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(6115..6696)
                     /locus_tag="ACG8N9_002038"
     CDS             complement(6115..6696)
                     /locus_tag="ACG8N9_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4897603.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6702..7961)
                     /locus_tag="ACG8N9_002039"
     CDS             complement(6702..7961)
                     /locus_tag="ACG8N9_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4897604.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(7963..9009)
                     /locus_tag="ACG8N9_002040"
     CDS             complement(7963..9009)
                     /locus_tag="ACG8N9_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897605.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9244..9519)
                     /locus_tag="ACG8N9_002041"
     CDS             complement(9244..9519)
                     /locus_tag="ACG8N9_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4897606.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9762..11072)
                     /gene="der"
                     /locus_tag="ACG8N9_002042"
     CDS             complement(9762..11072)
                     /gene="der"
                     /locus_tag="ACG8N9_002042"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4897607.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         3279 a         2367 c         1873 g         3740 t
ORIGIN      
        1 aacttgaaaa tttttacctt tttattttat tggactcgtt tttttgactt tgctcgaaac
       61 gcaaatcgga aagaaaagtt atcagatggt ttgttcatcg tataagattc taacaaattg
      121 atattcctgc gatagctgtt cgcagaaaac gtcgtgattt tctcagcaac ttcgtattca
      181 tcaatgattt ttctttcaag ataataacct cttagcattt tttttgtaaa cagactatct
      241 tgctggatca tccacgaaga aatcaaattc cccgagttca tttttattcc ttcaagtttg
      301 cgttgctgga taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa gttatctaaa
      361 ctttcaataa tcaactgagt atttttttga tatgtctctc tgatttcttg gatatcttca
      421 tcactaataa atgtctgacg atcagcatgt cttctttgcc aaaacatttt cggatggaga
      481 acgattcgca ttaacctacg gacaacaatg aaccaaaagc cgataaaaga caaaatctgg
      541 cttgtgatag aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata accattagaa
      601 gaaagcttgc ccgaacgata actttcatcc aatccatccc gttcgatcga caaaatcagt
      661 gcttgtattt cttgtacttc ttgcttttca tcatcggtta aatcttccaa gtacagatca
      721 cgtatccgtt cttgataatt ttctatcact gcttcagtgg ctaaaaattc ttttgtatta
      781 cgatcaattt cctttatttc ttttcgcaaa acttcaacga cctcttctaa aatcatcaaa
      841 gcattcaaat ccgtgctttt cgcttcctct gtttccgtca gcataggcaa gacaatgatc
      901 cctatgacca aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa agaccgctgg
      961 taaaacatca tattattgat tgaaaatggc aaaataaaaa tcgtagccaa actgactgtt
     1021 ccctttacac cgccaaatgt taagatcaag atctcgttca actgtttttt gaacttttta
     1081 ctgccatctt tgaatacata aaaaagactg atagaaatga atctgatcac aaaaagcatg
     1141 acagagatta atacgataat cgctaataaa agaccgtttg gatataaacc gtctccccat
     1201 actggcgaga acacttgcgt caattcgatt cctaaaaata taaatactaa tgcattcagt
     1261 gtaaaagcaa tagttgtcca tgtactgtgg gacaagctcg acaattctgc atcaaacaca
     1321 gagattttcc taaaaccaga agcttggaga atacctgctg ccactgcagc aatgattcca
     1381 gatacatcaa atgcctctgc caatacatag gctagaaaag gaagcaaaag ttcgatcagc
     1441 agataccctg tgacatcttg tgctgatgct ttttcgatca aatgaattat tttttgtttg
     1501 aaccagacaa gcaaaaagcc gatcaaagct cccccaatac tagaaactac tagactcatc
     1561 cccgcgttta ctgctgaaaa agatccggtg atcagcgcac caagagcaaa ctggaaggca
     1621 gtaacaccag atgcatcatt caaaagacct tctccttcca aaatatgcat cgccttttta
     1681 gggatattca ctcgtccaga aagagaactg actgctaccg catctgtcgg acctaacgct
     1741 gctccaaaag caaagcaggc agcaagtggg acacttggca acaggaatga taacgtcgca
     1801 cctaccccga caagggtcaa aatgactcct ccaaatgcca agaacaaaat tgtcccgaaa
     1861 tttttgagaa tcgaagaaat atctgctttt tctccttctc taaataacaa tggtgcaata
     1921 atcataacta agaacaattc aggttcaaaa tcgattgatt gtccccactc tgtcagacct
     1981 aataccactc caaggaaaat ttggatcagt ggagctggaa tcattggcaa aatgcggcta
     2041 gctatgttcg ataaggtgat cgttactgca aatacaataa ctaactcagc aaattccatc
     2101 gtccatcccc tttatttttt attttgtgta agcattttta aaaattgttc ttttttcttt
     2161 aaaattttgg tttcgactac ttcaacctgt gtttggttac cctctaattc acactgtaca
     2221 cgttttgact ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg atacatcaat
     2281 atatttcttt tacgcaactt acaaagtatt tgacggcggt catccatcga aagggcgcca
     2341 tagcgatcat gcaacagttt tttttgttgt tcaaaactct ctctcatttt ccagatctcc
     2401 tatacacaaa cttccatcat tatagcaaag ttctttttgg atgaaaaata tttcattgtt
     2461 tcatcgttta ttgctagtta gaaatttcca taaatttttc aacgcctcat tctattccaa
     2521 atagtatcag atatcaagta aaacttaaga aagataaaag tttctgacaa aagaatgttt
     2581 acttttccat tcctgccttt cttgttcttt tatcacaaca aaaaaatggg gctgcgacaa
     2641 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
     2701 taataagaca catctctttc acagcttcat ttttgcgtaa aaatcgattt attcgaaaaa
     2761 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
     2821 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
     2881 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
     2941 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
     3001 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
     3061 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
     3121 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
     3181 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
     3241 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
     3301 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
     3361 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
     3421 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
     3481 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
     3541 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
     3601 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     3661 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     3721 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gacccagttg
     3781 gctgatcaat aatgtccata cttggctctc ttctttcaat gggacggatg gtaattcagc
     3841 aaaacgtaaa agtgcttcgc cttcttcttt caaactggga cagcaaatgt agcactctgt
     3901 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     3961 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     4021 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     4081 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     4141 ttctaaatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     4201 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     4261 atgatctggt ctgcggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     4321 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     4381 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     4441 tggctggttc agcaagatat cacgtacact tccacctaca aaataagctt catatcctgc
     4501 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     4561 tttcattttt ccaacctttc ctgcgtgttt cttgtactga gataaagact cagttgcatg
     4621 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca gaaccacttc atcttgttta
     4681 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     4741 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     4801 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     4861 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     4921 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     4981 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     5041 ctttaacgca aatcctagat aagagcagtt gagaaaatcg aatttccatg ctacatttag
     5101 aaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     5161 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     5221 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggcgt ctcagggatc
     5281 accttgatct taagagcttt attcggaatt gatccagctt atacgacttt attaatcaac
     5341 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatacagtt
     5401 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     5461 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     5521 agcgggatcg tctaccgcat gggtggaaca acaggtggca gcgatattat tgctcggatt
     5581 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     5641 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     5701 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     5761 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     5821 ggtgtgactt ttctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     5881 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     5941 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     6001 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     6061 aaagattcca catccagctg atctttgtct tttttagtac tatactattt ttaactaatt
     6121 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     6181 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     6241 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     6301 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     6361 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     6421 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     6481 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     6541 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     6601 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     6661 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     6721 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     6781 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     6841 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     6901 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     6961 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagattg
     7021 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     7081 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     7141 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     7201 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     7261 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     7321 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     7381 ggtgcgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     7441 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     7501 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     7561 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     7621 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     7681 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     7741 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     7801 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     7861 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     7921 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     7981 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggacgttg
     8041 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     8101 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     8161 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     8221 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     8281 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     8341 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     8401 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     8461 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cttgccgctc
     8521 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gtactacacg
     8581 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     8641 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     8701 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     8761 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     8821 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     8881 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     8941 ctggctgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     9001 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     9061 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     9121 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     9181 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     9241 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     9301 tggaatttcg atttcttcac cagtttgtgg gttgcgacct ttgcgagctg cgcgttcgcg
     9361 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     9421 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     9481 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     9541 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     9601 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     9661 aaggttttta ccaattttca catacttttt ttagtcatag aaacgtaaca aacgaaactg
     9721 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     9781 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     9841 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     9901 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     9961 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
    10021 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
    10081 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
    10141 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
    10201 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
    10261 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
    10321 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
    10381 gctgtatcga tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
    10441 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
    10501 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
    10561 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
    10621 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
    10681 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
    10741 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
    10801 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
    10861 tgatccataa agggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
    10921 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
    10981 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
    11041 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
    11101 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
    11161 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
    11221 ctgctttcat ctcagtcaca gatacaaaat caattattt
//
LOCUS       DAZUKD010000089        11137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000089 DAZUKD010000000
VERSION     DAZUKD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.091"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8N9_002043"
     CDS             6..305
                     /locus_tag="ACG8N9_002043"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4897608.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8N9_002044"
     CDS             378..1739
                     /locus_tag="ACG8N9_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4897609.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8N9_002045"
     CDS             1761..2021
                     /locus_tag="ACG8N9_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897610.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8N9_002046"
     CDS             2035..2787
                     /locus_tag="ACG8N9_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4897611.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8N9_002047"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8N9_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4897612.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="ACG8N9_002048"
     CDS             complement(3542..4516)
                     /locus_tag="ACG8N9_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4897613.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIK
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="ACG8N9_002049"
     CDS             complement(4894..5613)
                     /locus_tag="ACG8N9_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4897614.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG8N9_002050"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG8N9_002050"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4897615.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="ACG8N9_002051"
     CDS             7164..8561
                     /locus_tag="ACG8N9_002051"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4897616.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="ACG8N9_002052"
     CDS             8642..9082
                     /locus_tag="ACG8N9_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4897617.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9228..9554
                     /locus_tag="ACG8N9_002053"
     CDS             9228..9554
                     /locus_tag="ACG8N9_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4897618.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9555..10304
                     /locus_tag="ACG8N9_002054"
     CDS             9555..10304
                     /locus_tag="ACG8N9_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4897619.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10330..10740)
                     /locus_tag="ACG8N9_002055"
     CDS             complement(10330..10740)
                     /locus_tag="ACG8N9_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4897620.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3533 a         1947 c         2291 g         3366 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagttttgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tctttgcttt ctgttccttt aattttttgt aaaaaattac tttacctgat gaagtaatca
     9181 tgctattcta attacaacga cagatggagt aatatggagg aaagaaaatg atttcaagtg
     9241 atggaatacg cggatacaat gatgcgatta ttttatcgat ccttaacaaa aacgattctt
     9301 atggatatga aatttctaaa acgattcgtg agaggacaaa tgattcctat gtgatcaaag
     9361 agacaacact ttactccgtt tttgcccgtt tagaaaagaa tcaactgatt gtttcgtatc
     9421 ctggagaagt gactcatgga aaaaagcgga catattataa aatcactgaa atagggcgta
     9481 atcttttaga agaaaaaaag caagagtggc tgacgacgaa agacgtagtc gatcaatttt
     9541 tggagggaaa ttaaatggat acaatccgta attatttaga cagtcttttt atcggagtac
     9601 ctcaaagtac cgagatagat aagctgaaaa ctgatttgtt ggcaaatatg gaagaccatt
     9661 atcatgaatt gatgggagaa ggaaaaaatg aacaggaggc catcggtaca gtaatttcga
     9721 catttggttc aatcgatgaa ttattagaag aactagatgt cgaaaaaaaa catcaagctg
     9781 acgagacgga aacaaacaca gcttctattt atttaagcga agcagaaaac tactggaaag
     9841 aatacagagc ggcaagtctc caagtggcat caggtgttct attcattagc ttatcttttg
     9901 caagttttct tttcttttgt tcagcagggt atgtgttcat gggtatttct tgtctgattt
     9961 tcggtatagc attagctgtc ggttttttta ttgcttctgg tatgaaaatc acacgtttaa
    10021 atcacttttt gcaccatcgg aaaatacctg aaaaagtatt agcagaagca aaagaaaaag
    10081 aagaagagta tcagcgaagt tttggcttct cactgattgc aggaattgga ttatgtattt
    10141 tttcactttt cccacttttg gcttctttaa tgtggtatat ggatggtagt atcggtgcct
    10201 ctatattttt tgttacggta ggaacgggag tcttcttgat catttatggc agtctggtcc
    10261 gtcattctta tcgtcaattc actcaaagtg cttattactg gtgaatgtat tcagatttgt
    10321 aaaaatattt tatttattgc ttacccatgt aaaatcgtta tagacagatt tcagttgact
    10381 tctgcgccat tgtacaggga taaaaagacg catactatct tcattgaatc cgactaataa
    10441 tttctttccg tcagtaatga tgggacgttg taacaaagta aaatgcgaga cgatcagatc
    10501 gacaagctct gaggtcgtac attggtcgat ttgatctttc aaatttttat aatcaatcga
    10561 gcgtttagct aatagatcgt caaatccgtt ttctgttaat gacaataaat aaaatatctc
    10621 tttccttgtt aaaggctgtt taggtgtgaa aattcgttct ctgtaaggca tttgatggtc
    10681 tttcagccag tttgccatca gatggtaact atgtgtgcga gaagaataga tcgtaagcat
    10741 aaaaaaactc cttaaatagt ccattaattt gttagatctg ttgagataaa taataggtaa
    10801 ggagctgtgc ataagcattg atcaaaacaa cccaaacatc attagaaata actttctaaa
    10861 gtcttaaact tgcgacgaga aacaactaga ctaatagaaa aaatgtctta gccatttggc
    10921 ttcctgtttg tttttttgct tatgctgcag ctcctcggga aagggttcta acaatttgaa
    10981 gaaggttgat gattgatcta gtttaaattg tgtgaaattt gaaatctggt gtttttacta
    11041 gatgaatcac ctaacaacta tgattataac ttatagaact tctgttttgt acttggtttt
    11101 gttatagttg ttataaagtt ttattttatt acagaac
//
LOCUS       DAZUKD010000090        11056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000090 DAZUKD010000000
VERSION     DAZUKD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.092"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..247)
                     /locus_tag="ACG8N9_002056"
     CDS             complement(<1..247)
                     /locus_tag="ACG8N9_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4897621.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIK"
     gene            complement(240..1832)
                     /locus_tag="ACG8N9_002057"
     CDS             complement(240..1832)
                     /locus_tag="ACG8N9_002057"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4897622.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2342..3067)
                     /locus_tag="ACG8N9_002058"
     CDS             complement(2342..3067)
                     /locus_tag="ACG8N9_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897623.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3221..3547)
                     /locus_tag="ACG8N9_002059"
     CDS             complement(3221..3547)
                     /locus_tag="ACG8N9_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897624.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3584..3949)
                     /locus_tag="ACG8N9_002060"
     CDS             complement(3584..3949)
                     /locus_tag="ACG8N9_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4897625.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3986..4201)
                     /locus_tag="ACG8N9_002061"
     CDS             complement(3986..4201)
                     /locus_tag="ACG8N9_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4897626.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4330..4665
                     /locus_tag="ACG8N9_002062"
     CDS             4330..4665
                     /locus_tag="ACG8N9_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4897627.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4741..6738)
                     /locus_tag="ACG8N9_002063"
     CDS             complement(4741..6738)
                     /locus_tag="ACG8N9_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897628.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6836..7111)
                     /locus_tag="ACG8N9_002064"
     CDS             complement(6836..7111)
                     /locus_tag="ACG8N9_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897629.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7343..8077
                     /locus_tag="ACG8N9_002065"
     CDS             7343..8077
                     /locus_tag="ACG8N9_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4897630.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8116..9258
                     /locus_tag="ACG8N9_002066"
     CDS             8116..9258
                     /locus_tag="ACG8N9_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4897631.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(9886..10371)
                     /locus_tag="ACG8N9_002067"
     CDS             complement(9886..10371)
                     /locus_tag="ACG8N9_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFS4897632.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(10373..>11056)
                     /locus_tag="ACG8N9_002068"
     CDS             complement(10373..>11056)
                     /locus_tag="ACG8N9_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897633.1"
                     /translation="QINDLSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAV
                     DYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQ
                     DVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQ
                     NENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIV
                     SLINSIQED"
BASE COUNT         3614 a         2082 c         1563 g         3797 t
ORIGIN      
        1 atttgattaa ttcattatat tttttttcag taattttata tcttgtttcg taattatcat
       61 aaatagcatt ttgttgttca tagacaacaa aattgctatt agatacaaag aattctttct
      121 ttaaggcact cccaaatggt cccctcctta ctccatcttt tttatcaagt aagcattcaa
      181 acttacgctg ttcccaagct tccgtaaatc ctgggaaacg aatttctggt tgtatatcat
      241 tactcatgtt tgaacacctc cagcgcacct tgtaaccacg cttcattttc aggtgttgtt
      301 tgcagggaag agatagcttc taaaagttct ttttctagca cttgctgttc ttgtcgtatc
      361 ttcttgatgt catttccgac atgaaccata tcaattggtt cttcttcttc aaacgtatct
      421 acatagcgtg gaatatttaa gttatagtca ttttctttga tttcatcaaa gtttgccaaa
      481 tgagcgtatt tctcgacatc ttttctttca gcataggttt cgaggatttt ttgaatgttt
      541 tcttccgata atttattctg attttttcct ttgacaaatt ctcgactagc gtcaatgaat
      601 aatacgtctc gagtttggcg gttctttttc aatacaatca ccgtcgttgg aatcgatgtc
      661 ccgaagaaca agttcgcagg cataccgatc acagcataaa tactgccgtc ttctagtaat
      721 ttttgacgaa tgaccccttc tgcagcgcca cggaataaaa ctccgtgtgg caagacgatg
      781 gccatagtcc ctgtttcctt caaatgatag aaaccatgaa gaagaaacgc aaaatctgct
      841 ttggattttg gcgctaattt tccataacga ttaaaccgag aatcgtctaa gaaggtcgta
      901 tctgctgacc aattagccga ataaggtggg ttcattacta ctgcatcaaa tgtgtaaggt
      961 tcatctgttg gccaatcctt attcaacgta tccccattgc ggagattcat ttcttcggca
     1021 tctaccccat gtaaaatcaa gttcattttt gctagattgt atgtcgttgt atttaattct
     1081 tgcccatgat atttaacgtt atctgggtga gttaagtagt tacgcacatt taacatcaat
     1141 gaacctgaac ccatggttgg atcaaaaaca ctaaagaatc gtcgttcttt ttgatccaac
     1201 gtcacgattt gtgccatcat atcagaaacc atatgaggcg tgtaaaattc tccggctttc
     1261 ttccctgctt ctgaagcaaa ttgactgatc agaaattcat aagcatcccc gatcacgtcc
     1321 ccatcgtgac ctaaaacttc cacgtcattc aatttcttaa tgacttcggt aatcgtcaca
     1381 tttcgttgtt gctcatctgt tcctagtttt tttgattgga gatccacatc atcaaataac
     1441 ccattaaatt gattgtaggt agatgccaat tgcacaaagg ctttgtttag atcattcaat
     1501 tggaacgttg cttgttttgc ttgatccgct aagacgttga aaagatactc tggcgaaatc
     1561 gcatacccca aaatatcaac gattgttgca atcagatctt cttttgattc ttcatctgaa
     1621 agtaattcgc gatacaacat cgtttgtttg cttaccgtat catattcctc taaggattca
     1681 tctgctaaaa gaaccacttg ttccaacaaa cggtcggata aatatttata gaaaatcagt
     1741 cccaataaat aatttttata ttctgacgca tccattttgc tacggagatt atctgccgca
     1801 ctaaataatt tttgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa
     1861 gttgctatat accctcttat tttagcatac tcccttttag aaaaagcaaa aaaaaaaaga
     1921 aagaaactta ttcttgctaa taaactacgc gaaaggagtc taacgttgtt tgatataact
     1981 tagactgatc gttaacttat tttttatatt tctacatagt tatctttaga accatttcag
     2041 atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat
     2101 aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa
     2161 cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat
     2221 agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat
     2281 gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc
     2341 attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata
     2401 ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatatct ttaccaactt
     2461 tctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag
     2521 catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagtt
     2581 tccaagtagg tggtatagct gtgtatgttt ctacaaatga attaagtttt tcattaagtg
     2641 acttctcttt tatatttcca tctaaaaact ctgtatatat ggcttgagct atactaaaac
     2701 aaagctggcc ttgttctaat aattttacat cattagttag taaagctgtg ttcacgttat
     2761 caatcgcttc aagaagagta tctttggtgt ccctaacagc tttaggtgtg ccttctggaa
     2821 tttccctact tgatacctcg tccattttat cttccataaa ccccttaaaa tcatatcttt
     2881 ctcccaaatc catagaagtt tttgagaaag ctttcatttc tgagtctatt tcttcaataa
     2941 tatttaacat gtctgataca tattgtttag gctgattcgt ttttaatcta tatgtggtaa
     3001 atatctcaat tcctccaatt atcaataaaa ttgccaaaca aatccataat aattttttct
     3061 ttttcatttt tcctcctata cactgctgtt ataacagtga ctaatgtaaa gattttccca
     3121 gcaatcatca gactattatt tacagggaat caaaaaaact ccttagtaag cttacacctt
     3181 taggagttcc tgtttgaacg gtatgcataa tacatttaaa tcaatttaac attaacacct
     3241 cagtatatgg tatctctaca tatacacggt ctttttttgg atagtactcg acatttatat
     3301 tgatagcact tccattgtct aacgtagctt tagcccggta cgtgttatca ccattatcat
     3361 gagtgatcgt cacatcatca catgtccgat ttaagttata ctgttcttgt aaaatctgag
     3421 ttaccaatgg tacagatgac ttctctagtc cagatgaacc cgtcattctt gaaaaaatta
     3481 tacctataat agccaataca acgataccta taatgacaaa cttcttattc atatttactt
     3541 tattcaactt tttatctcct atctccaaaa tgattttctc tcatcagtaa ctcattgaca
     3601 tccccaatag tacaccaaag atggctccag ctacccctgc tatcatcaaa atcgtaccca
     3661 tttgttcatc tttctctcga tataagtaac ccataataac agcaacagca ccaaatacca
     3721 caggaataaa tgctaatgat actaccatag aaatccaacc aataatcaaa tacagcttcc
     3781 ctgtctcttg tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt
     3841 tctttactgt gtctgttccg gttgtctcat ctgtttcttg taatgctccg caaaactggc
     3901 aatacttact ttcatttgtt atttctttac cacattttct acaatacatg tcttctcctc
     3961 ctaaaatcat tcaattgctt attagctaac tgtttacttt attaaataca tcagaaaaca
     4021 gctcatttat tggtacactt aatatctcag aaagcatttt catttgatag ggtttaggaa
     4081 tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt tttttggcta
     4141 actcagtttg ggaaagtcct ttttcttttc tgtatcggct gagattactt acttttttca
     4201 tgattctccc cttctgtttt attatataca acaatatgat gtatttcaac tcataaaata
     4261 tataaatact tcaaatgtca ttacaaatac aacaacttgt tgtactctac tgagaagggg
     4321 ggtgtattaa tgaaacagaa gcaatatgca aaggtcatta gagaaaagcg aaaagcactc
     4381 gggtggacac aaaaagaatt agctaaaaaa atcttctcta cacaacaggc agtagcaaga
     4441 tgggaaaatt ctgtaacaga acctaacctc gatagcttaa cagctctttc tagagcccta
     4501 ggaacccctg taagccattt tctagacaat gttgacatag attatgagga agagtttttg
     4561 gctctctatc gctctcttag tactgaagat gcaattcgaa caattgacta tatgaagcta
     4621 ttaaaaaggc aagaaaatga acgcaatcag ctattgaaag attgaaaaat aaataaccca
     4681 ccgaacttta acggtgggtg tattgagatg aaataagatt aagagctatg gataacctgt
     4741 ctagtttaat ataattgggt ctcttctatt ccttactctc cgtccatcat ctcttgttac
     4801 acttgtaaat tgataaatag gaactaattc aactcctgat aatatcaatt cttttattac
     4861 ttcatctatc cctgcccctt tcgctagata tactaaaaaa ctactgcttg ctttcacatt
     4921 gtatagtttc ttaatatcct tagaagctcg ttcaagagtg aatccttgtt taacaaagta
     4981 gcctctgttt tctattaaag catttttcac gctgttatcc ctgacatttc gtaggcgttc
     5041 acaaacagtg ttaataagtt ctttttctgt cattagatat gtcccttgcc acgataagcc
     5101 ctgtaaaact tcatgtgctt catctaaaat caattgtttt tccttgctaa gttctgatcg
     5161 ggaattttga ggaaatactc ttttagcctc tttttcgtta aaacatatct taacccattc
     5221 ataagaaaaa tattgatatg taaaatgaaa tttttccaga agtggaagga tatcttcatt
     5281 caatttttga atcacagaag ttgcattaaa aacgtctaat cgatagacat tgcttctctt
     5341 atgtttaaag tttcctaacg ctttctcttc ctttaaccta tttaggtatt cgagcatctt
     5401 caaaggaaca tcttcatcat ctaatttttt tatcaatcct ataaaagtca taagtgatag
     5461 taacttatta catttatcta tgtcataagt tttaccatag taagaactca tttttttagc
     5521 taattgttcc cccgacaaaa tacaagtcac actttcctta ctatcatcaa aaaaattaac
     5581 atcagaaagc aagacatcat agacacgtcg catttcctct agataattcc ttattgtcct
     5641 gaaaagtttg tctttcattg attcaagagt gtcaaaaagt acttttgatt cctctttcaa
     5701 ttttcgaaaa tgtgtaggat acaagtctag gttctctaat agccaattaa atgtttctac
     5761 ccgactgtct ttctttaaaa ttagcatcac aactccaata aaattttcac tgatatcaca
     5821 attactggaa tgacaataaa aaatggaatg cccagtttta ctggtaaaaa tactagcaga
     5881 gggattatga tcgttgtgaa acagacatct taagttttta cttgacttaa gtaatttatt
     5941 catatcaatg gttaacaatc tttcataaat ctcattaaaa gacatgtcgg aggaatcaaa
     6001 caggcagtct ttaaaccact gtttcatttc ttctttgtcg tcatttttta tcaacttaac
     6061 aaaatcggtt tcattgacta gttcatactc tctccctgaa tcactttgac aatttttaga
     6121 acatgtcatg gtaaaatcaa tcggaagctc agtaatatct aacatgttgt cttcattaat
     6181 tattatcact gcatctgact tatatcctcc aaaaaacatt ctattcgaag catttacatt
     6241 gtcatcacac ccggggatgt tctttttcaa gtattcgtaa acagcaacca ttttaccata
     6301 gtcagtaatt gcttctttta atagaaagac aatcttgtat ttttctagat tttcagagta
     6361 ccgaatactt ttttgtatcg caaacgcaga attatgaact aaattgatag ataaaacctt
     6421 ttctatgctt aagtatctac tatcatcttt actcaaatct actaccttcc ccttctcatc
     6481 tttaacagtg ttatcgacat ccagaataat tactctggtt tccacaaaac tctccttgtc
     6541 attgccttta ttctttgtct tgcttggaat aaatgattgt gatgaattta gtatctctac
     6601 aaactcatct cttgtaactt ctctatagtt atctaaacac ctatctctta catcagtttt
     6661 ttcttttttt gtaactggct tctctaataa tttttcttta tctacataaa ttggaatatt
     6721 aatcattgat gtttccattt gtgtttcctc cttataaaat tctgttaaag actaaaacat
     6781 acagataata aaaacaaact ctttattact atatgtttta gtcttatttg tttactcaac
     6841 ctttataccg ttccatgaaa ctatcaatat cagatttttt aaatctaatg accgtatcaa
     6901 tttttataac aggtaacccg tattgagaaa catattttct aatcgttcct actgatacac
     6961 ctagatattc acttgcctgc tgatagtcca tatactctag agtgactcca gaaataatgt
     7021 cttctagcat aggctttagc actgtaatta tctctttcac ttgttgttca acttgtatag
     7081 aaacaatgct atcaatgatt tgtagagaca tatttccccc tcctttcgtg attgcttgaa
     7141 caaattttct aagaattatc ttcgtatttt ttgctcaaaa taaaagtatt ttgttattaa
     7201 ccgattaatc aaacaaaaaa tgattaccag ttcatcgaca tacacataat acctcgataa
     7261 aaaaggggtg tcaatagttt tgtaaaattt atttacaaca cccctttttt gaggtatact
     7321 aattttagga ggtgtggtca atatgataaa acgtccacca ataaattatt tagaacgaaa
     7381 aaagatactc ggaacaaaaa taaaagctat aagaaagagt aaaaaattaa cacaaccagc
     7441 atttggtctt atgattaaca acggtcaatt aattgacaaa aagactatct acgaatggga
     7501 gaagggaacc tatttaccaa taccagagcg attatcccga attgctgatt tgggaaatat
     7561 gagtatagag gagttagttt gtggcaatgt agaagaatat attttgggaa ttattcttta
     7621 tagagatagc attgtactag atggtataac atttccagat aaaaacctgt tccagcatct
     7681 tcgtcaacag ttcccaccag tacactctaa tttagataca tggctggata gatattccaa
     7741 attagaacca gagatgcaag aatttattgc taacaagact tgtaataaag ttaaaaatga
     7801 aaaaattagt cttttcaata tcttaaaaat agaagaatta ttcatcaatg ctattgtaga
     7861 agaatttgac aataacattc ttttcctcac tagcagtata gaagaactac tagaaagaat
     7921 ggtagatgaa tggctaccta ttcaattgaa agacatgtct tatcccgaag aagctgtgag
     7981 agaaattact gataatataa acaagctaga acaaacgatt tcatcaattg ggaaaaaata
     8041 cactaagaaa aaaatgaagg gaggtgatac catctagtgg taagtactcc ttgtaaacta
     8101 agaggagatg atttcatggc aaaaatatca aagtacaaaa agaaaaacgg tcaagtggcg
     8161 tggatgtata aaggacacat tgccaccgat ccacgaactg gtgaaaaaat aaatactacg
     8221 agaagaggtt ttagtagcaa acaagaagct caaaaagatt atgacgagta cagacatcaa
     8281 atattatacg gtgttaaaaa gaaagcacct gatatgacat ttgaagacct gtataatgaa
     8341 tggataactc atcagcgtac cagtgttaaa gcttcaacaa tcgcaatctc agtaagatac
     8401 gcaaataatc aaatacttcc agcatttggt aagcttaaac tatcgaatat ctctgtccca
     8461 tattgtcaaa aagtggtaga tgaatggcac agtaaatatg aaagttacga ttatatgaga
     8521 aaacagactg cacaaatttt acgctacggg gttgcaatgc aatatattga caataatcct
     8581 atggaaaaaa cacttcttcc acgaaaaaaa gagtatgaga aaaatcgtaa attttactca
     8641 aaagaggagc ttaataattt actggatgct tttaaagatt ttggtaacat gaaacaatac
     8701 gccttcttca gattattggc atacacaggt atgagaaaga gtgaagtact cgctttacag
     8761 tggaaagaca tagacacgtt taataaagaa ctacatgtca ataaaactct tgctgtagat
     8821 gaatttggta aagttataat tcaatctccg aaaacaagag cttctagacg agttatctct
     8881 ttagatactg agactctttc tatcctcaac aactggaaat tacagcaaaa agaagagtat
     8941 ttaaaacttg gatacaatac tagctctaaa gagcaacatg tttttacaac agttaaaaac
     9001 actctataca ttccaaacac tgttaatgat tggttaagat atattttgaa aaaatataac
     9061 ttgcctcgta ttacacctca tgggtttaga catacacatg ctagtttatt attagaagca
     9121 ggagaatctg tcaaggtagt tcaacaacga ttagggcatg aaaatagtaa agtgacatta
     9181 gacatctatg cacatattac aaataatgca cctaagaaaa caggtcaaga ctttgctgat
     9241 atgatggctc atcagtaaaa gagctggtca aaatctggtc atttctagta tacaggcaaa
     9301 acaaaaaagc tagaaccctt atatatcaag ggttctagct cctataacag gtttacccaa
     9361 cactgccttc caatgactct tttgggaatc agaggaaaga atcaggcttt atagagatat
     9421 ttcatctaac aaattacttt agactgacag tatatatcag caggtactgt catcgggaac
     9481 gtagccaata tcgtatccaa aacagcaggt agagaagcta tttgaggatt caagttattc
     9541 tatttcaacc atgaagttaa gttcattaaa aataaagata gttggtattt caataatttt
     9601 tgtatagagt taatcacttt attgatgcta attcatcttg attggtatta taatcgtatt
     9661 caagttgcgt ggcgtgcttt tgctagaaaa tatataactg ttctttataa agaaatataa
     9721 ggattaaaca ttttttatca tagagtttta cttatctata gaattatgtc tgttcctaat
     9781 tcagtttgat gaattaactg ataaaaaaat atctactatt taaagataca gggataagta
     9841 agtaaaaaaa taacatgtca ttagatagta gacagttcca actctttatt tacctttttt
     9901 gtatgctgca ctgtcaatcc aagttcctaa attgttgtat ccatcatcac ttatataatc
     9961 atattggcca tcaataattt cgtcaaacca caaatattct ccatctggtt tttgcccaat
    10021 tattttaatt actgattgac tagtagttgt catctggtta aaatctttta ttttacctat
    10081 attaacggcc aaaatagcat ttcctcgctt ataactctcc tggatttcat atttaacata
    10141 ttctcgatca agagtttctt tacctatcag caccacagtc actgatgttc caattaattg
    10201 attatctatc cagtttttta tagcaatatc tccattacgt ttaactttct caaattcagc
    10261 cttatctaca aaaccagctt cctttgttcc tcgcaaagtc cagctgtttc taactaccat
    10321 tgctcttgtt acatctgcat aatggaaact aaaaaaaacc cttctcacca tattaatcct
    10381 cctgaatact atttattaaa ctgacaattt gttcaacttt tttatctact tctccttcag
    10441 tatctaattt ttctagcgct tgaaatttac tttttaagga atcatcaaaa taatcactga
    10501 aattctcttg aatcttatca aacaaaacct tacttgcacc tttggtaaat ccaatcggaa
    10561 ctactatatt ttcattttga acagataatt caaattcttc aatcattcca tctgctgtaa
    10621 ctatttcacc tgattcaagt ttgtttccaa aaacaaaaat actaatccca gactctgcaa
    10681 taatgttttc tcgatatctt ctccactcat gaggcatatc aaggggaagt ggtcttaaag
    10741 aaagtacgtc ttgtatgtta ttttttctta aatttcttct ctgctttaga actcctgaaa
    10801 caatactatt accaattcca agtccgtaac ctgatactat tctataaccg gattcagaaa
    10861 gcctatatgc taatttctcg acaaatttta atcctttcga ttcagatgta aatggagtat
    10921 aatcaacagc acttcccgaa ataaaaatag tttttctcaa atactttttt ctaatacttt
    10981 tcaaaatatc agtaatctca gaataatcat ctacttcaac aacgtttatc gaaaatctgg
    11041 acaaatcatt gatttg
//
LOCUS       DAZUKD010000091        11003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000091 DAZUKD010000000
VERSION     DAZUKD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.093"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..2877
                     /locus_tag="ACG8N9_002069"
     CDS             88..2877
                     /locus_tag="ACG8N9_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4897634.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2924..3451
                     /locus_tag="ACG8N9_002070"
     CDS             2924..3451
                     /locus_tag="ACG8N9_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4897635.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3472..4662
                     /locus_tag="ACG8N9_002071"
     CDS             3472..4662
                     /locus_tag="ACG8N9_002071"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4897636.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4702..6000
                     /gene="asnS"
                     /locus_tag="ACG8N9_002072"
     CDS             4702..6000
                     /gene="asnS"
                     /locus_tag="ACG8N9_002072"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4897637.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            6930..7604
                     /locus_tag="ACG8N9_002073"
     CDS             6930..7604
                     /locus_tag="ACG8N9_002073"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4897638.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLIAQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7601..7942
                     /locus_tag="ACG8N9_002074"
     CDS             7601..7942
                     /locus_tag="ACG8N9_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897639.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSFFAQF"
     gene            7972..8331
                     /locus_tag="ACG8N9_002075"
     CDS             7972..8331
                     /locus_tag="ACG8N9_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4897640.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGISLIVNIIWLIISGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            8592..8714
                     /locus_tag="ACG8N9_002076"
     CDS             8592..8714
                     /locus_tag="ACG8N9_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897641.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            8856..10937
                     /locus_tag="ACG8N9_002077"
     CDS             8856..10937
                     /locus_tag="ACG8N9_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4897642.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         3662 a         1937 c         2406 g         2998 t
ORIGIN      
        1 tttctttaaa aagcgatgag agtatttttc tgcactttgt agtataattt caaaagaaca
       61 ttagaggcag gagaagagga agatattttg aaaccaagaa cttatgcagt cgttgatatc
      121 gaaacgactg ggacgaatcc aaaagaagat cggatcatcc aatttggctg cgtcatgatt
      181 gaatcaggtc ggatcacttc acgtttttcg atcgatattc atccagggcg gaagatttca
      241 aaacaaatcc agcatttaac agggatcacg aatcaacgcg tccaaaaagc gccttatttc
      301 gaagatgtcg cccaaacgat ttacaatctt ttggcagaca ccatatttgt cgcgcataat
      361 atttattttg attataattt tttgaaccat gaactcatgc gatgcggact tcctagtttg
      421 aagataccag gcatcgatac agtagaactt gcacaaattt tcttgccgac ggaaccaagc
      481 tttcgcttag ctgatttgtc agaaagcctt ggatttattc atgagaatcc tcatcaggca
      541 gatagtgatg ccgaagtgac aggtcaactt cttttgctga tcgaagaacg gatgagaaaa
      601 ttgccaatca tcacgttaga gcagattgcg cgattaagta gacacacagg tatggatacg
      661 agtcgtttca tttatcatgt gattgaagaa atgaaagaaa aaccagagcc acttgatccc
      721 tcattagaaa tcgtcgatgg gctagctttg cagaaaaaag aagtggaact attcacatcc
      781 gtccattatg gtgagcggac atatccaaga aaaaaacaag caaaagaaaa aatgtttggt
      841 aaaacactga tgtatcgaaa agaacagaac agactgatga atgcggtcta cgaccatttc
      901 acaaaagatg aatcaaaaga tttgatgatc gaagcagcta caggaatggg taaaacaatc
      961 ggttatctgc tgccattgag ttatttggct acacctgaaa aacccgctgt catcagtacc
     1021 gtttcgctag ttttgcagca gcagatcata gaaaaagaca ttccattgct gaatcaattg
     1081 ctagatcagc ccatacaacc tgtaattatc aaaagctatc gccactatat tgatttacag
     1141 cgttttaaag gcacacttgt ggaaccgcca gaacaaaaac aatatgcttt gtatcaaatg
     1201 gcggtcttag tttggctgac gcaaacgaag accggtgatt tggatgaatt acatttaaca
     1261 aatttgaacc attcattctt tgcagatatc gcgcacagag gtattggatt tttagcaaga
     1321 aatcagtcat tttacgaaca agactttgtt cgtttcttgc aaaagaaaat caggcaaagt
     1381 aattttttga ttgtcaatca tgcgttttta atacaagaaa cacagcgaaa agaaccattg
     1441 cttccagcta gtccattttt gttgatcgat gaagtacatc atttgccgga aatcgctgaa
     1501 aaagtaaatg acaaacgatt aagtattaac tatttccata aacaaatcca gcaattagaa
     1561 gaaccaaatc agctgtttga ccgaatacaa agctatttac cgaaagaaca tgaagcctta
     1621 cgctggttat cgatttatcg agaagaactt tctgcgctat gcgaagcaca gatttgtatg
     1681 gcagaaggat taatgagttt agtgagcggc aagcaaccta acggacagta tccagaagaa
     1741 gtcatgatga caaaagagat gttggatcag ctgccactgg aatctgggac agccattgat
     1801 cacctgcagc ttttttatca ggagatccag cagctacagt cacaaatgcg tctgactttt
     1861 gagattatcc aagaaagctg gacaaaccag atgcggatcg atttagcaag tttatatagc
     1921 ttatttgatc aattagacaa gcagtacttg ctgatggatc aatggctgga agactggcga
     1981 gatcatttga tccattggtt cgttcctggg aacacagcgc agcagatgat ttttcatctc
     2041 catgattttc aagcggctat gttgccgtcg actgtttggt atcctcgata tgatcgtatt
     2101 ctttatattg gaggaacgct gaagatcggc cctaatcgac actatctctc taagcggctt
     2161 ggcattgaag cagcacctct taaagtgatt gcctctcctt atgattatga gaaacaggca
     2221 aggatctatg tgccaaaaga ggcacctgcc gtccatcaag tcagcaatca agcatatatc
     2281 gattatttag agacagcact tacaggtctg atcgaacaag aagatcggcc aatacttgtg
     2341 ttattcactt ctcatgatgt tttgcagaag gtctatcaga aaatgcatct gtctatgcta
     2401 gaaaaaggac gagaaatact agctcaaggg tttggcggaa gccgggagaa gcttttgaaa
     2461 cgcttcattt tgtcagaaaa aagcgtattg tttggcgctg acagcttttg ggaaggtgta
     2521 gatttgcctg gagatgcttt gcagatcgtt gtagtcacac gtttaccctt cgataatccg
     2581 cttcgcccga tggtcaaagc taggaacgct tatttagaat cagaggggat caatcctttt
     2641 taccaggaat ccgtacctaa agcagctttg aagttacgcc aagcattagg tcgcttgata
     2701 aggacggaga cagatcgtgg cgtattgctt attttggatc gaagactagt cactgcaaaa
     2761 tatggtgcac ggatcatcca tgcattaccg aaaaaagtac cgatcaaaga gctagctatg
     2821 gaggaaatct atcaggacat ccatgaattc ttaaagaaaa atgaagaagg aagttagagg
     2881 agctgtcttt tttcataatt aaaatctgct ataatgaaaa ggaatgaaga taaaggagcg
     2941 acgacccttg aagaaaaaac agacaaagga aaaaacgatt aatcgcgtgt tacttggctt
     3001 gatcgtattt ttattagcgg tcatcgtgat cagctcgatt ttttacttgc gttccagtcg
     3061 tccggcggtg caggcgaaga aagaagcagt agaaatcgca aagaaatatg caaatcttga
     3121 ggaagccgat catttttatt ggtttactcg aaagaagaca tacttttcac tgacaggtac
     3181 aaatgataaa ggaaaagaaa tcgccgttat cattcctaaa tctggtgaaa aagtaaaagt
     3241 attggatcaa acaaagggac tgactgaaaa cgaagcaaaa cagacaattg ctgcggccca
     3301 tcctgaaatc caaatcgaaa aagctgcttt agggatgtat gatgatcagc ctgtgtggga
     3361 agtagtagga aaagaaacaa atgggaaact gaattattac ctgattggct ttgaatctgg
     3421 agatgaagtc aagacaatca agggaattta aaaggtaaag gaatgaatgg gatggagtta
     3481 tcaaaaagaa caaaacgatt agaaccgtct gtcactttgg cagctgcggc aaaggccaag
     3541 gcattaaaag caaaaggcag agatgtgctg agtttgactg tgggagaacc ggattttgcg
     3601 acaccagaaa acatccaaga agcagcaatc gaagcaatcc gcaatgggaa agctagctat
     3661 tatacaccaa cagcaggaat cccagagctt cgacaggcaa tcgttgatta tctgaagaaa
     3721 aactatggac ttgtctatga accttcacaa acagtcgtaa cagacggagc gaaatttgct
     3781 ttgtataccc ttttccaaac gatccttgac ccgcaagatg aagtcatcat tcctgtgcct
     3841 tactgggtca gttacggtga gcaagtaaaa ctggcagaag gacttcctgt ctttgtgaaa
     3901 ggggaagaat ccaatagctg gaaagttact gttgagcagc tagaacaagc acgttcagaa
     3961 aaaacaaaag ccgtaatcat caattctcca tcgaatccga ctggaatgat ttatagcaag
     4021 aatgaactgc aggcaatcgg tgaatgggca gtaaaaaatg atattttgat agttgctgat
     4081 gatatttatg gtcgtttagt ttataatggc aatgagttta ctccaattgc aacgatttca
     4141 gaagcaatca aaaatcagac catcatcatc aacggcgtgt ctaaaacata tgcgatgaca
     4201 ggctggcgca ttggttatgc ggtggggaat tctgagatca tcaatggaat gatcgctgtt
     4261 gcttcccagt caaccagtaa tccgacagca gtcagtcaat atgcagctgt tgaagcattg
     4321 aaaggcgagc aagatacagt ggaagaaatg cggaacgcct ttgaagaacg cctgaatacg
     4381 ttatatccgc ttgtggcaga actgccgggt gtttctcttg aaaagccgca aggagctttt
     4441 tatctttttc ctaatgtaaa agaaacgcta cgcatgtgta aatatgaaaa tgtcacacaa
     4501 tgggtagaag atcttttaga agaaacaggg gtagcgcttg tgactggcga aggctttggg
     4561 gcaccagaaa atgtccgcat gagttatgct actgatcgcg ctacgttaga agaagcggta
     4621 cgcagaatca agcaatttat tgagagtaaa agtcaaaatt aatcctgaag aaattcaagg
     4681 aatataaaag gagagtcata cgtggaacaa attcaaatca tcgattcgaa aaaccatgtg
     4741 ggagaaacag taacaatcgg cgcttgggtc gcaaataagc gatcaagcgg aaaaatcgca
     4801 tttttacaat taagagacgg aacagccttt ttccaaggtg tagttgtaaa aagcgaagtg
     4861 ccggaagaag ttttccaaac agctaaaaat ctacaccaag aaacatccgt gttagtaact
     4921 ggagaaatcc gtgaagacag tcgttcaaaa tttggttatg aaatcggaat tacaaatgtg
     4981 gaagtggtaa gcgagagcca cgattatccg atcacaccaa aagaacacgg aacagacttc
     5041 ttgatggatc atcgtcattt atggctgcgt tcttcaagac aacatgcgat tatgcagatc
     5101 agaaatgaaa tcattcgtgc aacgtatgaa ttctttaatc aacatcattt tgtcaaaatc
     5161 gatccgccga tcttgactgg ttctgcacca gaaggcacta cggatctatt cgaaacaaat
     5221 tactttgatc aaaaagctta tttatctcaa agtggccaat tatacatgga agctgctgct
     5281 atggctttcg gaaaagtctt ttcattcggt ccgactttcc gtgctgaaaa atcaaaaaca
     5341 cgacgtcatt tgatcgaatt ctggatgatc gaaccagaaa tggctttcac tcatcaagaa
     5401 gaaagtttag aagtacagga acaatatgta gcattcttag tacaaaatgt tttagatcat
     5461 tgcgaatacg cattagatgt actaggacga gacaaagaag tcttaaaaac ttatacaaaa
     5521 ttgccattcc ctcgtattac ttacgatgat gcaatcgaat tactgaagaa aaatggattt
     5581 gatgacatcg aatggggcga agactttggt tcacctcatg aaacgttcat tgcgaattca
     5641 ttcgaacagc ctgtctttat cctaaactat ccaaaagcca tcaagccatt ctacatgaaa
     5701 ccacatccaa caagagaaga cgtggtgatc tgtgcagata tgatcgcacc agaaggttac
     5761 ggtgaaatta ttggcggaag cgaacgggca acagactatg atttcttgct agaacaaatc
     5821 cgtaatcacg gtttggatga aaaagaatat tcatggtatc ttgatctacg taaatacggt
     5881 tctgttccgc actctggttt tggtctaggc cttgaacgaa cagtcacttg gattgctggt
     5941 atcgaccatg tgcgtgaagc tattccattc ccacgtttgt tgaaccgaat ttatccataa
     6001 gaggttgtga aatcagtgcc ttgacctgag tattagagaa acaaaacgga aaaatagctt
     6061 ctcatatttt accgtttttt tagctaatat cgagggtcag ctgattgaac accgtttata
     6121 tcaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
     6181 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
     6241 tattctaaaa aagaactgcg aactctcgca gttctttttt ttaaatacgg gataaaggaa
     6301 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
     6361 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctaattt
     6421 gatgatgctt ctgctgttga aataaagcat catctagcac ctgaaaacaa actaatagta
     6481 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
     6541 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
     6601 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
     6661 ttgatggcgt catcagtggt ctgattgacg gaagcttggg tatgatcgat ctctctggac
     6721 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
     6781 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctgatgt cattttttat
     6841 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
     6901 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
     6961 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
     7021 ttttgattgc cggaaaacta atcgcccaat atccaacgat ggaatttgaa tgcatgaacc
     7081 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     7141 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     7201 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     7261 aattactaaa tgaacttcaa acagcggaaa tctgcaacat catcttgatg gagccgtttg
     7321 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     7381 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     7441 atgctttagg gattgcttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     7501 cgattggtgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     7561 tttttagtgg agataaaaat gagcgaaaga aaagtgaaaa atgatggaaa taaggtgggc
     7621 agtaaaagaa gatttacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     7681 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     7741 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     7801 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     7861 agaaatccaa aaaaaatttg cagaatattt gatgctggaa gctaaaaaaa gaagaataaa
     7921 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatat aatgagttgt
     7981 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     8041 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     8101 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcata
     8161 tcattgatcg ttaatatcat ctggctgatc atttcaggcc taccaattgc tctcgcacat
     8221 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     8281 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaaagagaat
     8341 aataaaggtt agaaaacgca tagagtcagg tgggatacat gtatttcacc tgactctatg
     8401 tgttttcctg tataagttct cccatgtgac ttttacgtct tttatttata tgggattact
     8461 gtttatggaa agttgaaata attttcatta aagaactctt gctatatttt tgaaagctta
     8521 gtggagcttc gaatatctgg gaaggacatt ttgcagggat tgaaaaaaga tattgtccta
     8581 tcgaaaaatt aatgaattta gacaaattgc cagcaacagg cttcaaactt agttgctatc
     8641 ctgtcaagat caagaaggca agtgcgggtt ggataagagc agtggcaatg atcgaaaaga
     8701 aaaagaaaga ataagccaaa acatctgtat ggaagaaatt ttccgatgca gatgtttttc
     8761 attttttctt tcggatacct tgacatatga ataaccgttc atttataatt agttatgaag
     8821 agatattcat atgttgaggc gaggaggtca ttaaaatgaa aagttacaga ttagaaggtt
     8881 tagattgtgc gaactgtgca atgaaaatcg aaaaaggagt tcagaagatc aatggagtca
     8941 aggaagcaac ggtaaacttc acttctggaa aactaacgat tgacgcagag gaagatcatt
     9001 tggctacgat tgaacaagaa ataaaaaaag tagtgaagga attagaacca gatgtcaaag
     9061 taacagaaat agataaggaa aaagtatctg aacatggaaa tgaaaaagag agaaatacgc
     9121 tgttccggat tttattttcc cttgcaggta tcgctttatt gctattattt gattttaatg
     9181 aaccaattcg cttgataggg tatttattga tttatctgct gataggatat gatgtagtga
     9241 agaaggcagt gatgaatatc gtcaaaggaa aaatctttga tgagaatttt cttatgtctg
     9301 ttgctacgat tggcgctatg ttgatcggtg aatatccaga agccgtagca gtgatgctat
     9361 tttatcaaat cggcgagtat ttccaaggat tggcagtcag tcattcaaga aaatcgattc
     9421 gcgaattgat ggcgatccgt ccagaaacag ctcacgtgca gacgacggaa ggattgatga
     9481 ccgtgaaccc tgaagacgtg cagatcggtc agtttgtact tgtgaagcct ggtgaacgtg
     9541 taccgttaga tggaacgatc atcgaaggag aatcattagt cgatacgtca gcacttacag
     9601 gagaatcagt ccctaaatct gtatatgttg gtgaaacagt attaagtgga tttatcaata
     9661 aaaataaacc atttttagtt caagtagaaa aatcttatga aaattctact atcagcaaat
     9721 tattagaatt agtagaaaat gccagcagta aaaaagcacc agctgaaaat ttcattacta
     9781 aattcgcgcg ctattacaca ccagtcgttg taggattggc cgttttatta gctgttttac
     9841 ctccacttgt tgtttctgga gcggcgtttt ctgaatggat ctatcgagca ttgacatttt
     9901 tagtgatctc atgtccatgt gcattagtga tctccgtacc ccttagtttc tttggcggta
     9961 tcggaggagc aagtaaaatc ggtgtattag taaaaggttc aaattacttg gagttgcttg
    10021 cacaaactga gacagtggta ttcgataaaa ccggcacatt aacgaaaggt gacttttcga
    10081 tccagacgat cgatacaaaa atcgatccaa agatattcat gcaatatgtt gcgagtgcag
    10141 aacagttttc tacacatccg attgctcaat ctgttttaga aggctacgca ggtccgcttt
    10201 taccaacagc taatatccaa gaatttgcag gtgaagggat cttagctgaa gttgatggca
    10261 aacaagtatt agcaggtaat cacaaattga tggaaagatt tgaaatctct tttccttcaa
    10321 gtcaagaaat cggtacattg ctttatctag cgatcgatca aagttacagt ggttatcttg
    10381 ttattgcaga taccttgaaa gaagatgccg tagacgcatt ggttcaatta aaaaaggctg
    10441 gtgtgaagaa cacagtcatg ttgacgggcg attcgaaaaa aattgctgat catatcggga
    10501 agcaagtagg cgtggacaag atatatagcg aattgcttcc agaagataaa gtgcagcggt
    10561 tagaggaaat cttacaaaga aacaataaaa agaccgcatt tgtcggagat ggaatcaacg
    10621 atgcaccggt gctagcaaga gcagatgtag gaatcgcaat gggtggattg ggaagcgatg
    10681 ctgcaattga agcagcagat gtagtcatca tgaatgatca accatcgaag attgcagaag
    10741 caatccattt agcaaagaaa acattgaaaa tcgtcaaaca gaacattgtc tttgctattg
    10801 ggataaaaat tttggtactt gccttaggtg cttttggttt tgcatcaatg ggagatgcag
    10861 tatttgcaga tgttggggtg acagtattag ctgttttgaa tgccatgcga agcttgcatg
    10921 taaaaaataa acgttgatca atgaagagga cgaatgaacg tcctcttttt tttatatcct
    10981 tttcttaatg aaaaatgtaa aga
//
LOCUS       DAZUKD010000092        10791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000092 DAZUKD010000000
VERSION     DAZUKD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.094"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..1339
                     /gene="lysA"
                     /locus_tag="ACG8N9_002078"
     CDS             80..1339
                     /gene="lysA"
                     /locus_tag="ACG8N9_002078"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4897643.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1326..2126
                     /gene="dapF"
                     /locus_tag="ACG8N9_002079"
     CDS             1326..2126
                     /gene="dapF"
                     /locus_tag="ACG8N9_002079"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4897644.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2129..3010
                     /gene="dapA"
                     /locus_tag="ACG8N9_002080"
     CDS             2129..3010
                     /gene="dapA"
                     /locus_tag="ACG8N9_002080"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4897645.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3007..3723
                     /gene="dapB"
                     /locus_tag="ACG8N9_002081"
     CDS             3007..3723
                     /gene="dapB"
                     /locus_tag="ACG8N9_002081"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4897646.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3729..4424
                     /gene="dapD"
                     /locus_tag="ACG8N9_002082"
     CDS             3729..4424
                     /gene="dapD"
                     /locus_tag="ACG8N9_002082"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4897647.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4440..5441
                     /locus_tag="ACG8N9_002083"
     CDS             4440..5441
                     /locus_tag="ACG8N9_002083"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4897648.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5438..6799
                     /locus_tag="ACG8N9_002084"
     CDS             5438..6799
                     /locus_tag="ACG8N9_002084"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4897649.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6759..7910
                     /locus_tag="ACG8N9_002085"
     CDS             6759..7910
                     /locus_tag="ACG8N9_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4897650.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7885..9054
                     /locus_tag="ACG8N9_002086"
     CDS             7885..9054
                     /locus_tag="ACG8N9_002086"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4897651.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9229..10608
                     /locus_tag="ACG8N9_002087"
     CDS             9229..10608
                     /locus_tag="ACG8N9_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4897652.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3579 a         1896 c         2423 g         2893 t
ORIGIN      
        1 attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag
       61 tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt
      121 ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat
      181 gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc
      241 taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga
      301 tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat
      361 ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt
      421 gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg
      481 ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca
      541 tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga
      601 gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca
      661 tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt
      721 gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg
      781 gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat
      841 tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga
      901 accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa
      961 aaagacacca cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc
     1021 agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac
     1081 agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc
     1141 ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg
     1201 atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa
     1261 tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt
     1321 ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg
     1381 cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata
     1441 tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg
     1501 gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc
     1561 agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta
     1621 cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc
     1681 cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg
     1741 gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg
     1801 aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt
     1861 tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc
     1921 gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt
     1981 atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac
     2041 aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg
     2101 aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac
     2161 gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca
     2221 tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct
     2281 cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt
     2341 gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca
     2401 ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag
     2461 tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat
     2521 tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt
     2581 agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat
     2641 ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat
     2701 cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc
     2761 taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga
     2821 aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc
     2881 tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt
     2941 ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt
     3001 tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt
     3061 gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg
     3121 cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag
     3181 aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat
     3241 acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt
     3301 gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg
     3361 ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca
     3421 tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta
     3481 tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca
     3541 ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc
     3601 aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg
     3661 ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg
     3721 taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc
     3781 ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg
     3841 gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag
     3901 ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc
     3961 ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc
     4021 aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag
     4081 taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg
     4141 gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg
     4201 ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag
     4261 gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg
     4321 cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga
     4381 caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa
     4441 tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca
     4501 aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt
     4561 cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa
     4621 cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt
     4681 ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg
     4741 aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc
     4801 ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac
     4861 aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg
     4921 ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc
     4981 cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata
     5041 caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc
     5101 cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag
     5161 ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag
     5221 ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag
     5281 gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt
     5341 tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg
     5401 ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc
     5461 gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt
     5521 tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga
     5581 tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc atcatgttga
     5641 ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc
     5701 cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa
     5761 gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga
     5821 gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa
     5881 aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat
     5941 ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg
     6001 gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa
     6061 ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat
     6121 catcgaggaa atctctttta gagaactgag agagatggct tatatggggg ctaacgttct
     6181 tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac
     6241 caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg
     6301 actgacagga atagctggac gcaaggattt tctttccatc actttattca aacgtcatat
     6361 gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc
     6421 aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat
     6481 tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat
     6541 cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat
     6601 tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt
     6661 atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac
     6721 agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg
     6781 cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg
     6841 aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga ctactccttt
     6901 attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag
     6961 tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg
     7021 gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact aaacacattg
     7081 aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg
     7141 cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct
     7201 ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg
     7261 cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg
     7321 aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg
     7381 accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa
     7441 cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt
     7501 cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta
     7561 cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct
     7621 tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag
     7681 ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg
     7741 gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg
     7801 gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca
     7861 gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt
     7921 tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat
     7981 gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca
     8041 ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa
     8101 ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa
     8161 acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt
     8221 ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta
     8281 gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt
     8341 cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt
     8401 ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc
     8461 gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg
     8521 acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata
     8581 agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa
     8641 aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg
     8701 atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg
     8761 aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt
     8821 gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa
     8881 actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca
     8941 ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg
     9001 gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata
     9061 gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt
     9121 tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt
     9181 taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc
     9241 aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca
     9301 cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg
     9361 tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat
     9421 ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa
     9481 acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa
     9541 gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta
     9601 ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc
     9661 aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt
     9721 gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg
     9781 atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac
     9841 ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac
     9901 ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa
     9961 gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt
    10021 ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga
    10081 gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact
    10141 gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa
    10201 ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca
    10261 agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat
    10321 gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc
    10381 caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat
    10441 gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa
    10501 gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg
    10561 aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata
    10621 tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa
    10681 acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca
    10741 ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc a
//
LOCUS       DAZUKD010000093        10656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000093 DAZUKD010000000
VERSION     DAZUKD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.095"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            345..614
                     /gene="rpsN"
                     /locus_tag="ACG8N9_002088"
     CDS             345..614
                     /gene="rpsN"
                     /locus_tag="ACG8N9_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4897653.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            629..808
                     /gene="rpmF"
                     /locus_tag="ACG8N9_002089"
     CDS             629..808
                     /gene="rpmF"
                     /locus_tag="ACG8N9_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4897654.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            840..1217
                     /locus_tag="ACG8N9_002090"
     CDS             840..1217
                     /locus_tag="ACG8N9_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897655.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1238..1387
                     /gene="rpmG"
                     /locus_tag="ACG8N9_002091"
     CDS             1238..1387
                     /gene="rpmG"
                     /locus_tag="ACG8N9_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4897656.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1411..2394
                     /locus_tag="ACG8N9_002092"
     CDS             1411..2394
                     /locus_tag="ACG8N9_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4897657.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2424..2546
                     /locus_tag="ACG8N9_002093"
     CDS             2424..2546
                     /locus_tag="ACG8N9_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4897658.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2564..4087
                     /locus_tag="ACG8N9_002094"
     CDS             2564..4087
                     /locus_tag="ACG8N9_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4897659.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4111..4350
                     /locus_tag="ACG8N9_002095"
     CDS             4111..4350
                     /locus_tag="ACG8N9_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897660.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4543..5004)
                     /locus_tag="ACG8N9_002096"
     CDS             complement(4543..5004)
                     /locus_tag="ACG8N9_002096"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4897661.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5199..5417)
                     /locus_tag="ACG8N9_002097"
     CDS             complement(5199..5417)
                     /locus_tag="ACG8N9_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897662.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5535..7151
                     /locus_tag="ACG8N9_002098"
     CDS             5535..7151
                     /locus_tag="ACG8N9_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4897663.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7392..8732)
                     /locus_tag="ACG8N9_002099"
                     /pseudo
     CDS             complement(7392..8732)
                     /locus_tag="ACG8N9_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9170..9316)
                     /locus_tag="ACG8N9_002100"
     CDS             complement(9170..9316)
                     /locus_tag="ACG8N9_002100"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897664.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9355..9549)
                     /locus_tag="ACG8N9_002101"
     CDS             complement(9355..9549)
                     /locus_tag="ACG8N9_002101"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4897665.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9459..9791)
                     /locus_tag="ACG8N9_002102"
     CDS             complement(9459..9791)
                     /locus_tag="ACG8N9_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4897666.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10305..10496)
                     /locus_tag="ACG8N9_002103"
     CDS             complement(10305..10496)
                     /locus_tag="ACG8N9_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897667.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10590..>10656)
                     /locus_tag="ACG8N9_002104"
     CDS             complement(10590..>10656)
                     /locus_tag="ACG8N9_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4897668.1"
                     /translation="TRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3540 a         1689 c         2079 g         3348 t
ORIGIN      
        1 ggcgataaac cagttaaaca taaaagattg aataaaaagt tatagtttct ttcatattgc
       61 cgttttaagt agaggaaaag ctacttaaaa cggtatttgt gtttgattgg actaataaaa
      121 gtgaattctt atttttcaga gcctaaactg catattcttt taaatgattt gattatctct
      181 atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta tttcatgtta
      241 tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta
      301 cgatttgagg tgtctaaatc aaaataaaag gagtataaag gtatatggca aaaaagtcga
      361 aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtatgcagat aaacgtcagg
      421 aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat tcaaatccga
      481 atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg cgaaagttcg
      541 gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct ggagtaaaaa
      601 aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa catctaaggc
      661 gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt catttgacga
      721 agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact ataatggaaa
      781 gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag ggaatcaaaa
      841 tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt gaaacgaccc
      901 agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga attattactg
      961 aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat tactatgagg
     1021 accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa gagtatgacg
     1081 aggagttatt tcaatgtatc gatggttatt gggagatacc tgttgatatg aaagaaaatt
     1141 tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa attcaagcat
     1201 atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata ttattttgga
     1261 atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga ataaccctga
     1321 acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt ttaaagaagt
     1381 gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat cgtttctggg
     1441 tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc accctacccc
     1501 agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta
     1561 ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt
     1621 cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa
     1681 ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa
     1741 acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt
     1801 attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc
     1861 atggcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa
     1921 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
     1981 gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc cttagttttt
     2041 gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca ccatcaagta
     2101 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttactc
     2161 caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
     2221 attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga acaggttaat
     2281 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg aaaaatgttg
     2341 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
     2401 gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
     2461 ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
     2521 gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
     2581 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
     2641 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
     2701 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
     2761 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
     2821 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
     2881 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
     2941 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
     3001 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
     3061 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
     3121 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
     3181 gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac aagttcctat
     3241 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
     3301 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaattgc
     3361 aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc ttgaaagtct
     3421 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
     3481 agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
     3541 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
     3601 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
     3661 tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg agtacaaaga
     3721 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
     3781 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
     3841 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
     3901 tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa gtaaggcaga
     3961 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg aattgcataa
     4021 ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag aaatgatggc
     4081 tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt gtctatggat
     4141 tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag attaaatggg
     4201 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
     4261 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
     4321 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
     4381 ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag cgcaacactt
     4441 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
     4501 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
     4561 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
     4621 aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga aatgattgga
     4681 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
     4741 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
     4801 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac cagttttgcc
     4861 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggacg
     4921 gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat tttttgtatg
     4981 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
     5041 ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac taattgaaaa
     5101 gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact gaaaatcgac
     5161 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
     5221 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt ttttttcttg
     5281 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
     5341 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag tatagtaaga
     5401 tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
     5461 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
     5521 gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat cttggttcag
     5581 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
     5641 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
     5701 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
     5761 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
     5821 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
     5881 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
     5941 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
     6001 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
     6061 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
     6121 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
     6181 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
     6241 cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
     6301 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
     6361 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
     6421 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat aaaagtattg
     6481 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
     6541 aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt aataaactag
     6601 tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag ggttgtttaa
     6661 ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg aaaaagtcta
     6721 atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc aagataggcg
     6781 atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt gtattctcta
     6841 tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt cgaattgatg
     6901 gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg aattatcatc
     6961 ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga gttaaagaag
     7021 ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga
     7081 gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc acagattact
     7141 acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat attattatta
     7201 ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg
     7261 acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag tcggttagat
     7321 tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga ataatcgaga
     7381 tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc tttagtcagt
     7441 tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat tcgttctcgg
     7501 tagttttgaa agtttctata gccataggca attcgtttta gtactttgat atggttattc
     7561 aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat ttctgtttgg
     7621 tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt gtagtcttct
     7681 tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag aaaatcctgg
     7741 tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc gactatctct
     7801 gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc tttcaattcc
     7861 cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg acgatcccct
     7921 tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc aatgtgttgg
     7981 ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt caccaatgtg
     8041 cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac ttttataggg
     8101 tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat atcaatcagt
     8161 tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt tttcactgat
     8221 ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat ggttttaggc
     8281 cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat acgtgcgatc
     8341 aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac tctgcgagca
     8401 atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc acagtgttta
     8461 catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac atcattcaat
     8521 aagatggata cttttttcca gccctattta atgatcgttt gttcattttg atagtggcga
     8581 tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aaacaaaaca tgtttcgtca
     8641 ttgattcgct ctttgcgaaa ggcatcttca tgaaagataa gatttaggtc tagaatatct
     8701 agtaactctt tggtaaactc tgaataggac atcttgaaac actcctttga atggttttcg
     8761 tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa aaagaaaaag
     8821 tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt ttcttcgctt
     8881 caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg tagtagtctt
     8941 ctaaataatc ttggattaac accttcatct ctattttact tttatatttg ttggagtcga
     9001 ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca acaggagtcc
     9061 cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga taaataaaag
     9121 accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct taataaattt
     9181 gtaatggacg gacgacaatc tcttttttca tgtgtgaatc taccatatag taacgtagaa
     9241 tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta catggaatgt
     9301 aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat cgattcaaca
     9361 tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc gtcgatgaaa
     9421 tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt tattgatttc
     9481 gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat ctttgtgttc
     9541 gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat gtagaaatct
     9601 gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta tcgacgatat
     9661 tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc aattttcttg
     9721 gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt acgatcatat
     9781 gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag aaaagtgtat
     9841 catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat agataagagt
     9901 ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg attggacaca
     9961 tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca attctagagg
    10021 agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc ccacttcata
    10081 cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa gctaaaaacg
    10141 aattaaaaaa cgttgataat taaaatcatt cgttttagat tctatgaatc attatagaac
    10201 gctgtaaaat cgatacttta taattataaa gaagatagtt tcaaatttat tgttcgaaat
    10261 ttgaaactat ctttttactt atgtcacaga ctcccttagt tgagttattc tttaatttcg
    10321 aatttcgaga tagctagata tattgcaagc atacattctt gataacaatc ttgatcatat
    10381 tttccagttc tccaggattg cttatgaatc aaagagctaa atcttttgag aattgcctca
    10441 acagcttctt catttccagc ctttgctaaa atgaaatctt cttttaaaga attcataaat
    10501 ctaccttctt cccatggact tataatctat ttctttggga aggaggagaa cgagcaaaga
    10561 agcatacaaa attagttatt atgcagatat taaaccagca ttttttgaat ttttttgtaa
    10621 agtcgatgtt ttaaatttgt cgcaccctgt cttgta
//
LOCUS       DAZUKD010000094        10641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000094 DAZUKD010000000
VERSION     DAZUKD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.096"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACG8N9_002105"
     CDS             <1..525
                     /locus_tag="ACG8N9_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897669.1"
                     /translation="LEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2055
                     /locus_tag="ACG8N9_002106"
     CDS             586..2055
                     /locus_tag="ACG8N9_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4897670.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2066..4175
                     /gene="topB"
                     /locus_tag="ACG8N9_002107"
                     /pseudo
     CDS             2066..4175
                     /gene="topB"
                     /locus_tag="ACG8N9_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            4191..4340
                     /locus_tag="ACG8N9_002108"
     CDS             4191..4340
                     /locus_tag="ACG8N9_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897671.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4421..5017
                     /locus_tag="ACG8N9_002109"
     CDS             4421..5017
                     /locus_tag="ACG8N9_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4897672.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            5030..5929
                     /locus_tag="ACG8N9_002110"
     CDS             5030..5929
                     /locus_tag="ACG8N9_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4897673.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            5932..6416
                     /gene="ssb"
                     /locus_tag="ACG8N9_002111"
                     /pseudo
     CDS             5932..6416
                     /gene="ssb"
                     /locus_tag="ACG8N9_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            6438..6695
                     /locus_tag="ACG8N9_002112"
     CDS             6438..6695
                     /locus_tag="ACG8N9_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897674.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(6854..6988)
                     /locus_tag="ACG8N9_002113"
     CDS             complement(6854..6988)
                     /locus_tag="ACG8N9_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS4897675.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            7131..7391
                     /locus_tag="ACG8N9_002114"
     CDS             7131..7391
                     /locus_tag="ACG8N9_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897676.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            7840..8046
                     /locus_tag="ACG8N9_002115"
     CDS             7840..8046
                     /locus_tag="ACG8N9_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4897677.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            8046..8297
                     /locus_tag="ACG8N9_002116"
     CDS             8046..8297
                     /locus_tag="ACG8N9_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897678.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            8313..8750
                     /locus_tag="ACG8N9_002117"
     CDS             8313..8750
                     /locus_tag="ACG8N9_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897679.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(8861..9088)
                     /locus_tag="ACG8N9_002118"
     CDS             complement(8861..9088)
                     /locus_tag="ACG8N9_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897680.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIIFFKWITF
                     FLLSYRALPVYFAPPKRRGRLVRNKNHSLSY"
     gene            9186..9374
                     /locus_tag="ACG8N9_002119"
     CDS             9186..9374
                     /locus_tag="ACG8N9_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897681.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            9492..9698
                     /locus_tag="ACG8N9_002120"
     CDS             9492..9698
                     /locus_tag="ACG8N9_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897682.1"
                     /translation="MKYNLLDHKRMWVEKLEEDLEQLELLGHNKTSTVYKEGLKRTKK
                     AQKELATLESRVQKKEVDLTHTYL"
     gene            9708..9941
                     /locus_tag="ACG8N9_002121"
     CDS             9708..9941
                     /locus_tag="ACG8N9_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFS4897683.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            10263..10466
                     /locus_tag="ACG8N9_002122"
     CDS             10263..10466
                     /locus_tag="ACG8N9_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS4897684.1"
                     /translation="MILGIVLLSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSE
                     IVDRGVKAYVNGFTKVLGGVGGR"
     gene            10456..>10641
                     /locus_tag="ACG8N9_002123"
     CDS             10456..>10641
                     /locus_tag="ACG8N9_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897685.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
BASE COUNT         3880 a         1739 c         2233 g         2789 t
ORIGIN      
        1 ttggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt
      241 cctgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt
      361 ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatt cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca
      841 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      901 gagacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac
     1081 cgtttagatc atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg
     1141 gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa
     1201 cacattgaca agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag
     1261 catttaaaat tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag
     1321 aaactacaag atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat
     1381 tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa
     1441 atatgtgtgg acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga
     1501 atcgttgatc cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg
     1561 caagtaccta aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa
     1621 cctgaagcaa ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg
     1681 gtgtctgctc atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta
     1741 gaaacaattg atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa
     1801 cgcgcagatg gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac
     1861 gttaatgcag gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag
     1921 catgaacatg aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag
     1981 atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa
     2041 caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca
     2101 agcgaaggct tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat
     2161 ccaagatcga ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga
     2221 gttagattcg cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc
     2281 tattttcccc aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt
     2341 cgtcaaacga cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga
     2401 aggggagctg attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt
     2461 taaaagactt tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt
     2521 acgagacgca gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga
     2581 ttggttaatt gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat
     2641 accaggaagt ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt
     2701 gcaggaaaag attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat
     2761 cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga
     2821 agcatttgaa gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat
     2881 ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag
     2941 ccttcagtct aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat
     3001 gcaagggttg tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac
     3061 ggaaggggaa tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc
     3121 cgaagcgatt ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt
     3181 ccaggaacat tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca
     3241 actaagtcca ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt
     3301 tgcggaaaag tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt
     3361 aaaggcaatc gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag
     3421 cgacggaaaa gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt
     3481 gacggtggat ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg
     3541 tacgttgatc actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc
     3601 catattgaaa gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac
     3661 cttgaaacaa aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg
     3721 gattttgctt tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc
     3781 caaatgggaa agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct
     3841 agccaatatc caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc
     3901 cactgatttt ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc
     3961 gcgtcattta ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt
     4021 ggcagcttgt acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa
     4081 cgattgtttt aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga
     4141 acttcaagaa aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata
     4201 ccacacacaa acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa
     4261 aaatgcgtgc cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag
     4321 aaatcgaatt agatcgataa aatcttccta actattgtat acgtataaac ttatgttata
     4381 ctgtttttat aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata
     4441 caagctgcta gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat
     4501 ttctcctgta acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg
     4561 ttttttcacc ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga
     4621 acgcggcatt atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc
     4681 tagacagatc gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga
     4741 attcgcagtt gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat
     4801 aaaaagtcgc tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat
     4861 ttggaatccc tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat
     4921 ggagtcggac agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga
     4981 caaaacgatg cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac
     5041 gcagttaaaa gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt
     5101 tttaagacat tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga
     5161 aaattttgtg ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac
     5221 aaccaaagat attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac
     5281 ggttctaaac gaaatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg
     5341 aatgaaagaa acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca
     5401 tttggaaaac atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc
     5461 tgcaacgatc actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc
     5521 ctatcctaca gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga
     5581 taattcgaga ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga
     5641 tgagaagacc ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc
     5701 gttaaagata tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga
     5761 tacctggaag aaatatcaaa aaagcaatcc ttatgctgca tcgatcacgt acgatgagac
     5821 aatggattcg attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcaaaa
     5881 actagcttat caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat
     5941 aatgtgacat tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt
     6001 actgcagtag cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat
     6061 cgagaagccg attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca
     6121 ttagctaaaa aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac
     6181 aaccagcaag gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta
     6241 gaaaataaag cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc
     6301 accgccacct ttgaacaacg gatacagcga cgcctaacaa caatggattg aacgcttcac
     6361 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttagtttg
     6421 agaaagaagg agtaagcatg gcaaaaaaaa ttagtctgac gatggatcta ttgatacccg
     6481 taattgcatt tatagacggg atttctttgg gcgtcactga aaatcctttg gtaaatagaa
     6541 ttgcgaaagg atcactattt cttgtattcg tgtatcttgt ctgttcttat cttttaaaaa
     6601 gagcaggaaa ggataattcc ttttggcgat tgactaaagt cacagtagca gcgacagctg
     6661 ttatacttac ggctattcta ccgctttata tgtagagtta acgggaaatc gttgaacttc
     6721 ttgcgaaata ttgctataat gaacatgaaa aagaggagtt gcgctaacaa cccctcatta
     6781 acagcaccga ttaaagaacg gtggcagaat attagttatt tggaaataac cgctgacctg
     6841 ctaaagtaga cggttatttc tttttgtcat ttttaagcat atttacaatc aaactaatca
     6901 aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat gcggacaaaa
     6961 ggcttactcc ttttatagat tttagcactt acattcataa gcaccaccac ctttccagaa
     7021 gtcgccaccg tcttcaaact ttactgtctt gactattata gcatatctaa aatcgaataa
     7081 aaattctcaa ttttaactgt ttttgctata attttagcga ggtgatcgtg atgaacaaat
     7141 atgttggttt gcttgataaa atccgagtaa taaaaacaca accattattg gttcgcttta
     7201 ctttacaaac aattcatgaa tcgattaatt gtgtagttgc agatattgaa attatcgata
     7261 aattgttaat tatggatgat ggaaaataca atattgccgt cacaggtcat ttcaataaac
     7321 ggaatcaatt agtcattgaa tcgatgcaaa ttagaaaccc tgatcatttt acgcgatcaa
     7381 tgggcatata aataaaagaa gagctgcaat cctaatgtag gactgcagct ctttttgttt
     7441 tacatagaaa caggaagaaa gggaaaggcc tgtttaggaa ggcacaagtg ccttctcaat
     7501 tgaaaaacct ttctctcact ccaaccgcca ccgccgttcg agaagaaagg aagtgaacgt
     7561 tgcacgttca ctaagataag gggtcttgct ttgtaagaca aaataggaaa agcttcctct
     7621 caccaaaagg ttcgttcctg ttcgctatct ctttattgat ttgccagacc ggcaacaagt
     7681 tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc gcgcccttgt
     7741 ccttgtcgct tttcgttgat tcattctggc caacaaatca ataaagaaag aactaacgaa
     7801 aggaagtaaa acctttccta agaggaggca gaatcaatca tgcgaaagaa atttgattat
     7861 ttgggtgttc ccttcagcga attattccca aactatcatg caccacatac tgtcgaatgc
     7921 gattgtggag aaagagcgaa atgtatcaaa agctattgtt tgtatcaatg tccaacatgc
     7981 gggaaaaaat ataccttgtc ttatggcgat tatattttga ttgaggagaa aggaaagaaa
     8041 cgctgatgaa aacacaacac aaattattga ctgtaaaaga agtcgttcaa attttggtgg
     8101 acaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     8161 tagaaacccc tttagtagag gatgcaccga ctatgctatt tgaagaccta gtgaagattg
     8221 caggatgtga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
     8281 ccaaattaga gaactaaaaa ggagaaaaaa atatggctca aaattactat gacgaatttg
     8341 tcaaattacc tttggataaa atggcacaaa aaatggagga tatgacgttc ctatacaacg
     8401 aaacacgtgt tccaaaaaaa cattataaag aaaaattgtc ggtagctgtg gaagaaatga
     8461 ttgaatcagg tgtcgaaatg aatcttattg cgacgtacta tcgaacactg gaagaactga
     8521 aaaagcaaaa tgcaaaatgg tttttccaag ccttactatg ctttgaaata ggtgtcaaac
     8581 caagcactat taaaccttct gaataccagg ctttagaact aacgtatgcg aaatttatcg
     8641 aaacgaaaaa agccaagact gtttcaagtg aatggttaga ttattttgaa aatatcaata
     8701 agtatggcgc atattacacc atgaaaaagg aagacaacga aaatgaataa ggtgatttat
     8761 caactaacaa cggattccat atcaacgatt tgaaagaaaa aaaggtcaat aatcatttat
     8821 accgaaaagt gagaatgtgt ggaataagga caaaaaaagc ctagtaacta agggaatggt
     8881 tcttatttcg caccaaccgc ccccgccgct taggtggtgc gaaataaacg ggcaaagccc
     8941 gataacttaa gagaaagaag gtgatccatt tgaaaaagat tattccctta ttgttagtac
     9001 tgtttgttgg cgaaatactt atgcgagata gtgcagctgt acagttaaac ctcgctgtat
     9061 ttttggtaat actggtgttt tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc
     9121 tactattcaa gatgattgca gccatatttt tcatggggtt atttcgatga aaggagagag
     9181 taccaatgcc aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac
     9241 aaaaagaatt gcagcaacta aagcagaagt acggcaaaag tgaatccagg attatgtgta
     9301 ttgcgttaga tttattgatt gcctgtattg gacaatatct ctattgtatt cgaggaagaa
     9361 agctttacgc ttgaatgcga taagttaacc cgatatatcg atccaaaatt ctatgaggat
     9421 aaaaacctgg catgggaact ggctttatag acagagtgct agtgcaagta aaggaagaaa
     9481 ggaagatcac catgaaatac aatttattag accataagcg aatgtgggta gagaaattag
     9541 aggaagactt agaacagttg gaattgttag gtcataataa gacttcaacg gtgtataaag
     9601 aaggactaaa acgaacgaaa aaagcacaaa aagaactagc aacactggaa agtagggttc
     9661 agaaaaaaga agtagactta acacacactt acctttagga gtagaaaatg gcagacaaga
     9721 gaaaagaaaa gattcaaaaa ttactggcgc ttgcggcgga tgtaaatgat gaggaatcag
     9781 tgagcgcatt agccaaagcc cagcaattga tgatggacta tgatcttact gaagatgaga
     9841 ttttcaatta tcatgagcag caacggatag aagcagtaga aacgcacgtg atctatcgtg
     9901 gacgtccgca aaaatggctg tatcgtttat ttagaaatta agataaaaaa ggctagttaa
     9961 caggtttgtt gactggcctt ttttgatatg tttgtacatg ttttattgtg ttttaatttt
    10021 tctctttttt atagcaagct ccttttataa taaatattcc gaaaagtgaa gaccattttt
    10081 tatttattaa gactattatt attagtgaga taaaatttga ttgatcttac agaatattgg
    10141 tgacgattat gacactaaag atactattta tgaaaatttt gcaaaaatgt tctgaaaatt
    10201 acatataatt gttttataaa aaagggaggc aattatatga agaaaaaatt tgttagtatt
    10261 ttttgatttt aggaattgtt ttattgagtg tatctacttt aggaattaca gtagatgctg
    10321 caacttatta tggaaatggt gtttattgta atactcaaaa atgttgggta gattggagta
    10381 gagctcgctc tgagattgtt gatagaggag taaaagctta cgttaatggt tttacaaaag
    10441 tgctaggtgg agtcggtgga agataaaaaa gaaaaacttt ttgacttaat tgcgaatgca
    10501 tataattcaa agtcatataa taatgtagaa gtaaaagaag gatttcttca agatgctaag
    10561 aaaatacgag aggataaacc atatcctgaa attattatat cagtgtacaa acatttatct
    10621 aaattaaaag ctgaggaacg c
//
LOCUS       DAZUKD010000095        10619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000095 DAZUKD010000000
VERSION     DAZUKD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.097"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..2237
                     /locus_tag="ACG8N9_002124"
     CDS             291..2237
                     /locus_tag="ACG8N9_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897686.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2240..2695
                     /locus_tag="ACG8N9_002125"
     CDS             2240..2695
                     /locus_tag="ACG8N9_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897687.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2709..4037
                     /locus_tag="ACG8N9_002126"
     CDS             2709..4037
                     /locus_tag="ACG8N9_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4897688.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4071..4355
                     /locus_tag="ACG8N9_002127"
     CDS             4071..4355
                     /locus_tag="ACG8N9_002127"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4897689.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4357..4872
                     /locus_tag="ACG8N9_002128"
     CDS             4357..4872
                     /locus_tag="ACG8N9_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFS4897690.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4888..5550
                     /gene="rpe"
                     /locus_tag="ACG8N9_002129"
     CDS             4888..5550
                     /gene="rpe"
                     /locus_tag="ACG8N9_002129"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4897691.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5557..6129
                     /gene="hxlB"
                     /locus_tag="ACG8N9_002130"
     CDS             5557..6129
                     /gene="hxlB"
                     /locus_tag="ACG8N9_002130"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFS4897692.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6316..7836)
                     /locus_tag="ACG8N9_002131"
     CDS             complement(6316..7836)
                     /locus_tag="ACG8N9_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4897693.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8079..8264)
                     /gene="tnpB"
                     /locus_tag="ACG8N9_002132"
     CDS             complement(8079..8264)
                     /gene="tnpB"
                     /locus_tag="ACG8N9_002132"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4897694.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8248..8430)
                     /locus_tag="ACG8N9_002133"
     CDS             complement(8248..8430)
                     /locus_tag="ACG8N9_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897695.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8671..8889
                     /locus_tag="ACG8N9_002134"
                     /pseudo
     CDS             8671..8889
                     /locus_tag="ACG8N9_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9384..9983
                     /locus_tag="ACG8N9_002135"
     CDS             9384..9983
                     /locus_tag="ACG8N9_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4897696.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10047..>10619
                     /locus_tag="ACG8N9_002136"
     CDS             10047..>10619
                     /locus_tag="ACG8N9_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFS4897697.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3509 a         1670 c         1984 g         3456 t
ORIGIN      
        1 tattataata aaaaaatacg aattttagtg acgccattat attgttttta tttttcaatt
       61 gtaatatttc atattacttc cataataagg agtattttct attggaatat cttagctctt
      121 ttaaggattt ctcctttttt gtttctcatt ttttaagatt atttggattt cctttacaac
      181 aaacttccac aagtattatt gacatagatg taattgaggg aagggggttc cagtgctaga
      241 ataaataatg aaaagaggta acaaatgcta gcgttaagag gtgaacaagt ttgattgaaa
      301 gacatgtaaa tttattaaaa ttattaagtc gacaagaaga ctttaaaccc gcttcttttt
      361 ttagtgataa attggcagtg tcgaccaaga ccatttatca agatattgaa ttgttggaag
      421 attatgtagt taattaccaa gtaacaattg agcgattacc ttctttgggt atcaaactca
      481 taggtgatag gaaaaacgtt gaacatttga tttcatcggt agagaattat cttgaaaaag
      541 atatttattc tccagaaaaa agacgtgtcg agatcattaa agaagttttg tttggtgata
      601 agcctgtttc tttaactcat ttgtctcaaa gttttatggt tagtaaaaca tcgttataca
      661 atgacctgag aattatcaat aaaattattg gcagtggaga agttaagttg cagtcatctg
      721 cagaaggaat tagttttact ggtaatgaac aagcaattca acgagcaatc aagcaacttg
      781 tattctatta tgcgaatgac atggaatcaa aaacattttt tcaagtttta aatatgttgt
      841 ttgattcaaa agttattagt gtcattcatg atttgctatt caatgattac agtgaactga
      901 ctgaaaaggt ttctgattac tatgttcggt cattgatttc tactttagtt atccaatgta
      961 accgtttact gaaaggtcat catttaactg ttgaagaaga attcctcttc aatagcattc
     1021 gctatatgga aacgtacatt gtggcaaatg gtttaattga agaattgaag gcaaaattga
     1081 atcttccatt ttatgacgca gataaagaat atctatgtcg tcaactattt gcacatcgtg
     1141 tgacaaataa tttaaaaacc agcaatgaag agtacgctat gagcgttcat aaattgattg
     1201 aaagaatgag tgagattgaa aaaattgatt tgactcaaaa tgatcgatta tatcaatcct
     1261 tattgtatca tgtgccagca atggtccttc gtttaaaaaa aggtattaaa attcaaaatc
     1321 cgttattaga aaatatcaaa tctcaatatt cagaactctt tagcatagta tggtacgcca
     1381 tgtccatcct tgaagaagaa tttgatgtga ctttaacgga cgatgaagtt tccttaattt
     1441 tgattcattt tcagattgct attgaagatc agtccaaagc aaataatatc atcattattt
     1501 gccaatacgg tatttcttct gcgcaattaa tttacaataa agttcgacgg tttttaccag
     1561 caaaagataa tgtagaaata tcaactttag ataagtttca tttagctgaa attggggaag
     1621 ttgatttgat tatttcctct ctttcctttg atgcaggtgg tataccctta gtaagggtat
     1681 ctccattggt aagtaaaaca gattatatga atattatgga agcttatact aaatatttaa
     1741 tagaagaacc ttcgcgattt attcaaaatg aactgattcc taaagcggtt aaagttccca
     1801 ccatatctaa atttgttcaa tcatctttag taaaaacagg agtacaggtg gaaagcaaag
     1861 aagaatgttt ggatttgatg atttccgaac tagaagccaa ttcattagtt actgaagagt
     1921 ttcgtgcttc tatcttctct agagagaaaa tggggaatac taatttagaa agtggtgttg
     1981 ctttaccgca tgcgaatcca tcaatggtca ttgaatccaa catttttatt gtagcattaa
     2041 aaaatgcgat taattggggc gatcgaaaag tcaaattagt cattatggtt agtttagctg
     2101 aagaagatgt atctgagatt agaggagtat atgaagaact ttaccaattt attgggcaaa
     2161 aagaaatggt cgatcgcttg ttacaagttc atgatagcga aaaattacta agaatttttc
     2221 aacaaaaagg agaataaaaa tgtatctaga taaagaatta tgtttatttc aattagaagt
     2281 gaaagatcaa aaagaattgt ttcaagtaat gtctgaacaa ttaaaaaacg ctggttgcgt
     2341 gaaagatagt ttcttagaag gtatcaccaa ccgtgaacaa gaatttccaa ctggattaga
     2401 agtaaatcaa attgggtttg ctatcccaca tacagattcc gcacatgtaa atagctctca
     2461 aatctgtttt gcctcattaa aggaaccact ggtattttca gatatgacag ataaatctca
     2521 tgaaatacct gtacgtttag tctttatgtt ggcaatgtca caacctcatg aacaaattga
     2581 tacactacaa aacttagtta gcctatttca aaatgaagaa aaagtaaatg aattacttgc
     2641 atgtaccacg aaagaagcat ttattgagtt agtacattct gcaggagttt attaggagga
     2701 atagagagat ggatattgta ttaaatgcct ttgaatggtt tattggatta gggtcaaacg
     2761 tctttttacc actgattatt tttgtaatcg ggatattatt tggattaaaa cctggtaaag
     2821 catttgtttc cggcgttact gttggtatcg gttctattgg tttaggatta gttttaagtt
     2881 tattatcaga tggtttagga acagcaattc aacaaatggg ggaacaatac ggtggtggcc
     2941 tgaatatcct agatattggc gtaggtgttg gtggaccttt ggcttactct acatcattgg
     3001 gaattttaat gattccaatt tcattaattc ttaacttcat atttgtggtt ttgggttgga
     3061 caaaaacatt gaatgttgat atttggaact tttggttcgc aattttccta ggtatgatta
     3121 ctcaatctgt tacgggaaac ttttggtttg gcttaattgg cgcgattgtc gcagtaattt
     3181 tacaatggtt cttagcagat cgtacacaaa aaactgttag cgaatttttc ggctatccag
     3241 gaatctctat cacacatatg atgggattat caggagtact atttgccaaa ccagtaaatt
     3301 gggtttttga tcgtattcct ggatttaata aaatagatgc agatgcagaa acattaacaa
     3361 aacgtttcgg tgttttaggt gacacagttg ttattggcct aattatcggt attgtagtgg
     3421 gtattatggc aggctatgac gtagcaggaa ttggaaactt aggaattatg actgcggcag
     3481 tgatgaagat tatgcctaaa atggtagcaa tgtttatgga aggtttaatg ccaattgcag
     3541 aagcggcgaa agaatttacg aataaaaagt taaatggtcg ccaagtaaac attggaatgg
     3601 atgcagcgtt aactgtgggt catccaacta ttatgtcaac ttctctattg cttgtaccaa
     3661 tctcattatt gctagcacta attcttccag gaaataaagt attacctttt ggagatttag
     3721 cattctttgc tttcgccatc tgtttaatga ttccttattt taaaggaaat attgtgagat
     3781 cattagtagg atgttcgttg tatgttgtta caacattata tatggcaaca tggttggcac
     3841 caattatcac tgatgtgttt acattagcaa gtttcgatgt ggggacagca ggattagtaa
     3901 ctatgttgtt atgtggatta tggccagtag ggttgtttgt tttattagca tcttactttg
     3961 gtgtaattgg aataggtgga ttaggagttg ttttagtagg tgcttctatc tacatgaaca
     4021 aagtgaagaa agcttaatca taaaattaaa ataagaaaga agagatcaat atgaaaaaat
     4081 tattagttat ttgtggagca ggtcatgcta cttctacaat tgcagtagca aaattatcaa
     4141 aatggttgga agaagaagga ttaacagatc aagtgaaaat ttatcaatct aaaattgctg
     4201 atgagctaaa taaaattgat gaatatgatg ctgtagtaag tacaacaatt gtccctgatg
     4261 taattaaagc taaagtaacg aatggcttgg gcttattaac aggtattggt acagatgcca
     4321 tttatgcaga tgtaaaagaa agattaggtg tttaatatgc gttttcatca tgacactttg
     4381 actattgaaa cagtttcagg gcgtccttcg tatcatgata ttcgaaaaga acttttagat
     4441 acagttgata cagcaggtat tgagaatgga atgttagtaa tttcgagccc gcatacaact
     4501 tgctcactct tttttgaaga aacgatgcat gacacaaact tttttggtga tgattattta
     4561 catgtagaca tcaacaatgt aatggaaacg attattccaa aaatgacgag tgaaaatcaa
     4621 tacaacagtc cggggccaaa acacattgat tttggaacaa gattatctga tcccaattac
     4681 cctgcagaaa aatgggtcat gctaaatacc gatgcacatt tacgttcatc tatttatgga
     4741 accaactcaa tgactttaat tgttcaagaa ggtaaacttt tattaggaga aatgggccgt
     4801 gtctattttg tcgattggga tcaactaaga gagcgaacta gaacaatcaa tctcatgatt
     4861 atgggagaat aaacggggga atagagaatg acaattgctc catcattgtt aaatgcacct
     4921 atcgcagaag ttaaagagtt atttgaaatc ttaaaagaaa agaatgttga ttatttgcat
     4981 attgatgtaa tggatggctg ttttgtacca gatatggctt ttggaccaag tgttattaaa
     5041 gatattcgtc cactaacaga cgttgtgttt gatgttcatt tgatgattga tacaccagaa
     5101 aaaattgtca caagatttgt agaagcagga gcagatattg ttaccttcca cattgaagct
     5161 accaaacacg caatgaaggt cattcagtta gtcaaggaag caggagcaaa agtagggatt
     5221 acttttaatc ctgcaacacc agtcagtttt attgaaccag tattatcttt agtcgatcaa
     5281 gtattagtaa tgacaatcaa tccaggaaca gagaataagc attttattca agagacagta
     5341 gttaaaatcg aacagttaga tgtaatacgc aaacaaaatg attatacgta tgatattgaa
     5401 gtagatggca agattgataa tcaaacgatt aaagtttgtt ctaaagcagg ggcggatatt
     5461 tttgtatcag gcggtttcat cttcaatggg aaaaataccc cgcaagaaca aattgataaa
     5521 ctaaatcaat cattagagga agtgaagtaa aagatcatga ccaataaaga ccaaaataaa
     5581 aactatttag aagcgattat cttagaatta caaaataact caaaacgtat taattctgta
     5641 gagttagaac aatttagtga gaaaattgtt caagctaaac acatctttct agctggtgca
     5701 ggacgctctg gaattgccat gcaagcattt acgaatcgct taatgcattt aggtttttca
     5761 gtcagccttg tcggtgaaat tagttcacca cattcgcaac ctggagattt attaattatc
     5821 tgttctggct caggtgaaac aggtagttta aaaaatttag cggccaaagc aaatcaaagt
     5881 ggtgtaagca ttgctttagt taccatgaaa aaagactcgt cgattggtca attagcagat
     5941 tctgttcttg tattaccagg tattaccaaa gatgaaaatc aacgagaaga agatactttt
     6001 tcacaaccta tgggttcaag ttttgaacaa ttagcatttc tcacctttga tagtttagtg
     6061 ttatctttaa tggataaaac aggtgaaagc tcagaaacga tgtttgctcg acatgcagat
     6121 tttgagtagt agattttaaa gataaaaaac cattctctaa atgctaatta ttcagcaaat
     6181 atgagaatgg tttttttatt tgattagaca taacttaata attcagtagt tttgagcgta
     6241 agcgtgtcat agaatggtac ctaaacacaa atagcccgat ttgatacact caaatcaggt
     6301 tatttgtggc ttatattatt tacagatttc ttgtatctgc tgattccatg gcaaataagc
     6361 gcgtagcgct tcaggtgtca tcgaagacag attgggaatc tctgtaaaaa gatgatttaa
     6421 atatttgcgg atgtttaacc cattcgcaac agctgtctgg ataatactca atataattcc
     6481 atttgatcta gcgcctttta agcttttaga aaatagccag ttttttcgac cgatcactaa
     6541 ctctttgatg gcacgctctg ctcggttatt tgaaagttcc aagcgtccat caagcaacac
     6601 aatctgaagt ccgtctttat gattgactgc gtattgaacc gcctttccaa gctgcgattt
     6661 tggcaaataa gcaaaactct cacaccaatg aaaaaattca gtcatcacag gctgcaacgt
     6721 ttccttcctc gctacaaacc tttcttctgc gctaagctta ttaaaggtcc gttcgattcg
     6781 aaacatcttg tcacagaagt ctaatcctat tttggcatga ctttttgggg acggttgttt
     6841 acccaatgct tcaaaaaact ttctgcgaac atgcgcccaa caataaacat tgtttaccgg
     6901 cagctttcca taggccgcgt aaccatcagt atgcaagaaa ccgctaaatg attctaaaaa
     6961 actttttggc tcatcaacgg aacgcgaatt tgcgtgctcg tagagcacaa cttgtttttc
     7021 ttcgctttta cctgaagcat ataaccagta aaaggtcttt tctttatgag tattcaatac
     7081 ccgatagctt gtctcatccg cgtgtaagac ctcctgattg cttaattctt gatgaaggca
     7141 attgtagatt ggctctaaaa ccatttcact taccttgata tgccaattag tgattttttt
     7201 acgatcaaga gggaaaccaa gtgcatgcca ttctttttct tgtcgatatg ccggcacctt
     7261 tttaacaaat ttttcccaaa tactctgtgt cacaattgaa gaggaaccta gactattatt
     7321 gatcaacggt ttaggaacag gcgctttttt gatttttgtt tctcctaatg cttcacattc
     7381 tctgcaatga taggcatgtt ggtagatgac ttgtttttct atatgggcag gaacatacaa
     7441 tggctcttct ctaagccttg tcgcaccgat atcatgtaaa gtatgaccac agtcttcaca
     7501 caaacaatcg gcacctttta attcataatg aataggaact tcaggcagac cttctaatga
     7561 aagtgctttg agtcccttgc gttttctgcg tttaaaagaa gataccgtaa tgggttcctg
     7621 gctttcttgt ccagtgtgct ctggttcatt aaaagttgag tcgtctaata aatcgatttg
     7681 cccatctacg ggtggtgttt ctttttttcg tccaaacaag cgtcgattca tttcagcaat
     7741 ttgttctcta agaagagtat tttcctgttg ctggatttca agcaactgtg ttaattgctt
     7801 gatttgtaca agaagttcag attccatttt tgtcattttt acaccaccat tctagcgatt
     7861 ttcttacatc tattatacca ttttatgaca acgaaaaaca gacaaaatcc ttgatttaac
     7921 aaggttttcg tctaatatat cgcacctttt ttcgtttctt ttagcgtaca tttttcttcg
     7981 attgaaaggc cagtcagtaa gacttctttt ctggaacgcg gccattgaag gtacccactt
     8041 tcaaatcttt tgtacattaa tacaaatcca ttttgatcct agtaaagagc cttgaagcga
     8101 tctttcttcg taccgcaaaa taagaaaagc gcttgatcgt aaacatcaag gtcatattct
     8161 tctataacga aagctgctag tccatcaatt ccttttcgaa gatctgtttt tccacaaacg
     8221 ataaaaatat gtttcaatgc agttgcatca atgagcattg gcattcaact cttttaacaa
     8281 ggtatacaaa agatatttat ccattccatt ataaataaat atttccttat cttccgattt
     8341 gattctgcaa gctagttttg gcttggtgtc aatttgcgat ttttgagccg cacttgaaaa
     8401 ttgaactggt acaatatctt ttttattcat tcgtttttct ccttctatat ttgatagttt
     8461 aattatagaa gaaagataat cggcgtacga gatactatcc tatgacacgc ttacatttaa
     8521 tttaatattt tgcctcaaat tagccacact ttttatagaa ctaagaggaa aaatgaagtt
     8581 ttcttcctat taatatagtt gtttgaaatc ttaaatatag cgttttacga tgtaatggga
     8641 agtatggtga ataaatcaac tatgttcgta cgaagcattt actcaactaa tttaatcgaa
     8701 tctttcaata aacaaatcaa gaaatacagc caccgcaagg aacagttcca aaatgaagag
     8761 tcgatggaac gtttcttagt ctcgtctttt gatacttaca accaaaaatt cctaggtcgc
     8821 agtcataaag gtttccaaca ggccgaaggc gagctcgaac aaatgctgag tcaaccgatg
     8881 gagaattaga aacaatctac agtagggaag aaccattgac acaaaattat tgacgctccc
     8941 aactaaaagt gtcaaaataa tgatgaaatc atttagttct tataaattag aaaagagaaa
     9001 ataaatattc catatctact ttattggatc cgtaacatga ctaaattctg aatacttttt
     9061 aaagcttatc atttggtaaa actgaatata agttaattat atagactgaa aattttgaat
     9121 tttcagaata gaataactta aaagtactga caaaattttt taaaggggca atattaatgt
     9181 tctgagataa tcaaatccta ccgaaaatat caaaaatcca atgtgacaca atcggattgt
     9241 gtctcattcg agggaatcac acaatcccac gtggctctag gcagaattga atttttgctt
     9301 ggggcttttt ccttatattg attttgaatg agacttatta tatgttataa tgcaattaag
     9361 tatcattgga gagggcgaag aaaatgaaga cggtagaacc tattcgagac aaaaaaaaga
     9421 tcgatgcgat gaaggcaatc ttggcttcgg gaaaatatgg ccaacgaaat ttggttcttt
     9481 tttcgattgg gatcaacacc gcctatcgca tttctgattt gcgtcaattg aagctggccg
     9541 acgtgctgga aatttcacgc ggtcgcgtgg tggtcaaaga tcgattagcg atgaaggaac
     9601 aaaaaacagc taaacataat tcggtctata tttccaacaa attgcgaaaa acgattttgg
     9661 agtacgttca acaggaattt cctgatcaac tccttcagca agattttgac ggttatttgt
     9721 tccctagtcg aaaagggacg aatgaaccgc tcaaccgcca aagtctgtgg cgaatcattc
     9781 atgatgctgg cacggcaatc ggattgaaag acattggccc acattccatg cgaaaaacgt
     9841 tcggttactt tctctataaa caggggacca aaacagaaat cattcagtcc ttgttgaacc
     9901 attcttctca gcgcgaaaca ttgcggtata ttggtattac acaagaagac aaagatacag
     9961 ctgtaaaaag cttagattta taatataaaa gttaagttta aacatggata gttttaaatt
    10021 agtagattga gtgttgggag gaaagaatga gtacattata tattatagga aacggttttg
    10081 atttaagtca tggtttaaaa actagataca ctgatttttc aaaatgggtt gaaaaaagtc
    10141 atagtgaaat attcgataaa atagacacaa tgataaatga aaataatgag gtgagaagta
    10201 cagctgatga taagataggt tggtttaatt ttgaaagtag tttgcatgag ttaattcaaa
    10261 gtccagtctt tgaagattat gtagaagagt ctaaggatta tctcataggt tatggacatg
    10321 aagattggtc agattctgcc catcatgatt ttcaaaatat ggtttatgat tatgttgttt
    10381 ctctagaaga attacaacaa cttttcaagg aatggatacg caaagaaata cagccgacaa
    10441 tagaacataa aattccaaaa taccaatttt cgtccgatgt actattcttt acattcaatt
    10501 atacagatgt tttggaaatt ctatacaagc aaccgaaaga aaacatatat catattcatg
    10561 gcagttatga cgatataata atcggccact ccgagaattt tgacaacttt ataggagat
//
LOCUS       DAZUKD010000096        10513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000096 DAZUKD010000000
VERSION     DAZUKD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.098"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..606)
                     /locus_tag="ACG8N9_002137"
     CDS             complement(121..606)
                     /locus_tag="ACG8N9_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4897698.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    696..1041
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1086..1727
                     /locus_tag="ACG8N9_002138"
     CDS             1086..1727
                     /locus_tag="ACG8N9_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4897699.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1746..2381
                     /locus_tag="ACG8N9_002139"
     CDS             1746..2381
                     /locus_tag="ACG8N9_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4897700.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2399..3214
                     /locus_tag="ACG8N9_002140"
     CDS             2399..3214
                     /locus_tag="ACG8N9_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4897701.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3533..4969
                     /locus_tag="ACG8N9_002141"
     CDS             3533..4969
                     /locus_tag="ACG8N9_002141"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4897702.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5158..7605
                     /locus_tag="ACG8N9_002142"
     CDS             5158..7605
                     /locus_tag="ACG8N9_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4897703.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7835..8902
                     /gene="rlmN"
                     /locus_tag="ACG8N9_002143"
     CDS             7835..8902
                     /gene="rlmN"
                     /locus_tag="ACG8N9_002143"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4897704.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8892..9098
                     /locus_tag="ACG8N9_002144"
     CDS             8892..9098
                     /locus_tag="ACG8N9_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897705.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9095..10198)
                     /locus_tag="ACG8N9_002145"
     CDS             complement(9095..10198)
                     /locus_tag="ACG8N9_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4897706.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3468 a         1798 c         2279 g         2968 t
ORIGIN      
        1 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
       61 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
      121 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
      181 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
      241 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
      301 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
      361 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
      421 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
      481 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
      541 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
      601 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
      661 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
      721 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
      781 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
      841 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
      901 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
      961 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     1021 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     1081 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     1141 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     1201 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     1261 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     1321 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     1381 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     1441 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     1501 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     1561 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     1621 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     1681 caatcataat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     1741 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     1801 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     1861 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     1921 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     1981 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     2041 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     2101 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     2161 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     2221 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     2281 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     2341 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     2401 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     2461 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     2521 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     2581 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     2641 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     2701 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     2761 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     2821 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     2881 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     2941 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     3001 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     3061 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     3121 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     3181 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     3241 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     3301 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     3361 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     3421 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
     3481 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     3541 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     3601 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     3661 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     3721 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     3781 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     3841 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     3901 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     3961 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     4021 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     4081 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     4141 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     4201 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     4261 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     4321 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     4381 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     4441 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     4501 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     4561 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     4621 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     4681 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     4741 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     4801 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     4861 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     4921 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     4981 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
     5041 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
     5101 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
     5161 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
     5221 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
     5281 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
     5341 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
     5401 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
     5461 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
     5521 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
     5581 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
     5641 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
     5701 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
     5761 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
     5821 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
     5881 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     5941 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     6001 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     6061 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     6121 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     6181 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     6241 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     6301 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     6361 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     6421 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     6481 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     6541 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     6601 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     6661 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     6721 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     6781 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     6841 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     6901 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     6961 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     7021 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     7081 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     7141 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     7201 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     7261 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     7321 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     7381 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     7441 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     7501 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     7561 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     7621 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     7681 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     7741 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     7801 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     7861 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     7921 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     7981 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     8041 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     8101 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     8161 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     8221 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     8281 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     8341 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     8401 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     8461 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     8521 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     8581 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     8641 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     8701 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     8761 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     8821 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     8881 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     8941 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     9001 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     9061 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     9121 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     9181 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     9241 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     9301 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     9361 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     9421 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     9481 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     9541 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     9601 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     9661 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     9721 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     9781 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     9841 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     9901 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     9961 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
    10021 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
    10081 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
    10141 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
    10201 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
    10261 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
    10321 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
    10381 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
    10441 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
    10501 ataattggtt ttt
//
LOCUS       DAZUKD010000097        10474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000097 DAZUKD010000000
VERSION     DAZUKD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.099"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..263)
                     /locus_tag="ACG8N9_002146"
     CDS             complement(63..263)
                     /locus_tag="ACG8N9_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4897707.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(715..1035)
                     /locus_tag="ACG8N9_002147"
     CDS             complement(715..1035)
                     /locus_tag="ACG8N9_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897708.1"
                     /translation="MKKRVLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1080..1223)
                     /locus_tag="ACG8N9_002148"
     CDS             complement(1080..1223)
                     /locus_tag="ACG8N9_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897709.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(1538..2656)
                     /locus_tag="ACG8N9_002149"
     CDS             complement(1538..2656)
                     /locus_tag="ACG8N9_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897710.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2889..3011
                     /locus_tag="ACG8N9_002150"
     CDS             2889..3011
                     /locus_tag="ACG8N9_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897711.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            3127..4434
                     /locus_tag="ACG8N9_002151"
     CDS             3127..4434
                     /locus_tag="ACG8N9_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4897712.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGESRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            4553..5752
                     /locus_tag="ACG8N9_002152"
     CDS             4553..5752
                     /locus_tag="ACG8N9_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4897713.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5779..7047)
                     /locus_tag="ACG8N9_002153"
     CDS             complement(5779..7047)
                     /locus_tag="ACG8N9_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4897714.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(7102..7168)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7210..7857)
                     /locus_tag="ACG8N9_002154"
     CDS             complement(7210..7857)
                     /locus_tag="ACG8N9_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4897715.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(8265..8768)
                     /locus_tag="ACG8N9_002155"
     CDS             complement(8265..8768)
                     /locus_tag="ACG8N9_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4897716.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8891..9655)
                     /locus_tag="ACG8N9_002156"
     CDS             complement(8891..9655)
                     /locus_tag="ACG8N9_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4897717.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMALTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFMVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKENAESLNVGVAAGIVLYHFSL"
     gene            9763..10038
                     /locus_tag="ACG8N9_002157"
     CDS             9763..10038
                     /locus_tag="ACG8N9_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4897718.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEEDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         3124 a         2169 c         1877 g         3304 t
ORIGIN      
        1 aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg tttatttttt agtataggtt
       61 aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa
      121 gttactgctt ggccttcttc taaagttttg aatccgtcgc cttggatagc tgagaaatga
      181 acgaatacat cgtttccgtt ttctgcagtg atgaatccaa atcctttttc tgagttaaac
      241 cattttactg tacctgtttc cataataaaa atcctccttg tgtatcaaca catattttgc
      301 aattacttga atggtgtata tctggaagat gtttatttga aacaagctac cacagattac
      361 cgaacaaaaa ctactcctat agtatatcat accgtttaca aaaaagctag agtttttcga
      421 ctttttttat taaaaaaagt tttctcagcg attacataaa gtgaaaaacc gaactttttt
      481 taaaataaac gaaatattgt tcagttggtg tttttttgct ctgatgttct tctttaaact
      541 aaaaaaatta ttctttctga ctacttatcg gttctgtttc aacggtcgag gctggggaag
      601 attctttagt aggtctgatg ttctcatggt atttctgata gcctatgaca gtgaccagaa
      661 gaacaaggca aaccaatacg atcaacgaga ttagtttttc ttttttgctc atatctatac
      721 ctcataaata acaggatgat gtccatcata tttctgagta tgagtaaaag caaaagctgc
      781 agctgtagaa tgtaagaaat gccagcaact ttcataatca caggtcggac agtaccaaaa
      841 gtaaccaaat cttggctgca aagaccgttc ttctgtttca ggaatcgcag aatcaagatc
      901 tgttgtctgt acataaacat ttttgtacag acgtgtgcct gtaataggag accgctgtgt
      961 ttcttcagca aaaactggct gacttgttaa acttagggat agaaacagca aacaccataa
     1021 cactcttttt ttcataaaga cctccttatc gataagtttt tcccaattaa aatcatatct
     1081 caagaacgcc cgttattcac attttgttca taaatgatgt tttttgtatc ataatcgtca
     1141 aaaagaggtc gtgaccacgt cacaacctct tttttaaatg gtgttccaga agtagcttct
     1201 tcggaaataa gccgaaattt cacaaaaatt tgagaaataa tttttgtcaa tctcttctta
     1261 tttcttgaag ctgaccactt ctgtcacaac ctcttcctta aacggtgttc aaaagcttgc
     1321 atctgcggta ataagcccac caaacaaaaa atatgaagag ctatttttgc ttgctgttct
     1381 tattacttga tgcagaacag ctttttcaca acctctcata cacggtgttt aatcagctga
     1441 ccttcggtat tagctcaaaa aacggcaaaa tatgtgaaac tatttttccg tttgtttctc
     1501 taatactcag gtcaaaacac tgttttcaca acctctttta ataacgaaga atcgctgtca
     1561 gactcttttc atctggcgca tctttttgat ccaaaataaa cactttcccg ccattaatga
     1621 tcacatcatc tgctaatgca ttcaacactt gacgccgatc atattctatt tcaggatctt
     1681 caccgaaacc atcagctaag tttgacgtag cgataaatag atgcgacact tttccttctt
     1741 tcgcagctgg aacgatatca actaactgat cgatgaattt cttatctaag agtttattgt
     1801 attcttttga ttcttgagct gcgagttcac ttgacagctt cttcgcccct tcttcgatat
     1861 ctctaaagga tagctgcgca ggagaagcag aaatcgcaac gtctgcacaa taatagggat
     1921 ttttagcaat ttttttgaac atcgtctgat tttctggtaa tgcaaataaa taaatcggcc
     1981 attgttctgg attttcgaaa gaatccttca agtacgtatc aacagcttgg taatagttga
     2041 cccaatcgat ctctacttct tcatcttttg gatttacgcc atggaaagtt actccatctc
     2101 gggctccgcc tcctccatta gaagagaagt tcaaaccgcc acctgtcagt tcagatccca
     2161 atgcagtaac gacatcaacc ggtgcccctt ctggtagaga tacttctttt acttgtttgt
     2221 tgctgacttc atacaatttc atggagtcgc ggttcaatcc caacagataa taggaatagt
     2281 tgaactgtcc atttttgata atggccagta aataaggaag atcacttaca tagtattgat
     2341 cgtctaccgg gatactcaaa cgatggatat aggtttggtc agcagttata atgaccgcta
     2401 cactcgtagt tgcactacgc caaaacgaag gatctcctaa caaattttca attttttctt
     2461 ggaacgggga ccagtcatgt tcggaatatt ttttctcaaa acgtttcttt gcttctttcg
     2521 caaaattttt gaatttgatt tgatcttttt ccacatcttg atgggcgaca tgcgtattca
     2581 gcatgatcgt gacgaatggt cctttagcgc cttctgaagt caattgtgac aataattctt
     2641 tatccttttt tgccataaac gtacccctcc taaaaaaatc attcctttgt ttctatctaa
     2701 agtctaccac aacactattt ttccatacaa taaaacgtat ctaaaaaaat aaaacggttt
     2761 ctacggtttt aaatttagct atttttttaa gttatcttca taatttcctc aattatttag
     2821 ccccagtctt caactattct tcatgaaatg ctgataaact aaaaaaaaga ataaaagagg
     2881 tggatcttat gcatttgatt atgattttca gtttattaag tattatcagt ttatttgctt
     2941 tcagcgcttc ccgcttatta gatgcggaaa cacagcctat tcctgtaaaa gtacgtaaaa
     3001 acgaccgcta accttccata acgtgcgttc ttatcctaaa gttttttcag aaacattgaa
     3061 actctctccc ctctctgata aaattttaat ttgtagcgaa acaaattaat tacaaggaga
     3121 tcggatatgt ccataaaaaa aacacgtcta gtcctattat tggctagtct cattctttgc
     3181 cttgcttctt ttctaccagc tgttgctgtg caagctgaag atacgttcaa agtaaatgca
     3241 aaagcagctt ttgctgtaga tgcgcaaagc ggtaagattc tttatgatca agatggagaa
     3301 aaaaccatgg ggatcgcttc aatcacaaaa atcatcggtc tttacatcgt tcttgatcaa
     3361 gtaaaagaag gaaaactatc atgggatgat aaagtttcga tttcagatta tgcagaaaat
     3421 ttgagtatca ccccagattt atcgaatgtt cctttgcata aagaaaacac ctacactgtc
     3481 aaagaattat tcgactctgc aatcatccag tcagccaatg cttcaatggt ggccttggca
     3541 gaaaaaatct ctggcagcga agctaaattc actgaacgaa tgaaagaaca attgaaagat
     3601 tggggaatca aagatgcaac gatcgttaat gcatcaggac tgaataactc ttatcttggc
     3661 gaaagccgac cagaaggtac tggagaaaac gacgaaaatc aaatgtctgc tcaagatgtg
     3721 gccatcgtgg cccgtcattt gatcttggat tttccagaaa tactggacgt atctagcact
     3781 acgacacaaa tgtttggcga aaacacgcaa tcaccagtgg aaatggtcaa ctggaactgg
     3841 atgctcccag gattcgttaa ttacaaggaa ggcgtggacg gattaaaaac tggtacgact
     3901 gagcttgcag gtgcttgttt cgttggtacg atcactaaaa acgatcaacg gatcatcact
     3961 gtcgtattga atgcggacgg ccatgcggaa aacccgagtg cccgcttcaa tgaaacagac
     4021 cgtttgatgg actactgcta tgacaattgg tcagaaaaag agctcggaaa agctaacgca
     4081 agtattccta gcctaaaaac cattgatgta aaagacggaa aagaatcaac agttcctgtt
     4141 gtgcttaaaa gccctgtaaa agtatgggta cgaaacgata tggatactgg caaattaacg
     4201 atcacgccaa cacttgacaa gaagcaagtc actgacaacg agatccaagc acctatggat
     4261 aaaggtacaa aaatcggtac cgctacgatc accttggcag acgataaatt aggctattta
     4321 gaagacaaca atgcgccatc aacagagatc attacggata aaacagtgga aaaagcgaat
     4381 atgttcgtga ttggctggcg acgcgtcgca gaatttttcg gcaacttgtt ttaattgaac
     4441 aataagaaaa gcatctttcc atcggaaaga tgtttttttg ctttttctag tttaaataac
     4501 agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat ttatgaatct
     4561 aaccagagat ctgatctttt tagttctcta tcttattggg gtattctcta tcgtttattg
     4621 gcgaaaacag aagaaggatc cttccttgtt cttttacggc agtacgtgga catttacggt
     4681 aatcttaact tattttgtcg tgttggaaat tttttattcg accttttatt ttttgattcc
     4741 attgttgttc tttagtatct ttgctttttc ttatttcaca gaaaaacgaa ggctgctgaa
     4801 tggtctttta ttcaatgttt tcctcatcag tttcggcatc tacttgtttg tcttgctgta
     4861 tgaaacgcaa aatattttcc ttggcggact gatcgccttg atcacgattc cattgctact
     4921 tgtcctttta tttggtattt atggattgat tgtttttctt ttttggaacg gggtcacagt
     4981 tttacgtaga gaatcgcatt cattagcgaa tcttttaacg ttgatactag ccatttttct
     5041 tacactgttt cttgtttttg actttttctt gttaaaatat ttgccgcaat ggataaatgc
     5101 tttgtttttc tgcgttcccc tgatcttgat ctatctattt atcgtattct acaacttttt
     5161 gactgtctca ttcttgtacc agttcaaccg gcctcgatac aatcaagact tcatcgttgt
     5221 gttaggcgct gggttgatca atggagaaac agtttctcct cttctagcca agcgaatcaa
     5281 taaggcaatt gctttttatc gggctcaaag ccgtgctaca ttaaatccgc ccatcttatt
     5341 gatgtctggc gggcaaggag cagacgaaaa agtaccagag gccattgcga tgaaacaata
     5401 tgccatggaa caaggaatcc ctgaaagaga cattcttgtc gaaaccaatt cgacaaccac
     5461 tttggagaac atgctctatt caaaggaaat catggatcag caaatgaaag ggccataccg
     5521 cgcgatcttt agttccaaca attaccacat ttttagagcc ggtctttatg cgcggcaagc
     5581 caaattgaaa gcaaatggta ttggtgcaaa aacagctttc tactacttgc ctaatgcgtt
     5641 tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa agacatatga tcatttgcgg
     5701 attgcttttc gcaggctccg cattcttatc gctgattact ttacttttct aaaaaaacaa
     5761 ggtgtgacaa aacatatgtc acaccttgtt tttttctttc attctcttaa ctattggcaa
     5821 aaagagtatc ccttttttga ccgctacttg ctgagcagaa taaataccgc tgttgcctga
     5881 acacataaag ctgatcaagc agatcataaa gaagtaaaca gccgcttgtg aaccgaacaa
     5941 ttcgatcccc atcacaaagc aggcaatcgg tgtatttgtc gcaccagaaa atactccgat
     6001 aaagcccaat cctgcgagaa aaggaacaga aacatggagc aaaggcgcta gtgctgcacc
     6061 aagcgttgca ccaatttcaa acaatggtgt cacttctccc ccttgatatc ccgcgcctaa
     6121 agacaaaact gtgaagaata acttcccaat aaaatcatag acatgtgcct gtccattaaa
     6181 tgattcttga agtaacggaa gactcaatcc gagataacgt tgtgtttgca aaatcaatgc
     6241 tgctgccaca actacagccc cacctatcgt atttcgccaa acaggatgag gaatccattg
     6301 tgcataccgt gcttttaaaa agacgatcga acggctgaat acccagccaa tcaaaccaaa
     6361 agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag ggatcacacc
     6421 catttggtaa tgtaggtgag aaacaccata gctttccgtc atgaaatttg caaacagtgc
     6481 agcaaagaag ctagggaaga tcgcttctgt tcggactttc ccgattgcca acacttctaa
     6541 tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc
     6601 actgataatc aagatttccc gttctgcttt atccaatcgg aacaaacgtg ctgtattatc
     6661 tgctaacgct ccccccattt ggactgctgt tccttcacgg ccaacagatc ccccaaataa
     6721 atgggtagtt atggttccaa acagtgtaag tggaataagc cgcaacggga ttttttcttc
     6781 tccaccattt ccttgatcga tcactaagtt gtttccacga ctagaaagtc cgccgtgata
     6841 cgcatagaga taagcaaaaa gcgcaccact aactggtagt agataaagga gccaaggatg
     6901 tgatagtcgg atatctgtca caactgtcaa gctatttaag aaaaaagcag acaagctccc
     6961 catgaatata ccgagaatcg ctgtgatcac cagccacttc agcatgtaca ctaaaatgcg
     7021 tactggactc ggtaattgtt tttccataaa aaattcctcc tcaataaaaa aacgcctact
     7081 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
     7141 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
     7201 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
     7261 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
     7321 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gaaacattcg atgttttctg
     7381 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
     7441 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
     7501 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
     7561 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgaa cgatttgctt
     7621 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
     7681 taaaaatgta tcgatttctg gcagttcgtg atctaatatg tctcgaatct tttcagctgc
     7741 tagtgcctga actgtttgca ccgtttttgg atcattgaca gatgagtacg catttaataa
     7801 ctgttctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
     7861 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
     7921 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
     7981 tcaccccctt taatgcttat tcttctatgt acatgattca ttcgataaat aaaagtaaac
     8041 cgtatcttga acagatagtc aactgcaaac agaaaaaagg actgtgacaa aagtcgtgtc
     8101 aaagtccctt gtttcgaatg aatggtgttc aaaagcttgc atctgcggta ataagccgaa
     8161 acttcacaaa aatttgagaa gtaattttcg taaattcctt cttatttctt gaagctgacc
     8221 acttctgtca caacctcaat atggtctatt attggtttta cctgttaaat cgaggattct
     8281 ttcccaaagt aacgttgcca tctcttacgc atacttgcag acatgggctg tgccgcttct
     8341 ttgactgcgc agtatttgtc cacgaatgtc acgatatatc cttctttata cttaggcggt
     8401 gcgatcgttg caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg
     8461 gtgatttttt ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg
     8521 tcaaatttcg tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct
     8581 gttgctttag cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg
     8641 atggaatgct ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact
     8701 gcttctgttt ctaaaagatc ttgtacataa gacatatatt cttcgtcttc acgccatttt
     8761 tctgtcataa ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta
     8821 agtataccac cagtgttcgg attattctag tcgctctaga taaaatagct aaagcttaat
     8881 agttccttaa tcataaagaa aaatgataaa gaacgatccc tgctgcgact ccgacattca
     8941 atgattccgc attttctttc attgggatat atacgttctg atcagtcaaa gacaagattt
     9001 cctgacttac tccctgacct tcgtttccca tgattatagc gaaatcctct gttttttcta
     9061 ctttgtctaa tgcatgtgct tcttcattta attctgtacc gtacaccata aaccctttgc
     9121 ctttcattac agggatcact tctgccaaat ctttttgtaa cacaggcaaa tgataattgc
     9181 ttccctgcat gcttcgcagc acctttgtac tataaatatc tgccgttcct ttacctaata
     9241 aaacaccagc cataccaaac gcatctgccg tacggatcat cgttcccaca tttccaggat
     9301 cctgtacatt atctagtaaa agccagcctc ctgaaaaatc tggcgtatct tcagatgtta
     9361 acgccatcac tgctaagatc ccttgcggtg taggtagatc tgacatagcg gtcatgacct
     9421 catctgaaac tagaaaagta gaaatctctt tgtttacagc cagccagtct ccccattctt
     9481 tcatacctct ctgtgtcaca aaaagttctt ttatttctgc atcagacgac gcagcttctt
     9541 cgatcagatg gaacccttcc aaaagatatt ctgccttttc ttctctgtat ttctttttat
     9601 gtagtttttt taattctttg attcttggat tttttgctga ttggatctct ttcacattcc
     9661 ttcacctcac ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat
     9721 cggtatcatt gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg
     9781 aatgtccaag gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat
     9841 cagttaggcg tgaccggaac ggtaaaaaac gaagaggacg gctcagtcac aatcgaagca
     9901 atcggaaatg acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca
     9961 agtggacgag tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa
    10021 tttgatgtgg tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt
    10081 ttgtgaaaat gtctttccac atttttgcat aaatttgact taataccgag aaactgactt
    10141 ttgaacaccg tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca
    10201 ttttcaaaat agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat
    10261 aaaacaccgt caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg
    10321 tgaaaaatag ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt
    10381 tgaacaccat cgataaggtt gtttttgagg cactttgcct caagtattaa agacaggtag
    10441 taaaaaagct gatctataac ttgcaatgaa tttg
//
LOCUS       DAZUKD010000098        10274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000098 DAZUKD010000000
VERSION     DAZUKD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.100"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..1238
                     /locus_tag="ACG8N9_002158"
     CDS             183..1238
                     /locus_tag="ACG8N9_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897719.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            1258..1611
                     /locus_tag="ACG8N9_002159"
     CDS             1258..1611
                     /locus_tag="ACG8N9_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897720.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1793..2080)
                     /locus_tag="ACG8N9_002160"
     CDS             complement(1793..2080)
                     /locus_tag="ACG8N9_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897721.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2093..2911)
                     /locus_tag="ACG8N9_002161"
     CDS             complement(2093..2911)
                     /locus_tag="ACG8N9_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4897722.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3149..3370
                     /gene="relB"
                     /locus_tag="ACG8N9_002162"
     CDS             3149..3370
                     /gene="relB"
                     /locus_tag="ACG8N9_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4897723.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            3372..3638
                     /locus_tag="ACG8N9_002163"
     CDS             3372..3638
                     /locus_tag="ACG8N9_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4897724.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            3805..4290
                     /locus_tag="ACG8N9_002164"
     CDS             3805..4290
                     /locus_tag="ACG8N9_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897725.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            4781..5065
                     /locus_tag="ACG8N9_002165"
     CDS             4781..5065
                     /locus_tag="ACG8N9_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897726.1"
                     /translation="MLKATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            5072..5317
                     /locus_tag="ACG8N9_002166"
     CDS             5072..5317
                     /locus_tag="ACG8N9_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897727.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            5339..5593
                     /locus_tag="ACG8N9_002167"
     CDS             5339..5593
                     /locus_tag="ACG8N9_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897728.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            5675..5860
                     /locus_tag="ACG8N9_002168"
     CDS             5675..5860
                     /locus_tag="ACG8N9_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4897729.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            5927..6322
                     /locus_tag="ACG8N9_002169"
     CDS             5927..6322
                     /locus_tag="ACG8N9_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4897730.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            6339..6689
                     /locus_tag="ACG8N9_002170"
     CDS             6339..6689
                     /locus_tag="ACG8N9_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897731.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            6711..7085
                     /locus_tag="ACG8N9_002171"
     CDS             6711..7085
                     /locus_tag="ACG8N9_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897732.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTFITLEENLVISDAGIERN
                     IKRVSIKITLNKPTDKKLSEAF"
     gene            complement(7393..7791)
                     /locus_tag="ACG8N9_002172"
     CDS             complement(7393..7791)
                     /locus_tag="ACG8N9_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4897733.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(7983..8750)
                     /locus_tag="ACG8N9_002173"
     CDS             complement(7983..8750)
                     /locus_tag="ACG8N9_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4897734.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(8786..9946)
                     /locus_tag="ACG8N9_002174"
     CDS             complement(8786..9946)
                     /locus_tag="ACG8N9_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4897735.1"
                     /translation="MKITEKNVNPKFEPTESNQKMFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKTNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYTFDQLV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIAELRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTDIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKFMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
BASE COUNT         3505 a         1759 c         1635 g         3375 t
ORIGIN      
        1 taatatcata ataagataaa tttacaagta tttgtttaca cctttttatt tacactttat
       61 tataattaat tatactttta tgattgcatt tttttcttat aaacagttgc aactaatagt
      121 aaataggtgt aaactatttt cgtaccgaga taccataaac acaaacagaa agaggtcata
      181 ccatgaaaga accattattg ttttccgttg acttagggaa taaatcagta aaagaaatga
      241 gtacccacgt ttctccactc tcacttccct ctcgtatttt gaacaaaaat atcattaatt
      301 caggacgttt ttctaatata ggttcctcat taaaacgatc actaaacgtt tctgaatata
      361 gcctagtaaa cactactgaa agctattact ttgggaagga tatacacgag ctaggaaaag
      421 atgactacgt gatagaatca ctaggttttg ggattagcag atacaagcag gaaacctacc
      481 aaaatatcct atctttctct ttagctaacc tagcacgttt agaagatcaa tctgatgacg
      541 ttttaaccgt taatctagtt ttaggtctgc cttcatccga ctataccgaa acaatcgtca
      601 aatacgtgat gcaacaagcc acacgacaac attcagttga agttgacggt aaggcttata
      661 atataaacgt agataccgtt tgggtactgc cacagccaat cggaacgcta tataacctcc
      721 tacttgctga tgatggtagc gtacaagatg aaaccttgat cagtcagaag atcggttttg
      781 ttgatattgg tggtggcacg aaactttttg atgttgtcca aaattttgaa attgacgaaa
      841 acaatcgtga acaaaaggat accggcattt ttactttata taatcgtatc gtaaatatgc
      901 ttgaccttcc gatcagtcct aatgctttcc aacttgaaaa gattatccga gaaggcaaag
      961 aaaaaggcgt atatctattt aagccaagcg gaaatgaaag ttttgatatt tcagaagtag
     1021 ttgaaagaga aataaaaaca tacacaaata tggttgttgc agaagtttta gctacattca
     1081 aaaacttcac gaccttagat aaagttgtct ttactggtgg tggagcttct attatcgaca
     1141 aacaacttgt aaaagataaa attccgcata ccttgtttgt tgaagacagt gaattttcta
     1201 atgtaaaagg cttttacaag tttggtttaa ctcaataaaa cagcgaagga gatcaacatg
     1261 aataagggaa aatcagagcg ttatgtagtt ataatcaata gagaaaaggt tagcgagtta
     1321 cctttagcag agtttcttga atccgaatat gaaacactag gcgttaaata ctctcagacg
     1381 atcaaaaaag ctttgaacct atatcggcgt gtaaagaaca atgaagttac aattattgaa
     1441 gcaggcaacg atagaaacga tagcaaagta gcagaagtaa agaataaacc aactgatcat
     1501 aaccctattc aacaagagca tccttcaacg gttacatccg atcattcagc aaagaaagaa
     1561 aagccaatgc taaaagggtt caatgcacca agtaacaaag acttacttta aaatgaaaaa
     1621 ggcgaggtta caatacctag cctttttatt atgtacaaat ataaacataa acatatacgt
     1681 atacatgtat ataaacatgt atacgtatat atccataaac acgtatatga aatttagtta
     1741 agctatacct aaccgcttat tccaataaaa aaagtgatca gctttaaact gatcactcac
     1801 caaaaacttc atcatacaag ttatctatca tttcaccaag taaagctgaa tctgatcgaa
     1861 ttcctaactt ttcgctgatt ttagatagct tctttctttg cgatggttga agcgtgaatt
     1921 gataattttt cttcttttca gaactatcac tttttagttt agctaccaaa gaacttgttg
     1981 tttctttagg tgtttgttta ggttcttcaa ctgcttcaac cacttcagga acttcttcta
     2041 cttcagcttc agcgaatttt ttaaatctat tttctttcac ttcaatcact ccttattctt
     2101 gtgccgtatc aattatcttg ttgtatagcg taaacgtgct ttcaaagaaa tccttattct
     2161 tacgatagat attcttatcc ttagcgaact cagcaatatt cttaccttct aagctagttg
     2221 cattaagcaa ttccttctct tgtacatatc caagataatt agataacgtt ggtaccaatt
     2281 ctaggaactc acgacttgaa ttagtattat gtttgatcct gctaccgatc gtagcgtatt
     2341 tagtttccat cagtgtttca ccagtcttaa tatcaatcaa ctgttttgct agtgcatcaa
     2401 tatctttacc aagcttcagc aaagcttcaa aaccatcttt agatggatca gaaatgccta
     2461 cgacaagatc agatacagcc caagcatttt gagctagtag acctgcatca ttatgagtat
     2521 caatcagaat tagatcatac ttctctttaa gctgttcata gttaacaacg atccatctaa
     2581 acagtagggt tctgttgtta ggcatatcac gcatttgcat ctcaacttgt tttaaatctt
     2641 cacctgctac taaaatatcc atgttctcat ttatagctaa aggttcagct gatccatgaa
     2701 gatataaatg attgattgta ttctctcttt tcgctttact gaaagcatca ggaaagcgtt
     2761 ttgataggtt ttgcgactta tcttcatcaa tatacagcac cttcatacct aaattttcga
     2821 cagcatatcc acctagattg tagattgttg ttgttttacc tgttccacct ttttcaatgt
     2881 ttccagtaat gattgacaac ttcttttcca tgattaaacc tcccttatta accttttgta
     2941 actccattat acactataaa cacctttatg tatatgttta tatacataaa ggtgtttata
     3001 tttatatttg cttttatatt ctataacata aacatatata tgtatatgtt tatgtatatg
     3061 tttatgttta tgtatatgtt catatatata actattgcat ctgtcataca atcgtagtac
     3121 aatgtaattg aaaaggagtt gataaactat gccagtaata agtattcgtg tatctaaaga
     3181 agaaaaagat tttcttgaaa aagttgctga attcaacgga gataacattt ctgaatttat
     3241 gcgtagtaat tccctgcaaa cagcagaaac gttagtagac tttgctacct acaaggaact
     3301 aatgaaagag catgaggaaa acgatcaaag tatttcccac gaagatatgc taaaagagtt
     3361 aggcttataa gatgagttac caagtaagat atgaacgtga agctcaaaaa acgctaaaaa
     3421 aaatggataa gtttcaggca aaggttatcc taaactggat cgagaaaaat cttgtaggca
     3481 ctgatgatcc aagaaagcat ggtaaaggtc taacagctaa taaatcaggc tactggcgat
     3541 atagggtagg tgcataccgt ataatagctg atattagcga gcatgaagtg acaattctta
     3601 tattgtctat tggtcacaga agtgaagttt ataagtaaca gacaagctga taaggcttgt
     3661 ctgttttttt gttaacccaa caaaatcact gtttacgatc ccactttacc taaaaaagaa
     3721 caatatcctc ttgttattat acacaccagt gtgtataata taagtgtaaa caagctaatt
     3781 aactttcaaa aggagcgatt ttttatggga tggtatggtg cttattcatc agcaaatgaa
     3841 gtaatcgaag aaaagaaagg gtacgccaaa aatggtttgg aaatcgtaga taccaaagta
     3901 acgaagacct atggaattat ccttttcaaa gatgcagaag gaacctacga tattgacagc
     3961 ttcatctttc gagatgaaat gtacaaacca atctacttct taaatgacag tcctgcttac
     4021 tttaagaaaa ttccaaaaaa atggttgaaa ttctttgtcg gtgatcaaga aaaaatggtt
     4081 aagcgacaac tagatagaat taaagaagaa agcaataaac caaaacttga tgacttatta
     4141 gttgcaggta aaacttactc aatctgggat ggatcgtatg tcgctgttta tcagtataaa
     4201 ctcaaacgta gctttatttt ccgactacaa gatggatcac tcactcggtt cactcgacta
     4261 tcagcaggag atgttaagga agtagcctaa aacttaaata cataaacata aacgtgtata
     4321 cgtttatgtt tatgtatatg tatatgaata taaacgtatt aggtacgaac gcttttaaat
     4381 gcttttctgg gctttgattt tagggtaggg ctaaaacatc accatctgat ccaaaacagc
     4441 tcagaaacgc atttaaacgt atctgatacc aatattacag aaggagtgta tcgtatgatt
     4501 aacctatttc ttttagtgac agctagtaca ttattcttta ctggaatatc acttataatt
     4561 tcctatttga tctataagaa agccgaaact tacacaagca aagcaaccgg actagccgtt
     4621 tcatcctttt taactttgct gacttttttt gttactctat cgttttcttt tgtttagcac
     4681 agctgatcca tcagcaaaaa acctctcttt acattataca cactagtgtg tataatgtaa
     4741 ttgtaaacaa gctaattaac ttttaggaag gaatgacaat atgctaaaag ctacattaca
     4801 gcaacctact cacgttgcta ttgcagaaag ctatacaatt aatgcagggg tcgttaaaca
     4861 gctgatcaag gatcgagaat tcaaaaagtt taacagtcgt caagcgtata acagacagtt
     4921 aaagaaagct attctaaagc acaaaaattc cttcatcggg aaattggaaa gtgtggatgt
     4981 taaaactgtt tcaacggcat tttcagttgt tgaagtgact attgacggtc gcttgatttc
     5041 tctatttgct tttggaatta attgataggg agtgtggact ttggaaggta agacttttgc
     5101 agaaatttgg actgaattta ttgaggtacc aactcgcatt gatccgataa cttctgaaaa
     5161 ggtattggat gcagacttct acaatctcaa agcaggtact agccttgaat gcgtttggat
     5221 gtatttagaa gagcatttca aaggcgtaac gctcggtcac gtcttatacc acaatggtgc
     5281 cgagtgggat gcaattggaa atatccattt ttcttaatac aagaaaggag aacacaatat
     5341 gaattatacc gatgaagaac aaatcacttt ggaatacgtt ttcacaaatg aagaagggga
     5401 ggaaattgaa aaagtcttga tcatttcatc aaaagacttg gagaaggctt tggcttatga
     5461 accatacgaa tcaaccgtaa ctgtcattga attcttagat acctatactt ctaatgatac
     5521 ttgggaaatt atgaatattt gcaacgctaa taacattcaa tttgctgaaa aaaccaataa
     5581 atcattttat tagttttgca ataaaacatc ctctttttct attgattcca tacacaacaa
     5641 tgtgtataat agattttgta aagggagggt agctatgcct aagacagcaa gagaaattgt
     5701 aaaaattctc aaatctaacg gcttcaaaaa gactggacaa actggcagtc atgcaaaatt
     5761 ttataattcc aagactaaaa gaacggtcgt tgttccgatc cacaatggtg atgtaccact
     5821 aggaacagaa gctaatatct ggaagcaagc agggctttaa cagccttgcc ttcctatttg
     5881 gtattaggca tactatcctc tcttacacta gaaaaggagc gaggaaatga agacaaaaaa
     5941 tattttttat ccggcaatct tcgagaaaga aggagaagct tataacatcg ttttccctga
     6001 cttaccggaa atatctacct ttggaaatag tcttgaagaa gcatatttaa acgctaagga
     6061 tgctttagga ttagcgctat atagtgtacc agatgcagaa tttcctaaac catcagcagt
     6121 ggaagaatta actttgaaag ccaaccaagt ggtagtaatc atccaactaa acattaaact
     6181 tttccgaaga agcttaaata caaaaacaat tagaaaaaat gttagtgttc cggaatggtt
     6241 agttctttta gggaaggaga aaaacataaa cttctctcaa ctacttcaaa aagcactaga
     6301 agaagagttg cttgaaaagt aaaaaggaga ccgaacctat gaaagctaca cacttaaact
     6361 atttctctcg aacgttaatc aataatctat taaaagatac aactggcatt agcgttagta
     6421 ccaaacactt tgccaatgat ctaattattt ttgaggataa taacttgcga attgagatgt
     6481 ttattgaaca ggcaacccct aactcaatac ctgaattctg ctatacgcac actgaaaaaa
     6541 tgaaacatgc aaaatcagtt aaagatgttg aagcattaag tgaatcacta acgaacgctt
     6601 taaacctcgt taagaaggca aaattgttat ttgaatttca aaccgactac cacaatgagt
     6661 tgcttgctca atctgaaatt ttagattaat tgaaacagga gaagagaaag atgaaacaaa
     6721 cacaaacaga aaccgttaac gatacgcttg ctgatcaatt agcaggttat tataaattat
     6781 ttgagatgag taaagcacag cgcttgatcc actatctttg gaaatacaaa attttgtttc
     6841 ttttttggga agcaacacta acattctcag ttctttttta taagtcagat ttttataatc
     6901 aacacttagt tttagggagt tttttgctta tctacaccat tttgatgctt aatttatata
     6961 ccttcatcac acttgaagaa aacttagtaa ttagcgatgc agggatcgag agaaacatca
     7021 aaagggtatc tatcaagatc acgttaaaca agcctaccga taaaaagctc tcagaagcgt
     7081 tctgagagct tttgacattt gcttgtataa aatttatcct aacacattac aaagcaccta
     7141 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     7201 tgatggaaaa ctagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     7261 taatacatag caatttaaaa gctctcagaa gcgttctgag agcttttaat atattctttg
     7321 ataatttgtc tttcttaaat ccatacgtcc taaaacaagc tgttattgct taatatgatc
     7381 atttgagaga catcaaatca acttttctct caacaaatca ttcaaagctt tcgacagatt
     7441 aatattctct tcagccgcta aaacatttaa atagtgttcc acagtcactg attttcgatc
     7501 gtaattttgc ttgtttcgag ctttagcaac tttcgtatta gcttttacga caaataaatt
     7561 aacattctca gatcgcttga ttcttgcatt taactcttga acaggcgtag gcgatggaat
     7621 ttctttttct tgcaactcta aaagcaccaa gtatccttcc aatacttctt gcgccatctc
     7681 aaaagcatct tctaaagttt caccatgcgt atataagtct ggaaaatctg ggaatttaac
     7741 tgcatattca tttcctttcc attcgaatac tgcacaaaaa aagtatttca tcgcattgca
     7801 cctctctaat taaaaaataa tttttgcttt ttagtttcaa aaaaaagaat cgcctaccaa
     7861 aaaaattgat aggcgatcac aagctaatta acctctaaag gagattaata ccttgctccc
     7921 aaggcactac atatcttagc atacgtctga agcatcttca aacgagtaag atatattttg
     7981 cattaaccta attttttttc tctataaggt ttttcatcaa actcactaga aatattaaac
     8041 tggacagcaa aattgtaatt tctgcctttt ctaactttag caaaatcaat cttcagcatt
     8101 gatttttcat taatttcctc aactgattcc cttaaaacac gtctatcaaa attcgatatt
     8161 gttttatact cattttctgt tttcgtaatc tcacgaattt taactatttc aatcagggga
     8221 ttcttatata tttcagcttt ctcaaaatct cctttatttt tggcgataag atatttttta
     8281 taatacatcg ccaaatattt gtataacacg atgctatatt ttttttgaaa agatgctaag
     8341 tcttcaaaaa caaagaacgg atacttcttt ttgattgaga caattaaggg aattacatct
     8401 tctgaaaaac tgaaagatac actatgctct tctaaatccc aatgaatccg atcaatagca
     8461 gatacagttg tatacgtctt atatttcttg tctagattcg atatttcata aactgcttgt
     8521 ctttgcaaag tgttaactat ggtttttagt tcagtttcaa gattttgatc aacatccatg
     8581 taattaataa tcgctttaac aggtaaatct actacgcctg ttttctttag ctttgtgggc
     8641 gagatataac tgaacagcaa ttgataaagt tcatacgact ttaaatccat tttcatgtca
     8701 attgttgcta agaactcctc gttgtacttt gtcagttcat cttttttcaa aagcttttcc
     8761 ttccttcacg caagtttttc agttgttaat cataaaggtt cacataaggt aaataactcg
     8821 taattgattt ttcgagatat ttaacagtat tttttattgt gtaatcctcc ttatgagtag
     8881 caacgtaaga tagatgattg acaactgatt caaatcctct atctttggat aaacgatcat
     8941 aaagcggata aactttattc tgaagccgac tcataaattt cagatcgcat aagtcggcag
     9001 aactgatcaa aaagtttttg attagtaatt tagtgtattt gctattcata gctttcaagt
     9061 acgattgatt cattttctct gttttatctt ctgcgccttt taaataagca gggtctcttt
     9121 gttcttgctt ataaggtacc ggtactgaca tggtacgtgt aagcaagaac tttatatctg
     9181 tataagtatg acccttcttg ataagctcat agtcaatttt aaaatcagtg tatttattca
     9241 gttgggcaat cggttcttta attacacgtc tttcaaagtc tcgaaaagct ttatattttt
     9301 tctcaatact gaatttcttt cgtaactctg caattgaaaa aacggggttt tttaatctat
     9361 caagtgattt tgtactccat gttaagttat ctctatttgc ttcatacaga ttaaaatctt
     9421 tagtgaataa ttgaaaaaaa ctgatcgcat atttgcttgt caagttaact aattgatcaa
     9481 atgtatattg tgtaaaatga tcctctaagt tcgttaacaa aggcataatt gaactactaa
     9541 atttaataga tactgcatca gaaaaatcat tgaattcagt aatttctagc ggtgagatga
     9601 ctttgtattc agtcttaccg ttagttttag ttctttgaat tttaatgaca gattgctgat
     9661 gcaatttttc aataacttgt tttaatcttg aatgttttga agaagatgaa ctagaagaca
     9721 tcaacgcaaa taaagccttt ttagatactt tgacggtatt attaaaaggc ggatttttag
     9781 ggtcaagatg tcctatagcg taaagataaa ttttttgtgg catagcatcc atttcaacaa
     9841 tgctatctac aagttgattc gataaagtaa cagttaaatc aaacattttt tgattgcttt
     9901 cggtaggttc aaatttcggg tttacattct tttctgttat tttcatacga ttgcctcttc
     9961 tttctaagct tggtacgagc ttaaaaactt ggaactggta cgattccatt gaataagtat
    10021 agacctgttt tcgatacgat acaataagaa atgtatcgta aatttataac ataactcaat
    10081 ctcagtaaaa cgcattttat ttttattaat caaatttcat tgataagaca gcagaaatcg
    10141 ctattttaga acctttacaa aataaaaatc cttttcaaaa aaaatacgta attataaaaa
    10201 ttcacaaaat taaaaaacaa atattttttt agtttttaga aataaattca catactttgt
    10261 ctttttttgt atat
//
LOCUS       DAZUKD010000099        10269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000099 DAZUKD010000000
VERSION     DAZUKD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.101"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(559..1599)
                     /locus_tag="ACG8N9_002175"
     CDS             complement(559..1599)
                     /locus_tag="ACG8N9_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4897736.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="ACG8N9_002176"
     CDS             complement(1948..2274)
                     /locus_tag="ACG8N9_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897737.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="ACG8N9_002177"
     CDS             complement(2261..3049)
                     /locus_tag="ACG8N9_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4897738.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="ACG8N9_002178"
     CDS             complement(3417..3707)
                     /locus_tag="ACG8N9_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4897739.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="ACG8N9_002179"
     CDS             complement(3843..4022)
                     /locus_tag="ACG8N9_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897740.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="ACG8N9_002180"
     CDS             complement(4019..4369)
                     /locus_tag="ACG8N9_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897741.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..5687)
                     /locus_tag="ACG8N9_002181"
     CDS             complement(4362..5687)
                     /locus_tag="ACG8N9_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4897742.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6137..6751
                     /locus_tag="ACG8N9_002182"
     CDS             6137..6751
                     /locus_tag="ACG8N9_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4897743.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6916..7920)
                     /locus_tag="ACG8N9_002183"
     CDS             complement(6916..7920)
                     /locus_tag="ACG8N9_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897744.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8379..8636)
                     /locus_tag="ACG8N9_002184"
     CDS             complement(8379..8636)
                     /locus_tag="ACG8N9_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4897745.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8629..8898)
                     /locus_tag="ACG8N9_002185"
     CDS             complement(8629..8898)
                     /locus_tag="ACG8N9_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4897746.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9029..9208)
                     /locus_tag="ACG8N9_002186"
     CDS             complement(9029..9208)
                     /locus_tag="ACG8N9_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897747.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            9306..9542
                     /locus_tag="ACG8N9_002187"
     CDS             9306..9542
                     /locus_tag="ACG8N9_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897748.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(9589..>10269)
                     /locus_tag="ACG8N9_002188"
     CDS             complement(9589..>10269)
                     /locus_tag="ACG8N9_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897749.1"
                     /translation="TEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFIQRVEEQLYL
                     ESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSELVENFVSY
                     TICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLM
                     GMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQ
                     RFERKKRQ"
BASE COUNT         3132 a         1876 c         1472 g         3789 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga cgtgccaatg
     4741 tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat atctgttcga
     4921 tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatcaatcct
     5221 agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg ttgacttcgt
     5281 gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga ttcatcgatc
     5341 gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt tacctgcata
     5401 tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt aggaaatggt
     5461 tgtggtaagt cacgtggtct actcacattt gtaataccat accgcttttt tgcttcagga
     5521 gaagaagcta atatcaatcc tgaaccagta ttatcagctc gactcataac aacaagttct
     5581 gttgttaatg gatctaaatt tctttctata cattcgacac ttgcgtaaaa gcttttcata
     5641 tcgattaaaa agtaatcatt tactggctct tttgaataat ccatcataaa taacactcca
     5701 ttctttcaca ttatacaaac atatgttcga tattgtaaag aagaaatagc taaatacata
     5761 aaaaaagaag cataggtatg ctatcaccct gcttcttctt ttgatttgta tgatcaattt
     5821 attctatcac tttactaatt aatttgaaaa tctaataaaa actctcactc taaaagagtg
     5881 agagactgca aattagagta cggacttttt tggagttccg ttctttaata ttaacaaatg
     5941 aataaaatcc ttacaacatt tgagaataag ttatcagtag acacttacaa aaaattctca
     6001 tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat agacactggt
     6061 ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag
     6121 aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
     6181 acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
     6241 atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga
     6301 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat
     6361 cgcttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat
     6421 catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca
     6481 aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg
     6541 aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga
     6601 cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg
     6661 tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat
     6721 aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat tatcttctca
     6781 tctttaattt cctacatata attactatct ataaaattaa gtcttactca gaacagataa
     6841 agcataaaat tccaacaaac cattttttac ttaactaaat aaagagtctg ttaatataat
     6901 cttcacttat ttacgttaat cccctactgt atttgttggt tccttatcta tattttctac
     6961 aaaatcctta ttatcttgtg atttagcttc gtataattct ttgtatgtcg acacattggt
     7021 tatactaatt cctatagcta gttcttgaga ctctactttt ctctctagct cctttgacaa
     7081 agtgatttga gatttatttg gttccgttgg agtcaattct atagattctt cataaaaatc
     7141 tctatacata aagctatcat taattttata aaaaaaatta aatgttttta gcactgccat
     7201 ataacattca aaacattctt ctatagttgg tttacttcct tgaggagaat gaaccaattt
     7261 attacgtttt tctatgaaat ttttaatttc ctttcttatt tctttcggaa actcaatatt
     7321 aaagtaccct ttaaacatgc tccctaataa tttgtctaca gggggagatt gtaaattctc
     7381 aaatattaga gttaaatcag gtttctcttg ttgataaaat tcctttaccc ctatttcaca
     7441 ggctgtaatt atcgaaataa acctatctct agtatttgat atatttttac ttcttttaag
     7501 gagtataaga gaaaagggga tactttcgtg tccatgcatg taattaacta attgttgagc
     7561 ttcagaagtt atatctctaa ttatatagtt aacggaatta ggaagtgtct ttaactctaa
     7621 tgaaatttct atccaattac tatcttctaa tctgtatatg ctcgaagtat aatttgattc
     7681 gtcagcaagc atcgctacat cccaaacact gatcataaaa tcttttaaac gttcaatcat
     7741 ttcagcaata ttgctaaaat cagctaattt ttctttagaa taaaaaaaaa catttatatc
     7801 agaatcaata aattctattt tttttattac tatatcgtca tcattatctt gaccatttat
     7861 gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt tatattccat
     7921 tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata tgtaaataaa
     7981 tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg ctaattaact
     8041 aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc tattcagtca
     8101 tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta ttataacata
     8161 tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc ctcaacttta
     8221 gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc attaataaca
     8281 atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata aggatcaaaa
     8341 acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc ttttgcagaa
     8401 taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc tgtaattctt
     8461 ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa tccagcaaag
     8521 ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct tttgtttcct
     8581 tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt aatcatcaga
     8641 ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt catctcctcg
     8701 acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt gcatagaatc
     8761 ataatctctt tttgataata caacaactgt atcttctaca tctttacttg ttacaataag
     8821 tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc ggaaatttga
     8881 ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca attgtacatt
     8941 atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa agtgctatca
     9001 cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc tcaaatcctg
     9061 ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat tcacttttgc
     9121 cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac gtaacgggta
     9181 accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga aataacccta
     9241 tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat acaaagcgcc
     9301 aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa tgcacagctg
     9361 cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga atgattttat
     9421 tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat ttcgcaccac
     9481 ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttagttact
     9541 aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt attgcctttt
     9601 tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc
     9661 tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc
     9721 tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata
     9781 atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag taaatacaag
     9841 gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt cttccattaa
     9901 ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt tcctgcaaat
     9961 cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat tcatgatgta
    10021 tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag gacgacgttt
    10081 atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt cactttccaa
    10141 atacaattgt tcttcgactc tttgaataaa gtacttcatt tcttccatac aactaaagtc
    10201 tttttgaatc cgtctgatta cttcattcaa atgttctttg tttgttaaaa agtgtgacac
    10261 atcttccgt
//
LOCUS       DAZUKD010000100        10174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000100 DAZUKD010000000
VERSION     DAZUKD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.102"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..72)
                     /locus_tag="ACG8N9_002189"
     CDS             complement(<1..72)
                     /locus_tag="ACG8N9_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4897750.1"
                     /translation="MTFFFFYSIIDKSIVDESIIRKEE"
     gene            complement(484..1752)
                     /locus_tag="ACG8N9_002190"
     CDS             complement(484..1752)
                     /locus_tag="ACG8N9_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4897751.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1983..2720)
                     /locus_tag="ACG8N9_002191"
     CDS             complement(1983..2720)
                     /locus_tag="ACG8N9_002191"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4897752.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2787..3356)
                     /locus_tag="ACG8N9_002192"
     CDS             complement(2787..3356)
                     /locus_tag="ACG8N9_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4897753.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3484..4356
                     /locus_tag="ACG8N9_002193"
     CDS             3484..4356
                     /locus_tag="ACG8N9_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4897754.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4698..5570
                     /locus_tag="ACG8N9_002194"
     CDS             4698..5570
                     /locus_tag="ACG8N9_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4897755.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5567..7525
                     /gene="nagE"
                     /locus_tag="ACG8N9_002195"
     CDS             5567..7525
                     /gene="nagE"
                     /locus_tag="ACG8N9_002195"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4897756.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7857..8708)
                     /locus_tag="ACG8N9_002196"
     CDS             complement(7857..8708)
                     /locus_tag="ACG8N9_002196"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4897757.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8831..9298
                     /locus_tag="ACG8N9_002197"
     CDS             8831..9298
                     /locus_tag="ACG8N9_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4897758.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9561..10043)
                     /locus_tag="ACG8N9_002198"
     CDS             complement(9561..10043)
                     /locus_tag="ACG8N9_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4897759.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
BASE COUNT         3009 a         2062 c         1890 g         3213 t
ORIGIN      
        1 ttcctccttt ctaataattg actcgtcaac gattgacttg tcaataatag aataaaaaaa
       61 gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga aaagtataga
      121 gaaaagaaat tttttgtaga atgaacggaa aaaatgataa agaaagcaca acgctaaaac
      181 caagattatt gattcttatc aaactataga agatagctct tctgcagtag ataattttac
      241 accgccaaaa atttccgaaa tcgccttttg caagaatccc gtcgaatatt agaaattgga
      301 tcagtatgct taaacagtaa taaaaaagtt atgacaagat gttgtcacaa ccttttttga
      361 gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca aaaaacatga
      421 aaatctgttt ttgtttggct gttcttacta cttgatgcag ggcagctttt tcactacctc
      481 tttttaataa ccataaaatc ctattccttc atatctccag cccatcggtg ttagccaatc
      541 tctttcctta gcactcaagg tatagtgatg ggtaccagtt ttttcatttg gattaaatag
      601 tcgataaatt ggcttggaac caccagaata aaaacttaga ccttcataac gccaaccgat
      661 ttttgttaaa gagttgatct cagattgtgc cattgtgtaa tgatggtctc cactgtaagg
      721 attgtataaa cggtagacag gttgtccgct gtttggtgct tgccaagcaa tcccttcata
      781 ttcccagcct acagttggca gataattttt ttctccggca ttcaatgtat ataaatgttc
      841 tcctgtatga cggttataca gacgatacat cggagaagtt gaagttggtg ctgtatcata
      901 ctgtgtcaga ttataggttt cgattacatt gttcaatgta gaaccataat taggcgcagt
      961 ggcataacga cctgttagcc aagccgtagc atctttgtaa gagttcgtat tgcttttcca
     1021 tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt
     1081 atcttgcaat gattctttgt acgaaggata ttttctaaaa gcggcattca ccgtaatcca
     1141 ttgcccattc acatattccg aagtaggcat cgtaacagat tggccattat agcttccttt
     1201 aatcccaaat agattgtaat taggggcaga agccaaagta ctgttccccc agccactttc
     1261 aacgatggct tgagcaatca tgactgatgc atataagtca ttggcacttg ccaaactttg
     1321 tgcagttgag gcaatactat caatgaacgc ttgttgagaa ttagcagatg agcgagcata
     1381 aaaagcatag ccagtatagt tttcgaattc agacaaatct gcttgatggg tatgtgtcaa
     1441 tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca ttctctggat tgttctgagc
     1501 ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc
     1561 ggttgaaatt gtattttctg agacagtagc tgactgcaaa gtactttctg tatcgttttc
     1621 aattcctgtt tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg attcttcagc
     1681 tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa ataataaatt
     1741 ctttgctttc atcttttttc tcctttgaat tatgaaactt ttcctaaata acagtgtaac
     1801 taaaaaagta aaagcaagtc aatgtccata tttaaataac atttgtttct ttttaataaa
     1861 tttgcttttt ctgcataaaa agacgtaaat gaagaatatt cttctccatt cacgccttaa
     1921 attgatggtc gtttgaataa ctatttaaca agtaattctc tctggatatt atccgttatg
     1981 gattatactt cctgtgtcgt cggacggata atcatgttgc tgacagacat ccggtctggc
     2041 gtttctatcg caaatgctac agcagaagct atatcttcag atgttaatcc ccattctttt
     2101 tgcgcttcat gaagttcaag agcagccttt ttgtcgctga tggtttgata taattcagtt
     2161 tgaacagctc ctggagagat gatcgtagat ttgatgttat tttgccgttg ctcttgacgt
     2221 aatccttcca tgattgcacg gacagcaaat tttgttccgc aataaacggc ggaatcagga
     2281 taaacaacat gtcccgccac agaatcagtt gaaatgatat gcccagattt ttgtttttcc
     2341 atgattggaa gtacggcgga aattccattt aacacaccca tgatgttgat atcaagcata
     2401 ttttgccatt catcacggtg accttcaact aatggagcgg ttggcatgat tcctgcatta
     2461 ttgtacaata catcgattcg accgaacttt tccatcgcta aatcgataac tgcttggact
     2521 tgtgcaaaat ctcttacatc tgctgtttga atatatagct cagcttctgg taaagattct
     2581 tttattgctt ttaggcgatc ttctcggcgt gcagctataa ctagtttagc ccctttttct
     2641 gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat gacaatcact
     2701 ttttctgata aatcattcat tctattctcc tcctgttttg aaaaatctct tgcttatact
     2761 cactatctct tatttcatct tactgttcaa ataatccgtt cgacaggttt gacgattttg
     2821 gcagggattc ctgcaacgat ggtattgtct gggacatctt tcgtaacagt tgcattcgct
     2881 gcaacaatgg cattctcgcc aatcgttacg cctggcagaa tagttgctcc tgctccgatc
     2941 caagcatttt tcttgatgtg gattggcttt actgacaagc ctcttcgttc ttttggatcg
     3001 gttagatggt tcaccgtgat gaggttgaca cgaggaccaa tcaatactga atcttctaaa
     3061 cagatgccac ctaagtcaac gaacatcact tgacgattga taaagacatt tttccctata
     3121 ctaatatgtt tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt atcatctact
     3181 ttgtcactaa tgatattttc tagccgtttg cggatatctg ctggttcatg gtacccagta
     3241 ttcagcgctg ctaaaggctc ttcgttttcc gcttggatct gatggatcac ttcaaagagc
     3301 ggatccgtta ctcgaatctc tttattgacg atttgttttt cgatgaggct tgtcattttg
     3361 ctccctcctt ttatccttac tttataacgg acggcttatg atgtctaata cttataaaac
     3421 atacttaact attcctttta actatactta gtggtatgct tttttaaaaa caggagggaa
     3481 aaaatggata ttcgtatact gacgtatttt ttagcagtag ctagagaaaa aaatatcagc
     3541 cgagcagcaa aatctctgca tatcacgcaa cctactctct caaaacaact gaaagattta
     3601 gaagaagaac ttggtgtgac acttttcact cgaggctccc gagagataca gttgacggat
     3661 tctggtcgat atctttattc aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg
     3721 aacaatttaa caaatgacgc aatcatctct ggagaaatct acattggcgg aggcgaaact
     3781 aaagcaatct ctttcatttc tgatagcctt cattcgttga tcaaggcgca tcctgatatc
     3841 cagtttcatt tatatagcgg gaatgcagat gatatcatag acaaactgga tagcggatta
     3901 cttgattttg gactcgtaat cgaaccgaca aacaaacaaa aataccagtc atttcgactg
     3961 ccagcccaag atcgttgggg acttttgata catcggaatc atcctttagc aaagcaaaca
     4021 gtcatccatc cgcaggaact gaaaactgtt cctttgatca tttcccggca aacgtcagtg
     4081 gacagtcaac tagctgaatg gctaggtttc agcatagatc agttatcgat cgtcggaaca
     4141 tataacttat tgtacaatgc ttctttgatg gtcgataacg ggacttttgg tgcattagct
     4201 ctagatggga tcatcaatac ttatgggacg gaaaaagtat tcatcccact cgatccgcca
     4261 ttacaagctt cactgaatct catttggaaa aaagaccagc ctctttctcc tgctgctcgt
     4321 aaatttttag atgtcctaac ggagaaatac agttaagtag agtttttttc cttcgctttc
     4381 agctttttaa caacgaataa gttaagcaaa agatccaatg attaaataac ataagtttct
     4441 taatttgttc cttcctttta tttctgataa ttccagtaaa ttactataat tataaaaaaa
     4501 gaattgacaa aacataactg tctatataaa attgattacg ttaacaataa ttcgtttcat
     4561 gtgactagat catactaggc atgaaaaatc tctgaggaac atggtttccc taggaggttt
     4621 ttcgtgtcta tttttgtttg gccgcaatca actggtctag caaacgtgaa aaccaaatct
     4681 tcaaaaaggg tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca gtattggtag
     4741 atgacggtgg acaggaaaaa gtagctattg gaaaagggat tggatttgac agaaaaagaa
     4801 atgatttgat ttttgatcgt gatgtcgaac ggatatttgt catggaacca gaaggtcaaa
     4861 tcaagttgca aaatttactt aatcagattg atgaacgctt tttatttgca gcagaaacga
     4921 tcattgatca tgcggaaact gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt
     4981 tgaccgacca cattgctttt tctgcggaga acattaataa cggtatcgtc atccgcaaca
     5041 aacttttaag agaaatcgaa gtgctttaca gtgatgaatt cagtattgct caatgggcag
     5101 tagagtatct gacaaaagag cttgggattc catatactta tgacgaagca ggatatatcg
     5161 caatccatat tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag
     5221 tgacgattgt atctgacatc atccatttag tggaatcaga attggaaaca gatatccatt
     5281 cagaaccatt ttcactgaat tattcaagac tggtgaatca tttacgactg cttctacaac
     5341 gaacccatgc gcaacaatat gctgttttag acacggaaat cgtagaaatg gtcaaacgaa
     5401 aatacccaga aagctataag atttctaaaa aaatccgtgt gttgctgacc aaagaatacc
     5461 agctcgcgat cacgaaagaa gaactgggtt acttggccat ccatatcgag cgtttaagaa
     5521 gcgcaatcgt acaaacaaat gttaacaatc atgaggagga aaaaaattga aagcatatct
     5581 acaacgaatg ggacgttccc ttcaacttcc agttgcggtc ttacctgctg ctgctttact
     5641 agtagggatc ggaaactggt gggcatctta tagtaacgac attgtcgctc attttttaca
     5701 agctggcggt aacgcagttt taggtcaatt acctttgctg tttgccgttg ggttagcact
     5761 agggatgtca gtggacaaat caggtgctgc tgccttggca ggtttggtcg catttgaagt
     5821 tccagtaaat gttttaaaaa ctgattcagt agctacttta ttgaatatca aaccagaatc
     5881 agttgatcca tcttttgctc agatcagtaa cgtatttatt ggtattattg ccggattgat
     5941 tgcggcagct ctttataatc gtttccacga agcaaaatta ccgatggctt tatctttctt
     6001 tagcggcaag cgtctagtgc caatcttggc tgcctttgtc atgttgctga tcagtgcagt
     6061 gctactgctc gtatggcctc cggtttactc tgctttagtt tcttttgcta aattcatttt
     6121 gagtttaggc tggggtggtg caggtcttta tggcttcttc aaccgtttac tgatccctac
     6181 aggattgcat caagcaatga actccgtgtt ctggtttgac gtagcaggaa tcaatgatat
     6241 tggtaaattt ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt
     6301 tttcccaatc atgatgttcg ggcttccagc aggagcatat gctatttaca gaaatgcacg
     6361 tccagaaaac aaagcaaaaa cagcttcctt gatgttagca gcagcttttg cttcattctt
     6421 tactggtgta acagaaccac ttgaattctc atttatgttc gttgcttggc cattatatgt
     6481 cttgcatgca attttcactg ggatttcttt agcagtgagt gcattcttcc attggacagc
     6541 aggatttgcc tttagtgccg gatttgtcga ttacttccta tctttgaaaa atccgattgc
     6601 gaatcagcca ttgatgttga tcgtccaagg attagtaaca gcagtgattt actactttgg
     6661 ctttaacttt gctatcaaaa aattccatct gatgactcct ggccgcgagg aagcagatct
     6721 ttcagatgac gatactgcaa cgactaattc agacaataaa tatgcggcac aagcaagcaa
     6781 aatctacgct gcattaggcg gagccgacaa tgtaacttcg atcgataact gtactacacg
     6841 ccttcgtttg caagtcaaag atacaggcct gatcgatcaa aataaaatca aagccactgg
     6901 cgtacctggt atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt
     6961 acaattcgtt gcagatgaga tggcaaaact acatggtggt gctgcaacac gacctgcaca
     7021 aacaaacacc gtggttaaaa cagaaacatt tgccggtgga acgatcgaac aagatattta
     7081 cgctatcgca aatgggaaac tgattccaat cactgaagtt tcagatgatg tattcgcgga
     7141 aaaaatgatg ggtgacggct atgctgtatt accagaaaat ggagaaatct tctctccaat
     7201 cgctggtacg atcacaaaca ttttcccaac gaaacatgct gtcggaatcc aaacagatgc
     7261 tggtatcgaa gtcttgcttc atatgggcat caacactgtt gacttaaaag gcgaaccatt
     7321 cactttatat gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg ctttagttga
     7381 tcttgccgct attcaatctg caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg
     7441 cgacaaagtc caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat
     7501 cggctctgtt tccaacaaag cctgagccat cgtcttcccc agactttttt atcgataaac
     7561 aaacaggagc tgagacaaaa atcttgtctc agctcctttt tccgaataaa aacggcgttc
     7621 tcctgccaac tccgcggtaa taagtccagc caaacgtaac atatgaagag ctatttttta
     7681 tttgactctt cttattactt gatgcagaac agctttttta caacctctga taaacggtgt
     7741 tcaaaaacca gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa agccattttc
     7801 acaaaatttt tcttcatact caaaactaat cggctttttc acaacctctt tatacattat
     7861 tcctgatcca atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact
     7921 ttttctaaat catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct
     7981 aaaaacatgt cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct
     8041 gcatcacttg cttcaataat ttctaacttt ttacctgtcg cacgctctaa tgccttggct
     8101 aattcttcat agtcagttag attgccggat aattctaaga tttctggaga gtcactttct
     8161 actagtactt ttgctgcagc ttctgcatat tcacgtttca atgcccaacc tgtttttcca
     8221 tttcctgcag cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca
     8281 ttctctacat accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt
     8341 ttttcagtga attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa
     8401 cttgtataag cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt
     8461 tcggcttgac gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac
     8521 gcttctttca ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct
     8581 ttcagtgctt ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc
     8641 atctttttta agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa
     8701 taatgcatat ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta
     8761 taaattacac ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg
     8821 atgacaactg atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat
     8881 tgaaatgttt caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac
     8941 aaatgcagca aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac
     9001 aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga
     9061 tgtctatcgc agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc
     9121 aaattgcgtc gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca
     9181 gaaaaaggca gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc
     9241 cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt
     9301 ctctctactg tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc
     9361 gggggacaaa agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga
     9421 agtcatgacg tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa
     9481 atgcaaaata tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta
     9541 ctttcacgac ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata
     9601 tatttcccgt tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa
     9661 tcttctactt ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct
     9721 tcaggcgatt ctgctaaagc gattacaaca agatacgttc cttttgggaa tgggatgatt
     9781 cgggtagtgt ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg
     9841 ttatagggtt cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct
     9901 attgttccat taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca
     9961 aaatccgtat tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt
    10021 tcttcgatcc tatactttgc catttctccc accctttcta ttatattaag ttcatcttac
    10081 ataaaattaa agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt
    10141 aactattttt ttgctttcac tgcaatccaa atct
//
LOCUS       DAZUKD010000101        10094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000101 DAZUKD010000000
VERSION     DAZUKD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.103"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG8N9_002199"
     CDS             complement(46..366)
                     /locus_tag="ACG8N9_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4897760.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG8N9_002200"
     CDS             complement(514..2748)
                     /locus_tag="ACG8N9_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4897761.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG8N9_002201"
     CDS             3227..3493
                     /locus_tag="ACG8N9_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4897762.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8N9_002202"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8N9_002202"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4897763.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRGLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG8N9_002203"
     CDS             5384..6430
                     /locus_tag="ACG8N9_002203"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4897764.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="ACG8N9_002204"
     CDS             6447..7670
                     /locus_tag="ACG8N9_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4897765.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="ACG8N9_002205"
     CDS             7759..8646
                     /locus_tag="ACG8N9_002205"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4897766.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="ACG8N9_002206"
     CDS             8732..8962
                     /locus_tag="ACG8N9_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4897767.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="ACG8N9_002207"
     CDS             complement(9019..9984)
                     /locus_tag="ACG8N9_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4897768.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3213 a         1785 c         2039 g         3057 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtggtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aaataatgga caagttttag ggaattctat agttctgttc acagaaaata
    10081 ctttgataat caaa
//
LOCUS       DAZUKD010000102         9955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000102 DAZUKD010000000
VERSION     DAZUKD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.104"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..439)
                     /locus_tag="ACG8N9_002208"
     CDS             complement(<1..439)
                     /locus_tag="ACG8N9_002208"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4897769.1"
                     /translation="MEEFGIDSISRTEALDFQADYEYELDQITMIPEIHQLLLALHKN
                     RIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGAAENSYEGNVKNLQSY
                     QNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRHK"
     gene            complement(662..1429)
                     /locus_tag="ACG8N9_002209"
     CDS             complement(662..1429)
                     /locus_tag="ACG8N9_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4897770.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="ACG8N9_002210"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="ACG8N9_002210"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFS4897771.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="ACG8N9_002211"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="ACG8N9_002211"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFS4897772.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="ACG8N9_002212"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="ACG8N9_002212"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFS4897773.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="ACG8N9_002213"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="ACG8N9_002213"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4897774.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="ACG8N9_002214"
     CDS             4828..5145
                     /locus_tag="ACG8N9_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4897775.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="ACG8N9_002215"
     CDS             5145..6851
                     /locus_tag="ACG8N9_002215"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4897776.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="ACG8N9_002216"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="ACG8N9_002216"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4897777.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="ACG8N9_002217"
     CDS             8422..8694
                     /locus_tag="ACG8N9_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFS4897778.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="ACG8N9_002218"
     CDS             8715..9614
                     /locus_tag="ACG8N9_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4897779.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3138 a         1678 c         1996 g         3143 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcgt
//
LOCUS       DAZUKD010000103         9933 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000103 DAZUKD010000000
VERSION     DAZUKD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.105"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..606)
                     /locus_tag="ACG8N9_002219"
     CDS             complement(94..606)
                     /locus_tag="ACG8N9_002219"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4897780.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(620..2917)
                     /gene="recJ"
                     /locus_tag="ACG8N9_002220"
     CDS             complement(620..2917)
                     /gene="recJ"
                     /locus_tag="ACG8N9_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4897781.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLANEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3059..3502)
                     /locus_tag="ACG8N9_002221"
     CDS             complement(3059..3502)
                     /locus_tag="ACG8N9_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4897782.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3503..4291)
                     /locus_tag="ACG8N9_002222"
     CDS             complement(3503..4291)
                     /locus_tag="ACG8N9_002222"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4897783.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4288..5229)
                     /gene="rnz"
                     /locus_tag="ACG8N9_002223"
     CDS             complement(4288..5229)
                     /gene="rnz"
                     /locus_tag="ACG8N9_002223"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4897784.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5406..5993)
                     /locus_tag="ACG8N9_002224"
     CDS             complement(5406..5993)
                     /locus_tag="ACG8N9_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4897785.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6214..7527)
                     /gene="obgE"
                     /locus_tag="ACG8N9_002225"
     CDS             complement(6214..7527)
                     /gene="obgE"
                     /locus_tag="ACG8N9_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4897786.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7815..8825
                     /gene="gap"
                     /locus_tag="ACG8N9_002226"
     CDS             7815..8825
                     /gene="gap"
                     /locus_tag="ACG8N9_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4897787.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8910..9845)
                     /locus_tag="ACG8N9_002227"
     CDS             complement(8910..9845)
                     /locus_tag="ACG8N9_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4897788.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2732 a         2279 c         1718 g         3204 t
ORIGIN      
        1 accctcaaaa gtcagataga gaatctgact tttgagggtc gctttaaaat cgcttcttta
       61 agaataacat caagaatagc ttcttttttc tgcttaatat tccattaatg taatgatgtc
      121 atatcctttg attttgtcgc gtccatggag atccatcaat tcgatcaaaa atgcgcagcc
      181 tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc gctttgattg ttcctccagt
      241 agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt ttaatggcat ccttatgcaa
      301 tgtcaatgta tctgttccat attctaatcc ataagtcact tcgatggttt ctcgaggtaa
      361 cttccctttt ttgcggactg gggcaaaacc gactcctaac tcataagcaa caggacatcc
      421 tacgatgaat ccacgggctt ctggcccaac taccatatca attcgttttt cttttgcata
      481 atttacgatt tgcttcgtag cttcccgata agcgtcacca tctgccatca gtggggaaat
      541 atctcgaaac acaattcctt ttgacggata gtcgggaata ctggcaatat aatcttttaa
      601 atccactctt tgtttcctcc taggttgtta accatttctt tatggtagga atatcgctca
      661 aaagcaaaaa ttcctcaact ttgattttat tgagccgccg ctggtataat ctgctctcag
      721 tcaaaggatg actttctggg ctagcgactt tttgtaaaac accgttctct attgtaacaa
      781 atttcaattc aaaaaacacc tgtatcataa aaattaataa tttttcaggg atacgcaaat
      841 attgagctat tgcttttatt ttatgacgta tatcaattcg ttcttgagaa tggatcagtt
      901 taaacaattt tgcgtactgt tctcttgtgc ctaccccatt caaataggcc tcatcaggcg
      961 aaatccccat cagataaaca cgattgaatg aataagaccg aaaaacttct ttcagatccg
     1021 ctaaatcatc tggacaatcc ataataacta gagagttcgg ttttcttttt tcaattattg
     1081 cctttacaga atctagtcct tcgaaaacaa tcgcagcttc ctggataatc tcaggcacga
     1141 atttcaacgt agaagaatca aatgctaaat aaaggctacc ttccgacgtt cggctctgtt
     1201 cacggaaacg ttttgcccgc caatcaaata cttgcagacc acccacagaa aaatcagata
     1261 ccatcagctg cggtttctta cgcccgttcc attcattgat cgaaagcttt cctaccacat
     1321 ctacctgatt attcagcagt tcttcttctt gagtaccaaa accaaaagcg accgcatcaa
     1381 gctgactcga tgcattggaa aggacaaatt tcagatgctg ttggtttgct ccgattcttt
     1441 tgatattttc tgcttggact tgtcgaaata aaaactgagg caaaggatta tccgtaccaa
     1501 atggcgccaa tagtttcagc tcatcaattc gctctactgt tacttcattt gccagcaata
     1561 cttcatcaat tctcaactca ggccctctca tcaagtcaat ttgatgatcg actatatatt
     1621 ggttcatctt ctcctgtagt atagtgacat tttcacttgg catcgttaaa ccgactgctg
     1681 catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg gtcaagcatt tcaaaaagat
     1741 tcaaggcttc aatactacgt ccagatcctt tagctgatcc atcttctttt aaagtaagga
     1801 caagcgttgg tttacccgtt tcattcatta ttttcccagc aacaatgccc aaaacgcctt
     1861 catgccatcc tcggtttacc aatagatgga tatgattctc cttattgacc atcgctaatg
     1921 cttcttgtgt tatctgctct acgattgctt ttctttcttc attgatctca ttcaattttt
     1981 ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag taacgagacg gcaggacgtg
     2041 gatcgcccat ccgaccgatt gcatttagtc tgggagcaat cgaaaaaccg atcgttgttt
     2101 cgtctgcgtc cctcattttt acgccgcttt cttcaaatag cgcctgaagt cctatccgtt
     2161 ctgaatgatg aatggcattg attcctaatg aaacaaggat tcgattctca tcagtcaatg
     2221 aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa aaattctaca ggcggttctt
     2281 ccaataatgc agaagctact ttgaatgcta ctccgactcc tgccaaatct ggaaatggat
     2341 agtgccccgc tggatgacga gggtgaacaa tcgcataagc gtctggtaaa ttctcaggta
     2401 gttcgtgatg atctgtaatg atcacgtcta caccagcttg ctgagcgtat gctaccgcat
     2461 catttcctgc taccccgtta tcaacagtga tgatcaactg gattccttct tcgatctttt
     2521 cttggtatac agattggttt ggtccatacc catgctcaaa tcgatttggc aggaatactt
     2581 ctacttctgc accaagtaat tccagtgttt ctttcattac cgttgcactg gtgatcccat
     2641 ctgcgtcata atcaccgtaa acgaggattt tttctccact gattacagct tcttggattc
     2701 ggctgattgc tttatccata tcgtgcatca gatacggatc atgtaattct tgttcctttg
     2761 gatgtaaaaa tcggttgata tccttttcct catggtaacc tctttgccac agtaacttac
     2821 cgacaaatgg agataatttc agttcacgga tcatttggag aaaggattca gaaggctctg
     2881 tctcgcttaa cttccaatca aaatttgctt gtctcacttt ctcactccta acttagttat
     2941 tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga ataaaaacag atcagatccg
     3001 ctagacagat gttcagcagg acctgatctt ttgtacgacc atttatgaaa tatccctatt
     3061 agtcaaaata attgaagctt tgtttgggcg attgagtttt ttgttcttcc ggcgctttca
     3121 attgactgat ttgtgcggtt ttcgcttgta actccgcctg ataagcagct tccaactccg
     3181 cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc ttcttcaatt ttcttttgaa
     3241 gttctgtttg atattctgac atttcctgag tcagctgttt gatttgcttt ttttgctgcc
     3301 atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc aatcagcgca gaaccgatga
     3361 tcactaagat caatggcgca gaaaaggaag tgaagccaaa acttacagga acatttttat
     3421 tgttcaatac agcaaaaaga acaataatca aaactaaaac aaatccaaga atcacattgc
     3481 tttgcttttt caattccttc acctactttt tgttaaaaat tccgctggct aaataatcac
     3541 caatcgttgg aaacagcgta taaaattttg atgcaacttc taaaataaac ggttgattga
     3601 tttcccgttt tggatgtctc atattgcgga cgatcatttt tgccaacttg gcaggttcta
     3661 aaacgatttg tccgactttt tccaaatagc ttccgcttgg atcagcctta tcgaagaaat
     3721 ctgtctcgat tggacctgga ttcactgtcg taacagctac gccaagtggt ttcaattcta
     3781 atcgcaaagc gttggagaag ccaaggactg caaatttcgt ggcagaataa accgttgatt
     3841 ttgcagttgc catttttcca gccatagacg cgatattaat gatatgacct tgtcttcttt
     3901 ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat tcctaagatg ttgacttcaa
     3961 acatatcgta tgcttttcca aggtcgaatg taaggaagtc ttcgaaaatc ccaaaacccg
     4021 cattgtttac cagaatatcg acgcgcccga cttcttccat caccttttca tataattcgt
     4081 ccacgctttc aggattgctg acgtctacct tgaaagcata agcctctttg ccgctcaagc
     4141 gtctgcactc gtctctgaca ccttctacaa atgccattct tcgtgcgcaa cttataacga
     4201 ttgctccttg ctttgctgct tcataacaga tttgtgcgcc cagaccgcct gaactacctg
     4261 ttactaaaac aactttatct tctaaattca tgcctcttcc tcctcgcgaa atggaatctc
     4321 aatgctatca aaatctttca tgatcgtcgt gttttcaaac acttcttgtg cttctttttc
     4381 aagctctaaa gctgcttttc ctaaatatct cgcactgata tgagtaagta gcagccgttt
     4441 tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc gtggaatgga aatagttgtg
     4501 cgccattctt gcttcatctt tattaaatgt gctttcatgt accagaatat cgctattttc
     4561 cgcaagcaca acggaattat gtgtttttct cgtatcgcct aaaatagtca cgattcgtcc
     4621 ttttttatct ggacctacga aatcttttcc attgatcgtc cgcccatctt ctagttggat
     4681 cgtttcacct tgcttcagtt tgccgtacag tggtccagct ggaataccta attcttttag
     4741 acgatccacc tgcaattctc ctttatgatc atgttcaacc acacgataac caaaactgtc
     4801 gatcccatga tttagttttt ttgtataaac agaaaattgc tgatcagtaa aaatcggggc
     4861 tgtttcattt agctcaatga atttcagcgg ataacttaaa cgcgtttgcg aaatacctaa
     4921 agagactttt atgtattcct ctatcccttt tggcccgtaa atctctaatg gcgtatcccc
     4981 accttgaaat gaccggctgc tgatgagccc gggaagtcca aaaatatgat cgccgtgtaa
     5041 atgtgtaata aaaattttac cgatttttct cggacgaata gtcgttcgca aaatctgcat
     5101 ttgcgtgcct tcgccacagt cgaacaacca aacttcattg cgctcgtcta aaagtttcaa
     5161 tgctatactt gtcacatttc tatgttttgc aggaacacct gcacctgttc ctaaaaattg
     5221 taattccata atctacctga ctttctttaa gttcctcttt aattttaggg taaaaagaag
     5281 ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa ataacgttct aaagcgatag
     5341 cgttttcttt gaaaattact ataaaaacag cagcgaaccc gtttgagatc ctctgctgtt
     5401 ttttattaat ttgaagatcc agaactttca ttgtcgttgg aagtttgcgg atgtttgtcc
     5461 ttccaagtct tttgtgcagc ttctttcgca tgattttcag cttctttcgc atcatgcaca
     5521 tctttttcga catcttcata atccaagcgg gtaatttctc cgcctaattc catcattacc
     5581 aattgattca gtgggcgatt gtcctcttca tcagcaatca agatcagtcc tgtagctcct
     5641 tcaccgattt tatttcctac aaagtcgaac acggtctggg catcttttat ttctttggca
     5701 tcttgtgtcg caccgatcat tcctccggca aaccatccta ataaaatccc tagcggacca
     5761 cctaatacac caaccaacat tccgattaat ccacccgtag aagatttgtt attaccggta
     5821 aagtcgataa aatcttctat tttgaattga tgtgtcccat taggatcatg gctgacaact
     5881 gccatctgtt ctcctttaat cgaacgaaca gcatgtaact ttttgatttc tgaaaatgcc
     5941 tgatatgcct ggctttctac ttgaaaattt aaaatgatta ctcgtttgct cattaaaaac
     6001 gcccctttca taccttcttg ttatttattt tacctttaat tgtaaaataa gaaaagaaaa
     6061 gcactctttt tttccaataa aatagggctg tgacaaaagt cgtatcacag ctcctttttc
     6121 cgaataaacg gcgttcttga agctgatcac ttctgtcaca acctccctta aatctgaatc
     6181 tatatttttt ggtatttgtt tttatttgct tgcctattcg acaaattcaa attcaaactc
     6241 accgatacgt acaaggtcgc catctttcgc accgcgtgcc cgaagagctt catccacacc
     6301 cataccacgt aattgacgag caaaacgcat cactgtttca tcatgatcga aattcgtcat
     6361 ttggaataat ttttcgatct tctcaccaga taagatccac gttgcatctg aatcacgatc
     6421 gatttggaac tcaggacctt ctggattgaa tccatagtgt acagtttctt cttccagttc
     6481 ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa tctgctgtag cattcaataa
     6541 cgcgtccaat ccttgtcgag tcactccaga aattggaaat actgggatgt catcagcaaa
     6601 ttcatcttct ttttccttgt tcagctgttc tttgaatttc actagatttt ccggtgcatc
     6661 cggcatatcc attttgtttg ccacgatgat ttgcggacgc tctagcaagc gtaagttata
     6721 agtagataat tctttgttga tggcaagata gtcttcataa ggatctcgtc cttccatccc
     6781 actcatatcg atcacatgta aaattactcg tgttctttcg atatgacgaa ggaattgtgt
     6841 acccagccca acaccttgcg aagctccttc aatcaagcct ggcaagtctg ctgcggcaaa
     6901 acttctgcca tcacttgtag taaccattcc tagattaggg acgagcgtcg tgaaatgata
     6961 agcgccgatt tttggacgag cagatgaaat aacggatagt aaagtcgatt tcccgacaga
     7021 tggaaaacct acaagtccaa cgtctgccaa tactttcaat tctagttcga tttttcgctc
     7081 ttgtcctggt tcgccgtttt cagcaatttc cggtgcagga ttccgaggag aggcaaaacg
     7141 gatattccct cgtccaccgc gtccaccttt tgccacaact agtgtttgac cattttcaat
     7201 taaatctcct aaaagagcac cagtctccgc atctcggaca gtggttcctt gtggtacttt
     7261 aacataggta tcttctgaac cacgaccgtg catcccttta ctcatcccat tttcacctgg
     7321 ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat gtacgtaacc cttcatcgac
     7381 gatcaagatg acatcgccgc cacgaccacc gtctccgcca gccggtccgc cgtcaggaac
     7441 gtatttttcg cgacgaaatg caaccattcc gtctccgccc ttgccagctt tcacatcgat
     7501 cgttacttga tctaaaaaca tggacataat taagtcctcc tattattcat ttatttattt
     7561 gactgcctga atttccacaa taaacaaaag tcttacttat tttatcggtg aaatctgatt
     7621 ttgtctatca tttcagcaaa aagattgttt accttcttct ttttttatca tttggtaagg
     7681 tttccaacct tttttctgct accaagtata attttttgac aaaatattct ttgcgtagca
     7741 gaataacagt gtattaattg tattaattga ggatgccgtt tcacagcacc cctaataaag
     7801 gaggaacttt tgatatgaca gtaaaagtag gtattaacgg atttgggcgt attggccgtc
     7861 tggctttccg ccgcatcaaa gaagtctcag atgatattga agtagtagca atcaacgact
     7921 taacaagtcc aactatgtta gcacaattat tgcaatttga ctctactcat ggtacttatc
     7981 ctggcacagt tactgcaaca gaagactcaa tcgtagtgga tggcgaagca actcgtgttt
     8041 atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga aaacggtgta gatatcgtac
     8101 tagaatgtac tggtttttat acatctgaag aaaaagcaaa agcgcattta gatgctggtg
     8161 tcaaacgagt agttatttct gccccagctg gtgctatgaa aaccatcgtc tataacgtca
     8221 atgatgatac attagatgaa aacgacaaaa tcatctcagc aggttcatgt acaacaaact
     8281 gtttagcacc tatggcttat ttcttaaata atgaatttgg gatcgaagtt ggtacaatga
     8341 caacagttca tgcctacact tccacacaaa tgctcttaga tggacctgta aaaggcggta
     8401 atttgcgtgc tgctcgttca gctgctgaca atactatccc acattctact ggtgctgcga
     8461 aagcaatcgg cttagttatc cctgagctac aaggaaaatt acaaggacac gcacaacgtg
     8521 ttccagtcgt tgatggttca ttaactgaat tggtttcagt attgaaaaca aaagtaactg
     8581 ctgatcaagt aaacgaagca atcaaaaaac acacagttga caatccttca ttcgggtatg
     8641 atgaccgtca aatcgtttct agcgatgtca ttggaacaac tcaaggttcg atctttgacc
     8701 caacgcaaac agaagtaaca actgctggtg atttccaact tgttaaaact gttgcatggt
     8761 atgacaatga gtatggattt acttgccaaa tgattcgctt actagaaaaa ttcgctaact
     8821 tgtaatttga tagatcaatc gataaaagaa gcctttgact ggatccagtc aaaggcttct
     8881 ttcttttcat cccctacttt taaactcgtt caatgaacat attggcttaa aaattgttgg
     8941 actacttgct gatatttttc aggatctgtc ttgtatgctt ccgcatgctc ggctcctttt
     9001 acaacatact tttcttttgg tcccttcgtt gcacgataga ccttatctaa catttcaaat
     9061 ggtacaaatg tatcttcgtc tccatgtata aagagcattg ggcgctggtt tttcttcaat
     9121 tgttccactg catttgcttc accgaaaaag tagccagcac ggacttttgt cactagactc
     9181 gttacttgca taagcggaaa tgctggaaga ctaaacatat cgtctagctg ataagcaagc
     9241 tcatcatgaa cggaagaata accgcaatct tcaatgattg ctttcacatt atctggtagt
     9301 ttttctccgc tagtcatcat aactgttgct gctcccatgc tgacaccgta tagaacgatt
     9361 tcttgcgatt ttccattgtt ttctagcact ttgtttatcc actgtacata gtctttacgc
     9421 tctggccatc caaatccaat gtaatcccct tcgctttttc catgtcctct tgcatcagga
     9481 acaaggacat tgtaacccat atcatgaaac atctttgcgt aattggtcat cgtttcagcg
     9541 tttcccatat agccatgagc cacaagagct gttttttcac ttttcgtttc agcaggaaga
     9601 taaatcgctg ataatttcag accgtctttt gaagtgatgg tccaatcgct tcggttttct
     9661 tttgaactaa accatttatg tgcatccgtt tctttggatg tctgtgttgt accaggtgta
     9721 tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat aattataaaa ataattccct
     9781 gcaaacacta acgcaacaat cagagctaaa gcgacgccta aaaggatttt catccaaagt
     9841 ttcataaatc atccatctca tttctctttt ttcatataac cttgaattag tataatcaaa
     9901 gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DAZUKD010000104         9887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000104 DAZUKD010000000
VERSION     DAZUKD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.106"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..221)
                     /locus_tag="ACG8N9_002228"
     CDS             complement(<1..221)
                     /locus_tag="ACG8N9_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4897789.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEK"
     gene            422..1636
                     /locus_tag="ACG8N9_002229"
                     /pseudo
     CDS             422..1636
                     /locus_tag="ACG8N9_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1815..2144)
                     /locus_tag="ACG8N9_002230"
     CDS             complement(1815..2144)
                     /locus_tag="ACG8N9_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4897790.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(2134..2397)
                     /locus_tag="ACG8N9_002231"
     CDS             complement(2134..2397)
                     /locus_tag="ACG8N9_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4897791.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2857..3660)
                     /locus_tag="ACG8N9_002232"
     CDS             complement(2857..3660)
                     /locus_tag="ACG8N9_002232"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4897792.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3638..4762)
                     /locus_tag="ACG8N9_002233"
     CDS             complement(3638..4762)
                     /locus_tag="ACG8N9_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897793.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4778..5593)
                     /locus_tag="ACG8N9_002234"
     CDS             complement(4778..5593)
                     /locus_tag="ACG8N9_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897794.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5583..6455)
                     /locus_tag="ACG8N9_002235"
     CDS             complement(5583..6455)
                     /locus_tag="ACG8N9_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4897795.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6540..7829)
                     /locus_tag="ACG8N9_002236"
     CDS             complement(6540..7829)
                     /locus_tag="ACG8N9_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4897796.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7973..8485)
                     /locus_tag="ACG8N9_002237"
     CDS             complement(7973..8485)
                     /locus_tag="ACG8N9_002237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897797.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8552..8695)
                     /locus_tag="ACG8N9_002238"
     CDS             complement(8552..8695)
                     /locus_tag="ACG8N9_002238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4897798.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(9178..>9887)
                     /locus_tag="ACG8N9_002239"
     CDS             complement(9178..>9887)
                     /locus_tag="ACG8N9_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFS4897799.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQC
                     TISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWT
                     KYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLE
                     QLLSTVWLSVRTAFFVG"
BASE COUNT         3043 a         2092 c         1594 g         3158 t
ORIGIN      
        1 aacttttctt tgactttcaa tatctgagtg cgtgtcgtat aggaaccgtt gcggataata
       61 cgaaccgatc gacagtgttc acaataggaa ggagagtcat ccaattttcc tttaataaac
      121 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
      181 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
      241 gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
      301 gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaagc
      361 aaaatcaatt ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc
      421 caagaaaaag acgttggtcg tcgatgctgt tctctcgcct actcctcgtg cttgtagaag
      481 ttgtggttct actgtggtag atggaaacgg gaaagcaatt atagtgaaaa atgggaagaa
      541 ggaaacgatt gtccgttttg aacaatacaa tcatatgcct ttggttacgc gcctaaaaaa
      601 gcaacgctat acctgtaaaa actgtcgtac ccattggacg gctcaaagtt attttgtccg
      661 actcagccat tcaatcgcag atcatgttcg atataaaatt gcttctttac tgactgaaaa
      721 agtatctttg tcttttattg cgaaaagttg tcaggtatct ttgactaccg ttactcgtac
      781 attgaaagag tttaaaagct atttaccaaa gccttctaag aagattctcc ccagagtatt
      841 gatggttgat gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc
      901 agatggtgaa acaggaaact taattgctgt cttgcctacg cgtaaattac ctcgattaac
      961 aagctatttc ttaggctgca ccaatccaga agaagtagaa ttcttagtca cagacatgaa
     1021 cgccgcttac ttccagctta ccaaacgtgt tctgccaaat gcgaaagtag tgattgatcg
     1081 gtttcatatt gtcaaacaca tgaatcaggc actcaatgag ttgcgtatcc gtgaaatgaa
     1141 tgagcttcgt aaagcaggac agaaaagtca ggcagaaaaa ctgaaaaaga actggcgctt
     1201 tctgctaaaa aatcgtgcaa gcatcaacca ttatgagtac aaaacatgga aaagtttccg
     1261 agcaccaaaa tacccatttc tcactgaagc aatgatgatt gatcgattgc ttgagttttc
     1321 tgcgcctcta aaggaggcgt atcccttttt tcatgaatta gtcgaagcct ttcgagacaa
     1381 agaccctgac ttatttttct ccttattggc agaacttccc gaaacgttag atgacggctt
     1441 tcgggagaag cttcaaaacc ttctgaccta tgaagaaggt atcaccagcg caatgatcta
     1501 tccttattcc aatggaaaaa ttgaagcgaa gaatacccac ataaagacaa tgaaacgagt
     1561 atcctacgga tttaaatcat tcgagaacat gagaattaga atctttttga tcaatcaatt
     1621 aatcaatgta agataacaaa aaatctgaac cagaatgtgg ttcattctga cccggatttg
     1681 attttgcaca actcatcagt ccttattgac aaagagccaa aaatctgagc cagaatgagg
     1741 ttcattctga cccagatttg attttgtaca acccatcagt ccttattgac aaagagccct
     1801 tctttttagt attattataa ttcttccatg cgttcttcca gtcgctgtaa atcgcttgga
     1861 cttaattccc caattttttt ccatttgacc tttttgttgt ttttgggtaa ttcgtttaga
     1921 gttcctacgt caatccaaga ttcttttttt agtcctgcct gtttccaatc ccttatttca
     1981 acgtatttca gctttatttt atcggattta gtatgatatt ttgaagtaat tgacagaaac
     2041 gaaattgtgt ccagtccatt ttttagaata tagacaggcc ggcgtttacc gccctctgca
     2101 gctgtgaaag caacgtagac ggttgctata tcattagtat tcatcttcat tgaaccattc
     2161 ttcaattttt tcgtcagtgt ctagtttttg gacaggcaat ttttccgcta atctttgaat
     2221 attatggatt cgtttacttt tttccgacaa ttcaatagaa aatgggattt ttccttgctc
     2281 aacaatttgc ttataaaaaa cggtgacggc tgttttgggg ctcatgccta gttcatcaat
     2341 aatgctttct gcttcatttt tcaatttttc gtccatagaa atttgtaact tagccatttt
     2401 ctccacctct ttctgacaaa cacattatat cacggaaaga gttcaaatgg aattaataca
     2461 actacttttg aactccttga aatgcagtgt atttatctaa atataaggaa aggatatagt
     2521 gctaaatggc cttgatagat agaaaagttt caaaatccgc cacagcgata attgaagcga
     2581 acactcaacg acacacagaa caggaaaaga ctcctaaaag aaccaaatta actaggacgt
     2641 gtctgaaaac tccacaaaga tgtggtatgc tctggtaaaa aacgaatggc acaaccacga
     2701 agaccgatat ggttcttgga aaactttctc cacccttttt gtaattggag ggatagtggt
     2761 ttattgatag cgattttcca agttcttcaa gtggaaccag acattgagca ccacggctaa
     2821 aaagtgaatg aagtctttat ttttaatcaa taatttttaa tgattaccag taattggttt
     2881 tttcatgcgc cctatttcta tttgatgtgg agttaaataa tgctgataag taaaaatttt
     2941 tccgtcaatt aaactgccac tagtgaatcc tgttctattg gtaaaaagag aatagtctgg
     3001 gtattcatct acaccaaatg ataaagattc agccataaac tgttttttac cagcaaattc
     3061 agcactgcta ccttgatgga tatgtcctac aaaaatacct aaaatatcac tttcggcaat
     3121 caattcagca aaaccttccg gaacttcagt tacaatagat ggatcttccc acgacaatgg
     3181 atgatgaacc aataaaatcg ttcccttttc agcaggtgta cccaaccact cttgtaattg
     3241 aatcatctgt cctatcgata tttttccttc gtgctgggta tcttgagcag aatccaagga
     3301 aataattcgt aacccatttt gattcgtaca atggatatac tctcccgttt cattgctttc
     3361 aacactcatt ccttcaaaga aaacactgcg acgatcatga ttcccacgac aaaaatagta
     3421 aggtgtggtt gggaaatatt gatccaacat tttccgtaag acttgataat cagaaatccc
     3481 cccttcatgt actaaatcac ctgttaatag aataaaatcg tacactgtta acgagagttc
     3541 ttgtaacaat ttttccaatg ccaactgtgg atttttaatt gttggaaata gttcagcagt
     3601 aaacaagtta gtttgatagt tttctctaaa atggatatca gagagatgca aaaagcgcat
     3661 ggctattccc tctttcttca ataaaataat caggatatcc taaattttct tgcgtctgat
     3721 aatcaaaaaa atgcattctt tcaggttcta tgcggagatt gactgcatca ccaatctgcc
     3781 aagtaggttt ttcattaagg acaatcattt ctataccatc gactttaacg gtgtacgcat
     3841 aactttgacc taatagctca cgatacttca cttgaccgtg taaatcgctt gtttcttttg
     3901 aaaaggttac gtgttctggt cttatgccta aatacaatga gccactaacc tgaagaagtt
     3961 gtttttgcca agctggattc aatgttaaaa attgactggc tacctttaat gttccttgat
     4021 cataagtaac tggcataata ttcatgcctg gcgaaccaat aaaatttgca gtaaaagtat
     4081 ttttaggttt cgtataaaca tttttcggcg tatcaatcat ctgaattcta ccttcattca
     4141 ataaaacaat ccggtcagct aacgtcatcg cttcaatttg atcatgagtg acgtagacaa
     4201 aagtttgttt ataacgttga tgaattttta ccaattcttt tcgtgcatcc aatcgtaaac
     4261 gcacatccaa atttgataac ggttcatcta ataaaaaata attactccgt ttaaccaccg
     4321 cacgagccaa tgctacacgc tgccgttgcc ctcctgacaa atctttagga agtcgatctt
     4381 ggtatggcgt taaacctaac atcttcaaag cttcttccaa acggtgctga atgtcagctg
     4441 tcttaatttt attcgccctt aatgcaaaaa taatattctc ctttacagtc atgtgaggat
     4501 ataaagcata attttgaaaa accattgcga tctctcgttc tccacaaggt agcttatttg
     4561 ctagttgtgt acctaaaaaa agctctccac tcgtaatttt ttccaaacca gcaatcattc
     4621 tcagcaaagt agattttcct gaaccagatg gtcccagtaa cacaattcgt tctccagcgg
     4681 ccacagctaa atcaatttct tttaaaatgg tatgttctcc atattttttt ccgatatttc
     4741 ttaattcaag cgtttctccc atgatttgtc ctccaattta tttaatacct gcagaaacaa
     4801 aagtcccgat aatatatttt tgaaaaattg catacaagac taacggtaca aacattgtaa
     4861 ttaaactaat cgccattgct acggtaaaat ttgtcccacc ttcagaacta atataggtct
     4921 gcatcgccaa cggcaaagta taattatcta ctgtccgtaa aattaatacc ggccaaacat
     4981 actcattcca agaggtaata aaaaaccaaa taccggtaga agtaatcgca tgtctagtaa
     5041 gaggaaaaac aattttactc atgacggttg tttgtttgat gttatccaat gccgccacat
     5101 ctagaaggct cactggaatt tcccgcattg tttgcgtcaa taaaaaaatg cccatcgcat
     5161 cagcaaattg tggtattatc acaccccaag tagtatcaat taaattaagt tttgcaatca
     5221 acaaataatt aggaatcatc gcaacagtaa aaggaatgaa catcgtacca atgatgataa
     5281 agtaccagat tttcttccct ttgattcggc aataagtaaa actatatgcc gcaaaaaaag
     5341 ctacgcctaa cttaacaacc gttataattg tagcaataaa aaaagtattt ctcgtaattt
     5401 tgataattgg aagattttcg aacaaatagc aaaaattttc caaggtaaac ggctgtggta
     5461 tcaaactagt aatcgtctga taggcatctt tcaatggctt aaaagaattg gccataataa
     5521 acaataatgg atagataata atgattatga tgaacataaa taagagatgt ggcaaccagc
     5581 gtttaatttt catagtaaac ccccttttcc acaaatttaa atattacccc taagaaaaca
     5641 gcaaatatta ataaagttaa aactgaaatt gctgaagatt ctccgacttt atacatttca
     5701 aaagcctttt gataagcata atagatcacg tttgcactac cattattcgg tccaccacga
     5761 gtgatgatct taatcggagt aaacacatac tgcagacctt gaacaatggt catcaccaat
     5821 aaatatagtc ctgtcgaact actcataggc aacacaattg taaaaaaaat gcgccagatg
     5881 ggtacacgat ccaacttagc tgcttcgatc atctcttgat caattcccac aattgctgca
     5941 aaaatgacaa tgaaattata accaaaaact ttccaagcag taatacctgc aatcacagca
     6001 attacccaac cttgtaaatt catccacata ggcagtctta aaccaaaatt tcccaacaag
     6061 acagcaattg gtcccgatac cggatttaat atccatgtaa aaaccatccc cgccactacc
     6121 aaagaaatca cagctggaat aaaaaataat ggtttataca aattctgccc tttttctata
     6181 actattccta ataaaaagga tgtaatatat gggaacacac agctcagcaa gacaaataga
     6241 acggtatagg acaaagtatt cttcaacact ttatgaaaga ctggatcagt cagcaccttt
     6301 acatagttgg caaagccaac aaattcttta gtgggggcca ccatattcca gtcaaaaaaa
     6361 ctcaaataga tcgtataaat caatggataa aaaacaaaca cgcccaacaa aataattgct
     6421 ggcgccagat acaaataacc tacatagcgc ttcatttccg acagcctcct catccttatg
     6481 tcaaaagcag acgaccttgt ttttcctttc ttacaggcgc tgtcgtctgc ttgctttgct
     6541 tatttgagta actcattaat ttccttttga gtagctttca tcgtttcatt gacggaatta
     6601 cctgataaaa ttttatcccg catatccaac aatttttgtt cagcttctaa tccagcatct
     6661 ccaggaaatg aactccaact gaccatctta tccatctgat caattgctgg tatcatcatc
     6721 tgattttcag ctaagaaagc ttttaatcca ttttgggcgt ctgcaacatc tttacgtggt
     6781 ggtaaataac cagtaccttc tgtccattta gctaaagatt caacactata aagatatttt
     6841 aagaaatccc aagttgcttc ctttttctta tccgtttgtg atgtaacagc taacattgcg
     6901 ccaccagcag gtagtttaac ttttttccct tcccaacttg gtgactcaac tgctttagca
     6961 gtaaaagtac tatttttttg aatgttgtcc ctttgagcaa tagtggtata gaccatcgcg
     7021 acgttaccat caataaagct ttgcacacct tgttcccacg tagtgtgtaa tgccgtctgg
     7081 tcttttacca tcctttgata taattcataa gcagcgatac catctttaga agcaaaggta
     7141 gctttgccat cttttattac acttgcccca ttactttcta aaattgcttg ttgtgcccaa
     7201 ttatcagcag gttcttgaat atacacgcca tactttccag ttttttcttt tattatctta
     7261 gaaaaagttt gaacttcttc ccaagttttt ggaccatttt catccaagcc cgctgctttt
     7321 aatatatctt gatttaaata taaaaccgga ttactaatgg agtaaggaat acccacttgt
     7381 ttaccatctt tttcagacag tgccatgata ttgtctaaaa aatgatcttt aaaataatca
     7441 gcattatccg gatccacttc ttctactaaa gattgaggat tagtatattt gaaattttct
     7501 gcaaaataat ctttaaaagc ccatcccact tgtactacgt ccggtatttc tttggaggca
     7561 acagaagctt gtaaattttg catcaagcct ttatacatat caggattata ttttgctaca
     7621 accttcactt tgtcttgaga ttgattgtat ttatctacta attcattcac tgtttttcca
     7681 cctagggtat ccgcattaac atgccaatac tctatcgtta cacgaccctc cgattttttt
     7741 gttgtcttgg tattcccact accacaagct gccaaagaga tgacacttaa aacagacaca
     7801 acccctataa caagattttt ctttaacata acattaactt cctcctaatt tctttatggc
     7861 catttttcta atcaagaaat attatgaact accaatgtaa atttctagtt aattttctgt
     7921 aaataattcg aaatttgtgc atttcggggt acagggtcac tggacatatc catcaagcta
     7981 agaataaagc ccccatatgg tcatgtatcc catctccaaa tctcttaact cctctgtttt
     8041 gattataaaa tagtttattc tgtgctcctt tcttccataa ataggcccat gcacaacgtg
     8101 aggttatatt ataaacacca cagcgcgcaa ggtactcaat cgtttctagt tcattgactg
     8161 tccatacaga aaatgtcaat cctagctgct gccccacttc acgggccaac ttagttaacc
     8221 atccttcatg aatattgacg atttgaacgc tttgataggc taatttctgt aaggtatcga
     8281 tcaaatctgc ttcggtaata tccccaagtt ttttcccttc gaactgattt tcaatattat
     8341 aaaatatatg ctttccccaa atcgcagccc aagataattg tacatagcca ctaagaacaa
     8401 tttgtggcca gatttgatag gtttgtgcca gcgaaaaaac ggccatttcc aatccctctt
     8461 cttttaaatc acagttaatg gtcatggttg ccttctcatt ggccactgct tgaaacacct
     8521 gctcaagggt taccgaatct gggacaactt cttacctgtc atgggctagt attagcgtac
     8581 catctgtttg ctgacgcaca tctacttcaa aacaaagctg gcgatccaaa caaaagcgaa
     8641 gaaatgccca gctattctcc tccgtattgt cactaccaga gtgtgcagtg atcatggtca
     8701 ttttgacccc tcctttaatt ccgtgatttt ctcccacacg tccactatac cgatgaagcg
     8761 ttaatttctt gttaatttct tgtaaattcc ctgcgttttt tcagtaaact agttgtaaat
     8821 cttgcatgct ccttcctacg aactcatgaa tcgtcgattt tgcccttcta gctagtttca
     8881 ccaattaagt agttctacat tccccgcttt taacctacta cacctttttt tgttttcagc
     8941 tattccttct tcttgctgaa agtccttgag gttctttctt acaggatacc atcaaggtgt
     9001 tcaatggctt attcccattg aatgaccgag acttactatc agatttctgc tcgttgaacc
     9061 aagtctttga tacaggattt cttccgtcat cttctcacaa ataccacctt gcgtttctct
     9121 agcgattggt cgatgtgcac cctcccagtg gactgtcact accccttttt gctatgccta
     9181 gcccacaaaa aaagctgtcc tcacagacag ccagactgta gacaaaagct gttccaaact
     9241 cgtaactcga gcttggaaca gctttttatc tacttcgttt cgaatttttg aacaaaaaaa
     9301 taaaggctcc ttgcctttcc atagccaggt ggctaatttc tttaaattca ttgcagcaaa
     9361 cactagcatc gtatgcgtcg cgactttttc caagcctcga tacttggtcc aacgcatccc
     9421 atgcttttct tttgcatccg caaatattcg ttcaatcgtt tgtttccgtt ttttgtaaat
     9481 tgatttattc agatctgtta ggcgaagatg ttccacttca tccatcagat ctccccaaac
     9541 atgtctcgtg atgactcgtt gatgattttt acttatggta cattggttta gcagaggaca
     9601 gttcacacat tcttttggat tggatttgta ttctcggtac ccttctcgag taaccgttga
     9661 aaaagcgagc atttgatctt gcggacaaat ataacaatca aaatactcgt catagagaaa
     9721 ctcatttttt gataatagcc cctttttccc tttgggcctt gaataaggca aacaaggtcg
     9781 aaggttcaaa tgagtcaaaa agtgaacaaa cttaggtgtc ttatacccag catcagcgac
     9841 aacacaattg atttcaggaa aaagtctctt cgattgtttg acgagct
//
LOCUS       DAZUKD010000105         9853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000105 DAZUKD010000000
VERSION     DAZUKD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.107"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..182
                     /locus_tag="ACG8N9_002240"
     CDS             <1..182
                     /locus_tag="ACG8N9_002240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897800.1"
                     /translation="SPFKRRKCLFIKKRKRYNEKEESFKNLKKEEGIGKNELFEQKFY
                     KMRVIIFVLILFLIK"
     gene            232..1755
                     /locus_tag="ACG8N9_002241"
     CDS             232..1755
                     /locus_tag="ACG8N9_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4897801.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1992..3332
                     /locus_tag="ACG8N9_002242"
     CDS             1992..3332
                     /locus_tag="ACG8N9_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4897802.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3486..4223
                     /locus_tag="ACG8N9_002243"
     CDS             3486..4223
                     /locus_tag="ACG8N9_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4897803.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4388..4609)
                     /locus_tag="ACG8N9_002244"
     CDS             complement(4388..4609)
                     /locus_tag="ACG8N9_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4897804.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4640..5671
                     /locus_tag="ACG8N9_002245"
     CDS             4640..5671
                     /locus_tag="ACG8N9_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897805.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5658..7061
                     /locus_tag="ACG8N9_002246"
     CDS             5658..7061
                     /locus_tag="ACG8N9_002246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4897806.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            7127..7762
                     /locus_tag="ACG8N9_002247"
     CDS             7127..7762
                     /locus_tag="ACG8N9_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4897807.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7810..8490
                     /gene="cmk"
                     /locus_tag="ACG8N9_002248"
     CDS             7810..8490
                     /gene="cmk"
                     /locus_tag="ACG8N9_002248"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4897808.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            8625..>9853
                     /gene="rpsA"
                     /locus_tag="ACG8N9_002249"
     CDS             8625..>9853
                     /gene="rpsA"
                     /locus_tag="ACG8N9_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4897809.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         3290 a         1662 c         2124 g         2777 t
ORIGIN      
        1 cttctccttt taaaagacga aaatgtttgt ttataaaaaa aaggaaacgc tacaatgaaa
       61 aagaagaaag ctttaaaaac ctgaaaaaag aggagggaat agggaaaaac gaactttttg
      121 aacaaaagtt ttacaaaatg cgtgttatta tttttgttct gatactcttt ttgataaaat
      181 aacattagag ccaaaattgt tgtctctaac ggaaaagaaa ggaagaagaa gatggaacaa
      241 acaaaaaaga aaaaatttca aatgccttca gcgtatacgg tcttatttat tattattgca
      301 gtaatcgctg tacttacttg gtttatacca gctgggcact atagtgtaga cgatgcaggc
      361 aacatcgtcg ctggatcata tgaacgagta caacaaaatc ctcaagggct gtgggatatt
      421 ttcatggcgc caatcaatgg gatgttaggt gtaccagcgg gggagctaac taaagaaaca
      481 cctgctgcaa tcccaatctc tttcttcatt ttggttgttg gtggattttt aggtattatc
      541 aacaaaacag gggcactaga tgccgggatt gcttcagttg tgaagaaatt caaaggaaaa
      601 gaaaaaatgc ttattccagt attgatgctg ttatttgcat taggcggctc tacttacgga
      661 atggcagaag aaacgattcc attctatgct ttactgatcc cagttatgat ggctgtaggc
      721 tttgatacag tagtagcagt agcaatcgta ttagtagggt ctcaagtagg ctgtctagct
      781 tcaacggtaa atccttttgc aacaggcgta gcttcacaag ctgttgggat ttcgatgggt
      841 gaaggtattg gtcttcgatt cttgatgttc gttattttgt tagctatttc tattgtatat
      901 gtgtatcgtt atgcatcaaa gatcgaaaaa gatccaacaa aatcattagt atacaaccaa
      961 cgtaaagaag atatgaagca tttcgatatt gaagcaatcg gtcgtcaaga agtaatcacg
     1021 aaaaaacaaa aacatgttaa tgtattgttc tttattactt ttggtatcat gatcatcagt
     1081 ttgattcctt ggacaacctt gaatgcgaat ttcacgattt ttgaatcatt gacaaactgg
     1141 ttaacaaacc ttccggtact aggtactgtt ctaggtaaaa gcatgcttcc attaggtgac
     1201 tggtacttcc cagaaattac tatgttattc ttagtaatgt ccatcgtagt agcaatcact
     1261 tatggcatga aagagtctga atttgtctct gaatttttag ctggtgcttc tgatttgatc
     1321 ggtgtagcaa tcgtagtagc tgttgctcgt ggtatccaag tggttatgaa caatggatta
     1381 atcacagaca ccatcttaca ttggggcgaa caaggattaa gttcattatc ttcaagtgta
     1441 ttcatcatta ttacatttat tttctatatc cctatgtcat tcttgattcc ttcaacttcc
     1501 ggcttagctg ctgcaacaat gggaatcatg ggacctatgg gtgaatttgc cggcgtcggt
     1561 aaggatttag taatcactgc ttatcaatct gcatctggtt tagtaaactt aattacacca
     1621 acatctgcta tcgtaatggg tgctgtagca attgctcgtt tcgatgtttc agtgtggtgg
     1681 aaatttaccg gtaaattaat tgcgatatta tttgtagcca tctgtgtgat cctaggtgta
     1741 gcagcaatgt tctaaaccta gacaaataca ggtacaaggt ctatcataag aatgaagaaa
     1801 ccgataaacg gctatcgata agcacgtgga caagtcaata gctccattca gtgagtgttt
     1861 tttctttcat catttgttaa gaacattctg cttgctgatg gagcaggctt aaaacgtcat
     1921 tatttgagaa gaagaggttg tgtcggagga atttgacatg acccttttta acatattagg
     1981 aggataatat catgaaaaaa tttgtgacag aacaacatca tgaacaagca ttagaatcat
     2041 tgagcgaatt gatccgaatt ccttccgtac tagatgaagc agattcagga caaggacatc
     2101 ccttcggtaa aaaagtgatt gaagctttgg ataaagtatt ggaaatcagt gaaaagctag
     2161 gatttagaac atttaaagat ccagaaggct attacggtta ttcagagatt ggctctggag
     2221 atgaattatt cggtatcttg tgtcatatgg acgttgttcc agcaggggac gaaaataatt
     2281 gggaaacaaa accattcgat ccaactataa aagacggctg gttagttggt cgcggatccc
     2341 aagatgacaa aggtccttct attgcggcaa tgtatgccgt caaagctttg attgatgctg
     2401 gcgtggaatt caagacacgt attcgtttta tctttggtac agatgaagaa aatttatggc
     2461 gttgtttaga aaaatataac gaaaaagaag aaggaattac gcaaggattt gcgccagatg
     2521 ctgaatttcc tttaatttat gcagaaaaag gcttattgca agcttacttg acaggaccag
     2581 gaacgaacga gttttctgta aaagctggag gagcgttgaa tgttgttcct gatgcagctc
     2641 cttactctgg agaaaagctt tctgaagtaa aagaagcgtt gaaaaaacac gaattcgatt
     2701 tcgaagaaca aggtgaaggc atcgtcgttc ttggtaaaag tatccacgcg aaagatgcag
     2761 cacaaggagt gaatgctatt tctcgtctgg caattgctct ttcagaagta ttcgattttg
     2821 gcccaatcaa tttcttagga aaattagttc aagaaaatgc aactggtgaa gctgtcgtgg
     2881 gcaaaacaga agatgaacaa tccggcgagc taacgatgaa ctttgcaagt ttggaaatca
     2941 caccagaaca aacaaaaatc ggtgtagata tgagaatccc tgttacattc aagaaagatg
     3001 atttagtagc taaattaact aaaacagcag aaaaatatgg gttgacttat gaagaatttg
     3061 acttccttga ttcattatac gtgccactag acagtgagtt agtgaaaaat ctattgggga
     3121 catatcgcga tattactgga gacatgactg aaccgtttgt atcaggcggc gctacttttg
     3181 ctcgtacgat gaaccagtgt gtggcattcg gtgcgatgtt ccctgatact cctgacttta
     3241 tgcaccaggc gaatgaacga tgggaattaa gtagtatgta taaagcaatg gaaatttatg
     3301 cagaagctgt gtatcgttta tgtgctaaat aaaaataaac atacaaaaag ggcggatatt
     3361 caaatggata ttccgtcttt ttttatttga tgctggaaag tttgcatttc tctcttattt
     3421 tttttaaggt ataatcatac ttgattaaga aaaaggggtc actcagatag aaaaggtggg
     3481 agaaagtgaa aatatttaga cgaatactaa ttggcttagt catcatttta ggattactcg
     3541 gtggagccgg ttattactat atacagcaaa atacgtacga accatcgtca gaagcgcaag
     3601 tgttggtaaa agaaggaaaa aacaaagaag attacttatt ttttgaagga gagaaaaaga
     3661 atcatggaaa ttctccatta cttatttttt atcctggtgc tctcgtagag ccagaaagtt
     3721 atagtatttg ggcgcaagat ttagcaaaaa acggcttttc agtcgctatt gtcaaaatgc
     3781 cacttgatct agcagtgatg ggcggaaaca aagcggataa ggtcaaagaa gattttccaa
     3841 attccgatta tgtgatcggt ggccattcat taggtggcgt aatggcaagt cgttatgtca
     3901 ataagaatca ttcagacaaa catttaaaag gggtattctt catggcaagt tatcccgaca
     3961 aaaaaggaac attgacagaa tcagctgttc ctgttttatc gatcacggcg acaaaagata
     4021 aagtattgaa tcaggaagct tatcaagaag ctaagcagta tttaccggaa agaacagagt
     4081 atgtatcaat caatggcgga aatcatggag gttttggcag ctatggtgag caaaaaggag
     4141 atggagaggc agaaatttct aacgcagacc agcaaactgt gatcgtcaat acattggaaa
     4201 actggctgaa aaagttgaaa taatataaat gggtcctata gggtccgtga aaaaactagt
     4261 caattaacta tagatagcaa atgaatcttt caaaataagt cgggatttca cggaacaaga
     4321 gaaacagatc ccggcttatt ttcaagttta gagaacttag tgtacgtttt taaagtttcg
     4381 tttatattta tattttattt tctaataaaa tcgctcgaac tggacatttt tgatacgctt
     4441 tttttacata tgtttcttct tccggcatca cgaactgttg atgagcctct ggttcttggg
     4501 caaataaaac gattccttct tcatcataat cgaaaatatc tggcgcttcg atttgacaaa
     4561 gaccgcatgc aatacagcgt tcaggtacaa gtttacatag tagtgacatt tctaaccatc
     4621 ctaacttaag gagaataata tgattgtttt tattttatcg ctattttcat caggacacaa
     4681 gctgcggatt agttctcttt accaactcct tgtgggaaaa cgaacgactt cagtacttat
     4741 ttatggattt acccacgaac ttttgtttat ccataacagt tttccagaat tgaagcagga
     4801 taagttttat caaattttgc aaaaaattgc gcagcaaggt tggatcgaaa taaatgaaaa
     4861 tgaagcaaag ctaacgcctg caggggctga tatgttgtcc gaacatcaaa tagaacatac
     4921 tggtttgcgt tttgaccgct atggcagaac aggggaaact agttggcggc tcattaaatt
     4981 tgctgttcaa gtaatttcta atctggcaac gggtattcaa gactatcttc cagcagagac
     5041 aagtcctttt tatactttcc aactaaaaaa atggcttagt gaaagccaat tgccacgtga
     5101 aattttgatt gatactgcgt atgagagttt ggtgcagctg ttttcggaga taccagaaga
     5161 agcagcagat tttttagcta accaattatc tggaaatgag cgaacagggc tgcttcctta
     5221 tcagctggca aaaaacaatg atgagtcagc ggtttatctg caacaaagca gatgtatcca
     5281 ccttttgtta gcacaaatcg aaaaacggcc agattcgctt tggcacgctt tgatcgattc
     5341 attattacag caaaatttca atcaaagcat gatgatcacc aagcagatgt ttatgaatgg
     5401 tcagacaatt gaccagatta tggcaatccg tcatttaaaa agaggaacag taacagatca
     5461 tcttattgag tgggccttgt tttttgatga ttttccatac gagcggattc tctctgcaga
     5521 aacaatcgaa cgtttggaac caaataagga ttccgtgcga gagtggcgct ttagcgaatg
     5581 gaatgtagat ggacaattag attatggaga atttcgtttg tatcaaatct atctattgag
     5641 aaaggaagcg atacagaatg ttaataaatg aattaaaaaa atattttgga tttgattcat
     5701 ttcgtcccgg ccaagaagaa atcattcagg cgttgcttga tggagaagat actctcgcta
     5761 ttttgcctac aggaacggga aaaagccttt gctatcaatt gacggggttt ttaatggaag
     5821 gtctggtcat catcgtgtca ccgctgcttt ctctaatgga agatcaagtg acgcaattac
     5881 aaaaacgagg ggagaaacga gtagctgctt tcaatagttt actttcgcaa tctgaaagaa
     5941 gatacgtcct ccgtcatcta tcgcaatata aatttttgtt tttgagtccc gaaatgttga
     6001 ctaatccttc tcttttagag catctgaaaa aacaaaatat tgcgttatat gtagtagatg
     6061 aggctcattg cgtgtctcaa tggggagttg atttccgtcc agaatatcaa cagttaggaa
     6121 aaatccgaaa gcttttagga aacccggtca cactggcact aacggcgaca gcaacagatt
     6181 tagtagcaag agatatacgt catgtactat ttgatcgacc accaaaagag atcagacaat
     6241 cagtcaatcg gcaaaatatc tcgctttttg ttcggaaaac gcagcaaaaa gaacaagaac
     6301 ttgaacactt catggaaaga gcacaaggag cggcaatcat ttattgtgca acaaaaaaag
     6361 aagtcgaacg attgtatcat ttattcaggg aacgttttac agttggatac tatcacggtg
     6421 gtcttgatgc agctcaaaga agacaacttc aacagcagtt tgtaaaaaat cagctgcagt
     6481 ttctcattgc tacgaatgca tttggtatgg ggatcgataa atctgatatc cgttatgtga
     6541 tccattatga tttgccagac agtctagaaa actatgtaca agaaatcggt cgagcaggca
     6601 gagatcagga agaaagtgca gcaattcttt tttatcaaag tggggatgaa cgtatccatt
     6661 atttctttaa tcaactttct agagaacagc gacaaagttt tgagctttat ttggaatatg
     6721 ccacagagca agctcccttt gatgagctgc aaaaaaaatg gatggagctg atccagcagt
     6781 ctgaaaaacc agaaaattgg gtggaacggc tcaaacatca ggaaaaagaa aaagaattcc
     6841 gcctgcagca gatgcttcgc tatattaatg aagaaaactg ccgcagaaaa ttcatcttag
     6901 cttattttgg cgaaaaactc tccgaaaagc cgcagaattg ttgtgatatt gatggagcaa
     6961 aagcagaact tctgccaaag aggaagcttt tttctaagac tgaacaactt cattgggaaa
     7021 cgattttact aaatttattt aaaaaaaaca tggaacattg attttttagg acaagacact
     7081 ttttcctatg gtataataca cacgatagag tttaggaggt aaaacagtga gcagaaaaga
     7141 tagacatcaa tctacagaaa ataacgaaac acaagaacca tgggaacaac caatctacga
     7201 tacagatgat gaagtttcat caagaagttc tcagagacaa caaaagaaag gaaatacatt
     7261 atttctttcg ctatttttga ttttactggt cttatgtatc gctattccag caggtgctta
     7321 tttctggatc agaaatggat caagcaacaa tgcaacagca gcaacttctt ctgaaacttc
     7381 gacatcagta gtagaaagtt ctacaagtac aacaaaagag gagtcaactg agacttcaac
     7441 agtagaatct agtacaatcg cggaagaatc tactgtgtct caagatacag gtgtaacgtc
     7501 agaaagcagt attgcagcag agacaacacc gacttcctca agtgaggcaa cttatcaagc
     7561 aggggattca agcagcaatg cagcagaaac aacaccttct tcatcagctg cagccggttc
     7621 aacaacgact gttcaagcag gcgaaggtcc aaaacaagtg gctgagcgtg cagggatcac
     7681 cactgatcaa ttgtttgaat tgaatggctt agatccaaac aactttatgc tttaccctgg
     7741 tcaagaatta agaattaaat aataatagcc gcgctttgcg gctttttttt gagaaaaagg
     7801 agttatatca tgacacaaat cagtattgca atcgacgggc ctgcttcatc tgggaaaagt
     7861 accgttgcca agatattagc aaaagaattg aattatattt atacagatac cggtgccatg
     7921 tatcgcgcag taacttattt agctattacg aacaatgtat ccttttcaga tgaagaaggg
     7981 ctagtttcgc tgatcaaaaa gtatcctatc tctttttcgc aacaagaaga tggacagcat
     8041 gtattcatag catcaaaaga tgtgacttta gaaatccgtc aaccagatgt cacaaaagct
     8101 gtttctgaag tctctgcaca tggaaaagtc agagaagaat tagttgctat ccaacgaaaa
     8161 ataggggaac gcggtggcgt tgtcatggac ggaagagaca tcgggacagc tgtattaccg
     8221 aatgcagaag tgaaaatctt tttagtagca agcgtaagtg agcgtgcaga aagacgtttc
     8281 aaagagaacc aagaaaaagg aattccgact gatcttgaaa cactgacgaa agagatacag
     8341 gaaagagacc attatgattc aacaagggac gtctctccgc tgaagcaagc agaggatgct
     8401 gttcgtatcg atacgactgg caaatcgatt ccagaagtcg ttgcagcaat aaaagctgtt
     8461 gttttgaaaa aaggttatct tcttttttaa attaagaaaa aaaatgaaaa acaattgttt
     8521 tttcaaggaa aacgaacgaa atcgctttat tttttttgaa taatcaatta aactaaaaac
     8581 agtaccgtac ttacggcaga atgttggtta ggaggacaag tgttatgaca gaatttgatc
     8641 aaaatcaaga gaacaaccag tcaatggaag acgcaatgaa aagcgtacaa gaagtaaaag
     8701 ttggcgatgt tgttaagggc gaagttttag tcattgaaga caaacaagtg attgtcggaa
     8761 tcgaaggggc tggagttgaa ggagtcgttc cggcaaaaga attatcaact ttgccggtag
     8821 aagatatcag cgaagtagta aaagtcggtg atgtattaga cttagtcgtt atcagtacga
     8881 tcggcaaaga taaagaaaac ggcagctacc tactttcaaa acgccgttta gatgctaaaa
     8941 aagtttggga agatatcgaa agagatttcc aagagggcaa ggtgatcgaa gcacctgtta
     9001 caaatgttgt caaaggcggt ttagtcgttg acgtcggtgt acgtggtttt gtccctgcat
     9061 caatggtgga agatcatttt gttgcagact tttcagacta taaaggcaaa acattgagct
     9121 ttaaaatcat cgaaatcgaa ccatcagaaa atcgtttgat cttatcgcat aaagcggtag
     9181 tggaaaacga aagagaagaa aagaaaaaag aaattctttc aactatccat gagggagatg
     9241 ttctcagtgg acgtgttgca cgtcttactg attttggcgc atttgtcgac ctaggcggaa
     9301 ttgacggtct agtccatgtc tcagagattt cacatagtca tgtagcaaaa ccaagtgatg
     9361 tattggctgt caatgatgat gtaaatgtca aagtgttgtc tatcaatcct gaacaagaac
     9421 gtgtttcctt gtccatcaaa gacacacagc ctggaccttg gtcagaaatc gatacaaaag
     9481 ctccagttgg cacagtatta gacggaacag ttaaacgttt gacaagcttc ggtgcctttg
     9541 ttgaagtgtt ccctggagta gaagggctag tccatatctc tcaaatttca cataaacata
     9601 ttgcgacacc acatgaagtg cttcatgaag gtgacgaagt gaaagtgaaa gtattagaga
     9661 tccatccaga agaacatcgt attgcattga gtatcaaagc tttagaagaa aaaccagctt
     9721 cagaagaaga accaaaaaat gttgagtctt atgaattacc agaagaaaat actggattta
     9781 ctatgggaga cattttaggt gatgcgttga aagaagaagt agaagaaaaa gcaaccgatg
     9841 attctgaaaa ata
//
LOCUS       DAZUKD010000106         9782 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000106 DAZUKD010000000
VERSION     DAZUKD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.108"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..3038
                     /locus_tag="ACG8N9_002250"
     CDS             231..3038
                     /locus_tag="ACG8N9_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4897810.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3229..3720
                     /locus_tag="ACG8N9_002251"
     CDS             3229..3720
                     /locus_tag="ACG8N9_002251"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4897811.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3823..4812
                     /locus_tag="ACG8N9_002252"
     CDS             3823..4812
                     /locus_tag="ACG8N9_002252"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4897812.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4845..5648
                     /locus_tag="ACG8N9_002253"
     CDS             4845..5648
                     /locus_tag="ACG8N9_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4897813.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5669..6583
                     /locus_tag="ACG8N9_002254"
     CDS             5669..6583
                     /locus_tag="ACG8N9_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4897814.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6666..7040
                     /locus_tag="ACG8N9_002255"
     CDS             6666..7040
                     /locus_tag="ACG8N9_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4897815.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7179..7424
                     /locus_tag="ACG8N9_002256"
     CDS             7179..7424
                     /locus_tag="ACG8N9_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4897816.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7596..8660
                     /locus_tag="ACG8N9_002257"
     CDS             7596..8660
                     /locus_tag="ACG8N9_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4897817.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            8906..9331
                     /locus_tag="ACG8N9_002258"
     CDS             8906..9331
                     /locus_tag="ACG8N9_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4897818.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9324..9773
                     /gene="nusB"
                     /locus_tag="ACG8N9_002259"
     CDS             9324..9773
                     /gene="nusB"
                     /locus_tag="ACG8N9_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4897819.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
BASE COUNT         3189 a         1613 c         2172 g         2808 t
ORIGIN      
        1 tatatggtaa acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc
       61 gttcttttaa attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta
      121 aatagttgac gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag
      181 tctatagaaa aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca
      241 aagaaaaaac cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa
      301 ttgctgaagt gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc
      361 gcgaaggaaa actgataaag acagacggac gaccagtacg ttacatatat caaacactcg
      421 ttacgcacgt aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg
      481 aagaaaaaaa tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta
      541 acggcagcat gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag
      601 gattaaattg tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga
      661 tgtatcattt tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca
      721 attgtgctga ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa
      781 aaggtgcttt tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg
      841 gtatgctgtt tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt
      901 actttatgga tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa
      961 atgtacggat cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg
     1021 ttcggcgtat cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga
     1081 aagtcgattt agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt
     1141 ctttgacaga agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac
     1201 aattgaagtc aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac
     1261 ccgaaatctc gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat
     1321 tagcaagcaa ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg
     1381 tagcaccaaa tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc
     1441 tatatgatat catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag
     1501 caatcaatca tttcatttct acagatatca acatccatct gaagtctttc tataaagacc
     1561 atggtttttc ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag
     1621 aagtgaccaa tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa
     1681 actttattta tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag
     1741 aagaacggca tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg
     1801 ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa
     1861 gtgaagacta ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca
     1921 gaatcggtgt agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg
     1981 tccaacagtt gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc
     2041 cgaaaacagc attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg
     2101 tcatactctt agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca
     2161 ctggaattcc agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac
     2221 gaaaggtctc agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc
     2281 gaggttacgc agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga
     2341 aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat
     2401 tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga
     2461 ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta
     2521 ctgggattac tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc
     2581 aaaatatcga agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat
     2641 tcatctccct agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact
     2701 tcatctttat taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct
     2761 ctcagacgac gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca
     2821 cagcaggaat gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat
     2881 tgacaaatac aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg
     2941 aagatcagat aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc
     3001 ataatcagtt agataaaaaa gaatacactg tgccatgaat acacaaaaac gatacacttt
     3061 tatagtgtat cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta
     3121 aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt gttcacacag
     3181 aaatgatagg ggtttctgtg aagtagtgag gaagaaggag ttttgcagat ggatattcga
     3241 ttggcacgta tagatgatcg tttaatccac gggcaagtcg caacagtttg gtcgaagcaa
     3301 acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa attgagaaag
     3361 tttctactaa aagaagctgc accgccgggg atcacagcaa atgtaataac cgtgcagaaa
     3421 atgattcaaa tctatcatag tggattatta tcacgagcaa aagtgatgct tttattcacc
     3481 aatccccaag atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc
     3541 ggcggtatga gttttactga aggcaaatca atgattacca attttgtttc agtaaatcaa
     3601 caagatattg attcctttcg ttacttgaac agtcaagaag tggagttaga gattcgaaaa
     3661 gtacctgctg acaggaaaat ccttttgatg gaactactag cgaaaatcaa aaaggtgtag
     3721 caagaatggt tgttggaaaa ggtcaaataa ttctgttgta atggttagca attattaata
     3781 tttttgaatt tgttacaaca aaagtaaata ggaggtataa atatggtagg gattatccta
     3841 gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa
     3901 caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag gtccagatga cttgagagcc
     3961 aaattggaag aagcggttgc ttcttttgat aatcaagacg aagtgttatt cttagttgat
     4021 ttatggggcg gaacaccgtt caatcaatca aacacattat tcgaagagca caaagataaa
     4081 tgggcaatcg tcagcggttt gaatttacca atgttgattg aagcctacgc atcacgcttt
     4141 tcaatggaat ctgctcatga aattgcagca cacatcattg aaacagcaaa agacggtgta
     4201 aaagtaaaac cggaagaatt agaaccggca gaagcaccga aagcagcagt tgaagacgca
     4261 caacctaaag gagcacttcc agaaggaact gtggtcggag acggaaaaat caaatatgta
     4321 ttagcacgtg ttgactcacg tctgttacat ggacaagttg cgacagcttg gacaaaagca
     4381 gtgcaaccaa accgtattat cgttgtttca gatgcagtat caaaggatga tctgcgtaag
     4441 agactaatcg aacaagctgc tcctccaggt gtaaaagcaa atgttattcc tattagcaaa
     4501 atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca aagcattgct tttatttgaa
     4561 aatcctgaag acgtattgac agctgttgaa ggtggcgtag acatcaaaga attaaatgtt
     4621 gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta aagtattgtc tatgggacaa
     4681 gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa
     4741 gtacctaatg attcacgcga taacatggat gatattttga aaaaagcaaa agctgaatta
     4801 gcaaaagcgt aataaataat attaaaattt ataggaggct tatcatgtct attatttcaa
     4861 taattttagt cgtattagtt gcctttctag ctggtatgga aggtattctt gatgaatttc
     4921 aatttcatca accactagtg gcatgtacat tgatcggttt agttaccggt aacctcgaag
     4981 ctggtatcgt acttggcggt acgcttcaga tgatcgcttt aggttgggca aatatcggtg
     5041 ccgctgtagc accagatgct gcattagcat cagtagcatc agcaattatt ttagttttag
     5101 gtggacaagg tgttaaaggt gttccttctg cgatcgcgat tgccgttcct ttggctgttg
     5161 cagggttgtt cttaacaatg atcgtacgta caatcgctgt tccaatcgtt catttgatgg
     5221 atgcggctgc tgaagaagga aacatccgta aggttgaaat gtggcacatc attgctgtat
     5281 gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt gctatttatc ccagcacaaa
     5341 ctgtgcaatc attccttgaa tcaatgccag catggttaac agatggtatg gctatcggtg
     5401 ggggaatggt cgtagctgta ggttacgctt tggtaatcaa catgatggct acaaaagaag
     5461 tatggccatt cttcgttatt ggtttcgttg tagctgcaat ttctcaatta acattgattg
     5521 ctctaggtgc tttaggtgtt gcattagctc ttatttactt gaacctttct aaaatgggtg
     5581 gttcatcaaa taatggaggc ggaagcaact ctggtgaccc tttaggcgac attttgaatg
     5641 actattaatc ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa
     5701 agatcgttta gctgttgcgt ggcgctctac cttcattcaa ggttcatgga actacgaacg
     5761 tatgcaaaac ggtggctggg cattttctat gattccagca atcaaaaaat tatacaaaac
     5821 aaaagaagaa cgttctgcag cattaaaacg tcacttggaa ttctttaata cacacccata
     5881 cattgcttct ccaattttag gggttacatt ggctctggaa gaagaacgtg cgaatggcgc
     5941 gcctgttgat gatgtagcga ttcaaggggt taaagtaggt atgatgggtc ctttagctgg
     6001 tgttggtgac ccagtgttct ggtttacagt acgtccaatg ctaggtgcac taggtgcttc
     6061 attagctatg ggaggaaata tccttggacc aatcatcttc ttcttaggtt ggaacattat
     6121 tcgttgggca ttcatgtggt atacacaaga atttggctac aaagctggtt ctaaaattac
     6181 tgatgacctt tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat
     6241 gttcgtatta gctgcccttg tacaaagatg ggtatcaatc aaattcttac cagttgtatc
     6301 aactgtaaaa ttagataaag gtgcttacat cgaatgggat aaattgcctt caggtggtga
     6361 aggaatcaag agtgcatttg aacaagttaa taatggctta gccctttctc ctgaaaaggt
     6421 aacaacatta caagacaact tagaccaatt gattcctggt ttagctgcat taggattaac
     6481 attgttatgt atgtggttgc ttaagaaaaa ggtttctcca atcgttatca ttcttggttt
     6541 attcgtagtc ggtgttgttg gacacgtcat cggactttta taatccataa aaatatagct
     6601 cgggcgttgt cccgagcttt ttttatatag tatactgttc ataaacaaat atctggaggg
     6661 agaaaatggt tcaatcaatc aatacaaaag tagacttggt aattgatgct acttcgttta
     6721 cgggtctgac agattacggg caaatcatga taggtgataa tggttttgag ttttaccatg
     6781 cgcgagatag acgcaaatat atccaaattc catgggaaga agtggattat gtactagcat
     6841 cagtcatgct aaagggaaaa tggattcctc gatatgccat tcaaacgaaa aaaaatggta
     6901 catacacatt ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag
     6961 atgaagatag aatggtacaa tcattgagtt tctttgatgt agtgaaacgt ggtattcgta
     7021 gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa gaggaggttt tctttatttt
     7081 tttactttgc agaagaaaat atgaaatagc aaaaccaatt atttgtcccc tttaaatccc
     7141 atatgctaaa attgattagc aaagaaggag gttatattat gagttggtta tggacattga
     7201 ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga aattcgatgg
     7261 gctgtgtaac gaatatcatt gctggactag tcggttcaag tattggacaa gcgattttcg
     7321 gcgaatgggg accaaaagct gcaggtatgg cacttattcc atctatttta ggcgcagtga
     7381 ttttggtagc agttgtctca tttttctttg gaaaacgatc ataagttttt acaaaatagt
     7441 aaactaaaag gagttgcagc gagactgtga ctcctttttg gctttattta tttaaaataa
     7501 aaagaagttt tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt ttgctataat
     7561 ggaaaaaagc gtgcaaagaa agagggagtt tcattatgat gctacgtgta gaaaaattgc
     7621 gcaaaaaaat gcaagaagaa aatttggatt catttttggt taccagtccg tacaatcttc
     7681 gttacttaac gaactttaca ggtacgacag gattagctgt tattactttg gaaaaagcat
     7741 tttttattac tgattttcgt tataccgagc aggcggcagc gcaggctcaa gggtttgaaa
     7801 ttattaaaaa tgtcggacct atttttgatg aggtagcaaa cttagtacgc aaagaagaac
     7861 tgcactcatt agcttttgag gaaacaacag tctcattttt agaatattcg gttttagaag
     7921 aaatcatcga tgcggaatta gtacctgttt cacagatgat cgaagaactc agagaagtga
     7981 aagatgaaga agaaatcgct attatcgaaa aagcatgtca tattgcggat atggcatatg
     8041 atcacatcct gaaaatgatc caaccgggca tgactgagat tgaagtcgca aatcaactag
     8101 acttttatat gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg
     8161 gattgcgttc ggctatgccg catggtgtag caagcaaaaa aatcatcgaa caaggtgact
     8221 tgatcacatt ggactttggt tgctattacg aagggtacgt ttccgatatg acacgtacat
     8281 tcgctattgg tgatcctggg gaaaaattaa aagaaattta tcaaatagtc ttagatgctc
     8341 aattagcagt tattgatgct gcaaaaccag gaatgactgg tgtccaatta gatgcagttg
     8401 ctagagatta tatttcaaaa catggttacg gggaagcatt tggacattct acaggacatg
     8461 ggatcggttt agagatccac gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg
     8521 taccaggaaa tgtgatcact gatgaaccag ggatctactt accaggaatc ggtggagtac
     8581 gtattgagga tgacttgttg atcacagaag atggaaatcg tgtgttgact cgttcaccaa
     8641 aagaattgat catcttatag tttcatcaat agaagaagta agcgtaaaaa tcagtgttca
     8701 cagactttgt tatagaaaat cactcgattt aatgatacac tatagaagaa cttttaagct
     8761 gtaagagata ataaagttgg gtgttttctc caattcatag cagtttgcag ctgatcggcg
     8821 gcagttttct taccacgtga tcactatgcc gcatcataga gaattcggat gtatctcgtg
     8881 cttaggcaaa gggaggaata aataaatggc agatgaaaaa aatttgattt taaacgcaaa
     8941 ccaagattta ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc
     9001 agcttctaaa gtagaaggcg tatacggaat gcgcggaact tttgccagca atgtcactga
     9061 attattagga cgtgcagctc acggaaaagg tgtttactta gcaaacgagg aagatggctt
     9121 gaaagtagat ctttactgtt atctagaata tggtgtttca gttcctaaag tagcaatgga
     9181 aatgcaggaa cgggtcagcc agcaagtcct tttcatgacg gatatcgatt tatctgaagt
     9241 caatatccat gtagtagctg ttgttcctga aaaattagaa caaccaacaa tcgaagaact
     9301 ttttgaagac gaagaggaag agaatgaata aagaattatc aagacacgag attcgggaaa
     9361 tggcattgca ggctttattt ccattagact tcaatgcaga cctaacaaaa gaagatgcga
     9421 ttttcaacgc aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg
     9481 ttcctgttta tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat gaattggata
     9541 aagtgattga aaaacattta aaaaacaatt ggcacatcaa tcggatctca aaaatggatt
     9601 tggtgatctt gcgtatcgcg atttttgaaa tgacttatgt gacagatgta ccagcggctg
     9661 ttgctttaaa tgaagcaatc gagttggcaa aaacatttag tgatgaccgt tcacgtaaat
     9721 tcgtcaacgg tgttttatcc aatgtattga aagaattaga agctggggcg taagtcctag
     9781 ct
//
LOCUS       DAZUKD010000107         9718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000107 DAZUKD010000000
VERSION     DAZUKD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.109"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..875
                     /locus_tag="ACG8N9_002260"
     CDS             3..875
                     /locus_tag="ACG8N9_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4897820.1"
                     /translation="MVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKSTTI
                     RTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFMKLH
                     GKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSGLDP
                     LMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELRHLT
                     RSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKFESI
                     PPTLEDLFMRHYEG"
     gene            888..2498
                     /locus_tag="ACG8N9_002261"
     CDS             888..2498
                     /locus_tag="ACG8N9_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897821.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2575..3234)
                     /locus_tag="ACG8N9_002262"
     CDS             complement(2575..3234)
                     /locus_tag="ACG8N9_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4897822.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3517..4182)
                     /locus_tag="ACG8N9_002263"
     CDS             complement(3517..4182)
                     /locus_tag="ACG8N9_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4897823.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4329..4934)
                     /gene="dhaL"
                     /locus_tag="ACG8N9_002264"
     CDS             complement(4329..4934)
                     /gene="dhaL"
                     /locus_tag="ACG8N9_002264"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4897824.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4962..5972)
                     /gene="dhaK"
                     /locus_tag="ACG8N9_002265"
     CDS             complement(4962..5972)
                     /gene="dhaK"
                     /locus_tag="ACG8N9_002265"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4897825.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5947..6342)
                     /gene="dhaM"
                     /locus_tag="ACG8N9_002266"
     CDS             complement(5947..6342)
                     /gene="dhaM"
                     /locus_tag="ACG8N9_002266"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4897826.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6496..6816
                     /locus_tag="ACG8N9_002267"
     CDS             6496..6816
                     /locus_tag="ACG8N9_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4897827.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6928..7785)
                     /locus_tag="ACG8N9_002268"
     CDS             complement(6928..7785)
                     /locus_tag="ACG8N9_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4897828.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7893..9197)
                     /locus_tag="ACG8N9_002269"
     CDS             complement(7893..9197)
                     /locus_tag="ACG8N9_002269"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4897829.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9194..9703)
                     /locus_tag="ACG8N9_002270"
     CDS             complement(9194..9703)
                     /locus_tag="ACG8N9_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897830.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
BASE COUNT         2622 a         2045 c         1809 g         3242 t
ORIGIN      
        1 aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg
       61 tctctttcac cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa
      121 aatccacgac gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga
      181 tttttgggca agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc
      241 caggcgatat ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga
      301 agcttcatgg aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg
      361 atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg
      421 ctgctttggc tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc
      481 ctttgatgga aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca
      541 tcctactttc ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca
      601 tacgcgaagg aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt
      661 cgaccatcac tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc
      721 atgactttgt gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg
      781 tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc
      841 ttgaggattt attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat
      901 tttcacgctg gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga
      961 tatggctagt cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga
     1021 cttccggaca aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa
     1081 tggtcggacc aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa
     1141 atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga
     1201 gccaaacgag aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg
     1261 gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag
     1321 caatatcgat cagctgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg
     1381 cttggctttt tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt
     1441 ttgctgcaca actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag
     1501 gtggacttta ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga
     1561 atcctttagg ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc
     1621 tttatttgtt tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc
     1681 gagatttgaa caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc
     1741 ttttatcctt gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta
     1801 tcacttttgg tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt
     1861 tagaaagtaa cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa
     1921 actttacaag cacgatcatg gtagtcttga tttgtctagc tgctatttta ccaattgcta
     1981 gcatcaatcg tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa
     2041 aaatttctag gaaccaactt tattggacca atatgttact agctattcta tttgctatat
     2101 tggcaatcct gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg
     2161 cgaaagacat ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt
     2221 tagtgtttat cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg
     2281 cttatatcta cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac
     2341 cagaatggtt ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt
     2401 ttgatctttc cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt
     2461 ctattggtta taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca
     2521 tgctagcctc caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa
     2581 gtcttctaac tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc
     2641 attctcctct aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc
     2701 agcaagttct ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa
     2761 gtcaagcaga tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg
     2821 ctgctgtact tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg
     2881 ataatgcagt aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc
     2941 tagtgcttcg ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata
     3001 tccgcccggc tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac
     3061 tttcatactg ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg
     3121 ttctcctttt tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg
     3181 tactaagtga ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc
     3241 tcctccttct aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata
     3301 attttctttt atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt
     3361 tctttttgtc ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca
     3421 attttttaaa tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag
     3481 taaggtttgt ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag
     3541 cttttgatat agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc
     3601 gatttcttgc ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt
     3661 tttcaactct tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc
     3721 aagttcatgt tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca
     3781 ctctttcagt atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct
     3841 tttataatgt tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag
     3901 acaggcattc tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa
     3961 attccagcca ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa
     4021 aagtgcagct gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt
     4081 cttgatttgt tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat
     4141 tggtcgatca aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt
     4201 tatcttttgt attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg
     4261 ttctgcatca agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt
     4321 tattgaactt aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa
     4381 gcagctcctg ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt
     4441 ttagctacca agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc
     4501 cctgccttca atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct
     4561 tctgcctttc ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt
     4621 tcttgagaat caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat
     4681 ccgtataacg gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg
     4741 aatgtatcgc tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc
     4801 cctcttccca tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga
     4861 tattgtttgt tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga
     4921 acggttagtt tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt
     4981 acattgcttt ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa
     5041 agaccttgca tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg
     5101 cctctttctt ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc
     5161 gtcccgccca ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct
     5221 aaaatcttag taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga
     5281 tagcctggct caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat
     5341 cctggtttcc ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt
     5401 gcctgtacga ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct
     5461 gcatcgccca cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta
     5521 tacgtactat tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg
     5581 tccatagcag ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta
     5641 acgatcagca aaactccttt tccactatcg gcttccttaa tagccgtctg aatctgatct
     5701 ggagttggag aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca
     5761 aacccggcat gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc
     5821 gctttctgat tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag
     5881 ctttttacta atccttccag catttcttcg acaatattgc ttggatcatt catgattttt
     5941 ttcaatttat tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt
     6001 atcatctgtt ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc
     6061 caccagatga taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt
     6121 caataccgcg ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt
     6181 gactgcttct acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga
     6241 aaaaatttgc acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt
     6301 tactttttga ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt
     6361 tacatagttg ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa
     6421 tttctaattt tgggaaaata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa
     6481 gagaggagtg atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta
     6541 gcttataaag ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg
     6601 gaggcggggt tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg
     6661 ggtgcaagag aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat
     6721 gcgatcattg gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg
     6781 gtcacatgta gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac
     6841 taaagagaga tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag
     6901 gtactctacc ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc
     6961 tgcgactaac acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt
     7021 ttttggcaaa gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt
     7081 cgtgcttcca gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg
     7141 gaaactagct gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc
     7201 gctgatcttt ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag
     7261 ctttccagaa gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg
     7321 gatcttcccg tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt
     7381 tttctcaagt tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc
     7441 gattgctgct tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc
     7501 ttttctttta ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt
     7561 tattgatgta tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga
     7621 agtcatttct gtttccgagg caccgttttc agcaccagaa gagcttatgg ttgttgaagg
     7681 ttctgtttga gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact
     7741 ggaaacagca attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct
     7801 tactactttt taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca
     7861 acaggctgtt tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc
     7921 catgtcccgc ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc
     7981 gttatcgcgt tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc
     8041 aattttcttc gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc
     8101 atttcaaagc agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt
     8161 ccttgatata cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat
     8221 attgaccaga caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta
     8281 aaaggatgga gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc
     8341 caacgttttc tgtgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata
     8401 atctcaggag caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg
     8461 tcttctgtcg ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac
     8521 atcgtatttg ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta
     8581 atgatgccca cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga
     8641 ttttggacat ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa
     8701 taattcacat acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa
     8761 atcaatttcc cttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt
     8821 ttttcgatcc gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct
     8881 ggtgtttggt ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat
     8941 ttcgtcatca aatactcgat tgtatattcg atcacaattt cttcattatg ggctggcacc
     9001 atgatcgtga tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt
     9061 ttgtataaaa acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga
     9121 taagaaacga gaaaaaaata cataatagac aaacaagtat ctaagaataa attacctgta
     9181 tgggtgattg agatcatttc agttccaccc cattttcttt atacaaatct gcaatgaatg
     9241 tcgtatctag atttttctct tcggagacag aataaaaacg gaccgtttct ctctcttctt
     9301 ttgttccaaa acgttccgta tacacttctt caagtaattc ttgtcttttt ttcaattttt
     9361 catcttcata ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag
     9421 tcaatacaaa tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga
     9481 aatacagcat tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg
     9541 gtctcgttaa tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa
     9601 gagccactaa cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt
     9661 tcaccaaaat tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgt
//
LOCUS       DAZUKD010000108         9682 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000108 DAZUKD010000000
VERSION     DAZUKD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.110"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACG8N9_002271"
     CDS             complement(<1..195)
                     /locus_tag="ACG8N9_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897831.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACG8N9_002272"
     CDS             complement(211..825)
                     /locus_tag="ACG8N9_002272"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4897832.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACG8N9_002273"
     CDS             complement(972..1655)
                     /locus_tag="ACG8N9_002273"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4897833.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACG8N9_002274"
     CDS             complement(1764..2222)
                     /locus_tag="ACG8N9_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4897834.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACG8N9_002275"
     CDS             complement(2306..4069)
                     /locus_tag="ACG8N9_002275"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4897835.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACG8N9_002276"
     CDS             complement(4290..5750)
                     /locus_tag="ACG8N9_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897836.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8N9_002277"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8N9_002277"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4897837.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACG8N9_002278"
     CDS             complement(6907..8334)
                     /locus_tag="ACG8N9_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4897838.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACG8N9_002279"
     CDS             complement(8365..9579)
                     /locus_tag="ACG8N9_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4897839.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2867 a         2025 c         1610 g         3180 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tattttaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca at
//
LOCUS       DAZUKD010000109         9617 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000109 DAZUKD010000000
VERSION     DAZUKD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.111"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(512..1054)
                     /locus_tag="ACG8N9_002280"
     CDS             complement(512..1054)
                     /locus_tag="ACG8N9_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS4897840.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(1367..1918)
                     /locus_tag="ACG8N9_002281"
     CDS             complement(1367..1918)
                     /locus_tag="ACG8N9_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4897841.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            2235..2633
                     /gene="merR"
                     /locus_tag="ACG8N9_002282"
     CDS             2235..2633
                     /gene="merR"
                     /locus_tag="ACG8N9_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFS4897842.1"
                     /translation="MAYFISSFSEKCGVNKESIRYYERKNLLPEPDRTNSGYRVYSEE
                     DVKRVKFIKHLQEIGFSLNEIYKLLGVVDKDNIRCEGMFEFVSKKEREIQKKIENLNR
                     IEYMLKDLRARCPDEKELYACPIIEVLIEN"
     gene            2647..4287
                     /gene="merA"
                     /locus_tag="ACG8N9_002283"
     CDS             2647..4287
                     /gene="merA"
                     /locus_tag="ACG8N9_002283"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFS4897843.1"
                     /translation="MNQFRMRIQGMTCTGCEKHIEKALESMGALNAKADFSQSEAVFQ
                     LPDDYAIENAIEAVKKINYQPEEVEKLPVQKRVMTNNKEDYDLLIIGSGGAAFSAAIK
                     AVEYGSKVGMIERGTVGGTCVNIGCIPSKTLLRAGEINQSAHVNIFEGLHTSTGEVEL
                     DRLVDQKNELVDDLRKQKYTNLIDDYGFDLIKGEAKFIDENTVTVSGQTYSAQRFLIA
                     TGASPLLPRITGLKEVDYLTSTTLLELRKVPKRLTIIGSGYIGRELGQLFHNLGSEVT
                     LIQRSKRLLKEYDPEISEAMEKALIEQGIKLISGATYERVEQDGNIKKVHIIVNGKNK
                     VIESDELLVGTGRKPNTEVLNLSAAGVQVGPNNEIKINAFAQTSNRKIYAAGDVTLGP
                     QFVYVAAYEGGIAAGNAIGGLNKAIDLSVVPGVSFTSPQFATVGLTQQQAKEKGYEVK
                     KAVLSLENVPRAIVNHNTTGVFKLVVDRKTQKILGVHIVSENAGEVIYGASLAVKFGL
                     TVADLKDTLAPYLTMSEGLKLAALAFDKDISKLSCCAG"
     gene            complement(4812..5297)
                     /gene="vanZ-A"
                     /locus_tag="ACG8N9_002284"
     CDS             complement(4812..5297)
                     /gene="vanZ-A"
                     /locus_tag="ACG8N9_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS4897844.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5450..6361)
                     /locus_tag="ACG8N9_002285"
     CDS             complement(5450..6361)
                     /locus_tag="ACG8N9_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS4897845.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            6515..6667
                     /locus_tag="ACG8N9_002286"
                     /pseudo
     CDS             6515..6667
                     /locus_tag="ACG8N9_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(6787..7395)
                     /locus_tag="ACG8N9_002287"
     CDS             complement(6787..7395)
                     /locus_tag="ACG8N9_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS4897846.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(7401..8432)
                     /gene="vanA"
                     /locus_tag="ACG8N9_002288"
     CDS             complement(7401..8432)
                     /gene="vanA"
                     /locus_tag="ACG8N9_002288"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4897847.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(8425..9393)
                     /locus_tag="ACG8N9_002289"
     CDS             complement(8425..9393)
                     /locus_tag="ACG8N9_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4897848.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2974 a         1894 c         1752 g         2997 t
ORIGIN      
        1 taaaaattcc tcctggtatg ttttcttcta gtctacacac cttaattttt ctgtctagtt
       61 aattgtagcc tatccaactt aagattaatg acactaaatt aacagactat acgtagtatc
      121 tatttgctat gaccatatct tatatccata agtcaactca gccggactaa aagggctaat
      181 cttactagaa cacaagctgc acttgatatg caaattaacc ctgaatcgga aaggtgctta
      241 aattggatat taacggaggg ataaagtaaa atttatctct agggaatgtt tagaaaggat
      301 ccaagttcct taacaagtga caattagtaa tgtagtaaca acgatagatt gtatgccgaa
      361 catttcaatt tgatacgtat aaaagacgat ttcaagctca tccgtgataa ataacccaca
      421 tgaatgattt gaaatcgtct taaaatgctt tattttataa cccaaaatag aataattact
      481 tcaaaatgac gtcgcatatc actattaatg attaatcgat gacttccata atcattttaa
      541 gttccccgga tgttaaatcc tcgctaaaaa tgccagtaac tttttgtcca ggtccaattt
      601 cctttgatga taggtttaac aaagagatag ggatatagat taatccgttt tgtttcaaag
      661 acagagccaa tatttttttc cttgttcgaa tagctgcatc atagcgaaga gaagagtaaa
      721 cacctctgcc aacaatagaa atatgcttcc ccaataataa atcaaggcta cctattactt
      781 gtaatttctg aaaggtcgtc ccatccgttt tgatttgaat atcctgtaaa tttattttcc
      841 tgtctaaggc atcttgaaaa aacaagtgtg tgcctctgcg ataatcaatg ccacagaggt
      901 gcataaaatt agttgtcgaa aaaataatcg ctaccgttga gacttctccg acaaaggata
      961 cttttttatc tacaaaatgt tcttgtaaga gatgagcagc cttataaatc gcgtttaact
     1021 gtctggtaat ttgggttgtc tcttttatag acatgcgttt gtattctttt tttgccgcgt
     1081 gcgaagaatg ctcctttttc ccaataaaaa aagctgctca aaagagcagc cttttgtaca
     1141 ggtttagttt tggtgcttgc cacccgccgg acccttacgt tccggatttg tatcgagttt
     1201 agttttggtg ctcgccaccc gccggaccct tatgttccgg atttatatca agttgactat
     1261 gagcctgctc tcatttgtat atataatata ccaaaaaaag gttgatttgt ccaatactgt
     1321 gggtgattat aaccgtctta ctgtgcttat ctattatata atacaatcaa aaacctaacc
     1381 gaaaatttaa taacgtttca ctgatttcat cttcattaat tccgatgtag cgtttggtaa
     1441 tcttctcgct gctgtgacca aagatttcca ttaaggtggc cacgtctttg gttttcttgt
     1501 aatagtggta accaaaggtt tttcgtaggg tgtgggtgcc gatatcgtcc cttcctaaca
     1561 gcttcgcgac cttttgaaac atctggtaga ccgtattgac ttctaaatgg ccgcccttag
     1621 tactgggaaa caagtaagcc tccggatcta agggtgcggt atagccgtga atcaagtcct
     1681 gtaagctgct taagtataaa atgcgtgttt ttcctgtctt ttgctccacg atgcgcgggt
     1741 tttttgaggt aatcacgtct tttttcttga gtttaacgat gtcggacatg cgcagaccgc
     1801 tgttaattcc aattagaaac aagaaaacgt cacgttccgc atttttattc cgtcgcaggc
     1861 aaaataaaaa atcattaatc tcctgttgcg accgtagtgg ctgaacatta tagcccatga
     1921 caatccctcc tttttagtct gttcgaatac atgattatag ttattacaac acgaaatcgt
     1981 gtgtttaggg ggagtaaaaa aaaagaaccc tataaggata ggattcttga tacatgattt
     2041 tatgtaattt catgtattta ggtccgatat tatttattat aaaagaagtc tcaaaattta
     2101 ttggtaccac atttaatttt cacttcattt ttgaaagtaa ataagaagga aaatgatatt
     2161 ccccccttcg tattattttt ggttggacat gtacctaagt acatggttta taattaaaaa
     2221 aaggatgtga tagcatggct tattttatta gctccttctc tgaaaaatgt ggtgtaaata
     2281 aggaatccat tcgatattac gaacgaaaaa acttactacc agaacccgat cggactaatt
     2341 ccggttatcg agtttattca gaggaagatg taaaacgagt aaaattcatt aaacacctac
     2401 aagaaattgg cttttcttta aatgaaattt ataaattact tggtgttgta gataaagaca
     2461 acattcgttg tgaaggtatg tttgaatttg tctctaagaa agaaagagaa attcaaaaaa
     2521 aaatcgagaa tttgaataga atagaatata tgctaaaaga tttacgagca agatgtccag
     2581 atgaaaaaga actatacgcg tgtcctatca tagaagtatt aatagaaaat taacgggggt
     2641 aggaaaatga atcaatttag aatgcgcatt caaggaatga cctgtactgg ctgtgaaaaa
     2701 catatagaaa aagcgcttga gagcatggga gctctcaacg ctaaagcaga tttcagtcaa
     2761 agcgaagcgg tatttcaatt accagacgac tatgcaatag aaaacgcgat agaagcagtt
     2821 aaaaaaataa attatcagcc cgaagaagta gaaaagttac ctgttcaaaa acgcgtgatg
     2881 actaataaca aggaagatta tgatttgctc attatcggtt ctggtggagc agctttttca
     2941 gcagctataa aagcagtcga gtatggatca aaggtgggca tgattgaacg cggaactgta
     3001 ggcggaacct gtgtgaacat tggttgtatc ccttcaaaaa cgctgcttcg agctggagaa
     3061 atcaatcaat cagcacatgt aaatattttt gaaggattgc acacttctac gggagaagta
     3121 gaattagacc gcttagtcga tcaaaaaaat gaactagttg atgatttacg caaacaaaaa
     3181 tacactaatt taattgacga ctatggtttt gatttaatta aaggagaagc aaaattcatt
     3241 gacgaaaaca ctgtgacagt tagtgggcaa acatattctg cccaacggtt tttaatcgca
     3301 acgggtgctt ctccactctt acctcgtatt actggactta aagaagtaga ttatttaact
     3361 agcacaaccc tccttgaact aagaaaagta ccaaaacgat taacaattat tggatcgggt
     3421 tatattggaa gagaactggg acagctcttc cacaatctgg gttctgaggt tacccttatt
     3481 caaagaagca aacgactttt aaaagagtat gatccagaga tttctgaagc aatggagaaa
     3541 gctttaatcg aacaaggaat aaagctcatt tccggagcaa cttatgaacg agtagagcaa
     3601 gacggaaata ttaaaaaagt tcatattatt gtaaacggca aaaataaggt cattgagtca
     3661 gacgagctac tagttggaac aggaagaaaa ccgaatacag aagtattgaa cttgagcgca
     3721 gcaggcgttc aagttgggcc aaacaatgaa atcaagataa atgcttttgc tcagacaagt
     3781 aatagaaaaa tttatgcagc aggagacgtg acattaggac ctcagtttgt ctatgtagca
     3841 gcctatgaag gaggaattgc agcaggaaat gcaattggtg ggttaaataa agcaatcgat
     3901 ttatcggtag ttcctggagt atcatttacc agtccgcaat ttgcaactgt tgggttaaca
     3961 caacaacaag cgaaagaaaa aggatacgaa gtaaaaaaag cggtattgtc attagaaaat
     4021 gttcctagag cgattgtcaa tcataatacg acaggggtgt ttaaacttgt ggttgataga
     4081 aaaacacaga agattttggg agttcatatc gtttctgaaa atgcaggaga agttatttat
     4141 ggagcatcct tagccgttaa atttggattg accgtagctg atttaaaaga tactttagcg
     4201 ccttatctaa caatgtcaga aggcttaaag ttagcagctc tagcctttga taaagatatt
     4261 tcgaaattat cttgttgtgc aggttaatat ttctatttag tttcttgtag tagctggtaa
     4321 caccttggtt tatctttcgt tattatctat aacgatagga gttgaagatt atgacatcag
     4381 caaacgtcta cgtgccaatt aatagctcta ttctagtgga ggttgaagaa ataaaagact
     4441 atttagaaac gcaattgctc atttaggcaa cgcagcaaga tattattgaa gatgcgattg
     4501 ttgccttacg tgcttaatat agataattaa atcgtatcaa aaaaggattc taccgcttaa
     4561 aggggtaccg tcaggaaaat gcggatttac aacgttaagg aaatttatta aagagaccaa
     4621 ttcctcagca ctaaaattga taattgggct ttctttgtta ctatagtgtt aggtaagctt
     4681 tttactttat caccggtttt cccctcactt cacacctacg ggcgagtttc cccacatccc
     4741 gttttccatc taatccaatt cattcaatat ctttatgtgt atgaaagatc agattgcttg
     4801 atttagacat cttacacgta atttattctt aaatgggtac ggtaaacgag caataatacg
     4861 agcaaaagta tacctacaaa aataataact ctgtctaatt ttttttgatt catatgctta
     4921 ttgcttaaac catataattt cagtccaaga aagcctccaa cagtatttgt aattacatct
     4981 gttatgtctg tcgctccaat agcgaagata aattgaatta tttcaaaagt aagacttaaa
     5041 accagtacaa aagcaaactt aggtaaaaat ccgatttctt taaaattgac attcaaaagc
     5101 aagccaaatg gaataaagat tataacatta tctatcatct ctctgaaatt cccagtagca
     5161 gtaaatggag tcaagttaag acttctttga tgataattaa atactgataa aatattgtat
     5221 tgtaatttga ataacactaa ccagattagt gtcactaaat ataaagctag caatcctcta
     5281 gataatattt ttcccaatat aatttcctct aattcttttg tttaagttgt atcattagta
     5341 tagcaatgtc ctagttaacg tgggttttaa attaaaaagc taacgttacc ccaaaagtgc
     5401 tagtacaata atgctgttca ttaatttcgt tggtttcatt ccgccatcct tacctccttg
     5461 aattagtatg tgttgatccg ggaaacactg tcacaattac accgtctata ttgtttcctg
     5521 atatctcata acgaagatta gtcggcacat gaatggtggt atttttagta acaggataat
     5581 aaaagatctc atatttttcc ccatttacac taacagaaat ggttttttct tcttttaggt
     5641 aatccatata ttcctcgaga acgaaattct tttctttcat aatcgcacta tgtggtaaac
     5701 caacatagcg aatatgccat ggttcatatt gaattcctgt taactctgtt ttgtcctctg
     5761 gataacgtaa aatgaacccg tatttccaag cattttcttc tatccacttt ccttcagggg
     5821 ctcgttccat tttcgtcaag cttgatccta catctagtga taaacctgaa ttatgctcac
     5881 tataacctgc tggtaaggca tactcagccc ccatttcttg gtaaagcaca ctttgctcat
     5941 caaagtctcg atagccacta ttaataataa aatgactaac gccacccttt acagcatcat
     6001 tgaccatctc tgaaaatttt tgtgctattt cttttgacat ataaatatta ctatcaagca
     6061 acccgtatcc atttattaat tcgtcatgtt tagataaatt cacgatatct gacttcacac
     6121 tttcttggcg aacaggatat ttactattga ttaatagcag atttccttga taaacctgtt
     6181 cttctgtaat tgttttctct tgggtatttt cagaagtccc actattttct aaatgttctt
     6241 tgggattttg atcataattt tgaaattcga ctttttcctg agaaaacagt gcttcattaa
     6301 cgtagtcata acctaagtat attaagaata acaataacaa taaaaaaaac aacttcttca
     6361 ttttcagtct cctccttaac tatatagttg gaagtatgaa atatttctag aatcttcccc
     6421 ttaaacggca caatctcgtt tgagattgtg ccgttttgcc ccgtaaatta cagtcaatta
     6481 tttcgcccta ctggatttac tattatcacc aatttcctta gaaaaattag gttgtcaaaa
     6541 tttcaactca cgttctgtta aagggctaga gagtagattt gaaagcatat tactggctta
     6601 cagtgttcta ttaagtcgag cacaacgacg ttttgaagga actttgagat attctttagg
     6661 atattaaaat taactagcac cacgggttat ctactatata taaattccct aagaaccagt
     6721 tacatacgtc gggtttcctg ccgaaagagt tagcttatat agtttgtccg tgcaacggtt
     6781 aaaagtttat ttaacgggga aatcaaaata gctattgggg tatggttcgt ctcttaatac
     6841 atagtgccac cattcgaggc tatatgcttc aaacccactg ttttccatga tggagcgcaa
     6901 acgtctgcga ttttgcgctt cattgcatga tattccattt gccgcatgat gagagcgttc
     6961 atccataaaa tcaaatcggc tccccattgg tacaagctca cccgtgtcta atcgataaag
     7021 cgtaagatca atggcactgc cgcggctatg gcttgatttt gaagccacgt atccttttga
     7081 aatcatctca gttcggtcaa tattgggata ataactttcc tttgtcaggt tattttccgg
     7141 ctgtgcagcc cattgcataa aacagtttac agcacgctta ggacggtaac cgtcccatag
     7201 aagcaatccg tacccttggg tagcagccag ttcttttgcc ttcaaaagcg attcagccaa
     7261 ctcgtatgtc cctacaatgc gatttacttc ataaccgtca accggttttc cggtgaaatt
     7321 atcccaagtg gcatatttag cgtcccaacg aacaccgtgt actatttcat ctaaaaaagt
     7381 aaatcctatt tccatgctta tcaccccttt aacgctaata cgatcaagcg gtcaatcagt
     7441 tcgggaagtg caatacctgc agcggccatc atacggggat aacgactgta tgacgtgaaa
     7501 ccgggcagag tattgacttc gttcagtaca atgcggccgt tatcttgtaa aaacatatcc
     7561 acacgggcta gacctctaca gccgagcgct ttatatattt tttttgccgt ttcctgtatc
     7621 cgtcctcgct cctctgctga aaggtctgcg ggaacggtta taactgcgtt ttcagagcct
     7681 ttttccggct cgacttcctg atgaatacga aagattccgt actgcagcct gatttggtcc
     7741 acctcgccaa caactaacgc ggcactgttt cccaataccg cacaaccgac ctcacagccc
     7801 gaaacagcct gctcaattaa gattttgctg tcatattgtc ttgccgattc aattgcgtag
     7861 tccaattcgt ccgcgctatt gacttttttc acaccgaagg atgagcctga acgcgccggc
     7921 ttaacaaaaa caggataggt aaacgtagct gccaccggcc tatcatcttt attaataacc
     7981 caaaaggcgg gagtagctat cccagcattt ttcgcaacga tgtatgtcaa cgatttgtcc
     8041 atacaaattg ctgagctttg aatatcgcag cctacaaaag ggataccgga caattcaaac
     8101 agaccttgta tggatccatc ttcacctgac ttgccatgca aagctgaaaa tgctacatca
     8161 acatggttga tttcatattc atggttcttt ttaacaagta atccgtgcat ttttttatcc
     8221 ggcgagagta cagctgaata gcaattgtcg ttttcccatt ccgcgcaagg tttttcgcac
     8281 attttccata caccagattt cgtaattcca atgtataacg gctcgtattt ttctttatta
     8341 atgttagcgg ctatctctat tgcagatttt accgatacgt catgctcctc tgagcaaccc
     8401 ccaaacagta ttgcaacttt tattctattc atgctcctgt ctcctttcaa aatccaaaca
     8461 gtttttaatg gttttttcaa cggtatcacg caacgcttgc tcggtataat aggccgtatg
     8521 cggtgtgatt atcacgttag gcattctttg aagtttaagt aaaaattgat tatcaattgg
     8581 tttttgggtg caatcagagt agaaaaactc ttcctctcct tccaatacat ccaatgcggc
     8641 accgcccagt ttcccgtttt ctaatgcttt aaccaactca taggtatcta caagtggacc
     8701 gcgcccagta ttgataagaa atgctccttg cttcattctc tgtatttgtt cgtggctgat
     8761 aatatagtgc gtatccgtat tgagcggcac atgaagcgta acgatatcgc tattttgcag
     8821 caactcatca aacggtacat agtttacctc tatacttcgg ctgcgactat aagccaacac
     8881 tttacatcca aatcctcgca gccgctcaat aaccgctttg cctatctggc ccgttcccac
     8941 cacaccaact gtcatgtcgc tgagtacctt gccacggtcg ctgtccaacc tgaaatcatg
     9001 tttttccaca gagcgcacaa tcgattttac gttgcgtact gccataagaa ttagcatcat
     9061 agtataatcg gcaacgctat ccggcgagta cgccacattg tcgacagtga tgcccattct
     9121 cttagcagca gttgtatcta tatgattgca gccgatgctt cgggtagaaa tatatttcac
     9181 accggctctc ttcagcgcaa gaagaataga ggcggaaatc tctgatttat gtcccacact
     9241 gatacattga ttgaaaggcg cggatttggc gttggattcc gacacgttgg cgttaattat
     9301 cgttgccata acgccaaagc gaggcgaaag agcatggaat gcatctgcct catcctgctc
     9361 acatccataa acagtaatgc cgatgttatt cataattaag accaaccctt tctgtgaaag
     9421 gcaccaaata aaacggggat gccaatggta atcttgtcaa atctttctct gaagaacgaa
     9481 aacggctcgt tcttacggta ttaagtctac cgtgtaagaa cgagccgttt cttaatatta
     9541 aattgttaat ttcctaagaa aaagtaaaga taaccttgag attttcctaa aaaattatat
     9601 acatctctta ggacctc
//
LOCUS       DAZUKD010000110         9462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000110 DAZUKD010000000
VERSION     DAZUKD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.112"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..919
                     /gene="gnd"
                     /locus_tag="ACG8N9_002290"
     CDS             17..919
                     /gene="gnd"
                     /locus_tag="ACG8N9_002290"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4897849.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            921..1922
                     /locus_tag="ACG8N9_002291"
     CDS             921..1922
                     /locus_tag="ACG8N9_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4897850.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1919..2203
                     /locus_tag="ACG8N9_002292"
     CDS             1919..2203
                     /locus_tag="ACG8N9_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4897851.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2236..3033
                     /locus_tag="ACG8N9_002293"
     CDS             2236..3033
                     /locus_tag="ACG8N9_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4897852.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3047..3880
                     /locus_tag="ACG8N9_002294"
     CDS             3047..3880
                     /locus_tag="ACG8N9_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4897853.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3898..4755
                     /locus_tag="ACG8N9_002295"
     CDS             3898..4755
                     /locus_tag="ACG8N9_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4897854.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4777..5286
                     /locus_tag="ACG8N9_002296"
     CDS             4777..5286
                     /locus_tag="ACG8N9_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4897855.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5323..5724
                     /locus_tag="ACG8N9_002297"
     CDS             5323..5724
                     /locus_tag="ACG8N9_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897856.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5836..6189
                     /locus_tag="ACG8N9_002298"
     CDS             5836..6189
                     /locus_tag="ACG8N9_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897857.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVEADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6223..6666
                     /locus_tag="ACG8N9_002299"
     CDS             6223..6666
                     /locus_tag="ACG8N9_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897858.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6683..7768
                     /gene="uxuA"
                     /locus_tag="ACG8N9_002300"
     CDS             6683..7768
                     /gene="uxuA"
                     /locus_tag="ACG8N9_002300"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4897859.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7761..8474
                     /locus_tag="ACG8N9_002301"
     CDS             7761..8474
                     /locus_tag="ACG8N9_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4897860.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8681..8830)
                     /locus_tag="ACG8N9_002302"
     CDS             complement(8681..8830)
                     /locus_tag="ACG8N9_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897861.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9226..9426
                     /locus_tag="ACG8N9_002303"
     CDS             9226..9426
                     /locus_tag="ACG8N9_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4897862.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3178 a         1493 c         2102 g         2689 t
ORIGIN      
        1 aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg gattgaattt
       61 agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt caagtacagc
      121 tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg aatttctagc
      181 tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga taaccgaaaa
      241 catgttaaga aaactttttc caaaactcgg accaggagac attattattg aaggcggaaa
      301 ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg aaatcggtta
      361 cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct gcttaatgat
      421 tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg tagcaattga
      481 aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa tgatccataa
      541 tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag
      601 tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg
      661 cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt taagtaatta
      721 tcggggaatc gtttctgcat caggtgaagc aaaacggacg gttgaaacag cgctagaaat
      781 ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt cacaggaaga
      841 agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg gacatgaaat
      901 agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat tccagcagtt
      961 tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac gttcatttct
     1021 ttactgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa ggattcaata
     1081 tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac aaagatgaac
     1141 tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa gcagcaaaag
     1201 aacaccagac aatcgtatct atcatacctg ctttagttga acgaaatgct gtagcagaaa
     1261 ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa gcaagagtca
     1321 aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt aacaaaacag
     1381 ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg cgtaacggtt
     1441 ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc gaaagctttg
     1501 gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt tgtttatgtg
     1561 cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat cagatgaaag
     1621 aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa gcgatgatta
     1681 atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa gaagaaccgg
     1741 gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca catatatcgg
     1801 cctacacaag ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga
     1861 tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat ataaaaggat
     1921 gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt atttatatga
     1981 agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga aaagagtact
     2041 ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat ttttgaatga
     2101 cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg ctgaagccag
     2161 acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt tggcggagga
     2221 aaactgaccg atctagtggg ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc
     2281 cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta taaggagaat
     2341 ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt tataacagat
     2401 ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc agatacacta
     2461 gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga tgaatcattt
     2521 ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa tcattctgct
     2581 aaagcaatcc aagatatggc acaaagaaaa ctgacatcgg agttcaaaca gctttcagag
     2641 attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc aagaaatgtg
     2701 gcagcacatg ctatgcatga cgctatcagt aaatatattc cggaaagcca tcgttttcta
     2761 catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg tcgctgggaa
     2821 aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc tctagaacaa
     2881 atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt tattgattcg
     2941 aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat acagtcaatg
     3001 atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg aaacattaag
     3061 tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc gctggttagg
     3121 tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag tcgtcgggtt
     3181 gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa tgatttatat
     3241 gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag cagctatcgc
     3301 agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg ttgcggtgcc
     3361 agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata cttttttagg
     3421 taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc gatcaattat
     3481 tctttatcca acggttgcct catttttatt attcgttccg cttgttttca ttgctttata
     3541 tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag tcgttatcca
     3601 cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt
     3661 tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga gtgttgtttt
     3721 gaaatcttta gatattacga tggttactta tgccattttt gggattatta tcgctttgat
     3781 ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata acaattcagc
     3841 atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga atgggaaatg
     3901 gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca
     3961 aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga acgttacttg
     4021 gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa agatgaagac
     4081 tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt aagctggggt
     4141 ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga agaagagtat
     4201 gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc gggattgatg
     4261 ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata tatttttatc
     4321 gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt tccttttatc
     4381 tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc tttcactatg
     4441 ggccgtaatg ctacaggcct aaagcaaaac actggtgttc agacagcgat tgaaatgcta
     4501 tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa agtagaatct
     4561 tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct tgatcaaatc
     4621 gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa
     4681 ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt gctagcagga
     4741 atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt
     4801 gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa aagtggagtg
     4861 aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag agatctttac
     4921 aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga gaaagctaaa
     4981 ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc aaaaacagtc
     5041 caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa tattggaggg
     5101 attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta tttaaataaa
     5161 tcagatgctg aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa tatttatttt
     5221 caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa gtttgatttg
     5281 tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg
     5341 tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac aaaaaagagt
     5401 aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac agatgtgaca
     5461 gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg agatatcgaa
     5521 aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt aggaagttat
     5581 ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt attgtccttc
     5641 attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa tcctcatcac
     5701 gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg gagctacttt
     5761 ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat tcgaaatact
     5821 tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag tgtagcgtat
     5881 gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag atcgggtatg
     5941 aaaaaagagg ctattctttt ttatctagac tctgttgagg cagatacacc gctacagttt
     6001 tcgggaaatt atttttcaaa taaagaacag tggctgaaac aagttgataa gttgaaagaa
     6061 aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc
     6121 aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat tattgctctt
     6181 tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga aatcatgatc
     6241 gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca
     6301 tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac attcggagag
     6361 aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt atttgttgat
     6421 ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact gactgaagaa
     6481 aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt agaagcaatc
     6541 aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt caaacaagga
     6601 aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga tgaggacgat
     6661 ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg aaaaagggga
     6721 ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa ttggaacttt
     6781 gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt tgaaagcatc
     6841 tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc acgatgcaat
     6901 caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa caatcaaaaa
     6961 cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt ttggatgggc
     7021 gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat acgatcagaa
     7081 aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc agtcaaaggg
     7141 attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact
     7201 gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt tagaaaaagt
     7261 gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg atccgccatg
     7321 ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga aaattctaaa
     7381 tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag gggcaaatcc
     7441 ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt ttgtccactt
     7501 cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc cctctacaga
     7561 aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt tcgacggtcc
     7621 tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct
     7681 gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga taagtaagga
     7741 aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat atggatggcc
     7801 ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca caaaagtatg
     7861 attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa atggcgataa
     7921 gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg cgagaagcaa
     7981 tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg aaaaaaggag
     8041 cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta
     8101 atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc
     8161 aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc tttttgaaag
     8221 cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac tcgattgcag
     8281 ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat catttgatag
     8341 ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta tatgaatcca
     8401 ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta gaaagtaaag
     8461 ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg tcctgcacga
     8521 ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc ttatttgtag
     8581 tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag atgcgtttaa
     8641 agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat ttttttaatt
     8701 cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa
     8761 aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat
     8821 agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga
     8881 taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt
     8941 taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc
     9001 ggttattcaa ctttttcttc gattcgaaga caatagaaga caatgaattt tatatttcat
     9061 tgcattatcc ttgtgttatt agaaaaagca tgatatatta aatgagtaat ctattttgaa
     9121 acgtaatttg agcgatatat ctacatagat aaaactcctt ttgcctatca aatatttatt
     9181 gcaacgaaaa aacgtacatt gatacgtatc aggaggaatt tatttatgaa taacggtaca
     9241 gtaaaatggt tcaacgcaga caaaggtttt ggctttatca ctggtgaaga tggaaatgat
     9301 gtatttgcac atttttcagc tatccaaggt gacggcttca aaactttaga agaaggccaa
     9361 gcagtgtctt acgatgttga agatggtcaa cgtggccctc aagcaacaaa cattgttaaa
     9421 gcttaataaa aattgacttt taaccgtctt ttcgaaagaa aa
//
LOCUS       DAZUKD010000111         9459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000111 DAZUKD010000000
VERSION     DAZUKD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.113"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..128
                     /locus_tag="ACG8N9_002304"
     CDS             <1..128
                     /locus_tag="ACG8N9_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4897863.1"
                     /translation="RLLMEKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            191..1540
                     /locus_tag="ACG8N9_002305"
     CDS             191..1540
                     /locus_tag="ACG8N9_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4897864.1"
                     /translation="MIIRAINFYSLLPSTFDSIFFKLIGISGVLLLGIDFIIKFNKRK
                     VTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWAILQYFLIYQFAFENSDNKRFFK
                     RISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHRIRIGFLESRLFGTFSDINNAAI
                     ASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIYIVLSGSRSTYVVSIIIVAISSF
                     IVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNATKILFQELLPHLKEINFFNFTE
                     RHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNARITIWKSAWEIFKTSWLIGVSP
                     KNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVLNVYRII
                     YNKCIGKDYHFVYCLVILSYALYAALNNELVYENTVGTFVFWLFLGRVNSKNNSSYF"
     gene            1573..2520
                     /locus_tag="ACG8N9_002306"
     CDS             1573..2520
                     /locus_tag="ACG8N9_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4897865.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(2560..3720)
                     /locus_tag="ACG8N9_002307"
     CDS             complement(2560..3720)
                     /locus_tag="ACG8N9_002307"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4897866.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KELNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            3885..5060
                     /gene="mltG"
                     /locus_tag="ACG8N9_002308"
     CDS             3885..5060
                     /gene="mltG"
                     /locus_tag="ACG8N9_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4897867.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5196..5675
                     /gene="greA"
                     /locus_tag="ACG8N9_002309"
     CDS             5196..5675
                     /gene="greA"
                     /locus_tag="ACG8N9_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4897868.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            5843..6574
                     /gene="liaF"
                     /locus_tag="ACG8N9_002310"
     CDS             5843..6574
                     /gene="liaF"
                     /locus_tag="ACG8N9_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4897869.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAV
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            6571..7638
                     /gene="liaS"
                     /locus_tag="ACG8N9_002311"
     CDS             6571..7638
                     /gene="liaS"
                     /locus_tag="ACG8N9_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4897870.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            7645..8277
                     /locus_tag="ACG8N9_002312"
     CDS             7645..8277
                     /locus_tag="ACG8N9_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4897871.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            8417..9082
                     /locus_tag="ACG8N9_002313"
     CDS             8417..9082
                     /locus_tag="ACG8N9_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4897872.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            9110..9403
                     /locus_tag="ACG8N9_002314"
     CDS             9110..9403
                     /locus_tag="ACG8N9_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4897873.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         3196 a         1409 c         1919 g         2935 t
ORIGIN      
        1 atcgtttact tatggaaaaa tatcaaaaat tctctttaga aagagctaag gattttaatg
       61 aaaaaaaaat aattggtgaa tggcaaacat tgttaaatga attaggaaat gaggatgtta
      121 gaaattgagg acttcgatta aaaattttaa tttatggaaa gaatggtatc tatatatatt
      181 ttttataggg atgattattc gtgcaataaa tttttatagt ttattaccgt ccacttttga
      241 tagtattttt tttaaactta taggaatttc aggagttttg ctattaggga tagatttcat
      301 aattaaattt aataaaagaa aagtaactta taacatttta ttgatagcat atatggtgat
      361 tttagtaata acgagttttg ttcataaaga atatggtcta ggagaaaact taaagactat
      421 aatgtgggca atcttacagt attttttgat ttatcagttt gcttttgaga atagcgataa
      481 taaacgattt tttaaaagga taagttgggg atttatttat agttggtttg ttttagtatc
      541 aatgtcaatg gttcttttct ttggtaaatt cggctatgag aaatattata atgctagaca
      601 taggatcaga attggttttt tagaatcaag actgtttggg acgttttctg atattaacaa
      661 tgctgctata gcatctttag ttgttattgt cctaatctta ttttatcttt ataataaaaa
      721 agtgacagga ttaaataaaa tcattttttt aattaatctt ccattacaat ttatttatat
      781 agtattaagt gggtcacgat ctacctatgt ggtaagtata ataatagttg ccatatcatc
      841 ttttattgtt gtgttaaaac gaaatggaac aaaaaaagat ttaaaaaact atatccttgc
      901 tatagtatct tctattggtt ctgttctgat tttattcgct ttgtttaatg caactaagat
      961 actttttcaa gaattattac ctcatttaaa agaaataaat ttttttaatt ttacagagcg
     1021 tcataaagaa ttaatgaata atagtcaaca tattaataat actcaaacac ctgataatac
     1081 tcttgttagg aaagacgtag ctgaaaatac agatatttct aatgctagaa ttactatatg
     1141 gaaaagtgca tgggaaatat ttaaaacaag ttggcttatc ggagtctctc cgaaaaatgt
     1201 agtatcttat gcacagtcaa ttattccaga tggatatatt gcgaaagtag gaattgccat
     1261 tcataatgca tacttgaatg tattaacatc gacaggtatt ctaggaataa ttcctttcat
     1321 ggctttttta ataaagagtt ttgtgctgaa tgtttatcga atcatctata ataaatgcat
     1381 tggtaaagat tatcattttg tttactgttt agttatttta tcttatgcac tatacgcagc
     1441 tcttaacaat gaattagtct atgaaaacac tgtaggaacg tttgtttttt ggctattttt
     1501 aggaagagtt aatagcaaaa ataattctag ctatttttag taataaattt tatcttgaaa
     1561 agaggagaaa aaatgttgaa ttacttgatt accggtggcg ccggcttcat tggctcgacg
     1621 ctcgcgaatt atctaggaaa agaaaacaat gtagttgtga tcgatgattt atcgatgggg
     1681 aaaaaagaga atctggatga atcgaagcac atcacgttta ttgaagggga tgtcgctgac
     1741 aaaaatttga tggaaaaggt catgaaagaa taccatttcg attatatttt ccatctagct
     1801 gctgtagcta gtgtggcaga ttctgtagaa cgtcctctag aaactcatcg tgtaaatttt
     1861 gacagtgcgc ttttattgct tgaattggta cgaaaatatc aatctggttt aaaacgattg
     1921 gtatttagtt catcagctgc agtttatggc gatgaaccaa cgttgccaaa aaaagaagaa
     1981 tcggtcatcc gtcctttaac accatatgcc atcgataagt ttgcagcaga acaatatgtt
     2041 ttggattact gtcatttata tgatgttccg actagtgctg tccgattttt taacgtttat
     2101 ggtccaaatc aaaatcctaa ctctccatat agcggagtta tttcgattct agtggaccga
     2161 tacaaaaaac aattggcagg agaaaaaaca gaatttactt tatttggtga tggtagccaa
     2221 tctagagact tcgtttatat agaagatgtt atacaagcac ttttacttgt tgctaaggaa
     2281 gaaaaagctt taggacagca attcaatgtc ggtacaggaa aaagcactac gctacttgaa
     2341 ttgattcatt caattgatca aatcttagga acagagctag cgcttaaata tgaagcggag
     2401 cgttcagggg atattcgtga ttctttagcg gatatttcaa agatccgttc tttaggttat
     2461 caacccaaat ttgatatatt gaacggaatg gaacgttatt taaagacaga aatcgattga
     2521 aacaaaaaac cagcatcctg ctggtttttt tgtctcgact tagcttttta aattaacacc
     2581 ttccatcagt tcgtaaatct gatttcttaa atcagtggcc ggcaatttga tgtttttatc
     2641 agattggata ttactttcca tgacgattgc atcttttgtg tcttttttca tcgccgctac
     2701 tgcatggaat cgattgatat ttccctgagc aaggatataa tcatttccac tgtaaacacc
     2761 gccgctgtaa ggatgtttat caccattatt ttgccacaat gagaaaagta gttcttttgg
     2821 tataatatcg ccagacagaa cactggtaaa gaatttgttc aagtcataaa cagacatact
     2881 tatatttcct gtgcctaatt catttcggat gtctgtttca ggctcgacca atgcttttga
     2941 ataatcgtca gttgcagaca tttggtaaga tacagcatgc tgcggggaat tttttacatt
     3001 ttcataaaaa tctgtatgtt ctagatggag tggccgaatg atttcttttt ttaaaagagt
     3061 ttcataaggc tgattcatta gtttgatcaa tacccctgta agcaatgtat aatttaatgg
     3121 ttcataatga tacgtatcat aaccagtaaa tttcaaatga tgaagggcaa actgaatgat
     3181 ctcactatct gacataggtt tttttggaga agcggttcga tgaagaccag agcgcatgta
     3241 aagcatattt ttgattgaga tttgctgact tccaggaatc tgaggataaa attcagatag
     3301 ctttgtgtca aaagttatct tgccagattg aatttgtttt aggataagta aagcagtcat
     3361 cgctttttgg atcgaaccga tttgatagta aagagaagga gaattcaatt cttttatgcg
     3421 tagatttgcg tacccatatc ctttttctaa tatcactcga ttatttttgc gtacataaat
     3481 cgaaccgata taatctgatt tttgcagtaa ttgatcaatt ttgctggcaa gcatctgctc
     3541 attagatgtt aaattggaag caagttgtgc gggttccgtt ttataaattt gtggttgagg
     3601 ttgttttgtt aattgccatc ctgtaatgat gcctgttgaa aagaagctga cagccagcaa
     3661 caaaactgcg agaaaagaaa gagagatctg ctgttttttt tgatgttttc cttttgccat
     3721 attaaatcct tcctatccat ttttaactat ttttcattgt aatcctcaaa aagagcattt
     3781 gccacttaca attttgacat aatatttggt aaaataattc acggcagtag aaatttcatg
     3841 tataataaac agagttacga agatgatagt aaggaggagt tgttttgtca ggaaacaaac
     3901 aacacccaag caaagacaaa aaagaagcca gtaaggcagc tacagcagat ataatcgaca
     3961 agcggagaaa tctgcgtaaa aaagaagata agatcgtgag gaagatcatt ttagtgatcg
     4021 ctcttacatt attgattatt ggcggatttc tcgggtttac cgtatatcgt tatgtagata
     4081 gcgggttgaa gcctttagat aaatcggacg accaattagt ccaagtggaa atcccaagcg
     4141 gctcttctaa taagcagatt ggcgaaattt tggagaaaga caatattatt aaaagtggga
     4201 ttgttttcaa ttattataca aaatttaaaa acttaactgg ctttcaagct ggttattatc
     4261 agttagcgcc taatatgact cttgatgaga ttggcaagca gttgcaagaa ggcggcacat
     4321 ctgagccaac aaaagttgca gatgggaaaa ttgccattcc agaaggctac gacatcgatc
     4381 agattgctga gcgggtagcg aaggtaactg gtaaagacaa gaaagaattt ttagatttag
     4441 taaatgatga gactttcttt aacgagttgt atcaaaaatt cccagaattg ttggaaagtg
     4501 cctctaaagc tgagaacaca aaataccgtt tggaaggcta cttattccca gctacttatg
     4561 attactacac gagtacttcg cttaaggatc ttgtgattga gatggtgaac aaaacaaata
     4621 cggtcatgca aaactattat tcagctatca aacagaaaaa tttgactgta caagaagttt
     4681 taactttggc ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt aaaaatattg
     4741 cacaagtctt ctttaaccga atcaaggcaa atatgccact tcagtcagat atctctgttt
     4801 tatatgcatt aggagaacat aaagaattgg tgacgtatga agatacagct gtagattcac
     4861 catacaattt gtataccaat accggctatg ggcctggtcc ttttgacaat ccaagcgaag
     4921 aggcaatcaa ggcagtatta gaaccagcag aaaatgacta ttattatttc gtagcagata
     4981 cgagcactgg aaatgtttat ttcgctaaaa cgtatgaaga acatatggaa ctagtgcaaa
     5041 aatatgtaaa taattcttag aaaaacaggg gctagtcatt gatcattttt agaaaaacat
     5101 ttacatttta agaaaagcgt ggtaatcttt tttggactat attcctcaaa agattacctt
     5161 ttgcggtatt ttatcgaaaa aaggagacgt tacacatggt agagaaagtt tatccaatga
     5221 ctctagaagg taaagaaaaa ttagaaaaag aattagaaga actaaaaaca gttaaacgcg
     5281 gagaaatcat tgaacgaatc aaaatcgctc gcggtttcgg agatctttcc gaaaactccg
     5341 aatacgaatc agctaaagat gaacaagcat ttgttgaagg aagaatcaca acaattgaaa
     5401 atatgatccg ttttgcacaa atcatcgata atgacggcgt agatgcagat gaggtatcga
     5461 tcggtaaaac agttacgttc atcgagttgc ctgatggaga tgaagaagag tacacgattg
     5521 tcggaagtgc tgaagcagac ccgttttctg gaaagatttc caatgattct cctatcgcgc
     5581 aagcattgat tggcaaacgt aaaaatgatg aagtaaccat tgctacacca ggtggcgata
     5641 tgttagttaa aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc tagagagttc
     5701 atcttactga tgacgcactc tagatctttt ctctttgagt attgcaggtt tcactgaacg
     5761 atcttacatc taaagtccta tagccaacag tcaaaaaaat aggtatgatg aaatgagaat
     5821 gtgtcaggag ggagaaaaag cgatgaatag ttcttggcgt ttttttgtgg tagtcgaagc
     5881 actgctgctg ttatttgctg tttggcaaat cgtaaacaat actggacttt tgcttttagt
     5941 aatattcggt attttcaata tatatctagc tgtgcgcaaa tatcctcgta ctaaatttca
     6001 aaattttcaa ttgatcttag ggagcttggt catttttttt agcttagtca acagtcctgc
     6061 tttatggatg atggttgtat tggccgtttt atttatcggg ctgaaaggtg tagagatttc
     6121 aggaatagat ttgacaaaaa atgctttttg gcgaaaaaaa cagatcatga tggtccaaac
     6181 agagcaatta aaaacacata ataatgagcg taaaagacag caattatttg gtaaccaacg
     6241 tattggaaac gacgtctatg aatgggatga tatcaacatt gccatcattt ctggtgatac
     6301 tatcatcgat ttgggcaata cacttttgcc aaaagatgat aatattgtga ttgtaagaaa
     6361 agggataggc agaacacgga tcttagtacc cctgggagtg gctattcgtt tagagcatgc
     6421 gacattagta ggaaatgtct tgtttgaaga agagcagttt tccttaaaaa acgaacagat
     6481 caaaatctgc agcagtgatt atgatgaaaa tcctcgcaga ttgaagatca ttaccaatac
     6541 tttggttgga gatgtcgagg tgatacgaat atgatgggaa gaatatccaa agcaatgcta
     6601 gctgtttatt cggggattgc ggctttcctt attattctat tttcactttt cacttatttt
     6661 tatgccagta accaaagtca ttggtgggga gaattgctgc gtgcgcgttt attatacgtt
     6721 ccgcttatct tccatttgat ggcaatttca ttaggtgtag gattgatcgt atttctattg
     6781 ttatcactta ttcaaaaggc aaaatacggg aaaatcgaag aaaagcttcg tgcactttct
     6841 tctggcaatt atgaatccaa acttttgcat cagccgatcc caagcgcgtc ggatgatctg
     6901 tacatcaaag atattgatat ggaaattact aaaataaaag aaaaaatgat cgaagtatcc
     6961 agtgaattac agattatgac tagccgtcct caatatgtag atggacaaac caaagaagag
     7021 attttaaaat tagaaaggca tcgattagct cgtgagcttc atgattcagt ttcacagcaa
     7081 ttatttgcag cgatgatgat gatgtcggca ttgacggagc aagcagaaaa aagtgagaca
     7141 ccagaaatgt tccgtaagca gttgaaaatg gtggcagaga tcatcaacgc ttcccagtct
     7201 gagatgcgtg cgctgcttct tcatctgcgt ccagtcaatt tagaagaaaa aagtttaaaa
     7261 caaggcattg agcagttatt gaaggaattg cagaataaaa tccagatttc gctaaaatgg
     7321 gaggtagaag atgtaaaatt aactagttcc attgaggatc acttgttccg aatcgttcaa
     7381 gaattgttat caaacacatt aagacatgct aaggctaatg aattagaagt ctatttgcat
     7441 aaaatagata acaatctttt attacgtatc attgacgacg ggacaggatt taatatgaat
     7501 gaaaccaaaa cgggaagtta tggattgaac aatatcaaag aacgagtagc tggtattggt
     7561 ggaacagtaa aaatcattag ttttaaaggg caaggtacga gcgtggagac caaggtccct
     7621 ttgatgaagg aggcatagat aaagatgata aaagttttat tagtagatga ccatgaaatg
     7681 gtacgcttag gcgtctcttc ttatttatct atccaagaag acattgaagt gattggagaa
     7741 gctgaaaatg gacgtcaagg ctatgaaaag gcgatggcac ttcgaccgga tgtcatttta
     7801 atggatctag tcatggaaga aatggatggc atcgaatcaa caaaagcaat cttaaaagat
     7861 tggccaaaag ccaaaatcat tatcgtcaca agttttattg atgatgaaaa agtttatcct
     7921 gcaattgaag caggagcagc aggatatttg ttgaagacgt ctacagcaca tgaaatagct
     7981 gatgctataa gggctacgca acgcggcgag cgagtactag aacctgaagt gacaacaaaa
     8041 atgatggaaa aaatgagtcg gcgaaatgat ccagtattgc atgaagaatt aacgaatcga
     8101 gaaaatgaaa ttttgatgct gatttctgaa gggaaaagta atcaggaaat cgcggatgaa
     8161 ttgtttatca cactgaaaac agtcaaaacg catgtgtcga atatattagc taagttagag
     8221 gtagaagacc gaactcaagc agctatctat gcgtttaaac atggtttggt gaaataaaaa
     8281 cgatgataaa gaggataatc aaaagctgag aaaataacta ttatttttct tggctttttt
     8341 acaataatga tgcttgcttt tgtttttgga aaggctttgt tatactgaga aaagaagata
     8401 ctaaaggagg aaaatggtga aacaaaattt tgcaattgta ggccttggac gatttggcgg
     8461 aagcatctgc cgtacactga tcgaagctgg acaagaagta ctggctattg acagtaatga
     8521 agatcgagtc aatgaatata tgaatatagc cacccatgct gttgtggcaa atgcacagga
     8581 tgagatgaca cttcgttcat taggtataag aaatttcgat catgtaattg tcgctattgg
     8641 agaagatatc caagccagca tcttagttac tctgatggta aaggaaatgg gcgtaccgaa
     8701 tattttagca aaagcccaaa atgagtacca tgcccgtgta ctagagaaga tcggagcaga
     8761 tcgtgtcgta caccctgagc gagacatggg gatacggatt gcacataact tagtatcaaa
     8821 gaatattcta gactacttag agctgtcaga taaatattct ttagcagaaa tccgagtctc
     8881 caatcctaaa tttttcaaca aaacattagc tgaattaaat ttcaggcaac gttttgactt
     8941 aacagtagtc gccatacgaa gagtagatca aacggttatc gcttctcctg cagcagatga
     9001 atatgttcgt gaaaatgata atctactggt tattggagag acaggcgatg tggatattct
     9061 agacgataag atgaatcaat agaaagaaaa gagaaagtag gttgttggga tgaaattaac
     9121 ggtaacgcca aaagcacaaa agtggtttga ctcagaaatt gatctgccag aaacttacgg
     9181 aatacgtttt tttggaaaag tctacggcaa aacagaggtt catgatggtt tttcgattgg
     9241 aatgtcagtt gaacagccag aacatcctgt aaagaaagtg gaaatggatg ggacactttt
     9301 ttttatcgag gaagcagatg aatggttctt taaaggatat gatttacttg ttgattatga
     9361 tcccaacctg gaggagccaa cctatcactt taaaaaacaa taaaaaaaga gagggtgaca
     9421 caatttttgt caccttctct tttttctttt gaaaatatt
//
LOCUS       DAZUKD010000112         9402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000112 DAZUKD010000000
VERSION     DAZUKD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.114"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            510..1313
                     /locus_tag="ACG8N9_002315"
     CDS             510..1313
                     /locus_tag="ACG8N9_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4897874.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            1920..2375
                     /locus_tag="ACG8N9_002316"
     CDS             1920..2375
                     /locus_tag="ACG8N9_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897875.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGTKKGR"
     gene            2494..3552
                     /locus_tag="ACG8N9_002317"
     CDS             2494..3552
                     /locus_tag="ACG8N9_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS4897876.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3594..5543
                     /locus_tag="ACG8N9_002318"
     CDS             3594..5543
                     /locus_tag="ACG8N9_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4897877.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            5546..9235
                     /locus_tag="ACG8N9_002319"
     CDS             5546..9235
                     /locus_tag="ACG8N9_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFS4897878.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVGGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDAKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
BASE COUNT         3361 a         1393 c         1975 g         2673 t
ORIGIN      
        1 ctttttttca attattcaaa attacacaag caaaataaat tgctagatag tgttagtttt
       61 tgttttaagg tttttaattt ttgagatttt gaaataagag aggaggaatc ctcctttatt
      121 aaatatctgt taatatatct gttaaatatc tgttaatata tctgttaggg gggtcttaaa
      181 cccttgggag agtaagtatt gaaggggtat gaaggtaaca cttttggaca gtaaaggtaa
      241 caaaagtgga cagtaagggt aacatttttg gacagtaaag gtaacaaaag tggacatttg
      301 aggtatcaaa tcaggataag acgttacctt cggttttctt gatttggaaa tctaaaaagt
      361 agaaaactag attagtaaca aatctttgtt gactgttacc tttagttgtg atttaattta
      421 ggtaacaagc agaaaaaaag cgtttccttt aatttagtac caaattaaca tgattaaagg
      481 gatgcaacaa gaagattgga gagtaatcaa tgtctcaaga agttaccgaa aacgttattg
      541 ataatgatgt agatttagta tcagatcaaa caaatgcaat tgttcgttat aacaatggtt
      601 tgaacacaac agctttgcga ggatttaaac ctgtagaaat ggatgtgttt tgggctattt
      661 tatcaaaaat gaaacgtcaa ggaacagcag aagtaacttt tgatttcaac gtctttcgtt
      721 atttggtcaa atacgacaga agagaaaagg acagttttta ttctgtgtta aaagctttga
      781 gtgacaaact aggtacttta acctaccgct ttgaagattc agatgtctat gaacagctgt
      841 ggctttttca aaaattccga atagataaac agcaagaaac agttacaatc aaggcaaatg
      901 aggagtttga atttatctta aattcaattg gcaaaaactt tactcgtttt gaacttgata
      961 actttacaaa actaagaagt agctacacaa aagagctgta tcgtcatctc atgcaatata
     1021 gaaataaaga gtggcgaaat ggctattggg tagtcaaagt tgaagatttt agaagagcgt
     1081 taagtattcc ttcaagttac agaatgacaa atattgacaa aagaatatta gagcaggcga
     1141 aagaagaatt tttagctcct gatgaaaatg gtatacaggt atttgaatct tttgattatg
     1201 aaaaaatcta tgcgaagaaa ggaagaaaag ttgatcggct cagattcact tttagtgaac
     1261 cagaatcaaa cttgccatcc atttcaatgc ataattggtt agaagaagac tgaaaagaag
     1321 caggttgtaa ttttaataca acctgcttct tttgaaaaaa gtatgggtaa cacttttgga
     1381 caataaatgc aagaagtttt tttgtgacca aactttatat tagattactg tgattttgtg
     1441 tgttagaaaa gccctgatag aaaagcaaat tcaaacactt tttaatatgt atgggtaaca
     1501 cttttggaca tgtcaataat aaatatattt tctttcgtct tttgcctaac taaaggtaac
     1561 acttttggac agtatagttt tgtaaactaa ttagatcggg tttaatggga tatatcttag
     1621 catttatgta agaatgaagg taacattaat ggacatgtca actgtttgaa caaaaaaata
     1681 tagaatattt ggatctgatt tttcattttt ccaaacgtta aaaaatagtt agttatttta
     1741 ttaatagata gtcatcctta atgtttgaaa tagcagaaaa taagatattg tttgataaat
     1801 gttgttgtta taaggaaaac ttgaatttgt taattgattt atttatgtta ttatgatact
     1861 ataataacat aaatgtgtta cgcccgaacc tcattttaca aaaatgaagg ggtgagacaa
     1921 tggatgctat taattggcat gaatttgtta actatatgga gaaactacta gatgatctga
     1981 aaagttttag cattgctgat gccgtaaaac atgggttgcc agttagtaca acgatgaaaa
     2041 tgcgagggtt gaaggttccg gatgaaatta tcaagctgag gactaaaaca atcattgaaa
     2101 ctgcaaaaat tgttgacagt atgaaccttg atttgaaagg caaaaaaaca acttatgaga
     2161 aagactttgt acgtttttta aaaaatgtta tcggaaaata tctagggaaa aagtctttta
     2221 ctgaattgag agacgaagag attgtaccaa ctccaatggt tagcaaaatt cgcaaaaaca
     2281 aagccgaggg aaattactat cttggcacaa tagcaaaaat cgttgagtat ttggaaaata
     2341 aagagcaaaa gaaaggaacg aagaaaggaa gatagttcgt gtgataaata tgaataggga
     2401 agaatgtacc gaaatgataa aaaaacccga acctagaatt accgaaaaac tgactgattg
     2461 cttgctcttt tacgaaaggg ggcaggcaaa ctaatggaaa aagatgtcaa ttcagcatcc
     2521 actaaagatc aatacaatat cattggagga ttacttggtg ataaggataa gagaacacgt
     2581 catggattac gcattatgct tgcgacattt ctaggggtgt ttcttttatt aacaggcttg
     2641 ggtagctaca tttatcaatt aggaaactat aattattatc ttgtcaacaa tacaacacca
     2701 atcggctcat cattgacgtt tgctcgatca ggagcagaga tcactatctc agatgtttgg
     2761 acagataaaa ctagagatgt aacggtggtc aaacttggtt actcaggaaa cgcaagaaaa
     2821 ttgctatcta cacaaggtaa aaattataac ttgtatatgg caacaaaaga aaaagatcgt
     2881 cctaagaagc ttgaaatgag ttatgggatg ttaagtaccg atggtgatgc tttcttgttc
     2941 ctaaaaggta aattagatga aagagcttat caagttttta ttgctaacca agtatcacta
     3001 atttcaggtg atgatacagc tgatgatgat accaaagtca atgtgaatga atcagattca
     3061 gtcaccaagg cactttctca atattctatg aatgatgttg ataatgaagg agtaatttct
     3121 tttgggaaaa aagcaaaacc agttaatact cctgataata tcaatttcag aataaatcca
     3181 tactcagata caactaacgt atatcaagga agcttcctta ccaaagatgg cgatattgat
     3241 tactcaaaag ttgtagctgt gacaagtaag aataatgttg taaataagct agaagaccag
     3301 ttaaatgctt caaatactaa catcaagaaa atcgaagcca caaaagctga gtatcttgaa
     3361 cgtttacaac aaaatcctga cgataaagat tcgcaagaaa gtattcgtca actagacagt
     3421 cgaatggaga ccgaaattaa aacaatcaat gatacacaaa aattgattga tcagtacaaa
     3481 aatgctgact ttaacaaaga ttcttttgga gatatgaaga caacatttaa gtacctaacc
     3541 ctgactgact agaaacaaat tattcttatt ttagaggaag tgaacacaaa cagatggaaa
     3601 atagtaccga gttctttaaa aatattcaaa ccgaattcaa tcatgctctc acactaatga
     3661 aaccaccgaa acataagtta gtggggcgtg atgacgaact ccatgagttg tcactgatct
     3721 taaatagacc ggaaacacca caagctatct tgttaggtga agcaggaaca ggtaaaacag
     3781 cactggcaag tgaatgggct aaaagacagc gacaagaggg gaagaaagta caagtatata
     3841 aattatcaat cggacaattg cgagttggag gagatgacgt cctccaaaca cgtttggaaa
     3901 ctataattcc aagattgaag gaatacgaac gaattttaaa atccgaggac aaagataatg
     3961 aatttgtcct ctttatagat gaatttcata aagttgtcag ttcatttggg agcggtaata
     4021 aaattggggg agattcgctg aaggaacctt tagcaaatga atcacttcag ttgataaccg
     4081 ctacaactag aaaagaatat gaaaactata tcaaacgaga tgatgcttta ggtagacgac
     4141 tacaaccgtt agaactcaac gaattaaaaa gtgatgtcgt tgtcgatata ctaagacaat
     4201 ggttgaatac atcatctgtc aaagatgatc ctgaagcttc cttgaaatta tccaaaaaga
     4261 ttcctgatga attgcttgaa gaaatcgtac aagcaaacaa gctttatcgt gaggatttat
     4321 ccgaaccggc taaatcaatt gccgtaatgg aagatttgat tgcgaaggat cgtgtagatg
     4381 gcaatgcgta taatcgagaa agcctgttac aaatatttgg aaaatataat attcaactag
     4441 attctgattt tgatatagca aaattggaag aggagctaaa gtccattaaa gggcaaccag
     4501 tagcggtata tacggtcacg atgttgttta aacgaatgaa acttggattt gatcgtttta
     4561 aagggcgaaa gcaaccaaaa gcaacggctt tgtttacagg atcaacaggg gtcggtaaaa
     4621 ctgcgatggc taaagctgta gcaagagggc tatacggtga tgaaaataaa gtccttttag
     4681 taaatatgcc agactataaa actaaagatg atgctatgcg gtttagaaag cttatcggaa
     4741 cacatgtccg gcatgatcca tcagcaatca ttgtttgtga tgaatatgaa aaagcaggcg
     4801 tagaaacact tgatgccttt ttatctattc tagatgaagg aagaatcagt tttactgaaa
     4861 gaggatggga tgatcgagaa gttaatgata ccgtctcact ttcaaatacg attttatttg
     4921 cgacaacgaa cgcaggggct gagatgtacg attcgatgaa ccgatacagc tcactatctc
     4981 gtgttgctaa tcaggaaaat tcaaatgagt acagcactga gcttaaaaga gagtatcgaa
     5041 aggtagcatt agagattaaa caagctctaa ttggtgaagg tctaaaaccc gaagtcatca
     5101 accgatttgg acggattgtt ccattttggg ctttatcgga acaaactctt ttggaaattg
     5161 cacacaatga aatattgaaa agtgctgaat tggtaagaaa acacttaaac gtagacttac
     5221 gccttccacc acagaaaaaa catttctatg gtcaagaatt ttatgcagat gatctctcca
     5281 tgcacttagt ttttgataga gcttcacaag aagattctaa atcagggggt gcaagaaata
     5341 tcttgcatct ggtcgaatca gaatttgaag aagcaattac tgaagcaata attacctatc
     5401 ctaacgccaa aatatttgac gttaaattga gcaaagcagg agggtttgaa gggaaaggcg
     5461 atacggatgc agaaattatc gctaaacctt ccatcgctga aagtaaagaa aacaagtttg
     5521 tgttttcggt tggaggggtg tagctatgaa atttaaaaag tctttacttc aaaatcgtaa
     5581 aattaagcga tgggttgtaa gaggtttagt tatcgtgggg gcacttgcag ttactttagg
     5641 cgctccattt ggaatgaatg atccaacgtc tgatgatcaa ggaaataaac aagaaacagt
     5701 tgcaaatacg aatgtggctt atgctgaaag taaaccggca gaaaaagggg tatggacact
     5761 atatgctcgt atcctgatgg gagataaaga ggtcaatgat agtgccacta aaactggtca
     5821 aatgaattta cttggtcaag gaggtattag tgctgacttt ccttattctg atatttccaa
     5881 acaaggtaat gcaattgccg gtggtaacgc aggtacgcaa ttagcatcaa ccttagcgac
     5941 ttataattac tatgggtata tcgaaacagt ttcaggaaat gcgattgcaa gtcaggcatc
     6001 ctcttttatt ggtggtatcg gtagaacgct tggcagtttt atcggatcaa ttagcttgat
     6061 actttctaat attgctgaat ccatcaatga actccttgga aaatttttca tgagtatgaa
     6121 tatattttca cttatgggtt ggggtaaagg tggatcggat gtttctaatc cggtagctaa
     6181 ggcgttaact agcttactta ataatttggg attaaacaat caattattta aagcgatagc
     6241 aagcttatct atgctctttt ttacagcaat actaggctat caaggaatga aactgttgtc
     6301 gaaagagggt gttaactcaa agggattatc accagtactt gaaaaatgga ttgttcgtat
     6361 ttttgtattg tttgtttttc taccagttgc aggatcgtta ttgggagttt atatcaatgg
     6421 tattcagtca gcaaaagaag atggaaaatt accaacaaat gttgttggtg attacatcat
     6481 taatacaaga ctttgggctt ctactcaaaa tcttgctcca tcatccgata ctggtgggaa
     6541 tgtgccaaac ggaaaaccaa catctgaaat taaatttgtt gacactgact atgaaccatc
     6601 taaaaaatca gaaatgatca gaatgatcaa tactagcagt tacgctagac aaaataatga
     6661 tctagattcc aattcagtcg gcatggcatt gttaaaagaa tggtcaagta atgatacgtt
     6721 taatgtcaat acttatgccg gtgatgtgaa tgtagctgaa aatgatgctt atatgggaag
     6781 agcgcctgct gtttggacag gctataattt aggggatgga aaagaaggaa atgcaattaa
     6841 gaaacctacc aatttaaatg gttatatttg gtcagctaat caaaacgtta cagacgacaa
     6901 ccgcaagcct gatggaaaag gaacaacgaa taactttgat gctactcaac cttggggtgt
     6961 tgctaacaat gcaacgttta gcacacagtc ggtagcgttg atgctccaat cagcactgga
     7021 tacatcaggg gctaagtttt atgcgtataa tatcgctcca tcaggaattc aaggtagtgc
     7081 taagaatatg tcaacagtaa agacagaatg gcgtacagct tctttagttg gtcatggagc
     7141 tatgggtaaa ttcggctcat ggttagcgat gaacgctgaa tcaatcgtat tagcagtgct
     7201 ttatctagct tgtgcttggg cgatgtttaa cgttaatatt gtaaaagcct tcaaacgtga
     7261 gtttgtgcga gtttgggaag tagtcttaac agggtctctt gccgcaggag caggggttat
     7321 ggcaatctca ttaggagtcg taaccacatc tcttatcgga ttacaattac ctatttttct
     7381 gatcaccttt gttcgtgcat ttggagatac ggctgtagga ttcatcccta acacattact
     7441 tggtgatggt gtagcaaata ttgtacgatc cgctatggtc ttaggcgtag gataccttct
     7501 agtctttaag aaaggtgaaa aggttaataa taatttgatt aatatgctta ttggattacc
     7561 acttgaagta gcgtttggtt ttgacgaacg tgctaagtta ttatttggca atagaaatga
     7621 tgctgttcga gttgtggatg gcgtttctaa agcaatcaaa aaaagccaag gggctaaaaa
     7681 tccttattat gatggtggca gaggatcagt aaaagatatg gtcgcaggag gacttggagc
     7741 agtcggagcg caaggaattg ctgatcgttt gaacaatatg ggagacagca atgctgatgc
     7801 taagacaatc gctgataaag ttgttcctga aacgcctgct tcagtaccat ttaatggtgg
     7861 cggagttggt ggaacaatca ataatacctc tttcaaagat ttagttcgtc aagatcaatt
     7921 acgtaataac tcgcaacaat atagcgatca tctaggacgg atggttgatg aaaaaggaaa
     7981 ctttgtcgat gaaaatggtg aagtattaaa agacagtaaa tttggtaaag atgccaaagg
     8041 aaactttatt gatgccaaag ggcgtttggt cgatgaagat ggaaatttga tagatacaaa
     8101 cggtaaacca ttggaagaca atgaagatgg aaacatcggt atggatagcc aaggacgttt
     8161 aattgatgac aaagggcgct tggtcgatga cgaaggaaac cttgttgatg aaaacggacg
     8221 taaattactt aacggtgatt ttgggcagga tgtcaatggt cgtctagttg atgataaagg
     8281 acgattagtt gatgaagacg gaaatctggt cgatgagttt ggtaagaaac ttggaaaaca
     8341 cagtaacggt tataacgtaa atggcgatac agtcgatgaa gatggaaaca tcattgattc
     8401 aagagatgct cttgtagcag atcgaaataa agaactcgaa caacaacagc atacaccaat
     8461 tgcggttgca atccctgatg atgaaagcga tgaagttatt ttagatcaag cacatcaagg
     8521 gttaagagaa gaagcaaagg ataaagttgt ttcagatcaa gcacaagaag agctaaaaaa
     8581 taaatcgaat ggtgaagatg tttcaaacca atatcatcaa acggctggaa atgtacctgt
     8641 tgaaggtcag tctaatgagc ttcacaacaa ctttgataat gttgctgatc ggaatgatac
     8701 agtcttaaaa gaaaaagaat cattaagaca agaaacagat aaagaccaat caaaattgaa
     8761 gaaaacaact attcctactc ctattggttc aacagttccg aaggtcaatc aagctaacga
     8821 ttctgaaaca aacactgggt taggaatggc gaaagacaat caatccatta gagaacctaa
     8881 aaagacgact gaaagtagca agtcgaacaa gtctaaatct acttcaaata agacagggaa
     8941 atctaaggaa ccatctaaag ctaaatcagc agtagaatat ctatctaaaa ctggtaagaa
     9001 cgtgtatact tcagcagata aagctttagc aaaagcgcca aaaggaagct tagcaggaat
     9061 gacctatgaa gctaagaacg aagctatcaa attaggtaaa ggaactgcta aagttgccaa
     9121 agtaactgct aaaggagcaa ctaaaactgg taaagcagtg aagaaatcta cgatgtcggt
     9181 gaaagattat attgcaaaat atagcgaaca aaaaccaacc gataaaaagt cttaagaaga
     9241 ataaaaagag ggagcattaa tatgctcctt cttttttgtt cttctcttca aggggaatgt
     9301 caagaaaaac ttaattgtct tcaaaaattt cttttttatt tggtagtaaa tctatgaaat
     9361 atttgctttt ttagttatcg gttaataaga gggcttatgc ta
//
LOCUS       DAZUKD010000113         9298 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000113 DAZUKD010000000
VERSION     DAZUKD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.115"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACG8N9_002320"
     CDS             complement(78..2198)
                     /locus_tag="ACG8N9_002320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4897879.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACG8N9_002321"
     CDS             2428..3606
                     /locus_tag="ACG8N9_002321"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4897880.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8N9_002322"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8N9_002322"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4897881.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8N9_002323"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8N9_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4897882.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACG8N9_002324"
     CDS             complement(4959..6212)
                     /locus_tag="ACG8N9_002324"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4897883.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8N9_002325"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8N9_002325"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4897884.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACG8N9_002326"
     CDS             7789..9135
                     /locus_tag="ACG8N9_002326"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4897885.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         2995 a         1680 c         1926 g         2697 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcg
//
LOCUS       DAZUKD010000114         9243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000114 DAZUKD010000000
VERSION     DAZUKD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.116"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8N9_002327"
     CDS             33..656
                     /locus_tag="ACG8N9_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897886.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8N9_002328"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8N9_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4897887.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8N9_002329"
     CDS             2152..3033
                     /locus_tag="ACG8N9_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4897888.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8N9_002330"
     CDS             3036..3806
                     /locus_tag="ACG8N9_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4897889.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8N9_002331"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8N9_002331"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4897890.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8N9_002332"
     CDS             5117..6280
                     /locus_tag="ACG8N9_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4897891.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8N9_002333"
     CDS             6397..6807
                     /locus_tag="ACG8N9_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4897892.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8N9_002334"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8N9_002334"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4897893.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         3208 a         1577 c         2000 g         2458 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccg
//
LOCUS       DAZUKD010000115         9075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000115 DAZUKD010000000
VERSION     DAZUKD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.117"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..1137
                     /gene="yidC"
                     /locus_tag="ACG8N9_002335"
     CDS             187..1137
                     /gene="yidC"
                     /locus_tag="ACG8N9_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4897894.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1316..2290)
                     /gene="lacD"
                     /locus_tag="ACG8N9_002336"
     CDS             complement(1316..2290)
                     /gene="lacD"
                     /locus_tag="ACG8N9_002336"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4897895.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(2287..2745)
                     /locus_tag="ACG8N9_002337"
     CDS             complement(2287..2745)
                     /locus_tag="ACG8N9_002337"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4897896.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKDVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTKIATILTKEIGEDL"
     gene            complement(2742..4208)
                     /locus_tag="ACG8N9_002338"
     CDS             complement(2742..4208)
                     /locus_tag="ACG8N9_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4897897.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDASMGMMVPVLAAYIAEGIAKRPGLIVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDDSKAANRTVIEKGRAGDLS"
     gene            complement(4190..5140)
                     /gene="pfkB"
                     /locus_tag="ACG8N9_002339"
     CDS             complement(4190..5140)
                     /gene="pfkB"
                     /locus_tag="ACG8N9_002339"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4897898.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIREERFERWENIN"
     gene            5283..6032
                     /locus_tag="ACG8N9_002340"
     CDS             5283..6032
                     /locus_tag="ACG8N9_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4897899.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINVLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISQKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            6298..7776
                     /locus_tag="ACG8N9_002341"
     CDS             6298..7776
                     /locus_tag="ACG8N9_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS4897900.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(7844..8935)
                     /locus_tag="ACG8N9_002342"
     CDS             complement(7844..8935)
                     /locus_tag="ACG8N9_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4897901.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         2897 a         1838 c         1521 g         2819 t
ORIGIN      
        1 aagaaacact tggaatcact cttttctttc taagtatttc tttcttgatt ttttgtcttt
       61 tttcaaaatt tttcgctacg aaaaagaaag aatatttata tgaaatttct atgaagttga
      121 ttgaattcca tgcgcttttt tagtatagtt aagtttgtgt aaaaattata gaagaggaag
      181 tattctatga ataaatggaa aaactggtta ttgggatccg gacttgtcag tatcctgctc
      241 tttttatctg gctgcgtgcg gatggattca aacggaaacc ctgacacttc aggaatcgtt
      301 tatcgagtac tcgtccatcc tatgggccaa gcgattacat atctagttga gaatttcaat
      361 tggtcttacg gctgggcagt tattgcaatg actgtgatcg ttcgtatcat catcttgcca
      421 ctaggtatca gtcaatcgaa aaaaacaatg atccaatctg aaaaaatgca agcgttaaaa
      481 ccacaagttg aagcagcaca gcaaaagcta aaagctgcaa caacacgtga agaacaaatg
      541 gcagcacaag cagaaatgca gcaagtatat cgcgaaaacg gattgagtat gaccggcgga
      601 atcggctgtc tgcctctatt gatccaaatg ccgatcttct ctgccttgta ttttacggct
      661 cgctacacag aaggtatccg tgaatcatct ttttacggca tcgatctagg ctcaccaagt
      721 atggtgcttg ttgccattgc aggggtcgct tatctgcttc aaggatatat ttcaacaatc
      781 ggtattcctg aagaacagaa aaagacgatg cgtacgatgc tgatcgtctc tcctgctatg
      841 atcgtcttca tgtcgatcag tgcgccagcc ggtgttacac tttactgggt agtcggtggt
      901 gtgttcagct gtctgcaaac attcatcaca aacgttatga tgaaaccacg tttgaaagca
      961 caagtggcag aagagttgaa acaaaacccg ccaaaacaag tcgtcactcc aagaaaagac
     1021 gtgacagaat cagcaaagcc agctaacaac aaacaagcaa caaaacaaat caaacaacca
     1081 aaaaacagta caaacaacaa aggccgcaac gcaggaaagc agcagaagcg gaagtagata
     1141 gtaaataaga gtgtaataaa aattcattaa cagttttagc aattaaaaat atcgaccaag
     1201 atttttgaaa aagaaatctg gtcgatattt ttatttaagt gaatgtttaa tttgaatcat
     1261 cgattgatcg ctatctaatt gcagcagcat ctttttatga agaaacaact ttttcttatc
     1321 tatcaacgta gacaggtgtt gctgtttctt ctaatagtgt acttaattcc ttaatatttt
     1381 gttttccttc ttctaacatc caactactcg ctttttcttc gccttcggta acaaaaggcg
     1441 ccactccctt cgcccatgtt gctcgcccac ataagacacc attgaatgtc gaacctgatt
     1501 gttttgcaaa acgtaatgta tcttgaaata gcgtagcatt gactcccgca cttaaaaaaa
     1561 taaacggttg ttcagtcact ttcgattgtt catagaaata cgcggatgcc tctgcttgac
     1621 tataacaagt ctcttgccca tatccttcca caaatctcat attcactggc acttctactt
     1681 tcaatacatc cacttgataa cgttcttttg aaaattctcg catggcttca ataactttat
     1741 gtggttttac ttttgcatag gcaagagaag acggatcgcc aatagttgca tcataagaga
     1801 ggatctctaa aaagaaaggt atgccttctg cttcgcattc tgaaccaatc cgttctataa
     1861 ctatttgttt ttgcacatta atctctgctg gttcatcaac gtcatagtac aataaaaatt
     1921 tacaagcatc cgcaccagct tcttttaacc ttttcaccga ccaaatattc aataaatcag
     1981 gtaatcttcc cggaattttc gtatcatagc cggttttttc ataagctagc agcaagccac
     2041 attctttctc tcgaagcgct gctgcaggca ggccgtattc tggatcaagt aaaatagctg
     2101 aggcataggg agtcaggtat tgagaaacca tctgtttaaa ttggatgatg tcttctgatg
     2161 tagcttcttt atattggttg atcattttct tcaaagcacc acgctggtcg attgctaacg
     2221 cgctaataat tccagcttca tccgtcaatc gcttcaaacg ttcctgcttt tctatactca
     2281 tcttcttcat agatcttcac ctatttcttt agttaatatt gtggctattt ttgtttttga
     2341 ttttgctttt ctcaactgtt ctgtaacctc ttttcgcatc aatgatcgtg caacgagaga
     2401 caataatttc aaatgagtcg ttcctgcttc tgaatctgga ataaataacg caaaaataaa
     2461 atcaactgac tgtctatcta aactgttcca ttcaattcct ttgttcagtg tgacgataat
     2521 catactaggt tcgttgattg tttgagactt cgcatgagga atagcaaaac ctgacattat
     2581 tcctgtagta ccttcttttt ctctttctag aagtttttga tatacatctt tttcgtcatg
     2641 agcaattcct aagcagaatg tttgctttgc taaaaactta aatgcttctt cttgactatc
     2701 caatggctgt tgtaaaaaaa tgttctcttc atgaatgatt gtcatgataa gtctcctgct
     2761 cttccttttt cgataactgt acgatttgct gctttactat catccatctt cttgtcttta
     2821 tcagttactc ttttcttttg gattaatcct agtatcactc caccaacgat cgaaccaata
     2881 agaatcgctg taacccattg gatttttcct gttactacag gtaatactaa aaaaccgcca
     2941 tgtggtgctg gaacttggac gccaaacaaa taagtcaaga cagcagcaat tgatgagcct
     3001 aacatcatcg tgggtaacac tcggatcggg tctttcgcag caaacggaat agctccttcc
     3061 gtaatatgag ttgatcctaa aataaaatta accaaacctg cattacgatc attggtatca
     3121 aaatatttac caaacaataa agtagcaaaa ccagtaatca acggtggcgc aatacaagct
     3181 gctgacacac cagccataaa agtcgtgttt ccttgtgcca aaagagctgt tcctgttaca
     3241 tatgccgcct tattaaccgg tcctcccata tcaaacgcac acatgcagcc aacaatcacg
     3301 ccaagaacaa gtggattcga atgttcaaat ccagctaaaa agctcatcat tccttcattt
     3361 atagccttca ttggacctgc cagcaaagac atcacgatgc caataaatgc tacgccaata
     3421 actggatata agaagattgc cttcaagcca tctagttgtt taggtaaaat tgtcagtagc
     3481 ttttgcaaaa ataagataaa ataacctgct aagaacccag ctaaaatacc tcctaaaaat
     3541 ccagtaccgc cattcatggc tacgattcca ccaacaaatc caacgattaa ccctggacgt
     3601 ttcgcgatcc cttctgcaat ataagctgct aaaactggaa ccatcattcc cattgatgca
     3661 tcgccaactg cttttaccat tgccgctgta gcattgtatt gtgcatgatc tggatcaaaa
     3721 gaatagattc cccaaacggc aaatgaaata gcaatcaata ctccgccagc taccacgaaa
     3781 ggcaacatat gagagacacc gttcatcaaa tttttataga tgctacgacc aaaattagca
     3841 ccactcatcg cttctggttc taaagtatca gcagcttcat tatctgctaa gacagtacct
     3901 tttcctgcca atgcttcgtc aatcagccgt tgtgcatcct tgattcctgt actgacagaa
     3961 acatcaatga ctcgttttcc cgcaaatctt tctgaatgga catctttgtc tgcggcaata
     4021 atgacagcat ctgcctgttt gatttcttca gccgttaatt cattttcgat cccaatttgt
     4081 ccatgggtct ctactttgat cgtgatgcct tttttcctag ctgcttgttc taatgcctct
     4141 tgcgccatgt aggtatgcgc aattcctgtc ggacaaccgg ttgctgcaat tagttgatat
     4201 tttcccatct ttcgaacctc tcttccctga ttttgatttg tttacttaat tcctccacat
     4261 ctaaaaaatc tgttaatcct ttgcgaaaag cggttgagct gcctgctgct atgcttttac
     4321 gtaacgtttc atcaagcggt cgtcgattca tgtatcctgc taaaaaagcg cctaacatgg
     4381 tatcacctgc acatgctgta ttgacaactt ttccttttgg cgagtttcct cgaaacaccc
     4441 tgtctttcgt aaaaagaatt gctcctttct caccgaggga taacaaaaca ttttctgcac
     4501 ctaactctac tagtcgttgt ccataaacaa agtaatcttc atctgtaacc atttttcgtc
     4561 cgaaccaaga agaaagttct tcctcgttcg gtttcagcaa aaatggtcga tagggtaaac
     4621 aatccatggc ttcttgatca ctactatcaa taatcaaatg aatccccttt tcctgacaaa
     4681 tccgactgat ttctattaaa acctttggag acagccctct tggtaaactc ccagaaacac
     4741 aaagataatc ttctgcttgt aatgctctta tttgatttaa aaaattatgg acagctgtct
     4801 gcggtatttc aggtccttga ttcactaatt tgtattcttc tcccgtttga ttgacttgtg
     4861 taaatacatt gatacgcgtc ataccaggca cttcaataaa ctctgtttgg atttgtttct
     4921 gctgtaaata gtcttctata tactttcctg tgaacccagc agaaaaacct aatgccgtac
     4981 ttgggaaatc tagcatcttt aatactagtg acacattcac tccttttccg tttgcctgaa
     5041 tgtcatcctc ttttgtgcgg tttaccataa aaggatgcat ctcctctgtt tcaataaata
     5101 gatcaattgc caaattcatc gtgcatgtgt agatagacat aaccaatgac ctccttgact
     5161 ctattatgaa atacgcgaaa gagaaaatgt tttcttaatc gttccaacac aaagaaaacc
     5221 tttacattta taaactcgtc gtgctatttt ttaactaact tttggtgaaa gaaggaaaga
     5281 atatggatca caaattaagt gaaacagaac agtacctctg gaactttatc gaacaccata
     5341 tcctagagat ccctaactac tccatcgtga aactaagtga acaagcaaat gtctcaacag
     5401 ccaccattgt gcggacaatg aaaaagaaag gctatgaagg gtttacttcg tttaagcatc
     5461 atctaaaaga aggagaaaac aagaacatca atttttcttt cttagataaa gtagacaaag
     5521 gtatccgccg agccattcta aaaaatgaac aagaagtggt acgtacgatc aatgtgttgg
     5581 aaatcggaaa tattgaggat gcgatacaaa gaatcaaatt tgctgaccgg gtttttgtct
     5641 ttgcccgagg attttcagaa atgattggcc aagaaatgct ggtaaaactt caattaacgg
     5701 gaaaaaattg tcaaatgcat actgatccag aaattatcaa aagcatcagt cagaaactga
     5761 ctaagaaaga cagcgttatt tttgtttctt tgaacggcga gacaaaagaa ctcgtcaccg
     5821 ccgcaaagaa ttgctatgat gcagagattg gttcgattct agttacagcc aatcattcct
     5881 ctacactgaa agactattgc gagatcaatc tcgtaggttt caaatcagaa ggttcttatt
     5941 tccctgatta tgaagtacgg tcacgcttgc ctctccagat tatcgcgaga attctgcttg
     6001 acgcctatgt attgcgtatg ggggagaatt gagaagcagt ttattatggt tggttacagt
     6061 ttaaaacatc acatttttta ttctttatat tgtatagact cacgtaaact ttttatgtat
     6121 aaagttattc acaatctata aagttacttt aagtaacaaa aaataattga aaatacttaa
     6181 aatcaaaaaa tatcttttta atcaataaaa aaattgacag ttttattaat aataagctat
     6241 tctgttatag aagaaaaaat tgtttgttat tattagcaac aaaaaaagga gtgcatcatg
     6301 aaaaagttat ctcaaaaacg tttagctcaa atcattattg attctagaaa atctctgaaa
     6361 atgacacaac aacaacttgc agtagctact ggtatcaacc gtgccttaat tagtcgatta
     6421 gaatctctcg attttatccc gtcaatacaa caattagaat cattagcaga agttctaaat
     6481 tttgaaccaa cagatctatt tataaatgaa tcaaataaat caaacagaac aaaacatcag
     6541 tcgaagaata ttgctgtagc cggtactggt tacgtaggtc tttccattgc tactcttctt
     6601 gcccagcaca atcaggtaac agcagtagac attcttcctg aaaaagtaaa acaaattaac
     6661 aataagaaat ctccaataca agacgattac attgaaaaat atctagctga aaaaaatctt
     6721 aatttaattg caacgactga tggtgcagcc gcctatagag aagccgattt tgttgtcatt
     6781 gctgctccaa caaattatga tagtaaacaa aattattttg atacttctgc tgtagagtca
     6841 gtaatcaaat tggtgttaga agtcaatcca aaggcaataa tggtcatcaa gtcgacggta
     6901 cccgtaggct ataccgaaag tattcgtaaa aaatacaata ctaaaaatat tctattcagc
     6961 ccagaatttc tcagagaatc taaggcatta tatgacaatc tatatccttc gcgaattatt
     7021 gtcggcacgg accaaactga caaaacttta cgagaagcct ctcacacttt tgcagattta
     7081 ctccaagaag gagcaatcaa aaaagatatc gaaactcttt tcatgggatt cacagaagct
     7141 gaagcagtta aattattcgc taatacttac cttgcacttc gagtatctta cttcaatgag
     7201 ctggatactt atgcagaaat gaaaggacta aatacacaag aaatcattca cggggtttgc
     7261 ttagatccaa gaattggcac acattataat aatccatctt ttggatatgg aggctattgt
     7321 ttaccgaaag atacaaaaca acttctcact aactataacg atgttcctca gaatatgata
     7381 agtgcaattg tcgatagtaa tcgtacaaga aaagacttta tagcagatcg agtgttagaa
     7441 cttgctggca cgtatgaggc taacagttcg tggaatgcag aaaaagaaaa agaggtagtc
     7501 ataggtgtat accgtcttac aatgaagagt aattcagaca acttccgtca gtcatctatt
     7561 caaggagtta tgaagcgaat taaagcaaag ggggcaacta ttgtaattta tgaaccaaca
     7621 cttgaaaata acactacatt ttttggttca aaagtaataa ataatttaga tgattttaaa
     7681 aatatcagtc acagtattat tgctaatcgt tacgatagta atcttgatga tgtaaaagaa
     7741 aaagtatata ccagagattt atttcaacga gattaaaaaa taacacctca gatttgtagt
     7801 gtctgaggtg tttctataaa cttttataag taaaaaaata caattatact tcataaaaag
     7861 acttatacca ctgcgcaaat ttacgaagcc cgtcacgcaa acttgtactt ggtttaaatt
     7921 caaaatctcg ttctaacgca ctagtatctg cataagtaac tggcacatct cctggttgca
     7981 ttggaactag ttcggtatga gattcaaagt cgtaatcttc tggtagcacc cctgctgcaa
     8041 ttagctcttg ttgtagaata tctacaaaat ctagtaggtt ttccggcgta ttgttaccaa
     8101 tattataaat atgatatggt gggattggta gcccatcttc cccatcaatt ttctctggag
     8161 cgcactgcat aaccctcttt actccttcaa caatatcatc tatataggta aagtcacgct
     8221 tgcaattccc ataattgaat atttggatat tatcaccagc acgaagttta tcagtaaaac
     8281 caaaataagc catatctgga cgcccagcag gaccatatac tgtaaagaaa cgtaaccctg
     8341 tagacggaat attgtaaagt ttagaatatg catgtgccat tgattcatta cttttcttag
     8401 ttgctgcata caacgatact tgattgtcta ctttatcctc tgtagaataa ggaattttct
     8461 tattagtacc ataaacggaa gacgatgagg catacactaa atgttccaca ccagattctc
     8521 cattatcata tgaatgacga caagcttcaa gaatattata gaatcctatt atgttagatt
     8581 cgatatacat atctggatta gtaattgaat aacggactcc tgcctgagct gctaaattca
     8641 ccacgatact tggtttataa ttatcaaaga tgaaatcaat tgttgcttta tcagcaatag
     8701 aacctttgat aaaattccaa gtagatcctg gatggttctt tgcctccgtt tctatctctg
     8761 ataatctata gttcttaata gagacatcat agtaatcatt catattgtca atgccaatga
     8821 tttttattga tgactgagta cgtaataact caagaaccaa atttgatcca ataaaaccgg
     8881 ctgcgccagt tattaatatt gttctatttg ataaggacgt gtttaattcc agcatttttt
     8941 agcactcact ttcttttttt cttatgaata catatttgtt atatattaaa aaatcttatt
     9001 tagacttatc tattgtcact tttatcattc taatctttgc acgtagtaga ataattatac
     9061 agataggaaa ttgct
//
LOCUS       DAZUKD010000116         9016 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000116 DAZUKD010000000
VERSION     DAZUKD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.118"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..154
                     /locus_tag="ACG8N9_002343"
     CDS             71..154
                     /locus_tag="ACG8N9_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4897902.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="ACG8N9_002344"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="ACG8N9_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4897903.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1186..1446
                     /locus_tag="ACG8N9_002345"
                     /pseudo
     CDS             1186..1446
                     /locus_tag="ACG8N9_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1609..2274
                     /locus_tag="ACG8N9_002346"
                     /pseudo
     CDS             1609..2274
                     /locus_tag="ACG8N9_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2310..2555
                     /locus_tag="ACG8N9_002347"
     CDS             2310..2555
                     /locus_tag="ACG8N9_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4897904.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2658..3527
                     /locus_tag="ACG8N9_002348"
     CDS             2658..3527
                     /locus_tag="ACG8N9_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4897905.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLLECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3508..4242
                     /locus_tag="ACG8N9_002349"
     CDS             3508..4242
                     /locus_tag="ACG8N9_002349"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4897906.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4275..5183
                     /locus_tag="ACG8N9_002350"
     CDS             4275..5183
                     /locus_tag="ACG8N9_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4897907.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5180..5722
                     /gene="sat4"
                     /locus_tag="ACG8N9_002351"
     CDS             5180..5722
                     /gene="sat4"
                     /locus_tag="ACG8N9_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4897908.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5815..6609
                     /locus_tag="ACG8N9_002352"
                     /note="aph(3')-IIIa"
     CDS             5815..6609
                     /locus_tag="ACG8N9_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4897909.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6885..7016
                     /locus_tag="ACG8N9_002353"
                     /pseudo
     CDS             6885..7016
                     /locus_tag="ACG8N9_002353"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            7092..7837
                     /locus_tag="ACG8N9_002354"
                     /pseudo
     CDS             7092..7837
                     /locus_tag="ACG8N9_002354"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7948..8127
                     /locus_tag="ACG8N9_002355"
                     /pseudo
     CDS             7948..8127
                     /locus_tag="ACG8N9_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
     gene            8230..>9016
                     /locus_tag="ACG8N9_002356"
     CDS             8230..>9016
                     /locus_tag="ACG8N9_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFS4897910.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNI"
BASE COUNT         3162 a         1401 c         1873 g         2580 t
ORIGIN      
        1 ttttgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta cagatgtaat cacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac actcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1081 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1141 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1201 cctaagtgtg gcaacaatat tgtattaaaa aaatcgtttt atggttgttc aaattatcct
     1261 gaatgtaagt ttactttagc tgaacatttt agaaagaaaa aactaaccaa aacgaatgta
     1321 aaagaattac tggagggaaa agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag
     1381 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca
     1441 aaataaacat ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt
     1501 tagatagtag tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca
     1561 ctctgctata atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat
     1621 tataccataa accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat
     1681 tttgctcatg ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat
     1741 tatcaatcta tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt
     1801 gcctatattc aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt
     1861 cggatgcgct acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc
     1921 gttcatccga ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg
     1981 gttcaaagtg gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg
     2041 gatgcgaata aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt
     2101 gatcaaaaag cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta
     2161 atagatgatt taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg
     2221 gaagaaaagg aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc
     2281 tgacccatta ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag
     2341 ctattagaga aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca
     2401 agggatatgt ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt
     2461 taaaccatgt tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc
     2521 agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat
     2581 tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga
     2641 taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct
     2701 ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag
     2761 aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta
     2821 ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg
     2881 gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg
     2941 atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa
     3001 ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag
     3061 gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa
     3121 aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg
     3181 aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata
     3241 ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata
     3301 atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac
     3361 ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc tcacttttgg
     3421 aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa
     3481 ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt
     3541 tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg
     3601 gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc
     3661 ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt
     3721 gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa
     3781 tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta
     3841 agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg
     3901 atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat
     3961 ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat
     4021 tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat
     4081 agaacactga ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt
     4141 gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga
     4201 cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa
     4261 cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag
     4321 aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac
     4381 ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta
     4441 tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg
     4501 atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt
     4561 acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg
     4621 ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac atagttccga
     4681 ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg
     4741 aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg
     4801 ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg
     4861 tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt
     4921 atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa
     4981 acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg
     5041 cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg
     5101 acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata
     5161 tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga
     5221 taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa
     5281 ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga
     5341 ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat
     5401 atatctttac taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa
     5461 ccggtacgct tatatagaag atatcgccgt atgtaaggat ttcagggggc aaggcatagg
     5521 cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc atggactaat
     5581 gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt gtggtttcaa
     5641 aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt
     5701 tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata
     5761 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct
     5821 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
     5881 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
     5941 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
     6001 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
     6061 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
     6121 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
     6181 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
     6241 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac
     6301 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
     6361 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
     6421 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
     6481 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
     6541 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
     6601 ttgttttagt acctagattt agatgtctaa aaagctttaa ctacaagctt tttagacatc
     6661 taatcttttc tgaagtacat ccgcaactgt ccatactctg atgttttata tcttttctaa
     6721 aagttcgcta gatagagttc tatattagag atgtaaagag ttttggtgaa gaggtttgtg
     6781 agtatggctt ttataaccag ggtgcaatga gaagcactaa cagcagctag cttaagaacg
     6841 cataggatat actttttatg gaagtcacag aggaggaata aaatttgaac aggattaaca
     6901 gagtaacctc tattcttatt cagctgcaat caaaaaaaat aattcctgcc aaagaaattg
     6961 cacagcgatt taatataagc ttaaggacag tttacagaga tatccggaca cttgaagaag
     7021 ccggaatacc aattggatct gaggccggaa aaggatattt tcttgtagag ggcttcctac
     7081 ttccgccggt aatgtttaca gcggcggaag tgggtgcatt gattacagca gggaaatttt
     7141 taaattgcca tggagatgaa tcatttataa aggattttga ttcagctatg tataaaatca
     7201 aatctatttt gaagcatggt gaaaaaaact atgcacagga gttggagaac agtattaacg
     7261 tgtacagcac atctggacag aaaaatacat tggcggataa cgttattgca gcaattcaga
     7321 ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc tgcatcagga gggcaagaac
     7381 cggaaagcag aatgatagag cctatatcac tggggtttta tgaacagaac tggtacttaa
     7441 tcggttttgc aggctaagaa atgaatatag gaattttcgt gtggatcgga ttaaaagcat
     7501 ttgcatagaa gaagagctct gtcaaagcca tgatggctct ttggaacaaa tattaaaaca
     7561 aatgctttca tataaaaaac tatataatgt aattcttaga gctgaaaaag gggagacata
     7621 caattctatc aagaacaggt attctctcgg cttccttgaa gaaaccgatt tgggaagtaa
     7681 aatggaaatc gaattccaga cagattcctt tgagatattg agcaagcagc tgattgaata
     7741 tggatcaggc attgaaatag tccaacctga tgaattaaaa tgcataaccc gtaaacacct
     7801 ggcacaaatt acgaatcact gcctaaactt gatttagaga aagcgattgg tctgttatgt
     7861 attttttgta aggtttcata tcccctgctg acatacagct gtcagtgtat tatgatattc
     7921 tactatcaaa aaggaggatt gaaaaatatg aaaccaaaga taattatgca cacacaaatt
     7981 agccttgatg gccgcattaa aggctttgat aatccggaag tttactacca ggttgccggg
     8041 ggaattcatt cggatgcggt gctgtttggg tccaatactg tttttactgc atttgaaaaa
     8101 tatcctgctg aaacagaggc tgattttgga taaagtccgt ataattgtgt aaaagtaaaa
     8161 aggccatata acagtccttt tacggtacaa tgtttttaac gacaaaaaca tacccaggag
     8221 gacttttaca tgacccaagt acattttaca ctgaaaagcg aagagattca aagcattatt
     8281 gaatattctg taaaggatga cgtttctaaa aatattttaa caacggtatt taatcaacta
     8341 atggaaaatc aacgaacaga atatattcaa gcaaaagaat atgaacgaac agaaaaccga
     8401 caaagtcaac gaaatggcta ttatgagcgc agctttacga cacgtgtagg cacgctagaa
     8461 ttaaaagtac ccagaacacg tgatggccat ttttcaccca cagtgtttga acgttatcaa
     8521 cgaaacgaaa aagccctcat ggcttcaatg ttggaaatgt atgtatcagg cgtttcaact
     8581 cgtaaagtat caaaaattgt ggaagaactt tgtggtaaat ccgtctctaa gtccttcgtt
     8641 tctagcttaa cagaacagct agaacctatg gttaacgagt ggcagaatcg tttattatca
     8701 gaaaaaaatt atccttactt aatgaccgat gtactctata taaaagtacg agaagaaaat
     8761 cgagtactct caaaaagctg tcatatagcg attggaataa ccaaagatgg cgaccgtgaa
     8821 attatcggct tcatgattca aagtggcgaa agcgaagaga cctggacaac attttttgaa
     8881 tacctaaaag aacgcggttt acaaggtacg gaactcgtta tttctgatgc gcacaaagga
     8941 ttagtctctg ccattagaaa atccttcacc aacgtaagtt ggcaaagatg ccaagttcac
     9001 ttcctaagaa atatct
//
LOCUS       DAZUKD010000117         8857 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000117 DAZUKD010000000
VERSION     DAZUKD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.119"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(472..696)
                     /locus_tag="ACG8N9_002357"
     CDS             complement(472..696)
                     /locus_tag="ACG8N9_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897911.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            887..3664
                     /locus_tag="ACG8N9_002358"
     CDS             887..3664
                     /locus_tag="ACG8N9_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4897912.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3745..4164
                     /locus_tag="ACG8N9_002359"
     CDS             3745..4164
                     /locus_tag="ACG8N9_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897913.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4176..4646
                     /locus_tag="ACG8N9_002360"
     CDS             4176..4646
                     /locus_tag="ACG8N9_002360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4897914.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4663..5442
                     /locus_tag="ACG8N9_002361"
     CDS             4663..5442
                     /locus_tag="ACG8N9_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4897915.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5429..6250
                     /locus_tag="ACG8N9_002362"
     CDS             5429..6250
                     /locus_tag="ACG8N9_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4897916.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6267..7316
                     /locus_tag="ACG8N9_002363"
     CDS             6267..7316
                     /locus_tag="ACG8N9_002363"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4897917.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7329..8333
                     /locus_tag="ACG8N9_002364"
     CDS             7329..8333
                     /locus_tag="ACG8N9_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4897918.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2958 a         1466 c         1833 g         2600 t
ORIGIN      
        1 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
       61 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
      121 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
      181 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
      241 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
      301 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
      361 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
      421 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
      481 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
      541 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
      601 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
      661 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
      721 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
      781 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
      841 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
      901 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
      961 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
     1021 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
     1081 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
     1141 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
     1201 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
     1261 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
     1321 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
     1381 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
     1441 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
     1501 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
     1561 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
     1621 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
     1681 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
     1741 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
     1801 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
     1861 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
     1921 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
     1981 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
     2041 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
     2101 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
     2161 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
     2221 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
     2281 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
     2341 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
     2401 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
     2461 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
     2521 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
     2581 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
     2641 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
     2701 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
     2761 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
     2821 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
     2881 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
     2941 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
     3001 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
     3061 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
     3121 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
     3181 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
     3241 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
     3301 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
     3361 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
     3421 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
     3481 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
     3541 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
     3601 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
     3661 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
     3721 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
     3781 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
     3841 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
     3901 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
     3961 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
     4021 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
     4081 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
     4141 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
     4201 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
     4261 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
     4321 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
     4381 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
     4441 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
     4501 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
     4561 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
     4621 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
     4681 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
     4741 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
     4801 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
     4861 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
     4921 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
     4981 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
     5041 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
     5101 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
     5161 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
     5221 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
     5281 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
     5341 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
     5401 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
     5461 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
     5521 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
     5581 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
     5641 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
     5701 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
     5761 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
     5821 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
     5881 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
     5941 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
     6001 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
     6061 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
     6121 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
     6181 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
     6241 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
     6301 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
     6361 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
     6421 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
     6481 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
     6541 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
     6601 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
     6661 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
     6721 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
     6781 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
     6841 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
     6901 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
     6961 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
     7021 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
     7081 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
     7141 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
     7201 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
     7261 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
     7321 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
     7381 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
     7441 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
     7501 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
     7561 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
     7621 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
     7681 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
     7741 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
     7801 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
     7861 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
     7921 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
     7981 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
     8041 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
     8101 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
     8161 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
     8221 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
     8281 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
     8341 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
     8401 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
     8461 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
     8521 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
     8581 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
     8641 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
     8701 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
     8761 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
     8821 agactattgt aacatatcat tgattatttc aagtaaa
//
LOCUS       DAZUKD010000118         8648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000118 DAZUKD010000000
VERSION     DAZUKD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.120"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..751
                     /locus_tag="ACG8N9_002365"
     CDS             278..751
                     /locus_tag="ACG8N9_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4897919.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            748..2880
                     /gene="feoB"
                     /locus_tag="ACG8N9_002366"
     CDS             748..2880
                     /gene="feoB"
                     /locus_tag="ACG8N9_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4897920.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2882..3016
                     /locus_tag="ACG8N9_002367"
     CDS             2882..3016
                     /locus_tag="ACG8N9_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4897921.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3110..3754
                     /locus_tag="ACG8N9_002368"
     CDS             3110..3754
                     /locus_tag="ACG8N9_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4897922.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQAKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3941..5134
                     /locus_tag="ACG8N9_002369"
     CDS             3941..5134
                     /locus_tag="ACG8N9_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4897923.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5127..6854
                     /locus_tag="ACG8N9_002370"
     CDS             5127..6854
                     /locus_tag="ACG8N9_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4897924.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7248..7445
                     /locus_tag="ACG8N9_002371"
     CDS             7248..7445
                     /locus_tag="ACG8N9_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4897925.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7447..7758
                     /locus_tag="ACG8N9_002372"
     CDS             7447..7758
                     /locus_tag="ACG8N9_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4897926.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            7862..8008
                     /locus_tag="ACG8N9_002373"
     CDS             7862..8008
                     /locus_tag="ACG8N9_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS4897927.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            8084..>8648
                     /locus_tag="ACG8N9_002374"
     CDS             8084..>8648
                     /locus_tag="ACG8N9_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4897928.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRIT"
BASE COUNT         2854 a         1397 c         1836 g         2561 t
ORIGIN      
        1 gtttttgaaa aaagagctga aaacaagtat cgtgtgggaa aatagcaaat ttaatagaga
       61 aaaaattgtg gaaaactatg aagaaatatt tttttctcat ttttatctat caatactaaa
      121 gattcgctca taatatcaca agtataattg acattctcaa ttaccgagag tattctatgt
      181 gagaatgatt ctcatttatt tgcgtaacac gtaagaaaat tagaacatat aatgaatgat
      241 tttaatacat gatttatttc acaagagagg aatcaatatg cagaccttag cgcaggcaaa
      301 gcagaatttc gtttatcaag tcgaacgaat cgaaacagaa gaaaaaacaa aacgccattt
      361 gcaaaatatc gggatcgttg ccggaagtaa aatcgtcgtc gtcaatttat caggcaacaa
      421 tgccatttta ctggtaaaaa gtcatcgagt ggcagtaagt cgggaaatac taggtcaaat
      481 ctttgtcaat aaaaagactg cttcctctga aagctggact tctcttgatc aattaaaagc
      541 aggagagact gggatcgtcg tagcaatcca cggacaaggc gcagtgaagc gacggttaat
      601 ggatatggga ttgacgcgaa atgtaaaaat agttatccga aaactagccc ctctgggaga
      661 tccaatcgaa ctgacagtgc gagggtatga actgacgtta cgaaagtcag aggccgaact
      721 aatcttgata caaagggagg aaaaagaatg aatatcgcgt tagtcggaaa tccaaacagc
      781 gggaaaacta gcttgttcaa tctcttgact ggttcgaatc aatatgtagg gaattggccg
      841 ggcgtaacag tagaaaaaaa agaaggatcg ttgaaaaaaa atccgaacat tcatattcag
      901 gacctacccg gcatctattc tttgtctcct tatacaccag aagaagtgat tgcaagagat
      961 tatttggttc aggaacagcc tgaactgatc atcaacatta ttgatgctac caatttagaa
     1021 cgaaatcttt atttaactac gcagttattg gatttagaca ttcctatgat cattggtctg
     1081 aatatgatgg atgtcatcaa aaaagaagga aaaaaaatca atctagaaaa gctttcctat
     1141 ggtcttggtg taccagtttt accaatcagc gtattgaaaa agcaaggaat cgaaaaacta
     1201 atcgaaaaga ttcaggagtc tgcaaaaaag cggccttcta ttcctgttcc ctgtacatat
     1261 gatccacgtt tagaagcagc tttacatgaa attgaagatg tactaggaga acaaagcggc
     1321 aagaaacgct ggtatgcaat gaaatatttt gaacgagatc agaaggtaag agaggattac
     1381 gagatagcag tttctcagca aaaagagatc gagcaactga tccagttaac agagaaacta
     1441 ttggacgacg atagtgagac gattctggtc aatgaacgtt atgaatttat tacccaacta
     1501 tgtacactct gtgttgtgtc aaatgattct ttccaattat cgatgagtga caaaatcgat
     1561 cagattgcaa cgaatcgttg gttagccttg ccgatcttcg catttgtgat gtggctgatc
     1621 tattatctgg ctattcaaac agtaggaaca atggggacag attggatcaa tgacacattg
     1681 tttggtacat ggttaccaga acatgttggc agattgttag ctgaatggca agtagcggga
     1741 tggatgcagg agctgatctt gaacggcatc atcgcaggcg tgggagcagt gttaggattt
     1801 ctaccgcaat tgattatatt gtttttatgc ttgagctttc tggaagactg cggatatatg
     1861 gcgaggatcg cttttgtcat ggatcgactt ttcaggaaat tcggtctttc aggaaaatcc
     1921 ttcattccta tgctgattgc tacaggatgt ggagtgccag gcgtcatggc cagtcgcacg
     1981 attgaaaacg agcaagatcg tcgtttgacg atcatggtca caaccttcat gccttgttct
     2041 gcaaagttgc cgattattgc cttagtagca ggggcctttt ttcctcatca atcttgggta
     2101 gctccttctg cctattttct tggtatgact gcaattattt tctctggaat cagcttgaaa
     2161 aaaacacgtt tgtttgctgg agatacagct ccttttatca tggaattgcc cgcatatcat
     2221 tttcctcaat ggtcagcggt cctaagacaa acctatgacc gcagcaagtc ttttgttaaa
     2281 aaagcaggaa cgattatctt tgtttctagt atcgtgatct ggttcagctc ttcttataat
     2341 ttttatgctc aacctgtttc tgaagaccaa agtatcttag cctttttcgg gcgaatcctg
     2401 gcagttgttt ttcagccgct tggttgggga aactggcaag gaacagtcgc agcgattaca
     2461 ggattggtcg ctaaagaaaa tattattggc acatttggta ttttgtttgg tcatgcggaa
     2521 gtatccgaaa atggcagaga aatctggcaa gtgctacaac acacatatac tcccgcagca
     2581 gcttattcat tgttagcctt caatttactt tgtgcgcctt gttttgcggc aattggggct
     2641 atccatcggg aaatgaatgc caagaaatgg acttggatcg ccataggcta tcaatgcggt
     2701 ttagcttatt tagttagttt tgtgatttat cagttgggac acttattgga gggtggacag
     2761 gtagcgttag ggacgtatct tgctatttta ctgatgatag gactcgttta tgttttgttc
     2821 cgtcagccaa aatccaagaa ccagttttat actatattgt cattagaagg agaggaatag
     2881 gatggcgaca ttcttattaa gtgtgttaat ctttggaagt gcaggcgcaa ttgtctatac
     2941 tcgtttaaaa tccggtaaga attgtgatga ctgtcaaaca gcttgcccag taaaaaagga
     3001 acagatcaag cgatgatcag tcgttgcaag aggctattct cccctttttt gcttattttg
     3061 ctagagcata gtaagatgag ggaaagtaaa aaaagaaaga ggaaagaaaa tgtctggaaa
     3121 gaaaaaaatc gtccgcccgc gctaccagca gattgctgtt gatttagccg aacgtatcgt
     3181 tgaaaaccga tataaagtgg gtgagaagat ccatgcccgc tcaacacttg ccagtacgta
     3241 caatgcttct cctgagacta cccgcaaggc tatcaatgtg ctggtagatc ttggaatcat
     3301 ggaagtacgg catggaagtg gggcatttgt tgcttcgaag gaaaaagcaa aagatttcct
     3361 attgcaatat caagatgtac aatccatcca agatacaaaa gcagaaatca tggcaagtgt
     3421 ggagaagcaa caagatgagc tgaatcattt ttctcaatta ttggatcagc tggtcaatca
     3481 agcgaagcga atacataaga tgaatccttt acttccgttt gaattatctt tatcagaaaa
     3541 agcaatgcat ctggaccgtt caattagcga gttgaatatc tggcaggcga ctggcgccac
     3601 agttatcgca atttctcata atgaagaact gcttgtttca cctggcccct atgctaaatt
     3661 tacggcaggg gacatcgttc attttattgg aaatgaatat acactacagc gtatgacaaa
     3721 ctttttttac ccggagcatc agcttgcaga atgattgtgt gaaatattaa aaaaatatta
     3781 acttggctaa accttttatt tacaaagatt tagccaagtt tttttcagtt tggacaagca
     3841 aatttctttc atttttgaca aagtgactac atgatgttaa catagtgtgg tcactttttt
     3901 cagaaagaaa aatgaattta cataaaaaag gagaatttgt ttggtaaaag tagaagtaaa
     3961 acatttaaca aagatatttg gtaaaaagac acaagccgct ttagatatga tgaatgatca
     4021 ccagccgaaa acggagattt taaagaaaac cggtgcaacg gttggtgtgt atgacgtgaa
     4081 ttttgacgta aaagaaggag aaatcttcgt gatcatgggg ctttccggga gtgggaaatc
     4141 cacattgatc cgtctattga atcgtctgat tgagccaact tcgggcagta tctatattga
     4201 tggagaagat gtatcgaagt taagcaaaga agaattgcga gaagtccgtc gtcataaaat
     4261 caatatggtt ttccaaaact tcggtctgtt tccacatcga acgatcttgg aaaatacaga
     4321 atacgggtta gaagtccgtg gtgtacctaa agaagaacga caagaaaaag cagaaaaagc
     4381 attagaaaat tctagtttgc tttcatttaa agaccaatat cctagtcagt tatctggtgg
     4441 gatgcaacaa cgggttggcc tagccagagc tttagcaaat gatcctgaga tcttgctgat
     4501 ggacgaagcg ttctcggctc ttgatccgct gatccggcga gaaatgcaag acgaattact
     4561 ggacctgcaa gccaatgttc aaaaaacgat tattttcatt acccatgatt tgaatgaagc
     4621 tttacggatt ggggacagaa tcgccttgat gaaagacggc gaaatcatgc agatcggaac
     4681 aggtgaagaa atattgacca atccagcaaa tgattatgta cgtgaatttg tggaagaagt
     4741 cgatcgttcc aaagtgctta cggctcaaaa catcatggta cctgcattga cgacgaatat
     4801 cgaatctgat ggaccaaacg ttgcattgac aaggatgcgt aatgaagaag tcagcatgct
     4861 gatggctgtt gacagaaaga gacacctgaa agggatcatc actgcagatc aagcgttaga
     4921 agctaggaag caaaaacgtc cattgatcga cttcctagat gaaaatgtga cggtgattgg
     4981 gaaagacatg gtggtaagtg atattttcaa tattatttat gactcaccga ctccacttgc
     5041 agttgtagag gatggaaaat taaaaggtgt cgtcatccga ggtagtgtga ttgaagcatt
     5101 agcagaaaca agcgaggtga gtgaacatga ataatctatt gaactatcaa ttaccagtgg
     5161 caaactgggt agaaaatatc acagaatggt ttacgacaac tttttctggc ctattttcat
     5221 ttttacaaac gattggacag gcagtgatgt cggggattac aaatttactg ttagtgattc
     5281 cagctccttt gtttatcttg ttattgacgg tagcagcttt ctttatttct aagaaacgtc
     5341 caggacttac gttgttcaca ttaatcggtc tatggtttat ttataaccaa ggtctatgga
     5401 atgatttgat gaatactgtc acacttgttc ttttatctag tgtgatctcg atcatcattg
     5461 gggtaccatt agggatcttg atggcaaaaa gcagtaaagc ccaaagtatt atcaaaccga
     5521 tccttgactt tatgcagaca atgccaggat tcgtttattt gattccagca gttgcatttt
     5581 tcggtatcgg gatggttcca ggggtatttg cctcagtcat ctttgctctg ccaccaaccg
     5641 ttcgttttac gaatttaggg attcgacaag ttcctaaaga attagtagaa gcttctgatt
     5701 cattcgggag cacaagtcgt caaaaactat tcaaactaga attgccttta gctaaaagta
     5761 cgatcatggc agggatcaat caaacaacaa tgctgtcact ttcaatggtc gttattgcct
     5821 ctatgatcgg tgcacccggc ttaggacgcg gcgtattatc agcattgcaa agagcacaag
     5881 tcgggaatgg atttgtcaac ggggttgcat tagttatctt agcaatcatc attgaccgtt
     5941 ttacacagca tttgaaccag ccaaacaata aaaaagctgc aggagcaaca acacagaaaa
     6001 gtaaaaaacg tcaaggactg atcattggtg ctgtagtagt agtgatctta ggtgcgattg
     6061 gtatagggag tttttcatct gccaaagaaa caaaacgtat caacttatct tatgttgaat
     6121 gggatacaga agttgcttca acaaatgtag tcggtgaagt attgaaacaa atgggctatg
     6181 atgtaacgat gacgccatta gataattcaa tcatgtggaa atctgtttca aatggcgagt
     6241 cagatgccat ggtttcagct tggctaccta aaactcatgg ttcacaatat gcacaatata
     6301 aagatcaagt cgaagaccta ggtgccaatt tgacaggagc aaaagttggt ttagcagtgc
     6361 cagcctacat ggatgtaaat tcgatcgatg agttaaccga tcaggcaggt aagaaaatca
     6421 taggaatcga gccaggagct ggtgtagtta cggctgctga aaacacgatc caaaaatatg
     6481 acaacttgaa agactggaaa gtagagacat cttcttcagg agcaatgacc gttgcactag
     6541 gtcaagcgat caaaaaacac gaaccaattg tagttacagg atggacacct cattggatgt
     6601 ttgccaaata tgatctgaaa tacttagaag atcctgaaaa cggaatggga agcgaagagc
     6661 agatccatac gatggttcga aaaggattga aagaagacca gccagaagct tacaaagtat
     6721 tagacaattt ccattggtca gaaaaagata tggaaaaagt tatgttggaa atcaacaatg
     6781 gaaaagatcc acagcaagca gcaaaagact ggatcaaaga gaaccaagaa ttagtcgaaa
     6841 gctggaaaaa ataaaaagac agacacgaaa taaatggagc ttccaaaatt ggatcaaata
     6901 tccaaatttg gaagctccat gttcgtctct atcttttttg tctataaaaa aagagaatgc
     6961 gtagctcatc ttcgaaacgt ccaaagacgc cgacaaacta ttatgaattt atgggtgcga
     7021 gtcaaagaga tcgatcgtag gtatgaaaga ctaatgtgat taaagtttat tatgtacgaa
     7081 gtgcattctc aaaaaatatt aaagaatata catagtttat atacttaaaa gtgattaaat
     7141 tcttattcag gaattatttt gctacgtttc aagaaggaat tgttttttga aatttaatga
     7201 atgttagaat aatagtctaa aaacattaat ttaggggtga ttagattatg aaacatttaa
     7261 aaattttgtc tattaaagag acacaactta tctatggggg taccactcat agtggaaaat
     7321 attatggaaa tggagtgtat tgcactaaaa ataaatgtac ggtcgattgg gccaaggcaa
     7381 ctacttgtat tgcaggaatg tctataggtg gttttttagg tggagcaatt ccagggaagt
     7441 gctaaaatga aaaaaaatgc taagcaaatt gttcatgaat tatataatga tatatctata
     7501 agtaaagatc ctaaatattc tgatattctt gaggttttac aaaaggtata tttaaaatta
     7561 gaaaaacaaa aatatgaatt agatcccggt cctttaataa atagattggt gaattatcta
     7621 tattttactg cttatactaa taaaataaga ttcactgaat atcaagagga attaataaga
     7681 aatttgagtg aaattggaag aactgctgga ataaatggtt tatatcgagc agattatgga
     7741 gataaatctc aattttaatg atacttataa tattaaatat gtctcaaaat tttttgatta
     7801 gaaaaatagg tatattgaat cagtgacata aaagtattta tagtttgtag ggggaatcgt
     7861 aatggaagaa aaaaatagac taaatgctaa acaatgttca gatcaagagc ttaaaaaaat
     7921 taaaggaggt gctggaacaa aacctcaagg aaaaccggca tcaaatttag tggaatgtgt
     7981 tttttcatta tttaaaaagt gtaactaaaa ttttttttat gatatcctaa aatcaggagg
     8041 ggatctcttg attactatcg atacaaaagc ttatctatta agaatgttcg tgggaaactt
     8101 tttctttttt ctcttatttt atatgactaa tactgatctt aacaaaagaa aatatctttt
     8161 tgtatgttta ttgataccac tcactttttt tgttgaatta tttactgatg tttccgcagc
     8221 tatacctata atcggaagtt attttatatt aaaggaaaag aagaaaacta atatagtgct
     8281 tttaaataaa ttactattat gtatgcttat tgatgatttc agatctatta ttacttccat
     8341 ggttatgacg tatatgtttt catttgaagg gataaaaggg tatggttatg tagtattcca
     8401 gataatgctt gattctttgt ttttgctgat gtttatttgg ttatatagaa aattagctat
     8461 aaataggatt gttgaacagt ccagttctaa acttactgct atattcatga tttatttact
     8521 tacaattggt ttatttgttt cttatgttgc tcatcaatat caagtatttg atcattttgt
     8581 gtttggtgtc ttaatatttt taatcattca aatgtttgtt gttctatttt tatttattag
     8641 aataacta
//
LOCUS       DAZUKD010000119         8535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000119 DAZUKD010000000
VERSION     DAZUKD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.121"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..356)
                     /locus_tag="ACG8N9_002375"
     CDS             complement(<1..356)
                     /locus_tag="ACG8N9_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897929.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFY"
     gene            complement(363..911)
                     /locus_tag="ACG8N9_002376"
     CDS             complement(363..911)
                     /locus_tag="ACG8N9_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4897930.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(956..1288)
                     /locus_tag="ACG8N9_002377"
     CDS             complement(956..1288)
                     /locus_tag="ACG8N9_002377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897931.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1302..1559)
                     /locus_tag="ACG8N9_002378"
     CDS             complement(1302..1559)
                     /locus_tag="ACG8N9_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897932.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1754..2308)
                     /locus_tag="ACG8N9_002379"
     CDS             complement(1754..2308)
                     /locus_tag="ACG8N9_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS4897933.1"
                     /translation="MTRNVKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIE
                     AVKETSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQA
                     VRDYMTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVL
                     LQLKVDQEAQKIDAIKIYYVRSAY"
     gene            complement(2317..3408)
                     /locus_tag="ACG8N9_002380"
     CDS             complement(2317..3408)
                     /locus_tag="ACG8N9_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4897934.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3416..5626)
                     /locus_tag="ACG8N9_002381"
     CDS             complement(3416..5626)
                     /locus_tag="ACG8N9_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4897935.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5642..8170)
                     /locus_tag="ACG8N9_002382"
     CDS             complement(5642..8170)
                     /locus_tag="ACG8N9_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4897936.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8222..>8535)
                     /locus_tag="ACG8N9_002383"
     CDS             complement(8222..>8535)
                     /locus_tag="ACG8N9_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4897937.1"
                     /translation="VNAATFLLVVIFEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCV
                     LALAKLKPDGKKVHFYLWDWFCYMIRMMNGDCVYYNWDKVSNNALEELLFVNKYVYLE
                     D"
BASE COUNT         2622 a         1855 c         1216 g         2842 t
ORIGIN      
        1 atatagaaat acattctttt ccagttacat ttaattttta aacaaaattc tccccaaaaa
       61 ggaacttttt ctagtgaaaa cttggtaatc tctttgtaat taattcttgt taaagaataa
      121 ttaaacactt catttttact tgttattttt tcattgacta taactagttc atctggtaaa
      181 aaaattaacc aacaaacatc gacaaacagt aaatgatata atccatttcc acctccaagc
      241 atattcaata ctggtgaaca tgccttagaa acacgaatat agttatggtt taaatagata
      301 ttttctttaa acattgcttg tttaaaagcc atatgctctt gttctaaact tttcataaac
      361 ttttactcta atgcacagct agcagcagct gctgcaattc ctgctacaat atttacctta
      421 gctaactctt tagctgcagc ttttcctaat tgtttttctg caatttttaa taataaatcc
      481 gcagcttttt caaaattaaa agcttttaat gcttcaataa aatctccctg cgtacctata
      541 gcccaagcca ctcctcctcc aggaagagaa ttaatggcta cacatccagc atatcgtttc
      601 caatcaactt tctccattct aggaacaata atatgttcat aaaagactct tgctgctctt
      661 ccttgttcgt tatcttgact gattcgttca cgaatagcac caaaatcctt tatatggtat
      721 acaccatgtt cgtctaaaaa accaactgta tcaaaataaa aagttaattc atctactaat
      781 ttattcttta tttctaaatc aactgtattc actttttctg tggcatacac acttgttcca
      841 gtactaacaa aagtacttaa acctaataag aaaacaccta aaacaccaaa tattttaatt
      901 attcttttca taatttctcc tccataaaaa ataagttact ttttaaatat cttgtttatg
      961 aattcattcc ccaaaggcct tttaagattg atacagcagt ccgttcactt cttttagact
     1021 tgtcatacaa gtcttttaaa tgatcccata tcagactagt ttgtctatct agcttcaatc
     1081 cattaaacgc taatgaacgg ttcatttttt ctattacttt agataaagct acagaaattt
     1141 ttataccttc acaacgcgca tcttggattt caataatagt atcatcaatt agtttttgaa
     1201 tttcttgcct atcaacatat tcaatcttag aaagagaatc ttttaaagct actaatgatt
     1261 caacgatttc ttttttctct tcagacattt tcttcctcct tctagtgacc cgtaaagcca
     1321 tgtagagcag agtccattga accagtaatt acactatcta caacgccatt ccaatttact
     1381 ctacattttc catttttcag atttgtcggt ccatacggat agcctttttg acattcaatg
     1441 ttcggattat accagatagg attataccat tcagccgcag aagcagattc agtcgataca
     1501 aacaatccca caccaacaaa tccaatacac atcacactat agccaattag ttttttcatc
     1561 tcgtatccat ctcctaaaaa atagaatagt tcttatagtt attcataaga catatttttt
     1621 ccgtttttgt tttatccttt ctgtcctata acaacttttt tcaagtaaag aatgacatag
     1681 tttttcttaa aaaaacagtc tgtttttttc tatttcttcg gtgtataata aatatgagtg
     1741 ttcatttttt attttaatac gcactccgta cataataaat cttgatcgca tcaatctttt
     1801 gagcttcttg atctactttc aattgaagta atacaaattg agtaacctta ttcattgttt
     1861 cttctccaaa taacaaatat tgatccggat tctctatatc ttgtgtcacc tggtaaagtt
     1921 tcccagttgt tttcatttgt acatgcgggt ctacatcaat actattgtct ttaatacaac
     1981 gatctgtcat atagtccctc acagcttgat tgcgtttata aacatctgtg aaattactcc
     2041 attttctagc aaaatctttt aagaaatttt ttgtcatttc atctatttgc atttgttgcg
     2101 ctgtctcgtc ttctaatgaa ccaatttgac tgttactttc agtactttga agagttccac
     2161 ttgtttcttt tactgcttcg attgtttctt ctgtcgttct caattgtgtt ttttgttttt
     2221 cagaagaaaa ccaatgacaa ccagtaagac ctatcaaaca taaccaacta aatactaaaa
     2281 ttatcccttt tttcacgttt ctagtcactg cctttcttat ttcaccttac gaaccagact
     2341 acgaattttt gtttgattgt acgtgcgttg ataccgagcc ataatccgtc cttgctcggc
     2401 attctgttcc acactagaga aatgttgtcc gccatatgaa acatctgtta taattccggt
     2461 gtgaccatag attccaggag acaatgcacc acctgcttgc caattcacca catctcctgc
     2521 ttgtaaatca cttggttttg ggttcatgat tacttgccaa ccataggttg tccagttata
     2581 atcttcccct attaacatcg caaacatgtg tccagaaccc attaactgtg ggccacctaa
     2641 ttgagataca taccatgccg taccaccata acattcccca ttatttacgg atcgtccaat
     2701 gatccgatcc aaaaccttcg cattgccttt actttttcct gcaataagcg aagttttttt
     2761 tgttggtagg ctattccaat gcttgatcgc aaaagtatca tattccgtta agttatagga
     2821 cgttatgata ctcattgctc ctcgataata cggactccct tcggtaatac ctgctgccgc
     2881 atagcctcca cgacctagaa tcaataaaca acttccagga tctttattgc ccacacattg
     2941 attaaagttg ctttggctcg ccaccataaa gccataatag tcataacaag cttttaagtt
     3001 cggaaacttc atatagaaac caccttcaga accaccaatt ccacgtgcag aaccttgtgg
     3061 gtagggacaa ccaggaaacc aggtaatccc aaaaaagttc gtatcgtttc ttccagattg
     3121 tgactgtcca taagctgatt catagcctaa ttgagaaaga atcacactcg gtaataactg
     3181 atactttaca gctgcaccaa ctgctgcttg cgcatgttca tctgtgattg gaccacgtcc
     3241 tttaatctct gaaaaaatcg gtaatccttc ggtatattcc ccattaaaag cgacagctag
     3301 acctgtagct tctcctccat gctcagaaag ttctcctgaa aaaaagaatg gcaatagaaa
     3361 aaagggaaga ataaagaaaa gaagacaacc tagacaccct ttcttcattt cttcctcatc
     3421 ctttcttcga taatggtttt tggactgttc gtccttttgg taaagtaaaa tcagttcctg
     3481 gttttgcgtc caaaatcggc agtgttttag gatccttttc tattttttcc gattgctctg
     3541 ttggcttatc taaagaagaa aatgcaggtt gtaacgattt tggtgcggaa atcgacggtt
     3601 tactttcaga cgaacgttgc cccggttgtt gcacagaacg atttcctgat agagtggcta
     3661 ccgacgaatt aggaacagag tattggttca ataactgctg cattgctgga taactcccat
     3721 ttttcgcctg atttttcact tggcgaatct gttgtgaaag agaaccagca gctgttaatt
     3781 gtggcacggg tacttttcca gaaggcaacg gaatcgattg accaacaaaa gcgatacctt
     3841 tatttaacaa tggttttctt tgattattcc cttgttgctt actcaattgt gccaatgatt
     3901 gtacctgttg ttttggtctt actgaattca tggacgaatt tggaatcaaa ccatttgaaa
     3961 gaccagaatt ccactctgtt ttcgatttgg tagaagacat ttcaggctct accccatgat
     4021 aacgaacatg acgaccacta taaggcaagg cttgatttga acgcccacct agttcaaagg
     4081 gattaaccac ttgcctccct tttaccgatt cttgtggtgt acgtttttgt tgaaactttt
     4141 gaaatccatg tatcgctgca gaagcgagtg gattcgttgc tcgatacaca ttccctgcta
     4201 atcctgcacc tttgattgcc aaattttgtc cagcagttga tagttgacca ggcagttgtt
     4261 tcatttgaga caaagaatga cttgctatgt ttttcgtgtt gtttatagtt tgatctgcag
     4321 ctcctaaccc tagtttcttt aataaatctt tccgttttac aatgagtaaa attgatagga
     4381 gtgctttgac aaacatattt cctaaaaaac caaaaacagt attcattgga attaaatcgt
     4441 ctactatgtt atagatcaaa aatgccacca aaataatcac actgtaaaaa gatttttgaa
     4501 acatcaagcc caaaaaggac tttaaggtat tcatgagcat ataatcaaat actggaacaa
     4561 aagaagcgag taatgaaaaa ggaatcacaa gtaataaaca caacaccaac atttgaaaga
     4621 taaaacgaat catgccaatc actacaagtg gtaatccaat acttagatta agcaatggca
     4681 ccatagaacc aacgactgct ttataaacag ctttagttgg ttgaatatag acacgataag
     4741 ccttcttttt ccctctatct tctcctgcag cagtggtcac ttttgtctca atatcagata
     4801 attcttcact tgacgcttcc ttggctagaa attcttgtgg ttcaatctct gcttgataca
     4861 attcttctaa atttgttgtc ccataattta acaatatata cggatcaaca acagccgctt
     4921 tataataaag ttggcgcact ttttgaatgg cttccttttg tgtcgttggc actaaaacac
     4981 cattgacatc ttttgtcgcc aattcatcta gactatttgt agcagaaaaa ataaagcctt
     5041 ctacctccac ggacaaatca tttaaccctt tggtaaaagc ttctccttta ctaggtacgt
     5101 tactaccata gccaaaccaa accattgaaa aaatgacaac gaagagaaat cgaataaaca
     5161 ccatttttgc gtaatgtttc cctctaaata tacgaagagt aaatagatat aacacataca
     5221 agacgacaat ccccgcacca ataggatcgt atatttttga ataaatagta tccgaaaaag
     5281 tgaaaaactg gtgaattaac tcgttcattg cagctccttc atagagcttc tcaatgacaa
     5341 aatcaaggcc ttgccaaatc aatttactta aactaaagaa catattcgtc atcattttta
     5401 gaaaatctgt gccatttgcc ccaaataaca ttttgcctaa tgaaaattca ctatcagata
     5461 aataagaaga aaaggtatca atatgaaatt tcccaatctc atagatcgat tgatccacct
     5521 gcttttctgc cgcatacacc aatccaccaa caaaaagcag tccgaatcca atcgttaaaa
     5581 aaaacaaaac gcaccatttc ttttccttat gctttatata agccattgtt tcacctcatt
     5641 tctattgcat gattaacgta tcctgttctt ctacttgaga gtgacttgtt ttttccgttg
     5701 tttgtaatgc agcggtcatt tcttcaaaca aactgtgtac attgagtttt cctacacgtc
     5761 catagggatc taacatccaa cactgtcctt taatcatgtg gctcatttca gctaagttcg
     5821 cttcatttac ttccaaacgt aaatgattca aaatcttttc tcgctcactg tcttcatcaa
     5881 aagcaaagac gacaccaaaa ttcccttttt ctttatctga acctaagtca ccaataaatt
     5941 gtgtatctaa cactaattga ttcatctggg aacgccccac acgtgccatt gctgcaataa
     6001 tactacgtcc tgcgttcgaa ttattaaata cccatgcttc tgtaaaataa atcgctgttt
     6061 tcttactact atctcgtttt ccaaacattt cacaaaaacg acctaaacta atcatcaaac
     6121 acaaagactt tcgatccgca tctgtatata gttctggtcg gacattgtca tttggtaatt
     6181 tcaaatcttg gatctctaaa atcgttgttt tggcattaaa gtctaatccc gggttttctc
     6241 cataagaaaa tcctaaacgt aaaatcgaat cttctaccgt caagcgtaaa aactcaccat
     6301 attctcgaac ctctttttgt tccatttgtt ctaaatagtc gaccacatgc atcaagccaa
     6361 cttgttgtcc ggcttctttt tgaactaaaa cttcttggat tccctttaat accgcccctt
     6421 ttaaaaggtg attggtacct aacggactta actcatcaat catatcctgg gctacttgtt
     6481 tcgcatctac tcgggataaa caaacaattg gatctaatac accataatta cggggatttg
     6541 tagcatctaa cgtgacataa ctcatttttt gcaaatgttc tacaaacaaa gggtaatgtt
     6601 tctgataata cgggtcttga atgagtttcg taaaccattt gcgcttttct cctttgggat
     6661 ccacaaataa actttgtaca ttcataaacg aactatacaa aaagagcaag ccaactaaaa
     6721 aggtcttccc tttaccggtt tttccagtta ctgctacatg aggcgcatca aacaacgcac
     6781 ctttgattcc ttccgcaatg gctagaagat tcaaaaagac aaaacgattt gacgcataaa
     6841 ttaattcttc taatggtgtt ttttctcctc gagaaccacc atcccctaat gtatcttgcc
     6901 gaccaatata ccagccggat tccataccta attctgatac tgctgaaaac aaccattcgc
     6961 caactgcttc tacagttgtt cggtgcttcc aatgtaactg ccgtcccaat ggttgcccta
     7021 ataaacattt atagaacaat tctcgttgat cattttttgc acggtggata tggataatcg
     7081 tatgaagttg ttgaataacc gttgcactat tttgacgaac ttcttcaacc gtttgaccgt
     7141 atacaataaa acatgcgatc caatcgacga caggttgccc attatctact cgttgcttta
     7201 aatgttttac taatcctcga acagttaata agcgttcact aggttcttca tcattttgaa
     7261 ccaattcctt atgctctgtt ttgaaccgtt ccgatagcca attcagtttt aaaccaatac
     7321 ctccctgtcc tttcaagcgt ggatagtgaa ttttcattcg caattctgct gggaaagaaa
     7381 gatcctgagc aaattgaaat agatcacaat ttctcatatt ttcaggtgtt tccccaatag
     7441 gcaacaatgc taaataacta ctccctaact catcttctaa ttgaagaagg cctttatgtt
     7501 ccttcggtat acgtacatac gttccttgat tgtctttcag tacttcccaa ttattttgtt
     7561 cttctacttt gtgagccatt cctcgaatat attgcagacg attgagatac aacagctctt
     7621 tttttactaa gggacgaata gaaaagctat aaagtaattg tgtcacttct tttacataag
     7681 gatcactttc agaaaacaaa gtttccaaat catctacttc taatccaaat aagcgtgcta
     7741 aacttcctgc taaatctaac cagattagat ttattacatc atgaaaactc tccaccgttg
     7801 ctttttcttt gactttgatt cctaaaataa atgaaggacg atagacttga ccttctcgtt
     7861 ccaacaatgt cactgtttct tttgcataat acgcaccaat tgcttgacta tcgggagaaa
     7921 atccctttcc taatgtgtca aacttcgtcc gtaaattcat tgttcgtgga tacatcacca
     7981 actccacatc ttcataatct gctaaaagca acagtaaacg agccatcatt tcttttattt
     8041 cctgtgtctt ttttggtgtg ttttttgaaa ttgttcgtgg aaaaacatga tagtaggccc
     8101 agatacaatc ctctccattt aacaaacaat ttttcttgaa tgcttttact ggttctttta
     8161 caagcaccaa tgaatttcac ctcccaataa aaaaggaaca accagttgat tgtccctttt
     8221 tctaatcctc taaataaaca tacttattca caaaaagtaa ttcttccaat gcattgtttg
     8281 ataccttgtc ccagttataa tacacacaat caccattcat catacgaatc atataacaaa
     8341 accaatccca taaataaaaa tgcacttttt ttccatcagg ttttagtttt gccagtgcta
     8401 acacacataa ataggggagt cctatataaa aaacaactcc tgctggtgga aaaaaagaaa
     8461 tgacccaacc aataaatgaa cgtaaaaata aatattcaaa gattacgacc aacaaaaaag
     8521 ttgctgcatt gacta
//
LOCUS       DAZUKD010000120         8480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000120 DAZUKD010000000
VERSION     DAZUKD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.122"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..779
                     /locus_tag="ACG8N9_002384"
     CDS             <1..779
                     /locus_tag="ACG8N9_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897938.1"
                     /translation="LTQVQLAEGICEQATVSNIENKNMSPGINILSALCKKLNVEISD
                     ILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKVSNSDLLNQILFYQGLLD
                     HLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKI
                     LSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEI
                     ASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1052..1822)
                     /locus_tag="ACG8N9_002385"
     CDS             complement(1052..1822)
                     /locus_tag="ACG8N9_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4897939.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2040..3107
                     /gene="ulaG"
                     /locus_tag="ACG8N9_002386"
     CDS             2040..3107
                     /gene="ulaG"
                     /locus_tag="ACG8N9_002386"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4897940.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3125..3586
                     /locus_tag="ACG8N9_002387"
     CDS             3125..3586
                     /locus_tag="ACG8N9_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897941.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3606..5090
                     /locus_tag="ACG8N9_002388"
     CDS             3606..5090
                     /locus_tag="ACG8N9_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4897942.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            5133..5432
                     /locus_tag="ACG8N9_002389"
     CDS             5133..5432
                     /locus_tag="ACG8N9_002389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4897943.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            5447..6085
                     /locus_tag="ACG8N9_002390"
     CDS             5447..6085
                     /locus_tag="ACG8N9_002390"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4897944.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6090..6950
                     /locus_tag="ACG8N9_002391"
     CDS             6090..6950
                     /locus_tag="ACG8N9_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4897945.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            6940..7650
                     /locus_tag="ACG8N9_002392"
     CDS             6940..7650
                     /locus_tag="ACG8N9_002392"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4897946.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            7882..8316
                     /locus_tag="ACG8N9_002393"
     CDS             7882..8316
                     /locus_tag="ACG8N9_002393"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4897947.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2793 a         1340 c         1803 g         2544 t
ORIGIN      
        1 ggttaacaca agtacaatta gcagaaggca tttgtgaaca agcaactgtc agcaatatcg
       61 aaaacaagaa tatgtcgcca ggcatcaata tcttaagtgc gctttgcaaa aagctgaatg
      121 tcgagatctc cgatatatta gtagaaacag aagaagacaa aatcaggaaa aaaataaatg
      181 agattcaatc attgaattct gtatcaaaat accaagaatc ttataaaatt ttacaaacga
      241 tcgatcagga aaaagtaagt aattctgatc ttttaaatca aattttattt tatcaaggac
      301 tgcttgatca tctttatgcg aaaaaaccag atgcagcatt attttatttt aatcaagtgt
      361 tggaacaaac gaccgaaaca gatatccatc acttacaagc agctgcaaac gtggcaatga
      421 tctacttgtc aaaaggagaa ttagattttg cgaaagttta tgtggagcgt acacttaaaa
      481 tattgagtga gactgatttt gataatctga tggtctgcat tgtatattat gatatcgcca
      541 cctattaccg taaaatcgag gattatgata aagcaatcca attatgtgag aagggtattg
      601 agtataataa gaaacataaa tcgacttatg cattggaata cttgctttat gaaattgctt
      661 cttgccataa gcaattagga gaagatgatt atttagaaag atatatggac gcaaaaaaaa
      721 tagcgcgttt caacggaaat gattacgcgg taaaggtgat tgaaaacgac ctgaaataaa
      781 gtggttgtga aatcagtgtt ctgatttgaa taccgtttat tcgaaaaaag gaggtgtgac
      841 ttttgtcata cctccttttt tcgatattgt ttgattccta tttaggcaac atataacggt
      901 tataaaagaa gcaatagatt atgatggtat cgattggatg gacacatctt gggattcctt
      961 tatcaatttg agcgtagcgt gtttttcatt tatcgataaa tttgttattc atttttttga
     1021 taatcctatt tacatattta cgtcctggtt cttaaaatga atctacttga atggttttta
     1081 tccccgtatt tctgatctcc tctaatactt tttcagaaca aaatttatca gtgatcaaat
     1141 aatcaaagac tgaaatatca gcaatcttga actttgatgt cagtccgatt tttcgatgat
     1201 cagcgactaa aatcttacat ttgattgtct tgttgaccat catttcgttg atcgttgatt
     1261 catttaatat ctttgttgtg accccatgta ctacatcgac tccgctacag ccaatgatac
     1321 aaatgtctgc attgactgaa gaaatcgtat ttaatgcgat gtcgcccact agtgcttctt
     1381 ttggaaagcg catctctcca ccagttaaaa tataactaca attatctttg tgcggagagt
     1441 aattgatttt cacgttgttt gtaatgactg tcaaatggtt agattgaaga aaatccagtg
     1501 ttttcaacgc agtcgagcta gaattgataa acagcgtatc ataatcgtta atgtatttac
     1561 tagcttctct ggcaatcgct tgtttaatcc tttcaggacc agattgatca aaatcagtct
     1621 ggtttttgta attataagaa caataaccgt aattgcgtat gatctcgttt ttttcttcta
     1681 ataaatttaa atctcttcgt atcgtactaa tagatacatc aagggattca gctaattcga
     1741 aagttgatag ttgttcagtc tgctgaagca gttccaaaat ttctttttga cgtttttcaa
     1801 tattgacaag tgagtttttc atgacttttg cctcctgagg gacatcttga tccatggatt
     1861 tatttgacta gttgttcatt taaacattca tatctgtctt tattatactg aaacaatcat
     1921 tgattactga aagaaaatca tataaaaaac aaacggtcat ataaaagggt ttccatggat
     1981 gctgatacac attaatattt acttgtaagt tggaacaacg atatgggggg agcaagcaaa
     2041 tgagtaaaac tgtcaatgaa gtaacaaaag aaaagtggtt agtagaaaca tttcctgagt
     2101 ggggcacata cttgaatgaa gaaatcgaaa cagaagtagt ggatcctgga acttttgcta
     2161 tgtggtggct gggctgtacg ggaatttggc tgaaaagtca tgaaggaacg aatattttat
     2221 gtgatctatg gtgtgggacg ggaaaacgta gtcatgggaa tggcatgatg aaaaaaggcc
     2281 atcagatgat gcgtatgagt ggcgttcaaa atatgcagcc taatttacgt actcagcctt
     2341 tcgtaatcga tccttttgcg ataaaaaatg tagacgcttt aatcgttaca catatccatt
     2401 ccgatcatct agatatccat acagccgcag cagttcatca aaattgtcct gaagcaacat
     2461 tcattgggcc aaaagaagtg gttcgcacgt ggcaaaaatg gggaattcct gaagaaaaaa
     2521 cgattacagt aaagccaggt gatgcagtca aaatcaaaga catcaatgta gtagctttag
     2581 aagcgtttga tcggacagcg ttggtcactt gtgaagatcc agaagaggta ttaaaaggaa
     2641 aaatgcctca ggagatggat caaattgcag taaactattt attcgaaaca tcaggaggga
     2701 atctttatca tgcaggagat tcacattact ccaatttatt tgccaaacat ggtaatgaac
     2761 acaaaatcga tgtctgttta ggggcctatg gtgaaaaccc acgagggata acagacaaag
     2821 ttacttctgt tgatatgctg cgcatggcgg agtcattgaa tgcaaaagta gtgattcctg
     2881 tccactacga tatttggtct aattttatgg ctgattcaaa agagatcact gaaatttgga
     2941 aattcaaaaa ggatcgcctg aagtatggat ttaaacctta tatctggcaa gtaggcggga
     3001 aattcatcta tccgacaaac aaagatgatc ttgagtttaa tttttatcga ggatttgaag
     3061 atgtcttctc gattgaaaat gatacaccgt ttccgtcgtt tttataagaa agaaggggag
     3121 tttcttgctg aaatactttt ttgaccatca attgattcat tacccagata aagaaatcac
     3181 ggattggcgt gaagctatcg ccgaaagctg tcttgtattg atcgataagg aaattattga
     3241 tgaaacgtat gtggaggaga tcatccattg tgtagaaaaa tacggtcctt atattgtgat
     3301 cgcacctcaa gtagcaatgc cgcattcttc agaaaaaagt gatggtgttt ttggaacagc
     3361 tatttcattt accaagttga aacagccaat ccgttttgaa agtgaagggg aggatgagga
     3421 gaaaacggct agtttgtttt ttacacttgc agcaaaagat ccagaagaac atctagaaaa
     3481 tatccaaaaa ttatccgaat tattaatgac agatggattg gttgagcgtc tcatctctac
     3541 gaactccatc gatgattacc aagttgtgat ggcagaatat aattaattga tagaggagaa
     3601 aaaagatggg ggaatttttg ttaagcattt ggacgtattt tgcaacaaat atcttgacgc
     3661 agccagcata cctgattggt tttatcgtgt tattaggcta tattttgctg aaacgcccgc
     3721 ttcatgaatg catcgctgga ttcttaaagg caacggtcgg ttactttatt ttgactgttg
     3781 gctctggtgg tttggtcaat aattttcggc cgattttggt tggactaaag gaacggttca
     3841 atttggatgc catggttaca gatccttatt ttggacaaaa tgctgtggat gctggattaa
     3901 tggagacatt tggccgtacc tttggagatg tgatgatttt attactgatt gcatttgttt
     3961 taaatatctt attagtccgt tttcagaaat atacgaaact gcgttccgtt ttcactaccg
     4021 gaaacgttca ggttcaacaa gcagctaccg ctttttggat tttactgttt tgctttcctg
     4081 atctaggtag gatacaagta ttgattttta tgggactgat tttaggttgt tattgggcag
     4141 tagcttctaa cttaacagta ggaatcacgc aggaattgac agaaggtgcc ggatttgcag
     4201 tggcacatca gcaaatgttc ggaatcttta tatttgcaaa attagctgag tggatgaaaa
     4261 agcgggatga aaagaaaaat agatcaatca agcaagacaa aaaaatcgaa gatatcaaat
     4321 taccaggatt cttgtcgatt ttcaatgaga atatggttgc tacttcgtta ctcatgctat
     4381 ttttctttgg gatcattctg attgtactag gcaaagatta tttgattcaa gcgcaattca
     4441 tgcaagaagg acagagtttc ttattctaca ttatgacgac ttctttaaat tttgcggtat
     4501 atctggcgat cttgcaatta ggtgttcgga cattcgtcga tgaactaact caatctttcc
     4561 aaggaatctc aaacacaatt ttaccaggag ctgttccagg aattgacgta gctgctactt
     4621 ttggctttgg ttcgccaaat gcggtaacga ttgggttctt gtttggtgct ttgggacagt
     4681 ttttgatgat tggtttactg attttattga aatcaccaac gatcgtcata gccggtttca
     4741 ttccgttgtt ctttgataat gccgttatag cagtttatgc caataaccga ggtggattca
     4801 aagccgcttg cctgttcccg tttctttcag gaatcataca agtaggcggt tcagcattat
     4861 tcgctacatg gattgggatg tcaacatatg gtggttatat cgggatgttc gactgggcaa
     4921 cagcttggcc attgtttaca gtaatcatga aagtcctagg ttatgctgga attgccgtag
     4981 ttgtcattgg tttattggct attccgcagt tacaatatcg gaaaaatcct gagggttatt
     5041 ttttgattgt agaagactat gacacatatg cagaaaagca gagagtataa aagaaaatct
     5101 gattagacaa cactcataat ggagggatta agatgagaat tttagtatcg tgtgcaaatg
     5161 gatcaggaac aagcttgatg atgatgagaa gtgtggaaaa agcaatgaaa gaactaaacg
     5221 taccaattac caaaatccat cattgtgcca tttcagaggg aaaaagttca gctggccaat
     5281 acgatgtagt ttttacgcca gtcaattttt tgaatatgtt tactcaggca gagaaaaaag
     5341 aagttacagt tatcggtatc aaaaatgtga tgtcagccaa agaaatcagt gaaaaatttg
     5401 ctgaaacaaa gtggtaccaa aaaaacaagt aaaggaagga actaagatga gcaggccgaa
     5461 tttgcaaatt gcgttagatc acaatgactt agaacatgca ttaggagatg ttatgaaagt
     5521 tggtgatgta gtagatatta ttgaagtggg cacaattctt tgcttacagg aaggacaaaa
     5581 agcgattcgc tgtattcgca gtatgttccc agataaaaaa ctagtcgctg atacgaaatg
     5641 tgcagacgct ggaggaacag tggcgagtaa tgttgcaaaa gcaggagcag actggatgac
     5701 agtcatttgt tgtgcaacga ttccaactat ggaggcagca caaaaagaaa taggtgaatt
     5761 gcaagtagaa ctctacggca attggacttt tgaacaagcc atggattggc ataacatcgg
     5821 tatccggcag gtaatctacc atcaaagccg tgatgcgctg ttatctggtg aaacttgggg
     5881 tgaaaaagat ttatcgaaga tcaaaaaact gattgaacta ggctttaatg tttctgtaac
     5941 tggtggatta aatccacaca cattacactt atttgaaggg atcgatgtgt atacctttat
     6001 cactggtcgt ggtattacag cagcaaatga tccgatgaag gccgcacaaa acttcaaaga
     6061 tgagatcatt cgtatctggg ggtaaggaaa tggctcaaat tgggatttat gaaaaagcat
     6121 tacccaaaga tatttcatgg aaagaacgtt tttcccttgt aaaagaaatg gacttcgatt
     6181 ttatcgagat gtcgattgat gagacggatg agcggttagc acgattagat tggtctgaag
     6241 aaaagatagc tgagctgaga gaagagatgt tcagctccgg tgtacgtatc cattcgattt
     6301 gtttaagtgc gcatcggcga tttccgtttg gttctgctga tccagaaaag aaaaaagcag
     6361 cgaaacaact aatgaaaaaa gcaatccatt tggcgcataa tctaagcgtc aaagtaatcc
     6421 aaatagctgg atacgatgtt tattatgaag agaaatcaat gaattccaga gaagacttta
     6481 tcgaaggaat aaaagaaagc gtaaaggaag cttctaaata tggcatcatt ctttccgtgg
     6541 aaatcatgga tgatccattt atgaactcca tttcaaaatt tttagaaatc aaagaacaga
     6601 ttcattcccc ttttctacaa gtatatccag acctaggcaa tttgtccgca tggccggaaa
     6661 ataatccggc tcgagaactt gagaaaggga tcgattatat cactgctatc cacttaaaag
     6721 atacttaccc tgtaacaaaa gaatattcag gccagtttcg tgatgttcct tttggtagcg
     6781 gttgtgttga ttttcttgga ttattaagac atttgaaacg tctagattat gatggaacat
     6841 ttctgattga aatgtggagt gaaaaaagcc aagactttcg aagagaaatc cagcaggcaa
     6901 aagactactt aagttctaaa ttgaaggagg ctggctatga tgtcttgtga agaaaaaatt
     6961 gcagcaatga agcaacgtgt ccttgaagca aatctggcat tgccgcgata cggattagtc
     7021 aaattgactt ggggcaatgt gagtgaagtg gaccgtgagc taggagtcat cgtcattaag
     7081 ccaagcggtg tcagatatga atgcatgcaa gcagatcaaa tggtggtaac agatttgtcg
     7141 ggaaatataa tagaagaaga tagcttaaaa ccatcttcag atctccccac acatgttgtt
     7201 ttgtatcaaa cttttgaaga tataactgct attactcata ctcattccac gcactccgtc
     7261 atgtgggcgc aagcaggacg cgatttgcca gcttatggca cgactcatgc tgatgctttt
     7321 tatggtaagg ttccttgtac acgccaatta acgaaagaag aagtaagaga agcttacgaa
     7381 gttcatacag ggaatgtgat tgtcgagacg tttaaggaaa gaaaattaga tccaaacgaa
     7441 gtccctggag tacttgtcta tggacatgga ccgttcacat ggggggattc acctatgaaa
     7501 gcagtagaaa acagtttgat cttagatgaa atttgtctga tggccaaaga aaacgagctg
     7561 atcaacccca atatatgtga aatcccgcaa tatttactag ataaacatta ttttcgcaaa
     7621 catggaaagt atgcatacta cgggcaatag agatgctagg gtaagggtag aaaattaaaa
     7681 gataacactt gatagagaga aacagaggtt gtgaagaaaa tttatcacaa cctttttatt
     7741 tatcaaaaag gatgcattct aaaaggaaag agagatttta aggaatcgag agaatcttta
     7801 caggagagtt atatgtgata gattaaatag tcaaagaact gtattattag agggagaatt
     7861 gtataagaaa ggaagaaaaa aatgaatatt gattccctta tgatcgatga gatttctgaa
     7921 ttatataaga gcgttggttg gacgaattac acaaaagaca ctgcaaggtt agctagagca
     7981 tttgaacaat ctgaatctct gataaagcgt aatggggaag ggaagattat cggaatcgtt
     8041 cgttggatca ctgattttgc gacgatcgca tttattcaag atatcctcat tcatccgagg
     8101 tatcagcgcc aaggaatagg caaagtgctt ttgaatgaag ctttggagaa gatcactagt
     8161 tatggaccag tccaaatcga attattgaca gatgatactg agaaaacaaa aaaattttat
     8221 gaatcagtag gatttgttca agtaaaggaa atgggtggta tttcttatgt catagatact
     8281 agaagaaaaa aggtgctttt tggtaatgac aaataacata taataacgta caaaagaaaa
     8341 aattctagct tagttcttta gaaaaacaag aggatagact tagtatcact caatctatcc
     8401 tccaatgctc agctatttta tttttttact cacctcttta tcaaatacaa ttttttgcaa
     8461 attcgaacta atataaaaaa
//
LOCUS       DAZUKD010000121         8426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000121 DAZUKD010000000
VERSION     DAZUKD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.123"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..289
                     /locus_tag="ACG8N9_002394"
     CDS             140..289
                     /locus_tag="ACG8N9_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4897948.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            300..1814
                     /gene="dltA"
                     /locus_tag="ACG8N9_002395"
     CDS             300..1814
                     /gene="dltA"
                     /locus_tag="ACG8N9_002395"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4897949.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1811..3016
                     /gene="dltB"
                     /locus_tag="ACG8N9_002396"
     CDS             1811..3016
                     /gene="dltB"
                     /locus_tag="ACG8N9_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4897950.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3061..3294
                     /gene="dltC"
                     /locus_tag="ACG8N9_002397"
     CDS             3061..3294
                     /gene="dltC"
                     /locus_tag="ACG8N9_002397"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4897951.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3297..4565
                     /gene="dltD"
                     /locus_tag="ACG8N9_002398"
     CDS             3297..4565
                     /gene="dltD"
                     /locus_tag="ACG8N9_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4897952.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4706..5788
                     /locus_tag="ACG8N9_002399"
     CDS             4706..5788
                     /locus_tag="ACG8N9_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4897953.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5788..6591
                     /locus_tag="ACG8N9_002400"
     CDS             5788..6591
                     /locus_tag="ACG8N9_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4897954.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6661..7401
                     /locus_tag="ACG8N9_002401"
     CDS             6661..7401
                     /locus_tag="ACG8N9_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4897955.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7412..8416
                     /locus_tag="ACG8N9_002402"
     CDS             7412..8416
                     /locus_tag="ACG8N9_002402"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4897956.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         2706 a         1389 c         1737 g         2594 t
ORIGIN      
        1 cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag taaaaaaacg
       61 gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt
      121 tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga attattgggg
      181 aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga tttatctgta
      241 tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg aggaagagta
      301 tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa cctgatcgag
      361 ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca tcagacgcac
      421 ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt tttggcaatt
      481 tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat gcctatttgc
      541 cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg gctaaaccat
      601 ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct gtcataacaa
      661 aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga aatagcgtaa
      721 aaggtacaga aaatttttat attattttta cttctggtac tactggagaa ccaaaaggcg
      781 tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa gactttggga
      841 tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta tcggtatttt
      901 ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa gcagttgttc
      961 aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg tgggtttcta
     1021 ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa aatgtgccgg
     1081 aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca gaaagtctac
     1141 ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa gcaactgtag
     1201 ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga ctaccgattg
     1261 gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag
     1321 gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac ctgaacaatc
     1381 ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac catacagggg
     1441 atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg gatcagcagg
     1501 tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg ctccaagaca
     1561 accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta cagcagctgg
     1621 ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg actaaatcta
     1681 tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag ttccattatg
     1741 tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta acagctgagg
     1801 tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt atttttttct
     1861 tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa aaagattacc
     1921 agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag gtaattattg
     1981 gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg tatggggcta
     2041 ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg ttattttagc
     2101 gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa aagcatcgtt
     2161 acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga tcatggaaat
     2221 aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc tgctgttctt
     2281 tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag atcagctcca
     2341 tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa atatttttgt
     2401 cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc ttcctgtagt
     2461 gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt atatgtacgt
     2521 gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag tcggtacaag
     2581 ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat catggaatat
     2641 caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg attatgtata
     2701 tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc
     2761 aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat tgacgtggta
     2821 ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg catggatccg
     2881 atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg gcttagccgt
     2941 atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat ttttagataa
     3001 actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga gaaaaggaaa
     3061 atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga agtaagaaaa
     3121 aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta
     3181 ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt cgagcgagag
     3241 gattggagca caccgaataa aatcattcaa caagcaactg cattgaaggg ttgagtatga
     3301 aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt
     3361 tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga cgtgcctctt
     3421 cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca ttatctgaaa
     3481 aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca tttcatccgt
     3541 ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga gcacccggga
     3601 cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg aaaaacaaga
     3661 aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa actgattact
     3721 ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg aagaaagtaa
     3781 cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta aaagagaatg
     3841 atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta cctgaatctt
     3901 tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta ttcagcaata
     3961 ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat
     4021 accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa acgacgaata
     4081 atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac gagccagagc
     4141 tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct gattttcagc
     4201 tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc ccaccggtta
     4261 acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga tttgccaaaa
     4321 agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt gtcaatcaag
     4381 cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc tggttggctg
     4441 cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt aagtatcatc
     4501 ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa ctacaattaa
     4561 aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat attctgaata
     4621 aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat aatcacaata
     4681 tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac ttattgattt
     4741 aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat tcagagaata
     4801 tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga ttgccaaaca
     4861 tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag ttggttttat
     4921 ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag gttggtgggg
     4981 gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg
     5041 tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata aaccttatga
     5101 aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca aagagtgggg
     5161 catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga atggcagtaa
     5221 atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc tgcgagtatt
     5281 agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg atgtgcaaga
     5341 agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg atattgcttt
     5401 tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag aagcagattc
     5461 gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc cgcataaaaa
     5521 attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc aatatacagt
     5581 gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg gtgttccgtc
     5641 tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga ttcatgaaga
     5701 tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg atagtgaaac
     5761 agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc tgaatatcgg
     5821 aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct tgttttctta
     5881 tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc ttttccggaa
     5941 gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt attcgcattc
     6001 aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc aaaaagtaac
     6061 cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat caatttgatc
     6121 aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt tttaatcagc
     6181 ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac gggtgtcatg
     6241 ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat ttttgtttca
     6301 gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac aaagttcgac
     6361 tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt gaaagataga
     6421 attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct aggagcaatc
     6481 gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt
     6541 tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta gcatgtaagc
     6601 agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg ggaatgtacg
     6661 atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca agggacaaca
     6721 ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag tgctacgctg
     6781 atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct tagccattat
     6841 catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact tcatcctaag
     6901 agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat tcactttgaa
     6961 gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga agaattaaaa
     7021 attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg ttacgccatg
     7081 cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc aggttatttg
     7141 gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac ctatcttttt
     7201 gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga acttgcgaaa
     7261 tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga tttagaacaa
     7321 ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact tgctgcagtt
     7381 gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg aaaaccacga
     7441 cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc
     7501 tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc aaaatacgct
     7561 tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc gtcgcttaag
     7621 tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta tcatgcctga
     7681 tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc aagcacttca
     7741 tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca ttgatggcat
     7801 gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc atggaacaat
     7861 tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa aagacaaaat
     7921 cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac catttcttcc
     7981 agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt
     8041 cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag attggaaaaa
     8101 aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg ggaagtctcc
     8161 agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag tccatttgaa
     8221 tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg gactaggaga
     8281 tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat ctctatcaaa
     8341 aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag aagaattgct
     8401 taccttactt tattgaaaat aaaaaa
//
LOCUS       DAZUKD010000122         8364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000122 DAZUKD010000000
VERSION     DAZUKD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.124"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(232..909)
                     /gene="nth"
                     /locus_tag="ACG8N9_002403"
     CDS             complement(232..909)
                     /gene="nth"
                     /locus_tag="ACG8N9_002403"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4897957.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(916..1614)
                     /locus_tag="ACG8N9_002404"
     CDS             complement(916..1614)
                     /locus_tag="ACG8N9_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4897958.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1882..3066
                     /locus_tag="ACG8N9_002405"
     CDS             1882..3066
                     /locus_tag="ACG8N9_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4897959.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3072..3638)
                     /locus_tag="ACG8N9_002406"
     CDS             complement(3072..3638)
                     /locus_tag="ACG8N9_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4897960.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIPDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3741..4028)
                     /locus_tag="ACG8N9_002407"
     CDS             complement(3741..4028)
                     /locus_tag="ACG8N9_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4897961.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4199..5551)
                     /locus_tag="ACG8N9_002408"
     CDS             complement(4199..5551)
                     /locus_tag="ACG8N9_002408"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4897962.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(5823..6269)
                     /locus_tag="ACG8N9_002409"
     CDS             complement(5823..6269)
                     /locus_tag="ACG8N9_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4897963.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6505..7431)
                     /gene="cysK"
                     /locus_tag="ACG8N9_002410"
     CDS             complement(6505..7431)
                     /gene="cysK"
                     /locus_tag="ACG8N9_002410"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4897964.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7571..8364)
                     /locus_tag="ACG8N9_002411"
                     /pseudo
     CDS             complement(7571..8364)
                     /locus_tag="ACG8N9_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT         2269 a         1720 c         1400 g         2975 t
ORIGIN      
        1 ggaatactgg acttatttta taggtatatt ggaaggcatt attttctaca tcgggtattt
       61 tcccaataaa aataacaact tttattttta tcgcaataaa ttgtaataag ttaccatcct
      121 agctactgag tatgtacatt tactttttca tgcaaaaaag ataaacgaaa cagcttttct
      181 ccataataac aagataattc atacatttat ttgcaattaa agaaaccacg tctacaggcg
      241 tggtttcttt tttagcgcct tttcttttag acgcatccga tttttcccat cttgacatat
      301 tgatagcaaa ggacatacct cgcacttggg atttcttgcc gtgcaatgat atctaccaaa
      361 aaaaatcagt gtatgatgtg tctttaccca taattcttgt ggtactttcc gcatcagtgt
      421 ttcttctact tccatgaccg tagcatctag tttgcagatc ctcaggcgtt ttgagacacg
      481 ttccacgtgg gtatctactg cgattgctgg tatcccgaat gcatccccca atacgacatt
      541 tgctgtcttt ctgcctacac caggaagaga catcaattct tctcttgacg ttggcacttg
      601 cccatcgaaa cgttcaatca gctgctgtgc acacgctttg atgttttttg ctttatttcg
      661 atacaaacca atcgttttta ttttaagaat gatttcatcg attgaagctt ctgctaacgc
      721 atcgggtgtc ggaaaagagg caaacaaatc tggcgttgct ttattcactg atacatctgt
      781 cgcctgggca cttaagatca ctgcaatcaa taactcaaat ggattattgt gcttgagttc
      841 tccatgtgct tcgggaaaca ttccatacat cgtttctaga gcttccattg ttttttgttt
      901 gcttaacata ttccgctact cctccaacca atttttcagt gagactttcg gcaattcttc
      961 ttgttgagct gactgttcta cttctctttg aagaagctgc tgttttcttc gtttttggtc
     1021 ttcttccact tgttgttttg tacgtagatt ttttctttcc cacgatagca aaatgcggtc
     1081 aacgtaatta aaactataag cctgatttaa tacggcttcc cgcagtgcta gcttcaaaat
     1141 ttccggctga taatggtctt cttctagcca ttgtccgatc cgctggaact cgatcgaaga
     1201 aagcgggcgt ccaaattctt gttcaaataa ttgatacact gactggattt ctttttcttg
     1261 tgttttttct tcgtattttt tttcttgtgt ttttaaatac tcacctaata gatcatagat
     1321 ggggtaaaga ttatatcgat ctgctttttt accgttgaca atcgtcgttt ctatcttgat
     1381 aaatccattt gttacgagac gatccagtat ttgaaaaatc tgatcttgtt tgattcccat
     1441 atccatagca atcatctgca aatcagggaa agtatctcct tcaagctgtg ccatatgcaa
     1501 ctgtagaaca aataaaaatt cttcagattg tagaccgatc ctatgataat ttttaagtaa
     1561 aagattagat atgactgttt gtccagcgct taagtaattt tctaaattca acatgatttc
     1621 tcctccgctc aatcacagta tacgtaaaaa aaacatggga agcaagaaca ggtttctttt
     1681 ctatttactt cactgatcgg tcagatttac aatgtcgtct acttgatttt tgtttgttca
     1741 tgtttttgat gcgtatcttc acttttcgga ataaataaat taagtagatt tcgctgttcc
     1801 ttagaatgtt gacaaggaaa caaaattact atacactatt tttgtttcca agttaacatt
     1861 attgcataaa ggagttttta catgtacagt ttcacaaata aacattctaa agtttttgga
     1921 tacgtctctg tcttattcac tggattagga tttactatcg tcagtcctgt acttccgttt
     1981 ttaacgctgc cttattctca ctccatacat cagcaagctt tctatatcac tttgctcatg
     2041 tctgtttatg ctctagctgc cttcttatct gcccccattc tcggtagctt aagtgatcat
     2101 tttgggcgtc atccgatcct tattatcagt ttattaggat caagtatcgg atatttgctt
     2161 tttggtttag gaaattcgat atggatgttg ttccttggtc gtatcattga aggattgacg
     2221 gcaggagaaa tctccacctt atatgcttac tttgccgaca tcacggaacc taatgaaaga
     2281 accaaagttt ttggatggat gggtgcttta gtcggtatag gaacaacgct gggtcctatt
     2341 atagggggtc tattagctga gttggggaat tctgttccta tatttattgg agcactcttt
     2401 actttcttaa atgccgttta tggctacaca tttatgctag aaagccttcc aataaaaaaa
     2461 cgttcagtag atttaagttt ctcccatgta agaccttttc accaattaca acaattattc
     2521 aagatttcat ccgttattcc tttattgaca gcaggttttg ctgtttggtt ggcagctggc
     2581 tctcttcaat ctatcttttc tcaattttcg attgacgcct ttcagtggaa ggctgggttg
     2641 gttggtttaa gcttctcttt gattggaatc ttggatactg tctcgcaatt gttcattatg
     2701 cctcgattgc tcaaaaaatt ttcagaacag cagatcacaa gaatagggat gttcagtgaa
     2761 atcctcgcct atctattcat cacattatct ggaattcttc tactgccgat tttgttttta
     2821 tttgggatta tctgttatgg tttcggtgat tctattttta ctcctgtatt caatggccaa
     2881 ctatccaatt ctgtctcaga taaccagcaa ggtcttgtaa tgggcggtac ccaaagtata
     2941 caatctctat cgcgcgtcat cggcccgcta atcgcaggac agctttatgc tgcagcacca
     3001 tttcttccga cggtattcgg atttttgctt atgatttttg cctattatca ctacaataaa
     3061 aaatagtagc ttcatttgct acttcgtata aattctctgt atcgaccgat aaaacgaaat
     3121 actgtgtcaa attcttcttt tgacatattt tcaaatacag tagcatctcg ctgtacccat
     3181 ttttgatgga gttgctcgtg tctattcctt attttttgtc cgattggcgt caattgaaaa
     3241 tagatttctt ttttattctc ttcgctttga taagtgttta tgatccctct agctatcagt
     3301 ttttttgtta gcttactgat ggctcctcta gtcatgtaac tagcattcgc taattttgtc
     3361 acgttaggat cagggatttt cccgatatgt tcaatcactt ctagttcatt tagagtaaat
     3421 cctgcaaatt ctttttccat ttctggttta ttacgatgaa taatctcatt agctaaatcc
     3481 agatattctt ctattagcag ttgtctttta tccatttcta caccttcttt ttctttcatt
     3541 atattgtttt tttgtttcct attcaacaaa gataaaaata ttttgtagga aaacccaatc
     3601 tgaaaccaag aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag
     3661 atagtaaaga gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc
     3721 ggccttttca aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct
     3781 tcatatgtgt actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg
     3841 aaaggaaaac gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc
     3901 tcttgcgttt cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt
     3961 aatttcttcg aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct
     4021 ttattcatct tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat
     4081 gtaacagctt tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg
     4141 tacttttcac aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt
     4201 attgtgcagt ttcgatttcg cctagcgctt cttgtgcaag taggttcaag taattccatg
     4261 ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt
     4321 tattttgaac agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga
     4381 actgagcacc tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt
     4441 cagtagttgg catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta
     4501 gatcgttcaa agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata
     4561 ggtaaagtcc tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat
     4621 ttcccacaag taaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc
     4681 cacttggatt gtagttgact actgcagagt ctccagctgc aaaaatgtct ggatcacttg
     4741 tctgcatgta ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga
     4801 tcagttttgt atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat
     4861 ctgttggtgt agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat
     4921 tttcgcctag cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga
     4981 atggtttatc tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt
     5041 ttccagattc gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta
     5101 ctacacgttt tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc
     5161 ataggagtac atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag
     5221 ttgtcatcac taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg
     5281 ccgttactgt tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg
     5341 ctcctaatga agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta
     5401 ctccgccgac ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat
     5461 atactgtcac ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg
     5521 catgagtaca gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc
     5581 ttcgacaaaa ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc
     5641 acaaaggttt gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaagat
     5701 tcttattgtg ataatattca ctttgatatc taagattgtg aataaaagac aaaaaaataa
     5761 aaaagctagc gtttttttac acagctagct ttttttaggt ataacactta tagaaactgt
     5821 ttttacattt gcgccttttt tgctgactct ttacaatccg gacagagtcc atacaattcc
     5881 aaccgatgtt cattaatctc aaaatcagtc agtctgctcg cagccatttc tatatcttca
     5941 agccctgggt aatgaaaatc aacaatctta ccgcagtttt cacaaattac atgataatga
     6001 cgctttgaac tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc ttgaacaaat
     6061 ccaatcgaag taaataagcg aaggttattg taaacagtcg caacactcat atttggaaac
     6121 cgatgttcaa gtgctttata aatctcatca gccgttggat gggaatgatg ttcaatcaaa
     6181 tattctaaaa ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt taacttttcg
     6241 ataacctctt cgactaactt attttccatg taaacacccc acgttgttat tctcgtctta
     6301 tcatactatt tttacatttt aaaatccact taacatgaaa agaaaattca attttattgt
     6361 attttaatga aaaaagacgt aaaacagtcg cgttcactat tttacgtctc tattcaaata
     6421 atttttctca tcataaattt ttgtgtgttc ttttcatctt taaaagtttt ttacataaac
     6481 ctatctggag aaaaaccaag tgccttatat ctcttggtaa agggctgtag ataaataacg
     6541 ttcaccattg tcgggaacca cagctaatac cttcttgcct tttcctaatt cttttgcgac
     6601 ttttagcgcg gcagcaattg cagcgccaga tgagatgcct actaaaatcc cttcttttcg
     6661 cccaacttct ctagctgttt ccatcgcttc gtctccacta atagaaagga ctttatcata
     6721 cacagaagta tccagtgttt taggaacaaa acctgtaccg attccttgga ttttgtgagg
     6781 tcccgcttct ttcccttcta aaatagcaga ttctgctggt tctaccccga ttaattcgat
     6841 tttcggatac acacgtttta attctcggcc agcgccagtg atcgttcctc ctgtaccgat
     6901 acctgcaaca aacgcgtcca agccatttac tccaaatgct tgatgtatct caggaccagt
     6961 cgtcttctca tgcacaagag gatttgcttc attttcaaat tgtaatggta aaaagtagcc
     7021 gttttctttt gccaaacgtt ctgcttctct gattgatcca gaaataccat ccgcaccagg
     7081 agtcaaaatc aactctgcgc cgtacccttt catcaataaa cgtctttcga tactcatcgt
     7141 ctcaggcatc acgatgatta ctttatatcc ttttgcaact cctaccatcg acaatccgat
     7201 tcctgtattt ccagaagtag gttcaatgat agtatcacct ggtttcagta gaccatcgtg
     7261 ttctgctttt tctatcatac ttaaagcaat acgatctttt acacttcctc ctggattaaa
     7321 aaactcaagc tttacaaaaa catctgctga atcttctggt acgattttat ttaatttgac
     7381 gattggcgtt ttgccaatca attcggtgac tgagttatag atagctgtca tcttcaattc
     7441 ctccttgtct ttgctcttct tacttatcat aatacaatct gttcatttat ggcgagtgat
     7501 tcgtctttga ctagcaaaaa aaagacaaag atataccata tatggtagtt tctttgcctt
     7561 tttatctaaa tcaaacgttt agttcttgac caactaataa agaataactg gacatagaca
     7621 tgccattttt cgccattaga tcatcaacag acatgccaaa tttttgtgca atatcccata
     7681 gcgtatcacc tgacacgact gtatatgtct gaccattatt tgtatttaca tctgttgttg
     7741 aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt tgtacttgtg gtagccgaac
     7801 ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt aatcaatgag ttcaatttgg
     7861 ccccataatt tggatcggta gcataacgac ctgtcaagta cgctgttgca tcataaaaac
     7921 ttgttgtatt gcttttccac acgccagcat aatgataatc gcccgtggaa aaactggttg
     7981 aacgaagaac attcgcatgg tcttggaagg attcccaata agaagaatat tttcggaatg
     8041 gttcattcat cgttacccat tgtccgttac atattctttg gttgccatat aaacgacctg
     8101 tccgttatag cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga
     8161 agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt
     8221 acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc
     8281 aactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat
     8341 cggcccaact aaaagactcg ttcc
//
LOCUS       DAZUKD010000123         8117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000123 DAZUKD010000000
VERSION     DAZUKD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.126"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..803
                     /gene="purR"
                     /locus_tag="ACG8N9_002412"
     CDS             <1..803
                     /gene="purR"
                     /locus_tag="ACG8N9_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4897965.1"
                     /translation="ERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDLGIIKKT
                     FKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGYVYLSDL
                     LGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVRRDSKIT
                     EGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSLIEEFES
                     ELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1306..2679
                     /gene="glmU"
                     /locus_tag="ACG8N9_002413"
     CDS             1306..2679
                     /gene="glmU"
                     /locus_tag="ACG8N9_002413"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4897966.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2773..3744
                     /locus_tag="ACG8N9_002414"
     CDS             2773..3744
                     /locus_tag="ACG8N9_002414"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4897967.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3750..3852
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3918..4487
                     /gene="thiT"
                     /locus_tag="ACG8N9_002415"
     CDS             3918..4487
                     /gene="thiT"
                     /locus_tag="ACG8N9_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4897968.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4568..5341)
                     /locus_tag="ACG8N9_002416"
     CDS             complement(4568..5341)
                     /locus_tag="ACG8N9_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897969.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5608..7308
                     /locus_tag="ACG8N9_002417"
     CDS             5608..7308
                     /locus_tag="ACG8N9_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4897970.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7278..7496
                     /locus_tag="ACG8N9_002418"
     CDS             7278..7496
                     /locus_tag="ACG8N9_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4897971.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7516..>8117
                     /locus_tag="ACG8N9_002419"
     CDS             7516..>8117
                     /locus_tag="ACG8N9_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4897972.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFL"
BASE COUNT         2721 a         1407 c         1703 g         2286 t
ORIGIN      
        1 gtgaacgtct agttgacatg acacagtatt tattgaatca cccgcatgaa ttgatttcct
       61 taacaagctt tgccgaacgt tatgaatctg ctaaatcttc tatcagtgaa gatttgggaa
      121 tcatcaagaa aacatttaaa gaacgagggt atggaacctt ggaaacaatc ccgggtgctg
      181 caggtggtgt attgttcatc cctgaaattt catatgaaga ggcgaaaaaa tatattgaat
      241 ctctagcaga acgtctatca gaacaagacc gtctgcttcc aggaggatat gtttatctct
      301 ctgatttgtt aggagaacct gaactgctgc gtcaagttgg aagaatcatt gcttctaaat
      361 accttggtga aaaaatcgat gctgtaatga ctgtagccac aaaaggcgta cctattgccc
      421 aagctgtgtc ctattatctg aatgcgccat tcgttattgt acgccgggac tcaaaaataa
      481 cagaaggatc aacagtaagt gtcaactatg tgtctggatc ttctgaaaga attgaaaaga
      541 tggaactgtc taagagaagt ttgaaacgtg gttcacgtgt ccttgtagtg gatgatttca
      601 tgaaaggcgg cggaacagtc aacggaatga agagcttgat tgaagaattc gaatcagaac
      661 ttgttggcat cactgtattt gccgaatcaa aattcaacgg tcatcgcgcc atcgatgatt
      721 atacgtcact gctttatgtc aaagatgtag atactcaaac gaaaaatatt acggttgtgc
      781 caggaaatta tttttcagaa taagaggttg tgaaaaagcc gcttagtttc gagtagtaag
      841 aaaaattttg tgaaaatggc tttccacatt ttttcataaa tttggcttaa taccgagaaa
      901 ctggcttttg aacaccgttt atctagaggt tgtgaaatca gcgtcctgtc ctgagtatta
      961 gctcaataaa cggaaaaatt gctcctcata ttttgccgtt tattgagcta ataccgaagg
     1021 tcagctgatt gaaaaacgtt taatgaagag gttgtaagcc taccgtttaa ctctaagtca
     1081 caagaaacga tttcaaaaaa cagcttttca tgttttatga gtagtgtgac ttgtgaccga
     1141 ggagttggca ggagagcacc ggttaccaaa tagataggcc cgttatggtc tatctatttt
     1201 tttatccaga aaacgctggg ataaaagaaa atcacttgtt ttgttgaaaa aatgggtata
     1261 ctgggataaa ctgaaatcag caaactatat ttaggagtga cgcagttgga cgcacgttat
     1321 gcaattattt tggcggcagg gaaaggtacc cgcatgaagt caaaattata taaagtttta
     1381 catcccgttt ctggtaaacc tatggtagaa catatcatta atcgggtcag tgaaacgaaa
     1441 ccagatgaag tgattacgat cgttggacac ggagcagaac aagttaaagc acaactagga
     1501 gaacgcagta aatatgcgtt acaagcagaa caattgggaa caggacatgc tgttttgcaa
     1561 gctgcttctt tcttggaagg aaaaaaagga acaacgttag ttatcagtgg cgatacacca
     1621 ttattgacta ctgaaacgct gaacaatctt tttgaatatc accaaggaaa aaatgcaagt
     1681 gcaacgatct tgacggcaca agcagaaaac ccaacaggat atggacgaat catccgtgac
     1741 catattggga tcgtagaaaa aatcgtggaa caaaaagatg caacaccaga agaagctctt
     1801 gtacaagaaa tcaatacagg aacttactgt tttgacaacg aagctttgtt cgatgcgtta
     1861 agcaaagttg gaacaaacaa tgctcaaggt gaatactatc taacagatat catcgaaatc
     1921 ttaaaagaag aaggccatac agttgctgct tatcaaacgg atgattttga agaatcaatg
     1981 ggagtcaatg atcggatcgc gttagctaaa gcaaatgaga tcatgagaaa gcgaattaac
     2041 cagatgcata tggtcaatgg cgtaagcttt gtcgattcag caacgacata catcgatgca
     2101 ggcgtagaaa tcggtccaga cacattgatc gaagcaggtg tacaaatcca aggaaataca
     2161 gtcattggtt ctgattgtgt catcgggtca cattcaaaaa tcgtagacag taggattgag
     2221 gatcacgtcg tcattgaaaa ctcagtcatt gaaagcagcc atgtcaagaa acatgcagat
     2281 gtgggacctt atgcacattt acgaccaaaa gctgaaattg gcgaaaacgt ccatatcgga
     2341 aactttgtgg aagtgaaaaa tgctcaaatc ggcaaaggaa ctaaagtcgg tcacttgact
     2401 tatgttggtg atgctacgct gggagaagaa atcaatgtcg gatgtggtgt tgtcttcgtc
     2461 aactatgatg gaaagaacaa acatcatacg actgtcggcg accacagctt tattggttct
     2521 agcacgaata tcatcggacc tgtcgaggta gctaaaaaca gttcgattgc tgccgggtca
     2581 acaattacag acaatattcc agaatatgcg ctggcaattg cgagagcaag acaagtcaat
     2641 aaagaaggat atgcaaaaaa actcccttat ctaaactaag acttttgcaa aatcacttga
     2701 tttatttaaa aaatatgact actatttatt aggtagaatt ctagagttgt gaaaaagaaa
     2761 gtggaggtcc ccatgtcaaa acattacttt gacccaagat taaaaatttt tgcgttaaat
     2821 tccaatcgcc cattagcaga gaaaattgca gcagcagtcg gcgtcgagtt aggaaaatca
     2881 tctgttactc aatttagtga tggagaaatt caagtaaaca ttgaagaaag tattcgtgga
     2941 tcgcatgtct atgttattca atcaacgagc agcccagtca atgataactt gatggagttg
     3001 ctgatcatga ttgacgcact gaaacgagca agtgcaaaaa caatcaacgt agtcatgcct
     3061 tattatggct atgcgcgtca agaccgtaaa gcacgtgcac gggaaccaat cacagcgaaa
     3121 ttagtagcaa acatgattca aaaagctggt gcaacaagaa tgctgacttt ggaccttcat
     3181 gcagtccaaa tccaaggttt ctttgatatt ccagtcgatc atttgatggg tgcgccactt
     3241 attgccaatt atttcttaga aaaaggaatc aaaggcgatg atgttgtcgt tgtttcacca
     3301 gaccatggtg gtgtcacacg ggcacgtaaa ttagcggaat ttttgaaatc tccgattgcg
     3361 atcatagaca aacgccgtcc aaaagcaaat gttgcagaag taatgaatat cattggacaa
     3421 gtcaaaggca aaacttgtgt actgatcgat gatatgatcg atacagcagg tacgatcaca
     3481 ttggctgcca atgctttgaa agaagctggt gcaactagtg tgtatgcttc atgtacacat
     3541 ccagtattat ccggccctgc tttacaaaga atagaagatt cagctatcga gcgtctagtt
     3601 gttacagact cgatctatct tccagaagat cgtaaaatcg acaaaatcga tgaagtaagt
     3661 gttggcggac taatgggaga tgcaatcaaa cgtatccatg aaaacaaacc agttagtcca
     3721 ttgtttgaaa caaaaaataa ataataaata aactcacaaa ggggagtcca attgggctga
     3781 gattgaatct atttctaaac ccttcgtacc tgtatcggtt atgcgagcgt aggaattgtg
     3841 aataaacagc tgtttgacta ttttcgaaag ctgcttcctc ctttgtgact tttttattca
     3901 aaggaggaag tttttttatg ggaaaaaata gagtttgggt agaaggaacg attgttgcag
     3961 cgttagcgat ggtgttgtcg ttgattccga tccagatcgg cagcagtttt tctatttcat
     4021 tagggcagat tccattgact ctttttgcgt taagaagagg gacaaaggca gggatgctgg
     4081 caggtttcat ttggggcatc ctacattttc cgttgggaca agtttattat ttaagtgtcg
     4141 ttcaagtgtt gattgagtac ccgattgcct atacttttgc aggcgcagca gggctcatgg
     4201 cttcaaatgt ccaaaaaggc tatgtagacg ataatacgaa acaaattaga aaaagcatcg
     4261 tgtttggtgc attgattggc gcactttcca gatatttttg gcattttatt gcaggagtgg
     4321 ttttctgggg agcgtatgca ctatggggga tgaatccttg gctcttttct ttcgtaatga
     4381 atggtgccag cgggttgagt acagctattg taacgatcat cgttttatta gggatccaac
     4441 aaacgactcc ttcgattttt gtgccagata aacgacagga gctgtaaata aaaaagaagc
     4501 aagaaacttg agacagaatc atgtcctagt ttcttgtttc ctttttattt tttgataaga
     4561 aatagatcta cttcaatggc agataagaaa gatcggcatc ttctaccaat tgccagtgtc
     4621 gatcggtgac agagtagatt aaagtagcga tgatttcctc ttctcgatac cgggtgcctt
     4681 ttaatttcca aatataatct tcaatcacat agattgggat acgatattta ccatcgatca
     4741 aaccaacttg atcaaaagaa aaaatgatcc cttcctgcat caaattacgc atttctttca
     4801 ataattccac actgattttt tcaacaggtg tttctttttt gaaaaaagat ttttttacat
     4861 tgatcttatc tgacaagatc gaacttaatt tactagcaaa aatcctataa aattcatcta
     4921 aatagcgata ataaatccga ttcagctgat cattaagatc gaagtataca aaattattct
     4981 gtagtttata aaaaaacggc gaatgaagat gtgttttcat atgaccaaaa tataataatt
     5041 ctgaaatttc taaaggtgtc aattctttca gcatcgttaa atcagtaaag tcgatccact
     5101 tcaattcatc gaatccacgc ctgttacgag aagaggaaaa gaaacgctga atattttctg
     5161 taccacggat tactttcagc cctgtatggg attcatattc tccagcagaa gcagatggat
     5221 caagcaagag caagttttga ggaaaatgca cgatactgtt tgcaaagtca gtagcagtca
     5281 gcccttttgt taaaacagca ttacttgtgg tatcaatatg tacataaact gactcagtca
     5341 tcatgttcct cctcttattt cctcttaatc tcttttattt tacaacattt gcattaaaaa
     5401 agcttgtttc ttttatttta aaattccatt acaaaatcac gttttcattg aaaacactct
     5461 cattttcaat taatttttgt gatttttgta tttttttatt taaaggaggt tttatgaatt
     5521 gttttcttat ggaatctaca aagtcgaata cgagactcat gaccttttta tgacgtactg
     5581 gtatacttga agaaaaagga gagcgcgatg gagataaaaa aagtttactt ttcacagaaa
     5641 atgtcgctgt attcgcctat ccagcaagca aaacaacccc ttccattttt tcaaagtcat
     5701 caagaaagta gagacgcttt ttggcgttct gcaaaaaagc aatgtgcttt taaagcagta
     5761 tcgaaaaaat ataccgtcta ttttactatt gctaaaagta gaggagatgt tatttttaat
     5821 aatctgttga ttgaaggtga aacttttgaa tggaaaaaat tttttcggta tatggaagct
     5881 tgcgctcgtt tttttattaa agaaaactgt tgttttttat ttcaatctcc tctatcagaa
     5941 gaatggttgc gtatttttca taaaaacggc tatcaaggaa ccacacaatt aaataaaaag
     6001 ctggtctatc atacagcact tgttttaggt ggaggaggag cgcacggtgc ttatcagatt
     6061 ggtgtgtggc aggcgctgaa ggaacatgat atcaactttg agattatcac aggtacctcg
     6121 gtaggtgcgt tgaacggcgc attgatttta caaggagata tgaaaaaagc tgtgaattta
     6181 tggaaaaagc tgtcgacaag acaagtgctt gcgttaccag aaatggctgc agttgaagac
     6241 cttcgtgaac gcttctatcg agaaagacgc caaatgacga aaacagcact aattgaaggc
     6301 ggggtttctt ctgctccatt ggaaaagatg gtcaaagaca atttggattt gtccttactc
     6361 aaacataatc ccaaaatacg tttatatacg gtgtctacta aactgccaga actagcggaa
     6421 gtagtgactg atatccaaca gacaaaaaca gaagaaatac cagattggat actcgcctct
     6481 gcttcatttt atcccgctat ggcttatcga aagatcggaa aaaataaata tgcagacggt
     6541 gggtatcgca acaaaatacc tattgatatt gcgattaaca aaggagcaac agaggcattt
     6601 gtcgtagatg tgcaagggcc aggcccagcg aaaaaaattc gtatgccaga catttttatc
     6661 cattggaaat gtcaaacatt gtggacacta ggcagctttc tgctttttga tagccaaagg
     6721 aaccaattaa atttacaatt aggctatctg gaaatgaaaa aacgattagg ctattactat
     6781 gggaactggt atacatttca ttctgttaaa cctgcaagaa catactggcg aggatttttg
     6841 tcttacttaa caaaagaaat tcagttagaa ttatcttttt tcaaaagcat aaaattttgg
     6901 caaaaactgc gtaatttata taagaacagg gtcgtaccag agacatgcgg attggcgatg
     6961 ctagaacttt tagcaaaaaa acatttttta ctgcccaatg aaatctatca agtagacatg
     7021 atgattcaaa tgatctgcca acaagatata aaaagtatgc cggatgattt attggcacaa
     7081 attggtcaat taagtactga agaatggcga aggtatcgaa aataccagca aaaaatacaa
     7141 aatgagcgca cgaaaactgt ttattttgat tatctgctac aaggaaaacg caaggatcgt
     7201 ttgcagcatg aattgcttaa gcaaccagtt gacacattac tgatcctata tttatattat
     7261 ctcaaggagg aacaaccatg gcacaagaat tttcatatga aatcgtagaa gaaatcgcta
     7321 tattgtcaga aaacaacaag gggtggcgta aagaactgaa cctagtcagc tggaatggtc
     7381 gcccgccaaa attcgatctt cgagattggg cgccagacca tgaaaaaatg gggaaaggcc
     7441 tgacattgac gaacgaagag tttgaacaac tgcaaaaagc aatagaaaat atgtaatgag
     7501 gaaggaccct gtcgaatgaa aagtatgact ggttttggca aagccatccg cgaaacaaaa
     7561 gcgtatcagt tagatattga aataaaaagt gtcaaccagc gttttctaga tgttcaaata
     7621 cgaagtccta aaatcctgaa ctttgttgaa aacgattttc gtcagcttat taaacagcag
     7681 ttaagccgcg gtcgaatcga agtatttgtg aatctagctt atattggcaa aaacgaaaaa
     7741 cagatcgtca tcaactggga attgattgat tcacttatca ctcaattgac agacggaatt
     7801 caacaacgat acggtagtca aaatcaagtg tcaattagtc ggatggcgga acgcttcgtg
     7861 ctggacgaaa acttcgtaac ggtagaagaa aaaccggaag agagcatgga agaattggca
     7921 cagttagctc tagatacatt gaaaagcgca cttgattcta ttgaggcgag tcgagaaaaa
     7981 gaaggtcaag ctttagcttt agtgttgaaa aagaatactg aagaattcaa agaagtacta
     8041 aaaaaactcg atagttttgt agaaatctat gaaaaggaat ttcaaataag gtatcaaaaa
     8101 aagctggagg aatttct
//
LOCUS       DAZUKD010000124         8073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000124 DAZUKD010000000
VERSION     DAZUKD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.127"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..2317)
                     /gene="copB"
                     /locus_tag="ACG8N9_002420"
     CDS             complement(146..2317)
                     /gene="copB"
                     /locus_tag="ACG8N9_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4897973.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHVGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2337..4523)
                     /locus_tag="ACG8N9_002421"
     CDS             complement(2337..4523)
                     /locus_tag="ACG8N9_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4897974.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4523..4732)
                     /gene="copZ"
                     /locus_tag="ACG8N9_002422"
     CDS             complement(4523..4732)
                     /gene="copZ"
                     /locus_tag="ACG8N9_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4897975.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQFEKICQAINGLGYKAEVI"
     gene            complement(4745..5185)
                     /locus_tag="ACG8N9_002423"
     CDS             complement(4745..5185)
                     /locus_tag="ACG8N9_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4897976.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5259..5798)
                     /locus_tag="ACG8N9_002424"
     CDS             complement(5259..5798)
                     /locus_tag="ACG8N9_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFS4897977.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6413..7879)
                     /locus_tag="ACG8N9_002425"
     CDS             complement(6413..7879)
                     /locus_tag="ACG8N9_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4897978.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2206 a         1762 c         1404 g         2701 t
ORIGIN      
        1 aattctgaca aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc
       61 tggaatgccg agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt
      121 tttttgtcac aacctttttt cattattatt tgattttcaa tgtcaaagca tttagagcta
      181 caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa
      241 ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc
      301 agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat
      361 cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg
      421 caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac
      481 ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat
      541 ctggtaataa accgccataa tattcttcga tccctaaata atctgctact gcttgggcag
      601 cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga
      661 taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat
      721 tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt
      781 gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt
      841 ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag
      901 gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga tgattggcat
      961 tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta
     1021 cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga
     1081 tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt
     1141 tagctgctat tgatgtggag cgagcaacaa ctaatgggat agctaaacct agagcatgag
     1201 gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga
     1261 acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg
     1321 ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt
     1381 ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc
     1441 catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg
     1501 attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg
     1561 ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg
     1621 aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg
     1681 ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta
     1741 gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg
     1801 gattgagcag atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca
     1861 tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta
     1921 aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag
     1981 aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga
     2041 ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg
     2101 aatgatccat gccgacgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt
     2161 ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat
     2221 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     2281 tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     2341 tttttcatgt gtcgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     2401 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     2461 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac
     2521 aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt
     2581 tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc
     2641 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     2701 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     2761 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     2821 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     2881 gccacggcaa tccacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     2941 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     3001 aatcgtttac gtgtacccgc aaaatagcgt actccattaa tcgtaccgct gatcccagca
     3061 cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     3121 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     3181 aggacttctt ttgaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca
     3241 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
     3301 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggggtcgct
     3361 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
     3421 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
     3481 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
     3541 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
     3601 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
     3661 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
     3721 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
     3781 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
     3841 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
     3901 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
     3961 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
     4021 ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct
     4081 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
     4141 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
     4201 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
     4261 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
     4321 taaccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
     4381 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
     4441 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
     4501 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
     4561 cttgacaaat cttttcgaat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
     4621 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
     4681 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
     4741 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
     4801 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
     4861 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
     4921 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
     4981 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacgt cccagcaaag
     5041 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
     5101 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct
     5161 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
     5221 agcttatgaa tacagattac gcttgtaaac aaaaactgtc aattattttt gctctctatt
     5281 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
     5341 cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc
     5401 cagataaaag ataaaaattc taagtattcc ctgtttagtt tctctgaaaa tgattcaggc
     5461 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
     5521 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
     5581 tctaatgtag aagtataatt tccttcaaca atccatgacg gattagatac cataaacgat
     5641 tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa
     5701 aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat
     5761 tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg
     5821 gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag
     5881 tcgtgtcaaa gtcccttgtt tcgaataaat ggtgttcaat cagctgacct tcagcattaa
     5941 ctcaaaaaac ggcaaaatat gaggagctat tttttcgttt gttcctctaa tactcaggtc
     6001 aaatcgctga ttttacaacc tcttccttaa acggtgttct cctgccaaca cctcggtcac
     6061 aaggcacaat agtcaacaaa catgagaagt tgttttttga aattgctcct tgtgactcgg
     6121 agttaaacgg caggctcact acctctttct taaacggtgt tcaaaagctt gcatctgcgg
     6181 taataagccc agcaaaacga aaaatatgag gggctatttt tgtttggctg ttcttattac
     6241 ttgatgcagg acagcttttt cacaacctct gattcacggt gttccagaag tagcttctgc
     6301 gaaaaacaag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
     6361 ttcttgaagc tgaccacttc tgtcacaacc tcttgtaaca agtatttcta ttttaaggct
     6421 gaaccactgt ttctggttca gtgatttctt tgtttttgtc tcgacgagca attgctggca
     6481 agatcatacc taataagatc aagacaattg gtgtgatgat attcgtgaat aattggaacc
     6541 accatgcttt aggatcagca gtataatcta cttttggcac cattcctagg atacatgcaa
     6601 acgcagtgaa ggcaaagcac caagcaccaa agataaaacc gatttttgga tttttaacaa
     6661 atttatattc agaattgaat ttcttataag ctttattcaa catcatatat gccaagaaca
     6721 cccataggta acgcattggc atgacaactg agttcaagtt cgtcaaccat tttactaatt
     6781 cattcatatc tttgatacca aagatcggca acaaaataat gattcctact agtatacctg
     6841 tcagaagata accattcacc aatgtacctt tacgattacg tttacgtagc cagtttggta
     6901 caaattctgg atccgcatct gctaacaaga tttttagtgg tgcatcaatt gaaaaggcta
     6961 atgcggagat ttgtccaatc atgtttgtca atgcgtaaat gatcatcaat gagttaccga
     7021 taccatagta attacctaaa atttgaaaag cactgtatgc cccatttgcc atcaagtctt
     7081 taggaatatt atcgctagca aacagcatac ccattgcgac tgagcctaat acagcacaaa
     7141 tccctaccat tgctgctaag aagatcatcc ctttaggaaa ttcctttgca ggatttcttg
     7201 tagagttgac ataaggggat attttttccg ctcctccaac tgcaaatact agcatcgaaa
     7261 cggttgtgaa ataactgaaa tcaaatgttg gaatatatgt tttcacttga ttcatatgtg
     7321 gtgtggcaaa ttccatcgtt ggattgatga acggtgccgt tacagctaaa agaataaata
     7381 agatcgacat gacgaacatc gctgttccgg ccagtccgcc aatcactttc aaagtagaaa
     7441 gtccttttgt tgataaatat aaaaaggcaa gaaatataat aagtgaaata actgctacca
     7501 tttgtactga catcgtattt acgatatttc catttccttg gactgcccaa ccgccagcaa
     7561 tcaggattgc ttgaggcttt tgtgccaaat aaggaatatg gacgacccag taagtccaag
     7621 cggcatagta tgccagtcgt ttattagatg tcgtattttc gacccatgaa ctgacgcctc
     7681 ctttactatc cttgaaggct gaccctaatt gtccaacgat caatgcgtat ggaataaaat
     7741 aaatggccaa aatcaatagc caagaggtga caactgatac tccttgctga gcatagttat
     7801 taactacatt ccccatgccc caaaccatgt taaatgcaat taacgcaaca gtaaaccagc
     7861 gtaattgttt gttgttcatt cgttctctcc tttatgataa aaatttgtta tccagatgta
     7921 atcatattca atttttaaca aacttacaag acagtgttat tttatgtgtg tattctttta
     7981 tttaatacac atcgtttttt catatttttt ccatcaaaaa aataagttat tttcttttat
     8041 ttcatttacc ttattgaatt tcaagctatt ttg
//
LOCUS       DAZUKD010000125         8033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000125 DAZUKD010000000
VERSION     DAZUKD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.128"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..583)
                     /locus_tag="ACG8N9_002426"
     CDS             complement(<1..583)
                     /locus_tag="ACG8N9_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4897979.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCD"
     gene            complement(593..1969)
                     /locus_tag="ACG8N9_002427"
     CDS             complement(593..1969)
                     /locus_tag="ACG8N9_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4897980.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LVNFINPILGLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(2089..2736)
                     /locus_tag="ACG8N9_002428"
     CDS             complement(2089..2736)
                     /locus_tag="ACG8N9_002428"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS4897981.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2820..4247)
                     /gene="uxaC"
                     /locus_tag="ACG8N9_002429"
     CDS             complement(2820..4247)
                     /gene="uxaC"
                     /locus_tag="ACG8N9_002429"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4897982.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(4321..5400)
                     /gene="uxuA"
                     /locus_tag="ACG8N9_002430"
     CDS             complement(4321..5400)
                     /gene="uxuA"
                     /locus_tag="ACG8N9_002430"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4897983.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5403..7034)
                     /locus_tag="ACG8N9_002431"
     CDS             complement(5403..7034)
                     /locus_tag="ACG8N9_002431"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4897984.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7338..8009
                     /locus_tag="ACG8N9_002432"
     CDS             7338..8009
                     /locus_tag="ACG8N9_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4897985.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2624 a         1598 c         1068 g         2743 t
ORIGIN      
        1 aatcacaacc attttcaatt acacttcgaa taatatcttc tttttttcct ttatcttgac
       61 aacacagttc atcaactaat gataataatt catgtctttc ccattcttct tgaacagctt
      121 ccttattagc tgagcttaca ataattatct tcccatattt tttcagagct tttatagtat
      181 ctctagcact taaaaagggt ttatctaatc cttttgcttg actaatctgt tgattgactt
      241 cttccgacca ttttaaagct tttaataaat cctctgactg agaatattct atttccctct
      301 tcaaagatgg attagacaat tcttttgtta gagataccca gtgctttaat cgattaatcg
      361 atgttacatc atttttttct aatgttttaa ctaaacctaa aaatctgttt attccacgag
      421 tctctgaata taaattaata ttattccaat cctgtaggaa tttttctcta tgatgaacat
      481 caaaaatctc tgcagcaatt ggtccgaaaa agagattgtg tttataagtc atcgtatcca
      541 taacacagcc atctgaatca atacaaaata tccaattatt catatttctc tcctatttat
      601 gtttttgttt taaaagctcg caattttcta agactttatc tttagataat ggataaagga
      661 aaaataaaat taatcctaca atcagaaaac aaattgatgg aattaatgtt gaagcgttat
      721 ataacttttc taaaacatct cccgtttgta catttgctaa agagtcatag cctataatag
      781 aaagagtcca acctccgacg cctccagcta gagcttgacc tatttttcta gcaaaagaat
      841 ataccgcata tacggtccca tcttctcttt gatcagactt tacttcttga tcatcaataa
      901 catcagtaat atttgcccaa ataacagtat taaacacacc caaacccata tagccaaccg
      961 aactaagaat taagaataca tacatattac tagcttttat caaaaataag atcaaatata
     1021 ctattgctga aaagaacatt cctactgatg ccagttcttt ttttccatat tttttactta
     1081 aaaacaaagt taaaggcgaa acaactatta aacccaaaat aggattgata aaattcacta
     1141 aaactattcc ctgtgcatca ttatagtaat caggaaatac ataattattc atactcataa
     1201 ttaataattg agcaaggagt aagaaaatag ccgcaccaat tatagaaagt aatgccctat
     1261 ttttaactaa cccttttaaa gattttaaca atgaaacttt gtattgagat tggtcaattc
     1321 ttactcgttc agtagttagt ttataacata ttaaataaca aataagtgca catatcgaaa
     1381 atattcccgc aacaattgta aaagttgatc caccacgaac tatttgagcg cctgaatcat
     1441 ctcttgtata aataaataat ggtgttaata caccaatgac caaacttgct aaagtagctc
     1501 caacatttcg gaatgtcgat agcattgttc tttcttctgt aatagaagta atagctgatg
     1561 ccattgatcc ataaggaata tttatagatg tgtaaaaaat actcccccat aatatatacg
     1621 taacatacat gtatactacc ttaacgctca tagaagctga aatcatagaa gtttgataca
     1681 ttaaaaaact tgctaaagcg actggaccac tcattcttaa aatccacggt ttaaacttac
     1741 cctgcttagt tgctttcgat ctatcaacaa tacgccccat agttacatca gtaactgcat
     1801 cgataaatct tgctactaaa aataatgttc caacggcagc tccgcttatc cccaatactt
     1861 ttgtataaaa gatcattaaa aaagaactag caaaaatgaa agtaaaatcg tttccaaaat
     1921 ctccaaacat atatcctatt ttgtctctta taccaaattt tctttccata caattctctc
     1981 ccttctgttg ttaagcgttt tcattctgat gaaagtacgt actatactct gaagcgtaaa
     2041 gagtcagtat ttatttttta catttcagag tattatttgt tttttctcct aataattctt
     2101 tatctcataa aacttatcta tatatttact agctactcga cttacttctt caaaatcacc
     2161 tttttcaaca gaagccaata aattcccacc tacaccaact gcaacaactc ctacttcaaa
     2221 ccaatcttct aagttctcta tacttactcc gccagtaggc attatattta cgtgtggtaa
     2281 tggagcttga taggctttta caatggatgg accaaaagca gaacctggga atagtttaat
     2341 gatatctacg ccagcagtaa gtgcctcctt tatctcagta attgtcatac aacctggtaa
     2401 ataaggaatt tgatataaat tacaaatctc agctgtttgt cgatcaaaag atggactaac
     2461 gatatattct gccccagcca taattgcgat acgagcagta aacgcatcta aaaccgtacc
     2521 ggctccgatt attacatctt tttgatcagc atatttttta gcaatttctt tgattatttc
     2581 ttcagcatta ggaactgtaa aagttagttc aatcccttta attccacccg caataattga
     2641 atttattgct ttaatggcct catccttatt tttcccacgt acaaccgcaa tgactccagc
     2701 cgcttctaac ttacttaaaa tttctacttt tttcatatct atctccttcc tttatggata
     2761 catactttgt ctagctgtac taacttacaa agaataaatt taagctttta tttcaagtat
     2821 tattttctac ttccgatttc aaaaaaatta aaataattat acgcattacg ataacaaata
     2881 ttctccacta tctgactaag atacatttca tcatctggaa ttcggcctgc tttaacccac
     2941 tcgcttagta attgacataa tactcgacga aaatactcat ggcgaggata gcttagaaaa
     3001 cttcgagaat cagtcaacat acctgtaaaa ttcgacaaca aactttgttg tgccatcgta
     3061 gtcaactgtt gacgcattcc ttcagctgta tcattgaacc accaagcaca tcccaattgg
     3121 attttttgaa tcataccccc ttggaagctt tgcattgctg ttgctagtgg aagccaatca
     3181 gttggattta atgaatataa aattgttttt ggtaagcacc cactcttttc agcggtatcg
     3241 aacaatgcta caacattttg tgtcaaacta gcttgatccc ccatcgaatc accaccgaca
     3301 tctactccaa tttgttcctt taatatactt gaattatttc ttaaagcatt taaatgcatt
     3361 tgcatcgtcc aaccatatct tgtatactca gacatcaaat gtaattgaat acctgtaaca
     3421 aattgtgcaa tttctgataa atggaaatcc gtttttcctg ctaaagcttt tattaacaga
     3481 ttgtcgagta ttttttcatc tgctatttca taaaaataag tattcaaacc gtgatctgct
     3541 aatcgacagc caatttgatg aaaatgatcc actcgttggc tcaatgcttg cttcaaccca
     3601 ttaaagtctt tgattggtat agccgctgtt tgactaagtt ttaaaatata gttcgaaaag
     3661 cctgatgatt tgatactcca gaccttatct ggacggaaag taggtaatac cttgaaagaa
     3721 ttatcctgtt tagaaagctc agtatgatac cacaagtcat ctgtaggatc atccgttgta
     3781 cagatggctt tcacatccat catctccaac aattctttcg ggcgatagga atccgtttga
     3841 attttttcgt tagcacgtcg ccaaatttct ggtgcattct tctcagaaat tgtcaaatca
     3901 atatcaaagt aacgttgtaa ttccaaatga gaccattcaa aaataggatt acctggcgcc
     3961 ttctccactg tttttacaaa agctaaaaat ttttcataat catcacctgg accactgatc
     4021 aactcttctt ctataccatt agctcgcatc aatcgccatt tataatgatc tccagctcct
     4081 ttatcattta accatacctg agtcaaattc ttgtattgct tattttctag caaatatgct
     4141 gggtctaaat gacaatgata gtcgataatt ggttggtgtt ttgcaaattt atgaaaaagt
     4201 cttttagcta tttcattctc taataaaaag ttcttatcta aaaacatatt taaacactcc
     4261 tttccttccg ttagttttta gaagagaaaa tttttcctct aaataaactc ccttatataa
     4321 ttatttactc gccccctttt caagcgcttc ccaaatacca tttaaataaa ctgcacctag
     4381 agctcggtca tataatccat aaccaggtct accggtttct ccccaaatca tccgaccatg
     4441 atctggacga atatagccat taaatccatt atcgtacatt gtttttaata tcttatacat
     4501 atctagagaa ccatattctg atttatgcgg cgcttcgtaa aaatcgagtc cattgtcact
     4561 taaatatttg atattacgaa catgtgcaaa aggaatccga tcccgttgac aaaattcaga
     4621 tagaatttca tagacatcat tatctggtcg ttctgcaata cttccagtac ataatgtaat
     4681 accattagaa ggagaatcca ctacattaca caaccaatct aaatcgtcac gatttttaac
     4741 aatacgaggt aatccgaaaa tggaatatgg tggatcatct gggtgaactg ccattttaat
     4801 cccaacttct tcacatgtcg ggataattgc tttgagaaaa tatgcaaagt tctctcgtaa
     4861 tttctcttca tcaacttctt tataggcttc taataattgc ttaatttgtg ataaccgatg
     4921 aggttcccaa cccggcaatt caaaattatt agtagaattt tctacttctc taataatttc
     4981 ctccggatca gtagggatat tttttgaaat ataggctaac gtagaagacc catcatctaa
     5041 acgataatcc aaatccgatt ttacccaatc aaatactggc ataaaattat aacagataac
     5101 ctcaatccca aattctgcca aattgcgaat cgtctgttta taattctcaa tgtattgatc
     5161 tcgtgttggt aaaccaattt tgatatcatc atgaacatta acactctcaa taactttcaa
     5221 ggtaagtcca ttggcttcga cttcatcttt taaagattta attcgttctc tcggccaaac
     5281 ctctccggct ggtatatcca ttaatgttcc aacaattcct ttcattgctg ggatctgacg
     5341 aatttcttgt agtgagattt tttcttcatt tgccccatac catctaaaag tcatttccat
     5401 agttactttc ctccaacttc tgttatatat ttattgattg ttttacgaac tgcttgagga
     5461 cctactagca tttcgcaaaa gattttttca gttttctttc ctaccccaac ttgatataaa
     5521 tctaaaccaa agatttcttt attttctaaa atttcctgta ccgcacaatg aatctttgaa
     5581 ggatcttgtt cacctaattt caatactgat aatttatcct gcaactctac caataacgga
     5641 tcaggacttg gcacaaaatt ttccccttga tcatcgactg ccagtaaata acgtaaccaa
     5701 gcagcaatag cgataggaat aaatgttaga ttttctgcat ttccgtcttt ttcgacatac
     5761 tttttaattg tttcaccata gcgaatcgct actttttgag aagtatctgt tgcaattcgt
     5821 tgtggtgtat ctggaatata gggattaggc aaacgtttat taattacttc atcaataaat
     5881 tgttttggat caataatgcc tggattttca actactggta aaccttctaa ataacctatt
     5941 cctttgatta aaccaaccaa atcatcatcc tgcatctctg ctgaaattga ttgatatcct
     6001 agcagacaac ctgttactgc caaagcagta tgaagtggat tcaagcttgt cgtaaccttc
     6061 atgatgtctg ctttatcaac cgtctctcta tctgtcataa tcacaccagc cttttccaat
     6121 ttaggccggc catttggaaa actatcttca ataactaaat aatgaactac ctcagtatta
     6181 gtgaaagcag caatattggt atgttttttt gtgtgaatga tatctaaatc ctcgagcccc
     6241 atcatcaata gatcttcctt tacattctct gaaggatttg gcgtaattcg atcaatcata
     6301 ctccaaggaa aagtcacttt ttctttatcc tttaaataag caataaattt ttcagaaaca
     6361 tatccttctt tttgccaagc ttctgcaatc gtaaaaatac tatcttgaaa acgttgacca
     6421 ttctgtgaga aattatcagt agaaaccatc gcaatcggca actctccagc attaaatctg
     6481 ctatataaca aggatgttac aatactcatc gtatgttgag gattgctagg tccagatttg
     6541 atatcagctt tgactaccga aaagaattgc cctttagaat cttttaaact gtaacctttt
     6601 tcagtaatag taaacgttgt aaactgtaat ttaggatctt caaaatattt aaccactctt
     6661 tgatagtccg ctatattctt cggattgcaa tataaactat cagccgttac tgccaaaagt
     6721 ctttttttga gttcaccatc tccactcata acaatttcca aaatgtcatt gttatagtca
     6781 tgataagctt tttccactac ttcttcatca aatgtttcac acacaactac tcctgtttct
     6841 aattcatttt gatctgctaa gcattgagca atttctgcat gaaatcctcg ataaagattt
     6901 cctccaccaa aatgaagcca tactggatgt tgctggcctt cttgcttaac ttctttgaca
     6961 tcaaaacacg gcagtagaat ctgcatttca ttgaattctt cgatagaatt ttgccaattt
     7021 ttaatcgaaa ccataattaa cctcctcaaa ctgatatgtt agttttgata cattttatat
     7081 tttaaaaccc attgtaaccg ttgtcaatat aaaaagggaa taaacttgct tttttctacc
     7141 atatcagaat gattgtattt tttattcaga aaaataaaat actccttctt tttggactga
     7201 ttttatcgaa acttattttt tctttttttc atgtattctt ttatttttat aactagtgta
     7261 ttacttcaaa atttgatatt atatggcatg aacagaatta aaatatcagt taaaataaaa
     7321 gttaaggagc attgtgtatg acaattaaaa ctagtctcca aaatcaagct taccaatcta
     7381 ttcgccgaca aattatctat gctgacttag aacctggaaa aaaagtatca gaaaaaaaat
     7441 tagaagaaac actttctatt ggtcgtacac caatacgtga agcactgatt caactccgac
     7501 aacaaggtct tgttgataca attcctcaaa gcggaaatta tatctcatta attgatttag
     7561 attcggccaa aaatgctcga tttgtcagag aacatttgga acgacaaatt atgttagagt
     7621 gttgtgccaa aatgaataac caaaaaaaac ggattttaga aactataatt tcagaacaag
     7681 aaaaagctgt tagtcaaaaa aatgctcgta gtttttttca taatgataat ttattccata
     7741 aagcttgttt tgaaattgca gagcgagaag aagtatggca atggttagaa aataacaata
     7801 cacacttaga acgatttcga tggctacgag taaaaacgga aggattaaag tgggaaaaaa
     7861 ttatggaaca acattatcaa ctatttgagg cattaacaaa acaagatcca gaagaagcta
     7921 actttctaac atcgttgcat cttcatttaa tgcttaatga gcaaaatatt gttttagcta
     7981 ctcatccaaa atatttcaaa aatatataac ttttaactct ttttaaatta aat
//
LOCUS       DAZUKD010000126         8006 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000126 DAZUKD010000000
VERSION     DAZUKD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.129"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..1074)
                     /locus_tag="ACG8N9_002433"
     CDS             complement(325..1074)
                     /locus_tag="ACG8N9_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4897986.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1260..1964
                     /locus_tag="ACG8N9_002434"
     CDS             1260..1964
                     /locus_tag="ACG8N9_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4897987.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2004..2561
                     /gene="hxlB"
                     /locus_tag="ACG8N9_002435"
     CDS             2004..2561
                     /gene="hxlB"
                     /locus_tag="ACG8N9_002435"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFS4897988.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2569..2877
                     /locus_tag="ACG8N9_002436"
     CDS             2569..2877
                     /locus_tag="ACG8N9_002436"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897989.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     "
     gene            2935..3708
                     /locus_tag="ACG8N9_002437"
     CDS             2935..3708
                     /locus_tag="ACG8N9_002437"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897990.1"
                     /translation="MINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQSLGKVL
                     GGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAAHAAVAY
                     LGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKTLNRHGN
                     QKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYRNLADPE
                     AEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3720..4151
                     /locus_tag="ACG8N9_002438"
     CDS             3720..4151
                     /locus_tag="ACG8N9_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4897991.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4165..5610
                     /locus_tag="ACG8N9_002439"
     CDS             4165..5610
                     /locus_tag="ACG8N9_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFS4897992.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            5896..7521
                     /locus_tag="ACG8N9_002440"
     CDS             5896..7521
                     /locus_tag="ACG8N9_002440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4897993.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         2680 a         1164 c         1619 g         2543 t
ORIGIN      
        1 tttgtattat acgaatcaaa ttcctcaatc gtcagtgaac ttgctaattt aatgatagca
       61 ttgctgttta ctcccgcgtt gagagtcata actattttgg tctgtttagt gctattgttt
      121 tttgttaata aaaagaaata acgtagtatt tcaaaagaca aagaagtggg atgatgattg
      181 ctatagtatg ttatacgtgg tgctaggtaa gtgagaataa acaaaaaagg aaacacgcaa
      241 taaagtttat ttcaaaactt tattgcgtgt ttctttttta gattttttat atctgttgct
      301 ttagtagtta ccaatagttt tcgcttaatc aatcaaaacg attttttgtt tattgtattc
      361 tttacgaacc ttttcaggaa tttcttctga aataagatag gtaaggtctt caattggacc
      421 gacgatatga ttactgatgg tgccgaattt atcattagtt atcatcccaa gtacttcagt
      481 agaaatacgt atcatctcac gtttaatgtc ggattcttgc atagttggca cgctcgttcc
      541 aacatctaga tctatgttat aaatacccat aatataaagg tcggctcgaa tgttttgtag
      601 ttgcttataa gcttgtaccc caatattgat catagattgt ttataaaaag taccgccaat
      661 attgataatt tccaaattag gatgctgtgc taatgctatg gcaatcggcg ggctattggt
      721 aatgattgtt gcacgaaaat cttgtgggat ttcttttgct aaagccaagt tagttgtact
      781 tccatcaatg atgatgacag attcttcttt taaaaaaggg agggctttat gagctaatgc
      841 agttttttgt tcgctagcta cagtttctcg gtagttaaaa ttggtaattg gcggaccaac
      901 acgtaaagcg ccgctgtgta ctctttttaa gagaccttgg ctatccaaat ctttcaaatc
      961 tcgtcgaatc gtgtcttcgg atacattgaa acgttgactt aaatcactag cgataacttt
     1021 ttgttctgcg tttaataaat ctagaatttt ttgatgacgt tctaatttca acatgttgcg
     1081 tgctccttct tatttattct tgtttatcat gtcatttatg cgtttttatg tcaataattt
     1141 ccgtttttaa tacactaaaa aggcataaac aagcaaaaac aaaatatttt tatgcagttt
     1201 tatgttgact agtatttgtt aagcggttac aatgaggggg taattaaagg agggaatgta
     1261 tggagaaaaa aattattttg tcgccttcac ttatgtgtgg ggatctagca aatttggaag
     1321 gaagcatcca agaactagaa agtattggga tggattcctt gcatattgac gtgattgacg
     1381 gttcttttag tcccagtatg cctttaggga tcgaaacaat caaaagaatt agagaaatca
     1441 cttcaatgaa ttttgatgta cacatcatgg ctaaggataa cgataacgaa ttttttattg
     1501 gagaaatgtt gaaaattggc gtagagagca ttacttttca ttatgaaaca agcctccata
     1561 ttgatcgata tatccagctc atcaagaatt ctggaacaaa agtagggata gctttgaatc
     1621 cagcaactcc gttaaatgta ttacaatata ttttacccga gattgatagg atttgtttga
     1681 tgctgattaa tccaggtttt gcaacaaaca agtcagaaaa acaagtttct tacgcgctaa
     1741 aaaaaattac agatctttgc cagttagtaa atgagaaaga gttagcagta gatatccaag
     1801 tggatggacg ggtatcgtta gaaacgatcc cgggattatt agatgcaggc gcaacaaatc
     1861 tggtattagg aagtacaagt ttgtttctta aaggaaatac attagcagaa aatcaaaaac
     1921 tactttatca agcgatgaag caatcagtta ggaaaaatca atagatgtag tggtcctgaa
     1981 taatagaaat ggaggggaaa tagttgagtt ataaggcggc gaaacaaaat attgtaagag
     2041 aaattactga aacattagac cgtatttctt caaaagatgt ggaacaattg ataaagaaaa
     2101 ttgaaaaagc ggaacgagtt tttttcgtgg gggttggcag agtactgtta gcgctagaag
     2161 gaatggcaaa acgcttaggg catctaggaa ttcagacagt agtcgtagga caaatcactg
     2221 aaccggcaat tacagataaa gatttattga ttgttggttc tggtagtggg gaaagtgctt
     2281 tcccactaac tattgcaaaa aaagcaaagg aatttgggtc ccaagttgcc catataggat
     2341 caaacccaga cagcacaatg aaagaatatt cagatctttt tattcgtata cccgtacaaa
     2401 caaaattagc gttaccagag gaaatttctt ctatacagcc aatgactagc ctgtttgaac
     2461 aaagtgttct tttgttagga gatagtattg ctttagtgat gatagaagaa aaagcaattg
     2521 atatgtcaaa gttgtggcaa taccatgcta acttagagta ggtgaaaaat gaaagcggta
     2581 cattttggag cagggaacat cggcagaggg cttattggac aaattctaca tcagaatgga
     2641 ttcgatctat gttttattga tacgaatcct gaactgatcc atcaattaaa tcaagataag
     2701 ggttatacta tcgaaattat tgatgaaatg aacaggagtg atttcattga tcatgttgtg
     2761 gcactgaata gtttatccga gctagaagag gtgattacag caacaaaaaa tgcagatatc
     2821 ttaacaacat ctgttggcgc aataaattta gtaaaaattg caccgatcat tgcgtaagcc
     2881 ttaagtaagc gatttgaaca aaagcatagc atcaatatcg tagctaatga aaatgtgata
     2941 aatgcaagtg atgtgttgaa aaaagaaatt tattctttgc tgtcagaaaa agaacaagag
     3001 aattttgatg aatatgcata ttttgtgaat acggcaatcg atcgacaatc tttaggaaaa
     3061 gttcttggag gaaaaatgat ctctgttgtc gaaccttatt atgaatggat aatggatcga
     3121 cagcagttag atccaactac atcttttgat atgaaaaatg taaatttggt tgatgatatg
     3181 gagccttaca ttgagcgaaa gctttatatc gttaatgctg ctcatgcggc agtagcttat
     3241 ttaggcgcac tgaaaggata taagacgatt caagaagcaa tccaaaatat ggagattttc
     3301 aatctagtca agcaattttt aatggagaat atagagtact ttgtacaaaa atatcatttt
     3361 tctagagaag aattaacatc ttttgtggaa aaaacattaa accgtcatgg gaatcagaaa
     3421 ctctccgatg aaattactcg tgtaggaagt tcccctattc gaaaattagg ccctaatgat
     3481 cgtttaattg ctcccatagt aaaactagat tcatatcatt taccttacga tgcaggaata
     3541 aaagtaattg ctgcgggata tcattatcgg aatcttgctg atccagaagc tgaacaacta
     3601 cgtcgaatga ttgtaaatga tgggttgaaa gcaacgatta aaaagatttc tcgtttggaa
     3661 ggtaagctgc tgaatgaagt tgtcgctgct tatgagtccc agatttagag gaggagaata
     3721 tgctaaaaga acaattgtca ggaaatacaa tgtttatgga tgcgatcgat tcatggcaag
     3781 aggcaatccg agtaagtgcg cgtcctttga taggaaaggg aataatagag gaaagctatg
     3841 tacaagcgat gatacaaaat gtgttggata acggaaatta tattatatta ctgccactag
     3901 tagcaatgcc tcatgcgcgt ccagaatttg ggagcaaagg ggtcgggatg agctttttat
     3961 gtttagataa gccagtcatg ttcccaagtg aagaaccagt aaaattcttc tttacctttt
     4021 cttctgattc gccaaaggga catttagact taatggccaa tcttggagaa ttgctgagtg
     4081 atgaaaaaat gtatcaaaaa ttattcgatg tgaagacaga aaaagcattg ttagatttga
     4141 taaagaagta aaggagagaa gaaaatggag gtagcaaaca aaaaaaaacg agtctccatg
     4201 cgagctaaag ttcaagcgat tggcggtttc ttaacaaata tggtattgcc aaatataggc
     4261 gcatttattg catggggaat tgttactgca ttattcattc caactgggtg gttcccaaat
     4321 gaatctctga atgaactcgt aagtccgaca gtcagctatc tattgccgtt actacttgcc
     4381 tatactggtg gtcggatggt tggtggtaca cgtggagctg ttttaggtac aatcggaaca
     4441 ataggtttga ttattggtgc tgacatacca atgttcatgg gagcaatgat tatggggcca
     4501 cttggtgggt ggatgatgaa aaaaacagat gattttttag atgggaaagt tcctgctggt
     4561 tttgaaatgg tcgtcaataa tttttctata ggaattattg gtttcatact gatgtgcctt
     4621 tcttatctgg tagttggacc aataatccaa tcagctaatc aatttgtaac gagtgcaatt
     4681 gaagcattag tttcaactgg ttttttaccg atactgtcct taatcaacga accggcaaaa
     4741 gtcttatttt taaataatgt tattgaccaa ggtgtttatt atccattagg tatgcaacaa
     4801 acattagagg caggcagatc aatttatttc atggtagcat caaatcctgg ccctggctta
     4861 ggactattgt tagcttattc tttatttggt aaaaaggcag caaaacagac agcaccaggt
     4921 gcaatcatca tccatttttt cggcggaata catgagattt attttccata tgtattaatg
     4981 aaaccactca ctatattatc tatgattgct ggtggaatga caggaatcat cacttttgaa
     5041 ttatttgatg cgggcctggt agcaggtcca agtccaggtt ctattttttc ttatttagct
     5101 ctaacgcctc ggggaaattt tttgggtgtc atggcgggag tggtcacagc aacactggtt
     5161 tccttttttg taacatcagt aattttaaaa gcagataaat caacagatga agacagaaat
     5221 tttgaactat cagttcaaaa atcgaaaacg atgaaatcag aaggaagaaa attattaaaa
     5281 gaagaaaatg ctagagaaat atgtaccgag aaaaaagaga tgaataaaat ttcctttgct
     5341 tgtgatgcag gaatgggaag cagcgcaatg ggcgcaacaa tattcaagaa aaaattgcaa
     5401 aaagcaggta ttactgatgt tgagataaaa aattatcgaa ttgaagaggt accagaagat
     5461 agtgatttgg tgatcgtaca tcaagatttg gaggaacgga ctagaaaagc acatcctaac
     5521 atgaaaattg tgacattaaa gaattatttg caagatccag taatcaatgg attagcagaa
     5581 gaaatcattg gattaaaaac agaagactaa tttatgggaa tttttgatta acctttatta
     5641 aaattatttt gcctaatata aaaaggttgt gacatggctt gtgtcgcaac ctttttttgt
     5701 cgcacaaata attaatcgga tattttatgt tcatattttt tgtgatcaac gagaactgat
     5761 ggggcaaaga tacattttag atacttttaa ctgttaattt acaaaacaag gtgaaacgaa
     5821 tagaaaaatg aaaaaatcga tacggaagaa tatgtttgat taaaacatgc atgaagagaa
     5881 ggaggttgat gatacatgaa aaaaatatat tcaaaggaaa tcgattgttt tagaggcatt
     5941 gctgttttag cagtgatatt ttatcatttg ccaaatagtt tgctgccagg cggattgcaa
     6001 ggagtaacga tcttttttgt ggtttctggt tttttaatgg ctaaaaatac gataagagat
     6061 gaaaaaaaca gacaattcag ttttttacat ttttactata agcgtatcaa acgtatttat
     6121 ccatttttac tgttttcttt ggtcgtattc attttcatac tagggattag caacattcga
     6181 ttgataggaa acattcgtag tgagctaccc agtttgattt ttggctataa caattggtgg
     6241 cagctgaatc aaggagtatc ttattttgac tcttacttta atgcttcttt atttaaacat
     6301 tattggtcgc tttcagttga actacaattc tatttgatat ggccattttt gtttattgtc
     6361 attaagcgta tgagaagaaa acaagtgttt tacttaatct atacattgat ttttgtttct
     6421 attcttttta gtttgttttt accttcaact aaggcatatt atcatacgat agctaaactt
     6481 tttccatttt tattaggagt ttgggggtat tttaatcgaa taactattgg ccgtttcttt
     6541 gaacaaaaca cttttagtaa aatctggctt ttattactag ctagtctttg tctgattctg
     6601 ttcccgattt ttccttatac actaaatgaa ctactgatca gtatttgttt tgctctattg
     6661 ttagccagtg tcgatgatat gaacattgca aaaaagacca aaatcgctag tacaaaactt
     6721 tcttttgtag gaaaaatcag ttatgaactt tatttggtgc atttcccagt attgattgct
     6781 ctgacaaatc tattcaaaca tcaccattgg aaatatttgg atatcgcagg tctattcact
     6841 gtgtttttat gtacattact catgattact tgctttagaa aaggagtgca aaagatgaga
     6901 tcacccaaaa tttggacagc tatggcacta atgataatcg gagtttctgt actggtatta
     6961 cgtgctcctg ccagtcgact gaccgctgac caaaatcagt tgcaaagttt attaaaagaa
     7021 aactctagta aagcaaatgc aggagaaaat ctgttgtcac aagagaagaa aatattattt
     7081 atcggtgatt ctgttatgct gggggcgtat caggaactaa aggagacatt taaaaaagaa
     7141 gcattggtag atgcaaaaga atcaagacaa atcactgcgt taccagaaat cttaaaggag
     7201 cataaagatt tagtttctta ccaacaaata gtgatcgggt taggaaccaa tggtgtgata
     7261 acagatgaag ctatagaaca aacaatgtct ttgctgaaag ataaaaaagt atactgggta
     7321 aatataaaag caccaactgg ctggcaagat acgataaatg ccacattagc tacattgcca
     7381 aatcagtatt ctaatataac gatgatcgat tggtatagtg agagcaaaaa tcatcctgaa
     7441 tttttttatg atgatgaaac ccatttgaat gaaagtggac gtacagctta tgcaaagtat
     7501 attgcttcag ttattcaata aggtagtgat tacgtgtgtt aaccttactt tttcctaata
     7561 ggtgaatcaa tttaaataaa aaatacttca tatgataaac ggtgtttaag aggaggagct
     7621 ttttaatatt cgtggtaaaa aagagatggg cgaggtcgtt aactgagagc ttcttggaaa
     7681 gaaatcgctt tggatttatc ctctttgtta tacagaacat gagcgagacg acaatactag
     7741 gatgtatgtt ctgatcgaag ttttatggtt acaagcagca atcagcttag ataaaattaa
     7801 ggatcgtagg aaccaagtaa agaaggctgt tagtaaagaa acaaacgatc aagtatgttt
     7861 tactcttaag aagatgcagt gaataaataa tagtaatata atgctagaag ggactgtgac
     7921 aaaagacgtg tcacagcctt ttttgttatt ttaattatcg tccagtcttt ttttataaat
     7981 gattgcttaa gcattaaata ttaaaa
//
LOCUS       DAZUKD010000127         7842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000127 DAZUKD010000000
VERSION     DAZUKD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.130"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..1454)
                     /locus_tag="ACG8N9_002441"
     CDS             complement(90..1454)
                     /locus_tag="ACG8N9_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4897994.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1451..3010)
                     /locus_tag="ACG8N9_002442"
     CDS             complement(1451..3010)
                     /locus_tag="ACG8N9_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4897995.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3066..5546)
                     /locus_tag="ACG8N9_002443"
     CDS             complement(3066..5546)
                     /locus_tag="ACG8N9_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897996.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5530..5865)
                     /locus_tag="ACG8N9_002444"
     CDS             complement(5530..5865)
                     /locus_tag="ACG8N9_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4897997.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5879..6913)
                     /locus_tag="ACG8N9_002445"
     CDS             complement(5879..6913)
                     /locus_tag="ACG8N9_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4897998.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7003..7683)
                     /locus_tag="ACG8N9_002446"
     CDS             complement(7003..7683)
                     /locus_tag="ACG8N9_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4897999.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2245 a         1567 c         1424 g         2606 t
ORIGIN      
        1 attcttccgc tatttcatta tatgaatttt ttattctcga ttgattaata tttattatat
       61 ccccacacaa taaaagaccg tgatatttct cacagtcttt tatctactct attgctattt
      121 gctctcttag ctagtacttt aaaagctaac tcattgaggt agtgaaaacg agaggtgtat
      181 tcagacagaa atccaaaatc ctcaactgct agctgtggaa tcgtagtttt gtgtttcacg
      241 atatccgcaa gcaggttcaa atctgataat aagaattctt ggcttatcag ctgtccgcca
      301 attatttcta atgtatccgg atggtagatc agctttgcat ttactgtttt gttttcttcg
      361 tcgtattgtg gaaatagatc aaattctcct gaacacgaac cggtattttg ttccaagaat
      421 ggcgcttcat cttctgtcag ccctacagtt cctaaaaagt agtctataat gactgctgct
      481 gttatctttt gtctgaggtc gtaagcggtt ggctgaccac tcaaagttag cgctactgct
      541 cttgccatat gtacagctcg agtaactaaa ggtaaaaaga gatgccgttt atttttgtca
      601 aaactgactg ggacgatatc tccaatcgca tagatgtccg gatcacttgt ctgcagatat
      661 tcattgacta tcacagtctc atcctcatac aaagccactt tttcttttaa caatgtcgta
      721 ttaggacgag tttgggcaga aaaaatgacc atatctgcct ttattgtttg agacagaagt
      781 tgtacggata ctactttctc ttctgatttt ttccaatcga tcaagaattc atttggataa
      841 aaatttattt gcttagtttg cagctgattt tgaaaactgt tgatcatatc cttatcgaaa
      901 tatcttgata aaacattcgg catataatca actagatgaa taatgtatcc tttatttctc
      961 aaagcatctg ctaattccaa tccaatgtaa ccagcaccga agatcactac ttcttttgct
     1021 tcttttaaag cggcaagtac ttgcttagat tgcactagat ttttataggt gtagacattt
     1081 tcagcttcaa tttgtttata caaggtgaca ggagaggaac ctgtcgctac gattaattta
     1141 tcataggtta tggtctcgtt ttttccttct ttggatgcga ggatgagctg atgtttgtct
     1201 ggatcaagtt cagttactct ccattcggtg atcagttttg ccccactttc ttgaagagtc
     1261 cgcatgttat gggcaacttc agctaaacta cgtatctttc cttttaaata aaggttgatt
     1321 ccatttgaaa catagcctag tccatcttta tgatcttcat cgatcagtaa aacttctgct
     1381 ttcggttgaa gttttcttag atttagggca gctgtgatgc cttcatggga agcaccaatg
     1441 atcacgattt tcatgtttta ttcatcctta aattcaattt ttctaaataa cattctttta
     1501 aaaattggtt caaatagggg aaatcataat tatattcgtt gctagtgaat acaaaaactt
     1561 tagcggactt ctgtgacggg tactctttca attggctagt caaaatgata tcatactgct
     1621 tttctttctc gtattcgact aaatcatatt tgatcgtatt cagcaattgt tcctggatga
     1681 tttctttcac gatatccgcc attagccttt cataaggaag atcataagca atttgcagac
     1741 gtaattctaa ctgcttgatc agcgtccgta aaatagatgc atagcgagca aataattcaa
     1801 agcgggcatt ttcctcgtct acttcagtaa aatcaacaat agcaaatgtt tgtctgacca
     1861 atctttcaca taaaaccagg aattgtttat cttctacttc agttaaccgc tgttttaatc
     1921 cttcttggcc aaaaatattc atccatcctc taaaataggc aaaatgagcg tgattttgca
     1981 tcactgtcaa cgcaatcttt tgcgaaatct cttctggaaa aacataaaaa gagaaatatt
     2041 cttctatcga ttgtttaaac gttcgattca attgagctac tacgtcgtct ttgtattttt
     2101 cgcttatcaa aaactcctgg acatgcgagt tatgtgtatc taatgtaaag ttactgatca
     2161 tgaaaagata aaaaaccaac gtttcatttt gattccaagg aaaggcatat tgaaagaagt
     2221 aacgggtcaa aaatgtatgg aggtccaata aaaattgtgt cgtttgaaac aatccaacag
     2281 gaagattgct ataatctggt ttggattttt tcgtacagcg cattcgcttc ttcataatgg
     2341 aaagccatat ttcaaatttc aatcgtcctt cttctgtaat agcaaaacta aactcttttt
     2401 gcagcagatc taaaaaagtg tttcctgata aatcactgta ttcttttttg ttttcaagat
     2461 agggacgtcc aaaccaaaat aattggaaat aaaaataacg aatttgactt tctggaccgg
     2521 tcagccgccc gctttttatt ttcagatgaa actcatttaa agaatcattt aattccttca
     2581 ctctgcgaga caaagtagcc gaactgatat taagttccat gacaagtttc tgccgatcaa
     2641 atgagccatt ttccaacaga taaagaagta tctgatattt tgtagaatag cgcaagtaac
     2701 aatgataata aacatcgact gctgctatat ttccatgtct gattaataaa atattccctt
     2761 cttggataac aatttccaat tgtttgatat tttccaagtt agcttgtaaa taaaacagat
     2821 cttctttcaa ggtagcccga tcaactgata agcgggtaag tacgttatta acggaaacct
     2881 ctcctttttc ctctaataaa agttggaaaa gtttgatttg ccgttgttca tttttttcaa
     2941 gcaaactgaa aagttccaag tccactcctt caagcatttc tgattttaac agcagaagtt
     3001 tccttctcat ccttgaaaaa gaggatctgt gacagttatc acagatccat acaaattcta
     3061 atcacttagc tggttggccc agctgtcagt tcccatgtga ccatgccttg atacgttgcg
     3121 ccaggttgag cattcgtact gctatttacc tctaagagta aaccatcggt atcgggaaag
     3181 tcaacgagaa cctctccttt agctgttcca tcactctcat aaatcaacgt gttttgacct
     3241 tgctgaatca attgctttga tccagaatga ttgaagtata attttgttcc gcttaattct
     3301 tgaccttgat cattcttaaa taaagaagtc aatgtacctg tcagttgcca aggagacgtg
     3361 cttggaagcc gagtatcccg cacatcaaaa gaaaaatcgc cttttgattc ataataactt
     3421 gtcttcaacg gtttggggag atcgttacca aatgtaaaat catctggtac attaactaat
     3481 gttaattctt gtttggggac aagaattgtt ttagcagatg tttggacaac tgggttagga
     3541 agcttagcac catctgctaa tgtaatattg ctggaaagta cagctgcctt tgtcagtatg
     3601 ctgacggatt gtaaagaagt atctacagta tttaattgat agttgtaagt aagatgaatg
     3661 gtatctccta ttttgctcaa tagatctgct gggacagaaa ctccttgttg aatgccggta
     3721 tctgcgatag ttgtaccatt taattttaag gaaccattga tataagtagt attttcagga
     3781 agtgagatcg tgattttttt gtcggttatc gcatccccag attttgttaa cgtgaatgtc
     3841 acattttctt gaatcgtatc tcctgataga gcagacaggg gatctcctgt ctcaggttgt
     3901 ctgctaaggt tttttagctg actatctgca ctcacagatg gaaaattagc cactgttatt
     3961 tgaggaagag tggtgattcc agcttgttca gactgccatt tgtacgcaaa agacggatac
     4021 atgttagtaa gtacatcgtc caccaagcga taatcaaaat aatattgccc tgaagccaat
     4081 gcaggtagtg tagaagtagc tgtatttaca cccccgttat ttgattggat cgtttggaaa
     4141 agttgatctg tccatcctgt cgctaatttt tgggttcctt tcgaataaag cctgtaatgg
     4201 atcgaaccct tttttagttc agttgctgac caattggccg tgacttggta cgatgcagaa
     4261 gaaaggagct ttccccactg actatcggct gttaagtttg ttggtaaaac cgttccgtct
     4321 agcttgtact gctccaatac ctttaaagca acaggagaaa gcaatccgcc ttgcgcatct
     4381 ttcccactgt acacgatatt ggctgtctga ttcaatccac ttgtgcttgc tggagtatga
     4441 gtcagtattg ctccttgtgt aacgtttttt cctgtaaaat agttgactac gcgatcaaag
     4501 cgactaaatg gcaactgatc aaaacgtcct tcagtcagcc ccatcgtcaa tgtccgacta
     4561 ttggtagagc ctggaaggtt ctggttattt ccatccacgt attctgtggt atcagcaaat
     4621 gtattatatt ttcctgttga atcacttggt gagatgtaac tattccacgt atttatctct
     4681 gcacttgcag aaaatgcata tgctcctgga ttcaagtttc cgccagttaa tttaggataa
     4741 aatctacctg cagctggagc taaaatcgaa ttgaacatac gatcttggat aatattattc
     4801 gaatcataaa aatcaacaga aaaaacggcc tttttagaca tagaaggttg taatttaccc
     4861 ttggtcaaaa ttttgtttgg actcgtatca tatttatctc cgttttctgt catcttcgcg
     4921 acattagctg taacatcaaa ttgaagcggg atatcgtcca cgttgaattc gcctcgcgta
     4981 ataatagcaa taatatctgt caatagagta tttgtatccc acgaccccat cccaaaacgc
     5041 aaggtatggc ttcctgctga taataaactt ccgctagtaa ccttgatggg ggcaccggca
     5101 atatacaagt aggcgttgtt ccagtcaatt gcttttaata catcactgct tgatacatgt
     5161 tccggcaaat tgatgatcgc cacaaatgca aatccttgtg cagtgatatt caccaaatca
     5221 agtacatttg ttagtttcac atggctttcc caatgaaaac ggaccgtgcc acctttccaa
     5281 ggatcatttg aaccatcgtt caaataagta gcagtcaatt taggcaattg gttagcccca
     5341 gttagttgga caagcttccc gccattgact gcactacgtc caccatttgg aggtacgata
     5401 ttttcatacg tgtatggatc ataagaaatg gcaggaggcg ttgctccaac agcttctgcc
     5461 gaaggtgaaa agataaatat acttaaaaca aaaagcgaag taaaaaagaa ggagcgaaaa
     5521 aatgaaaatt tattttgtag caacatgttc atcctccttt tcctttcttc tccataacaa
     5581 caaccacgca agaagccata attctatacc aatgatcaaa taaagaaaac ggatcttttg
     5641 ttcattcgtc tccgggaata cgccatgttg attagattct ttataaatcg gtagctgtgt
     5701 ctgctgtttt gatgcgccat tgactgaacc acttggatgc ggaattggac ttagatcata
     5761 aaatgtaatc gtacctgaac ttttggtatc tgcttggact ggctcttgcc agctgaagaa
     5821 cagcaaactt gcaataatcc ctcccaataa gatcgatgtt ttcaacgaaa tcacctcatc
     5881 atgttgtttc tccttttttc tttttcttgt ggacccacaa aaacagcaaa atcaaccata
     5941 acactagaac tgcagcaacc aatcctgcat aaagccatgg gaaagattgt tcttgtactg
     6001 caaaaacgga attttcattt actttttgtg ctttttcttt tttgatcgta aagttttgtg
     6061 taaatcgcca ttcattactg tcgcctgatt ttgctatgat ttctgcgcgg taatcgccgg
     6121 cttttgcttt atcaccagct aaatcaagat tcaagtgaaa attcgaattt ggtgcaacta
     6181 aaaatgcatt agacgtattt tcaatataaa gctgattttc ataaaagatc ttgctcgtaa
     6241 atatcaaatt gctgataatc ctaggctgag gattttggaa acgaagctga acagttggct
     6301 ttgcgttgac ctcgtccaaa tctcctgata aaagtttcag atccggttct atcgtctctt
     6361 gactttcttg taaaacgact gcgagattat aagcaatacg atttttgatt tgtgctcctt
     6421 ctgtagcatt ttctgtttca gcttcttctt taggtgaaac atgaataccc gctaatacac
     6481 gtcccttaaa gctttttgct gggattttca cttctatagg tacagttgtt tctgcatgtg
     6541 ctgatacatt gatttccttt ttcgcaatga gcaaaacgtc acggacatca aacggcaatg
     6601 tctcatctaa cgttgggttt ttttgactat aatcgatcgt tccaccatca ctcgttaccg
     6661 ccggattgac agagacctca aatgttcgtt cttcatctga agaattgctg ataattactt
     6721 ctagtgtctg attttgttcc ggtgccacta ataaatcaaa ataaccgatt gctttatcta
     6781 tttggttctc tggaaatacc ggtttgattc caaagtctcc tgctgaatcc tttgcttggc
     6841 tgattgacgg aaaaatgaaa aatagactta aaatagaaaa aaacgaaaca aacagtcgtt
     6901 tactttgatt cattgtccac cacatccttt caacaacaac tgcggtaaaa aatccttcac
     6961 ctatagtatt ttcaccgcag tttgttgaat ttatttcata ctctatgcta ctgggccagc
     7021 cacgaggttc caagtcaaat cagccgtgaa tgttccagct gctgctttcg gcactttcag
     7081 tgtgatattt gctggatcat aaacagaaag ccatgttccc agaccttgat cttttgctgc
     7141 tgtaaatatt ggtgtcgctg cacttgcttt actcaaccca gttacagttt gactagttgg
     7201 tccagtggat tcgttatctg acgtactttt aacagtggat gttccaaaat ctaattcagc
     7261 gccttgcaaa acttgtgaac cgtttacaaa atctgaaacc gaaactgtca cgttccaccc
     7321 ttgtgcttgg ccatctgcac ctacacctcg acgatctgat acttggagat aaggtgtatc
     7381 gttttttgaa gcgtctactt gatacgtttt tgttccgctt tcaatctcgt gtgtaccaaa
     7441 tggcaattcg ggaactacat ccagtgttaa aggacctgtc tggccagttg ctggattttc
     7501 cggatctgct ggctctactg gttttgaagg atcagtaggg ttaagaggct ccacggtatt
     7561 tgttggttca gtaaaggtaa tatctccttt tgaagtcgct tgaccctcag caaaagctgg
     7621 tgttgccaag ccgcctagaa gaagtgttga aagtaataat gtcccaatca tgttcttttt
     7681 cataataaat cactcctttt tcttagtgac ctaagtatag tagagacagt atgcaaaaaa
     7741 tcctcatgaa ttcatccgct tacttgaaat tatgaccctt tttgtataaa agagaaaaaa
     7801 acaataaaat cgtttacatg aatattcttc cttttttatt aa
//
LOCUS       DAZUKD010000128         7775 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000128 DAZUKD010000000
VERSION     DAZUKD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.131"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..983
                     /locus_tag="ACG8N9_002447"
     CDS             120..983
                     /locus_tag="ACG8N9_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4898000.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1112..2734)
                     /locus_tag="ACG8N9_002448"
     CDS             complement(1112..2734)
                     /locus_tag="ACG8N9_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4898001.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2983..3504
                     /locus_tag="ACG8N9_002449"
     CDS             2983..3504
                     /locus_tag="ACG8N9_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4898002.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3690..5420)
                     /gene="spxB"
                     /locus_tag="ACG8N9_002450"
     CDS             complement(3690..5420)
                     /gene="spxB"
                     /locus_tag="ACG8N9_002450"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4898003.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5585..6403
                     /locus_tag="ACG8N9_002451"
     CDS             5585..6403
                     /locus_tag="ACG8N9_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4898004.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6506..6703)
                     /locus_tag="ACG8N9_002452"
     CDS             complement(6506..6703)
                     /locus_tag="ACG8N9_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898005.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6872..7450
                     /locus_tag="ACG8N9_002453"
     CDS             6872..7450
                     /locus_tag="ACG8N9_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4898006.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7545..7682
                     /locus_tag="ACG8N9_002454"
     CDS             7545..7682
                     /locus_tag="ACG8N9_002454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898007.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2324 a         1391 c         1545 g         2515 t
ORIGIN      
        1 tcacaacctc ttattttgat tagaacttgc gaaaataaac cggttaaagt gtgtctagcg
       61 caagagattt gctataatag acgataaggg taaaatgtaa cgagaagggg ttgaaaaatg
      121 tgtatcaaat tgtaacagat tcttgttgtg atctgccata ccatgtatta gcaaaagaaa
      181 aagttgattt tttatcaatg aatctccaat tgaatgatca agagttagta gatgacctcg
      241 gaaagacatt caattatgac cgctacatga atcagctgaa aaatggtgcg atgcctacga
      301 catcacaaat caatgttggc cgatatttag aatttttccg ttcttatgcg gaaaaacaga
      361 ttcctgtttt atatgtggct ttttccagtg gattaagcgg ctcttacagt agtgcgcttc
      421 aagcggttga attgctgaag gaagaatatg aagaccctga gatctatgtt tttgatacaa
      481 aagctgcaag tattggggaa gggcttttag tattggaagc tgtaagattg aaaaaagaag
      541 gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa gtccattcat
      601 gggtaacagt agacgatctg aaatatctag aacgaggcgg taggatttcc aaagcaagtg
      661 ctactcttgg cagtctattg agtgtaaagc ctattattac ggtggataaa gaaggaaggc
      721 tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat ccagacaatc gtcaatgaga
      781 cggtaaaaac aatcgttgca ccacttgatc aaatcgttta tgtggcttac gctggggatc
      841 tagagagcgc ggagaaggca aaacggcttt tggaagagca gataaagatg aaagatgtga
      901 agctttatcc cttaggacca acgatcgcca gtcacacagg ctatggctgt atcgcgattt
      961 tttcgatggg aatcagtcga taaaagcagt aactcattct gtaaaaaagc ccagaaattg
     1021 attgtaacaa aaacagcgac tcttaaaaga tagatgagca tcctatctct tagaggtcgc
     1081 tgttttttta ttttttaagc taaataggcc attatgccca taactcgttc aattgttttt
     1141 gaatatctgc attttccaaa aattcatcat atgtcgtatc agctcgatcg atcacacctt
     1201 tatccgaaac ggcaatgatg cggtttgcca atgtttggat aaattgatgg tcatgtgacg
     1261 caaataagat tgagccagta aatgccatca agccgtcatt caatgctgtg attgattcta
     1321 agtctaaatg gttcgttgga tcatctaata caagtacgtt agctttcgaa agcatcattt
     1381 ttgaaagcat gcaacggact ttctctccac cagataaaac attcactggt ttcaatactt
     1441 cttcaccaga aaacaacatt cttcctaaga agcttcttaa gaaggtgtta tcatcttctt
     1501 ctttacttgc atattgacgc aaccagtcta aaatcgttag atcagtatcg aattctttcg
     1561 ttgtatcctt tggtaaataa gaattacttg ttgtcacacc ccaacgtact gttccagtat
     1621 ctggcgtaat ttcacccatg ataactttga atagcgttgt tgttgttata tcattttctg
     1681 cgacgaaagc gaccttgtct tctttgctca aattaaaaga tatatcatct aaaattttct
     1741 ttccatcaat cgttacactt acattttcta cctgcaataa gtcgtttcca atctcacgtt
     1801 caggtttgaa tccaacgaat ggataacgtc tagaagaagg ttggatatcg tctaaagtaa
     1861 ttttttcaag cattttttta cgtgaagttg cttgttttga ttttgatgca ttcgcactaa
     1921 agcgggcaat aaattcttgg agttctttga tttgttcttc ttttttggca tttgcgtttg
     1981 cttgaagttt agccgctagt tgactagatt ccaaccaaaa atcataattt cctacataaa
     2041 gtttgatttt accaaaatct agatccgcca tatgtgtaca tactttgttc aaaaagtgac
     2101 ggtcatggga tacaacgatc acagtatttt caaaattgat caaaaattct tccagccaat
     2161 tgatcgattg gatatccaga ccgtttgttg gttcgtctag caaaagaaca tcaggtttac
     2221 caaatagtgt ttgtgctaat aacactttta ccttttgtcc accagttaat tcactcattt
     2281 gctgatggtg caattcttca gggatgttca gaccttgcag taaaacagct gcttctggtt
     2341 cagcttccca tccattaagc tcagcaaatt ctccttctaa ttcagctgct cgaataccat
     2401 cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc acttcataca
     2461 gacgcttgtg tcccatgatg actgtttcaa gtacagtata gtcttcatag tcgtaatggt
     2521 tctgtttcaa tacggccatc cgttcatctg gtcccatagt aacggaacct gtggaaggct
     2581 gcagatcccc tgataacact ttaaggaacg ttgatttacc tgcaccattt gcaccaatca
     2641 agccatagca gtttccgggg gtaaatttaa tattcacatc atcaaatagt ttgcggtctg
     2701 aaaagtgcaa actcacatca tttacagtaa tcaatttttt tcctcacttt tatcattatt
     2761 agttttgaca agtcttatag aattatatag gggaaaggta aaagattcaa ggcaatcgaa
     2821 cattctttgt gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc ggagcgaaaa
     2881 tagctactat aggatttatt agtttgcaac agcataattt ttgacgttat ttataagagg
     2941 gagtactata aaactgtaaa agaaatgaaa gaaggattga aaatggatga aaaaagaacc
     3001 atcgactggg gctcattgat tttgggaatt ttatttgtac ttgtctcatt gttatcattt
     3061 caagaccctg tcggaaattt agttgcaatt gtagtagtct ttgcgatttt tgcctttatt
     3121 aaagggatat ttgaactgtt tgtacggaac agattaaaag agctaacagg atataaagga
     3181 aagatgccct taataatcgg gatcatcgat attctagttg gcgtcttttt cttattcaat
     3241 attggtgcag gagtcgcagc attgcctttt gtttttgcag tatggtttat tgctgattca
     3301 gttttagcgt tatttactgc tgacttggca agaggagtaa gtgaaggata ttattggttt
     3361 acgatcatcg tcaatatttt aggaatttta ttaggaattt tcttattgtt taatccaatt
     3421 tcttctgcgt tgacattaag ctttttagtt ggattctatt ttatgttatt tggtatcaca
     3481 catatcgtct atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg gcttccgatt
     3541 acagttttac tgttgtaatt ggaagccggc ttttttatca tggacaaata tgcacgaggt
     3601 gatttttagg tgtcgagaca aatttatgca aaaatgtgag aagctatata cacaaatttt
     3661 atcttaatac tcgaagcata gcgcagttgt cacaacctct tttcatataa ttcattcaat
     3721 actggcaaat catggacttc atatttttct ttgaatgctt ggatttcttc ttcagaatat
     3781 tttcttggat ctaaatgcag atcttctact ggcaatgggc gcttgttatt gatcttcaca
     3841 tcgattacta caggacgatc tgattttgca gctgcatcaa aggcaaatcg aagttgcgag
     3901 tattctgtga ctgtaaagct atttgccccg agcgcttctc caactttacc gaaatctgca
     3961 tcttctaaaa atacaccaaa tttcttttgt tccgtatctt cttgttctgc ttcgatgaaa
     4021 ccaaaagaat cattcgaaaa gacaacatta ataattggaa gctgatattt gacttgtgta
     4081 atgatatctt gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa tacttgtttt
     4141 tctgggtagc ttagttgtgc ggcaattcct cctggcacac cattacccat agttgcgaac
     4201 catccagatg tcgtatgttt ttgtttaccg ttcatattca ataaacgaat agaatggata
     4261 gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg attgatttct
     4321 ttatagacag cctctggacg aatcggttct tcctctcgat cttcaaaaga tttcaaccaa
     4381 tttacccatt tttctttatt ttcttgattt gctaggtacc agctatcagc agggcgagca
     4441 tcccctaact cgacaagtct ttgcaaagct gtgtttgcat caccgaggat cgacaaagat
     4501 gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga tttggataaa ttcagcagtt
     4561 ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga caaataagat cagatcggct
     4621 tctgccaatg cttcatttcc gggtttagta gcgacacgtc cagcaaaacc aagaaagttt
     4681 tcataatcat caggaataat tccttttgct agaacggaag cgacaacagg aactgaaaaa
     4741 tgttctgaga attctttgat ttgcggaaat ccgtttcgcg ttccttgacc aacataaaga
     4801 actggttttt ttgctttttc aagataaggc aaagcagcta gcaaatcttt ttcttctggt
     4861 aatatcacac ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc aatttcttca
     4921 aaaccaagat caacagggat agtcacgata gccacgcctt tatgctcata ggcggcttta
     4981 atggcttcat ctactacatg cggaagactt tgtggtgtca tgactgtacg attgtatacg
     5041 ctaacatctg cgaaaatagg attttcattt agttcctgga aagagttata gttcatggat
     5101 gtagaagcaa cttgtcctaa gaacgctact agaggcacat ggtccatctg tgcatcatac
     5161 aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc gccgactttc
     5221 cccgttaatt ttgcatctgc agcggctgcg agtgcgccga cttcttcatg acgaacttga
     5281 atgtagttga tttcggtttt ttcttgatat agcgcatcca taattgaatt gaaagatcct
     5341 ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa gaacttttac cattgcgatt
     5401 cctgcattga tttttgacat ggtgtttcac tcctttagca atgatgatac ataaattttt
     5461 tgtatctaga gtatagaact ttttatcgaa aatcccaact aattcgtctt ttcacaaaaa
     5521 tgtagtcatg tttttgggaa tatgctagag tagtacatag atagattcaa gagaggggaa
     5581 tggcatggtt gaatggctat tgagacttga accttggcag caagcacttg ctgggacagc
     5641 ttttacttat tttatgacag ctcttggtgc cgggttggtt tttttcttta aagaaatcaa
     5701 aaaagaagtg ctaaacttga tgttaggatt tgcttctggt gtcatgattg ctgcaagttt
     5761 ttggtctttg ttagatccgg ctattacaaa agcagaagaa aatggagata ttgcttggct
     5821 agttgtaagt attgggtttg gtttaggcgg tgtgttttta tatatggcag ataaaacact
     5881 gccccatatg cattttggac cacaacatga agcagaagga ttgcctaccc atttaaaacg
     5941 gacgatcttg cttgtttttt ctattacatt gcataacatt cctgaaggtt tagccgtggg
     6001 ggtagcattc ggtgcagcag caactgcgga taatccgaca gccgctatat tagccgctgt
     6061 ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa ggagcagctg tatcaatccc
     6121 gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag cttctggaat
     6181 cgttgaacca attgctggag tgattggagc gctactagta acgaaagtgg agctgctgct
     6241 tccttatgca cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg aagaattgat
     6301 ccctgaagca cagcagacat tgtccagcaa acgtcatttt gcagtttttg gcgtcatgag
     6361 tggatttatc attatgatga ttttggatgt ggcattagga taaataaaaa aaatatatat
     6421 gatatatcaa atagggccgc gaacttatat cgcgacccta tttttttagt aagttatatc
     6481 ataatgaagt gaatggtatc atacattaat aatagttttt tctatgtgta tttcctttga
     6541 tccagcgaac aagccagatg ataagtgcga ttgggattag aactttgacc gcaaaccata
     6601 aaagcccgct taggacactg acaaaaagac tgatgacgaa gctagctgca attagcacta
     6661 aaagaaccat gaagatattt aagaacatat ttttttgacg catacaaaac cctcctcttg
     6721 ttcattcttt actatagaaa aagtataaca gggattgttt aaaaacacct ccaactgaag
     6781 actgattttt acttcatcta cttaaatgat caaacaattg ctttttctct ggtttatcat
     6841 ctaaaataga gcggaaaagg aggagaatat catgcctaaa aatcgtcttg aagcatttac
     6901 agatgctgta atcgcgatta tcatgactat tttagtttta gaacttcatg aacctaacgg
     6961 agacacgtgg gaggcgctcg ctgcattagg ttaccgattt tttatctata ttgtcagttt
     7021 tatcatttta gcgatttatt ggaacaatca tcatcattta tttcaagttg tacataagat
     7081 aaatggacgg gtcttatggg cgaacagttt ctttgttttt gcgctttcgc tttttccgtt
     7141 tgcaacttca tggatcagtg agtacatcaa tagcctggct ccagaaatga cttatggttt
     7201 tgttaccttg accgctaata ttgcgtatta tatcttggca aaagaacttg tcaaagcaaa
     7261 taaaggccac gagaaagcat tgaaggcgat gttgaataat tacaagaaat cgtatctatc
     7321 gattggtttg aatgtgttag gaattgtctt agggtattta gtagcgccag tagccgtgct
     7381 gatcgcaaac atcgtgattc tagctgcttg gatcatccca agtcgtacca tcgaatcaca
     7441 atatcggtaa aatgatccgt gtttaaaatt taagagaaaa atatagaaaa aataaataaa
     7501 cgatcaaata tccgagtgat actagtattc aaagagggat gcagatggat caaaaaagaa
     7561 ttcatgtagg actgattact agttttgtca cgatgcttat ttatttggat caaggtcatt
     7621 tgacttggac agtagttggt gtcgtaggat ttttgacatt attcacagca ataaaaaaat
     7681 aaacatcttt atgtaatctg atgttctatc tatttaagaa aagcatgttt acagcagtat
     7741 gattctgttg taaacgtgtt tttatttgtg gaaaa
//
LOCUS       DAZUKD010000129         7488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000129 DAZUKD010000000
VERSION     DAZUKD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.132"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1392)
                     /gene="eno"
                     /locus_tag="ACG8N9_002455"
     CDS             complement(94..1392)
                     /gene="eno"
                     /locus_tag="ACG8N9_002455"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4898008.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1508..2263)
                     /gene="tpiA"
                     /locus_tag="ACG8N9_002456"
     CDS             complement(1508..2263)
                     /gene="tpiA"
                     /locus_tag="ACG8N9_002456"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4898009.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2367..3557)
                     /gene="pgk"
                     /locus_tag="ACG8N9_002457"
     CDS             complement(2367..3557)
                     /gene="pgk"
                     /locus_tag="ACG8N9_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4898010.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3667..4668)
                     /gene="gap"
                     /locus_tag="ACG8N9_002458"
     CDS             complement(3667..4668)
                     /gene="gap"
                     /locus_tag="ACG8N9_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4898011.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4709..5800)
                     /locus_tag="ACG8N9_002459"
     CDS             complement(4709..5800)
                     /locus_tag="ACG8N9_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4898012.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5949..7283)
                     /gene="rpoN"
                     /locus_tag="ACG8N9_002460"
     CDS             complement(5949..7283)
                     /gene="rpoN"
                     /locus_tag="ACG8N9_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4898013.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7343..7414
                     /locus_tag="ACG8N9_002461"
     tRNA            7343..7414
                     /locus_tag="ACG8N9_002461"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7376..7378,aa:Arg,seq:ccg)
BASE COUNT         1939 a         1581 c         1385 g         2583 t
ORIGIN      
        1 atatttcagc aagtagagag ttcgattact taactaagtg atcatactcg actatctttc
       61 aatagtcgat catgtatctt cattagaaaa gaattatttg ttttttaagt tgtagaaaga
      121 tttcaaacct ttgtattctg caacttcacc aagttggtct tcgatacgta gtaattggtt
      181 gtatttagca atacggtcag tacgtgatag agaaccagtt ttgatttggc cagcgtttgt
      241 tgcaacagcg atatcagaga ttgttgaatc ttctgtttca ccagaacggt gagatacaac
      301 tgctgtgtag ccagcttctt tagccatttc gatagcttcg aaagtttctg ttaatgtacc
      361 gatttggttc actttgataa ggattgagtt agcaattcct ttttcgatac cttcagataa
      421 tttttgagtg tttgttacga acaagtcgtc accaactaat tgaactttgt cgcctaatac
      481 gtcagttaat ttcttgaatc cgtcccagtc gttttcgtct aatccatctt cgattgagat
      541 gattggatat ttagaaacta attcttcgta gaatttgatc atttcgtcag ttgttttttc
      601 gccttcgcct gagtcagcta gtacgtaaac acctttttct ttgtcgtaga attctgaaga
      661 agcagcatcc atagcaagaa caacgtcttt accaggtaca tagccagctt tttcgattgc
      721 ttcgatgatt acttcaaaac cttcttcgtt tgaaccaagg tttggagcaa atccaccttc
      781 gtcaccaaca gaagttgcta atccgcgagc tttcaagatt gaagctaatg cgtggaatac
      841 ttcagcaccc atacgtagag cttctttgaa tgttggagcg ccaacaggca tgatcatgaa
      901 ttcttggaag tcgattgagt tatcagcatg tgatccgccg ttgatgatgt tcatcattgg
      961 tgttggcaat acttttgtgt tgaatccgcc taggtagtgg tataaaggta cttctaggta
     1021 gtcagcagct gcacgtgcaa cagcgataga aacacctaaa atagcgttcg cacctaattt
     1081 acctttgtta ggagttccgt ctaaagcgat catagctttg tcgatagcca tttggtcacg
     1141 aacatcgtag ccaatgattg cttcagcaat gatgttgttt acgttgtcga cagctttagt
     1201 tacaccttta ccgccataac gagctttgtc tccgtcacgt aattcaactg cttcgtattc
     1261 accagttgaa gcacctgaag gaaccattcc gcggccaaaa gctccgcttt ctgtgtatac
     1321 ttctacttcg attgttgggt taccacgtga gtctaagatc tcgcgagcgt aaacatcagt
     1381 aataattgac atgttttgtc tctcctttga gtttgttttt tcctaaggga tttccctcat
     1441 atacgatttt agttaatttt gctcattagt gcaaacattt cttgcatttt tcgcaaattt
     1501 tcaacaatta ttttacagcg tctaacaatg acaagaatga atctgcttgc aagcttgcgc
     1561 caccgactaa agcaccgtca acattttctt ttgccatgta ttcagcaata ttttctggtt
     1621 ttacagaacc gccgtattgg atacgaactg cttctgatac ttcttttcca taaagttttt
     1681 caactgtttg acgtacaact ccacaaattt cgtcagcgat ttgtgcgtca gctgatttac
     1741 cagtaccgat tgcccagatt ggttcataag caataaccat gtttgacacc tgttcagctg
     1801 aaagatcagc taaaccagct gtaatttgtc cttcgatcca ttcagcagtt ttgcctgctt
     1861 cataggtttc taaagattcg ccgcagcata ggattggtgt cataccattt gcaaagatag
     1921 ctttcgcttt tttattgatg tcttcgtctg tttcatggaa atattcacga cgttctgaat
     1981 gtccgatgat cacataatga acacctagat cagcaagagc tgctggtgaa gtttcaccag
     2041 tgaacgcacc tgagttttcc cagtagcagt tttgtgctga gatttttagt tctgtacctt
     2101 cagctgatgt cattaattct tgtaagaata atgcaggaga tccgatgact gaatcaactt
     2161 tgtcatttga agggattttg ttttttactg cttcagcaaa ttcttttgct tctgatgcag
     2221 ttttgttcat tttccagtta cctgcgataa taggtttacg cattgaggaa cacactcctt
     2281 aatttattga aaaatggcta gggagtcatg aaaactccct tcaccttttg ttcgattact
     2341 cgtcagaatt gagcaaactt ggtcgtttat ttatcgttga ttgctgctag tcctggtaat
     2401 tctttacctt caagcaattc caagcttgcg ccgccacctg ttgagatgtg tgtgaatttg
     2461 tcagcaaatc ctaattgttc tgctgctgcg gctgagtctc cgccaccgat gattgttgtt
     2521 gcgccttcta agttagcgat tgcttcacaa acaccgattg tacctttagc aaagttactc
     2581 atttcaaata cacccatagg tccgttccaa acaactgttt ttgcaccttg taatgtatca
     2641 gcaaataatt ttactgatgc tgggccgatg tctaaagcca tcaaaccatc aggaatgtct
     2701 ccttcgatta cttgtgtttc aacatcattt gaaaattctg gtgcacagac attgtcgatt
     2761 ggcaagacaa gtttgtcgcc tgctttttcg attaattctt ttgctagttc cactttatca
     2821 gcttctacta gagagttacc gatcttgatt ccttttgctt cgtagaatgt gtaagtcatt
     2881 ccgccaccga tcaagatttt atctgcttta ggaatcaagt tttcgattac gccgatttta
     2941 tcagaaactt ttgcgccacc aaggattgca atcattggtc gttttggttc ttctactgct
     3001 tcaccgatga acttgatttc tttttccatc aagaatccag caactgttgg gataccagtt
     3061 gaagcgatac ctacgttaga agcatgtgca cggtgagctg taccgaaagc atcgttaaca
     3121 aatacatcgc ctaatgaagc ccagtattta cctagttctg tatcattttt gctttctttt
     3181 ttaccgtcaa tatcttcaaa tcgtgtattt tcgaatacga caacgtcgcc gtcattcatg
     3241 ttgcggattg cttcttctaa ttgttctccg cgagtttcag gtacgaatgt tacttcttta
     3301 cctaataatt cgcctagacg ttttgcaact ggagctaaag attttccttc tttatcttct
     3361 tctgttttta cacgtccaag gtgagagaaa aggattgctt ttccgccttg ttctagaacg
     3421 tagttgattg ttggcaatgc tgctttgata cgagtgtcgt cagtgatcac gccgtctttc
     3481 aaaggaacgt tgaagtcaac acggacaaga acttttttgt cttttaaatc gatatctttc
     3541 actgttttct tagccatttt atttcctcct gaataaactt attatcaaaa aaagcggaag
     3601 aagcgcgatt gcttccccgc ttttaaatgt acatgtctat aatttacact tgtacgtttg
     3661 tctatcttat aagttagcga agtattctaa agtacgtact aattgtgcag tgtatgacat
     3721 ttcgttgtcg taccaagcaa cagttttaac taattgtttg tcgccaactg tcattacttt
     3781 agtttgtgta gcatcaaata atgaaccgta agtcatacca acgatatctg aagaaacgat
     3841 ttcgtctgtg ttgtatccgt aagattcgtt tgaagcttct ttcattgctg cattgatttc
     3901 gtcaacagtt acttcttttt caagaactgt tactaattct gttaatgaac ctgttggtac
     3961 aggaacacgt tgagcagctc cgtctaattt accgttcaat tcgggaatta ctaaaccgat
     4021 agctttagca gcacctgttg agttaggaac gatgtttgca gcagcagcgc gtgcacggcg
     4081 gaagtcacct ttaggatgag gtccgtctag agtcatttgg tcacctgtgt aagcgtggat
     4141 agttgtcatt aatccttcaa caacaccaaa tttgtcgttc aatgttttag ccataggagc
     4201 taaacagtta gtagtacatg aagcacctga gataactgtt tctttaccag ttaatgtttc
     4261 gtggtttgtg ttataaacga ttgttggaac atcgtttccg ccaggagcag agataacaac
     4321 gcgtttagca ccagctgtta aatgtttttc agctgctgat ttagaagtga agaaaccagt
     4381 acattctaga acgatatcta cgcctagttc gccccatggt aattcttctg ggttgcggtt
     4441 agctaatact ttaacttctt ttccgtttac gttgaatgaa ccttcatgaa cttcaactgt
     4501 tccgttgaaa cgtccttgag ttgtgtcgta ttttaacaag tgtgccaaca ttttagcatc
     4561 tgtcaagtcg ttgattgcta ctacttcgat tccatctaca tcttggatac gacggaatgc
     4621 tagacgtcca atacgtccaa aaccattaat acctacttta actgtcattt aagatttcct
     4681 ccttatgaaa atcaaaaaaa tatttatttt aaagggttac cccttttaaa atctcatttg
     4741 cagcagcttc atcagtgatg agccacgttt gttttggtgc atttttcata taagctcgaa
     4801 ttgctttagc ttttgatttt ccgcccgcga ttgccatgat gatcgggact ttttccaaat
     4861 cttgcaattc tatgccgatt ctagggacct tataaacgac ttcacctttt tcattgaaaa
     4921 agtagccaaa agattccgca acagcatttg cttgtttcag catgactaat tctttttcag
     4981 tcatcttacg acgtgctgcc atgtgtaatg cacgcccaat actatgaata acacaattcg
     5041 cttcactaat caagtttaag acttcttgga tagatggctc attgagtaaa gaattgtaag
     5101 ttgccagact taattgttcc ggaacataaa gtgcgcggtg gttaccgttt gctttagcag
     5161 ccattcttgc actgacagaa tttgcctgta cagttatcgc ttcaccaatc ccgcctcttg
     5221 cagggacaaa caggttatgc cgtttcttat tttccagatt agacaattgt tcggctacca
     5281 cagccatagt agttcctccc atcacagcga tgatattctc gccttctgga agtagtcggt
     5341 ctaaggcttc gctcagcgct tcgccgaatg catcagccac tttggtctgc tcttcgctgt
     5401 ttcctgaaac aacgatacag cgttggttac caaaatattc tgccaactgt tgttcggttt
     5461 gatgcgtacc taaaagctga tccatgaagt tttcaaggct ttgataaact tcttcgccct
     5521 tagttgtcag cgtcataccg ctttttgagg tgtcgatcaa attcagattt ttcaaaaggt
     5581 ctgtttctgt tcgtaagact cgttctgtga tccccatact ttccgataaa cttcgacgcc
     5641 caatcggctg catccaatag atatttcgta aaattttata gcgttcttgc aacacttcga
     5701 taatatctgg tgcaacagat tcaatcattt ttatttcatc cagcatagcg atctccttag
     5761 ttggacactt tacgtccaat gtagacttaa aacgacccat atacgataaa aaaagaaatt
     5821 aactattcga atttcacatc aataaaggga aatctcaacc tcacgactgt aagtctaaca
     5881 gttttgctct cgtttttcaa ctaaaaagac tagaaaaagt gaaatacttt ttctagtctt
     5941 ttgactgatt agttatcata acgttttctt tttgatgaac tcgggattcc taataaatca
     6001 cgatattttg ctactgttcg tcttgagatc gctatctcat cttttttcag caattcaacc
     6061 aatttctggt cagacaacgg cttgtttttg tcttcttgat cgactaattc ctgcagcttt
     6121 ttcttcgctg tatctgctga aaggtcttct gtctgttctg tactattcgt aggaattcgt
     6181 gtagtgaaaa atttttttaa ttcaaataca ccgaagtctg tttccaaata tttcccgttg
     6241 actgcacgac tgaccgtgga ttcatgcaca tctatctcag ttgcaacttc ctttaacgta
     6301 agaggcttga ttggacgttc cttgtcgata aagaaagctt tttgccggga aacaatgact
     6361 tgagcaaccc gcaaaattgt atccccacgt tgtaggatcg ttttcttcaa ccattcgaat
     6421 tcctgtttcc gttcttttaa ataattttca gtttctttat ctgcttgttg tgacatttgt
     6481 ttgaagtaac tctcttgaaa gcgtatattt ggctgatttt ttcggtttga gataacttgt
     6541 acatttttat cgtcgtccac taatacccgg acatcaggaa taatgtacaa ttcgctgcta
     6601 cggtcgaaaa tccggccagg gcttggactc aacgtttgta aatagtcgaa tatctgctga
     6661 acagcatgta gatcaacaga aaaacgttgt gcgatttctt tccattttcg ctccaccagt
     6721 tcctcaaaac attcttccaa cacgatataa gctaattccg gcgcataatc atctcgttct
     6781 gtttgaagca tgagacattc ttgcaaacta cgtgcaccta ctccagctgg ttctaattgc
     6841 tgaatcagag tcaacgcatc gagcatttgg ataggcgtag ctccaatctc ttttccggct
     6901 tcttctaaac taatcgtcaa aaatccgttc aaatcgatgt attctacaag ataaagaacg
     6961 attttcctca aaaaagtatc tcggtaattc aaatggactt gttcgatcaa tgaatcaaat
     7021 aaagaccctt ttgtatcagg aatctgattt aggtaatttg tctcttctcc ttcataagaa
     7081 gatgaagaag cttttatgta atctggctgc cattccggct caacaacttc caccaatgga
     7141 ttttccattg cttgattctc aacgaatgcc aacagctcct cactgttata ttgtaaaact
     7201 tggatggact gttgcaactg ttgcgtcatg gctagtttct gtgtctgctg ctgtttttgt
     7261 gaaaaatttt gctcaaattt catttttttg tccaatcccc ttgtaatttt ttatctgttt
     7321 agttacaatt atcccagtgc ttgcgtcctt agtgtagtgg atatcacgta agattccggt
     7381 tcttgagacg ggggttcgat tccctcagga cgcgttctac tgctatttta gcataattta
     7441 cgaatagttg cttctttatt tttaagtgaa aactgtccgc caaagaaa
//
LOCUS       DAZUKD010000130         7149 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000130 DAZUKD010000000
VERSION     DAZUKD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.133"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACG8N9_002462"
     CDS             185..1183
                     /locus_tag="ACG8N9_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4898014.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACG8N9_002463"
     CDS             1295..1804
                     /locus_tag="ACG8N9_002463"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4898015.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACG8N9_002464"
     CDS             complement(1834..2493)
                     /locus_tag="ACG8N9_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4898016.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACG8N9_002465"
     CDS             complement(2495..3259)
                     /locus_tag="ACG8N9_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4898017.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACG8N9_002466"
     CDS             complement(3267..3944)
                     /locus_tag="ACG8N9_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4898018.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACG8N9_002467"
     CDS             complement(3981..5366)
                     /locus_tag="ACG8N9_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4898019.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACG8N9_002468"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8N9_002468"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8N9_002469"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8N9_002469"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4898020.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..>7149
                     /locus_tag="ACG8N9_002470"
     CDS             6305..>7149
                     /locus_tag="ACG8N9_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898021.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLL"
BASE COUNT         2133 a         1444 c         1227 g         2345 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttca
//
LOCUS       DAZUKD010000131         7130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000131 DAZUKD010000000
VERSION     DAZUKD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.134"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..398)
                     /locus_tag="ACG8N9_002471"
                     /pseudo
     CDS             complement(1..398)
                     /locus_tag="ACG8N9_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            477..653
                     /locus_tag="ACG8N9_002472"
                     /pseudo
     CDS             477..653
                     /locus_tag="ACG8N9_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(829..1398)
                     /locus_tag="ACG8N9_002473"
     CDS             complement(829..1398)
                     /locus_tag="ACG8N9_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4898022.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1472..2761)
                     /locus_tag="ACG8N9_002474"
     CDS             complement(1472..2761)
                     /locus_tag="ACG8N9_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898023.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3088..3963
                     /locus_tag="ACG8N9_002475"
     CDS             3088..3963
                     /locus_tag="ACG8N9_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4898024.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            3977..4300
                     /locus_tag="ACG8N9_002476"
     CDS             3977..4300
                     /locus_tag="ACG8N9_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898025.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4297..5049
                     /locus_tag="ACG8N9_002477"
     CDS             4297..5049
                     /locus_tag="ACG8N9_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4898026.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5039..7130
                     /locus_tag="ACG8N9_002478"
                     /pseudo
     CDS             5039..7130
                     /locus_tag="ACG8N9_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2107 a         1419 c         1290 g         2314 t
ORIGIN      
        1 tttgcgtttt cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc
       61 gtagaatttg cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt
      121 ggattatcat ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc
      181 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
      241 aaatgcgatt tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat
      301 ttcattgctg aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct
      361 ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta
      421 ctctacatct tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt
      481 cagctagaac gagacaagca ggaatacctt tagaagatat caaagatttt ttaggtcata
      541 aagatgtttc cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa
      601 tgaatcaact agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt
      661 tttaagctca ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat
      721 cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg
      781 gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg
      841 tagaaaaatt ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc
      901 tatgatttca gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt
      961 cccagtttcc atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt
     1021 aataaaacct agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt
     1081 aatgagcgag tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac
     1141 ggaagctgtt ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt
     1201 cactgtttga cgaaggacat ctagttcttg caagagcgtt ttgtattgtt cttgggtcag
     1261 catcacgcca gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc
     1321 ttgagcaaat acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc
     1381 taatttcttt aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga
     1441 ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga
     1501 tatcggtaag aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt
     1561 cgttatggtc tagtggaaaa aaatgatcgg tgatcactag atctgcatac cgaggcggct
     1621 tatttcgagt acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta
     1681 ccccagcaag tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt
     1741 caggagggtg gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc
     1801 actctactgc ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat
     1861 ctttggaatt gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc
     1921 ggtcatggat accaattacg taacgaagcc attcttggta aagtactttt atcagctttg
     1981 atttgctttc gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca
     2041 caatcgtcaa tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa
     2101 tctggttaca gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct
     2161 gctcctctct aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta
     2221 aaatctcgat ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa
     2281 aggactgctc tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag taaaacagca
     2341 gatatcttac ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt
     2401 ggtactcgcc aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt
     2461 aaggttcatt taatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa
     2521 caaacaagag ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat
     2581 gaagaatcag ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg
     2641 ttttaacatc gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca
     2701 ttagattgat tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca
     2761 tcgatcattt ctcacctgat ttctaaacat attgtttgat caaaatgagt tcactcctaa
     2821 aaagataaaa aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta
     2881 ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata
     2941 agatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta
     3001 tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag
     3061 ttgatataag aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc
     3121 gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg
     3181 ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact
     3241 cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag
     3301 tttgaatttc gtccgggacc gatctttacc acgatcttgt tggcagatga gatcaatcgt
     3361 acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc
     3421 gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc
     3481 gagtacgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta
     3541 caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa
     3601 gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat
     3661 gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca
     3721 acaagaagtc atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt
     3781 caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg
     3841 caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag
     3901 gaagacgaaa agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg
     3961 tagattgagg tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct
     4021 ggtatatttc atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt
     4081 gttggccttt tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa
     4141 gaaactttcg gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt
     4201 tattttttcc atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg
     4261 cttcccagca atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa
     4321 gaagtccaat tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt
     4381 tttacagcct atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt
     4441 cctgtgactg ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg
     4501 gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct
     4561 tatcgtccag gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag
     4621 tggttatacc gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg
     4681 ggttcgccca gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg
     4741 agaaaagaaa agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac
     4801 cccggtcata cctattttgc ttctttggaa gctggcgatg agaaagcttt tttcgcacac
     4861 ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc
     4921 tcagaagcta ttgaaacatt gtcaaaagat cgaacggctt atcttgttta tttcgtagaa
     4981 aaacaactca tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt
     5041 gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg
     5101 tgctccgata tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat
     5161 tggtgtccta tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc
     5221 tttgccagta atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt
     5281 atggatctta cagttttttt cgtcagttac agaggaattc ttccatctat cccaaggtaa
     5341 actttattat ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt
     5401 tattacattg accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta
     5461 cttcatgatg cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt
     5521 aactgttttg ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcggg
     5581 aaacactcga ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc
     5641 tatcttgcct ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat
     5701 ccgagaatat cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg
     5761 aagtgcaaga acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa
     5821 tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa
     5881 aaatcattac accggttctg gatggataga agatcaggac gcacccatcc aaccaatcca
     5941 agaattccct gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac
     6001 attgacccta aaaaaacaag ctttcttacc ttatccttat ggaaatacta ccttgactga
     6061 tttttcatta gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc
     6121 tgtcccgaat gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag
     6181 agaagaatta aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct
     6241 tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa
     6301 tgaagcaact ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa
     6361 tttccgctat tccaaaacgg ataccgggca tactccgaaa gaacacgatt atgtggatta
     6421 tttcctcttt gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct
     6481 tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac
     6541 aggcacagat aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg
     6601 gccagaggta tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc
     6661 caatccaatg ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga
     6721 acaaccaaat gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat
     6781 tccatcagaa gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt
     6841 gtgtttatcc gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag
     6901 ttatcttatc tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct
     6961 taaccctttg gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct
     7021 gcgaatagaa caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga
     7081 agcagaaata tatggacgac aagcagatcc ggtaaaattc gataaaaaaa
//
LOCUS       DAZUKD010000132         7127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000132 DAZUKD010000000
VERSION     DAZUKD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.135"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACG8N9_002479"
     CDS             <1..525
                     /locus_tag="ACG8N9_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS4898027.1"
                     /translation="SEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2046
                     /locus_tag="ACG8N9_002480"
     CDS             586..2046
                     /locus_tag="ACG8N9_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4898028.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2057..4261
                     /gene="topB"
                     /locus_tag="ACG8N9_002481"
     CDS             2057..4261
                     /gene="topB"
                     /locus_tag="ACG8N9_002481"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4898029.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            4277..4426
                     /locus_tag="ACG8N9_002482"
     CDS             4277..4426
                     /locus_tag="ACG8N9_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898030.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4500..5093
                     /locus_tag="ACG8N9_002483"
     CDS             4500..5093
                     /locus_tag="ACG8N9_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4898031.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            5109..6008
                     /locus_tag="ACG8N9_002484"
     CDS             5109..6008
                     /locus_tag="ACG8N9_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4898032.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            6011..6496
                     /gene="ssb"
                     /locus_tag="ACG8N9_002485"
     CDS             6011..6496
                     /gene="ssb"
                     /locus_tag="ACG8N9_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4898033.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            6862..7122
                     /locus_tag="ACG8N9_002486"
     CDS             6862..7122
                     /locus_tag="ACG8N9_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898034.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2625 a         1215 c         1483 g         1804 t
ORIGIN      
        1 tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa gaatattgtg
      241 cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt
      361 ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac cgaaaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca atttaaagca
      841 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      901 gaaacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ccgaactaaa gccaaaatat gaacggcaaa atttcttagt tcgagacgat
     1081 cgtttagatc atgcggtcgc ctttttatgg aaagatccac agacaaaaga gacagtaggg
     1141 gcgagttatc aaggaacgtt cattgattat gaacgttttg gtgagagagg cacatataag
     1201 catatagata aaaattcgac agcgaatcat ggctttaacc taaagatcgg tgaccctaag
     1261 cagttaaaat tttttgagag tagtattgat ttacttagct atgctgcact gaatcgtgat
     1321 cagttaaacg atacgtggtt agtctctatg gaagggttaa aacaccatgt gatcagtcat
     1381 tattttggtg aggcagtatc tgaattgaga aagaaacaag cctttcctca atccatcgag
     1441 atttgtgtgg ataatgaccg tgcaggacat attttctacg aaaaagaaca attaatgggc
     1501 gcagtcgatc catttacgaa tcagaaagta cgttgtgaac gaggaatcgc taatgattgg
     1561 caagtgccaa aagaatacaa aatcatttat gaagaagtcg caaaagaaga aaaggtcaca
     1621 ccagaagcta ttatggccat acacaagaca gaaaacaatc tgcaattaac gaaccaactg
     1681 gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa cgattcaata
     1741 gaagccatta atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa agtttgtgaa
     1801 cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag aaaaagggga tattaatgcg
     1861 cagattcttt tctcttataa agcagaacaa tactacaaag gctataaaaa tcatgaacat
     1921 gaatttatac ctgaagtaaa aaaggactgg aacgatcaat tgaagcatga gatccagcaa
     1981 caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca acaagaaagg
     2041 gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aagccgtcac aagcgaaagc
     2101 ttatgccgat agcttctcta aagccactcg aaaagacggg tattttgaaa tccaagatcg
     2161 attgtttcct ggtgaaactg tgatcacgta tggttttggt catttagtcg aattagattc
     2221 gcccgatatg tacgatgaaa attggaaaca atggtcatta gaacatttgc ctattttccc
     2281 tacacattat cactatcatg ttcctaaaga taagaaaaag caattcaagg tggtgaaaca
     2341 gcaacttcaa tcggccgaca ccattattat tgcgacagat agcgatcgtg aaggggaact
     2401 gatcgcttgg acgatcattc aacaagtagg cgccgatcaa ggaaaaacct ttaaaagact
     2461 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccagcagt tacgagacgc
     2521 aaaagagacc tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat
     2581 tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     2641 cttttcctta ggcagagtcc aaaccccaac gctttacatg atttatcaat tgcaggaaaa
     2701 gattagaaat ttcaagaaag aaccttattt tgaaggaaaa tccgaagtaa tcgctcaaaa
     2761 tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga
     2821 agattattta aaagaaaagg gggttcaatt gggaaaacag ccaggcatga ttcgccaagt
     2881 tgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt tctctatcca gccttcagtc
     2941 taaaatgaat cagctcatga aagctagtgc caaagacact ttggaagcca tgcaaggctt
     3001 gtatgaaggg aaatatctaa gctatccacg aacagacacc ccttatatta cggaagggga
     3061 gtatgcgtat ttgttggatc atttagatga atacaagcat tttttaaagg ccgaatcaat
     3121 cccaacccct attcatacgc cgaatagtcg gtatgtgaat aacaagaaag tccaggaaca
     3181 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aactaagtcc
     3241 actgcagcag gccatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     3301 atatacctat gaagaaacaa cgatcttgac gcaagtccag cagcttcagt taaaggcaat
     3361 cggaaaagta ccgatagatc taggttggaa gaagttattt ggcaaagaga acgacggaaa
     3421 agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtaga
     3481 tctacaagtc cttgaaaaag aaaccaagcc accacagcct tataccgaag gtacgttgat
     3541 cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa gaagcgcaat ccatattgaa
     3601 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     3661 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     3721 ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg ccaaatggga
     3781 aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc tagccaatat
     3841 ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat ccactgattt
     3901 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     3961 aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcatg
     4021 tacgtctgaa gcctgtgatt tcaaactatg gacaacgatt gccaaaaaga aacttacagc
     4081 gacacagctg aaagagatca ttcaaaatag ccgaacaagc caaccagtga aagggttaaa
     4141 aggtcagaag ggttcgtttg aagcaacgat tgttttaaaa gaagacttca ctacaggctt
     4201 tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca ctacaaaata
     4261 atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg aaaatcaaaa
     4321 agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac tgcagcaatt
     4381 gaagaaccag cagcggattc aaaaagaaat cgaattagat cgataaattc ctttcttgtt
     4441 aatataaaac taacatgtta tagttgtgtt agttttatat taactttagg ggaagagtaa
     4501 tgaaagacaa aatccaatgt atttttgata aacataatgg caacctatcc ttgaaggatg
     4561 ctagaagtga aggattatcg cctatgacgt tagatcgatt agccgaaaag gataaaatag
     4621 aaaaggtagc accaggtttc tataccttaa aggatgcgtt cgtggatgaa ttgtatctcg
     4681 ctcagagtat ctattccaga gggatttttt ctcatgagac tgcgctcgat ctttttcaag
     4741 taggaacata tatccctaaa aagatcaata tgatgtttcc taaaggatac aatgtatcaa
     4801 aggagaaatt agatcagtat gccattcaac cgcattacac taataaagaa aattttgacc
     4861 tgggagtcac agaaacagaa agcttttacg gcaataaaat tttagtctat gatttagagc
     4921 gaacgttatg tgacatgtgg aatccaagat acaaagcaag cgtagaggta aaacaagaag
     4981 cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga agatacatgg
     5041 atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat tgagaggagt
     5101 aaagatgaat gacaccgaca caatttaaag agaaaacaag aaatttcgca gcagagaaac
     5161 atttaaaccc acaaattgtc caaagatatt atatgatgga aaaattatta gaatgtattg
     5221 ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatt ggatcaaaat
     5281 acgggttaga aaaccgttca accgttgata ttgatacgac ttatcgaaat tcgaaactaa
     5341 caaaagaaaa gttagaaact gttttttctg aattaacagc taatcctaca aaagatggaa
     5401 ttcgttttga attaaaggga ttaacagaga cgagggaagc cgattattat ccaggttttc
     5461 aagcaaagtt tattgcttat cttgaaaatg cacgtgtacc ttttaaatta gatattactt
     5521 caggtgattc tattattcca gcagcattga tgtatgaaca caaattaatg tttgaagata
     5581 aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa ttacatgcca
     5641 cgtttagttt tggaaaagat aattcgcgca tgaaagattt ttatgacctg tatatgattc
     5701 cgaaattaga acgtatcgat gagaaaaatt tgtataagag tgttcaagga acattcaagg
     5761 aaagaaataa tcaatcggtg tttaagcatt tgtatgatca ggaaatgcct caattggtga
     5821 aagatgaggg cttgagagaa aagtggaaaa gataccaaca agaaaactat tttgccaaag
     5881 acatttcttt cgaatctaca ctaacttccg ttgattccct gatgcaatca attgtagagc
     5941 aagagaaggc agaacgaaaa caaaatttta agagatcaca acaaatgaat caagaaatgg
     6001 agagataaag atgatcaata atgtaacttt agtagggcga ttaactaaag atccagattt
     6061 acgttatacc gcaagtggca cagcagtggc gactttttcc ctagccgtta atcgtaattt
     6121 cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt ggcgcaaacc
     6181 agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggcgttg tcggacgaat
     6241 tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc
     6301 cgataacttc caactattag aaagtaaagc agcgacagaa agccgtatgc acgctgatca
     6361 atcgtccagc agtccaagcc ctaccacctt tgaacaacgg gatacagcga cgcctaacaa
     6421 caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata ttagcgatga
     6481 tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt tatgttataa
     6541 tttagacaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg cgggctagtg
     6601 gctttctttt acgtttctat tataagaatt aacgggtagg cttactgtct ttcttggcaa
     6661 atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg attgcaaaaa
     6721 caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt aggccggggt
     6781 agcagacagc aagactatta tagcataatt gaaatatcca aatgattgga tttttgctat
     6841 aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
     6901 ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga atcgattaat
     6961 tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga tggaaaatac
     7021 aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc tagcatgtat
     7081 gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaataaa
//
LOCUS       DAZUKD010000133         6841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000133 DAZUKD010000000
VERSION     DAZUKD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.136"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..740)
                     /locus_tag="ACG8N9_002487"
     CDS             complement(96..740)
                     /locus_tag="ACG8N9_002487"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4898035.1"
                     /translation="MTERKNYSGGKIMKSNIKIIAGDKMKEKENLVWYFNRNPHMLIT
                     GRTATGKTYLLKRMADYLSQVGIVSASFDKTEFEEILSSSRKEMEDRFEYIKENRFKM
                     FDVSYSDLDMKPYFVLIDELDSRLLLLEKENPLAYNYLLLIIKDIVLKGRVVGVFFIA
                     VRQGKQPLLESIEDNMLTRIFLGDSKGNGTICFDGNTKKFQTELVTEDFFSVDC"
     gene            complement(792..2666)
                     /locus_tag="ACG8N9_002488"
     CDS             complement(792..2666)
                     /locus_tag="ACG8N9_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898036.1"
                     /translation="MNNTYVSDLTSLVGYQFTPSYIRTGNRWGTIVKLVNTYGMNRNY
                     EFGWMVKAIPEVKVEGVKAYLIEADKPVSQKDQQEIMHENVGGSTNSYENNLNDKNSD
                     AEINKTVMRIIDLNTASLLDSTANKIIDWKLEILLVSDSPNKISEQLIKLQRLYDEKM
                     YGLKLMSFGGDQRDLFDRLLSEPVGSVYEYTSMSSDFAGNDHAIRKGLDDADGWAIGT
                     MSTSYASGVAFMAMDRSFKGKVLVASHKDATIKGYSEEISASSLWGQLIANNAMANHK
                     RTFHIVLNKFDYFGDPTGETFVAKPSLNSVIEKINLAEEGLNSLELFGDRKDIGDLYR
                     RNLDKLTQIFYLLADRELNPATRVLLKKELGNFYISQKLWDINYEERSKLLVNSSDTM
                     PVLGDFISYLTNAETKAKKSGTEKEIDRIIVLKDTLRTALDSFSKMFNQRTTLKEIGK
                     SDKLQFYYNLSDLRTEINVMEAQFLNTFDFITSQAKKGDIVMIHGFDRLSVETVGLIK
                     KRLTMLEEDGVRLAFLFDTIGSGESKDGIETADIFNTDGKIYQSFESDFNYTIIGKMT
                     SEELMKYEKKVAPARKGEDDQKLSVDLRQYLTSGRSNKFQIRRPADLTNNFVIGEFII
                     "
     gene            complement(2768..3634)
                     /locus_tag="ACG8N9_002489"
     CDS             complement(2768..3634)
                     /locus_tag="ACG8N9_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898037.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            complement(3624..3854)
                     /locus_tag="ACG8N9_002490"
     CDS             complement(3624..3854)
                     /locus_tag="ACG8N9_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4898038.1"
                     /translation="MREENIVYPVIENQSNTKSSQLLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            complement(3866..6031)
                     /locus_tag="ACG8N9_002491"
     CDS             complement(3866..6031)
                     /locus_tag="ACG8N9_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898039.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGAKTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            complement(6031..6360)
                     /locus_tag="ACG8N9_002492"
     CDS             complement(6031..6360)
                     /locus_tag="ACG8N9_002492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898040.1"
                     /translation="MEDMPICSIYFLLKNYTRSVVFEVREDNRCKMDREKMLVHANEV
                     LTSGFKGTDLAKIMNMNVNQFYDYRNGNKKIEKARLETLIKFEKAYLYVLDKQKRTID
                     RKKGVLQ"
     gene            complement(6379..6717)
                     /locus_tag="ACG8N9_002493"
     CDS             complement(6379..6717)
                     /locus_tag="ACG8N9_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898041.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
BASE COUNT         1894 a         1402 c          990 g         2555 t
ORIGIN      
        1 tcttcatgtt gaattaggat tacttctgtg tctttaccta gaaatattcg atctaaaatt
       61 tttatgtttg gtttcattat ttaacctccg ctatactaac agtctacaga aaagaaatct
      121 tctgttacta gctctgtttg gaatttcttt gtatttccat caaaacaaat cgttccattt
      181 cctttggaat cccctaaaaa aatacgtgtt aacatattat cttctataga ttctaacaat
      241 ggttgctttc cttgacgaac ggcaataaaa aaaacaccta ctactcgtcc ttttaacaca
      301 atatccttta ttattagtaa taggtaatta taagccaatg gattttcttt ttctaagagt
      361 aacaaccttg aatctagctc atctatcaga acaaaataag gcttcatgtc taaatcagag
      421 taacttacgt caaacatttt aaaacgattt tctttaatat actcaaaccg atcttccatt
      481 tcctttcgag acgaagataa aatttcttca aattcagttt tgtcaaaaga agcagaaaca
      541 ataccaactt gcgataaata gtcagccatt cttttcaaca gataagtttt tcctgtagct
      601 gttcttcctg taatcagcat atgcggattt ctgttaaaat accaaactaa attttctttt
      661 tccttcattt tatcacctgc tattattttt atatttgatt tcattatttt accgccacta
      721 taattctttc tctcagtcat gtatataaat attgtttctt atccttaaac attcaaaaac
      781 aaggataaga cttaaataat aaactctcca ataacaaaat tattagtcaa atccgctggt
      841 cttctgattt gaaatttatt gcttcgacct gatgtcaaat actgtctcaa atccactgat
      901 agtttttgat catcttctcc ttttcttgca ggagcaacct tcttttcata cttcatcaat
      961 tcttcactgg tcattttccc gatgatggtg tagttaaaat cactttcaaa actttgataa
     1021 atttttccat ctgtattgaa aatatcagct gtttcgatac cgtctttaga ttctcctgat
     1081 ccgatagtat caaagagaaa cgcaagccgg actccatctt cttcaagcat tgttagtcgc
     1141 tttttaatta atccaacggt ttcaactgat aatctatcaa acccatggat cataacgata
     1201 tcgccttttt tagcttgtga cgtgatgaag tcaaaggtat ttaaaaattg ggcttccatg
     1261 acattgattt ctgttcttaa atcgctcaaa ttgtagtaaa actgcaattt gtcgctcttg
     1321 ccaatttctt tcaatgtagt tctctgattg aacatcttag agaagctatc caatgctgtt
     1381 cgcaatgtat cttttaatac aataatccga tcaatttctt tttctgtacc ggattttttc
     1441 gcttttgtct cagcatttgt taaatagctg atgaaatccc caagaaccgg cattgtatca
     1501 ctgctgttta ctaacagttt cgaccgctct tcatagttaa tatcccaaag cttctgacta
     1561 atataaaagt tccccaactc tttcttcagc aatactcttg ttgcaggatt taattctctg
     1621 tccgctaaca gatagaaaat ctgagttagt ttatcaagat tacgtctata taaatctcca
     1681 atatcctttc gatcaccaaa aagctctaaa gaattaagtc cttcttcagc tagattaatc
     1741 ttctctatca cactatttaa agatggctta gcaacaaatg tttcccctgt cggatcacca
     1801 aagtagtcaa atttatttaa tacgatgtga aacgtccgtt tatgatttgc cattgcgtta
     1861 ttagcaatca attgccccca aagtgatgat gctgagattt cttcgctata gcctttgatt
     1921 gttgcatctt tgtgggatgc tactaagact ttacccttaa aagaacgatc cattgccata
     1981 aacgctacac ctgaagcgta gcttgtagac atcgttccga tagcccatcc gtctgcatca
     2041 tctaagcctt tacggatagc gtgatcattt cctgcaaaat cagaactcat agatgtgtat
     2101 tcgtaaacac taccgactgg ctcacttaaa agcctatcaa acagatcacg ttgatcgcca
     2161 ccaaaagaca taagttttaa tccatacatt ttttcatcat acaggcgttg cagtttgatc
     2221 aactgttcgc tgatcttgtt aggagaatcg gatactaaga ggatttctaa cttccaatca
     2281 ataattttgt ttgccgtact gtcaagcaga gatgctgtat tcaaatcaat gattcgcata
     2341 acagttttat tgatttctgc atctgaattt ttatcgttca agttattttc ataactgttc
     2401 gttgatccac cgacattttc gtgcatgatt tcttgctgat ctttttgcga aactggctta
     2461 tccgcttcga ttaagtaagc ttttactcct tcaaccttca cttcaggaat agctttaacc
     2521 atccatccaa attcataatt tctgttcatt ccatacgtat tcactaattt aacaatcgtt
     2581 ccccaccgat tacctgtcct aatgtaagat ggtgtaaatt gataaccaac caatgatgtt
     2641 aaatcactga cataagtatt attcaccgct ttagaactat cagcattttg ctcttttttg
     2701 tgtttctttt ttctgaaatt aagtagcttc gatctactca ttagttccat taaatttctc
     2761 acctctatta tttcagtgca taaatttctg ataatatttc tttggtttgt ttagctgaca
     2821 actgagtaac gctatagtaa agccctctac tcgtgtaatt ttctaaacgt tctacgcctt
     2881 catccaatac catctgagat tgtgacttca aaataagata ttggatcacc gtagttttga
     2941 ccccatcaat atattcagat acaaacctct cttgttgatc tactatgtca aacacagctt
     3001 tgttgctctt tgcatttctt ttttgtactt tcatgttccg tatttgacgt tccgtattct
     3061 gaggttgtga tgaagtaatt ttgatttcag ccaaggaacg atcccttgca ttttggtatt
     3121 gaattgattt gccttcttgc gctatgattt cttctgggaa ggcggttaac gaggtagcac
     3181 catccaaatt aaacattagt gctacactac catctcgata cataatcgtt ccatcttgtc
     3241 taactttcaa aatctccgtt cctcgaaatg gtttcttttt ccccatcgtt actcttgctg
     3301 tgttaactac tgatttaaac agtctaatag agcctgagat cagtgatccg ctagatggat
     3361 caataaacat atactgacta atcagtataa gaaaaccaat tatcatcaat agcgttggaa
     3421 taaaaataaa tctcaattta atcgtatcgg aaaccaaata aacgattgaa ccaagaacaa
     3481 caactgcaac aacaatagct gaaacaatta gtctttttgc tgtaacagaa taggtgctgt
     3541 tgacatgttt ttcaagccaa tcaactctga ctattgtttc agaaggtttt tgatgcaaat
     3601 tatgtttaaa tccatagttt gagttatctg ccatcagaaa tacttccttt cactttcagc
     3661 ttacgataaa caccaacagt tagcgccgtt gcaacaataa gcaagccaat taatgatatt
     3721 gacttgctca ctgtttcatt cgtttgtggt agttcattaa ccggataaac gacttgcact
     3781 tcttgaagaa gttgagaaga ttttgtattt gattgatttt caattactgg ataaacaata
     3841 ttttcttctc gcattttagc cctccttaat tcttttcagt taagtgagct tgtattttgt
     3901 attgattccc atctggagct ttcacaactg ccttatcacc gtcaatggaa agtacttcag
     3961 ccttagtagt tttcccatca atactgatga caacatcttc tccgacttta aattctgtcg
     4021 ttggtgttgc ttcatagttg ttgtctgttg ttgaagtaga agctgtcttt gctcctagtg
     4081 aattttccac tcttgaaatt gaggtttctg tttgagcttg ggcttcttgg tgattatgac
     4141 taatgaagta ggatgagccc aaaatcgttc cacaagccat tgctgataca gcgatgatag
     4201 tcgccatctt tttgcttgta aaatcacgtt tagactcagt ttgattttct gtagtaacta
     4261 ctagtgactt tgttttttcc gccatttgtg ataggtcgtt tcttgcacga gataaatctt
     4321 ctcgtaaagc ttcactttgt tcagtcaatt tttggctatt taatttatag ccatctgcta
     4381 aatccttgaa ctcttttact cgatttttat aatcttcaat aatgttttgc tgtgcaacaa
     4441 gtttggattc taactcattc gtatctttag ctactagctt ttcaacttct tctttgttcg
     4501 caaaatattt gacaaccaca tctgttgttt tctcttttgt atcgtggaac tcagcttcat
     4561 tttctgcctt tgttttttcg acaatctcta acgttttttc ttgcacttct gttaaagatt
     4621 tttcaatttc cggtttcaat gaattgatga tccgttccac cacaacttct tttgcttctt
     4681 gattttgttt caaagcatcg tttttaatag attcaattgc ttcttggtat tgttgatcaa
     4741 gactttcttc agttgctttt ctaaattcat cttcttgttg tttaacttct ggcagatgtt
     4801 cagcatcgta ccgttgctct agtttcttta gttctgcttc caaataagcc tgtttcgcca
     4861 tatcataatc tttacgaatg atactggatt tttccatcac taaatcatca attttagatg
     4921 cttctgcttc gtagctttct ttagcgaccg atagcgtatt tgtaaactga ttttgaccac
     4981 ttagtgatgt tgcttcctct atccgcaaaa gctcatccgc taacatttgc tttcctttga
     5041 gtagcaattg ctctttcgtt ttatttaatt cactgttgat tttattatta gcaatagaga
     5101 tacgagcgtt catgctctct ttagctaaaa acagcaatgg ttttaactct gcaatggatc
     5161 gcatctcttc gttcaaatca acaggatcac taacctcatc taaaactaca ggttgaaatt
     5221 tattggctat gatctgttca aaatcttttt ctaacaaatc agcatctaaa tttttcggta
     5281 catcactttc agatacgatt ttttcagctt cataaacata attttcttta tcactctggg
     5341 attcgggttt ttcatcaatt tttggttgtt ggtaggtttc tgtttgtgtt tcttctatgc
     5401 ttgcttcttg gtttcctgct tcagttgctt cgtcattatc ctcaaaaaga gcttcttctt
     5461 catctggtac ggcttctgtt tcggcttctg ctacagcttc aaattcaacc gcatcagaag
     5521 agttgaaggt ctcagcaatt ctttctaagc ctttagtagc tacctctaat tgatctactg
     5581 cactgtagaa gtctgtagct tcttcaactt ctctttcaaa catattagga tcatcaggaa
     5641 taactaataa ttgaccatta ctatccataa agcggacacc agctaatagc ttttctttga
     5701 atttttccgg taaatcaaca acataattca gacttgattt taataaaaca acgtactttt
     5761 cggaatctag aatcgcatct tcctcatccg gaaattcctc actgctgagc ttttgtacgt
     5821 cctcaatcac cacgtaattc ttctttggta aatctttggt cagcaacgca tcgaattctt
     5881 tccatacctt tgctttagct ttttttgcct tcgcttcttt ttcttctttt tggtacactt
     5941 cgagtaattg tggcttcaaa gagagtacaa ctacgggaac tgtttctgct acataattaa
     6001 tagattcggt aatatctttg aattttgcca ttattgtaaa actccttttt ttcggtcaat
     6061 agttcttttc tgtttgtcta acacgtatag atacgctttt tcaaatttga tgagcgtttc
     6121 aagtctggct ttttcaatct ttttattgcc gtttcgataa tcatagaact ggtttacgtt
     6181 catattcatt atcttcgcca aatcggtacc tttaaaaccg ctcgtcaata cttcgtttgc
     6241 gtgaactaac atcttttctc tgtccatctt acatctattg tcttccctca cttcaaaaac
     6301 tacgcttcga gtgtagtttt ttagcaaaaa gtaaattgaa caaatcggca tatcctccaa
     6361 atttgctcaa tttacctttt aacctaattg ttcttctttt ggaaactttg taactagata
     6421 aataccaacc acaggaataa gagctgagat catattagct ttttggttta agtcattacc
     6481 tgttttagta cccatcgtta acaacataac agttgtaatt acagggataa taaaaatagc
     6541 aacaacggct ccaacatatt ttaagccttc tccccatttt gcatcaccga aatgtttcat
     6601 agcagtgaat cctggtttca atgcaaaaaa tgtagccatt aaatatagaa tagcttgaac
     6661 caacgttttc atttctggcg tcattccgga aaccaaattc ttaatagaat caaacatatt
     6721 ataaatcctt cctttcgatt atttttcaca agataattat atcatcgcaa acttttctct
     6781 gttcacttgt caatgatcgg aatacagaaa agacgcttcg atatttacat ctttatgtta
     6841 t
//
LOCUS       DAZUKD010000134         6815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000134 DAZUKD010000000
VERSION     DAZUKD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.137"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..2233
                     /locus_tag="ACG8N9_002494"
     CDS             191..2233
                     /locus_tag="ACG8N9_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4898042.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2244..2864
                     /locus_tag="ACG8N9_002495"
     CDS             2244..2864
                     /locus_tag="ACG8N9_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4898043.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2876..3334
                     /locus_tag="ACG8N9_002496"
     CDS             2876..3334
                     /locus_tag="ACG8N9_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4898044.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3353..3631
                     /locus_tag="ACG8N9_002497"
     CDS             3353..3631
                     /locus_tag="ACG8N9_002497"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4898045.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3656..5023
                     /locus_tag="ACG8N9_002498"
     CDS             3656..5023
                     /locus_tag="ACG8N9_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4898046.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5038..6183
                     /gene="dgoD"
                     /locus_tag="ACG8N9_002499"
     CDS             5038..6183
                     /gene="dgoD"
                     /locus_tag="ACG8N9_002499"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFS4898047.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6210..6701
                     /locus_tag="ACG8N9_002500"
     CDS             6210..6701
                     /locus_tag="ACG8N9_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898048.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
BASE COUNT         2149 a         1111 c         1487 g         2068 t
ORIGIN      
        1 aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca tttgatataa
       61 cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag tgtgacaaaa
      121 aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt ttgtcaggag
      181 gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta aaaaccctga
      241 aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa tcaattacgc
      301 gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc agagaagtca
      361 ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc ctgattacga
      421 cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag cattgatagt
      481 gatgactctg atcgctaata tcgattacat cagtctggat catttggtag atgcggttga
      541 tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca tcagaaatta
      601 tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt cggaacaccg
      661 tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc gagaaatgat
      721 tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga tccataacat
      781 ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc agacatcagc
      841 gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt ttcccgatga
      901 tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa caggttggag
      961 tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca atatttttga
     1021 gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga tcagccaaat
     1081 ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag actttgaacg
     1141 taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact tgagtttagg
     1201 agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga ttgatttgat
     1261 gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa atgatgaatt
     1321 agatctttta tcctattact ttggctatca attaagtaat cataataaat tgaataagca
     1381 acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt tagtacgagc
     1441 caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg tgcgtgattt
     1501 ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa aaagttctat
     1561 gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt tacgatatcg
     1621 agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat tgttaacaat
     1681 cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac tgcagtactt
     1741 tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc cttcattgac
     1801 ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt gggaacgagc
     1861 agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc gttttatcgc
     1921 tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa tgaagacttg
     1981 cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca ttttagtttg
     2041 taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc cgctttcatt
     2101 ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg ccaaagatga
     2161 ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc aactattaag
     2221 acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg acagtgatca
     2281 tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag gaatttgacc
     2341 atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat atacatgaac
     2401 taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg acattagaac
     2461 aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat atgttcacaa
     2521 aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc gcaatgactc
     2581 cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta tttcctgcag
     2641 cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag ttaccactaa
     2701 tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa ggtgctagct
     2761 atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta aatgttaaaa
     2821 acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga ggactttgga
     2881 actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag atagtgaagt
     2941 cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg aatatattca
     3001 agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac caccaggaat
     3061 cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa ttgcgactct
     3121 taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac caatccaaat
     3181 tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac aaaaaattgt
     3241 tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca atgatactga
     3301 attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca taatgaaacg
     3361 aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa caaaagttag
     3421 agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat tgatggaagt
     3481 tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg atggtcaaac
     3541 aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg ccgatcagac
     3601 aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg aaaaaatgaa
     3661 aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta tgttgccagt
     3721 catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt ttaaatcagg
     3781 tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta tgacgaccaa
     3841 tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag atgctttgga
     3901 tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc caatcttgat
     3961 ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac atacgctgga
     4021 tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt attttgtgac
     4081 taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga cttttaagat
     4141 ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg tttctttacc
     4201 aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc tgctggatcg
     4261 tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat atttgggatt
     4321 ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg ttttagccgg
     4381 ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt tggttttgat
     4441 tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg ctgcacaaaa
     4501 atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg ctgcagttgt
     4561 tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa cgattgcatt
     4621 agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg ttgttccttt
     4681 ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata tcattattgg
     4741 cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa tgctgacagc
     4801 aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct catcatttgc
     4861 tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca aaccaatcat
     4921 cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg atggtatcaa
     4981 aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg gtttaaattg
     5041 aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt taagatctcg
     5101 actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac agaaacggtt
     5161 gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt tgaaatcgag
     5221 cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa tggaaccatc
     5281 gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa tgttccggtt
     5341 tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg gattggtgga
     5401 gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg ctttacggcc
     5461 gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa agttgatgaa
     5521 gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga tattggtgtt
     5581 gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc tttggagcca
     5641 ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca ctttaaagaa
     5701 gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac tcgttggcaa
     5761 ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt ggctctttgt
     5821 gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga catggctgtt
     5881 gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt agactcatgt
     5941 acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa aggttttgat
     6001 ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta cgtagatcta
     6061 ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga aattgcacaa
     6121 gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat cgctgagtgg
     6181 tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc acagtttttt
     6241 taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg tttgctcaga
     6301 gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg ttgattttct
     6361 ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc attttgatca
     6421 atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga gtattgaacg
     6481 gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat aaatcattta
     6541 tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa gatcaaactg
     6601 aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta gatcaaagtt
     6661 cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac cgaaattatg
     6721 atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg aagtatacaa
     6781 tgtatattaa gttttggaaa gaaatatatt ggtac
//
LOCUS       DAZUKD010000135         6788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000135 DAZUKD010000000
VERSION     DAZUKD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.138"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(348..1109)
                     /locus_tag="ACG8N9_002501"
     CDS             complement(348..1109)
                     /locus_tag="ACG8N9_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4898049.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            1209..2930
                     /locus_tag="ACG8N9_002502"
     CDS             1209..2930
                     /locus_tag="ACG8N9_002502"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898050.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            2945..4729
                     /locus_tag="ACG8N9_002503"
     CDS             2945..4729
                     /locus_tag="ACG8N9_002503"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898051.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(5110..6663)
                     /locus_tag="ACG8N9_002504"
     CDS             complement(5110..6663)
                     /locus_tag="ACG8N9_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4898052.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
BASE COUNT         2100 a         1328 c         1205 g         2155 t
ORIGIN      
        1 ttttaagtgt aatgctttta tgcacctatt taatttatca ttctcatctg atacaatctc
       61 actcataaca atatgaattt ttcttctcat tagtctcagt tcaccatgaa ttatacataa
      121 cacccaatac aattattttt ccatactttt ttaagaggac aattacatcg tatatatcgc
      181 taagaattgc aaccattcca tttttaaaga catatttcct ctaaaaaact aaaattctat
      241 ctatccacga ctaataatcc cccacattaa acccgtaatc gaaccagaaa atttttcttt
      301 ataactaaaa caaccatcat tttcgagtta tcttaaacac atatttccta ttttttcatt
      361 tctatccatg catgataata ttcagttctg atattgtttt catccgtatt cgtcaataga
      421 tactggccga tcacttctcc tgttttccat cctttttcat acattctttc tctgatttct
      481 aaaatattat tacgttccgc atggttcatc tcggatttca ataagaatcg acgataaaca
      541 gccgtatctt ttttctccca aatcacgtct tgagtaaata aatgtacttc actagggtcg
      601 ataaaaaatc catacatcgt ctgtgaactg gaagctggca tactcatcat cccaaagctt
      661 gaatagttag gtagaaattt tttgatttcc acaatatctt caaactccgt tgagatcata
      721 cagtcaaaag ggatctcttc tacaggaaat tcttcctcgt ttgtatcgat catcaatttc
      781 aactgttttt ttcgctccct tatctctttt tgtttttgtt tcatcaccac cagttcttta
      841 tggattcttc tttctgtttc atcaagtgtc tcatataact ccgtcaatgt ttttccatat
      901 aaattcttca tctgtttgat cggtatatct aattttctat aaaaaacgat atcataaagc
      961 ataaaaatat catcgatatc aaacaatcga taatcattct cttcttgtct acttgaacgg
     1021 attaatcctt gttcatccca atagcggatc gttgattttg gcaaatcaaa tagttccgat
     1081 acttggccaa tcgtatacat ttttttcatt ttttctcacc tctttttcag tttattcttt
     1141 tttctattga ccttcaagtt acttgaaggt atagaatcga aaagtatcag gaaaaaagga
     1201 ggaataatat gtctatctta aaaacattct tacagaaaaa caaaggactc gctctcctta
     1261 cctttgtgat gatctgttta caaatcgccg gtacattagg tgtaccaaaa ttagtagcaa
     1321 agctgatcga cacaggtata gcaagtggcg aagcctcagt tataaaaaaa attggagttg
     1381 aaatgctgct tgtagcgttt ctaggaacaa tcgctgcagt tgcctctagt tacttttcag
     1441 cattggcttc cgctaggttt ggattccaaa cgcgggagaa atatttccgt aaattccaac
     1501 agctgtcaat gaaggatatc agtcgtttta gttctggatc tcttttgaca agaatgacga
     1561 atgatgtcga taatgtccag caaatgattg ttttattttg ccaaatgatc ttaccagcac
     1621 cggttatttg cgttttcacc attctcatga tgtttcgtta ttctctatta ctaacatggg
     1681 ttacattgat ctctgttgtt ttttatgtat ggattgttta tcgactgatg aaacgagggg
     1741 cacctttatc tttgagtatc cagccaaaaa tggatcggat caccatcaca ttgagagaat
     1801 tctttactgg gattaatatg atccgtgcct ttgataatca atcatatgaa gaaaagcgaa
     1861 cgaataagag ctttgctgac tatgctaatc aaatgatcaa agtgaaccga atatttgctt
     1921 gggtcactcc aattgctttt ttgctcatgg gaatcgttta tgcttctatt ttgtggtttg
     1981 gcggaaatct tgtagcagaa ggagcattac aaatcggtgt cgtgactgct gtagtcgaat
     2041 actctatgct gacacttgct tacttgatga tcgcagctat ggtattggtc attatcccta
     2101 aatcgtttgc ctcattacga cggatagaag aaatactgca agcagagatc gaaattgagg
     2161 aagagacgat caaaacagta gctgaaccaa cctttgatca gacaaatgca gctgcttttg
     2221 aacatgtcac atttaaatat actgaaatct ctgatccagt tctagaagat attgatttca
     2281 caatacctaa aggaaaaaca acagcgatcg ttggcgggac cggatctgga aaaagtagtt
     2341 tggcgaaact tctcttgaga cttagtgacc ctacgatagg aaaagtaacc cttggtggta
     2401 ttcctcttac tcaaatgact caagaacaga tccgctccca catcagttat gtccctcaga
     2461 aggctttctt attcagcggg acaattttaa gcaacttgca gatgggaaat gaatatgcct
     2521 caacagaaga gttgtctaaa gcgatcaaga tcgctcaatt agaagaacta gtggacaccc
     2581 ttcctgacgg attggacagc tttgttgctc aaggcggttc taattattca gggggccaga
     2641 aacagcggat atgtattgct cgagcgttga tcaaaccagc agagatctat gtattcgatg
     2701 atagtttctc cgctttagac tataaaacag atgcagcatt aagagcagcc cttcataaac
     2761 acatgtcgga gaaaactctc gtgattgttg ctcaacgcct cagtacaatc caacaagcag
     2821 ataacatcat tgttttagat gaaggaaaaa tcgttggaca aggtactcat gaagaactac
     2881 tgtctaattg ccaaacttat caggagtttg caaaatcaca aggaatatag aaagaaggga
     2941 tacgatggaa aagaaaaaaa cgttgaacac gcgtgctttg atacgttttt ggaaaatgat
     3001 caaacctgaa cataaatatt tttacggatt gtttctatgc agtttattcg ggaacctatt
     3061 agtcgtagcc atgcccttga tcatgggaat cgggatcgat gatctcttat cagaaataag
     3121 aaaaacaggt attggacagc tttctttcag tgatatcaaa caagcattgt tgttccctgt
     3181 cttattgctg atcggctgtt cagtcttaag cagcatcact tcatttattc aggaacggac
     3241 aatggcagcg ttaagcgaaa agatcacatt gagggtgcga aaagaaatca caaaaaaatt
     3301 caaaacatta ccgatgtcgt tttttgacaa tcatcaagta ggtgacatca ttagtcggac
     3361 aactaccggt ctcaatcaac tttctcaagt gttattgaca ggtatcaatc aattttttac
     3421 ttctgtcgta acaatagctg ctgctggtct gatgctgttt tatatcgaaa gcaagcttac
     3481 tttgctcgtc ttattgctga tcgttggcag ttcaattgtt acaactaaga ttgcgaacaa
     3541 aaacaaacag atttctgaac agaatcagta tgaactgggt gttttaaata atcgaatgga
     3601 agaaatctta gctggtaatc tagtggtcaa aacattcaat cagcaagctc atgcagaagc
     3661 tgctatacac gaagtaaata aaaagcaata caacgctttt aaacgtgctc aatttatgaa
     3721 ctttgctatc tatccggcca ttcgtttcat taatcaactc gccttcatca taagtgcggt
     3781 attcggtgct atgcttgttc tatcaggtgg tgtgacgatc ggcttcttac aagcttatct
     3841 tcagtacatc aatcagattt ctgaaccaat ttctaccgct tcttatgtga tcaactcgat
     3901 ccaatccgca atggcagcaa tcgataggat atttgagata ttagatgaaa cagatgaaat
     3961 tccagacctt ccagatgcaa gacttttaga acaaccgaaa ggaaaaatag ctttcaaaaa
     4021 tgtacagttt ggttacacat cagataaaat attgatgaat caagtcgatt tttcagtaaa
     4081 gccaaaacaa acggtagcga tcgttggtcc aactggtgca ggaaaaacga ctttagtcaa
     4141 tctgctgatg cgtttttatg aactgaataa aggaaagatc gaattcgacg gtcttgatat
     4201 taccaagctt tcacgtggag aactacgtcg ttcttttggg atggttttac aaaatacctg
     4261 gctgtttgaa ggaacggtag ctgataatat cgcctacggg aaaatggatg ccacaagaga
     4321 acaaattatc gaagcggctg aaatcgcaca atgtgatcac tttattcgaa cactgccaga
     4381 cggatatgat acgatcattt ctagcgaaaa cggcacgtta tctcaaggac agcaacaatt
     4441 attgacaatt gctcgagtca ttctagcgaa tcctcctgtc gttattttag atgaagcaac
     4501 ttctagcgta gatacacgaa cagaagcatt gattcagcaa gcaatggaag ctgtgacaaa
     4561 agataggaca agttttgtga ttgcacaccg tttgtcaacg attgaaaatg cagacatgat
     4621 ccttgtaatg aagaatggcg atatcgtcga aaaaggaaca cattctgaac tactccaatc
     4681 accaacgctt tatgccagtc tttataatag tcaatttcag atagcgtaac aaacattatc
     4741 taaaaagcta tcgatcatgt atgattagtc catgatcgat agctttttta aatgtactag
     4801 tggtgccact agaaacacaa agaataatac taatgatact ttttggatag ctccttcagt
     4861 ttcgttcggt aagtagtgac aaaagacttg tcacaatccc tttttacgaa taaacggtgt
     4921 tcaagagaca acctatcggc aataagacac gatcttgtaa aatacgagaa gctatacttc
     4981 aaaatcgctt cttgttgctc ttgtcaactc cgaatcacga gttacactat tcaacaaata
     5041 tgagaagctg ttttttgaat ttgtttcttg tgactcggtg ctgaacgtca ggctcacaat
     5101 ctctcttctt taatctcctt gttctacttg cgatagttgc tgaacgaatt gccatgtttc
     5161 cccgttatca gttgaacgga agaggcaggc aaacattttc cctgcatcga ttggtccttt
     5221 tttcaacaaa taaaattttg tctctaacgt attcgcgctt atttgagtaa cttcatttgg
     5281 tgattgaaaa agatctatcc cccctgtctg atagttttcc ggaatcgaca gaactgattc
     5341 tttgaatgag cgtcctccgt tgtttgtata aaacaaactt tcacgtgtcg aaacaaaacc
     5401 taaagaagtt gtaacgaagc tgacattttg tactggctga ttgatcattg tacttccact
     5461 ttttatccaa gtgattccgc catccatagt agtgtaaatg tacaaagttt ctgcagacat
     5521 cccactggct gatgtacaga taaatagacc atcgttgcca taaaataccg cttttcgata
     5581 acgtatcggc tgaactgatt gggtgataat agacgtttgc catgttttac cattatctct
     5641 tgaagtcagc agacgtacat catccccaga agaatagata aaccatgaga aatcggcgga
     5701 aatatcatat gtgttgtcca tccaataacc ttcaggaatc agccccgttg tcagggtgta
     5761 ttcgccaaaa cgcaaccatt gcgaatcaat cggcacaaat acccaattag caccactatc
     5821 tgttgtcata tagagagagt gcgtctctgt atcaagtttg agatcattta tcttttgtgt
     5881 tgtcaaattt accttttgct taggatcagc taacaaccct tcatttgtct tttgtatatg
     5941 cgtatcgtta gttgagttca ttgtcatgtg agaagcagca tttatatgag aagacatttt
     6001 tggctgagtg tatctcccat aatagattgt tccagctaaa agaatgagga aaaaaccagt
     6061 gactaatgta ctcatcttag ttgtcaggta tccaccaggc gaagactggc ttttttccca
     6121 tctgggttcg ttttctagta aaaaaatatg gaatgtgttc attccgaatt ttgatttgct
     6181 ttcgcctaag tagataggta aatcatcaag tgatctcagc aataactggc aatcactttt
     6241 ttgtatccga aaagcttgac tcgtcgaagt cttctcaaaa taaagatagt catcatcttg
     6301 agaagttggt ttattatgta aaaactgttg tgctctttga ctaagccatt gatcgaaatg
     6361 acgcgacaat tgatggtaac ggagccaaat aagtagaaga aaaacaataa gcacaacaat
     6421 tgttattccg ccaactcccc acgaaaagtg atacaaatta tttaattttt gcatcccttc
     6481 agctgaacgg actactgcac tcatatggcg atataggaac gatacagctc cgaaaaaaag
     6541 caaaccagag aaaaatacta agcttaaaag cttgaacatt attcttttcg ctcttttttt
     6601 cgctttctcg ataatttttt gtttagctga taatacttcg ataataaata gttcttgatc
     6661 catttgtctc tccttttctc atacttcatt cattatgcgt caaaatccat acaatcacta
     6721 gtcccactaa taaaaaattt atgaatttgg tatgtactct caatttcagg tagtttatac
     6781 atcaaaaa
//
LOCUS       DAZUKD010000136         6535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000136 DAZUKD010000000
VERSION     DAZUKD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.139"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..943
                     /locus_tag="ACG8N9_002505"
     CDS             89..943
                     /locus_tag="ACG8N9_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4898053.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            944..2281
                     /gene="xseA"
                     /locus_tag="ACG8N9_002506"
     CDS             944..2281
                     /gene="xseA"
                     /locus_tag="ACG8N9_002506"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4898054.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2286..2510
                     /locus_tag="ACG8N9_002507"
     CDS             2286..2510
                     /locus_tag="ACG8N9_002507"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4898055.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2512..3402
                     /locus_tag="ACG8N9_002508"
     CDS             2512..3402
                     /locus_tag="ACG8N9_002508"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4898056.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3411..4229
                     /locus_tag="ACG8N9_002509"
     CDS             3411..4229
                     /locus_tag="ACG8N9_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4898057.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4336..4785
                     /locus_tag="ACG8N9_002510"
     CDS             4336..4785
                     /locus_tag="ACG8N9_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4898058.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4803..6485
                     /gene="recN"
                     /locus_tag="ACG8N9_002511"
     CDS             4803..6485
                     /gene="recN"
                     /locus_tag="ACG8N9_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4898059.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT         2208 a         1102 c         1506 g         1719 t
ORIGIN      
        1 ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa
       61 aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc
      121 tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca
      181 tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa
      241 taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac
      301 gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca
      361 tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc
      421 tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc
      481 gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta
      541 ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa
      601 accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac
      661 cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg
      721 acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa
      781 tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc
      841 aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta
      901 tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt
      961 aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga
     1021 acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata
     1081 tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca
     1141 aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt
     1201 atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg
     1261 tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca
     1321 agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag
     1381 cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt
     1441 ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat
     1501 ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc
     1561 gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa
     1621 tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc
     1681 agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga
     1741 agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat
     1801 ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga
     1861 aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc
     1921 tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga
     1981 acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa
     2041 acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc
     2101 tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac
     2161 gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac
     2221 gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata
     2281 aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc
     2341 agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg
     2401 aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga
     2461 tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta
     2521 actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat
     2581 gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg
     2641 aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat
     2701 gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac
     2761 gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa
     2821 gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag
     2881 ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct
     2941 gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg
     3001 aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt
     3061 cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta
     3121 cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat
     3181 gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa
     3241 aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg
     3301 ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg
     3361 ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag
     3421 aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac
     3481 gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag
     3541 aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc
     3601 gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa
     3661 cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg
     3721 caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag
     3781 atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta
     3841 ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc
     3901 tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc
     3961 aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc
     4021 atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg
     4081 aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga
     4141 ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa
     4201 atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat
     4261 gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta
     4321 aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt
     4381 gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct
     4441 gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct
     4501 tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa
     4561 ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt
     4621 ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag
     4681 aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa
     4741 gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg
     4801 tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt
     4861 ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg
     4921 atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag
     4981 aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga
     5041 tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct
     5101 taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa
     5161 gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc
     5221 ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa
     5281 agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc
     5341 agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa
     5401 tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac
     5461 ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata
     5521 atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc
     5581 atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc
     5641 aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt
     5701 tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat
     5761 ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt
     5821 tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc
     5881 agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat
     5941 ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct
     6001 taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta
     6061 cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt
     6121 ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta
     6181 tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa
     6241 tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg
     6301 cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt
     6361 ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa
     6421 gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga
     6481 cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat atttt
//
LOCUS       DAZUKD010000137         6510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000137 DAZUKD010000000
VERSION     DAZUKD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.140"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(577..1089)
                     /locus_tag="ACG8N9_002512"
                     /pseudo
     CDS             complement(577..1089)
                     /locus_tag="ACG8N9_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(1215..1916)
                     /locus_tag="ACG8N9_002513"
     CDS             complement(1215..1916)
                     /locus_tag="ACG8N9_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4898060.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(2024..3467)
                     /locus_tag="ACG8N9_002514"
                     /pseudo
     CDS             complement(2024..3467)
                     /locus_tag="ACG8N9_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(3481..5346)
                     /locus_tag="ACG8N9_002515"
     CDS             complement(3481..5346)
                     /locus_tag="ACG8N9_002515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4898061.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(5467..6318)
                     /gene="licT"
                     /locus_tag="ACG8N9_002516"
     CDS             complement(5467..6318)
                     /gene="licT"
                     /locus_tag="ACG8N9_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4898062.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         1860 a         1466 c         1090 g         2094 t
ORIGIN      
        1 cttttattaa cgaaaaaatc attttgttct cgtgttatgt ttcactgata aatgatgttt
       61 ctctaaataa aaaagccgac gaactaaacg atcaaaattt gatcattttt gttcatcagc
      121 ttctcctaaa aatctttttg ttaacgataa atagctggat ttgtaaaaat atcacttgaa
      181 tcgtcactgc tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc ctggaactag
      241 tgtttgatat tgggctcgaa cgtgtactga tctctagcat gaagaataat ttcttttttt
      301 actgtataat aactttcttt cctttttgga atagtacagg aaagtttttt tgtttccttt
      361 ccttcgacat aaggtatacc agaccctttc gacaacgaaa ggttctcatt tttccttcat
      421 cgtcatttca aggagggtgc cgatgctctg gacagtttcg atcgctccta aaccaaaatc
      481 tgtatattcg attctttcta actctgattc tttcatacca ttcctgtctc aaggtacatc
      541 tattgtactt ttttcttgta atccattctg cctctcctaa ctaattgtca aatcatccca
      601 agatatgtct tcttcctctt tttcttcttg gatgatttct gcattcggat ctactggttt
      661 tttcaataat aaaatggtta ttcctgtgac aaaagaacca gctaacaatg caccaatgaa
      721 tgcccatggt ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc
      781 tgcgccccaa aacatgctta atccgccacc tactgtccca ccgactcccg ttgctatgac
      841 attgcgtgcc aagtcgttca atacgatagg gaaacatccc tctgtgatct gacagatccc
      901 cataggaaaa gcggtcttca atgtatcgat ttcttgacga ttgagtatat ttttacgaat
      961 gattttccct aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat
     1021 caatacagtg attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga
     1081 ataaatggta atccagaaac agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa
     1141 tatgagaagc tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca
     1201 acctcttttt ttatttatgt ttcataatca atatggtaag gatgcagctc cgtatggtag
     1261 accgcctccg aatattcaat catttcccca atttgcgtag acgatttccg tatccgtttg
     1321 atgccgatct gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa aaatactgcc
     1381 gagaaatcat cagaaaatga gtcaatagta tatccttttt tcgtcagcaa acgatacaaa
     1441 gattctttgc caaattcttc tctgctgaat tgcatcattt cttgtggaag ataatgaaca
     1501 aaataaatat aaggctgatt gttcaaataa tataagcgct taaaacaaac agcttgctca
     1561 ccgaaaaagg aaaacaaagg atcttccttc tttatatgga ccaattgcaa atccagcgtt
     1621 tctttccgga cttcatatcg ggagttctcc aagatttgcg taaagcttgc cgctttggat
     1681 aatcgattgt aaggacgatt gcttagaacc gtcgtcccac gaccactttt ctttgcgaca
     1741 tattcatctg atgccagcag ttcgattgcc tttcggacag tgattttgct gacttgaaac
     1801 agttcttcta attcactttc tgtcggcaac atcgaaccga ctggatattt tcctgagaaa
     1861 atatcttctt taattttatc tgcgacttcc aagtatagta cacgtgtcct tttcataacg
     1921 tcaccctttg ctttactatt actaaaagaa tagcacataa aaaataaaaa aagaacacaa
     1981 atcacatgtg agaagtgtgt tctttttcat tcagctgctt taattacaag tttacgccat
     2041 ttgaggcaat tacttcttta taccaatcaa aagatttttt ctttgatcga ttcagtgtcc
     2101 catttccttc atcgtctaga tccacataga taaagccgta acgtttactc atttctcctg
     2161 ttgatgcact gacaagatcg atacatcccc aaggcgtata gcccatcaaa tctacaccgt
     2221 ctgctactgc attagccatc gcttcgatat gacgacgtaa ataatcgatc cgataatcat
     2281 caataatcgt gccatcctct tccatatgat caatggctcc aagaccgttt tcaacaatga
     2341 ataatggttt ctggtaacga ttgtataatt cattcaatgc gattctcagc ccttctggat
     2401 cgatctgcca gccccattca cttgcctcaa gaaacggatt ttttactcct cccagcaagt
     2461 tcccttctga agttgctgct tgtggatcag tctcattgat tgccgtagac atataataac
     2521 taaatccgat gtagtccact ggatattctt ttagcaactg gaagtctttt tccgtttgct
     2581 ctaatttttc cggatcgact ccatattttt tatgggtacg cgctgtgtat gcagggtatt
     2641 ctccacgtac ttggacatcc gtgcagaaaa aattgaattc ttggttttgg atcattgttg
     2701 ctgcctgatt gactggattg gcatcgatgc tgtaagttgt tgcatagata atcatgcagc
     2761 cgatttggat atccgaacgc aattcgtggc cgatttttac ggctttgcta cttgcaacga
     2821 attgattatg ccatgcttgg aaaatatttt ggtactctcc tgctccattg ctttttacta
     2881 atccttgact taaagctgga aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat
     2941 atttgacttt actgctgtat ctctctaata ctgtctgagc aaatcgttca tagaagtcga
     3001 tcagttttcg atttttccag ccaccgtatt cttttgccaa atgaagaggc atttcgtaat
     3061 gagaaatcgt gatcacaggt tcaatatcat attttaagca ctcatcaatc agctgatcat
     3121 aaaattctaa tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc
     3181 aggcaatcga aaaacgataa catttgaatc ccatttccgc aaacaaggca agatcttctt
     3241 tgaaacgatc gtaatgatcg attcctcgat gattcgggta aatatatttt tcttgatcaa
     3301 tcgtccagtc aaattcttca ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag
     3361 catctgcaac ggatagtcct tttccgtctc gatcataagc cccttctaat tgatttggca
     3421 gcagttgctc caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc
     3481 ttattttaca attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag
     3541 tatatcggca aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc
     3601 tgctctaatt gcttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc
     3661 ttgtttgact aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg
     3721 aatcaaaatc tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat
     3781 agtcgtgatc tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat
     3841 accttttccc atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac
     3901 tttacccgtt aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt
     3961 cgcatgtttg ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt
     4021 acctacgaat gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga
     4081 ctctacacca tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct
     4141 ggagaaagct tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca
     4201 agcggcaatg aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat
     4261 tccaaataac gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt
     4321 tcgcaatgct acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg
     4381 catcaataca tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatcccca
     4441 atgcatgccg aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat
     4501 tggactaaat ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc
     4561 gattggtcct aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac
     4621 taaaatcatt tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg
     4681 aacccatacg gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat
     4741 cactgggatc ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg
     4801 atacacaagt gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc
     4861 tgctgtaaaa gcaagcatga caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa
     4921 tatttgataa gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa
     4981 tcctttcaat accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat
     5041 aatatcgatc agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc
     5101 aagaccttcc tcatccacga tcgattgata aacatctgct acattactgc caatcactac
     5161 ttgatactga ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg
     5221 cgtatctgct aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa
     5281 acttttgaca ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt
     5341 atccattgtt ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa
     5401 acagtcacaa acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa
     5461 caatccttat gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa
     5521 ataaatctgc tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat
     5581 ccgttcgaca caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc
     5641 atcttgctcg tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt
     5701 aatgatacgc tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat
     5761 gatactcagt atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt
     5821 tccttgttgg ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc
     5881 cacacccgtc ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg
     5941 ataaaatttt tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg
     6001 tgtgatcgca taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac
     6061 ttttagttta tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg
     6121 aatatcagcc atcagctgct ggaattggtt tttagcaaaa gaatctctta aaacaaattc
     6181 tttttggacc gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt
     6241 tcccatacaa acgacttctt ccccgaatcc atttttcgaa atgacgacat tgttattcaa
     6301 aattttatca attttcattt tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc
     6361 gcctactgtt tcttagctga attctggttt tgcctgccgt acagtaacaa tccatttctt
     6421 attcaaaatc atcataccat cgaattttta tttatgcaag ccttttcata taaaaaataa
     6481 aaaaactgga acacaagtct atctagaaaa
//
LOCUS       DAZUKD010000138         6446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000138 DAZUKD010000000
VERSION     DAZUKD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.141"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..898
                     /locus_tag="ACG8N9_002517"
     CDS             41..898
                     /locus_tag="ACG8N9_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4898063.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            918..1478
                     /locus_tag="ACG8N9_002518"
     CDS             918..1478
                     /locus_tag="ACG8N9_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFS4898064.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2533..3300
                     /locus_tag="ACG8N9_002519"
     CDS             2533..3300
                     /locus_tag="ACG8N9_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFS4898065.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3326..3871
                     /locus_tag="ACG8N9_002520"
     CDS             3326..3871
                     /locus_tag="ACG8N9_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898066.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3872..4438
                     /locus_tag="ACG8N9_002521"
     CDS             3872..4438
                     /locus_tag="ACG8N9_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898067.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            4453..4701
                     /locus_tag="ACG8N9_002522"
     CDS             4453..4701
                     /locus_tag="ACG8N9_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898068.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4721..4960
                     /locus_tag="ACG8N9_002523"
     CDS             4721..4960
                     /locus_tag="ACG8N9_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898069.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            5331..5612
                     /locus_tag="ACG8N9_002524"
     CDS             5331..5612
                     /locus_tag="ACG8N9_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4898070.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            5602..5943
                     /locus_tag="ACG8N9_002525"
     CDS             5602..5943
                     /locus_tag="ACG8N9_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4898071.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         2399 a          898 c         1295 g         1854 t
ORIGIN      
        1 aataaattgc ttgaagtatt atatttagat atcataattt atggaagaaa aattaattaa
       61 aaaggatgag attataatgg ggttactggt tccgagagct aatacttata ctataccttt
      121 aacgaaacgc caattaattg ctaaaagata tcaaaggatt actagagcca tcaatagaga
      181 gttttggaat tctgaaagtg atactgctca cagtttatac gtaggttctt atgggagagg
      241 aacggcaatt agtactagtg atatagatat aatagttgaa ttaccgatgg cagaatttga
      301 cagattcaaa aattatttat caaatggccc gtctaagtta ttacaagtta taaaaaacgc
      361 atttcaggaa atactaccaa atagtgatat tagagctgat ggacaagttg ttaaaataaa
      421 tttccatgat ggaatcaaat ttgaaatagt tccagctttt aatgaaaaag attattgggg
      481 tgagtcaaaa gggtttattt atccagattc aaacatggga gggaactgga aagctacaaa
      541 tcccaaaaaa gagcaagaag ccatgaaatt aaagaataca aaaagtaata atttattata
      601 tgctacgtgt aaacattttc gacatgtacg agatacagaa tttacgagtt atcatctttc
      661 aggaatagta attgacagtt tcgtatatga ggctatggga aattggaaat tcgttgaaaa
      721 taacagtggg ggtcaaaaca ttagttctgt atcttatgag acagctttat tagaatacta
      781 taactcacat aaagttatgg gcggtttaaa tttatactct cctggaagca atcaatttgt
      841 taattctgat agtagtatca tatgtttaga aaaagtatta aaaaaaatag ctttataaag
      901 ttttatggag gaacaacatg ggagacgaaa aaaagcgctt ggaagttcaa ttacgtgaag
      961 catatggacg agtaacgtat acttacacca ctcatttaaa gcaagccgat aatattattt
     1021 ctgacaataa aattttaagg tgtcttcaaa ttggtttgtc agcccttact actggagggg
     1081 tacttagtac cattattact gataatagtg agttaacctt tgttgctgga ttatgttcaa
     1141 caatttctct tggattaaac ttatacttta agaactttga tcttgctgaa gaagctaaaa
     1201 atcatcaaat tgttgccgat aaattatggt tgatcagaga agaatacgta agcttactaa
     1261 cagatttaaa tcgtttaagt gttgaagata ttgtgaaaaa aagagatcaa ttaatgaacg
     1321 aaactcatga agcatataaa aatgccccta aaacaaatag taaaagctat gaatcagctc
     1381 agaaagcatt aaaatctgaa gaagagcaat tttttacaga agaggaattg gataagatgt
     1441 taccagagca tcttagaaaa aagagttcta aatattgaac tcttttttta tgcttaaaat
     1501 tctcttgtaa tttttctatt caatcaataa ataatctata tttaagaaga aatatgatga
     1561 tagaaacatc tcctgaagcc accgcaagcc gttctaagca tagcttagcc cgacattgct
     1621 agatatgctc atctcaccat gaacgtttcc taaagccacc gcaagccgtc ctagccattg
     1681 cctagtccga catcgctaga ttagtgcatc tcgccataaa agttttctaa agccaccgca
     1741 agccgttcta agcatagctt agcccgacat tgctagatat gctcatctca ccatgaacgt
     1801 ttcctaaagc caccgcaagc cgtcctaagc atagcttagc ccgacattgc tagatatgct
     1861 catctcacca tgaacgtttc ctaaagccac cgcaagccgt tctaagcata gcttagcccg
     1921 acattgctag atatgctcat ctcaccatga acgtttccta aagccaccgc aaaccgtcct
     1981 agccatcgcc tagtccgatt ttgcttgatc ggttccattt ctgtttattt agtgggtcaa
     2041 caccgtaatc ttaaatagcc atcgcctatt taagattact aggtctgctt tgaaggtaaa
     2101 aagtctttca ttcttcaaga cttttaggct ttcaagctac gcctgaaagc attgaaattc
     2161 atcgctcttt atttggtttt gccatttata attccttacg cccattatct ttttcgcctg
     2221 tcttgccttg tcagaagtgt ttagagctta tctcatcttt ggattaatac aagtggcgta
     2281 aatttttttc taaacgcaag cgttacgaaa ataatttact taaacacttg tattaatccg
     2341 gcagataaga acgcattcta atcactcccg acaaggcaag acaggcgaaa aagataaata
     2401 aatgaacgaa aggaagaaac ataaaatgca aaaccaaata gcgatgaaga aaaaaccacc
     2461 tccctctaaa acaatccaaa caaaaaagaa ttttgaaaaa ccaaaaatca aaaacagaaa
     2521 gaagcgttca acatgagaga tagaatttta gaagttaatc aagaaatggt aaacaagtta
     2581 acaaatatca gtgggaacta tgaaaagtac gatggtactt tacttgataa ctatattttt
     2641 cacgatacac aaaatatcag aattggcaga agtaaaccga gagcgttcat tctcatcaaa
     2701 gagaaatatt tgaatgaatg gaatagcacc tatgaaatgg ttatgactga taactttgaa
     2761 aaggtattgg atttcagaaa tgctttgttg ggttctgata tgggaaatag cagtcttgaa
     2821 tctattgaag ttgaaaacaa gaccgagaat gaaggtgaag gattgattaa gaatgtcttg
     2881 aaaatcgaac aaaaatatat cagtgaagat agaagttttt atagagtcac aagctcggtt
     2941 ggtctagcaa actttgctac taacttaatt ggaaacaatg ctagtgaatc tttgctagtg
     3001 atgtgtctga atacaaagaa tgaggtcatt gcttatagcg aagttttcaa aggtggaatt
     3061 aatcaatcag tagcgatacc aagagagatt tttcaactag ctttgctaaa caattcagca
     3121 aaaatcgcag tatcacataa tcatccgagc ggatatgaat ttccaagtga aaatgacaaa
     3181 agttttacta cgaatttgaa aaacgcagga aaaacaattg gtattgaatt gttagaccat
     3241 atcatcgtaa caagtaaaaa cacaagctat tactcatttc aagaaaatgg ttctttataa
     3301 aaattagaga aaagaggaaa acaagatggg cgaaagaaca caagcgttaa ttacagtcaa
     3361 atatggtaac agcaaaacga aagcaagcgt gtcgattcat tatcagtggg ggtatggtcg
     3421 atatatgctg atggacgtat tgaatcaagg aatttattta atcaataaag catacgactt
     3481 acgagataaa tcaagagaag aattagcaaa aggaatcttt gatagaagta caggagttat
     3541 caattttgtt acctatgaat ctggagtgag tgcttatgat gaaacagggt ttggtgggcg
     3601 ctatgaacct tatcaagaaa cgattgaaga tgtgatgaag ttttcagata acaatgatgg
     3661 ctatgctcat ttagagatgg tgttcgatga aaatggattt agtctagatg ataaaaccag
     3721 attgcgattg tttgattttg agggtcaaca gataggacta aacaagtttt gtgaaagcgg
     3781 aaaagagttt gctacgaaag attttattaa gggatacaga ttattgctag aaaattatgg
     3841 aataaaccta gaagaagaga gcgtgaaata agtgtttgaa aaaagaaaag accagttccc
     3901 taatttagaa gcgaaaagtc aaaaagtgat tgatggtgaa atgattgaat tattccaaaa
     3961 aagttatgga accatagaaa ctaccgcaag ttgcaaatac tactttcaag gaaagagaaa
     4021 cacgtttaaa ggctttgccg attctgatga agaattggca aagaaaatca ttgattggct
     4081 tgtcagcagt gaacacgtag caagagaaga taccgttaga acaacgatta atggggaagt
     4141 cctaacaggt actagatgtg tttgttgtaa tcatgaattt gtagaagatg aaggttgcta
     4201 ccgaatcaat gaatatggtg attttatttg ccagagttgc cacgagatag aatttccaac
     4261 cgaaagaagt tggtttgaat acatgctaca agattatgag atggaaaaag aaaatggtga
     4321 agtagttttt tgtaccaaag aggaattgag taagagaagc gatgatgaaa ttgaagattg
     4381 gtgtgtgtgg ttagtagggg cagatgattg cgttgcatat tacacaacag cagaatagga
     4441 gtgtgaatca gcatggaagg aatggatttt ctagatcatg aagatttggt tgattttggt
     4501 tatacatgga aaggaatggt aggtattagc agaagtcttg ctaatgcctt ttacgaaaga
     4561 aattatgcag tatatgttct ttatgatgac gatacagaaa gccttgttga cgaggaatat
     4621 aaattggatt tagagaatgt tttatatgga atagaaaagg aagatttagc aaaatatatt
     4681 ttttcatggt tgggtcaata agtgaaagga agatagaaac atgacaaaat ttaaaagtaa
     4741 gggtatgcaa tggtgttctt attattatgt tggaaatgat gaagatttat tgagaaaaac
     4801 ccaacgaaaa ggttatccag ataagatatt gaaacaaata ggttattaca tgccagaaga
     4861 gaggatagaa aaagaatttt gtgaggttct ttttgatagt gaacagctat tagttttgtt
     4921 aacccaaaat aaattgattt gccaatttga aaataattaa gatgaggtgg tgaaccatga
     4981 atgaagataa gatggtatta agtgaatatg ctagattgag aactattaat tctttagcat
     5041 ctcatttaag ttgttctttc ctaacaaagg tgctaaaatt aatttacaaa tgtgaagatt
     5101 ttgtacaaga gaagcacaaa gacaagccta ctgcgaatag gcttgtcttt ttttcattca
     5161 ttacgattcc gtatgtgtgg gttagcgaca atttgttcaa ttaccgtatg atatagcatc
     5221 ttaatttgat tagattactt gcaatgtagt ttattaagag tataatataa tcataaatgt
     5281 ggacaatttg agaaataatg agtgttttat actcaaaagg agtggatgaa atgaaggtca
     5341 aaaatccaac tgaaaaaagc agaatccaag tcggaataga caagaattta aaagagaacg
     5401 cagaaatgat attggaagag ctaggtttaa atcctacaac agcaataaca attctttaca
     5461 aacaagtagt cgctagaggt gagtttccag tagagataaa gctaagtgaa gaggagaagc
     5521 aaggaatcag attgcaacag ctaactaagg acatgccagt agatgttcta gatacagatg
     5581 aaaagttaga agagtggttc gatgaagcct aatgagatag taacggtcta tgttgcattt
     5641 acagataaaa atggtggtaa aagaagacct attttggtgg ttagtgataa agaagataga
     5701 gtagaattct ttggaatcac aagtcaatat gagaaaaaat cagaccaaat aaagagggtg
     5761 tattttccta ttgcagaatg ggaaaaagca ggacttaaaa agcaatcatg gattgacgta
     5821 gggtctttaa aggctattcc caaaagccga gagaacatat cattcaaaaa ggttggtatg
     5881 ttatcagcta gtgatacgaa aaagctaaat aggtttatcg agaaattaca aagaaaagtc
     5941 taacaaggct tttctttttt gtttatatgt atattaagat atacgtttat ataaacgtat
     6001 ttatgtatat gaaaatatac gtaaatatat acgtgattat aaacattaag aaaacgttaa
     6061 gaattgttaa atagtgatat ttagtaatat acgaattata agatatattt tatatagaat
     6121 gtatgtaaat aaataaaatg tctgacggat attgaatagc aataaataac aataattcag
     6181 acggatacag tgagtgaagt agcttgctac gaacgaacag gcagaatgaa acggcagttt
     6241 cattagagca agtcgcttgc gacgtagcgt taccgctgat gtaactggca agttacatag
     6301 agagagtttg cttgcaaacg aacgttacct acttgaatag aaataaaaaa taacagatta
     6361 gtgaattttg aataggagcc acaactcaac caaccgcttg cggtgggtga gtcccacgga
     6421 ggaaaagacg aacgaacggt cgcgta
//
LOCUS       DAZUKD010000139         6301 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000139 DAZUKD010000000
VERSION     DAZUKD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.142"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8N9_002526"
     CDS             832..1566
                     /locus_tag="ACG8N9_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4898072.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8N9_002527"
     CDS             1582..2751
                     /locus_tag="ACG8N9_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4898073.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8N9_002528"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8N9_002528"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4898074.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8N9_002529"
     CDS             3841..4767
                     /locus_tag="ACG8N9_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4898075.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8N9_002530"
     CDS             4786..6036
                     /locus_tag="ACG8N9_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4898076.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
     gene            6167..>6301
                     /locus_tag="ACG8N9_002531"
     CDS             6167..>6301
                     /locus_tag="ACG8N9_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HFS4898077.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     L"
BASE COUNT         2112 a         1045 c         1283 g         1861 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagtat cgccgtttgt ataattaagc gagacaaata aaaaagccat ttatgttaca
     6121 ctcaaattgt aatacccgct aaagcataca aggagagtga acataaatga cttataccca
     6181 tcttacacca aacgagcttg taatgataga agcatatttt catcaagaaa ctccggttgc
     6241 tatcgttgcg aagcagctta aacgtggacg ccaaacaatt tacaatgtct ataactttct
     6301 c
//
LOCUS       DAZUKD010000140         6281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000140 DAZUKD010000000
VERSION     DAZUKD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.143"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..235)
                     /locus_tag="ACG8N9_002532"
     CDS             complement(<1..235)
                     /locus_tag="ACG8N9_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFS4898078.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNYIEELIKVIKFFEIKLIVP"
     gene            complement(247..2064)
                     /locus_tag="ACG8N9_002533"
     CDS             complement(247..2064)
                     /locus_tag="ACG8N9_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS4898079.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFIQD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2077..2841)
                     /locus_tag="ACG8N9_002534"
     CDS             complement(2077..2841)
                     /locus_tag="ACG8N9_002534"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4898080.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFRKNPNYLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIGKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2869..3567)
                     /locus_tag="ACG8N9_002535"
     CDS             complement(2869..3567)
                     /locus_tag="ACG8N9_002535"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4898081.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLSNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQIFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLSKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3579..4358)
                     /locus_tag="ACG8N9_002536"
     CDS             complement(3579..4358)
                     /locus_tag="ACG8N9_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4898082.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKEAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNGKTAPSRRSRSRV"
     gene            complement(4374..5318)
                     /locus_tag="ACG8N9_002537"
     CDS             complement(4374..5318)
                     /locus_tag="ACG8N9_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4898083.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5364..6143)
                     /locus_tag="ACG8N9_002538"
     CDS             complement(5364..6143)
                     /locus_tag="ACG8N9_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898084.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTIVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIDQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1649 a         1312 c         1128 g         2192 t
ORIGIN      
        1 taggaacaat taatttaatt tcaaaaaact ttattacttt tattaattcc tcaatataat
       61 tactttctga aactttaggt aatactattt tttcgtcaca catatatagt gcgggtgcta
      121 gttcagagca atctgctcca atgatttttc cttccatttc attattgttt aatgctgttt
      181 taaatgcatt cactagttca caacgacgtc cacaacttaa tattaaaata ttcaattttt
      241 aatctcctat ctattcgaag catttgcaaa agtgacaatg tctttagcca gttgttcatc
      301 gtcttgtggc aggctcttaa caaaatccat cactttttgt attgagtaac ctttgatgtt
      361 tcctacaaag attttctcat aaactgcttc atcatttctt tccttatcca acaatagttc
      421 ttcatatagt ttttcccctg gacggatacc tgtttcaatg atttcgattt cgtcttcgct
      481 atacccgctc aatcggatca tgtttttggc taggtctaca atcttcactg gttcactcat
      541 atcttgaata aaaatttcgc caccttttgc taatgcgcca gattgaataa ctaaacgact
      601 cgcttcagga atcgtcatga agtatcgggt catacgaaag tctgttacag tgattggacc
      661 accttgagca atttgttctc tgaatactgg aatcacacta ccgcgagaac caaggacatt
      721 cccaaatctg acagctgaga atttcgtaca gccttcttca ttcaatccag tcacgatcat
      781 ttcagcgatt cgttttgttg aacccatgac gtttggagga ttgttagctt tatctgtcga
      841 taccatgacg aagtttttca cttttgctgc tttagctgct tctgctacat tttttgtacc
      901 gaaaatattg tttttgactg cttcttttgg attatattcc atcagtggta catgcttatg
      961 agcagctgca tggtagacga tatctggatg atattgttcc atgatctcat tgattttctc
     1021 gcgatcttgg atatctgcga tgatcggtac gatttctgta ggacaatttt gatgatgcgt
     1081 gcgtaactca cggtcaatca agtagatcga gttctcccca tgtcctaaaa gcaatagctt
     1141 tgcaggattg aattgaatga tttgccgaca gatttctgaa ccgatcgatc ctcctgcgcc
     1201 ggtgacaaga atgactttgt ctgtgatctg atctttgatg gattcaatat ccagctttac
     1261 ctcatctctt cccagtaaat ccactacgtc aattgttttt aattttgata cgctaatttt
     1321 tcctgaagca agttcttcta tagaaggcat cgtgttgact ttcacacgag cggattcgac
     1381 taactcgaag attcttcgta atttttttct tgataaggaa gggatagcga tcgttaccat
     1441 ttgaatgtca tatttttcaa tcaattccgg aatatctttt aatgcaccta atactttttt
     1501 tcctgaaaga tacgtatttc ttttatttgg atcatcatct acgaacccga caacgtggat
     1561 atcttgagcc gttttggatc caagaaagct gttgtataaa atacgcccgc cttcgccagc
     1621 accaacaatc aatgtatttt tagcagaatc tgctgataca tatctcatat tttttgtttc
     1681 gatgtagata cgccagatca atcggctacc gatgatcaat aagagcgaaa gcagataagc
     1741 aaagataact aaccgtaaac tgtatcgccg attaatgaac agcagtatga ggatacttga
     1801 ggcagcactt gcagatagtg aactaaaaat tgcaatgatc tctcttaaat ttgtatagcg
     1861 attgattcga gtgaaaactt taaataaact accataaaat aaataaaaac caatcgacaa
     1921 accaatagag attaaaataa ggtccattgg tatagctaca aacggcttca tgaatttagc
     1981 tgctgcaata tttgcagcga ttaataacaa gctgtccaca acaaccaatg tggcaatttt
     2041 tcttcttctt gttaatacaa acaccctaca ttccccctaa aataaaccaa atttcttctt
     2101 ttttacctct tcatagactg gataactgat ttcatccccg ttgatcagat cctttgcgac
     2161 ttgtttcatc tgtactactt tttccttacc aaagtccttg ccaatctgct catagcactc
     2221 ttttaagtaa aaggtgcgct tagagacccc atgggcatca gaagcagcca tctgcacgag
     2281 attatgtttg accatcgttt tagctgtttt ctggatgtct ttgccaaaca ccccaacata
     2341 acttggcgca gtaagttggg ccaaacaccc catatctagg aacggaagca aataattcgg
     2401 gtttttacga aaatgcgcgt tacgttctgg atggacgatc actggtgtct ttcctagttc
     2461 taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg gaaattcgat
     2521 cagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga tgtcttcgat
     2581 gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca attgtctttt
     2641 ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta
     2701 ttttccatta ttgtggtgtg gtgtgcataa aatatgcgtg atgccttgct gaatcgcttt
     2761 ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat
     2821 atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt aatttccgta
     2881 gtaataacca gcatctttgc tatgttctgc tccgttgtat acaacaccta agactcgtgc
     2941 ttgtaccatc tctagcagtt cttttgcttt agacaaggat tctttacgtg atgtattttc
     3001 acgtacgacc aataaagtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc
     3061 tactactggt ggcatatcga atatcaccac gtcaaatatc tgtcttgctt cggctagaat
     3121 ctgatccatt cttggcgagc ttaacaattc tgatgggttg ggtggcttag gcccactagg
     3181 caaaatcgac aagttctcga ctggcgttcg ttggatcaca tctgctacac ttcctgatga
     3241 actaagtgct gtacttaatc cactagcatt gctcaactgg aatgttttat agacgacggg
     3301 tttacgtaaa tctgcatcga ctaatagaac ccgttgaccg gatttagcaa aaacgactgc
     3361 aatgtttgct gcagtggtgg attttccttc tccaggacct gatgaagtca cgacaatcgt
     3421 tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg tacgatattg
     3481 ttcggcgatt ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat gagtttgtgc
     3541 tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga gcggcttctg
     3601 cgtgatggcg ctgttttacc atttgtagct tttggtcgtg caggagctga tcctttttta
     3661 cggataccag cgttcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat
     3721 cctaatgtat ccgtcacata tttttcatct ttcactgtcc gatcgagtaa ttcgaataag
     3781 aatgccagac ctacgcctac catcattcct aatactaagc caatcactaa attcaattta
     3841 ttatttggcg aaacaggtgt cgtattggca actgcacctg aaataatcga gattttatcg
     3901 acattcatca cgtcttttgc gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt
     3961 tgagcagtca ctgcattcgt gcttattgcc tggatcgaga acatttgtga attttgtgac
     4021 tgattgactg agatggcctc tttgatttgt tcagcagtca ttttcacatt gtcttctgtt
     4081 tctaaacgat ctttgacttc atttagcact aaatcactga cgatcatatc tttatacgta
     4141 ttgatcattt gcaggttcgt attgacatca ttgacatttg ctgtgtctga ttgtggcagt
     4201 gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt
     4261 cctgagatgg ccaagccagc aaacattgag atgatgattg ttaccaagtg tttgcgtaat
     4321 gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta
     4381 ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcgctg
     4441 actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgcttg
     4501 atcgtaccaa atgcctcccg ataatctaaa gcaatttttt tcatttcatc aaagctcatg
     4561 tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt
     4621 agactcaata cttttttcgc tacaccttct acgatctttc tttgacgctc ttgtctacca
     4681 tagtcaccat ttggatcttc ataacgcata cgagaataag ataatgcctg atccccgttc
     4741 aaagtaattt taccgattgt aaagtcatag ccatcttgtg agaaagtgag gtcattattg
     4801 acttcgatac caccaactgc atcaacaagt tcttttagcc ctttcatgtt gatagagaca
     4861 tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg
     4921 ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac
     4981 gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc
     5041 atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatgcccatc
     5101 aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg
     5161 gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag atataatttt
     5221 gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat
     5281 atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct
     5341 tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat ttgctgagca
     5401 acagtttgtg cttttgttgc gtatgctgcg cgtatttttt cattccggat ctgatctatt
     5461 tgactgagca ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttc
     5521 aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgagt atcagcgaat
     5581 cctagatatt gcataacaat cgttttccaa ggactatctt taaaaaagct catattttca
     5641 cgtacacgag cgacatctgc acttgtcacc ttttctttga tgacgacatc gtctacagct
     5701 tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt
     5761 tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg gattgcaaca
     5821 gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag tttgacagag
     5881 gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt tagaaaattt
     5941 ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa ggtgtctttt
     6001 tgattggtga tggcagattc tgcataattg atccgttgct gatgttgccg ctgattattg
     6061 atatggtaac caaacaaaca gatcgcaata gcacctgcac ctaccgccac agcagtcaat
     6121 gacttgacga atggcttttc catagattca ctcctaagta ttgatgatag ataaattaag
     6181 atataattca cttacataaa aatgacagct ttttatttta aaaaacataa ttaaactaat
     6241 atattcacag aaatatttgt aaacaagttt tccctatata a
//
LOCUS       DAZUKD010000141         6237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000141 DAZUKD010000000
VERSION     DAZUKD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.144"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1219)
                     /locus_tag="ACG8N9_002539"
     CDS             complement(89..1219)
                     /locus_tag="ACG8N9_002539"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4898085.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1279..1506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1868..3070)
                     /locus_tag="ACG8N9_002540"
     CDS             complement(1868..3070)
                     /locus_tag="ACG8N9_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4898086.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3130..3333)
                     /locus_tag="ACG8N9_002541"
     CDS             complement(3130..3333)
                     /locus_tag="ACG8N9_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4898087.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3475..4539)
                     /locus_tag="ACG8N9_002542"
     CDS             complement(3475..4539)
                     /locus_tag="ACG8N9_002542"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4898088.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4600..4707)
                     /locus_tag="ACG8N9_002543"
     CDS             complement(4600..4707)
                     /locus_tag="ACG8N9_002543"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898089.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4870..5682
                     /locus_tag="ACG8N9_002544"
     CDS             4870..5682
                     /locus_tag="ACG8N9_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898090.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6056..>6237)
                     /locus_tag="ACG8N9_002545"
     CDS             complement(6056..>6237)
                     /locus_tag="ACG8N9_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898091.1"
                     /translation="FFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEFENAL
                     VICMTCTDEVFRKHF"
BASE COUNT         1843 a         1284 c          995 g         2115 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag ggaataagaa
     1561 caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat aaagcttttc
     1621 taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa atgatcatac
     1681 ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa aactaagtcc
     1741 tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac atcttgaaca
     1801 ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg gtagaaccgt
     1861 ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc tcttgaacat
     1921 tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg taagttccta
     1981 aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt tgaatattct
     2041 gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc ggtttgacac
     2101 ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc gtaaatgttc
     2161 cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca taccactgtc
     2221 tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat ttagttttgg
     2281 gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca atcgttatcg
     2341 tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca atccgaatgc
     2401 cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc ttatcatcct
     2461 tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact ttcttattct
     2521 cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc tctttaacag
     2581 cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat tttgataacg
     2641 tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt tcaaataacc
     2701 aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa aactgcatac
     2761 gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt ctctcgtacc
     2821 attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta gaacacagtt
     2881 gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa gcaattttgg
     2941 catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca ttttcatcta
     3001 tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa atatttttaa
     3061 tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat agtaccgcgc
     3121 ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc gggatggcac
     3181 cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa gatatgcctt
     3241 gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta tcaaaattgc
     3301 acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt ctttactcct
     3361 tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt tatactcgtc
     3421 ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc ttttttagta
     3481 gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa cctcctcctg
     3541 ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg gattcatttc
     3601 tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga cctgtgcaat
     3661 aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct caaaactttg
     3721 aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg ctgcccattt
     3781 ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa ttgctccatt
     3841 aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct ttgccaagct
     3901 ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg cctcttctaa
     3961 aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa aacgacactg
     4021 actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta tgatatccgt
     4081 accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta ttaccttatg
     4141 cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat caatccgtgt
     4201 aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg tttttcgtgt
     4261 ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca cagttccttt
     4321 aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt attggccaat
     4381 atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt caataatctc
     4441 ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat caatcaaaac
     4501 acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca atttttcttc
     4561 acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa tccccacttt
     4621 ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa tggcttcacc
     4681 gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt acttgtcaaa
     4741 gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta ttttaagtca
     4801 atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt atcctgagga
     4861 ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca gattcgccaa
     4921 gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc gcgaagtaca
     4981 gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca ccaaatcgca
     5041 gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa tcaatacatt
     5101 ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg tctcatagta
     5161 gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat gattgaattt
     5221 tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca atgtatcgat
     5281 gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca gctctatttg
     5341 caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc caactttagt
     5401 gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt tgggaaacat
     5461 ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca attgaccagc
     5521 gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca gtacacagag
     5581 gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat ggaagtcgag
     5641 aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa aattgataag
     5701 cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca cagtttcctg
     5761 ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg ctacgccttc
     5821 ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg acctggagtt
     5881 tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc ataatcagcg
     5941 cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct ggttagttaa
     6001 tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc aacctttaaa
     6061 aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca ttctcaaatt
     6121 ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat ttccattttt
     6181 gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg aaaaatt
//
LOCUS       DAZUKD010000142         6210 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000142 DAZUKD010000000
VERSION     DAZUKD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.145"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..324)
                     /gene="rpmB"
                     /locus_tag="ACG8N9_002546"
     CDS             complement(136..324)
                     /gene="rpmB"
                     /locus_tag="ACG8N9_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4898092.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            594..956
                     /locus_tag="ACG8N9_002547"
     CDS             594..956
                     /locus_tag="ACG8N9_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4898093.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1008..2690
                     /locus_tag="ACG8N9_002548"
     CDS             1008..2690
                     /locus_tag="ACG8N9_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4898094.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2807..4843
                     /gene="recG"
                     /locus_tag="ACG8N9_002549"
     CDS             2807..4843
                     /gene="recG"
                     /locus_tag="ACG8N9_002549"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4898095.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4892..5893
                     /gene="plsX"
                     /locus_tag="ACG8N9_002550"
     CDS             4892..5893
                     /gene="plsX"
                     /locus_tag="ACG8N9_002550"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4898096.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6015..>6210
                     /locus_tag="ACG8N9_002551"
     CDS             6015..>6210
                     /locus_tag="ACG8N9_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4898097.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQI"
BASE COUNT         2075 a         1079 c         1415 g         1641 t
ORIGIN      
        1 gaataaataa cttcataatt tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa
       61 tcaaaagtcc cacccttaaa aatttttatt aaataaaaaa ccgttcacta tctgaacggt
      121 ccaagtatca ttcaattaaa cgcgttcaac ttttcctgat ttcaaagcac gagttgacac
      181 ccaaactttt ttaggttttc cgtcgaccat aacgcgaact ttttgtaagt taggtttaac
      241 agtacgtttt gttgagttca tagcgtgtga acggttgttt ccactttttg tcttgcgacc
      301 tgtaaagtaa caaacttttg ccatggtaat ttcctccttt gccttgattt ggagcaatag
      361 aataattcag ctcataccag actaacatat catatagaat gggaatttgc aagtacattt
      421 tcatagatac aattgacaat tgagaagaat aattgtaaat taaacaaggt tatacctgaa
      481 attttgcaag gtttttgagt actttgggta actgcctttt atttcaagtt gcgctatgat
      541 aaaatatgat agaagaaaaa tgggagtttt ttacccgtta aggaggctca ttcatggctg
      601 taaaaattca aacgtcagct ggaaccattg aaattaccaa tgatgtaatt gcaacagttg
      661 ttggtggcgc cgcgacagat gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta
      721 aagataatat tactgagatt ttgcgtaaag aaaattactc aagaggagta gttgtccgcc
      781 aagaagataa tggtattgct gtagatgttt atacgattat cagctatggt acgaaaatct
      841 ctgaagtttc ccgcaacgtc caagaaaaag tgaaatacaa tctagaggca ttattaggcg
      901 taactgccaa ttcagttaat gtctttgtac aaggtgttcg tgtactgcca gactaggcag
      961 gagactggct ggtaactcag cagtcgatct gaaggaggat tatctaggtg agtgtaacag
     1021 aaataagtgc aagccagttc caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa
     1081 atgcagaata cgtcaattca ttaaatgtat tccctgtgcc agatggcgat acgggaacaa
     1141 atatgaatct atctatgact agtggagcta aggcagtagt agattcaacc tcagaaaaag
     1201 taggagaatt agcagcaatg ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg
     1261 gcgttatttt gtctcaattg tttcgtggat tttctaagca aattccagat gttgtctcat
     1321 taaatgcttc tgatcttgct gcagccttca ctcatggagt tgaaactgcg tataaagcag
     1381 taatgaaacc agtagaagga acgattttga cagtagctag agaagcagcg aaagctggag
     1441 aaaagaaagc aaaaagtact gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag
     1501 caaagcgtgc tttagctaaa actccagact tgcttccagt cctaaaagaa gtcggtgtcg
     1561 tagacagtgg agggcaagga ctgctgtttg tttatgaggg ctttttaagt gcattgaatg
     1621 gagaattcca agcggatgaa acatatgaac cttctccagc agaaatggat gatatggtca
     1681 atgcagaaca tcatcgcagt gtccaaggtc aaatggcgac agaagatatc cattttggtt
     1741 actgtacaga aatcatggta aaaatcggag aaggacctac agtcgatagt acattcgatt
     1801 acgaagaatt ccgtaactat ttagatggta taggagattc tttacttgtg gtcaatgatg
     1861 acgagatcat caaagtccac gtgcatacag aaaacccagg agaagtcttg aattatggac
     1921 aaaaattcgg ttcattagtc aaaatcaaag tcgacaacat gcgcttgcag catgagacga
     1981 ttttggaaca cgatgaagaa gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt
     2041 atggtatcgt agcgatcgct gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg
     2101 ctaattatgt tatcagtggt ggacaaacaa tgaatccaag tacggaagac attgttaaag
     2161 cgattcaaga agtaaacgca gataaagtga ttgtcttgcc aaacaataag aacatcttca
     2221 tggcagctga tcaagcagca gaagtagcag atatcccagt agcagttgtt ccttcaagaa
     2281 cgatttccca aggattgaca gctatgcttt cattcaatga tcaagcttct ttagaagaaa
     2341 acaaagaaac gatgacagaa atgttgtcta gcgttgtgag cggtcaagtc actcacgcga
     2401 tcagagatac ctcaattgac ggtgtagaga tcaaagaagg agactttttg ggcatgatcg
     2461 atggaaaaat cctgatctct aacccagata tcacagcaac agcgttaagt acactagaac
     2521 aaatgatcaa tgaggacaca gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg
     2581 aacaagcaaa tgaatttgct gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga
     2641 tccaccaagg agatcagcct gtctatcctt atctattttc tgctgaataa tttctgtgtc
     2701 agaatatctt tctaacacag tattcattca taaaaggcct tcggttagtg atcaggtatg
     2761 atcgctgacc aagtcctttt tttgttaaag gagggaggaa aaatttatgc gttctttaga
     2821 tgatccaatc actatgctgg caggagtcgg agaaaaaaga gcagctagtc ttgccagctt
     2881 gggcatccaa acgattgaag acttactgac ttattatcct tttcgctacg aagatattca
     2941 ggaaagaaat ctaaacgaga tccaggatca agaaaaggtt actttgaaag gaatcgtagt
     3001 ctcgcctcct gtgatgaatc gatttggtta taaaaaaagc cgattgcaat ttcgtatgat
     3061 gcaagaacat gatgtgttca acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt
     3121 cattttatcg gaggaaatcg ccgtatacgg taaatgggat gcaaagcgta aagccttgaa
     3181 cgggatgaag attttaggtt ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa
     3241 caaatcaatt cgtcaaacga cattagttga tctaatcaaa aaagggtttc aagaatacgg
     3301 cacattgatt gaagaaaatc tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg
     3361 agcttctgct gtaagaagta tgcattttcc aaaagaccac gaagaaaacc atcaagccaa
     3421 acgacgtgtc gtttttgaag agttcttttt atttcaaatg cgtcttcaag gattgaaaaa
     3481 agcagaaaaa gcagaaacga atggtttgga gatcctttat gatgtccaaa aattgaaaca
     3541 atttactcaa aagctacctt ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat
     3601 ctgtcgggat cttcgcagtc cgcagcatat gcagcgactt ttgcagggag atgtaggtag
     3661 cggaaaaaca gtggttgccg cgattgcgtt atacgctacc gttacagcag gtttccaagg
     3721 tgcgttgatg gtgcctacag aaatcttggc acaacaacat atggaaagct tgcaacagct
     3781 gtttgatccc aatgaagtaa ccatcgcatt gattacgggg tcaacgaaag caaaagaacg
     3841 acgtgagctc cttgaaaaac tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc
     3901 attgatccaa gatggagtcg aatttcagaa tttaggtcta gttattacgg atgagcagca
     3961 ccgttttggc gtgaaccagc ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt
     4021 atttatgaca gcgactccga ttcctagaac attagctatt acagcatatg gtgagatgga
     4081 tgtatctatc attgatgaac ttccagctgg acgtatcccg atcgaaacac gctgggtaag
     4141 aacacctcaa ttggacagtg tactggattg gacgaaaaaa gaactggcaa gaggacatca
     4201 aatgtatgtc atctgtccgc tgattgaaga atcagaagca ttagatgtaa aaaatgcagt
     4261 ggaaatctat gaaaaacttc gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca
     4321 tggtaaaatg aagaatcagg aaaaagaagt aatcatggaa gccttcaaag acaaccagat
     4381 gcagatatta gtttcgacta ctgtaattga agtaggggta aacgtgccga acgcgaccgt
     4441 catgctgatc atggatgcgg atcgatttgg attggcacag ctgcaccaac ttcgtggacg
     4501 tgttggacgc ggaagtgatg cttcctactg tatcttagtt gctaatccta aaaatgaact
     4561 aggtgtggaa cggatgaaaa tcatgacaga gaccaccaat ggttttgtac taagcgaaaa
     4621 agatctggaa ttacgtggtc ctggtgaagt atttggcttc cgtcaatctg gtctgcctca
     4681 atttgcgatt gctgacttgg tgacggatgg aaatgttttg gaggtagcca gagaagaggc
     4741 tgcagccctt tggcaaataa aggattggca gatgcttcct gagtatcagc ctttagcgca
     4801 aagcctccaa atagaaaatc aggataatcg ttatttcgat tagcagactg tgctatactt
     4861 tatactgatg acggaaaaag gagtggaaac aatgaaaatt gccgtagatg caatgggcgg
     4921 agatcacgca cctcaagcaa ttgtagaagg cgtggcttta gcgcagcaag attttccaga
     4981 agtagaattc ttactttacg gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa
     5041 aaacatcacg attatccata cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc
     5101 aattcgacga aaaaagactg catcaatggt ccttgctgca caagcagtga aaaacggtga
     5161 agcagatgct gtattttcag cagggaatac aggcgcgcta ctagcagcag ggttgttcat
     5221 cgtaggacgt atcaaaaata tcgaacgccc tggcttgatg tctaccttgc cagtagtagg
     5281 gaaagaaggt gccggtttcg atatgctgga tcttggcgct aacgcagaaa acaaaccaga
     5341 acatttgttg caatacggta ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg
     5401 acgtcctcgt gtcgcactct taaacaacgg aacagaagca acaaaaggta gtgaagtgac
     5461 gaaaaaagcc tatgaactcc ttcagaatga aacaagcttg aattttattg gaaacgtaga
     5521 agctcgtgag ctactcaatg gtgtggctga tgtagtggtc acagatggat ttacaggcaa
     5581 cgctgttttg aaatctattg aaggaacagc aatgaatatg atgaatctat taaaatctgc
     5641 tattctaaat gaagggatca aaggaaaaat gggcgctatg cttttaaaag acggcttacg
     5701 ttctttgaaa gcagaaatgg attattccaa acatggcggt gccgttttgt ttggcttgaa
     5761 agcaccagtc attaaaactc atggagcaac aggccccgat gctgttcgct atacgatccg
     5821 ccaaatccac acaatgcttg agacagatgt agttggacaa ttagtcgaac aatttgaaaa
     5881 aagcgaagaa tagtatttgg atatctgaag cgatccgttc cattgaaaaa aaggtggaca
     5941 ttcctgaaaa atgccagtaa aattagccat ggtgtaggta attgacaagt gtatgattct
     6001 tggaggtgaa aaagttgact cgcgaagaag tatttaacaa agtagcgaaa attatttcaa
     6061 atcattttga gatagatacc gataaagtga cggatgaatt gaacatcaaa gatgatttaa
     6121 aagctgattc aattagtatt atggaattcg ttttagagct tgaagatgaa ttcggtacgg
     6181 aaatttcaga tgaagacgct gaacaaattg
//
LOCUS       DAZUKD010000143         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000143 DAZUKD010000000
VERSION     DAZUKD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.146"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8N9_002552"
     CDS             725..811
                     /locus_tag="ACG8N9_002552"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4898098.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8N9_002553"
     CDS             complement(932..1621)
                     /locus_tag="ACG8N9_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898099.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG8N9_002554"
     CDS             complement(1628..1906)
                     /locus_tag="ACG8N9_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898100.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG8N9_002555"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8N9_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4898101.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8N9_002556"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8N9_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4898102.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8N9_002557"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8N9_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4898103.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8N9_002558"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8N9_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4898104.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="ACG8N9_002559"
     CDS             complement(4578..4793)
                     /locus_tag="ACG8N9_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898105.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8N9_002560"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8N9_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4898106.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8N9_002561"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8N9_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4898107.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1632 a         1151 c         1042 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga atgactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUKD010000144         6130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000144 DAZUKD010000000
VERSION     DAZUKD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.147"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..222)
                     /locus_tag="ACG8N9_002562"
     CDS             complement(<1..222)
                     /locus_tag="ACG8N9_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898108.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGK"
     gene            366..927
                     /locus_tag="ACG8N9_002563"
                     /pseudo
     CDS             366..927
                     /locus_tag="ACG8N9_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1038..2012)
                     /gene="lacD"
                     /locus_tag="ACG8N9_002564"
     CDS             complement(1038..2012)
                     /gene="lacD"
                     /locus_tag="ACG8N9_002564"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4898109.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(2031..2486)
                     /locus_tag="ACG8N9_002565"
     CDS             complement(2031..2486)
                     /locus_tag="ACG8N9_002565"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4898110.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(2514..3959)
                     /locus_tag="ACG8N9_002566"
     CDS             complement(2514..3959)
                     /locus_tag="ACG8N9_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4898111.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(3956..4891)
                     /gene="pfkB"
                     /locus_tag="ACG8N9_002567"
     CDS             complement(3956..4891)
                     /gene="pfkB"
                     /locus_tag="ACG8N9_002567"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4898112.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            5059..5817
                     /locus_tag="ACG8N9_002568"
     CDS             5059..5817
                     /locus_tag="ACG8N9_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4898113.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT         1914 a         1306 c         1001 g         1909 t
ORIGIN      
        1 ttttcctttg atgtctgcta ataaaacacc tgccaactca tcatccgcat ataaagcaat
       61 gatttgtgtc gcgcgattca attctagata ccaaaagtat cttccataaa gattcaaaag
      121 gagtttgttc ttggtatacc aatcaaagtg cataccttta atcgcgaatt gaatggcttt
      181 tttgtagtct ttcttttcca gttgcttaaa ggctaattcc atcacttatt ctcctattct
      241 ttaaatattg atatgctata cttatattat aatagacaag gtgtcggtta tctatgttca
      301 gatagcggtg aaattgtcgg ataaccgaca caattccttg attatgttga ttaggaggct
      361 gcaaaatgaa aaaagaggat cgccgcgtca aaaaaacaga aaaagcgtta gcagaagcat
      421 tatcaaaatt attagtagat aaaaaaattc aaaacatcac catccgtgaa ctgaccgaaa
      481 ctgctgatct tcaccgttcg acattttata cacattatac ggatatctat gatctttatg
      541 aaaaactgga aaatagcttt ttccaagact tggatgaaat catttcctac gatacgacac
      601 atagctacga ggatctatac gctcaattga tcgactatgt ctatacgaat acgttcgtat
      661 acaagttatt cgtcatcaac cacggagata tccaatttca aaagagatta aataatctga
      721 tcgaagaaaa ttatttaaaa atctggctac aagaagacaa aaaaaccgtg atcacagaag
      781 aaatgcgtta cttcaccatc taccatatcc aagggtgcct ctccgtttta aatcattggg
      841 tagaaaagca attttgcata ctctaagaaa aaaatgctga ccttcttgcg cttattgaac
      901 gacaacatag aacctatcat gccttaaaaa caaacgaaaa aggaagcgtc gggacgcttc
      961 caattcttta tttaaaagct caaattgatt attaaaaatc attaggttac aaacttaagg
     1021 ttatagttaa agctttctta caatttaata gctgttgctg tttctttcaa aacattattc
     1081 agctcttcga tgttcttgcg tccttgtgtc tgcatccatt ctttggcagc atattcacct
     1141 ttagtaacga atggttctac accattcgcc caagttgcgc gcccacagag taccccattg
     1201 aatgtagagc cagcctcttt tgcaaatttc aaggtttctt ggaacaattc agcagttact
     1261 cctgcgctca agaaaataaa tggcaggttg gtagtctcgg attgttcttt gaaataagca
     1321 gcagcttctt ctttggtata agcaacttca tttttagtgt aaccatccac atagttcata
     1381 ttaacaggaa cttccacttt caaaacgtca actccatagc gaggcttaga aaattccttc
     1441 atcatttcat ttactttatg cggttttttc aaggcatatt ccttcgaaga agtatcagga
     1501 tcgttggcat cataagaaac caattctaag aaaaatggca aatcttcagc aacacattca
     1561 gaaccaatcc gctccataaa cgccttcttt tggtcattga tgtcatcaga ttcatctaca
     1621 tcgtagtata acaagaactt acaagcatcg gccccttgct cctttagacg ttttacagac
     1681 caaatagata aaatatccgg taaacgtcct ggagttgaag catcgtaacc agttttttca
     1741 taggccaaca acagaccagc atttttatcg cgaacttcgg ctgccggcaa cccatattct
     1801 ggatctaata agattgctga agcatatgga gtcaattcag aagaaatgat ttttttgaaa
     1861 ttgatgattt gttcatcggt tgctgattta ttttgaaatt taccgatcat ttttttcaag
     1921 gcaccacgtt gatcgattgc taacgcactg atgatgccac tttcattact tagttgacga
     1981 atataagttt tctttttttc tgttaaaggc atgatttcac tacttccttt ttagttttct
     2041 tctaattttt ggtttaaaag ttcagctatt tcttcagcag actgggcttg tttcagacct
     2101 tctgtcacat ctttgtgcat caaaagtctg gctacctgag ataaaaattt tagatgggtt
     2161 gttccagcct ctgaatctgg aataaacaga gcgattacaa aatccgttcc ttctccatct
     2221 aaacttttcc aatcgacctt accgtttaac cgaacaatca caatatcggc atccttgata
     2281 ctagcatctt tggcatgagg gatggcaaaa ccatccatca taccagtcgt gctttcttct
     2341 tctctttcca tcaatttttc gtaaactgca tctcggtctt cactaatgct caagacaatt
     2401 gactgatctg caaggaagtt cattacctca tcttgcgtcg taaaattttt attcataaaa
     2461 actttatttg ttttgatcaa agtcatttga aatccttctt tctagaataa cctttagttg
     2521 ttggttgctt ctttcatttc aacagcagct gcttctgctt cacgtttttc tacactacgt
     2581 ttttggatca accctaaaat tactccgcca acgattgaac caagtaaaat cgaccctacc
     2641 catgcaacct taccagtaac aacaggtaat actagaaaac ctccatgagg tgcgggaact
     2701 tggacaccaa acatatatgt cagtacagca gcaattgatg aacctagcat catagttggc
     2761 agaacacgca gcggatcttt agccgcaaat ggaattgctc cttcggtaat gtgcgttgag
     2821 cccaagataa agttgactaa accagcgttg cgatcattcg tgtcgaaata ttttccaaaa
     2881 aacagtgcgg caaatccagt aatcaatggg ggcgcgatac aagctgccga aacccctgcc
     2941 ataaatgtat ggttaccttg tgccaacaac gcagtccctg taacataagc cgctttattg
     3001 actggacctc ccatatcaaa tgctgacata cagccaacga taatccctaa aattaatgga
     3061 ttagaatttt ggaatcctgc caagaaattc atcattcctt ggttgactgc ttccattgga
     3121 tttgctaata aagacatgac tacaccaaca aacaagacac cgatcaccgg atataagaag
     3181 atcgccttca gaccatccaa ttgtttaggc aagcctttca tgaccttttg caatagtaag
     3241 ataaagtagc ctgctaagaa acctgcaaaa attccgccta agaaaccggc tccgccagaa
     3301 ttggctaaca gtccgccaac aaacccaaca accaaaccag gacgtttagc gattccttca
     3361 gcgatataag ctgctaaaat aggaaccatc attcccattg ccgcatcacc aacacccttt
     3421 aacatagcgg cagtcggatt gtaagtagga tcattgggat caaaagagta cacaccccag
     3481 atggcaaacg aaattgcaat aattacccca cctgccacta caaacggcag catatgagaa
     3541 acaccgttca tgagattttt ataaatactg tgaccaatgc ctgctgcaac ccccatctcc
     3601 gctttctcac cagcagcgtc aactgttttg ttttctgcag tgattgaacc tttaccagcc
     3661 aacgcatctt cgatcaattg gtcagcatct ttaatcccct tgctgacagg cacatcgatt
     3721 acacgcttac ctgcgaaacg ttctgcttga acatctttgt cagctgcaat gattacagcg
     3781 tcagcgtttt ttatttcttc tggtgttaat tcattctcta tccctacttg accatgggtt
     3841 tcaactttga tagatacccc tttgcgctta gctgcctgct ccaatgcttc ttgagccata
     3901 taagtgtggg caattcctgt tggacatcct gttgcggcaa caattttgta agttgtcatt
     3961 ttttactcgc tccctctttg atttcgattt gtttttccaa ctctgcaaca tccgaaaaat
     4021 ctgttaatcc tttacgaaaa gcggttgaac ttccagctgc taaacttttc ttcaaattaa
     4081 tatctacctg ttcacctttt aaatatcctg caagaaatgt tcccaacaaa gtgtcaccag
     4141 cacacgctgt attgactact ttacctttag gagaattccc agaataaacg atatccttat
     4201 ttaaaaatat tcctccctct cctcctagag aaaggagaac atttttcgct ccatcggcaa
     4261 tcaagcgttc agcataatga atatatcctt cacgatcact aagctcgaca ttataccaag
     4321 attgcaattc ctcctcgttt ggttttaata agaacggctg atacggcaga caatcaatta
     4381 tttccggata gcttgaatca ataatcagat ggaccttatt ttcaaaacaa atacgactga
     4441 tatcaataag aatttcgggt gagacccctc gcggcaaact gccagaaaca cacaaataat
     4501 cgtcagcttg cagcttttgt atttgattta gaaaagtttt gatttgttcc tgaggaatct
     4561 caggtccttt attaaccaat ttgaattctt cattggtttt attaacttgt gtaaaaacgt
     4621 tgattctagt cattcccggt acgctaataa aatcagtgct aatatctttt tgatgcaaat
     4681 aatcctcaat ataattcccg gtaaaacctg ctttaaatcc taaagctgta ctatcgatac
     4741 ctaattgctt catgatcaat gaaacattca caccttttcc attagcttgt atatcatcat
     4801 cgtttgttcg attgacttgg gatggtttca tttcatctgt ttcgataaaa aggtcgattg
     4861 ccaagtttaa agtacatgta taaactgaca tttacgctcc tcctttctta catattcatt
     4921 atgtatcaag atcgaatgta aatgttttca taaatgacta aattcattga aaacttttac
     4981 aaaatatatt aaataaactt gaagtaaagg aaactcccgc tttacaatag aattatccta
     5041 actaatcgga ggcaaaatat ggatacaaaa ttaagtgaat cggagaaata tctctgggat
     5101 tttattcaaa gtcacatact cgaaatcccc tcctattcca ttgtaaaact aagtgaatat
     5161 gccaacgttt caacagcaac cgtcgttcgt actatgaaga aaaaaggata tgaggggttt
     5221 acgtctttta aacattcgtt aaaagaaaaa gaaaatacaa atatcaattt tgccgctctt
     5281 gataaagttg atgaaggaat aaagactgct attcttaaaa atgagcaaga ggtaatccgc
     5341 accatcaata tggtagaaag cgggaatatt gaagatgcta ttcaacgaat caaggctgct
     5401 agaagaatca tgatttttgc ccgaggtttt tcagaattga ttgctcagga aatgatggtc
     5461 aaatttcaac ttaccggtaa atattgtgag atgcatactg atcccaatat cataaaaaac
     5521 attagtagaa aattatccaa agatgacatc gtgatctttg tttcactaaa cggtgaaaca
     5581 aaggaattga ctaccgcagc tgaaaactgt tataaagctg agatcgggac aattttgctt
     5641 actgctaatc gtcattcaag tttgatggaa catatcgaga ttccttttgt tggctttaaa
     5701 tcagaaggct ccttcttccc agattatgag gtacgctcca gacttccact ttctatactg
     5761 gctagaatct tacttgactc ctatgcactg cgaacatcag gattcacaca gcactaactc
     5821 tatatctgta agactgtacc taaaatatat ctgtgtgaat taactaaggt tgtctagaaa
     5881 aatatttaat cgctatttgt attctcgtgc tttctatcat tgctccttct ataacttctt
     5941 agtttcaaga tagaatagtg cattgagact accaggacta cttacgcaaa tatataataa
     6001 taagaatgtg ataagcagtt cgaagttcct gtctaatatt tcttgttaat tattatcaat
     6061 ttccaacaat tctagaatta taatacgcaa aaaaaggaga ccgcctaacc gtctccttat
     6121 agtttattat
//
LOCUS       DAZUKD010000145         6120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000145 DAZUKD010000000
VERSION     DAZUKD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.148"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..525
                     /locus_tag="ACG8N9_002569"
     CDS             7..525
                     /locus_tag="ACG8N9_002569"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4898114.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            653..1336
                     /locus_tag="ACG8N9_002570"
     CDS             653..1336
                     /locus_tag="ACG8N9_002570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4898115.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1645..1718
                     /locus_tag="ACG8N9_002571"
     tRNA            1645..1718
                     /locus_tag="ACG8N9_002571"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1679..1681,aa:Arg,seq:tct)
     gene            1877..2236
                     /locus_tag="ACG8N9_002572"
     CDS             1877..2236
                     /locus_tag="ACG8N9_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4898116.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2359..3990
                     /locus_tag="ACG8N9_002573"
     CDS             2359..3990
                     /locus_tag="ACG8N9_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4898117.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4052..4693)
                     /locus_tag="ACG8N9_002574"
     CDS             complement(4052..4693)
                     /locus_tag="ACG8N9_002574"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4898118.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4879..4955
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4975..5283
                     /gene="rplU"
                     /locus_tag="ACG8N9_002575"
     CDS             4975..5283
                     /gene="rplU"
                     /locus_tag="ACG8N9_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4898119.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5310..5654
                     /locus_tag="ACG8N9_002576"
     CDS             5310..5654
                     /locus_tag="ACG8N9_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4898120.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5667..5960
                     /gene="rpmA"
                     /locus_tag="ACG8N9_002577"
     CDS             5667..5960
                     /gene="rpmA"
                     /locus_tag="ACG8N9_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4898121.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1897 a         1025 c         1301 g         1897 t
ORIGIN      
        1 aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt gattatattt
       61 ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt
      121 attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg
      181 aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa
      241 aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa
      301 gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt
      361 cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt
      421 caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt
      481 gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat
      541 aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc
      601 agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa
      661 tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca
      721 aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc
      781 agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga
      841 acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat
      901 cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa
      961 aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc
     1021 agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc
     1081 aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat
     1141 ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg
     1201 caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga
     1261 tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga
     1321 aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc
     1381 tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa
     1441 tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta
     1501 gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa
     1561 ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta attatctata
     1621 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
     1681 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
     1741 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
     1801 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
     1861 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
     1921 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
     1981 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
     2041 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
     2101 atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag
     2161 tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct
     2221 ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa
     2281 aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat
     2341 gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca
     2401 ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta
     2461 gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg
     2521 attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata
     2581 tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg
     2641 caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc
     2701 cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt
     2761 attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt
     2821 acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc
     2881 tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa aaaatgggcg
     2941 aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc
     3001 catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg
     3061 aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt
     3121 gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat
     3181 aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct
     3241 ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct
     3301 ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt
     3361 gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt tttgattgtg
     3421 acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg
     3481 tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt
     3541 gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc
     3601 acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg
     3661 aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc
     3721 aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta
     3781 ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa
     3841 agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac
     3901 ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta
     3961 catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa
     4021 actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta
     4081 cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat
     4141 gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg
     4201 ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt
     4261 tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct
     4321 tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc
     4381 gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat
     4441 acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag
     4501 aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct
     4561 ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag
     4621 cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt
     4681 ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat
     4741 gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt
     4801 tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta
     4861 ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata
     4921 tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac
     4981 gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt
     5041 gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc
     5101 ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt
     5161 gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca
     5221 caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct
     5281 taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg
     5341 caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg
     5401 atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat
     5461 tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg
     5521 aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc
     5581 ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa
     5641 ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc
     5701 acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg
     5761 ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta
     5821 caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag
     5881 ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc
     5941 cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca agacttttgt
     6001 cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag tttactttgt
     6061 ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat aactatcgtt
//
LOCUS       DAZUKD010000146         6066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000146 DAZUKD010000000
VERSION     DAZUKD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.149"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            437..643
                     /locus_tag="ACG8N9_002578"
     CDS             437..643
                     /locus_tag="ACG8N9_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4898122.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            643..894
                     /locus_tag="ACG8N9_002579"
     CDS             643..894
                     /locus_tag="ACG8N9_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898123.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            909..1346
                     /locus_tag="ACG8N9_002580"
     CDS             909..1346
                     /locus_tag="ACG8N9_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898124.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            1339..2046
                     /locus_tag="ACG8N9_002581"
     CDS             1339..2046
                     /locus_tag="ACG8N9_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898125.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            2211..2411
                     /locus_tag="ACG8N9_002582"
     CDS             2211..2411
                     /locus_tag="ACG8N9_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898126.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            2427..2606
                     /locus_tag="ACG8N9_002583"
     CDS             2427..2606
                     /locus_tag="ACG8N9_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898127.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            2947..3210
                     /locus_tag="ACG8N9_002584"
     CDS             2947..3210
                     /locus_tag="ACG8N9_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS4898128.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            3204..3554
                     /locus_tag="ACG8N9_002585"
     CDS             3204..3554
                     /locus_tag="ACG8N9_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4898129.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3578..4192)
                     /locus_tag="ACG8N9_002586"
     CDS             complement(3578..4192)
                     /locus_tag="ACG8N9_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4898130.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            4506..4928
                     /locus_tag="ACG8N9_002587"
     CDS             4506..4928
                     /locus_tag="ACG8N9_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898131.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            4938..5141
                     /locus_tag="ACG8N9_002588"
     CDS             4938..5141
                     /locus_tag="ACG8N9_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898132.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            5352..5936
                     /locus_tag="ACG8N9_002589"
     CDS             5352..5936
                     /locus_tag="ACG8N9_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4898133.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         2138 a          944 c         1157 g         1827 t
ORIGIN      
        1 aagaagagtt gtagtcctaa atgaggattg cagctctttt tgttttacat agaaacagga
       61 aaagaccttt ttaggaagac acaagtgcct tctcaattga aaaacctttc tctcactcca
      121 accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg
      181 tcttgcaaag caagacaaaa tagacaaaac ttcctctcac caaaaggttc gttcctgttc
      241 gttatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga
      301 caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattta
      361 ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga
      421 ggaggcagaa tcaaacatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt
      481 attcccaaac tatcatgcac cacatactgt tgaatgcgat tgtggagaaa gagcgaaatg
      541 tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta
      601 tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa
      661 ttattgactg taaaagaagt cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa
      721 gtagcagaag ctttacttat tccgcggtac tggacgatag aaaccccttt agtagaggat
      781 gcaccgacta tgctatttga agacctagtg aagattgcag gatatgaact gcattatcaa
      841 gaaaacatgg aaacttatga aatcgaatat tggttaacca aattagagaa ctaaaaagga
      901 gaaaaaatat ggctcaaaat tactatgacg aatttgtcaa attacctttg gataaaatgg
      961 cacaaaaaat ggaggatatg acgttcctat accatgaaac acgtgtaccc aaaaaacatt
     1021 acaaggaaaa attgtcagtc gcagtggaag aaatgattga atcaggtgtc gaaatgaatc
     1081 tcattgcgac gtactatcga acactggaag aactgaaaaa acaaaatgga aaatggtttt
     1141 tccaagcctt actatgcttg gaagtaggtg tcaaaccaag cactatcaaa ccttccgaat
     1201 atcaagcctt agaactgact tatgcaaaat tcgcacagat taagaaagct aaagcaatgg
     1261 ctagcgactg gctagatact tttgaaagta tcgaaaaaaa tggtgcattt tatacgattg
     1321 aatcggagga gaaagataat gaataaggtg gtttatcagc taacaacaga agatgtttcg
     1381 caatttttaa caagcaaaga acacttgaat gaagtaatca gacggattca aaaagacttt
     1441 agttgtatgg aagaaatgaa gtactttatt caaagagtgg aagaacagtt gtatatggaa
     1501 agcgaaacaa gtcaaacgaa ggtcacagtc agccataatc atttagacgt gtataaacgt
     1561 cgtcctgaaa cggtaaaaaa aggaacagag cttctacgac tagaaacaga aaaatacatc
     1621 atgaatgtgt acctagaaag gagtgagctg gtcgaaaact ttgtcagcta cacgatttgt
     1681 aggaaacact attactcaga atcagaatat tattcaattt tcgagagcat ggaattaatg
     1741 gaagaagaaa tggaagagct aaacagcaaa aatgcgacgg tagacgatta ttaccttgta
     1801 tttactaggt ttttgacgtg tgggacggaa tctatcgtgt taatgggaat ggattatgaa
     1861 ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag aagtagataa cccaggcgac
     1921 gactggtaca cgactttaac tgatttttta gacacctaca cttacgacga tacagaagaa
     1981 atccaaaatg aacttgaccg taaacgaatg ccatatcaac gatttgaaag gaaaaaaggt
     2041 aaataaccat ttataccgaa aagtgaggat gtgtgaaata agaacaaaaa accttgaaat
     2101 taagggaatg gttcttattt cgcaccaatc gctccaaccg cccccgccgc ttaggtggtg
     2161 cgaaataagc gggcaaagcc cgataaccta agagaaagaa ggagatccat atgaataaaa
     2221 tcattcctct attgttcgta ctgtttgttg gcgagatact tatgcgtcat gctgcagctg
     2281 tacatttaaa cttcgtagta ttcttaggaa tactggtatt ttgtttgatc ttttggcgat
     2341 ttgtattagc ccttgtgagt ctactattca agatgattgc agccatattt ttcatagggt
     2401 tatttcgatg aaaggagaga gtactaatgc caattgcaat aggaaataaa cggttacccg
     2461 tgacattaga tgaaaagaga caaaaagaat tgcagcaact aaagcagaag tacggaaaaa
     2521 gtgaatctaa gattatgtgt attgcgttag atctattgat cgcgcaagaa aaagcaggat
     2581 ttgatatacc agcactaaaa aagtgacgtc acttttcatt tgaagaaaca aaagtgatag
     2641 cacttaattg ttcctactat ttagaatatt ttctatgtcc tttttattcc attttgtatc
     2701 aataactatg aaatctttaa agtatccaga agagattatt tttagatcaa tttttttctt
     2761 acctgattct aaagattttg ttttcttaaa catattatga tttcctttcg actattttca
     2821 tataaatgtt aacatagttt atggattcat ggaatgttaa tctaatttaa tagaaaagga
     2881 taattaagta tatacgtttt ttcttatgta tgttatacta tgtatataca gaaagaggtg
     2941 tctactatgg aaatgttacc tataaaaaaa tggggaaata gtaatggcct tagattgcca
     3001 aagtatatta tggaatatct tggaattcat acagaagaca aagtgaagat aatacaagaa
     3061 gaagtaaacg gtcagaagcg gctaattatc gaagcggcta atatagaaaa tgaattaaca
     3121 attgaacagt tatttgaaaa ttacactgat gaaagaaatc atgtgacgat tcaaaattta
     3181 ggtgaagcag taggtaatga aaaatggtga gaaaacctag acaaggcgat attctattat
     3241 tgaatacagc acctcgatct ggacacgaac agacaggtaa aagaccttat attgttttaa
     3301 gtcatgatat tattgcggat tatagtaacg ttgttacagt tgctccaatt tctagtacaa
     3361 ctagaaacta tccgctatat gtaaatataa actcagaata tgaaatgaag acaacaggaa
     3421 aagtattatt agatcaacta acgacaattg attatgaagc cagagagtgt atttttttgg
     3481 aaaaagcgca tgatagttta gttgaggagt tattgatgaa agttagaaca gtttttcaaa
     3541 aagtaagcaa ataaaataga aacagcagca tgatcaacta ttcgtgattg accatgctgt
     3601 ctttttttat tctatatacg gtttgtctcg taacattgta gtcttttgca atcttagcga
     3661 tcgcaacacc tttttttaga tcctcaacga tgtttttata aacaagacga cgttggggat
     3721 cttttgcatt ggcactgtat agcttaggac gtcctttata aacaccgttc gcttttgcaa
     3781 tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg ttcttgttca gcgatcatgg
     3841 ccaaaatttg aatgatcaaa tctttaatga atttatccag gagaggattg ccaattgctt
     3901 cggccatgat cggtagactt gtgatgatca gctgcacatt tttcttcttc aagtaattga
     3961 ctgaagcgat gatctcgtcg taatttcttc caagtcgatc aatagcttcc acaataaaaa
     4021 tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac aagtcgatga gtaatcgttg
     4081 cgccagattg tttctcaatg aaaattctct ctgcaccgaa ttttttcatt tcttcaatct
     4141 gccgttgttc attttgatcg atggatgaga cacgtatgta tgcaactctc aaaattttta
     4201 cctactttct gcaactattt aaaaaacacc ctaatcatac acgagaaaac cagatttttc
     4261 aatgtcgcgg tcgtgtctag ttagggtata ccttatatag acaaaaacta atgatggagg
     4321 aaatagtatg tgtcactttt ctttttaaat ggcaatattt tttgtaaata aatgtcaatc
     4381 aagaaaattt aaacgattat gacattaaaa atatatttta tgaattttct agtatataac
     4441 ttgatttatc tgttaagatt tattaagaaa acgttttctt atttttgatt tcgaggtgat
     4501 agacaatgaa aaataagttt attttatctt taggtatttt aactattgga attagtgtaa
     4561 ctggaactac aattcctaca tttgctgata gtacaaatga accaactact gtagtagata
     4621 ataaccaaga attaactcct ttagatagga acggaatagc cattggtaac ccaactaacg
     4681 acttaactgt tactgaaaat tatttaatag tttctccatt acttgctcga gcagcttcta
     4741 aaacgatacg agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc
     4801 ctcaatcacg gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt
     4861 tacaaagtta taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta
     4921 gaaagtaggg attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg
     4981 atattctaag taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt
     5041 tacaggagac acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa
     5101 gaaaaaaaag agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg
     5161 tctagtcgac attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca
     5221 ctttcttttt ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa
     5281 caatatctat gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag
     5341 gagtgatttt tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt
     5401 tacaagaaga cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg
     5461 gggcaaaaag taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata
     5521 cacttgtcgt ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg
     5581 tcaatcgact gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg
     5641 acaaatcaag ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg
     5701 agcgtaattt gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac
     5761 ggtttggtgg tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag
     5821 tggatagcgg tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca
     5881 tttatcgtta tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt
     5941 tgtcatttaa tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat
     6001 tttttgtaag tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt
     6061 taatat
//
LOCUS       DAZUKD010000147         6061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000147 DAZUKD010000000
VERSION     DAZUKD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.150"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..589)
                     /locus_tag="ACG8N9_002590"
     CDS             complement(68..589)
                     /locus_tag="ACG8N9_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4898134.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(591..1625)
                     /gene="sppA"
                     /locus_tag="ACG8N9_002591"
     CDS             complement(591..1625)
                     /gene="sppA"
                     /locus_tag="ACG8N9_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4898135.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1658..3073)
                     /locus_tag="ACG8N9_002592"
     CDS             complement(1658..3073)
                     /locus_tag="ACG8N9_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4898136.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3042..5195)
                     /locus_tag="ACG8N9_002593"
     CDS             complement(3042..5195)
                     /locus_tag="ACG8N9_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS4898137.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5453..5689
                     /locus_tag="ACG8N9_002594"
     CDS             5453..5689
                     /locus_tag="ACG8N9_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4898138.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5708..5923
                     /locus_tag="ACG8N9_002595"
     CDS             5708..5923
                     /locus_tag="ACG8N9_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS4898139.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         1899 a         1108 c          908 g         2146 t
ORIGIN      
        1 aaaagactac ataaaatccc tgttattcca ctaattaaaa tcaaaataaa ctctgttttt
       61 ctcttattca tttaattctc tccagattaa gtgttttaga attagaaatt ttttgaccat
      121 agttaatagc ttctaatgaa cgaataacaa ccactgacgt gttagaaaaa taatccccaa
      181 tatgttgttt cttgggcgtg aaagcagcca ccaaataccc aatttgaaat acaaaatttt
      241 ttaatataaa cctacatata cactcacgaa taaagatagt ttgccaactt agctcttttt
      301 cctcaaagct cactactctt atcccaaaaa tcattttccc aattgtttgt cctgaattca
      361 gcttagtcaa aagtacaaaa taggttaaat aaatagctaa tgacgctata ccataaaaag
      421 taataatgga tttgtcagat tccagtccca tgattttgta aaagagacct atagtagcat
      481 gggtgatgat gctaatacaa attaaatcaa taagaaaagc aaaaaatcgt aaccaaaatc
      541 ccgcaaatac ataattaggc aaagtttcaa tgccactctt cttattcatt ttatagccct
      601 ccatagtaat acattggttt tggagtatca gaaaatccaa gtttttcaaa aactgaaatt
      661 acacgatctg atgtagttgc ctttagccct tgccattcag caatcttatt acctaaccaa
      721 gaagaagcaa agccagttgt gtcattttta tattctataa ccgtcgcatc agttaatttc
      781 ttctcttttt ttagggaatc taatgcatct tcagagtaac caatttgatc caccaaccca
      841 ttttctactg cttggttacc atcataaatt cttccatccg caagttcttt cactgcatta
      901 gtatccatat ttcttccttt tgctacaaca ttgacaaaac gatcataagc actcatcaca
      961 aattcctgta taactttatt ctcttcctct gaaggtttgt gttgaggtga catcatatct
     1021 tttaatgcac cacttttata cgtcgcatca gttacgccaa gtttttctaa taagccagaa
     1081 taatttaaac cagaaataat gacaccaatt gatccagttg ttgtttcttc cgtagcaaat
     1141 attttatctg aaccagcaga aatgtagtac cctccacttg ccgctgtatt tttaaatgca
     1201 gtatagatag gaatatctag ctttttaatt tttgccattt ctttagcaat ctcagcactt
     1261 tcatatattc ctcccccagg tgaatttact tctaataaca ctcccttaac cgctttgtct
     1321 tcttgaattt tctttagctg tgttaaaaaa ttctgatggt tatattgttc cctactaaaa
     1381 agattgcttt cacctgtatc tgcaataact ccattgacgg atagtttgac aatcttttta
     1441 tttgatgcgc cttcttctaa cgtttcttct gtgatttcgt tagatccgta aaaaatctta
     1501 tttattcctg ttagggttgt atttgatgct tcctctttat cttttcctgg aattagcgcg
     1561 acgataatgg atagtaacaa taatccgcac gctactccca cagcaatcca tcttctttta
     1621 ttcatataaa ctcctcctat attcttagta aaaataatta ttttttattt agaaacacat
     1681 cgacaatttg ttgccaatat gttttttctc ctacaattaa agacaactct ccagttaatc
     1741 cataacgaaa cggaaacgat tgagttgttt ttaacgttcc tttcactgca taaaaaacac
     1801 ctttctcagt ggcagtagaa ttttctgaaa tttcatctaa agttccgtcg atcgtaatcg
     1861 gagccccacc tttttgatcc aatttaaaat gaaccttcat cccttcttta atataggtac
     1921 tttcattcgc aggtaaaagt gctgtaaatt tcatttttga tttttcgtgg atcggataaa
     1981 tttctgcaag cactgttcct ttggctagtt ctgcttgtcc ctttgtttct tcatttaaat
     2041 gaaccgttcc agatacagga gcttttagta ttccttttga aagttgatcg tccaaacttt
     2101 tgataactga ctggttcttc tcttgttttt catttagctc tgtaactgtt tgttttacag
     2161 ttgttattgc ttgttctagc tcttgctttc ctttttccgt ttgactaatt ctgtcattct
     2221 cagaagtagt tggtttaact aatttagctt tttctgactg atactgttct acttcttttt
     2281 ttagttgctc aatttgttca tttatgcttg ctgaaattgt cagtttcact tgattttttt
     2341 gatcatcaga aacattattt agttgttctt gccaattctc atagtttgcc atcatttctt
     2401 ttgaaaaatc ttttaatgat tgattattac tccaagctac ctgtacttgt tgccattctt
     2461 gtaatttact atctgaactc tcaatttgtt tttctagttg tctttttgtc ttttcgtata
     2521 cttctaattg cttttgatca tttaattcac tttgcttcaa agcgtagcgg agtgattctt
     2581 tttctgacaa catgctcttt aattgattac tgtagccaaa actgtcatcc gttgaaaaca
     2641 aattttttcc tttacttata ctttcaataa atagctgagc catctttatt tgttgagtaa
     2701 tacgttggtt ttcttgttct atctgagcct tttcattctc tattaaagaa caatccaatc
     2761 taaccaacac atcgtcctta tgaacaaatt gattttcttt taggaagttt tccattattt
     2821 tagcttctaa tggcacttgt aacttctcta ttttttcggg gttcaattga gccgttgtcc
     2881 gaataacaac ctctttttta gcaaaggtta aaaacaatcc aataatacat aaaaaaagaa
     2941 ttacagggta taacacccaa cggtagaagc tatgatgttg tttagtatag atactagaat
     3001 gttcgaacca attattattc tgcataaatt gtttccctct cctagatttc ccataatttt
     3061 tgatagatac catcggcaaa acgaagttca tcgtgcgttc ctttttctac caaatgccct
     3121 tcatggagaa ctaaaatttg atcacatgct tttgctattg gtaaatgatg cgcaataaaa
     3181 ataatcgttt tttcttttaa attgaggaga taattcataa ttgcatgttc taacaaggta
     3241 tctaatccac tagttgcttc atctaaaatt aatatgtccg gattttttaa taaagcccgt
     3301 gctaaggcta atcgttgtcg ttgccctcct gaaagattat ttccaccttc ttccaacaat
     3361 gtctcgtaac gtaatggctg ttgattaata aattcattta gttggactat ctcacaaact
     3421 tctattattt gttcgaaact tggtgggcta tctaaaccga atataagatt ctcaatgatt
     3481 gttccactaa agaaaaatga ctcttgaggc aaatatgtca ctcgatcgcg taatatctga
     3541 aaaggaatgt ctaagtgatt aatgtttcca taatggatcg atccttctga tgcttcataa
     3601 aaacgaacaa ataactttgc aagagttgat tttccagaac cactaactcc aactatagcc
     3661 gttttactgt gcgaaggaat tgtacatgaa atttcgttca acgttggggc cttcatgcta
     3721 tacgagaatg aaacgttttt tacagtgata tcttgttgaa ataattgttc agaaagcata
     3781 tattcaaaag actggttcga tttttctgaa tcaatcgcga aaatttcatt taaacgatta
     3841 tttgcaactt gagccgtttg cattttcact tgtaaattaa taatgttttg taaaggatct
     3901 gtcaaaaata ctagtaaagc attgtatgtg ataagttgac ctagactaat tctattatcc
     3961 ataacaaaat aagaacctag ccataaaatt aatgcactgc taattaattg aatcaaatgt
     4021 ttaattcctt gttgaatatt atcaagcgta accgtacgaa atgatttttt cattaattta
     4081 acaaattcat ggtctatacg gttatatact ttctcttcac cgctatatgc tttaattgtt
     4141 tctattcctt taagactttc tatgatacta gaatttacag ctgcaccggc tgccatttct
     4201 tccctgttcg atttttcata gcttttcacg aaagcgaata ttgcaactac ataaaaaggc
     4261 aaagagcata gagtgattaa aaacaacgta ctattctgaa tagctaacgt aaccccaaca
     4321 agcaacacca taccaatatc taaaaatatt gataacgtag cacttgctaa agcatcaata
     4381 atcttactgg catcaagaaa acgagaaata atttcccctg attttctcgt agcaaaaaaa
     4441 ttcattggta aagttaacac atgtttgaaa tactgaagca taattgccat actcattcgc
     4501 tgtcctaaaa taataagaag ataactacga ctatattcaa acaatacccg aaataaatag
     4561 acagcgatca agccaatgga aattatattt aaagttgaac gtgcttgatt agggatgaaa
     4621 aaatcaagaa taccttgaaa ataatatgag cttcctatgc caaaaaacgt aatgaaaatc
     4681 gatgctaaaa cgatgtggaa tactaatcct ttttgtttcc aaattatagg caaaaaagaa
     4741 cttaatccag ctactttttc tttaatagga atataagttt ctctaggcat aggtaataaa
     4801 agaatacctg tccattcttt ttgaaacgct tcaatcgttt ttctattttt tccattagcg
     4861 ggatctgcaa tccatagtac gtttcccttc gttttataaa ttacaacgta gtgcatatag
     4921 ctgttatcaa ccaatatatg tgcaattaat ggtaagataa gatctctttc tttccagatc
     4981 tcactatctg cttgaatagc catacaatca aaagacaatt tttcaaatgt ctttttcaat
     5041 ccaaatgctg aagtaccctc taaatcagtc ccagataggt cccgtaattt atgaatcggg
     5101 atatcagttt taaaatattt taaaatcatc gaaaggcaag caacaccaca atctttttca
     5161 tcttgttgct gcacaaaagg aaatgatttt atcacttatc taacacaact ccttctaaaa
     5221 aatttttata gaaaaattct caatatagct ttaatacagt gaaaatattg tctcataatt
     5281 ccacaaaaac tttgaaaaca gttcttgaac actaactttt tcattcctga atcaaattta
     5341 ttttggaaat tttactattc aagaataaac ttgataagtt tcaggaagat ttttactgtt
     5401 aatcaaatac gtatgttaca gtagcaccgt aaaaaatatt atttaaagaa gcgtgtttaa
     5461 acatgcaaaa tgtaaaggaa ctaagtattg tggaaatgca caaactgttg gtggaggcaa
     5521 taatgcttgg ggaaaattgg ggcaagttgt aggtggactt accacagggg cagtaggtgg
     5581 tgcaggttta ggaacagcaa tttgcggacc agcttgcggt gttgttggag ggttatatgg
     5641 agctgtcgct ggaggtgctg cagctggctg ggatgctcga aaaaaataaa aaaggagaat
     5701 aagtagtatg catattaaaa acacaaaaac aacatttata ttatcttcag aagagttgaa
     5761 aaatattcaa ggtgggagtg ccgtgggagt tttaggcact actttttctg gggcaacagc
     5821 aggagtaaaa ctttgttcag ccggaggacc ttatgcaata gctgcttgtg gtgtaggtgg
     5881 agctttatta ggtgctggat tcagcatgtg gaccggtgcg taaacaatta aaaaatatca
     5941 aacctcaaga ttggtttgtg tcgattacga acataggtat gttctttatc ttttcaaaag
     6001 tttggaatgg acagaacttt ttactaaagc ttcttgtgct agtttttata attatattaa
     6061 a
//
LOCUS       DAZUKD010000148         5902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000148 DAZUKD010000000
VERSION     DAZUKD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.151"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..599)
                     /locus_tag="ACG8N9_002596"
     CDS             complement(114..599)
                     /locus_tag="ACG8N9_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898140.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(738..953)
                     /locus_tag="ACG8N9_002597"
     CDS             complement(738..953)
                     /locus_tag="ACG8N9_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4898141.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1177..1977
                     /locus_tag="ACG8N9_002598"
     CDS             1177..1977
                     /locus_tag="ACG8N9_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4898142.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1996..3864
                     /locus_tag="ACG8N9_002599"
     CDS             1996..3864
                     /locus_tag="ACG8N9_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4898143.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3857..4348
                     /locus_tag="ACG8N9_002600"
     CDS             3857..4348
                     /locus_tag="ACG8N9_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4898144.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4336..4890
                     /locus_tag="ACG8N9_002601"
     CDS             4336..4890
                     /locus_tag="ACG8N9_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4898145.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4908..>5902
                     /locus_tag="ACG8N9_002602"
     CDS             4908..>5902
                     /locus_tag="ACG8N9_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4898146.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2061 a          944 c         1190 g         1707 t
ORIGIN      
        1 taacgaatta ttaggttaat gcaaatgctt atttatactc cgtataactt tctcagaaaa
       61 aatgatacca ataatgactt gctagtactg taaatccgta taatatcatc taatcaaaat
      121 ggtgttccat ttgcatctgt catcgtaatc gatccttcgg gtgacactcg ataaaaacca
      181 acgcttccag atcctccttg ggaacggatt tgtttgctgg aagctgtgaa caaataatct
      241 cgatcaattt tttgataaaa gctaataaca atatcgtcac tttgtaattc ggaacggtgg
      301 gcaataagaa atagaagtgc tttgtcagag ttttctaatg gaagagaagg tacagtgctt
      361 tcttgcattt cagatgttga agaaatgcta aacgactgaa tacttgattg gctatgttgc
      421 gaatctacat taccagtaga agtattagta atggaagtag tagaagtact tttagttgaa
      481 ccaacctcag ttgttgaaat ggctgctgaa gattctttaa cagaattctt tgttttatat
      541 ccacaggcag aaaaaagtat tacagttccc aacaagcctg cgtaaatccc tttatgcatg
      601 atagctcctc ctaaaataga ttatctttat tcagtatatc acttaaacaa agaaaagcag
      661 atggtttaaa atgaaaaaac cttcccaata aagggaaggt tcatgaagat aataactttt
      721 ttaataatta tagctttcta atatctttca tcaacttttc tagtaatcat tgggaaaaat
      781 ttcaagcagt agatcgcaca aatacctacg acgatataaa tgatcttcgc aaaaatagtt
      841 gttgaaccac cagcaatcat agctactaaa tcaagttcga acaagcctac caacagccag
      901 ttcaaccctc ccacaatcaa taaagttaat gcaatagcat ctaaagtttt cattagaatt
      961 ctcctttctt aaatgataaa acaattatat atcaaaaaga gaaacattgc ttatataacg
     1021 ctcaggaatt ttagcaatct atgcaaaaag atgaaaattt tttttgctct tccattagta
     1081 cacatttttt agctgaagct gtaaatttca tgtcttaatt ttttttagtg agcttttata
     1141 ctggatttgt aagaaaaaag aaggagagga tcacatatgt cggattggtt ggatattgca
     1201 ggaaaagtag taattgtaac tggaggttcc tcaggaattg gaagaagtat cgtcgaaaat
     1261 ttgttgaagc agaatgcaca agtagcgaat tttgatgtaa ctgaatgccg tatacagcat
     1321 gaaaacctac tatctttaaa agtcgatgtg tcttctaaaa cggacataga agaagggata
     1381 tataaagtaa tgaaacattt caagacgatc gatggtctag tcaataatgc aggaataaat
     1441 attccttctc ttctaataga tagaaatcat ccaaaaagca agtatgaatt atcagaacaa
     1501 gtttttgata agatgatcgc tgtcaatcaa aaaagtgtat atttgatgag ccaagcagtt
     1561 ggaagaatct tggtgcaaaa gggatcagga gtgatcgtga atctttcttc tgaatcagga
     1621 ttggaaggat cagaaggaca gagctgttat gctgctacaa aagcagcgat gaacagtttt
     1681 actcgttctt gggcaaaaga gttaggaaag aggaatgttc gtgtggtagg tgttgcacca
     1741 gggatactgg aagaaaccgg tttgagaaca caagaatatg aagaagctct ttcttatacg
     1801 agaggtataa gcgtcgaaca gctgagaaac ggttacagtc gaacaagtac gatccctcta
     1861 ggaaggagtg gtaagctgca agaagtggcg gatctagtct gctattatct ttcagaaaga
     1921 tcaagttata tcactggagt cacgactaat atttcaggag gtaaaacaag aggataaagg
     1981 atagggggga cgcacgtgac gctagtaaac agatggtacc aattattgaa attactagtc
     2041 actcacaaga aagtttcaat ccaggaagtg caaaaagaaa tcaaaaccag taatcaaacc
     2101 ataaaaaaag atattgggga gttgaatgaa gaaattgccg gtattgcaaa aatcatccaa
     2161 aaaaacaaac attttgaact tgaaatttat gattttgatt tttttgatga gatcatgcaa
     2221 ggaaaattga aaacagccag tgatttcaat tcggcatcca aaagaatcgc ttatttacta
     2281 aataggctga tcgaaacaga ggatttctta cgtatggatg atttgtctga agaagtcggt
     2341 gtcagcagag gaacagtggt caaggatcta aagacattaa aagagatgat caatgatttt
     2401 gatgtaaaga ttacaggcac accaaataaa ggactaagaa taataggaga tgagctagaa
     2461 ttacgactac ttttatttta ttttgtttct ccctactttt cagagacgaa aacagaacag
     2521 tttctagaag aaaaacaagc aatcaaacat tttcagaaga agacgaatgc tagtaaacaa
     2581 gaaatcaccc ttttaacgaa agttatcggt atttctttga atagaatctc tagtaactat
     2641 ctgttgaaga agcccatcaa tcgttacagt gggtgttttg aatatatcca agaattcaat
     2701 gaactgatca atcagttgga agaaatctat gaactaactt tgagccaata tgaaaaagat
     2761 tttatcagtt ttcctttgaa catcatcaac acaagtataa cgatgataag gaaagctaac
     2821 tctgatttgc gcttatattt cgaccccatg atgatgcgta ttcgacgatt agatctcatt
     2881 gatctggatg agtccttctt atatcaagag atgaaacagc acctctcttt attgataaat
     2941 cgggtaattt ttcgttatcg attgactgat ttattttatg gtgagataga aaaaaagtat
     3001 cctttttctt accaaatcgc tagcgaatgc atacaagcat tagaagctat cttgcaacat
     3061 aagatctcta aagtagagat cagctaccta acgttgtatt tggagttagc atttcgatat
     3121 caaaaagagc atttttcctc taaaaaagta gccattgttc atacgacagg aaaaggaaca
     3181 gcattaatga tccaaagaca aatcaaaagg atattgggga gggatataca aataacacat
     3241 gtttcagaag aacaatatga aaaagtagat ctaaacaaat atttcgctgt ttttacaacg
     3301 attccgttaa aaaatgtaaa tgaagagaca ccggtgatcc atttgactaa tctttttgat
     3361 gactcttggt taagggcaca atggcaaaaa gcaaaaaaaa agcgattagc cgggtttcat
     3421 tctactgttt ttttctatca aatatggaaa gaaaaaaaga cgtatcaaga atatttaaac
     3481 aaattagtcg ctcctttgat cagaggctat tatgtggatg aagaatttga aaaaaggatc
     3541 ttgaaaagag aatatcagca aagcacattt gttaacaaag aatttgcttt tcctcacaca
     3601 accaaccagc tatctcaaca cattgtttta tcagtagggc taataactga tcaaggttta
     3661 gcagaaacag gaggaaatgg agttattttt ttatttgcca tacctgaaga aatgacggct
     3721 gaagttgaga atgaattatt tgagctatac gatatcctat ttgaactcat aaaaatggga
     3781 atagatgagt ttattgaatt agcaccgaca caagaatcat ttttaaaact aatcaaaagt
     3841 aaaggagaga gacttcatga atgatatgta tctattcata gttttcgcag cttcagctta
     3901 tgtgctgcaa gcagttatca gttggaagca actaaagcat ttcaatcaaa catatagcga
     3961 actgcgaaga aaaggaaaag tagcgattgg taaacgacct gggaaaatcc gttcaggcac
     4021 gattgttatg ctagctatag acgatcatga tcaagtttta gaagtcagaa aaatgcaagg
     4081 ggttacatct ttggcaaaat ttcattcatt acctgtctat gcgggtgaag atatccatta
     4141 catcgatcgt taccatccct cagttcgagc ggaagatcgt ttgacacaag cagctttaga
     4201 aaatgccaga gaagtatact tacgaataga ggcaggaaat gaaaaaagtg aacaaccttc
     4261 attgcctttt attgctatgc tcaataattt tcggatatgg aaaagtacga tcaaagaaaa
     4321 aaggagtgac aaacgatgga tatcataaca aaatttgctg agggatttat gaggctgttc
     4381 caaacaggtg cagaaacatt cataagctgg atgacaggaa tcgtaccagt tgtcttgatg
     4441 ctgatggttg caatgaatac gctgattgct tttttaggtg aagaccgagt aacgaaagta
     4501 gccaaattat cagcgaaaaa tccgcttact cgttaccttg tgttaccgtt cttatctgct
     4561 tttatgttgg gaaatcctat gtcatttaca atggctcgtt tccttccaga atactacaaa
     4621 ccaagctatt atgctgctca agctcaattt tgtcatacga gcaatggtgt attcccccat
     4681 attaatcctg gagaattgtt tgtatggatg gggattgctc aagggatcga aaccttaggg
     4741 ctaaattcga tggagctggc tatccgatac ttgcttgtgg gactgctgat gaactttatt
     4801 ggtggttgga taactgattt tactactgga tttgtatgca gacagcaagg aatcgttctt
     4861 agcaaaaaag tagagctttc agttgattag agaaaggaga gttttaaatg tatggtgata
     4921 ttaagatcgt aagaggaaac ggcgggtatg gaggtccttt gattattagg ccgacaaaag
     4981 ataaacacaa gttcatctat gtcacaggtg gaggtgagag gccaatgatc gtagataaaa
     5041 tagaagaact gacaggtatg gagtctatca atgggttcaa aacatcaata cctgatagtg
     5101 aaatcgcgct tgcaatcgtt gactgtggag ggactcttag atgtggaatt tatcctaaaa
     5161 aagggatacc aaccatcaat atcgtttcca caggaaaaag tggcccatta gcacaatata
     5221 tcactgaaga tatttatgtt tcagcagtag ggttagaaca aatacagcca ctaatggaaa
     5281 ctgatgataa caaagaaaca gtaaatactg catctccaga taaaaagtac aaatatgata
     5341 cttctaaaaa gataaccgag caaaaagcag agcagcaaaa ctggatagca aaaattggaa
     5401 tgggcgcggg aaaagtagtt gctacattca atcaatcagc aagagaagca gtagatacga
     5461 tgatccggac agtgatccct tttatggctt tcgtttccct tttgattggt gtgatacaag
     5521 gttctgggat cggtaatgta tttgctaaca ttatggcacc tttagcagga aatgcagttg
     5581 gattaacaat cattggcttt atttgttcac tgccattttt atctcctttg cttggtccag
     5641 gggcagttat tagccaagtg attggaacat tgatcggtgt agagattggg aaaggaaata
     5701 ttcctcccca attagcatta ccggcactat ttgctatcaa tactcaaaat gcatgtgatt
     5761 ttattccagt cggtctaggt ttggaagaag caaaagctga gacagtagaa gtaggtgtac
     5821 catcagttct ttattcaaga tttcttaacg gcatcccacg agttatcatt gcttgggtag
     5881 caagttttgg attatatagc tg
//
LOCUS       DAZUKD010000149         5749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000149 DAZUKD010000000
VERSION     DAZUKD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.152"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8N9_002603"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8N9_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4898147.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKML
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG8N9_002604"
     CDS             complement(1164..1874)
                     /locus_tag="ACG8N9_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4898148.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="ACG8N9_002605"
     CDS             complement(1942..2757)
                     /locus_tag="ACG8N9_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4898149.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG8N9_002606"
     CDS             complement(2768..3736)
                     /locus_tag="ACG8N9_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4898150.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="ACG8N9_002607"
     CDS             4499..>5749
                     /locus_tag="ACG8N9_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4898151.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1184 c          916 g         1877 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagcat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatact tgcttcataa ataaaatcta
     1141 ctccttgaaa tcaaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 acatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctattttttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgtg
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DAZUKD010000150         5717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000150 DAZUKD010000000
VERSION     DAZUKD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.153"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG8N9_002608"
     CDS             196..921
                     /locus_tag="ACG8N9_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898152.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG8N9_002609"
     CDS             911..1420
                     /locus_tag="ACG8N9_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898153.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG8N9_002610"
     CDS             complement(1490..2938)
                     /locus_tag="ACG8N9_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4898154.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG8N9_002611"
     CDS             complement(2938..3654)
                     /locus_tag="ACG8N9_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4898155.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG8N9_002612"
     CDS             complement(3635..3985)
                     /locus_tag="ACG8N9_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4898156.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG8N9_002613"
     CDS             4129..4902
                     /locus_tag="ACG8N9_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4898157.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8N9_002614"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8N9_002614"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5485..>5717)
                     /locus_tag="ACG8N9_002615"
     CDS             complement(5485..>5717)
                     /locus_tag="ACG8N9_002615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898158.1"
                     /translation="FEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLG
                     ISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1660 a         1124 c          993 g         1940 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaata
//
LOCUS       DAZUKD010000151         5494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000151 DAZUKD010000000
VERSION     DAZUKD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.155"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..460)
                     /locus_tag="ACG8N9_002616"
     CDS             complement(<1..460)
                     /locus_tag="ACG8N9_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4898159.1"
                     /translation="MRCQQHLTYVFLLKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACG8N9_002617"
     CDS             complement(500..1552)
                     /locus_tag="ACG8N9_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4898160.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACG8N9_002618"
     CDS             complement(1565..2503)
                     /locus_tag="ACG8N9_002618"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4898161.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2954..3604)
                     /locus_tag="ACG8N9_002619"
     CDS             complement(2954..3604)
                     /locus_tag="ACG8N9_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898162.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3704..4213)
                     /locus_tag="ACG8N9_002620"
     CDS             complement(3704..4213)
                     /locus_tag="ACG8N9_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4898163.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4576..4959)
                     /locus_tag="ACG8N9_002621"
     CDS             complement(4576..4959)
                     /locus_tag="ACG8N9_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4898164.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            complement(5200..>5494)
                     /locus_tag="ACG8N9_002622"
     CDS             complement(5200..>5494)
                     /locus_tag="ACG8N9_002622"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898165.1"
                     /translation="TNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERR
                     EQGKEVFLFTDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         1734 a         1131 c          802 g         1827 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttagcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     3061 tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     3121 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta ttgagttgat
     3181 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     3241 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcacttca cctaatgaat
     3301 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3361 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     3421 tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat
     3481 tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc
     3541 tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat
     3601 tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc
     3661 tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat
     3721 tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat
     3781 ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagagttctg
     3841 cctcagcaaa aacttctaaa atatcatcag tggtcataga caaggcgatt tttaattttt
     3901 ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg
     3961 gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa
     4021 cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct
     4081 gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa
     4141 aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat
     4201 cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt
     4261 cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgatac ggagctgggt
     4321 atcatgagtg aatgatattt ataaatcact ggatattgaa gtatattctg ttttctcaac
     4381 ttatttagtc agatttttta atgaagattt tttgagtaaa atttctataa ttcctactta
     4441 ttgaaaagta gtttgtagtg ctgcttctac ctctctcatc tgttctacaa attccaaata
     4501 acttgacgca gcttgcggaa tgaaaaaatg atatgtgaat atttctcctc ctagcccgct
     4561 taatatgcct attttttata cccatgattt gcaggaaggg gatcggaagt aggttgcaga
     4621 aagccgcctt tcataatctg tcggaaaccg gacatcaaaa cggattattt tacttgtcac
     4681 atttgtattt tcttgtgagg aagtgccaaa tacattattt acttgagtga cattctcatt
     4741 gtcctgtctg atattgcttc gtataaaagt accagatcca cgtctcgtat ataacaaacc
     4801 ttcatcaata aggagttgta tagctttgcg gatagtaaca cggcttgttt taaactctct
     4861 agccagactt tcttgctcag gtaatttaga accttcctga tattttccct caacgatcct
     4921 ctcttttaga gccaaggcta tttctttata ttttggcata ttttcccctc atttcattga
     4981 tcctacaaaa aaattagtat atatacaaat ttttgtaaag tatatatact aataaataca
     5041 aaaatatttt tattgttgga tacagaaatc tatttttaac tggggatatc tttgccagct
     5101 aatatctttg agactacacc tatcttcatc taaatggatc aattactcgc tttttccatt
     5161 gaccttcgtg gtacttctgc tttctgatag taaaaaacat tactgaatga tcttactata
     5221 taacatacag cggagatcta cttctttatt aaggatcttc atgacgttat ctttttcttt
     5281 tttccgttcg aaaccgcgtc gttcataaaa ttgatacgta cagccgctat ccgtgaacag
     5341 aaatacttct tttccttgct ctcggcgttc aaattctttt aataatttac taccgatccc
     5401 cttgatcttt aaatcagggt tggcagctaa aaagacaatt tctccatctg gggaatgttt
     5461 ttttaaatag tctgaaagca attcgtcatt ggtg
//
LOCUS       DAZUKD010000152         5385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000152 DAZUKD010000000
VERSION     DAZUKD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.156"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..1111
                     /locus_tag="ACG8N9_002623"
     CDS             236..1111
                     /locus_tag="ACG8N9_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4898166.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1215..1979
                     /locus_tag="ACG8N9_002624"
     CDS             1215..1979
                     /locus_tag="ACG8N9_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4898167.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1966..3408
                     /locus_tag="ACG8N9_002625"
     CDS             1966..3408
                     /locus_tag="ACG8N9_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4898168.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3419..4749
                     /locus_tag="ACG8N9_002626"
                     /pseudo
     CDS             3419..4749
                     /locus_tag="ACG8N9_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4762..5220
                     /locus_tag="ACG8N9_002627"
     CDS             4762..5220
                     /locus_tag="ACG8N9_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4898169.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1822 a          779 c         1121 g         1663 t
ORIGIN      
        1 tttcttatag agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc
       61 gatacaagta ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt
      121 aaactatttt tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg
      181 gttctttctt aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa
      241 ggagtctgca agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat
      301 gaaaaagctg aatcataatg ctgtattgat cgatgacgat ggaatagaaa aaattgcgat
      361 ggggaaaggg attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga
      421 caaattattt gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca
      481 gattccaatc gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat
      541 ctcctctcca ttagattcaa atatctatat tacattgaca gatcatattt attttgctat
      601 tcaacgaaaa agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct
      661 ttatccaaat gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca
      721 aaccgagtta gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct
      781 aggagagcct aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc
      841 ccatctagaa aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt
      901 aatggttcat atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga
      961 aaatattggt ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa
     1021 gattaaagat atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta
     1081 tttggcaatt catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa
     1141 ccaaatgaca catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa
     1201 agaaggagaa tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg
     1261 gtgtgaatta tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga
     1321 tggcaaatat gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag
     1381 gtgtaggagt ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc
     1441 ttatcgaaag cggagagttc agatccttat cttattatca gcatccattt aacattgaca
     1501 aagatcaact gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca
     1561 taccaactca tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag
     1621 ttgttacatt agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg
     1681 aagttcgtca tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa
     1741 aaaaacgaca aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga
     1801 gagaagataa aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa
     1861 atggttcaag ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat
     1921 ttgctcaaaa agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat
     1981 agaggaggaa taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta
     2041 cttccagttg cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg
     2101 ctgaacatcc cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta
     2161 tttgctatag gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt
     2221 actggttttt tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg
     2281 aatatgggga tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc
     2341 tttaaagaac aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg
     2401 actatggtga tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc
     2461 atccaaattg ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg
     2521 gggatattta tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat
     2581 acgtatattt attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga
     2641 gagatgacac gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt
     2701 gtagtaatga tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa
     2761 gaaaataaag agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca
     2821 aatatcactg agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc
     2881 catgcacttt ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt
     2941 tttacattcg gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct
     3001 attttgattc ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat
     3061 ttgatcaata agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat
     3121 gaagtaacct tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca
     3181 acaaaaatgc tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat
     3241 acacgcctac gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa
     3301 ttagggataa gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta
     3361 gaagtgactt atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat
     3421 gatgatgaaa aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat
     3481 cgttcaagcc attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga
     3541 tattgatgaa gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa
     3601 ggtttttcaa atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc
     3661 gattttgttt tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag
     3721 attccactta aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat
     3781 gggatgcgct caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc
     3841 aaagcatgga ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt
     3901 aaatatccga atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg
     3961 attgcagtga attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac
     4021 cactttggct ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag
     4081 attattgaaa aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa
     4141 acttggcaaa aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc
     4201 ccgaataatt acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa
     4261 gagtatacac gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct
     4321 gataaaatac gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga
     4381 caatatatcg tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta
     4441 atcgttccta atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg
     4501 gcatatgttg gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt
     4561 cataaaggat tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt
     4621 gaaggttctt atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc
     4681 agagtcgcaa aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca
     4741 ttaaagtaga ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta
     4801 gctagtggtc aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg
     4861 ataggagttg gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga
     4921 ataatcgata atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga
     4981 aaaatactga tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata
     5041 cgggttgaaa gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag
     5101 aagttggagc aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa
     5161 ggaaatttat taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag
     5221 tgtacgatga aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg
     5281 aaatgataag tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt
     5341 tttgttgctc ctatgattta gaaaacttat tttcaggatt cttaa
//
LOCUS       DAZUKD010000153         5343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000153 DAZUKD010000000
VERSION     DAZUKD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.157"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8N9_002628"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8N9_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8N9_002629"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8N9_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="ACG8N9_002630"
     CDS             2092..3132
                     /locus_tag="ACG8N9_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4898170.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /gene="traE"
                     /locus_tag="ACG8N9_002631"
     CDS             3956..4288
                     /gene="traE"
                     /locus_tag="ACG8N9_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4898171.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="ACG8N9_002632"
     CDS             4907..5248
                     /locus_tag="ACG8N9_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4898172.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1944 a          728 c          899 g         1772 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta taacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 ttt
//
LOCUS       DAZUKD010000154         5246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000154 DAZUKD010000000
VERSION     DAZUKD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.158"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..655
                     /locus_tag="ACG8N9_002633"
     CDS             <1..655
                     /locus_tag="ACG8N9_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4898173.1"
                     /translation="EFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGIRKGEAFALTW
                     SDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLLRKYYTKNKTI
                     VTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHTHASLLFESGA
                     SLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGESKGESKNILG"
     gene            complement(707..794)
                     /locus_tag="ACG8N9_002634"
     tRNA            complement(707..794)
                     /locus_tag="ACG8N9_002634"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Ser,seq:cga)
     gene            complement(939..1505)
                     /locus_tag="ACG8N9_002635"
     CDS             complement(939..1505)
                     /locus_tag="ACG8N9_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4898174.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1760..3091
                     /locus_tag="ACG8N9_002636"
     CDS             1760..3091
                     /locus_tag="ACG8N9_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS4898175.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3057..3407
                     /locus_tag="ACG8N9_002637"
     CDS             3057..3407
                     /locus_tag="ACG8N9_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898176.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(3499..3918)
                     /locus_tag="ACG8N9_002638"
     CDS             complement(3499..3918)
                     /locus_tag="ACG8N9_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4898177.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(3919..4263)
                     /locus_tag="ACG8N9_002639"
     CDS             complement(3919..4263)
                     /locus_tag="ACG8N9_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4898178.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4613..5209
                     /locus_tag="ACG8N9_002640"
     CDS             4613..5209
                     /locus_tag="ACG8N9_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4898179.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1493 a          954 c          904 g         1895 t
ORIGIN      
        1 cgaattttat actaaagaag aattaatcaa atttttagaa gcaataagaa acgatgatga
       61 tgaaagatat ttattctttt ccctattggc ttttacaggt ataagaaaag gagaagcttt
      121 tgctctgacc tggtcggata ttgactttaa gagcaaaaca ctcaatatca ataaaactgt
      181 aacaagaggt tatcaaggaa gattgatcgt aaatactcct aaatcaaaat ctggaaaaag
      241 aaaaatttat ctggacaatg atttgatcag tttattgaga aagtattata caaaaaacaa
      301 aacgatcgtt acaatccaaa gggaaaactt aatttttcac catgatggtc ttctctataa
      361 ccctacagtt tcacgttcgt ggcttaatgt catatataag catcatccag aattgactaa
      421 aagaatcact actcatggtt ttcgacatac tcatgcctct ctcctttttg aatcaggagc
      481 ttctttaaaa gatgttcagg aacgtttagg gcatgcagac attcaaacta cttcaaatat
      541 ttacactcat gtaactgaaa ctcaaaacaa aaaagtcatc aataattttg tagcatttat
      601 gaagaacagt actcaggggg agtcaaaagg ggagtcaaaa aacattttag gctaaatata
      661 caacaaaaaa gacacgatcg aaatcgtgtc taatcgttga tatataagga aggtacagga
      721 tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt gcattaccac
      781 tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc aagcctattt
      841 ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga cagtttgcag
      901 ccaacggact taccgatcaa attcatactg atagaacact atctgattat ttcaggatta
      961 tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg ttcttctgtt
     1021 ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac ccatgtacca
     1081 gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc ttctttaact
     1141 atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc atgttcatca
     1201 atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata gttcaaaccg
     1261 agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt tagttgttca
     1321 aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc cagataaatc
     1381 ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc tttaaaccct
     1441 tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata gacttttctt
     1501 ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt accctatttt
     1561 tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt tcagtcttct
     1621 ttaacttttt tttgcaaaat taaaaaaatt tggttaaatt caatgggttc ccttacattt
     1681 ttagttttga cctaaatgat agttgtccaa atctttcatt tcaagttata gttaaagaaa
     1741 atgaaaaaga aggtgtgtaa tggaaaacga aactgacgtt atttatattc atcctcaaaa
     1801 acgaatcgtt tcacagaaaa gaaagtattt ttatcttggt tttacaggtg ttttttttct
     1861 ttttattggc ttgctttcta atactcctac tgataattgg agtggattgc taactattct
     1921 tacctcgcca agcaatcttt tgacagatta ttttgcttta ggtgttttcg gaagcgcatt
     1981 cataaatgta gggattttga ccttactttc tgtgctttta gcttatcggc acaaggtgat
     2041 tttaaacggt cccttatttg cttcgattct aacagttacc ggtttctctt tttttggcaa
     2101 aaacctttat aattctatct caatcatttt aggtgtgtat ctatacgctg tatttgtcaa
     2161 taaacctttt tctcagtaca tcatgatcgg actgtttggt agcgtattat caccggtcgt
     2221 tagttatatc acttttggta tgcgttttcc tttactgata ggtattttat tggggaattt
     2281 agctggcata gcaattggta tacttcttcc gcccttagct gctcaaactt tagttttcca
     2341 tcgtggattc acactttata atattggatt cacctctggg ttgattgcga tgacttttac
     2401 tgctgttttg aggttattca gctattcaat cgtcgaaaat acattggttt tcaatgaata
     2461 tcactttcct ctcatttgga ttatttttgg ctttttttcg ctgacagtcg gaattggttt
     2521 ctattataat tcctttcggc tttcggggat tcgagaaatt ttcgattcat ctggaaaatt
     2581 gacaactgat ttcattgcta attctggtat tggtgctact ttgatcaaca tgggcttagt
     2641 tggtctgatg ctgtcttctt atgttttgct ggttggcggt cagttaaatg gacctgtcat
     2701 cggtgctatt ttatctgcgg tgggtttcag tgcttttggt tgtcatttaa aaaacagttt
     2761 tccgatacta gtcggaatat ttattgcttc tttgtttgga acttttcatg agatcaccag
     2821 tactggcatg cttgttgcag ctgtgtttgg gacaggtctt gcgcccataa gcggttttta
     2881 cggcagtttt tacggtgtaa tagctggtgt gctacatatt gctcttgtcc ataacgtcag
     2941 cactttgcac ggaggactaa atctatataa cagtggtttt tcaactggtt tcgttgcagg
     3001 tatccttgta ccgatactag ataactttac cgctgtcaga aaggaaaaaa agacacttgg
     3061 aaaaagaatt atcaaaaaga atcatcgatg agcttcttac ttatttatat tcccatgaaa
     3121 taactgaaat acgtattgga gtagaatttg ctgaggaagg gcttttcata gagatacagg
     3181 gtacggcaga aactcagcca gaagatttag atgtacttgc tgatttgctg aacaatcctc
     3241 gggaccctag tatggaaatc tactatcaag aactattagg taatccccga catgatcggg
     3301 aagattatca tctgttaggt agtttgattg atgacgtgga aatcatgtat gcccagccga
     3361 ttatcaatat taagatcttt cgacgtgctt ctcattccta ttattaaccc tattttctat
     3421 ttataaaaga ctgaatcaaa aaagcagctc ctgaaagttc gattaaaatc tactttcagg
     3481 agctgttcaa gcttgacatt attgattttc caatccttca tacacttcat ctgttacagg
     3541 ttctaaaaac tctgaattac cagcagtgat cgccaaatgt tcaaaccaac tgtcttttgt
     3601 cgcaccatgc caatgtttaa tccctttgtg cgttacgacg acatctccag ccattaatcg
     3661 tcttgctggc tttccttctt cctgatacca gccttctcct ccggtcacaa gtaagatttg
     3721 atagccgtct gtatggacat gccagtgatt gcgacaacct ggttcaaaag ttacattacc
     3781 tacaccaaca gaaacttcgg gatcattcac taacatattc agatagcttt gcccggtaaa
     3841 tacttgagcg taagcttcgt ttttttcacc tgctggaaaa atcacgccat tgtgtatttc
     3901 ttcgtgtttt gtcatcattt acgcctcttt cttatcttcg ctattttctt ctaaatagat
     3961 ttcttttgct aaactaaaag ctgaccaagc ttttggccat tctacataaa aagctaaatg
     4021 ggtaatgatt tctgctattt cttcactagt aatgccattt tgcttgccaa tcttcagatg
     4081 agctggcaat tgttcaaaat ttcctcctga aattaatgca gatatggtga tgatactacg
     4141 ctgatgagca gataattctt ctctggacca tacttgtcca aataaaacat catcatttaa
     4201 ttctgcaaat tttggtgcaa aatctcctaa ttgatctcgt cctgcggttt gtttttctgc
     4261 catttctctt attctccctt cacgactgtt aggtgattca actatggttc taaggcaact
     4321 ttattttcta ttgtttcttc gcatttcatt ttaaatgtag tctttcagcc tgtccaatgc
     4381 ttagtttatt cagtcgatat gctttttaag catagttgct aacgcttttt ttattttaaa
     4441 aaaaacaagt tattaaataa gtctttagtt ggatgttttt ttgagatcaa tcttttatac
     4501 ttatgatgaa cttacaaaat tgtttgtttt ctgtcatatc aaaaaatggc gcagttagtt
     4561 gcttttctag taaagtatag ttaacaaaga acctctaagg agttgatgaa taatgaatat
     4621 tgctgcctct cgaaaactga taaatttcat atccattatc ggattaggcc tatcaatcgc
     4681 gctgacgatt tattttatta atttaggtgt tttcaaagat ttgaatgcgt taagaggatt
     4741 agtaggtgat tccatcattt taggaccgat tatttttgtc ttcatccaaa tcctgcaagt
     4801 agttattcca atcattcctg gagggatcag tactgcagct ggtgtcctga tatttggccc
     4861 ttatgctgga tttatctata actatattgg aatctgcatt ggttcgatta tcatttttct
     4921 gctagggcgt cgttatggaa agccattcat actttccatg attagcgata agacatataa
     4981 caaatatatt ggctggctgg ataatcaaaa ccgttttgag aagctgtttg ctctagctat
     5041 ttttcttcca gttgctcctg acgatgcgtt atgtttgatg gctggattga caaacatgtc
     5101 agtgaaacga tatacactaa tcattttgat tgctaaacca ctgtcgattt tcttatatag
     5161 tatggccttg atttatggag gtcaatatct aagtggtctt cttggataat atttattttg
     5221 taaaattagt cattgattga caatgt
//
LOCUS       DAZUKD010000155         5194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000155 DAZUKD010000000
VERSION     DAZUKD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.159"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..754)
                     /locus_tag="ACG8N9_002641"
     CDS             complement(<1..754)
                     /locus_tag="ACG8N9_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4898180.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSE"
     gene            complement(966..1862)
                     /locus_tag="ACG8N9_002642"
     CDS             complement(966..1862)
                     /locus_tag="ACG8N9_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4898181.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(1855..2535)
                     /locus_tag="ACG8N9_002643"
     CDS             complement(1855..2535)
                     /locus_tag="ACG8N9_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4898182.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2536..3318)
                     /locus_tag="ACG8N9_002644"
     CDS             complement(2536..3318)
                     /locus_tag="ACG8N9_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4898183.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3311..4228)
                     /locus_tag="ACG8N9_002645"
     CDS             complement(3311..4228)
                     /locus_tag="ACG8N9_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4898184.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4392..4646)
                     /locus_tag="ACG8N9_002646"
     CDS             complement(4392..4646)
                     /locus_tag="ACG8N9_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898185.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1529 a         1098 c          873 g         1694 t
ORIGIN      
        1 cttcagaatt attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag
       61 caaggtggat agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca
      121 tgtatcgatt aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg
      181 cttcaccaat gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa
      241 agccacccca ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag
      301 ctttgatgcg cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt
      361 ttctaacatc tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa
      421 gattttgaat ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac
      481 ggtcctccgg agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt
      541 ctttttttcc aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca
      601 ttttttggat tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat
      661 ataattgttc tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta
      721 ttttcatttg ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta
      781 agtgtacaat tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt
      841 tctaatattc ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct
      901 tttttctcta ttaaatatat ataagataat acatttttct actagttctc actaatcttg
      961 tataattaaa ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg
     1021 gtcatctctc ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta
     1081 gattccgtat ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca
     1141 gaggtttgcg ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct
     1201 tttgaaaatg tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt
     1261 tgcagcactc gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca
     1321 gcgaacgcta cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct
     1381 tccacctctt tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt
     1441 cgagcaagat cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg
     1501 atcgttaaat acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa
     1561 ccgttaatag cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg
     1621 atgttttctt ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta
     1681 ttcagtgcat ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca
     1741 ccatattctt gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt
     1801 tgcaggaaga aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc
     1861 aaatctaaac cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt
     1921 ccctcgaaga ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg
     1981 ccaaactgtt tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag
     2041 cttcagcaga tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc
     2101 cctcgactct ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac
     2161 ttggacagaa gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc
     2221 aaatggtttc gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg
     2281 atcgcttttt gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa
     2341 caatgcttca cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata
     2401 tgtgcttaac aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg
     2461 ttcaagaacc tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt
     2521 ttccagcagg accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga
     2581 tgataccacg attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc
     2641 ttgttgcgca aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc
     2701 caagcctttg atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa
     2761 agaaagaagc aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga
     2821 taatgtccct aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata
     2881 gtttttgatt ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag
     2941 tcccgtgatc aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac
     3001 ataataagtc attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa
     3061 ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat
     3121 tttttctacc attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa
     3181 ttcattgccg tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac
     3241 gaccatcagt cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc
     3301 agttaataga ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt
     3361 tttgcccaac taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga
     3421 tggctgctac ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat
     3481 aatccctata accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa
     3541 tttttataaa ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt
     3601 gtccttcatg caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc
     3661 tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt
     3721 ctgccatccc tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat
     3781 tcgtgatcag tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg
     3841 ctttcttttt ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat
     3901 ccgacacacc gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat
     3961 aaagagcagg ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg
     4021 ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa
     4081 aggtagactt acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt
     4141 cgattgatac tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt
     4201 tcaagaccgt ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt
     4261 atagttgacg ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa
     4321 ttgaatgtca aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt
     4381 atacgcttgt atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc
     4441 tcttcactga aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt
     4501 ataaggataa ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt
     4561 tgcgttgtct tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
     4621 atggtaatcg ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc
     4681 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
     4741 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
     4801 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
     4861 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
     4921 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
     4981 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
     5041 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
     5101 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
     5161 aacgataatc gcaattaacg ttttgagaac tgtg
//
LOCUS       DAZUKD010000156         5067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000156 DAZUKD010000000
VERSION     DAZUKD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.160"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(773..1726)
                     /locus_tag="ACG8N9_002647"
     CDS             complement(773..1726)
                     /locus_tag="ACG8N9_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4898186.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1749..2504)
                     /locus_tag="ACG8N9_002648"
     CDS             complement(1749..2504)
                     /locus_tag="ACG8N9_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898187.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2501..3460)
                     /locus_tag="ACG8N9_002649"
     CDS             complement(2501..3460)
                     /locus_tag="ACG8N9_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4898188.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3453..4403)
                     /locus_tag="ACG8N9_002650"
     CDS             complement(3453..4403)
                     /locus_tag="ACG8N9_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4898189.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4553..5005
                     /locus_tag="ACG8N9_002651"
     CDS             4553..5005
                     /locus_tag="ACG8N9_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4898190.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
BASE COUNT         1666 a         1032 c          874 g         1495 t
ORIGIN      
        1 gagtataaaa tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag
       61 cttcccgccg atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca
      121 catgcctaaa tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt
      181 gaacattgtt tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg
      241 acagaaaata gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat
      301 ggaacatcgt ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg
      361 gacagaaaat agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga
      421 tggaacatcg tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat
      481 ctaagtttcg ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa
      541 tggatcacac tattttttgc cactccccat tctagaatca acccgtatga tgtagataat
      601 gaattcacgt tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata
      661 cgattttacg gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg
      721 taagaaacat tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa
      781 atgcttgatt cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat
      841 accagacatc tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta
      901 ccagttcatt ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt
      961 ctactacaaa taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac
     1021 cgtgagtgga tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga
     1081 aaccgaatct agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta
     1141 gtgctttttt gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt
     1201 cctctaattg agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat
     1261 tttgctggaa agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa
     1321 tttgacttag atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct
     1381 gaagctgatt aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt
     1441 tataggatga aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa
     1501 caccaagagc atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat
     1561 tatttggcac ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt
     1621 gtgtgctgct tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta
     1681 aaccaaatga tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa
     1741 tgttagtttt agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc
     1801 atttcgtata attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta
     1861 aataactgtc catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata
     1921 tcatgaagaa caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc
     1981 atgatttgaa ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa
     2041 tccgtgtctt gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga
     2101 gtgtcaatcg attgatccgc tatttcttct aacccaatgt gttccagtga ttcctgaacg
     2161 atttcctcgt cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact
     2221 aattctttca cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt
     2281 ttggctaatt cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct
     2341 gtatcttttg gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt
     2401 ggaccgataa aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact
     2461 gattttccat ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt
     2521 cgcgaatcaa ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg
     2581 ttgtattcca gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag
     2641 ctaccagaaa gcttccgata aatagctgac catgacgata agtagaaaag acacgataac
     2701 tgatatttgc gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc
     2761 ctgtcaaaag actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga
     2821 ggtttactgc ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata
     2881 acaggtacca gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat
     2941 tgccaaagct tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga
     3001 cttgtaaaaa ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta
     3061 ataaaaaaag attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc
     3121 cgaccatcag cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca
     3181 ctcctccgat aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca
     3241 atactgaagg agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc
     3301 tgacagctag cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa
     3361 aatgccagtt tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa
     3421 caagcatacc aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga
     3481 agataaagga agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa
     3541 ggaaaaatca acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa
     3601 gcagttacgc caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga
     3661 atgatcactc cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat
     3721 gatactaagc caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca
     3781 aacgattcgc ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac
     3841 aatggaacaa cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct
     3901 tgaagccatg aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact
     3961 gaaccaatga tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg
     4021 aggaacaatc gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct
     4081 gtcaaggatc ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct
     4141 aaacgcgcac tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa
     4201 tgttgcatga tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt
     4261 cgtggcaaac gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct
     4321 gacaattgta cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat
     4381 gctactagtc gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct
     4441 tattttaagt gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg
     4501 ggaatatgct ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca
     4561 agatgatgta aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga
     4621 agaacagcat aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc
     4681 tgaaatgatc caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga
     4741 tgcttccata ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct
     4801 agcaacgaaa aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga
     4861 ggaattcgga ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga
     4921 aaaaaaatac ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa
     4981 aagcttctgg gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa
     5041 agcttttgat cagattgaca tcaaata
//
LOCUS       DAZUKD010000157         5066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000157 DAZUKD010000000
VERSION     DAZUKD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.161"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..1896)
                     /locus_tag="ACG8N9_002652"
     CDS             complement(130..1896)
                     /locus_tag="ACG8N9_002652"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898191.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1856..3610)
                     /locus_tag="ACG8N9_002653"
     CDS             complement(1856..3610)
                     /locus_tag="ACG8N9_002653"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898192.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3720..4289)
                     /locus_tag="ACG8N9_002654"
     CDS             complement(3720..4289)
                     /locus_tag="ACG8N9_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4898193.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4307..>5066)
                     /locus_tag="ACG8N9_002655"
     CDS             complement(4307..>5066)
                     /locus_tag="ACG8N9_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4898194.1"
                     /translation="KTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEI
                     TYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPF
                     LVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQR
                     LLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTC
                     NRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1683 a          951 c          685 g         1747 t
ORIGIN      
        1 gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa
       61 aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag
      121 cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt
      181 tttctttatg agatttttgt atatttctga ccttcgatac aactcattat gagtaccttg
      241 atcaactagt tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa
      301 gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat
      361 acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc
      421 atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact
      481 tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca
      541 gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg
      601 tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt
      661 gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc
      721 ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact
      781 agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat
      841 tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag
      901 aggactatct gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt
      961 aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat
     1021 ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag
     1081 taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa
     1141 ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat
     1201 ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg
     1261 caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt aatatttagt
     1321 attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg
     1381 caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg
     1441 gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc
     1501 ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga
     1561 taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac tttctttata
     1621 agtactatta tataagtttt catactccct atctaacaac caatacatga aaagcaaagt
     1681 aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa ctaattcgtc
     1741 tatcagcatc attataatga atgctggtac catattaatg cagtatacaa aaaaactaga
     1801 aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt tgtttttata
     1861 aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt
     1921 tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact attttccctt
     1981 ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa
     2041 taactgtttt ttcttttatc agatgttgaa aagccttttg tagttcatac tcattatctg
     2101 gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc
     2161 tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta
     2221 aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg
     2281 cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac
     2341 tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt
     2401 ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga
     2461 aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat
     2521 aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg
     2581 aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt
     2641 ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg
     2701 cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac
     2761 ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta
     2821 taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa
     2881 gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa
     2941 ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa
     3001 ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt
     3061 gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg
     3121 cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact gataaaacag
     3181 gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag
     3241 ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa
     3301 aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc
     3361 caaggatatg agaaaataag ccagaaaaaa tataaaataa agctcctaaa gttaaccaag
     3421 caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact
     3481 gaagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa
     3541 ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt
     3601 atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta
     3661 atgctttccg taatagcgta aattagagat gtacaaacag ataaaaggta aactacgttc
     3721 tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt
     3781 gctacaacta acactgaaat gattccccat atacctaccg catagtggat cattgcatcc
     3841 attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac
     3901 catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact
     3961 tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata
     4021 ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat
     4081 aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca
     4141 aatatgattc cagataataa tccaaaaatc ccgggggata tgctgtgacc aaacgcccca
     4201 ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac tgtagctaaa
     4261 ccaataaaaa tataatcttt tgttttcata tatattctcc tcaattctat ttcgttatgt
     4321 cattaaaaat ctgttctatc aaagttgaat cctctactct aagttttata tcttttttta
     4381 ttccaccatt ttttaaataa ataattctat tgcatgtttt ataaagaaat tccggatcat
     4441 gagtagctat aactactgca cccttttctt ttaagtcttt aataaggtca gcaatccttt
     4501 ccatattaat ataatccaac ccacttgtag gttcatcaaa tagaaataaa ttagtttttg
     4561 acaatgaagc tatagaaacc aataatcttt gtttttctcc accggataaa tcaaacggta
     4621 attgatcttt cattttataa agttccattt tttttaaaat tttttcttgc tgatctttac
     4681 taactatttc attccccagg gctaactcgc tcctcactga ttctgtgaaa aattggtaat
     4741 ccatttcttg cattactaaa aacggcgatg acaaattttt tttattttcg attatctttc
     4801 ctttggtagg gcgaataatc ttcatgagta ttcttagtaa ggttgttttc cccacaccgt
     4861 ttttccctac caaaccaata acatctccaa ctttgacatc aaaatttatg tcttttagaa
     4921 tattttcata agtaatttct tcaactttta atacatctgc acttaccttt tcgatatagg
     4981 gaacttcaat cgaactatta aaaatgtcaa atttccttaa tgcgttattg ttttcagttt
     5041 ttaacaactg aatagttgta gttttt
//
LOCUS       DAZUKD010000158         5044 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000158 DAZUKD010000000
VERSION     DAZUKD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.162"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1164
                     /locus_tag="ACG8N9_002656"
     CDS             94..1164
                     /locus_tag="ACG8N9_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4898195.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            complement(1130..1216)
                     /locus_tag="ACG8N9_002657"
                     /pseudo
     CDS             complement(1130..1216)
                     /locus_tag="ACG8N9_002657"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1260..1721)
                     /locus_tag="ACG8N9_002658"
     CDS             complement(1260..1721)
                     /locus_tag="ACG8N9_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS4898196.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(1981..2529)
                     /locus_tag="ACG8N9_002659"
     CDS             complement(1981..2529)
                     /locus_tag="ACG8N9_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898197.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2530..3387)
                     /locus_tag="ACG8N9_002660"
     CDS             complement(2530..3387)
                     /locus_tag="ACG8N9_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4898198.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3673..3960)
                     /locus_tag="ACG8N9_002661"
     CDS             complement(3673..3960)
                     /locus_tag="ACG8N9_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4898199.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3950..4279)
                     /locus_tag="ACG8N9_002662"
     CDS             complement(3950..4279)
                     /locus_tag="ACG8N9_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4898200.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4356..4628)
                     /locus_tag="ACG8N9_002663"
     CDS             complement(4356..4628)
                     /locus_tag="ACG8N9_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4898201.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(4628..4891)
                     /locus_tag="ACG8N9_002664"
     CDS             complement(4628..4891)
                     /locus_tag="ACG8N9_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4898202.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1516 a          981 c          722 g         1825 t
ORIGIN      
        1 ctttattaaa ttaagaggac agcatatcga tattgcttcc ttttttttgc tatttattta
       61 agggatagaa gtttagaaag gactgtttat aagatgaaaa atgatatagt gacttatgca
      121 aacacaaaat gtgaagaaat tggcatcagt aatttagaag agtgcttttt tgacacaaat
      181 gacctgtcgc aatttttaag aacgggaaga acaaatgcca gtaaacttgc caatgactta
      241 gttgcaaaga acgttctatt taaaatcaat actcgtccag tgttatttgc catcaaagag
      301 cgctttgaag agcacttttt gattccggta aaagatgtat acgaaagtat atctgaatta
      361 aaaacagact tggagcccaa ttctacagat tccattctac aaaagatgat tgggtatgat
      421 atgagcttat ccgatgcggt ggaacaaatg aaaactgctg tcttctatcc tggaagaggg
      481 cttcctatga tgatcactgg agatactgga gttggaaaaa gtttttttgt tcatactgct
      541 tatcagttta tgaaaaaagt gaacgttatt catgagaatg ctccttttaa agtacttaat
      601 tgtgcgcaat accataataa tcctgaatta ttgtcgggga tactatttgg ctatgttaaa
      661 ggtgctttta ccggcgcaga tgaagatcga aatggattgt tgcaagatgc agacggtgga
      721 gtactgtttt tagacgaagt acacagattg cctcctgaag ggcaagagaa actatttgtt
      781 tatatggatg gcggaactta ttctagagtc ggtgaaacta tccatcacgc ctccaatgta
      841 cgtttagttt ttgcgactac agaaggcaaa gaggattttc tggaaacctt tatgcgaaga
      901 atccctatac atatccatat cccaaatatt gaggaacgag gtattttaga aaaaaacaaa
      961 ttatcgaaaa tactctcttt agcgaaagca aaaactttag caaggctatt gatgtttctc
     1021 ctaaagtcac tagtgttgca taaatatagt cagaatattc tgaattatta ttttcttgaa
     1081 aaaagtaaaa aaaatcgtat cccaaacttt catctacatt tccagaaaga tcttcatgtt
     1141 gataatcaat tcctgaatca atgaccgcta ccttcacatc cttagaaccc ttggtttctt
     1201 cccaagcgga tggcatgcta atctctctta aattagcttg tttactccaa ttaggatcgt
     1261 tatcgatttc ctaaagaatc agttgtattt ggctcctcaa catcattcaa cggctctagt
     1321 tcaaccacat agtttggctc tgcataattg accatatcag aagcattaaa tgttcttact
     1381 gcctcttcca cactaatttc ttcgttcaat ttcactaata atacttgatc tcgcttatta
     1441 taatcgcttt catttgtcct aagagtcaaa tctttttcca ccataacttt tacacccaat
     1501 aaattttgcg gtttattttt ctctgttgaa attctttgtg tagcctttaa actgacaatt
     1561 agctcattgg gtacatactc tatttgagag tcatctaatt ggctcataat cctttctttt
     1621 tggttagtat ctgcagttac ttgtttagta tgagccatga acccaaacat actcacccca
     1681 acaaccaatc ctaaatatat tttcttttta aactttttca tctacatctc ctcctaatag
     1741 ttttatttta gagttagctc tattacttat ttactaactc aacaacagat tatcatacac
     1801 ttattaatga aatcacaata tctcattaaa acacacccta aaatcataac cgttcctatg
     1861 ctcatttttt ggatttttta atcacgtaac cagaaaaata aatcccctct atttcacgca
     1921 aatcaaagaa gaactaagta actaccgttt aaaggtagtt acttagttct tctttgatta
     1981 ttatctaact ttattattca ttccccaccc tttgcgcagt gagtcttcgg tatcttgttt
     2041 tatctgactg gtagttctta ttatttctcc acgatcatct aattctggaa caattggata
     2101 tttttctctg aacaagtcta gttctttcac cacgtcatta aacaaatctt gtttatcttc
     2161 ttgcgtaaga ttctgtttaa tatactcatc taaagctttt ctaagcaatt catctcttgt
     2221 catattagtc tcatactttt ctactaaaac tctggaagtc gcatttaaca aagtcgacag
     2281 ttttaaagga tgcttggtgg aataaacctt atcaaacttt ctgattggtg cgccaacttt
     2341 ttttgttctt cctctactct gtctttcaac attggaggaa gtttttattt cttgaacttg
     2401 gtgcacttct aattgcttat tttcagatgg tgtaattgct tgctgctttt tctttgcttc
     2461 tgcttctttt tttattttgg cgagtccttt gttgttccca aaaatatcaa gtcctttgat
     2521 gtcagccaat tactccacct ccgcatacca attctcgtgt tcttccaatt ctttcaaaat
     2581 actgataaat aattcgtggc ttttgcgatc ccattgtttt aagagacctt tctttgtctt
     2641 ctccatcgta atacctgatc cgtcgtatcg ttttaagcgc gcatttttga gtacaacatt
     2701 tttaatcaaa tgttccccat aaatctcttg ggcttgttta tacatttcct gatctactgc
     2761 gtcaccgctt tccaacatga aaggaataac accagcaaca tataaatttg tatcaaattc
     2821 tttcacaaaa aagtccatat actcaagata ttgcatcgca tttctcatcg attttacttg
     2881 ggtctgtaag acgacgattg cccaatccgc aatgtacatc gcggatttac tatatacaga
     2941 aatagaaggt gggacatcta aatagatgac gtcaaactca tcaaataatg gttctagctt
     3001 ttctttcata tatttgaatt gggataatga atcattatat tggtctttga tccaatcatc
     3061 atacatttca aaggacatgt ctgctggaag tatatagagg ttattgatta ctttatagac
     3121 agcctcttta ggtggaagat tgtttatcaa cacgtcagtc acagtatttt cgtaaacaat
     3181 cggttcacct tctccgcctc cttcttcgtt agtgaagttc atatgcgtta agttcatcac
     3241 atcagaagta tttccttgca tatccaagtc aattatcaag gttctttttc cttcattaac
     3301 tgctgattca aaccctaaca tttgcgcgca cgtactttta cctacgccac ctttaaaatt
     3361 accgattaca tatttcttcg ctaccacgtt cctacctctt ttcttttagc aatattcatt
     3421 tgatactatt ataacatatt tatttttagt tactaccaaa aaaaagatat atatgtacta
     3481 ccttataatt aataagtaaa aggtagtaca taaataagga aaagctgaaa tatattgatt
     3541 tatagctttc ctcaaagtaa tgacgttatt taatttatcg agaagcagag aatactcgcg
     3601 actttagtca tgtagacgaa tcagattttc gataaaaaag aagccgtcct tcttaagaac
     3661 gacaacaaat cactagatta attttttcaa ctgttcataa aagttttcat gcgttccaca
     3721 taacaatact ggaataacct tgttttctat aatctcgtaa gctactctat atgttgtgcc
     3781 cgcatatttg aacccactag accaaatacc tgataaatcc ccagtttttt gttcaccctt
     3841 atatggatca atcgcaattt catcataaat taaattcaaa aatttttctt tcaatggctt
     3901 ctcttttagc ttttttaaat acttaatgac atgttttgaa ggaacgattt cataagccaa
     3961 tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta tcttcagcaa
     4021 tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca agaatctcat
     4081 ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa tcataaaatt
     4141 ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt gcttcaactt
     4201 cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa ttgccttgtt
     4261 ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt acttagtaaa
     4321 tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta atgcgtcctt
     4381 gctgaataaa tttcaaccac gtgttcatct tcatctacct tataaacaac ccggtgttgt
     4441 ctattcaatc ttctagaata cctaccttca tgtgtaggtt ttaacttctc aaatgattgg
     4501 gtagggagat agggatcttc ctttaaagtc tgaataacct tctcaaattg aggtttcaaa
     4561 ttagactgtt tgattttctt taaatcagct ttagccgagt ttttaatcat cacagaatag
     4621 ttattcatta taagttatcc caatcaattt catcaacatt agtaaatcca ctatcatctt
     4681 tttctcgctt acgaacaaca cccaaagtgc ctatttgctc aagtaatagc gtttctttga
     4741 ttgctttcca atcatccaaa ccaataagca ccgcattatt ttcactgcgc tcactaatga
     4801 tttcaatttc cgtatgattc tcatttacat ctttcaacaa cttataaaaa tttttccgtg
     4861 ctgagctagg atttaagact gtttttgcca tatctactcc ccctctaatt tatatctcca
     4921 ttctaacgta cttaattaag tacgtaaatt cgattgtttt gtttaaattg gagtaccaaa
     4981 acagcattgt aaagtgttat ccgtttcctt tatacccgtg gtgctagtta attttttcct
     5041 agag
//
LOCUS       DAZUKD010000159         4845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000159 DAZUKD010000000
VERSION     DAZUKD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.163"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..322
                     /locus_tag="ACG8N9_002665"
     CDS             23..322
                     /locus_tag="ACG8N9_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898203.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            587..1462
                     /locus_tag="ACG8N9_002666"
     CDS             587..1462
                     /locus_tag="ACG8N9_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898204.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1459..1677
                     /locus_tag="ACG8N9_002667"
     CDS             1459..1677
                     /locus_tag="ACG8N9_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898205.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1664..4291
                     /locus_tag="ACG8N9_002668"
     CDS             1664..4291
                     /locus_tag="ACG8N9_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4898206.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4306..4524
                     /locus_tag="ACG8N9_002669"
     CDS             4306..4524
                     /locus_tag="ACG8N9_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898207.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1891 a          606 c          973 g         1375 t
ORIGIN      
        1 taattaaaaa ggagaggttt ttgtggaagc aataaaatta atgatttcag aagaacagga
       61 ggaaatgttt ctaaagcagt tttatcaaat tgctacaaaa gctattgaaa aggctcaaga
      121 aaatgcgggg atgacaaggg aatatctcaa tcaaaaagaa atggcggaat acttaaatgt
      181 gagtgaaaac tttttaaaaa atgctgtgag aaatgaggga ttgcccgtag ttgaattagg
      241 ggcaagaaaa ttttattcga aaaaggcagt taatgagttt atgtttgctc gaatgaaaag
      301 aggggaaaat attggaagat gatgaagtag aaattataaa aaaatgcttt aaaaaaagaa
      361 aaaaagtgtt cccaaaagtt tgcagactgg gggaacactc aataataaag tgctgagaaa
      421 actttattta tagctattat atcaaaaaag aaggtgaaaa ggtgttttct tctggaaatg
      481 ttgtagttga caaacttaga gaaataaatt ttgacggaaa tgtaatccac acacatggta
      541 cgatcaatta agaaaaaaaa cgaaaaaagg tgttgagaag ccctatttga atgcaatagt
      601 tatcttagca gaaattacct attggtatag acctaaaaaa atttttaacg atgatggtca
      661 gctacttggt tatcaaaaaa agtttcttga agatattttg caaaaatcat ataagcaatt
      721 aagcaaaaaa acaggattat cagaacgagc tataaaagat gcaattgtgt acttagagaa
      781 aaaggggatt atcaaaaggg tttttagaaa tatttattat gagaaaagag cagttacatt
      841 atcaaatgtt atgtacattg ttttaaatgt acataaattg atagaaatta cttttcctac
      901 tttggattgc gaaaaaaagg agtatatgcc gaaaattcgt cagaggggta tcgaaaaaaa
      961 gagatatgtc tctttaaaaa aaggtagagg tatgtacaaa tttgtatata cgtatacaaa
     1021 gactacttca cagaacatat caaagactac tctcgttatg gaagaagagg atattaatat
     1081 aaagaggatt tacagtaaga ttagtgttct ttatgacgca aataacgaag atggtcgaaa
     1141 attatttcaa gtgtatcact atttgagaaa cgctaatgta gatacagaag atatcagaaa
     1201 aatcattgaa tatttatctg aaaatactca gtatcttatt gctgagtgta ttataaaaca
     1261 acatgaagca tgcttttttc aaagaaaaac tgaagaaggg ttatacgatt atgtaacata
     1321 ttttttgaat ggacttcaga aaagatataa aagtcaattt ttagaaaatg tagagcctat
     1381 agatcagttc aaaaaagctt tcgaaatcaa agagttacct gaaattacta tgcataattg
     1441 gttgaaagga gaagaatcat gattaaaaat cgcttaatag atcaaatgtg gtgtcttgat
     1501 caaaatacag atactctcga attaatcgag agtatctgct tcaacacagt agttctcgat
     1561 ttaagagctg aaaatgacaa ttgtgtaact catatcataa ttaatcaaaa aatggctcag
     1621 cagctctctg agagcctaag aaaatgggct gaaggagaaa actatgaaag caattaaaga
     1681 aaaaatcgtt agtagaaaaa taaactggtt agcactcagt gtacttgttt taatgattag
     1741 cgttgttttt acaagtaata ttgttgaagc tagcattttc aattggtttg atgacacaaa
     1801 aagtcaaaca gagtttttga ataattctct ttatagacct tacctaaaaa aatacaatgg
     1861 agatttcctt agtcagttcg ggatttggct tggatgggct gtggtaaaag gcatgtttac
     1921 tgtgactgat tcgattcaaa atatgattcc agatgtgttg gatttgttca atttcattga
     1981 aagtacaggg ctaaataatg tttatcaatc agtcataaat acaattgtcg ttggattaat
     2041 gatcctttca ttgatgtttg ttggctacaa gatgattact ggaaaaggaa caatagattt
     2101 aagatctgtt ggaatgaata tagtgatgtc tgtagcgtta attcttttaa tgccaacaat
     2161 gatttctagc ggtattcaat ttgccaaaat attttacaat gatgctacta cgattacaaa
     2221 ttcagatgac ggagtcgctt ggtcattgat aaaacaagga gtaacagatc tggcatatat
     2281 taataaaaca gatcaatact caagcattga taaaacggag gatcgaaata agttaacacg
     2341 aaaaaatttt caacagacag atttaacaca agtgttgacg gataaagtga ttgataaatt
     2401 ggaaaaagaa aatccagctg cggataatct tcgctatgaa ttagttgaaa attctaataa
     2461 tgaatttgtt gccacaaagt tttctgataa cttcttatca acattttctg atagcttgaa
     2521 atcaggttat tatcgatatc aagcaaatct ttgggggatt tcgattgggt tgacagcgtt
     2581 ggcaatcgcc tatgtgttca gcgcgtttgt gatcattaca gcaatattag aacttgcatt
     2641 taaacgtgtg ttaggtgttc ttgtttttgc gacagatatt gaaacaggac aacgttcaaa
     2701 agttgtttta tccgatattt tacaatgtta cttaacagtt ggatttcaag ggtttggact
     2761 atctatgttt gctatgttta tcaatttttt aaatgctgga caaggtatct ccacaaatat
     2821 ttttataaaa acaattgctt atatctgtgc tgtgtttgtg ctgataaaag gcagtggaac
     2881 agtcatgaga tactttggtg ttgatattgg actcaaagaa ggctatgggc aacttgcttc
     2941 tgcttttggt atgggcgcaa tgctgtttag aaaaggatca actggattta atcgtgctaa
     3001 aggaagcgga aatggaaatg gatcagacag tggagaagga gaagatcgaa aaccagaaaa
     3061 gaatttcggc gaaactctat caaaaaaagc tggcaaaact ggaagagctt tgggatatgc
     3121 ccatgaacgt ggattatcag gtttagcttc agatggagct acaatggcta gtgaaagagc
     3181 aataaaacca tttaagagta tgcgtgatat ggctaatgat accaagaata agttcaaaga
     3241 aggtttagat gatggtacag tgtccgctat taataaaaat agcaaaccta tgttagctaa
     3301 aaataaagaa gatgaaactg gaaaatatgc tgattccatg ccaacacgtc tttcggatag
     3361 aaaagatggt gctaaggttg aaaatgccga tagaattatg tctagttctg aacgtatgcg
     3421 tgaagcaatg aaaaataatg ctgaatcgaa taataatcct gtaagtgcaa ttcagcaaaa
     3481 agttcaacaa gatattgaag aacgtaaaaa tgctatgcac ggtcaagcta agtctgctga
     3541 agaattaatt aatcagaagc gacaagaagc taaatacacg cctgaagcaa tgaatcgtga
     3601 ggaaatgtta aggaaacgtg tagaagggcg tacaggagct aatacggacg aaaaagaagc
     3661 gttgaccaaa gaaagaatcc aagaagctaa aaactcaaat accggattag aaaaattggt
     3721 caaagaaaat ctacaaaatt ctacaagtag tcaaggtggc cgatcagtag atgtccgaga
     3781 aaatattcaa ggttcattga atggtcatgt cggacggaat gttgatgtaa gagagaattt
     3841 acaaagctct tcaaatggtc aaggtactaa gacaattgat gttcgagaaa atgtacaaaa
     3901 ttctacaagt agtcaagggt ttaaatcaat ggatgtaaga gagaatgtac aaaattctac
     3961 aagtagtcaa gggtttaaat caatggatgt aagagagaat gtacaaagtt ctacaagtag
     4021 tcaaggattc aaaacagtag atgttcggga gaacgttcaa aaagatagtg gagaaatgaa
     4081 agaaaaaaca caaaaaatca atatcgttga agacagaaag tcagcgaaaa aatttgattc
     4141 aaatcatgaa acagtgatta ttgatagtga aatccgtgaa aatgataagg gatcaaaacc
     4201 aagacgaaga tttacctatg aagacaacga gttattcaga gatacactta atgatcctaa
     4261 tccgcttttc gataatctat taaaaaaata aaagggggaa ttgtcatgaa attaattcta
     4321 aagattagta cgcatgattt cttagaactt gacgaattat gtgaatcagt aaagaacgca
     4381 acaaatgaaa aaatcacggt cactaattat attgaactga tgattcaaga agaattaaag
     4441 atgaaacatg agataaaaaa caacttgaaa tattgttatc tagaaaaaag tgaagagata
     4501 atccatgaat taaatggctt ttaaaaattg tatttgacat gaatttacga caaggaatta
     4561 gtggggatta agcgattatt ctcttccact ttagagaaat aatgggttaa aaagccagaa
     4621 tacgtagagc aatattgaca acctataaag gagctattgt tgaaaattga agtaacaaaa
     4681 agataaagag aggtttacat ctatcagatt aaaagtgata gtacacccat gaaacctgca
     4741 aaagagttga acctaatgta tatcggttta actcttttta tttgcaaatt aaaaaaatat
     4801 caccgaagtg acgaatgaaa ctatgaccaa attaagcaag aaaat
//
LOCUS       DAZUKD010000160         4825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000160 DAZUKD010000000
VERSION     DAZUKD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.165"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG8N9_002670"
     CDS             complement(207..1169)
                     /locus_tag="ACG8N9_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4898208.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG8N9_002671"
     CDS             complement(1171..2610)
                     /locus_tag="ACG8N9_002671"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4898209.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACG8N9_002672"
     CDS             2795..4741
                     /locus_tag="ACG8N9_002672"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4898210.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1542 a          823 c          792 g         1668 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttat
//
LOCUS       DAZUKD010000161         4818 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000161 DAZUKD010000000
VERSION     DAZUKD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.166"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="ACG8N9_002673"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="ACG8N9_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4898211.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1525..1720
                     /locus_tag="ACG8N9_002674"
                     /pseudo
     CDS             1525..1720
                     /locus_tag="ACG8N9_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3252..3770)
                     /locus_tag="ACG8N9_002675"
     CDS             complement(3252..3770)
                     /locus_tag="ACG8N9_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4898212.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4137..4385
                     /locus_tag="ACG8N9_002676"
     CDS             4137..4385
                     /locus_tag="ACG8N9_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898213.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1581 a          787 c          734 g         1716 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagggact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccacttag cttggata
//
LOCUS       DAZUKD010000162         4794 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000162 DAZUKD010000000
VERSION     DAZUKD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.167"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACG8N9_002677"
     CDS             <1..525
                     /locus_tag="ACG8N9_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4898214.1"
                     /translation="KQYRTKGIAYFSKEDFHELLDIPKSYRESVINKKIIKPIRQELT
                     AIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQI
                     YSEKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPEL
                     KKGLLDNLQNLFKE"
     gene            535..1032
                     /locus_tag="ACG8N9_002678"
     CDS             535..1032
                     /locus_tag="ACG8N9_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4898215.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1408..1689
                     /locus_tag="ACG8N9_002679"
     CDS             1408..1689
                     /locus_tag="ACG8N9_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898216.1"
                     /translation="MDILVRKIDSKIISEIDRRCQELTTKTSKKWSRNTYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNEYVRTNNQLIETLIGGL"
     gene            1691..2107
                     /locus_tag="ACG8N9_002680"
     CDS             1691..2107
                     /locus_tag="ACG8N9_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898217.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISILSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNHQKETNQTFTAGNFDPTNFLKR"
     gene            2765..3190
                     /gene="bacA"
                     /locus_tag="ACG8N9_002681"
     CDS             2765..3190
                     /gene="bacA"
                     /locus_tag="ACG8N9_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS4898218.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            3220..3384
                     /locus_tag="ACG8N9_002682"
     CDS             3220..3384
                     /locus_tag="ACG8N9_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898219.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            4344..>4794
                     /locus_tag="ACG8N9_002683"
     CDS             4344..>4794
                     /locus_tag="ACG8N9_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4898220.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRL"
BASE COUNT         1744 a          816 c          804 g         1430 t
ORIGIN      
        1 aaacaataca gaaccaaagg aattgcctat ttttcaaaag aagactttca tgaattactt
       61 gatatcccta aaagttatag agaaagtgtt ataaataaaa aaattataaa gcctattcgc
      121 caagaactaa ccgcaatctt caaaggtctg acaatcaaaa agaaatacgg aaaaggaaga
      181 ggaaaacctg tcattggcta tcaattcacg tttaaacctg aaataaaaaa agcagacgac
      241 tttaataaac acgcaccaac aaaagaagaa caaaataaag ctttagaact agttaatcaa
      301 caaatttact ctgaaaaaga ccccacttca ttacaagaaa aaatcaaaga acagaaacga
      361 aaattagaac ttttacaaca attagaagag ctagaaagaa aacaaaataa tctgaataaa
      421 gaaaataagg agattaacga acaaatacca gataacaatt ccgatattcc cccagaatta
      481 aaaaaaggct tactagataa tcttcaaaac ttatttaaag aataaggggt ttttatgaca
      541 gataaaacta tcaaagaatt agcagatatt ctaggtgttt caaaagataa agtgaagtat
      601 caactgagga aattacctag taattttacc tacaaaaaag gaaatattat ctacctgaaa
      661 aaagaagcag taacaacaat tacaaaggtt ttatcaggta atgagccggg taacttacct
      721 agtgaattac ctgattcatt acctaccgat tacctactca aggagattga aactaagaat
      781 aatcatattg aagaaaaaga taggcaaatt gaaaaacttc aactagcttt agaaacacaa
      841 caaaaattac tcgaccagca acaacaactc accctgcaag ccaaccaaca aatcgagaaa
      901 ctgcaggaac aactacagtt gacttatacg gaagaatctt cggaaaataa atcaacgact
      961 ctttctgaag aaacagaatc gatagaaaaa caatcaaaga cggagaaaaa atggtggcaa
     1021 ttctggcaat aaaaccagca atttaaatta ataaaaattg ctggttttat tcttcttttt
     1081 gcttttgcga gcagagcaac atgggcccat gttgcgatat tttggtggcg ccccaatccc
     1141 cgttttttcg ttgaatctta ctcggactgc actcgtttta cagctgattt ttctgaattt
     1201 tgaaagagca taaggaaacg tggtatcact ttcttacgaa aggtgctaga taagctgtgc
     1261 ctatcacgtt tctcagaaaa aaatttgcta tcactttttt gccattatgg atagtgtatg
     1321 tatagtattt tggtaaaaaa cagctataca aatactatat ttgtatgata tacatagatt
     1381 atacgcacat gcgaaaggaa aattgctatg gatattttag ttcggaagat tgattcaaaa
     1441 attatttcag aaatagatag acgttgccaa gaacttacaa ccaaaacgag caaaaaatgg
     1501 agccgtaata cgtacctaaa agttctaatc gaaaatgatt ttgaccaggc acttaggcat
     1561 tacaaaaaag atcaattcga tttattactc gaaaaattta tcgatataca agcaaaaaat
     1621 acggaaacac tgaatgaata tgttcgtaca aacaaccaac tcattgaaac acttatcgga
     1681 ggactataac atgagaaaaa cttctgttca aaaacacgtt accctttatc ccagtcacgt
     1741 ggacgtttta gattccatta ttgaaagaaa tccagatatc aacaattact cggaagccat
     1801 tcgtttttta tgtatgagtt atgaacctga taccaacaca accactacta gcaaccaagc
     1861 caaactaaat gctatgggaa aagaaatttc tatcctgtcc gaaatcattg cagaatttgc
     1921 tgatttatat ctaaaagata ctggtgttct tattggtacc aattctaagg tgtatcaaga
     1981 agtgaaagaa aaaattgaaa atcaactcaa tccttcccga cgaaatcaac ccaaaaatca
     2041 tcaaaaagaa accaatcaaa cattcactgc tggaaatttt gacccaacca actttctaaa
     2101 aagataaaaa cagaacctga aaaataaaaa aattcctcga tttcttttta gggggggcgg
     2161 tcttccagca agatttgcaa gttgggacca acttgcggaa aaaaagaaaa attcgcttgc
     2221 gcgaatcctt ctttttgcat tttggggcgc tgccccaatc cccaagtatc ggttatttaa
     2281 tagcgtggca actcaaagaa cagaacatac tctgaaacaa aaaaagactg ctctaacgag
     2341 cagccagtta aaaaataatg agtgtttatt cttgaaaaag caacgcgctt atgaagcgct
     2401 accaaaaaat tatcatattt tattaaaaat aacaacccag taaaaaatga ctggtaaaaa
     2461 ttcacaaaga aacggaaaga agcaaccgca gaaaatcaag cttaaaaaga gcttttgacc
     2521 tagaccaatg aaaaatcaca aagtccgccc ctgtacctgt tggtagctgg gctacttggc
     2581 atactgtaca ttatgtcaac aagaatttaa tataacatat ttttctagtt tgttataaca
     2641 atgccactct aacaaaaatg taagcattat ccttattaaa agctccttga caagactata
     2701 ccttttattt tatgattttt acatagaaaa cgttttctct attttttgtt tagaggtgat
     2761 ttttatgatt tgcaagaaca agaaaaaaat tattgctatt attgctagtt tctctttgat
     2821 ttttagtact gcatgtactg tcttattaac aaataatgct aatgttgaag ctgcaagctc
     2881 ttcttacaaa cataatcatg ctggttatag atgttacaaa atgagttatt atgtaagtcc
     2941 ttctaaatgc aaacaattgg ctcgccaaac taataaactt aataccgtct cagctattgc
     3001 aaactttatt ggactatctg gattaacacc ggggattata tcattagttt tcggatcagc
     3061 agttagtgct aataatgtat ttgttacagc cgctagaaga ggaaaaggcg ttcaattaaa
     3121 ttatattgcc cattttagta ataccactac ctataattat agttctaacc aaaattatgt
     3181 tataaaataa ttaaaacttt aaactaggag gaatgcttta tgaaattaac aaaaattgct
     3241 gtaattttag ctgttattgc tggcattatt ttatattttg tattgcctgc taatcaattc
     3301 ttcctttact ttggaatacc tgcgatttta atagtaaatg gcgtaagaat ctttgaacaa
     3361 cgaaaaaaat gtagctctac ctaatcgtta caaaacaaca atttcacctt cggtgaaata
     3421 agcgaaactt tacaaaaagt ggctagcaaa tgctagcctt ttttgtattg tagagcgtga
     3481 attttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     3541 atacctccgt atttggctag caatccttct aaaaaataca cctatcgatc agagtcaaaa
     3601 caaatcaatt ttcgagtgag cgaatccgaa tttgcaaagc tgaagctttg caaatgagtg
     3661 tgccggcttc cgtaaaaata catatggcta taatttgcgt tttaaggcgt tttaaacgtt
     3721 ctggtatata aaattatacc aaaacaactt taaaacgcct tagatctaat tttagaggtc
     3781 ttatagaaca ttttagggtt gttagggctt gccctgaccg cctgtaaggc gctttgagaa
     3841 caagataagg gttttatact aagactgtct taaaacggct taaacaaacg ctcagaactt
     3901 ttcaaaggag ttctttatga actgttttta agcaaaggag cctttaggcg actgcgcagt
     3961 ataatgtgag agcgtagcga tcacacattt ttagtgaccg tagggagcga gtttcccagt
     4021 ttagaaaaat tcgcgaactt gtttgaggaa aaaagcagga gttttttagg cgggaaattt
     4081 tgcctcctct tttataatct ctttttaaac ctctttttaa acctctttac cgtcggctgt
     4141 atgcttactc tcccaaggtg tgcagaatct tttgggggtc tttttgtctg tttttagggg
     4201 tctttttgtc tgtttttagg ggtctttttg tctgtttttg ggggtctttt tgtctgtttt
     4261 taggggtctt taattagggg cttgacccct attagaaagc tctttataat tttgttaagt
     4321 agttaatctt gaaggaggat tttatggcaa atgaaattgt gaaataccac catgaattaa
     4381 atacgattcc tttacgcaaa tttacgtcag tagaaatgaa tttattcttc tctatagtat
     4441 ctagaatgcg tgacgtaggc gataaaaaag tacaatttac attcgaacaa ttaaaagatt
     4501 taagcaatta taaagcaact gcaaatgttc gattcataga tgatttagaa acaacgtatg
     4561 acaaattaat ggatttacgt tttggtagaa gaagtgctga cggtttgcaa cgtgaacgat
     4621 ttgttttatt taatcaattt aaaatcgatg gaaaagctga tattcctttt gctgaaatac
     4681 aagtccatga gaaagcctta cccttactta ataatcttga agaatgggtt cgatattctc
     4741 tccaacaatt caatgaatta gaaagcagtt attccaaaac catgtttaga ttat
//
LOCUS       DAZUKD010000163         4777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000163 DAZUKD010000000
VERSION     DAZUKD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.168"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..1745)
                     /locus_tag="ACG8N9_002684"
     CDS             complement(135..1745)
                     /locus_tag="ACG8N9_002684"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4898221.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1967..2212)
                     /locus_tag="ACG8N9_002685"
     CDS             complement(1967..2212)
                     /locus_tag="ACG8N9_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898222.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2938..4278)
                     /gene="glnA"
                     /locus_tag="ACG8N9_002686"
     CDS             complement(2938..4278)
                     /gene="glnA"
                     /locus_tag="ACG8N9_002686"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4898223.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4306..4698)
                     /locus_tag="ACG8N9_002687"
     CDS             complement(4306..4698)
                     /locus_tag="ACG8N9_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4898224.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1400 a         1038 c          788 g         1551 t
ORIGIN      
        1 ctttaatcga aaaaagtctt ttgctttcta tcttttttcc tacaaataat aacaaatcag
       61 aaaaagagaa aagtgacaag aaaattgctt atccccttct cttgctactt ttctctttct
      121 atcaagttca aaaattattt tgtttcgtga tctgctaatg atgctccgac aaatgctttg
      181 attaattttt gcgggcgatt tggacgagaa atcaattctg ggtggaattg gcatcccaca
      241 aagaattttt tctctggcag ctctacgatt tctactaaac ggttatctgg tgagacacct
      301 gaaaagacta gcccttgttc ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg
      361 tgacggtgac gttcttgcac aacatcggct ccatcatatg cttcagccgt cttcgttcct
      421 tttttgattt tacatgggta taatccaagg cgtaaagttc cacccaaatt ctcgatgttt
      481 tcctgatcag ccattaaatc aataatattg tttgctgtat ctggattcgt ttcagctgat
      541 gacgcatctt ctaatccaac gacgttacgt gcaaattcga cacaagccat ctgcatacca
      601 agacaaatac ctaaaaacgg aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc
      661 ccttctactc cgcgatcacc aaatccacca ggaaccaaga ttccatctgc gtctttcaaa
      721 cgttcttcta cattttctct cgttaattct tgtgctttga tccaatcgat ctcgatgtct
      781 gaattatagg cgaaaccaga atgtttcaat gcttcaacta ctgagatata tgcatctggt
      841 aactcgacat atttaccgac aagcgcaatc cgtacttttt tcttcaagtt caagacacgt
      901 tcttctaatt ctttccattc tgtcatgtct gcttctggtg tatctagttt taaatggtcg
      961 caaacaattt gatccattcc ttgtgcttgt aaaactaatg ggatggagta aagtgtatct
     1021 acatctggcg actcgataac tgcttctggt tcgacatcac aaaattgtgc caatttgttt
     1081 ttcacgcttt gagaaacagg ttgctctgta cgtaccacca aaatattcgg ttggatacct
     1141 aaaccacgta gctcttttac actgtgctgc gttggtttag ttttcatttc tccagcagct
     1201 ttcaagtaag gaatcaatgt tgtatggata tacataacat tatctgtacc gacatctgct
     1261 ttcatttgac gaagtgcttc aaggaatggc aaagattcta tatcccctac tgttccccct
     1321 acttctgtaa tgatcacatc tgaatcagtc attttagctg ctcgcatgat tttttctttg
     1381 atctcatttg taatatgagg aataacttga acagttgctc ccaagtattc gcccttacgt
     1441 tctttacgca aaacttcaga atagattttt cctgtcgtca cgttggagta tttgttcaaa
     1501 ttgatgtcaa taaaacgttc gtagtggccc agatccaaat ctgtttcagc accatcatct
     1561 gttacgaata cttctccgtg ctgatatggg ctcatcgtcc ccggatccac gttgatgtat
     1621 ggatcaaatt tttggatagt tacttttaag ccgcggtttt tcaataagcg tcctaaagaa
     1681 gctgcaacaa tcccttttcc gattgatgaa acgacaccgc cggtcacaaa aatatatttt
     1741 gtcataagtt caaaaactcc tttttaattt atgtgcagga aaagtctcag acaataaaca
     1801 aagctcccta ttcttcaata agaataggga gccgaaattt ctgtaaactt ctgaccctct
     1861 aaaagggtgc ccaaggtgta taatacaagc tgactaaggg aaagtcaagt actcaaatga
     1921 atactatccg cttacaattt tttctttttc gatatccttt gagaaattat ttcttaagtc
     1981 ctttatgagt gcaaaaaaaa tttaacccac ataataagct tgatataaac atagctaacg
     2041 ttaaacctaa gcaaatcaga aaatcgttac tgaaaaattt taagtgaaat aatagataaa
     2101 aaaataaaac aaacattatg atagataata tcccacctaa caaagaacta ctttttccta
     2161 cttgcagcaa cagtttatat agcttatcgt agaaacaatc tttttctttc acccttattt
     2221 acctccttta cgtaaaaaac gtagtccaca gttctcggat ttttggcata tcttttctat
     2281 taaataatat caaaaacaaa acaattccac taattttttc aaagatgacc tttttttcaa
     2341 caaaagtgcc ttgtagggat tcattgaagg taacgaatca cataagtaaa aaataaggag
     2401 atgcgacaaa agtcgtgcct ccttatttct aaataaacgg tgttctcctg ccaactcctc
     2461 ggtcacaagt cacaatatcc atcaaacatg agaagctgtt tttgaaatta tttcttgtga
     2521 ttcggagtta aatggtaggc tcacaacctc tggataaacg gtattcaaaa gccagtttct
     2581 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa
     2641 tactcgaaac taaacgtctt tttcaaaacc tcttggtaaa cggtgttcaa aagcttgcat
     2701 ctgcggtaat aagttcagcc aattcaaaaa caagaaaagc tgtttttgct tggctattct
     2761 tattacttgt tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct
     2821 gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc cgttagtttc
     2881 tctaatactc aggtcaaaac gctgatttca cagcctctaa ttcacggtat tcctgtttta
     2941 ataaagttct agatattgtt ctctttccca ctcggaaaca gtttgtctga atgccgccca
     3001 ttcgagacgt tttgcttcaa caaaatttga gaagatatgt tgtcctaatg cgtcaatcat
     3061 tacattatcc ttacgcaatt ctttgatagc attatggata gttgaaggta agtcctggat
     3121 atgtgcagct tctctttctt cctcatccat tacataaatg ttacggtcaa cagctgctgg
     3181 cggtgtgatc tgattacgga taccgtctag tcctgcttgc aacaatactg ccattgccaa
     3241 gtatgggttt gcagatggat caactgaccg caattccaaa cgtgtggata aaccgcgaga
     3301 ttctggtaca cgtactaatg gcgaacgatt acggccactc catgcgacat aaacaggcgc
     3361 ttcataacct ggaacgagac gtttataaga attgactgtt ggattgcaaa cggctgtgta
     3421 ggcacgagca tgtttcagca aaccacctaa gaaatgataa gctgtttcac ttagttccaa
     3481 ttcaccgtct ttatcataga aaacatttcc ttcatcattg aacaaagaca tattgcaatg
     3541 cataccagaa ccattgatcc caaataacgg tttcggcatg aaagtcgcgt gaagtccatg
     3601 cttgcgtgca atcgttttga caacgagctt gaaggtttgg atattatcac aagcatctac
     3661 cacatctgca tatttaaagt caatttcatg ctgtcctggc gctacttcat gatgtgaagc
     3721 ttccacttca aagcctagac tttctaattc caacacgatg tcacggcggc aattttcacc
     3781 taaatctgtt ggtgcaaaat caaaataacc acctttatca ttcaaggtcg ttgtgatccc
     3841 accgtcttcg tctaatttaa ataagaagaa ttctggttca ggtcctaaat tgaaagaggt
     3901 aaaaccaagg tcgcgcatat ctttcaatgc tcgtttcaaa ttcccgcgag gatcgccggc
     3961 aaatggtgtc ccatccggat tatagatatc acaaattaaa cgagcaactt ttccatgagc
     4021 actttcccac gggaagatca tccaagtgga gagatctgga tacaaataca tatcgctttc
     4081 ttcaattcga acaaatcctt caatcgaaga accgtcaaac atcattttat tttctaatac
     4141 tttgtcgatc tggctcactg gaacttctac atttttgatc gtacctaaga tgtctgtgaa
     4201 catcaatcgt aaaaatcgta cgttttcttc ctcaatgatt cgtttgattt cttcacctgt
     4261 tatctgtttc tttaccatgg tatccctctt ccttttttaa tttattcagt caaagaattg
     4321 tctttgcaat ccttgcgact gataatgacc tgttttgctt aacccacctt gcgccaaaat
     4381 ctcatcacga aaaatttgtc gaacgtcatg atctgtcaac ggcttcttgt tttttgcttc
     4441 ttgttttttc gcctgtgcca tttcgtatac acgtttgatc ccagccatat tcaatccctc
     4501 tgaaagatag tctttaatct caagcaacat atcaatatca ttcagcgaat acatacgtcg
     4561 gtttccttcg cttcgttccg gatgaactaa atcttgttct tcataatagc gaatttgccg
     4621 agcagacaaa tcagtcagct tcataactgt accaattgga aatacggaca tagatcggcg
     4681 caattccttt tctcccatca gacgtcccct ttcgttttat aagttggcta aagtgtacca
     4741 attaaaaaaa atattgtcaa ctgcatatgt taggttt
//
LOCUS       DAZUKD010000164         4730 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000164 DAZUKD010000000
VERSION     DAZUKD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.169"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1054
                     /locus_tag="ACG8N9_002688"
     CDS             95..1054
                     /locus_tag="ACG8N9_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HFS4898225.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="ACG8N9_002689"
     CDS             complement(1487..2059)
                     /locus_tag="ACG8N9_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4898226.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACG8N9_002690"
     CDS             complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACG8N9_002690"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4898227.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2860..3597)
                     /locus_tag="ACG8N9_002691"
                     /pseudo
     CDS             complement(2860..3597)
                     /locus_tag="ACG8N9_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4040..>4730)
                     /locus_tag="ACG8N9_002692"
     CDS             complement(4040..>4730)
                     /locus_tag="ACG8N9_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFS4898228.1"
                     /translation="YQYSNLTLIAEIARYLNFSPLEVQRILTESQDGLEQILFWSNTY
                     KQVVYEILIPKIFEYKFKITKSQITEDNEVVLLKFPQGKNYYKFSGKPELVIMDSDKQ
                     LENYKTKSFHADTYVFDSKPERELFLQYLENDTVEEIFFTGMFTSKQGDFYIQYIDPE
                     ANSVRSYFPDFVVKKRDGSREIIEVKGDNKKDDPVVLAKKAATEEQAGQSGYSYRMIL
                     GSEIMNNNYEI"
BASE COUNT         1436 a          916 c          804 g         1574 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc ctctacaaaa ttcacaatag atataatcta ttgaaaattt
     2821 aaaagaagac aaaatagagt acagaaaatg ttttctgtac tctataaatg attctgatac
     2881 ttcattgaag tccatggctt taactaatcc atcacgacgt aataatccat ttgaatgttc
     2941 atttagagcg cgttgtgacg gggtgcctgg atccgcaaaa taaatatcaa tatcattttg
     3001 atttcccata gctttccaat ttgaaaattc tttcccacag tcaaacgtaa ttgatttaaa
     3061 cagatgttta ggaaccgttt gaaaccaatg attaattcga ttttcaatgt ccattgcctg
     3121 tctaccggct ggcttcaatg taataatcac ttttgacaaa cgttccacca atgtaataac
     3181 agcacttttg tgttttcgac cgacaatagt gtctccttca agatggccaa actcgttttc
     3241 aaattctgga tgctccttga tgcgctcatg tatactgcgc cgaaaagctt gtttcccacg
     3301 tttttcttgg tgtccatttg gttttcgttt gcctttcata ggcaaatcac gcacattaaa
     3361 tgcgccagtt tgaaacattc gatacagtgt acggactgaa caatcaagtt tcatttccga
     3421 gcggccgaca atcacatccg gtgtccaccc ttgaacaacc ctcttttgaa catattcttt
     3481 ttgttcctct ggtaaagcaa tcgagcgtct accgcagcgt tttttgtttt ctttgtattg
     3541 ttgatagtag ttcagtatcg tagcaccagt tttcaaaaat tgatacacgt tatgaatcgt
     3601 ttgtcacgca cgtcctaagc attgcgcagt tttggcaacg gattgattat ttttgaaata
     3661 agactctatg attaccagtt cgtctgttgt aagatgggtg taggtcattt gtactcactc
     3721 cttaataatt ctttggtcgg aactattttg agtgtagcac aaatgatctt tttagttgtc
     3781 tagcttaatt ttacaatcga cgaaaagaat actatgatga gattagtttg aaatttcatt
     3841 ttgatgagta gatattagga actgattgat agaacgaata agttatattg atattttgta
     3901 tttgcagtat ttgaaaaagt tgaagatgat aaggaaaata aagaaacaac aggaacagtc
     3961 taacttgact attcctgttg ttgaaaagat tatgattaat ttactacttt attttttata
     4021 gacatgctat ttacatactc tatatttcat agttgttatt cataatttca cttccaagta
     4081 tcatcctgta tgaataacca gattggcccg cttgttcttc tgttgctgct tttttagcta
     4141 ggacaacggg atcatctttt ttattatctc ctttaacctc gataatttcc cgacttccat
     4201 ctcttttctt gaccacaaaa tccggaaaat aactacgaac agagttggct tcgggatcga
     4261 tgtattggat gtagaagtct ccttgtttgc tggtaaacat tcctgtaaag aatatctctt
     4321 cgactgtatc attttctaga tattgtaaga aaagttcgcg ctcgggtttt gagtcaaaca
     4381 cataggtatc ggcgtggaag ctctttgttt tataattttc taattgctta tctgaatcca
     4441 ttataaccag ttctggtttg ccactaaatt tgtagtaatt ttttccttga gggaacttca
     4501 agagaacgac ctcgttgtct tctgtaattt gagatttagt tattttgaat ttatactcaa
     4561 atattttagg gattaatatc tcataaacta cttgtttata ggtatttgac cagaagagaa
     4621 tttgctctag tccatcttgc gattcagtta ggattctttg tacctctaga ggactaaagt
     4681 tcaagtatct ggcgatttcg gcgattaaag ttagattgga atattggtat
//
LOCUS       DAZUKD010000165         4657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000165 DAZUKD010000000
VERSION     DAZUKD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.170"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..1134)
                     /gene="murB"
                     /locus_tag="ACG8N9_002693"
     CDS             complement(199..1134)
                     /gene="murB"
                     /locus_tag="ACG8N9_002693"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4898229.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1207..2127)
                     /locus_tag="ACG8N9_002694"
     CDS             complement(1207..2127)
                     /locus_tag="ACG8N9_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4898230.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2240..2992
                     /locus_tag="ACG8N9_002695"
     CDS             2240..2992
                     /locus_tag="ACG8N9_002695"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4898231.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3856..4389
                     /locus_tag="ACG8N9_002696"
     CDS             3856..4389
                     /locus_tag="ACG8N9_002696"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4898232.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1377 a          904 c          821 g         1555 t
ORIGIN      
        1 tttcatcagt tattctcatt ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa
       61 tttatgagga aatattctat attttttgtt ttattgtgtt aaaatgtaca agatttgcgc
      121 catttgcgta aacaaccaca aaaaagagca ggacggccac cgtcctgctc ttttacttat
      181 tttaagttcc attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt
      241 ttctccaatg atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc
      301 ttggatgtgc gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat
      361 gaatccagca tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc
      421 ttgttggatc agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga
      481 agggtattct aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt
      541 gattgcttca taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa
      601 ttcttggact ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa
      661 tgttccatct tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata
      721 cgcacctgca ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc
      781 aaatccagtc aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc
      841 ttccgcaatg accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac
      901 tacaccgcga atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca
      961 aggtatatca tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg
     1021 gaatgcaagg acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc
     1081 atcaaacaat atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga
     1141 ttttcctttc ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag
     1201 tcttgttcaa agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact
     1261 tcttacaact tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga
     1321 aaagaaggcc tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc
     1381 aaattctttc actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac
     1441 ttcataagta ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa
     1501 atccatcgtt aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa
     1561 aaattccttt gtttctctta tctggtattt tgttctcaaa atgggttcat ctaataaata
     1621 cacagctgta tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt
     1681 tgccgctatt cgattttttt gcaggtggat caaccgctta tctgggatac gtttcaattc
     1741 ttctaccata ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc
     1801 agctaattct tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata
     1861 aacatggata ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc
     1921 gtggatcata catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg
     1981 ttggaacccg tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata
     2041 aaaatcaagt gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt
     2101 ccctacaccg attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca
     2161 tcttacacca ttttttacta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat
     2221 tcgaaggagt gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat
     2281 cgtaaataaa aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca
     2341 ggaaacgaaa ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg
     2401 gaattacgcg gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt
     2461 gaatgccact tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt
     2521 ggaatattcc gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa
     2581 acgtttagat taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa
     2641 aaaacaaaaa ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt
     2701 gaaaaactgg aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct
     2761 ttctcgactg ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga
     2821 aggtgtctac tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg
     2881 gatcgattac tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca
     2941 tacggacatt atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg
     3001 tgaaaaagtc gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
     3061 ttgcataaat ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt
     3121 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     3181 ttttcgtttg gttgggctta ttaccgcaga tacaagcttt tgaacaccgt ttattaagag
     3241 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     3301 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa
     3361 gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc
     3421 tcatattttt cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat
     3481 tgagaagttg tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt
     3541 tcctcatatt ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta
     3601 gtggagaagt tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg
     3661 ttcctcacaa ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact
     3721 ccaaatcaca aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat
     3781 tctatgtttt ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa
     3841 ggaggtttta cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct
     3901 tatagtgctt gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc
     3961 tatacattga tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc
     4021 catcaagaag acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca
     4081 tattttcaaa aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta
     4141 gcgggatgct tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg
     4201 aaaagtagcc gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa
     4261 aatttgaaca tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg
     4321 gaaatcctgc tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact
     4381 ataaagtaaa agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc
     4441 gagtcacaag gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt
     4501 gaccgaggag ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc
     4561 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact
     4621 tattaccgca gatgcaagct tttggacacc gtttacc
//
LOCUS       DAZUKD010000166         4566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000166 DAZUKD010000000
VERSION     DAZUKD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.171"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..310
                     /locus_tag="ACG8N9_002697"
     CDS             14..310
                     /locus_tag="ACG8N9_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898233.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            312..734
                     /locus_tag="ACG8N9_002698"
     CDS             312..734
                     /locus_tag="ACG8N9_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898234.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            753..2345
                     /gene="mobP2"
                     /locus_tag="ACG8N9_002699"
     CDS             753..2345
                     /gene="mobP2"
                     /locus_tag="ACG8N9_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS4898235.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2366..2686
                     /locus_tag="ACG8N9_002700"
                     /pseudo
     CDS             2366..2686
                     /locus_tag="ACG8N9_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2847..3988)
                     /locus_tag="ACG8N9_002701"
     CDS             complement(join(2847..3760,3760..3988))
                     /locus_tag="ACG8N9_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4898236.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            4236..4430
                     /locus_tag="ACG8N9_002702"
     CDS             4236..4430
                     /locus_tag="ACG8N9_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898237.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT         1590 a          743 c          923 g         1310 t
ORIGIN      
        1 ttggaggata tttatgaatg ttaaaattag agatttagat ccaaaattca tttctgaaat
       61 tgatcgaaga tgcgttgaac taagtaatcg tacaggaaat aaatggagtc gtaatgatta
      121 tttaaagcta ctcgttgaaa atgattttga tcgtcctttg atggaataca aacaagaaaa
      181 gtttgaccag ttattagaaa agttttcaga aattcaaagt tataacacaa agatattaga
      241 agaatatgtt agccaaaata atcgtattat agaaatcttg attgagcaaa atagaggaag
      301 tgaactttga tatggcaaag gttatagaca aacacattta ctttcaccaa acacatatcg
      361 atgtattgga tgaaactatt tctactcatg cagaggttaa aaactatagt gaagctgttc
      421 gctttttatg tatgaactat gatccagaag tggagaaaaa agtaaattcc aatcaagcaa
      481 agttgaatgg aatgagtaaa gaaatttcta ttttaaccga aatggtttca gaatttgcgg
      541 atcttcattt gaaagataca ggaatattaa ttggtaaaaa ttcaactgtt tatcaagaag
      601 caaaagaacg agtggaagga aaaattaaat ccaatcaaac aaaaaaatat tcccctcagt
      661 tgaagaaacc tgtgaatgaa tctgttcatg ccagagtaga tccatttgat ccaacgaaga
      721 ttctaaaaag ataaaaatga aggaggataa taatgactca atcgccttca gttattctca
      781 cttgtcaatt tactttgccg aataaaaagt cattttctac ttatattgac tatattacaa
      841 gggaacaggc tcttgaagaa aaggaacgac gtacgcccga agaagaacag gaacttcaac
      901 gaatcaagaa tgctttagag gagttttata ttcctgaagg agaaactttt tcggagagta
      961 agacgggtaa agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa
     1021 tcaattttga caagttagag gaacaagatt ttaccaatta tctttcttat atgacacgtc
     1081 actatgcgtt agaacaaaag aaagaactga cgcaaagtga acaaaaagaa taccaacgct
     1141 tgaaccaagc aatgaaaaaa tatcaaacag aaccccataa aaaagcttca ggaaatcttc
     1201 ctggagtttt ttctatagct tcagacgaag tgaaaggaag tgatctgaag gagattaagc
     1261 aaatcttcca aagaggacaa caaaaaggct caatcattta tcaagatgtt gtttctcatg
     1321 ataattcgta tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag
     1381 aagggttaaa agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga
     1441 atgaaacagg ttactggatg gcaacgattc accgtaacac taaacatatc catattcatt
     1501 ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac ggaggttgaa aatggtgtga
     1561 gttatatcgt accgaaaggt aagagaagac aagcaacatt agacgcaatg aagaccaatt
     1621 atgtacatga gttagaacgt ttttcttatg aaaaggatcg aatgattggt atggagaaac
     1681 gaaatttatt gacgcggaaa agtgatttgc gtaacacctt aacaaaagtg gtaaaagagc
     1741 cagcgcgtta cgatcgacac gcgatgaact tattaaatga agtatataaa accctacctg
     1801 acaaacgttc ggaatggaat tacggggatg aaaaacgaac caaactttca ccatacacaa
     1861 gagagaaact cgatgagtta accaaatatg ttttaacaaa agaacctgaa tatcaagaat
     1921 atatacagat ttcaaaagca ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga
     1981 gagaatcaaa ggatgcagag aaaaatgctc tctttgattt aaaaaagcgc acaggaaatg
     2041 ccatcctaac agagctgaag agacaggata gtcaaataaa accattaata gaaagtgttg
     2101 atcagatgca atctatttac aatgaacgat cctttcgaac gcttaaacaa ttgccaacga
     2161 atgaatacaa atatagttac gaagaattca tgaagcgcag gaaacaattc caacaaagag
     2221 ttgtcacgca gcggagtttg cgtcaattaa atcgaacgat taatgcccat gaagaaaaat
     2281 accacgcaag taaagcatac gaagagcaac aacgaagaat tcaaatggaa caagaaagac
     2341 agtaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga aagttataga
     2401 aggatcatta agagaagaac gaatcgaaca acttcctgat tgtttagaaa aagagcattt
     2461 gttccagata agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt
     2521 tgagtaccca gctactggtg tgtattgttg taatcatgtg aatggtgaaa aatattttat
     2581 cattcaagaa tatgagggca agttagcacc ttattatact acttgggaaa tgaatgaaga
     2641 gggaatcaat aactttccat gtaaaagcat tggatttgag tcaatacctc ggacacgatt
     2701 tgtgagaatt ttttcgagat tccagcgggt gagtatctca tcgtttcgac cagcggtcaa
     2761 accaaaaccg tttgaccgct gatcgaagcg atgaagttga aaccctgtgg aaatctcgcc
     2821 aaaaaaatgt aagtggtagc taggaactaa gcttgcaatg ccaactcctc catctgattt
     2881 ggtgttaaat agttgatact actatggatt cgcttgcgat tgtaaaatcc ttcaatgtat
     2941 tgaaataacg ctaaataagc agcttcaaag ttttcatacg cttttgatgg atagacttct
     3001 tcttttttga gtgaggcgtg aaacgattcg ataccggcat tatcgtaggg acagccctta
     3061 cgactgaagg agtgccgaat gttcaattca ttcagctttg cctcataggc ttcgctagtg
     3121 tactgagaac ccaaatctga gtggatgatc aagttctcgg agggtgtttg gtttaagagc
     3181 gcattgtcaa gggtttcgat aaccaattct ttcgtcatct gcttgccaaa cttccaaccg
     3241 atgactttct ttgtgtggag gtcttgaata gtggagaggt aggtccagcc atcttttagg
     3301 gtatgaatat aggtaatatc tgtcacccat ttttgattga tcgacgtagt ggtaaaatct
     3361 tggtttaaca ggtttttacg tccttcactt ttaactttcg cttgtttgcc aggcttgtac
     3421 ttcttgaggg tgactgaacg caagcctaat tttctcatca gtatttgtac tttctttaag
     3481 gacaaggtct cgccacggcc aacaagaatc ttatgaattt taggcgcacc atagataccc
     3541 ttgttttcaa agtagattcc tgaaatcaag cggtttaagc gtttgttttc tgattggcgc
     3601 ttggaaggtt tctttttctt ccagtcatag tagctggatt tcggtgcttc caaaacggta
     3661 cacatttgtt ttacgtcatg atcatgaaca tttgattgaa taaattcgaa gacatcactc
     3721 atttttactt ttgagcgaat atggttaaag ccttttttag gatagtgttt tcctcctgca
     3781 tgcgggccat ttcctttttc atcgctagga tttctgcctc agtcatgccg gttgctacat
     3841 tctttgtatg aagcttcttc caacgataaa tcgtttgtgt agcgatacca tattgcgctg
     3901 ccagttcttc gaccgattgg ccagactggt ttaaatctaa gatcatcttc ttaaagtctt
     3961 catcaaattt tttcttggct tttgccatcg tggacacgtc cttttcttag tagttagatt
     4021 ctaccaaatc gtgtccatga aatcatccta acatcacatt gaagagtcca tttcactcac
     4081 agagtgttag ttgttaagtg aataaaagaa gtaaaagagt cctgaacgtg attgtccagg
     4141 actctttttt tcaatgaatt gttttccgcc aactcattga gtggtgcgat ttgcgactac
     4201 attatatcaa aagaaagaga agggaggaag cagaaatggt tcattcaacg attggagttg
     4261 ttgttgaaaa aagcagagac aatctcgttt ttgtgacaga aattcaaaca ggcagatctt
     4321 ttgtcgtcac agacaaagcg gcaaaagctt atcaaagtag cgatatttta acgttaaatc
     4381 tgactacgaa gacgttcgtc gatgcgacag aagattatcc atttgtttag tctaatggtg
     4441 ataagtcaag atgaaattta agattttctt tattgagtaa tatgttttga agcgcacgaa
     4501 actagacccg atcaaaatca aatttttttc gggttgattc aaggatttgc gtcaataacg
     4561 gattgt
//
LOCUS       DAZUKD010000167         4420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000167 DAZUKD010000000
VERSION     DAZUKD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.172"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1283)
                     /locus_tag="ACG8N9_002703"
     CDS             complement(<1..1283)
                     /locus_tag="ACG8N9_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFS4898238.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
     gene            1576..2472
                     /locus_tag="ACG8N9_002704"
     CDS             1576..2472
                     /locus_tag="ACG8N9_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4898239.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            2564..2779
                     /locus_tag="ACG8N9_002705"
     CDS             2564..2779
                     /locus_tag="ACG8N9_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4898240.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2797..3069
                     /locus_tag="ACG8N9_002706"
     CDS             2797..3069
                     /locus_tag="ACG8N9_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4898241.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            3071..3934
                     /locus_tag="ACG8N9_002707"
     CDS             3071..3934
                     /locus_tag="ACG8N9_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4898242.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1592 a          748 c          785 g         1295 t
ORIGIN      
        1 attaattgat tgatcaaaaa gattctaatt ctcatgttct caaatgattt aaatccgtag
       61 gatactcgtt tcattgtctt tatatgggta ttcttcgctt ctatttttcc attggtgtaa
      121 ggatagatca tcgcgttggt gatgccttct tcataggtca gaaggttttg gagcttttcc
      181 cgaaagccgt catctaacgt ttcgggaagt tctgccaata aggagaaaaa taagtcaggg
      241 tctttgtctc gaaaggcttc aactaattca tgaaaatagg gatacgcctc cttcagggac
      301 gtagaaaagc caagcaatcg atcaatcatc atcgcttccg taagaaatgg gtattttggt
      361 gctcgaaaac ttttccatgt tttgtattca taatggttga tgtttgcacg attttttagc
      421 aaaaagcgcc agttcttttt cagtttttct gcctgacttt tctgtcctac tttacgaagt
      481 tcattcattt cacggatacg caactcatta aaggcttgat tcatgtgttt gataatatga
      541 aaccgatcaa tcactacttt cgcatttggc agaatacgtt tggtaagctg gaagtaggcg
      601 gcgttcatgt ctgtgactaa aaattttact tcttcaggat tagcgcagcc taagaaatag
      661 cttgttaatc gaggcaattt acgcgtaggc aagacatcaa ttaattgtcc tgtttcgcca
      721 tctgcgcaaa taaagctcat tttatcttct atggaagcgt gcgaacgaaa ttcatcaacc
      781 atcaatactc tggggagaat cctcttagat tgctttggta agtagctttt aaactctttc
      841 aacgtacgaa taacggtggt caaagatacc tgacaatttt tcgcaataaa agataaagat
      901 actttttcag tcagtaaaga agcaatttta tatctgacat gattcgcgat tgaatgtctg
      961 ggttgaacaa aataactttg agccgtccaa tgggttcgac agtttttaca ggtatagcgc
     1021 tgctttttta agcgcataac caaaggcatg tgattgtatt gttcaaagcg gacaatcgtt
     1081 tccttcttcc catttttcac tacaattact ttcccgtttc catctaccac agtagaacca
     1141 caatttctac aggtatgggg agcaggtgag aaaacagcat cgacgatcaa cgtctttttc
     1201 ttctgaaagg tctcgtaaga gacctctgta atcatcaaat ctttctctat taatcgcagc
     1261 atttttttga tagaatcatt catatagcat atcgtcctct cagttgttta ttttgtcgtg
     1321 atttaatcat actagagaac gatatgtttt ttaataccta aaataaaaat ggggctgaag
     1381 aatcaattct gattcatcag tcccataaat tatagagcca agataaatag aggtctttcc
     1441 actactatct aaatggaaag acctctattt ttcatatagg agctactttt gggtcagccc
     1501 tatatattaa tcacaaatca cttatcacaa atcacaagtg attaatcaca aatcacttgt
     1561 gatttgtgat tcttaatgat acaatattac tatacaaaaa aagaatgggg cgtagttatg
     1621 gagaaggaaa aactaaaaat acttgaagaa ttaagacgta ttttaaacaa taaaaatgaa
     1681 gcaattatta tcttgaataa ttactttaaa ggtggtgttg gaaagtccaa attatcgact
     1741 atgtttgctt acttgacaga caaattgaat ttaaaagttt taatgatcga taaggactta
     1801 caggcaacat tgacaaaaga cttagcaaaa acttttgagg tagaattgcc acgtgtcaat
     1861 ttttatgaag gcttgaaaaa tggaaacttg gcttcttcta ttgttcattt gactgataat
     1921 ttagacttga tccctggcac gtttgatttg atgttactgc caaaattaac tcgctcatgg
     1981 acttttgaaa atgaaagtag attgcttgct actcttttag cacctttaaa aagtgactat
     2041 gatctcatta ttattgatac tgtaccaacg ccaagcgttt atacaaataa tgcaatcgtg
     2101 gcgagtgatt acgttatgat ccctttacaa gcagaagaag aaagtacaaa caacattcaa
     2161 aactatattt cctatttgat tgatttacaa gaacaattta accctggact agatatgatc
     2221 ggttttgttc cttatttagt tgatacggac agcgcaacga taaaatcaaa cctggaagaa
     2281 ctgtacaagc aacataaaga agataacttg gttttccaaa atattatcaa gcgaagtaat
     2341 aaagtaagta cctggtctaa aaatggaatt acagaacaca aaggctatga caaaaaagtt
     2401 ttatccatgt atgagaacgt attttttgaa atgcttgagc gaattattca attagaaaac
     2461 gaaaaagaat agaatcacaa atcacaagtg attaatcaca aatcacttgt gatttgtgat
     2521 tgttgatgat aaaataagaa taagaagaaa tagaaagaag tgagtgattg tgggaaattt
     2581 aggcgcacaa aaagcaaaac gaaatgatac accaatcagt gcaaaaaaag atataatggg
     2641 agataagacg gttcgtgttc gtgctgactt acaccatatt ataaaaattg aaacagcaaa
     2701 aaatggcgga aacgtaaaag aagttatgga tcaagcctta gaagaatata tacggaaata
     2761 tttacctgat aaactttaaa aaggagtgaa aatgaaatgg cagttacgta tgaaaaaaca
     2821 tttgaaatag agatcattaa cgaattatcg gcaagcgttt ataatcgagt attaaactat
     2881 gttttgaacc atgaactaga tactaaaaat actcgtttac tagaagtgaa tcttttaaat
     2941 caattagaag tggcacaaga agttgattta tttcaacaac catttgaaga attacaagct
     3001 attcatgagt attggcggtc aatgaatcaa tattcaaaac aaattttgac aaaagagaaa
     3061 gtggcttaac atggcgaata ttactgactt caccgaaaag caatttgaag atcgtttaga
     3121 aaagaatgtt gaacgactaa ctaaaaatag actagcggtt gaatcgccaa ccgctttttt
     3181 acttggtggg caaccaggat caggtaaaac tagtttgcga tcggcaattt ccgaagagac
     3241 acaaggaaat gttgttatca ttgataatga tacgttcaaa caacagcacc ctaattttga
     3301 tgaactagtg aaactttatg aaaaagacgt agtaaaatac gttacccctt attctaatcg
     3361 catgacagaa gcgatcataa gccgtttgag agataaaggg tataatttag tgattgaagg
     3421 tacaggacga acaacagacg ttcctattca aaccgcaaca atgcttcaag ccaaagatta
     3481 tgaaacaaaa atgtatgtca tggcagtacc taaaatcaac tcatatttag gaacaattga
     3541 acggtatgaa actatgtatg cagatgatcc aatgacagcc agggcaacac caaaacaagc
     3601 gcatgatatt gttgtcaaaa acttaccgac caatttagaa acccttcata aaacgggctt
     3661 atttagcgat ataaggcttt acaatagaga aggagtgaaa ctctattcaa gcttagaaac
     3721 gccttccatt agtccgaaag agaccttaga aagagaatta aatcgtaaag tatcagggaa
     3781 agaaattcaa ccgactttgg agagaataga gcaaaaaatg gttcaaaatc aacaccaaga
     3841 aacccctgaa tttaaagcaa ttcaacaaaa aatggaaagc ttgcagccac ctacaccacc
     3901 aatacccaaa acacctaaac ttccagggat ttaaacctta aaaaagtaaa agagtagtta
     3961 ccaaaaaacg gtaactactc tttttttata aaaacatttc ctcatttttt aaaagctgat
     4021 cgagaaataa agcacacaac tgtattcttt cttttttcag ttggtacatt ttgcagtctg
     4081 taacgtgcca tttactgttt gttaccttaa catatccagt cgaaaacaca agggtatata
     4141 aacgagcaag ctcgcccttg tgttttcttt gtgtctatgt tgcgttctcg tcacagcaaa
     4201 cgacacgaac acactggcga aaatgaacca acaaaacgaa aaaagaaagg agcgtacact
     4261 gtaatttatt tctagctttt aaaacatgag gaaaacagct tcacttcctc aaacgcctaa
     4321 caaaaatggt tgtcaaaaaa ctaaaaatca aaaaaagaaa agaggaaaag aaaatggaaa
     4381 aaacaaaaaa attacaatta gaagatttta cagaaaatgg
//
LOCUS       DAZUKD010000168         4409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000168 DAZUKD010000000
VERSION     DAZUKD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.173"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..403
                     /locus_tag="ACG8N9_002708"
     CDS             <1..403
                     /locus_tag="ACG8N9_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4898243.1"
                     /translation="YYRQQNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKA
                     CMLVAFDVLIMEIFNQKNVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNM
                     RKKLALVTISIMFNFKMYKTAYNMLWRIYNR"
     gene            417..1496
                     /locus_tag="ACG8N9_002709"
     CDS             417..1496
                     /locus_tag="ACG8N9_002709"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4898244.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            1515..2939
                     /gene="wzy"
                     /locus_tag="ACG8N9_002710"
     CDS             1515..2939
                     /gene="wzy"
                     /locus_tag="ACG8N9_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS4898245.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            2985..>4409
                     /locus_tag="ACG8N9_002711"
     CDS             2985..>4409
                     /locus_tag="ACG8N9_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4898246.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWARKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICI"
BASE COUNT         1536 a          547 c          800 g         1526 t
ORIGIN      
        1 ttattatcgc caacaaaata attctataat gcataagcca aaaagtaatt tggtttttag
       61 acatggaaat atgattgctg attatgaaaa gttgataagg caaaatggaa acaacataat
      121 agataaaaaa gcttgtatgt tggttgcatt tgatgtacta attatggaga tttttaatca
      181 aaaaaacgtt ggtggaacat gtgaagatta taagaatgca ttagtttctt tgaaacatca
      241 taaaatattg agagaaaata tttcaaatat agttagaatg aaagcatatg aacttgaacc
      301 taatatgaga aagaagttag cccttgtgac cattagtata atgtttaatt ttaaaatgta
      361 taaaacagcg tataatatgc tctggagaat atataatcga taaagaggta ggaaatatga
      421 ggaaattgac ctttgtgata ggaattatgg gaaaaggagg ggcagaacga gtaattgcga
      481 atttggcaaa taattttatt caagataact gggaaattga tattataact atatatggga
      541 atcggcaaga ctatgaactg aatagaaaaa tacaggtaca tccaataata tgtaaaagtt
      601 cgtttcgtgc atttagacct ttggaacgct ttaaggaact taggaaaaaa ataagggaaa
      661 tacagcctga tctggttatt tcgttccttg ctgatgtaaa tatccatgtc attatttcat
      721 tgttaggagt aaaacaaaaa ttaattgttt ctgaacgaaa tgatccacaa cggaatccag
      781 aagaaccatg gattaggaaa attagagatt ttctttataa aaatgtagat ggaattgttt
      841 ttcaaacacc agacgcaaaa aaatatttta gtaagatcaa tgataaaaaa gtaaaaacaa
      901 ttataattcc caacccaata aaggaaaatt taccttatta taataaaact acaagtagta
      961 aaaaatttat tactgcatgt agattggata aacaaaaaaa tttgccctta atgattgatg
     1021 caataggaaa acttattcat gaaggatatg agtgtgaact ggatatttat ggagaagggg
     1081 gattaaaaac cgagcttgaa agttatatca accataatgg attaacagaa tttgttcatc
     1141 taaaagaatt tacaaataaa atacatgaaa agatgcaaaa tgcggcagga tttgtaattt
     1201 cttctgatta tgaaggtata tctaattcta tgttagaagc actggctatt ggagtgccag
     1261 ttatcgctac agattgtcca gtaggtggcg caagaatgta tttaggtgat agaaatgaaa
     1321 taggtcatct tgttgaatgt aataatctag ataagttaac agatgcaatg cgtagggtac
     1381 ttttggatag agaaagtgtg gtaagaaaag cgcaaaaagg aattaaacta agaaaagaac
     1441 tgacatctaa aaatatttca aatatatggt acgaatattt aaaccagttc ttttaaccaa
     1501 aaggaggata ttgtgtgaaa gaaaaaataa atatattgat gttttcgtta acgttagtcg
     1561 cgtttacaat attttgcgct ttttcattaa atcagaacta ttggctaaat tggcttggaa
     1621 tattgattta cttatatgca atctgtacat ggcattggag aaaaggagat tcaatatttt
     1681 cattatacac gatttttttc actctttttt tattatttag ttatgggcaa tgcattatgt
     1741 gggcattcgg tattggaaca gatcgtggaa tcggtacaac agtagtagct tatggtacgg
     1801 ggattattcc tagtgaatca gatatgataa tggtaaaatg gtattcttgt atttctatgt
     1861 ttgtatttca cataggagca ctcttattta cgagaaaaac cacactaaga aaagttagtt
     1921 ggtatgaatc tggagatgct gatgccgata gaaagttttt atttaaaata gggtgtatca
     1981 tttctataat agtagtaccg atagtttttg tttctaaatt actagaggta cgtatagcat
     2041 tagtccatgg ttataatgca ttatattatg gtgattatgc tacacaaagt ggatatttac
     2101 aaattattct atatcttttt tttccagcct taatatgttt attaattggt agcaactttt
     2161 caaaagtaat aacgagattt gtttttatta tttttgcttt atattcttta ttaggaatta
     2221 tgtcaggaga cagaggaagt tggttgtata gctctattat actcatatgg gcatacactc
     2281 aatataaagg aacctttaat tataaaaaat taataaagtg gcttatttta gctgttatag
     2341 gaatatatat tctaaacgta ataacgaaag cacgtgatgg tggaggacta agtaaactta
     2401 ctttaaaaga ttttacatct gtatttgatt cagatgattc tcctttagtt gattcttttt
     2461 ttgaaatggg ggggactatg agtattataa cttttttcct aataagtggt aatggaatat
     2521 atccctttgc taatacttat ttgacctcac tcttaggctc tatatcaaca agaatgctat
     2581 caatgtttgg aataaagttt gttttattag ctgattggtt ttctcaggat tatttaggaa
     2641 tcacttgggg gactggattc tcaatgattg cagaagccta tgtcaatggt ggatacattg
     2701 ggggtatagt ctatattttt attattggta tagtcatcgg taaaatcctt tctaatagta
     2761 tagacaacca agatattgaa aatcatccta tgaagatgtt tttggctata actacagcaa
     2821 atattataat gggttttcct agagctgctt catatttaat tgtgaaaaat tttgtgtatg
     2881 gtgttttaat tatattgttg ataaactttt tcctacagca atttaataaa cgtctttaat
     2941 atttaagctt aataaaaaga taaaacaagt caggagttgc aaaaatggaa agaactgctc
     3001 ggctcaaaaa aaacactatt tgggctctgg tgtttcaaat aacttctatt gtctgtggta
     3061 tgatcttgct acgtttgata ttaacaaagt acggatccga ggttaatgga atgattagct
     3121 ctattaacca atttctacaa attatttctt ttttacaatt aggagtaggc gctgtaatgc
     3181 aatcaactct atacaaacca ctagcagaaa aggataattt aatgataagt caaatcttag
     3241 cttctggagg gaagttttat cggcaaattg catatattct actagtatac gtgggtttgt
     3301 tacttgcagt ctattatgtt ctcgttccta atgaatttgg gagatcatat acaatggcaa
     3361 tgattgcaac attaagtatt aattcatttg ctcaatatta ttttggtatt gttaatactt
     3421 tgttattaag tgctgaccag aaaatttata ttacgtatat tgcacagaca atagcattta
     3481 tttctaatac agttgcatgt gctttattaa ttagtctgaa cacagagatc cttatggtaa
     3541 aacttattac tgctataatt tatcttgctt gtccaatata ttatagatgg tatgtggatc
     3601 gaaactataa tataaagagg aatgtgtcat atgataagga accgatccct caaaaatgga
     3661 atggggttgc acaacacgta gcttctgttg tactagatag tactgatgtt gtagtgttat
     3721 ctatattttc taacctgata tatgtatcta tatataatgt gtacaattta gtaatatacg
     3781 gagtaaaaca gctattcatg gctatgacaa atggcgttca ggccttacta ggggaacttt
     3841 gggccagaaa agaaatagaa gttctttaca aaacatttgc atggttcgaa tggattgtac
     3901 atactttgac agtttttatt tttggctgta ccggtatact aatcgttcca tttattcaag
     3961 tatatacaag aggagtaaca gatgctaatt ataatcaatt atcattcgca gctttacttg
     4021 taactgctaa cgcacttcac tgtctgcgta caccttatca tgtaatgata aaagcagctg
     4081 gtaaatataa agaaactcaa aatagtttta taattgcttc tttgttaaat ttaggtttat
     4141 cattaatttt ggttaaacca ctagggctag ttggagtagc attaggatct ctaatttcaa
     4201 tcacatatca tacactttgg agtgcaaatt ataattcaaa atatttgctt aaatggccga
     4261 tgaaaaaatt ctataaacag ttatttgtaa acatcttaac tgtagttctt tcttttgggg
     4321 cgacatcaca attgaaaatg atgtcaatca catatttgga ttggattgtt ctcgcaatta
     4381 aatgtgcagt tattgtattt atatgtata
//
LOCUS       DAZUKD010000169         4396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000169 DAZUKD010000000
VERSION     DAZUKD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.174"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACG8N9_002712"
     CDS             <1..790
                     /locus_tag="ACG8N9_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4898247.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="ACG8N9_002713"
     CDS             887..3136
                     /locus_tag="ACG8N9_002713"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4898248.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..4314
                     /locus_tag="ACG8N9_002714"
     CDS             3139..4314
                     /locus_tag="ACG8N9_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4898249.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1535 a          630 c          807 g         1424 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact agagtaaccc gtggtgctag ttaattttaa
     4321 tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca
     4381 ctgtaagcca gtaata
//
LOCUS       DAZUKD010000170         4343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000170 DAZUKD010000000
VERSION     DAZUKD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.175"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACG8N9_002715"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACG8N9_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACG8N9_002716"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACG8N9_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACG8N9_002717"
     CDS             1898..2059
                     /locus_tag="ACG8N9_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898250.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2543..3139
                     /locus_tag="ACG8N9_002718"
     CDS             2543..3139
                     /locus_tag="ACG8N9_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS4898251.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            3120..3566
                     /locus_tag="ACG8N9_002719"
     CDS             3120..3566
                     /locus_tag="ACG8N9_002719"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4898252.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            3909..4232
                     /gene="relB"
                     /locus_tag="ACG8N9_002720"
     CDS             3909..4232
                     /gene="relB"
                     /locus_tag="ACG8N9_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4898253.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
BASE COUNT         1597 a          614 c          746 g         1386 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     2461 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     2521 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     2581 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     2641 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     2701 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     2761 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     2821 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     2881 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     2941 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     3001 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
     3061 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaatcaag agcaattgat
     3121 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
     3181 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
     3241 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
     3301 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
     3361 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
     3421 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     3481 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     3541 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     3601 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     3661 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaatt
     3721 ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt agttggctac
     3781 ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat ttactacaag
     3841 gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa aatcacatca
     3901 ggaaatgcat gagattcttt aagagaggca attatagggc tatgattacg gttcgtgtaa
     3961 gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt actttatctg
     4021 aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt caaacggctg
     4081 aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg gaagaatcct
     4141 taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga aattcagaag
     4201 cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat tcgttgttat
     4261 cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt atttttacaa
     4321 aaaaataata attggaatct agt
//
LOCUS       DAZUKD010000171         4255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000171 DAZUKD010000000
VERSION     DAZUKD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.177"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(300..914)
                     /locus_tag="ACG8N9_002721"
     CDS             complement(300..914)
                     /locus_tag="ACG8N9_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4898254.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1520..1897)
                     /locus_tag="ACG8N9_002722"
     CDS             complement(1520..1897)
                     /locus_tag="ACG8N9_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898255.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2047..2172)
                     /locus_tag="ACG8N9_002723"
     CDS             complement(2047..2172)
                     /locus_tag="ACG8N9_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898256.1"
                     /translation="MKGYLLLKRQLVRHLRNCRFSFLTLDQLSRGKRKIEKDFRE"
     gene            complement(2197..3078)
                     /gene="galU"
                     /locus_tag="ACG8N9_002724"
     CDS             complement(2197..3078)
                     /gene="galU"
                     /locus_tag="ACG8N9_002724"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4898257.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3121..4140)
                     /locus_tag="ACG8N9_002725"
     CDS             complement(3121..4140)
                     /locus_tag="ACG8N9_002725"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4898258.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         1244 a          909 c          661 g         1441 t
ORIGIN      
        1 tgtattaaga gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt
       61 tttatttttc tgacactaca tattcttata actcatttct cataactgtg aaacaactta
      121 taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata
      181 actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa
      241 acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac
      301 tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc
      361 aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc
      421 aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat
      481 cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg
      541 attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt
      601 ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt
      661 gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata
      721 cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg
      781 ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct
      841 tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa
      901 taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc
      961 ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct
     1021 gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa
     1081 acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt
     1141 acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg
     1201 tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta
     1261 ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa
     1321 atacaaaaaa acattggtat gatttatata tatataaata acataaaaaa aggaatggct
     1381 tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt
     1441 ttttccacca attaaataaa tctctcatct caatagttta tccattattt tatctttttt
     1501 tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat
     1561 ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca
     1621 tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt
     1681 cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc
     1741 agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa
     1801 taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat
     1861 caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat
     1921 aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct
     1981 tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt
     2041 ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag
     2101 agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa
     2161 atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt
     2221 actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata
     2281 ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa
     2341 ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat
     2401 ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag
     2461 ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa
     2521 cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt
     2581 agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata
     2641 atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat
     2701 gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc
     2761 tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc
     2821 gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa
     2881 gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga
     2941 atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc
     3001 ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac
     3061 tgcttttttg accttcactt gctgttcctc ctaaacgacg attaactatt gatcatctat
     3121 ttaacgaaga gaaaattcat tctccatttt tccgtcacgt aacatgattt cttttgctgc
     3181 ttttttgata tcttctccat tatacaaaac attatagatt gtttgagtaa ttggcatctc
     3241 aacacctagc tgttgagcta gctctactgc tgcttttgtc gttgccacac cctcaacgac
     3301 cattcccatg ttttctagaa cttcttccag cttatgtccc tgtcctaaca agttaccggc
     3361 acgccaattg cgagaatgaa cacttgtaca agtaacgatc aagtccccca caccactcaa
     3421 accgataaat gtcagaggat tggcacccat cgcgacacca agacggctga tttctgccaa
     3481 tcctcgagtc atgattgctg cttttgcatc atctccgaat cctaatccat ggatagcgcc
     3541 ggcaccaatt gcaatgatat ttttcagtgc tgcaccagtt tctacgccaa tcacatcatt
     3601 atttgtataa atcctgaaat aatcattcat gaacagtttt tgtacatagg ctgctgcttt
     3661 ttgatcaata cttgcagctg taattgttgt gatatcatgt actgccactt cctcagcatg
     3721 gctaggacca gataatacaa caattgcctg tcttttttct tctggaattt cttcagccaa
     3781 aacttctgag attcgtttat gcgtatcttg ttctagtcct ttacttgcat gtataatgat
     3841 tggcttagta ttcattttac caataagttc ctgtgcgact gaacgaatcg cttttgttgg
     3901 aacaacaaat aaaatagcat ctgcgttatc cactgcttct gccaattctt tatatccgat
     3961 aatcgattct gggatcgtca aatcaggtaa aaaatgctga tttgtatgat acttattgat
     4021 ttcatcaatc tgttcaggta catttcccca aatacggaca tcatgcccgt tttctgccaa
     4081 tgtttgtgct aaagcagtgc cccaagatcc aggaccaaga actgcgattt tttgtttcat
     4141 ggatattcct ccaaataaat aattgataat ttccattata gtatacattt cttaaagttg
     4201 ggactaattt ccattgtttt taagtgtttg tttacttctt ttatcgtttt tactt
//
LOCUS       DAZUKD010000172         4249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000172 DAZUKD010000000
VERSION     DAZUKD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.178"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..951
                     /locus_tag="ACG8N9_002726"
     CDS             148..951
                     /locus_tag="ACG8N9_002726"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4898259.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            984..1838
                     /locus_tag="ACG8N9_002727"
     CDS             984..1838
                     /locus_tag="ACG8N9_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFS4898260.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            1835..2536
                     /locus_tag="ACG8N9_002728"
     CDS             1835..2536
                     /locus_tag="ACG8N9_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS4898261.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            2544..3611
                     /locus_tag="ACG8N9_002729"
     CDS             2544..3611
                     /locus_tag="ACG8N9_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4898262.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            3604..>4249
                     /locus_tag="ACG8N9_002730"
     CDS             3604..>4249
                     /locus_tag="ACG8N9_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4898263.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKS"
BASE COUNT         1541 a          609 c          840 g         1259 t
ORIGIN      
        1 ataagatgtc atctcaagga ctagatgaag atgaaaatat tagttcatta gcagaaatcg
       61 acttttcaca agagaaaatt tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag
      121 agaacattgg aaaataaaaa tgtaagtatg ctgctttctt tatatgataa agaaaagcca
      181 gagtatttaa aggaagcatt aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt
      241 gtattagttt atgacggccc tataaatgaa aaacttcaag tagttgtcac ttatttccaa
      301 caaaaagatc ctcacttgtt tacagttgta aaattagaaa aaaatcaagg attggggatt
      361 gctttagcaa ttggattaga acatacgaaa aatcgcttag tagccagaat ggatacagat
      421 gatataatgg aaccccaacg attagaaaaa caattagaga tcttccaaaa agatccagaa
      481 atagcgattg ttgggagtaa tattgaggaa tttactggca cgcttagcaa tgttatcgga
      541 aaaagaattg tacctgaata taacaatgca attcgtgtat tttctaaaag aagaaatccc
      601 tttaatcata tgaccgttat gtttgataaa aaagctattt tggaagtggg aaattaccaa
      661 ccattaagtg ggtttgaaga ttattatctt tgggcaagat tattaaaggc aggttataaa
      721 ggatataata ttcaagaaac attagtctat gcgcgaactg gcaaagatat gtatgcaaga
      781 agaggcggaa tgaaatatct tggttctggt cttatgggtc gttatcgtat ctggaaagac
      841 ggtttagggt cagtaaaaga ttttataatt gtttcttgcg gacatatttt tattagctta
      901 ctaccaaatc aattaagagg aaaattttat caaacgcaat taagaaaata ggaggttttt
      961 ctttgtatag cactgatcca aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc
     1021 aagagattgt tgatttttgt ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg
     1081 ccatcggtac tgtgagagaa caagggttta ttccatggga tgatgattta gatttcttta
     1141 tgcctagaga ggattatgaa aaattttatc aactatgggt taattcagaa aaaagtaaaa
     1201 aatatcccat acagaaggct tctaaaaatt ataatgatca taattctttt acaactatcc
     1261 gcgatgcaga tactacattt attaaaactt atcaaagaga tttagatata gtccatggaa
     1321 ttactattga tattttcccg ttagatgttg cccctgtttc gaaatttagc cgtaaaattc
     1381 aaaaactatg ggctttagta tatgctttat tttgttctca agtcgtccct gaaaaacatg
     1441 gtggaataat ggctacaggt agtaagatac tcttatctat tttccgctca cctcgtacga
     1501 gatataagat atggtctttt gctgaaaaac aaatgacaaa atataataat gaaccaacgg
     1561 gctatgtgac tgaactttgt gtcggaccta aatacatggg aaatgtttat cctgcaaaag
     1621 actttgaatc tgctgtgtgg gtgccattcg aagatacaga gatgccagta ccagttggtt
     1681 atgataatta tttaagtcaa gtttttggtg actatatgca gcttccacct gaaaaagatc
     1741 aggtttctca tcatgaagca gtttatattg atccagaaca tccatacaca atgtacaaag
     1801 gaaaatatta tttaactaaa ggagcagaaa aaaaatgatt acagctatta ttattgctgg
     1861 tggtgtgggt aaacgaatgg gacaagatat cccaaaacag ttcattacaa ttgaaggaaa
     1921 acctattatt atctatactt tagaatcatt tcaacaccat cctcaagttg accggatttt
     1981 agtcgtttgc aaatctggtt gggaagaaac gatgtgggcg tatatcaaag aattcaatat
     2041 tagcaaagtc aaatgggtca ttacaggtgg atcaaaggga caagagtcga tcaataatgg
     2101 tgttcagttt ttgaaagact attctgaaga tgatgatacg atcatcatcc atgatggaat
     2161 ccgtccacta gtagacgaac tggtattgtc agatgtaatc gttaaatgta aagaatttgg
     2221 taacggtgta acctctctac cttacaacga acaaattttt atcaagaaaa cagaagaaac
     2281 gacggaacag tatgtcgatc gtaacacatt gaggcgtgtc tcaactccac aagcttatca
     2341 atacggaaaa ctacgggcag catatgaccg ggcagtgaaa gaagacatcg gtatgactga
     2401 ttcttcttat acgaatacca tgatggtaga tttgggagaa actttatatt ttgctgctgg
     2461 ttctgataaa aatatcaaat tgacaacaac agatgatcta gaattgttca aagcatattt
     2521 aaaaatgaaa gactaggtga aagatgatga cgttgattaa acaaccaatg taccaagaag
     2581 atttggcaaa agtaatcaat catacaccaa aaataaaaca gctacatgga aaatccgttc
     2641 tgatgattgg tgcttctgga atgatcggct cttttttgat tgatacgttg atggctgcta
     2701 atgaacaact aggcatcaaa atcaaagttt atgcaatggg cagaaaccgt cagaagcttg
     2761 aaaaaaggtt tgccagttat cttgaattag atactttcga aattgttgaa ggtgacgtca
     2821 cagaaccctt accaaaagaa attcaagctg actatttgat acacggtgca agtaacacac
     2881 atcctaaggc ttacgcaact gatccaatcg gtacgatcat gactaatctt gcaggtacag
     2941 agcaagtatt gaaacatgca gtagcaaccc aaccggttag agtattattt ttatcaacag
     3001 tagaaatcta tggcgaaaat cgaggcgata tagaaaaatt cacagaagat tactgtggat
     3061 acatcgattg taatacactg agagcaggct accctgaagg aaaaagagtc agtgaatcat
     3121 tatgccaagc ctacatcgca aaacacgacc tcgacatcgt gattccaagg ctttgccgta
     3181 cgtttggtcc gacgatgctt ttatctgata cgaaagcatc ctcacaattt attttaaatg
     3241 cagtcgaaca aaaagatatt gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat
     3301 atgttggaga tgctgtttct gctattcttc atttactgct tgaaggagaa aaaggagagg
     3361 cgtacaacgt ttcttcagaa aactttgatt tacgactaaa agaattagcc caacagctag
     3421 ctgatatcag cggcaaacaa gtaatttttg aattgccgga tgagattgaa caaaaaggtt
     3481 tctcaaaagc tagcacagca gtacttgaaa ataaaaaaat taaagagaca ggttggaaac
     3541 caatttttcc tttgaacgaa tcattggttc atacagtaga attagttaaa ggtgagaaat
     3601 ataatgtgtg agattagtat tattgttcct gtgtataaag ttgaaaaata tttaagaaaa
     3661 tgtgtagatt cgattttagc ccaaacattt actgattttg aagttatatt agtagatgat
     3721 ggatctcctg acaatagcgg gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta
     3781 cgtgtgatcc ataaagaaaa cggcggatta agcagtgcta gaaatgcggg gattgatgtt
     3841 gcgagaggca aatacttagg ctttgttgac agtgatgatt atattgatga agatatgtat
     3901 gaaattttgt atgagaattt gaaaattcat gatgccgaca ttagctctgt tgaattgatt
     3961 cctttttatg gtgatagata taagaaagct aataaagaaa agaaagtaat tatattaaat
     4021 aaaaaggaag caataaaatc tgttttagaa ggaacgcaat tttatgcata cgcatggaat
     4081 aaactttatc gtaaggaact ttttaaagat aatcgttact tggatggaaa aacatttgag
     4141 gatgcatata taataataga tctattattt caaacagaaa aaattgttgt ttccaatgaa
     4201 gagaaatatt tctatcttca aagaaatgat agtattatgg gaaaaagtt
//
LOCUS       DAZUKD010000173         4152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000173 DAZUKD010000000
VERSION     DAZUKD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.179"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1364
                     /locus_tag="ACG8N9_002731"
     CDS             63..1364
                     /locus_tag="ACG8N9_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4898264.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1391..1651
                     /locus_tag="ACG8N9_002732"
     CDS             1391..1651
                     /locus_tag="ACG8N9_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4898265.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2650..3885)
                     /locus_tag="ACG8N9_002733"
     CDS             complement(2650..3885)
                     /locus_tag="ACG8N9_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4898266.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1196 a          672 c          862 g         1422 t
ORIGIN      
        1 gaacattctt tgcgttgtca tgcaatgaat ggttcatatt ttattgaaaa ggagtggttt
       61 tagtggaaaa gctgatgaaa tgggtcgaag agaaacttgt cccaccaatg gcaaaaattg
      121 gtacacagcg tcatttatta gcaattcgta atggggttgt ttcaacattg tcattgattt
      181 tgattgggac attttttatg gtctttatca atttaccgtt tcctggatgg aatgaattta
      241 ttgcccctta ttcggcaacg attgttttac cttttaggat gacgatggga ttgatggcta
      301 tttatgcaac ttttgttatg ggttcggatc tagctaaatc atatggatta gatagtgtga
      361 caggtggtat attatcttta ggaacgttct ttatgttaca agttcctgta aacgttttaa
      421 ctcctgaaaa ggcacctctt ggctgggtct tacctatgag ttctttggga gcatctggga
      481 tgtttgcggg gatcttatct atgatttttg cagtggaagt atatcgtttc tttaaaaatc
      541 gaaatattac tatcaaaatg ccagaacaag tgccacctgc tgttgcccgt agttttgagg
      601 cattgattcc aggtgcagtc attttaacaa ttacttggct gattcgaagc gtcgcgggct
      661 ttgatgtaaa cgctacctta ttatcgttat tcgagccttt aaccaatatt ttagggaata
      721 atttgcttgg tgttttacta ccaatgtttt tgatccattt gttgtggtca tttggtatcc
      781 atggcatgag tattgtaggg gctgttgttc gtccaatgtg gctgatcatg ttggatgaga
      841 atgcaaaggc attggcagat ggtacagctg ccacccaatt accctatatc gctcctgaac
      901 agttttatca atggacggta acaattggtg gtgcaggagc aacgatcgtg gtatctgttt
      961 tgtttatatt cttctgtaaa tcaaaattct tgaaagaggt tggtcgtttt tctattattc
     1021 ctggtatctt caatatcaat gagcccatga tttttggagc tcctatgatg ttgaatcctt
     1081 acatgttcat cccgttcaac ttagttcctt tagttttgac tattatcagt tattttgcag
     1141 tcaaattaga aatggtcaat agatttactg ttttgccaac ttggacatta ccagctccaa
     1201 tcggcggtta tctttcagca ggaaatgact ggcgtgttgt tatcttgatc atgatcaaca
     1261 cattgatagc atttattatc tattatccat ttgtaaaagc ttatgataaa aagatgctgt
     1321 cagatgaaca aagtcgtgga gaaaacgaag tccaaactgt ctaagtttgg tttttagaag
     1381 gtgggaaaca atgtattggc tgattttatt ttttgttttt atctttttat taacggctag
     1441 tcaccttata ttgaatatgc tagctacata tcatattcaa atcaatcgat ggatttgggc
     1501 actggcatca tttttgatcg tgattcttcc aaagatcatt gtaccgcata tgaatgtttt
     1561 gttttcatgg gggacatatg tactttgtgg aatttttgct atcaatttta tgatcgagca
     1621 gcatagatgg tttgtcacat cgaagcttta aatgttctaa aagtggttgt gacagaagtg
     1681 gttagcttca agaaataaga agaaatttac gaaaattgct tcttaaattt ttgtgaaatg
     1741 ttggcttatt tttcgtttgg ttgaacttat taccgcagat gcaagctttt gaccaccgtt
     1801 tattcggaat atgggactgt gacaaggctt ttgtcacagc ccttttttac ataaagatta
     1861 ttattgtttt aactgttttg gcagaaagaa ggaggcaaaa atgaaaattt tatcagcaat
     1921 ggattaagaa ttagtcgatc tactaccaaa gtgatcaagg caaatataga tttgggagct
     1981 tacaaccata tagtaggggc tgcagtatgg tgtatgaatg gtaatatgta tgatgagagt
     2041 gtttattcaa atcatgaaac gtgtatagaa ataattgcgc tgggaatagc aatcagtcat
     2101 ggtgaacgtg acttttagtt tatcgttgct gacgacatag aacattctga catgatctac
     2161 tcagcatgtg gggattgctg gcagtttata taggagtatg catcggattg cgagatcatt
     2221 gtctcaacag aagagggagg ttgattatca aagataaagt ggtaaatcct acatttgatt
     2281 tagtaattgt tgaagggact agcagcacaa atctctacaa agcgatgctt ctagatctat
     2341 atgacacatt ttaagaaaat gtatttgcct aattattatg gtctttcttt tgaagaaacg
     2401 ttgagatgat ccaaccagag aaaggtgtca aagaatttga ctaagaataa atggcagacg
     2461 ctacctatgc tcatttggat tgcgtcagat cttttatttt aaggatgatt ttgtgttttt
     2521 tagacaccgt aatttaagta actctaactt ttcaatccac tatttacttt ttttgtattt
     2581 tcactgtctg ggtctcgaat agcgaaatta ctgatgagtc tctattaaaa aagctttttc
     2641 tacttgaaac tatattgggt ggttgctcag gaaggtttcg atttcttgaa ttgcttgttg
     2701 tggaccattt attggaaaaa cggcttgcag cgtgcgtgcc tgctgacgaa tcttgggtga
     2761 agaaacttcg cgcagagcaa tctgcagttt tctttcagtc actggttttg tgagggcgat
     2821 tgcgtttccg tattttacac aatagtcaat attacagctt tgctcaaatt gcatccccac
     2881 accgataaac ggtttgccgg attcacaggc agtttggacg gtcccttccc cgccgtggat
     2941 aaaagaaaaa tcaatgaatt catgcaaaag atgactagac agatattctg ttattaaaag
     3001 atcttctgag tcagattgaa tgtcatcaag cagatagtca ataggtgcaa taaccgctag
     3061 atccggttgt ttttgtaaga agaacaaaat tttgcgtaac atacgcggat tgcctgaact
     3121 acccattgcg aaatagataa tacgttttct ttcttgttgc tttcgctgta taaaagttag
     3181 cacttcttca ggcaaggaag tactgagctt agcaaaaatt ggaccaacga ttcgataatt
     3241 ttccggcagc tgttcttctt ccaaaaatag tttaggcgtt gtgatgagat ttagatcagc
     3301 atccatcatt tgtaaacttg tttttggtaa aggaagcatt tctttttgtg cagcgtgttt
     3361 gaaggcattg gggatccatc gggtatttac tatacgcttt tttgtccagt gccaaagggg
     3421 attgcttaaa gggccgaaga aacgtaactc gtggggcatc agcgcatgct ctgattgaaa
     3481 aaaaggttca ctcaaagcat agggtttaac atatactaaa ggaatctttt tattgcgggc
     3541 cgataaaaaa attgtcacat tcgaacctgt cacaatcagt cgaggctcaa acttagcaat
     3601 cagactctgt tcactagtga tacgagaggc aaccatttct ttggtaaaag ggtttttaag
     3661 tgtacgcaac tggtcaaatt ttatgatttg ttcttcttgt tttttcgtaa ggatcggcgc
     3721 tagtaaatga tactcaaagc cactttctgt gatcaaagga gcaaaagttt tagaaaagcc
     3781 aaagaataca cagtaataac ggtctttcat agctttggca acctcaatca ttcgtgttgt
     3841 ctcagctaaa ttgaatactg caggagcaaa aagaattgtc ttcatgaaat cctccttatt
     3901 ggaatgagtc tatcccttcg tttcttacta gtatactaaa aaacacagcg ttcgagtagc
     3961 ctatgtatat tttgaatatt aaactgtctc tcgctgtttt cacaacttct aaatttaaat
     4021 tttgtaatca agttgaaact ccaattttca aaaaaacacg caacaatttt ttgatcgatt
     4081 tatatgttgt gccagataaa tacgagaagt tgtgtcatta aaaataaaca tgagaacata
     4141 aaaaataaaa ta
//
LOCUS       DAZUKD010000174         4150 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000174 DAZUKD010000000
VERSION     DAZUKD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.180"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1481
                     /locus_tag="ACG8N9_002734"
     CDS             21..1481
                     /locus_tag="ACG8N9_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4898267.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            1549..1770
                     /locus_tag="ACG8N9_002735"
     CDS             1549..1770
                     /locus_tag="ACG8N9_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898268.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            1801..1983
                     /locus_tag="ACG8N9_002736"
     CDS             1801..1983
                     /locus_tag="ACG8N9_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4898269.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            1983..2396
                     /locus_tag="ACG8N9_002737"
     CDS             1983..2396
                     /locus_tag="ACG8N9_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4898270.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            2519..3700
                     /locus_tag="ACG8N9_002738"
     CDS             2519..3700
                     /locus_tag="ACG8N9_002738"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898271.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(3771..3935)
                     /locus_tag="ACG8N9_002739"
     CDS             complement(3771..3935)
                     /locus_tag="ACG8N9_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898272.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
BASE COUNT         1289 a          709 c          890 g         1262 t
ORIGIN      
        1 cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc
       61 cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg
      121 gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg
      181 atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct
      241 ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa
      301 tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa
      361 atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc
      421 cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata
      481 gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc
      541 taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt
      601 cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag
      661 taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg
      721 tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt
      781 tggtgcaaaa atatattgca gaaccattcc taggattatc tcaagggttg ggagcagtat
      841 tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga ccaaatgtat
      901 tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc
      961 aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa
     1021 aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg
     1081 gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag
     1141 tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc
     1201 tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa
     1261 cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca
     1321 catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac
     1381 cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct
     1441 ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg
     1501 acgctttaag tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt
     1561 gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga
     1621 atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa
     1681 gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca
     1741 gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag
     1801 atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg
     1861 aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga
     1921 ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac
     1981 taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg
     2041 actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga
     2101 aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag
     2161 aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg
     2221 ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa
     2281 tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt
     2341 attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca
     2401 atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata
     2461 ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat
     2521 gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac
     2581 ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga
     2641 gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc
     2701 gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt
     2761 tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct
     2821 tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt
     2881 ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg
     2941 tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc
     3001 aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa
     3061 gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt
     3121 gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc
     3181 ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt
     3241 cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa
     3301 tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat
     3361 ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc
     3421 tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga
     3481 agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat
     3541 cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga
     3601 gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat
     3661 cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa
     3721 tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa
     3781 tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg
     3841 tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt
     3901 tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt
     3961 cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata
     4021 agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta
     4081 ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct
     4141 ggttcaattc
//
LOCUS       DAZUKD010000175         3889 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000175 DAZUKD010000000
VERSION     DAZUKD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.181"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..1304
                     /locus_tag="ACG8N9_002740"
                     /pseudo
     CDS             261..1304
                     /locus_tag="ACG8N9_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(1509..2828)
                     /locus_tag="ACG8N9_002741"
     CDS             complement(1509..2828)
                     /locus_tag="ACG8N9_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFS4898273.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            2986..3270
                     /locus_tag="ACG8N9_002742"
                     /pseudo
     CDS             2986..3270
                     /locus_tag="ACG8N9_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3263..3610
                     /locus_tag="ACG8N9_002743"
     CDS             3263..3610
                     /locus_tag="ACG8N9_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898274.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            3607..3789
                     /locus_tag="ACG8N9_002744"
     CDS             3607..3789
                     /locus_tag="ACG8N9_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898275.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         1342 a          600 c          744 g         1203 t
ORIGIN      
        1 taaaacagca tagttctcaa attgtttaaa atgattgtca agttaaaaaa atctgggatc
       61 atataaaata gtatagttct caaatatgat tagaaaccct attaaagagc aatattaagc
      121 caacttactt aaatcgttta attaagaaca gtaatctctt ttagtcagac ttttagtctg
      181 acttttttgt atttccaaaa tttattcttt acaaaaacga acatatgttt gtataattgc
      241 ttgtgaatgg gggaataaaa atgtttgatt acagtagaga accggtgaat gactattttt
      301 taatcgacat gaagtctttc tatgctagcg tggaatgtat tgaacgaaat ttagacccaa
      361 tgactacacc gttaattgtc atgagtagat cggacaacac tggttcaggt ctgactctag
      421 cttcttcacc tactgcaaag aaaaaatatc atattactaa tgttagtaga gctactgatt
      481 taccttatcc attaccaaaa gaacttcata ttgttccacc acgaatgaac ttatatataa
      541 aaaaaaatat ggaagttaat cagattttta gaaagtatgt cgcagatgaa gatttattga
      601 tttattcgat tgatgaatca attttaaaag tgacaaaatc cttaaattta tttactagtt
      661 caggaacaag aagcgagaga agaagagaat tagctaaaat aatcaaagat gagattcaga
      721 aagaaacagg tctaatagca acagtcggaa taggagataa cccattatta gctaaactcg
      781 cactggacaa tgaagcgaag agtagtaaag aatttatagc tgagtggtct tatgagactg
      841 tacctgaaaa aatatggaaa ataaacgaga tgactgactt ttggggcatt ggatcacgca
      901 tgaaaaaaag attgaatcat atgggaatta tgagtataga agatttagca aattggaatc
      961 cctacacaat taaaagtcag ttaggtgtaa tcggacttca gttatatttc cataccaatg
     1021 gaattgatcg tacggatatt gctattccac cagaaccaac caaagaaaag tcttatggga
     1081 attcacaagt tctaccaaaa gattatacga ggagagaaga agtagaacta gtagtcaagg
     1141 agatggcaga gcaagtagcc attcgaatta gacaacacaa ttgcaaaact ggttgtgtac
     1201 gcttagcaat tggtacgtca attttggaag aaaaatcagg tttctcgcac cagatgaaaa
     1261 taccaattac agataatacg aaagaattac aaaattattg tctttcctga ataattcata
     1321 gctttaattc tttggcacat tttattgaaa tatgtatcac taatatccct atcaactgat
     1381 tttgactaaa aagttacttc aaacattcac tgttcaaaaa tattgaggaa taaattttgg
     1441 gtgtaaggca ttttgagcat acttctccct ggataacttc ccagtaaaaa aatcgttaga
     1501 ttgttggatt agatccattg actggctcgc ctcaggcgag caaagaccct gtagaattca
     1561 ttctgataca catgataact agataatttg agatagactc gtctacccgt ttggactaat
     1621 ttcccagcaa ctttcagaaa cttcaatcga atagaatgaa tggtcattcc cttttggact
     1681 tcttcaaagc caatcgttct taagaagttg actaagttgt aagctatcaa gctgagcatc
     1741 attcggacat gattctccaa aaagcgagga ctgtctgttt tgtcaaagta aaagccagct
     1801 ttcgcttctt taatgaagtt ctccattgtg ccacgtttgg catagataga aaagatggta
     1861 tcaggagaaa cattttctga tagattcgtc acgataaatt catgtcgaaa gagtagttcg
     1921 cccgcttcac gtgttgagcg tatacacacg cgacgacttt gtgaccagga ttgtgcttga
     1981 taagtggtcg agaagtactg aacttctcgt tcttcccact tttgattatc gccataaagt
     2041 actgatttct cagctatttg acctagtcta cgattattct tcagtcgaat aacataatga
     2101 ctcttttttg attcacacga atcatacaca ccaggtgtag cgaacccgct gtctccacga
     2161 accaagatgt cagtgtttgg tagagagtga ttatagtgct ctaataaagg tgtgagaaac
     2221 tcctttacgc cttttgatgt atattgattt cctgaacgta gttcagcttt taagaagtca
     2281 ccagtcaatc cgtcaaaagc taccaatgga tggtaaccat aggtttggta gtgtgtatta
     2341 taattcgttt gttcttggtg accaaatgta tctgaatggg tcgaatctaa atcaatgatt
     2401 aattccgtat cattacggat gaggcgtgct ttatcgataa gttcttggtt caaagcttga
     2461 agttcatgga tattctcctc agatagtcga tctaaaaatc gggaaagtga agattgagaa
     2521 gcgagttctt tcctatctaa aacagcttta aacacaggat cttgtctcag gagattagct
     2581 gcagaatcag ctgagtaacc agcaattaat tgcataatga actgctcaag tatggataag
     2641 ttgtcatgag taaaatacgc tcgttcgtct tcaatgtgaa tgtgttgctt agccagttca
     2701 gaaaaaccaa agatgttcat cagctctttc actaggacga gacccgaatc actcgataat
     2761 tgaccacctg tatgagaaat agtgatattt gaattgaatt ttacttggtt tttgtgtaag
     2821 ctagtcatga aaagaactcc tttcttttgg ttggtttagt cactttaacc atagcagaaa
     2881 ggggttcttt tttcatcact taacgggtga agatgaaaaa gtaattgtaa gctgccgtga
     2941 ggcagtttca catggtttta attaaaagta tgaattattc agggtctttt tttattcaat
     3001 aagttttatg aggggcagga agtacgacat ataggagtaa cttatagcag attagtatac
     3061 acagattccc tacaattaga tttatttagt gatccaagaa aaaagattgc tgaagaaaat
     3121 ttggatagga ttattgataa aattagacaa aaatatggat ttacagctat tatacatgct
     3181 tcatctatgt tagaaggtgc aagaagtatt acgagaagca ctttaatagg aggacacgca
     3241 ggaggattag gaggtttgca taatgattga atataacgat agaggacgtt taaaatggtc
     3301 gggattttat ttgtctgatc atactgaaag aatcaatgca gacaatgtac aacgtgctaa
     3361 tcataatttg gcaaaagaac aaatgacgac tgaagagatt gcgaaaatac tgagaactgc
     3421 tgtcataaaa aatagacccg tctgcattca aaaagaggaa cgaaacgctg aaggttacta
     3481 tccagatgat gtagtaggaa aaattattgg atatgacgag cttggtgtat ttgtggaaga
     3541 agaaaaagtt cactatgatg aaattaggaa tgtttctttc gcggaattat taaaatggag
     3601 tcagatatga acattattaa aaaatacgaa ttcggttata ttacgtatga acagctaata
     3661 gaggaattat ggggatatgg tcaacaatta attaatgaag tggggattga ttgcttttgt
     3721 ttctatattg aatccggagc aggataccat aagaacagat actatatatt tccatatcca
     3781 ccaaaatagt ctcttagaga tttttaaatt ttctccaaat actttcaatt attgtgaaat
     3841 atttcctttt aattaatcaa attttttata aaagtaatat ataaaataa
//
LOCUS       DAZUKD010000176         3878 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000176 DAZUKD010000000
VERSION     DAZUKD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.182"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACG8N9_002745"
     CDS             complement(718..3867)
                     /locus_tag="ACG8N9_002745"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS4898276.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYAQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          804 c          636 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gcgtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAZUKD010000177         3805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000177 DAZUKD010000000
VERSION     DAZUKD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.183"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1368)
                     /locus_tag="ACG8N9_002746"
                     /pseudo
     CDS             complement(1..1368)
                     /locus_tag="ACG8N9_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="ACG8N9_002747"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="ACG8N9_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4898277.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="ACG8N9_002748"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="ACG8N9_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2547..3626
                     /locus_tag="ACG8N9_002749"
     CDS             2547..3626
                     /locus_tag="ACG8N9_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4898278.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         1096 a          819 c          838 g         1052 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt ggcggatttt ataattaact
     2461 tagccatttg gaaataaaaa aacgccacgt gaattccatg ttatattgaa gttaccacaa
     2521 ctctcaatag acaaggatga attcacatga cgcaaaacca tgataacaca gtttcacgca
     2581 agggaaaaca cctaacctac tcagaacgtt gccaaatcgc catcctgaaa aaggaaaact
     2641 attccaaccg tcaggttgcg gatgtcctgg gctgcgtgcc gcagaccata aacaacgaaa
     2701 tcaaacgcgg gaccgttact cagcttaacc gccagaatca gaacgggaag gtctacgact
     2761 attatcatga cgtctacgac gccgatgccg ggcaggcggc ctacgagaag aaccgcttga
     2821 actgcggcag acggccctta tgggccgaca cggacgcctt cattgaatgg gcggacgaca
     2881 tgatgctgtt ggaaaaatgg tccccggacg tggtggtggg ctttgccaga aaacacgaat
     2941 tgttcgaccc ggccattatc cctgggacca ccaccctcta tcagtggatc gaccgtggca
     3001 tcatgaaaac gaagaatatg gacctgctag aaaaactgtc ccgcaaaccg aaatcgtccc
     3061 gtcatggcaa ccgcccgaat aaacgggtgc tagggaaatc cattgaagag cgcccggagg
     3121 cggttggtac ccggaaaacc ttcggccatt gggaagtcga cacggtcgta ggaaacaaga
     3181 ccaagtcgga tgccgtgctg ctgacgttgg tggaacggca gacacgcttc gaggtcattc
     3241 tgaagctggc cgggaaggat gccgaatcgg tcaaccaagc ggtccgacag ctgagagaac
     3301 gggctggtgc gtacttcggc cagctgttca agacggttac ctcggacaac ggctcggagt
     3361 tcgcgggact gcacgatgcc ttgcaggatg tggcaaacat ctacttcagc cacccctatg
     3421 cttcctggga gcgcgggacg agcgaaaacc aacacaagct gattcgccgt ttcatcccca
     3481 agggcagccc aatcggcaac gtgagcgaaa accaaatctt gcggattcag cgttggatga
     3541 acgattaccc gcgaaagatt ttgggctatg caacgccgca tgacacgttc gtccaggcat
     3601 ttaggcagga acggttgacg gcttgacacg tgggcccttg acaacgagcc tccctcggca
     3661 tggcttgtgt ctggaaagct ggccggggcc agccagtcgg gagactacca tgcctcagct
     3721 cattgtcaag ggcccactcg attagaaaaa ttagacggtt ggtggtaacg agcaaccggg
     3781 tcaccatggg tggctaactt aaact
//
LOCUS       DAZUKD010000178         3800 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000178 DAZUKD010000000
VERSION     DAZUKD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.184"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..2151)
                     /gene="tkt"
                     /locus_tag="ACG8N9_002750"
     CDS             complement(154..2151)
                     /gene="tkt"
                     /locus_tag="ACG8N9_002750"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4898279.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2253..2504)
                     /locus_tag="ACG8N9_002751"
     CDS             complement(2253..2504)
                     /locus_tag="ACG8N9_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4898280.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2651..3274
                     /gene="lexA"
                     /locus_tag="ACG8N9_002752"
     CDS             2651..3274
                     /gene="lexA"
                     /locus_tag="ACG8N9_002752"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4898281.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3453..3671
                     /locus_tag="ACG8N9_002753"
     CDS             3453..3671
                     /locus_tag="ACG8N9_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898282.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1049 a          811 c          705 g         1235 t
ORIGIN      
        1 acacaaacta atccgaagaa ttctttgttt tgtcatttaa atgtaacata gccgaaaacg
       61 aaaagtaata ttgatttatt cttctaacac ataaagaaca gctgtctcca acttgcagac
      121 agctgttctt atcagtcaaa caattgcttg gatctatgat agttgattgt acgtattcac
      181 gacattttct actgtaaatc caaattcttc taaaatttta gtgccagggg cagatgcacc
      241 gaaatgatcg attgtaatcg ttgcgccctc tgttcctacg tagcgttccc aaccaaatga
      301 tgcagcagct tcgatagcca cacgtttctt cactgattta ggaagtacac tttctttata
      361 ttcagaagat tgtttttcaa agaggtcaaa gcttggcata gaaacaacag aaacatcttt
      421 gcctttttca gccaattctt tttgagcttt cacagccaaa tcaacttcag aacctgttgc
      481 aatcaaaata ccttctggtg tttctccttg tgatggagaa agaacatacg cgcctttttt
      541 caccatttca tctgctactt cttttgtaga aggcaatact ggaaggtttt gacgactcaa
      601 tacaaggata gtaggcgcat ctgttgtctc catcgctact ttccaagcag cacgtgtttc
      661 atttccgtct gccggacgaa tcacttgaac accaggcatg cagcgaacag atgctagttg
      721 ttcaatcggt tcgtgcgttg gtccgtcttc tccgacagca accgagtcat gtgttaagac
      781 gtaaacaaca ggagtatgtt ggattgctgc taaacgaact gctggtctta gataatcgac
      841 aaagacaaag aatgttccac cgtagatacg agttccgcca tgaagttgga taccattcat
      901 agccgaagcc attgcaaatt cgcggacacc gaaccagatg ttgcgtcctt cataatgttc
      961 aggagtgaaa tctttatctg cagccaccat tgtattattg gaacctgaca agtccgctga
     1021 accaccccag aagcttggta tagctttcga taattcttgg atgacttctt tacttgaaac
     1081 acggcttgct tgactgcttc caacttcata agttggcaat tctgcatccc agttttctgg
     1141 tagtttgcca tcaaaagcat cttcaaattg ttgtgctaat tcaggataag cttttttata
     1201 attggcaaac atttcattcc atgcatcttc agctttttgt ccttcttcta tcatcgtttg
     1261 atggaagcgg gcagctactt cttctggaac agtgaaatct ggatactccc aaccataaac
     1321 ttctttagcc atcttgatgc catcttcacc aataggagca ccatgaacag cagatgttcc
     1381 ttctttagga gcaccatagc cgatgactgt ttttacttcg atcaatgttg gtttgtctgt
     1441 ttcagctttt gcttcttcaa tcgctttaga gattgcttct aggtcatttc cgtctttcac
     1501 taagatatgt tgccagccat atgcttcata gcgagcacca acgttttctg tgaaagcttt
     1561 tgaagttgga ccgtctagtg aaatatcgtt tgaatcgtat aaaacgatca atttccctag
     1621 tttcatatga cctgccatcg aacttgcttc ttgagatacc ccttccatca agtctccgtc
     1681 accgcatatt gcatacgtat agtgatccat cacattaaag ttttctttgt tatacgttgc
     1741 tgcaaggtgc gcttctgcca ttgccatacc gacagccatt gcaatccctt gtcctaatgg
     1801 ccctgtagtg gcttctacac catctgtgtg atgtacttca ggatgtcctg gtgttttaga
     1861 atcccattga cggaattgtt tcaaatcatc gattgtcact tgatagccag ctaaatgaag
     1921 caagctatac aacattgctg acccatgtcc tgccgaaaga acgaaacggt cgcggtcagc
     1981 ccagtttttt gatgtcgttg gatttacttt caggtgtttt gtccataacg catatgccat
     2041 aggtgctgcg cccatcggca atccaggatg tcctgagttt gctttttgga ctgcttcaat
     2101 acttaatgta cgaattgtgt taacgccaag ttggtcaatt ttgtcaaaca aaataacccc
     2161 tcctattaat ttaggttttt tttaccttta tttcttacac tttcattcta taagatgtca
     2221 cacgaaaaag caatcttttt cagtattttt tactaaaagt tgtcatttcg gttgtgtaag
     2281 ccttttttct tttggatttc ttttaatttt tccggggtca cgtcattgcc ctcaggatcg
     2341 acgactttca ttccttcgat atgatgacgc ataccggaac gaaaagcttc caaatactct
     2401 ttgcgtaagt tgatttgttc ttgttcttcg gcttttgaga gaccattttc cttcttttta
     2461 cgtgcaagtt cattgatcct ctctattttt tcaggtgaca gcatgctcag ccctcctttt
     2521 actctccttc atattatata aaaactgaaa aaaaaacaac tagataaact aaataaaata
     2581 cgaacattcg tttgatttag cgctttttat atggtactat ttatttatca agtgaaagaa
     2641 ggtgtacctt gtggcccgac aaactgaatc aagacaaatc gaagtcttac gatatatcca
     2701 tgaacaagta acccaaaaag gatacccgcc gactgtccgt gaaatcgggg aagctgttca
     2761 gctttcttct acttctactg ttcacggtca tcttgcacgg cttgaaaaaa aagggtttat
     2821 ccaaagagat ccgacgaaac ctcgtgcaat cgagttgaca aaacaaggtt tagaaaagat
     2881 tgggattcgt ccaacaacta ttccagtact tggcgtcgta acagctggag aacccatttt
     2941 agcagtagag gatgcttcag acttctttcc aatccctcct aatctttctt ccgaagaagg
     3001 cagtctcttt atgctgacaa ttcgtggaga tagtatgatc aatgctggga tcttggatgg
     3061 tgacaacgtg atcgttcgga agcaagaaac tgctcaaaat ggcgatattg tcattgcaat
     3121 gactgccgag gacgaagcga cttgcaaacg attctataaa gaaaaaaact ttttccgtct
     3181 gcagcctgaa aacgacttgc tcgaaccaat catcttagat caagtatcta tattaggacg
     3241 agtagtagga ctttatcgta gtcatatcta ttaattgcta ctaataaaag aaaacttttt
     3301 aaaaagagtt tgagacgatg gtctcaaact cttttttcgc ccgggttctt cttctttcaa
     3361 atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa tatacgaaca
     3421 attgttcgca taaattaacg aggtgagaat tcatgaaatc aatgagacgt cttggcttag
     3481 tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt ccagctttac
     3541 ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc
     3601 aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac tacagatcca
     3661 ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta aacttttacg
     3721 ggggtgtgac aaaaatcggt cacatccttt tttcgaataa acggtgttct aaagccagtt
     3781 tttcggtatt aagtcaaatt
//
LOCUS       DAZUKD010000179         3783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000179 DAZUKD010000000
VERSION     DAZUKD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.185"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            466..2310
                     /locus_tag="ACG8N9_002754"
     CDS             466..2310
                     /locus_tag="ACG8N9_002754"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4898283.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2366..>3783
                     /locus_tag="ACG8N9_002755"
     CDS             2366..>3783
                     /locus_tag="ACG8N9_002755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4898284.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSF"
BASE COUNT         1222 a          615 c          804 g         1142 t
ORIGIN      
        1 acaattatga atgccttttc tttatatcaa ataaagttta tataaaaaac ctcatcctgc
       61 aagaaagaaa aaatgaaccc attcttttta tactaggtct taaaaagaca gaacatctgc
      121 tagaagatag aagagaataa gagttatata tgttggaaga tattcttaga aaaaagacaa
      181 aacttttaga acacgaaaac tatattgaac gaataaaatg aaaaaatgat taaaaaatca
      241 ttttattatt tacgtttaca ttctaatatg ttaaattgaa tataacttaa acaggcatgt
      301 gactggtaat gcaggcagga cctaaataag ttgaataaca atgtatgcgt ttcaatacac
      361 gtttgccttg ttgtcaattt atttagtttc tgcctgtttt ttgtgttgag gatggccact
      421 tcgactcaaa agttcatcac tcaaaaaaga aaagagggaa acaccatgaa ttacaaacaa
      481 acagcagaag aagtattgca gtacatcggt ggggaagaaa acatatccca tttggagcat
      541 tgctcaactc gtttacgttt tacgttaaaa gacaacagca aggtaaatat gagccagtta
      601 gaaaaagtgg atggcgttat gggcgttcgc caaaatgtgc aatgccaagt aatcattgga
      661 aacgatgttg ttgaagtgta tgatgaagtg aaagcgttat taggtgacaa tgtttcaaat
      721 actgaagaag caccaaaaca aaaacaaaaa ttaggttcag ttctattaga ttttattatt
      781 tctattttcc aacctttaat tcctgcgatt gcaggtggag gagtattgaa gtccttgttg
      841 ctactagctt ctatcttagg gctgattagt gaccaatctc aaacctatca gattttaaat
      901 ttggtcggcg gggcaccgct ttatttcttg cctattctgg tggcaatgac tacggctaat
      961 aaattgaaag tcaatcaatt ggttgcagta tctgctgtcg gtgcattgat ccttcctgaa
     1021 atgacagcta tgttggcgga aggtgctaca ttcttatctt ttggattgga taatatagcg
     1081 tatgcatctc aagtattccc agccatctta actgttttat tttatgctca acttgaaaaa
     1141 tggtttacaa aagtttcacc aaaaccaatt cgtattttct ttgtacctat gatgagctta
     1201 ttgattacag ttcctattgc tttgttgctt ctaggaccat taggttataa tgtaggaaca
     1261 gctttctctt ctatcattgt gtctttgtac aatcaatttg gatgggttgc aaccggagtc
     1321 ttagcagctg tactgccatt aatggttgta acgggtatgc ataaagcaat gatcccttac
     1381 gctgtttcat ccatgagtga attgggtgca gaacttcttt atctcccggc ttcattggca
     1441 cacaatattt cagaagctgg cgcatgcttt gctgtcagta tcaaaacaaa agacaaaaaa
     1501 ttgaaatcta cggctgtttc tgcgggtata tctgcattat tcggaattac tgaaccagct
     1561 ctttacgggg taacgattct gcacaaacgt gttttatatg ccgttatgtt ttctagcctg
     1621 atcgggggag catttgcagg aatcgttgca cttcaagcct ttgctttagt gggtcctggg
     1681 attgcaagta tcacgatgtt cgctgacaaa gctgacccaa tgaatctggt ttgggcattt
     1741 gcagtatcgg ggatttcatt tgtggttgct tttgctgcag cgttggttct ttataaagat
     1801 aaagaagttg tcaatgaagt tgaaactaag atggaaacgg ccgttgtttc agatgctgtt
     1861 ctttcaagtc ccgtgaatgg aaaagtaatc tcactatcag aagtaaaaga cactgttttt
     1921 tctagtggta tggtaggtga tggagtagca atcattcctg aagaagggat attagtagct
     1981 cctgaagatg gagaaatcac aatggtattt gaaacgaagc atgctattgg tttaaaaacg
     2041 gctaatggtg cagaacttct ttttcatatt ggtattgata ctgttcagtt agaaggaaaa
     2101 gggtttgagg catttgttcg ttctggtgaa aaagtagcca aaggacaaaa gctgattgca
     2161 tttgatattg atttaatcaa agaagcagga ttcgacccca cagttatctg tgtggtaacc
     2221 aataaggata attacactat ccgttcattt aacctagaaa aattggtaac caatgatagt
     2281 gatctcttag ctatcagtcc gatagcttaa acacaagata gtgaaaaaaa atatattgaa
     2341 tgaaggaata gaggagaata gaataatgac aaacacaaca agttttccaa aaggattttt
     2401 atggggcggc gcgatagcag caaatcaagt agaaggtgct tggaatgtag acggtaaagg
     2461 tctttcagta gcggatatgg ctacttataa accaaattta gacaacaaag attttgctgc
     2521 tcatatgcat gtcaccctgc agtctataaa agaagctagt gaagacaaat ctgataaatg
     2581 gtacccaaaa agaaggggcg ttgattttta tcatcactat aaagaagatt tagctttatt
     2641 tgccgaaatg ggctttaaaa ccttgcgcct ctctattgct tggactcgct tgttccctac
     2701 aggagaagag ctggaaccga atgaaaaagg ggttgcattt tatgaaaacg tctttaaaga
     2761 aatgcaacgt ttaggaattg agccgattgt gacactatct cattatgaaa tgccattagc
     2821 tttagcagtt aaatataatg gctgggtgga gcgtcgagta attgatgatt ttgttcgctt
     2881 cgctaacgtt tgttttgatc gcttcggcaa atacgtaaaa tattggctga catttaatga
     2941 gatagacagc atccaccgtc atccatttac aacagctgga attatccaag agaaaagcgc
     3001 agaaggtcaa gaagctcaag atatctatca agcattgcat catcaatttg ttgcgagtgc
     3061 gttagtgaca aaagatgctc atgaaaaaat tccaggaagc caagttggtt gtatgttaac
     3121 gaaattgatg acttatccat taacttgcgc accagaagac gttgagctga ccttgaaaaa
     3181 gaacttagaa aataattttt atgcagatgt tcaagttaag ggagaatatc ctgctctaat
     3241 cgaaaaggat ttggaaaatc ggggaattca gattaaaatg gaagatgaag atcgagaaat
     3301 tttaaagaaa cataccgtag attttgtttc tttcagctat tacatgtcca tggcagaatc
     3361 agctgatcca aacgcagaac gcacaccggg aaatactgtt ttaggggtta agaatcctca
     3421 tttgccttca acagattggg gctggcaaat cgatccaaaa ggtttgaaaa tttcgttgat
     3481 cgaactttat gatcgttacc aagtaccgtt gatgattgtg gaaaatggta tgggagcagt
     3541 cgatcaggta gtagatggta aaatccatga tccgtaccgc atcgactact ttaaagcaca
     3601 cttccaacaa atgaaagaag caatcgatga aggtgtagat ttgatagctt atactagctg
     3661 gggaccaatt gatttagtta gtgcaggaac ttctcaaatg tctaaacgat atggatttat
     3721 ttatgtggat gcagacgatg aaggaaatgg gacatataac cgcttgaaaa aggattcgtt
     3781 caa
//
LOCUS       DAZUKD010000180         3771 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000180 DAZUKD010000000
VERSION     DAZUKD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.186"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..382)
                     /locus_tag="ACG8N9_002756"
     CDS             complement(47..382)
                     /locus_tag="ACG8N9_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898285.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(449..1819)
                     /locus_tag="ACG8N9_002757"
     CDS             complement(449..1819)
                     /locus_tag="ACG8N9_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4898286.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(1897..2433)
                     /locus_tag="ACG8N9_002758"
     CDS             complement(1897..2433)
                     /locus_tag="ACG8N9_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4898287.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            2742..>3771
                     /locus_tag="ACG8N9_002759"
     CDS             2742..>3771
                     /locus_tag="ACG8N9_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898288.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSS"
BASE COUNT         1181 a          768 c          543 g         1279 t
ORIGIN      
        1 cacaacccct tatttcgaat gaatagtgtt caaaagctag tttctcttac atcatttttt
       61 caacaaaatg cattagccgg tcttttacat cttcggttga atctttgatt gaatcaccat
      121 aaacagagat tttctttttt cctgatttga tttttgctaa cattcccatc aaagaatcca
      181 agtcactatc tgaaagacca tctacgattt ctagtaattt ttcatttcca tcaaatttgt
      241 catagacgaa tttttttact ttcgtccgag tagtcatatg atggatcttt tccattactt
      301 ttcccgatac aacgactgct gtcgctacac tggcagcggc tgctacgctt aatccgattg
      361 ttacttttgt tgatgttttc ataagtatca gctccttttt atctatctcc atcatagcat
      421 agacaaaaaa ataaacgaac taatccactc agtgaaatct caatccttta gggaaaaagt
      481 tctttactgt tccttttact tgtttcccaa atccgacagg tatcttatct aatactagca
      541 aaacccatcc ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt
      601 ccttttgagt gatcggcaag caaggtatat tctctatctt ttttgtagca agcgctaatg
      661 catagctagg ttcaaatcga ttctttttga aatctcctaa atgcaaacct gtacgtacga
      721 cttttaaacc atcgagagaa ggggctaact caggaacttc ccaaagatgg tcgttgaaca
      781 ctaaaagtct tccagtagct tcataatgaa aatcatttga aaactccgtc cagagttttt
      841 cctgctcttt tgtcatttgc actttacttt tttttcgtgt cttcttttct ttatgcatct
      901 gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga tctttatgag
      961 gccatatacg tattgttttt tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac
     1021 taacggattg agtcaaagga atctcctcaa tcgttactgg ataattttct acaagccagc
     1081 taatgatctc ttcattttct tctggtgcaa acgtacaagt cgaataaatc agttgtcctt
     1141 tattttttag catttttatt gctgaagaca aaatctcttg ctgacgtttt tgacagtata
     1201 gcggagattc ttctgtccac tcttttattg catttggatc ttttcggaac attccttctc
     1261 ctgaacaagg agcatcaacc acgatccgat caaaaaagcc cgaaaaatga gggacaagtt
     1321 ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc ccatcgttct atattttctg
     1381 ataaaatctt cgcacgtttc ggaaagatct cattcgtcac taataaccct ttccccttca
     1441 tttgagcagc taattgggtt gatttaccac cgggagccgc acacaaatcc aaaacttttt
     1501 caccgggttt tgctgcagca gctgttccaa cgatcattgc acttggttct tggctatatt
     1561 catacccagc ttgatggagg aaagactttc cattgacagt gccaagaaat ccctcattgc
     1621 tgtagggagc gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg
     1681 gttttagtgg attccaccga aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa
     1741 aatcggatgc ttcttcgcct aaaagaagac gatatttttt gataaattgc tgtggtaacg
     1801 ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt ttacaatctt ttttacgaaa
     1861 aataaaggtg gctgatctac tttttcatgt tgctgcctaa ggtgactgtt cctttaagat
     1921 attttccaag tattcatccg tcacctcgtt tttatcccaa acagcgttca tcatttcaag
     1981 aaaatcttga gaagaatcat ctgagcgcaa acttcccaat gcattaagcc ataccaattc
     2041 aagcgacttt tcttctttga tcttcgtgtg acctttgaca tatgagaaaa atggccgctt
     2101 aaatagcgaa tccatgtcta aatcagattt tgcaatgatt tcacgaatac tttcatggat
     2161 agaaaaaaaa tctttcaata agtagacaat gcttaaaaca tcatcggaac tgcattcagc
     2221 taagtagttt tcaaaataaa cagtgatttc ttctaatatc ttctctttta acccgctgat
     2281 tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg cagaactctg ctaactctct
     2341 tgcagtaatt gacttcattc catattcttc cacgtatacc atacttttga acagcaaata
     2401 ttctttacta taatttgtta ctctccccat cattcttacc tccgtctctt ttcatctacc
     2461 cataaataaa taacataaat aattctaaaa atattattga taattatcaa aacatattta
     2521 gttatatcca ttaaaaatca atttttccgc cttaaaaaca agaatatctt gacaaaatac
     2581 cctaggaaat aaacaataga ataaacaagg taacaaaaaa attaaataac gttatataaa
     2641 aaagaaggcg acactctgtg agaataacac agactgaaat gactttccac tcacagtttt
     2701 tgtctaacta tatacaaaaa cagaataaaa aggaggtatt tatgctaatt gaagcaagaa
     2761 aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa taattgttct ttacaagaat
     2821 tatccaatga cttacatatt ccaaaacgga caatcaaaga actgatccga aaaataaatg
     2881 tatcactcaa gcaattttta ttgattgatg aatttattta ttctaatcat aaaggagaga
     2941 tccaaaccaa agaaaccgat caaactaaaa agttagctgt tttttctaag ttaaaattat
     3001 gctacctaaa agaatccaat cgttttaact atttgatgtt actaatcaat tatccacaaa
     3061 ctgctgtacc aaaaaaatat ttacttgaac aactttatat atcgccctcc tatctcgaaa
     3121 aactgacaaa acagctcaat cattccttaa aaaaattcca tttacggatc ctatctgtaa
     3181 aaaattgctg tactcttgaa ggaaatgagt tatctatccg tctctatctt tacttttttc
     3241 tttcagatac atttggaggt aacgattggc cattttcctc acttgaatta aatgcactaa
     3301 aaatgaacag tacagatgag caaatagaaa aaatttcaaa acaagaaaaa aatatctact
     3361 tgctgttaac tttattcata ttacgaaaca aacaacaggc atattttcca aaaacttctt
     3421 ctgaaatcac tgaattgatg gacttattgc agcatacaca tgattattca gaatacttta
     3481 cttctttttc agtgagtcgc ccttgtgaag agacagaaac agagagagcc tactttaatt
     3541 attttttcta ttgttttttt ccaaatcaaa tctcttttcg acaaaaacag gagattggtt
     3601 ttttattttc cacaagcaaa aaccttattt gtcgacaagt cggtcaattg atgacttact
     3661 taactcaaca gtttcctctc aaccatttag aaaataaaat accaatttat ttttattttc
     3721 ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc cagcagtagt t
//
LOCUS       DAZUKD010000181         3588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000181 DAZUKD010000000
VERSION     DAZUKD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.187"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8N9_002760"
     CDS             127..579
                     /locus_tag="ACG8N9_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898289.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8N9_002761"
     CDS             593..1282
                     /locus_tag="ACG8N9_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898290.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3588
                     /locus_tag="ACG8N9_002762"
     CDS             1407..>3588
                     /locus_tag="ACG8N9_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4898291.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDET"
BASE COUNT         1214 a          479 c          745 g         1150 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacag
//
LOCUS       DAZUKD010000182         3568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000182 DAZUKD010000000
VERSION     DAZUKD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.188"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8N9_002763"
     CDS             <1..435
                     /locus_tag="ACG8N9_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4898292.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8N9_002764"
     tRNA            complement(759..842)
                     /locus_tag="ACG8N9_002764"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8N9_002765"
     CDS             1370..1870
                     /locus_tag="ACG8N9_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898293.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8N9_002766"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8N9_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4898294.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8N9_002767"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8N9_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4898295.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT         1119 a          693 c          585 g         1171 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaa
//
LOCUS       DAZUKD010000183         3520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000183 DAZUKD010000000
VERSION     DAZUKD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.189"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..322)
                     /locus_tag="ACG8N9_002768"
     CDS             complement(56..322)
                     /locus_tag="ACG8N9_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898296.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(333..524)
                     /locus_tag="ACG8N9_002769"
     CDS             complement(333..524)
                     /locus_tag="ACG8N9_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898297.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            815..1237
                     /locus_tag="ACG8N9_002770"
     CDS             815..1237
                     /locus_tag="ACG8N9_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4898298.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            1254..1676
                     /locus_tag="ACG8N9_002771"
     CDS             1254..1676
                     /locus_tag="ACG8N9_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4898299.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            1705..1905
                     /locus_tag="ACG8N9_002772"
     CDS             1705..1905
                     /locus_tag="ACG8N9_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898300.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            1992..2441
                     /locus_tag="ACG8N9_002773"
     CDS             1992..2441
                     /locus_tag="ACG8N9_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFS4898301.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            2749..2880
                     /locus_tag="ACG8N9_002774"
     CDS             2749..2880
                     /locus_tag="ACG8N9_002774"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4898302.1"
                     /translation="MQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRVK"
     gene            3018..>3520
                     /locus_tag="ACG8N9_002775"
     CDS             3018..>3520
                     /locus_tag="ACG8N9_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFS4898303.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMK"
BASE COUNT         1321 a          598 c          594 g         1007 t
ORIGIN      
        1 attagtggcg cgctgaatac taatgttcga ttaaactttc ccatgttgac ctcctttaaa
       61 ttttgtattt agccatcagt tcatcgatgt cctttttgtc atatcttaaa aagccatcta
      121 tacgaatttc ttttaaaccg tgagctatta acttctcaaa accttgatcg tttacacctc
      181 ccacatattt tctagcttcg atctttttta aatatcgtgt ttctgacaaa ttcttttttg
      241 aaagttgact aagtgcgtcc tttacaactt caacaattgc aattcttaaa cgagtcaaaa
      301 actcttcgct tagaatattc atgacttgcc tcctatcgaa ttctgtaatc acggatgatc
      361 tctagaatag ttctgtttgc ctttggtcca ctccagtgtc catcaagaat ttgttgcatt
      421 cgtacacgcg tgtgaccata agcagtagcc aaatcatcca tagttattcc attctctttc
      481 ataaactctt tgatcgccgc acgaccgtta tccaagtttg acattaaccc acttcctttt
      541 tttataaatt tgtaagttaa aatgatagaa aaaatataat ttccattgac atttttacac
      601 tatagagtaa aatgaataca tagagaaata agcctataac aaaacctttt atttgcactt
      661 ggtcgccaaa ctttatgcta taaggtgtgt ttttagtttg cttttttcta tcaaattaac
      721 ttacggataa ataataactc tatatcgtaa aattgtaaag ctatttttac tctttttcgt
      781 aaattatttt ttcgtgttgg aaaggaacgt tggaatgaca acatttgaaa gaataaaaga
      841 actagcgaaa aacaagaata aaaaccttca acaagtcgct aaagatttga agtacggaga
      901 gaattacttt tatagtttaa acgcaggaaa acagccaact gccgaaaagt tacaagaaat
      961 tgctgactac ttcgacgtat ctgtagatta tttgctagga cgtactgaga aaaaaagata
     1021 ctatgattta acagaaaaag acgagcgtga tatccaaaaa gaattgcagt cattaatcga
     1081 cgatttatct aatgctgatg gaatggcttt ttcgaagaaa gataatgaaa tgagtgaagc
     1141 gacacgtgaa gctctcatta tttctttaga gaatgctttg cgaatagcta aaatagaagc
     1201 taagaaaaaa tacacaccta aaaaatatcg cgattaaaag gagtacgttg tttatgtatt
     1261 tacctcaaat tgataacaaa attaataagc tggtaaaact atatcaaaca cgcaatcctt
     1321 ttagaattgc acgagaattg aagatactag ttttagaaga agatttggga gaaatttatg
     1381 gttactatag taaattcaaa cagataaaaa tgatccacat aaattctagt ttagatgaga
     1441 tgggaaaatg gataacttgt agtcatgaat taggccactg tgttttgcat ccgaatgaaa
     1501 acacacctat gttgtctaaa aaaacgctag tatctgaatt aaaaattgaa aaagaagcaa
     1561 attatttcgc aactcatctg actattgatc caactttaga cggctttgat catatgacaa
     1621 aatatgaaaa gctcgtctgc tttggattac cagatgaatt cgatcgtttc ttgtgaatta
     1681 taatacaacg taaggaggat atagatgaaa ataggaatga gaaaaccaag tataaaaaaa
     1741 tccataagcg ctagaaccac tggaaaagca aaaagaaaag taaaaaaagc agttattcct
     1801 ggttatggga aaaaaggtag cggctggatt aaagacccaa aaaaggctgc ttacaataaa
     1861 gtatacaaga aaactacatt cagtttttgg gatttattta aataaaaaaa gcccgtgcag
     1921 caacacggac tcgaaacctt atttctaaga ttccccaata aaaatcatat catagaaatg
     1981 aggaataaaa aatgaaaaaa gtaataacaa gtttattagt ttttgcttct ctgttacttt
     2041 tcactgcttg tggtaaaaaa gtaactacag aagatttaaa agctaatgat tggttgattg
     2101 aagcaaccaa agatgacgaa cctaatatga tcgtatcatt ttctgatcac gtcgtatctt
     2161 ttagtgttga cacaagttct atgaaatcaa cttcaagtaa cgattgggag gctatgggag
     2221 aagaatttgc taaaagctta gtcgaccaaa tgaattacaa atttgagtat acacttgaag
     2281 gcgatgttat gacttggaaa gatgataaag acaaagataa tgatgccaaa tatactgtca
     2341 gcaaagaaga taaaaatatt attttaactc cagaaaagcc taacaaatct gatgataatg
     2401 agaaattagt tttgaaaccc tacaaaaagg aaaaaaacta aagaatctag aacaactact
     2461 agctcaacaa gtagcaacga acaatcatct tcgactacaa cagaaagtac tattgcatct
     2521 tctagtactg tagaacaagc tgctattgcg cagaatacgc ctcctgaaac tagtagtact
     2581 tactctgctg aaagtgtaga aaactatgca gaagctactc ctagttcaag tgttgaacaa
     2641 gtttcaccca ctcccagttc aacggagcaa tcagtaccac aagattatgt accagctaat
     2701 caagctcaac caacggcacc ttcttcatct gaaaccgaaa ccttgacttt gcaacctggt
     2761 gaagggacca aacaaatagc tgaacgagct ggaattacgg tagatcaact gtttgcatta
     2821 aacggtatgg atccaaacaa ctacatgctt tatccaggac aagaactaag agtgaaataa
     2881 aaaaagcaca tttgccgccc gaccaagaac gcaaatgtgc aaagagctac ggtaaagtag
     2941 gtctatttgt tgtaccctat tttaccatct cgaaatccgc tgtacaaccg aacaaacgta
     3001 cgaaaggaaa gataaagatg gcaaaaaaag aaattgataa acgaattaaa aagcacttaa
     3061 caaaaaaagg cgaagaaaaa tatcagttta gtctgtactt aggagttgat cctctaacag
     3121 gaaaaaaaag aaaaacaacg agacgaggtt ttagtacgcc attagccgct gagagagctt
     3181 taaaaagatt ggaggcagat atacaggaaa aaggactgca agcttctaca gctcaaaaga
     3241 gcaagaaatt cgaatatgtt tacggcttat ggtttgaaaa ttataaaaag acagttaaag
     3301 aaagcacttg gtcctccacc aaacaaattt ttgacacaca catcttaaaa gtgtttggag
     3361 ataagttcat tgacaaaata gacgtttttt tctgtcaaga agcagtaaac acttggtccg
     3421 atagccatcc taaaattttt aagaaaataa aaaattacac atctaatgtt tttgattacg
     3481 ctgcttcttt acaaattata actagcaatc ctatgaaatt
//
LOCUS       DAZUKD010000184         3436 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000184 DAZUKD010000000
VERSION     DAZUKD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.190"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8N9_002776"
     CDS             complement(<1..182)
                     /locus_tag="ACG8N9_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4898304.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8N9_002777"
     CDS             517..951
                     /locus_tag="ACG8N9_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4898305.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8N9_002778"
     CDS             974..1294
                     /locus_tag="ACG8N9_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898306.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8N9_002779"
     CDS             1435..1626
                     /locus_tag="ACG8N9_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898307.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8N9_002780"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8N9_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4898308.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8N9_002781"
     CDS             2112..2375
                     /locus_tag="ACG8N9_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898309.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8N9_002782"
     CDS             2399..2587
                     /locus_tag="ACG8N9_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898310.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8N9_002783"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8N9_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898311.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..>3436
                     /locus_tag="ACG8N9_002784"
     CDS             3070..>3436
                     /locus_tag="ACG8N9_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4898312.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAII"
BASE COUNT         1285 a          492 c          692 g          967 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattg
//
LOCUS       DAZUKD010000185         3353 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000185 DAZUKD010000000
VERSION     DAZUKD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.191"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..129)
                     /locus_tag="ACG8N9_002785"
     tRNA            complement(57..129)
                     /locus_tag="ACG8N9_002785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Gln,seq:ctg)
     gene            complement(175..603)
                     /locus_tag="ACG8N9_002786"
     CDS             complement(175..603)
                     /locus_tag="ACG8N9_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898313.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(663..1421)
                     /locus_tag="ACG8N9_002787"
     CDS             complement(663..1421)
                     /locus_tag="ACG8N9_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4898314.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1716..1913
                     /locus_tag="ACG8N9_002788"
     CDS             1716..1913
                     /locus_tag="ACG8N9_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898315.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2019..2501
                     /locus_tag="ACG8N9_002789"
     CDS             2019..2501
                     /locus_tag="ACG8N9_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898316.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2559..3155)
                     /locus_tag="ACG8N9_002790"
     CDS             complement(2559..3155)
                     /locus_tag="ACG8N9_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4898317.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1005 a          555 c          652 g         1141 t
ORIGIN      
        1 cctgcaaaaa gattgttctt ttacaggggg ttcatttttt cagcaagcac atcaaattgg
       61 gatggcaggg ttcgaaccta caattgcttg attcagagtc aagtgcctta ccagtttggc
      121 tacatcccat ttattcaaca aaataatcat agcactttcc taaaaaaacg caagttattt
      181 ttcttgatcg gatagttgtt gctttactcg taaaaggcgg cgtactagtt tcttttctaa
      241 tccagcagga agggcaatca gttctgctac tgacaagacg gtgtccaata actgatattc
      301 tttgacaggt tcattcataa tatgatagat gtttgctaaa cgttcaatat attcaaagcg
      361 agtatactct gttcgagcaa ttagaaattt caataaatcg gtggctttct cgtactcttg
      421 atctgcgtaa taaacaagag attgacgata caatttgatt tccagttcat tgagggtagc
      481 aggtctgcga cgctcgttcc actcattaat agagacttta ccttgaacat ttcccttgca
      541 gtcacggaca aaaataatcg gaatttcatt tggatccatt tttttatgtg tgaatttttc
      601 cattatctac gctcccttca aaggtttggt atcttgagta taagggattt ttgtttgaat
      661 tactagcttt tctccaaagt tagcatctgt ccacccattg cagtgaaaat cgccgaaaaa
      721 tcgttgatcg gtactgctcg attttttaca ccatagggat cgttgacata aatattttct
      781 ttgtcatatc cagtgactac acaagcatga acaagaggag tgactcttac ttctccatta
      841 tttgtggtcc aagtcataaa atcagtttca tctgggactt caaaattgat agtcacagaa
      901 gtccagacag gatttccttt agatagtgct tttttgatat catcgagttg agcatcggtt
      961 tttggaaaag tgtgataatc cgtttggacg atttgctgag caagggaggc aactggggga
     1021 acatagacga ccatagccca gtctccgcca ctgatatcac caacaaatcc ttcattagga
     1081 tcgccatgag tcccatctgc attgaaaaca gggacgtaat ccaattgctc agctaactgg
     1141 tttttagttg tttcataacc ataaaaagat aaaagcatag aaagcgaagt gacttcacag
     1201 ccattttcta aagaaggaga atcaaactgg ctctctaatg gaacatctag taaaacttct
     1261 gctttctctt ttgtcttttt cgttttttgt gttgaacttt tttgtgcagt gttcattgta
     1321 tgttctggag aatggatatg taggtaataa cttgtaccgc ttaataaaag agcagctgtg
     1381 ccccctagaa acaatagttt tttgaacgtt tttttcttca tttgaaatct cccttttcag
     1441 ctgtttatct ttcctattat aaatgtattt ttattggaaa tggaagtatt atattttaat
     1501 atgtaggaaa taattagttt ttaaggaata tgttttaatt ttttgaaaaa aattcttcta
     1561 aatacaacag ctgttttaat tgtgaaaacg aaaacagtac gctataatgc ctgtaaggtt
     1621 gatttttaac tgtaaataaa aatcaatgag ttaaatctcc aaagaaatag cagtggtaat
     1681 gtggagaaaa gttccagcag tttaggagga ttttgatgac ggaagaaatg attaatttag
     1741 gagaacaata tgcatgcaaa ccaattggtt ttacaaaaac ggtcattggt gaagttgtat
     1801 caaaaatgac aaactgcgcc gtggtcaaag ttgcccaatg tgctgcagag gaccaagaat
     1861 tattagatga aaaagcaagc atggttgttg caaaatatga tacatttgaa taaagaaaac
     1921 aattggacgt gcggcgtgct gcaagtccaa tttctttgag aaggtaaatc agtattcttt
     1981 tcctattttc tggagaagaa aaagcgaggc gagttgtcat ggtaacattc tttacggttc
     2041 tattgttttt attcgttgtt gtgatgtttt tatttctttg gctggttcga aaagaaatta
     2101 tttatcggac tgtgcgaaat cgatgggtct acttggtgat acccttctta gtgtcactag
     2161 tcatttggta tacactcatc agtcatccta cagcagatga attggcaaaa gggatcttgt
     2221 cagcaatgat ttttattagt tttttgttgg acagtagagg aatcacagaa gaaggacttg
     2281 ttttgaatag ctttgacaaa aaaggtgttc ctttttctga gatcaataaa attgtattat
     2341 atcagccaaa aggttctaaa atcgtaaaaa tgaatttttt ccgaaacgga tggcgaggac
     2401 cgatgctgaa attttctgca tcattagaag aattagttcc atttttgtcc caacgcttaa
     2461 acgatgaggc agagattgat attatgattg atccagaata aaaacagagg ttgcgacaag
     2521 atgtttgtca caacctctgt tttttatttc aaataaaatt atccaaatac gcggaagcga
     2581 tcggcatcgt tcatgtattc tttttcccca gccggtgttt ctggtgaacc gaatactaat
     2641 tgcgaacgta gtttccagtt tgaagggata ttccattctt ttgcaactgc ttcgtcaata
     2701 actgggttgt aatgttgcaa gtttgcgccc aatcctttat caactaatgc tacccaagta
     2761 ttagctgttg cgattccgtt tgattgttct gaccaatctg ggaagttgtc tgcatataat
     2821 tcaaattgtt cttgtagacc ttttaccacg tcagtatctt tgaagaataa tactgtacca
     2881 taaccatttt tgaaaccagc taatttgttt tgtgtatttg ggaaagcttc tgctggagtc
     2941 aatgggcgaa gtgcttcttc agtgatttcc caaagttttt catgtgcatc tccgaaaagg
     3001 ataactgcgc gtgttgattg agcattgaaa gcagttgggc tttctttaat tgcttctttg
     3061 atcaaagttg ttaattcttc attagaaagt gaaacattac gtcctagatg atagattgaa
     3121 cggcggtttt tcaaagtgtc agtaaaagtt gtcataataa aatttctcct ttgatttgcg
     3181 ttttgcattt ttttaattga ttacgaaatg aagtatagca ccttacaaaa agtaagtaaa
     3241 cagatatgct cacaaaaaat aagcgaagtt caaaaaacaa ttttaataag aaaaatcgtt
     3301 atgtaaggat tttcttagtt atcgctttca ttgttgtgtt ttaggaattt ttg
//
LOCUS       DAZUKD010000186         3332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000186 DAZUKD010000000
VERSION     DAZUKD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.192"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..634)
                     /locus_tag="ACG8N9_002791"
     CDS             complement(137..634)
                     /locus_tag="ACG8N9_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4898318.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(764..1474)
                     /gene="budA"
                     /locus_tag="ACG8N9_002792"
     CDS             complement(764..1474)
                     /gene="budA"
                     /locus_tag="ACG8N9_002792"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4898319.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1487..3151)
                     /gene="alsS"
                     /locus_tag="ACG8N9_002793"
     CDS             complement(1487..3151)
                     /gene="alsS"
                     /locus_tag="ACG8N9_002793"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4898320.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          941 a          725 c          539 g         1127 t
ORIGIN      
        1 ttaaaatatg attatgaaaa tcaataacac ttgtgatttt ataaaaaaat aaaaaaaccg
       61 aaaaacagaa atgcccactg tctttccggc tctttttatt cttgatttct cttctttttt
      121 gtttcaatag acgattttag ttattaataa atgaaaaagc atttatatta aaatttgcta
      181 ataaaagatc tacatatttt tctagaaatg cttgatcgat ttcatcatac cctttcgtta
      241 ctccgcaatc gatatctaaa acaccgatta aattaccttc ttttaccatt ggcactacga
      301 tttctgattg tgcggctgaa tcgcaggaaa tgtaatttgc atgggttttg acatcatcaa
      361 cgatcaatgt ttcttgtttt tcagctgatt ctccacaaac tccttttccc atcttgatac
      421 gtgtgcaaga aactcgccct tggaacggtc ctaaaatcaa ttcattacca tcgaataaat
      481 aatatcctgc aaagacagtc tcaggcaaag tctcattcaa caatgcagat gaatttgcta
      541 aatttgctaa ccagttatct tctgcttcta ataatccttg ttgctgtaat aataaaattt
      601 cgtatgcatc ttttttctgt ttttctgtcc acatgataaa aaccgctcct ttttgatcta
      661 tggagaagat tatagctcag tttttttgta aacgctaggt tttctcctca aaaaaacgag
      721 atacgtcagg ttaatcacct tgaacgcatc tcgctgtctt tttctattct gcttgttgga
      781 tttctgctgc taaatcagca taatcaatcg tttcgtgaac aaaagtcgga tcatcaactg
      841 gaaaatgctg tacaagggac gagatgcggc taatctcaac ttttccttca gccacttcaa
      901 aatccattac atgtccgcca aatgtatgtg tatcatcgat aaaatgcagg tgaaatcccg
      961 ctgcagcaac tccttggaat aattcaggcg taaagaaccc gactaatgtt ccttggacat
     1021 tgctttgttg gaattctggt tgagactctg aaatttttgc tagtcttctg taaggttttt
     1081 cttgcttcgg catgatacgg atgtgcatat ttttgaataa tccagttatc ttcactgcca
     1141 aaaataggtt ttccccggct tctcttacta atatgtcttt tttcaattgt tctccatctg
     1201 cagtttttga gacagaaaaa ctagtatctg gcgtaaaatc tgtaattgct gcataggggg
     1261 taagttcact tccatctaat tgggcaactg agccatctga tcttccttga taagctacgc
     1321 catctaaaaa gatgacttca ccatcaagcc catgcaatgt tcccaatccc agagttcctt
     1381 tttctaatat atgagcaatc gtttctgttc catccatcaa gccggccatc aaagctccta
     1441 atgtcccgtg ctggtataag attttttcag tcatgtcgac acttccttaa tagaattgat
     1501 ctggtaagat tgtttctccc aatttctcat tatcacggta atcaatcgga atatcaataa
     1561 ttacgggtcc ttctgtcgca aatccttctt ctaaagcttt ttctaattcc tctacacttg
     1621 tagctcggat tcctttagcg ccaaaagctt ctgcatattt cacaaaatca actggaccaa
     1681 agtctacacc tgacgcccgc tgatatttca ttttttcttg gaactctacc atattgtagt
     1741 gtccatcatt ccaaatcaaa tgaacgatat tcaatttttt acgtaccgcc gtttctaaat
     1801 cctgggcaga gaataaaaat cccccatcgc cggaaactga tacgatctgt gtatttggac
     1861 gcaccaatgc agcagaaatc gcccatggca atgcaacacc aagcgtttgc ataccattac
     1921 tgaataacaa atgacgaggt tcataacttc taaagtggcg tgccatccaa atataatgac
     1981 tacctacatc tacggtaaca gtcatatcat ctgttacttt tgactgaagc gtgttaataa
     2041 cttctaaagg atgcaagata cctgcttctc cttttgattg aacaatgtcc cgctccgtta
     2101 attttgcttg aagagatgct aaatattctt ttgcatcttc tgacacttgt tgaggctcta
     2161 atgaacctgt caataaatca agagtggctg agatatcacc gatcaattct ctttcaggct
     2221 gcataaaagg atcgatttca gctggcgctt catcgatcac aatgatccgc gcatcttttt
     2281 ccgcattcca gtttcttgct tcatattcga ttggatcata gccaattgca ataactagat
     2341 cacttcgttt caaaagcata tctcctggtt ggtttctaaa taaacccaca cgaccaaaga
     2401 aatgcgcttc taattcacgt gagatcactc cagctgcttg gaatgtctct acaacaggca
     2461 atgctgtctt ttcaactaat tgcctgatgg ctaacgtttc tttttcactt gatcctcgca
     2521 tacctactaa tagtaccggt agttttgctt ctctaatgcg ttcagctagg taacggatat
     2581 catctgcaga agcagaacct aatttaggat cgctcaatgg cttgatgaca ttaccttgga
     2641 caggtgcatc cactacatct tgcgggatac tgataaagct cgcgcctttt tttgaactct
     2701 tagccatacg ataagcattg gcaatcactt ctgaaattgt ttctggatct tggatttctg
     2761 cactgtattt tgtgattggc tggaacagtg ctgcattatc catgctttga tgggtcaact
     2821 tcaatagatc gcttcgtttt acttgtccgg caattgctaa aactgggtca ccttctgctg
     2881 tcgctgttac caaacctgta gctaagttac tagcaccagg accacttgtt gcgataacta
     2941 ctcctggctc tcctgtaata cgtccgattg cttgtgccat aaatgcagca ttttgttcgt
     3001 gacgagtcac gattaattca ggaccttgat cctctaattc attaaataca ccatcgattt
     3061 ttgcacctgg tataccgaat acataatcaa catgatgatt aatcaaactt tctactacag
     3121 atttcgaacc ttgtttcatt ttttcagcca ttgaacatcg tcctttttat gctttatttt
     3181 taattattag ttagaaataa tcacaaataa gtgatacagt ttctaaacct gtttcattca
     3241 aaagtatagc atgttttatt tcatttgttt agccatatgt ttcaaaaaat gaaaaagaat
     3301 gacagttttt acataaaaaa ctgtcattct ta
//
LOCUS       DAZUKD010000187         3151 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000187 DAZUKD010000000
VERSION     DAZUKD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.193"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG8N9_002794"
     CDS             complement(554..2167)
                     /locus_tag="ACG8N9_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4898321.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
     gene            complement(2308..>3151)
                     /locus_tag="ACG8N9_002795"
     CDS             complement(2308..>3151)
                     /locus_tag="ACG8N9_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4898322.1"
                     /translation="DTHLHLSAAPYFRLLINESANLYAEFAHKSIELDNPSLLFIDKQ
                     QEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFKIDYL
                     KHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIP
                     SNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVN
                     GKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYY
                     LSLK"
BASE COUNT         1018 a          547 c          554 g         1032 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataattattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc t
//
LOCUS       DAZUKD010000188         3092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000188 DAZUKD010000000
VERSION     DAZUKD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.194"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(357..719)
                     /locus_tag="ACG8N9_002796"
     CDS             complement(357..719)
                     /locus_tag="ACG8N9_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898323.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(793..1173)
                     /locus_tag="ACG8N9_002797"
     CDS             complement(793..1173)
                     /locus_tag="ACG8N9_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4898324.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1255..2430
                     /locus_tag="ACG8N9_002798"
     CDS             1255..2430
                     /locus_tag="ACG8N9_002798"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4898325.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2506..3090)
                     /locus_tag="ACG8N9_002799"
     CDS             complement(2506..3090)
                     /locus_tag="ACG8N9_002799"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4898326.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          961 a          590 c          509 g         1032 t
ORIGIN      
        1 agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt tagtaacatg
       61 tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg
      121 ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga
      181 aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa
      241 ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac
      301 aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag
      361 aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct
      421 tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca
      481 aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg
      541 ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt
      601 ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat
      661 tttttagtac catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa
      721 atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa
      781 aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat
      841 ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa
      901 ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa
      961 actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac
     1021 ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag
     1081 tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt
     1141 tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat
     1201 tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc
     1261 gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct
     1321 gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct
     1381 cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc
     1441 actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg
     1501 gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca
     1561 gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca
     1621 ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa
     1681 aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta
     1741 aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa
     1801 ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc
     1861 catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat
     1921 ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc
     1981 aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta
     2041 gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat
     2101 gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca
     2161 gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact
     2221 tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact
     2281 cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat
     2341 gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga
     2401 atcaagtatg ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct
     2461 tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc
     2521 aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc
     2581 gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc
     2641 tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc
     2701 ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct
     2761 tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc
     2821 acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc
     2881 agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc
     2941 ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg
     3001 gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc
     3061 actttttaaa tctagttgtg ggaatgccat at
//
LOCUS       DAZUKD010000189         3000 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000189 DAZUKD010000000
VERSION     DAZUKD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.196"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..595)
                     /locus_tag="ACG8N9_002800"
     CDS             complement(<1..595)
                     /locus_tag="ACG8N9_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4898327.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAE"
     gene            complement(606..1274)
                     /locus_tag="ACG8N9_002801"
     CDS             complement(606..1274)
                     /locus_tag="ACG8N9_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4898328.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1404..1988)
                     /locus_tag="ACG8N9_002802"
     CDS             complement(1404..1988)
                     /locus_tag="ACG8N9_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4898329.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2319..2552)
                     /locus_tag="ACG8N9_002803"
     CDS             complement(2319..2552)
                     /locus_tag="ACG8N9_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4898330.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2567..>3000)
                     /locus_tag="ACG8N9_002804"
     CDS             complement(2567..>3000)
                     /locus_tag="ACG8N9_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFS4898331.1"
                     /translation="KFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKY
                     FSKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKD
                     TNIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
BASE COUNT         1021 a          514 c          390 g         1075 t
ORIGIN      
        1 gttcagctat aacaatagta aatcctttat tttttaaatc acaaagtaag tcagcaagtt
       61 tcttagtcgc tttgaagtcc aaatttgaag acggttcatc caatataatt aatttagagt
      121 ttagcgataa actacatcca atagaaattt tctgccgttc accacttgat agcgaaaaaa
      181 tgtttttatc taatatattt gaaagatcta ataattcagc taattcattg attcttttat
      241 ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat
      301 ttgttgtaaa gaattgtcct ctaggatctt gacttacgta gccaatattt tttgatatac
      361 tttcttgtgt taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc
      421 cgtcataaag tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc
      481 cagaagaacc agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa
      541 gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt
      601 tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct
      661 aataagctca tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt
      721 gttattgcat tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact
      781 aacagaaatt ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt
      841 aaagattctt taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact
      901 gtaatactta acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca
      961 ttgctagtat agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat
     1021 acaatatata aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat agcatcaata
     1081 attttcatta ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt
     1141 ttacgaaaca tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt
     1201 ccctttccta aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga
     1261 ttgatttttg acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgat
     1321 aaaaatgcac cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa
     1381 ttctcaatgc aagctttttt taattaactc agtaattcaa tccacccagg aacagaaaat
     1441 tgatacaatc ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata
     1501 tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact
     1561 aaattttgat tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttc
     1621 gtcacaaatg atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat
     1681 aaaatcaaat cgaatatacg tttattctca aaaatataat taataaatgg atctaattca
     1741 ttgaagttct ctctccaact tttctctata tctatatttt ctaagtttaa gaggtatttg
     1801 gtaaactctt cattatacat attaataagc gcattaatat gcggctgaat aatttcttca
     1861 aataacgctt ccttagaagt aaaatgttta taaaaagcac ccgtagttac gcctgcggtc
     1921 aggcacagtt gccttatctt agtttgctga aatccttctt tttcaaaatg gcttagtcca
     1981 ctttctatta acttttggtg agttataata taatctctgg tttctatggg tacttcctcc
     2041 taataagatg aattaacttt attatcttac acaggacttg aagtttcaat cgttaacgtt
     2101 tattactgaa ctcattcctt agagctaaac taatgagaaa gagcagaata actgatacaa
     2161 aaagcaatcc tacaagaatt aatagactca tctaaatcat tcaccttttc taaaataatc
     2221 cgagaaagat ttaaaaaaat tatatccagc tattaggata agtcctatca taatcactcc
     2281 aatagccatt ttcttatcct ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa
     2341 tcgtcaataa attttttccc tttatcagtt aattgaaatc ctatatctaa atagtcctca
     2401 tatctaagtt tagccagcat ctcacttaca gatgctggac taacacttag aacttttgct
     2461 aaatcattat tttttaattt cttttttctt tttgataatt cctgaattgc tagtaaataa
     2521 attagtatag tagtcgtttt aatttgtacc atacctaccc tccaaattat ttcgaaaggc
     2581 cttcagcaat tttatccagg ttccatttca tcatcgcata gtaactatca ccatcttgtc
     2641 ctttttctgc aattgaatca gtaaaaatcg ttgaatagat aggaatattg gtatcttttg
     2701 atactgtttt cattggtcta tcatccacac tgctttctac gaataaggag ggaacttttg
     2761 ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg tgttccttct tcttcagtgt
     2821 tgatttccca aatgtaagca gaaggcacat tatacgcttt cgagaaatat ttaaagcatc
     2881 cttcacttgt aacaatcatt tttttatcat tcggaattga agcaaattta gatttagctt
     2941 ctttgtctag agaatccaat ttttcaatat acttatctag attttcttta tagaattttt
//
LOCUS       DAZUKD010000190         2957 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000190 DAZUKD010000000
VERSION     DAZUKD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.197"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..443
                     /locus_tag="ACG8N9_002805"
     CDS             48..443
                     /locus_tag="ACG8N9_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898332.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            768..1385
                     /locus_tag="ACG8N9_002806"
     CDS             768..1385
                     /locus_tag="ACG8N9_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4898333.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1426..1725
                     /locus_tag="ACG8N9_002807"
     CDS             1426..1725
                     /locus_tag="ACG8N9_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS4898334.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1759..1926
                     /locus_tag="ACG8N9_002808"
     CDS             1759..1926
                     /locus_tag="ACG8N9_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4898335.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKKYFYCP
                     KCHRRYGVHGT"
     gene            complement(1971..2441)
                     /locus_tag="ACG8N9_002809"
     CDS             complement(1971..2441)
                     /locus_tag="ACG8N9_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4898336.1"
                     /translation="MEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALK
                     ILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKG
                     RPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2447..>2957)
                     /locus_tag="ACG8N9_002810"
     CDS             complement(2447..>2957)
                     /locus_tag="ACG8N9_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS4898337.1"
                     /translation="LYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLD
                     IWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTV
                     KYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTE
                     IKVLMGITA"
BASE COUNT          995 a          468 c          563 g          931 t
ORIGIN      
        1 ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg
       61 aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta
      121 tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag
      181 attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag
      241 taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa gagtctccaa
      301 atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa aagaaagaat
      361 acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct aaattagaaa
      421 tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa agaagtcaaa
      481 ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg gataactagt
      541 ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg aataaatata
      601 atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat gccttgattt
      661 tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt ctgatttaat
      721 ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg agaaaatttt
      781 tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt tccgcgaagg
      841 aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa gattttaaac
      901 gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc tatctacaat
      961 cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga gaattaacta
     1021 aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact gctagttaca
     1081 agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta ctcagctata
     1141 tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta gaagctgcga
     1201 aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa tttcctgcga
     1261 tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat caattgggca
     1321 tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg caccactttt
     1381 cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga cttaacgaat
     1441 caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc catcggtttg
     1501 gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa tttcctggtt
     1561 tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa ggaacaaacc
     1621 gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt cgaattttat
     1681 gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt agtaaaattt
     1741 acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat tgaaataact
     1801 agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt agcaaaaagt
     1861 gtttatcaaa aaaaatactt ttactgtcct aaatgccatc gccgatacgg ggtacatgga
     1921 acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt ctacaaatca
     1981 ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt tgcaatctct
     2041 gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa gcgtttttgc
     2101 ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc ggctttttga
     2161 gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt ttgtgcaata
     2221 taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc atttccagta
     2281 ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg tttcataaaa
     2341 gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga tgggttctgt
     2401 tgcgaagttt gaaaatcaaa cgcgccctct ctgaactcca aatatcttag gctgttattc
     2461 ccattaatac cttgatttca gtagacaccg aaaagccgaa gagcgttcca tttcttcggt
     2521 tctttttata tattcctcga atggtctcca tgcccttaat cgtggaagag gctgtacgga
     2581 gactttgata aaatttattt cgtcgtttaa taggtcgatg gtcttgttct attaaattgt
     2641 taagatactt cacagttcgg tgctctgtct tagtatataa acccacactc tgtaactttc
     2701 taaaggcgga gccaagagaa ggtgctttat cggtcacaat tgctttcggc tcaccaaact
     2761 gtttatggag tcgttttaag aaagcatagg ctgcttgcgt atcccgtttc tttcgtaacc
     2821 agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc caacgtccct
     2881 taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta tttttcttct
     2941 tccaaagata atagagg
//
LOCUS       DAZUKD010000191         2945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000191 DAZUKD010000000
VERSION     DAZUKD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.198"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..498
                     /locus_tag="ACG8N9_002811"
     CDS             73..498
                     /locus_tag="ACG8N9_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898338.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(986..1687)
                     /locus_tag="ACG8N9_002812"
     CDS             complement(986..1687)
                     /locus_tag="ACG8N9_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4898339.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1703..2143)
                     /locus_tag="ACG8N9_002813"
     CDS             complement(1703..2143)
                     /locus_tag="ACG8N9_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4898340.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(2143..2718)
                     /locus_tag="ACG8N9_002814"
     CDS             complement(2143..2718)
                     /locus_tag="ACG8N9_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4898341.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          921 a          580 c          469 g          975 t
ORIGIN      
        1 tctcttccat ccgtttgaag ttttacattc caatggtata atttctttat cctatcaaat
       61 gggggaagta ttttgaagaa attaatcgta ctatttattt tgtctgcggg acttttgaca
      121 ttgactggct gtgatttgtt tggcggctct gaccaatcct cggacgaaac aaccgattct
      181 tctgtcttaa aagcggaaaa agcagatacc accaacagtg tatatacgct aaaagtacgt
      241 aaagatatgg atttatccgc gggacagttg acatttacag ataaaaaaat ggaatggaca
      301 cgcacgtatc agacacaagg aactgattct tctgaatcat ctgaagaaaa aacagtgctg
      361 acagatatca agatcactac caaagatgac gaatatatta tttcaggaaa agaaaataaa
      421 aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc gtttgaagga tgaggatggc
      481 aacatctaca gtctatagtg ccgtctgttg gatcttcttt tgtttctccc cttcttttct
      541 gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg atagtcctga
      601 ttattgtaca tgtaataaca acaaaaatat ccatacagaa tttaatgacc aaggaagctt
      661 gagcatatgc gacaactgtc acaaactagt caaagattca tttaaaacat ttgaatctta
      721 tcctgttcaa aattatgtaa aaagtagaag aagataaaca aatccaagca aaactcacta
      781 tggctgattt ttatgaactt caaactcagc ttcttccttt gatcaagctt catcagtaaa
      841 acaaacagtg gatcatttca gtttacaaat caaagagtaa agatttattt atttcaaata
      901 tttgaatata aaataaccaa acgatagaaa ggcttattct acatcacgta gtaatttttc
      961 tttcccgtcg tttggttttt tatttttagc ctaattcttg accttttgca tgctggttat
     1021 ccgtaaaata ttttccagca ggctttcctt ccaattcagc taatttttct tggatctgac
     1081 ggatcacctt ttttgcatct tctggatttt ctacaaaatc gaatttatcc ccatccactt
     1141 gtattttagg acatacatca aaatcttcat accattgatt atagcgggtg ttcaacgttt
     1201 cataataggt atataacgtc tcatcgcttt ctagctgttc atagtcacgc ccacgttttt
     1261 tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa tcaggccgtt
     1321 tttttggagc aacatcatct aactcgttta gcatcgtatc taataagcta tcgtactgct
     1381 gtacttcgat atccgttacc cgacctaaat cagcgtttag atggaacaac aggctatctt
     1441 catagatgga tcggtctagc acattgtcat cgttcactaa agcatttttc attgctaaaa
     1501 atcgcttgtt caaaaagaag atttgcaata aaaaagcgta ctgctcagga tttgaataaa
     1561 agagcggcaa tacttcgtta tcttcgacat tttcataaaa tggtttagaa tcaatctctt
     1621 ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa atcaaaatca
     1681 ctgacataca acccttcctc ttctataagt actcgcctac gtaagtttca acaggcaatg
     1741 tttcaaaatc atattcacgt acagcttggt ctgattttaa atacgtatgg agactttgtg
     1801 taatcatcaa gttcgtaggt accgtctctt cttgtacaat gatcatcggt tttttcaaca
     1861 tagttgccac acctaattcg taagccgttc ctggatcgat tgtttgatgc tcaaagtcaa
     1921 ggatagcaac aacaaattct gcatttgtca gttcttccat atctttttcg tatacttcct
     1981 gcgcccatcc agcgctgaat agttcataat tgctttcttg gtgatgacgt ggagagtaga
     2041 aacttgtgac tgttttgttt tcctccaacg ctttttcgat acgacttact cgatcgattt
     2101 gctcctctga aaaaaatggt cctgctaaat atatttgact cattattttg cctccttcaa
     2161 taataactga tttttaatag atactgctcg taattcattg accagttcca atggattgtt
     2221 ggaaaagatt acttgatcca accatttttt atccataaaa tcctcttttg caaaagtttc
     2281 taataattgt tgcaagccgc tgtaataacc gccaatattc aatattccga ttggcttgtc
     2341 gggcgtcaga ccgattgcta tccaagaaag aagttgtgag aattcctcca atgtacctag
     2401 acctccaggg aaaacgagga cagcatctcc cttttcaaat aataatacct ttctttcatc
     2461 gatcgtttct gtaggaataa atgtagtaac atcttttcca ggcaattcag tttcaaaaag
     2521 cccattcgga tagatacctg tgacttttcc gttattttcc agtactgttt tactaatcac
     2581 tcccataatc ccggagtatg aaccaccgta aaccagttcg atttcttctt gtgccatgta
     2641 ttttcctaac gtttgcgcaa taaatttgta actttcttta ttaccaaaac gggaaccgca
     2701 aaaaacagta agtttcataa aaaaacactc cctaactcaa aagtctatct tcattctaca
     2761 cgtgtttttt taaaataaaa gactagattt tgtagttttt tagtggtata taggatagac
     2821 gccactatat atagtattta gaaaaaaatc atttttcagc cattttatag ataaaacaaa
     2881 aataaataac gtgttttcat cgatatgaaa tgacttactt atagaagata ctgggatttc
     2941 gtcct
//
LOCUS       DAZUKD010000192         2840 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000192 DAZUKD010000000
VERSION     DAZUKD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.199"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..400)
                     /locus_tag="ACG8N9_002815"
     CDS             complement(101..400)
                     /locus_tag="ACG8N9_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898342.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(403..564)
                     /locus_tag="ACG8N9_002816"
     CDS             complement(403..564)
                     /locus_tag="ACG8N9_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4898343.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(579..938)
                     /locus_tag="ACG8N9_002817"
     CDS             complement(579..938)
                     /locus_tag="ACG8N9_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4898344.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(935..1786)
                     /locus_tag="ACG8N9_002818"
     CDS             complement(935..1786)
                     /locus_tag="ACG8N9_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4898345.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1803..2636)
                     /locus_tag="ACG8N9_002819"
     CDS             complement(1803..2636)
                     /locus_tag="ACG8N9_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4898346.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(2643..>2840)
                     /locus_tag="ACG8N9_002820"
     CDS             complement(2643..>2840)
                     /locus_tag="ACG8N9_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4898347.1"
                     /translation="IDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKN
                     KYQIKGIKLNQETIKKLRIGG"
BASE COUNT          685 a          617 c          423 g         1115 t
ORIGIN      
        1 aacttaacta attccgctcg ttcttttgct tttttatctt tctgaatctt catcatgatt
       61 ttcttgagga tgatatcact gtattgtgta atgagatcca ttatttctcc tccacatacc
      121 taaactgtcg cccctttgaa tcaatccata agctcctagc tctatcccag atgatgtttt
      181 tgctcagtcc agtaatttca gataactgtt cagctgtacc cgttactaga attcgatcac
      241 catgccagat tgcaattttt ctcggcgttt tccgtttagg cttttcagtc cacattgatt
      301 taccgagctt ttggacttct gcaactattt ctttgtcttc ttgccaattc tcagaatgtg
      361 tcagttcaat gattcgtttc attgctgctt tcttacccac gctcattcct ccaatctacg
      421 aatttccctt cttaaattca ctatgtgcaa attgattgcc tttctcgccg tttcattgac
      481 catcactgcc tttatccgtt ccagatcgtc aatctcacgt tgaaggcttc gaatacgcat
      541 ttgaatcact tcttctgttg tcatgatgga ccacctcgtt aaaaacgctc ttccttgaac
      601 gtattccgat attttttagc taatatcaac ggcacttgat attgatgaca gaacaacttt
      661 gccttgatct taaagtcttt tgtctgcatt cctttgacat ctacgacttt gacaagtttg
      721 ccgtttttat aaaatgtgaa gtcgggaata tactcgatct tgcgatactt ttttccatct
      781 agttcaaatt tcggcatcag ctcaaatctt tcctgaagtt ttactttcca gccgttcgct
      841 tctgcttgcc acaaggctaa atcgtaatac tctgcttccg cgatagaatc aaacttgata
      901 cctcgatgaa cagttttctt attacggtat ttattcatgc gatactacct ttcactggtt
      961 ttatgcgctt gtctgctgtt tgttggaatt tcagcgcata accttctgaa ttcttaaata
     1021 ttctagaaac aattctttcg ccgtaggctt ctcttagttc aggaccggat aagtttgttg
     1081 tgatgatcgt tgccttgttc tgtctggctt ctaagagcgt gtttaacgtg ttgtttgtaa
     1141 actgcctact atttgatacc ccgctaccta attcagctcc aatatcgtca aaaaccacca
     1201 aatcagttgt tttgatatcg gctataagcg atccttcaat ttcttttctc agttcagcat
     1261 tgttataaga aaactttatt tgctctaata actcttgata gcttataaaa agtattttct
     1321 tgtcataatt tgagcgctca agtatttccc aagctgtcgc cattgacaag tggctttttc
     1381 cgcttcctga tttccctgat agaatgaaat gtgcaggatg gttcagtaag acatcattta
     1441 caaagctttt agctctttct aaagcaattt tcgtttcttg gtccactacg tgataattct
     1501 ccattttgca tttaaacaaa gttttatctg ttaataccga accattttga aaaaaactca
     1561 acgctcgtgc ttttaagctg tcgttatata tccgttcggt ctgtatatcc tctttcacac
     1621 gtaacgcttt ataaccacaa ctcatgcatg ttggtttaca acgttctgaa ccatccttat
     1681 ttttagctcg ccaactatac aaaggttcgc tacattctgg acatttcccg ctttgcacta
     1741 atactcttct tattagtttc tccatagcat ttgctaggct ttccatgtga tgcatctcct
     1801 ttttaaattg gcaagtcgtc atattcacta ggattgctgt actgtagttt ttgactttgc
     1861 tttttatgat tattcttgtc tgctttgatt tcgaatttga gcttctcaaa tttttctctc
     1921 aatttcttag cacttctaat atttccaaac caaaattcat ttgtaggtag ccaattgatc
     1981 acatactcaa tcgcttctat agacgcttta tctctttctt ccatcaacct gattgtgtct
     2041 gcccattttt cgatatctac tttgttcatt tcttttggaa aatcttcagt taaattactt
     2101 tgcaattttt tagcaaggcg taagtgttcg tcagaatact tacctttctt ttcttcttta
     2161 tctatatcta tatctttctc tatctctatc tctaactctg gtgtagtttt gtctggacat
     2221 ttgtccgaca cttgtcctcc agttattaaa ttccgttttg cctcttctat tttctttctg
     2281 tattctcttt ttctatctgc ttcagttgag gattttccaa tgaaactttg tatatcagac
     2341 atataaattg ctccgttatc taatacgtca ataagctgca aatcacggaa aatttgtacc
     2401 gctttttcta cgactcctac agaatgtctt gtaatagttg cgagcattgt agagttaaat
     2461 ggaatcctgt cattaaacat caacttacct tcgtgtttta gacttcttaa ataaagtttg
     2521 agaagaatat tagaataaat atagccatct ggcatacttt ctaagagaac catctcgtca
     2581 ctatcgaaaa aattctcttt tagttttaaa tagtagtagc gtttgttgtc agacaatatt
     2641 ttttaccctc ctattctaag tttcttaatt gtttcctggt ttaacttgat ccctttgatt
     2701 tgatatttat ttttgaaatt gatcacacct atattgtgtt tctccgtgtg atgaatcctg
     2761 cagagtgctg caaatgtgta ctctgaatga tcaacttctt tgcgctttcg tcttcctagc
     2821 gctttgtcaa agtgatcgat
//
LOCUS       DAZUKD010000193         2814 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000193 DAZUKD010000000
VERSION     DAZUKD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.200"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..254)
                     /locus_tag="ACG8N9_002821"
     CDS             complement(<1..254)
                     /locus_tag="ACG8N9_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4898348.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="ACG8N9_002822"
     CDS             complement(244..1443)
                     /locus_tag="ACG8N9_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4898349.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..>2814)
                     /locus_tag="ACG8N9_002823"
     CDS             complement(1573..>2814)
                     /locus_tag="ACG8N9_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898350.1"
                     /translation="ETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSID
                     SYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAY
                     KLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIK
                     GLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSV
                     IKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFL
                     WMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILN
                     KVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVS
                     LIISSFNILRRNPKELLIDNN"
BASE COUNT          862 a          545 c          425 g          982 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      421 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttc
//
LOCUS       DAZUKD010000194         2712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000194 DAZUKD010000000
VERSION     DAZUKD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.201"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..624)
                     /locus_tag="ACG8N9_002824"
     CDS             complement(<1..624)
                     /locus_tag="ACG8N9_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898351.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPL"
     gene            729..974
                     /locus_tag="ACG8N9_002825"
     CDS             729..974
                     /locus_tag="ACG8N9_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4898352.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1130..1981
                     /locus_tag="ACG8N9_002826"
     CDS             1130..1981
                     /locus_tag="ACG8N9_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFS4898353.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2014..>2712
                     /locus_tag="ACG8N9_002827"
     CDS             2014..>2712
                     /locus_tag="ACG8N9_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFS4898354.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDS"
BASE COUNT          992 a          376 c          467 g          877 t
ORIGIN      
        1 aaggggcgtc tccaaataat ctgctgtata ttcatagtca acgtaacctg ctaatacaaa
       61 agagagtatc cctttattta atgttagatt taatggataa tcatacgact ttattttttt
      121 caacttcata ttgtacatat cttcagccat aaaagttgag ttcgtgattt catcttgggt
      181 ctcattaaaa aagttatccg aataatcgct tatttttgaa aagtctttat tggcaccagc
      241 ctcaaaaaat tgttcaactt tttcagtata aagttgtgct aacgctcctt tttcaatatt
      301 aactttaata ttttcatgtc tatcaatttg tgtgctgttc agtaagggaa gatcagcttt
      361 tagttttata tcgtcttgat ttctttcaat ttcaattggc aaaatctttt cagtagattc
      421 agtttctgat aaaggaacat ctagtttttt atcatgataa taagcttcaa atccatctgg
      481 aaccggaaaa aaatgtattg ggtaattctt ttctgaggtt gttgtactag tttccgtact
      541 tgtagaactt gtgtcagaag tggtacaccc gcttatagtt ccgatgagta aagcgcaagt
      601 tagaatgatt gcaaatttct tcaaaaaaat tcctccttaa aattgaatat ggactaatac
      661 tagtcccatt gtagcatacc gaatctttaa gattaataag aaaaaggact aattttagtc
      721 caaaaggtgt gctattcttg aaagaggaaa aaattattga taaagaaaat gtaatcggta
      781 gcaatattag acgagtccga ttagaaaaag gaattggtca aaccgagctg attagagacc
      841 tacagcttcg taaaattgct ataactaggg aaacgttggt taagattgaa ggtggccgcc
      901 aacacattaa gttggatcaa ttgaaagcca tcaaagatat tctacaggtg tcttatgaag
      961 atttgcttca atgactactt gtagtcattt cgtttgaaat tattatgtta cacccttatg
     1021 tgtcacgaag aaatactggt ataataatga atgggatatg taacgatagg gtatactgtt
     1081 atgttgccta ataaattgtg gatcaaaata caagaatgga gggatatcct tgtataaggc
     1141 atatgaattg gattttacaa atgctgactt aaatgcattc tcctcaagct cgcaatcata
     1201 tacacatgta gtaaatcaaa tagatcacaa ccaaaaatct attaacaaga gaatagaaaa
     1261 tctatttgaa agtgaaaatg atttagttga ttattattct aaggacagca aaattctaga
     1321 tgcagacgaa attattaaag attggtttcc aactataaaa gcagatattt ttatatctca
     1381 ctctcatgct gatgaaaaat tagctatacg ttttgcatca tggctatttg aaaattttgg
     1441 tcttactgca tttattgatt cttcggtttg gggatacagt tctgatttat taaaaaaaat
     1501 agaccaaaaa tattgctata aggaacaaac aaaaacatat gattatgata aaagaaatgt
     1561 aacaactagt catgttcata tgatgctttc tacagcttta aataatatga ttgatagtac
     1621 agaatgtctg ttttttctta atacacctaa ttcaattagt ttgagcaatg aaatcactaa
     1681 tgaacagaaa tttacgtatt cgccatggct atatagcgaa ctgacgacag cttcaatagt
     1741 ggaaaaaaag aatccacgat tggaaagtaa tcctcaaatg agcaccgagg atgttagaag
     1801 cattataaag cactattctt cgcaagaaaa tctgcaaata aaatattctt taaatttaag
     1861 tgaattaact tatttacgaa aagctgattt gaaaaaatgg gctttagatg ctgtggaatg
     1921 cactaaagga agtaatacat tagatatttt atataaaaac accattttca ctaatctcta
     1981 atcaatttac tgaatcgaaa ggaaccgggt agaatggata agaaagtttt aataaagagg
     2041 ttctctgaag ctattgaaaa aggaaatgca gcaatatttg caggtgcagg attatcgatg
     2101 tcacaaggat atgtaagttg gccggaacta cttaatgatc ctgcaacaga aatcgggttg
     2161 gattctaaaa aagaaacaga cttagttact ctggctcaat attataaaaa tgaaaatggt
     2221 ggatcaagag gaattttgaa tcagatactt atggataatt ttggtgaaga attagaaata
     2281 tctgaaaatc atcgaatact agcaagttta cctattgaaa cttattggac aactaattat
     2341 gatcatctaa tagaaaaatc aatccgagaa gcttataaaa accctcaggt taagaaaaat
     2401 tatactcagt tagcaacaac taatccaaat gtggatacaa ttgtttataa aatgcatggt
     2461 gatatagatg atgtcagttc gacagtaata acaagagatg attatgaaaa atatgatgat
     2521 gattcgtacg cgttatttaa agaaacttta aaaggagatt tgttaacaaa aacttttctc
     2581 tttttaggat ttagttttac agaccctaat ttagaaagaa ttttatcaga tataagatgg
     2641 gttttgagag aaaaccaaag acctcattat tgtataatga gaaaaatatt aaaggaaaat
     2701 tttgtcgatt ct
//
LOCUS       DAZUKD010000195         2657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000195 DAZUKD010000000
VERSION     DAZUKD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.202"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..1040
                     /locus_tag="ACG8N9_002828"
     CDS             255..1040
                     /locus_tag="ACG8N9_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4898355.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1053..1904
                     /locus_tag="ACG8N9_002829"
     CDS             1053..1904
                     /locus_tag="ACG8N9_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4898356.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2028..2480)
                     /locus_tag="ACG8N9_002830"
     CDS             complement(2028..2480)
                     /locus_tag="ACG8N9_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898357.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT          844 a          444 c          545 g          824 t
ORIGIN      
        1 tcttcggaaa taagccgaaa tttcacaaaa atttgagaag taatttttgt aaattacttc
       61 ttatttcttg aagctggcca cttctgtcac aacctctttc tttatagaaa taaaaggaca
      121 ctagcaattc atgaaaacga ttgctaaagc gtatcatttg ctttagacta tatatcttgg
      181 tgtacactca ctttaagacg gaaataaaag ttatccgtaa taacaggttt aggtatgaaa
      241 aaggagtggg tactatgtat aaggaattag aaaacaaagt cgcagtagta actggtggct
      301 caaaggggat tggaacagcg attgctcaac gattcgggaa agaaaaaatg aatgtagttg
      361 tcaactataa ctcggataaa gaaggagcag aaaaagcagt caaagcaatc gaagaggcag
      421 gcggaaaagc agtctctgta caagcagatg tcagttctga agaaggaata cagcgtttac
      481 ttgatacggc tttagaaaat tttgggacac tggacgtttg gattaataat gctgggatgg
      541 aaaatcagca tccaacccat gaacttagcc ttgaagattg ggaaagagta ttaaaagtca
      601 atttgactgg cgtttttatc ggaacgaaag cggctttaaa ttactttaca gagaatgata
      661 aaaaaggaaa tatcatcaat atgtcttctg ttcatgaaca aatcccatgg ccaacgtttg
      721 ctcattacgc tgcttcaaaa ggcggagtaa aactattcac cgaaacggta gcaatggagt
      781 acgcaccaaa gggaatcaga atcaatagta taggacctgg tgctatccgt acacctatta
      841 atgcggcaaa atttgatgat cctgaacaaa aagaactctt ggaaagtatg atcccactag
      901 gacgcgtagg tgaaccaaaa gaaatagcgg cgacagctgc atggttggct tcagacgaat
      961 ccagttatgt tacaggtatt acactatttg cagatggtgg gatgacttta tatccttctt
     1021 tccaaggtgg aaaaggataa gggtgaataa aaatgggttt attgattgca ttgatcccgg
     1081 cagttgggtg ggggatccag ccactgattt tgaaaaagat tggtggcaga ccaacaaatg
     1141 agatattagg aactggtatt ggagccgtgc tcgttggctt acttgttcaa ttattcgctt
     1201 ctccagctgg tatttcagtg actacatttt tgattagttt actttctggt gcattctggg
     1261 tcatcggaca aacaggacag tatacatctt tcaacttgat tggtgtttct aaaacgatgc
     1321 caatttcgac tgcattacag ttagtcggaa cctcattgat tggtgtcttc gcttttggag
     1381 aatggtctgg aagtatggga aaaatcattg gtgcagcagc aatcattctt cttgtatttg
     1441 gttctgcttt aacggcaatc agtgatgatc ctggaaaaaa aggcggtatc acaaaaggaa
     1501 tcagtctatt agcatcaaca agtattggtt attgggttta cagtgcattg cccaaactag
     1561 ttgatgcaag cggactttcc attttctttc cgcaaatgat cggtgtcttt ttaggggcag
     1621 tgatttatgt gattttccga aaaccaaccg cattttctga agacaagagt tggaaagcaa
     1681 caatagttgg agccatcttc agtgtgtcag cattggctta tattttttca gcaaatgaaa
     1741 atggggttgc tactgcatat atcattacac agctaaacgt agttatctct acattaggcg
     1801 gtatgtacat cctgcatgaa aataagacac caaaagaaaa aaaattaacg attatcggat
     1861 tattgctgat cgtagtagga agcatggtca cggtctttat ttgaggataa agatggtaaa
     1921 atctcattta ttaaaaaaat atttgtttga accaaacagt aagtacttat aaaaaaatag
     1981 cgctagaatt tccaacatct ctggaatttc tagcgctttt taattgttta agatacaagt
     2041 gaaatatctg tctgcatcaa taatttttca ttgatccatt gactaaaatt tacgacatga
     2101 ttgaagttac caccgattag tatctccgca tgattgtctt tataaagatc gtattgtaaa
     2161 agacggtctt ttttatttgc gtaaacatcg ttatggtact ggactaattg ttctcggatt
     2221 tgattgattg ctgtaaaatc acgatactga aggttgaaac gcatcgtgat tggtgcattt
     2281 agcatgaatc gctgcatttc ttttgctgct tcctctgctt gatctccgta taggaactca
     2341 aaaagattac gttcagaaaa tttccattgc ccgccaacct ttgttccttt tagttttcct
     2401 tcatgaagat aatttctgat agaacgtgta gtaacaccta attgttcggc taattcattt
     2461 actgagtaaa aatctttcaa tgttcaccct cctatgtgaa tggttctact gacttagttg
     2521 atatttgctt aatcacgaaa tgtagaaaga acgattttaa ttaaggtcat tgtaaaaatc
     2581 aagtcagaaa attctaactt ttctttctct aaagaaattc gttctctcct aaagtgatat
     2641 tatatttaaa tatacct
//
LOCUS       DAZUKD010000196         2608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000196 DAZUKD010000000
VERSION     DAZUKD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.204"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8N9_002831"
     CDS             14..1141
                     /locus_tag="ACG8N9_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4898358.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG8N9_002832"
     CDS             complement(1359..2501)
                     /locus_tag="ACG8N9_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4898359.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..>2608)
                     /locus_tag="ACG8N9_002833"
     CDS             complement(2506..>2608)
                     /locus_tag="ACG8N9_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS4898360.1"
                     /translation="LLVEKGYFFYRNSKIKRIPISILEEVLHISWEE"
BASE COUNT          873 a          471 c          421 g          843 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagt
//
LOCUS       DAZUKD010000197         2507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000197 DAZUKD010000000
VERSION     DAZUKD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.205"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8N9_002834"
     CDS             complement(<1..430)
                     /locus_tag="ACG8N9_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4898361.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            774..1181
                     /locus_tag="ACG8N9_002835"
     CDS             774..1181
                     /locus_tag="ACG8N9_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898362.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1195..1596
                     /locus_tag="ACG8N9_002836"
     CDS             1195..1596
                     /locus_tag="ACG8N9_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898363.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1598..1969
                     /locus_tag="ACG8N9_002837"
     CDS             1598..1969
                     /locus_tag="ACG8N9_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898364.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(2005..2307)
                     /locus_tag="ACG8N9_002838"
     CDS             complement(2005..2307)
                     /locus_tag="ACG8N9_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4898365.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          901 a          366 c          401 g          839 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcatttttga ggggaagtac atatagttgt tgatatagtt gctaatattg ttagctaatt
      541 tgttagtaaa actgtatacg aatttagata gtaaaaaaac agtttgttcc ttatatagag
      601 gcacttttac tactgattaa atgtcagata tgcttacaaa tatacttata aatatgctta
      661 caaaattagc taatatttcc gttgacaaag ctttgttact attgttaaac taaattgact
      721 agcaaaagtg aaacataaat ttattaaata aatgtaaaaa ttaggagaaa tcaatgtctt
      781 atagagtaca attaataatt tcagaagatg tagaaagtca gcaatttggc actaatgtta
      841 ttaataaagt aattaatcca gctctttcta ttaatgcgcc tttaattcct actgctttat
      901 cattttctgt aacagctgtg gtgtctgaga tagaggatac agaaaagata aaaatagtag
      961 aaattgaagt tttaaacaag aatgaaaaac aaattttttc tacgggtgaa gtatcggtaa
     1021 gcttgcctcc tcaagttaac gatataaact ttaatataaa cgccagaaat gtcttggttg
     1081 aagaagcagg agagcattat gctgttgtta aatttaatgg aactgagatt ggaaggcaaa
     1141 tctttgatat caaggtcaac aaaccagtgg aaaaaaatta aggagacgat acggatgctt
     1201 gatatagtac catcaaatac acatcggagt ggtagcttaa taaattttgc tgcgattttt
     1261 gcttgcgcat cggtaattct agcatctcca gttgttgtgg atgcaagttc tactccgaaa
     1321 actaaaaatg ataatcaact tgtaattact acaaaaaatg agattaacac aatttcaagt
     1381 aatgatggca aaatttttga tgtgataaat acggttttaa aaagaaaagc tgaaatcaat
     1441 ataaaatatg atgaagattt aaacttgtat ttctttgtta tcaaaacaac atctgaattg
     1501 ttttcctcag attacgatgt tttggatact ctcgataatg tattatctga ctataaatat
     1561 atggggaaat ctgtagtagc aactttgggg gaataaaatg tttgacttta atgaatattt
     1621 atctatttcc aaacaaattg aatcagatga taaatataat tctaaagaat cttatagaag
     1681 aactgctgtt tcaagagcat actacagtgc ttttaaaaaa tctgatgagt atttgaaaga
     1741 aaattatgat attatttata atggaagtag cggaaaagga agccaccaaa ccgtttggaa
     1801 tttgttttca acagttaagg aattgaatac cttaggaata cataatagtg ggtatagaat
     1861 gttagagaaa agaaaatgtg ctgactatat tcctaatgaa accattacga aaacagatat
     1921 ggcgctgatg aatcgagaag cagaaaaaat aataaacaaa ctcacctaat gaatttcaag
     1981 cgagtttgtt tgttattttt ttatttaatc tttgtatctt tctggatcaa cgaaagtata
     2041 ctttatatag tcataacgcc gatgatcgct acgtgcgtcc ggcacgtcag tcacgatatc
     2101 aaacaaaaag tatacaccct tcttcattct agttttcgca gcaggaattt tgaaatagtt
     2161 cttattagaa tagtagagat tgattaataa gctatcttcg attgctaaaa agaaaacttc
     2221 tgaattccat actttataaa aatctttgat aaatctattc gaagggtcaa atttaaacca
     2281 taattgtgtt tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa
     2341 tgttcttttt gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc
     2401 aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat
     2461 attaagattt cctgcaataa caaacacaac agaagacgtg taatagt
//
LOCUS       DAZUKD010000198         2480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000198 DAZUKD010000000
VERSION     DAZUKD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.206"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..>2480
                     /gene="esp"
                     /locus_tag="ACG8N9_002839"
     CDS             154..>2480
                     /gene="esp"
                     /locus_tag="ACG8N9_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4898366.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          896 a          383 c          500 g          701 t
ORIGIN      
        1 gaaatgtact ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa
       61 ggttagtaag attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta
      121 ggaaagtaat tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta
      181 tttctagaaa aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg
      241 gttgcatctg ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca
      301 caagatgaaa tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt
      361 gacaaagtag cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac
      421 ttttcggatt tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca
      481 attggatttg aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc
      541 cgtcttcaat tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag
      601 aaaggaattg gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct
      661 acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa
      721 acaaccgata cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa
      781 atttttaaag agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac
      841 ttggtaagtc atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt
      901 ttaacagatt cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag
      961 tttaaacatg gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt
     1021 tcaggatcca caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat
     1081 aatttctcat attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat
     1141 gaacgattag ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta
     1201 acatatgatg tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg
     1261 gaaggtcatg aaaattatgg cataggttca ataactaata atgatttaac taaacttatc
     1321 gattttgcta atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag
     1381 ccattagatg aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca
     1441 tttggtgaag atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag
     1501 aacacttatg gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac
     1561 gataaagaag agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta
     1621 tatgatgttg acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt
     1681 cacaaagccc aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca
     1741 aatgaaaaag atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat
     1801 tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat
     1861 aaaccagaag aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc
     1921 gtggtagttg gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct
     1981 gaagatgcag agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg
     2041 aaaggtattg taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct
     2101 gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca
     2161 gaaacgacag ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa
     2221 gatgcctttt caatttcaga tgtctataat aagtacttcg gtaatgcaag tgttgatgca
     2281 aacactggta ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact
     2341 ggaacaattc ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca
     2401 gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac
     2461 aaagtaacct tcaccgcagg
//
LOCUS       DAZUKD010000199         2458 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000199 DAZUKD010000000
VERSION     DAZUKD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.207"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8N9_002840"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8N9_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8N9_002841"
     CDS             1320..1778
                     /locus_tag="ACG8N9_002841"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4898367.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG8N9_002842"
     CDS             1937..2407
                     /locus_tag="ACG8N9_002842"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4898368.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DAZUKD010000200         2432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000200 DAZUKD010000000
VERSION     DAZUKD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.208"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..485
                     /locus_tag="ACG8N9_002843"
     CDS             3..485
                     /locus_tag="ACG8N9_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4898369.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            522..1532
                     /locus_tag="ACG8N9_002844"
     CDS             522..1532
                     /locus_tag="ACG8N9_002844"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4898370.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1647..2360)
                     /locus_tag="ACG8N9_002845"
     CDS             complement(1647..2360)
                     /locus_tag="ACG8N9_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898371.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          820 a          402 c          497 g          713 t
ORIGIN      
        1 aaatgattga cttggcaaag aaattcttac acgaaaatat atattttgag ttgtatcatc
       61 atcgagccat ttatacaaat gaagatgctt tggtaataaa gaaagaacaa ggatttacag
      121 gtacagagac caaaagctta ttcttaaaag ataaaaaaga taataactac gtatatctga
      181 cttttactac gaaacaaacg gatttcaaag cattagggaa gatcgtcggt aaaaggcttt
      241 ctattgtttc atcagaacgc atggaagaac aaacgggaca aaagcagggc gctgtttctc
      301 cgtttggtta tgaaaaacag attccgatta ttattgatga agagctctta tcaaatgaaa
      361 aacttgtttt tgcaccaggt agaccagatc aaacgatggt catccaaacg gttgacttaa
      421 acaaaatcat ccagctactt gagatgccga tatttttgat gccggcaaac gaaaaaaaca
      481 attaaccaag taaaatagtg catacttgga ggtgaagcaa catgataagt tatccattat
      541 tagaaagaaa tgcaacgatt ggggttactg caccatcctc aggcgtatca gaggaacacc
      601 attcgttact cgaatcagct ttaaagagaa tggaagaaaa atctttccaa gtagttactg
      661 gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg
      721 aattcaatca aatgatgcaa gatgaaaata tccaaatgat ctttccgcct tggggaggag
      781 aattattgat tgagattctc gattttgtcg attttgatgc tctacaacca aaatggatag
      841 taggttattc agatattagt acgttgctac ttgctgtcac attaaaaaca gggatagcca
      901 ctgcacatgg aaccaacctt attgatttaa gaggagagta cttggatgaa acgacagcaa
      961 tgtgggaaag tgtactgaaa acaagagaag gagaatcgat tctacagcaa ccttctgaaa
     1021 agtatcaaaa agagtggccc catgagcatt catcatcttc tgttttccat ctcactgaac
     1081 caacaaattg gcaatcagtc agcggagatg cagtaaggat gaagggacga ttattaggtg
     1141 gctgtattga tacgatcagt catttaatcg gtacccctta tggagatgtc caatctttta
     1201 aaagaaagta taatgatgga aagccgatct tgtggtttct agaaaattgt gagctatcgt
     1261 cagttgattt aagaagatca cttgttcaga tgaaactggc aggatggttt gatggcagct
     1321 cggggatcat gttcggtaga agtgcagcag aggatttgtt agattataaa atgaaggatg
     1381 tatatgaaga tctgtcaaag gaattaaaac taccgatctt gtatgatatt gattgtggtc
     1441 atgtaccgcc acaactgaca ttgatcaatg gtgcctatgc agaagtagcg gtagaagata
     1501 gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt ggagtttatc
     1561 aaagaccaac aaatctaaat agaaaagaaa atgaaagaag ctgacagtat tttgccagct
     1621 tctttttgta tgatctatta gaaattttaa ttatccataa ctatcacatt gatcagctgt
     1681 ttcacatcat ttggattacc tgtaggaata gctactacag atgcattatc aataatttct
     1741 tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat ttgccaatgt
     1801 gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac taaaactccc
     1861 tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc actccctgca
     1921 tcataagggg cgataggtgt tcctgcagga taatgaataa catgtagttc gaaatcaccc
     1981 gatgtatctc taaaagtatt acctctaatt gctagccaaa cacgtgcgta ctcaatttgt
     2041 agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga agttgtgtct
     2101 attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga agtattgggc
     2161 tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga tttctgagta
     2221 cttgattttt cagctgaact ggtttgtttg attgaacttt tgtctgtggt cgcagatgga
     2281 tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac caacaataaa
     2341 ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca gtatagcata
     2401 atcctgaatt tttgaatccg taaaaccttg tc
//
LOCUS       DAZUKD010000201         2430 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000201 DAZUKD010000000
VERSION     DAZUKD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.209"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..295)
                     /locus_tag="ACG8N9_002846"
     CDS             complement(11..295)
                     /locus_tag="ACG8N9_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898372.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(995..1090)
                     /locus_tag="ACG8N9_002847"
     CDS             complement(995..1090)
                     /locus_tag="ACG8N9_002847"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4898373.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            1743..>2430
                     /locus_tag="ACG8N9_002848"
     CDS             1743..>2430
                     /locus_tag="ACG8N9_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4898374.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLP"
BASE COUNT          791 a          389 c          454 g          796 t
ORIGIN      
        1 tttctcatgg ttatatcatt cctccgataa gcatttcaat gatttgatta tttgtgtaga
       61 catactcgct taatatttga gtattttctg tttggatagc aataaattta tcgagcaata
      121 aatcgaattg gtcttttttg taagtcgtaa gttgacggtc aaaatcactt tcgattaaaa
      181 cttttagata tttatttcga ctccattttt ctcctgtttt attcgtgagt tcttggcaac
      241 gtttgtctat tgttgcaaca aactttggat caatattccg aactaaaatg tccataaaaa
      301 atctcctttt gcaattatgt ataatttatg tataccatac aaatactata tttgtatagc
      361 tgttttttgc caaaatgcta tacataagct acccacgatg gcgaaaaagt gatatcaaat
      421 ttttttctga gaaacgtgat aggcacagcc tatctaccca ctttttttag aaagtgggta
      481 cacgtttcct tatgctcttt aaaattcggg ggggagtgct gcacgtgtaa tcgataacca
      541 aatttaacag acgaaaaatt gttggaatat aaaaatatga cacccgaaag aaaacgatcg
      601 ttggaaaaat taatgcaact agtgaacgaa gacggcggaa tttcagacgc agcttttgac
      661 gcatggagcg aatttcatgg agaatataat taagtgggtt taaatggctc taaaatcgct
      721 tctaagagtt ttttagcttg gaccaatgaa atacaccaaa aactaaataa aacgtcttaa
      781 atcgaaaaat acgagctctc agagcgactt tatttgtaaa tgttgcatat aagaaataat
      841 tacggatttt agacttttgt aggatatttt tgaaataaat atttatttct gttataattt
      901 gattgcaaat aagtgaacac gaaacacacg aaatccccag agaggtcgga ttctctgggg
      961 ctttttttgt gggctgagtc gccttaatta agttttactt cttgtttcgg ttattcaacc
     1021 agtaactgaa caaagcgctt aaaagcccaa caagaaggga gcaaataaag ttagtgaaca
     1081 cgaaacacac ctccttccag tcgcaagact gtcggatagg cgacaatcaa ttatacaata
     1141 aaaaagcaaa aataagccta agttttttat tgccttgggg ttgttagggc ttgccctgac
     1201 cgcctgtaag gcgctttaga acgaaggaca tagtttttat cgaatcttgt cttagaacgg
     1261 cttagacgag ctctcaggac gccacaaagg agtccctaat gaattatttt tggcgaagga
     1321 gcctaaaggc gactgctgca agtaaaatgt gagagcgtag cgatcactcc ttcttacagc
     1381 gaccataggg agctagattg gcagtttaat atcatgcgcg aacctgttta ttttaaaaag
     1441 tgggtgattt tttggcggga aattttgctc gcgcgcgcgc gtttatttta tttatttttt
     1501 aaagattttt tagaaccctt tttagggggc agttgagcct tactctccca acggatacag
     1561 aagggtacta acaacttttt atctgagtac taacaacttt ttatctgagt actaacaact
     1621 ttttatctga gtactaacaa ctttttatct gagtactaat atccttttgt ataataacaa
     1681 ccaacctatt tacgagttgg ttgttaaagg ttattattta tttgtaaggg gggaagaaag
     1741 gaatggatga aaaaacggaa ttaacagtca aatatcaaaa tgaattgaat ttagtaccgc
     1801 tgaaaaattt caatgctaag cagatggatt tattttttgc attatgcgct cgtatgaaag
     1861 atcgtggttt gaaaaaagtt agattttctt tcgaggaatt aaaagagtta agtgattata
     1921 agatgactgc tacaaaagcc tttgtcggtg atttagaaaa gctctataaa gatatgctta
     1981 atcttactta ccgtactgaa gatgaggatt ctattcgcta ctttgttttg tttactggtt
     2041 ttgaaatcaa taaaacaaaa aagtttgttg aagtcagtgt taatccggac ctagaatata
     2101 ttattaatgg tttaacagct gaatttagta gatttgaatt atctgctttc acgtcgttgc
     2161 gttctacgta tgcaaaaact ctattccgac tgcttatgca gtaccgttct actggttatt
     2221 atgtagttcg tattgaagag tttaggagct taatggatat cccagaatac tatcaaatgg
     2281 gtaacattga tcaaaaagta ttaaatcctg cattacgaga actaaaaaaa tacttcaatg
     2341 atcttaaagt cacaaaaata aaagcaaaaa agggcaataa aatcgccaaa ttagaattta
     2401 ctttttcaag cctaacgtca gatttgccaa
//
LOCUS       DAZUKD010000202         2395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000202 DAZUKD010000000
VERSION     DAZUKD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.210"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8N9_002849"
     CDS             316..570
                     /locus_tag="ACG8N9_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4898375.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8N9_002850"
     CDS             571..888
                     /locus_tag="ACG8N9_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4898376.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8N9_002851"
     CDS             1447..2382
                     /locus_tag="ACG8N9_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898377.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZUKD010000203         2387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000203 DAZUKD010000000
VERSION     DAZUKD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.211"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..223
                     /locus_tag="ACG8N9_002852"
     CDS             11..223
                     /locus_tag="ACG8N9_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898378.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(501..2300)
                     /locus_tag="ACG8N9_002853"
     CDS             complement(501..2300)
                     /locus_tag="ACG8N9_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4898379.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          828 a          439 c          448 g          672 t
ORIGIN      
        1 agggaatgaa atggtaaaag cagaagtagg aagatcttat atatgcaaac cgatcggagt
       61 atctcatgaa ctaatcggct gtattgaacg tacatatatc aacacggctg tcgtgtatgt
      121 tgaaagttat gaccaacgag actatcaact tatcaatgaa agcaagtatc gcatgctcgt
      181 aaaattctgt gatattagcg cacctactga attggtttct taaacagtat ttttgagaat
      241 tcgcaagaac tcccttataa tataaataaa gacagaaggg agcgagaaga tatgccaaaa
      301 agaaaaagtt cagggaaaat gggaattatt cttgcagtga tctctgcaat tcctgctatt
      361 ttagctattg ttcgtgcgat gaaggaatca agagaaagtt aagcacacat tatgagcgtc
      421 aaacaaaaaa cgcatatacc tgcggtggca ggttatgcgt tttttgttgt ataaagactt
      481 ttttcattaa cgatttttga ttagccgact cccaaaacaa aatttagcag tttatctgaa
      541 taagtgattt cttcatctgt ttgtttcaca ctttcattta gtagtcccat ctcatcggtt
      601 atgataccat ccacaccata aaacatcatg cgactcatgg catctgaatc gttgattgtc
      661 catacatata cttcttttcc gtcttggtgg gcagaattaa tgaagttacg gttgatcgta
      721 ctatattcca ttgtcagaaa atcagcaggc gtcacagggg gcccaacaat attgaaaggt
      781 aagatgtagc ccacataaaa gtttggttct gcttctttta aatctgaaac ggtttggtac
      841 gtcaaacttt gcaaaatatg atggttctct aagataactt tgcggtattt atggacaaat
      901 cgttcgacaa ggtcatccga atctttttta gaggtcttga tctcaataag taatttttgg
      961 tttagcttat gtgctttttc caaataagaa tcaaaagaac aaacaggggc ttgcactccg
     1021 ttttcagtaa ctgtaagatt tgtcaattct ttcaatgtaa gatcctgtgg tgttccattt
     1081 acccctgtta agtgacgaag atttgcatca tgcatcacta cgaaatgacc atcttttgtt
     1141 tcctgaacat ccatctctac ataatcagga tgatagtctc tgcttgtttt ttcaagtgct
     1201 tctaaagtat tttgcgcccc attttgggta ctcacacctc tgtgagagac agtcactggt
     1261 tttgtctgaa cagctgaagt cagataatcc atattgtaaa gacagacgcc aataccgaaa
     1321 aaaactgcaa aaagagtcaa accagtgttt ttcagcgcag agaaacgcaa gttgggggct
     1381 tgtgggataa accacttagg gatctcaggc aagaagcctt cgtcatccat gaaatcaaca
     1441 atgatataaa aaatccctac ggtagacatc acgatattga ataataaaat cccttgtaaa
     1501 agcgtcatag caaacacagc gctgatcaat gcatagtctg ggaaaaattg ttcaaccata
     1561 ctttgtgcca aaataacaac gatatatcct aaagaagaaa ggagtaaaat cgtaccgcca
     1621 atcacaataa attggcccgt gatggccaaa agacgcgatt tggtcaatga ccagctttcc
     1681 cgaatggctg ctcgaaaaga acgatctcgt agaatcattt ccggtaaagc aaaaatcagt
     1741 cgaatcccaa tatagccaag gaaaatataa acgagtaaaa aggaactgac gatgatccaa
     1801 cgattagcaa aaataaaatc cataatgaaa gcggggattt ttatttttga cagtagatca
     1861 gaattaaaac ttaatccgct gatcggtaga atcaataaaa aataagcaag gaaaaacaaa
     1921 aaagtaatgg gtctcacttt cttcagctga agtatcgtca agtgtaagag ttgcttcaaa
     1981 gagattggtt ctttcttttt aataaaaaaa acactcatca aaagaaaagt aaattcgaaa
     2041 taaactaaca gtaaaatcgt catcaaaact acgattaacg agagtagaac cccaggatgt
     2101 tgagaaaaaa ttgaaggaat attgtcataa gacaaatagt caattgcccc tcggtgtaaa
     2161 ataaaacggg tcgtactgct caataagggt aaacaaataa ataaaataaa gccatgcatc
     2221 agcaagacat cacggaaata agcttgtgtt ccttttaaaa agtcccaagc attacctaag
     2281 ctacgtttca aataattcat cctatcacct gctcttcaca ttgtagcgca aatctaatga
     2341 aaaaggaaag ggtgcagtgt gttttgcaga ccctttccca gatttaa
//
LOCUS       DAZUKD010000204         2364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000204 DAZUKD010000000
VERSION     DAZUKD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.212"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..470
                     /locus_tag="ACG8N9_002854"
     CDS             <1..470
                     /locus_tag="ACG8N9_002854"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4898380.1"
                     /translation="KELVNVYREHKPDINFLENADTLLTELRSRGYKLGIITDGYAIT
                     QRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLEKFNLKAQECIYIGDNELK
                     DFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIRSLWEIKNFLGVIS"
     gene            467..1585
                     /locus_tag="ACG8N9_002855"
     CDS             467..1585
                     /locus_tag="ACG8N9_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFS4898381.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1575..2195
                     /locus_tag="ACG8N9_002856"
     CDS             1575..2195
                     /locus_tag="ACG8N9_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4898382.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT          897 a          300 c          456 g          711 t
ORIGIN      
        1 ttaaagaact ggtaaatgta tatcgagaac acaaaccaga cataaatttt ttagaaaatg
       61 cagatacttt attaacggaa ttaagaagta gaggatacaa gctggggatt attacagatg
      121 ggtatgctat aacccaacga aacaaattgg atgctttgaa agtagactat ttggtagatt
      181 atatttgtat tactgacgaa ttaggccgtg aattgtggaa accacaccct atctcatatc
      241 ttttattgtt agaaaagttt aatttaaagg cacaagagtg tatatatatt ggcgataatg
      301 agttgaaaga ttttataact gccaaatcaa taggaatgca aacaattaaa atagaaagag
      361 taaatggtat ttacagagaa agcagtttga agttcaaaag attttaccaa gctgataaaa
      421 aaatacgttc gttatgggag ataaaaaatt ttttgggagt gatttcatga aaagtaaaag
      481 aattctatta gcttctccac atatgagtga agaaggatat gaaaaagagt atgtaaaaga
      541 agcatttgat acaaattggg ttgctccttt aggtgcgaat gtgaatggat ttgagaaaga
      601 attagcagaa tacgtgggag ctagtcatgc tgccgcatta tcttctggaa cagcagctat
      661 acatttagct ttgaaagcgg ctggggtcgg taaagatgac atagtgattt gtcagtcctt
      721 gacgttctca gctactgcta atccaattct ctatcaagga gctatacctg tatttgttga
      781 tagtgattac gaaacttgga atatgaaacc tgaacatttg cgaacagcac tagaaaaata
      841 tcctaacaca aaggctgtat tagtagtaca tttgtacggt ttatcagcta atttagacga
      901 aattactaaa atatgcaaag aatacgatgt tactctaatt gaagatgctg cagaatcatt
      961 aggaacaaca tataaaggaa aatatactgg aacatttggc gactatggaa tttattcttt
     1021 taatggaaat aagataatca caacttcagg tggcggaatg cttgtatcca atgacaaaga
     1081 aaaggtggaa aaagtgagat tttgggctac gcaatctcgt gagaaggcac gccattatca
     1141 acacaatgaa attggctata attatcgaat gagcaatatc gttgctggta tcggtcgagg
     1201 acaattgaaa gtattaaatg aaagaattga gaaaaaacga tatattcata gtttttataa
     1261 aaaagaattg tctaaattgt caaatataca attcatgcca gaaaataatt ggaatgaacc
     1321 gaattattgg ttaagtgtca taacaataaa gggaataaat gcgaatcatc ttattgatga
     1381 gttagacaaa aataatatag aagcccgccc agtatggaaa cctatgcatt tacaacctgt
     1441 ttttaaaaat tatgattata ttggcgatga tgtagcagaa actttatttg acttaggagt
     1501 atgtttacca tcagatacaa agatgacaga tgaagattta aaatatgtag tatctacgat
     1561 taaaagagtg atcaatgatg cgtaaaaaaa ctttctatca gctttttggg aaaagaattt
     1621 tagatatcct attgtcagga attgctctca tcgttttatc gccgataatc ttaattgtag
     1681 gttttttagt aagaattaaa cttggttctc caattatctt caaacaagaa cgaccaggaa
     1741 aaagtgaaaa aattttttcc atgtataaat ttcgtacaat gactgacgaa agagaccaca
     1801 atggagaata tttacctgat gaaattcgac taactaaatt tgggaaaatg cttagagcaa
     1861 caagtctgga tgagctaccc gaactttgga atattttgaa aggtgatatg tcgatcgtag
     1921 gacctcgtcc attattagtg gagtatttgc cactatatag tgagaagcaa cggaaaagac
     1981 acaacgtaag accaggatta actggactgg ctcaggtcaa tggaagaaat gctattagtt
     2041 gggaagaaaa gtttgattta gatgtttatt atgttgataa aattagtttc tttaatgatc
     2101 ttattattat aattcagaca tgtaaaaaag taatcaagaa agagaatatt aatacaatca
     2161 ataacaatat cccagaaaaa tttaaaggga gttaaataca gcagcttaat gcaagttttg
     2221 tgacttgcat tatatttagg tataaatcaa agcgagtata ttagaattat ataacatatg
     2281 ttaaaaaaac gtatagctaa taaaagtatg attttaaaaa tttgtgtaga gttttaaata
     2341 tactgtttca atacgagaaa atta
//
LOCUS       DAZUKD010000205         2346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000205 DAZUKD010000000
VERSION     DAZUKD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.213"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..2203
                     /locus_tag="ACG8N9_002857"
     CDS             50..2203
                     /locus_tag="ACG8N9_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4898383.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          802 a          416 c          527 g          601 t
ORIGIN      
        1 gtttttgggt acaatgaagc atgaatgtaa ataggcggaa ggataggaaa tgatgaaaga
       61 ttttatgaaa aaaatattaa atacttcttg gttagaaaaa ataagaaaag gtgcacaaga
      121 acctaagaac aaaacgtttc gccggtccca tgttcctttt cgcttaaatt ttcttttttt
      181 cattatattt acgttatttg ttgcgttgat tgctagattg ggttatttgc agatcgtgaa
      241 cggcgaagag atggaggcgc gagtgaagtc tacttctacg attacggtcc aagaaagtac
      301 tcctcgcgga atgatctacg actccacagg aaaagcttta gttacgaata aagcgaatca
      361 ggccatcaca tatacgagag gaacacaagt tacggctaaa gatttgcttg aaatcgcaac
      421 gaagctgaac tcgctgatcg atgttccggt agatgaaaat ctgacagata gagataaaaa
      481 agatttttgg ctggctgatc ccaaacattt gaaagaagcc acaaaaagac tttcggcaaa
      541 agaaaagcaa ttagggaaca gcgaacaata tagtgcgatg gtcaataaag tcacagctga
      601 tgagattaat ttaaacgagg agcaattaaa aattgctacc atctttaaac ggatgaatag
      661 tgcatatgaa ttaaacactg tctatatcaa aaactctggt gttactgatc aagagttagc
      721 tgttgtggca gaacgcgctt ctgaacttcc aggagtttcg actggcacag actggacacg
      781 agaatataat gcagcaagtt cactgaaaag tattcttggc agtgtgacga ctgaaaagca
      841 aggacttcca gcagatgaag cagagaaata cctagcaaaa ggttattctc ggaatgaccg
      901 tgtaggacaa agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa ccaaaaccca
      961 atatgaagtt tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa tcttctctgg
     1021 ggaaaaaggt tctaatttaa tgttatctat gaatgcagaa ttccaatcaa aagtagaaga
     1081 aattttgaaa aggaattatc aaacattgat caacaacggc aaagcacagt attcgcctgg
     1141 tgcttatgca gtggctatga atcctcagac aggagaagtg ctagcaatga ctggtttcag
     1201 tcatgaacaa ggttccaaag aaatcaccga gaatgcttta ggcacgatta ctagcgcctt
     1261 tgatccaggg tctgtagtca aggctggtac tttgacagca ggctgggcaa gtaacgcgat
     1321 cagcggaaat caagttttga ttgatgagcc gattcgtctg caaggtgctt cagaaaaaag
     1381 ctctgtcttt aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat cggaattgtc
     1441 ttctaatacg tatatgatca aagtggcgtt aaagatgtta ggactagact atacacctgg
     1501 aatgggacta cctagtttag atgaagaagc taaagcttat cagcaattga gagactcttt
     1561 caaagagttt ggtcttggta caacgactgg gatcgatttg ccgaatgaat ctcctgggat
     1621 ctcacgttct gttgattata tgaagaagtt caatgcggat aatggcaaag aatggtatgc
     1681 tccaggtaac tttaccgatt tggcatttgg acagtttgac acatatacac caatccaatt
     1741 agctcaatat gcttcaacag tcgctaatgg cgggaaacga gtacagccgc acctagtcaa
     1801 agcgatctat ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag aaattggcac
     1861 aacagtagaa aatacggtca atatttctgc agaaaatatg gcgatcctcc gtgaaggatt
     1921 ccaccaagtt gtccatggta ccgatcctta cacaacagcc aaaccattag ccagtgcgaa
     1981 aatggacctt tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc atccagatat
     2041 tgcgacagtc aatagtaata tcgtggcata cggaccaaca gacaatccgg aaattgccat
     2101 cagtgttgtc ttgccgaact tattagacga acaagaccac ataaacttga cgattgctaa
     2161 agaaataatg gacgcgtatt atgatatgtt catggcaaaa taaaaaagag gtcgtgaaaa
     2221 agccgcttag tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca catttttgct
     2281 taaatttgac ttcataccga gaaactggct tttgaacacc gtttactcgg caaaaaaggt
     2341 tgtgac
//
LOCUS       DAZUKD010000206         2317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000206 DAZUKD010000000
VERSION     DAZUKD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.214"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG8N9_002858"
     CDS             <1..350
                     /locus_tag="ACG8N9_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4898384.1"
                     /translation="EAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFE
                     DAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDE
                     WIYSSRKYINFDK"
     gene            407..799
                     /locus_tag="ACG8N9_002859"
     CDS             407..799
                     /locus_tag="ACG8N9_002859"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898385.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            800..2239
                     /locus_tag="ACG8N9_002860"
                     /note="aph(2'')-Ia"
     CDS             800..2239
                     /locus_tag="ACG8N9_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4898386.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRAYKD"
BASE COUNT          951 a          209 c          400 g          757 t
ORIGIN      
        1 gagaagctgt taaaggaatt tttaagttca cagatattaa cttagcgcgt gaggctaaaa
       61 atcgattgat tcatgattat atcgatcaac caaaatattc aaaagcttgc gcatcattgg
      121 atgatggatt cgaagacgcc tttcaatata ccgtacaagg aaattcccac aatcgactaa
      181 agagtaccaa tctaattgaa cgactgaatc aagaagtacg cagaagagaa aagattattc
      241 gcatcttccc caatcaaaca tcagccaatc gcttaattgg agccgttctt atggacctac
      301 atgatgaatg gatttattct tcaagaaaat acatcaattt tgataagtag aaatggtaaa
      361 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
      421 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      481 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      541 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      601 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      661 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      721 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      781 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      841 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      901 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      961 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
     1021 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
     1081 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
     1141 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
     1201 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
     1261 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
     1321 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1381 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1441 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1501 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1561 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1621 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1681 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1741 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1801 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1861 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1921 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1981 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     2041 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     2101 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     2161 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     2221 aagggcttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     2281 taaatagttt aaaaaaatga aaaataataa aggaagt
//
LOCUS       DAZUKD010000207         2283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000207 DAZUKD010000000
VERSION     DAZUKD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.215"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8N9_002861"
     CDS             complement(<1..300)
                     /locus_tag="ACG8N9_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4898387.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8N9_002862"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8N9_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8N9_002863"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8N9_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898388.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="ACG8N9_002864"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="ACG8N9_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          652 a          433 c          333 g          865 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tac
//
LOCUS       DAZUKD010000208         2260 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000208 DAZUKD010000000
VERSION     DAZUKD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.216"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACG8N9_002865"
     CDS             complement(365..1657)
                     /locus_tag="ACG8N9_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4898389.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACG8N9_002866"
     CDS             complement(1931..2191)
                     /locus_tag="ACG8N9_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898390.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          746 a          430 c          343 g          741 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa
//
LOCUS       DAZUKD010000209         2228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000209 DAZUKD010000000
VERSION     DAZUKD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.217"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG8N9_002867"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG8N9_002867"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4898391.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8N9_002868"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8N9_002868"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4898392.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT          807 a          356 c          471 g          594 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtat
//
LOCUS       DAZUKD010000210         2186 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000210 DAZUKD010000000
VERSION     DAZUKD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.219"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1249)
                     /locus_tag="ACG8N9_002869"
     CDS             complement(<1..1249)
                     /locus_tag="ACG8N9_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4898393.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILI"
     gene            complement(1398..2111)
                     /locus_tag="ACG8N9_002870"
     CDS             complement(1398..2111)
                     /locus_tag="ACG8N9_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4898394.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT          741 a          417 c          307 g          721 t
ORIGIN      
        1 taattaatat ttgtaaaaca gctgctcgcc agtctccggc tgttgctaaa aatcccgata
       61 taactggtgg cgtagtccaa ggaatttgca ctactacatg attaattaat cccacagcag
      121 ttgcaaaata tgctactgtc aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac
      181 tgatattaaa tacaataggc aatccaaaaa taataggttc gcttatattg aatatagcgg
      241 gagttaccga taatttagca aagtccttat aatcttttct acgactgaag ataaatattg
      301 caatcaataa agcaatcgta tttcccgatc cacccattac cgcaaaggta tctttaaagg
      361 ccatattgat aatatgtgga atttgctctt tgtttgcata agctgccata ttttcttgca
      421 tattctgaat caaaaacgga tctaacaatg aacctgatat tactgcttga tggataccaa
      481 atccaaaaaa tagattagca atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa
      541 ttttacttaa tggctctgta actatcgtgg taattagctt atttatatcc atattaaaca
      601 attgaatgat tataaatgac aataaagcaa acgaggtaat cgtaataatg attgggatta
      661 atacattaaa agactttaaa acagcgggag gaatatttcc ggatagctga atctgtagtt
      721 tcttattatt agaaagttta ataaataatt ctgtagccgc taatccaaca aatataccta
      781 caaacattcc tgaagctcct gtataggata taggtatcac acccgtcact tcaacagctt
      841 tatccgcatt ttcaggagta aacatcatct gcataggtgt taatacaaca aatgtcgaaa
      901 tagcaactaa taccgctgca atatgatttg tataatttct gcttgcacac atatgataag
      961 atactgctgc tacaactaat aatgtagtta tgcttaatgt accattggca attgaagtac
     1021 caatttgttg taacgtggtc aatgtactag tatcgacaat ttcccccatg aatcctgttg
     1081 gttctaagat cactgaatta ataagtgtca caaatcctgc taacatcata aatggcataa
     1141 atgtagcaaa cgcatctcgc aaagttctta aatggatctg attccccaac ttagtcgcaa
     1201 ctataccaaa tttttcaaat acaatgtcct tcctgctact tttgcccata ttttttctcc
     1261 tttatgctta ttgtaatcga ttactttttc cacccaaata atacatcact tatcaaaaaa
     1321 agtaaatact tttaattaaa tatatatatc ttttttagta taaaaaaata agctctgaaa
     1381 aaataatttt cccgagctta tttttcaaca aaaacagctt caaaaacaaa gtcttgatat
     1441 aaatgtctcg ataccgaata ctcgaaaggt ttaccagacg atagagcaac aactttgtct
     1501 atctcaatca taaattcacc tggatctatt tttaatattt ttgcgtcaaa ttcattcgca
     1561 gtatctcctt tgatacgtat gtgcgaagtc tttatttcca gacccagttc atttgtgata
     1621 taagcatata cagatttctt taaatgtttt ggttccaagc ctggtatgac tgctaatggc
     1681 atgaatgttt gctcaatcga gtatggttga caatcaataa aacggcttcg tacaatatta
     1741 tatacatagt cttttacact gatacttaat ttatcagcta cttttttcga tggaagttca
     1801 attgagaaag tatggatttc tgaatctacg tgttcttcac caaccacgct aaacaaacct
     1861 tcgtttggac caagaactcg tgagttatca attggtgttc tgacgacttc agtcccatac
     1921 ccacttctac ttcgcaaaac tccttcggaa acaagtaagt ctacgccttt ctttactgtt
     1981 aacttgcttg tatgataaat aactgccaat tcatctcctg taggaagttt ctcaccaatt
     2041 ttatactctc catttacaat ttttttcttt atatctaagt aaatttccat atacttcgca
     2101 atttttttca atggtgaccc tccaatcaaa aacatatctt catctatact ttaacataaa
     2161 taaaccgata aaatccacat caaaat
//
LOCUS       DAZUKD010000211         2157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000211 DAZUKD010000000
VERSION     DAZUKD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.220"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8N9_002871"
     CDS             complement(<1..914)
                     /locus_tag="ACG8N9_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4898395.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8N9_002872"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8N9_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4898396.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          732 a          381 c          416 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaa
//
LOCUS       DAZUKD010000212         2136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000212 DAZUKD010000000
VERSION     DAZUKD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.221"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..>2136
                     /gene="acm"
                     /locus_tag="ACG8N9_002873"
     CDS             31..>2136
                     /gene="acm"
                     /locus_tag="ACG8N9_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4898397.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVII"
BASE COUNT          843 a          343 c          414 g          536 t
ORIGIN      
        1 tttttagtta aaatagggga gatagaaaaa atgaaaaagt gtagaaatat tattttatca
       61 atgttattta ttattactaa tataacatca ctgattcctg ttcatgtcta tgcggatgca
      121 ggcagagata tcagcagtaa tgtcacttcg ttgaccgtgg acccaacgaa tattacagat
      181 ggaggaaaca taaaagtaaa attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt
      241 gattatcttt ggatcaattg gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa
      301 gaaatcccat taatgattga aaataaaaat gttggaactt taacggtcag aaaagattct
      361 gcgcaggttg tgtttaatga aaatattaaa aacctagact cagtagaagg ttgggggcaa
      421 tttgagatac aggccagaaa cgtaaccgat accggtgaag aaaatacggg accattcacc
      481 gtgacgagcg gtgataaaac agctactgta aatgtgacaa agccagcttc tggttcttct
      541 tctagcgttt tttattataa aacaggagac atgctacctg aagatactaa gcacattcga
      601 tggtttttga atattaacaa taacggcact tatgttgaac aaccagttaa aatcagtgat
      661 gaaatccaaa gtggacaaag attagatcca agtacgtttg aaattaatca aatacactta
      721 ggtgaacaga aggtttatag aggagaagag ggaatccaac aatttttaca agatttcccc
      781 agcgctacat ttaatttttc agtaactgat aattacattg aaataactat accaaaaaac
      841 tttgtcaatc ttaggaaaat aatggtgagt tataagacga tcatagaaaa cccagaacaa
      901 ataaattttg agaatcattc ggaagcttgg tttaaagaat ttaataaacc tgctgttgat
      961 ggagaaagtt tcaatcatac agttaaaaat atttcagctt ctggtggagt caacggtaca
     1021 gttagaggcg aattgaagat tttcaaatat atcaatgaca cagagattgg tatcccaaat
     1081 gtgactttcg aattaagacg agcagatgaa cagcctattc aaggacagtc tagtatcctg
     1141 ttgacttcga atgaacaggg agaagctagt atcaaaggcc tgcaagtagg agactatgtt
     1201 gtaaaagaaa aagaagcacc caattggatt gattttgatc cactaagttc aaatgaactc
     1261 aaattctcca taaacgaaaa tgatacagag ggagtcagtt tacctattta taacaagaaa
     1321 aaggttacga acatcacagc aactaaaatt tggaacggtg gaaccactcc aagaccgagt
     1381 atttatttta aattattcag agcaagtaca aacaattggg aaccagttcc tgatgcagaa
     1441 acaaaacgat tagacaatgg aataacttca gtgacttggg aagatatcca acagtatgat
     1501 gatagtggaa acgaatatac gtttaaagtg caagaagtag atcaaaacgg aaatgattat
     1561 gtcccttcag gttatcaaaa aattgaaaac gggctagtcg ttacaaatga gaataaagaa
     1621 gtcatttcag tctcaggaca aaaaacatgg gaagacaatg agaaccaaga cggcaaacga
     1681 ccgacgacga tcacagtaaa tctattagcc gacggtcaac cgatccaaca caaagaagta
     1741 agtgaaaaag atgattggcg ctatcgtttc acaaatctgc caaaatacag agacgggcaa
     1801 gaaatcatct atactgtaac ggaagacaac gtgcccgaat acagtacgac gattgacggg
     1861 tataacatca aaaacagtta tacaccggaa aaaacaaata tatctatacc aggtagccat
     1921 ataacaccgt ggaaaaattt tccagagaag acagagagag agaaaattag aaatctattc
     1981 tctcaaaaat tgcaaatatt tgctcataca ggagaatcca gcgaacaaag aagcaattat
     2041 attagtaaga gagctgtacc taaacagata acaaataaat caaaaagcat tcttcctaaa
     2101 acaagtagta aaaaatctag ttttgctgta atcatt
//
LOCUS       DAZUKD010000213         2131 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000213 DAZUKD010000000
VERSION     DAZUKD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.222"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..1600)
                     /locus_tag="ACG8N9_002874"
     CDS             complement(53..1600)
                     /locus_tag="ACG8N9_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4898398.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1702..2055)
                     /gene="tnpB"
                     /locus_tag="ACG8N9_002875"
     CDS             complement(1702..2055)
                     /gene="tnpB"
                     /locus_tag="ACG8N9_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4898399.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          607 a          441 c          379 g          704 t
ORIGIN      
        1 tgtacgtcaa ataacccgac ctatagaata gaatcgggaa tgtttggcgt ttttagttac
       61 gagaaaaatg ttgttgtaca tccttgttcc aaggcaacag ttgatctaaa gtttctgctg
      121 aaagtacggg aagatttggt atcttatcaa atagaaactc tagatacttt acaggattca
      181 atccgttaag ttctgctgtt ttatatacac tcagaatgat tcctgaagtt cgtgctcctt
      241 caagacttgt ggaatgcaac caatttttcc gtcctatcac cagctctttg atatttcgct
      301 ccgcacgatt atttgtgagt tctaaacgac catcttcaaa aataggttca aaaccacttc
      361 gtagtttaca cgcatattga acggctgcat ccaacttgga ttttgcaaca ggatctatcg
      421 ttgccatcca ctcataaaaa gcaataaata atggacgcag ttcttgttgc cgacagctta
      481 atcgcttctc aggagaaaag tctttccatt tcttttctag gacaaaccat ttatccaatt
      541 gtttgactgc ttgagctgcg ggaatatccg tgtttttctt tccttttgga attgcttcga
      601 aaaatttccg ccgaacatgc gcgaaacaat agacaagtcg tacactttcc acggcattat
      661 acccgctata gccgtcacag tgtaaatacc ctgtaaactc cttgagaaaa gttttcggaa
      721 catctccagc acgactttcg tcgtggtgat aataaacgat gggatgctga ctgtcttttc
      781 ctgtacaaaa ctgccagtaa taggtatctg ttttggcact ttcaatcaca cgataacttg
      841 tttcatcccc gtgtagaatc tcttcttgat taagatagac tcttaatcgt tctcctaaag
      901 ggtctaacgc gttttgcact gctttaatat gccaattggt gatattatct ctcgaaatat
      961 ccaacccatg tgtttgtttc caatagttct cttgccgata ggcaggaact ttctgaccaa
     1021 atttcatccg gatcgtttcc gccactaaac tattggaacc taagctatta gaaatcaaag
     1081 gtttaggaac gatcgctttt ttaaaagaag aaagtccttc tttttcgcag cttttacaac
     1141 aataggtatg ttgataatgg gtgagacaat ccaacatagc ctgatggaaa cgaatttctt
     1201 ctcgtacttt tgtccgtccg atatctttca tctgtgaacc gcaatgttca caaaattgtt
     1261 cttccaaagg aaggagatgt tctttctctg ttataggtaa gtgtttgatt ttgtctgctt
     1321 tcgttcctac aactcgttga tgtcctttta ctggaataat ggtggcagct gcttctggaa
     1381 cacttacaga aatttcttca tcactaaaaa gattcaattg cccattcgtt tcctcaaaga
     1441 gagtctcttt agagcgtcca aataatttgt tggttaaata attgaccttt tcattcaata
     1501 gttggatttc agttgtaaga ttctgaatgg tttgttgttg tgcttgattg gttttatggg
     1561 cttcttgaag caactgaagc aacgtatctg tagtttccaa aatttttcac caccttgaat
     1621 cgaactgatt ttgttacaga ataccacaag aaacgcacaa gaaatagggg ttcaacacat
     1681 tttttgtgtt aaccccccag cttaaaaaac tccttttttt cctggcaaaa tagctcgagg
     1741 ttgatcgata cttaatcctt ctaacaacca tcgaacctgt tggttcgtta aggttcgaac
     1801 ttcgtctttc gtccgaggcc attttaactt gccgttttct atccgcttat aacagagaag
     1861 aaaaccatca ccgtcccaat agagaagttt gaaacggtct tgacggttcc cgcaaaataa
     1921 aaaaacggca tcttcgtaaa gctcgagttg atactcttgt tgaataagac tggcaagacc
     1981 atcaattcct tttcgcaaat ctgtttttcc gcacacaata taaatatgtt tggtttgaag
     2041 taaatcataa agcattggac aacagttctt tcaaaatagc ttgaagaata taattgttaa
     2101 taccgttata gatcaaaact tcagaaccat t
//
LOCUS       DAZUKD010000214         2078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000214 DAZUKD010000000
VERSION     DAZUKD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.223"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8N9_002876"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8N9_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4898400.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8N9_002877"
     CDS             852..1040
                     /locus_tag="ACG8N9_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4898401.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8N9_002878"
     CDS             1053..1610
                     /locus_tag="ACG8N9_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4898402.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8N9_002879"
     CDS             1642..1884
                     /locus_tag="ACG8N9_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4898403.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          763 a          315 c          415 g          585 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttattt
//
LOCUS       DAZUKD010000215         2045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000215 DAZUKD010000000
VERSION     DAZUKD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.224"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG8N9_002880"
     CDS             25..696
                     /locus_tag="ACG8N9_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4898404.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG8N9_002881"
     CDS             755..1195
                     /locus_tag="ACG8N9_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898405.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG8N9_002882"
     CDS             1207..1875
                     /locus_tag="ACG8N9_002882"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4898406.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..>2045
                     /locus_tag="ACG8N9_002883"
     CDS             1939..>2045
                     /locus_tag="ACG8N9_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFS4898407.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMD"
BASE COUNT          736 a          325 c          425 g          559 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaa
//
LOCUS       DAZUKD010000216         2020 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000216 DAZUKD010000000
VERSION     DAZUKD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.225"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..571
                     /locus_tag="ACG8N9_002884"
     CDS             101..571
                     /locus_tag="ACG8N9_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4898408.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="ACG8N9_002885"
     CDS             793..1896
                     /locus_tag="ACG8N9_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4898409.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          328 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac
//
LOCUS       DAZUKD010000217         2007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000217 DAZUKD010000000
VERSION     DAZUKD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.226"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..501)
                     /locus_tag="ACG8N9_002886"
                     /pseudo
     CDS             complement(76..501)
                     /locus_tag="ACG8N9_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(610..1641)
                     /locus_tag="ACG8N9_002887"
     CDS             complement(610..1641)
                     /locus_tag="ACG8N9_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4898410.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          615 a          335 c          346 g          711 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggttaggaa tctgttttga ttttgttcca tttgaaattt ctctcactcg
      121 cttttttgtc tcatcatcaa ataaaatgaa agggtcttct aaacgatcga taaatacttc
      181 cattgttgtt gttggtagat gataagaagt catgacaggc actgcaccaa ggtaagacac
      241 agaaacagct agaaggtatg tgtcaaaagc ggaactctta taaataataa ctttgtctcc
      301 ggatttcact ccaagatgag ccaattgata agcccgtttc aacacacttt cgcagcattt
      361 tttgtaagtt gtttcaagtc ctaattctgg gaaagctgaa agtggctcat caaagataat
      421 cggggtctcg gaaaagttag ctgcagcttc ttgaaaattc gtaaataagt tcaaagggga
      481 atacgttatg gtctccatca ttcacactcc attatctttt gttaagtaat atacaaattc
      541 aagataatcg taacataatc ctttatataa taacatagtt tattgtgaaa aaaagatcat
      601 acatttgttt tatatggaag aaacccgaac gaatgccgct aattcgtgat aagaaaaatc
      661 aaccatcttt tcccagatat cttgaaaaga atgggatgag aggtatcgat taccacataa
      721 ataatttacc gcgtcagcat catctaaata tagttctgca taataacgat atgtttttct
      781 gcatagttct ggcgatacgt ttttatatac tgtaattttg ctcaaaattt ggcgaatagt
      841 ccgaggagaa atttgctgat catgtgagga acgaaaaaca aaagaatttt cgtccgtatt
      901 ttgtgctgtt aacttaaaat agttctccag aaatggcatc gcttcttttg gtattggtac
      961 tttattcagt ctgttttctt ttctattcag attaagaatc tgcttggtca aagatacatc
     1021 tttacgttta agttctacta cttcatggat ctttaatccg caatacagaa gaatggcagt
     1081 aattgcaagg ttcctcggtc tattcttttc ttcaaagcta gcttctctgc ttgtttgaaa
     1141 gccttcaatc gggataaaca aatgctcaaa atctttgaaa agttctgcat cgatatatcg
     1201 cggagctgct ttggttgttt ccggtaaact tgtacccaaa agagaaatct cttcttcaat
     1261 cagtcctttt tgatacaaaa atcgtaaaaa atgatttaat gaatattgct tccttgaaat
     1321 cccgtaatag ctattttcaa acaattgctc cgtttcttgg ttcgctgtag tttggaacga
     1381 acgagtatgt cgatagattt tagctaagaa attctgataa tcttgttttt ctaaatggat
     1441 aatcagctga tcatccactt cttcagaaaa tacttttctc aaataatcag taaacagatg
     1501 aaaatctttt gcgtattcat agcgactgcc taatgaaagg gaagaacgat tttcttctaa
     1561 aaactgttga aagacggaag ggagcaagtg tgtacgttgc tttaactttt cttgcagttg
     1621 ttttctagtt ttgctgtcca ttacaaattc cctccaatag tgttcatttt ctatatttta
     1681 ccgcatttca tcaaatgaag atagacagtt tgtcttgatc ttaggaagca aatttttaac
     1741 taagtactag ctaaaagata tgaagtaaga agatacattt ttagttattt tattagtgca
     1801 gtcattcttt tgatctttta attgatttat cctcttttta gctatgtggt tacttcatga
     1861 gcaatgaaat ggatttggtt cgttctaatg gcaggaggct ctttctttta tttccgaaaa
     1921 attagaaaac attttttcag aatcgattgg ctctccattt attcaataat ggtttgtcta
     1981 acgatattgt tgggataatt cttaaag
//
LOCUS       DAZUKD010000218         2002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000218 DAZUKD010000000
VERSION     DAZUKD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.227"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACG8N9_002888"
     CDS             <1..377
                     /locus_tag="ACG8N9_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4898411.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            425..1825
                     /locus_tag="ACG8N9_002889"
     CDS             425..1825
                     /locus_tag="ACG8N9_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4898412.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaatgtttc cacagaggac ttctttaatt attgtcgatt agaggaaagt ggtgtaacag
       61 tcactagcgg tacagattta ccgctgttta ttactgatat tcctgaatcg attataagag
      121 cagtattccg tcgttttcct gaaggagaag aagtctggga gaaagaaaca agcttcacga
      181 caaccgagct gctgaaaagt tttacaagca acagcttcaa agtaaatggt ttatcggagt
      241 acggagaaat aaaagtaggg aaaagcgcca gcttagctgt tttcagcgag gacctctgta
      301 ctgatgactg gcaaaaagtg atggctgcgg aagttatcgc tacctatctt gatggtgact
      361 tagtttacaa gaaataatgc ttttcgatat aacgttataa aacgaattaa agagaggtta
      421 aaaaatggaa acaatgaaaa gaaaattcac cttcccacat agttatacat tgatttttat
      481 cttaattatt ttggctgcaa ttttaacttg gataattcct agtggtcagt ttgagacagt
      541 tgttgaaaac gtcgcaggta tggaaaaaac aacggttgtt ccgggaacct atcaccaaat
      601 cgataaggca gattatggac aaggtttttc ctctatacta atggcaccag ctgaagggat
      661 tatttcagct gttgaggttg ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat
      721 cctaaaaacg ggagccatcg atcgagggct gtttacctta gcaaaagttc tgggagacaa
      781 ggggatctta gttattccga tgtcgatgat tctattcagc ttaggaggat caacctttgg
      841 catgagcgag gaagtcattc ccttatttgc ggtgtttatt tctctgatgt tttctcttgg
      901 ctatgattcc atgactgcga tcttgattct atttttaagt acacaagcgg gatatgttgg
      961 gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg tttacggaaa
     1021 tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt caattgtttt
     1081 caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg tttatgagag
     1141 tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt tttcaacaca
     1201 agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt ggggtatcat
     1261 ttccaaggga tggtatatgg ttgaaatttc tgcactattc ttaggattag gtctttttgc
     1321 tgggggcatc gctagatttt ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc
     1381 agatttcgcc tatgctgctg taatcattgg gctagctaga ggagtcttgg taatcttgga
     1441 aggtggaatg attattgaca ctattctaaa cagcttggct aaccttttag gtggtcttcc
     1501 aaaattcctg ttcactacga ttctactcat ttctcaagta attgtttcct tttttgttcc
     1561 atcctcctca ggagctgcgg cattaacgat gccggttatg gcaccattgg gtgatctaat
     1621 gaatgtaagt cgtgatgttg ttgttctaag taaccagttt ggaaatggtt taatgaatat
     1681 tatctcccca actagtggtg tattactggc aggattagcc atcgcagaga ttaatttctc
     1741 taaatggttt aaggttggct tcaaaatatt tgccatctta acccttgttt ctgctgtttt
     1801 gttatacatt gctacactct tttagtctta atcatccatt aataggaggc aatagttatg
     1861 attaaaataa atcaattagc aggaatgaat atccactatt tattttattc tctcaaagaa
     1921 tttttcgctt atcaacagaa aattggtatt tcttctgttg aactttgggg aggtgctccg
     1981 cattttttca tagatgcttt ta
//
LOCUS       DAZUKD010000219         1989 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000219 DAZUKD010000000
VERSION     DAZUKD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.229"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            411..767
                     /locus_tag="ACG8N9_002890"
     CDS             411..767
                     /locus_tag="ACG8N9_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4898413.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            777..1676
                     /locus_tag="ACG8N9_002891"
     CDS             777..1676
                     /locus_tag="ACG8N9_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4898414.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          671 a          324 c          390 g          604 t
ORIGIN      
        1 ccctcgatat tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg
       61 attcctccaa agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc
      121 cgtgaacaaa aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta
      181 ttttttgtta tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt
      241 tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa
      301 tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt
      361 agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag
      421 aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt
      481 tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg
      541 atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt
      601 ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca
      661 tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac
      721 caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg
      781 gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc
      841 tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag
      901 atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat
      961 tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga
     1021 tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga
     1081 ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg
     1141 aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa
     1201 cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg
     1261 aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca
     1321 tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat
     1381 cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc
     1441 atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt
     1501 attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct
     1561 atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc
     1621 aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca
     1681 gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag
     1741 atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg
     1801 atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga
     1861 taatcacaaa aaaaaatata atatacattt tttatttaat tataattaat attgattgaa
     1921 gtctaggcag tcaagtcgag agtgtaaaat attttgtgta aatgaaaaaa tccatacaaa
     1981 aaaggaagt
//
LOCUS       DAZUKD010000220         1987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000220 DAZUKD010000000
VERSION     DAZUKD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.230"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..422)
                     /locus_tag="ACG8N9_002892"
     CDS             complement(<1..422)
                     /locus_tag="ACG8N9_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFS4898415.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKG"
     gene            complement(422..1264)
                     /locus_tag="ACG8N9_002893"
     CDS             complement(422..1264)
                     /locus_tag="ACG8N9_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS4898416.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(1266..1985)
                     /locus_tag="ACG8N9_002894"
     CDS             complement(1266..1985)
                     /locus_tag="ACG8N9_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4898417.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT          683 a          388 c          229 g          687 t
ORIGIN      
        1 ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa
       61 tagtctttgt tttcctcttt gttcgcattt ttaactaatt ttgtaaacca agcatttcct
      121 ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca
      181 tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa
      241 tcaattttat cttttgctat attttcggta atatctgcta aaatcgaatt agtaactact
      301 acttttaatt tttcgttcga agccacttta tctttgtctt tactatttat tgaacatgcc
      361 ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc
      421 atcataacga tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa
      481 ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat
      541 aaccaataaa caatccaagc aatgatgaac ttgctccgac tgttgcagaa ataaatatca
      601 tagttttcaa gctatttgca tataaatatg ctgtagctgc tggtgtaatt aacattgcaa
      661 caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaacca
      721 tcaatagata atgataaaaa ctgacattca tccctattgc ttttgccatt aatggatcaa
      781 aagaagtgat taataattct ttaaaaaata gaactactat aaccaacact aaaaccgaaa
      841 cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca aataaaatat
      901 gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctagt gctaaaaaag
      961 aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt tttataaaag
     1021 taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa ttaataccta
     1081 aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca cccataagag
     1141 acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct ataactacag
     1201 atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata aaattttgaa
     1261 gcatattatt ccccctctcc aataaatata gtatctccat aggctttttt taaattttct
     1321 tcattaaata cagattctac acttccgtga gcaattagat ttcgattcaa aagaataata
     1381 ttatcaaaat acttctttac cttgcttaaa tcatgatgca caattagcac tgtttttccc
     1441 tgatgtttca atttttttaa cgtatccata attattcgtt cactaactaa gtctatccct
     1501 acaaatggtt catctaaaaa tatgaaatcc gcattttgag caagacatct tgctaaaagt
     1561 acacgttgaa attgtcctcc agataattct cctatttgat gattagaata ttttcccata
     1621 tcgacctttt ccaaagcctt tgaaactctt tgccattctg catttttaat tctttgaaaa
     1681 actttcattc cggcatatgt tcccatagat acacattctt ttacttttat tggaaaagta
     1741 aaatcaatat cagctttttg ctctacatat gcaatcttca ccagtttttt attgatatct
     1801 tcattattaa tggagacctc tccctgatga gggataatat ttaacattgc tttaagcaat
     1861 gtggattttc cagcaccatt tggaccaata atacctgtta aagcaccttc ttgtattttt
     1921 aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa cattaagctt tttcacttct
     1981 aacataa
//
LOCUS       DAZUKD010000221         1979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000221 DAZUKD010000000
VERSION     DAZUKD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.231"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8N9_002895"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8N9_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8N9_002896"
     CDS             complement(728..1744)
                     /locus_tag="ACG8N9_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4898418.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          459 c          308 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZUKD010000222         1944 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000222 DAZUKD010000000
VERSION     DAZUKD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.232"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..>1944)
                     /gene="uidA"
                     /locus_tag="ACG8N9_002897"
     CDS             complement(155..>1944)
                     /gene="uidA"
                     /locus_tag="ACG8N9_002897"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4898419.1"
                     /translation="ETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESYPISVPSS
                     YNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGKFITQHK
                     GGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKENLVNFD
                     FFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILIAIYDEEA
                     QLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGIRSVQVTN
                     GKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEE
                     IMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKELIE
                     RDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDVC
                     KVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADTVA
                     GFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNKKG
                     IFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          615 a          396 c          235 g          698 t
ORIGIN      
        1 atacaacaat cagaaagccc ctttttttcg aaaaaactag aaaaaaaaca taaaaatatt
       61 tatcattctt taaacagaaa atctttctat aaaaaacaaa aaaaaacgat taggctaggc
      121 taatcgtttt ttgaacattc ttaaaaaatc caaattattt tttataactg aaattgggta
      181 ttgatgacca tcgtttctgt agatagtaag caatcatttt aggttttcta tctctagtaa
      241 agattccttt cttatttcct tgcactctta aaatcccttg acttgttgca aaatctgcaa
      301 aattccacac ttgttcacca acaaaatttt cacatgtatc tattacttcg tgatttgctt
      361 cgtaataagc cacttgatac tcttctgtaa acattgttgg tactgtatca tgaaatccag
      421 ctactgtatc tgctccatat tctgtaaaca ttattggcgt atcaggacat ctttccatcc
      481 atccatctag ctcttttttc aacagatatt ttgcatgtgc cagatcccct cccgaaacat
      541 accaaccata ataacgattt aaacataaaa catcgacaat atctcctacc ttacaaacat
      601 ctggcgttga ttttagataa gtaacgattg ttacaggtct tttctgcgga tcaagttctt
      661 tagttaattc aactaaaggt tcaaaatatt ctttagcacc ttcggaatcc gaatctggtt
      721 catttgcaat agaccacatt acaacacttg ggtggttctt gtcgcgttct atcaattcct
      781 tgatcaccgt tttatgagaa ttttctgttc gtatctcttt ccacgtatta tgtttttcca
      841 cattatcatc taataaagtg gccatgaaat ttaaatgtaa ccctactgca ggtacttcat
      901 caataactac aataccttct cgatctgcta atcgcataat ttcttctgag taaggataat
      961 gtgatgttct aaaagaatta gcacctatcc atttcattaa attaaaatct ttaatattta
     1021 cagcctcatt caaaccacgt ccattaacat acgaatcctc atgcttacca aatcctttaa
     1081 aataaaaagg tttattatta atcaaaaatt tgccattggt aacctgaaca ctgcgaatac
     1141 caaattcttc tgtataggta tcgatcactt tattatcatc aattaaacta attttcatac
     1201 tatataaata agcgtctaga ggttcccaaa aaattggatt cttaagctga atcattcctt
     1261 tctttccttc agcttcagct acaagttgag cctcttcgtc atatatcgct attaagattt
     1321 ttccggtcca ttctgtatcg atattgaatt ctacacttgc atccaaatca gttattcttg
     1381 tattaatagt aatatcactt atatagtctt tcggagttgt atataactta acaggtctat
     1441 gaatacctgc ataattgaag aaatcaaaat taactaagtt ttcctttttt atatccccat
     1501 tttcctgtac tgtttccttc ataatcccca caggtaaagt actatcgtca ataatattgt
     1561 ttacagcaac tgtaattcga ttattcccgt cttttacaaa ccgattaatt tcagactcaa
     1621 atggtgtaaa cccacctttg tgttgagtaa taaactttcc attcaaaaaa acttttgctt
     1681 catgtgttgc agatccaaaa cgaagaacta atctttcttc taataatctt ttaggaagag
     1741 tcacttcttt ttcataccac acccatccaa catgatttct aatgttctct gtttcaaata
     1801 aatcattata agaagaggga actgaaatcg gataactctc tttttcattt attggttcta
     1861 aagcaagttc ttcctgtaag tcatcatttg tatctaattt aaatctccag agaccgctta
     1921 agtcaattac ttgcctcgtc tctg
//
LOCUS       DAZUKD010000223         1848 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000223 DAZUKD010000000
VERSION     DAZUKD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.233"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1812
                     /locus_tag="ACG8N9_002898"
     CDS             <1..1812
                     /locus_tag="ACG8N9_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898420.1"
                     /translation="ESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKD
                     SWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVY
                     LGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGT
                     TGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALG
                     FEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLL
                     IFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVL
                     VAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLK
                     IKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKN
                     FEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEH
                     SFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDL
                     MMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          598 a          344 c          313 g          593 t
ORIGIN      
        1 gaatcttttt atttacgtat aaagtggagc ataatcatta tattatcatt gttattttta
       61 acaggaattg cttatttctt ttattttatt ttttctcctg cggacagcaa tcaaatgaaa
      121 gaaataaaag attcgtgggt tttctataca aaagatgatc ctgattttaa gtttgacagc
      181 aagtacactc gtacgattcc taccgtggat aaagacgaaa cattcattat ggaaacgaca
      241 cttgaaaaac cgttggacga agcaaattta ctaatcaaag gaaaccatca atggattact
      301 gtatatctag gcgaaaagat tttataccaa cggaaagatt atcaggaaga aagcaatccc
      361 ggactctcat tggctgtgat agatttacca gcaaattata tcggaaaaaa attgaccctt
      421 acagtctcat ctccttatca gaactatgct ggactgccac cgaaagttta tctaggaaca
      481 actggaccat tgattagttt tatttattca caatcagtgc cgcaagtatt gacgatgata
      541 atcgctgttt ttatcgctat agggaccttt ttctatacga tctacttgat gtataaacaa
      601 aaacgtcttg aatactcact tattctctta agttgctttg ctttggcatt aggatttgaa
      661 gctgtatcag aagatatttt aagtggtatt ctttttgaac cattcgttca ttctttttta
      721 tctcatttat ttattatctt aacgtcagct ttcatcatct gctactattg gagccgaatg
      781 ttttactata aaaaatggta cggaattttt tgtacgttcc aacttttgat ttttatcggc
      841 gttctgcttt atgcaacttt tactcctgca gaacttcctg aattaatgcc gattgctaat
      901 attgctagtg ttataagcac tttagtcact tcacttgcat gcattggcga agcatataag
      961 aataaccgtt tttatgtagc ttgtacacct tggatcgtct tggtagccat tatccattgc
     1021 ttgatttaca tccaaacggc attaggtatc tatcaaacaa cgatcaattg gtctacgatt
     1081 ttatatatta tcattctaat cgttattatc agctacaatc taatcgatca catcttaaat
     1141 accgacaaag atcggagaca agtagatttc ttaaaaatca aaaatgattt gttggagcaa
     1201 cattatgagc agcttcgaca gcatatccaa gaagtaaata cacttcgcct ggaatttgtc
     1261 caagagatgg agaatctgca agatttaatg catacggacc aaacaaaagc taaaaactat
     1321 gtggaaaaga tattagaaga agcaaaaaat ttcgaaatgc tgtgttcttt ttgcaaccac
     1381 caggtgacaa atcttatctt agcgcgttac cagaagttag ctgccaaacg gaatattcag
     1441 ataaccttcc aagcagatct tcccgaagaa cttcctatca acgatgacga tctggctcaa
     1501 cttttgatcc acgctttaga gcattccttt agagaaactc atgccatcga aaatccactg
     1561 tatcgaaaaa tttatttgtc gatacacgaa caagaggaac atttcgtcat ttgttgcgaa
     1621 catagtgcac attacgatac aaacctattt agtcgaggca tcacagaaga attagatgat
     1681 caggaacgtt ttgacttaat gatgatgaaa gacgtagcgg atcgttacac aggaacacta
     1741 acgcaggaaa aagatacttt cattgatcgg atcactgttc aactctctcg aaattacccg
     1801 aatcctgtat aaaaaatagc ggaatccata tatgtggatt ccgctatt
//
LOCUS       DAZUKD010000224         1764 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000224 DAZUKD010000000
VERSION     DAZUKD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.234"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1764
                     /locus_tag="ACG8N9_002899"
     CDS             4..1764
                     /locus_tag="ACG8N9_002899"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFS4898421.1"
                     /translation="MKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGSSR
                     DKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTD
                     GTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRS
                     FGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNS
                     FGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLG
                     FNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGII
                     TDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAIT
                     LVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVE
                     KYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWP
                     ASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGL
                     SEFKGVSPIDAYCGLKDTFI"
BASE COUNT          633 a          226 c          353 g          552 t
ORIGIN      
        1 actataaaag tagatttacg aaaaaaacca tataatttag atgaagatgc tatttcttgg
       61 attgaagaga cgattgattc tatgactttg gaagaaaaaa taggtcaatt gtttgttaat
      121 atgggatcta gtcgtgataa aaattacttg agtaatatgg tcaatcagta ccatattgga
      181 gctgtacgat ataatccagg aactgctgat gaggtatatg atcaaaacta tatcttacaa
      241 acagaaagta aaattcctct attaattgct gctaatacgg aagcaggtgg taacggagct
      301 tgtacagatg gtactgaagt tggtgtagaa gtaaaaattg gtgcaacaaa tgatgcgaaa
      361 tatgcttatg aattagggag agtttctggg attgaagcat cggctattgg atgtaactgg
      421 agttttgctc ctattgtaga tattaataga aattggagaa atcctatcat ttctagtcga
      481 tcttttggac aaaaccctga taaagtttta gaacttagtt tagcttatat gaaaggtatt
      541 caggagagtg gaattttgcc tgcagctaaa catttccctg gtgacggtat tgacgaacga
      601 gatcagcatc tatcttttag tattaatagt ttaggtacgg aagaatggga taattctttt
      661 ggaaaagtat actcaggctt aatagaagct ggattacctt caattatggc aggtcatatc
      721 catatgccag cttattcaaa acattttaat cctgatctga aagatgaaga tttacttcct
      781 gcaactttat caaaagaatt aatcactgac ttacttagag gaaaactcgg atttaatgga
      841 ttagtagtta ctgatgctag tcatatgtta ggaatgactg gggcaatgaa acgatcagaa
      901 ttattgccca cttctattgc agctggatgt gatctatttc tattttttaa tgatccagat
      961 gaagacttta attatatgat ggaaggctat cgtaaaggaa ttatcactga tgatcggtta
     1021 ttagatgcct taaaaagaat tttagggtta aaagctaaat tgggattaca caaaaaagca
     1081 aaagaacaaa ttttattacc taagcaagaa gctctggaaa aaattggttt aattgaaaat
     1141 aaatcaattt ttaaagaggt ggctgaccaa gcaattacat tagttaaaga taaacaagat
     1201 atttggccta ttgtaccaga aaaatataaa aggattttac tagtagatgt acatggggta
     1261 aaaggtgggt ttggtgaagt agttgctgga aatagacctc aagccattga tttattgaaa
     1321 cagattttgt cagcaaaagg atttgaagtt gaaaaataca tttcacctga agaagaattg
     1381 aaaggattat cgcaagaaga aaaaattaaa aatatagctg gaatatatgc tgctaagaga
     1441 ccaattaaaa atttaactaa aaaatatgac ttgatcatta atatagctaa tgttaatcca
     1501 ggcacagttc aacgaattgt ctggccagca agtaaaggaa ctccggatat accgttttac
     1561 attcatgaaa ttccaacaat ttttgtatct gtacagtgtc cgtttcattt ggctgatgta
     1621 cctcaagtaa agacttatat taatacttat gatggaaaac aagaaacttt agaaatattg
     1681 gtggacaaac taatgggtct ttctgaattt aagggagtaa gtccaataga tgcttattgt
     1741 ggtttaaagg atacatttat ttaa
//
LOCUS       DAZUKD010000225         1762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000225 DAZUKD010000000
VERSION     DAZUKD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.235"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1382
                     /locus_tag="ACG8N9_002900"
     CDS             <1..1382
                     /locus_tag="ACG8N9_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4898422.1"
                     /translation="GGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIE
                     VTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKV
                     TTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEV
                     DVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSG
                     KNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLG
                     YKNRKKENE"
     gene            1516..>1762
                     /locus_tag="ACG8N9_002901"
     CDS             1516..>1762
                     /locus_tag="ACG8N9_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4898423.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDK"
BASE COUNT          698 a          258 c          372 g          434 t
ORIGIN      
        1 ttgggggcac agtagatcta acagataatg ttactaactt gccagcgtta ccagaaggaa
       61 caacagtaac agatgttact cccggcggta caattgatac caacacacca ggtaattacg
      121 aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa gttccagtag
      181 aagtgacaga caatcgttct gacgccgata aatatgaacc aacagtagaa ggcgaaaaag
      241 tagaaattgg aggcacagta gatttaacag ataatgttac taacttgcca gcgttaccag
      301 aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggca
      361 attatgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc
      421 cagtagaagt gacagacaat cgttctaatg cagataaata caatcctgaa ggacaaaaag
      481 tgactacaga cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt
      541 taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata
      601 aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg
      661 ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga
      721 ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac
      781 caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa
      841 aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt
      901 ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc
      961 aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa
     1021 gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag
     1081 aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta
     1141 ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta
     1201 agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct
     1261 caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt
     1321 taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat
     1381 agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt
     1441 gcttttttat gatgggaaat aagtgttagt taaagatgaa tatacttttt atagaaagga
     1501 aaagaatgta attaagtgga aaaaatagta attattggtt caaaccattc agggctagcc
     1561 gcagctcgat ttctaaactt aagcgattca aattatgaag ttattctaat cgataaaaaa
     1621 tcagaaatta gttacttgga atgtgggaca tcgttattta taaaaggaga agtaagtaat
     1681 ttagaagatc ttttttatca aaatagaatg acactaaatt ctgagataaa aaagttattt
     1741 ctagagacag aagttgacaa aa
//
LOCUS       DAZUKD010000226         1760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000226 DAZUKD010000000
VERSION     DAZUKD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.236"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..1017
                     /locus_tag="ACG8N9_002902"
     CDS             43..1017
                     /locus_tag="ACG8N9_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4898424.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            1040..>1760
                     /locus_tag="ACG8N9_002903"
     CDS             1040..>1760
                     /locus_tag="ACG8N9_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4898425.1"
                     /translation="MKKSRLEYSLINSSISMFIYILRIVIQFVGRSFFIYFLGAKYLG
                     LNGLFTNILSFLSLAELGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIG
                     IVGVLIIPLLPLFIKNSTDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAI
                     NDFAFLFFMNIVQVIALYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVV
                     SLDKQTKDEVKKNVIGNMSSKI"
BASE COUNT          670 a          175 c          292 g          623 t
ORIGIN      
        1 tgtttaaatt agtaaataaa agaaaaggaa ttgaaaacta aaatgtgcag aataagtata
       61 atagttccag tttataatgt tgaaaaatat ttaaaaaagt gtgtcgattc aattctaggc
      121 caaacattta ccgattttga attattatta attgatgatg gttctacaga taattcagga
      181 agtatatgcg atgaacttgc gaaaacagac aaccgaataa aagtgataca taaggaaaat
      241 gggggtctaa gtgatgccag aaatattggt attgaggtag caaagggaga ttttattggg
      301 tttatagata gtgatgatta cattgatgaa gatatgtatg catttttata taacaatata
      361 ttgaaatatg atgcagagtt atcaatgtgt ggtatttatg acgtccataa gaataaagaa
      421 attaaaaagt taactccttt ttctcaattg gttactaaat ctgaagcaat tgaattagta
      481 ttagatggta agctagttgt tgctaatgca gtaagcaagc tatataaaaa agaattattt
      541 gagaatgtga gatatccaaa aggaaaaatt gctgaagacg cagcagtaat actgaaaatt
      601 atcaatcagt gtaataaaat ccatgttgat acttctcaaa aatattatta ttatcatcgt
      661 ggagatagta ttactagtaa aaaatttaca aaaaaagatt ttgatataat tgatgtatgg
      721 aatgagaatg aaaaatggat tattaaaaat tatcctcaaa tgtacgataa agtacatact
      781 agagtttgtt gggcttactt tgttgtgtta gataaattag ttttaactga agaaaaagga
      841 ctgtatcaag aaaaaaaaca agtaagagag tatttaattg ataatttctt ttttattata
      901 aaaaataaac gattaacttt tcaaagaaaa atatctatgt tattgcttac ttttggaatt
      961 gctgcgtata aaataccggt taaatatcag tataagaaag taaaggctgt taattaatgc
     1021 tttagttagg atgaattaga tgaaaaaaag caggttagag tattctttaa taaattcttc
     1081 tatatcaatg tttatatata ttttaaggat agtaatacaa tttgtaggta gaagtttttt
     1141 tatttatttc ttaggagcga aatatttagg attaaatggt ttatttacaa atatacttag
     1201 tttcttgtcg ttggctgaac taggcatagg ctcttctatt atttattccc tatacaaacc
     1261 attagcccaa aaggatgaaa gtaaagtaaa ggctttaatg aatttgtata agaagacata
     1321 tgaatttatt ggtattttta tcggcatagt aggagtattg attattccgc tgttacctct
     1381 atttattaaa aatagcacag acataccaca tatgtatcga tattatgtat tatttttatt
     1441 aaattcagtt cttagttatt ttttcacgta taaaagatca cttgcaatag cagatcaaaa
     1501 aaattatctg gttgcgatta atgattttgc ctttttattc tttatgaaca ttgtacaagt
     1561 aattgctttg tacttttatt ctaattttac tctatttcta atagtgcaga ttttgtttac
     1621 tctattttct aatataaata tttccagaac agttgacaag agatattcat atttaaaaga
     1681 gaaagatgta gtttctctag ataagcaaac aaaagatgaa gtaaagaaaa atgtcatagg
     1741 taatatgtcc tcaaaaatag
//
LOCUS       DAZUKD010000227         1685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000227 DAZUKD010000000
VERSION     DAZUKD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.237"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1082
                     /locus_tag="ACG8N9_002904"
     CDS             207..1082
                     /locus_tag="ACG8N9_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4898426.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1227..1379)
                     /locus_tag="ACG8N9_002905"
                     /pseudo
     CDS             complement(1227..1379)
                     /locus_tag="ACG8N9_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT          597 a          300 c          263 g          525 t
ORIGIN      
        1 gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa cacgctattc ttttactttt
       61 tctcacattc tctaagaaaa gaaaaggaga atgaattttt gacaattcga gcaaaagtga
      121 ttatcaaata tgtaaaatca gctacttttt tcttaaacgt ttctttatac taaatataaa
      181 taagtgtttc atgaaaggag aactagatga ataattggct tgagtatttt cctgaaaacg
      241 ttttggaacg cggttacagc tatcatctgc acggttttgt ccgtcatttg aactatacaa
      301 gcaagtactt gtccgcaaca gtttctggca cggaagatta taaggtagtg atcacttggg
      361 atgaaaagac caacatgact tgtgattgtc tatatgccat agaaggaaaa aaatgcaaac
      421 acatggctgc tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc aactactcac
      481 tttccgaact ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg gagcttttaa
      541 cagagattct tatagaacat ccgcaattta tcgaacgctt caaagttaag atgccatttc
      601 atgcaataaa ttattcagat aaattaacaa ctatcatcca taaatacgat catattatca
      661 agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag ttcatccaag
      721 aagcagttga atcgctcatt cagcaaaatg cctatcttcc cgcattcgaa ttgatcaatg
      781 aagtgattgc tactctcgaa acattctatt gggagcctga ggatgaacga acccttcttc
      841 tgatagagga ctgttattat ttatggaaag aattattagc agaagcacct catgcagaaa
      901 aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc tcttattcaa
      961 aacgctactc cataaaaatc ctgaaagaag actttcggga aaaagaattt tctaaccaaa
     1021 agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat
     1081 agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa
     1141 aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca
     1201 tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctggg
     1261 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
     1321 tccactgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
     1381 tctaatcttt aaagataaat attaaaataa cgattaataa tattaacagt ctttattttt
     1441 caccaccaaa aacctatctt tgcatatact aaatagacgt atatacacag ataggtttgt
     1501 cttgtgaaaa ataatgatta tccttaccaa aaaatgtttg tttttctaca tgttcgtact
     1561 tatcaaaaaa tatgatcctt attgaaatta ctggctattt atattccaac gacttcttgt
     1621 gttaattttt gttatctcat agtaaattat gtttcatttt cacttaattt agttgtcacg
     1681 tgttc
//
LOCUS       DAZUKD010000228         1658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000228 DAZUKD010000000
VERSION     DAZUKD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.238"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..1633
                     /locus_tag="ACG8N9_002906"
     CDS             383..1633
                     /locus_tag="ACG8N9_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4898427.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          543 a          319 c          297 g          499 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaatgacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatcctt
//
LOCUS       DAZUKD010000229         1651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000229 DAZUKD010000000
VERSION     DAZUKD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.239"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..250
                     /locus_tag="ACG8N9_002907"
     CDS             <1..250
                     /locus_tag="ACG8N9_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898428.1"
                     /translation="TIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRN
                     EILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(427..555)
                     /locus_tag="ACG8N9_002908"
     CDS             complement(427..555)
                     /locus_tag="ACG8N9_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898429.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(612..851)
                     /locus_tag="ACG8N9_002909"
     CDS             complement(612..851)
                     /locus_tag="ACG8N9_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898430.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1137..1451
                     /locus_tag="ACG8N9_002910"
     CDS             1137..1451
                     /locus_tag="ACG8N9_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898431.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          566 a          228 c          287 g          570 t
ORIGIN      
        1 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtgat
       61 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
      121 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
      181 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      241 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      301 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      361 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      421 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      481 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      541 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      601 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      661 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      721 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      781 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      841 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      901 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
      961 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
     1021 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
     1081 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg
     1141 atttttacag taattttatc ttaataatag caatattatt acttctaaat atttggtttt
     1201 ttgataagag tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa
     1261 aaaagtgggt atattcacag actatttttt acggaggggt tatttcaatc agtttacttt
     1321 cttcaacact ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta
     1381 ttggaatcct tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa
     1441 aaaataatta aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata
     1501 gcgaatgtga agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt
     1561 tatgttagga tgaattaatt tctctagagt ttataattcg ccgtttgtat aattaagcga
     1621 gacaaataaa aaagccattt atgttacact c
//
LOCUS       DAZUKD010000230         1641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000230 DAZUKD010000000
VERSION     DAZUKD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.240"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(491..1243)
                     /locus_tag="ACG8N9_002911"
     CDS             complement(491..1243)
                     /locus_tag="ACG8N9_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4898432.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1258..>1641)
                     /locus_tag="ACG8N9_002912"
     CDS             complement(1258..>1641)
                     /locus_tag="ACG8N9_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4898433.1"
                     /translation="QANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIE
                     SQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEIN
                     KKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          533 a          287 c          218 g          603 t
ORIGIN      
        1 gcagcgctgt agttggtgct gcaggaggac ctatttcggc cgttacagga ggtattggta
       61 atgtacttgt cggatgtgca ttaaagcctg ttcctgcaag ttaaatttat aaaaacagga
      121 aagtaattat ctcaaatcct atcaagtaca atcataggat tgcacttgac aggacaaatt
      181 ttttaactaa aaaatgaaaa acactttcta aaaattagta gaatttgcca aatctttcgc
      241 atcttccacc atacatttaa ttttaattcc cctttatggt acttaataaa aacaagaaaa
      301 gaactattca tttggccctt ttttggttcc agaccataga aatattcctc cagtaactaa
      361 tattgctgtt atgcacgtaa ctaataacag ctataaaaat tggtacaact ttaataggat
      421 tcagaaaaat ttgcactagt gaaggaatct attttattag aaaaatgcaa tctccctaaa
      481 gaaaaaacca ctaaacagtg ctttctttca acttctcctt aattttttgt gctttcccta
      541 aagtaaagtt tctggccaac tctgaatcaa aatatagtga acgtttttta aaatctattt
      601 ctttaacgtt aaagatattt actaaacacg ctcgactaat tcttgttaac ataggatatt
      661 tcttttcaag ctcactaatt cttccataaa attcgtactg cccatcttta gtacacaatg
      721 ataatctatg tggtaatttt gaagcttcta caaaataaat gtcatttata tcaaaagtaa
      781 atgtttgaga acctatggaa aaaacaaatg cttgattcgt aacctgtttt gttgcatcta
      841 tcctttcttg cgctaataac aataactcaa caatctcgtt tcgatactca tctagtgttt
      901 gatcttttgt tacaaatcct aacgtttcaa ctcttctttt tattgttacg ggtaacatct
      961 catcatgagt agttgtaaaa atgatttttg cttgaacatc atattttctg attacttctg
     1021 ctaattctat accgttaact tcatgattta aatcaatatc taagaaatat attccttgct
     1081 ttggtcggaa ttgtttgagg tattttttaa cttctaacgg actttgtgtt ttcaatacta
     1141 tcttaaatac tttatcgtga aaaagaatga aattatcaat aattcgttct aactgattta
     1201 attgtataat ttgatcttca cataatatta ttggataact cataaatcct ccttccctca
     1261 aaacaacaaa ataacttgag tagtaaattg atacggatcg ttttgatagt tcacaaacat
     1321 attgggattc tttttattga tttcctctat actagataaa cctaaccctt gatgtccctt
     1381 ttttgttgtt attccttttt tcattagagt atctatagag agtagtatct ctctacaact
     1441 attcgatatc gaaatttcta tctgtttatc atctcggtaa atagctagag acagttctct
     1501 atcttgactc tctatagatg cttcaattgc attatcgaga ataattccaa gcaccctaat
     1561 acagtcaaat attggaacag gaacttcaat aatagtactt ggacattcga atttacaaca
     1621 aatcttattg ttatttgctt g
//
LOCUS       DAZUKD010000231         1601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000231 DAZUKD010000000
VERSION     DAZUKD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.241"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8N9_002913"
     CDS             131..505
                     /locus_tag="ACG8N9_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4898434.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8N9_002914"
     CDS             complement(619..1248)
                     /locus_tag="ACG8N9_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4898435.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8N9_002915"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8N9_002915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          555 a          236 c          276 g          534 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat a
//
LOCUS       DAZUKD010000232         1589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000232 DAZUKD010000000
VERSION     DAZUKD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.242"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..1485
                     /locus_tag="ACG8N9_002916"
     CDS             286..1485
                     /locus_tag="ACG8N9_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4898436.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT          445 a          267 c          321 g          556 t
ORIGIN      
        1 cttcctttgc agcaaaaaaa gctcgtaatc taagagatga tggatagttt ttagaatata
       61 tatattttct tcaattccag aaaaaaatga ccaagctagt atgctaaaaa agtacgatgc
      121 ttctatgatc ttttttccgt ctttattaac agatgtttta attgatcaac gcatattgta
      181 atctacccta atttccatag ttcttcacca cttttaaaaa tagtaaaacc cctacaagta
      241 tgctatagta aattgctaaa gaaaaaccaa taaggtggga gatgtgtgcg cgatcaaaca
      301 agcggaaaaa tgattcgttt ttattataca agtttgcatt ttctttattg gatgatgttt
      361 tgttctgtct atgggtttgc gacattgttt ttgatttatt tcaagattga tccagctaaa
      421 atcggaggga tcttagcatt ggtcaatatt gtttcaacgg tgcttcagcc ttttgtcagt
      481 caagtgatca tcaagaaaag aaagatccca ctgatcaccg tgctgaagac gatgatagca
      541 ggattaggag tgattctctt agctggggta ttgattccga atttgccgat cattacttat
      601 atcttgggtg ggattttagt agtttctatg caatcatttc tcaatgcgct ggcatttgag
      661 tattcaaata ccggctacag gatcaacttt ggcttttcaa gagctggtgg ttcattttct
      721 tatgctgtaa cttctttttt actgggacag ctgttagcga tttattctgc cgcgattttg
      781 ccaatcttgg ttatagttga agcagtgata tttttcgtta ttttgcatct gctgccggat
      841 gtaaaaaatc aagaagtttt tcctatgaca aaagaacagg atcgctcagt ttctttgaga
      901 aaaaaatatc ctttcctgat tttgttattt ttaggtttca gttttctttt tactttccac
      961 accatgatca acagcttttt ggcacagatc tttgaaaata tccacggcgg cagtaaagaa
     1021 gttggcatcg cattgatgat cgccgcttta tgcgaattac cgggaatgat cttttttgaa
     1081 cagcttgtca aaaagaaatc cagcaaattt tggctgtctg ttgccagtat ttcatttgct
     1141 gtcagaggat tcatgatgct gctggccagt tcgatcctga tgatggaaat gacgcatctc
     1201 ctacaagcct tcagtttcgc tttgtttatt ccagcttcgg cttatttgtt caatcaattt
     1261 ctggcggatg atgatcgtgt atttggacaa acgatgatca tcatcgccac aactcttggt
     1321 ggggtgatag gaaatgtctt gggcggcagt ctgctgcagc atttcggtgt atgggagatg
     1381 ctgattttcg gtactggttt tgcggtgcta ggtgctttat taactatttt gggcattcga
     1441 aaaataccga agacaggaaa cgatagtgat tctcttgttg agtagcataa aaatacttag
     1501 atactagttt tatataaata acatatagtg attattggcg taattacaaa aaacgagaat
     1561 aacttaagta agcatttcta aataattga
//
LOCUS       DAZUKD010000233         1561 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000233 DAZUKD010000000
VERSION     DAZUKD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.243"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..1514
                     /locus_tag="ACG8N9_002917"
     CDS             48..1514
                     /locus_tag="ACG8N9_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4898437.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          433 a          290 c          329 g          509 t
ORIGIN      
        1 aagatgtata atattttctg aaaatttaaa gaattggggt atagattatg gaaaaacagc
       61 aagacaaggg cttcttcggg cagccgaagg gattgtcaac tttgttcttc actgagatgt
      121 gggaacgatt tagttattac ggtatgcgtg cattacttat ctattatatg tacgatacag
      181 ttgccaacgg gggacttgga ttaccacgag caacagcatt agctatcatg tcgatctacg
      241 gatctttgat ctacatgtct agtatcgtcg gcggatgggt atctgaccga gtgttaggag
      301 caaaacgcac tgtattcttc ggtggagttt tcattatgct tggccacatc gtcttggctt
      361 taccatttgg tgtaagtaca ttgtttgttt cgatggcatt gattattatc ggaaccgcct
      421 ttttgaaacc aaatgtttct ggcatggtcg gacatcttta ttcaaagact gacttgcgaa
      481 gagatgcagg attttcgatt ttttacatgg gtgtcaactt aggggcatta ttagcaccac
      541 taatcgttgg tacaatcggt caagaatata actatcattt agggttctct ttagctgcta
      601 ttgggatgtt ctttggatta ttacaataca tcatccaagg acgcaagaca ttagacggag
      661 tgggtacaac accgccgaat cctttgacaa ctgaagaacg taaaggattc atccgtaact
      721 tgatcatcgc acttgttgtt atcgctgcag tattcggcgg ggcacaacta actggtcatc
      781 tgacaattga cttttttgtc aatacaatca gtgtattggg gattatctta ccgttgtatt
      841 actttatcaa aatgctgact tcaaaagatg taactgctga agaaaaacca agagtatggg
      901 cttatatccc attattcctt gcagcaatca ttttctggtc attacaagaa caaggttcat
      961 ctatcttagc tttatttgct agtgaacgta cacaatctac gctatttggt gtaacgatcc
     1021 aagcaagctg gtatcaatca ttgaatccaa ttttcatctt tattctgaca ccagtattcg
     1081 taacgatgtg gacgaaactt ggtgaacgtc aaccatctac agtagtgaaa ttctctcttg
     1141 gacttttatt tgcaggatta tcgtacttgc tattgatgtt gccgggtatg ctttatggta
     1201 caagtggaaa agtaagtcca ttgtggttgt tgggaagttt cttcatcgtg gttattgctg
     1261 aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa attagcacca cgagcatttg
     1321 aatcacaaac aatcgctatt tggttcttag ctgatgcttc ttcacaagca atcaatgcac
     1381 aaattgcacg cttctacaca ccgggaacag aatctgctta cttcggtatt atcggtgcag
     1441 tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga accaatcaaa aaactaatgg
     1501 gctctattca ttaatcaatg aacataaaaa caggaattgc ttatttgcag cagttcctgt
     1561 t
//
LOCUS       DAZUKD010000234         1560 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000234 DAZUKD010000000
VERSION     DAZUKD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.244"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(335..408)
                     /locus_tag="ACG8N9_002918"
     tRNA            complement(335..408)
                     /locus_tag="ACG8N9_002918"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(372..374),aa:Ile,seq:cat)
     gene            complement(691..1338)
                     /locus_tag="ACG8N9_002919"
     CDS             complement(691..1338)
                     /locus_tag="ACG8N9_002919"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4898438.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
BASE COUNT          497 a          285 c          214 g          564 t
ORIGIN      
        1 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aaataggaaa agctattttt
       61 gtttggccgt tcttattact tgatgcagga cagcttcttc acaacctcta atcgttgata
      121 taaggaaggt acaggatttg aacctgcgcg ccggtattag ccggttcgcc ggatttcgag
      181 aatagggttt aaagctgatt ttaagtgttt ttcgcttatt atcagctttt tattatctaa
      241 aaaaacaact tttagagaca tccatatact gcttggggga gtcaaaaggg gagtcatttt
      301 tatattttat tcgcaattta aatcacttag agtatgtacc ctgtaggact cgagcctacg
      361 accggacggt tatgagccgt ctgctctaac caactgagct aagagtacgg aaagccatca
      421 caactactgc aaacaaaaag aatggacata gactagataa taatgggttt cattctttgt
      481 tgtggtggct aatttattat tacataaata ttattgaaaa aactagactt ttttgcatat
      541 taataaaaaa agagacacca tatagatgtc tctcaactat gtttctatgt gggcgcatac
      601 gtcccgatgt gtacattaaa aataggcgct tacgtccttc tataactaca tggtaactaa
      661 cgaacagaaa aatgtcaaca aaaatatcgt ttactttgta aaatattttt ttaaactttc
      721 atctacttca ttgaggacat ttccaggtgc cttcatattt ccagaaggat caaacttgtt
      781 aattcttcta attctatttt tactaatcgt ttggatatta agtggcatta cataggattc
      841 ttgtgtatat ttcttgtagc aattaaacac cttctgatat tttaccatat cttcagataa
      901 tgattttatt tctttaatta cttgttccct tgtctcgcaa accaaattat gttttacagc
      961 tcgtttttct acttcttcca ctgaaagctt ttcaccttta gcttcaatat ctttaataaa
     1021 actattcaaa tcgctttcat caatgatgcc tctatttaat gaaagtaaaa taattatcct
     1081 aagtttctct tttaatttat cagcttggct tataaattgt ttaatactag caacttctaa
     1141 tatttttcct acagatacat aattactttt cttttttgaa gagattggta ctacacatac
     1201 aacaccattt ttcgaattat ctttattatt taaaacaata gcaaaatgat taccagataa
     1261 ctcataaccc acgttcaccc caaaatcaac atatacaata gttcctcttt tataaaccct
     1321 ataattttta ttaggcatgc atctttcttt attaagataa aaacttttac tttgtaacca
     1381 gttgtgtaag tatttatact taggatatcc agataaagct tctccaataa acctaacatt
     1441 tgcttggtta catgctgctt ctatttcctc ttttttaact ataattaagc acctcttctc
     1501 actaactatt aaattaccgc atttttacaa gataatcaat aagttatgga caaatttgca
//
LOCUS       DAZUKD010000235         1536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000235 DAZUKD010000000
VERSION     DAZUKD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.245"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8N9_002920"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8N9_002920"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZUKD010000236         1532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000236 DAZUKD010000000
VERSION     DAZUKD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.246"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..536)
                     /locus_tag="ACG8N9_002921"
     CDS             complement(237..536)
                     /locus_tag="ACG8N9_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4898439.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            887..1519
                     /locus_tag="ACG8N9_002922"
     CDS             887..1519
                     /locus_tag="ACG8N9_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4898440.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          531 a          238 c          210 g          553 t
ORIGIN      
        1 acttacaaaa atgcaacatt aataaataac gatagtttca taaaatacac ttttaattaa
       61 cattattagt ttatactagc tgtttttgtg ttaattaaag gataaaatac aatataaaga
      121 aacatctgtt tctttattta gaataaaaaa atacagactc tgttcaaata aatgaaccta
      181 gcctgcatta tgagattgac tatacttttt aattcattga gatgaataac tcgtttttat
      241 aaatgcccaa aagtaatcgg tgccgaagcc attcctcgac ctaattcttt ttcaaatttt
      301 cctattgttg atatctccat ataaaacggt gatactggtt tagacaattc tcccctgatt
      361 gctaacggac gtaaactacg ttccaaatta tttatgactt taggataata ctgacctttt
      421 tctaagcctg tctttgcgtc tatcaaaagt atacgttcct tttctttgat ctctggattt
      481 aaaacgagat catataattt tagtaccatg ttttctattt cttcactttt agacatcacg
      541 atttcctccc aaaaatatat ttcttcccac tacattctat aaacaaagta tacgctacat
      601 ctagattttt ttaaatcatt actggattgt taaaaccttt caatacactg ttatggcaac
      661 gatttttatt ttaaaaacat tctaaattaa atcagtaaaa atttttattc taagattttt
      721 attagaatta cgcctaattt gtgtctctca gggcaattct aagaacgttt caagagatag
      781 cgacactatc gttatcgaaa aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg
      841 tttagctttt gttaatatga ctttatgaaa atggaggtaa ttaaatatga tttttcctaa
      901 agttaatgaa gcaagcttga ataaaggatc cttttttcat atctatgaaa acgaagattt
      961 tgaactgtat aatatccttt cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc
     1021 aattatttac aaatctattt tcaagtatgc attttttgct tccaatgatt ttcaaagaat
     1081 ggagattacc gactgtatat tcgaaaactg tgatttttca ggctgtatat tgacaaaaag
     1141 catgttgcat cgcgtgaaat ttgtcaattg taaattaaca ggtactaaat tgatggaaag
     1201 ttatattggg aacacgttat tcgagaattg taaaatggac tatgtaaatt tatctggttc
     1261 aaacataaaa gaaagtacct ttgagcactc tgtcttatca agcgcagatt ttgtagactg
     1321 ttccctcact aaaataatgt tttctactaa tgatttagaa aatgctagct tttttgatac
     1381 tgatttaaaa aatatcgatt taagtagcaa ctcctttgaa atgattgaag tacgctcgga
     1441 acatctaaaa aattgtacgc ttaatcagtt gcaagcactt ggttttgcta gaaaattttt
     1501 acaaataaaa gtagtataat caaaatgaat at
//
LOCUS       DAZUKD010000237         1525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000237 DAZUKD010000000
VERSION     DAZUKD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.248"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..718
                     /locus_tag="ACG8N9_002923"
     CDS             29..718
                     /locus_tag="ACG8N9_002923"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4898441.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            732..>1525
                     /locus_tag="ACG8N9_002924"
     CDS             732..>1525
                     /locus_tag="ACG8N9_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4898442.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAG"
BASE COUNT          487 a          210 c          321 g          507 t
ORIGIN      
        1 ataataaaaa attggagcgt ttaggataat ggataaaaag aggtttttag atcttatttc
       61 aaaaggtttg attgtttcat gtcaagcatt acctggtgaa ccattttaca aagaggaaga
      121 aagtttaatg ccttattttg ctttggctgc tcaaagggct ggagcagttg gtatacgtgc
      181 aaattctgtt cgagatatta ttgaaattag aaagtgtgta tcattaccaa tgattggaat
      241 tattaaaaga gattatccac cagaatctcc attcattact gccactatga gagaagtaga
      301 tgaattagta gaaaccggtg ttgaggtaat cgctcttgat tgcactctta gaaatcgaca
      361 tgatggacta tctataaatt cttttattac taaaataaaa gaaaaatatc ctcatcagtt
      421 atttatggca gatatttcta catttgaaga aggcaaaaat gcatataaag caggaataga
      481 ttttgtagga actactttaa gtggatatac tgaggaaagt aaaaaacaag atgggccaga
      541 tatagatttg attaaaaaat tggtagaaga agggatttgt gtaattgctg agggaaggat
      601 tcatactcca gaacaagcaa aagagataaa aaaaatagga gttgcaggta ttgttgtggg
      661 tggtgctatt actcgaccac aggaaattgc atcaagattt gttaaagcat taagttaggg
      721 ggagaaataa aatgtttaaa aaattttcac aattaggacg cgctttcatg ttaccaattg
      781 ctatattacc agtagccgga ttactattgg gactgggagg agcgttaaca aatgagagtg
      841 ctataaatgc ttatccattt ttagatcaac cgtggttgca tacaatttta agtattatga
      901 gttatgctgg aaatgcagtt tttgcaaacc tagcactaat ttttgcaatt ggtattgctg
      961 taggacttgc aaatggagat aaaggaacag ctggtttagc tggtggagta gcttatctag
     1021 tatatactgc aactattagt gggtttcttg aacttttttc agcaaaagat gcaacactag
     1081 atacaggtgt agtgggatca attgttgttg gtggtactgt tgcattttta cacaatcgtt
     1141 atagaaaaat tgaattacca caattcttag gtttttttgg aggatcaaga tttattccga
     1201 ttgtttctag tttagctgca atcattattg gttctttctt ttatttaatt tggccaccaa
     1261 ttcaaaatgg tttagtagtt gctggtgaac aaattgccca aatgggtagt ttaggaactt
     1321 ttttatatgg ttttttattg cgtttaacag gtgctgttgg actacaccac accatttacc
     1381 ctttgttctg gtatacatct ttaggtggta ccgaaactgt agctggatca acaattgctg
     1441 gagcccaaaa tattttcttt gcccaactag ctgatccaaa ccacactggt ctgtttactt
     1501 atggtactag attttttgca ggaag
//
LOCUS       DAZUKD010000238         1523 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000238 DAZUKD010000000
VERSION     DAZUKD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.249"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..272
                     /locus_tag="ACG8N9_002925"
                     /pseudo
     CDS             162..272
                     /locus_tag="ACG8N9_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(369..845)
                     /locus_tag="ACG8N9_002926"
     CDS             complement(369..845)
                     /locus_tag="ACG8N9_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4898443.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(858..>1523)
                     /locus_tag="ACG8N9_002927"
     CDS             complement(858..>1523)
                     /locus_tag="ACG8N9_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4898444.1"
                     /translation="MYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWL
                     YVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWA
                     FIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIES
                     VEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILG
                     KDN"
BASE COUNT          529 a          278 c          196 g          520 t
ORIGIN      
        1 acatctttca gcattttact atatttggta tatagtgcta aggttcatat tcgattgaca
       61 agagaatata aattgatcta tttctatttc ctaagttgag taaacataac tttgcccttt
      121 ataaatatct tccctctctc tcttctctct atattttata tttgaataat catatcaagg
      181 tattccaacg aaatgccaat ggatttcgaa gcttttataa ctttaaatta cggatcatga
      241 tttggcatgg ggaatcatta ctaataaaat aaaaaaatga acagaaagaa aacagttctt
      301 tctgttcacc aacttcattt gacaaatatc ctatttagct tattttatag ctttttatat
      361 acactttttt aaaaagtgat ttttccacat gaattcccat gagcaattcg accattatct
      421 attattaaat cattgaactg atttttctcc ggaaaaacaa ctataacaga agtatcttta
      481 ccagatttta ttattttgtc aaaatccaca tcaaccaacg aggtttcagg agtaacctca
      541 tcattttctt gaacacaaat cgaaaaaggc tctccttgta attcaactgt atcaattcca
      601 atatgaatca aataatcaat gcctgagttg ctttttatat taatagcatg tttagtagga
      661 aatatatttg ttactcgacc agccaatgga gcataaattt ttgaaccatc aggtatgacg
      721 gcaaaaccat cacctagtaa tttttgtgaa aagacaggat catttacttc tgtgatggaa
      781 atgatttttc cggaacaagg tgcataaaaa gtttcttgtt ttttcttttt gaaaatttca
      841 aacatattaa aaacctccta attatctttt cctaatattt gattgatttc ttgacttaat
      901 aagtcagctt tccctccaaa aacagcttgg atgccaccct tcacttcgaa gacagcagtt
      961 gcaccagtgt cttttattgt ttgtttttga ataatctccc cattcgtaac agcaacacgc
     1021 aaacgagttg cacaagcttc aacagactca atattttctt ttccaccaag agcttcaata
     1081 atgataagag actcttcatg taatgatgta tttttgttta ttactttttc agaatgaact
     1141 tctgattctt cttccattcc aggaatagct accttaaatt ttttgataaa gaatgtaaaa
     1201 acaaagaaat atataaatgc ccatgcaata ccaaaaggaa ttaattttaa ccaattagtt
     1261 ttgtcattcc cttgaagtac accgaataat aaaaagtcga tcaaaccacc tgaaaaggaa
     1321 ttccctacac gaatatttaa gatatcagca aaataaaaag ataatccatc taagaatgca
     1381 tgaatcacat aaagccaagg agcaacaaat aaaaacatat attcaactgg ttcagtaatt
     1441 ccagttaaaa atgatgtaag ggccccgcta aaataaagcc caccattctt tttacgattt
     1501 ttcttaggaa ttgcacgata cat
//
LOCUS       DAZUKD010000239         1503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000239 DAZUKD010000000
VERSION     DAZUKD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.250"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..383)
                     /locus_tag="ACG8N9_002928"
     CDS             complement(153..383)
                     /locus_tag="ACG8N9_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4898445.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(613..1431)
                     /locus_tag="ACG8N9_002929"
     CDS             complement(613..1431)
                     /locus_tag="ACG8N9_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4898446.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          527 a          247 c          203 g          526 t
ORIGIN      
        1 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
       61 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      121 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      181 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      241 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      301 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      361 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      421 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      481 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      541 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      601 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      661 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      721 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      781 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      841 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      901 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
      961 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1021 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1081 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1141 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1201 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1261 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1321 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1381 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1441 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1501 cca
//
LOCUS       DAZUKD010000240         1459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000240 DAZUKD010000000
VERSION     DAZUKD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.251"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG8N9_002930"
     CDS             complement(15..1154)
                     /locus_tag="ACG8N9_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4898447.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          382 a          316 c          263 g          498 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctg
//
LOCUS       DAZUKD010000241         1450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000241 DAZUKD010000000
VERSION     DAZUKD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.252"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1430
                     /locus_tag="ACG8N9_002931"
     CDS             <1..1430
                     /locus_tag="ACG8N9_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898448.1"
                     /translation="ARYLEFEKKNYVVLPKLYETLGLSKFKTRNYIIELNKEFTEFKT
                     KPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTIEKFAENHFL
                     SISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNGFSMPFRESV
                     MNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFFSDEYFKSSE
                     GMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEAHFHDIHMIS
                     QKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTSFTSKKQLQF
                     FFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVGICPVSYFEAPIHVC
                     IDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLEKFNKEQIIWKNPP
                     TPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          522 a          205 c          259 g          464 t
ORIGIN      
        1 tagctagata tttagaattt gagaaaaaga attatgtagt gctgcctaag ttatatgaaa
       61 cattaggttt gtctaaattt aaaacaagaa attatatcat agaattaaat aaagaattta
      121 ctgaattcaa aacaaagcca gagatccaaa tttcagaaga agcagagatc catgtcgaac
      181 gtctgaattt gacggatatc aaaaatttaa gaagaatcta ctttcaacga tcaaaaattg
      241 ctcgactgtt aacggagatc gtggattcaa aagtaacgat cgaaaaattt gcagagaatc
      301 attttttaag catatcaaga gcctatgtaa aaagaagaga attagtctct tttcttgaac
      361 agcatcaaat aaagttaaag aaaaacatat tagttgggga agaaataaac atacggaacg
      421 tattatttgg ggtatatttc ggggtcttta atggtttttc tatgccattt agagaaagcg
      481 tcatgaatga agcaaagagt atcgtgcaat attttgaata tctgttcaat ttaaaactgt
      541 ctgcaacaaa acgaatcaaa ttcaatttgc taaccactat tagtttgttg agggcctatg
      601 ggaatcaacc aatcggaaaa cgtttctttt cagatgaata ctttaagagc tcagaaggaa
      661 tgaagatcat caaggattta tcacatattt cttttcttca tatccctaaa gagcagatca
      721 ttgatgagat ttcttatatc atgttgtttt tgatcacgga agaagaattt gaattagatg
      781 gcgcatttgg acttgcggaa gctcattttc atgatatcca catgattagt caaaaaatgt
      841 cagtggaatt gattgatcaa ttaccatttt cgattgaaat ctctgaagaa gaaagactat
      901 atatacgaaa agaacttcgt atgggtatga tgcatgtcaa tcgaaaaaat tggatattcg
      961 actttgagat gactagcttt acgtctaaaa agcagctaca gtttttcttc gagacgtatc
     1021 caatattcag tacagcaatc caaaaggtca tacaagagaa taaacgctat ttcttgggaa
     1081 aagatgaacg tttcatgatt cgcttatttt atgattatct ctttttattg gtcggtattt
     1141 gtccggtatc atattttgaa gcaccgattc atgtctgtat tgatttttcg caaggatctg
     1201 cttataccga ttacattatt tctcaagtga aaggatttaa aaattacaat attgtgattg
     1261 agagtcgaat cacttctcgt acgaatattt atttatctga ttgtttactt gaaaaattta
     1321 ataaagaaca gataatttgg aaaaatccac cgactccaga tgactgggaa tattttggaa
     1381 ataccatcat cagagaaaaa aatagacatt taactgaaaa aatcagctag tatttatgga
     1441 tggcagcttt
//
LOCUS       DAZUKD010000242         1425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000242 DAZUKD010000000
VERSION     DAZUKD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.253"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1425
                     /locus_tag="ACG8N9_002932"
                     /pseudo
     CDS             5..1425
                     /locus_tag="ACG8N9_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          590 a          223 c          319 g          293 t
ORIGIN      
        1 ggtcatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccagag
      121 aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agagcgaatc gaaaataaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 agtagaaaaa cttgaaagac aagaagagat aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg aagtacaagc
      481 aatcattgac agcggacgag tgacgcaatg gaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta
      661 ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaa
//
LOCUS       DAZUKD010000243         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000243 DAZUKD010000000
VERSION     DAZUKD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.254"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8N9_002933"
     CDS             complement(150..1337)
                     /locus_tag="ACG8N9_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4898449.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZUKD010000244         1408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000244 DAZUKD010000000
VERSION     DAZUKD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.255"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1225)
                     /locus_tag="ACG8N9_002934"
     rRNA            complement(<1..1225)
                     /locus_tag="ACG8N9_002934"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          316 a          394 c          272 g          426 t
ORIGIN      
        1 accaatacac ctaaaggtgt actccacagc ttcggtaata tgtttagccc cggtacattt
       61 tcggcgcagg gtcactcgac tagtgagcta ttacgcactc tttaaatggt ggctgcttct
      121 aagccaacat cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg
      181 ggaccttagc tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt
      241 ctgactcccg gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat
      301 gggcccctag tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa
      361 agctatttcg gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca
      421 cctcatcccc gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc
      481 ttcaacctgg acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc
      541 ctattcagac tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt
      601 aactcgccgg ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt
      661 gtaggcacac ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc
      721 tcacggtact ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc
      781 cggattccga cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca
      841 catttcgtct acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct
      901 gaaagaacta ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt
      961 ttcccgtttc gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact
     1021 tagatgtttc agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca
     1081 tcctattaaa gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag
     1141 ctccccaaag catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc
     1201 accgtgcgcc cttattcact taaccttatc aaccttacgg ttgggttttg atccttcttc
     1261 tagcgataga agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag
     1321 ctcacaaatc agcctgtacg gcaaaaagat cgatcgtctc ataaaatcaa agattttatg
     1381 gccgtccgcc tattttgctt caggctaa
//
LOCUS       DAZUKD010000245         1366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000245 DAZUKD010000000
VERSION     DAZUKD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.256"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..100)
                     /locus_tag="ACG8N9_002935"
     tRNA            complement(16..100)
                     /locus_tag="ACG8N9_002935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64..66),aa:Leu,seq:caa)
     gene            complement(118..189)
                     /locus_tag="ACG8N9_002936"
     tRNA            complement(118..189)
                     /locus_tag="ACG8N9_002936"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Gln,seq:ttg)
     gene            complement(199..271)
                     /locus_tag="ACG8N9_002937"
     tRNA            complement(199..271)
                     /locus_tag="ACG8N9_002937"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:His,seq:gtg)
     gene            complement(333..417)
                     /locus_tag="ACG8N9_002938"
     tRNA            complement(333..417)
                     /locus_tag="ACG8N9_002938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Leu,seq:caa)
     gene            complement(438..508)
                     /locus_tag="ACG8N9_002939"
     tRNA            complement(438..508)
                     /locus_tag="ACG8N9_002939"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(474..476),aa:Cys,seq:gca)
     gene            complement(554..625)
                     /locus_tag="ACG8N9_002940"
     tRNA            complement(554..625)
                     /locus_tag="ACG8N9_002940"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(591..593),aa:Gln,seq:ttg)
     gene            complement(637..709)
                     /locus_tag="ACG8N9_002941"
     tRNA            complement(637..709)
                     /locus_tag="ACG8N9_002941"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(674..676),aa:His,seq:gtg)
     gene            complement(712..785)
                     /locus_tag="ACG8N9_002942"
     tRNA            complement(712..785)
                     /locus_tag="ACG8N9_002942"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Trp,seq:cca)
     gene            complement(792..872)
                     /locus_tag="ACG8N9_002943"
     tRNA            complement(792..872)
                     /locus_tag="ACG8N9_002943"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(836..838),aa:Tyr,seq:gta)
     gene            complement(874..949)
                     /locus_tag="ACG8N9_002944"
     tRNA            complement(874..949)
                     /locus_tag="ACG8N9_002944"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(914..916),aa:Phe,seq:gaa)
     gene            complement(985..1057)
                     /locus_tag="ACG8N9_002945"
     tRNA            complement(985..1057)
                     /locus_tag="ACG8N9_002945"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1022..1024),aa:Asp,seq:gtc)
     gene            complement(1067..1139)
                     /locus_tag="ACG8N9_002946"
     tRNA            complement(1067..1139)
                     /locus_tag="ACG8N9_002946"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1104..1106),aa:Val,seq:tac)
     gene            complement(1155..1226)
                     /locus_tag="ACG8N9_002947"
     tRNA            complement(1155..1226)
                     /locus_tag="ACG8N9_002947"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1191..1193),aa:Glu,seq:ttc)
     gene            complement(1241..1329)
                     /locus_tag="ACG8N9_002948"
     tRNA            complement(1241..1329)
                     /locus_tag="ACG8N9_002948"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1293..1295),aa:Ser,seq:gga)
BASE COUNT          383 a          362 c          315 g          306 t
ORIGIN      
        1 ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg
       61 attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg
      121 gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc
      181 tataccccaa gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
      241 tccggtgcgt taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca
      301 ataaaaaact aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg
      361 ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag
      421 ttgatttatt agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac
      481 ctctgcctta ccacttggct ataccgccat tatactatta tcaatctaca aaataaagaa
      541 taaattatag aaactggggt agctggattc gaaccaacgc atgagggagt caaagtccct
      601 tgccttaccg cttggctata ccccaagaat aaaaagggcg aatgatggga atcgaaccca
      661 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atggcagggg
      721 cagcaggaat tgaacccaca ctaacggttt tggagaccgc tgttctacct ttaaactatg
      781 cccctataaa atggaggaga gtggattcga accactgaac cctaaggaac ggatttacag
      841 tccgtcgcgt ttagccactt cgctactcct ccatggtggc gcgggacaga atcgaactgc
      901 cgacacacgg agcttcaatc cgttgctcta ccaactgagc taccgcgcca tattttaaaa
      961 ttattaattg aataacaaga acgacggttc cgacgggaat cgaacccgcg atctcctgcg
     1021 tgacaggcag gcatgttaac ccctacacca cggaaccagt tcattatgga ggttaacggg
     1081 atcgaaccgc tgaccctctg cttgtaaggc agatgctctc ccagctgagc taaacctcca
     1141 acattcaatt aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg
     1201 tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta cggagagtaa gggattcgaa
     1261 cccttgagac agtttgcacc atctacatga tttccaatca tgctccttcg gcctctcgga
     1321 caactctcca gagtgaaaga agctcaatac tctttctcaa aatgag
//
LOCUS       DAZUKD010000246         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000246 DAZUKD010000000
VERSION     DAZUKD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.257"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8N9_002949"
     CDS             82..>1364
                     /locus_tag="ACG8N9_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4898450.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZUKD010000247         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000247 DAZUKD010000000
VERSION     DAZUKD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.258"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8N9_002950"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8N9_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS4898451.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZUKD010000248         1354 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000248 DAZUKD010000000
VERSION     DAZUKD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.259"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..>1354
                     /locus_tag="ACG8N9_002951"
     CDS             105..>1354
                     /locus_tag="ACG8N9_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4898452.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKT"
BASE COUNT          439 a          275 c          255 g          385 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agac
//
LOCUS       DAZUKD010000249         1283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000249 DAZUKD010000000
VERSION     DAZUKD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.260"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..401
                     /gene="spx"
                     /locus_tag="ACG8N9_002952"
     CDS             <1..401
                     /gene="spx"
                     /locus_tag="ACG8N9_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFS4898453.1"
                     /translation="YTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAA
                     TEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFN
                     EDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            415..771
                     /locus_tag="ACG8N9_002953"
     CDS             415..771
                     /locus_tag="ACG8N9_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4898454.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          461 a          191 c          239 g          392 t
ORIGIN      
        1 tatatacttc gcctagctgt acttcctgta gaaaagcaag agcatggttg caagcgaatc
       61 aacttgaatt tgaagagaag aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa
      121 agatcttggc agcgactgaa ggaggagttc aagatattat ttccactcgg tcgaaaattt
      181 acgaaaagct gggcatagat tttaacgaat taactttaaa gcaaatgatc gtcttattca
      241 aagaatatcc ttcattattg agacgaccta ttttgattga tgaaaaaaga atactaatcg
      301 gatttaatga agatgagatc cggtcatttg tccctcgtga aattcgatgt gttgccagaa
      361 atctgatcta tttacaaaac aataacatct taaattcata gggagggagc cttcgtggaa
      421 gtgaaagtct atggatcagc gcactgtgta acaagtctga aggcgaaaca atggttagaa
      481 caccatcaat tgccttatca atttgttgac ttagaagaac gcgatctaag tacagaagaa
      541 gcagaagaat tatgcagttt tgaagaagtg caagcagaac agctatttgc gacttggtct
      601 gaggcttttc acaacattgc gattgacctt tcctgtgtag gaaaaaaaca actgattttt
      661 ttatgcacac aacataccaa tctcctcaga cgtcctttga ttattatcga tgaagttcta
      721 tttattggat acaatgagca gttgatggaa cagcttatct taaataaata gactttaact
      781 gttttaatca agtcactgag catttcatac agttgtaaca gtcgaacact tagcatcaag
      841 cgtgttatgt gttctttttt tgactttatt tttctactat agagacatgc tcaatttatg
      901 tggtcaaaat tcaaatctag gtggggatat agagttaaaa atatattgac atgtgtaaat
      961 ttacatgata tattcatttc tgttgtttta agtagcaaag caataaaata gtattttaaa
     1021 aaacatataa aatagttgtt gacatgttag aaatgacatg ataagataac tgagttgtta
     1081 aaaaagaacc acttgtgaag cttgattcta tcgaaaaaaa cttcaaaaaa gttcttgaca
     1141 aataacaaat gataagttat gataaatgag ttgctgtaag atttcgaaaa acgaaatcaa
     1201 aaaaactttt aaaaaagagt tgacaattac ttgtctctct gatatgatat aaaagttgct
     1261 acggcagcca aaaacgaatc ggg
//
LOCUS       DAZUKD010000250         1283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000250 DAZUKD010000000
VERSION     DAZUKD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.261"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..479
                     /locus_tag="ACG8N9_002954"
     CDS             330..479
                     /locus_tag="ACG8N9_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898455.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            747..1133
                     /locus_tag="ACG8N9_002955"
     CDS             747..1133
                     /locus_tag="ACG8N9_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4898456.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          468 a          192 c          227 g          396 t
ORIGIN      
        1 tattttttaa cacgatctta gacgttgtga caaaaacgtt tgtttatatt cctatgttgt
       61 tcgtgcgttt ttttctttat attgctaagt tatttataaa gaggtgagca tatgatttat
      121 attcaagcaa tagtatttgt aaccttgatg attgggctac ttatcggatt aaaagaaaat
      181 agctaaacca tttaagaaaa aataaataag gaaaatgata aatgcttttt aattgcatca
      241 aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt taaaggaaac tttcaagaac
      301 taaattacat tacaaaggag atgaatttta tgacgtttac aaaaacaaat atgcaatacc
      361 gcgtagcttt tgatacaact tctaaccgtt ttatggcgat tgatgcacaa aacgaaaata
      421 acgttgcgtt tggtattaca atcgaacaag cagtccgagc attaaacgaa tctagataag
      481 gagtgaacca ttccaaatgg cgactgatga gatctcggaa aatcaataaa agacgtttgt
      541 aacggtaatc cgcgacaaac gtctttttgt attcattaca tatatttgga aattgtctct
      601 ctttatctct agacctaaac aaaaacacaa gttctaatat caaccatctc gttttttatg
      661 acaattttgt tagctgtttc gatgataata ttccgttatt atctgtataa tatttaatat
      721 aataaaaata aaaaaggaga tctttcatga aaaaaatttt agggttgatc gttatcatat
      781 tgattgcttt tataggatgg aaaggttggg actactatca gtctacttat gtaggtaaag
      841 attattatgc ggtagtcaaa gctccaatgc ctgcagaaac tgatatcaaa gcagataatg
      901 gggaagtaat tggaagaggt tttaaataca acgtagatgc ttatgctgaa aacggtgaaa
      961 aaagacagtt agatttcgat gtcattactt ctggtgactc tgccaatggt tctgcttaca
     1021 ccgaaggaac gatcttgcag ttgaaagcaa gcgaaaagcg gatcatagag aaaaaagtaa
     1081 ttaccagcga taaggtaccc tcatctgtaa aagaaaaatt aggcctatct taagctaaga
     1141 atatatcgca aaaaagcact cgaagtgagt gcttttttgc ttactgattc tagaaaacaa
     1201 aaaaacagaa ttaaccttcg aagtcagcct cactgttctt aaagtcctaa aacaaactaa
     1261 agagcaaggc aatcaatcga ttt
//
LOCUS       DAZUKD010000251         1281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000251 DAZUKD010000000
VERSION     DAZUKD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.262"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..773)
                     /locus_tag="ACG8N9_002956"
     CDS             complement(120..773)
                     /locus_tag="ACG8N9_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4898457.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1061..>1281
                     /locus_tag="ACG8N9_002957"
     CDS             1061..>1281
                     /locus_tag="ACG8N9_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4898458.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEK"
BASE COUNT          422 a          224 c          150 g          485 t
ORIGIN      
        1 tctcgtctcc aaatatgttt taggaaagat aaaaaaaatt aatgaatgaa ctaatataat
       61 tattattcca agaacagaaa atatttgtat gaacattttt ggaaatatct ttaatttttt
      121 cattttctct ccaatttata tcctacattt cttatagtag ttatacagtc taattgtaat
      181 ttttttctaa gttcttttat atatacatct attactcgat cataaggagt ttcttcgcta
      241 tctttccata cataatcaat aatttgcatt cttgttaaca cttgtccatt attatccaat
      301 aaacatttta atacttctag ttcttttgca tttacatcta tttcttcacc atttatttta
      361 gctgtatagc tattaaaatt aaccgataga tccttatatt caaacttctc taaatgtcca
      421 taattcttct taattaaaga atctattcta gcttttaaga ccggcaatga aaatggcttt
      481 tctacatatc cgtctactaa attagtaaat gcatcaattt tatattcttc atcactaaat
      541 gcagtcaaga ttagaattgg taaattgctt ttctttctaa tttctttcaa cacttctaaa
      601 ccatttataa aaggtatctg aatgtctaag ataaccaaat ttatatcgct attaaatttt
      661 gacaaggctt ctcttccatc tttagcttga ataacagtat atccaaattc tgaaagatat
      721 tcacttattc cctctcttat catattatca tcttcaacag ttaatatttt catatatacc
      781 tccacttaag cctatacact agatattata cagcatatta tggctctata atatctagtg
      841 ttggtgacac cttctgttgc caatactttt tcttttttct aataaatgca aacagacacc
      901 ttgaaatcct ttagaataaa gggttctata atattttgtg ttgttggaaa atcgaaggcg
      961 attttctact aacacttttt cttcgtttca actcaaaaag agactcactt ttggtaaaat
     1021 ttagtcgacc aaaacatcac cctaccaaag gagtcgctct atggataatc atactagaaa
     1081 attactcgga ttaacagata aacatttatt ttttgatgaa gaatggctga ttgaaaaaga
     1141 atataaaggc gttcaagcgc agtttattaa agggagattg gatgactcac cttcccattg
     1201 tgaacactgt ggatcgattc gtattatccg caacggttcc tatacgacac gtactcagat
     1261 attgaaagtc aaagaaaagt t
//
LOCUS       DAZUKD010000252         1270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000252 DAZUKD010000000
VERSION     DAZUKD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.263"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..416
                     /locus_tag="ACG8N9_002958"
     CDS             21..416
                     /locus_tag="ACG8N9_002958"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4898459.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            413..>1270
                     /locus_tag="ACG8N9_002959"
     CDS             413..>1270
                     /locus_tag="ACG8N9_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4898460.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKIGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKNKIINLIYPKLIEDEHGE
                     YFSPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDII"
BASE COUNT          505 a          140 c          242 g          383 t
ORIGIN      
        1 cattatttag ttaaaaagat atgttttgca aagtatgtga tagaaaagag ggattctgtg
       61 gcttcagaat agatatcaaa gaattggcaa aagtttctga tattttttta tttactacat
      121 tacaagaagg aatgccaaga tctatgatgg aagctatggc aagtggattg ccatgtatag
      181 caagtaaaat ccgaggtaat gtagaccttt tagtggaagg aaaaggtgga tatcttgttc
      241 cagctaaaga tgcagaatta gtagcagagt gtttgagaga actaatcaat aataaatctc
      301 ttagaaagta tatgggaaat gagaaccttt ccagaattaa agaattcgag gttagtatcg
      361 taaaaaaaag aatcaaagaa atatatgaag aagttatagg tgagagtgta aaatgaaaaa
      421 tccaatgttt tcaataatag tacctatcta ttgcgttgaa aaatatttga aagattgctt
      481 agattcaata agaaatcaga catttaaaaa ttttgaagca atactagttg atgacggtag
      541 tcctgacgaa tgcccagaaa tatgtgacta ttattcccaa atagatggac gtttaaaagt
      601 aattcataag aaaaatggtg gaataataga tgcacggaaa ataggagtca ctagtgctat
      661 gggagagtat atagtttttc tagatggtga tgattggctt gcaaatactt atttagaaaa
      721 atatgcggaa gtaattacta agtacaatcc agatataata tgtagtgggt attttgcatc
      781 acgttccaac aaaattagaa aaagagaatt gtcttgctcg tttggatatt atgacaaaaa
      841 taaaattata aatttgattt atcctaaatt aattgaggat gaacatggag aatattttag
      901 tccacaatta tgggcaaaag cttttagaac tactctgtac aaggagcaac aacaagtatc
      961 tgggcaagtt agtgttggtg aagatcatgc atgtacaaaa ccatgtattt accatgcaaa
     1021 ttcgatatat atgatggaaa aatgtttata ttattacaga ataaacgaaa attctataac
     1081 aaaaaaacca caaccatatt attgggaagg accaaaggct attggagagc attttgaaaa
     1141 gcagattgat atgaataagt atgattttca agatcaagta tatagaaatg ttacacacaa
     1201 tttatttaat gttgctgttt cacaatttaa taaacaggac aaatatttaa atataaaaaa
     1261 agatattata
//
LOCUS       DAZUKD010000253         1265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000253 DAZUKD010000000
VERSION     DAZUKD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.264"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1007
                     /gene="scrK"
                     /locus_tag="ACG8N9_002960"
     CDS             135..1007
                     /gene="scrK"
                     /locus_tag="ACG8N9_002960"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4898461.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          387 a          228 c          289 g          361 t
ORIGIN      
        1 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
       61 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
      121 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
      181 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
      241 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
      301 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
      361 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
      421 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
      481 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
      541 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
      601 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
      661 actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac
      721 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
      781 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
      841 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
      901 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
      961 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     1021 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     1081 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     1141 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     1201 tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat atatctttgc
     1261 ttctt
//
LOCUS       DAZUKD010000254         1253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000254 DAZUKD010000000
VERSION     DAZUKD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.265"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..459)
                     /locus_tag="ACG8N9_002961"
     CDS             complement(61..459)
                     /locus_tag="ACG8N9_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4898462.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(456..692)
                     /locus_tag="ACG8N9_002962"
     CDS             complement(456..692)
                     /locus_tag="ACG8N9_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4898463.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(852..1136)
                     /locus_tag="ACG8N9_002963"
     CDS             complement(852..1136)
                     /locus_tag="ACG8N9_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898464.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          392 a          225 c          190 g          446 t
ORIGIN      
        1 aaatcagcat attccttaat gacatgctga tctaattgag aaataaagtt tttaattcgt
       61 tcattttatt gcggtttgtc gtaaataact ggttatcttg gactttagtg aatcgaaatc
      121 caatgaacta gtggtaatca ttatgataaa atcaacagct tcttgacgag aaaaagcaac
      181 tggacaatta ttcaattgaa gaaacaacaa catggcaaca agagcagttc gtttattgcc
      241 attatgaaaa ggatgttttt tagctaaatt aattgctaaa atcgccgctt tttcatagat
      301 ttcaggatag agttcttgat tgaatactgc ttgtgaaggc tgtttgacgg ccatatctaa
      361 tgcatttgca tcttttacac cgatcatttc gcctggcgag tgacgtaaaa taactttcgc
      421 gttgattttg ataatttctt tagtcgaaag atacctcata aatcaaccaa atcttttaac
      481 gcgtcttcat attgattcat gatcgaatca actaatgaat caatatcagg ttcttcttgt
      541 gctttgatca ttcgtacaga accatcagat tgaaaaacat aactgattgc gtcacctgtc
      601 tgcacaccca tgaattcttt tacttctggt gggatagttg ttacatcgct attgccagat
      661 tttctaattt tatacttttt catttttttc atatttcgcc accctctata ccattctcat
      721 tctatagtaa gtacattata tccaagtata tacttttaat caatgtatat acttttattt
      781 tgctttttct tacgaaaaaa tgacaaagaa aaaagtttgt gacatgactt ttgtcacaaa
      841 ctctcgtatt cttaaccaaa atattgatga aatacttgtg taataactgg tagaaaataa
      901 ataatgatca tagccgctac caccaccaac aattgttttc gctgtttctt tcgctttagc
      961 atgcttgtcg ttttatgaaa aaaataagta gcgcttttag ctcctgcagt caaagtcgat
     1021 aaaatcaacg ttggtgaata tgctgtttga ttgtgcgtca atgacaacaa cttcgtcccg
     1081 taatcaggat actttagaaa tgcttggtac acagccacaa tcgtgattgc tcccataaga
     1141 taatttaaaa atgaatgcac ttttttcaaa taaaaaaact ccttgctatc tatataaaat
     1201 cagcctaccc caacgttttt gcttgattca ctgatatttt ttagttttat tgg
//
LOCUS       DAZUKD010000255         1246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000255 DAZUKD010000000
VERSION     DAZUKD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.266"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8N9_002964"
     CDS             200..742
                     /locus_tag="ACG8N9_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898465.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG8N9_002965"
     CDS             898..>1246
                     /locus_tag="ACG8N9_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4898466.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZUKD010000256         1146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000256 DAZUKD010000000
VERSION     DAZUKD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.267"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..330
                     /locus_tag="ACG8N9_002966"
     CDS             64..330
                     /locus_tag="ACG8N9_002966"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4898467.1"
                     /translation="MTSTYASFDKDNLYYDFPSVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
     gene            412..708
                     /locus_tag="ACG8N9_002967"
     CDS             412..708
                     /locus_tag="ACG8N9_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898468.1"
                     /translation="MANNLHYVTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFS
                     LNFIMIDYYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
     gene            718..924
                     /locus_tag="ACG8N9_002968"
     CDS             718..924
                     /locus_tag="ACG8N9_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898469.1"
                     /translation="MDGEEYPYKVAFDDLIQLSNLPKDSNMKIAGEFFAPGFLVGLVI
                     SSIATGCFLFLLFLDYIKKTEDIP"
BASE COUNT          394 a          184 c          204 g          364 t
ORIGIN      
        1 acaatcaacc aaactctaac agaatttgaa gtatatcaag aatattggga accactaaaa
       61 aaagtgactt caacttatgc atcctttgat aaagacaatc tctactatga tttcccctca
      121 gtatcaggat ttagttcaat gaacaataaa aaatatttac taatgttaac gagattaggg
      181 tactcaacaa gtaacgtccg tgctaaacgg aatggtggta cagttattac ggatattttg
      241 ctaagcaacc aataccaagt aattcctaat caagaaatcc agcatcaagc gactagcggt
      301 gtaaagatta tagaaagcga agtgagttaa acctcagata ttgcttcttt ttcatttgac
      361 gttctagaag taacaaaaga tgagttactt tatgtttatt ttcccgtcaa cttggccaat
      421 aatttgcact atgtgaccaa tttggaaagt cgacaatttg atccctgctg gcatcaaatt
      481 gatgctcaaa cgaaagaatt gttgctaggt tgggaagaaa atcaagtagt tcgtgatttc
      541 tcattaaatt ttattatgat tgattactat aatttggcga catcaatgca acggttcccg
      601 aattatggta tggacataga ttcattcaaa cagaaaagct tttctggaga aattcaaaat
      661 gaggaaagca ataatcttgt tatacctatt ctctatgaac caggttaggt ttttactgtg
      721 gacggggaag agtatcctta caaagtagct tttgacgatt tgattcaatt gtctaactta
      781 ccaaaagata gtaatatgaa aattgctgga gaatttttcg caccaggatt cttggtaggc
      841 ttggtaatct ctagtattgc aactgggtgc tttttatttt tactttttct ggattatatc
      901 aagaaaacag aggatattcc ttaggaaaat cacaatctaa atcttcccta gaaactatag
      961 aatgacaagg gggatataag caaattccta aatgattaaa tagggtgcgt tactagttag
     1021 ctccatagta agattttttc aaaattttct tgaggttgga tcaaaaaggg ctcttttcaa
     1081 ttagattaaa gacactcata caaaatagag aaacgaactt ataatcgcgt tcgtttccct
     1141 atttaa
//
LOCUS       DAZUKD010000257         1141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000257 DAZUKD010000000
VERSION     DAZUKD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.268"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..800)
                     /locus_tag="ACG8N9_002969"
     CDS             complement(<1..800)
                     /locus_tag="ACG8N9_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4898470.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFT"
BASE COUNT          329 a          234 c          188 g          390 t
ORIGIN      
        1 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
       61 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
      121 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
      181 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
      241 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
      301 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
      361 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
      421 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
      481 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
      541 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
      601 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
      661 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
      721 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
      781 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
      841 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
      901 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
      961 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
     1021 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
     1081 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
     1141 c
//
LOCUS       DAZUKD010000258         1133 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000258 DAZUKD010000000
VERSION     DAZUKD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.269"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1103)
                     /gene="dnaN"
                     /locus_tag="ACG8N9_002970"
     CDS             complement(<1..1103)
                     /gene="dnaN"
                     /locus_tag="ACG8N9_002970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4898471.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFI"
BASE COUNT          348 a          211 c          209 g          365 t
ORIGIN      
        1 tgaataaatt gaacgccatc ttccgttggt tccaatgtaa atggacgaat agcagagata
       61 aatttcactt tgatgctcat atctccaaat gcacgcaacg ctgctttcat ataatccgga
      121 ttgaaagaaa tatctaatgg atcgccactg cttgctgtat aactcaagct ttcttctact
      181 ttcccgattt ctggcgaatt tccgtataaa acaaccgcat ctggacgaat agataaacga
      241 acgatgttgt tacggccttc atgagaaagt aaagaagccc gttcgatcgc cgctaaaaag
      301 ctaggaacag aaaattcaac ctctgtatta aaacttgaag gaatcaaacg attggtatct
      361 ggatagtttc cttctagcaa acgggaatag aaatacatcg tttctgtttt gaacaacact
      421 tggttttcca taatgctgat ttcgacgatt tcttcttcat ttgttaatga gcgagataat
      481 tcgatcaaac tttttccagg aataacaata tcaaaatgat cagccgcttg ttctactgga
      541 atcacgcgtt gacttagacg gtgagaatca gtagctacag ctaataaaga attatcagat
      601 aaaatgaaat gcacacctgt caagattgga cgactctcat gttgtgagac agcaaaaact
      661 gtttcgttga ttagtttagt caatacgtgt acaggtaatt tcatctggtt atggctttcg
      721 acaacaggaa gatgaggata gttatctgca tctaatccat ttacgataaa attcgctttt
      781 ccagaagtga tcgctacttg tttattttct aaaacttcta aagtaaatgt ttcttcagga
      841 agtctccgaa tgatttcgct aaagaaacgt gcttgtaaaa caatggaacc agtagattcg
      901 atttgcatat ttgctttttc gttttcaaca gacaaaaaag tttcaattga tatatcagcg
      961 ttgctccccg tcaaagtcaa accttcttgt gtcagtgtga tttttacacc tgtcaaaata
     1021 gggatcgtgg ttttgcttga aatagctcgt tgaacagttt gcaattcctg cataaagcta
     1081 gctcggttta aagtaacttt catgatgtat ctcctttgaa tgtttgttta ttt
//
LOCUS       DAZUKD010000259         1112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000259 DAZUKD010000000
VERSION     DAZUKD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.270"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..542)
                     /locus_tag="ACG8N9_002971"
     CDS             complement(<1..542)
                     /locus_tag="ACG8N9_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4898472.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTK"
     gene            877..>1112
                     /locus_tag="ACG8N9_002972"
     CDS             877..>1112
                     /locus_tag="ACG8N9_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898473.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVD"
BASE COUNT          408 a          207 c          169 g          328 t
ORIGIN      
        1 ttttttgtat tttcgtaata attatcgatt gccactgctt caaccttatg tttattgttt
       61 ttcttagatt gaaaattttg gatagctggt gcgacatctt tctccaaacc cacgataagg
      121 actttcttat cacgactgac tcttaagtac agtttgtaaa cgactatccg gtaaacacta
      181 agtaatatcg tacctaggat aaacgagatc aaaatgacgc cacgcggaaa tgcaaaccat
      241 cggccagcaa aagtaactac catgatcccc aatgctatta gtacttgtcc aataacagta
      301 atgaagacaa tatcactgat cattcggtta tagaatacat aagctcctaa caaaatattc
      361 aacgcaataa agatgatgga aataaaaatt gctgaatgct gaaaggtttc taagtttctc
      421 actggtactt ctccctggaa tttcagcaaa aaactaagat agatcgatac atttaataca
      481 agtacatcca taagaataat gaatattctg cgtgccgcat tccactcccc atttttattc
      541 atatatacac tcctaaccat aaaataatgc ctctatcatc ttaccatagc aaagtttaaa
      601 aacctagtga ataaccaaat tgtaaaggaa attaaaagaa acggggaaaa gcgtttattg
      661 tctgtttata tgtgtatttc tgcataacag aaagttgtct cagaagtccc atcacttcct
      721 ttatgctgaa ctagtattct cctgctaatt ccttattcac aagttacaat attcatataa
      781 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
      841 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
      901 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
      961 aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggc
     1021 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
     1081 gtcattcaat tacctgctca tgaagtagat ta
//
LOCUS       DAZUKD010000260         1106 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000260 DAZUKD010000000
VERSION     DAZUKD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.271"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            628..>1106
                     /locus_tag="ACG8N9_002973"
     CDS             628..>1106
                     /locus_tag="ACG8N9_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS4898474.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVA"
BASE COUNT          395 a          190 c          213 g          308 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctactaatt gagagtataa aatattttgt
      541 gtaaatgaaa aaatccatac aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa
      601 ccaattcaca gaaaagagga cttccctatg aatgatttta ctacagaaat tgtgcaaact
      661 ctagtcacta aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac
      721 acactcctac ggactgaatt aacggctttt ttagattacg aaaaatatga tcgcactggt
      781 tttaattcag gtaattcgag aaacggttct tactttcgat caatcaaaac cgaatatggt
      841 gaattaacat tggaaatacc tagagatcgt aatggtgagt ttaaacaaca aactttacca
      901 gcctacaaaa gaacaaacga tacattggaa accactatta tccatttatt cgaaaaaggt
      961 gttacgatgt ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca
     1021 caaaccatgt ccaacatgac taaagttctg actgaagaag taaatgcctt taaatccaga
     1081 gccttaaatg ataagtatgt cgctat
//
LOCUS       DAZUKD010000261         1103 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000261 DAZUKD010000000
VERSION     DAZUKD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.272"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..490
                     /locus_tag="ACG8N9_002974"
     CDS             149..490
                     /locus_tag="ACG8N9_002974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898475.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            858..1049
                     /locus_tag="ACG8N9_002975"
                     /pseudo
     CDS             858..1049
                     /locus_tag="ACG8N9_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          409 a          120 c          181 g          393 t
ORIGIN      
        1 ttttaatttt cagtttctat taaaaaataa tttttatggt ttattgaaat aaggttgcga
       61 aggaagatgt agagttattt gcactgttgt gttggtatat ctcttaatgc tgtgtctttt
      121 tggggagatt aattagtgga gtgattatat gagtatagat gattgtttag aagaacaaat
      181 aagattattt ttaaagtttt taaaagttaa agttcaaaat aactacttca catgttatag
      241 ttatgcacac cctgttttaa atagtcttcc ttttaattct ttagtgcaag gattgattac
      301 aaatattgtt ttattagtct gtacaccaaa tgaaatattt gtaaaaaaga taggcagtgg
      361 tatttcttca gtaagattaa aagagaaaga atatactcaa aagattattc atattcctaa
      421 gcctgaaatt aagcgttttg agataaaaga ctattctgct ttagggctgt tctcaattgg
      481 ttactggtga taaaaaataa gaatactaaa actatacgta gaatataagt aatcggcatc
      541 aatcctaatt atgtgattga atgccgattt ttgttacgcg ctatttttaa ttatctgtaa
      601 gaagaataat tttattttac tcatattcgt tttcactttg aagaagtaaa ttgcaatttc
      661 ttataagtta agcagctatg caaaaggcta tttattctat tgtgaaatat aagatgtagt
      721 tgaaacagga tatatagaat caaaatacga atcttatttt gatttttcta gttttttgaa
      781 aaaagcaccc tttgatcgtt gtattagtgg aaaagagaaa aaggagcata cttatcatgt
      841 cacaaaacta caactaaata tctaaacaac accaatttga tgcctattga aaaaaagtat
      901 tgcgaaatga agccaagtct attagaaaat gaaataatag aataaaagaa aatgggaaac
      961 cgctgaatga tgtaaatgaa ggtaagtatc cacgaactaa ttttgatgaa caagatatct
     1021 attttttatt tgtattggag atactagttt aatatcaaaa gttatcagca gtcataatta
     1081 gttatccaat gtaaaaagta caa
//
LOCUS       DAZUKD010000262         1097 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000262 DAZUKD010000000
VERSION     DAZUKD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.273"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1032
                     /locus_tag="ACG8N9_002976"
     CDS             <1..1032
                     /locus_tag="ACG8N9_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFS4898476.1"
                     /translation="TRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRK
                     LIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGT
                     THQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTD
                     RFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHY
                     HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLP
                     QSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIY
                     LQQGLIFEN"
BASE COUNT          337 a          240 c          211 g          309 t
ORIGIN      
        1 acccgttttc tctgccatga ttgtggaagg accttttctg cgaaaacgga cttagttgat
       61 gaacatcatc aactaacaaa ggaactgaag caggcaattc tcatggaact gtacgaaaat
      121 caatcacgta aactaatcgc taagaagtac ttcgtctcgg atgggacggt gacacggatc
      181 ttgcgtgaag caacgaagca ttaccagtca cgtatgaact tccttccgac ggtgttgtgt
      241 atggatgagt tcaaatcaat gaagtctgtt tctggttcca tgagttttat ttgtgttgat
      301 gggacaaccc atcagctatt tacgatcctt gaagatcgca gactttacaa actaactcaa
      361 tacttcctgc actttcctag aaaagcccgt ttaaaggtca agtacctagt gatggacatg
      421 aatgccagct attgtcagtt gcttaaaaca gtctttccaa atgcggagat cgtaactgac
      481 cgctttcata ttgtccaaca catcaatcgc agcttcaatc aattacgtgt ccagatcatg
      541 aatcgttttc gaacctcaca ctctgaaaat cagaagaagt accgtcggct caagcgatat
      601 tggaaactct tactcaaaga ttccacgaca tgggagccac tcaaacggca ttatcatcga
      661 ctgtttaaac gaccaattag tcaaacagaa atcgttgatg agctgttaag ctacaacgaa
      721 gaattgcgaa ctgcgtatca tttttgtcaa ttattacgct attatttcgt taaacgaggt
      781 acggaaggct ttcaagaaac ccttaaaaca atttcatcta cgttacctca gtcgtttaaa
      841 aagaaattca cgatcttcca ttgctaccaa tcgggtattt caaatgcctt taaggtgtcc
      901 cactcaaacg gcgtcactga ggggctgaat aacaagatca agctgattaa acgaattgcg
      961 tttggttatc gcaactttta taactttcgg gcacggatct atttacaaca aggtctgatc
     1021 tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat
     1081 tcaaatttgt cttcacc
//
LOCUS       DAZUKD010000263         1083 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000263 DAZUKD010000000
VERSION     DAZUKD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.274"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1015
                     /locus_tag="ACG8N9_002977"
     CDS             56..1015
                     /locus_tag="ACG8N9_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4898477.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT          397 a          187 c          200 g          299 t
ORIGIN      
        1 atttgtgcta cactcaaaat agttccgacg aaagaattat aaggagtgag tacaaatgac
       61 ctataaccat cttacaccaa ctgaactcgt aatgatagaa gcttatttca atcaatcaca
      121 acctgtttca aaagtagcca atctgttaca acgttctcga caaactattt ataaggtcta
      181 tcgttttctt aaatctggtg gaagcgcaat gagctactat aaacaatata agaaaaacaa
      241 acgtaactgc ggaagacatc cgattgtttt acctgatgag caacaggaat acattcaaaa
      301 aaaggttgtt caagggtggg ctcctgatgt ccttattggt cgagcagagt ttcctattgc
      361 ttgttcggta cgcacactgt atcgaatgtt taaagataaa acctttgaca ctcacgattt
      421 accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa aaaagaggaa aacaagggtt
      481 tcgccggtca atccgagaca gaaagacaga ctatacccag tttgatcatg aatttggtca
      541 cctggaaggt gacactatcg tgggtggaaa acataaaagc gcggtcatca cattagttga
      601 acggctatct aaagtcatca ttaccttgaa acccgaaggc agacaggcta aagacattga
      661 aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac ttatttaaat ccatcacatt
      721 tgattgtgga aaagaattct caaattggaa aaagattagt aataccaacg atatttcaat
      781 ttattttgcg gatccaggaa caccttctca acgaggattg aatgaacatt caaatggctt
      841 attacgtaaa gatggtttgt ataaagctat ggattttaat ggtgtatctg aaacctttat
      901 ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa tcattacact atcggacacc
      961 aatggaagta tttttgagtt acgtcacaaa agatgatttg tctaacttaa tttgacaaac
     1021 gaaaaataaa aaaattcaaa aaaagtattg cgttatttag aagaaattga tattatattc
     1081 atg
//
LOCUS       DAZUKD010000264         1065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000264 DAZUKD010000000
VERSION     DAZUKD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.275"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..569
                     /locus_tag="ACG8N9_002978"
     CDS             <1..569
                     /locus_tag="ACG8N9_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4898478.1"
                     /translation="VSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQE
                     NELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIA
                     NYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSH
                     FSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            586..1020
                     /locus_tag="ACG8N9_002979"
     CDS             586..1020
                     /locus_tag="ACG8N9_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898479.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          392 a          116 c          191 g          366 t
ORIGIN      
        1 gtgttagtgg agatacgatt cggtctgaaa aaaattattc tatcattgtt tttgaaaagt
       61 tagcacaaac gtctataaca ttaggaatgg atataataga agcgtatcaa agtagagatg
      121 ctttgataca agaaaacgaa ttagctgtat cgttacctga ggtcttaaaa gtgagggatt
      181 ctgggattgt ttattatact aaggaaatcg gaaaaactaa gatagagcat ctttctccct
      241 taatttcttc tgtcgttcaa ttcatcggct taaatatcta taaaagaatt actgtaaagg
      301 aaatagctaa ttatttttct gtaagtgaaa ctaaattaag agaaagcttt aagaatgaaa
      361 tgcatattac gatttataat tatatctcta agcgaaaaat ttctacggct aaaataatgt
      421 tgaaatcaaa ccatactatc agtgaagttt ctttaggttt aggattttca gattcttcac
      481 acttttcgag agtattcaaa aagtacgcag gtgtatctcc taagcaatat caattaggtt
      541 tagtggacaa tttcaacaac attagctgag aggttttatt ttattatgag actatatcat
      601 ttacgtagtt tggaggagtt gaggaaattg gattttaaca aaaaattttt ttgggaaatt
      661 tctgatttga gttgtttcgt agaatctgag cgtctaagta atggtaataa tagtgtgtta
      721 aatgaatatg acaaagtagt aattaataaa gtaaaaagtt tatgtgaaga agctagagaa
      781 tctatcagtc atattaatgg tagcaatttt aaacaaacat tatttaatct gattaagcta
      841 gatgctaaaa tatcaagtta tttattcttt ttgatacatg atgaatttat ggattatcaa
      901 aaattggatc agttaattga aagagaaagc tgggaagatt attacgtggg tctaactttt
      961 tcagaaaagt taaacaacta caagttaata gattataagt atttgagtga aagcaattaa
     1021 ttattcatgt agagtactag acactttaaa tatggacaat tcaat
//
LOCUS       DAZUKD010000265         1062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000265 DAZUKD010000000
VERSION     DAZUKD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.276"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACG8N9_002980"
     CDS             complement(37..993)
                     /locus_tag="ACG8N9_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898480.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZUKD010000266         1049 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000266 DAZUKD010000000
VERSION     DAZUKD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.277"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..848)
                     /locus_tag="ACG8N9_002981"
     CDS             complement(39..848)
                     /locus_tag="ACG8N9_002981"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4898481.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          319 a          201 c          183 g          346 t
ORIGIN      
        1 attgtcacaa cctctttttt gtggaatcta attctctatt atagtttaag tagtttttcc
       61 agataaacag ctaaaccgtc ttcctgattg ctcaaaggag tgacatcatt tgcaatgcct
      121 ttgagttggt cagtaccgtt agccatagct actccccaac ctgcataatc caacaattcc
      181 aaatcattat gctcgtcacc aaaggcaatg acgtcttttt gatcgataga caggtagtta
      241 gcaatctctt gcacgccttt tgctttttgg attccttttg aaacaatctc taagatggca
      301 gtaggtccac cccatgtccg aacatcgaca taactgccaa attgacgtgt taattcagca
      361 gatacagctc ctgcaaaacg tttgtctgtt cggacaagca aagatgtagg atttgtcgtc
      421 aaatttttag gagaaagcaa attcttctct cctggacgag aagaagcaaa aattttttca
      481 tcaaagaaat caaaactgtc aataaaaaaa gtatcacgat tttcagcagc gataaaatct
      541 aaatttagct gctttttttg cgataagatt tcaaaagcga tttcacgatt gatcaacgtt
      601 tctctttcac catcccagtg ctgattaggg atgtgtacta atgcgccgtt aaaattaacc
      661 atcggtgttg ttaattctaa ttgttggtaa aactgatggc tcattcggaa tgggcgtcct
      721 gtagcaatac tgacatgatg accggcattg attgcttttt ttagagtctc ttttgtttta
      781 gatgtaatca tcgaatcttg attgagggta gttccgtcca gatcgatggc aattaatttt
      841 ttattcaata agggtccttc cttcctcaac tagcatttgt tttagtctag catattttag
      901 gtaattaaca agagcaatca gctattccta acaaaaacta cagttatggc tatataaata
      961 gctaaacata agctaaacac gaacgttaag tattattttc gctttttttg ttaaaaaaac
     1021 gttgaaaagc gaatgaaacg tctatataa
//
LOCUS       DAZUKD010000267         1004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000267 DAZUKD010000000
VERSION     DAZUKD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.278"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..762)
                     /gene="aac(6')"
                     /locus_tag="ACG8N9_002982"
     CDS             complement(214..762)
                     /gene="aac(6')"
                     /locus_tag="ACG8N9_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4898482.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          298 a          209 c          147 g          350 t
ORIGIN      
        1 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
       61 aactaagcga ctttttcaca acctctggta cacggtgttc caaaagtagc ttcttcggaa
      121 ataagttgga atttcacaaa aattcaagaa gtaattttcg taaattcctt cttatttctt
      181 gaagctgatt acttctgtca caacctcttt ttattattga gaatttggtc gaggaataat
      241 cgtttttgcc atccaaatat ccggtttgtc ccagccattt gcatttggta agacacctac
      301 gattttataa cctaattttt catagaattc atacggatgt tcacgaaggt tctggatgga
      361 agccacttta tcaaatgtat gctcatacag gtccgtttga cttaacgttg ttccatggtc
      421 taaatcatcc gtacctaaat aaatcgtgat tcctcctctg gaagctactt ctttttctaa
      481 gtaattgact aatcgagtac ctatttggtt ctttcgtcgg gagctttcta caactaatgg
      541 atgcaattcc caacctgtga taccgtattg agggattgca ccaataaatc ctactaactc
      601 atcttggtct accgctgcta ccgcgattcg ttctggattc atcatttctt ctacttcttc
      661 tgccgagctg tctccatatt cttccggcca agtcagtctc agtaaatcag aaagctgatc
      721 tttcaatact ggattattac ggtcaaattc actgattatc atacgtcttc ccccttgttc
      781 ttgcttattc taacagtcct tttgaaaaaa cgaaatgttc tcgctcaaaa atctttagct
      841 accaatcagt atctctattc tttcattatt tatcttactt ataaaataaa aaaattatac
      901 agaaacactt tattcaaaat aggaaagatc aggaaagcca tcaattggag gaaaaaactc
      961 tgtttgttca tcatgatata gattatcttt tgctttaaaa aaaa
//
LOCUS       DAZUKD010000268          992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000268 DAZUKD010000000
VERSION     DAZUKD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.279"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..550
                     /locus_tag="ACG8N9_002983"
     CDS             <1..550
                     /locus_tag="ACG8N9_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4898483.1"
                     /translation="ADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRV
                     LSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESI
                     SQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKE
                     DLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          347 a          127 c          160 g          358 t
ORIGIN      
        1 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
       61 taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat ttaatttaaa
      121 caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc aaataagttt
      181 gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat cctcagtatc
      241 tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt cagagtcaga
      301 aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag gtttgtgtga
      361 tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg ttgacaaagg
      421 aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata tgaataaaga
      481 agacttagat agtcataagt taattagtta tccaattata gaaaaacaac aaattaaata
      541 tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac aaaaaggcaa
      601 agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt aatgttttta
      661 acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag tgtatattat
      721 gctatccttg tttggatttt aggacgttta aagaggcact tttttacata acttaatcca
      781 tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg caccccaaac
      841 cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca ctaagaagca
      901 taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta gagtaattaa
      961 ttagatgttt attttatagg actgatacta ta
//
LOCUS       DAZUKD010000269          978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000269 DAZUKD010000000
VERSION     DAZUKD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.280"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>978)
                     /locus_tag="ACG8N9_002984"
     CDS             complement(<1..>978)
                     /locus_tag="ACG8N9_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898484.1"
                     /translation="FPIYNIYLHFYKASYNYHLIDFMYKYPRSVLKDFAKEQFTSVST
                     VFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYYFYWNSTRESSDKWPFHIEQ
                     KEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSFRKVRVDNEYKSVISDDPHF
                     NLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYENSHAYAHQRQNTCSYRAVE
                     NLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNPDLFFNRSYIEEMKEEEPRT
                     YHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDYYDLFLTNVKP"
BASE COUNT          367 a          153 c          121 g          337 t
ORIGIN      
        1 atcggtttaa catttgttaa aaataaatca taataatcta acacataata ataatccaaa
       61 aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt tgcttgtaat
      121 tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt catttcttcg
      181 atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact tctttcatga
      241 aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa acggaacatg
      301 ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt ttgtcgctga
      361 tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc aaaagaataa
      421 attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat ttgttttgac
      481 cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata ttcattatct
      541 actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa agaaaataca
      601 cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac tatatacttc
      661 tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg tgtagaattc
      721 caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa ttctaactga
      781 aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt cgcatatctg
      841 aaaacagtag atacactagt aaattgctct ttagcaaaat cttttagaac acttctagga
      901 tatttataca taaaatcaat caaatgataa ttataagaag ctttataaaa atgaagataa
      961 atattataaa taggaaaa
//
LOCUS       DAZUKD010000270          977 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000270 DAZUKD010000000
VERSION     DAZUKD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.281"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            764..>977
                     /locus_tag="ACG8N9_002985"
     CDS             764..>977
                     /locus_tag="ACG8N9_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4898485.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDI"
BASE COUNT          358 a          159 c          121 g          339 t
ORIGIN      
        1 taaaaaccac gataggtaac tttaaacaaa catgaagaga ctttaataag acttctttat
       61 ctgaaaacac ttttgtttct ctctttcctt tctacatcat taaatagtat tttatacgtt
      121 atctaaaata ctattcctct gtcaaaataa gctaacttac ttcattataa aatatccact
      181 tatcgctttc atgtatattc caccgtaaaa agtacatttt atcaccgaga actcatcatt
      241 tttcatcatg atgaatacta tttacatata tctagttcaa agacaataat tattatgatc
      301 cgttattatc taagcactat acagataata ggaatttttc gtttctaaat attccaattt
      361 gggaatccaa taaaaatttt cgttcaaata cgtgtctatt cgaactcttt tctagttgtt
      421 aattatgaag ggggaagaaa taaaaaatac aatctataca cgaataagaa tattatccaa
      481 aaggaggttt tagaaaagat ttattatacg taaaaagctc aagacatttt cttagccata
      541 aggacatgtt tgatatttaa gaagatatat gaattttctt ttctaagtat aaagataact
      601 atccattatc aactaaaaaa acatttttga aaataaacat cagcagtcct atcaatgcat
      661 gtgagcttat agaagttatt cgcagcgcag cataattgca ttagcttctt ttcgtgtttt
      721 ttgcctcttt tccctttaaa tcttaatgga ggaggtgtgc aatatgtcag ataacgacga
      781 acgcgtaaat gcccagttga ttcgattctt tgaaaaggtc attttgaaca ccgctaatac
      841 ttattataaa aaacaagaag aaattcaaaa acatgaacaa cttgatcaac tcccaccata
      901 tttgtttact aaagatacta taaaatttaa acaaccgatt aatgttttaa atgtgactct
      961 ccttgttgaa gatataa
//
LOCUS       DAZUKD010000271          931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000271 DAZUKD010000000
VERSION     DAZUKD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.282"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..927
                     /locus_tag="ACG8N9_002986"
     CDS             130..927
                     /locus_tag="ACG8N9_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4898486.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT          395 a          130 c          126 g          280 t
ORIGIN      
        1 gtaaaaaaat gtatttattt gtatttataa gaataaagta tcatactcca gataaaaagt
       61 aaatactttt aaatactttt attttataaa tatgatatac tatctttaaa aataaaaagg
      121 agaaaaaaga tgaagccaaa tccttcagaa gcaggaaaac gaatcaaaca attacgcctt
      181 tcatgtggtt ttactatgga agaacttgga aggaaaatag ataattcacc acgtgcgacc
      241 atatccaact gggaacgtgg gaccaattta ccaaatccac aaaagctgaa attattatca
      301 actatcacta attcaacaat tgactggatt aaatggggaa ctttagaaga atatataact
      361 tcatacctta tcgatatagg gtatgagcta tatattaagg acttcccaga aatacctcac
      421 aaagtcttta aagacattca agaaagatat agtgatacgt tttcgctaga taaagactat
      481 gagttattga atcctataat aaaaaatata tttacaaaat attactcaaa agattttgaa
      541 atttatctaa atgagatact tgctaataat atcaatcctc tgattgaaga gtacgctaac
      601 caaagacact ctatctctaa agagctattt ttcaaaagag ccaacagtaa acttcaagac
      661 aagataagaa atggtgattt caaatacggt gaaaaagaaa gaatgattga ttctttaaaa
      721 cagctattag acgaaatgga cttagcctat aaagatattt caaagtattc atctgtcgag
      781 gatttctttt ataaaattac taaaaaccaa tttgaaacag aaaaatttat ttcagatctt
      841 agtaaaaaat acaatttccc atatgagaaa aatagtataa tcgcaaaatt tttaactgat
      901 aatcacgata aatttaaaaa taattgattc t
//
LOCUS       DAZUKD010000272          926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000272 DAZUKD010000000
VERSION     DAZUKD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.283"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..872
                     /locus_tag="ACG8N9_002987"
     CDS             111..872
                     /locus_tag="ACG8N9_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4898487.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
BASE COUNT          253 a          154 c          187 g          332 t
ORIGIN      
        1 taatatgcac atataaataa taagatgata taatcaacaa agaaaatgtg atttttttca
       61 ctatatttta tcacatgaaa attcattaag aaagtaggaa tcgaagtatt atgcacacac
      121 cagaagaaat tatggaactc agtattcatc ttggaacaaa aaaaatacat aaacctatct
      181 tagctaaact gattttaggt tttatcggag gagccatgat ttctctaggt tatttggcat
      241 acgtacgagt ctctgcttct atcccagaat ctatggctag cttgactagt ttagttggtg
      301 cctcggtttt tcctatcggc ctgctggtca tccttcttgg cggtggtgaa ttgatcaccg
      361 gaaatatgat ggctgtggcg attgccttta tggataaaaa agtcacattc tcacagctgg
      421 ttaagaattg gtcagtgatt acttttgcaa atattattgg tgcaattttc gttgcctatt
      481 tctttggaca ctttgttggt ttaactcatt caggtgtcta tctcgaccaa gtgattcata
      541 ttgctgatca taaaatcgct gtttcttggt ggcaagcatt aggctccgga ataggttgta
      601 attggtttgt cggtttagcg ttatggttat catatggagc aaaagatgcg gccggcaaag
      661 tactaacgat ttggtttcca gtcatgattt ttgtggcaat cggtttccag catagtgtgg
      721 ccaactgttt tgtgatacct gctgccatat ttgaaggtca agcgacttgg ttagaatttc
      781 tctcgaattt tatccccgtt tatattggaa atatcatcgg cggagctatt tttgtttcag
      841 gtttttatta cgttagttat aagcatcatt aagattgaag gtaatgttca ttcgagcatt
      901 accttcaatc tctttatata ctattt
//
LOCUS       DAZUKD010000273          922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000273 DAZUKD010000000
VERSION     DAZUKD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.284"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>922
                     /locus_tag="ACG8N9_002988"
     CDS             <1..>922
                     /locus_tag="ACG8N9_002988"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4898488.1"
                     /translation="FSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRG
                     KEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTS
                     GTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFF
                     TSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKT
                     ARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSM
                     FSLAIEEQITLDKLKMLDYLFHPSFSQPYNF"
BASE COUNT          346 a          115 c          156 g          305 t
ORIGIN      
        1 ttttcgataa agaattttcc aaatgcaaaa gagattttta aagctttgga tgatagtact
       61 attaaaaaag taggaattat tggagcagga tatattggag ttgaaattgc tgaagccata
      121 agaaaaagag ggaaagaagt atatcttttc gatgttgcag atagagtgtt gtcgacttat
      181 tacgaccgta gtttttcaga taaagttgaa gaaatactat caaaaaatgg aatacatctt
      241 tgtctatcag aaagtcctaa aaaatattta ggaagaaaaa agattgaaaa aatagtcact
      301 acaagtggta cccaatactc tatggatatg gtcgtacaag ctataggatt tctatctaat
      361 agtccaattg gggaaaagga actcttgaga gatgttagtg gagcatatct aactaatgaa
      421 taccagcaaa caaatataaa agatatatat gctattgggg attgtgctac aacttttttt
      481 acgtccatta atagaagtag tgttgatttt tctatttcta atgctctaag aacagcttat
      541 attgcaactc aacatattaa caatcaagat tttacacctt ctggttccca atttacaaat
      601 ggcttttcta tatttaatta caatttttac gcagcaggtt tgaatttaaa aacagcacga
      661 ttattaagtg agtctataga ttatatagaa atagaagatt tcattagacc tgcttttttg
      721 agaaaaaata gtcgtgtaaa attaagaatt agctttgaaa aaaatacaaa gagaatagta
      781 ggtgtacaac tttgtagtca agaagattta tctggactat tatcaatgtt ttctcttgct
      841 atagaagaac aaattacatt agacaaattg aaaatgttag attatttatt tcatcctagt
      901 tttagccaac cctataattt ta
//
LOCUS       DAZUKD010000274          916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000274 DAZUKD010000000
VERSION     DAZUKD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.285"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(260..>916)
                     /locus_tag="ACG8N9_002989"
     CDS             complement(260..>916)
                     /locus_tag="ACG8N9_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898489.1"
                     /translation="RGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHI
                     MDGDYKLNQSNLIFDGRKLFFNLTISFEKRYNELEFKEGNELIVNFADLCSGECLVNN
                     KSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNF
                     VKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS
                     "
BASE COUNT          276 a          180 c          108 g          352 t
ORIGIN      
        1 ttatatggtt ctaaagcagg aacgccatca attaatttaa agtactcatt tgagaaacat
       61 gctgttttat atggctctaa aactcttatt ataacataaa tgaaacaaac acaatttgag
      121 aaacatgctg ttttatatgg ttctaaaacc ccaacaacgc tcgtccttcc gtctggtcac
      181 acaacttgac ggagtttacc cttcagtgag tggattttcc ctcactattt cgttgccaaa
      241 tcaacgattg tttactttac taacttctga cttctatccc atatcttttt cctttattta
      301 ctagttgttc tttcaattga tagtaagccc attcgccata agccttatct ggttcaatga
      361 tacgaataat ttgagcttta ttgcgaatag cagttttaac gatttttgca gaacattgat
      421 tattataagt tttaacaaaa ttactttctc ttttctttat tgcttcccat cgatcttgat
      481 ctaattttct ttttcgacca tgaccgcctt tagaaaagac tcctcggctt tgttcttttg
      541 atttcattgc taaaatttta cttttgaagt ttcttatata ttggctatca ccaaaagaca
      601 ttaccgattt attattaact aaacattcac cgctacacaa atctgcaaag tttacgatca
      661 attcattacc ttccttgaac tccagttcat tatatctttt ttcaaaagaa attgttagat
      721 taaaaaataa ttttcttcca tcaaaaataa gattactttg atttaatttg taatcgccat
      781 ccataatgtg ttgtaaaact gaagcaacat ctcttgcttt tcttggcttg ctacctaaat
      841 tcactttaaa cctaaagcct ttaggtaaat aaatataaaa cttatcatct ttatggattt
      901 taatatctcg acctct
//
LOCUS       DAZUKD010000275          904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000275 DAZUKD010000000
VERSION     DAZUKD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.286"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..871
                     /locus_tag="ACG8N9_002990"
     CDS             170..871
                     /locus_tag="ACG8N9_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4898490.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          326 a          159 c          146 g          273 t
ORIGIN      
        1 cattcgcttc aaaacaaatt attcaatacc attgtatatt ttttacataa taacaaatta
       61 tatttgtaaa gtattaaaac ttgtttctaa tgttggtatg ttaaaacttt acaaattctt
      121 taaaaaaagt gacaataaat atagtgaaca ataaggagag gactttttaa tgcgtgttaa
      181 taaaatcgat tttcactttt caaaactcag atatcaacct gattttgaac atttctcgga
      241 tgaagagttc caacagatca aggaacatac tgttcttcgt tcttataaaa aaggacagat
      301 ccttttcgac gatggtgatc ccagacaacg gttgtatatc ttgaccaaag gactcgttcg
      361 tttagaacgt tatgatgaaa gtgcaactta ttactactat gactatgtga atacaaataa
      421 cctttttcct atgtcaggaa tgtttggaga agagacctac acttatacag ctcaagcgat
      481 gactgatatt gaaacattct accttcctgt ttcctactat gaaaaattag cacgaaaaaa
      541 tcccgaacag ttgatctata tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt
      601 gcgtatccaa atcggattga cctctagcgc ctttgaccgg gtaaaacaaa atttattcat
      661 tttgaaagaa gaattgggcg taccaacaaa agatggtcat atcaccattc cttatccgat
      721 caccttaaaa gaattagcag tgaatagcgg cacgacacgt gaaactgctg gacaagtaat
      781 caaacaacta cgtgattccg gactgttaga ttatgacaaa aagcgtttta ctttctgtaa
      841 gacagctact actccagcta tgagagaata acgcgaacaa aaataagcag tgaccctgat
      901 attt
//
LOCUS       DAZUKD010000276          899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000276 DAZUKD010000000
VERSION     DAZUKD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.287"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>899
                     /locus_tag="ACG8N9_002991"
     CDS             <1..>899
                     /locus_tag="ACG8N9_002991"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4898491.1"
                     /translation="QALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLV
                     PMSEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNG
                     AKVPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGS
                     RLDDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEF
                     ILQFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDT
                     IALHQRKLDLLKETKKGFLQKMF"
BASE COUNT          344 a          145 c          167 g          243 t
ORIGIN      
        1 atcaggcttt gattagactt agaataaata aagaaatcac agattcagaa tattttattc
       61 aatttattcg tgcagatttt atgcaaagaa aactcacagg agctaatcca ggttcagcaa
      121 ttacaaatct cgtaccaatg tcagaagtga aaaaatggat agtacaattt ccaatattag
      181 aagaacaaaa gaaaattggg aacttcttca aacaactgga cgacactatc gctcttcaac
      241 aacgtaagct agatttatta aaggaaacga aaaaaggatt cttacaaaaa atgttcccta
      301 aaaacggagc aaaagtgcca gaaattcggt ttcctggatt tacggaagat tgggaacagc
      361 gtaagttcaa agagtttagt aagaagactg gaaaaaagaa cacgaaagat ttagacttcc
      421 ctgcctattc ggtaagtaat aaagcaggtt taatttctca aacagaacag tttgatggta
      481 gtaggttgga tgatttagaa aaaacaaatt ataaattcgt tgaaccaaat gaattcgcct
      541 ataatccagc aagagtaaac gtaggatcaa ttgcatttaa caatcttggt atgacggtca
      601 ttgttagctc tctttatgta gttgtgaaaa tgagcgagga cttagataat gagttcatac
      661 ttcagtttat caagtcacca acatttatca aagaggtcaa gcgcaacact gaaggaagcg
      721 tacgagaata cttattctat gagaatttcg ctaatatcaa gtttcctttt actaggaata
      781 aagaggaaca acaaaaaatc ggcgctttct tcaaacaact cgacgacacg atcgctcttc
      841 atcaacgtaa gttagatttg ctgaaggaaa cgaaaaaagg cttcttacaa aagatgttt
//
LOCUS       DAZUKD010000277          890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000277 DAZUKD010000000
VERSION     DAZUKD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.289"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8N9_002992"
     CDS             <1..888
                     /locus_tag="ACG8N9_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4898492.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZUKD010000278          880 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000278 DAZUKD010000000
VERSION     DAZUKD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.290"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(321..494)
                     /locus_tag="ACG8N9_002993"
     CDS             complement(321..494)
                     /locus_tag="ACG8N9_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898493.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHLFIFAYILAMIA
                     FLSGGWLIIYLLS"
     gene            complement(497..>880)
                     /locus_tag="ACG8N9_002994"
     CDS             complement(497..>880)
                     /locus_tag="ACG8N9_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898494.1"
                     /translation="NKNYTTIILEDFTDQELLIVGKTKADFIKKGNASIVKASKVAVE
                     KYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHTSMKPC
                     IFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT          322 a          146 c          100 g          312 t
ORIGIN      
        1 ggatcatatt aatctgaata caatatatga actaataaat agcttaacct cgctattttg
       61 ttttttctga tcattatcta cacagcatta acaaatagaa ttttaaaaat aaaaaaagta
      121 agacaaaaaa agtatcaaca taaacatata catatacacg tttatgttga tacacttaat
      181 ttgtccttta aaaagcactg atttttaaat tgcgttattt tataacccga atacagtctg
      241 cttgttaggc attgtcctta aattacattt ttcataggtt atttaatcat ttaatctgtt
      301 gattattctt gatctaaaac ttagcttaac aaatatataa tcagccaacc gcctgataaa
      361 aatgcgatca ttgctaatat ataagcaaag ataaaaagat gttcgatcag cttaaaccct
      421 gatgcgatta agcagaccaa gtaaaataga aattctctta ctgataatgg taattttcta
      481 actagcatac tcataattaa caccccaaag taatattgta ttttgaacat tttacatcca
      541 gtacagcatc aatcaaccat tgccaatcaa aaatacaagg cttcattgaa gtatgttttt
      601 cagatgaaaa gatataatat aattcctttg gaataatatt ttcagttgtt gggatcaagc
      661 taaatctaat tccatctaac tcatagctac cagctttctc tccattaaaa atttccttag
      721 cactttcttc taaagaatca aaatattttt cgacagcaac ctttgaagct tttactattg
      781 aagcatttcc ctttttgata aaatctgctt ttgttttacc gacaataagt aattcttgat
      841 cagtaaaatc ttctaaaata atggtagtgt aattcttatt
//
LOCUS       DAZUKD010000279          859 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000279 DAZUKD010000000
VERSION     DAZUKD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.291"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            325..723
                     /gene="spxA"
                     /locus_tag="ACG8N9_002995"
     CDS             325..723
                     /gene="spxA"
                     /locus_tag="ACG8N9_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4898495.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          312 a          117 c          157 g          273 t
ORIGIN      
        1 ttaaagaaat aaaaaacata aaaaatctta agaaatcggt ttcatttagc ttttgagata
       61 aaataatagt acagttttat acgctgtttc atatgtttag aaaatttgaa ttagatttga
      121 aaactctaat ttttaaatga taaaaaaagc gagaatgctg gttttactgt atattaacct
      181 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
      241 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
      301 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
      361 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
      421 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
      481 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
      541 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
      601 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
      661 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
      721 tagaaggata atacgaaact tggaaaagac tggctctttg tcaaatgatg ttggtaaaga
      781 gaaatcttag aataatttgg acagaacaga aagaagaaaa cctctttctg ttctgtttat
      841 ttggttaaaa atgttttgc
//
LOCUS       DAZUKD010000280          832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000280 DAZUKD010000000
VERSION     DAZUKD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.292"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            404..718
                     /locus_tag="ACG8N9_002996"
     CDS             404..718
                     /locus_tag="ACG8N9_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898496.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT          329 a           97 c          148 g          258 t
ORIGIN      
        1 gatgaattta gtggctagca tatcgtgtga ttaagatact ttatagtgta agttgaagga
       61 gaaaaaatat ttaaaatata cttcccattt cagtgggaag taaaattaat cttgttgttt
      121 ctactagtaa tgtatctttt tggaaactat gtaacgatgt aaacaagatt tttttcagaa
      181 gaaaaaacga taaaagttgt gataaacgat ttaaaaaata ggagaaaata tatcaagcgc
      241 atggtatgaa acgcacattt aaaaagattc gactaactaa aaaaataagt ttacaaagca
      301 ttgttgtgta ttacaagtga tctatacact gaaaaatgat agagaaaatc tgatacgtcc
      361 gcaacaatgg acaattgaaa atgaaagtga ggaaattgga agaatggctg atgaaaataa
      421 agttgttccg cttgaaacac tagtgataaa taaggaagag tttgagcaac tttttcttga
      481 tttttttgtt caatatgaca ggatttatga ttttagttcg tcactagatt tatttgaatt
      541 agaggcagaa ataatagaaa acagtcgaga ggagaatggt aaaatcatca tttcagtaga
      601 aaaattcaat gaattattct cagaaacatt tgaaatagat acagattatt atcaaccaca
      661 agaagttttc caatcattta gaaatcagat tattcgctca ggtaagaccg taaactgaaa
      721 aacaataaaa gaaacgttga aagaataatt attccttcaa cgtttctttt ttagaatgtt
      781 ttaatgacca aagcagtttc attgtcaggc ttaggtaatt catgaaatcc cg
//
LOCUS       DAZUKD010000281          823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000281 DAZUKD010000000
VERSION     DAZUKD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.293"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..>823
                     /locus_tag="ACG8N9_002997"
     CDS             14..>823
                     /locus_tag="ACG8N9_002997"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4898497.1"
                     /translation="MNPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYGGLACRLRK
                     VSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQS
                     KYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCV
                     LDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLE
                     SAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYEQR"
BASE COUNT          314 a           95 c          158 g          256 t
ORIGIN      
        1 agatagaaga ggaattaatc caaaaactga ctttaaacta ttttttaact attgtaatat
       61 tttgaaaaaa ataaagccag atatggtcat tgcttatact atcaaaccaa atatatacgg
      121 tggattggca tgtcggttaa gaaaggtttc ttatgcatta aatataaccg gattaggaac
      181 ggcatttcaa aaaaaggggg ttttgaaaag tctcattgta tttttgtata gattagcagc
      241 taaaaaatct aaggtcatat ttttcgaaaa ttttgaaaat atgaaaacat ttcagacttt
      301 acaaattgct aaacaaagta aatacaagct attaaacggt gcaggagtag atgtagaaca
      361 ctttagttat ttagaatatc ctaaagatga gcaagttcat tttttattta ttggaagaat
      421 tatgaaagag aaaggtgtag atgaactttt tgaagcaatg aaaaacttat atagtgaaga
      481 gaaaaattgt gtacttgatg tccttgggta ttttgaagaa gattacggca taaaaattga
      541 aaaatacgaa aaagagggat ggttaaaata tcatggatat caatctgata ttcgtccctt
      601 tgtagaaaga gcacattgct ttgttttgcc tagttggcac gaaggaatgg caaatactaa
      661 ccttgaatca gcagcatcag ggagaccaat tattacaagt gatatcccag ggtgtaaaga
      721 agcagttatt gaatcgaagt ctggttattt atgtgaaaga aaaaatagtt gtagtctcta
      781 taattcgatg aataaatttc taaaattatc atatgagcag cgc
//
LOCUS       DAZUKD010000282          783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000282 DAZUKD010000000
VERSION     DAZUKD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.294"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..311
                     /locus_tag="ACG8N9_002998"
     CDS             117..311
                     /locus_tag="ACG8N9_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898498.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            580..>783
                     /locus_tag="ACG8N9_002999"
     CDS             580..>783
                     /locus_tag="ACG8N9_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898499.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIA"
BASE COUNT          329 a          101 c          129 g          224 t
ORIGIN      
        1 tgaaaaaaac tttcaaattg attcttttta tttatcataa agatatagat acataatcat
       61 tttcacttac atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt
      121 attatgtaga agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta
      181 acgaatatcc tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca
      241 taaaaataaa gaacgagcta gaaaaagaac tgacttgtgg caaagctgtt gtagatatca
      301 tcgaaaagta aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt
      361 gctaagatta tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg
      421 tttgtatacc atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat
      481 atcaagatga atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt
      541 ttatttttat aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt
      601 gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt
      661 tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact
      721 cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata
      781 gca
//
LOCUS       DAZUKD010000283          781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000283 DAZUKD010000000
VERSION     DAZUKD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.295"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..608
                     /locus_tag="ACG8N9_003000"
     CDS             207..608
                     /locus_tag="ACG8N9_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4898500.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            602..>781
                     /locus_tag="ACG8N9_003001"
     CDS             602..>781
                     /locus_tag="ACG8N9_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898501.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVD"
BASE COUNT          264 a          125 c          151 g          241 t
ORIGIN      
        1 acttggcagc cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag
       61 gaacaatctc atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata
      121 tcttgaggtg catttgttag taaggtactg ttctatgggg cgcttacgtt gtattagtgg
      181 aaagggggaa aaaggagcat attatcatgc aacaacatta caatgacata tctatacaac
      241 accaatttga tgcctactgt aaaaaagtat tacgaaacga agctaagtct attcgaaaac
      301 gaaacgctaa cataaaggat aatgaggaac cattggcaga ttcaaatgaa ggcaggtatt
      361 cccaaactca ttttgatgaa caagatgtct atttcttatt tggaatggaa atattaattt
      421 cagatcaaaa gttatcagca gcaattgatc agttatcgga gataagaaga aagattatcc
      481 tactttatta ttttgcaggt tttaatgatg ctgaaatcgg taaaattctc aatatgagca
      541 caagtggtat ctggtatcaa cgaaagaagg cagttgaaca attgaaaatg gggtatgacc
      601 tatggtagat aaatctaaaa agcggcagta cctattagtt ccactatcca ttatcttatc
      661 tgctctagaa ggagatccaa tttcgatcaa gatgatactc aaacattatc attcttatat
      721 ggctaagtta tgtttgacaa atggatttaa cgaggtaggt caattcgtta cctatgtaga
      781 t
//
LOCUS       DAZUKD010000284          772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000284 DAZUKD010000000
VERSION     DAZUKD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.296"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>772)
                     /locus_tag="ACG8N9_003002"
     CDS             complement(109..>772)
                     /locus_tag="ACG8N9_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS4898502.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          213 a          178 c          149 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gc
//
LOCUS       DAZUKD010000285          704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000285 DAZUKD010000000
VERSION     DAZUKD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.297"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..636)
                     /locus_tag="ACG8N9_003003"
     CDS             complement(61..636)
                     /locus_tag="ACG8N9_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4898503.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          235 a          151 c          125 g          193 t
ORIGIN      
        1 ataataaaat gaaaaaacag agccagatta gcattatttt gctaatccag ctctgtttat
       61 tcagcctatt tttctttttg aatatatcaa gcgttggggc aacatagtga tgactaatga
      121 aacaacgacg atagacaaca agcgatcaag gtagtctgta cccgcctgta ccaagatcac
      181 acttgttgtc tgacttaaac ctaacccatg taatagctga acgatcatac tggatcctga
      241 ggaggtgatc ccgccaaata actgaacggt aatgatcgaa gaaacgatcg ttccaggtac
      301 cgacaaaatc aaagcgagta ataacagttt caagctgttt ttatctttgc cgagatatct
      361 tggcaaaatc aatccagcaa gcaagcctgt gagaatttgg attggcgaat aatacagaga
      421 aaaaatatct gtagtcactc cacttaaaat accgctggac aatcctgtca gcattccagg
      481 tacaggccca agcaatgcac cagcaaaaat cgttccgata gaatcgagat agattggcaa
      541 acgaagccat aaagcaatcg ttcctccaat ataattgatc ccaatacaca aagccatcac
      601 tgtaagtacg cgagttgcca ttttgttttt attcattttg ttagcccatc tttaaccttt
      661 ccaaatcttt cataatcgtt tcttcttgtg catttaaaag aacg
//
LOCUS       DAZUKD010000286          704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000286 DAZUKD010000000
VERSION     DAZUKD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.298"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            451..>704
                     /locus_tag="ACG8N9_003004"
     CDS             451..>704
                     /locus_tag="ACG8N9_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898504.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVR"
BASE COUNT          255 a          105 c          120 g          224 t
ORIGIN      
        1 tgttgctgaa gattagaatg acaaaactat ttgaaactac gcgtatagta agtagctttc
       61 aaaatatata aaaaaggagc gttctcaaat gagaacatgc tcctaaaact actttattgg
      121 cgtatggaaa aattatactt gaagtggata gcgggttgaa taagttgata agcatcaaaa
      181 catttataaa aattattcaa gactatgtct gttccatata caaatatgaa aatcagtaaa
      241 ctccatttaa cttttttcgt agtgtgtctt tttatacatg tttatcgtta ttaatagtga
      301 ataggaaaaa aactattttt gatcattgac aattaaatac aatttttgaa taacgatccc
      361 tgctattcga gctagaaact caagtacgcc atgacctgat tagcgacaaa tacttagaga
      421 tttctttttg ttctgggaaa acaaaatgcc atgacaatag cagcaaataa tgatacttcc
      481 gtaaggctcg ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcaattc
      541 aaaaatttca taactactat tttaggagat gattttatag aatccagagt ctacctgaat
      601 aaaaaacaaa cctgtgcatt tctaggaatc gataataata ctctagataa gtggattcgt
      661 atatataatt tcccagtaat aagagttggt ggtgtagttc gctt
//
LOCUS       DAZUKD010000287          697 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000287 DAZUKD010000000
VERSION     DAZUKD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.299"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..453
                     /locus_tag="ACG8N9_003005"
     CDS             85..453
                     /locus_tag="ACG8N9_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4898505.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          245 a          115 c          107 g          230 t
ORIGIN      
        1 ttacttgtgt tttgttcgtt ttttatacat aactattgtt tttctggtaa aataaatcaa
       61 accacttaat ggaggcgttt tagaatggaa aaatttcagc cgatcatcaa tcagatcaaa
      121 gaaaaagaac atcaacaaaa gatcaaagac ctttttgcta agataacaga aacgtatccc
      181 cagctagaca caactgtaaa atggaaccaa cctatgttta ctgaccatgg tacctttatt
      241 attgcattta gttttgctaa aaatcatttt tctatcgcac cggaaaaagc agcgattcgt
      301 gcccttgaaa aaaatatcca agaagcaggc tacgtatata cggataacgt catcaaagtt
      361 ccttggaaaa gtgtaatcaa ctgggaattg attgaacaat tgatttcatt taatattgaa
      421 gataaaaaag gtcatgataa attttggcga taaagcttta tacgaataaa ccttttactg
      481 gcgaggaaac tattcatcag cttatacagg ttttgttcaa tcctatcctt tttatcatcc
      541 ctggttaaac acttaattga caaagtatct gctattttta taaggagtct ttcttgtgct
      601 gaaaaaaatc actgattgaa tggctttgaa ttttccactt agtaaaatta ttttttcagc
      661 aattttcctg caaaactttt gaataaggaa aaaatac
//
LOCUS       DAZUKD010000288          640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000288 DAZUKD010000000
VERSION     DAZUKD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.301"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..283
                     /locus_tag="ACG8N9_003006"
     CDS             <1..283
                     /locus_tag="ACG8N9_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4898506.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DAZUKD010000289          638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000289 DAZUKD010000000
VERSION     DAZUKD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.302"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>638
                     /locus_tag="ACG8N9_003007"
     CDS             <1..>638
                     /locus_tag="ACG8N9_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFS4898507.1"
                     /translation="TAVGIYSNYSLIVTSVQNLCKQITNSITASVGNFAVSNKKKDSF
                     ELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQVYRN
                     TSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGFVIW
                     YEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGGSTFLQ"
BASE COUNT          198 a           84 c          105 g          251 t
ORIGIN      
        1 taacagctgt aggaatttat tctaactatt cattgattgt cacaagtgtg caaaatttat
       61 gtaagcaaat tactaattct atcacggcaa gtgtaggaaa ttttgctgta tcaaataaaa
      121 aaaaggattc atttgagcta tttaaaaaac atttttttat caaccactca ctaatctttt
      181 tttcttctat attgttattg actttaatga atcccttcat tagcttgtgg gtaggagcca
      241 aatacatgct acctatgtca acgactattt taattgtttt aaattatagt gtacaggttt
      301 atagaaatac atccttcgtt tttatagagt catttggttt atattggtct caacggaaaa
      361 agcctataat tgaagcaggt gtaaacttgt tattatcttt gcttttgtta atagtttttg
      421 atatgggaat caatggagta cttataggga ctatcggatc gtctctagga tttgtgattt
      481 ggtatgaagc atttatcata tataaaacgg tatttgagaa acgttttatt catttctttt
      541 taactttctg gcgaagtttt atagaattaa taatttcggc aatagtcgtt tttctattaa
      601 atgaatattt agcaaatgga ggcagtacgt tccttcaa
//
LOCUS       DAZUKD010000290          630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000290 DAZUKD010000000
VERSION     DAZUKD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.303"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>630
                     /locus_tag="ACG8N9_003008"
     rRNA            <1..>630
                     /locus_tag="ACG8N9_003008"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DAZUKD010000291          596 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000291 DAZUKD010000000
VERSION     DAZUKD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.305"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..428
                     /locus_tag="ACG8N9_003009"
     CDS             <1..428
                     /locus_tag="ACG8N9_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS4898508.1"
                     /translation="YILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDR
                     ELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYII
                     KMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
BASE COUNT          239 a           62 c          117 g          178 t
ORIGIN      
        1 agtatatttt aggtgaagaa tatactatag atgtttgctt aaactttgat tcgacaccag
       61 taagtattgt tcctagaaaa agaatagcaa ctagaggtgg ggaaatactg aagggtcaga
      121 ttattaaaga tagagaacta attgagacta cgaaaaagat gttggaaaaa ttaaatgcaa
      181 tcggacatat tactattcaa tgtataaaaa acgaaaaggg aatattttat ttagaaataa
      241 atgcaagata cggtggagga gcccccatta gcattaaatc aggagctaat tcccctgaat
      301 atattataaa gatgatagat tcaaaaaaaa atttgagata cagagaaaat tatttagata
      361 aaatgatagc actgagatat gatcaagcga tttatttaga tgaaaaagga aatgtatata
      421 atgattaaag cggtgatctt tgatttagat gacactcttg tgtctgaata tgattatgta
      481 aaaagtggat acaaatgtgt agctagaaga ttagcagtgc aaaaaatttt cccggctact
      541 tttgacgaaa tatatagagt ccttttagat ctttttgctg aagatagtaa aaatgt
//
LOCUS       DAZUKD010000292          581 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000292 DAZUKD010000000
VERSION     DAZUKD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.306"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>581
                     /locus_tag="ACG8N9_003010"
     CDS             <1..>581
                     /locus_tag="ACG8N9_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4898509.1"
                     /translation="KVTLGWTPDVIIGRKERPVSCGMRTLYRLFSKGIFDIDTLPMKG
                     KRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERL
                     SKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDI
                     YFADPGTPSQRPLNENSNGILRRNGLPKSMDFR"
BASE COUNT          187 a          114 c          111 g          169 t
ORIGIN      
        1 gaaagtcact cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggcctgtttc
       61 atgcggtatg agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact
      121 accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta
      181 tcagcgctca atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca
      241 ccttgaaggt gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga
      301 aagattatct aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga
      361 aactgccctt aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt
      421 tgactgtgga aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat
      481 atattttgcg gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat
      541 tctgcgtcgt aatggactgc cgaaatcaat ggattttaga a
//
LOCUS       DAZUKD010000293          544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000293 DAZUKD010000000
VERSION     DAZUKD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.307"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8N9_003011"
     CDS             73..234
                     /locus_tag="ACG8N9_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4898510.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>544
                     /locus_tag="ACG8N9_003012"
     CDS             253..>544
                     /locus_tag="ACG8N9_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898511.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKV"
BASE COUNT          223 a           52 c           93 g          176 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtag
//
LOCUS       DAZUKD010000294          539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000294 DAZUKD010000000
VERSION     DAZUKD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.308"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>539
                     /locus_tag="ACG8N9_003013"
     CDS             <1..>539
                     /locus_tag="ACG8N9_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4898512.1"
                     /translation="VHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGK
                     KQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLK
                     ELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRER
                     IYEIGIFLSIGTSKIQIIM"
BASE COUNT          215 a           47 c           95 g          182 t
ORIGIN      
        1 tgttcatgaa gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga
       61 attactagat attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat
      121 cttttctggt aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat
      181 ggtgtttgta gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat
      241 tgcaaataaa attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa
      301 attgaaagag cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt
      361 tgaagagtct ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc
      421 tattatgtta ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag
      481 aatttatgaa ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgc
//
LOCUS       DAZUKD010000295          516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000295 DAZUKD010000000
VERSION     DAZUKD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.309"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..399)
                     /locus_tag="ACG8N9_003014"
     CDS             complement(277..399)
                     /locus_tag="ACG8N9_003014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898513.1"
                     /translation="MNMNRDVLKMNEFDQSLSYRNLQFRSIGLSINEKVKKNMF"
BASE COUNT          175 a           98 c           59 g          184 t
ORIGIN      
        1 taacacagtt atttttaaaa ataagaatgc taaatcactc tacaataacg gcgaaagcac
       61 aaaaaataaa aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga
      121 tctcattctt tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt
      181 atatattcgt attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg
      241 tccgttactt aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt
      301 ttcattaatt gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc
      361 aaactcattc attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac
      421 atcactttat tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat
      481 acactactat tcagcccatt caacgaccaa tgaatt
//
LOCUS       DAZUKD010000296          504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000296 DAZUKD010000000
VERSION     DAZUKD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.310"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>504
                     /locus_tag="ACG8N9_003015"
     CDS             <1..>504
                     /locus_tag="ACG8N9_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4898514.1"
                     /translation="IMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFM
                     LITAILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVT
                     PVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGI
                     TGYAQVYG"
BASE COUNT          163 a           99 c           92 g          150 t
ORIGIN      
        1 tattatgaat tttgaagatg aaagcatcat tgaagcttct gggttcagaa tccaaccaga
       61 atacgaacta ttcaagcgtc tctttgatat catcgtttcg cttattttac tgatcctcgc
      121 ctcacctttt atgctaatca cagcgatttt ggtaaaaaca acttcacctg gaccaatcat
      181 ctataaacaa gtacgtatta cgaaaaatca aaaggaattt tcaatctata aattccgttc
      241 catgagtgcg acagctgaag caaaatcagg accagtcttg gctaaaagta acgatgcacg
      301 tgtgacacca gtaggtaaat ttatccgtgc tgtacgtttt gacgaattac ctcaaatctt
      361 taatgtattg aaaggcgata tgtcgattgt cggtccaagg ccagaaagac catttttcgt
      421 tgatcagttc aatgaagaaa atccttatta ttacttaaga cacaatgtac gtgcgggaat
      481 taccggatat gcacaagtat acgg
//
LOCUS       DAZUKD010000297          490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000297 DAZUKD010000000
VERSION     DAZUKD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.311"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8N9_003016"
     CDS             <1..390
                     /locus_tag="ACG8N9_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4898515.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          187 a           85 c           75 g          143 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccaattagga ctcaattgtt catagatgag catttgtaat
      481 taagttatat
//
LOCUS       DAZUKD010000298          486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000298 DAZUKD010000000
VERSION     DAZUKD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.313"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..>486
                     /locus_tag="ACG8N9_003017"
     CDS             265..>486
                     /locus_tag="ACG8N9_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898516.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKK"
BASE COUNT          204 a           49 c           70 g          163 t
ORIGIN      
        1 tatttcagaa aaaaattaaa ctagattata ataattcttc atgtaaaaat aaaaacaatt
       61 acagctatga tgtttaagat aaaacaatta tgaaatcgat atccgtttat atacttatca
      121 taagctagaa gcaaataact agcctatgta tgtaagtgag attatattta ttaaaaggct
      181 tcttttgttc gatcagttat agtcgaacga gagaagttta ttctttgatt aaaataaaca
      241 aaagtaactt attgaggtga aatgatgcaa acttttttaa ctaaaacatc tttcaaaaaa
      301 gtagttttat tagattattt gttagaaaaa gacaattggt gtgcaatgga agaacttaga
      361 aatttactta atgttacgga gaagtcagtc ttacactata tagaagaact agaggattta
      421 tttaagcaat ataatggtaa tatccttctg aaaaatgaag ataataaacg atttttcata
      481 aaaaaa
//
LOCUS       DAZUKD010000299          448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000299 DAZUKD010000000
VERSION     DAZUKD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.315"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..365)
                     /locus_tag="ACG8N9_003018"
     CDS             complement(12..365)
                     /locus_tag="ACG8N9_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898517.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT          152 a           77 c           41 g          178 t
ORIGIN      
        1 aatagttcat attactcaat gtaaaaaata attgattgtg gacattgctc atccactttg
       61 tatccacaac tttctttata ataggtatga taatcactct ctaccatatt aattaactct
      121 tttcccccgc aaaattcttc aatgtctaaa ttaataaatt cctctaaaag ggaaagctta
      181 gagtcaatcg aaagcatttt aggtaacata gtccaaaagt cattatcatc gtttttacgt
      241 agtgtataat atgtttgctt ttctagattt aatacataat tatatgcatc ataatctccc
      301 tttttttcaa aaaaattttt catatttaaa aatctactca caaatttttt cttgaaacta
      361 ttcattttct cacctctatt tattcttttt cttgtccata gcataacttt tgaatagagt
      421 aactaaattc aaaagctatt atagtata
//
LOCUS       DAZUKD010000300          432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000300 DAZUKD010000000
VERSION     DAZUKD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.317"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..406
                     /locus_tag="ACG8N9_003019"
     CDS             <1..406
                     /locus_tag="ACG8N9_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4898518.1"
                     /translation="FIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQL
                     LIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYL
                     DTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          174 a           72 c           70 g          116 t
ORIGIN      
        1 ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa aaaatgggga
       61 aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac aaacagaaac
      121 gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa attatcatct
      181 tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg catattcaga
      241 acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa cggagacttt
      301 ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag cggttcatga
      361 actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa attcttgtca
      421 catcctctta tt
//
LOCUS       DAZUKD010000301          421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000301 DAZUKD010000000
VERSION     DAZUKD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.318"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>421
                     /locus_tag="ACG8N9_003020"
     CDS             <1..>421
                     /locus_tag="ACG8N9_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS4898519.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFI"
BASE COUNT          154 a           73 c           87 g          107 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 c
//
LOCUS       DAZUKD010000302          415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000302 DAZUKD010000000
VERSION     DAZUKD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.319"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>415
                     /locus_tag="ACG8N9_003021"
     CDS             <1..>415
                     /locus_tag="ACG8N9_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4898520.1"
                     /translation="VSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKL
                     DDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKG
                     TVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          169 a           62 c           95 g           89 t
ORIGIN      
        1 gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg
       61 cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa
      121 gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag
      181 tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt
      241 taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata
      301 aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg
      361 ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAZUKD010000303          400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000303 DAZUKD010000000
VERSION     DAZUKD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.321"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>400
                     /locus_tag="ACG8N9_003022"
     CDS             4..>400
                     /locus_tag="ACG8N9_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4898521.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDE"
BASE COUNT          144 a           49 c           74 g          133 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac
//
LOCUS       DAZUKD010000304          390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000304 DAZUKD010000000
VERSION     DAZUKD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.322"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8N9_003023"
     CDS             <1..379
                     /locus_tag="ACG8N9_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS4898522.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          135 a           77 c           77 g          101 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac
//
LOCUS       DAZUKD010000305          382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000305 DAZUKD010000000
VERSION     DAZUKD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.323"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..299
                     /locus_tag="ACG8N9_003024"
     CDS             <1..299
                     /locus_tag="ACG8N9_003024"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4898523.1"
                     /translation="KKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRI
                     AIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT          144 a           50 c           71 g          117 t
ORIGIN      
        1 tgaaaaagat gctaaatgtg tctgttactg aatcggcacc tgtaaatatt gaaatcgtac
       61 ctcaaaatgt atctaaaggt gtggtgtttg atcgtttgga atcaatctac aatttaaaga
      121 aacctttgag aatcgcgatc ggagatagtt taaatgactt atctatgttt gaaagtgcag
      181 atattagttt tgcaatggga aatagtcatc aagaaatcat gaaaaaagct gattatgtca
      241 ctgtttcaaa tgataaagac ggagtatctc aagcatttga aagaattctt accatataaa
      301 ctaattaagg tagtatagaa catagaattc taaatacgga aaaatggagc ataaattcca
      361 gtacaaatag tacttcttca gt
//
LOCUS       DAZUKD010000306          382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000306 DAZUKD010000000
VERSION     DAZUKD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.324"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>382
                     /locus_tag="ACG8N9_003025"
     CDS             <1..>382
                     /locus_tag="ACG8N9_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4898524.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIV"
BASE COUNT          140 a           68 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tg
//
LOCUS       DAZUKD010000307          382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000307 DAZUKD010000000
VERSION     DAZUKD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.325"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           99 a           87 c           74 g          122 t
ORIGIN      
        1 tttaatagat aagttaaggg gtgcataaac tgcatccctt aacttatctt attttgctta
       61 aacaatcttg gcccgtaaac tgcataactc atatgtaaat cgctcctttt taggtggcac
      121 aaatgtgagg cattttcact atttccggca attacttcca tgtaaagtat aacccactat
      181 actttgcaag caaagtgtgg gctcgccgag gggtcttgca gacggctctt tcggctcttt
      241 tgagccgagt atcagcgcac ccttatggtg caattagctg atacctgttt gacgatttgc
      301 tgccctacct tttcggtagg tatgcaaacc tcaaaccaaa ataaaaaccc ccttcgactt
      361 cgtcaggggg tttttattta ta
//
LOCUS       DAZUKD010000308          380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000308 DAZUKD010000000
VERSION     DAZUKD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.326"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>380
                     /locus_tag="ACG8N9_003026"
     CDS             <1..>380
                     /locus_tag="ACG8N9_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS4898525.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHK"
BASE COUNT          133 a           71 c           62 g          114 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa
//
LOCUS       DAZUKD010000309          379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000309 DAZUKD010000000
VERSION     DAZUKD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.327"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..>379)
                     /locus_tag="ACG8N9_003027"
     CDS             complement(45..>379)
                     /locus_tag="ACG8N9_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4898526.1"
                     /translation="NYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDW
                     LYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFF
                     IFKIKIFS"
BASE COUNT          143 a           59 c           18 g          159 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattat
//
LOCUS       DAZUKD010000310          364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000310 DAZUKD010000000
VERSION     DAZUKD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.denovo.329"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>364
                     /locus_tag="ACG8N9_003028"
     CDS             <1..>364
                     /locus_tag="ACG8N9_003028"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4898527.1"
                     /translation="IISFTARYDINIKGFDYLCEVAKNLPVGWAIRIAGDGTEEERKE
                     LKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEGLPLVLIEAMTYGLPV
                     ISFDHGGAKEILQDGNYGI"
BASE COUNT          129 a           34 c           80 g          121 t
ORIGIN      
        1 attatttcat ttactgccag atacgatata aatattaaag ggtttgatta tttatgtgaa
       61 gtagctaaaa acttacctgt tggatgggct ataaggattg ctggagatgg tacagaagaa
      121 gaacgtaaag agctgaaaag attaattgag aaaaatggtg tgttagataa aattatttgc
      181 gcaggtgcat tatttggaga tgaattaatc aatcattata tagaaagttc tattttcata
      241 tctacttcta gaagtgaggg actaccatta gtgttgatag aagcaatgac ttatggatta
      301 cctgtaataa gttttgatca tggtggtgct aaggaaatat tacaagatgg aaattatgga
      361 attt
//
LOCUS       DAZUKD010000311        24059 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.036, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000311 DAZUKD010000000
VERSION     DAZUKD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.036"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..2048
                     /locus_tag="ACG8N9_003029"
     CDS             3..2048
                     /locus_tag="ACG8N9_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4898528.1"
                     /translation="MARSIAGVIGATEKHDGYLEGNGYLVSWCIGHLVSFADAGRYDE
                     RFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVCATDAGREGELIF
                     RFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSALCRAKADWLIGI
                     NATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVHITAGGADAVSSR
                     FSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREANRLFGYTAKQTL
                     DYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKGLNLSPQFDRILN
                     SKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGPHIYEAVTATFTC
                     AGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELCEGQTFADVAASV
                     TEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAILEKLVQMGFVQR
                     KGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPDEFMDGIETQARE
                     LVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRACQFVMWKNDRFF
                     EERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGGKYVNYRIEHRS"
     gene            2197..6117
                     /locus_tag="ACG8N9_003030"
     CDS             2197..6117
                     /locus_tag="ACG8N9_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4898529.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLPNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            6118..7062
                     /locus_tag="ACG8N9_003031"
     CDS             6118..7062
                     /locus_tag="ACG8N9_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898530.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            7066..7269
                     /locus_tag="ACG8N9_003032"
     CDS             7066..7269
                     /locus_tag="ACG8N9_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4898531.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(7569..7805)
                     /locus_tag="ACG8N9_003033"
     CDS             complement(7569..7805)
                     /locus_tag="ACG8N9_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898532.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(7988..8359)
                     /locus_tag="ACG8N9_003034"
     CDS             complement(7988..8359)
                     /locus_tag="ACG8N9_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4898533.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(8356..9684)
                     /locus_tag="ACG8N9_003035"
     CDS             complement(8356..9684)
                     /locus_tag="ACG8N9_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4898534.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(9645..9974)
                     /gene="mobC"
                     /locus_tag="ACG8N9_003036"
     CDS             complement(9645..9974)
                     /gene="mobC"
                     /locus_tag="ACG8N9_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4898535.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(10232..10603)
                     /locus_tag="ACG8N9_003037"
     CDS             complement(10232..10603)
                     /locus_tag="ACG8N9_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4898536.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            10748..10933
                     /locus_tag="ACG8N9_003038"
     CDS             10748..10933
                     /locus_tag="ACG8N9_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4898537.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            11091..11258
                     /locus_tag="ACG8N9_003039"
     CDS             11091..11258
                     /locus_tag="ACG8N9_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898538.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            11490..11663
                     /locus_tag="ACG8N9_003040"
     CDS             11490..11663
                     /locus_tag="ACG8N9_003040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898539.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            11876..12543
                     /gene="vanR-B"
                     /locus_tag="ACG8N9_003041"
                     /pseudo
     CDS             11876..12543
                     /gene="vanR-B"
                     /locus_tag="ACG8N9_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
     gene            12546..13889
                     /gene="vanS-B"
                     /locus_tag="ACG8N9_003042"
     CDS             12546..13889
                     /gene="vanS-B"
                     /locus_tag="ACG8N9_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4898540.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            14060..14866
                     /locus_tag="ACG8N9_003043"
     CDS             14060..14866
                     /locus_tag="ACG8N9_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS4898541.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            14884..15711
                     /gene="vanW-B"
                     /locus_tag="ACG8N9_003044"
     CDS             14884..15711
                     /gene="vanW-B"
                     /locus_tag="ACG8N9_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4898542.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            15708..16679
                     /locus_tag="ACG8N9_003045"
     CDS             15708..16679
                     /locus_tag="ACG8N9_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4898543.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            16672..17700
                     /gene="vanB"
                     /locus_tag="ACG8N9_003046"
     CDS             16672..17700
                     /gene="vanB"
                     /locus_tag="ACG8N9_003046"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4898544.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            17706..18314
                     /locus_tag="ACG8N9_003047"
     CDS             17706..18314
                     /locus_tag="ACG8N9_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4898545.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(18566..19570)
                     /locus_tag="ACG8N9_003048"
     CDS             complement(18566..19570)
                     /locus_tag="ACG8N9_003048"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4898546.1"
                     /translation="MQRGGKSRGKAAAAGSGKQKASNGERFLKKIERTGGEAVNRLTR
                     RRKVYEAIHTLRQEKGYEIASLCALSGIPRCSYYKWLHREESASEKENTLLLEEIIRL
                     YSEVKGIYGYRRMTLNVNSRLKRNYNHKRIYRLMKSVHMQAVIRRKKKHYVILEPRVT
                     AENVLNREFTANGPNEKWLTDVTEFKLINGKKAYLSAILDLHDKSIVAYALGQSNNNQ
                     LVFDTFDRAVRANPGAHPLFHSDRGYQYTNRQFKAKLDGIHATQSMSRPGRCIDNGPM
                     EGFWGILKSEMYYLRQFHTFEELKKAIDEYIDFYNTRRLQAKLKGLAPLAYRNQTLVA
                     "
     gene            complement(19453..20136)
                     /locus_tag="ACG8N9_003049"
     CDS             complement(19453..20136)
                     /locus_tag="ACG8N9_003049"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4898547.1"
                     /translation="MSLKGKIPPEVKIQAVEDYLAIRKGSTQIREELGIRLSTFQAWL
                     RKYQTEGPAGLHPKKHVTSYPSSLKLAAVEDYMGGGGSLDAMCRKYGISSHAVLQQWI
                     TLYNQGHREFKTRRAREETDMAEKEKLSQEQKLQAVLYCLEHQLDYRRTSEQYKITYQ
                     QIYSWVKKYQEQGEAGLLDRRGRRRPPSEYSEEEKAAAKLRQLEAENRRLQMENDFLK
                     KLNELEGRR"
     gene            20582..21013
                     /locus_tag="ACG8N9_003050"
     CDS             20582..21013
                     /locus_tag="ACG8N9_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4898548.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            21020..21253
                     /locus_tag="ACG8N9_003051"
     CDS             21020..21253
                     /locus_tag="ACG8N9_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4898549.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            21677..21877
                     /locus_tag="ACG8N9_003052"
     CDS             21677..21877
                     /locus_tag="ACG8N9_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4898550.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            21961..23154
                     /locus_tag="ACG8N9_003053"
     CDS             21961..23154
                     /locus_tag="ACG8N9_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4898551.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(23333..23611)
                     /locus_tag="ACG8N9_003054"
     CDS             complement(23333..23611)
                     /locus_tag="ACG8N9_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898552.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFF"
     gene            complement(23729..23989)
                     /locus_tag="ACG8N9_003055"
     CDS             complement(23729..23989)
                     /locus_tag="ACG8N9_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898553.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
BASE COUNT         6201 a         5902 c         6354 g         5602 t
ORIGIN      
        1 gcgtggcccg cagcattgcg ggcgtgatcg gggccacgga gaaacacgac ggctatttgg
       61 aggggaacgg ctatctggtg agctggtgca tcggacatct ggtgtccttt gccgacgccg
      121 gacgctatga cgagcgtttc aaaaaatggc gctatgagga tttgcccatt ctcccggaaa
      181 catggcagta catcgtcccc gacgacaaga aacagcagtt tgacgttctc tgttcgctga
      241 tgcagcgccc cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc gagggggaat
      301 tgattttccg tttcgtctac cagatggcgg gatgccaaaa gccctttgag cgcctttgga
      361 tttcctctat ggaggacagt gctattaagg acggctttgc ccacctgaaa ccgggcggcg
      421 actatgatcc gctgtatcaa tcggccctgt gccgggccaa ggccgactgg ctgatcggca
      481 ttaacgccac ccggcttttt tccgtcctgt accacaagaa cctgactgtg ggccgcgtcc
      541 agacgccgac cttaaaaatg ctggtggaca gggacgccaa gattaccgat ttcaagaaag
      601 agaaatatca catcgtccat atcacggcgg gcggcgcgga cgcggtaagc agccgctttt
      661 ccgacgctgc cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag gccgtttgcg
      721 tttccgtgac gcgggagaaa aagacggaac agccgcccaa gctctatgac cttaccacct
      781 tgcagcggga ggctaaccgg cttttcggct ataccgccaa gcagaccctt gactatgccc
      841 aggcactgta tctaaagaag ctgctgacct atccgagaac ggatagtcaa tatcttaccg
      901 acgatatgct gcccgccgtg gaaagccttg tgtccggcct gtgggagctg gtgccgtttg
      961 ccaagggcct gaacctttcc ccgcagtttg accgcatcct gaacagcaaa aaggtgtccg
     1021 accaccacgc cattatcccc accgctgaat ttgtgaagca gggctttggc actctggccg
     1081 agagtgaaaa aaagctgctg tccctgatct gctgcaagct cttatgtgcc gtggccgggc
     1141 cgcatatcta tgaggccgtc accgccacat ttacctgtgc cgggcaggag tttaccgcca
     1201 agggcaagac cgtcctttcc cccggctgga aagagattga acgccggttc cgttcttcct
     1261 taaagaccga cgccgacgag gacgccgagg cggtgcggga gctgccggag ctttgcgagg
     1321 gccagacctt tgcagacgtg gccgcctctg tcaccgagca tttcaccacg ccgcccaagc
     1381 cctacaccga agatacgctt ttgtccgcta tggaacgggc cggggccgag gatatgccgg
     1441 aggatgccga gcgcaaggga ttaggcacac cggccacccg cgccgccatt ctggaaaagc
     1501 tggtgcagat gggctttgtc cagagaaaag gcaagcagct cattcccacc aaggacggca
     1561 tcaatctggc tgtggtgctg ccggaggcgc ttacttctcc ccagcttact gccgaatggg
     1621 aaaaccggct gaccgagatc gccaaggggc aggccgatcc tgacgaattt atggacggca
     1681 tcgagacgca ggcgcgggag ctggtgcaga cctattcctg tatcagtgag gaaaagcaga
     1741 aattgtttca ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt gaggctgtct
     1801 atgagggcaa gaaaaactac tactgcggaa accgggcctg ccagttcgtc atgtggaaga
     1861 atgaccgatt ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc gctgcgctgc
     1921 tcaaaaccgg caaagtcaag gtaaagggcc tctattccgt aaagaccggc aaaacctacg
     1981 acgggacggt gcttttggct gataccgggg gcaagtacgt caactaccgc attgaacaca
     2041 ggagctaaag gacagtaaca cagcaagcat agggagtgtg gctacactcc cggtaaaatc
     2101 ccccggccgc gtccctgccg ggccggggat ttttgcttgt tggggaagtc cccaaccccc
     2161 tattttttga aacagaacag agaaaggagt gaccgcgtga acgaagtatt ttctatccaa
     2221 gtccataacc ggcagttttt ggaacagggc aaacaaggcg tctggctgga tttgcccacc
     2281 acaaccgaaa cgctgcaagc cgccctgcgg gatgttggca ttactgccga caatccgcag
     2341 gattttttta tcaacgggta ttcctgcccg gaggacagac accttgccat tccctatgac
     2401 atggtgctgg ccgccgacgt ggacgagctg aatttccttg ccgcccggct gggccagctt
     2461 gacgccgccg agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg cggctttgag
     2521 aatatcggcc agatcatcga ctacaccgaa aatgtggatt actatgtgca tcttcccgac
     2581 gttagggggg atggacagtt gggcgactac tacctgaacc gttccggcat ggtggatatg
     2641 ccggaggaat ggaaagccgg gattgatacg gcgcagtttg gacgccacat tgcccagcag
     2701 gaacaaggcg tttttaccca gtacggctat ctggtgagaa gcggcgacga atggcagcgc
     2761 gtccatgagg gccagccggt gccggaggaa taccgggtga tgcactaccc ccagccggaa
     2821 gctgaccggg aggaaccgca ggcccagcag ccggaggcat ccgaaagccc cgacaaagcg
     2881 ccgcccccgg ttatcccgat catgctggac ggcatgaaca gcaaagaacg catgaaagtg
     2941 attactgacc gtctggaaac cggcgtacag gagctttttg aaagtagccg ctatatggct
     3001 tacttatccg ctatgtccaa gatgcacgat tacagtctga acaatacgct gctgatcgcc
     3061 atgcagggcg gttcgattgt ggcgggctat aacaggtggc aggatgaatt tgaccgccat
     3121 gtgaagcggg gcgagaaagg cattaagata ctggccccgg ccccgttcaa ggttaagcag
     3181 cagatggaga aaatcgaccc ggccacccag cagcccgtga tcggcgcgga cggcaagccc
     3241 gtgaccgaag aacaggaagt gacggttccg gcctttaagg tggtgacagt ctttgacatc
     3301 agccagaccg agggcaagga ttttcccatg ccctatatca gtgaacttac cggcgacgtg
     3361 cagcagtatc aggatttctt tgccgcgctg gaacggacat caccctatcc cataagcatt
     3421 gagccgattt cagacggggc caagggccgc tgcttctacg caaaacagcg cattgccatc
     3481 aacgagggca tgagcgaatt gcagacccta aagaccgcca tccatgagat tgcccatgcc
     3541 aagctccacg ccattgaccg ggaggccccc gccgaggaac aggcggcccg gccagaccgc
     3601 agcacccgcg aggtacaggc cgaaagcgtg gcctataccg tctgccagta ttacgggctg
     3661 gacacgtcgg actattcttt tggctatgtg gccgattgga gcagcggcag ggagttatcc
     3721 gagctgaaag cctcccttga aaccatccgg gccacggcca gcgaattgat aaccgagatc
     3781 gacggccatt ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca ggaggccgtc
     3841 acggttgacg ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga acaggccccg
     3901 gcccttgatc cggcagccga gccgcttgtg actgtgctat ggagcgaaag cccccagcta
     3961 aaggacggcc agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg tctggatgca
     4021 gcatatccgg cagaggcagg ctatgacaaa accgcgttcc gcattgactg tatctttcag
     4081 ggcgagccgg acagctacga ggggcggcag gattttggag atagggacgg ttccctgatc
     4141 gaacatatcc agagctacca ccaattttac gcgcaggacg aaaactggaa aaagcatgta
     4201 atcgctacaa ccggcataga gggctgggag caggacaagg cccatcggga tatgctgtta
     4261 ggcgagtttg tcccctatat gaagctgcat tgcagccttg cccaaatgga acagacggcg
     4321 cagggccgct tacagtccgg ggaaaccctg acgccggagg aaacggccta tttgggggcg
     4381 cttctctctt atgtgcagga gtgccgcccc atgctcaatc agggccagta tcagttgccg
     4441 gagccgccga aactggctga cttcgaccag agcttgcagg actacaaagc acaggtacag
     4501 gccgagatcg cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata cgcggcggct
     4561 ggctatgaag caccccaaca ggacaccttt tccatctacc agcttcgagg cggggaggaa
     4621 acccgcgact atcggtttga gccatacgag cgtttacagg ccgcagggct ggccgtggac
     4681 agtgccaact atgaccttgt ttatacggca ccgctggata aaagcacgtc tttggaggat
     4741 atttaccgca cattcaacat cgaccacccg gcagatttta ggggacacag cctttccgtt
     4801 tccgacgtgg tggtgctgca ccagaatgga caggacaccg cccactatgt agacagcttc
     4861 ggctatatgg aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc cgaactgtct
     4921 acggagcaga acgaaaacat gattgacggc attatcaaca atacgccgcc cgaaccgcag
     4981 ccggaggaac ccgaacagct atcttttgag gatgtggcag ttgcttccgg ccagacacag
     5041 gaggaacccc cggaggctgc gcccccgcaa acggctgtcc gctattatcc catcaatgag
     5101 ggagccgccc gccgcgccaa ggaagccatg agcttttctg actaccggcc cggcagcgcc
     5161 acggcggaat accggcacta tgtcgatcag gccgtggaaa ttgcggagcg ccagaaaaag
     5221 cgtgtggagc cagagtatca cgaaaagata gacagcctcc ttgacaccta cgcccgcaag
     5281 ctggccgaga acatgaataa gggctatgag attaccgccc gcgtcccctc tgtcctgatt
     5341 gccgggccgt ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc cagtgaccgc
     5401 aacatggagg aatttcagta cattcagggc ttgcttgaca aaatccgcag caccggcatg
     5461 ggtggtatca gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa actggaaaag
     5521 ctggaagcgt cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa gcatgggacg
     5581 ctggacggat gcccccatct gaccgaaaga ggcattgaaa acctgaaagc ggatatggcg
     5641 tcgggctggc actatgagaa aaaaccgttc cagtcctggc agctccccaa caataacgcc
     5701 gagatacgcc ggttaaaggg ccgcattgag gaattgaccc gccaaaagga ggcggcctat
     5761 gtgggatggg agtttgacgg cggcactgtg gagatcaacc gggtggccaa ccgcctgcaa
     5821 atcttctttg aggggaagcc ggatgctgct gtacgcgacg agctgaaaag caacggtttc
     5881 cgctggtcgc ccaaggccgg ggcatggcag cggcagttaa acgacaccac aatccgcgtc
     5941 gctgaccgta tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc cttacagctt
     6001 gcggcccgca gggagaaaga aaagccctct atccgggcgc agctcaacgc ggccaagacc
     6061 gaacaggcca agcagccacc ggcgcgggaa aaatcaaagg gattggaggt agagtaaatg
     6121 cagaagtttg aaaatgtgga cgttctggcc gcgctggaac agatcatgcg gcagaacacc
     6181 gcgttttatc aaagtgactt tggcattgac aaagacatta tcaggcggga tgccgccagc
     6241 gatcaggccg cagacaagac cctgctctgg atgtcccggc cctccgggac gtactgtttc
     6301 cgggaacgtg atgttttcct gaaagacacc cgacagtaca acacatggaa attttacggg
     6361 gagcagaccc gtgacaagat actggcctac gcggtggagc tgaccggcac acagggcggc
     6421 actatccggg gcaatctcta tgagctggac tatccgcagc atttccggca cgtcattgaa
     6481 accgcccagc cggtgagtgt gaaccggctc ttttatgagc atggcacccg tgacatcccg
     6541 gagagccagc gttttgacgg aagccctgac cgggtattgg gaaacttttt gtactatgag
     6601 gcccagcccc atgacccggc tgtgctgcag gaggcgctac ggacggagca gcacagccgg
     6661 gggcgggccg tccccggcga tttcaaagtc cacgtcaccg ctttgcatga cagccggatt
     6721 gaaaccgagg cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc taacagcccc
     6781 aataaaacgc atttcatggt ggagatctcc ccgcactttg cggcgattgc ttccaccaag
     6841 gataatgacc ggctctttgc catgctgccc tataaatccc tgtgttttac cgggatgaaa
     6901 ggcaggcatg gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt gagtattcgc
     6961 aggccccggc cctctatccg caagcagcta tccgacagca aacaggccgc cagcccaaaa
     7021 aaggcggcgg ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat gcactttacc
     7081 gtggaggaag aaaacctgat ctgtatgtac cacaatgcag accggcgcag aacgataaac
     7141 aggctcaccg ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc ccagcagacc
     7201 ctttccaagc tggaacgcat gacggatgcc gactatgacg gccagaagtt ccacttcaca
     7261 gacgaataac gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg cgtccggcgt
     7321 cttttctttt tgcctattcc tacgcattta gaacccggta ttgtcgggga taggatgaag
     7381 tattcgcttg cccctgcaaa catgcgggag aagccttgca gcgggcggtt ttcaatccct
     7441 taaatgcgta ggttgcagct ataattagac ccctgaaaca gatatagccg cgcagcgggt
     7501 gtatctgttt caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg gtgcagccct
     7561 cttgccgtct atctctgttc cagcgggaag ctcggcaatg cttccagcag tttcagggaa
     7621 atgcgctgcc gtatatcttc gtcaatgact ttcttctttt tgccgtttgc ctgtctgatc
     7681 tccaccgtgg aaagctcgtt gatgtagtca gcataatggg ccaacacttt ttccgtggcc
     7741 cacctctcac cggcaacggc ggctttgatc gtttcatagt ccagcagctt ttcttgtccg
     7801 tccatcctga tacccccgtc agactgattt tttctatgat accatattcg gaagcatgga
     7861 accagagaaa acggcgcttt tctgctggta aattctacgc atttagaaca gcacttcggc
     7921 ggggatagga taaaacaccc acataccccg ctgaccatgc cggaaaagcc ttgaaatata
     7981 agggctttta cagctctaag tgcgtaggtt tccagtatct tttccgctgg cgctcggcct
     8041 ctttgcggcg gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg ttagacttcg
     8101 gcgtgaacgt ggccccgcac tcacagcagc gtttcacttc gtcccggtct ttggtgatct
     8161 cggcatacag ggccgcatcc agcgggagga cagcggcctt aaaccagcgg cacagaagcg
     8221 aataggaaat gctctgtacg caaatacatt cttcgccgtc gtccagcaac aggcagttgc
     8281 cgtcacaata gttgcagcac tcatgcgtca gcttccgggc cttgcgatat tgagggtaat
     8341 tcattcgtgg aatactcata gctcttgcgt ccattccttt cccggcgtca tacgcaaaat
     8401 gccgtccaca ttctgcttga tgatgccgta ttcctgcatc tgctttttcg cttcgccgta
     8461 cttctcatac agccgttcct tttcctcggc caatcgcttg tactcggctt tcagggcgta
     8521 cagatcgggg agctttttca caccctgtcc ctttagggcc tttgccgcag cttcatacaa
     8581 aatcaacgtg ctttcatgct cacgccggaa ttttgcctta tccgctgcat tgcggtattc
     8641 catagccacg ggccagagct gcttgtaggt agaaatattc tttatcagca ccgccatatc
     8701 cgcaagccgg tgttccatag ctttgagggt agcggcggcc tcgtcgctgg cggcggtgat
     8761 ctcggccact ttgctttcca aatccgggta ggtgtctatc ccatgttcag tcaggaaatt
     8821 caaagtcttt gcggcctgct tcaaattgtg gagcttcgcc cacctctcat agcctgccga
     8881 ctgctgggcc ttaatgttgt tttccagatc aatccgcaaa gaaatcccct tgcgctcttt
     8941 gggcgctctg gtgataacgc ggcctttgat acgctctttg atggcttcct cggtgtaggc
     9001 ttcccccaat gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga aagaaataaa
     9061 ctttcccggc ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat catcaaaatc
     9121 ttttgcctgc gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc agctcgtccc
     9181 tttccgctgg gcgtcccact cggcatagct cttgccctta ctctggccgg gcttcaccac
     9241 ggacaatccg ttttccttgc acagcctgtc gctggtgcgc cggatatagg catagctctg
     9301 tttgttggaa atgtacttgc gctgatttac catatccacg gcacagaaaa taatgtgatt
     9361 gtggacgtgg cccttatcaa tatgggtagt cagcacatag ggatatttcc cttgcagcac
     9421 ttcctcggca agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt cgccgggggc
     9481 aaaggattgt atcaggtggt ggcccagatt attgcccttt tggtatgcct gcgccagcag
     9541 catttcaaat tcaatgtccg ctgtttcata agagcaggca aaggaggcca ccagcagctt
     9601 gccgtcagtt ttgtccgggt tctcgatgta gtcaagggct tttttcagcg ttgacttgat
     9661 aggatgtatc tttgtaactg ccatatctgc gccagcgccc cctttatatc ctcgatgtcc
     9721 tgtgcgtaca ccggccccat accgttcagc ctctttgcaa tctgattgac gttgacgccg
     9781 atcttctgta tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt gacgatatag
     9841 ccgtcaatga gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag cagcgccatt
     9901 ttctgttcaa tcatagcccg ttcctccggc gtcacaggga cgcgcagcac gatttttcgc
     9961 tttctgtttg ccatgcttca ccgtcctttc cattcagggg tttggggatt tccccaacaa
    10021 gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact
    10081 ccctatgctt gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta
    10141 cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag
    10201 ctcccgctaa aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg
    10261 gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg
    10321 ggcagagttt cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct
    10381 ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg
    10441 gcagactggg aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta
    10501 ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg
    10561 cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta
    10621 cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta
    10681 tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag
    10741 gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg
    10801 atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat
    10861 ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc
    10921 ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa
    10981 gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga
    11041 taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc
    11101 gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg
    11161 tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct
    11221 ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat
    11281 accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac
    11341 atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt
    11401 ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac
    11461 cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca
    11521 aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg
    11581 ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg
    11641 ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag
    11701 gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc
    11761 atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga
    11821 aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc
    11881 gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct
    11941 ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt
    12001 ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca
    12061 tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct
    12121 gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa
    12181 gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc
    12241 gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact
    12301 ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt
    12361 gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga
    12421 ctttgagggt gatggcagca cagtccacac tcccacactc atatcaaaaa tttgcgggcc
    12481 aagctgccgg aaaatatcat caaaaccatt cgcggtgtag gataccgatt ggaggaatca
    12541 taataatgga aagaaaaggg attttcatta aggttttttc ctatacgatc attgtcctgc
    12601 tactgcttgt cggtgtaaca gcaacactgt ttgcacagca atttgtgtct tatttcagag
    12661 tgatggaatt acagcaaaca gtaaaatcct atcagccatt ggtggaactg attcagaata
    12721 gcgataggct tgatattcaa gaggtggcag ggctgtttca ctacaataac caatcctttg
    12781 agttttatat tgaagataaa gagggaagcg tactctatgc cacaccaaat gccaatacat
    12841 caaatagttt tagacccgac tttctttatg tggtacatag agatgataat atttcgattg
    12901 ttgctcaaag caaggcaggt gtgggattgc tttatcaagg gctgacaatt cggggaattg
    12961 ttatgattgc gataatggtt gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa
    13021 tgacaacgcc gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg
    13081 taccgccgcc gctggagcga aaggatgagc ttggcgcact ggctcatgat atgcattcca
    13141 tgtatgtcag gctgaaagaa accatcgcaa ggctggagga tgaaatcgca agggaacatg
    13201 agttggagga aacacagcga tatttctttg cggcagcttc tcatgagcta aaaacgccca
    13261 tcgcggctac aagcgttctg ttggagggaa tgcttgaaaa tatcggtgac tacaaagatc
    13321 attctaagta tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa atcatttccg
    13381 aaatactgga gcttgtcagc ctgaatgatg ggagaatcgt acccatagct gaaccgttgg
    13441 acatagggcg cacggttgcc gagttgctgc ccgattttca aaccttggca gaggcaaaca
    13501 accagcggtt cgtcacagat attccagccg ggcaaattgt cctgtccgat ccgaggctgc
    13561 tccaaaaggc actatccaat gtcatattga atgcagttca gaacacgccg cagggaggcg
    13621 aggtacggat atggagtgag cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg
    13681 gcgttcacat tgatgatact gcgcttccaa ggctgttcac cccattctat cgcattgatc
    13741 aggcgcgaag cagaaaaagt gggcgaagcg gtttaggact tgccatcgta caaaaaacgc
    13801 tggatgccat gagcctccag tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc
    13861 tggatttacc gctcacatca acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca
    13921 ggtataaccc tgccggtctt tttttgctgc tacaggaaaa ctacagactg gctacaggga
    13981 aagtacagat gcgcttgcca taataacaat cgtaccagcc acaaatcgta gttttattgc
    14041 aaaggaggca ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg atcatcacaa
    14101 acggcatatg aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt
    14161 cacagtctgc acgctgtttt tggggtggag actggcttcc gtattggagg caacacagat
    14221 accgcccatc cctgcaactc atacaggcag cagcactgac gtagtggaga atttggagga
    14281 aaacgctctt gccaccgcca aagaacaggg agatgaacag gaatggagcc tgattttagt
    14341 gaacaggcag aaccccatcc ccgcacagta cgatgtggag cttgagcaac tatcaaatgg
    14401 tgagcggata gatattcgga tttctcccta tcttcaagat ttgtttgatg ccgcaagaac
    14461 tgatggagtt tacccgattg tcgcatccgg ataccgaaca acagaaaaac agcaagaaat
    14521 tatggatgaa aaaattgccg aatataaggc gaaaggctac acctctgcac aggctaaagc
    14581 ggaagcagaa acttgggtgg ccgtgccggg aacgagcgag catcagcttg gtcttgctgt
    14641 ggatatcaat gcggacggaa ttcattcaac aggcaacgag gtttatagat ggctggatga
    14701 aaacagctat cgttttggtt ttattcgtcg ctacccacca gacaagacag agataaccgg
    14761 tgtgagcaac gagccgtggc attaccgata tgttggcatc gaagctgcca cagagatgta
    14821 caaccaaggg gtttgccttg aggaatattt aaaaccagaa aaatgagaaa aggatgttat
    14881 gttatggaca gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat gagacgggcg
    14941 cagagaaaaa tgtgctttta tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg
    15001 ataggggaaa aatcgctttc ccatttgctc tttgaaacgg attgcgcatt atacaaccac
    15061 aataccggat ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa gctggcagca
    15121 aagaccttaa acggcttatt gataagaccg ggggaaacct tttctttctg gtggctggta
    15181 cgccatgcgg acaaagatac cccctataaa gacggcctta cggtgaccaa tggtaaactc
    15241 accaccatgt cgggcggcgg tatgtgccag atgagcaatt tactattttg gatgttcctg
    15301 catacgccat tgacaattat ccagcgcaga ggtcacgaag taaaggagtt tccagagcca
    15361 aacagtgacg agattaaagg ggtggatgca accatttcag agggctggat tgatttaaaa
    15421 gtgcgaaacg ataccgactg cacctaccaa atatgggtga ccttagatga tgagaaaatc
    15481 atcggtcagg tgtccgccga caaagagccc caagcattat acaaaattac aaatggcagt
    15541 atccagtatg tccgtgaaag tggcgggatt tatgaatatg cccaggttaa acggatgcaa
    15601 gttgccttag gtaccgggga aataatagat tgcaagctgc tttatacaaa caaatgcaaa
    15661 atctgttatc ccctcccgga aagtgtggat attcaggagg agaaccaatg agaaaaagtg
    15721 tgggcattac tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc
    15781 cggattttca tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat
    15841 tggccgctgg caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa
    15901 ttcttgcgct gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc
    15961 acattgatac gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc
    16021 cggacagcgt tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac
    16081 ccaccatgca cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac
    16141 tgggggatat gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa
    16201 ggctgcgggg atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag
    16261 attatgtcca gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc
    16321 tttgtgcgga tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg
    16381 catttttaat caacaccggg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt
    16441 tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg
    16501 tttataccga ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga
    16561 tgccgaatgt gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata
    16621 ctacagaaaa aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga
    16681 ataaaagtcg caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc
    16741 gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt
    16801 acaaaaaacg gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt
    16861 ctccccgcca tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc
    16921 gaatacgaaa cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag
    16981 gatggtgcga tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt
    17041 caaagctccg cagcttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc
    17101 atcgccgttc ccgaatttca aatgattgat aaaggtgaca agccggaggc gggtgcgctt
    17161 acctaccctg tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta
    17221 aacggtacgg aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc
    17281 ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat
    17341 gatttgattg tcggcgaagt ggatcaaatc cggctgagcc acggtatctt ccgcatccat
    17401 caggaaaacg agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt
    17461 ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga
    17521 tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat
    17581 gaggtcaata ccctgcccgg ttttacatcg tacagccgct acccacgtat gatggccgcc
    17641 gcaggaatca cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga
    17701 taagtatgga aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg
    17761 ccaagtacgc cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc
    17821 gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg
    17881 cgcttggcta tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct
    17941 tcctgcgttg ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt taccccaata
    18001 tagagcgagc cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg
    18061 gaagcgcaat tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa
    18121 gtaacttcga ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag
    18181 aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc
    18241 gctttgaatg gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt
    18301 ttgccgtttc ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat
    18361 gctggtcatg gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca
    18421 aattatagta ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct
    18481 ttcccacgcc cattttgtat tttgctggcg gcggttttga gaaatgaacc gccccctgtc
    18541 aagtagacag atgaaaaaag aaaaattacg ccacgagggt ctggttcctg taagcaaggg
    18601 gagccaggcc ctttaactta gcttgaagcc gccttgtatt gtaaaagtct atgtattcgt
    18661 cgatggcttt cttcaattcc tcgaaggtat gaaattgccg gaggtagtac atttcagatt
    18721 tgagaatccc ccagaagccc tccatagggc cgttgtcgat gcagcggccg ggccgggaca
    18781 tactctgcgt tgcatggata ccgtccagct ttgctttgaa ctgccggttg gtgtactgat
    18841 agcccctgtc gctgtgaaac agaggatggg cgccggggtt ggctctcacc gctctgtcaa
    18901 aggtgtcaaa aacaagctga ttgttgttgg attggcccag tgcatacgca acgatgcttt
    18961 tgtcatggag atcaaggatc gcactcaggt atgctttctt cccattgata agtttgaatt
    19021 ctgtgacatc tgtcagccat ttctcattgg gaccatttgc cgtaaattct ctgttcaata
    19081 cgttttccgc ggtaacacgc ggttctaata tgacatagtg ctttttcttt cggcgtatca
    19141 cggcctgcat atgtacactt ttcatcaggc ggtaaatccg cttgtggttg tagtttcttt
    19201 tgagccggct gttcacattc agcgtcatac ggcgataacc gtagatgcct tttacttcgc
    19261 tgtacaaacg gatgatttct tccagcaaga gggtattttc tttttctgag gcactttctt
    19321 cccggtgaag ccatttgtag taagaacagc ggggaatccc ggataacgcg cataacgatg
    19381 cgatttcata tcctttttcc tgacgaagcg tatggatcgc ctcatagacc ttcctgcggc
    19441 gtgtgagcct gtttaccgcc tcccctccag ttcgttcaat ttttttaaga aatcgttctc
    19501 catttgaagc cttctgtttt ccgcttccag ctgccgcagc tttgccgcgg ctttttcctc
    19561 ctcgctgtat tcggaaggag ggcggcgtct cccccggcgg tcaagcaacc cagcttctcc
    19621 ctgctcctga tattttttga cccagctata gatctgctga taggtgatct tgtactgttc
    19681 gctggtccgc cggtagtcca gttgatgttc taagcagtat agtactgcct gcagcttttg
    19741 ctcctgcgat aatttttctt tctcggccat atctgtttcc tcccgcgctc tgcgtgtctt
    19801 gaactctcta tggccttgat tatacaacgt aatccattgt tgtaaaaccg catgggagga
    19861 aatcccgtat ttgcggcaca tggcatccag cgagcctcct ccgcccatgt agtcttctac
    19921 tgccgccagc ttcagtgagg atggataact tgtaacatgc tttttcggat gcagacccgc
    19981 aggcccctcc gtctgatatt tccgcagcca tgcctggaaa gtgctcaatc gtatgcctaa
    20041 ctcttcccgg atctgtgtgg aacccttacg gatcgctagg tagtcctcca cagcctgaat
    20101 ctttacctcc ggcggtattt tgccttttaa actcatgata attgctccct ccgtttcgac
    20161 agtttaattt ttttgctgtc tacttcagag ggagcatatc aaaatcaagg ccgccgttct
    20221 tttctggcag caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt
    20281 gccatgctct aatccacttc caccactcaa aaaaagcctt gcccctccac agggctattc
    20341 cggttatgag tatgtactat accatgtaaa tcagcagcat agcaaagcat tttctcgccc
    20401 ggtaggtttg cgacctgccg ggcgattttt gtcgtctttt gcggcccggc tgtccccaaa
    20461 aaaatttttt gagaaatttt caaaaaggag gtatttagcg ggtagctggc ggccttgtca
    20521 aagtcttgtt agcggaaagg gagagaacca cctaagtccc ccacagaaag ggggtgagaa
    20581 gatggatgct atcccccgcg actatgaagc gcggtgcatg ttcgatgctt tttgcaagac
    20641 ggtactgcgt aacgaggcca aaagctactt ggctgaaatg aagcgccgcc gtgaccgtga
    20701 agtgtcacta agctctttgt cacaggcaga tttggacaag ctctgtacag tagatcacta
    20761 ccccagcgac agctttacgt tttcgtcgca tggctatgac ctacatatca acaatgagct
    20821 tgttgccgaa gcctttgccg cgctgcccag catggagcag agtattttaa tcctgcactg
    20881 tgttctggat atggcagatg gtgagatcgg cggccttgtg ggaatgtccc ggagtgccgt
    20941 tcagcggcac aggacaaata ccttatcgga gctgcggaaa cagctaaagg cgttgatgcc
    21001 gaagggaggt tagacgcgga tgaagaaccc gaactacaag ggcattgagc ttttgcccct
    21061 ttcggtgatc gaggccgccc gcgctgggga tgccggagcc gtagagcgcg ttttgcagta
    21121 ttacagcggt tacatgaaca agctgtgtac ccggacgctc tatgacgaaa acggctatcc
    21181 gcatgtgtgt ctggacgagg ccatgaagcg ccgtctggaa atcaagctca tccacgctat
    21241 cgtcacgaac tgacgaaccg gccccggcag catgaaacgg cttacccttt ccccgtttcg
    21301 gtgttttccc ggtggcctga tcgcaccttg ataaatatgc ccgcaagata acggcggcgc
    21361 atcatagggc atacggacac attctgtctg tgccgagccg gacggctggt gcgccatgat
    21421 acttttccct acgggaaaag cggagcgagg aacaccacgc cgcaatgtga gcgtggcggc
    21481 ttgcgggcga cgacgtacag gcaggactat gatactccct tatagccaca gtccgagcgt
    21541 taaaagcgtc gcaggcaatg ggtagggccg cgtgagaacc acgcgggggt gaaagtcccg
    21601 tggagctgtg ccagcagccg tccgtctatg cccctaaatc acacaaacct ttgaatttgt
    21661 gaaagggggc tgcattatga accatgatgt gcctatttgg gaaaaataca cgctgacgat
    21721 tgaggaagcg tctaaatatt tccgtatcgg agaaaacaaa ttgcgtaagc tggccgagga
    21781 aaatccggcc tctggctggg tcattatgaa tggcaaccgc atccagatca aacgcaagca
    21841 gtttgagaaa ataattgaca ctttggacac aatctaatag tgaaaaaggg ccgcagatat
    21901 gttataataa tttataagca tatatgcggc tctttccgtc atggaaagga gcatgacgag
    21961 atgtcagaaa aaagacgaga cagcaaaaat cgcgttttgc ggtctggaga gagccagaga
    22021 aaagacggac gatatgctta caaatatgtt gatacctttg gaaaaccgca gtttgtttac
    22081 tcatggaagt tagtgcctac ggataagacc ccggcaggta aacgggagga tatttcactc
    22141 cgggaaaagg agaaagaaat ccagaaagac cttgacgacg ggatagacac catcggcaag
    22201 aaaatgaccg tctgccagct ctacgccaag cagatcaggc accggggcaa tgtgaagcac
    22261 aacaccaaac tgggccggga acggctgatg cgtattctgg aacaagatcg gctggggagc
    22321 tgcccgattg acagtgtgaa gctgtccgac gcaaaggaat gggcgcttcg gatgaaagaa
    22381 aagggccttt cttataagac catcaataac gacaagcgtt ccctgaaagc ggctttttac
    22441 accgccatac aggacgattg catccgaaaa aacccgtttg acttccagct tagtgacgtt
    22501 ctggacgacg atacggaacc aaaggtgcct cttacaccag cacaggaaga aagttttctg
    22561 tcctttattc aaggtgataa ggtttaccaa aagcattatg acgcaatcgt tattctgctg
    22621 gggacagggc ttcgcatttc ggaactgtgc ggactgacgg acaaagattt ggattttgaa
    22681 aaccgggtaa tcatcgtgag ccaccagctt ttgagaaata ccggcgtcgg ttactatatt
    22741 gacgaaccca agacccaaag cggcgtcaga aaaatcccca tgaatgagga agtgtatcag
    22801 gcgttccagc gagtgataaa aaaccgcaaa ggtgcaaagc cgttcattat tgacgggtat
    22861 gccaatttcc tgtttctcaa acagaacggc tatcccatga cggcggtaga ttatggcggg
    22921 atgtttggca ggcttgtgaa gaagtacaac aagagccatg aagaagcgtt gccgaaaacg
    22981 acgacgcccc atgctatgcg tcatacattc tgcacccggt tagccaatgc gggaatgaac
    23041 cccaaagcct tgcagtacat tatgggacat tccaatatca ccatgacgct gaactattat
    23101 gcacacgcca cattcgattc cgcaagggca gaaatggaac ggctggcagc ctgacacaag
    23161 gccggtttta ctactccatt tactactttt gagggggaaa tggtgagagg taataagaac
    23221 atttgtaagt atctaacaat atcaaaaatg ccggaaaagc ccggaaacac gggctatacc
    23281 gacattcaag aacttctaaa aagataatct aaaatcccat ataattttcc tcctaaaaaa
    23341 atagcacaat ggcaaagatc gtaagagaga tcataagccc caatgaatga ctaaaaagaa
    23401 aagcagaatc acatgccata tcatcccata tttcttcaag aaaataaata agcgggaaaa
    23461 agggaaggaa tagtagaaca tcgatccagc accatttttt tcttcttttg aaataacata
    23521 aaacaataaa catgaacgtg cagggaagta aaaacaaagc tttactcata actaacaaaa
    23581 taccagctcc ttttattttt ttctccccca cattcaaaga gtaacaatgg tatgtactta
    23641 ataatagaaa aatggatcaa cttgtatgaa taacagctga aaagatctgc accaaataaa
    23701 aaacttccag ccaaaactga ctggaagttt atttttttct gaaatattct aacacaaata
    23761 tgaaaaagat ggccgaagct aataagccga tttcgtagca aaacaatgag ataccagagt
    23821 cgtaataata aaatcctcta atcttatgga taaaatagat catcggacaa gcagataaga
    23881 gtaagtattt gttaggcctt ttgaataagt aaaagaaagc actggtcatt atgataaatg
    23941 gtatccaaaa caaaagttta cttaagacaa tcaaatgatc aactcccata ttttttacca
    24001 agagagatca taacatcttt atcgtaaaaa caaaatattt ttttgctcat tttgaaaat
//
LOCUS       DAZUKD010000312        12200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.080, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000312 DAZUKD010000000
VERSION     DAZUKD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.080"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..238
                     /locus_tag="ACG8N9_003056"
     CDS             14..238
                     /locus_tag="ACG8N9_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898554.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            355..852
                     /locus_tag="ACG8N9_003057"
     CDS             355..852
                     /locus_tag="ACG8N9_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4898555.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            939..1331
                     /locus_tag="ACG8N9_003058"
     CDS             939..1331
                     /locus_tag="ACG8N9_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4898556.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1315..3762
                     /locus_tag="ACG8N9_003059"
     CDS             1315..3762
                     /locus_tag="ACG8N9_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4898557.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3765..5948
                     /locus_tag="ACG8N9_003060"
     CDS             3765..5948
                     /locus_tag="ACG8N9_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4898558.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5945..6946
                     /locus_tag="ACG8N9_003061"
     CDS             5945..6946
                     /locus_tag="ACG8N9_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4898559.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6961..7875
                     /locus_tag="ACG8N9_003062"
     CDS             6961..7875
                     /locus_tag="ACG8N9_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4898560.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8189..8236
                     /locus_tag="ACG8N9_003063"
                     /pseudo
     CDS             8189..8236
                     /locus_tag="ACG8N9_003063"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8252..10171
                     /gene="tet(M)"
                     /locus_tag="ACG8N9_003064"
     CDS             8252..10171
                     /gene="tet(M)"
                     /locus_tag="ACG8N9_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS4898561.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10269..11642
                     /gene="tet(L)"
                     /locus_tag="ACG8N9_003065"
     CDS             10269..11642
                     /gene="tet(L)"
                     /locus_tag="ACG8N9_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS4898562.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         3819 a         2182 c         2562 g         3637 t
ORIGIN      
        1 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
       61 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
      121 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
      181 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
      241 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
      301 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
      361 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
      421 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
      481 tatgaggaat acgccattca tgattacgag ttacccttta cggttgacga atacacttct
      541 attagcgaac tcaatcgcct atgggaaatg gtatcggagt tgccagaaga actacaatcg
      601 gagctatctg ctctgctcac tcatttttca agtattgaag aactaagcga acatcaagaa
      661 gatattatca ttcattccga ttgtgatgat atgtctgacg tggcacgcta ctacattgaa
      721 gaaacgggtg ctttaggcga agtgccagcc agtcttcaaa actatattga ttatgaatcc
      781 tatggtcggg atttagacct ttcgggaacg tttatctcaa ccaatcacgg gatttttgaa
      841 atcgtctatt aaatctgtcg gtacattctt actggcagat tttcttgttt acggggtagt
      901 caaaagacta tccccatttt tatgaaagga ttgattacat gaagaaaata cgaagctata
      961 ccagtatctg gtctgttgag aaagtgttgt actccatcaa tgactttaga cttccgtttc
     1021 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtt atgatacttg
     1081 gcaacttgcc ccctctttct atgatagagg gagcatttct caaatacttt gggattcctg
     1141 tggctttcac atggtttatg tctacaaaaa cctttgatgg taaaaagcct tatggatttt
     1201 tgaagtctgt cattgcttat gcactgcgac gaaagctgac ctatgcagga aaaaaagtaa
     1261 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     1321 tatccaatta aatatattga aaacaatctg gtctggaata aagatgggga atgttacgct
     1381 tactatgagc ttgttcccta caattactca tttctaagtc cggaacagaa aatacaagta
     1441 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaagattca tgctttacaa
     1501 atcagtacag aatccagcat actttctgca caagaacgtt ccaaaaatga agtcactgga
     1561 aagctcaaaa cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     1621 attggcgaaa atcaagtgga ctaccgtttc tttatcggtt ttaagttgct tctcaacgat
     1681 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgcttttac tgattttgtc
     1741 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     1801 cgttttcaga agatggaaaa gcttttagaa aataaaatct cccgtcgttt caaaatccga
     1861 agattagata aggatgactt cggctatctg attgaacacc tttacggaca gacaggcact
     1921 gcctatgaag agtatgagta tcctctatca aagaaaaagc tggaacatga aacactgatt
     1981 aaaacctatg acctcattaa gcctactcgc tgtctggtgg aagaaaaaca gcgatatttg
     2041 aaaatccagc aggaagacga aaccgcctat gtagcttact ttaccatcaa cagtattgtc
     2101 ggcgaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttaccttt
     2161 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     2221 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     2281 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     2341 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     2401 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     2461 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     2521 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     2581 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     2641 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     2701 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     2761 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagca
     2821 gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcaat
     2881 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtaattat gaaaaatacc
     2941 aaagattctg aatcactggc tattgatatt ttgacattcc ttacggggat ttcctctcgt
     3001 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagtg
     3061 cgagggttga tgaaagtgat tgaagaatta cgggttgaga atacgccact aagtaccagc
     3121 atagccgacc atatcgaaag ttttacagac tatgactttg cccatctgct ttttagtgat
     3181 ggttatgtag agcagtctat cagccttgaa aaacaactga acattataca ggttgccgac
     3241 ttggtacttc ctgacaagga aacttccttt gaggaatata ccacaatgga gttactatcg
     3301 gtagcaatgc tgattgtcat tagtaccttt gcgttggact ttatccatac agaccgaagt
     3361 attttcaaga ttgtagactt agacgaagca tggagcttct tacaggtggc acaagggaaa
     3421 acactatcta tgaagctggt tcgtgctggt cgtgctatga acgctggggt atatttcgtg
     3481 acccaaaata cagatgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
     3541 gcctttcgtt ccactgacct taacgagatt aaaaaaaccc ttgccttttt tggtgtagac
     3601 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
     3661 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
     3721 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatat
     3781 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
     3841 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
     3901 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
     3961 actatcaact ggatttttat gtggataata gctggggctg gctaccgtgg aactggtcgg
     4021 acggaattgg aaaacaagtc atgtatggac tatatgccat taccaatttt atttggacaa
     4081 tcagtcttta tgtttccaat gcgacgggtt acttagtaca ggaagcctat tccttagact
     4141 tcatttccgc tacagcagat tccattggca agaatatgca gacccttgca ggggttacgc
     4201 ctagtggact atcaacagag ggtttctatg ttggattcct tttactcttg attttggttc
     4261 ttggggttta tgttgcctat acaggactga taaagagaga aaccacaaag gcaattcatg
     4321 ccctcatgaa ttttgtgctg gtttttatcc tatcggcttc ctttattgcc tacgctcccg
     4381 actacattaa aaaaatcaat gacttttcat cagacatcag caacgctagt ttgtcactgg
     4441 gtacaaaaat tgtcatgccc cattcagata gtcaaggcaa ggacagtgtg gacttgatac
     4501 gtgacagcct attttccata caggttcagc aaccgtggct actgcttcaa tataacagtt
     4561 cagacattga aagtattggt attgaccgtg tggaaagcct gctctcaacc agtccagatt
     4621 ccaacaatgg ggaagacaga gaaaaaattg ttgcggaaga aattgaagat agaagcaata
     4681 ccaatatgac gattacaaag acgattaacc gtttaggtac agtcttcttc ctatttgtct
     4741 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
     4801 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
     4861 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
     4921 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
     4981 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
     5041 actttaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
     5101 tgggaagtcg tatgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
     5161 agcggaaact tggacgctcc gtgactgctt taggggctgg gtctgccatt gccagtgcta
     5221 cgggtaaaaa aggacagtcg ggttcgggaa gttctgcgag tacacaagca gatcactctc
     5281 gaccaaacgg gcaggaaaaa tcaacactag gaaagcgtat cggtcaaacc atcggtacag
     5341 tagctgatac caaagacaga atggtagata ctgctggtaa tttgaaagaa caggttaagg
     5401 atttgccgac caatgcaaga tatgcagtat atcaaggaaa atctaaagtg acggataatg
     5461 tccatgattt aaccagaagt atttctcaaa ccaaagcgga taaagccagt ggacgcaaga
     5521 aacagcagga acaaagacgg aaaaccattg cggagcgtcg ctctgaaatg gaacaggtca
     5581 aacagaaaaa acagccagct tcttccgttc atgaaagacc agccactaag caagaacaat
     5641 ctcatgatgg acagacttca aggcaaacaa ctgtacaggc ttcctatagg gaatctcaac
     5701 aagccaaaca agagcgtcca acagttaagt ccgattcttc aagtttaaaa acggaacgtc
     5761 aaagtaatac agttcaagaa agaacggttc aaaagccagt aacttcaact gcaccaacag
     5821 atagagcttc acaacgttca atcacaaaag aacgtcagtc tactgttcaa agagtaccac
     5881 tacaaaatac aaaaagtaga ccaccaatca aaaccgccac cattaagaaa ggcagtaaga
     5941 aaccatgaag ctgaaaactt tagtgattgg tggttcggga ttattcttga tggtcttctc
     6001 actgcttctg tttgtcgcca ttttattttc agatgaacaa gatggcggtt tttccaacat
     6061 tcactatggc ggtgtggatg tttccgcaga agtgctggct cataagccta tggtagaaaa
     6121 agttgccaaa gaatatggaa ttgaagaata cgtcaatata cttcttgcga ttatacaggt
     6181 ggaatcgggt ggtacagcag aagatgttat gcagtcctcg gaatccctcg gtcttccacc
     6241 taattcattg agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgagttatt
     6301 agccagtagc gaaaggcttg gtgtagattt agaatcggtt atccagtcct acaattatgg
     6361 tggtggtttc ttaggatatg tggctaatcg tgggaataaa tatacctttg aactggctca
     6421 aagtttctca aaagagtatt cgggtgacga aaaagtgtct taccccaatc ccatagccat
     6481 acctatcaat gggggctggc gatacaatta cgggaatatg ttttatgtgc aactggtaac
     6541 gcagtatctt gtcacaacag agtttgatga tgatacagta caagccatca tggacgaagc
     6601 tctgaaattt gaaggctgga agtatgtcta tggcggagct tccccgacta cctcttttga
     6661 ttgtagcgga ctgacacaat ggacgtatgg aaaagctgga atcaacttac cacgaacagc
     6721 acaacagcaa tatgatgtaa cccggcatat cccattattg gaagcaaaag ccggcgattt
     6781 ggttttcttt cattctacct ataacgctgg ctcttatatt actcatatcg ggatttattt
     6841 aggcgataac cgtatgtttc atgcaggcga ccctatcggt tatgccgact taacaagctc
     6901 ttactggcaa cagcatttag tgggagcagg acgaatcaaa caataagaaa ggacgattca
     6961 atgatgaaat tcggaaaaaa tcagaataca gaaaaacaga caccaaagga aaagaaacct
     7021 cgtgtctata aggtcaatcc tcgtaaaaag gttgtgattg tcctatgggt acttttagga
     7081 cttagtttca gctttgcgat attcaagcac tttacagcca ttgataccca taccattcat
     7141 gaaaccacca tcatagaaaa ggaatatgtc gatactcatc atgtggaaaa ttttgtagag
     7201 aactttgcga aagtctacta ttcatgggaa ctaaacgaca agtccattga taatcgcata
     7261 gaaaacctga aagcatatct gacagaggaa cttcaagctc tgaatgtcga tacagttcgt
     7321 aaagatattc ctgtatcgtc ttctgtaaaa ggatttcaga tatggacggt agaagcagat
     7381 ggcgacaatc aatttgatgt aacctacagt gtagaccaac tcattacaga gggggaaaat
     7441 acaaagaccg tccactctgc ttatatagtg agtgtctatg tagacagtac tggaaatatg
     7501 gtcctcatta agaatccgac cattactagc ataccaaaga aatcagacta taaaccaaaa
     7561 gctattgaaa gtgagggtac ggttgattcc attacaacca atgaaatcaa tgagttttta
     7621 acgacgttct tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat
     7681 gacgggatat taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt
     7741 tacaatcgta aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag
     7801 accaaagcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg
     7861 aagattatag aataacaaat attggtacat tattacagct attttgtaat cacgtactct
     7921 ctttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta
     7981 tctatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
     8041 atagtgggaa caaataattg gatgtccttt tttaggaggg cttagttttt tgcacccagt
     8101 ttaagaatac ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat
     8161 gcctatggtt atgcataaaa atcccagtga taagagtatt tatcactggg atttttatgc
     8221 ccttttgggc ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
     8281 tcatgttgat gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
     8341 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
     8401 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
     8461 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
     8521 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
     8581 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
     8641 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
     8701 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
     8761 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
     8821 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
     8881 ttgctccttg taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct
     8941 tatagaagtg ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg
     9001 cggaaatgtc ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct
     9061 ttatggcgga atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa
     9121 aattacagaa atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc
     9181 cggggaaatt gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac
     9241 aaagctattg ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt
     9301 tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga
     9361 cagtgacccg cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt
     9421 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
     9481 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
     9541 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
     9601 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
     9661 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
     9721 tggttggaat gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt
     9781 tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa
     9841 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
     9901 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
     9961 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
    10021 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
    10081 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
    10141 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
    10201 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
    10261 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
    10321 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
    10381 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
    10441 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
    10501 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
    10561 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
    10621 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
    10681 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
    10741 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
    10801 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
    10861 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
    10921 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
    10981 atatcaagaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
    11041 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
    11101 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
    11161 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
    11221 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
    11281 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
    11341 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
    11401 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
    11461 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
    11521 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
    11581 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
    11641 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
    11701 aattcaaatc tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc
    11761 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca
    11821 ataaaaattt tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata
    11881 aaaattggca agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc
    11941 tgtcccttgc tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag
    12001 agggcaggtt tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat
    12061 ggttttggtc ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata
    12121 atgtgttata ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg
    12181 ccacctaaaa aggagcgatt
//
LOCUS       DAZUKD010000313         8244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.125, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000313 DAZUKD010000000
VERSION     DAZUKD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.125"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1764
                     /locus_tag="ACG8N9_003066"
     CDS             <1..1764
                     /locus_tag="ACG8N9_003066"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4898563.1"
                     /translation="LFSIASISAGYHVIILEGIGETIENSKRQHKFTPNSHILMGIAA
                     IGASLIGNFWEGTLLVLIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLIMQDGNTT
                     IVNVSELKVGDQLQVLNGDQVPIDGVIVSGSTSIDESSINGESIPKEKSKGDGVFGST
                     INGTGTFTMEVTKETNDTVFSKILQLVNQNQENQTKASSIIQKFEPKYVKFVLIAIPL
                     FILLAPMLFDLTWSQSIYRGLVLLVAASPCALAAATVSVTLSATSNLAKRGVLSKGSA
                     YLSQLADIQAIAFDKTGTLTKGKPEVTNYYFSDSVNEENMIDIVVALEKESNHPLASA
                     ILDKFEPKNKLAIEVENQIGKGLIGDYNENTYRIGKPTSFEAVSDDYTRLNKEWASEG
                     NTVVYVSENEKVIGLIALMDIPSEHAKATIDYFKKCGIHTTLITGDSEMTGQAVGKQL
                     GVDQVIANVMPEDKSNIINEQKETYGITAMVGDGINDAPALVKADVGIAMGEGTDVAV
                     DVSDLVLMKNDLSKLVKAHEISLKMNRVIWQNIIFSMVVVAFLITVSLVGLTDIAISV
                     IVHEGSTLVVILNGLRLLKSK"
     gene            1858..2079
                     /gene="relB"
                     /locus_tag="ACG8N9_003067"
     CDS             1858..2079
                     /gene="relB"
                     /locus_tag="ACG8N9_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4898564.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            2080..2352
                     /locus_tag="ACG8N9_003068"
     CDS             2080..2352
                     /locus_tag="ACG8N9_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4898565.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(2483..5449)
                     /locus_tag="ACG8N9_003069"
     CDS             complement(2483..5449)
                     /locus_tag="ACG8N9_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS4898566.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            5595..6170
                     /locus_tag="ACG8N9_003070"
     CDS             5595..6170
                     /locus_tag="ACG8N9_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4898567.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            6384..7079
                     /gene="vanR-A"
                     /locus_tag="ACG8N9_003071"
     CDS             6384..7079
                     /gene="vanR-A"
                     /locus_tag="ACG8N9_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4898568.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            7057..8226
                     /gene="vanS"
                     /locus_tag="ACG8N9_003072"
     CDS             7057..8226
                     /gene="vanS"
                     /locus_tag="ACG8N9_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS4898569.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRGSVAKF"
BASE COUNT         2653 a         1451 c         1598 g         2542 t
ORIGIN      
        1 ttgttttcaa ttgcttcaat tagtgctggt tatcatgtca ttattcttga aggaattgga
       61 gaaacgattg aaaactcaaa aagacaacac aaatttaccc ctaattctca tattttaatg
      121 ggaatagctg caataggtgc ttctctgata ggaaattttt gggaaggaac gttattggtt
      181 cttattttct ctggagcaca ttttcttgaa gattatgctg aaggaagaag taaaagagaa
      241 attaccaagt tattagaaat gaatccaaca acagctaggc taatcatgca agatggcaat
      301 acaacaatcg ttaatgttag tgagttgaaa gtaggagatc aacttcaagt gttaaacggt
      361 gaccaagtac cgattgacgg agtaatagta tcaggctcta cttcgattga cgaatcttct
      421 attaatggtg agagtatacc taaagagaaa tcaaagggag acggagtttt tgggagtaca
      481 attaatggaa cgggtacctt tactatggaa gtcacaaaag aaactaacga tactgtcttt
      541 tcaaagattt tacaactagt gaatcaaaat caagagaatc aaacaaaagc gtctagtatt
      601 atccaaaagt ttgagcctaa gtatgttaaa tttgttttaa ttgcaatccc actcttcatt
      661 ttacttgctc ctatgctgtt tgatttgaca tggtcacaaa gtatttatag aggattagtc
      721 cttttagtcg cagcttctcc gtgtgcttta gcagcagcta ccgtatccgt aacattgtca
      781 gccacttcta atctagcaaa aagaggcgta ctttcaaaag gaagtgccta tttgtcccaa
      841 ttagcggata ttcaagccat tgcctttgat aaaacaggaa cattaactaa agggaaaccg
      901 gaagtaacca attattactt ttctgattct gtgaacgagg aaaatatgat tgatatcgta
      961 gtggctcttg aaaaagaatc aaatcacccg ttagcaagtg ctattttaga caagtttgaa
     1021 ccaaaaaata aactagctat tgaagtggaa aatcagattg gaaaaggttt aataggcgat
     1081 tataacgaaa acacttatcg tataggtaaa ccaacttctt ttgaggctgt atcggatgac
     1141 tatacacgtt tgaataaaga atgggcttca gaaggtaata cggttgtata tgtatcagaa
     1201 aatgaaaaag taatcggcct gatagcatta atggatattc caagtgaaca tgcgaaagca
     1261 actatagatt actttaagaa gtgtggtatc cataccactt taattactgg ggattctgaa
     1321 atgacagggc aagctgttgg taaacaatta ggagtagatc aagtaattgc aaacgtcatg
     1381 ccagaagata aatctaacat tatcaatgaa caaaaagaaa cctatggtat aaccgctatg
     1441 gtgggagatg gtataaacga cgcacctgct cttgtgaagg cagatgttgg aattgctatg
     1501 ggagagggta cggatgttgc agtagatgta tctgatctag tattaatgaa aaatgatttg
     1561 tctaaattgg taaaagctca tgaaatttct ttaaaaatga atcgagttat ttggcaaaat
     1621 attattttct caatggtagt tgtcgctttt ctaattaccg taagcttagt aggattaacg
     1681 gatatagcca ttagtgtgat tgttcatgaa ggaagtacct tagtcgtgat attaaatggt
     1741 cttcgattat taaagtctaa ataaatacat gaaattggat aaaatcatgt atctaaagtt
     1801 tgcagcctct ctaattttgt attacaatgt atatacaaaa agaagggggt gattctaatg
     1861 actgtcatat cgctcagaat tgatactcag gaagataaat taattaaaga atatgctaag
     1921 gcgaagaata tttcggtttc ggctctgatt agaagttctg tcttggaaaa aatcgaggat
     1981 gaaattgatt tgggcctata taatcaagcc atgaaggatc ataaagagaa tccgaaaagt
     2041 atttcttttg aagacatgct aaaggagcta tctgattaaa tgagctcagg ttatcaagtc
     2101 gagtttgaaa aaggagctca aaaagcgtta aaaaaaatgg acaaatacca atccctttta
     2161 attatgggat ggattcaaaa gaatctagtg aattgtaccg atcctaggca gcatggaaaa
     2221 ggattaaccg tcaatcgatc aggcgaatgg cgctatagaa tcggtgatta tcgcttaata
     2281 gctgatatca atgacgagac cgtaaccatt ttaatgttag agattggtca tagaaaaaat
     2341 atttacaaat aaaccattca aattgagtgc aagcatgttt aataaaaaag gattctactg
     2401 ctttttaagg ggtagcgtca ggaaaatgcg gatttacaac gctaagccta ttttcctgac
     2461 gaatccctcg tttttaacaa cgttaagaaa gttttagtgg tcttaaagaa tttaatgaga
     2521 ctactttctc tgagttaaaa tggtattctc ctagtaaatt aatatgttcc caacctaagg
     2581 gcgacatatg gtgtaacaaa tcttcattaa agctacctgt ccgtttttta tattcaactg
     2641 ctgttgttag gtggagagta ttccaaatac ttatagcatt gataattatg tttaaagcac
     2701 tggctctttg caattgatgc tgtatggtgc gttctctaag ctcaccttgt tttccgaaga
     2761 aaatagctct tgccaatcca ttcatggctt ctcctttatt caatcctctt tgtatttttc
     2821 ttcttaatga ttcatccgat atataattca aaataaagat cgttttttct attcggccca
     2881 tctcacgtaa ggctgtagct aagctgtttt gtcttgaata ggaacctagc ttccccataa
     2941 taagggatgc tgaaactgtt ccctccctta tagaatgagc taatcgcaaa acatcctcat
     3001 aattttcttt aatgaccttt gtatttattt gtccacgtaa aatggcttct agttttggat
     3061 actcacttgc tttatctatc gtaaataatt ttgagtccga taaatccctt attcttgggg
     3121 caaatttaaa tcctaataaa tgagtcagtc cgaatatttg gtcagtgtaa ccggcagtgt
     3181 ctgtataatg ttcctctatg tttagatccg tctcatgatg taacaaacca tccaaaacat
     3241 gaatcgcatc tcttgaatta gtatgaataa tctttgtgta gtaagaagag aattgatcac
     3301 ttgtaaatcg gtagatggtg gctccttttc cagttccata atgtggattt gcatctgcat
     3361 gtagtgatga aacacctagc tgcattctca taccatctga cgaagatgtt gtaccgtcgc
     3421 cccaatagaa aggcaattgt aatttatgat gaaagtttac taatatggct tgggctttat
     3481 tcatggcatc ttcatacatg cgccattgag atacattggc tagttgctta tatgtaagtc
     3541 cgggtgtggc ttcggccatc ttgctcaagc caatattcat tcccattcct aaaagggcag
     3601 ccatgataat gattgtttct tccttatctg gttttcgatt attggaagca tgagtgaatt
     3661 gctcatgaaa tcctgttata tgggccacat ccatgagtaa atcagttaat tttattcttg
     3721 gtagcatctg ataaaggctt gcactaaatt tttttgcttc ttctggaaca tctttttcta
     3781 agcgtgcaag tgatagcttt cctttttcaa gagaaacccc atctaactta ttggaattgg
     3841 cagctaacca ctttaacctt tcattaaagc tgctggttct ctccgttata taatcttcga
     3901 atgataaact aactgataat ctcgtattcc ccttcgattg attccatgta tcttccgaaa
     3961 acaaatattc ctcaaaatcc ctatattgtc tgctgccaac aatggaaaca tctcctgccc
     4021 gaacatgctc ccgaagttct gttaaaacag ccatttcata gtaatgacga ttaattgttg
     4081 taccatcatc ctcgtataaa tgtcttttcc atcgttttga aataaaatcc acaggtgagt
     4141 catcaggcac ttttcgcttt ccagattcgt tcattcctcg gataatctca acagcttgta
     4201 aaagtggctc atttgccttt gtagaatgaa attccaatac tcttaatagc gttggcgtat
     4261 attttcttag tgaataaaac cgtttttgca gtaagtctaa ataatcatag tcggcaggac
     4321 gtgcaagttc ctgagcctct tctactgaag agacaaaggt attccattca ataaccgatt
     4381 ctaaaacctt aaaaacgtct aatttttcct ctcttgcttt aattaatgct tgtccgatgt
     4441 tcgtaaagtg tataactttc tcatttagct ttttaccgtt ttgtttctgg atttcctctt
     4501 gagccttacg accttttgat aacaaactaa gtatttgcct atcatgaatt tcaaacgctt
     4561 tatccgttag ctcctgagta agttgtaata aatagatggt taatatcgaa taacgtttat
     4621 tttcttgaaa gtcacggaat gcatacggct cgtatcttga gcctaagcga gacagctgca
     4681 acaggcggtt acggtgcaaa tgactaattt gcactgtttc taaatccatt cctcgtatgt
     4741 attcgagtcg ttctattatt tttagaaaag tttcgggtga aggatgaccc ggtggctctt
     4801 ttaaccaacc caatatcgtt ttattggatt cggatggatg ctgcgaggta ataatccctt
     4861 caagcttttc tttttgctca tttgttagag atttactaac cgtattaaat agcttctttt
     4921 cagccattgc ccttgcttcc cacaccattc tttcaagtgt agtgatagca ggcagtataa
     4981 ttttgttttt tcttagaaaa tctatgcatt catgcagtag atgaatggca tcaccatttt
     5041 ccaaagctaa ttgatgaagg tacttaaatg tcattcgata ttcactcagg gtaaaagtta
     5101 caaagtcgta ttcacttcga atttctttca aatgatccca aagtgtattt tccctttgag
     5161 gataatgatc aagcgaggat ggactaacac caatctgttt cgatatatat tgtatgaccg
     5221 aatctgggat gcttttgata tgagtgtatg gccaaccggg ataccgaaga acagctaatt
     5281 gaacagcaaa tcctaaacgg ttttcttccc tccttcgctt attaactatt tctaaatccc
     5341 gtttggaaaa agtgaagtag gtccccagta tccattcatc ttcagggatt tgcataaaag
     5401 cctgtctctg ttccggtgta agcaattctc tacctctcgc aattttcatt cagtatcatt
     5461 ccatttctgt attttcaatt tattagttca attatatatc aatagagtgt actctattga
     5521 tacaaatgta gtagactgat aaaatcatag ttaagagcgt ctcataagac ttgtctcaaa
     5581 aatgaggtga tattttgcgg aaaatcggtt atattcgtgt cagttcgact aaccagaatc
     5641 cttcaagaca atttcagcag ttgaacgaga tcggaatgga tattatatat gaagagaaag
     5701 tttcaggagc aacaaaggat cgcgagcaac ttcaaaaagt gttagacgat ttacaggaag
     5761 atgacatcat ttatgttaca gacttaactc gaatcactcg tagtacacaa gatctatttg
     5821 aattaatcga taacatacga gataaaaagg caagtttaaa atcactaaaa gatacatggc
     5881 ttgatttatc agaagataat ccatacagcc aattcttaat tactgtaatg gctggtgtta
     5941 accaattaga gcgagatctt attcggatga gacaacgtga agggattgaa ttggctaaga
     6001 aagaaggaaa gtttaaaggt cgattaaaga agtatcataa aaatcacgca ggaatgaatt
     6061 atgcggtaaa gctatataaa gaaggaaata tgactgtaaa tcaaatttgt gaaattacta
     6121 atgtatctag ggcttcatta tacaggaaat tatcagaagt gaataattag ccattctgta
     6181 ttccgctaat gggcaatatt tttaaagaag aaaaggaaac tataaaatat taacagcctc
     6241 ctagcgatgc cgaaaagccc tttgataaaa aaagaatcat catcttaaga aattcttagt
     6301 catttattat gtaaatgctt ataaattcgg ccctataatc tgataaatta ttaagggcaa
     6361 acttatgtga aagggtgata actatgagcg ataaaatact tattgtggat gatgaacatg
     6421 aaattgccga tttggttgaa ttatacttaa aaaacgagaa ttatacggtt ttcaaatact
     6481 ataccgccaa agaagcattg gaatgtatag acaagtctga gattgacctt gccatattgg
     6541 acatcatgct tcccggcaca agcggcctta ctatctgtca aaaaataagg gacaagcaca
     6601 cctatccgat tatcatgctg accgggaaag atacagaggt agataaaatt acagggttaa
     6661 caatcggcgc ggatgattat ataacgaagc cctttcgccc actggagtta attgctcggg
     6721 taaaggccca gttgcgccga tacaaaaaat tcagtggagt aaaggagcag aacgaaaatg
     6781 ttatcgtcca ctccggcctt gtcattaatg ttaacaccca tgagtgttat ctgaacgaga
     6841 agcagttatc ccttactccc accgagtttt caatactgcg aatcctctgt gaaaacaagg
     6901 ggaatgtggt tagctccgag ctgctatttc atgagatatg gggcgacgaa tatttcagca
     6961 agagcaacaa caccatcacc gtgcatatcc ggcatttgcg cgaaaaaatg aacgacacca
     7021 ttgataatcc gaaatatata aaaacggtat ggggggttgg ttataaaatt gaaaaataaa
     7081 aaaaacgact attccaaact agaacgaaaa ctttacatgt atatcgttgc aattgttgtg
     7141 gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc gagggaaact tggggattgg
     7201 atcttaagta ttttggaaaa caaatatgac ttaaatcacc tggacgcgat gaaattatat
     7261 caatattcca tacggaacaa tatagatatc tttatttatg tggcgattgt cattagtatt
     7321 cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat actttgacga gataaatacc
     7381 ggcattgatg tacttattca gaacgaagat aaacaaattg agctttctgc ggaaatggat
     7441 gttatggaac aaaagctcaa cacattaaaa cggactctgg aaaagcgaga gcaggatgca
     7501 aagctggccg aacaaagaaa aaatgacgtt gttatgtact tggcgcacga tattaaaacg
     7561 ccccttacat ccattatcgg ttatttgagc ctgcttgacg aggctccaga catgccggta
     7621 gatcaaaagg caaagtatgt gcatatcacg ttggacaaag cgtatcgact cgaacagcta
     7681 atcgacgagt tttttgagat tacacggtat aacctacaaa cgataacgct aacaaaaacg
     7741 cacatagacc tatactatat gctggtgcag atgaccgatg aattttatcc tcagctttcc
     7801 gcacatggaa aacaggcggt tattcacgcc cccgaggatc tgaccgtgtc cggcgaccct
     7861 gataaactcg cgagagtctt taacaacatt ttgaaaaacg ccgctgcata cagtgaggat
     7921 aacagcatca ttgacattac cgcgggcctc tccggggatg tggtgtcaat cgaattcaag
     7981 aacactggaa gcatcccaaa agataagcta gctgccatat ttgaaaagtt ctataggctg
     8041 gacaatgctc gttcttccga tacgggtggc gcgggacttg gattggcgat tgcaaaagaa
     8101 attattgttc agcatggagg gcagatttac gcggaaagca atgataacta tacgacgttt
     8161 agggtagagc ttccagcgat gccagacttg gttgataaaa ggaggggttc tgttgcaaag
     8221 ttttaaataa agaataaaat ccct
//
LOCUS       DAZUKD010000314         5607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.154, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000314 DAZUKD010000000
VERSION     DAZUKD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.154"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1059
                     /locus_tag="ACG8N9_003073"
                     /pseudo
     CDS             1..1059
                     /locus_tag="ACG8N9_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
     gene            1317..2819
                     /gene="eat(A)"
                     /locus_tag="ACG8N9_003074"
     CDS             1317..2819
                     /gene="eat(A)"
                     /locus_tag="ACG8N9_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4898570.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2886..3413
                     /locus_tag="ACG8N9_003075"
     CDS             2886..3413
                     /locus_tag="ACG8N9_003075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4898571.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3624..4685
                     /locus_tag="ACG8N9_003076"
     CDS             3624..4685
                     /locus_tag="ACG8N9_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4898572.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4688..5584
                     /gene="murQ"
                     /locus_tag="ACG8N9_003077"
     CDS             4688..5584
                     /gene="murQ"
                     /locus_tag="ACG8N9_003077"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4898573.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
BASE COUNT         1941 a          959 c         1255 g         1452 t
ORIGIN      
        1 ggcagttcta aatggctggg aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga
       61 tggcggagaa aatactgcag aagcaatcta ttacgcgctc aggggaacat ggcgtaccat
      121 tgatgggatg gatctcttat tccgtcagcg aaaaatcagc tatttattga caacttggca
      181 aggaaaaaac accgcagtca tcgaatctgc acaagcattg gggttagatc tacttgataa
      241 accaacggat caaactatcc gacttgcttc cagttatgcc ttaggagaac ttattaaaga
      301 tgcactgctt caagaagtag aacaaattat tgtcacttta ggaggaagcg ggtgtagcga
      361 tggtggtcta ggattactcc aatccttggg tgcttcttta gaagggtaca cagaaggaaa
      421 tcctttatta tctactaaaa aacttcattt tgatcgaaga aacgttcttg tttctaaagt
      481 aaaattgatt atcgctgcag atgttaccaa tccatataca ggtcaacaag gagctgcttg
      541 gcaatttgga agacaaaaag gaggaataga tacgacgcta gctttcttag ataaacaagc
      601 aggaaagatt gctcagcaaa taaaagattt ctgggaggtg gatttagatg atattccagg
      661 aagtggagca gcaggcggta ttggtggagc ccttttttgt tgggagcgaa gatggtttct
      721 ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc
      781 atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt
      841 gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac
      901 caagagctag aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca
      961 ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga
     1021 cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta
     1081 tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc
     1141 gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt
     1201 ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca
     1261 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt
     1321 ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg
     1381 aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
     1441 gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa
     1501 tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct
     1561 attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat
     1621 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga
     1681 agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg
     1741 aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga
     1801 aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc
     1861 atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg
     1921 aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag
     1981 aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag
     2041 gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag
     2101 gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga
     2161 tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt
     2221 tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat
     2281 tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg
     2341 tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg
     2401 cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg
     2461 caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag
     2521 tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc
     2581 ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt
     2641 tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca
     2701 atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac
     2761 atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac
     2821 ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga
     2881 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     2941 gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag
     3001 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     3061 caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac
     3121 gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc
     3181 aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt
     3241 atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt
     3301 tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg
     3361 aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt
     3421 tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta
     3481 ggttatgaca attcttttga taatataaac tcaaccttta gaaatgaaat ttaattattt
     3541 taataaaagt attgaaattt tgtttcttta gatatatact ctagtcatag aaatgaagac
     3601 ggatacagaa aaagagggat aatatgtttg gcttttcaat atttatgaac gattctttgt
     3661 cagaagagaa gaaacagtat atccgccaaa tggcatccaa tggctttgtc ggtattttca
     3721 cttccatgca tataccagaa gatgatgttt cggcatatca aaagcgattg atcgaactag
     3781 gaagcgaagc gaaaaagaat aaactagaat tgatggtcga tatttcagga gaggcattgg
     3841 aacgagcggg tttttcatta gaagatctaa cgcctttgaa agcaattggt gtaacaggtt
     3901 taagaatgga ttatcatatt tccaatcagc agattgccga ctggtcacat caattgaaaa
     3961 tcagtttgaa tgctagcacg atcacgccta aagatatcga tgaattgaaa gaagcagaag
     4021 ctgatttctc acagattgaa gcttggcata attattatcc acgtcccgaa acagggctgg
     4081 acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag
     4141 gttttgtacc aggagataca gagttgagag gcccactata tcaaggattg ccaactttgg
     4201 aagaacatcg agggatccat cctcttgcag gcgctctaga tctattagca agtaacacgg
     4261 atattgttta catcggagat gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt
     4321 tccaaaaaga acagacagtc ttgctccata cggaacctgt agatgaagaa ttttacgaat
     4381 atatattagg gaagcatacg aatcggcaag acgatgcaag agatgtcata cgaagcgtag
     4441 atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag
     4501 ggtctattac tttagataac gaaaagtatt tgcgctatat gggtgagatc caattaacca
     4561 aatatgattt gccagcggat gaaaaagtga atgtagtagc taaagttatc aaagaagagc
     4621 taccattgat caaccaaatc aaagctggta tgaactatca atttataaga aaagaaggaa
     4681 gataaaaatg agcaaaatca atttagagaa cctaacaacg gaacgcagaa accaagaaac
     4741 ttttggatta gatgagatga gtatttctca agccgttcaa ctaatgaaca aagaagatca
     4801 gaaagtggcc gctgcggttg aaaaagaact gacaagtatc gaaccagtga ttgctaacac
     4861 gattgagtcc tttaaaaaag gtggacgtct gatttatatg ggagccggaa caagtggaag
     4921 actaggtgtg ttagatgctg ctgaatgcgt accgacattc ggtgtagaac cggaaatggt
     4981 cataggtcta attgctggtg gagaagaagc aatgactgta gctgtcgaag gggccgaaga
     5041 ttcttatgag ctagggcaag aagatctaat caagctgaat ttgacaaaaa atgatatagt
     5101 tattggtatc gcagctagcg gtcgtacacc atatgtgatc ggtggattag attacgcaaa
     5161 atcgatcggt gctgtcacag gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca
     5221 cgcggatttc ccaatcgaag tcgatgcagg tccagaattt ttgacaggct ctactcgatt
     5281 gaagtcagga acagcacaaa aacttatctt gaatatgatt tctactatca gtatgattgg
     5341 tataggtaaa gtattcaata acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat
     5401 cgaacgctca aaacgaatca tcatgcaagc gacagaagct gattacgaaa cagctgaaaa
     5461 gtactttgaa gaagcagatc aaaatgttaa gctggctatc gtcatgattt tgacaaatag
     5521 tgataaagag gaagctcaag caaaattagc tgaagcaaac ggatttgtaa aagggacctt
     5581 atgaggggga aaagaccatg gcaaaaa
//
LOCUS       DAZUKD010000315         4827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000315 DAZUKD010000000
VERSION     DAZUKD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.164"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8N9_003078"
     CDS             <1..186
                     /locus_tag="ACG8N9_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4898574.1"
                     /translation="ETFEHKPEKYTEKVNHIFEVLGISLLECYDMTEKLYNEVNEIVS
                     EINNFLNEESSDERKQI"
     gene            167..901
                     /locus_tag="ACG8N9_003079"
     CDS             167..901
                     /locus_tag="ACG8N9_003079"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4898575.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            934..1842
                     /locus_tag="ACG8N9_003080"
     CDS             934..1842
                     /locus_tag="ACG8N9_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4898576.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1839..2381
                     /gene="sat4"
                     /locus_tag="ACG8N9_003081"
     CDS             1839..2381
                     /gene="sat4"
                     /locus_tag="ACG8N9_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4898577.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2474..3268
                     /locus_tag="ACG8N9_003082"
                     /note="aph(3')-IIIa"
     CDS             2474..3268
                     /locus_tag="ACG8N9_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4898578.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            3544..3675
                     /locus_tag="ACG8N9_003083"
                     /pseudo
     CDS             3544..3675
                     /locus_tag="ACG8N9_003083"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            3751..4496
                     /locus_tag="ACG8N9_003084"
                     /pseudo
     CDS             3751..4496
                     /locus_tag="ACG8N9_003084"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            4607..4786
                     /locus_tag="ACG8N9_003085"
                     /pseudo
     CDS             4607..4786
                     /locus_tag="ACG8N9_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
BASE COUNT         1653 a          740 c         1097 g         1337 t
ORIGIN      
        1 gaaacttttg aacataaacc tgaaaaatat accgagaagg taaatcatat ttttgaagta
       61 ctcggtatct cacttttgga atgctacgac atgaccgaga agctttataa tgaagtgaat
      121 gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa
      181 atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact
      241 ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg
      301 tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc
      361 ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac
      421 ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga
      481 gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt
      541 aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca
      601 tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact
      661 gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga
      721 aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag
      781 ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg
      841 gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta
      901 ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat
      961 ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca
     1021 cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta
     1081 agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata
     1141 atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat
     1201 cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta
     1261 gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt
     1321 aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag
     1381 tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc
     1441 cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg
     1501 agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac
     1561 tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac
     1621 cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg
     1681 gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat
     1741 ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat
     1801 agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag
     1861 ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     1921 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     1981 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     2041 acaatactga taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta
     2101 aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt
     2161 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     2221 aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct
     2281 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     2341 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     2401 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     2461 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     2521 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     2581 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     2641 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     2701 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     2761 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     2821 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     2881 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     2941 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     3001 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     3061 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     3121 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     3181 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     3241 ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac
     3301 tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga
     3361 tgttttatat cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt
     3421 tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac
     3481 agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa
     3541 aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata
     3601 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     3661 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     3721 cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg
     3781 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     3841 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     3901 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     3961 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     4021 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     4081 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     4141 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     4201 tggaacaaat attaaaacaa atgctttcat ataaaaaact atataatgta attcttagag
     4261 ctgaaaaagg ggagacatac aattctatca agaacaggta ttctctcggc ttccttgaag
     4321 aaaccgattt gggaagtaaa atggaaatcg aattccagac agattccttt gagatattga
     4381 gcaagcagct gattgaatat ggatcaggca ttgaaatagt ccaacctgat gaattaaaat
     4441 gcataacccg taaacacctg gcacaaatta cgaatcactg cctaaacttg atttagagaa
     4501 agcgattggt ctgttatgta ttttttgtaa ggtttcatat cccctgctga catacagctg
     4561 tcagtgtatt atgatattct actatcaaaa aggaggattg aaaaatatga aaccaaagat
     4621 aattatgcac acacaaatta gccttgatgg ccgcattaaa ggctttgata atccggaagt
     4681 ttactaccag gttgccgggg gaattcattc ggatgcggtg ctgtttgggt ccaatactgt
     4741 ttttactgca tttgaaaaat atcctgctga aacagaggct gattttggat aaagtccgta
     4801 taattgtgta aaagtaaaaa ggccata
//
LOCUS       DAZUKD010000316         4306 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.176, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000316 DAZUKD010000000
VERSION     DAZUKD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.176"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..1104
                     /locus_tag="ACG8N9_003086"
     CDS             193..1104
                     /locus_tag="ACG8N9_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS4898579.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1257..1742
                     /gene="vanZ-A"
                     /locus_tag="ACG8N9_003087"
     CDS             1257..1742
                     /gene="vanZ-A"
                     /locus_tag="ACG8N9_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS4898580.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2267..3907)
                     /gene="merA"
                     /locus_tag="ACG8N9_003088"
     CDS             complement(2267..3907)
                     /gene="merA"
                     /locus_tag="ACG8N9_003088"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFS4898581.1"
                     /translation="MNQFRMRIQGMTCTGCEKHIEKALESMGALNAKADFSQSEAVFQ
                     LPDDYAIENAIEAVKKINYQPEEVEKLPVQKRVMTNNKEDYDLLIIGSGGAAFSAAIK
                     AVEYGSKVGMIERGTVGGTCVNIGCIPSKTLLRAGEINQSAHVNIFEGLHTSTGEVEL
                     DRLVDQKNELVDDLRKQKYTNLIDDYGFDLIKGEAKFIDENTVTVSGQTYSAQRFLIA
                     TGASPLLPRITGLKEVDYLTSTTLLELRKVPKRLTIIGSGYIGRELGQLFHNLGSEVT
                     LIQRSKRLLKEYDPEISEAMEKALIEQGIKLISGATYERVEQDGNIKKVHIIVNGKNK
                     VIESDELLVGTGRKPNTEVLNLSAAGVQVGPNNEIKINAFAQTSNRKIYAAGDVTLGP
                     QFVYVAAYEGGIAAGNAIGGLNKAIDLSVVPGVSFTSPQFATVGLTQQQAKEKGYEVK
                     KAVLSLENVPRAIVNHNTTGVFKLVVDRKTQKILGVHIVSENAGEVIYGASLAVKFGL
                     TVADLKDTLAPYLTMSEGLKLAALAFDKDISKLSCCAG"
     gene            complement(3921..>4306)
                     /gene="merR"
                     /locus_tag="ACG8N9_003089"
     CDS             complement(3921..>4306)
                     /gene="merR"
                     /locus_tag="ACG8N9_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFS4898582.1"
                     /translation="SSFSEKCGVNKESIRYYERKNLLPEPDRTNSGYRVYSEEDVKRV
                     KFIKHLQEIGFSLNEIYKLLGVVDKDNIRCEGMFEFVSKKEREIQKKIENLNRIEYML
                     KDLRARCPDEKELYACPIIEVLIEN"
BASE COUNT         1337 a          762 c          744 g         1463 t
ORIGIN      
        1 acgtgagttg aaattttgac aacctaattt ttctaaggaa attggtgata atagtaaatc
       61 cagtagggcg aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga
      121 ttgtgccgtt taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg
      181 aggagactga aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta
      241 ggttatgact acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat
      301 gatcaaaatc ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa
      361 acaattacag aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct
      421 gttcgccaag aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata
      481 aatggatacg ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt
      541 tcagagatgg tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc
      601 tatcgagact ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta
      661 ccagcaggtt atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg
      721 aaaatggaac gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc
      781 attttacgtt atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat
      841 attcgctatg ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag
      901 gaatatatgg attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa
      961 tatgagatct tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt
     1021 cgttatgaga tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca
     1081 acacatacta attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag
     1141 cattattgta ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac
     1201 taggacattg ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg
     1261 gaaaaatatt atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt
     1321 tattcaaatt acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact
     1381 tgactccatt tactgctact gggaatttca gagagatgat agataatgtt ataatcttta
     1441 ttccatttgg cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg
     1501 cttttgtact ggttttaagt cttacttttg aaataattca atttatcttc gctattggag
     1561 cgacagacat aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat
     1621 atggtttaag caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag
     1681 gtatactttt gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt
     1741 aagatgtcta aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga
     1801 ttagatggaa aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg
     1861 gtgataaagt aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt
     1921 tagtgctgag gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc
     1981 tgacggtacc cctttaagcg gtagaatcct tttttgatac gatttaatta tctatattaa
     2041 gcacgtaagg caacaatcgc atcttcaata atatcttgct gcgttgccta aatgagcaat
     2101 tgcgtttcta aatagtcttt tatttcttca acctccacta gaatagagct attaattggc
     2161 acgtagacgt ttgctgatgt cataatcttc aactcctatc gttatagata ataacgaaag
     2221 ataaaccaag gtgttaccag ctactacaag aaactaaata gaaatattaa cctgcacaac
     2281 aagataattt cgaaatatct ttatcaaagg ctagagctgc taactttaag ccttctgaca
     2341 ttgttagata aggcgctaaa gtatctttta aatcagctac ggtcaatcca aatttaacgg
     2401 ctaaggatgc tccataaata acttctcctg cattttcaga aacgatatga actcccaaaa
     2461 tcttctgtgt ttttctatca accacaagtt taaacacccc tgtcgtatta tgattgacaa
     2521 tcgctctagg aacattttct aatgacaata ccgctttttt tacttcgtat cctttttctt
     2581 tcgcttgttg ttgtgttaac ccaacagttg caaattgcgg actggtaaat gatactccag
     2641 gaactaccga taaatcgatt gctttattta acccaccaat tgcatttcct gctgcaattc
     2701 ctccttcata ggctgctaca tagacaaact gaggtcctaa tgtcacgtct cctgctgcat
     2761 aaatttttct attacttgtc tgagcaaaag catttatctt gatttcattg tttggcccaa
     2821 cttgaacgcc tgctgcgctc aagttcaata cttctgtatt cggttttctt cctgttccaa
     2881 ctagtagctc gtctgactca atgaccttat ttttgccgtt tacaataata tgaacttttt
     2941 taatatttcc gtcttgctct actcgttcat aagttgctcc ggaaatgagc tttattcctt
     3001 gttcgattaa agctttctcc attgcttcag aaatctctgg atcatactct tttaaaagtc
     3061 gtttgcttct ttgaataagg gtaacctcag aacccagatt gtggaagagc tgtcccagtt
     3121 ctcttccaat ataacccgat ccaataattg ttaatcgttt tggtactttt cttagttcaa
     3181 ggagggttgt gctagttaaa taatctactt ctttaagtcc agtaatacga ggtaagagtg
     3241 gagaagcacc cgttgcgatt aaaaaccgtt gggcagaata tgtttgccca ctaactgtca
     3301 cagtgttttc gtcaatgaat tttgcttctc ctttaattaa atcaaaacca tagtcgtcaa
     3361 ttaaattagt gtatttttgt ttgcgtaaat catcaactag ttcatttttt tgatcgacta
     3421 agcggtctaa ttctacttct cccgtagaag tgtgcaatcc ttcaaaaata tttacatgtg
     3481 ctgattgatt gatttctcca gctcgaagca gcgtttttga agggatacaa ccaatgttca
     3541 cacaggttcc gcctacagtt ccgcgttcaa tcatgcccac ctttgatcca tactcgactg
     3601 cttttatagc tgctgaaaaa gctgctccac cagaaccgat aatgagcaaa tcataatctt
     3661 ccttgttatt agtcatcacg cgtttttgaa caggtaactt ttctacttct tcgggctgat
     3721 aatttatttt tttaactgct tctatcgcgt tttctattgc atagtcgtct ggtaattgaa
     3781 ataccgcttc gctttgactg aaatctgctt tagcgttgag agctcccatg ctctcaagcg
     3841 ctttttctat atgtttttca cagccagtac aggtcattcc ttgaatgcgc attctaaatt
     3901 gattcatttt cctacccccg ttaattttct attaatactt ctatgatagg acacgcgtat
     3961 agttcttttt catctggaca tcttgctcgt aaatctttta gcatatattc tattctattc
     4021 aaattctcga tttttttttg aatttctctt tctttcttag agacaaattc aaacatacct
     4081 tcacaacgaa tgttgtcttt atctacaaca ccaagtaatt tataaatttc atttaaagaa
     4141 aagccaattt cttgtaggtg tttaatgaat tttactcgtt ttacatcttc ctctgaataa
     4201 actcgataac cggaattagt ccgatcgggt tctggtagta agttttttcg ttcgtaatat
     4261 cgaatggatt ccttatttac accacatttt tcagagaagg agctaa
//
LOCUS       DAZUKD010000317         3008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.195, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000317 DAZUKD010000000
VERSION     DAZUKD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.195"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..1234
                     /locus_tag="ACG8N9_003090"
     CDS             266..1234
                     /locus_tag="ACG8N9_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4898583.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1227..2258
                     /gene="vanA"
                     /locus_tag="ACG8N9_003091"
     CDS             1227..2258
                     /gene="vanA"
                     /locus_tag="ACG8N9_003091"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4898584.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2264..2872
                     /locus_tag="ACG8N9_003092"
     CDS             2264..2872
                     /locus_tag="ACG8N9_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS4898585.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          896 a          571 c          725 g          816 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa ggaggtccta agagatgtat
       61 ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta
      121 atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt
      181 cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct
      241 ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt atggatgtga
      301 gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat
      361 aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag
      421 tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt
      481 gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag
      541 aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat
      601 gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca
      661 tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt
      721 gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt
      781 gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct
      841 gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat
      901 cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg
      961 tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc
     1021 cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc
     1081 aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca
     1141 tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg
     1201 tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg
     1261 gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta
     1321 ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt
     1381 gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg
     1441 ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg
     1501 atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt
     1561 ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg
     1621 acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg
     1681 ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc
     1741 cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact
     1801 acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg
     1861 gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg
     1921 accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag
     1981 gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga
     2041 tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg
     2101 atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt
     2161 tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac
     2221 tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac
     2281 ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa
     2341 tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt
     2401 ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct
     2461 atgggacggt taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc
     2521 ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc
     2581 aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct
     2641 ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga
     2701 acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt
     2761 gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt
     2821 attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttttaa
     2881 ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga cgtatgtaac
     2941 tggttcttag ggaatttata tatagtagat aacccgtggt gctagttaat tttaatatcc
     3001 taaagaat
//
LOCUS       DAZUKD010000318         2655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.203, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000318 DAZUKD010000000
VERSION     DAZUKD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.203"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..175
                     /locus_tag="ACG8N9_003093"
     CDS             <1..175
                     /locus_tag="ACG8N9_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4898586.1"
                     /translation="HGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYE
                     SVRLAPEKITMFE"
     gene            529..2007
                     /gene="msr(C)"
                     /locus_tag="ACG8N9_003094"
     CDS             529..2007
                     /gene="msr(C)"
                     /locus_tag="ACG8N9_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4898587.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2054..2500
                     /locus_tag="ACG8N9_003095"
     CDS             2054..2500
                     /locus_tag="ACG8N9_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4898588.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT          881 a          425 c          569 g          780 t
ORIGIN      
        1 tcatggctat gaaacaaatc caattggtgg attcgaagca gatcagttgg cagaggcatt
       61 tggtttggat aaagaccgtt atgttccagt tattatttta tcaataggaa aagcggtaga
      121 agaaggttac gagtctgttc gtttagcacc agaaaagatc actatgttcg aataaaaggt
      181 tttcagttac tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa
      241 aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac
      301 taaataaatt caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt
      361 tcgaacgaca gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt
      421 tcgttcagag taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt
      481 tttagtatcg ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta
      541 gcagtaaata taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat
      601 tctcttcgtg tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa
      661 tcgactttgc tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca
      721 gagatcacct tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat
      781 ttggaattag ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc
      841 ggaggggaag aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg
      901 cttctagacg agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag
      961 cttcgttatt attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag
     1021 ctagcatcga aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat
     1081 agtgcctatc tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg
     1141 atcatgaaag agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa
     1201 aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa tcagactgga tcggttgtct
     1261 tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag
     1321 agaagactcc aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt
     1381 ccagtaccaa aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa
     1441 ttggaaagaa gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa
     1501 aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc
     1561 aaacaaggag aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag
     1621 ggttatcaaa tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt
     1681 tcagaatcgc ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg
     1741 aaaccgttat gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt
     1801 actaagccaa gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg
     1861 atcgaagctt tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat
     1921 gattcttact ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata
     1981 aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg attttcgtga caaatttaaa
     2041 ggagtggtac agattgccta caaataaaaa agaaggaata atttttacaa caatgatgtg
     2101 ttttttgatg gtcttgggga tgagcgcgta taatttatgg ctgcatcaag atcttcattt
     2161 atcaagtttg ttgattggtc tagtgcctgg attcgtggtg gctttcattt tggatgtctt
     2221 catcatcggt gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa
     2281 ggtccaaatg atcttaacta tttcatgctt gatggtttta ggaatggtga catgtatgtc
     2341 tttattcggt gtgatcatgg aacaaggaat acctgatcaa ttattctctg cttatatgaa
     2401 tgcttggaaa atgaatgtga ttgccgcttt accgttgcag ttgatcatcg ttggcccgct
     2461 ttcaagaagt gtgctaaaag caattcaaag taatgaatga tggaacactg ttatttgaaa
     2521 aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc agttctagaa aaatagtgat
     2581 ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt cttttccaac tgtattgctc
     2641 ttttattgaa gataa
//
LOCUS       DAZUKD010000319         2193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.218, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000319 DAZUKD010000000
VERSION     DAZUKD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.218"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACG8N9_003096"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACG8N9_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACG8N9_003097"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACG8N9_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACG8N9_003098"
     CDS             complement(1055..1345)
                     /locus_tag="ACG8N9_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4898589.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2193)
                     /locus_tag="ACG8N9_003099"
                     /pseudo
     CDS             complement(1553..2193)
                     /locus_tag="ACG8N9_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          618 a          413 c          321 g          841 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgatttc tacatttgtt ttttcttgtg tcagtgttgc
     1741 gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg cttgtgcttc
     1801 ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt tttggttgat
     1861 ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat ccttgctgac
     1921 taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt tttctaattc
     1981 atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg tctgttcttt
     2041 ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca atgtatgatc
     2101 aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc acaaaataat
     2161 ttcatttgcg taccgcgtgc ctgtgtagcc aat
//
LOCUS       DAZUKD010000320         1998 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.228, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000320 DAZUKD010000000
VERSION     DAZUKD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.228"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..439
                     /locus_tag="ACG8N9_003100"
     CDS             47..439
                     /locus_tag="ACG8N9_003100"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4898590.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="ACG8N9_003101"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="ACG8N9_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4898591.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRAYKD"
BASE COUNT          830 a          153 c          344 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aagggcttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DAZUKD010000321         1531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.247, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000321 DAZUKD010000000
VERSION     DAZUKD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.247"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG8N9_003102"
     CDS             111..194
                     /locus_tag="ACG8N9_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4898592.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8N9_003103"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8N9_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4898593.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="ACG8N9_003104"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="ACG8N9_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          582 a          242 c          259 g          448 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg t
//
LOCUS       DAZUKD010000322          897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.288, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000322 DAZUKD010000000
VERSION     DAZUKD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.288"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..832
                     /gene="aac(6')"
                     /locus_tag="ACG8N9_003105"
     CDS             284..832
                     /gene="aac(6')"
                     /locus_tag="ACG8N9_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4898594.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          316 a          134 c          185 g          262 t
ORIGIN      
        1 tatggccttt ttacttttac acaattatac ggactttatc gtttttttta aagcaaaaga
       61 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
      121 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
      181 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
      241 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
      301 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
      361 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
      421 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
      481 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
      541 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
      601 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
      661 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
      721 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
      781 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
      841 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attactt
//
LOCUS       DAZUKD010000323          670 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5312
            SAMEA115707635-rid22576703.guided.300, whole genome shotgun
            sequence.
ACCESSION   DAZUKD010000323 DAZUKD010000000
VERSION     DAZUKD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707635
            Sequence Read Archive: ERR13197363
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288099.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 47 of 92
            Pseudo Genes (incomplete)         :: 43 of 92
            Pseudo Genes (internal stop)      :: 15 of 92
            Pseudo Genes (multiple problems)  :: 9 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707635-rid22576703.guided.300"
                     /isolate="VRE5312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="ACG8N9_003106"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="ACG8N9_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HFS4898595.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            206..>670
                     /locus_tag="ACG8N9_003107"
     CDS             206..>670
                     /locus_tag="ACG8N9_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N(6)-methyltransferase family
                     protein"
                     /protein_id="HFS4898596.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISIL"
BASE COUNT          275 a           76 c           95 g          224 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacattcat
      181 tataacctca taggagtggg ttataatgaa caaaaaaaat ataaaagata gtcaaaactt
      241 tattacttca aagcatcata taaatgaaat tttgagaaat gtacatttaa atacaaatga
      301 taatattatt gagattggtt cagggaaagg tcatttcacg tttgaattag ctaaaaggtg
      361 taattatgta accgccattg aaatagatcc taaattatgt aggataacta aaaacaaact
      421 tattgaatat gagaacttcc aggttatcaa taaagatatt ttacaattta agtttcctaa
      481 aaataagtca tataagatat atggaaatat accctacaat ataagtacag atataattcg
      541 aaaaattgtt tttgaaagca cagctacaga aagttattta atagtggaat atggatttgc
      601 taaaaggttg ctaaatacaa atcgttcact agcactattt ttaatgacag aagttgatat
      661 atccatatta
//
